LOCUS       DAZULG010000001        61699 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000001 DAZULG010000000
VERSION     DAZULG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.001"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG9CY_000001"
     CDS             complement(132..509)
                     /locus_tag="ACG9CY_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936553.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG9CY_000002"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG9CY_000002"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS4936554.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG9CY_000003"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG9CY_000003"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS4936555.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG9CY_000004"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG9CY_000004"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS4936556.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG9CY_000005"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG9CY_000005"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS4936557.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG9CY_000006"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG9CY_000006"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS4936558.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG9CY_000007"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG9CY_000007"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS4936559.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG9CY_000008"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG9CY_000008"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS4936560.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG9CY_000009"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG9CY_000009"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS4936561.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG9CY_000010"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG9CY_000010"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS4936562.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG9CY_000011"
     CDS             10661..11575
                     /locus_tag="ACG9CY_000011"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS4936563.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACG9CY_000012"
     CDS             complement(11708..13273)
                     /locus_tag="ACG9CY_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4936564.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACG9CY_000013"
     CDS             complement(13266..14717)
                     /locus_tag="ACG9CY_000013"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4936565.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACG9CY_000014"
     CDS             complement(14731..15747)
                     /locus_tag="ACG9CY_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4936566.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACG9CY_000015"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACG9CY_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4936567.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG9CY_000016"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG9CY_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4936568.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG9CY_000017"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG9CY_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS4936569.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACG9CY_000018"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACG9CY_000018"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS4936570.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACG9CY_000019"
     CDS             21340..22764
                     /locus_tag="ACG9CY_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4936571.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACG9CY_000020"
     CDS             complement(22806..23063)
                     /locus_tag="ACG9CY_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936572.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG9CY_000021"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG9CY_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS4936573.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACG9CY_000022"
     CDS             24337..25314
                     /locus_tag="ACG9CY_000022"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS4936574.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="ACG9CY_000023"
     CDS             25339..26982
                     /locus_tag="ACG9CY_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS4936575.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="ACG9CY_000024"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="ACG9CY_000024"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS4936576.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28558..28833
                     /locus_tag="ACG9CY_000025"
     CDS             28558..28833
                     /locus_tag="ACG9CY_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS4936577.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28839..29606
                     /locus_tag="ACG9CY_000026"
     CDS             28839..29606
                     /locus_tag="ACG9CY_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS4936578.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29774..31603
                     /gene="typA"
                     /locus_tag="ACG9CY_000027"
     CDS             29774..31603
                     /gene="typA"
                     /locus_tag="ACG9CY_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS4936579.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31864..33623
                     /gene="recQ"
                     /locus_tag="ACG9CY_000028"
                     /pseudo
     CDS             31864..33623
                     /gene="recQ"
                     /locus_tag="ACG9CY_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
     gene            33737..34039
                     /locus_tag="ACG9CY_000029"
     CDS             33737..34039
                     /locus_tag="ACG9CY_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS4936580.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34042..35205
                     /locus_tag="ACG9CY_000030"
     CDS             34042..35205
                     /locus_tag="ACG9CY_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4936581.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35231..38659
                     /locus_tag="ACG9CY_000031"
     CDS             35231..38659
                     /locus_tag="ACG9CY_000031"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS4936582.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38680..39804
                     /locus_tag="ACG9CY_000032"
     CDS             38680..39804
                     /locus_tag="ACG9CY_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS4936583.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39785..40231
                     /locus_tag="ACG9CY_000033"
     CDS             39785..40231
                     /locus_tag="ACG9CY_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4936584.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40215..40553
                     /locus_tag="ACG9CY_000034"
     CDS             40215..40553
                     /locus_tag="ACG9CY_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS4936585.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40672..41232
                     /gene="rsmD"
                     /locus_tag="ACG9CY_000035"
     CDS             40672..41232
                     /gene="rsmD"
                     /locus_tag="ACG9CY_000035"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS4936586.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41229..41720
                     /gene="coaD"
                     /locus_tag="ACG9CY_000036"
     CDS             41229..41720
                     /gene="coaD"
                     /locus_tag="ACG9CY_000036"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS4936587.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41710..42753
                     /locus_tag="ACG9CY_000037"
     CDS             41710..42753
                     /locus_tag="ACG9CY_000037"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS4936588.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42912..43640
                     /locus_tag="ACG9CY_000038"
     CDS             42912..43640
                     /locus_tag="ACG9CY_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS4936589.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43756..44001
                     /locus_tag="ACG9CY_000039"
     CDS             43756..44001
                     /locus_tag="ACG9CY_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4936590.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            43998..44216
                     /locus_tag="ACG9CY_000040"
     CDS             43998..44216
                     /locus_tag="ACG9CY_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4936591.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44352..44891
                     /locus_tag="ACG9CY_000041"
     CDS             44352..44891
                     /locus_tag="ACG9CY_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS4936592.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44884..47157
                     /locus_tag="ACG9CY_000042"
     CDS             44884..47157
                     /locus_tag="ACG9CY_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS4936593.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47195..48241
                     /gene="holA"
                     /locus_tag="ACG9CY_000043"
     CDS             47195..48241
                     /gene="holA"
                     /locus_tag="ACG9CY_000043"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS4936594.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48330..48584)
                     /gene="rpsT"
                     /locus_tag="ACG9CY_000044"
     CDS             complement(48330..48584)
                     /gene="rpsT"
                     /locus_tag="ACG9CY_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS4936595.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48984..49253)
                     /locus_tag="ACG9CY_000045"
     CDS             complement(48984..49253)
                     /locus_tag="ACG9CY_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936596.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49515..49643)
                     /locus_tag="ACG9CY_000046"
     CDS             complement(49515..49643)
                     /locus_tag="ACG9CY_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936597.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49723..50178)
                     /locus_tag="ACG9CY_000047"
     CDS             complement(49723..50178)
                     /locus_tag="ACG9CY_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS4936598.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50316..52244)
                     /locus_tag="ACG9CY_000048"
     CDS             complement(50316..52244)
                     /locus_tag="ACG9CY_000048"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4936599.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52441..53274)
                     /locus_tag="ACG9CY_000049"
     CDS             complement(52441..53274)
                     /locus_tag="ACG9CY_000049"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS4936600.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53466..54170)
                     /locus_tag="ACG9CY_000050"
     CDS             complement(53466..54170)
                     /locus_tag="ACG9CY_000050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4936601.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54193..54819)
                     /locus_tag="ACG9CY_000051"
     CDS             complement(54193..54819)
                     /locus_tag="ACG9CY_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4936602.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55145..57520
                     /locus_tag="ACG9CY_000052"
     CDS             55145..57520
                     /locus_tag="ACG9CY_000052"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS4936603.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57544..58035)
                     /locus_tag="ACG9CY_000053"
     CDS             complement(57544..58035)
                     /locus_tag="ACG9CY_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS4936604.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58222..59142
                     /locus_tag="ACG9CY_000054"
     CDS             58222..59142
                     /locus_tag="ACG9CY_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4936605.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59296..59640
                     /locus_tag="ACG9CY_000055"
     CDS             59296..59640
                     /locus_tag="ACG9CY_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS4936606.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59830..60330)
                     /locus_tag="ACG9CY_000056"
     CDS             complement(59830..60330)
                     /locus_tag="ACG9CY_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4936607.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60858..60941
                     /locus_tag="ACG9CY_000057"
     tRNA            60858..60941
                     /locus_tag="ACG9CY_000057"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60892..60894,aa:Leu,seq:cag)
     gene            complement(61265..>61699)
                     /locus_tag="ACG9CY_000058"
     CDS             complement(61265..>61699)
                     /locus_tag="ACG9CY_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4936608.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20237 a        10661 c        13216 g        17585 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28441 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28501 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28561 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28621 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28681 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28741 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28801 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28861 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28921 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28981 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    29041 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29101 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29161 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29221 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29281 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29341 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29401 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29461 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29521 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29581 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29641 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29701 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29761 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29821 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29881 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29941 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    30001 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30061 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30121 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30181 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30241 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30301 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30361 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30421 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30481 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30541 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30601 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30661 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30721 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30781 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30841 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30901 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30961 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    31021 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31081 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31141 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31201 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31261 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31321 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31381 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31441 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31501 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31561 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31621 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
    31681 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa
    31741 gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga
    31801 aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg
    31861 aagatgaacg acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa
    31921 gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt
    31981 gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg
    32041 tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc
    32101 gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc
    32161 gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt
    32221 atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc
    32281 tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa
    32341 ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct
    32401 gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga
    32461 gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat
    32521 ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac
    32581 cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta
    32641 aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt
    32701 gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat
    32761 tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat
    32821 ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt atccagcagt
    32881 cccaaagaga agaagggcaa aagcaaaaag aatatgaaaa attaaaagcg atgacagaat
    32941 acgtgtatat tgagtcctgt cttcagcaat acatcctaaa ctattttggc gagacaagca
    33001 gtccgtgcaa ccgttgtggt aactgtttag atgacagaga aatagtagaa gtaacgacag
    33061 aagcacagat ggttctttct tgcttaaaac gtatgggtga gaactatggt aaacaaatgc
    33121 tgatgaaagt cttagcagga tcgaaagaac aaaagctgca agctttaggt tttggacatt
    33181 tatctaccta tggactgatg aaaaaccaat cgcaaaaaga aacgatgcag ttaattgaat
    33241 atttgatatc gaatggctat cttctgacca tcaatggtga gtatcctgtt ttgaaagtaa
    33301 cagaaagagg gatacaagta ttgaaaggac aagaatctgt ttatcgaaag gaacccaaga
    33361 aagtccagca gctatcggat gaagaaaccg atacactatt tgaagtgctg cgggaactgc
    33421 gaacggatct agcatcagaa gcaggcgttc ccccgtatgt cgtattttca gattccacat
    33481 tgaaagaaat gagccgaata cgtccatcaa gcagattgga gatgcttcag ataaaaggag
    33541 ttggccaaag caagcttgat aaatatggcg aagccttttt atctcggata aaaaatgcgg
    33601 actctaacgt tctggataag tagaaagaac gaatacgtaa aaatgaataa aaacaactgg
    33661 ttaaatctgt tttttttgct ccttatgaaa atcaaatatg ttataattaa ggaattagaa
    33721 tgggaggtaa gcacagatgg attctttgtc gaaaaaactt gcgatccaag tattaaatga
    33781 tgaagcagaa cggatcaaac gattaatccg aaatcaaaac aacagtctct gtatctcaca
    33841 atgcaaagcc ttcgaagaag tggtagatac acagatgtac ggcttttccc ggcaagtcgg
    33901 ttttgctgtc cgtataggag taatcggaaa gaacgagggc cagctgatgt taagtgagct
    33961 agaacgagaa ttgaatcagt tatatagtga ggtttaccaa gaaatttatg ataaaaaaga
    34021 agttggcaag gaggaataga cttgcctaag aaagtgaaaa aaaggcatct attagactat
    34081 agcatcttga ttccctattt aattttatgt gtattgggat tgatcatggt atatagttct
    34141 acgtcgtatc tattattaga aaacggacaa aatccgtccg catcagtcat taaccagagt
    34201 attttttggg tgcttagctt gattgctatc gcactacttt ataaaatgaa aacagatgtt
    34261 ttgaaaaacc agcgtttgat catggcagcc atcgcagttt tgacgatttt gctgctgatt
    34321 gttgtgtttt ttggtaagga gatcaatggg gcgaaaggtt ggctgcaaat tgcaggattc
    34381 tcgatccaac ctgccgaata cctaaaaatc atttctatct ggtacctgtc tctgacttta
    34441 tctaaaagac aaaacagtgt acaaaaagat tttcttggga ctgtgaaacg tccacttgca
    34501 atggttatcg gacttactgc attagtcgct attcttcccg attttggtaa tgcggcagtt
    34561 atctttttga ttattttggt tctgctttta gccagcggag tcaattatgt gtatactttg
    34621 attgtaggag taggaggctt ttgtctaagt acttttacga tctggctgat caatatcaca
    34681 aacggaaaga tcttacccgg acgtttacag tatatctata atcgatttgc tatttaccaa
    34741 aatcctttct cagacgaatt aaataaaggg catcaactag tcaacggcta ctatgccatg
    34801 ttcaacggag gactatttgg caggggatta ggtaacagta tccagaaaaa aggattttta
    34861 caagaagcac aaaccgattt tatttatgca atcgtagtgg aagaactggg agtgattatg
    34921 gggattttga tattagcgtt gctattcttt atgattgtgc gcattgtcct tgtaggtatc
    34981 cgttcaaaag atccgttcaa ttcgcttttg tgcattggga tcggcgcaat gtttttgatc
    35041 caagtatttg tcaatcttgg aggaattact ggcgttattc ctttgacagg gatcacgttc
    35101 ccatttttaa gtcaaggagg ctccagtttg ctgatgcttt cgatttgtgt cggctttgtc
    35161 ctcaacatca gtgcggatga aaaacgcaag agcttaggtt tataataaaa aaagatgtag
    35221 gagtggttcg atgaaaaaag ttttagttgc taacaggggt gaaatcgctg tacgtgtatt
    35281 tcgtgcttgt acagaattag gaattaaaac tgttggaatc tatgcagaag aagatgaata
    35341 ttccgtacac cgtttcaaag cggatgaagc atatttagta ggacaaggaa aaaaaccgat
    35401 tgatgcgtat ctagatattg aagggatcat ttctatcgcg aaagaatgcg gagctgacgc
    35461 tattcatccg ggttacggtc ttttgtcgga aaacttgaat tttgcgcaac ggtgcgaaga
    35521 agaaggcatc atttttgttg gacccaaact gcatcattta gatatttttg gagataaaat
    35581 caaagcaaaa gctgcagcta tcgaagcggg gattgcttcg attccaggca cagatggacc
    35641 aatcgcttct atagacgatg ccttggagtt tgcgaaacaa tacggttatc cgattatgat
    35701 caaggcagct ctaggcggcg gcggacgcgg catgcgtgta gctcacgatg aaaagagtgc
    35761 aagagagggc tacgaacgtg cgaaaagcga agcaaaagca gcctttggta gtgatgaagt
    35821 gtatgttgaa aaatacatcg ctaatcctaa gcatatcgaa gtacagattc tcggggatac
    35881 gcatggaaac gtcatccatc tttttgaaag agattgttct gttcagcgcc gtcaccaaaa
    35941 agtagtagaa gtagcgcctt gtgtctctat gaatgaacag caacgtcaaa aaatctgcca
    36001 ggcagctgtc caattgatga aatatgtcgg gtatgtcaac gcaggtacag tagaatttct
    36061 tgttgaagga gatgattttt actttatcga agtcaaccct cgcgtccaag tggaacatac
    36121 gatcacagaa atgattacag atattgatat cgtaacaacg caactgttga ttgctcaagg
    36181 tttggatctg cataaagaaa tcggattgcc tcagcaagaa ggaattaaat taaatggttc
    36241 agccattcaa tgccgaatca ctacagaaga cccgttgaat aactttttac cagacacagg
    36301 gaaaatcgat acgtatcgtt cacctggtgg ttttggcgta cgtctggatg tcggaaatgc
    36361 ttacgcgggc tatgtagtga caccgtattt tgattcattg ctagtaaaag tatgtacgca
    36421 tggtgctacc tttgaaacag ccattcagaa aatggaacgt tgcttgaaag aatttagaat
    36481 ccgtggagtg aaaacaaaca ttccatttat gctcaatgtc atcacacatc ctgaattcca
    36541 atcaggaaat gcaaaaacaa ccttcattga tagtacagca acattatttg aatttcctcg
    36601 actacgagac cgcggaaaca aaacgatgaa atatatcggt gaaattactg tgaatggttt
    36661 tccaggtatc gaaagtggcg aaaaaccatt ttatgaagaa ccacgtatgc ctaaagatct
    36721 aatcacaaga actgattacg taacggctaa gaatgtatta gatgcgaagg gtgccgatgc
    36781 tttggtcgaa tggatcaaag gacaggaaaa tttgttgttg acggatacga catttagaga
    36841 cgcccatcaa agtctgctag caactcgtgt tcggacaaag gatttcaagc agattgcacg
    36901 tttgactgga gaaggtcttc ctgaactctt ttcaagtgag atgtgggggg gagcaacatt
    36961 tgatgtggct taccgctttt tgaatgaaga tccttggcaa cggttgagaa aaattcgttc
    37021 tttaatgcct aatacgttgc tgcaaatgct tttcagaggt tcaaatgccg ttgggtattc
    37081 taattatcct gacaatgtat tggtggaatt tgtcaaagaa gcagcagcgc aaggaatcga
    37141 tgtatttcga atctttgata gtttgaactg gactccacaa atggaaaaaa gtatccaagc
    37201 tgtacgggat actggtaaaa tcgcagaggc agctatttgc tatactggcg atatcaacga
    37261 tccaagtcgg gcaaaataca atgttcagta ttataaagac atggcaaagg aactagaaca
    37321 actgggtgcg catatcattg caatcaaaga tatggctggg ctgcttaaac cgcaggcagc
    37381 ttatcgtttg attagtgaat tgaaagaaac gacagatctg ccaatccatc tgcatactca
    37441 tgataccagc gggaatggaa tcatcactta ttctgctgct tcaaaagcag gagtagacat
    37501 cgttgacgtg gcaatgagtg caatgagtgg gaatactagt cagcctagca tgagcagctt
    37561 gtattatgca ctggtcaatg gtccacggct gccagaaatc acgattgaaa atgcacagaa
    37621 attaaaccat tattgggaag atgtgcgcat gtattacaaa ccgtttgaaa acggcttgaa
    37681 tgctcctgaa acagaagtat atatgcatga aatgccagga ggacagtatt cgaatctaca
    37741 gcaacaggca aaagctgtcg gattaggtca tcgctgggat gaaatcaaac aaatgtatca
    37801 tactgtcaac ttgatgtttg gcgatatcgt taaagtaact ccatcatcta aagtagttgg
    37861 agatatggca ctatttatgg tccaaaatga tcttacagaa gaagatattt atgaaaaagg
    37921 cgaaacacta agtttccctg aatctgttgt gactttcttc caaggagaac tagggcaacc
    37981 cgttggcgga tttcctaaaa aattgcaaaa gattattctt aaaggcagac ctgcattgaa
    38041 tgaacgtcca ggtcttttgg ctgaatcagt cgacttcaac gaagtaaaaa aagaattggc
    38101 agaaaaaatt ggctatgaac caaaacaaga cgaagtatta agctatttga tgtatccgca
    38161 agtctttttg gactatcaaa aagcatataa tcaatttggc gatgttacat tactggacac
    38221 accaacattt ttccaaggta tacgtttggg tgaaacaatc aatgtacaaa tcgaacgcgg
    38281 aaaaacgctt atcattcgtt tagatgaaat cggagaaccc gatatcgaag ggaatcgcgt
    38341 actgttcttc aatttgaatg gacaaagacg tgaaatcact atcaaagata attcgatcat
    38401 cagtacggtc caaacccgat tgaaagctga accgacaaac cgagaacaaa ttggagcaac
    38461 gatgtctggt tctgttttgg atgtattagt gaaaaaaggc gataacgtta aaaaaggtga
    38521 tacgttgatg attactgaag cgatgaaaat ggaaacggcc atcgaagcac gttttgacgg
    38581 agaagtcgca catgtatatg tgtcttcagg agacactatt tcgtctggtg accttttgat
    38641 tgaagtgact gagaaataat aagcgaaagg tggggaaaca tgaaaagact gggtttgttt
    38701 tggctgattc tgctattagt tattgtggca ttctacatgg aaccagtctt gtttccgcca
    38761 aaacttgaag tgaaggaaaa ctctgatcaa caaatatcaa accaaatgac aacgtggaaa
    38821 taccaggagc ttaaagcaac tggtttttct acttatatcg gaaaaccgat cgatcagctg
    38881 gaaaaggatt ttggccaagc ctatgatcga ttaactagcg gatttggttt tgaaacaaga
    38941 tattatgaaa ataaagctag tcatctcaca tttgaagcga acatccaaga cagcagagtc
    39001 acaactgtta aagtactgca aacagggcct gctgatatcg cgccgtttta tttagggatg
    39061 acgatgcaag atcttacgaa aatcactact atctatacta atttcacttt tgagtaccat
    39121 gatacagaag ttggaatcga actaatggaa gaggatatga attatcgtcc gctaattgct
    39181 tttgataacg acacttttgc tattttattt tttgatcaaa caacaaacgg cctattttca
    39241 gttgtgtatt tagataaaga tagtcttttg aaactattgc catatcaagt gtttggtgaa
    39301 ggccttccac attatcaagc agataaatcg gctgattggg aatcaatcaa tcgcgttaaa
    39361 gaacgaaaaa gtacgacttt attaaatatg cttagaagag aagacgaact tcccatatac
    39421 aatcaaacac ttgcctttca aaaccacagt cagctgctac tacatgattt tttgacaagg
    39481 cctgaggaaa tattgtcaga ggaacgtatg gctgattggc aaaaatcgct aggcagtgct
    39541 caaacgacag tgaaattctc actgacgaat gatgaattgg agaagttaat cagtaaacaa
    39601 aaattagaaa atactagtgg gctatttatt catccggcga ttgacgctac tttttcgttt
    39661 ttatattggt acagcgatcc ttacaaccat gaccgtttca tggacccggg aacggattct
    39721 gtaggcattg ctttttctaa agaaaatatg ttagtcttat tgcaagaaga aaacggagaa
    39781 agtagtgata gcagtgatcg ttaacgaaga gctgtttcaa ctggaggatc aatgcctcca
    39841 agtcgctgaa atgataaaaa agagtaaaac gatggcttct tatcttgaac ataaacagtc
    39901 aatggaagaa tctgcaaaag tttgccagtt gaaagaagcg ttcttgtcta agaaagaagc
    39961 atttgaaaat gtagcggcat atggtgagta tgcacctgat tatcgtgaaa aaaaacgagc
    40021 tgttcgaaaa gcaaaaagac agcttgatat ggatgagcat gttgcagctt tccgatatag
    40081 tgaaacacag ctgcaaagtc tgttggatca aattgtccaa gtacttgccg aagaagtttc
    40141 taatgaaatc aaagtagatg caggtagccc gttttttgaa acgaaaaaac actttggatg
    40201 cggaggtaat tgtcatgcaa ttagacaata gtgaatttga aataacaaaa cgacgcgggc
    40261 tgatcgtatg ggtctacagc ttaaaacaat taaagaatct gaaaaagttc ggcttgttgc
    40321 attatgtctc aagaagaatg aaatatgtcg tgctttactt aaatgaagag acattggaag
    40381 aaacagaaga acgaatccgt aaattacatt ttgttcgaaa cgtagaacgt tcttaccgac
    40441 cggatatcga aatgaatttt gccgaaaaaa taggaaacaa aacagtatta aaagacgaag
    40501 gtttcgaaat tgaagagcta aatacagaaa tccgattagc tgaacatctt taaaaatctc
    40561 ttatgagcag tttttaaata aaaaataact tggataacga tcgatttttg atcattgtcc
    40621 aagtattttt ttggtatact agtaccagta ccatttctaa ggagtgagta gatgcggata
    40681 atatcaggaa attatggcgg gcgccgttta aaaagtttag caggggcgaa caccagacca
    40741 actacagata aagtaaaaga atccatattc aatatgattg gaccatattt tgatggagag
    40801 acagtattgg atctgtttgc aggaagcggt ggtttagcta tcgaagcagt atctagaggc
    40861 tgcagtcatg cggtatgcgt agacaaaaat taccaagcgc taaaaatcat caaagaaaac
    40921 atcgaaatta cgaaagaacc agagaaattt accacaataa aacttgatgc ggacaaagca
    40981 atcgaacggc tagcagcgga gaaacaacaa ttcgactact tgtttcttga tccgccatat
    41041 gcaaaacaaa aaataatcga tcaaatccat aaaatggaag aactgggact tttttctaaa
    41101 accgcagtta tcgtatgcga aacggataaa acagtcgaat tgccagaaac tgtggctgga
    41161 ttccatcaaa taagaaaaca gatttatggc ataacggccg taactatcta tagaaaagag
    41221 ggaaatcaat gaaacgtgca ttattcccag gaagttttga tccttttaca aaaggtcact
    41281 tagatacggt ggaacgtgcg gctaaattat tcgatgaggt agtaattggt gtttttatca
    41341 atacttcaaa gaaaagtttg tttcccccag aagaaagaat gacattgatc acaaaagctg
    41401 tttctcatct tcctaatgta aaagtcatgc atcaagaaaa tcaattgacg gtagaaacag
    41461 caaaagagat tggtgcagat gcgttgatca gaggcatacg aagtatcaaa gactttgaat
    41521 atgaacgaga gattgctcag atgaatcatc atctatatga tgaattggaa actgtcttct
    41581 tattagcaaa accagaatat agtcacgtga gttccagtat tttaaaagaa gtactgcact
    41641 ttggcggaga tgtgagcagc tacttaccgc cagtgatcaa cgaagcttta gctggaaagc
    41701 gtgaatcaaa tgaaagctaa acaatggttg aaaacagtac tgattatcat atttggaatg
    41761 ttgatcattg gatttgtagt agttcccttg ccgtattatg tagaatcacc gggagccacg
    41821 atcgatctga aagaactaat tacggtcaat gataaggaag ataaaggttc tgggtctttc
    41881 tctcttacct ctgttggtat caggcaagct acaggtttct tagctttaaa agctaaaatg
    41941 accccttttg aagaattgat cagtgaagaa gaattgactg gtggggcgac aaatgaagaa
    42001 tataatcaaa tgcaacagta ttatatggat tcttcccaaa atgcagcaat cgaacaagcg
    42061 ttaaaacttg cagacaagcc ttataaaatg gaattcaaag gcgtatatgt catgagtgta
    42121 gagaaaacat cgaatttctt tggcaagctt tcagttggag atacagtaac aaaagtagac
    42181 gggaaaagct ttcaaagtac agaagcattt atggattatg taaaaagcca aaaagttgga
    42241 caaacaatcg aaatcagtta tttgcataat ggtgaagaaa aaacagcttc tggtgatctg
    42301 atcgagttac caacagataa aaaggcagga ataggtatca cgcttactga ccataccgag
    42361 atcgaatcag atacagatgt tcgtattaat tctggctcta tcggtgggcc atcagcggga
    42421 ttgatgttca ctttagaaat ctatgaacaa gtcactggta aaaacttgcg acatggtaag
    42481 caaattgcag gaactggaac gatcaacagt gaaggagaag ttggccgaat cggaggaatc
    42541 gataaaaaag tggcaagtgc ggataaagct ggcgtagaga ttttctttgc accagatgat
    42601 acgatttcag acgatgttaa gaaagaatat cctgaaataa aaaccaacta cgaagaagca
    42661 aaagctgccg ctaaaaaatt gaattcctca atgaaaatcg taccagtaaa aacagtacaa
    42721 gaagcgcttg actatctaga gaaaatgaaa tgaaactaaa taattaaaga ggggttgtga
    42781 cacgagttct gtcacaaccc ctctttttcg tcaaaaatct tcacgataaa aataaaaaca
    42841 aaagaaaaaa ttgacgtaat tagatgttga agggacgatt ttgccagagc tcactgtttc
    42901 ttcaatttta aatgaaagga gtaatgaatg atttttactg gtcattcttt gaaaaaagaa
    42961 caatgataga aaaaatcagc aagaaaatac tagcttcacc caaattgatc atttggatgt
    43021 ctatgatagt tcttatcttt tgtatcgcaa gtggtttttt attatcaaat ttgctatttt
    43081 atagagaaac agaagagact gctgcaaaag aaatactttc ttcgtttagt tccagtggag
    43141 aagatccatc agctgaagga aacgatgtag aagaggatgc tcgtccaaag gtcatgtaca
    43201 ccgatatcaa aggaagtgtg aaggagcctg gtatctattc cttttcttca gaagaacggg
    43261 tctatgatgt actaaagcgt gcaggtggat tattagaaga agcggatagc gatcgaatca
    43321 acttttctgc gaagatagaa gatcagcaag tcctatatat ccctgctgta ggtgaagagc
    43381 tgccagaaca tcttgatcag ttggcatcct cggaaggaaa acagcctaca gcagattccg
    43441 agcctcccaa aataaatatc aatacagcca ctccatcaga acttcagcag attcctggga
    43501 tcggatcagt caaagcgcaa gaaatcattc gatttagaga agaaaacgga tccttccaga
    43561 aagtggaaga cctgcaggag atttctggta tcggtgaaaa gacagttgaa aaactaaaaa
    43621 acttcgttac aattaaatag aatgattcaa aatcgactgg cacgagcaat aaatggacgc
    43681 taatggatac taagaataat ccaattctaa gagcttggca ggacgaggag gtagtatttt
    43741 tggataggaa atagcttgat tgttcgcaaa gaaacattaa aaaaaccaat gctgaatgtc
    43801 tatctccaaa ataaaatatc tgggattcat attatgaata cggctgtcag cggcaataac
    43861 agtcaagctc tacgagaacg ctttgccaaa gatgtactta gctatacagc agataaagta
    43921 tttattctca ttgggacgaa tgatctagca gagcataaac aactttctaa agaaacgtac
    43981 caaaaaatct gcagtggatg atagttcagc tgaaaaaaga ctatagttct gatcatgctt
    44041 tctttatcac gccaccagca gtcgatgaga caaaacaaaa atatcggaat aacaagttga
    44101 tcaaagaata tatctctgtt ctcacagaag tagcaaaacg ccatgattgt gaagtatcga
    44161 tttttatacg gaatttcttg aacaaatgga ttcgaagaat ttttataatg ggctaatgga
    44221 tgacggactt catttcggca gtgaagggta ggatcttttg tcagacagga tgattcgtta
    44281 tatttaaaga atattttgtt taagcaatga gtacttttta atcaacttga aaaaacggag
    44341 gaatataaaa catgttagaa aaatcttcaa aagcatgcga tacgaataaa gaaaaagtta
    44401 ctgataagtc acataatgaa cgcattcctt gggaccagta ctttatggct caggcagtac
    44461 tgttatcttt acgtagtacg tgtacacgtt tagaagtagg agcgacactc gttcgagaaa
    44521 aaaggatcat tgctggcggc tataatggag cagtcagtgg agatgtccac tgcatagatg
    44581 aagggtgtta cgtcgtggat ggacactgct tgcgaacgat ccatgcagag atgaatgcac
    44641 tcctacagtg tgcaaaactt ggtatcccga cggaaggttc agaaatctat gtcacacatt
    44701 ttccatgttt ggcctgtaca aaggctttgt tgcaagctgg tgtgaaaggg atcaattatc
    44761 tccatgacta tcgaaatgat ccttatgccc aagcgttgat cgaacaatta ggtgtatctg
    44821 tacaccaagt tgctttagac tcacagcatt tttcaaaact tcaaaatgaa ttaacaggaa
    44881 aacatgtcta gcgttttaga aaggcatgtt cgaaatcggt tgatttttcc ggcaattacg
    44941 tcgatgatta tctgtttgtt gtattacact gacaattggg gttatcgttt gcttcttgcc
    45001 ggaatttttt tatggttttc gctaaaagaa ggacgtttta tcgcgtactt gtgtattttg
    45061 tgtagcataa cggtatccat ttcttatagg ctacaagagt attcattttt agaggaaaaa
    45121 acaacatctg cgcaaattac tgtatttaca gatacgattc gtgcaaatgg taattttttg
    45181 acaatggaag gaaggttatc agagaataaa caaaagctcc agctttctta tcaagtaaac
    45241 acagaagcag aattgaaaac atggcttaac ttccgaggaa agggaataga attgactgct
    45301 gaaggcgagt tcatctctcc agcaaaaaag cggaatcttt ataattttga ccaagaagct
    45361 tacttcagga accatagaat ttctggacgg ttccaaattc aaaagattga acagatcacc
    45421 atgcaaaatc actggcgaaa ctggcttcag agaaaaagag gatcttttat ctcctttgtc
    45481 caatctgaat ttccgcaaaa aacaagtgta tatattaatt ctttattatg gggctataaa
    45541 gatgcaggat tcagagaacc agaagaaatt tttagggaga gtggtttgct gcatctgttc
    45601 agtctatcgg gtctacacat ccaattttat ttagggtggc tgtattattt gtttcgacgt
    45661 atcggatggc cactacacgt cagtattatt ccgttgagtc tactaactat ctgttatgtt
    45721 tctattgcag gaagttctat ttctgttttg cgtgctagtt tgctgttttt attgcgattg
    45781 ctagtcaaat tgctgaataa gcattattcc accatggact tatttgcagt tgtcttttgg
    45841 cttcttttac tatttgatcc tcgatttctt tttcatgcgg gtggacagct tagttttttc
    45901 atgagttttt tgtttatcct gatttctttt gatggagatt ggttgaaaaa aacggcctct
    45961 cacgtcctgt ttactatgat cacgatgccg ttgattgtat ggcattttta tgagtggcct
    46021 attttaggta gtctttttac attactgatt gcaccggtat tcaagtttct ttttcttcct
    46081 ggtgtgttat ttctcacact cttcaatcgc tggctgccgg aaactttact tttgctgata
    46141 gaagagggat taatcttatt tgagcgaata atcgattttt cttcagggtc gtctctcatt
    46201 ataggacgtt tgccccttgt gctatctggg atgtatattt tcggattact gatttgtatg
    46261 gataggttga aagaacaacc tgtgaaacac ttgttttatg ttggtctttt ttcttttatt
    46321 tttttgactt tgccattttt acgcttttct tcaacgatcg cttttatcga tgtcggtcaa
    46381 ggagacagta tctttttgca aagtcctttt cgaaaagaaa cgattttgat cgatactgga
    46441 ggtcagttac actttgaaag agaaaagtgg gcgcaaggta tgatacgtcc tcctgctgaa
    46501 agaaatatcg ttccatatct aaaaggtcag gggataagtg taatcgataa actcatcttg
    46561 acacacgacg atacggatca tgtgggagaa ttacccacgt tatctcagca ttttactatc
    46621 aaaaaaattt atatcggttg gggcgctgga cgaaatgagc cattacaaag gcatttatct
    46681 gaagctcaaa aaaatggtac tgagatcatt gaaatcaaac atggggacag gatcagcggt
    46741 tattatgatc tttatgtatt gactcctttt gaaaagggag agggaagaaa tgaagattcg
    46801 attggtctat ggatggaata taacaatagg cgctttttat ttttaggaga tttgaatcaa
    46861 gcaatggaaa aacaattgct tttgatttat cctaatctga aagccgatgt agtcaaatta
    46921 ggtcatcacg gtagccgtac gtcaagtaac acagattttc tttcccatat tgaagcaaaa
    46981 catgggatta tatcctgcgg cgtaaataat cgttatggtc acccgcacag cgaagtattg
    47041 gagacactag aagaaaatca gataaaaaca tggcgaacgg atcaacaagg aatgatcacc
    47101 tatcgctggc atccagtttt tcatcctcat gggctagtgg aaacaatgat agactagtaa
    47161 ttctcatctg ctcatggtag gataattttt gaaagtggtg agtaatttga gtatacaagc
    47221 agagttacaa aaaatacgta aagaaaaact tgcatcttgt tacttgattt taggaacaga
    47281 aaagtttctc caagatcaag ttcggacaga aattgtcaaa aaaatccaat tagagggcga
    47341 agatgactta aattttttaa gttttgacat ggaaaatgca agtttagatg aggtagtagc
    47401 tgaagcagag acgttgccgt ttttcggaga tcaacggtta gtattcgtag agaatcctta
    47461 ttttttaaca ggtgaaaaag taaacaatgg aatcgaacaa aatactgatt tattgacggc
    47521 ttatttgaaa gaaccattag aatcgactgt attagtattc tttgcgccat atgaaaagtt
    47581 ggatgaacgg aaaaaagtaa cgaaacaatt gaaaaaaaca gcaattatca tcaatgtcca
    47641 acagttgaat gaaaaagaag tacgtcaata tctgatgaat accttagaga attcctcaat
    47701 caaaatggaa cgaagtgcaa ttgatttatt cttgaggttg accgacttag atttatccaa
    47761 aatgatgaga gagttacaga aattggtcct ttatggacaa aatcaacagc aaatcacggt
    47821 aagagaagta gaagaattag ttcctaaaac gttagaacat aatatttttg acatgaccca
    47881 atatatttta aaagggaaga cggaacaagc tttgcgttta tacgaagatt tagttatgca
    47941 aggggaagaa acaatcaaaa ttaatgcgat tttgctttct caattacgtt tatttttaca
    48001 gacaaaattt cttgtaaaga ttggttatca gcaggcaaat attgctgaaa cattgaaaat
    48061 ccatccatat cgtgtaaaac ttgccatgca ggaagtaaaa aaattcgatg aacatttgct
    48121 ggtacagttg tttgatcaat tagttgagat ggattatcaa atcaaaacgg gacaaatcga
    48181 aaaagaatta agttttcaac tattcgtttt gcgcacaggg cagttgttaa gaaatcgata
    48241 agaagaaaga cgagggcaaa gcatgcggga aggtcgtttg tttttgtcct ttttttgaca
    48301 aataaaaaaa tcggccgaag ccgattcttt tatttagcga tttttttgct taaacgagat
    48361 ttgtcgcggt tagctttgtt tttgtggatt agaccttttg attcagccat atcaatagct
    48421 ttaacagctt ctttatataa cgcatctacg ttgtcagcac ctgaagctac agcatcttca
    48481 aattttttga ttgcagtacg catagcgctt gtttgagatg aatttttcac attagcgttt
    48541 tcgcttgtac gaacacgttt gattgcagat tcaatgtttg gcattagttt cacctccgat
    48601 aacttaagtt cttacgcgtg ttaccgcata attcaacatt cattattata cctaaaggca
    48661 gcgtgctttg caatatgaac atgacaagtt tttttcctta acagtctttt attaggattt
    48721 tttttcagta aaaacggata gtacaagctg aaaaaaatca aaaagacgct attgtgggag
    48781 gcaatagcgt caaaccaaac tagattttta tttatctatt taaacaaagt ttgaatggga
    48841 atgtttggta ctaagttatt atataacaaa gagaaccgtt tgcaaagttt tttttgaaaa
    48901 gggatacgat tccctgttgt tctttgtttt tctattaaga aactgcagtt attttataac
    48961 cagtatcctt tgatcagtaa aggctattct ggtgtaccga ttattttaat gtattcatcg
    49021 atatttttta tcaacatttt ttttatgacc aactggttcc gacggttcca tttgccactt
    49081 acttccagca catatttatt taggggcaaa cgtaagatat attttgccaa atagcgatct
    49141 ttaagcaggc cattgatcac acggtcctca agctgcaagt tgatcccagt cagcatcatt
    49201 ggctgttctt gtaaaacgta cgtatcaatt aaaagacctt caaaagtttc cacaagcgtc
    49261 ttcctttctt ttcaaagtaa cctgaaaaca ggcactaatt cttccatttt gatattcgtt
    49321 gaagtggatg cgatcattct ttcttttgtc cactgtaatc tagtataaca aaaaagagca
    49381 gaagagccaa aaagaaaaaa gagcatcgaa ttctatagga agtctcattg gcacggccta
    49441 aattattaca aaacattgat taaaaaaagc ccctgcatcg caggagcatc ccctatatcg
    49501 ttttaatagg tcaatcaaaa agcagtatca aacacatttt gtatcaaatc aggaaataat
    49561 agataagcta agcccagaat caaaagtacc gctagaagaa accagccgat tgttcctatg
    49621 aaatacttta atttttcgaa cattgtatca cctcgttgtc agtatatcac ggatcagtaa
    49681 attgggggtg ttaagaatct cctaaaatag acaaaatttg ttctattttc ctaaagcaaa
    49741 tacttgtaag atacagtaag tgataccaga agcaatgacc ggtccggcgg cgattcctct
    49801 taagaagaca acacctaaaa tcgtgccgaa aacaagtgca acagtgattt gtggatcaga
    49861 agcaatcaat cctacaccct tggaagataa gacagccact agcccaccgc aaaaaacagc
    49921 tacccagcca ataggggatt taaaggcttc taatagatca cgtagaccga tttctcctgt
    49981 tgcgataggg attagaatag ccgctgtgat gatcgtcacc ccaatattga ttcctttgcc
    50041 ttgaagccaa gggaaaaagg aacgggaggc tggtatgagc ttcataatca aaacaaaagc
    50101 tgccgctatc atcagacttt gatttttagc taaaattgct acaagtaaaa taattcctaa
    50161 aaaaagccaa ctttccatga aatatattcc tcactttctt cgaaacaatc ataacatgtt
    50221 tttagaaagt tacaaaagta ggaattcatc cttcaagacc tattcataag ctgttttttt
    50281 cataaaataa acaaaatcat aaaaaaacac tttgcttatt tgacagaaga cagccgcgct
    50341 ttgtatagtg gacaagactt acattttttt gaagaatcag aaagatcagg taaaggtctt
    50401 agtaaacgca agccattcag aataaccaat attgtgcttc cttcgtgtcc tacaactcca
    50461 agaggtagat ttaaaaattg caggaagtta gaagcaatca acaagagaat aacaagaatc
    50521 gaaaaaataa tattttgttt gatgatccgc cgcattttta aagatagccg atgactgtag
    50581 acaaatttat ttaagtcgct tttcattaag acgatgtctg cgatatccat agcggcatct
    50641 gtcccttgcc ccatagcgac acctaaagaa gcggctgcta aagcaggcgc atcattgact
    50701 ccatcaccaa ccatcgcatt tacttggtaa agttcattct cttttttgac tagttgtgtt
    50761 ttttcttccg gagtacagct tgcatagtaa tgttcaatcc ctacttgttc agcaatggcc
    50821 tttgctgtgc cagaatggtc gccggttatc atacttgtat ggatgttttg ggatttaaag
    50881 taggaaatta cttgttgggt atttgctttt ggaatatcta gcagacctaa aacagcaatt
    50941 aactgctgat ctttagaaag ataaatcacg gttttgcctt gattttccaa acgttcgatc
    51001 atttccgcaa tctctttgct gattatcatc gtctcaggat catatgcgtg cttgccaact
    51061 ttccattgcg cacctaaata ggtagtttgt aatccaaaac cagtaatttc ttctattggt
    51121 aagtattgga tttcttcagg tattgtgcaa tcgaaatatt gcaaaatcgc atgagcaagt
    51181 ggatgcgtcg ttttttgttc catagcaaca agaagttgtt gtgccaaatc cttttcatca
    51241 aagaaaaagc tgtcagttac aactggtttt cctttagtga gcgttcctgt tttatcaaag
    51301 gcaatcgctt ttaattcaga aagcttttct aaatggatac cgcctttgat taaaatccca
    51361 ttttttgcag aatttgaaag agcagctaga gaagctggtg tagcagatgc gacaagcgca
    51421 caaggagaag ccacaactaa taataccatt cctcgataaa agctttcatc ccaactccaa
    51481 cctaggaaaa agtgggggag aaggatcatt agaggaacag caagcaaaat caattttaca
    51541 taattatttt cgattcgttc gataaatgaa gctgtttgag ttggcgtatt ctgcgcttct
    51601 tctactaatt ggataatctt agcaaagaca gtttcggaac tcgtttttgt tactcgaatc
    51661 gtgatgggat ttccttgatt cagcgttcct gcatacatct catcgcctaa atatttttct
    51721 acaggcacag attcaccgct taatgcagac tcatcgatcg ttgcctgtgt gctttctaaa
    51781 aagccatcta tcggaacagc tgctccttta gcgacaaata ccaagttatc cacctttagt
    51841 tgagaaacat ctacttcttg catttgtcca ttttctttta ataattgggc tttttgtggt
    51901 tgaagattca tcaaagcagt aatttctttt tgacttttat tagttgtgta ttcctctaat
    51961 gcaccgctta aacaaaagat gaatgtcaac attgctcctt caaagtaatt accgatcaaa
    52021 caggcaccaa tagcagctaa agccatcaat aaatccacat tcagtgtccg atcattccac
    52081 aattctttca agccttccga tgtttgttta tatccaccga tgataatggc actgatataa
    52141 aaaagtgggg tgatggtaag agagaatcgg tcgaagataa aaccgaagat cataaataaa
    52201 aggcagccga ttgttgctgc aaaagctttt ttctgattag tcatcgttat tagacctcct
    52261 tgtcaaaaca aggatagcat aataaaaatg taattgataa taataacagc taaatgagaa
    52321 cgataatggt tatcatttgg tgattaagct tgatgttgtt tttattattt ataatgatta
    52381 ttattaaaga aaataaaaaa ccagaacatt tctcaaataa gagaagtgtt ctggtttttt
    52441 ctacattgca ataaaccaat aaccaatcaa aatgatacca aggataatac gataccagcc
    52501 aaaagcggta aagtcgtttt tcttcaaata gcttagtaag aatttgatag ctaagacgga
    52561 aaccacaaaa gatacgaatg ttcctacaag aagaacaaca gtttcttcca ttttgaatga
    52621 attcccttca gctaaatatt tgacgatctt caagccgctt gcgccaaaca tgacaggaat
    52681 ccctaagaaa aatgaaaact ctgtagcgac aaaacgcgaa gcaccaatca agatagatcc
    52741 taggatcgtt gcgccagaac gggatgttcc gggaacaagt gccaagactt ggaataaccc
    52801 aacaagaaga gctgcttggt acgtaaagtc attcagagaa gtccaacgag gtgttttgtt
    52861 tttgttgcgt ttttcgacaa taataaaggc aataccgtaa acgatcaaaa caatggcaac
    52921 tggtaagaac ttgtggaaat ttgcttctaa ccaatcatcc aaaggcaaac caatgatgat
    52981 ggctggtatg caagcaacga tcactttaaa ccaaagtgtc cacgtatctt ttttttctac
    53041 cgttgattta cttggagaaa atgggtttaa cttgtgaaaa taaagaacga cgacagccaa
    53101 gatcgcccct aattggataa cgacattgaa catcgacata aattgtgaac tggcatccag
    53161 tttgatgaat tcatctgcta ggatcaaatg accagtacta ctgatgggca gccattccgt
    53221 tattccttca ataataccga gaatcactgc tttgataata tttgcaaaaa acataaattc
    53281 cttccttccc aattaagata catcttatag tataccgtta ttttttgaga aacccaatct
    53341 gttttttaat attaagaaaa acttgagaaa taaagcagat aagtacacga agagaaaaaa
    53401 gaagacaaag ccctcgttca gactttgcct tttatgattc tctggtaaaa attaaaatat
    53461 ccccattata gaaacgagtc gcgactatgt gttttcccgt tatcgatata agacgtatca
    53521 ttgatgccgt ggatagaaaa tcgaccgttt gcataagtga cctttgttac gctgccattt
    53581 aaaagcccag cacgaacagg ctgtttagga tcgatcagat taagcaaaaa ggcgatcgtc
    53641 aaaccatgag atacgactaa aacatttcca ccaccatttt tttcgcagtg atgggcgata
    53701 tcttcaaatc ctgtccatac acggtctcgc aatgtttcat aagtttccgc ccacccagcg
    53761 gtatctaagt tgtatacagc attagcaatt tgttcaaaag aaacttctgt ggtaaacatt
    53821 tcatcatatg ttttaaaatt caatacacga gggatcacac cccacatttc catgtcatat
    53881 ccaccttcaa gagacccgaa acaccattct ctaatccttt tttcaataaa ataaggaatt
    53941 tcttttccct tagaatgttc tcctagaata atacgggcag tttcgattgc acgaccactg
    54001 tcactagaat aagcaaatga aaagtcgaca ttttttaacc cgcgacctaa ataatgaatg
    54061 ccttcttgtc cttgctttgt gaggggtgta tcacaccagc cttgtactcg ttcaattgta
    54121 ttgaacatcg tttttccgtg tcgtacgata taaagtgtag tttctgtcat tttattctcc
    54181 tcctaagtcg ttttaacgat tgaaaaaagg atttgtcctt ttttcctgtt caatcgtcgt
    54241 tgcatcgcca tgtcctggat agactgcaaa ttcatcaggt aatacaaaca gctgggtccg
    54301 aatactatga agtaattgtt ctaaattccc tgtatacaga tcggttctac cgatacttcc
    54361 tctaaacagg gcgtcgccag aaacgacaaa atcatcaaaa ataaagctga cgcttcctat
    54421 agaatgccct ggggtaggga cgacgcgaaa agacatgttt cctaaagtat aagttttcat
    54481 ttcaaattca tattctgcag gcgagacaat aacatcagca atatcatcat gtctaccgag
    54541 tccagaaaga ttcaaaatcg gatcacctag ccatttttgc tctagtgggc tgacataaac
    54601 tgggatttga taatacgcgc gtaaaggttc cactgcaccg atatgatcgt agtgagtatg
    54661 ggtaagcaaa atcgctaccg gctgttggtt tgtttttttg atttcttgtt tgattcgttc
    54721 cgcatcttct ccagggtcaa tgatcaaaag tgcttcttcg ttgtagacca aataacagtt
    54781 ttcttcaatc attcctgttt ttaatctttc gatatgcatg tgcattcctc cttgatagcc
    54841 aaaacttctt tcatccatta gtataacgca ttcattgcta ggaaacaaaa acaggtactt
    54901 atttctcttt acttatctga attatcagac tatatgaaaa gtatttggat attctaaaga
    54961 gaaggaaagt aagttttcat agcattttaa tatgtccttg ttacattaga ttcatccaaa
    55021 aatccaaatt ctatatccta tatccctgcc taatcagcag ggtgtttttt tatgtttgga
    55081 aagagagctc tttttgctaa actaggtttt ttcaggtaat cttagagaga aagaggtggt
    55141 ggaaatgatc aaacagtcaa aaatatctgt gcggcactta gtagaattta ttttgcgacg
    55201 tggaagtatc gacaatcgta aaaaaagtaa ccatactgca ttagaaggag cgagaatcca
    55261 ccgcaagctc caaaaagatg cgggagaaaa ctatgaaaaa gaagtctttt taaaaacaac
    55321 agttcaatta gatgaatatt acctgatcgt tgaaggccgt gcagacggta ttttcaaaag
    55381 agacggtatt tattatatag atgaaatcaa aacctccgaa ccacgatttg aagatttaga
    55441 accagaacaa gtggaacttt tctttcatca agcaagggtg tatgcctata tctatagtca
    55501 agaacatgat ttacaagaaa taaatctgca attgacttac ttccagacgg cagaagaagt
    55561 gatcacgaga aaggttgaac atcagacaag agagcagtta gaaacttttt ttaaaaagct
    55621 gactgaagat tataaagaat ggcttgtttt tcaagaaaac tggcgcactg tacgcaatgc
    55681 ctctttgatg gctttgaaat tcccccatga tgagtatagg aaaggtcagc gtgaactagc
    55741 ggtagctgct tataaaacga tccgtacgaa acagaaattg tttgtagaag cacctaccgg
    55801 aacaggaaaa acaatctcta cgttattccc cgctttgaaa gctgtagggg aagaagaggg
    55861 agaaaagatc ttttatctaa cggctaaaac aataactaga caagtagctg aagatgctat
    55921 gacagcatta aaagatacag gagctgaagt aaaaagtgtc actttgacgg cgaaagacaa
    55981 gatctgcttt ttagatgaga cgacctgtaa tccagatcag tgcccatatg ccaatggtta
    56041 ttacaatcgg attaatgaag gtttatggga tcttttgaat catgagaatc agatcactcg
    56101 tgaagtcatc gaaacttatg caagaaagca tacactttgt cctttcgaac tatcattaga
    56161 tgtcagtatc tggtgtgacg taatcattgg ggattacaat tatttatttg atccaactgt
    56221 ttatttaaga cgtttttttg aagatgaaaa aaacgaggac tatctgtttt tgattgacga
    56281 agcacataat ttggtgaatc gttcccgaga gatgtattcg gcagaactat cttatgaaaa
    56341 aacgaaacga agtaaggaag ctataccaaa ggaattcaaa aaacttcatc gccgtttcaa
    56401 caaactatta aaagaatttg attcgatccg agaaatagca aaagaagatc attgggatta
    56461 tcatcatcaa aaagcaccgg ctgaatcttt agtaaaggca ggttatcaat taagcgaaaa
    56521 aattaaagaa tggctggcag aatttcctga gcacccatca caagaacaat tgcttcctta
    56581 ttattttgat ttgcttcatt ttttgaaggt cagtgaattt tatgatgatc actacgaaac
    56641 aacagtcgaa aaaacgtatc gcgatctgat agtaaaagag ttttgtattg atccaagctt
    56701 gtttcttgag cagtcgttgg ataaaggaaa aagcagtatt cttttttctg ctagtttttc
    56761 accactctct tattatcagg aaacactggg gggacaaaaa agtctggctt acaaactgcc
    56821 gagtcctttc ccagaaggaa accagcaagt tttcatcgca aattacctag agacgactta
    56881 tcgaaaaagg gaacacagtc tggcaaaatt agtagagacg attcatgctt ttgctgatgg
    56941 aaaggtcggg aattatttcg ttttctttcc ttcttatcaa tatttagatc ttgcggtgga
    57001 agcattcaga cagcaatatc ccgacagcaa tgtgctaatc caagaaacgg acatgaatga
    57061 ggaagaacgc gaacgatttt taacaaaatt tcaaacaaat cccgctgaaa cattgattgg
    57121 attttgcgta cttggcggga ttttttcaga aggaatcgat ttgagagggg accgattgat
    57181 cggcagtatg atcgtaggtg taggtttgcc gcagatgaat catgagcagg aactgatcaa
    57241 atcatattat gatgagaagg aacaccttgg atttgcttac gcttatcagc ttcctggaat
    57301 gaacaaagtc ttgcaagctg gtggtcgagt gattcgtggc atggcggatc aaggaatcgt
    57361 tgtattggca gatcaacgat tttctagttt ttcttatcga cgtttattcc cacaacattg
    57421 gcaaacagca agagaagtac gaagtgttgg ggaattggaa gaaggaatac agcgtttttg
    57481 gttatcaaaa gaagcagtaa atcaaaaaaa cgaggaatga cagacgtcag cctcgttttt
    57541 tgattaacga tagttagtga attgcaactc gattggtaag tcaagtttac gtaataaagc
    57601 aatcacagct tgtagatcat cacggttctt acctgtcact ctaatctgat cttcttggat
    57661 ttgtgctttt actttcagtt tttcattttt gatagctgta gtgattttct tcgcgttttc
    57721 acgatcgatt ccgttgacca aatcggcagt ttgtcgagct ttgccaccta gagcgtgttc
    57781 tgattcggaa aaatggatat ttttgattgg cacatttctt ttgatcagct taccaaataa
    57841 tacatctttg atctgttcga ttttgaaatc atcatcgctc aatacaacta gattgctgtt
    57901 ctctaatttg atttctactg tagaatcctt gaagtcaaag cgattggaca attcttttgt
    57961 tgtttgctga atcgcatttt ttacttcttc tgtattgact tctgatacaa tgtcgaagct
    58021 agcatctttt gccataggaa ccctcctgat atacatataa atatttacca tacatctatc
    58081 ttatcatagg attagggcag attcttacct gtttgctaaa attcttctca tttgagttcg
    58141 aagtatttta ttttaataga taaactgtta ttcttacatt tggaatacca aagtagggga
    58201 gaggtgcttg tgagtaaaat tatgactgcg tatcataaaa atgaaagatt aaagaaaata
    58261 gtggttattt tgataaccgg attgaccgct gcggtcgctt taaattattt tttgattcca
    58321 gccaatgtct tttctgcagg gatgaatggg atcgctcaaa tcatttcctc gcttttatat
    58381 gaatggtttc atatcaagat agacacagga acatttattt ttcttttgaa tatccctgtg
    58441 tttgtattag ggtttctcaa agtaggaaaa cgagcaacga tccttagttt tgtgaatgtt
    58501 gtatgtgtat cggtgatgac gatgctgctt ccagtcggac agatcactga taatattttg
    58561 atgaatgccc tagtaggcgg tgttttatta ggtcttggcg taggattatc tttaaaaatg
    58621 ggctttacaa ctggtgggat ggatatcatc tctcttgtac tttcacaaac aactggaaaa
    58681 acagtcggaa actacatgct gattctaaat ggtatcattg tagtcatcgc aggcttcttc
    58741 tttaactggg aaagtgcctt gtatacgatt atttccattt atgcaatgac tcaagtaatt
    58801 gatgccatcc acacgagtca tcaaaaagta acagcaatga tcgttaccgt caagccagaa
    58861 gaggtaacag aagaactagc aaaacggatg gtacgtggaa tgacattact gccgtctgtc
    58921 ggtggctatt caaaaaaaca agggaacatg atcatgatgg tcatcacccg ctacgaaatg
    58981 tatgatcttg atcagatcgt tcatgaaatc gacgacgatg catttatcaa tattttacca
    59041 actcattcag tatttggccg gtttgcaaat gaaaacgaac aaaaaatgta tcgaacaact
    59101 ggcgtattgc cagaaataaa aaatcgttca caaaaaaaat gaggaatcag gtttatttta
    59161 gaataaattt aatctattac atctaataag aacattttta taaatttaaa cgagaatctt
    59221 acttctaaat tctcatttac aagcagctaa aaaaagagta tgataaataa aaatcgtgtt
    59281 aaggagtgtc gtacgatgag cgaagcaaac caagaacggt ctggacaaga aatagaacta
    59341 atcaaagaac gtattcttgc tgccttggaa atggtgattg atcctgaatt aggaatcgac
    59401 atcgtcaatt taggcttgat ttatgatata gaattcaatc ctgaaaatgg agaaacagtc
    59461 attaagatga ctctaacaac tatgggctgt ccattagcag atattttaac agaatctatc
    59521 catgacgcat taaaagaagt gccagaagta acgaaagcag aagtcaaact agtctggtat
    59581 ccagcatgga cgacagataa gatgagccgt tatgcacgga ttgcgttggg gatacgatag
    59641 aaatcttggc tgactagcta tgagaagcaa ataggaaaaa caaaaaagcc ttttatcctt
    59701 tctagaagaa aagagataaa aggtttcttt gtttataaaa ttttgtctgc tatattataa
    59761 gttaattaaa taaatgtgtt ctattgataa aggacattca aatccctttc cttttggaat
    59821 ccttcttttt tacatcagtt tgattttcgt atagtattct ttctcatctt cacgataaat
    59881 cgcagcccca tttttttgtt gtgctttaag actgccaaga ttttttttat ttacagacaa
    59941 aagtgcctca tcaatgccga ttttttttgc ctcttgtagt actaatgcta atccttgtgt
    60001 ggcatatcct tttttacgat aattagggga gatgccgtaa ccaatatggc cagcgccgtt
    60061 tcttaatgtt tcgttcagtt tatggcgcaa attaaaaatt cctacatact catgttcatc
    60121 actttctaaa ataaagacgg ttgcgggaac gtaactctca ggaagatcga ttcctaaaga
    60181 cgttttttcg cattttttta cataatctaa aaattcagca aatgtatagc cataagcatt
    60241 gttgataaat ccactctcat ttggtccaaa gctagtttgt agctgataag ctttctttaa
    60301 atctgcttcc catagtttga tcagtttcat cgtattcacc tctttaagta accttattca
    60361 tttgttttct actatagcac tattgttgta aatagccatg cctattttta tttattgtgt
    60421 catatcgtat taagaatcat cgaacaatac aaatatgaat tacaataaaa aaattatttg
    60481 aagttcgttc attcgttact tatgatctct agaagaaaga aaacgtgaat tacttcagcg
    60541 tctagaattc cagaaggata gtaatcaaca gaagaaataa tcaatatcca aatgaaagaa
    60601 gatagctgaa tcgagaaaaa gaggaaaaaa gaaatgaaga aaaaaataaa atagttacta
    60661 atcccagagt cgttcgttta acattgaaat cgatcatatg gataaaacag attttttcac
    60721 caaccctcaa tagatgatgg agttgctcgg atgaattatt tgtgtagatg gcataaggga
    60781 gggcgtacag aaatgaaaaa gaacaaaaat atgttcttga caaaaaacac atgtaatcct
    60841 aaactaaaag atgtgatgcg gctatggcgg aattggcaga cgcgctagct tcaggcgcta
    60901 gtgagggtga cctcgtggaa gttcgagtct tcttagccgc ataatttgtt tttttataaa
    60961 gagaaggtac acctggagcg atgagaaaga aaataacata ttatctaaaa gaatatagga
    61021 aaaattttaa acaacggaac cagattcaaa taaatcaaaa aaaatttgtg tgaaggtggg
    61081 acagtttaaa acctaataga ttgattcaaa tagtagattt atagattctg atacaagaaa
    61141 tactggcaaa cgatagttaa ggaataaaga ctaaacttaa actcaaaaag aggtcatgcg
    61201 aaagaataaa tcgcatgacc tcttactata ttttgaatgt gtttaaacgt tcaaataatg
    61261 aatcttagta ccaaccgttt gcttcccaga atgctttcgc cgcttcccaa gaaccataac
    61321 gtgaagtaac atattgttct gctactcttt cttggtttgc agcagagtag tcaccattta
    61381 agtaagaaga atctaattgg taacggccga tgtaacgtcc gttcgttgct gtgtaagaac
    61441 cgcttgattc tttttgtgcg atccattctt ttgcagagct tgtacttgct ggagtagcag
    61501 cagtttctgt tacagcagga gcaggttgtg tttcagcaac aggcgctgct tgtgtttccg
    61561 tttgaaccgt ttcttgtaca acaggttgtt cagcttgaac aggttcagct tggactggtg
    61621 cttcttgaac ggctgcttga ggttcagcag ctgcttgtgc agagccttct gttgggattg
    61681 ttaattgttg tcctgagta
//
LOCUS       DAZULG010000002        59346 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000002 DAZULG010000000
VERSION     DAZULG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.002"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..590
                     /locus_tag="ACG9CY_000059"
     CDS             72..590
                     /locus_tag="ACG9CY_000059"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4936609.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            718..1401
                     /locus_tag="ACG9CY_000060"
     CDS             718..1401
                     /locus_tag="ACG9CY_000060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS4936610.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1710..1783
                     /locus_tag="ACG9CY_000061"
     tRNA            1710..1783
                     /locus_tag="ACG9CY_000061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1744..1746,aa:Arg,seq:tct)
     gene            1942..2301
                     /locus_tag="ACG9CY_000062"
     CDS             1942..2301
                     /locus_tag="ACG9CY_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS4936611.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2424..4055
                     /locus_tag="ACG9CY_000063"
     CDS             2424..4055
                     /locus_tag="ACG9CY_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS4936612.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4117..4758)
                     /locus_tag="ACG9CY_000064"
     CDS             complement(4117..4758)
                     /locus_tag="ACG9CY_000064"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4936613.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4944..5020
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5040..5348
                     /gene="rplU"
                     /locus_tag="ACG9CY_000065"
     CDS             5040..5348
                     /gene="rplU"
                     /locus_tag="ACG9CY_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS4936614.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5375..5719
                     /locus_tag="ACG9CY_000066"
     CDS             5375..5719
                     /locus_tag="ACG9CY_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS4936615.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5732..6025
                     /gene="rpmA"
                     /locus_tag="ACG9CY_000067"
     CDS             5732..6025
                     /gene="rpmA"
                     /locus_tag="ACG9CY_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS4936616.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6459..9266
                     /locus_tag="ACG9CY_000068"
     CDS             6459..9266
                     /locus_tag="ACG9CY_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4936617.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9457..9948
                     /locus_tag="ACG9CY_000069"
     CDS             9457..9948
                     /locus_tag="ACG9CY_000069"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4936618.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10051..11040
                     /locus_tag="ACG9CY_000070"
     CDS             10051..11040
                     /locus_tag="ACG9CY_000070"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4936619.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11073..11876
                     /locus_tag="ACG9CY_000071"
     CDS             11073..11876
                     /locus_tag="ACG9CY_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS4936620.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11897..12811
                     /locus_tag="ACG9CY_000072"
     CDS             11897..12811
                     /locus_tag="ACG9CY_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4936621.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12894..13268
                     /locus_tag="ACG9CY_000073"
     CDS             12894..13268
                     /locus_tag="ACG9CY_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS4936622.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13407..13652
                     /locus_tag="ACG9CY_000074"
     CDS             13407..13652
                     /locus_tag="ACG9CY_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4936623.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13824..14888
                     /locus_tag="ACG9CY_000075"
     CDS             13824..14888
                     /locus_tag="ACG9CY_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS4936624.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15134..15559
                     /locus_tag="ACG9CY_000076"
     CDS             15134..15559
                     /locus_tag="ACG9CY_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4936625.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15552..16001
                     /gene="nusB"
                     /locus_tag="ACG9CY_000077"
     CDS             15552..16001
                     /gene="nusB"
                     /locus_tag="ACG9CY_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS4936626.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16098..16952
                     /locus_tag="ACG9CY_000078"
     CDS             16098..16952
                     /locus_tag="ACG9CY_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS4936627.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16953..18290
                     /gene="xseA"
                     /locus_tag="ACG9CY_000079"
     CDS             16953..18290
                     /gene="xseA"
                     /locus_tag="ACG9CY_000079"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS4936628.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18295..18519
                     /locus_tag="ACG9CY_000080"
     CDS             18295..18519
                     /locus_tag="ACG9CY_000080"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS4936629.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18521..19411
                     /locus_tag="ACG9CY_000081"
     CDS             18521..19411
                     /locus_tag="ACG9CY_000081"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4936630.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19420..20238
                     /locus_tag="ACG9CY_000082"
     CDS             19420..20238
                     /locus_tag="ACG9CY_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS4936631.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20345..20794
                     /locus_tag="ACG9CY_000083"
     CDS             20345..20794
                     /locus_tag="ACG9CY_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4936632.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20812..22494
                     /gene="recN"
                     /locus_tag="ACG9CY_000084"
     CDS             20812..22494
                     /gene="recN"
                     /locus_tag="ACG9CY_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS4936633.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22549..22665)
                     /locus_tag="ACG9CY_000085"
     CDS             complement(22549..22665)
                     /locus_tag="ACG9CY_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS4936634.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22738..23694)
                     /locus_tag="ACG9CY_000086"
     CDS             complement(22738..23694)
                     /locus_tag="ACG9CY_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4936635.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23865..24233
                     /locus_tag="ACG9CY_000087"
     CDS             23865..24233
                     /locus_tag="ACG9CY_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS4936636.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24403..24834
                     /gene="mraZ"
                     /locus_tag="ACG9CY_000088"
     CDS             24403..24834
                     /gene="mraZ"
                     /locus_tag="ACG9CY_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS4936637.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24855..25814
                     /gene="rsmH"
                     /locus_tag="ACG9CY_000089"
     CDS             24855..25814
                     /gene="rsmH"
                     /locus_tag="ACG9CY_000089"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS4936638.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25819..26220
                     /gene="ftsL"
                     /locus_tag="ACG9CY_000090"
     CDS             25819..26220
                     /gene="ftsL"
                     /locus_tag="ACG9CY_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS4936639.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26221..28413
                     /locus_tag="ACG9CY_000091"
     CDS             26221..28413
                     /locus_tag="ACG9CY_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4936640.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28439..29401
                     /gene="mraY"
                     /locus_tag="ACG9CY_000092"
     CDS             28439..29401
                     /gene="mraY"
                     /locus_tag="ACG9CY_000092"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS4936641.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29415..30776
                     /gene="murD"
                     /locus_tag="ACG9CY_000093"
     CDS             29415..30776
                     /gene="murD"
                     /locus_tag="ACG9CY_000093"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS4936642.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30802..31890
                     /gene="murG"
                     /locus_tag="ACG9CY_000094"
     CDS             30802..31890
                     /gene="murG"
                     /locus_tag="ACG9CY_000094"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4936643.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31905..33125
                     /locus_tag="ACG9CY_000095"
     CDS             31905..33125
                     /locus_tag="ACG9CY_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS4936644.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33281..34606
                     /gene="ftsA"
                     /locus_tag="ACG9CY_000096"
     CDS             33281..34606
                     /gene="ftsA"
                     /locus_tag="ACG9CY_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS4936645.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34629..35870
                     /gene="ftsZ"
                     /locus_tag="ACG9CY_000097"
     CDS             34629..35870
                     /gene="ftsZ"
                     /locus_tag="ACG9CY_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS4936646.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35887..36564
                     /locus_tag="ACG9CY_000098"
     CDS             35887..36564
                     /locus_tag="ACG9CY_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS4936647.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36578..37177
                     /locus_tag="ACG9CY_000099"
     CDS             36578..37177
                     /locus_tag="ACG9CY_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS4936648.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37258..37479
                     /locus_tag="ACG9CY_000100"
     CDS             37258..37479
                     /locus_tag="ACG9CY_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS4936649.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37528..38310
                     /locus_tag="ACG9CY_000101"
     CDS             37528..38310
                     /locus_tag="ACG9CY_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS4936650.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38331..39038
                     /locus_tag="ACG9CY_000102"
     CDS             38331..39038
                     /locus_tag="ACG9CY_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS4936651.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39300..42098
                     /gene="ileS"
                     /locus_tag="ACG9CY_000103"
     CDS             39300..42098
                     /gene="ileS"
                     /locus_tag="ACG9CY_000103"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS4936652.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42147..43673)
                     /gene="zwf"
                     /locus_tag="ACG9CY_000104"
     CDS             complement(42147..43673)
                     /gene="zwf"
                     /locus_tag="ACG9CY_000104"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS4936653.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43688..44335)
                     /locus_tag="ACG9CY_000105"
     CDS             complement(43688..44335)
                     /locus_tag="ACG9CY_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4936654.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44519..44848)
                     /locus_tag="ACG9CY_000106"
     CDS             complement(44519..44848)
                     /locus_tag="ACG9CY_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS4936655.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45025..45753
                     /gene="sfsA"
                     /locus_tag="ACG9CY_000107"
     CDS             45025..45753
                     /gene="sfsA"
                     /locus_tag="ACG9CY_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS4936656.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45769..46782
                     /locus_tag="ACG9CY_000108"
     CDS             45769..46782
                     /locus_tag="ACG9CY_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4936657.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46782..48059
                     /locus_tag="ACG9CY_000109"
     CDS             46782..48059
                     /locus_tag="ACG9CY_000109"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4936658.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48122..50824
                     /locus_tag="ACG9CY_000110"
     CDS             48122..50824
                     /locus_tag="ACG9CY_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4936659.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50976..51293
                     /locus_tag="ACG9CY_000111"
     CDS             50976..51293
                     /locus_tag="ACG9CY_000111"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4936660.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51323..51643
                     /locus_tag="ACG9CY_000112"
     CDS             51323..51643
                     /locus_tag="ACG9CY_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4936661.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51751..53211
                     /locus_tag="ACG9CY_000113"
     CDS             51751..53211
                     /locus_tag="ACG9CY_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4936662.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53279..53500
                     /locus_tag="ACG9CY_000114"
     CDS             53279..53500
                     /locus_tag="ACG9CY_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936663.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53531..53713
                     /locus_tag="ACG9CY_000115"
     CDS             53531..53713
                     /locus_tag="ACG9CY_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS4936664.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53713..54126
                     /locus_tag="ACG9CY_000116"
     CDS             53713..54126
                     /locus_tag="ACG9CY_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS4936665.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54249..55430
                     /locus_tag="ACG9CY_000117"
     CDS             54249..55430
                     /locus_tag="ACG9CY_000117"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4936666.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55501..55665)
                     /locus_tag="ACG9CY_000118"
     CDS             complement(55501..55665)
                     /locus_tag="ACG9CY_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936667.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55819..55892
                     /locus_tag="ACG9CY_000119"
     tRNA            55819..55892
                     /locus_tag="ACG9CY_000119"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55853..55855,aa:Arg,seq:cct)
     gene            complement(55961..57100)
                     /locus_tag="ACG9CY_000120"
     CDS             complement(55961..57100)
                     /locus_tag="ACG9CY_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4936668.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            57272..57406
                     /locus_tag="ACG9CY_000121"
     CDS             57272..57406
                     /locus_tag="ACG9CY_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936669.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(57399..58034)
                     /locus_tag="ACG9CY_000122"
     CDS             complement(57399..58034)
                     /locus_tag="ACG9CY_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936670.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58147..58782)
                     /locus_tag="ACG9CY_000123"
     CDS             complement(58147..58782)
                     /locus_tag="ACG9CY_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFS4936671.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58816..59277)
                     /locus_tag="ACG9CY_000124"
     CDS             complement(58816..59277)
                     /locus_tag="ACG9CY_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFS4936672.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT        19463 a        10015 c        12558 g        17310 t
ORIGIN      
        1 gctataattt gaaaaaattt atcgttatta tatgattaag aaggattgaa taaagaaaga
       61 agttgaaaac gatacctgat tacaaaaaaa taacttttat aagttctata acgttgatta
      121 tatttttaat tcttattctt ctcgtcgtct tggttccccg aatttttggt ttgactcctt
      181 atattattga ggacaaagaa atgagtccat attatgcaca aggaaatctg atatatgtac
      241 gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat gaaaattcag
      301 gaaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa gatttttata
      361 caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga aatattatcg
      421 gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg gcatttgcat
      481 ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca gcatggaata
      541 cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa gaatgttaaa
      601 aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg agaaaacact
      661 catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag gagtagtatg
      721 cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt tatgcgtacc
      781 cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca tgtagcagaa
      841 gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg acagcttcag
      901 cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt catggccacg
      961 acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa
     1021 gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct tgaaggtcaa
     1081 gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat ggatagtgca
     1141 aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt
     1201 ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga agagattccc
     1261 cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat
     1321 cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat gacaaaggaa
     1381 tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc gattgaatcg
     1441 agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt aaaggataca
     1501 ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt caattgataa
     1561 ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta gttgggccaa
     1621 aataattcat tatttgctaa ttaaataact atttcgtatt gacagaagca atttaattat
     1681 ctataattat tgatgttccg ctgattcttg tcatagtagc tcagctggat agagcatccg
     1741 ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa accgttagaa
     1801 actgaggttt ctaacggttt ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc
     1861 aaataaaata aaaaaacatc cttatcgagt acaattataa ggagcaaaaa aataaaactc
     1921 atcagaaaag aggaagaaga tatgacgata ctatctagaa ttttagtgac tctagttgca
     1981 attgaatttt tttatatcat gtatatcgaa acggtgagaa ctgattctga cacgacaagt
     2041 agggttttta aaatgagtaa agaagagtta agcagaaaat ctgttcaaac attgtttaag
     2101 aaccaaggag tgtacaatgg actattagga gtgggactac tttatggagc ttatctttct
     2161 tccgcatcta aagaaatcac aagtatgcta ttaatatcta ttttttttgt tgcattatat
     2221 ggcagtttga ctagcgatcg ttggatttta ctcaaacaag gtgggctagc tctgttggca
     2281 ttgctttcta ttttcttata aatatagaaa aaataagaat ttatctaaca ggaaactttg
     2341 tgaaaaatat tactttatct catgaaatat gatattctaa tgaatggtat tttagaaaat
     2401 ttcatgaaaa aggagttaga taggtgttca aaaattatgt ttcctcacta aaaaaagaat
     2461 tttcaggtta tgatctttca agatttcaga aagatctttt ggctgggatc acagtagctg
     2521 ctgtagcatt gcctttagcc ttggcttttg gggtatcaag cggagctgat gcggccgcag
     2581 gtttgattac ggctatcatt gccggtatcg ttattggcgg tttatcaggc ggtttttatc
     2641 aaatatcagg tccgacaggt gcgatggcag ctatcttgat gtctattgcg gcaacaaagg
     2701 gaatgcaagg tgtattgctt gctacttttt tagcgggtct atttttactt gcagcaggac
     2761 ttttccgatt aggaacactg acttctttta tcccagcacc cgtaattacg gggtttactt
     2821 caggtattgc tatcattatt gcattaggtc agatcgataa tttatttggt gttcactcgg
     2881 aaggtacaaa cgttattgaa aaactaagta gttatcaaac gataggattt ccgataaaca
     2941 cagcctcttt gatcatggga gggctagtca ttttagggat gtttatctat ccaaaaaaat
     3001 gggcgaaacg aatgcctagt tcgctgctgg ctatcatttt ggcaacagca gttatgctat
     3061 tgttccattt accagtagca actgtgggta agattccaca aacattgatc agtagtaatc
     3121 gtttgaatat gggagatttt agcttttctg ccttgcaaca agtggcagtt ccagctatta
     3181 gcattgcttt attaggaatg attgaaagct tgctatgtgg tgcttcggct ggccgaatgg
     3241 caaataagcc cttagatagc aatcaagaat taatggcgca aggaattgga aatctgttgc
     3301 ttcctttctt tggcgggatt cctgctactg cggccatcgc ccgtacaagc gtggccatca
     3361 aatctggtgc acaaactaga ttagctggta tgatacatgc agttgtctta ctgctctcca
     3421 tgcttgtttt tgcgccagtg atgtcacaga ttccaatgcc agcacttgca ggtgttttga
     3481 ttgtgacggc ttggcgtatg aatgaatggg aaacgatcaa ggagctattt actaataaat
     3541 attggtcagc agttctattg tttatcctta cgatgatttg tacagttatc tttgatttaa
     3601 gtattgctat cgtgatcggg gtgatcggcg gctgtgtatt ttttgttgtc aaaagcgctg
     3661 ctatcacgat ttcattagaa acgatcgatt ggcaaagaat ggggctgcca gaaacggaaa
     3721 agctgaataa ctggacagta gtgtatatta gtggtccact gttttttatg tcttcagaaa
     3781 aactcaaaaa taaattggaa gaactaaatc atcacgatgg tgtcattttc tccatgcgag
     3841 gcgtaccaag tattgatttg acggcacgtg cagtttttga tgaatttcaa gaaaaagcag
     3901 agcaaagagg acaaacgatt atctatactt ccttgcagcc agaagtagaa aaacaacttt
     3961 ctcacttgtg ggaacataca ttcacgcatc aacatcaaac agttgctcat gcgttgacag
     4021 ctttacatgc gaaatatact gcaattagcc attaaaacaa tgaaaaagaa cagacaagac
     4081 tgtaaactga agattttatg gtcttgtctg ttctttttaa tattgctgta aaagtgaagc
     4141 tagtacttgc cagtcttgat ggataacagg tacaagggag cgattgtaga aaatagctgc
     4201 tggatgaaaa agtggaagaa ttcgatagtt tttttctgac cattcgtagg catccttttg
     4261 ttctgttaat tgtaagacag gccctgtata gagttgacca tgatttttgg taaccgtata
     4321 ttctttgcca agtaaacgtt gaagaccaat gtttccaagt gtcgcaatta attttggctg
     4381 gaccttcatc agttcgtaat ccaatattgg tgcatgcgca aatacttcgg aacgagtagg
     4441 tgtccgattt ggataaacag tttctgactg ttgtgtccgt ttattgatcc gatgagtcac
     4501 gcgatacggg cggctcctta cagcactggt gatatacacc tcttctcgtg ttaagccagt
     4561 tgaagaaagt gcctgcatca attcttttcc agcttgaccg ctaaaaggaa catgcgtatc
     4621 aatctctgtt ttccctgggg cttctcccac gagcatcagt ttaggatgaa gaggtccttg
     4681 cccagcgata aatccttcta caggacggcc gttcattctt aaagaagctt cttgtatcag
     4741 ttcttttggg tattccattt atgctcctcc tttttctttt attataagaa taaaagaaac
     4801 acaatgcgac tattttattt ggtagaactg tgaggataga aaagcagaag tattgcttga
     4861 atttttgttt tactttttga ttttaaattt agttgactaa cggttgctgt tctggtaagc
     4921 tagtactgtt gtaattgtgg actccacagc tacaaccgct cagaacaagt aataagcaca
     4981 gcatatgctg tcacttcgga tggcgagtct aagtatatca gaaggaggtg ctttatagca
     5041 tgtacgcaat tattaaaact ggtggtaaac aagtaaaagt tgaagtggga caagcaatct
     5101 atgttgaaaa acttgacgtt gaagcaggag aaaaagttgt ttttgacgaa gttatcttag
     5161 taggcggaga atctacaaaa gtaggcgctc caactgttgc aggtgcaact gttgaaggta
     5221 ctgttgaaaa acacggcaaa caaaagaaag tcgttacttt caaatacaaa cctaaaaaac
     5281 attcacaccg taaacaaggt catcgtcaac catatacaaa agtggttatc gacgcgatca
     5341 acgcttaata gcgtttgaga aagaaggcct atatatgatc aaaggaacat ttaagcgaaa
     5401 tgacgcaggt cagatcgttt cattcacttt gaccgggcat gctgatgctg gtccttatgg
     5461 aagtgatatc gtttgtgcgg gtgtttctgc gttagcgatc agcactgtca acgggattgc
     5521 ctcattagcc ggttttgagc cgattgttga aatgaatgaa gaagaaggcg gttatcttta
     5581 taccgaagtc acttcaggaa tgacacaaga acaaaacaat attgctgaga ttctattaga
     5641 aaatctttta ttaggattac aatcgataga agctgaaaac tcagaatata tccaaattaa
     5701 aacaattaat gataaatagg aggtgcagac catgttgtta tctatgaatc tacaattatt
     5761 tgcccacaaa aaaggtggcg gttctacatc taacggccgt gactcagaat caaaacgtct
     5821 aggtgctaaa cgtgctgatg gacaaacagt tacaggtgga tcaattcttt accgtcaacg
     5881 cggtacaaaa atctatccag gtgtaaacgt gggtatcggt ggagacgaca ctttatttgc
     5941 aaaagttgat ggcgtagtac gtttcgaacg taaaggccgt aacaaaaaac aagtgtctgt
     6001 ttatccagta gctcaagaag cttaattgaa tcttatgatt aaagaggttg tgacaagact
     6061 tttgtcacag ccttttttgt ttataaaaat aaaagttgta gtggaggcta cgaagtttac
     6121 tttgtctttt tacacttaat atttttttgc caatttggaa tcaggatgtt ttgataacta
     6181 tcgttttcaa ttgatggaag ctatattgtt tgctatcagt gaaataatta tatggtaaac
     6241 agtgttcttt ttcttgtttc cattataaaa atcgtatcgg gatttttcgt tcttttaaat
     6301 tgaacatttc tatctatccg cgtgttatca acaaaacaaa atcaggtaaa tagttgacga
     6361 aacagaaata aatacatagt attatgtttt gaagcctaag tacaatagtc tatagaaaag
     6421 gagcgatacc attggcaaaa cgcattgacc gaatttatat gtatgtcaaa gaaaaaaccg
     6481 ctcatttaac aaaggcagaa tatgatcaag gagtaacaac acaggaaatt gctgaagtgt
     6541 tagggattca gcgaacaaat agcagtaaag atctgaatca gctagtgcgc gaaggaaaac
     6601 tgataaagac agacggacga ccagtacgtt acatatatca aacactcgtt acgcacgtaa
     6661 agccagttac aaaacatgta gtcagttaca aagaaacgcc ggtctctgaa gaaaaaaata
     6721 cactccaaat agatactaag gatatctttg caaaaatcat tggagctaac ggcagcatga
     6781 aaaattcagt ggaacaggca aaagctgcga ttttgtatcc gccaagagga ttaaattgtt
     6841 tgattacagg acctaccgga tcaggaaaga cttattttgc acacgcgatg tatcattttg
     6901 caaaagcgca tcatgtaata gatgaggaaa cggaactgat tgttttcaat tgtgctgatt
     6961 atgccaataa ccctgagctt ttgatgagtc atttatttgg gtacgcaaaa ggtgctttta
     7021 caggcgcaga tgaagaaaaa atggggatta tcgatcaagc ggatggcggt atgctgtttt
     7081 tggatgagat tcaccggttg ccgcctgaag gtcaagaaat gatcttttac tttatggatc
     7141 atggaactta cagtcgtctt ggggaaacaa cgaaatccca tgaagcaaat gtacggatcg
     7201 taggtgcgac cacagaagac ccaggttctt ctctattaga aacttttgtt cggcgtatcc
     7261 caatcaatat caaacttccg gcttttgaaa aacgtcctgc aaatgagaaa gtcgatttag
     7321 taaaaatcat gattgcccac gaagcaaacc gcacgcagcg aaagatttct ttgacagaag
     7381 atgtagcaaa agcattgatc ggcagtgtga cctatggaaa tataggacaa ttgaagtcaa
     7441 atatccagtt agtatgtgca cgaggatttc taaatcatat gcaatcaccc gaaatctcga
     7501 tcacaatcga tgatttgaca gaagggattc gtagcggatt gattcaatta gcaagcaacc
     7561 gcagtgcaat ggcagagctt tctaaaatat tggagccaaa gataactgta gcaccaaatg
     7621 atacgatcat gaaaatccaa tcggactcct atgagttacc ttataatcta tatgatatca
     7681 tcggagataa agcagcttta ctgaagtcgg atggtttgga tcaagaagca atcaatcatt
     7741 tcatttctac agatatcaac atccatctga agtctttcta taaagaccat ggtttttctt
     7801 ttaatgcaga cagcaaatta gcagaatttg tagatccgaa aatcatagaa gtgaccaatc
     7861 agatttatgt aatggtcaaa agatctttac catatgagtt tcagcaaaac tttatttatg
     7921 ctatgagctt gcatatcagt tcttttttga aaaaaatcaa cttaggagaa gaacggcata
     7981 cgaatgataa catccgtgag atggcttttg attttccaga tgaatatgct gttgcgaaag
     8041 aagtgcgtag ttatatcgaa acgtattttc aagtggagat tccagaaagt gaagactatt
     8101 acttgaccgt tctactcgtc tcgttacggg caaatcaagc aagcggcaga atcggtgtag
     8161 tcgttgcagc tcacggaaac agcacagcaa gcagtatggt ccaagttgtc caacagttgt
     8221 tagatgcaga gaacgtacga gcagtagaca tgcctttgga tatggatccg aaaacagcat
     8281 tggaccgaat tgaacgcaat gtacaagaag tagatgaagg aagcggggtc atactcttag
     8341 tagatatggg gtcccttgcg tcgtttaatt cgcaaattca aagagacact ggaattccag
     8401 tacgtactgt tgatatggta acaacttctc tagttttgga aactgtacga aaggtctcag
     8461 tattagggac agatctagat atgttgcatg aatcattgaa aaattttcga ggttacgcag
     8521 aaatatcttc tgtagaatct gttcaggaag caaaacctat tcataagaaa aaagctgttt
     8581 tggctatctg cgcatctggc gaaggtacag cacaaagaat caaagaattg atcgaacgag
     8641 cggtaaacaa gcgtcaagaa acagaattag aagttctagc tttgtcgatt gtggaagtga
     8701 aagaagaact gccagttatc caaaaagaat atcagatcat agctactact gggattactg
     8761 atttgaatat ccaagcccca tttattcctt tagaacgttt tattgatcaa aatatcgaag
     8821 tcattttgga tcagttgctg atggaatcag aattggagga aactgaattc atctccctag
     8881 atgaagagtc agctaaaaat acgtgtgttg agtttatctc tgataacttc atctttatta
     8941 atggtagtaa gttaatcgat ccaatgtggc aattttctac acaaatctct cagacgacgg
     9001 gaatcggcga tgaagaatat ggattcaaaa tcaacctagt gatgcacaca gcaggaatga
     9061 tcgaacgtat tattcggaat gaaccattga ctgtggaaga aaacgaattg acaaatacaa
     9121 cgaatgatcc tctttatagc caattggcag cctcagttgt tctgttggaa gatcagataa
     9181 aagtaaaagt gccgatagaa gaaatgtatt atctgctacg tctagtccat aatcagttag
     9241 ataaaaaaga atacactgtg ccatgaatac acaaaaacga tacactttta tagtgtatcg
     9301 tttttgtgta ttggttaaga atcctttaaa ttcaattagt gtatcgtaaa aaagttggca
     9361 cgctttttgc ttatagtagt gtgtgctagg aggtggttgt tcacacagaa atgatagggg
     9421 tttctgtgaa gtagtgagga agaaggagtt ttgcagatgg atattcgatt ggcacgtata
     9481 gatgatcgtt taatccacgg gcaagtcgca acagtttggt cgaagcaaac aggtgttgaa
     9541 cgtatcattg tgatcagcga tgtagtcgct caagataaat tgagaaagtt tctactaaaa
     9601 gaagctgcac cgccggggat cacagcaaat gtaataaccg tgcagaaaat gattcaaatc
     9661 tatcatagtg gattattatc acgagcaaaa gtgatgcttt tattcaccaa tccccaagat
     9721 gttgaagcgg ttgtccgctg cggtgtacag ttgaagtcgt tgaatatcgg cggtatgagt
     9781 tttactgaag gcaaatcaat gattaccaat tttgtttcag taaatcaaca agatattgat
     9841 tcctttcgtt acttgaacag tcaagaagtg gagttagaga ttcgaaaagt acctgctgac
     9901 aggaaaatcc ttttgatgga actactagcg aaaatcaaaa aggtgtagca agaatggttg
     9961 ttggaaaagg tcaaataatt ctgttgtaat ggttagcaat tattaatatt tttgaatttg
    10021 ttacaacaaa agtaaatagg aggtataaat atggtaggga ttatcctagc aagtcatgga
    10081 caatttgctg aaggaatctt acaatccggt tcaatgatct ttggcgaaca agaaaacgtc
    10141 aaagccgtga ttttgaaacc aagtgaaggt ccagatgact tgagagccaa attggaagaa
    10201 gcggttgctt cttttgataa tcaagacgaa gtgttattct tagttgattt atggggcgga
    10261 acaccgttca atcaatcaaa cacattattc gaagagcaca aagataaatg ggcaatcgtc
    10321 agcggtttga atttaccaat gttgattgaa gcctacgcat cacgcttttc aatggaatct
    10381 gctcatgaaa ttgcagcaca catcattgaa acagcaaaag acggtgtaaa agtaaaaccg
    10441 gaagaattag aaccggcaga agcaccgaaa gcagcagttg aagacgcaca acctaaagga
    10501 gcacttccag aaggaactgt ggtcggagac ggaaaaatca aatatgtatt agcacgtgtt
    10561 gactcacgtc tgttacatgg acaagttgcg acagcttgga caaaagcagt gcaaccaaac
    10621 cgtattatcg ttgtttcaga tgcagtatca aaggatgatc tgcgtaagag actaatcgaa
    10681 caagctgctc ctccaggtgt aaaagcaaat gttattccta ttagcaaaat gatcgaagta
    10741 gcaaaagacc ctcgttttgg aaataccaaa gcattgcttt tatttgaaaa tcctgaagac
    10801 gtattgacag ctgttgaagg tggcgtagac atcaaagaat taaatgttgg gtcaatggcg
    10861 cattcggtcg gtaaagtcgt tgtaagtaaa gtattgtcta tgggacaaga agacgttgaa
    10921 gcgtttgaga agcttgaaca aaaaggtgtg aaattcgatg tacgtaaagt acctaatgat
    10981 tcacgcgata acatggatga tattttgaaa aaagcaaaag ctgaattagc aaaagcgtaa
    11041 taaataatat taaaatttat aggaggctta tcatgtctat tatttcaata attttagtcg
    11101 tattagttgc ctttctagct ggtatggaag gtattcttga tgaatttcaa tttcatcaac
    11161 cactagtggc atgtacattg atcggtttag ttaccggtaa cctcgaagct ggtatcgtac
    11221 ttggcggtac gcttcagatg atcgctttag gttgggcaaa tatcggtgcc gctgtagcac
    11281 cagatgctgc attagcatca gtagcatcag caattatttt agttttaggt ggacaaggtg
    11341 ttaaaggtgt tccttctgcg atcgcgattg ccgttccttt ggctgttgca gggttgttct
    11401 taacaatgat cgtacgtaca atcgctgttc caatcgttca tttgatggat gcggctgctg
    11461 aagaaggaaa catccgtaag gttgaaatgt ggcacatcat tgctgtatgt ttacaagggg
    11521 ttcgtatcgc ggtcccagca gcagcgttgc tatttatccc agcacaaact gtgcaatcat
    11581 tccttgaatc aatgccagca tggttaacag atggtatggc tatcggtggg ggaatggtcg
    11641 tagctgtagg ttacgctttg gtaatcaaca tgatggctac aaaagaagta tggccattct
    11701 tcgttattgg tttcgttgta gctgcaattt ctcaattaac attgattgct ctaggtgctt
    11761 taggtgttgc attagctctt atttacttga acctttctaa aatgggtggt tcatcaaata
    11821 atggaggcgg aagcaattct ggtgaccctt taggcgacat tttgaatgac tattaatctt
    11881 gaaggaggaa aataaaatgg cagaagaaaa aatcaaatta tcaaaaaaag atcgtttagc
    11941 tgttgcgtgg cgctctacct tcattcaagg ttcatggaac tacgaacgta tgcaaaacgg
    12001 tggctgggca ttttctatga ttccagcaat caaaaaatta tacaaaacaa aagaagaacg
    12061 ttctgcagca ttaaaacgtc acttggaatt ctttaataca cacccataca ttgcttctcc
    12121 aattttaggg gttacattgg ctctggaaga agaacgtgcg aatggcgcgc ctgttgatga
    12181 tgtagcgatt caaggggtta aagtaggtat gatgggtcct ttagctggtg ttggtgaccc
    12241 agtgttctgg tttacagtac gtccaatgct aggtgcacta ggtgcttcat tagctatggg
    12301 aggaaatatc cttggaccaa tcatcttctt cttaggttgg aacattattc gttgggcatt
    12361 catgtggtat acacaagaat ttggctacaa agctggttct aaaattactg atgacctttc
    12421 tggcggattg ttacaagacg taacaaaagg tgcttcaatt ttagggatgt tcgtattagc
    12481 tgcccttgta caaagatggg tatcaatcaa attcttacca gttgtatcaa ctgtaaaatt
    12541 agataaaggt gcttacatcg aatgggataa attgccttca ggtggtgaag gaatcaagag
    12601 tgcatttgaa caagttaata atggcttagc cctttctcct gaaaaggtaa caacattaca
    12661 agacaactta gaccaattga ttcctggttt agctgcatta ggattaacat tgttatgtat
    12721 gtggttgctt aagaaaaagg tttctccaat cgttatcatc cttggtttat tcgtagtcgg
    12781 tgttgttgga cacgtcatcg gacttttata atccataaaa atatagctcg ggcgttgtcc
    12841 cgagcttttt ttatatagta tactgttcat aaacaaatat ctggagggag aaaatggttc
    12901 aatcaatcaa tacaaaagta gacttggtaa ttgatgctac ttcgtttacg ggtctgacag
    12961 attacgggca aatcatgata ggtaataatg gttttgagtt ttaccatgcg cgagatagac
    13021 gcaaatatat ccaaattcca tgggaagaag tggattatgt actagcatca gtcatgctaa
    13081 agggaaaatg gattcctcga tatgccattc aaacgaaaaa aaatggtaca tacacatttt
    13141 cttccaaaga acctaaaaaa gtgcttcgtg cgatcagaga atatgtagat gaagatagaa
    13201 tggtacaatc attgagtttc tttgatgtag tgaaacgtgg tattcgtagt atgtttaaac
    13261 gcaagtaaaa aataaagaaa acttctaaga ggaggttttc tttatttttt tactttgcag
    13321 aagaaaatat gaaatagcaa aaccaattat ttgtcccctt taaatcccat atgctaaaat
    13381 tgattagcaa agaaggaggt tatattatga gttggttatg gacattgatt gttggagccg
    13441 tgatcggagc aattgcaggt gcgatcacca gtaaaggaaa ttcgatgggc tgtgtaacga
    13501 atatcattgc tggactagtc ggttcaagta ttggacaagc gattttcggc gaatggggac
    13561 caaaagctgc aggtatggca cttattccat ctattttagg cgcagtgatt ttggtagcag
    13621 ttgtctcatt tttctttgga aaacgatcat aagtttttac aaaatagtaa actaaaagga
    13681 gttgcagcga gactgtgact cctttttggc tttatttatt taaaataaaa agaagttttc
    13741 cggaaatttt tatttaactg tcgtgtacga agaaaagttt gctataatgg aaaaaagcgt
    13801 gcaaagaaag agggagtttc attatgatgc tacgtgtaga aaaattgcgc aaaaaaatgc
    13861 aagaagaaaa tttggattca tttttggtta ccagtccgta caatcttcgt tacttaacga
    13921 actttacagg tacgacagga ttagctgtta ttactttgga aaaagcattt tttattactg
    13981 attttcgtta taccgagcag gcggcagcgc aggctcaagg gtttgaaatt attaaaaatg
    14041 tcggacctat ttttgatgag gtagcaaact tagtacgcaa agaagaactg cactcattag
    14101 cttttgagga aacaacagtc tcatttttag aatattcggt tttagaagaa atcatcgatg
    14161 cggaattagt acctgtttca cagatgatcg aagaactcag agaagtgaaa gatgaagaag
    14221 aaatcgctat tatcgaaaaa gcatgtcata ttgcggatat ggcatatgat cacatcctga
    14281 aaatgatcca accgggcatg actgagattg aagtcgcaaa tcaactagac ttttatatgc
    14341 gttctcttgg cgcttcaggt gtttcttttg atacgatcgt tgctagcgga ttgcgttcgg
    14401 ctatgccgca tggtgtagca agcaaaaaaa tcatcgaaca aggtgacttg atcacattgg
    14461 actttggttg ctattacgaa gggtacgttt ccgatatgac acgtacattc gctattggtg
    14521 atcctgggga aaaattaaaa gaaatttatc aaatagtctt agatgctcaa ttagcagtta
    14581 ttgatgctgc aaaaccagga atgactggtg tccaattaga tgcagttgct agagattata
    14641 tttcaaaaca tggttacggg gaagcatttg gacattctac aggacatggg atcggtttag
    14701 agatccacga aggaccaaac gtttcttctc gtgcagaaaa gcaatttgta ccaggaaatg
    14761 tgatcactga tgaaccaggg atctacttac caggaatcgg tggagtacgt attgaggatg
    14821 acttgttgat cacagaagat ggaaatcgtg tgttgactcg ttcaccaaaa gaattgatca
    14881 tcttatagtt tcatcaatag aagaagtaag cgtaaaaatc agtgttcaca gactttgtta
    14941 tagaaaatca ctcgatttaa tgatacacta tagaagaact tttaagctgt aagagataat
    15001 aaagttgggt gttttctcca attcatagca gtttgcagct gatcggcggc agttttctta
    15061 ccacgtgatc actatgccgc atcatagaga attcggatgt atctcgtgct taggcaaagg
    15121 gaggaataaa taaatggcag atgaaaaaaa tttgatttta aacgcaaacc aagatttagg
    15181 agagatcgtg atcgctcctg aagtcatcga agtaattatc gggattgcag cttctaaagt
    15241 agaaggcgta tacggaatgc gcggaacttt tgccagcaat gtcactgaat tattaggacg
    15301 tgcagctcac ggaaaaggtg tttacttagc aaacgaggaa gatggcttga aagtagatct
    15361 ttactgttat ctagaatatg gtgtttcagt tcctaaagta gcaatggaaa tgcaggaacg
    15421 ggtcagccag caagtccttt tcatgacgga tatcgattta tctgaagtca atatccatgt
    15481 agtagctgtt gttcctgaaa aattagaaca accaacaatc gaagaacttt ttgaagacga
    15541 agaggaagag aatgaataaa gaattatcaa gacacgagat tcgggaaatg gcattgcagg
    15601 ctttatttcc attagacttc aatgcagacc taacaaaaga agatgcgatt ttcaacgcaa
    15661 tcgaattaga ccaccgcgac atgatcaacg aagatgaatc agagtttgtt cctgtttatc
    15721 ttgatacgct agttggcggt gtctgtgcaa aaaaagatga attggataaa gtgattgaaa
    15781 aacatttaaa aaacaattgg cacatcaatc ggatctcaaa aatggatttg gtgatcttgc
    15841 gtatcgcgat ttttgaaatg acttatgtga cagatgtacc agcggctgtt gctttaaatg
    15901 aagcaatcga gttggcaaaa acatttagtg atgaccgttc acgtaaattc gtcaacggtg
    15961 ttttatccaa tgtattgaaa gaattagaag ctggggcgta agtcctagct tttttctatg
    16021 aatcatcata aacctgttat aaatcgggaa aattctatgg tagaatgaaa acacaaaaaa
    16081 tatctgggag tggttagatg gcagaattga ttaacggaaa agaattagct gaaaaaatgc
    16141 aggcagagat tgctgaaaaa atccaaaaat tgaaagataa tggtatccat cccggattag
    16201 tcgttttatt ggtaggggaa aatccagcca gccaaatcta tgtaagaaat aaagaacgtg
    16261 tagcaaaaga aatcggaatc cattcattgg tagagcgtta tcctgagacg atttctgaag
    16321 acgaactact tgcagaaatt gaaaaataca atcaagatcc tagttttcat ggtatattgg
    16381 tccaattacc attgccaaag catatagatg aagaaaagat cctgttagct atccgccctg
    16441 aaaaagacgt agacggtttt catccgatga atttaggcag actccttgcg ggtaaaccgg
    16501 acaagattcc ttgtactcca tacggcatca tgaaaatgtt tgaagcttat tctatcgatc
    16561 cggcgggtaa acgagcagta gttatcggac gaagcaatat cgtcggtaaa ccgatggctc
    16621 agctactctt gatggcagat gcaactgtta ctgttgctca ttctaaaacc gaaaatctgg
    16681 ctgaactggc acaagaagca gatattttag tcgtagcaat aggacgtgga cattttgtca
    16741 caaaagaatt cgtaaaacca ggtgctgtag tgatagatgt agggatgaat cgtgatgcta
    16801 acggtaagct gatcggtgat gtgaaatttg atgaagtgga gcctattgca ggctggatca
    16861 ctcctgtgcc aaagggtgta gggccaatga ctatcacgat gctgatgtat caaaccgtaa
    16921 aatctgccga agcttttgga gcaggtgaat agatggcaca agaatattta actgtcacta
    16981 cattaacgaa gtatttgaaa agaaaatttg atgccgatcc ttatttagaa cgcgtatatt
    17041 taacaggcga aatatcgaat ttccgtttac gtgccaatgc ccatcaatat ttcagcttaa
    17101 aagatgacca tgcaaaaata tctgctatta tgttcaaagg agcattccaa aaattacgat
    17161 tccagccaaa agaaggaatg aaagtccttg tcgttgggcg tatttcatta tatgaagcgg
    17221 gaggttctta tcaaatctat gtagagcaca tggagcctga tggaataggt gctttatatc
    17281 aagaattgga agaacgaaaa gaaaagctga tgaaggaagg cctttttcaa gggcctaaaa
    17341 aaatgctgcc acgtttccca aagcgaatcg cggtccttac cagtccaagc ggagcggtta
    17401 tccgcgatat catcacgacg gtgaaaagaa gatatccaat tgtccaattg gttttgttcc
    17461 caacagttgt acaaggaaac caagcagcgg acaatgttgt caggaatatc cagagagtag
    17521 aagagatggg caatttcgat acaatgatca tcggtcgtgg gggtggttcg attgaggatt
    17581 tatggccgtt taatgaagag cgggttgctc gggcgattta tcaagctaat acgccggtga
    17641 tttcatctgt aggacatgaa acagatacca cgatcgctga tttagtagca gatgttcggg
    17701 ctgccactcc aacagcagct gcagaattag ctgttcctgt tttgtcagaa gagttgatgc
    17761 gcattcaaga acggcaagct agattgcagc aggcgtttgt acgccagatc cagcgcaaac
    17821 aagaacggtt cgagcgtgct cgacgttcct atgtatttcg gcagcctgaa agattgtatg
    17881 aaggtcagac catcaaatta gaccagttga aacagcgatt atatcaagct acacagcaaa
    17941 tttatcatgc gaaagaaaaa caaacgatcg cattaagcca ccaattggaa caagtggctc
    18001 cgctttatcg agttaaagca gctcgccaag aaactgctta tctagaaaaa cgcttagaag
    18061 agaaaatcca acaatatatg aaacaacagc agcagcgttt tgctcaagct gttcaatctc
    18121 ttgatttgtt gagtccattg aaaatcatgg gaagaggcta cagctatacg acgttaaatg
    18181 atcaagtagt aaaaagtgtc accgagattc atcaagggga tttgctgacg atccattatc
    18241 aagatggaat cgttaaagga caagtacaag agatagacaa aggagaataa aaatatggaa
    18301 aatccaactt ttgaagagtc actgcaagaa ttagaaaaaa ttgtcatgca gttagaacaa
    18361 ggagacgttc cattagaatc agctctagat tcgtttaaac gaggaatgga attaagcaaa
    18421 cattgccaag atacattagc taaagcagaa gagaccttga cgaaaatgat gacagaatca
    18481 aatgaagaag ttgattttga cggaaacgag gaaagctgag atgagattaa ctgacttttc
    18541 agctattcat ctacctgctg tagaaaaaga aatcttgtct tttttagatg agcatacaac
    18601 ggatagagga ctctatgatg cgatgagtta ttcagtgaaa gcaggcggga aaagaatacg
    18661 tcccttatta cttttaacgg ccgttgcttc ctttgatgag cctattgatg ttcccgtcta
    18721 tcaagtagca gctgctttag aaatggtgca tacgtattcc ttgatccacg atgatttgcc
    18781 tgcgatggac aatgatgatc ttcgtagagg aaaacctacc aatcataaag tattcggcga
    18841 agcattagct atattggccg gagatggttt gcttacagga gcattccagc taatcagtat
    18901 ggcacactta ggtaattctc ctaagcttct gctgcttcag caattagctg tttgtgcggg
    18961 atcacaaggt atggttgctg ggcaagctgc agatatcgag ggcgaatcga aaaagctttc
    19021 tttagaagaa ttagcattta ttcatgaaag aaagacaggg catttgattc gttatgcctt
    19081 gctggctggt ggcatattag cgaagcaacc tgaagaaatc ttattgctac ttcaacgatt
    19141 agccgaacat ttaggtttag cttttcagat aagagatgat ctactcgatg tgattggtac
    19201 aacgaaaaca ttagggaaaa cagctggtaa agatgaacgt atggaaaaaa atacttaccc
    19261 gcgtttatta ggcctagaaa agacacgtga ggcgttggag attgagctgt tatccgccaa
    19321 caagatcata gacaaattag aagaggacgt tttttctttt gatggcgggc tgatgcgcca
    19381 gatgatcaag caatttgatg tggagaaatg agggaaagaa tggaaaaaga acgcgtagat
    19441 gtattagcgg tcaaacaagg attatttgaa acaagagaac aagccaaacg ttcagtgatg
    19501 gctggtttga tttacaacga aaaaaacgag cggtttgata aaccgggaga aaaaattcca
    19561 gtgagtagtg aattaagagt caaaggtaaa aaacttcctt atgtttctcg agggggatta
    19621 aaattagaaa aagcactcaa agcatttgat ctatctgttg aaggaaaaac attgttagat
    19681 attggtgcat ccactggcgg atttactgat gcagctttgc aaaatggcgc aaaaatgagt
    19741 tatgctttag acgttggcta caatcagcta gcttggaaaa taagacaaga tcctagagtc
    19801 gtcgtaatgg aaagagtgaa ttttcgttat gcaaaacccg aagactttac cttaggaatt
    19861 ccagaagttg ctgttatcga tgtttctttt atttcactaa aactgatgct gcctccatta
    19921 catgctattt taaaagaaga gggagaagtg attgctttaa tcaaacccca attcgaagca
    19981 ggcagagaag ctgtagggaa aaacggaatt gttcgtgacc ctcagaccca tcaaaaagtt
    20041 ttagaagaca ttttgtcatt tgcggcacaa ggtggatatg atgtgctgga attaagttat
    20101 tctccgatca cggggggaga aggaaatatc gagttcttgg ctcacttgag gaaagtaccc
    20161 gaaagcggaa caatcaattc agctattaat atggcagaag tggtttcaaa tgcacacgaa
    20221 cagtttgacc ataaataaat tgtaactgtg aaaatcaagt gagaagtatg cggattcttc
    20281 tttattatac caaggaaata agctatcatt gattaagaag aatatgctaa ggagcgaaga
    20341 cgtaatgaga aaaaaagatc ggcatcgttt gattactcga ttgttgagtg agcaagatat
    20401 ccgaaagcag gaagaatttg ttgaaatcct gaaaagcaaa ggaatatctg ttactcaagc
    20461 tacgatttcc agagatataa aggaactgaa actgatcaag gtaccggctt cagatggcgg
    20521 ctaccgttat agcataccag ctgaaacaaa cgaagatgta aatactaaat tggaaaaact
    20581 attaaaagac gctttcgtgg cagttgacca aatggaaaaa tttgttattt tgaaaacatt
    20641 gccgggaaat gcttcggcag cagcaaattt gattgataaa cgttacaaga aagaattatt
    20701 ttcgattatc aatgatgacg ataatgtttt gatgattaca cgaactgaag accaggcagt
    20761 tttattgaag aaagacttcc tgcattacct ttaagttgag gagcgaatgt gatgctacaa
    20821 gaaatcagta ttactaactt tgcgattatt ccggaattgc ggctttcttt tcacgaagga
    20881 atgaccgcgc tgaccggaga gactggtgcg ggtaaatcca ttattatcga tgctttaggt
    20941 ctgttagcag gaggaagagg ttcaagcgat tatatacgtc aaggagcaga aaaatgtgtg
    21001 cttgaagggt tgtttgaact accaaaacaa gaaggattct ctgaactgat ggttgaatta
    21061 gggatcgaaa cagatgagga caatctgatt gttcgacggg atatgtcctt aactggaaaa
    21121 aatgtttgtc gagtgaatgg tcatattatc acattagcta atctgagaag aatcggcagt
    21181 tatttagtag atatccaagg acaaaacgaa catcaggaac tgctccagcc tgaatcacat
    21241 cttgctttat tggatcgttt tggagatgta gctttccaac agaagaaaaa gagttatcag
    21301 caagaatata tctcttatcg tgagctggaa aaaagggtac gaaaagtgca gcaaaatgaa
    21361 aaatcctatg ttcagagaat agatatgctc catttccagc aagaagaaat agctgctgct
    21421 gatttgcaag tcggagaaga agagaaatta aaagaagaaa gagaaaaact tagcaattat
    21481 caaaaaattg ctgatggatt agctgcaggt tacggagcac tgacgaataa tgaacaaaac
    21541 agtgtagatg gtgttggctt ggccgtttca gaactgcaag ggatcgccca tctagatgtg
    21601 gagtatgaag caatttatga aaatatccaa agtgcttact atttgctgca agatgcaatc
    21661 ggcgatatga gtcgtcagat tgatttattg gagttagatg agagtcgttt agaagaagtt
    21721 acgcaacgtt tagaattaat ccgacagttg aaacgtaaat acggcgaatc cattgagtca
    21781 atattggctt actatgacga aatcaccgaa gagctagctt cttctgattt ttcagaaggt
    21841 caattggaca aaatgaaaga ggaactagag caaaaagagc ttctacttca gcaacaagcc
    21901 gcagatcttc atgaagcgcg taaaaaaatc gcaaaagaat tggaaaaatc cattctgcat
    21961 gaattgaaaa gtctgtatat ggaaaacact gagtttgagg tacgtttctt aaaagaagaa
    22021 aatagacaat tgaatcatga tggatttgac caaatcgaat tttacattac gaccaatcca
    22081 ggagagccac tgaagccttt ggtgaaagtc gcttctggtg gcgaactgtc tcggatgttg
    22141 cttgctttga aaactatttt ttcttctgag caaggtgtga caagtattat ttttgatgaa
    22201 gtggatacag gtgtgagcgg aagagttgca caggccatcg cagataaaat ttctcaaata
    22261 tcgaaatact ctcaagtttt gtgcatcact catctgcctc aagttgctgc agtcgccgac
    22321 tatcaatatt atattgtgaa agcagtgatt gatggacgta cgcaaacttc tgtttcagag
    22381 ttgaaaacaa aagaaagaga agaagaaatt gcacgtatgc tggctggaag tgagattaca
    22441 aaactcacac tcgaacatgc aaaagaacta ttgaaactcg caaaaaggac ctgagccacc
    22501 tcgcagtggt tcaggtcctt ttcagcagct gatagctata ttttattttt acatgacact
    22561 cattacagca gtcaatacaa cagatccaat cgttaaaatc agcattgtcc aaatgacaaa
    22621 ttttgttact ttagcaaaag tacttgtttg ttttttttca cgcattgttt tcaacctcgt
    22681 cattaattag ttgattcaag tgtacaatct tttttccatt tccacaagag ggctttttta
    22741 taaaaacttc ccttttttta aataataggt agttacgata caaataaata atgttaatag
    22801 aactagccat ataaaggcat catccctttc agcaataggt aacttaatgt tcattccata
    22861 gatactgccg acgattgtcg gtatagttaa aataatcgtt aaagaagtta aaattttcat
    22921 aatattattc agattattcg atacgattgc tgaaaaagtc tgattcattt gttctactag
    22981 ttttaattga atgcgagaag tagtcattgc ctgctctgtt tccaccaata catcacgtaa
    23041 atgatttttc aaagcatgat tttcttggaa aagtttattt tgcgctagtt ctttcaatgc
    23101 atctaaatta gcagaagtcg cttctctgaa ataaacgagg cttttttgga tatccatgat
    23161 ttgataaagt tgtttgtttt cagtggatac ttgaagtttg gcttccaaag tatcgcattg
    23221 agattgaagc gtagctaaat aactgttgta agagagtgtg acctgccaaa tgaattgaaa
    23281 gaagatattg agattaggcg aaatatcact ttttggaaag gtctggctaa agactttctt
    23341 taaaaacagt ggttcatgat tcgtaattgt gataagcttc tttttgggcg tcaaaataat
    23401 agagagcggg tatgtatcca cttgaaaata accgctagga cttgttttag caaaaggata
    23461 ttgtaacaat aataacgctg gctttgtcaa ctttgattga ttgaattctt ctgtacggga
    23521 attttctgca tcgtccaata tggaagtgat gtaatctgac ggaatttcaa attgatgtgt
    23581 cagctgatga atctcttctt gattaggacg ttctacacaa taccagatcg tttggtcagt
    23641 ttcctttccg gtattacgca gtttctcatt atctaatgta aaatatttga tcatgatttt
    23701 cctcctgttg cctgtttctt ttattataac ggatagggaa ttggaataca tggaatgtgt
    23761 ttgtaagata tagaatataa ctgcataaat ttaaataaaa tgaaacagtt gtgtaaagta
    23821 tatacagtat acttgaagca acgataaaaa aggagtttac gttcatgggc acattcacac
    23881 cacttatctt gttttggtat atttacccgg taatcgtttt gtttggttgc caatttcttg
    23941 taaaattatt ttcgttaaat cgtcgtttta aaataaaagc ccctgatttg gcgattcctt
    24001 ttttgtgggg cggtatgcat gcattgtcta gaaatacagg gacaatttct atcttaccgt
    24061 ttttctttat ttctgttttg ctaatgggaa tttgtattgc tgttttccaa gcatattatt
    24121 atgaagaaat tatttatccg cgttatttca agatgacttg gcgtttggtt tttctgttga
    24181 caatcgtctt gtacgctgtc ttgatcattg tgaacatact ttcttaccta tgaaaaaaat
    24241 agaaaagctg taaaaagtct tttaaaaggc tttttgcagc ttgattttta aaaaaatgtt
    24301 tatgaataaa aaaagttgtt cccggatttc ccagaaaagg tggtagaaag tgggggattg
    24361 tggtagacta attttagtta gtggaggaat ggggggcttt caatgttgat gggtgaattc
    24421 caacataata ttgatgcaaa aggacgttta atcgtgccct caaaacttcg tgaagaactt
    24481 ggcgaaaagt ttgttctgac acgcggattg gatggctgtc ttttcggcta tccgatgagc
    24541 gaatgggaaa atctggaagc taaactgaac gaaatgccgt tggctaaaaa agacgcacgt
    24601 acttttgtgc gattcttcta ttcggctgcc actgaatgcg aacttgataa acaaggacga
    24661 atcaatatac ccagcacgtt aagaaattat gcagctttga caaaaggttg cgtagtcatc
    24721 ggtgtatcta accgaattga aatttgggat gagacacgat ggcaagattt ttctgcagca
    24781 gcagaagaga actttgacga aattgctgaa agtatgattg atttcggact ataagaatag
    24841 gaagaggctg actaatgact gaaacatttc aacactatac agtcatgttg aaagaaacgg
    24901 tagatggatt gaacatcaaa ccggatggcg tttatgtaga ctgtacactc ggcggtgccg
    24961 gacatagtga atatttatta agccaactaa gttctaaagg gcatctgtat gcgtttgacc
    25021 aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc ttcttatgtc gaacaaggta
    25081 tggtcacatt tatcaaagcg aacttcagag aattagaaga acgcttgagt gagcatgtag
    25141 ataaggtaga cggcattttg tatgatctag gggtttcctc tcctcagcta gatgaagcag
    25201 aacgtggatt tagttatcat caagatgcac cgttggatat gcgcatggat cagtcagctc
    25261 ctttatccgc ttatcacgta attaatgaat attcctacca tgaattagta aagattttct
    25321 tccgctatgg cgaggagaaa ttttccaaac aaattgctcg tgaaatcgaa cgagtacgaa
    25381 aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat cattaaacaa gtcatcccag
    25441 ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat ttttcaagct gtgcggattg
    25501 cagtaaatga cgaactcggt gccgaagagg cctctttaga acaggcaatc cggttattga
    25561 aagtcaatgg aagaatcagc gtaatcacat tccattcatt ggaagacaga attgtcaaaa
    25621 gtatgtttaa agaatatagt acggtacaag atctaccacc gggacttcct gtcatacctg
    25681 acgaattcca accagaactg aaagtcatta ctcgtaaacc aatccttcct tcagaggaag
    25741 agctaacaga aaacaatcga tcacgcagtg caaagttacg tattgcagaa aaaataagaa
    25801 tgaaagagga gtagaatgat ggcagaatta aagaaaatgc aagattacga atatgattta
    25861 cctgaattgg tggatcagcc ggaacaacaa atgcctgatt cttcttcgaa tcaagaaatc
    25921 attcttccac aatcaccggc aaaacgatta aagcagattt ctgctttgga aaaaattgct
    25981 gtggcaagcg tggcgattgc tattgttgct ttatgtgttt taacagttat gctgcgcaca
    26041 aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa cagaaatcaa tgataaaaag
    26101 caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt ctcgtactga acggattaaa
    26161 caaatcgcag aagaaaaagg attgtctatt aatgacgaca atttaaggaa agtgaagtaa
    26221 atgagtctta aaaataaaat tcggcggttt atggaaaaga aaaatctaaa tccgatgaac
    26281 aatcgcaaaa aagtaggtat cattttattt gctacgagta ttggattgtt ctttttattt
    26341 gccgtccgtt tctcgtatat cgttattgga ggtcatgttg ctggtacctc attagctgaa
    26401 aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag caaaaagagg aacgatttat
    26461 gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt attcaattaa agcgatactc
    26521 tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg aagaaaagaa ttttgataaa
    26581 atagcagaaa tcctgcataa gaatctttct atagacaaaa aagatgcact gaatatttta
    26641 gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg gtagctatgg gaaaaatatc
    26701 tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa aagaaggtgt cgctggtctt
    26761 tattttgtcg accatcaggc tcggatgtat ccaaatggcg tcttttcttc tcattttatt
    26821 ggatacgcag ttccagacaa agatgagaat ggcttagttg gtaaactagg tttggagtct
    26881 gcttacaatg atatcttgag cggtaaagat ggtaagatta tttatcaaaa agataatttt
    26941 caaaatcctc tgccaggtac agtggcagaa gaagaaaaag ctgtcgacgg acaagatatc
    27001 tacacgactt tagacagcag actacaaagt tatttagaaa cactgatgga tcaagtaaat
    27061 gaagaatatc agccagaaga attgacagca gtcctgatga aagcaaaaac cggagaaatc
    27121 ttggcaatgg gacaacgacc tactttcaat cctgaaacaa tggaaggatt gactggaaaa
    27181 gatgctatct ggcgtaattt cttggtacaa gatagttatg aaccaggatc aaccatgaaa
    27241 gttttcacta ctgcagctgc tatcgaagaa ggagaattca acgagaatga aaccttccaa
    27301 tctggaaaaa tccaagtaga agatgctacg atcaatgacc atgactttgg tgaaaaaggt
    27361 gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg tcgggatggt catactggaa
    27421 cagcgattag gcggacgatg gtataactat ttacaaaaac tgggatttgg acaaagtact
    27481 cattctggat tagatgatga agtaaacggt gctttaccta cattaaatat tgtcgaccga
    27541 gcaatgagtg cttatggaca agcggtaggt gtgacgaatt tccaaatgat gaaagctttc
    27601 acttctattg cgaataacgg tacaatgatt caacctcgat atatcagcaa agtggtggat
    27661 ccccaaactg gagaagagcg aacaactcaa acagaagttc tagggcaacc tttttcaaaa
    27721 gaaacaacag aaaaagtccg tgaatatatg agagatgttg tcgaaagcga aaactacggt
    27781 agtgcttatg gtgtatatag tgtacctgga tacaatgtat cggcaaaaaa cggaacagcc
    27841 caaatcgctt ctgatacagg gggatatcaa acaggagata cagcctacct gtattcgatt
    27901 gtggagatgg ttccttctga agatccagac tatgtcttgt atctaacaat gaaacaccct
    27961 aagacatatg atcgaatggc attggctaaa atcgcaaatc cattgatgaa acgggcaatg
    28021 gattttaaag aaactgaaga agattcagat acagaaacaa aaacagaaaa agtatccgta
    28081 gccgattatc gtaatcttga agcggacgtt gcagcagctg atgctcaaaa aagtggactg
    28141 caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac aatctactgc caacggtgat
    28201 cagttgatct ctggagaaaa actgatccta tatactggtg gtgataagct gatgcctgac
    28261 gtaacaggat ggtcaaaagc agatatcatg aagctgggga aaatcctcgg tattgaagtt
    28321 agttttgatg gagatggtta ttgtgtcaaa caagaacttg ctccatatga aaaaatcact
    28381 gaagacaaac ttagttttac tttagaagaa taagaagaga aataggagag aaaagagcat
    28441 ggaatggaca caagcattga ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat
    28501 gccgctattt attggttatt ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg
    28561 accaaagtgg cacaatagca aagccggaac tcctacaatg ggcggcttag tgtttctgat
    28621 cggctctatt ttgacaggaa tttgggttgg agcttggcaa aaacagctaa ctcctactct
    28681 tttcatcttg ctttttgtat tagcgttata cggagtaatc ggctttcttg atgattttat
    28741 aaaaatattc aagaaaagaa acatgggact gaactcgaag cagaaattga ttggacagat
    28801 tctaggggga atcatctttt atcttgttta ccgttctgaa ggttatccag gtgtattgaa
    28861 tttcttcgga ctagatctac ctttaggaat catttatggt attttcgctc tcttctggct
    28921 tgttggattt tcaaatgcag taaatctgac agatggcatc gatggattag ttgctggctt
    28981 aggaagtatc tcttttgcag cttacggtct aattgcttgg catcagcatc agtatgatgt
    29041 attaatcatt tgcttaagtg ttttaggagg tttactgggt ttcttcgtct acaacagaaa
    29101 acctgccaag atttttatgg gagatgtcgg ttcattagcg ttgggcggac ttttagcggc
    29161 aatttctata atgctaaacc aagaatggac attgcttttg attggtttga tctatgttat
    29221 ggaaacagca agtgtgatgc ttcaagttac ttcctttaag cttacaggaa aacgaatttt
    29281 taaaatgtct ccaattcatc atcactttga aatgtgcgga tggtcggagt ggaagattga
    29341 tacagttttt tggttgatca gcattgtgac ttcgttgatc acattatgga tcgtttggta
    29401 ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa taagaaagtg cttgtcttag
    29461 gactcgcaaa aagcggagta agtgcggcca aattattaca tgaattgggc gcacttgtca
    29521 ccgttaatga tggaaaacct tttgacgaaa atccggaagc acaagagctg ctttctttag
    29581 ggataaaagt aataacaggt agtcatccaa tcgaattgtt ggatgaagaa ttttcattga
    29641 tggtcaaaaa tccagggatt ccctacagtc atcctttagt agcaaaagcc caagaaatgg
    29701 gtattccggt aattacggaa gttgagctag cttacgaagt agcggaatgc ccaattattg
    29761 ggattacagg aacgaacgga aaaacaacga caacaacgat gacaggacta ctactgaatg
    29821 caggagcaga tcaaggaatt gcacgtttag cgggtaatat tgggtatcca gcaagtggtg
    29881 tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga attgtctagc ttccaactca
    29941 tgggaatcac agatttccgc cctcatatag cggtgattac caatatctat gaagcacata
    30001 ttgattatca tggtacaaga aaagaatatg tcaaagctaa atggaacctt caaaaaaaca
    30061 tgacagaaaa agattatctg atccttaact ggaatcagtc agaactacaa gaattagcgc
    30121 aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt
    30181 atgctgacga ttactcaata tactataaaa aagaaaaaat aatggaaata tctgaacttg
    30241 gcgtaccagg taagcataat gtagaaaatg cactagctgc tatttctgta gcaaaattgt
    30301 atggtatatc aaatgaagct atacgagaaa cgttgcattt tttccatggc gtaccacatc
    30361 gtacgcaata tgtaggagaa atccaaggca gaaaatttta taatgattca aaagcaacca
    30421 atattttagc gactaaaatg gctctaagtg gatttgaaac gtctaaggtc gtgttattgg
    30481 ctggcggatt agacagagga aatacatttg atgaattaat tccttcatta aaaggaatca
    30541 aagcaatggt cgtatttggc caaacaaaag aaaagttgat ggatgctgga aaaaaagcag
    30601 gcatcgagac aatagtaaca gccgattctg tagaacaagc agttccttta gcattagaaa
    30661 acagtacaga tggtgatgta gttttattat caccagcgaa tgccagctgg gatcaatacc
    30721 caaattttga aactagagga aacagattca tggaagctgt gaaccgatta aaatagaaaa
    30781 agtagaagcg agcgataaaa aatgaaaatt ttggttacag gcggaggaac agggggacat
    30841 atttatccag ctcttgcctt tgtcaattat gtaaagactc aagaaccgaa tgctgaattc
    30901 atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg tccctgatac aggaatgcct
    30961 ttccatacct tggagattca aggatttaaa cgaaaaattt ccatgcataa catcaagacg
    31021 gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca
    31081 gatatcgtta tcggtactgg aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa
    31141 atgggtattc caacagttat ccatgaacaa aacagtgtac caggtgttac caataaattt
    31201 ttaagccgat atgtggatcg gatcgctctt tcatttaaag atgcggcttc cttttttcct
    31261 gaaaacaaag cagttcttgt aggtaatcct cgagcacaag aagtagcaga tactaaaaaa
    31321 tcagatattt tgaaaacatt tgatttggat ccagaaaaga aaacagtctt gatttttggc
    31381 ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag tttttttaag tgaatatatt
    31441 ccttcttctt accaagtttt gtatgcttca ggagatagat attatgaaga gatcctaaat
    31501 aaaataaatc ctgaaataca aaatattagt gtccagcctt atatcaaaaa tatggcagag
    31561 gtcatggctt ccagtgatct gctggtgggg cgtgctggtg caacttcgat cgcagaattc
    31621 acagctctgg ggttaccggc agtgttggtt cccagcccat atgttacgaa tgatcatcag
    31681 actaaaaatg caatgagtct tgttcacgca ggagcagcaa aaatgattgc agataatgaa
    31741 ctaacaggtg aatcactaag tcagacagtc aacgaaatca tgggtgatga agaattgcaa
    31801 aagcaaatgt gccgagcttc taaagaacaa ggaatccctg atgcttcaaa acgtctttat
    31861 gacttagtaa aacaaataat caaaaaataa aggcggtggt cttagtgagt aagaaaaaag
    31921 aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg
    31981 ctaaccgaaa atatctggca gaacaagaga tgaaaaacca agagccaaaa aaagaagaca
    32041 taacattaga tacacccctt tcagattctg aagaagaaaa aacggttcaa gaggaacagc
    32101 aagaagaaag ccagtccgag aaaaaaatcg aattttttga agagatcaag caaaagaaaa
    32161 acggtggacc atataacggt tcttttttga accggctgcc caatttaaaa agtcaacggc
    32221 acaaagtact atttcgtcgg ttagctttga tcatcactgt attgggtata ccgctgattt
    32281 ttttgatcta ttatgtttct ccttatagca aattacaagc agttgcagta tcaggcaata
    32341 agacagtcaa cagccaagaa atcatctctg atacaaaatt gtcattagga gaaaatgttt
    32401 ggggacagta ttttcatcga tctacttata tcgatcgcct taaaaaagca cagccaagaa
    32461 tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt cgatttggat gtaacagaat
    32521 ataaagagat tgctttgatc gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa
    32581 cagtattaga tgaaaaagta gcaaatccta caaaaaatct ccctatcttg gaagagttca
    32641 aagactctgc aaaaatcaaa gaattaacaa agcaatacaa tcagttatca tcagaattac
    32701 aaaaagcaat ttctgaaatc aagtacacgc caagagcaag caataaaaat ctgatccaat
    32761 tgaatatgaa tgacggcaat caagttattg taaatatcaa taatcttgcg aaccaaatga
    32821 aatattattc gcaagtagcc aaagatatgg atgaaaaagg tgtgatcgac atggaagttg
    32881 gtatcttctc gcatccttac ggtagcactc aagaaacgga agaatcgggc caatcggaag
    32941 aaacaactga aagtgaagaa aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa
    33001 cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt
    33061 caagtgaaac aagtcaaata gaagataatc caagtacaac tccgtcaagt ttaaatgaaa
    33121 attgataaaa caactttaaa aagtaagaat gtactttcta aaaaccagta atttatggta
    33181 gaattggaag cagtgaatat atctattaat aagtatattt taaaacggac gctagttgtt
    33241 cgcttgtata tggaatagta agtaggaggg aatcccattc atggcaaaaa caggaatgta
    33301 tgtaggcctt gatattggaa cgacatctgt taaagttgtc gtggctgaat atatcgacag
    33361 ccaaatgaat attattggag taggaaacgc aaaatcagaa gggattaacc gaggcattat
    33421 cgttgatatc gataaaacgg tccaagcgat acaacgagca gtacgacaag cagaagaaaa
    33481 agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga
    33541 aaattgccag ggaatgattg cagttaacgg agattcaaag gaaatcaccg atgaagatgt
    33601 cagaaatgtg gcttcagctg cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc
    33661 aatattgcca caagatttta ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat
    33721 gatcggcgta agattagaaa tgtacggtct gttgtttaca ggacctaaaa cgatcatcca
    33781 taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc aacgaaatgg tgattactcc
    33841 tttagcgctg actgaatcaa ttctttctga cggagaaaaa gattttggta cgatcgtcat
    33901 tgatatgggt ggcggccaaa caacaacggc tgttatgcat gataagcaat tgaagtttac
    33961 caatcttgac caagaaggtg gcgaatttgt cactaaagat atctctattg tattaaatac
    34021 gtcatttaac aatgcagaag cgttgaaaat aaactacggg gatgcatacc cagaacggac
    34081 atcaccagat gaagaatttc cagtagatgt gattggtcag tcagaaccag tgaaagtcga
    34141 tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa cagattttca acaaagcaaa
    34201 agatgcattg gaccaaatcg aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg
    34261 agctgctagt cttcctggtg ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa
    34321 attgtatgtt ccaaatcaca tggggctgag aaacccggta tttacaaatg taataagtat
    34381 cgtggattat tcagcaaact tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg
    34441 tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa acaacaaaca catatgagag
    34501 ctatgaagca cctgaagaaa caatctacga tgagccggaa ccaaaagagt ccggagaaaa
    34561 tgtaaaaaat aaaatcaagg gtttctttac aaatattttt gattaattca tcctgggagg
    34621 aataagatat ggaattctca attgataata atatcaatga cggcgcagta atcaaagtca
    34681 tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat gattgaagaa aacgttaaag
    34741 gtgttgaatt cattacagcc aacacagacg tgcaagcatt aaaaaattca aaagctgaaa
    34801 cagttatcca attaggcccc aaatatactc gtggtttagg tgctggttct cagccagaag
    34861 ttggacaaaa agcggcagaa gaaagtgaac aatccttacg cgaagcttta gacggtgcag
    34921 atatgatttt catcactgca ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg
    34981 ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca
    35041 cttttgaagg accgaaacgt ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa
    35101 atgtggatac attattaatt atctcaaata accgtttatt agaagttgta gataaaaaga
    35161 caccgatgct tgaagctttc cgtgaagcag ataatgtttt acgacaaggt gtacaaggta
    35221 tctctgattt gatcacagcg ccaggttacg ttaaccttga ctttgctgac gtgaaaacag
    35281 tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg
    35341 tagtagaagc aacgaaaaaa gcaatctcct ctccattgct tgaaacatct attgatggag
    35401 ctgaacaagt acttcttaac atcacaggcg gtttggatat gacattgttc gaagcacaag
    35461 atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat
    35521 ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg
    35581 aatcaaaaaa ggaacgcaag tctagccgac cagctagaca agcacaaatg caatcacctg
    35641 ctcaaaagac tgtattagat atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca
    35701 gttttggcga ttgggatatc agacgtgaac aaaatgttcg tcctagagtt gatgattcca
    35761 attttgacaa tatcgaaaaa aaagaatttg atacattcaa ccgcgaagaa acaaaatctc
    35821 atggcgatga tgaattaaat acgccaccgt ttttccgtcg taaaaaataa aggagaggaa
    35881 gagcacatga ttgctgacaa cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct
    35941 cttgttcatc gtgatccgaa agaagttaca ttagtagctg taacaaaatc tgttgaatcc
    36001 gctcaagctg aagaattggt tagagctggc gttatgaatt gtgcagaaaa ccgagtagat
    36061 aaattattag aaaagaaaca agatttattt gattttcctg aaataaagtg gcatttaatt
    36121 ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg aaattgatta ctttcatgcg
    36181 ttggacagtt taagattagc agaagaaatc cagaagcggt tagagaaaga attatcctgt
    36241 tttattgaag taaatgtttc tggtgaggaa tcaaaacatg gaatttcacc agatgagctt
    36301 ttaccgtttg tggaatcttt agctgcctgc acaaaaatca aggttgttgg gttgatgact
    36361 atggcgccta aagaagcttc tgatgagcaa atccataaag tgtttggtac gctgaaagaa
    36421 ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac catgtagtga attaagtatg
    36481 gggatgagtc aagactttca aattgcgatt gaagaaggtg caacatttat ccgtgtagga
    36541 acagcgctat tcaaagacga ataaaggagg aacaaaaatg ccgcttttaa acagaggagc
    36601 aattgctaat tttttcggat tagctgatga ggaagagtac gaataccaag attatcctga
    36661 aacaacaaaa aaggctgtac agccaactaa aaaaaataca acacagaatc aatcaactca
    36721 ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac
    36781 gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt gaactacatc aatcacctac
    36841 aaaaactgta gcgaaagaaa gaacagcttc aagaacctct acacaaccta aagcaaatct
    36901 tgctggaaaa atcactgtaa tggaaccaag agcttattct gaagctatgg cgattgctaa
    36961 acgaatcatt gctggagaag caaccttagt gaattttcat ttagtagaag aaaaacaagc
    37021 gagaagaatc gttgattttc taacaggaac agtttatgca ctagatggag atatcaaacg
    37081 tgttggagat gagatttttc tttgtacacc gactgggatg gagattgaca gttcaaccgc
    37141 tcaatctttt gcagatgcga atttgtttga tctgtaagag gaggaactac gatttatact
    37201 attgcactat tacttcattt acttcaacgg gcagcctatt tctatagcat tatcttgatg
    37261 gtatatgcat tgctctcttg gtttccaggc gggtatcaat cagggctagg acgattatta
    37321 gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc tgcctttgag gattggaccc
    37381 ttggatttca caattttagg ggcaatcctg atcttgaact tagccgtaca aggtttagca
    37441 cagatcgtta tatggataac tgtccattta gtttattgat tgaagcaaca aaatctttat
    37501 tctcagaata gataggaggg gaaaattttg gatgcaaatg tataccagca ctttcgggca
    37561 gatgaacgta catttatcga ttcggtaggc gattggattg aacaggttca aggacaatat
    37621 gcaccctatc tgaccgaatt tcttgaccca agacaatcct atattttaga aacactgatt
    37681 cgacaaagca gtgatttgcg tttcatgttt tatggcggat atgaacaggc agaaagaaaa
    37741 agatgtttga ttttccccga ttattacgaa cctgtagagg aagattttga aacagcattg
    37801 tatgaaatcc attatcctag caaatttgcc agcctaagtc atgggaaaat ccttggaact
    37861 ttagtgggaa cggggatcaa acgctcttac tttggcgata tcatctcgga cggtgatcgc
    37921 tggcaagtat ttattgcaaa tgaggtggat cattttgttg ctgcacaagt aacaaagatt
    37981 ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag aaatgattat gccgaaagat
    38041 agctggacac aagaaagagg aacagttagt tccatgcgac tagacagtgt gatctctgag
    38101 atttataata tttccagaca gcggtccaag cagctgatcg aagctggtaa agtaaaagtg
    38161 aactggacag aaaatacaag acctgatttt gtacttgaac tattagatat tatttcagtc
    38221 agaggttatg gcagagtcca gatccaagaa cttgagggga agacgaaaaa agaaaagtac
    38281 agggtactgt tcggtgtatt acgtaaataa aataaaaagg tggtttactc atggctttaa
    38341 ctccattaga tatccaaaat aagacattcc cgacaaaaat gcggggatat aatcaagacg
    38401 aagtagatga tttcttagat ttagttgttc gtgactatga ggaattgact caaagaaatc
    38461 gagaattaga aaaagcagtc aaacattctg aagaaaaact tgagtacttc aatgaattaa
    38521 aagatgcttt gaaccaatca atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa
    38581 gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca aaacaaagca gatgagttag
    38641 ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga tgcagaagaa aaagcaagaa
    38701 aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc gacagaaact gaagatctta
    38761 agaaaaaaac acgagtattc catcaacgta tcagcttgat gcttgagtct caattagaac
    38821 aagtgaaaag tccggaatgg gatgaaattt tacaaccatt ctccagctac gtgactgaca
    38881 gccatgaagt gatcaaagaa gttttagcaa aacagcttga caatgaaaat gatactgatg
    38941 taaactcaga cacaggagtc atagaagcac ctatgacggc atttgataca caggctattg
    39001 atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt
    39061 cttaaagcga gccgatgaga gtggaagttc ggtaagacac tgcatggtga aagatcctct
    39121 acttgagacc tgcttttccg aaattagagt aagaagcagc ggctgacctc gttattggtc
    39181 acagagagtt tgcactagat agatgcaact aaatttgggt ggtaccacgg catgttcgtc
    39241 ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa
    39301 tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc
    39361 caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa tcgaatctac caaaaaagac
    39421 aagagttgaa cgaaggaaaa cctagctttg ttcttcatga tggacctcca tatgccaatg
    39481 gtaatatcca tttaggacat tcattgaata aaatcagtaa agacattatc attcgttcaa
    39541 aatcaatgtc tggctatcgt gcaccatatg ttccaggatg ggatacacat ggattgccga
    39601 ttgaacaagt attggctaat aaaggcgtga aacgtaaaga aatgtccatg gcagaatact
    39661 tggaaaaatg tcgtgaatat gcactttccc aagtagataa gcaacgagca gatttcaaac
    39721 gcctaggagt tgcaggagat tgggaacatc cttatgttac attagatcct tcttatgaag
    39781 ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaagg ctatatttac aaaggattga
    39841 aaccaattta ctggtctccg tcaagtgagt cttctttagc ggaagctgaa attgaatata
    39901 aagatgtaaa atctccatct atttttgtag ctttcaatgt caaagacggc aaagatattc
    39961 ttgatgaaga tacagctttt gtcatttgga cgacaacacc atggacattg ccagcaaacc
    40021 aaggtattgc tgtaaatcca aaatttacgt atgtattggt ggaagccgat ggccgtaagt
    40081 ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa
    40141 cagttctaaa agaatttgct ggacaagaaa tggaatatat gactgcaagt catccattct
    40201 atgaacgtga atcacttgtg attttaggcg atcatgttac tttagacgct ggtactggat
    40261 tagtccatac tgctccagga catggggaag atgactatat cgctggtagc aaatataaac
    40321 tatctgtagt gtcacctgta gactcaaaag gattgtttac agaagaagca cctggatttg
    40381 aaggtatctt ttatgacaaa gcaaatccta tgatcactga tctattaaaa gaaaaaggag
    40441 ctttgctgaa gctagatttc tttactcata gttatcctca cgattggcgg acgaaaaagc
    40501 ctgtgatcta tcgggcaact ccacaatggt ttgcatctat cgataaattc cgtcagaata
    40561 ttttggatga agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca cgtttgtata
    40621 atatgatccg tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac
    40681 ttccgatttt ctatgctgaa aatggagaag caatcatcac accagaaaca atcgaacatg
    40741 tggcaaaatt atttgctgaa catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac
    40801 ttcttcctga aggatttacc cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag
    40861 atatcatgga tgtctggttt gattctggtt cttctcatga agctgtttta cgtcagcgac
    40921 cagaattatc attcccagca gatatgtatt tagaaggatc agaccaatat cgtggctggt
    40981 tcaactcaag tatcacgact agtgtggcta tcaatggtgt agcaccatac aaatcagtta
    41041 tttctcaagg atttgcttta gatggtgaag gccgtaagat gagtaaatct ttaggaaaca
    41101 tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc ttatgggtat
    41161 ctagtgtaga ttatgaagca gatgttcgtg tttctatgga tatcttaagt caagtggcag
    41221 aagtatatcg taaaatcaga aatacgatgc gtttcttatt agcgaataca gaagatttcg
    41281 atcctaaaaa agatactgtt tcgtacaatg atttacgttc tgtagacaaa tatatgaccg
    41341 ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa tataacttca
    41401 tgcatattta ccgtacagtt atgaacttct tgacggttga tctatcgtca ttctacctgg
    41461 attttgctaa agatgtagtt tatattgaag cagaagatga ttatcaacgt cgttgcatgc
    41521 aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt attgactcct atcattccgc
    41581 atacagcaga agaaatctgg tcattcctga aagaagaaga ggagtacgtt cagttaagcg
    41641 agtttcctgg ttatgaagag tttgcaaacc aagaagaatt gatggatacc tggactgctt
    41701 tcatggactt ccgtgataat gtcttaaaag cattggaaga agcaagaaac agcaaattga
    41761 ttggtaaatc tttggaagca aaactaactg tttatccaaa acaacaagtt cgtgaaatgt
    41821 taaaagcttt ggatgcagat attgcacaat tattgatcgt ttcaccagac tactttgaag
    41881 ttatggcagc agatgaacaa gtgccagata acgctatggt atttgatgat gttgctatta
    41941 cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag
    42001 gagtagatga aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct
    42061 atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat
    42121 gttctttgtc acaccccctt ttgttatcaa tcgtttaatt ttccacgttc acgataccag
    42181 ttatctggtt gccagatcca ttcgaatccg tctttttcaa gtagttcgaa agctgcttta
    42241 ggtcccattg atccagcttt gtagttggga aaatccggtt gtgtttggtc ccaagctttt
    42301 cgaatgacat ccacgatatg ccaagattga gcgacttctt cccaatgaga gaagttcgtt
    42361 ccatcaccat tcaacgcatc taatagtaat ttttcgtaag cttctgggct attttcaacc
    42421 atttctgcac tatttctaaa ttccaatttg attggttccg tttcaaatcc ttgccctact
    42481 tctttcccat tcaaggataa agaaaatcct tcagttggct gaatatagat cgttagaaca
    42541 ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag atgttttgaa tacattaaca
    42601 ggaacttgtt tgaatacgat gttgatccga gtcccttttt cagtcaaacg tttgcctgta
    42661 cgtacataga aaggaacacc tgaccaacgg aagttatcga tagtgaattt gcctgcgacg
    42721 aatgtttcag tctctgattg agcatccact ttgtcttctt cgcgatagcc cttgaattgt
    42781 acaccatcca attcaccttg agcatattgc ccgcgaacga agttttctaa cacttcttct
    42841 tcgttataaa tgcggatagc tttcaatgct ttgatctttt ctgtacggat ttctttttcg
    42901 gaaaatgcta ctggcggttc cattgcaagt aatgctacaa cctgtaaaat atgattttgc
    42961 accatgtctt taagtgcgcc actatgatcg tagtagcccc cgcgatcttc tacaccgagg
    43021 ttttcgccaa aagtaatttg gatattgtcg atatagcgat tgttccattg agcctcaaaa
    43081 atattattgg caaaacggat agctgagatg ttttggatca tttcttttcc taaataatga
    43141 tcaatcctga aaatatcttg ttctgggaat acttctctga tttgctggtt caattcaaaa
    43201 gcagactggt aatcagaacc gaacggtttt tctatgatca aacgattgaa gccatttttt
    43261 gtcataatat tttgtgattt caaatgagaa acgatcgttc caaaaaattg aggtgccata
    43321 gccaaataat agacacggtt accgtttaat tgatagtgtc catctaatcg gtcagacaat
    43381 tcttttaatg tctgataatg cgttgaatca tttacatcat gggattgata gtaaaaatgg
    43441 cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg cacggattgt ttctttgaca
    43501 acttcacggt agtgctcatc gctccaggga cggcgagcgg ttccgattac agcaaaatgc
    43561 tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg ggtacaattt acgctgggct
    43621 aggtcacctg taccgccgaa tatcgtaaac aatacatttt tttcattaga catgtggata
    43681 cactccttta tattttatcg acaaaaatcg tgctggccgc cttaaagctg acggggattt
    43741 tctttctatc attttggata gtgatcggtc cttcataagc tgcgatgtca acgatctcat
    43801 attcttcgtt gatattcagg tcaatagtga ctaagtaatc caacaattct ttttcatcaa
    43861 gcactcgtgc gatacgcact tttgtaccga caggatagga ctccaatgtt tgtcggcgtt
    43921 cttcatggac aacttcctct tttttaggaa tcgatcctcc atgaggacaa tgttcaggat
    43981 gattcaaata gttttctaaa tgatctgcaa gatgttccga agtcacatgt tccaatactt
    44041 cagcatcatc atggacttca ttccagctat agtctaaatg ttcaactaaa aaaacttccc
    44101 ataagcgatg tttgcgaatc agactgctag ctttttgcag tccgcttgca gtcaattgta
    44161 caccttgata aggggaatgt tctacaagct cttctttcac aagtttagaa atcatttcac
    44221 tgacagatgc agctgagaca tctagactag atacgatttg tttattgttg actttcgttg
    44281 tatctccacc tagttctaaa atgattttca aataatcttc tcgatttggt gtcatattcg
    44341 atcatccttt catggtaaaa aaccatcatt agtaagtgta acaaaaaaaa cgttaattga
    44401 ctatttttct aaataaaaaa caaaggattt ttagacgttt ttacaacaaa aaacgaaaga
    44461 tgatagagaa gactcattct ttcgtttata aaggatatat atatacattg ggagggatct
    44521 atttttcaga taacaactta tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg
    44581 gataagaaaa gataagataa aaaccatact gatcacgtac gactaaacga tattcatcta
    44641 tttctaccac agatgtgcca agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa
    44701 tctgtaattg ttgtttttgc tggctatcac taaagaacca tgtaccttga tactttgaga
    44761 gtccttctga cttttccgct tgttttttct ttttcaagta aataaaactt gagagaacgg
    44821 taatgagtga accaagaaaa tagatcattt tttttatcac caaatcactc ctttatgaga
    44881 aaataatcgc atagacatat tctttcttct ttatttcatt gtaccgaata aaagctaaaa
    44941 tttctctaag actttcgact ggttttttta ctgtttttct acaaattatg ataggataca
    45001 tagtaggaag aaaggtgaca taatttgaag tatcagcaag tggaaatagc tttttttatt
    45061 gaccgcccga atcggtttat agcacattgt atgaaaaaag acggagaaat aattattgcg
    45121 catgtcaaaa atacaggtag aggaaaagag gtcctgatac caggcgctga agtggctatt
    45181 gtatttgctc ctggacctaa acggaaaacc gcctatgatc tgattgctgt aaaaaagaag
    45241 ggtgactggt ttaatatcga tagtcaattg cctaatcggc tggcaattga tggtatttta
    45301 gacggcacga tccttttacc aggattaact ggtccattta cggtatacaa gcgtgaagta
    45361 acttatcttc actccaaatt tgatatttat ttagagacgg tggtcggaca aaaagcattt
    45421 gttgaagtca aaggtatgac acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc
    45481 acactcagag gattgaaaca tataggagaa ttaacgtatg ccgcacaaga tggttatgcc
    45541 gcttatgtct tgtttattgc tcagttcgaa catcttcatc tggcaacgat ccatgaagaa
    45601 atgcaaccag cactagctga tatggttcga catgcgcagc aaagcggagt tcaaatcctc
    45661 gcctataatt gtcaagtatc ggcagatgag gtaactgtca agcaggccat tccatttgat
    45721 gtagatatcc cttttgaaga tcctatacaa tagaaagaag gtaaaataat gatcaactta
    45781 ggtattatcg gaacaaattg gattactcat caatttgtcc aagcagcttt aagcacaaaa
    45841 aaatatgaat tgacagcggt atattctaga catttggaaa aagcacagga atttggttct
    45901 cattatgaag gggatatcga attcgcaaca gatttgaaga cttttttcaa tattactcat
    45961 atggacacca tttatattgc ttctccgaat agtcttcact ttagtcaagc taagcaagca
    46021 attttggcag gaaaaaatgt gatagttgaa aagccagctt tttcgacgcc aaaagaaatg
    46081 gatgaaatca tccatttggc aaatgaacac aatgtgtatt tttttgaagc agcaagaaat
    46141 atccatgaat caggttttaa aaagattgcc gattacttac cattaaaaga cgagataatc
    46201 ggtgcaaatt tcagctatat gaaatattct tcgcgctatg accaagtatt agatggagaa
    46261 gaaccgaata tcttctcgac tcatttctca ggtggtgcat tgatggattt aggtgtttac
    46321 cttgtatatg cagctgttgc ttggtttgga atgccaaaag aagtccatta tttcccaaga
    46381 aagatttcta ctggtgtaga tggtcttgga tgggggatct tacgttatga gaattttgat
    46441 gtatcgatcc agccaggtaa aatcggtgat tcttatttac cgtcagagat ttattttgac
    46501 tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac aagcacaatt tcatgatcgc
    46561 gcgcataaag aaatcattga tttggcggta acagctgcag aaaatccaat ggcagatgaa
    46621 gcagaggatt ttgccaatgt catggctcat ccgaaagatc cagcttgggg attgttgtat
    46681 gaagagtgga tcgagctagc tagaaatgtg aaccaagtga tttatgattt aagaaaaaat
    46741 ggcggcatca aattcgatgc agataatgag gaagattttt aatgaacgaa tttgtacaag
    46801 tatttcccga aaattggcag aaaaaatatg aggataaagg gtttatctca ccttctctta
    46861 ttcaacgagc agtttttcag ccactaagta ataaacaaag tatagtagcg gtttctccta
    46921 caggttcagg taaaacactg gcttatcttt ggcctctact actaaacgtg gaaccaggtg
    46981 aagcaagtgc gttggtcatt tttgcttctt ctcaagaact agctattcaa gtagcagatg
    47041 ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca atctttggtg ggcggagcca
    47101 acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag
    47161 gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg
    47221 tatttgatga agcagaccag ctatttgacg ctgggaacag ccaaatcatt gatcaaattc
    47281 ttcatcaagc tccaacagag tatcagctag cttttttttc agcgacagca gatcgttccc
    47341 ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac aattgacgtg acagcagagg
    47401 acgattctcg aaaaggactg agacattact ttttacgtgt accgatacga aagaaagatg
    47461 actacttgag acgtctcacg catatagaag attttcatgg tctgattttc tttaatcaac
    47521 tgactgaatt aggcgtaatg gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc
    47581 tggcctctga tcagaacaag ctactccgaa aagcagcgct agaacaattc aaaaagaaaa
    47641 tggtcagagc acttttgacc acagatgtgg cggctagagg tttagatatt acaggtcttc
    47701 cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa
    47761 gaactggtcg aatgggtaat gaaggaatag taattacact cgtacaagat aataatctaa
    47821 gagatttaaa acggctttta cgtccatcaa atataacact tgaagagatt tttttatacg
    47881 gaggtaagct gcaaactgag cagccaacca aagaagatgc agctcctgca gtaaataaaa
    47941 agtatgcgaa aactttttct ggaaaggagc agaacccaga atataacaaa gaaaaaataa
    48001 gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa aggagcaagg cgcaaataag
    48061 cgccttttta tgctatgatt atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta
    48121 tgtgaaagaa cgtcaagaag agatcttgca gctattgaca gaaaaccaac aaggattgac
    48181 agcaagtgat gtagctgagc gcttaactat cgatcgaagc aatgcaagcc gatatttaag
    48241 tgaactatac aaggcccatc atattgtaaa aaccgctggc cgaccagtcg tttactcttt
    48301 gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt
    48361 tgaaacactc gttggagaaa atgattcttt aaaagtcagc atacagcagg caaaagctgc
    48421 gattctttat ccaccgcggg ggttgcatac gattattttt ggagaaactg gtacagggaa
    48481 gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat tcagagatgc tttctgcaga
    48541 tgcgccattt gtttctttca attgtgcgga ttatgctcaa aatccgcaac tgctctttgg
    48601 acacatcttt gggatcaaaa aaggggctta cacaggagca gctcaagata gtcccggtct
    48661 aatagccaaa gcagacggcg gtatattgtt tttagatgag atccaccgtt taccgccgga
    48721 agggcaggaa atgctgttta ctttcattga taaaggtatt taccgtccgc ttggagaaag
    48781 tgcacaagta catgaagcat ctgttcagat cattggggcg acgactgaat cttcagaaag
    48841 tttcttatct acgttcaatc gacggattcc tatggcaatc accttgccga atcttgcttc
    48901 acgttcactg gatgagcgtt atgaaatcat ctctcttttt atcaaacaag aagcgaatcg
    48961 tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt gccttcatgc tttatgatgc
    49021 ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt
    49081 acattaccgt actcatcatg agaaaaagct aatcattcga aaagaagaac tttcccttca
    49141 agtacaaaaa ggattactga aaatcaagga aataccggaa cgtctcgacc gattcatgga
    49201 cagcaaaagt cagtatttga catttgaacc aggatttgct gatgttgtct ggtcacaaga
    49261 tccagaacga aacatgcagg tctataacga tattgaggaa aaagtcactt ctctttccga
    49321 aaccggtgtg gagaacattg atctagagac cctcatttca aaagatgtgg atgcttattt
    49381 ccagacatat gtcaaagaat tgactaaaag ttcgattccc aaagatcttt tgccagatga
    49441 aatctggcgg ttaaccaatc aattgtatga tgtggctgaa aaacggttag accgtattta
    49501 taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa agtacattgg atcgtgtgag
    49561 agaaggtcat atgatcgtcc acccagattt gaataacgta cggaaaaatt ttaagaggga
    49621 attccaggta gcaatcgatc tttcaacgat cattgaagaa gaagaacaag ttgagattcc
    49681 ttttgatgaa atcggcttta ttagtatgtt tctttctatc aatctaggag aaagtgagcc
    49741 tgttaaagaa aacaaagtag atgttctcat attgatgcac ggcagacaga ctgcttcaag
    49801 catgttggag atggcacaag aactattagg aacaaaagta gggaaagctt ttaatatgcc
    49861 actggttatg gaagttcaaa caatgtatga agaagtgtta caatatgtca aaaactatca
    49921 agagcatttg acgaatggcc tactcttgtt aacagatatg ggatcattaa acaccttcag
    49981 tgatttgatt tatgaagaga ctggcattcg gacgaaagct atcacgatga caagtaccat
    50041 gattgtgata gaagcgatac ggatggcaag tgttggaaga agcttagaag atatttatca
    50101 aaatatccag ctttcctttg aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga
    50161 aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt actggtgaag gagtagctgc
    50221 caaattatat cagcgtatta tgccagtgat taatcaaaat aaggtagaac tgatccagat
    50281 gcagttttta gaacgtgaat cattcaaaaa acacatcgat gccttattgg aagaatatga
    50341 gatcaaagca attgcgggaa cagttgatgt tgattatcaa aatatcccat ttttttcagc
    50401 ctatgacgtt tttgatgatg aaaaactaaa tattttaaag cgtatagctg atgatgagat
    50461 cccgacagct aaaattgtcg attcacttgc tggatcatta agcgatgtct tttctattga
    50521 tgaactgatc cgttcattgc aaaagacagt tcagcagatc cagaccgatt tgtcgattat
    50581 cgtggaacct ggagtagata ctggaatcgt tatccattta gccttcatgt tagacggatt
    50641 gatcaaacaa gaaagccatc ggcaatttga gaaactgtct attttctcaa aaatttatcg
    50701 tttggaaatg gatatcgttc gggcaaactt gatggcacta gaacgaaaat atcaggtaaa
    50761 aattccagaa gatgatatcg catttcttac acaaatgttt cttgaaaata aaataaaaaa
    50821 atgaacacac taatcatact gtttacacac taaacatctg tgtgcgcact ataaaaaaga
    50881 gctaaaaagc atgaaaacgc tatttcagct ctttttttta ttggcatgct tcttgcgtta
    50941 tataaaagtg taaaaaaata aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag
    51001 tttgttcagc agggatgagt acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag
    51061 aaaaagggat ggaagccgat atttttgcgg tctctgcctc agatgcagat aataatttag
    51121 aatctaagaa tgtcgatgta ttattattag gtcctcaagt acgtttcatg aaagctcaat
    51181 ttgaacaaaa acttgcgcct aaaggtattc cattagatgt catcaatatg caagactatg
    51241 gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt
    51301 atgcagttgg gggtataaca acgtggaaga tcaacaaaat ctagaagcca tcatggggtt
    51361 aatcatgttt ggcggtaatg caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat
    51421 cggtgatttt gagttagcgg ataggaaaat cgctgatgca gaagaatcac ttatacaagc
    51481 gcatcatgca caaacagaca tgttgacaca agaagcacaa ggaaatcata tgcaagtgac
    51541 attattgact gttcacagtc aagatcactt aatgacatct attgcgttta ctgatttagc
    51601 aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa taaatacaac ttcttttttg
    51661 aacggaagaa gaaaaatttt tgaacggatt ttttattttg ttataaggag aactctcttt
    51721 atataaaaaa cttataggag gaagtaaaaa atgaacggac tatcaagttg gatggagaaa
    51781 tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat
    51841 gcttttatcg gcatactgcc cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca
    51901 attattcgtg atttaccgca acagtttaac agtacctatg ctgacagttt agcagcagac
    51961 aaaggaatct ttgccgtatt gaatgtcatc atcggaatca atggttttgt ctggaacggt
    52021 acactagcaa tcgccggatt gatctttgcc ttttcttggg ggtataatct tgcccgagct
    52081 tatggagtaa atgatctagc tggggggatc gttggtttag ctaccttgat tcaaggtatt
    52141 tcctttggtc cagcattatc agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat
    52201 gcaatcaata gttctgagat tggtgcaacg attgcagatg gcacactgtc agttggatta
    52261 tggggttggc taaaactaga tcatttgaat ggaaacgctt attttacagt tatgatcatg
    52321 ggagctattt cagtcattat tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg
    52381 cctgattcag taccaccagc agtatctaaa gcatttgcag ccattttacc agcgacgatc
    52441 gctttatacg tcattgctat tatcaattat atcgtaggtc gtgtagctga tgggcaactg
    52501 atcattgatt tggtgcaaaa atatattgca gaaccattcc tagggttatc tcaagggttg
    52561 ggagcagtat tgatcgtcac agtatttgtt caagtattct ggttctttgg tattcatgga
    52621 tcaaatgtat tggcaccagt gctagaggga atctggggac aagcacaact aatcaatatt
    52681 gatattttcc aaaaaggtta taacggaaaa actggtacac cagccgtgtt agatgcaatc
    52741 gaagatggaa aagcttatat gtgggtccgc ggatcgttcg atgcttttgc atggtttggc
    52801 ggatcaggcg gaacgatcgt cttactgata gctatcttga ttttctctaa acgtgcagat
    52861 tatctaacag tagctaaatt atccattggg cctggtatct tcaatatcaa cgaaccagtg
    52921 atgtttggtc tgccaatcgt attgaatgca atcatgttta tcccattctt gattgctcca
    52981 gtagtagcaa cagcaattgg atggacagct acttatcttg gcctagtcgc tccggtatcg
    53041 cagcaagtca catgggtagt accgccgttc ttattgtcat tcttagcaac aggagcggat
    53101 tggcgggcac cgatcgtaac attagtatgt atggtcgtga ctttcttgat ttgggcacca
    53161 tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg aacaacaata agatgaacgt
    53221 gaggagcggg acgctttaag tttcgttcct cataatgaat tttcgaggag gaactgaaat
    53281 gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa
    53341 tgaagaagga atgactttta catttaaaga aaaacaagga atcaagctgt tctttgaaac
    53401 aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag gaactaatca aagcacagcc
    53461 ttggggaaca gtcctttatt ttcaatcaac agctgtttag aaaatggttt aaaaagatag
    53521 gatggttgag atgataaaaa acggtttatt tttatgtagt attggttctt tgatcgtgtt
    53581 gtatagtttg aatccacatt cgcaaaggta tgatttgtat agtatggtca caggtctttt
    53641 tctaacagga ttaggaatct atttcttcat gaaggggaaa aaaagagaag aagcaaagga
    53701 gaaaaataac taatggaaat cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat
    53761 aaagttatgg actctgtcct tttcgatgta cgtaaagcga ccggcaaatc agtaactaga
    53821 aaacagctga aaaatttgga gtacgtcaaa caattctcgc aaaataaccg ggctcgcata
    53881 aaaatcgaag aagtcattga aaaccagtct tataagtttc gtacatcgac aacgaagaat
    53941 gaattcgtcg ttcattatca aatcaaacca ttggacgatt cgacctgtga ggttcattac
    54001 acagaaaaaa tggagtctta tggcatgctt caaaagttga atgatatgct tttaggaaca
    54061 atcttgatgt attttaaacg gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg
    54121 tattaaatca atgaaacatg gtactattta gttattgaaa aatgaagtaa gctagaagga
    54181 atttttcata ggaagataga tctttcctgc gaatcctcac tagctttttt gtaggaaggg
    54241 gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc ttcgattgag gaaatgtttg
    54301 atttagccac ttatgcattt aatggggcag atacacctga aaggcgagaa cgttttcgta
    54361 cacttgcgga gaattcttgg aattacggat tttttgatga ggaggatcgg ctaacaagtc
    54421 aagtcatggc gacccctttt ccagtgaatt tttacggaca agagtaccta atggccggca
    54481 ttggctatgt tgcttcttat ccagaagcaa ggggacaagg cgggatcaat cagatcatgg
    54541 aaaagatcct tgaagattgc cgtgatcgta aagtaagctt atcttatctg gcgccattct
    54601 cctatccctt ctatcggcgc tatggctatg aacaatcatt tgataaaatc agttatcaac
    54661 taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt
    54721 ttgaagatgc aaaagctgca atcagaagaa tttatgaaca gatgccggaa aaccaacgag
    54781 ctggtctgaa gcgcgaagac tggtggtata cgtataaatt caagcaaaaa ggcaattacc
    54841 agtttgcttt gcattttgat gataatgagg aagcaacggg atatatcgtt tatcagcttg
    54901 caacgtcttc ctttattatt gcggagtggg gatttttgga tcatgatgct ttttgcagcc
    54961 ttgttcgctt cgtaagttct cacaatggtg cttttgaaac atttgaatat tcatgcggct
    55021 acggcggtaa tgatcaaaat tatctattag aaaatccatt tgcttctgta atgatcacac
    55081 cttatatgat ggcgaggatc gtagatattt ctttgttttt ggaaaattat ccatttaaaa
    55141 agagacaagc tgcatttgcc ctacaaatag aagaagatca ttatgcgaaa tggaatgaag
    55201 gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa
    55261 aactcccaat cgttcgagga agtatccaac atatcacgca gctattatta gggtatcgta
    55321 aagtggaaga gttggtattc caagaaagaa tacaggtgac aaaagaatat gaagaaacgt
    55381 taagctcaat cttgccacag caacagccaa ttcttaatga ctatttttga taaaaaacgg
    55441 gtgatgacaa tatttttgtc accacccttt tgtttttcat ttggcatttg aaggaaaatg
    55501 ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg ttttcttttg ttttagggtt
    55561 ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt tcccgatttt ttttagatga
    55621 atctaagcgt tctgcgtata ctttttcttt tggcttatca ttcattggaa aggacctccc
    55681 tacattattt cattctacct ctagtttatc atctgttaag gactagtgga aacgttttgc
    55741 ctaacttata agctgatttt tttagcagat aagcttgcaa gtaaaaaaca aacaagctac
    55801 aattgattta ttgaaattgt tcctgtagct cagtaggata gagcaatcgc ctcctaagcg
    55861 ataggtcgct ggttcaattc cagtcaggaa cgtataaata acccctgtat cctttgcggt
    55921 acaggggttt gttttgtatt tgtgccaaaa atgtgccaaa ctagttatca tttttatata
    55981 aactatcgaa cacagtattg attttttctt tatcctgatc ttcaagctct cgaacaatat
    56041 gaagataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa
    56101 gtatatttac tccttgataa agtaaaatag atgcatgtgt atgccttagt ccatgcaaag
    56161 taaattgctt ttcaatccct aattctttta attttttgcg caaatatttg ctgacggcgt
    56221 ttgatgaaac taatccattt tttaagttga aaaaaacaaa attatgaggg tttttgattt
    56281 caaaattctc gaataattct ttttgattga ttttaaactt ttttaagaga tcaatcgtat
    56341 gtttatcaat ggaaattttc ctatttgaag tttcgttttt tgtttttccc cattcattta
    56401 atttataatt ccatgtttta ttaatagtga tgatttgatc ttcgaaatcg atatcttccc
    56461 atgttaatcc tagaagttca gcaaatcgca ttccagtggc accagcaact aacacgagca
    56521 taggagaaga gtactgcgca gaaaggtttt cttctacgag tttcattaaa gatttaaatt
    56581 gatcataatc taaatattta tcttcttttg gcttcaatga atcttttcct ttgattactg
    56641 cttttctagt tggatcaaac ggaattaatc cttcttctac ggcatctttc aacgacgctc
    56701 taatatggtt attaaactta ataacggtag attttacatg gtctttagca tacttattta
    56761 aaaagcgttg atatcccact ctatctaaat cagaaatcaa taccgctggc atatatttct
    56821 ttatgttcat taacgtatct tcgtattttc tataagtgat aggagatact gttccttctt
    56881 tataaagttg catccaatcc tcaaagtaat ctgataataa tagatttttg cgatcccctt
    56941 taaggccttt gttcaagtta tattctaatt cgttggcggc atcttttgct tctccttttg
    57001 tcttaaatcc tgattttctt atcttcgtgt attttccgtc atcttttttg tatgaaattt
    57061 cgtattgcca actatttcca cgtttgacta atcttgccat aattgaatca gctctctttc
    57121 tctgatacaa taggaactat aaagaagccc attgtttggg ttttattatt ttttgcacat
    57181 cctttttgct tgccggcgca aggatgtgct atttgtcgtt ttaatggtat aattggatgt
    57241 gtattagatc atccaatcag aggtgtttat tatgattaaa gtgattttta aaagaataac
    57301 tgaacttcca gagtttagat tatttgttga ttattggtat gtctggtttt ttattgttgc
    57361 attgctgata ggtctagatg tcatattaga aagaaaactt aagtaagagt tgagttaata
    57421 aattgaattc ctaaatgaga tattttgtag cccatatttt gtttgccgac atcacctaat
    57481 actatggagc taatcgtttt ttctaacaga ccaaaatcag ataatctatt taagctacca
    57541 tctattagct cttgtgaata gccagacatg ctgatatttc cagtgaaatt ttcgtttcca
    57601 cgactcatta agcatgctaa caattgaatc tcaatcgatg ttaattgatt cagtacaaga
    57661 agataatact cctcgttatc ccatgaagcg ttgttaattc gaagtaaaca attcttataa
    57721 agatttttat acatatctat tttattttgt gcctttgatt tttctacctc gtcatttatc
    57781 ccttcgaata ttccaatgag ttggtccttg ttctctaagt tgaaaacatc ttctggaatt
    57841 tgatcgcgaa tgttttctaa tgattcgttt aaatttttgt agaatttctc gatgcgtttg
    57901 aaacgcttct cgtttttaga cccaaaataa agtgactgca caacgccacc aatataagga
    57961 attgctccaa taccgccttc aactattaat tcaagatatt cttttttggt tagtttttca
    58021 ttgctattat ccatagttac ctcctatttt gtcatttcct ttacaagcat tgtgactcct
    58081 gatggcactc ttttgtatag gttttattat tgtgcacaat ctaactctta gtggggcgtg
    58141 gttcatttat tttttactta ctttactatt aatcatatcc cattgagcat aggatttttc
    58201 tatgttcgat cctcttgaac taatttctcc tgtaaaagtt actttatcgc cctttgtaaa
    58261 ggtatcagca ttgtttaagt ccttcgcaaa aattacatat gaaactttag gtgttgcaga
    58321 aatatcattc caacttttat catcgtactc tttgtctgct ataatggcta ttctagtagt
    58381 cattgattcg ataacagttc cttcaaaagt atatgtttta ccactaatat atttagtaaa
    58441 agctgtattt tgctcagtag ctggagtaat agaataataa ctatctaaga aagcacccca
    58501 gtcatgatta tttgtctcta aaaattcttc aaattgagtt aggcttttat tgctgctgtt
    58561 agatgtagta gtttgagtat cagatgatga gctttcctgt ttgttattgt catttgcctc
    58621 gtttgatgag gaaagcgaga aacctacggt agtaaaaata atcccgataa taaggagaat
    58681 cataggtgtt ttcttatttg tttttttgat aaatcttaca attaacatga ttaaacctat
    58741 aataaataaa acaataccag taaaaccaaa tataccgtcc attaatagat cctctttctc
    58801 ttttgatatt gaatattata aaccaagcaa ttcttttttc ttaaaatcga attcttcttg
    58861 attaataatc ccttcatcta acaactcttt atacttttta atttcatctg cacttgaaat
    58921 ttgtgtttct ttggaattat tatctaatat agattgttgc ttctctatat atttttttaa
    58981 attttccatt tcattccaat atttttttgt aaacataacg gtgttttcat ctttcgtggc
    59041 cgctaatata cctccgcgac tttcgtttcc gcctagcaag gtaaattgta tataaccatt
    59101 actcatacct ggttttttta attgtacggc tgatatattt ttaaagggga tagttttttc
    59161 tcctttaata ccttgattaa ctaaatttat aaatcctttt ctaataattg aaatagaatc
    59221 atcagttact ttaatcaagg tttttcctgg agattttatt aaaatctctt tttccatttt
    59281 ttcttcctca tttctgtgat atgatttttt ttgtagaagc tcatatatga ggaaagagcc
    59341 cgtgtt
//
LOCUS       DAZULG010000003        57380 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000003 DAZULG010000000
VERSION     DAZULG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.003"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..1607)
                     /locus_tag="ACG9CY_000125"
     CDS             complement(39..1607)
                     /locus_tag="ACG9CY_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS4936673.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1611..2021)
                     /locus_tag="ACG9CY_000126"
     CDS             complement(1611..2021)
                     /locus_tag="ACG9CY_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4936674.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2018..2941)
                     /locus_tag="ACG9CY_000127"
     CDS             complement(2018..2941)
                     /locus_tag="ACG9CY_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS4936675.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2938..3816)
                     /locus_tag="ACG9CY_000128"
     CDS             complement(2938..3816)
                     /locus_tag="ACG9CY_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS4936676.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3803..5140)
                     /locus_tag="ACG9CY_000129"
     CDS             complement(3803..5140)
                     /locus_tag="ACG9CY_000129"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS4936677.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5143..6246)
                     /locus_tag="ACG9CY_000130"
     CDS             complement(5143..6246)
                     /locus_tag="ACG9CY_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4936678.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6320..7828)
                     /locus_tag="ACG9CY_000131"
     CDS             complement(6320..7828)
                     /locus_tag="ACG9CY_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4936679.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7794..8486)
                     /locus_tag="ACG9CY_000132"
     CDS             complement(7794..8486)
                     /locus_tag="ACG9CY_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4936680.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8508..9482)
                     /locus_tag="ACG9CY_000133"
     CDS             complement(8508..9482)
                     /locus_tag="ACG9CY_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4936681.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9782..10654
                     /locus_tag="ACG9CY_000134"
     CDS             9782..10654
                     /locus_tag="ACG9CY_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4936682.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11112..13310)
                     /locus_tag="ACG9CY_000135"
     CDS             complement(11112..13310)
                     /locus_tag="ACG9CY_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4936683.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13542..14534
                     /locus_tag="ACG9CY_000136"
     CDS             13542..14534
                     /locus_tag="ACG9CY_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4936684.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14594..16015
                     /locus_tag="ACG9CY_000137"
     CDS             14594..16015
                     /locus_tag="ACG9CY_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS4936685.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16008..17507
                     /locus_tag="ACG9CY_000138"
     CDS             16008..17507
                     /locus_tag="ACG9CY_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS4936686.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17546..18940
                     /gene="uxaC"
                     /locus_tag="ACG9CY_000139"
     CDS             17546..18940
                     /gene="uxaC"
                     /locus_tag="ACG9CY_000139"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4936687.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19099..19782
                     /locus_tag="ACG9CY_000140"
     CDS             19099..19782
                     /locus_tag="ACG9CY_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS4936688.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19784..20620
                     /locus_tag="ACG9CY_000141"
     CDS             19784..20620
                     /locus_tag="ACG9CY_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4936689.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20631..22385
                     /locus_tag="ACG9CY_000142"
     CDS             20631..22385
                     /locus_tag="ACG9CY_000142"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4936690.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22661..23002)
                     /locus_tag="ACG9CY_000143"
     CDS             complement(22661..23002)
                     /locus_tag="ACG9CY_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4936691.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23070..25241)
                     /locus_tag="ACG9CY_000144"
     CDS             complement(23070..25241)
                     /locus_tag="ACG9CY_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4936692.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25452..26309
                     /locus_tag="ACG9CY_000145"
     CDS             25452..26309
                     /locus_tag="ACG9CY_000145"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4936693.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26380..27180)
                     /locus_tag="ACG9CY_000146"
     CDS             complement(26380..27180)
                     /locus_tag="ACG9CY_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4936694.1"
                     /translation="MTIKKKLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGID
                     KTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNS
                     CIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAA
                     GPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLL
                     QKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
     gene            complement(27177..28136)
                     /locus_tag="ACG9CY_000147"
     CDS             complement(27177..28136)
                     /locus_tag="ACG9CY_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4936695.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(28287..30989)
                     /locus_tag="ACG9CY_000148"
     CDS             complement(28287..30989)
                     /locus_tag="ACG9CY_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4936696.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(31002..32291)
                     /locus_tag="ACG9CY_000149"
     CDS             complement(31002..32291)
                     /locus_tag="ACG9CY_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS4936697.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            32441..33487
                     /locus_tag="ACG9CY_000150"
     CDS             32441..33487
                     /locus_tag="ACG9CY_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4936698.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            33489..35642
                     /locus_tag="ACG9CY_000151"
     CDS             33489..35642
                     /locus_tag="ACG9CY_000151"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS4936699.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(36115..37566)
                     /locus_tag="ACG9CY_000152"
     CDS             complement(36115..37566)
                     /locus_tag="ACG9CY_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4936700.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(37563..39287)
                     /locus_tag="ACG9CY_000153"
     CDS             complement(37563..39287)
                     /locus_tag="ACG9CY_000153"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4936701.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKKQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(39280..39894)
                     /locus_tag="ACG9CY_000154"
     CDS             complement(39280..39894)
                     /locus_tag="ACG9CY_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4936702.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(40239..41696)
                     /locus_tag="ACG9CY_000155"
     CDS             complement(40239..41696)
                     /locus_tag="ACG9CY_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4936703.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(41737..42657)
                     /locus_tag="ACG9CY_000156"
     CDS             complement(41737..42657)
                     /locus_tag="ACG9CY_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4936704.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(42669..43616)
                     /locus_tag="ACG9CY_000157"
     CDS             complement(42669..43616)
                     /locus_tag="ACG9CY_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4936705.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(44095..44814)
                     /locus_tag="ACG9CY_000158"
     CDS             complement(44095..44814)
                     /locus_tag="ACG9CY_000158"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4936706.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(44863..46509)
                     /locus_tag="ACG9CY_000159"
     CDS             complement(44863..46509)
                     /locus_tag="ACG9CY_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS4936707.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            46688..47278
                     /locus_tag="ACG9CY_000160"
     CDS             46688..47278
                     /locus_tag="ACG9CY_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4936708.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            47370..48875
                     /locus_tag="ACG9CY_000161"
     CDS             47370..48875
                     /locus_tag="ACG9CY_000161"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS4936709.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(48959..50311)
                     /gene="celB"
                     /locus_tag="ACG9CY_000162"
     CDS             complement(48959..50311)
                     /gene="celB"
                     /locus_tag="ACG9CY_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4936710.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(50311..50829)
                     /locus_tag="ACG9CY_000163"
     CDS             complement(50311..50829)
                     /locus_tag="ACG9CY_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936711.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(50845..51171)
                     /locus_tag="ACG9CY_000164"
     CDS             complement(50845..51171)
                     /locus_tag="ACG9CY_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS4936712.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(51184..53163)
                     /locus_tag="ACG9CY_000165"
     CDS             complement(51184..53163)
                     /locus_tag="ACG9CY_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4936713.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(53184..53498)
                     /locus_tag="ACG9CY_000166"
     CDS             complement(53184..53498)
                     /locus_tag="ACG9CY_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS4936714.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            53666..53959
                     /locus_tag="ACG9CY_000167"
     CDS             53666..53959
                     /locus_tag="ACG9CY_000167"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS4936715.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(54037..55149)
                     /locus_tag="ACG9CY_000168"
     CDS             complement(54037..55149)
                     /locus_tag="ACG9CY_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4936716.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            55302..56150
                     /locus_tag="ACG9CY_000169"
                     /pseudo
     CDS             55302..56150
                     /locus_tag="ACG9CY_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(56503..>57380)
                     /locus_tag="ACG9CY_000170"
     CDS             complement(56503..>57380)
                     /locus_tag="ACG9CY_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFS4936717.1"
                     /translation="DGKLAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVV
                     ELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGL
                     APGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPV
                     TFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGV
                     LLVFISIKFRKKRNI"
BASE COUNT        17094 a        11719 c        10103 g        18464 t
ORIGIN      
        1 aaaataagtt agtgaacctc atttattaaa gaattaaatt acagatgatc ttttccatca
       61 tacttgaaaa actcaaaatc agcataactt cctctttttt tatttagatc atgcactgct
      121 atcccgataa aatttccagt aaaccctcct gagagtatac acatgttttt ttgcggacct
      181 actttcttcc attcttcagt tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt
      241 acagtgactt tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc ttctatttgt
      301 gctactccgt tttctgattc tatcatacgt aggcattttc ccttgtcctc attccaagta
      361 acataacaat aaatataatt ggattcattt aagtacagta ccagtccagc catctgatta
      421 aaattattag gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga
      481 atagctaaca aatgatgttc gaaggtagat tgaagagatt caccggaaat cagacgtaaa
      541 aaacctggtc tacattttgt atcacaccaa tcagcatttg gcaaaatgcg tctggcattc
      601 cagttcttct ttagctctcc ttcaaatgta tccataaact gatgatcttt ttcttgttgt
      661 acctcttcat ttgtctcaat cattatttct tccatcggag ttgtctggct atctttaagt
      721 cttaaccaat cgtcggacgt ccaatacact tcttgaatag cggtttctct tcccaaaata
      781 gccgcaccgt ttaatggtct ggtacaaaga tatgtcatgt accatcttcc tgacggggtt
      841 tgtactaagc tcccatgtcc tgtacattgc aatgtagaag caggtttgtc actagctgtc
      901 agcataggaa aaccaggatc aagctcgtaa ggaccagtaa tattctttgc tcggcaaact
      961 gtaactgaat gtcctcttcc tgtaccgcct tctgccgtca tcaaataata atacccgtta
     1021 cgaaaataca aatgtggtgc ctccgttttc gctaattctg tacctgcaaa tattttatag
     1081 atgggaccat caagcttctt cttctctgca tcatattctt gtaatacgat accgctggat
     1141 ttattcggtg tgtccagacg ataatcccat agttcattga caagccattt ttttcccgtt
     1201 tcaagatcat ggaacaaaga aggatcaaag ccgttactat ttaaataaat tggttcggac
     1261 caaggacctg tgatctcaga tgcagtgatc aaataattgt ggacatcttt aaacggacgg
     1321 cctgcacttt tgacatctgt gtaaacaaga taaaacaatc catcagcata actgatctgt
     1381 ggcgcccata tacttccatt tgtaggattt ccttgcaaag atacttgagt ggtcaaaata
     1441 tccgttgtat gtgaccagtt gactaaattt ttcgactcat atactcggat tcctggaagc
     1501 cattcaaaag aggagacaat aatgtaatac cgctctttca ctcttaatat attaggatct
     1561 ggattgaatc ctcttaaaat aggatttttc actttgatag tctgcatgat tcaggctcct
     1621 tttctcattc ttactcgata taaactgaat gccccagctg cgaaataaaa aaatgcaaaa
     1681 ggaaacgcaa taaactgagt gccaatcaac aatatgaatc ctgttataaa atacaaacca
     1741 gtggcagttg ttaagtattt tcctttagca gtcaaaaaca atgctactgc taaaaaagca
     1801 agtgaaataa acaacacaac ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta
     1861 ttttctaaag acaaagttga atgatctaca gcaagcgact gataactttt tttaaactct
     1921 gacgcagaca tattacttaa agtaactgct aacccgccaa agaaaatcaa agatagactg
     1981 ccaccgacca ttcctagcaa tagttcccat tttcttttca tttctgaacc ccctgctttt
     2041 ctaaatgtat ccattcaggt cttttgatcg atgtttcata attgttatta cgttcagaga
     2101 atttgacagt ctgttcattt ttcttgtcat cccaattatc ccaaccagtt gggtgaacat
     2161 gcccacctat cttacacgct tcaaataaga catttgcata cggacgccac ggtcttccta
     2221 aatagacgtt tctagtatca ggttctgcag tcaaggtaca gctttcaaaa cgataccctg
     2281 ttttttgatt tttcggcgta gatgctgctg tgatgtatcc aggattttta ctggctaagc
     2341 tatggatatg acactgatga aaaacggcat ctgccccacc aaatatgaaa tccaccgttc
     2401 cttcaatcca gcaatattca taggtctgac gacaatactc aggattggca gaagccggtg
     2461 tacaaaaagg agtaccatct ttttgcaaat caggcaacgg acccgtgcat aaggtatcct
     2521 gataaccaga tagtcgacag ttttttaaag atacgcgatc tcctgaattg aataaagcaa
     2581 cagcctgtcc taccttttct cctggacctg ctgtattttc gatgtgaaga ttttctaata
     2641 cgacattcga tccttctaag aaaaaagtag cacttcggaa agtccctaac tggctaccgt
     2701 caagatactc ctgttttgca aaacgattac cggagataac tactgttcct gctcccacaa
     2761 gtttaaaatt ggataatctg ctttctatat attcatcata tcttccttct tttactatca
     2821 agacctttgg atggctatcc ggcaggcctg ccaaataatc gattccttct tggatagttt
     2881 caaaatccgt cggggtatct atagcattct ttccaattat cagtttgtct ttcatcctca
     2941 taaatgctcc cttatgaatt gtttccaaat atttctcatt tcttgtgttt ccatatgacc
     3001 gcctgttaaa gaaaaagaag aaaagttttc tggactaccc attgattgat actttttgtc
     3061 cagccctttt ctcagcttca gtacgccttc gtccaagcac atcgtatcgt tttttccaac
     3121 taagctaaga cgagggcgcg gagcgatttt ttcttggatt gctaacgttg tataagctgt
     3181 caatagattt ggcacatagt aataaaaccc atgatgatct aggcggcgat gtagcaaaag
     3241 tatttcgatg tccacctgtg ccgctagatc aatacaaact ttgacacgag aatccaatgc
     3301 tgtcacccac cagcttaata agcctcccat agaaagacca atcgttgcga gccgttgagt
     3361 atctgtgtct tcccttgtct ccaaataatc gatcaagctg atcacatcat aaatacgcat
     3421 tccccataac gtttttcctg ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc
     3481 tccgcgttct tcaaatcccc aagcatcaat cgaccaaaca gcatatccca ttcctgtcag
     3541 tacttctgca aaagatggag aagctaagta aggagcacca tggatcaatt cacttttccc
     3601 gactgaaaaa tctccgccgt gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt
     3661 ttctggataa gcatagaggg ctggtactgt ttccaattca tttaagtaca attgatagta
     3721 ttcaagcata aaaccgtttt gtttttctcg gtataatcgt tttccactag gggtatgtct
     3781 gctagatgga gcacctaaaa aatcattcaa ctgcataagc aaagttttta cgaacaacca
     3841 gttcatccat attttctgat tgatttgatt ggaatttctc aatactgatt tctttagtat
     3901 tttctaatag aaatgctggt ccctcgtgat tttcaatcga catctggtta aaggaaacct
     3961 tacccgcaaa gccaatataa aatccttgtc tgctcatttc ctctattcct gtcatcatag
     4021 caggaacacc gggaactgca tcttgggcca tcgagacatc gatattgtca aatgtgattt
     4081 cttgtatgaa ttgttccgct aatccataaa taaaacctgc agaagcatgg acattccttg
     4141 ccgtgatatt cgaaaaatgg atccttctga atgctggtgt ccgttcatct atcggataag
     4201 cctttttttc ccatacatac ggttctttcc ctcgcggacc acagaaataa taaagattca
     4261 gtataaatgg acacatcaca ttatccatga ctatattgga tactcgaata tcttcaacga
     4321 tcccgcctcg acctcgtcta gatttcaatc gtattcctcg atcagtttct tggaagatac
     4381 aatttgaaat ggtaatatta cgaatactac cactcatttc acttcctaat acgacaccgc
     4441 catgtccatg gaccatcgtg caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt
     4501 acgtatcttc agtccctgcc ttgattgcaa tacagtcatc tcccacgtca atatggcaat
     4561 tactaatacg cacatttttg catgattctg gatcaattcc gtctgtattt ggtgaatccg
     4621 ctgggttcaa gatcgtcaag ttatcaaacg ttgcattact gcaaagaatc gggttgatcg
     4681 tccaacttgg tgactggatc aatttaatat ctttcacagt aattcgatga cagttatgga
     4741 aactcatcaa tttaggacga ggataagcga gattatctgg ttcatttcta aaagtatgcc
     4801 accatttttt tccatttcca tctaatgtac cgaaaccagt gacagagatg ttctctacat
     4861 tttgagcata gatacaggaa gcataaactt ttcgatggac tccttcccaa cgtgaatgaa
     4921 caacaggata atcttttgga tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa
     4981 gttcgacatt gctttttaaa aataaagctc ctgttagaaa ttcacctgct ggaacaacta
     5041 cggtcccgcc tccgtcactt gctgcagcat caatggcttg ttgtattgct tcagtattca
     5101 attcatcaat tgatgcgcca aatgtcaaaa tatcatacat atttataatg cctccacttc
     5161 aatcaatgct tgcaagaatg caccaacgcc tttttgatca ttacaaataa ttggttcact
     5221 gatgtagtaa gcatatgaac catctcgctg atctgctccg cctaaccctg ctacttgaca
     5281 gtttttattc aagttcagcc agccttcttt tgtttccaga acaaattcat caagtaatcc
     5341 ttgatgtgat ttcttcactt gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat
     5401 tcctttagcc attgcgtaaa caaacatact actgccggaa gcttccagat aatttccttt
     5461 ccgttttgtt tctgttgtta cttgatacca tgtcccgctg ttttcttctt gatatggaag
     5521 taatgcagcc agtgtctttt ctagaatttc tttcagcaaa gaacaatctg tttcttcatg
     5581 aagtaattca taggtatctg ctgcagccat caaataccag ccaatcgatc ggctccaaaa
     5641 atgatgggat agacctgttt ttggatcagc ccatggttgg accttctttt catcccatgc
     5701 atgaatcagc aaatttgttt ttggatcgaa tagatgttta taactgattt caaactgtct
     5761 caaaacatct tggtaatctt tttcttcact aaaattctgg ataaattcag cataaaaagg
     5821 agaacccata tataagccat ccaaccaaat ttgataagga taaatttctt tatgccaaaa
     5881 agctccttcg gatgtgcgtg gatggtttgc caactgctgg gaaaggagtt ctgctgcttt
     5941 tttatatttt tcttcgcctg tttctttgta caataaaaag aaaagctttc ctgtatttac
     6001 atggtcgata ttgtattctt caacgctgta gccacggatc gttccatctt cttgcacgaa
     6061 atagtcgata ttgtctttta tgaaatcaaa ataaattttt tcttgggttc gttcccaaag
     6121 taattggaac cctttaagta cgacgccata gtcataagac cattttcctt tataccactt
     6181 ttcttcatat aagcgaggtg ttctttccat gatacttttt gctaatttct ttgaccagtt
     6241 ttccattatt tgctctcctt taaaaattaa aataggattt gggacaaata ccaagaagta
     6301 tttgtcccaa tcgtctatat tactgtgccg ctgctttgta tgcttcctcg tactcttgtg
     6361 tgattcgatc gcctccttgt gctttccagt cttctacagc ttttttgaat ccatcttcgt
     6421 caagctgtcc catgatgtat tgcaccgtag catcaatgac gattttttca atttctgatc
     6481 ctacttcatt gttcgtcggt gaatccaatg gcacagaagg atccattaca gcaaattctt
     6541 cattttcttt gatcaattga ttagagaatt ctttttcagg gtctgtatct ttgaaagaga
     6601 acgtcacttc actaggacga gagcttgata acggttgcac ctcttgttgc caaagatcca
     6661 tatcgataaa ttcgatagaa ccatcatcat tcaattcata atgcgttcct tcgataccgc
     6721 cagtcatcaa tttatacaca tcttcttcta aaagatcatc aacaaattga agtaatttct
     6781 tcaattgtgc ttcgtctttt acttgagatt tgggaaatgc tagcaagccg ccaataccat
     6841 ttccttctga ccaaacatga taaccgccgc cttcaccatc tgagatcttg tttacaggca
     6901 ctaattccat atcatcttga ataccttgac ccatcgtctt cagattcttg atatccacca
     6961 ttccagtgta gattccagtt cttccttgtg caaattgttc ttgctgatca gtcttttggg
     7021 taactgcaaa gtcttgtgcc aaatagccat tctcaaatag atctcttgaa aaattcaaag
     7081 ctttcatata ctcttctgta tcaaattctg gagtgaattt gccattatcg tctacagccc
     7141 agccatttgg tgctccaaaa taagaagtga aaagtttaaa gctggtataa cgaatatcac
     7201 taggcgcacg atcgccaaaa ccaactgtgt cgtccacacc atttccatca ggatcgtttt
     7261 ctgtaaattg tttagctact tcataaagct catccatggt agttggaaca tctagtccta
     7321 gattatccaa ccagtctttt cgaatgacta agccagcacg agcgtagtct ttttgtatcg
     7381 gtacgccgta caaggcaccg tcgatactcg cagcttcgat tctatcttca gaaatttgac
     7441 ttaaattttt atagtctttt aaatattctg atacatccca gaatacgcct gattttaacg
     7501 cattgcggac agaagagttt ttcaacatcg caatttgaag tgtaacgata tctcctagtt
     7561 catcagaagc caatgcagtt gttaatcttt cttcttttga agcatcagga atccagttga
     7621 atgtcagttc cgtatcggtt ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg
     7681 aagcatgatg caaaatattt aaccaagtga ttttagcttt tccatcttct gtctctgaag
     7741 aactgttact tgaactgctg ccgcatgcgg caagtgctag aattcctgca aaactaagac
     7801 ctaattttac gagcgttgct tttttcataa attttctcct tcactgtttt aataatattt
     7861 ttgttttctt ctgttttttc tgtatatgag ttcttccatt ccaaatggca aaatatagaa
     7921 tttgctgtcc acgtgactgc ggaaaagaag agactgccac cgataaaggt taaaacacct
     7981 ggcattgttt ggatgatcat cagataaatc cctaccatac ttaatatcaa cactaatgag
     8041 taatgaagat gtgagaatcc gaataaaaat gccattttca ttttgaaaaa atatcctttc
     8101 caatcataat gagccatgat tgggaaaata tagagtgctg agagcacata tatgaacgca
     8161 gcaatataca aagcgatcct aagataccaa ttgtgtatga aagaaagatc cacaagtaaa
     8221 acaatcccga tggcgccata tatccagctc atcagcaagg tttcacgata gttttcttta
     8281 aaatatgtga agtacgtttt cgtcaaagga aacgtttctt ttgagcgcag ccattttcta
     8341 atcactgcaa ccatcgcata cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg
     8401 actaaaccgc cccacagaaa attcaaataa accaactgta aaatacgatt tagccttccg
     8461 tttattttat ctaatgactg cagcatatct ttccctctct ttctatctta gtaaacgccg
     8521 tcttctccta gctttttggc aatcttatta gctaaaatta ccataaacaa tcctacgacc
     8581 cctttaaaca atcccattgc tgtactgaaa cttaactgtc cattcttcaa tccagctgta
     8641 taaatatagg tgtcgaaaat ttctgcaacg cttcgattca atgaattcaa taacaagtac
     8701 atatgctcga accctaagtc taatgtctga ccgattttta gaatcagcaa ggtaatgatt
     8761 actggacgaa ttgccggcaa tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc
     8821 atttctgcag cttcatacaa ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc
     8881 gtagaccagc ccaactcttt ccagatcact tgtccgatat atactgtgcg tgtccattca
     8941 ggggatgtta gaaaactgat tttttcaaat cccattgttg caaggaattc attcaatacc
     9001 cctccgtcaa cattcaaaaa tacataagta atcgaaacaa tgattaccca agacatgaag
     9061 tgagggatgt aaatgatcgt ttgaatcccg tttttcaatc gtttgttcgt tacttcattt
     9121 aatagtaaag acaaaatgat tggcatcgga aaaaacacga agatatttaa tccgaacaag
     9181 atcaacgtat ttcttaataa tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt
     9241 ttgaaaccga cccaaggact tcccattatt cctaaaaatg gttgataatc ttgaaaagca
     9301 ataacgagtc cccccatagg aagataccta aaaatcaaga aataaaggac tcccggcaga
     9361 atcatcacat ataaaaattt attcgttttg attcctgcaa ttattttttt tcgtcttctg
     9421 agcttttctt cttttttcga tagcgtcagt tgaaatggtt cttgtttctt tacactttcc
     9481 ataagcccct ccttagtttt gtgaatgttt gctgcttatg acttcatgat agataactat
     9541 ctgaattacg tatataatca tttttaccgt aagtaaaaac gcttacatag caatgatttt
     9601 tgtgctatac aaaacgataa agtgtatttt ttcacaaata aactacgcat agcaaacctt
     9661 ctattttttt ctatctgaca cttagcatag gaaatgatta tatacgtaat ctttcaccga
     9721 tgataaattg aacatataga aacagaaaca tttaatttat tttgaggagg agcctatcta
     9781 tatgaaaaag aaagtctcta taggagaaag aagttttaca cttttcaaca atacgtttct
     9841 aattttgcta gctttgatct gtatcgttcc ttttttaaat atcatcgcta catcttttgc
     9901 ttctacacaa gaagtcgtcg cgaaaaagtt tatccttttt cctactacct tttctttaga
     9961 cgcgtatcgc tacattcttt ctacgccgac catttttaga gcacttgctg tttcgattgg
    10021 tgttacaggt gtcggaacga tcgtcagtat gtgtgcaact tcactgatgg cctacggtct
    10081 ttccagaaag tatttgtttg gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt
    10141 gttcagcggg gggatgattc caacgttctt agttgttcgt tctctagggc tggtcaattc
    10201 ttactgggcc atgatcctgc ctgtggctgt taatgcaatg aatatgatca ttatgagaaa
    10261 ctttttccaa gccttgccgg atagtttgga agaatcagca aaaatggatg gttgcacaga
    10321 ttttggtgta tttttcaaaa ttatgctgcc attagcgtta ccgtctattg ctactatctc
    10381 gcttttttat gctgttactt actggaacac ttatatgact gctattttgt atatcaacga
    10441 ttcatctaaa tggccgatac agattctttt acgccaaatc gtcattgtat caagtgggat
    10501 gcaagcagaa agtagtgccg ttgatgtgat tccaccagca caaacagtta aaatggctgt
    10561 tatcgttatt gcaacagtgc ctatgctgat tgtttatcct tttgttcaga aatattttgt
    10621 caaaggagca ctcgtaggtt ctgtcaaagg gtgattatgt actgaacgca aaatggtagt
    10681 tgtgtcagaa gtggtcagct tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa
    10741 tttttgtgaa atttcggctt atttttcgaa gaatctactc ctggaacacc gtgtatcaga
    10801 ggttgtgaaa gagacgttca gccttgagcg atgataaaaa ctctcacaaa cattgcgacg
    10861 agtaacccac aggagcaata agaagcgatt ttgaaaaata gcttcccata tttcacaaaa
    10921 ttatgcctta ttgtcgatag cctgtctctt gaacgccatt tattcgtaaa aaagaactgt
    10981 gacaagactt ttgtcacagt tctttttgcg tgcttagcct tgaaatttaa ctatccataa
    11041 atacaaatac tagggttatt ctaacttctt ttattgcttg aatcagtcca caagaaaaac
    11101 agcctcttga ttcacaaata tttttttcga tattctcccg gcgtaatcga ttccttcttt
    11161 ttaaagaaac gaataaaatt ttgcggattt cgatattgca atctttctgc aatctctttt
    11221 acggagaggt tagtctcttt caaccatttt tttgctacct ctaaacgatg attttgaaca
    11281 aaatcaccaa atttttctcc tgtttccttt ttaaatacat tacttaaata gttagggtta
    11341 taatgcaacc tatcagcgat cacatctaaa gacagatcac gatcatattc tgtgtagacg
    11401 atatgcataa tattctccga caaattttta aattcatgat cagtgattgc ttgagagcat
    11461 tctgtgattg gcagaactaa ttgttctaaa acaagttttt ctagttcttt gggatgataa
    11521 ttgttcagtg cttttacata aatttgtttc atgttatcaa agttttttga atctactccc
    11581 aataactgtc ctaattgaac gagttcattg atcaagcgga ccgaaactac ttcccgactc
    11641 aaaggatttt tattttgtgc aaacaatgca tcaatcaatt catgtactaa attcttcact
    11701 tcctgatttc cagaacgaat ggcttcaaac aatcgatttt gttcttctat cggatatcta
    11761 ataagtgttt tttcatgagc agcaggaacg atttcctgat aaaaaataat tgaacttgga
    11821 ccagtattta cacgataata cagggcttct tttgcccgat caaccgattg cttgctttca
    11881 ttcagcgttt cgaaccggtt actgatgcct atactgatag ttaaattcaa gtattctttt
    11941 acacgttttt gaatgagtcg gcaatactcc atgacttttt tgcttgcttc tgcatcatct
    12001 ttatccattc gataaatagt ggcttgcatt tcttcattca aaacgatcgg cagcattcga
    12061 ttttcctttg gcacaatttc cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc
    12121 tccccaccgt gactatcttc taacagatcg atttgaacca aagctgtata atagcagtca
    12181 ttttgagaac tataaccaaa ttgctgcaac cgctgatcta gttctcgcct ttctactcga
    12241 tttctaaata aactaagtac aaatagcgtt tccagctgtg gcttttgggt aactaagtgt
    12301 gcacttaacg cttctttttc tcctatgatc cgactgactg attgagcgac caaatctagt
    12361 tctttcccac tgaatccttt tgatttaaga ttcagacgtt cctgtatttg agaaatcgga
    12421 cgggcaaatc tgtccgaaaa taagtaggaa agaagaccaa tgagtcctac cagtgttaaa
    12481 gacactgcaa ctagcccgat tctcagattg cggatcattg cgccaattgc actagagtca
    12541 acttctaatc tgtaaatcca attattataa tctgatttca gtactatata cgttttatct
    12601 ttttctctta tcactttcag ctgattgtaa tctagatcca cgtccgttaa tacagttggt
    12661 tgctctttat tttttactgt ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa
    12721 agtatgtctt ctccactttc accaacaact tgttctattg tatgtttctc tatcgtagca
    12781 attcccaatg catatttttc tttttcaaac attggcaaag aaatcaccat ttcgattcct
    12841 ttgtttttct tttgccaaaa taaattattt ttgttattta tgtacttctg ttgatacttt
    12901 tcaatttcct cttcggtcag ttgactcaac gatccattga ttactcccca tttaccagcc
    12961 aaactgacta aatcataatt attatcatcc ataacaatcg tttctataaa atttagttct
    13021 ttcctgattt cagaataagt ggagtaatct gtatatgtca gcggatgctt gaatatgtcc
    13081 gagaaatttt tggtcgaacc atacgtagaa aacgaatatt caatcgttct aattttccat
    13141 tcaacgttag ttttaacttg cctgagcaaa ttctttttgt ctgttaaata actcgattcg
    13201 atcgattcat aggtattctg gtaaatgaat aaactaaatc ctatgacaag tacggctcct
    13261 aataaaaaca tcggtaaaaa tattttataa aatactgtac ttttcttcat tttcttcctc
    13321 tcctcctttt aaaataaaag gctttcattt ttctgtaaaa ttgatactca tctaattaaa
    13381 tgagcattcc ccgctttcaa gaatagcata attaaaaata aaaatccaag taatttttaa
    13441 cgtgtgcaat cttttcatat caaagatcta ttgacttatc gtagttatcg taatatgatg
    13501 aatgcgttaa caaaaagtga aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg
    13561 tcgctaaagc agctggcgtt tctcatacga cagtttctcg tgctttgaat aacagcagtt
    13621 taataaaaaa tgaaacaaaa caaaaaatac gacaactagc taaggaaatg aattattcgc
    13681 caaactatag tgcaaaaggt ttagtgaatc aaaaaaaata tctaatcgga ctatttttct
    13741 caagtttgca taaaggaact tcatcaaatt tcctagcaga ttccattgca ggcatccatt
    13801 cagtattaga cacaaattac agtttatctg ttgaaagtat cgatgttgtt gatttaacag
    13861 aaatcaacct gcaacgctac gatggactga tcgttatgag tcaaagtgat gatgatcagc
    13921 ctttcatcga ctatctcaaa gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca
    13981 atgatccagc ggttatcaac gtcactgccg atgatgcggc cggtgttatg caggcagtag
    14041 attacggtat tggatgcgga catacgaaga tcgcttatat tggcggaaag aaaaattttc
    14101 gttcgacaaa agaacgggaa aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg
    14161 tcaatccagc ctatttttta acaggagatt acagcttaga aagcggcctc caaaacatgg
    14221 aatacctatt atcactttct gacctgccta ctctggtttt ttgcggcaac gacgacatag
    14281 cgattggtgc attgagagct gtacatttag ctggcctccg tgttccccag aacatttctc
    14341 tgatcgggtt cgatgattct ccagtcgtct cctatctcaa tccgccgctt accaccgtac
    14401 gtaagccttt gaaagaaatg tgtcaagaag gaacaaagct tttactagat ttgatcgaag
    14461 gcaggacgat cgaacaatct aaaattgagc tctccacctt gttgatcctt cgtgagtcga
    14521 tcaattactt gtaaactaca agtaatttta tataaaaatg ttaacgtgtg cataacacag
    14581 aaaggaagtt tatatgaaaa aactgacaaa agaattagtg aaaaaaaacg aagcacctgt
    14641 aaaagttctt cagtttggtg aaggcaattt tatgcgtgga tttattgatt ggcagattca
    14701 gcagctgaat aaccaacaat tgtttaaagg taatgttgca atcattcaac ctttagaaca
    14761 aggtcttgga aatatgatga accagcaaga taatttatat acggtcattt tacaaggact
    14821 cagcaatggc gaaatcgttg atacttccga gattatcacc gtagtagaac aaatgatcaa
    14881 tccttatgat gaatgggatg cttatctatc tctagcagaa aatgacgact tggcctatat
    14941 cgtctccaat acaacagagg caggaatcca attcgtggag actgataatt taacagatac
    15001 tcccccacag agcctcccgg gcaaactgac agccttttta taccgacgtt tcaaattaaa
    15061 caaagctggc tttaccatca ttccttgcga actgattgac agaaacggcg aacgtctaaa
    15121 agaaatcatt ttacgctacg cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt
    15181 agaaaaagaa aatgttttct gttgtagttt agttgatcgc atcgtcccag gttatcctcg
    15241 cgaagatgca ggtgttttca atcaacggca taactatgaa gatcaattga tggtcaaagc
    15301 agaaccattc atgctttggg tgatcgaagg gcctaaagaa ttggagaatc aactaccatt
    15361 caaacaggct ggcttgaatg tcatcatcac agatgatatg actccttatc gtgaacgtaa
    15421 ggtgcatttg cttaatggac cgcatacagc gatggtccct cttgctttgt tggcggaatt
    15481 ggaaactgta gaagaagtaa tgaaagatcc tttattctcc gcatatgttg atcagttatt
    15541 caatctagaa ttgattccta tgctttcttt acctaaagat gaattagcca tttatgctga
    15601 ccaaatcaaa gaacgtttct tgaatccttt tgcccatcac aaattagaag cgatttcatt
    15661 aaatagtgtt tcaaagttca gcacccgtct gcttccagtg ttcaagaagt atatcgaaga
    15721 acaaaatcag gtaccaccgt taatcactgt ttcactagct gcgctacttc ttatgtatcg
    15781 aggcgatcaa gtaaagccac atgatgatga aaagacgata agcgaattta ctgatgcttg
    15841 gtctgataat ggaacagcta ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga
    15901 tttgtctcaa ataccgaatg tgacagatac agttcaagaa atagctgcaa agatcgatca
    15961 aaaaggtgtc cggtcagtat tacaagaaat gaaaggaacg atggtccatg cttgaaaccc
    16021 tgcaaccaac aatgaaatta aatcgacgag acagtgtggt cgtcgcatta acccctattc
    16081 caaaacaaac acaactgacg attgatcagc aaacgatcac cactcttgaa gatatcccgc
    16141 aaggtcataa gattgcattg ggtgatttga aagctggaga caacgtgatc aaatatggct
    16201 atccaatcgg tcatgtgacg acagacgtaa ctgccggtca atggctgcac actcataatg
    16261 taaaaaccaa tctttctggt gaattggatt atcgatatga aaaagatgtc catccaagtc
    16321 attacctttt tgagaaccga acatttcaag gttacttgcg aaaaaatgga aaagtaggga
    16381 ttcgtaatga tttgttttta gtacctaccg ttgggtgtgt gaatggcatt gctgaactga
    16441 tcgtcaaaca gttcaaggaa aaacatccag atctgggaca gtttgaccat attacgattt
    16501 taaagcatcc ttatggctgt tcgcagcttg gaaaagatca tcaaaatact cgagaaattt
    16561 tagcggacgc agtaaatcat cccaatgccg gaggagtttt agtatttgga ttaggttgtg
    16621 agaacaatac agttcctgaa ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga
    16681 aatttctagt ggctcaagat gtttacgatg agatcgaaca aggtgtggct ctattagaag
    16741 agcttttaac agcggcggaa aatgaccagc gcatatcagt cccgctctct aagttgaatg
    16801 tcggtttgaa atgcggcggt tctgatggtc tatcgggaat aactgctaat cctttacttg
    16861 gtgcattttc tgactacttg attgctcaag gaggcagcac gatcttaaca gaagtgccag
    16921 aaatgtttgg cgcagaacaa gtattgatgg cacgagcgga aaatgaagaa gtttttgact
    16981 caatcgttga cttgatcaat gacttcaaac agtatttctt gtcttacggg gaaccagttt
    17041 acgaaaaccc atctcctggt aataaagcag gtgggatcac tacattagaa gataagtctt
    17101 taggctgcac gcaaaaatca ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa
    17161 aaatcagaaa accagggctt agtttattgc aagctcccgg gaatgatctt gtagctgcct
    17221 ctgctcttgc ttcatccgat tgccagttgg tcttgtttac cacaggaaga gggactccgt
    17281 ttggctcata tgtcccaacg gtcaaggtat ctacgaatac aactcttttt gaacgcaaag
    17341 gtcattggat ggattttaat gcaggcgtct tattagaaca accaatggaa gtaatcttag
    17401 aatcgtttgt tcaaaaaatc atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa
    17461 atgacgttcg agaaattgcc atttttaaaa acggagtaac actttgattt tcataaaaca
    17521 gtataaaaag aaagaaggaa gatttatgtt tttatctgat gattttttat taaaagacga
    17581 ttgggcgaaa aagctttacc attcctacgc aaaaaacatg cccattattg attatcactg
    17641 tcacttagat ccaaaagaaa tttacgaaaa caacaatttc agcaatctga cagaagcttg
    17701 gttaggaggc gatcattaca aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa
    17761 aatcacagga aatgcttctg attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa
    17821 aattatcgga aatccgcttt acagttggtc acatcttgaa ttgaagcgtt tcttcggtat
    17881 tgacttgttg ataaatgaag aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt
    17941 ggcagctcct gattttcgcc gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg
    18001 tacaacagat gatcccgcag acgacttgca ctatcatcag ttgcttgctc atgaagaacc
    18061 agatttgaag gttctacctt cattccgtcc agataaagca ctcaatattg aaaaagaagg
    18121 atttaatgca tgggtcacac tactggaaga agcagctgac caaacgatca ccacctatca
    18181 agaactgata gaagtcttag ggcagcggat agaatacttc catcaacatg gatgtcgtgt
    18241 ttccgaccac gctttagaca tcttacgcta taaagaagcg gatgaaacag tattagaaga
    18301 gatttttcaa aaagcacgag ctaacaaact attatcttca gatgaaatcg atgcttatcg
    18361 tacagaaaca ctgatacatt tgacccactt ctatcattct cataattgga cgatgcagct
    18421 gcacatccat gcttatcgta actgtaatac acaaatgttg gaaaaattag ggcccgatac
    18481 cggctatgat ggaatgaatg atctttcttt gactattcct ctgcaaaaat tattgaaccg
    18541 tgcagaagaa acggatcagt tgccaaagac gatcctttat tcattgaatc cgaatgacta
    18601 tcctgcgatt ttagctttga tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt
    18661 aggttctgga tggtggtaca acgatactcg agctggtatg cgcgatcaat tgacacaatt
    18721 tgctgatggt agtgctttgg ggaattttgt cggcatgctg acagattcaa gaagtttcct
    18781 gtcctacact agacatgaat atttccgtcg tgttctttgt gaatttttag gcgaaatggt
    18841 catgcgagga gaagcacctg aagatgaaca acttttagga agtctagttc aagcgattag
    18901 ttatacaaat gctaaaaatt actttggctt ttttgcctaa ttgattaata gtaacagtaa
    18961 cgaatcaaaa acaggatagt gaaataacgg aatccgttta tccacgtttt agagaagaag
    19021 acggaccaaa tttgctccgt cttcttctct tgctgttctg cttcattgcc tataaacctt
    19081 tatgaaagaa gagataacat gcctactatc catattgccg gtgattctac tgcggcagaa
    19141 aaagatgaaa acaagcgtcc cgagacaggt tggggagaaa aaatctctgc ttatttttca
    19201 cccgagatcc agatcaataa ccaagcaaag aatggacgca gcaccaaatc atttatccag
    19261 gaaggaaggt tagcccgtct agcttcacag tttcagccga atgatttctt atttgtacaa
    19321 tttggacata acgaccagaa aatccaagaa gaaagaggta cgcttccctt cggtagctat
    19381 ttaaaaaatc tgcagatatt ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta
    19441 ctgacatctg tgacgcgacg agattactta gaaaacggaa cattgaatcc agatatactt
    19501 ggagactatc caaaagcgat gcgtgcattt gctgagaaac accatattcc tcttttagat
    19561 gtgttctcaa gatcacaaga attgttccag accttctcaa aagaagagac aaaaaaattc
    19621 tacctgcatc ttatacctga tacttgtaaa aattacccag aaggactaaa agacaatacc
    19681 cactttagtc cagaaggcgc tgataaagtt gcccgaataa ttgttgaatt gataaaagaa
    19741 tcccgtctgc ctcttgctaa ttatttacaa aaaggagttt gagatgtttc cattaaattt
    19801 aggcatccgt gcccacgatt tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc
    19861 acagcttcac ttgtcccata tccaatttgc accacaaaaa gcatttcctg aacaacttaa
    19921 attaactgac atgacacttg ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa
    19981 tatcgaactt tccattttag gatgctatat caacctatcc agcaaaaatc aagaagttcg
    20041 tgcacaagca ttggcaactt tcaaacagca aattgctctg tgctctgcct atcaagccaa
    20101 gatgattgct acggaaaccg gtagtgtctc tgtcggttac acaaaagaaa attttactga
    20161 agaagcttat cagatcgcca gaaattctat cattgaatta gtcgctcatg cagaaaattt
    20221 tggcgtaact gttgctgttg aagcagggat caatcatcca atctataact atcagttagc
    20281 taaacgttta atcgatgaag tagcttcacc aaatttaaaa atcattttag attgtgcgaa
    20341 tttgatgcat aaagaaaatc atgctgatca ggaaaaagtg atccgaggtg ccttggacaa
    20401 tttacatggt tatattacag cgcttcatct caaagactat atcatggaaa atggaaagat
    20461 ccgcatggta cctgttggca agggctgttt agatttccgc ccggtactgc attatatcaa
    20521 atatgagcgt cctctaatgt acgccactct tgaagctact cctgagattt atgttcctga
    20581 agcaatagaa cacttgcagt ttatttacca agattgttaa ggagaagatt atggaggaac
    20641 gaatacagaa aagttatcaa aaatatcatg caaatgcttt tcagacattg ctgcatcttt
    20701 atcagggaaa ttattggcgt tttgccatgt caggactctt ttttattatc aaacatcttc
    20761 catcttggtt aatgccgatc gctattgcca atgtaatcaa tgcagcttca tcgaaagatt
    20821 cttctcagtt gcaggccatt tatttcaacg cttttttgat ggcttgtctg attggtcaaa
    20881 atatattgac gaattatttt catgtaaaat accatagcac ttcgatccgt caagtagaag
    20941 ctggactaag agcgaccctt atcaagaaaa tccaagaact atctattcct tatcaaaaag
    21001 aattgcaatc tggacgaatc cagtcaaaga tcatccgtga tgtagaagct atccaaacgt
    21061 tgtcctctca agtatttgtc agcggattaa atatcgctgt caatttattc gtagctttag
    21121 gtatcacgct ttataaaagt cctgttgttt tcatgttttt cttattgact gttcctctcg
    21181 ccgcattcgt tactctcttt ttccgtaaaa ggatcagaca gtccaactca gacttcaggc
    21241 aagaaatgga agagacttct gcaaaagtga tcgaagtgat cgaattagca ccgattgctc
    21301 gagctcatgg gttagaaaaa cacgaaacaa ctcgcttaaa taagcatttt actcaaatct
    21361 atgaaaaagg attccactta gatatcctgc aatctgtttt cggatcagtc agctgggcta
    21421 gttttcagat atttcagctg atttgtttga ttttcactgg gatattagct ttacaagggc
    21481 gcatccaacc cggcgatgtc gtgatgtatc agacgtattt cacgacgatc gtcaattccg
    21541 tttctggaat gatcacgctg attcctacga tttcaaaagg catggaatct atcacttcta
    21601 ttggcgaact gttgattgct ggcgatgtag aaacttatca cggtaagaaa aaaatccaac
    21661 atttggatgg aaattttcgt tttgaggatg tttcttttct ttatccggac tcactcaaac
    21721 cgattatcga tcatttttct ctagaggtga aagctggaga aacaattgct tttgttgggc
    21781 catctggctc tggaaaatct acactcctta acttattgat tggatttatc cagccgactt
    21841 caggaaaaat ttttctagat gatcagcctt tggatgaact ggatatgcga agtgtgcgaa
    21901 attatttagc cgttgttcct caaaccactt tgttgttctc cgcttcgatc aaagagaaca
    21961 tcacttatgg cttgaaaaac gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc
    22021 aactttcttc actgattgac cagcttcctg atggtctgga taccttagta ggcgaacatg
    22081 gcaacaagct gtctggtggc caaaagcaac gtatctccat tgcacgagcg ttgatacgtc
    22141 agccaaaaat cattttgcta gatgaagcaa cctcagcttt agataaccag tcagagaaaa
    22201 aaatccagca agccctgaat tacctgacag aaacacccac tacttttatt gtggctcatc
    22261 ggctttcaac gatcaaagag gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg
    22321 aaataggtac ttacgaaaaa ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg
    22381 aatgacaaaa aataggagct atgacaagac ttttgtcgta gctcctattt ctgaataaac
    22441 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg
    22501 ttttttgaaa ttgttccttg taactcagag ttaaacggta ggctcacgac ctcttggtaa
    22561 atggtgttca agagacagcc tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg
    22621 aacgtctctt tcacaccctc tgttgtttca taaaatagtt ttattcgctg taaaggtcgc
    22681 gaactggtgt catgatgata cggttcaagt cattgatgat tgtactgaag ccttgctctt
    22741 tttgcatcaa gtcattgatt acttcttctg attggatttt cacagccatt tcttgtgctt
    22801 tcattgcatc ttcgtcgctg aactcttgtc cactcatttg tttttcttgt aattcgattt
    22861 gcatcgcttg gaagtctttg aacagtttgt gtgcttcttc gtttgcttgt actttctcgt
    22921 acgcttcttt caacgctttg aattctggta attcacggat ctcacgttcg atttgattcg
    22981 cgctatcgta gatgtttgac atgttatgcc ctcttttctt ttagtagtta ctctctattg
    23041 taacatgggg ttattcgttt tcaaaggggc tactctcctt gatatttccg ccagaggtct
    23101 ttagccgctt caccaattcc ctgaacgcct tctttcacca tatcgccgaa gtttttcagc
    23161 ccttcttttg cttgttcacc atattgattc aagttatctt gccaatttcc agactctcca
    23221 ttagaatttc ctgtgtttcc aacttgatct gcagctacca cttgtcctcc tgtggcatag
    23281 gcatctgcta cactaaatcc tgtttttgaa gtatacggaa ggatgccttc tgccgctgat
    23341 ttgaagactt tcccgacaac atttccactt gtcccttcca aatagtgcag cttgctggat
    23401 tcttcgtaac ccatccatgt cgaaatcact acgtctggcg tgtaaccgat catccattgg
    23461 tcatttgctt ttgtggcatc gaaattagtt tctgtggttc ctgttttccc tgctatcgta
    23521 taacctgcag gattcgcagc agccgctgtt ccatttgaaa atgtaccaag aagcatgctg
    23581 gtcatttcat cggctgtttc ctttgatatg acctgttttg gttttttgtt tgtgttatcg
    23641 acgatcaccg ctccagtcga atcaacgatt ttcgtgataa gatgcggcgt atacatctga
    23701 ccatcattgg caaatgcact gtaagcaccc gccatgatca atggagatac tcctttttcc
    23761 agtccaccaa gggctaagcc ataatactta tcagattttg ctaaaggcag gccgaattct
    23821 tctgctttat catatccttt ttgtaaaccg atctcatgca aaagccatac agtaggcaaa
    23881 ttcaaacttt gtgcaacagc ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa
    23941 ttatgcgcat cgtaataact ttgcggttcg tctttcaaga tactggaagg cttgtatcca
    24001 gcttccagtg ctggtgtata tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc
    24061 atctgtgtag caaagctgaa tcctctaaag acatgatctc ctcttcgtcc tactagtgct
    24121 tgtacaccac ctgtattcgg atctaaagct actgaagcag attggaccat ggcaccatct
    24181 gaagcatttg ctgggaataa tgcactgttt tgataagtag cttctaattg cttttgataa
    24241 tcctgatcta aagctgtata gattttatag cctttattca tgacatcttc ttcatcaaga
    24301 ccatactcgt taactgcttc atcgatcact gcatcaaaat aataaggata acgataacca
    24361 gaatctgaat tatcgtatgt gtcattaaga tacgcgctga tatctgtctg gctttctgct
    24421 tgttcttgtt cttttgtgat ttttccgttt tcttccatta cacttagtac tgtgtttttc
    24481 cgatcattcg cattttttgg gtgatcgatc ggattataaa taccaggacc tttcagcatt
    24541 cctgccaaag tagcagcctc agacagcgta acattcgttg catctactcc aaaatacttt
    24601 ctggaagcgt cttggacacc ccataccccg ttgccaaaat aggcattgtt aagatacatg
    24661 gtcaagattt cttctttgct gtacttcttt tcgatctcga ttgccaaaaa taattccttc
    24721 gcttttcgat caaacgtttg gtctaatgtc agatacgcat ttttggcaag ctgctgagtg
    24781 atcgtacttc ctccgccccc tgatgttccg ccagacaaga ccattcggac tgctgcacgg
    24841 gcaatccctt tgatatcaaa cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt
    24901 actgcatctt ggatataggg agaaatcgcg tttaattcta catatgttcc tttttgggcg
    24961 tacagtgtgc cagcttcttc gtttttctta tcataaatga ctgtcgattg gcttaatcct
    25021 gattttaacg tttcgacatt tgcttgtttt gctaaaataa agaggtagat acttgtcact
    25081 aagacacaga tcaaaccaat cagcaaaatc aatttgttca catgatattt tttccagatg
    25141 cgctttcgcc atcgatggaa ttgtccgaga tatggtctga tccaacgcca aaaactgatc
    25201 aaagcagctt ttatcttttc taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta
    25261 tttgattcaa tcgtcttatt ttaccatagt gaaagagtgt tttcttcctg cttaagttgt
    25321 agttcaatat gtttttttac gttagtgcaa gtagagtaac ataagaatga acagaaaaat
    25381 gtaagttttt tcttaaatat gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa
    25441 ggagtacact tgtgaaaatc acgatcactt tacctatgaa ccatcctgaa acaacaattc
    25501 gcgacttgct tgaaaaggaa tggttagttc ctagaaaggt tcgacacttt ttgcgtatac
    25561 gtaaaaacgt ttggatcaat caacaacctg ctttgttcca tcatgaagta aagggcaatg
    25621 accagatcac attgttattc gaagaaacag attataatta tcaagaagtt caattaggag
    25681 atgcgaagca catccgtgtt ttatatgaag atgagcattt gattatagtg gataagcctg
    25741 caggaatgaa gactcatcca aatgaaccga cagaaaacag taccttactc aatcatttag
    25801 ctgcttatct cgacactaaa cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa
    25861 caagcggtgc cgttttattt gcaaaaaatc catttgtctt gccgatactt ggaagaatgt
    25921 tggaacaaaa aatgatctat cggagatatc aagccatcgt atggggagaa attgccaaag
    25981 atatgaccat caccaataaa attggacgag atcgccatga tcggagaaaa agggtcgtag
    26041 atgataaaaa tggtcagagc gccttgacac atgtcactgt caacaaagta ctttctgaac
    26101 aaacacaagt ctattgtgtt ttggaaaccg gacgtacaca ccagatcaga gttcatttag
    26161 ctcataaggg acatccgatc gttggagacc ctctctacca aaatagaaca gctgaacgac
    26221 tgatgctcca tgcattagaa ctgcacatga tccatccatt tactcaagaa aaaatagtcg
    26281 gtaaagcttt acctggatta tgggaataga aataaaaaag aacagagtct tatcagcaga
    26341 taatgataag actctgttct ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc
    26401 tgctaacagc aaactaccga taatcccact ttcattcggc agtccccaac gaacgatata
    26461 ttcacttact tgaggtgttt ccatataacc acccatcaat tcaacaaatt gctgacggat
    26521 tttttgaagg agatgatcct gattcattac cccaccgcct aaaataatct tttcaggtgc
    26581 taacgtcaac gtatagttga tcagtgcttg agcaatataa taagcctgaa tagcccaaac
    26641 aggatgatct tgcggcagat cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc
    26701 tgctgctaat ccttctaaac aatctttatg ataaggacaa gtaccttcat aatggtcatc
    26761 tggatggcgt ttcacccaaa tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc
    26821 ttcattgatc acgacacctg caccgattcc tgttcccacg gtaaggtaaa tacaactatt
    26881 ttttccttgt gctgctcctt gcttcaattc cccatatgca gcagcattga catccgttgt
    26941 ccaagcagca ggaatattgt agcgttcctt gattgtgcca agaaaatcga attgtgccca
    27001 cccttttttg ggagttgcca aaacgtatcc gtaattatct tttgttttgt cgataccaat
    27061 cgggccgaat gagccaatcc cgattccctt caattcataa cgatcaaaaa attcaaatac
    27121 ctgatccaat gtttcttctg gtgttgtggt agggatactt aattttttct taattgtcaa
    27181 attaagttag acagatcatc aatacttacg taactcaaaa atacttccaa aggtgttcga
    27241 taatccagtg attttctagg aatattattt cgtttagatg cgacagattg aataaaaaat
    27301 tcatctactg aattgaaatc catagatttc aataaaccat ctctacgtaa caatccgtta
    27361 gaattctcgt ttaggcctct ttgagacggc gttcctggat cagcgaaata aatggcaata
    27421 tcattgacat tactgatctg tttccaattt gaaaattcct ttccacaatc aaaagtgatg
    27481 gatttgaata ggtttttcgg tacagattca aaccattgat ttaatttttt ttcaatatca
    27541 atcgcttgtc taccacacgg tttcaatgtg atgataactt ttgataatcg ttcaactaag
    27601 gtaattacag cacttttatg tttcagacct acgatagtgt caccttcaag gtgaccaaac
    27661 tcatttgaga attggctata atccttttca cgttcatgaa tagagcggcg gaaagtttgt
    27721 tttccacgtc tttcttgatg tccattcggt ttacgcttgc ctttcatcgg taagtcagaa
    27781 gaatcaaata gccccttttt gaacatacga taaatggtac gagcagaaca actaatagga
    27841 aacgctgcac gaccaacaat cacatcgggt gtccatcctt gaacaacctt tctttgaata
    27901 tattctgatt gttcctcggg taaaacaagc ggacgtctac cacagtttga tttgtttttc
    27961 ttatactgtt gataatattc taaggctgat tttccttgct tgaagaacag atatacttta
    28021 tggatcgttt gtcttgaacg attcaagcaa tgggcagttt tagcaacaga ttgattcatt
    28081 ttgaaataag actctattat tacaagttca tccgttgtaa gatgggtata ggtcatttgt
    28141 actcactcct tataattctt tggtcggaac tattttgagt gtagcacaaa tgactttttt
    28201 agttgtctag cttaatttta caatcggcgt ttctaaaatt tctccaccat ctgaaaccgc
    28261 acacacaaat tttgttccgc ctgcttctat tccgccatac attcgttttc ctcctcgaat
    28321 ccgattgtcc gaatctcata aggttggatt attgggacaa ccgcttgctt cgttggttct
    28381 tcaagcagat tcaatatgac agtttttcca ttgtctttag ttatttccaa tttttctaaa
    28441 tgactagaca tattgaaacc tcttactact actttatcac taaacttgct gcgtttcaaa
    28501 gctgtaattg caaacgtttc actttttatc gttaaatatt gttgttttga aatcaatgtt
    28561 ccttcatgat gtttgatttg ttgagtggaa aaaggaatct gtgctgcgta tgcatgcaaa
    28621 taagtagaga atttttcttc tggtccatgt agttcaatgc tgtaattgaa ccgatgctca
    28681 cctagacact gtgcttctgg tgttggaaaa tatccccaat cgcctagttc tcccacactg
    28741 cgaagtagcg tcactgcgat ttgtccgtct tctgttactt catattcatt caaaccaaag
    28801 tttccgactg ttacaccgta ctcttcatca tgaatattga caaatgcttg ctgatgttgc
    28861 ggattcgtgg ggttttccca acttttagac acatggtttg ggcgcttcac cacttcaaaa
    28921 atactatctg cttcgtgatg ttccacatgt aactttgtag gaaacaagac acgtaagcga
    28981 tgatctttca tctgattgtc gagagtcgtt tcaaagtcga tttttttact atcttttctg
    29041 attgtcatga ttgtttttat ttgaaggatt cgtttttctt tggatcgttc tgcttttcga
    29101 taacgaaatt ccacaaccat ctgctgttct ttttctaaac gctcgtctgc agaaacaggg
    29161 atttccattt cctgtagaat acttattttt gctatttccg gtgtatctac taatactttt
    29221 acttcagagt tttctttgtt actggatagg attggctgat cacagaaagg ttgtttaaag
    29281 atatactcat ttcctatatc gccgatatct tcaaaaatat gcagattatt caactttcgt
    29341 ttgtttttgc gatcttccat tgtaatggta ccattttttt caatcgtcaa atgtagaggg
    29401 ccgttttcga tgattcgtcc actctgatga atcattgttt ccttttcttc tgctttcgct
    29461 tccccttcta caagcgcgaa tgtttcccaa gaaaatgctg gcatgttttc taatggtaaa
    29521 gtcactttga tcattcttgc catatatggc acacggaaac ggtctttcgg caaatcataa
    29581 ccaaattgga cagtttcttc agataaaata gctggtatag tggccccact ttttgtttct
    29641 acatgatact tacgtttagg caaattttct aactcttgat atagttgttc aggtattcct
    29701 tcagcgaatc tttttctttc taattcgatg gttatttctg cttcaccagt tttatccatt
    29761 cctgcagtat ttacaatgac aaatggaaag ctgtcttttg ggaatccggt cgtatcgatt
    29821 gctgcggtca gctgttctaa ggaatcttgt gcaagatact ttccaacttc atctgctttt
    29881 tcaaaacgag gtatcatttc ccgatggaca gaatcgactg aacaaccgca aatactgtcg
    29941 tgaggatgat tttgcattaa tgtcttccaa gcataatcta attgatcatg gggatagttt
    30001 ccactaactt cataagccat cgtcgcaagt ggttctgtaa tattttctag ctgtcgctgg
    30061 accttggtat tccattgttt taagtaaacc cgagcagatg ctgtattggc taaagtatac
    30121 cagccatccg tttcttgact tgtcaattct ccttctactg agcctaagtc ttctggcaca
    30181 tctttttgaa ctgcttccag atagtcagta aaattagaat gaataaactc gtaatctgga
    30241 aacagttcat ttgcgagatg gattgctttt gatatatctt tttgtaccgg ttgatgatca
    30301 acaccattca tcatcaataa gtggttcgta gaggcatatt gttctgcatc cgctaatttt
    30361 tgcttccaaa aggcaagagc tgcttctttt tctactggaa tctcattccc gttgctatac
    30421 caattggcaa atagcagacc aaagattgct gtttgatcag gtcctttcca ccacatctct
    30481 gagtattggg aagaatagtt ttctgcttcc aaaacttgat tatcaaagcc aattggtttt
    30541 acaccacggc caaacgctgc tgctgaaata ccagcttgtt tcattaattg aggcgtctgt
    30601 cccatatttc caaatgtatc gggaaaatag cctaacatga caggggttcc ccatttacgg
    30661 ctttcttcca tgccaatcag catatttctc acgttcgatt ccgagctgat aagaaaatca
    30721 tcttgcaaga tataaaatgg tccaattctc aattttcctt cattgattgc ttgttggatt
    30781 gcctgtcttt tttctggcct tacttgaaga tagtcatcta aaataatcgt ttgcccatcc
    30841 aaatgaaaac tgttgaagga cggatcaact tcaaatagct ctagtagatc gtctatcaat
    30901 tcaatcagtc gcatgtgatg ctgttcgtaa gccatgtacc attcacgatc ccagtggcta
    30961 tgagaaatga tatatacttt ctttttcatt tacgatacct cctacttttc tactctaata
    31021 tcaaagtaat ccatgactaa ttcgcaaaac atcatatttg cccaagaaaa ccattctctc
    31081 gtataatttt caggattgtt cacatcgaat ccttcgtgca taaggtgtgt tcctgcgtca
    31141 gttgctacaa gtaaattcaa tatccgctct ttttcagatt tgtctgacgt tgtcattcct
    31201 tccatagcta atgcaatcgg ccaaacgtat ttttctggcg tatgggagga tccaattcct
    31261 tttgcgtact ttccttcata gaaatatgga ttttctttac tcaacaatgt tcttcttgtt
    31321 tgcagataat cttcctcttg ttcagaacaa tatcccaagt aaggtgcggc gatcagattg
    31381 ggcacattac tgtcatccat gactgtggcg tttcccattc catctacttc atatgcataa
    31441 atcgtttgcc ctttttgatt aaccgttcgt ccgtgattct gaatcccctc ttggatctct
    31501 ttcttcaatt tctgtgtttg ctgtacaatt tctttatcat cgagtatcgt ttgaaaaatt
    31561 tcttccacat aacctaacac gaccaccgca aacatattgg atggtaccag atatccataa
    31621 cggcaagtat catcgctagg acggaaacca gaccatgtca ttcctgtaaa agctgttttt
    31681 gcacctcgcc cgtcattgat cagcgtatct tcttttcttg tcgtgtctcg tacaaatgta
    31741 taaggagagt gttcatgatc ttgctctgtt ttgaacacgt ccaagatttt tttgatacct
    31801 tcaataaaag attgattgaa ttgatcagtt tttcctgtat ttttgtaaag aagataggcc
    31861 aactgtattg ggtagcataa agaatcaatt tcatattttc tttcccagat ccaatcgttc
    31921 atttttgtat gatctgtttg gtggccagca ccgttagctt cttcgttgaa ggcattcgca
    31981 tatggatcga tattgatata aaaaaattgc tttttgacta acccgctgat catgtcagct
    32041 aattcctcat cttcttttgc aatcactaaa taaggacgaa cttgtgcagt agagtctctt
    32101 aaccacatcg ctggaatgtc tcccgtcaat aaaaaggtgg ttccgtcatt ttgctttttg
    32161 actgttgtca acagagtatt ggcaaatgct gctgtaaaat tctttgccca gtccgcatgg
    32221 atatccccac atttttgtgt gatatcttcc ataaatttct gcaccgattt tggtatatct
    32281 ttataagcca ctaataaaac cttctttcta tttgatatat ctttaaagat tatacttcat
    32341 acagccaatt atgtctacgc tttcaaaaaa tatcttttta tttacttttt catccattat
    32401 ggtattattt gaatatgata tatcattaag aggtgtttat atgaatcaac cattgtacca
    32461 gacaatattt aatgatttgc aaacggctat tttaactggt gaattacctg tagacagcca
    32521 actaccaact gaaaaagaac tttcacgtac ctataaagta agcagaatca cttccaaacg
    32581 tgcattgact gaattagagc aattaggact tatttatcgc gttcgcggga aaggcagttt
    32641 cgttaaatcc gcttctccgg aacagattct tgcttctcct acaaaaacac gacggattct
    32701 gtttcttctt ccatatgtcg ctgatttgtc tgttggtgat tttagcaagg gattgaaccc
    32761 tgtgatgcaa gataaacaga ttgatgtcat gatgacgacg cttgactttc tacaaaataa
    32821 aacagcaaaa gagctaatca atgaatttga cggattgatt tattatgctc ttgatacaga
    32881 acaacatctt gacttacttt ttgagctttc attgaaagaa ttccctgtcg tcatattaga
    32941 taaaaaaatc tatgagttac cttttcctac cgttctttcc gataatttcc aaggcggtgc
    33001 gcttgctgcc tcttatttga tcgaacaggg acattcaaaa atcgcttatt tgtttggtac
    33061 gcagactcat ccacaatccg ttcgacagcg ttaccttggt taccttgaag cattggacaa
    33121 agctggtttg actttccaca cttcactgga tgacaaaagc gcgacattag atgaattgct
    33181 ttcttatatc actaagtatg aagtaactgc ttttgtttgt gaaaacgatg ttgtcgctat
    33241 ccagaccatg aatctcttga gacagcatgg ttatcatatc cctgaagatt tttcaattat
    33301 cggatttgat gatatccaag cagcagctct tgttgacccg ccactgacaa ccatcgcaca
    33361 agattttgaa cagttaggta agcttgcggg agatgctttg gtcacttggt tagagacaaa
    33421 agatcgacca tccgatagta aatttccagt ttcattcatc aaaagacaat caacaaagga
    33481 gaaataaaat gaatatcggt caaatagata ctagacatgc aacagcgaat cagccaaatt
    33541 tctctaatgg gaactgccag ccttatactg gcgttccttt tgggatgaat tattttgcac
    33601 cgcaaactac ggatcaaaac ggcagctggt ggtttcatcc tgatgaccgc gtttttcaag
    33661 gctaccgtct tacccatcaa ccaagtcctt ggatgggtga tttcagtcat atgctgttta
    33721 cgccgatcaa tggaaaacta caggagaaca cccttttcca tgctcaaagt tcttaccgcc
    33781 cagaagaaag tatattttgc ccgactcatc tgtcgatctg tcaattacgt tatggcatct
    33841 gttctacatt gatacctagc atgtatggcg gaatcttatc tattgattac agcaaaaatg
    33901 acagcggttt acttttatcc tttccgggac gccatcagct ctttgttata gatccttaca
    33961 cagtggaagg aaaaatcaca cattttgctg gttcagaaga tcctgatttt tctttttatt
    34021 ttatattgag atttgaatat cctttaacaa ttgatgagtt agctgtttcc aacaaaaata
    34081 gcgactcgat cccgttgtat tttgaacaga caaaaaaaca aactatccga tttgggacat
    34141 cttttatcag ctcggaacaa gcagcttata acctggaact agaacaaaac caaagcaaag
    34201 agacttacct tgctcacagt caagaacaat ggcagtccta ttttgatcgt atacagctca
    34261 cacaccatga tcaacaacaa ttgcggacgt tttatcataa tttatatcgg gtgtttttat
    34321 ttccacaaac attttatgaa attgatccta gtgggcaaaa aatccactat gatacgacta
    34381 gtcgatctat aaaacctggt gttctctata caaataatgg gttttgggat acgtataaaa
    34441 cggtttatcc tctttattct ttgattgctg tagaaaaata cgaagaaatg ctggaaggtt
    34501 ttttgaacag ctatagagaa agtggctatt tgccaaaatg gctgtctcct gatgaacgtg
    34561 gacttatgcc tgggacattg atcgatgcgg tcattgcaga tgctgctgta aaagatatcc
    34621 gacgagactt gatgcctgaa tttctaacgg caatggaaaa aggtgctacg gttcaaagca
    34681 aacatccaaa ttatggacgc caaggaacag aagattactt gacatatggc tatgttccta
    34741 atcactacca cgaatcagtc aatcacacat tagactatgc ttatagcgat ttttgtatta
    34801 gccaagttgc caaggtacta gaagaagagg aaatgacgaa gaaatatcag cagcaatcat
    34861 tgaactatca aaatatcttt gactatgagt caggatttat gcgttcaaaa gataaagatg
    34921 gcaagttcag agaaccattt tcagctaatc gttggggaca agattatgca gaaggaagtg
    34981 cttggcaaag cagctttgct gtttatcacg atttttcagg actgatcgga aaatatggtg
    35041 gcagtgagag atataaagaa aaactcattg agctgtgcaa tcagccccca acgtttgacc
    35101 caggaggata tggttttgag atccacgaaa tgagtgagat ggccgcgata gaatttggac
    35161 aattagctat ttcaaaccag ccaagttttc actatcctta cttgttcagt tatataggac
    35221 aacctgaaat ggcacagcca ctgcttaaac agttgatgac tcagaccttc gacgattctc
    35281 caatgggtta ccctggagat gaggacaatg gaagtatggc cgcttggtat atcttcaata
    35341 gtctgggatt ttatcctgtc acgcctggaa ccggtgaata tgttatcggt atgccattaa
    35401 ttgacaaggc tgttttgctt ttgtcaaatg gaaaggaatt gacgattgaa gcaacgccta
    35461 atcggccgca gcaacaattt atccataaaa tcaaacggat ggacaaagaa tatacgaaat
    35521 tatattttac ccatcaagat ttgctgaaag gcggcacgat ctcttaccaa ttagggatca
    35581 tccctcaccc taagaaatat caagcaaaag atttgccttt ttccttatca aactataatt
    35641 aacaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca agcaaaaata
    35701 gatcctcata tttttcgttt ggttggtctt attaccgcag atgcaagctt ttgaacaccg
    35761 tttattgaga ggttgtgaaa tcagcgttct gacctgagta ttagagagac aaatggcaaa
    35821 atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg attgaacacc
    35881 gtttagtgga gaggtcgtga aaaagctgtt tagtttcgag tattaagaaa aattttgtga
    35941 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
    36001 tccgtttatc gagaggtcat gaaaaagctg ccttgcctca agtaataaga acagcccaaa
    36061 attagatttc tagacaattt tttataagtt atttcattac attggctttt tcttttatga
    36121 ttctccactg ttttctcggt actctttagg ggatctgccg acgattttct tgaataattt
    36181 agagaaatac gtggtgttgt tgtagccaat ctcacttgct atctcattga tgttttgatt
    36241 actgtgcaaa agcagttgtt tcgctttttc catacgtaaa tggttcaaat aagctgaaaa
    36301 actttttttc gtttcttttt tgaacagctg tcctagatac atgacattta aatgcaggtt
    36361 ttctgacact tctttgagtg taagtggttc ttgataatgc tggtgcagga ttacgattac
    36421 tttttccaca ttgtcactgt actttttctg tctctgatgc tttttgatga atgacagaag
    36481 tagctgctga agttcttgca ccgtttttgc ctgatggatt ttttcgattc cttgtaaata
    36541 ttcctcatct tccagatcaa tcaattcccg ttgaatctcc ataaacagta aaaaactgga
    36601 atatcgtata tcttccggca tcatggctgc taactgaaac tgttcaaaaa aatcagtgat
    36661 cattttttga gcgacttcca attgtttatt ttgcagcacg cgtctgaaac ttgaaaaatc
    36721 gatggtttga tcttttgttt ctgcttgtac tgcgtaaaaa acatgctcgt ctttatctcc
    36781 ataaaattga tgcaactgta gattgtcttt cgcttgttgg aatgtttcag ggattttgtc
    36841 caaagaatct gtctcttccc cgatactgat cagccattcc tgatcaggaa agttttcttg
    36901 aacaaaacaa cagaagccat caacttcatc tgcctccaac aacaacgtga tcaatacacg
    36961 gtctccgtaa tttcgttgat agtaaaagat ttgttgccgt tcttgcaacc atctattgat
    37021 aagagtatca tgagttcttg ccagttgaat caatactatc ctatgctgct tctcacttat
    37081 ttgtcctatt atttcttctt cacttatttc atccaattct tcattcaacc attgactgaa
    37141 taacagttct tgatagattt cttgttgatt tttttgttca ttttgctgat ctaaccgtgt
    37201 tttgattttc ttgaggcttt caaccaattc gaacttattc acgggtttca tcaaataatt
    37261 cacggcgcct aattgcatac ctcctttcaa ataatcgaat tcttggtatc ctgataaaat
    37321 cataaattca aaattatgac gttctttttg agccgcttca ataaattcca gtccatttat
    37381 ttctggcatt gtcacatccg ttactactaa atcaatctgt cgctgttcaa gtacagctag
    37441 tccttctttg gcattctctg ctgttgctac tatttgaaag cctaaacttt gccaatcaat
    37501 tatctttttt aacccattca aaatcatgta ttcatcatct accaataaca cttgatacac
    37561 gctcatccct ccttgaatgt aatagaaacg gtcagtccac ctgtctcatt attctcaagc
    37621 gacatataat aactgctgcc gaagctggca cgtaaacgtt cattcacgtt ttgcagaccg
    37681 atagaaccgt gaagttccac tttaggatga gacagttttt cttcaatctg ttttagccgt
    37741 ttttcagtca tcccttttcc attatctttg atgatgatcc ttacccgttt gccttcctgc
    37801 aagactttga cacttagcgc attatcgaat cgagtaaaat caataccgtg tttaaaataa
    37861 ttttcaacta atggctggat cacaaattta ggtacttcga tcttttccaa atcaggatcg
    37921 atcataaaat gataggctac tcgattagga taacgcattt gatacaaata gacatatttt
    37981 tcgcaaaagt ccagttcttc ttttaaagtg atcgttttct cttgattcgt attgtttcga
    38041 agcaaagcgg aaaacgcata cacaacatct gctaattctt cacttccttc gctgattgca
    38101 tacattcgga tatattctaa tgtattatat aaaaagtgtg gattgatctg tgcttgaagc
    38161 gcccgcatat gtgcgtcttg ctgtttgatt tctaatttgt agatgtcttc tacaaactga
    38221 ttgatgctat ccaacatttc gttgataccg attgaaagtt ccttcagttc atattccgtg
    38281 tttgttatgt cgatccgcgt ctctaaattc cctgctgctg tttgatccat ggcctgcatg
    38341 acgatagata cttgctgtga atatcgtttg aatgttttat aaagaaagat aagcagtata
    38401 ttgtctaaaa taatgatacc aatgatcagc ggggcaagat tttttcccgt ttcgattttt
    38461 acactttttt tatctagtac agctaatata cgataatcat cagacagttc ttggtgttga
    38521 acaaaattgt ttttttccag atcgtcaatc ggcaattgtg aatttttctt cagactctct
    38581 gtgatgatca tttcttgtct ttcaaacgtt tctttagcta ttttattatc atggaatgtg
    38641 tatagtcgat ttgtcgttcc agaaatcatg tataaagaga gtccattgaa tgacgttaga
    38701 ttttctaaaa tcatatctag ttctttcttt tgaaaaccaa taaaaatact tcctgactca
    38761 gctcctcctt gtgtgatagg caacttgata taaaaggcgt ctagtaattt tggtgtcccc
    38821 ttttttattt ccccaccttt gttttctcta gaagattcat aatactccgg tagttgattc
    38881 aacacgatat atatcgcact tagttcttca taatttgcgt agagatcttt gatttgattt
    38941 gggaaagaaa ctaattcatc ggtactgttt tgctgttcgt atacatacat cagatactga
    39001 tctatgggtt ttgttaagta ggtcgtcaca ttttcgatct tttcggaatt ccctacaagc
    39061 tgttttttga tgatcctact caaggttgtt ttatcattca gcattctgct tgtgctttga
    39121 acggcatcac ttgtcatgtt ttctgcctgt ttttgatttt gttcccaagt atggatacta
    39181 aaaataacgg tagcagtcat cgttacaccg atcatgataa ccgcgtattt ccgcaacaga
    39241 cgatttagta aggttccttt tgaaaataga atccgaaact tattcataac tttccaactg
    39301 tttatctaat tcttcacgcc aatgtgttgt catcgtacca ttccaaaccg tcaaaagacc
    39361 aatgacacca aacaggatca atcctttgaa ttgccaagtc acgcctaaaa ttataccgct
    39421 accaataatt atttttagaa aagacgaaaa gctggaaaaa accgaaataa aacttagctt
    39481 tagcagatta ggaacagaga tcgtatattc actatctaaa atcatactgt attgataagt
    39541 agcaaatagg cagagcaagg caaaaagtaa aataaaatca atagctaaaa aaatgagccc
    39601 tttgatttga acagataaat ataaattgta actcaataaa gcagtaccag ataaaaataa
    39661 actaaatagc agattgcctc ttaaaaaatt tcttttatat atattccagc ctctttttat
    39721 tgtgatttct ttatactcga atccgtacaa ataaaatagt tcattgactg ttttccatgc
    39781 cgcacctaat ccaaagatga tcccgccggc aaatgtatat atccagaaat acaaggttag
    39841 ttttataatg acccagatcc ggataaataa ctcttccaaa accttcccta acatatcttc
    39901 tcctccaaac attaagttta tactttctca ttatacaaaa aaaggttggg caatcagcac
    39961 cttgacctga ttattagaga aacaaacgga aaaatagttc ctcatatttt gtcgttttgt
    40021 gagcgaatac cgagggttag ctgattgaac accgtttacc aagagtttgt gaaatagccg
    40081 cttagtttcg agtagtaaga aaaattttgt gaatatggct ttccacattt ttacatatat
    40141 ttagcttaat accgagaaac tggcttttga acaccgttta ttcggaacaa gaggttgtga
    40201 cacgtctttt gtcacaaccc ctttgtcttt attcattatt attcttgtcg gaactcatca
    40261 tattgttttt gcatttcttt caatacctta tcatagccag ccttgtccaa agcgtctttc
    40321 aatttcggta atgtttcatc tggatcaaca gtccctgtat tcaatccatc taaatattga
    40381 ttcatgacat tagaaatatt acttaattct gttttgacac tgtcagtgtt gaagctgaat
    40441 cctaaaatcg gtgaagtttc tgcatcagca atcgattgat cacgtttggc aatcatgtca
    40501 tccgtaatag actcttgtgt atatagaatc ttgttgtttc ctgtattcca agcagacata
    40561 tgtgtatttg gctgataacc atctagcaat ttgattttat catctgaacc atcgactttt
    40621 tcccatgctt ctccctcgat cccccaaaca agcccattca ataattctgg atcactgtta
    40681 agcagcccta gaagttctac tgatttttct ttattttttg aagtatttga tacgacaaaa
    40741 tttgccattt gtgcttgagc tgtagatttt agtggaacag taattgcttt tgagacaagt
    40801 tcttgttttg ctgcatttgt caaaattgta tcgccataat catacggtcc ttgtgtttct
    40861 ccacgcatta gccaagtatt tccttctagt ggataatcct tattgctcgt tgcagcgtca
    40921 cttggaatga gtccttgttt gtaccagcta tgcattgtac gtaacagtcc gatatacgtt
    40981 tcattatcgt attggttgat gattttcgaa tcatctccat ccagatcaat agcaaatggt
    41041 aacgtattcc ctaaaggata atcaaagttc ccttggactt tgaagccttg tccaatggca
    41101 aaagctgccg tgtttggttc tttttcatgg aattccttca agactttttc tgcatcagcg
    41161 taagattgga ttccatcaat tgaaagatcg tatttatcta gtaaagcttt gttaaaagta
    41221 agtacttgtt gagcaaagac atttccattg actgggaaag catataattt tccattgacc
    41281 agatttcctt taatatatgc ttcatctaaa tcattataag ctttttcagc gtacttaggc
    41341 gctaattcag tcaaatcagc aaaggctcct ttttgggcat tggaaacata gttgttcgca
    41401 aaggcgatat cataattttc cccggaagaa atgatgacgt tcattttctt ttcgtaatcg
    41461 ccccagccaa tgtaattgat attgactttt acaccgatct ctttttcgat tcgtttgttt
    41521 gctacttcca tcaattgatc gaaattatct ggtttatctc cgatttgata catctgcaaa
    41581 gtgtccgaat tgctagcttc tgcctggtct ttagaaccag aacctccgca tgctgctaat
    41641 gttcccaaca acaacgctga aacacctgta aaggctagtt tcttccataa cttcattttt
    41701 cttcctccta attttatgat ttatattttt gtttctttat tcttttactc cgccgattgt
    41761 tagacctttg acaaaatgct tctggaaaaa tggatagctg atcgcaatag gcaacgtcga
    41821 gatgactacg atcgccattc tggcagattc tcctggaact gaggctaatc cgcctgtcaa
    41881 ttggcttcct gctcctgaat tttgtgtcag atattgaata ttactttgga ttttcatcaa
    41941 aagatactgt agtggaacta gcttatcatc ttggatgtat agtaaagcat tgaaccagtc
    42001 attccagtaa cccaatgcgg caaataaact gattgtcgca atacctggaa cagctaacgg
    42061 taaaacgatc tgaacaaata ttcgcagttc gctagctcca tcgatccgtg ccgattcaat
    42121 aatcgaatca gagaccgaac gtttaaagaa tgtgcgcatc acaataatat tgaagggact
    42181 gacagccatt ggcaagatca atgcccaaat cgtatctttt aactgaagaa gatttgtcat
    42241 aacaagataa ttggccacca tccctggtga gaacaacatc gtaatcaaac aaaaaacggt
    42301 aaaaaaacga cggaatggaa agtttgaccg tgaaatcgca taagcatata aagaagtagc
    42361 cgtgctattg atcagtgtac ctaaaacagt aacaagtaca gtgacgaaaa gcgcttggaa
    42421 aattttatcc tgcatctggt cgaacaagta cgtataacca aatatactga acttttctgg
    42481 ccaaaaactg tacccgtgag tcgacaatat ggactcatcg gtaaaagaga tggcgataac
    42541 aaatataaat ggcaaaatac aggaaatagc aaagagtgca atcattatat tagagaaaaa
    42601 attagccgtc ttattgaaag agcggacttg gactctctcc actttctttt ttttcatttt
    42661 tttcctcctt agaataatgc agaatcgcta tcaaaacgtc gggcgatcgt atttgtgatc
    42721 atcaacaaga caaagccgac aactgattgg taaagtcctg cagcagccgt cataccaaaa
    42781 tctcctgtgg atgtcaggcc attgtagata tacgtatcca aaacttgagt cacatcatat
    42841 aatgcaccgg aattcctagg aatgttataa aacaggccaa aatcagctcg gaaaatgttt
    42901 cctacggcta aaatcgttaa aatcgtcatt agtggaataa gttgtggtat cgtcacatgt
    42961 ttgatctgct gccatttgct agctccatca accatagcag cttcataata agtcggatcg
    43021 attcccataa cagatgcaaa gtaaataatg ctgttataac caatcccttt ccatgtacca
    43081 atgaacacca agataaacgg ccaatatttc ggttcattat accaattgac agcttctcct
    43141 ccatgtccag tgatccactg attgaatatt cctttgtcag gacttaaaaa agcgtaaaca
    43201 aagtaactga taatgaccca agatagaaaa taaggtagta aggacatcgt ttgataaact
    43261 ttaacgagtc gtttatttct caactcgctc ataatgattg caaatacgac tgagatcagc
    43321 agattcaaaa ggataaatgc tacattatat aaaattgtat ttttagtgat gagaaatgct
    43381 tgatccgaag aaaataaaaa tttgaaattt tcccaaccca cccaaggact ttccctcaag
    43441 ctagccaaga acccatctgc tgaaatatga aaatttttga atgctacgac attcgccatt
    43501 acaggtatgt aaaagaaaaa aatcatccaa agcatacccg gtacagccat caggacaagt
    43561 gctttataac gccatagatt cataaaaaaa tgattgaacc cctttcgttt tttcatctaa
    43621 aaaccccttt cgattcattc gcaacgtaat tatagcgctt tcaataatga gaataaataa
    43681 acaaagtata gggaaaatga aacaaagtat aggagaggtt gtgaaatcag cgttttggcc
    43741 tgagtattag agaaaaaaac gggaaaatag ttcctcatat tttgccgttt tttgagctaa
    43801 taccgagggt cagctgattg aacaccgttt atcagaggtt gtgaattcag cgttttggcc
    43861 tgagtattag ggagatcgtg tccttactat ctaactctga gtcacaagga acaatgggga
    43921 ctgtaacaaa agtcttgtca cagtccctta ttccgaacaa acggtgttcc agaagtagct
    43981 tctacggaaa taagccgaaa tttcacaaaa aagccatccg aaagtcagac ggacaagtct
    44041 gcttttcaga tggcgctaca actactctgc ttattctttt agctatccat tcaattattt
    44101 ttctctcact tgttctagct cttctttggt catgggtcga tattgccctt tattcaaatc
    44161 tttaggcagc caaaattttc ccatacggat tctttttaaa tataagactt cttttcctac
    44221 tgctttgacc attcgcttaa cttggtgaaa ctttccttca tgcaaaatca gacggataat
    44281 tgaagtttct gtctcttgat ctacttgatc aatttggagt tttgccggca atgtctgctc
    44341 atcgccatca ataatcagtc cttctgcaaa taaacggata tcttcttctg tcattatacc
    44401 gctaacctca gctagatatt ctttctccac atgtttttta ggtgatagta actgatgggc
    44461 aaacaccccg tcattcgtta aaatcaagag gccctctgtg tctttatcta atcggccgac
    44521 tggaaaaaga tcttcccgat aatcctggtc ttccaataaa tcgattacgg tttgatcatg
    44581 attatcctga gtagcagaaa tgactcctgc aggtttatgc aaaatgtagt aaaaatcttt
    44641 ttgatacgta atgatttctc ctaaataagt aaccgcatct tcgaattcat ttacttgcgc
    44701 tttatcgctt ttcaccactt gtccattgac ttcgatcgtt ccttttttga ttaatccttt
    44761 gacttctttg cggctgccaa gtcccatatc tgctaaaaac ttatctaaac gcatgcgttt
    44821 cccccatttc aaaagagcat caatctagct cttctgattc acttatttta tttttaattt
    44881 atgtcgtaaa cgcgctattc ctggtcctag tagtctttct gccaagcgga ttttcaaagc
    44941 taaataacca tacactaaag cccctatacc tgctactaca agaatcaaca atagagactg
    45001 gaatttgcta tcttcgttta aaaaagcacc aaaaatcatc tttgtgatcc acgaagccaa
    45061 aagcataatc aaggtaagaa taaagatcag caatgtcctg cgccagacaa aacttggttt
    45121 aaagcgggct acttcatgga ttctctttaa gatcaaggta caagacacag caaatccgat
    45181 cattgtcgcc aataaagggc cgtatacttc aaataaacga atggctgggt attgaagaag
    45241 gagtttcact agaaacccaa taaccaaata attaattgcc gccttgtttt caaacattcc
    45301 ttgcagcatg ttggaaacta gcatatatag tcctaggaag agtcctacaa aacttgcttg
    45361 gatcagtaca ttacttccta gagaatccgg tgtataaaat aatgtattta gcggataggc
    45421 aagtaacata acaccaaatg tcgccggaaa cataaaaaat gaaaacaact gtaaatttcc
    45481 gcttgtcaat tttgccagac ctactcgatc tttcaacgta acagcttctg taatcaacgg
    45541 taacccagta gacgcgatcg atgttgccaa tgcaataact accatcgtca acttatctgg
    45601 attggcacta aaaatcgaaa aaagatccag taactgtgca ttggaatact cggtggatgc
    45661 agacatgatc ttcataaaag tgaactgatc gactaatttg aagatcgtta cccctgaacc
    45721 cacaataata aacggaatcg cttctttaac ggtatctaac aacaattcct tcgttgcaat
    45781 atttattttg ttttcgctgt agtgaataaa cgccgatgta tacgcttgat gtttcttcaa
    45841 aaaatacaaa aggacaagaa tacttgctag cataccgata aatgccgcga aagttgattg
    45901 cgtgaccgcc gtcacatagt caccatccat gactttcatg atgatgaatg tagccaatag
    45961 catataaaaa atacgtgcca cctgttctac aatctgcgac agcgcataag gcatcatttc
    46021 ttgattgcct tgcaaatacc ctcgtatcac actcatacaa ggaaaaacca atacagcgat
    46081 actcaaagaa cgcatcgttg gaatcagttc ttctccgcca ccagaagcat gagctagcca
    46141 aggagaggcg atgtacatga acaaagcaaa caacaggcct aacccgccca tcatctgtaa
    46201 tgcacggata aataatcgtc gtgaagtgcc atattcattc aatgaattat agcgtgcagt
    46261 ttgttttgca atcgcagcag gtattcctgc tgtagagatc agtagaaaca atgcatatat
    46321 attgtatccc atattgaata atccattggc agccttagca ttttcgccca tccaagcata
    46381 ccagggaatg atatagacag cgcctagcaa tcttgaaatg atattactgg cggtcatcca
    46441 agcagagcct cgtgccattt tttcttgcgg tgttaattga tctaatgctg gttttctttg
    46501 attatccatc ggtcagattc caactttcta caaaacatta ctatcatttt aattggtaaa
    46561 acagaaactg gcaatcgatt ttatataact tttataagaa caattgtttt tttgaattcg
    46621 gtttcgtttt tttggtaaag taaagaaata tacagccttt tatggctaac atttatagga
    46681 gatatcaatg acacaagaaa taaaaaaact acccgttata tttttaggtc ttttctttat
    46741 cacagttgga ttgaattggt tcttgctccc tcacgatata gcttccgccg gagtgggcgc
    46801 catcggacat ttagttgaaa cggccttttc tatccaacgc tcagttactg tttggagcat
    46861 caatcttatg atgctgcttc tagccgctgt cctattagga aaatcagtat tttttaagac
    46921 tgtagcggga agtctgcttt ttcctgttgt tttagagatt gttccaaaag tggaaatgat
    46981 ctcctcacat ttcttttcac tgatttcagg aagtcttttg ttcagtcttg gggtttacac
    47041 attgtacaca gtcggggcct caaatggcgg tataacgata ccgccgatta ttttccacaa
    47101 attttttcgg cttccaatgc cgcaaggact tttacttacc aatagtctta tcgtcttttt
    47161 gaattattat gtttttggat tgttggagac actattcgtt ctcctatcta ttacagtcag
    47221 ttcattgttt atgaaaatac tcatccgctt gtcccctgtg tcaagaaaat ttgaataaag
    47281 ctggattttg tcatagcgga aacaaacgat atattaaaaa gagtgtgcta taataaacca
    47341 agaaattgaa taaagcgagg gatcaatcaa tgagcttctc tttagaagac atccgtcatt
    47401 tactattaaa agaaaatcta ttaaaagaat ttgtttcaac aagaggctgg catcttgatg
    47461 taccagaaaa agctgaattt cttcagttaa gttatgattc aagaaaagct gatgcttcta
    47521 ctttgttttt ctgtaaagga atgaatttca aagaagaata tttggattca gcaatcgaac
    47581 agggtattca atattatgta tcagaacaac catacgaaaa ttcagcagta ggaatcatcg
    47641 taacggatat ccgtaaagca atggcgcttc ttgctatggc tttttacgac tatccgcaaa
    47701 ataaattgaa agtgatcgga ttcactggaa caaaaggtaa aacaactgct gcgtacttta
    47761 caaaagcaat cttggatcac acaacaaata aaaaaacagc attgctttct actatgaata
    47821 caacgttaga tggcaaaaca tatttcaaat cccatttgac aacgcctgag tcattagatt
    47881 tatatcgtat gatgtcagaa gcagttgaaa atggtatgac tcatctagtg atggaagttt
    47941 cttcacaagc ttacaaaaca caacgcgtat acggcttgac tttagatgtc ggaatcttct
    48001 tgaatatctc gcctgaccat atcagtccga tcgaacatcc gacatttgat gactatttct
    48061 attgcaagcg acaattgatc ttgaattcaa aaacagttgt tttaaatcat gaaagtgact
    48121 attttgattt acttagggaa acagccgatc tattccagat tccaacgatc acttatggtc
    48181 gttcggaaga aagcaattat caagtgatcc gttcagataa aggtactcac ggttttactt
    48241 tatcttctca tgaagatcgc ttagctatct gcgatactgc ttatgatatt ttgctggctg
    48301 gaggattcaa ccaagaaaat gcagccagtg cgatcattgc cgcagcacta acaggtgcct
    48361 ccagtcaaga tgcacaagac ggattaaaag aagctcgtgt accaggccga atggatcaat
    48421 tgatccaatc caatggagcg catgtctatg tggactatgc acataattat ctaagcttga
    48481 aaacattgct gaaatttgcc aaaaatgaac atccagatgg ccgtgtaatt gttgtattag
    48541 gcagcccagg aaacaaagca atctcccgtc gacatgattt tggaaaagtg ctatcagaaa
    48601 cagctgacat cgcctttttg acggcagatg acccagcatt tgaagatccg aaaaaaatcg
    48661 cggaagaaat caatgaagca attactaatc cagatttgat cgttcattat gaaatggatc
    48721 gtcctgaagc gattcgacgc gctttagcag aaagcgctcc gcaagattca gtcgttattg
    48781 cgggaaaagg cgtggatccc tatcaaaaaa tcaatggtgt agatgaacct tatgaagggg
    48841 attatgcaat cgtgaagcgg ttgattgaag aataatggta tttatatact agtggtttcc
    48901 catttctaag aaaaaaaggg actgtgattt tttgtcacag ttcctttttt cttagaaatc
    48961 attttaactt ttcactagct aattctgctg ccttttctct ttcttcattt aataatttat
    49021 tatcatagaa tttgataaat gggaaccaga taatgaatgc gatgattgca caaatgactg
    49081 ctaacacagc agctttccaa tcaccaccac taccaacaaa tgctccgatc ccgactggtg
    49141 aaggccacgg catctgtgca agcatcggtt tgacgatttc cagccgaatg gcaaaataag
    49201 cgagtgtagc agaagccatc ggagctaaga aaaacggaac cgctaaatag ggattgtaga
    49261 cgattggcat cccgaaaatg attggttcat tgatattgaa taatcctggt acaagtgagg
    49321 cttttcctaa cactttcagc tgatcagatt tcgcgaggaa tgcaataaag atgatcaatc
    49381 ctaaagtcgc acctgatcca ccaacggtta cataagaatt gttgaattct cctgccaacg
    49441 gataatttgc gccttgtgcg ttagctgcca tattagctaa tacgatcggg gtcaagaaag
    49501 aagtgatgat attcgctcca tggatcccta cgatccacaa tgcatggatc aagaaataaa
    49561 tcaccatgac accaagccat gtattcgtca actgcgtaac aaacccaaat gggatagcaa
    49621 tcattttgaa gatatccgtt cctagtgcaa ctaataatcc attgatgatc atcaccacaa
    49681 acgcaatgac aaatgttggg atcagagcag tgaaagaacg agaaactcct ggcggaacaa
    49741 catctggcat ttttatgacc caatttctct tgacacacat acaatacaat tcgaccgcaa
    49801 taatcgacat aataatagct gtaaagattc ctgaagttcc taaacgttcg acaaaagagc
    49861 ccatcctaat accatttact acagtttctg tctcattgat agaagcaatc aagctgattt
    49921 taccatcagt catgatcaat tcagggatac acataaagaa tgcaaaaaca gatagtaatg
    49981 cccccgtgag cggattaaca tttaattttt gttcatctcg ttcaattgac gttaattcat
    50041 aagcaaacac aatggcaaag taaatagata aaatccccat cgtcattgta ttgacgatca
    50101 tgtacaagtc actaactttg aaaaaagttg cattaaaaaa tccttctaaa aatgggaacg
    50161 gtaatggcaa aatatttaaa accaaaaaca tcgagcctac aatcgtgaaa ggaatagaag
    50221 ccatccccgc tgccatgaca gcccgaacga ttttaagttg cgcaattttc cccataggtc
    50281 ccatcaggta tttctgcaaa aaatcaaaca ttagttattc tcctttccta aatataacgc
    50341 tttttcatat tgtttaatac ggatatactg ttggtcttgg ttgagtgaaa ttttctttaa
    50401 acgatactgt ataaatgcac tggcaaaaat accgcttaca acaagaatga aaacaaataa
    50461 ctgtcctcca ttgttatttg ccataaaagg atacaagaat gtaaatcctg aggtaaacaa
    50521 cactggaatc actaataaat taaagacact ctggaacaaa aaataccact ttgtccatgg
    50581 aacgttcgtt tggtgttttc gataaagctt tacttgttct cctactgcag gcaaaatagc
    50641 taaaatcaga atcatcggta aactacttcc taaactttta gctgatagaa gcagaatcat
    50701 ccattgcatg ttcatgaaaa agaaaaaggc agtcaggtaa cgtacgatta gataacgatt
    50761 gaaatacatg tttttcaaac taagttgctt tttttctaat tcgattttcc tttttactgt
    50821 atcctgcaca atgatccttc ctttctattt atttttttga tacaaataat tcatttcctg
    50881 tgcaacttcc aataatgtca tcgtcgtcat cagatgatct tgtgcatgga ccatgataat
    50941 ttccatttct atttttgtcc cgctggcata ttctttcaga agttgagttt gtgactgatg
    51001 cgcatgaaga atctcttcat ttgcttgtcg caacttctct tctacttctt caaacatctc
    51061 tttacgcatc gagcgaaatg cttcatgtat cagtgtccgt gcatttccgc tatggaaaat
    51121 gatatcaaaa gaagtcactt gtagtgtttc actgtttatt ttttcagcca tctgttttcc
    51181 tccttattcg atcagtttga taaatttact gctgaaattc tcaaaaatag gttcagctaa
    51241 gatttcctgc tgtactttta gctggtcaac taacctaacg attgctttag tcacgaccgt
    51301 aattccttca ttttcgacat aagaaggcga aactaaaaaa acaaaatgaa tttcttgata
    51361 ttgatcccac tgcatcccag aaggaatcaa tgcaacggct acctttgttt cgattccaac
    51421 tggaacagcg ggatgtggga ctgccactgt atcactgaat atgatttgtc ccatattttc
    51481 acgttgctgg atctgctttc gcatttctct tatatatcct tcttcttcat ttatgctcag
    51541 cttttgcagc aaatcatgaa gaatttcttc ctttttcggt tcgtacgtat aagtcttgaa
    51601 aaatttttca gacaactgtt cggtgtaaat ctgctttttt tgctgtagag aaaatgctgg
    51661 tatcggtcta tctgaggaat gatagggtat agattctcca actacctttc gtattttacg
    51721 aacatcatct tcattcaaaa aaatactgac atgtaaaact ggaattttga aaaacattgt
    51781 cgaaagatca atagaagaaa taatcaggtc gattcctttt aaagttgttt cattgatctc
    51841 ataatagcct tttacactta ccactgtaat atttttaccg aattcactga ctacgcgatt
    51901 ctttaatagt tgggcactgc cgtaacccgt tgcacagata atcaatgcat gtagtttatg
    51961 cgcagctctt tctttttcca atgccgccat gagatgcaaa gccaaatagg cccattcatc
    52021 ttcattgatg gtatagtttt tcaaagccgg catgttgctg aaatgccttt ttactgcgtc
    52081 aaagactttg ggattttctt tctttatctc ctcagtcaat gggttctcaa tgcgaatacc
    52141 tcggctcaat cgaatcatca tcggtgacaa atgatcgagt agtccattga aaagctggag
    52201 atcctctact aatgaaaaat gaaattcctg agacaatctt ttcagaactt tcgctaattc
    52261 ctttgatagt tcctgatcat tttgattttc tttgtgattc gacttagcca tgagatgtaa
    52321 tgtcaaataa gcaatttctt cttcgggaaa cgatacacct gcgattacct caatacgttg
    52381 aacgatccgc tgcgccactt gatactctac tcgcttagaa acttcttgtt cgatgcctaa
    52441 ttccttgatt tcaaaccctt ctcggagccg tttgatcccc aaagccaagt gtaaagtcag
    52501 attctgtatg ataaaatccg aaacttttaa cttagcttct cttgtttcat cgagaatgat
    52561 aatgactagc tcttcaaaac tgatgtctgg gaaaaaacgg ctttgcccta aatattcttt
    52621 caatgaattt ccataatggt ttccaaaaaa agtatccatg ataaaatgcc gtcgatttct
    52681 ttcttcccct tcgatccatg tcccttgacc atgcttagaa acgattttta gtgaatatgg
    52741 aatcaatcgc tccttgattt cttgcatgtc ctttgtcaaa ctggaacgac tgatataaag
    52801 ctcttctgcc aaatcatcca taaaaagaat ggcatcttcc aaaagcaatt ggttcaatat
    52861 atacttttgt cgatcttcgg actcataaaa tccttgcggt tctctagcat atccaggtac
    52921 tatttcttgc cgactcaaaa acaagtcaaa tgtcagtctt tgtaaaatat gcagttggta
    52981 gccataacct ggtttagcaa tgatcttacc tccgtttttt tctactaact ctttgatccg
    53041 ctgcaagtaa ttacggattg tccgatcaga taataacaat tcagaagcta aacgttgact
    53101 ggtgacatac tccttcttgt gatcaatcaa atatctcaac agcaattttt ccttctgttt
    53161 cattatcatc cactcctatt ccatcatatg ttctcttcga tcacctctgc taacttctgg
    53221 atgcctgtag ggattgggac atacgcttgg aaagggatac taacaacagg ctttccaaca
    53281 gattctccta attttttgaa tttatccaaa aacatcgtcg tttgcgggct gatcagaaat
    53341 aaatcaaaat cactattctt aatcatatta tcgccttctg tagctgaaac tgcatctacg
    53401 agtacctctt ctcttttttg cttaaagaaa tccgttgtct ttgaggccat catcgaggat
    53461 gacattcctg ccgcacaaat aatcaatgct tttttcataa atcaatcact ccttttattt
    53521 ttataaacca agcatatctt gtaagcgttt tctattcaaa atatatattt ccgtagcatt
    53581 gcggaaaaaa tgtgtatcat atcttgaata ataatttctg gcaccgtgat atactagtga
    53641 tagaattttt tcaggagggg atttagtgag tatcacagtg aatattttgt atacaagtac
    53701 aaatggacaa gccagaaaat ttgcagaaga aatggtcgaa agaaacattg tcaaagcgat
    53761 tcgtgcagaa gaaggaaacg agaaatatga ctattacttt ccactagaag atccagaatc
    53821 tcttttactg atcgaccgtt ggaaagatga agaagcaatc gaaaagcatc acaaatccga
    53881 aatgatggct caaataaacg aactccgtaa aaagtataaa ttaaaaatga aagtcgaacg
    53941 tttcactgat ttaccgtaaa aagatacaca tggaatgata gcaatttatc atccatgtgt
    54001 atcttcactt tttccttatt ttttattcga tctcatctaa tgattcaaac cgtgtttttc
    54061 tcttattttt cggataagta tttcagtcac cccaatatac atgattccta aacatacccc
    54121 aaggatattc tcaaaaatgc ggatcgttac tgctcctgga accccgaata aactggcagt
    54181 gattgccaaa gcaccaaaag aattaaaaac tgttttatat ctataggtag aacaaacacc
    54241 taaagctaga ccacctaata taccgaccca gttaaaagga ataaattggc tgataccaat
    54301 aaataaaaca catccaatca atgagccaat gattcggtct actgctcgtt ctttgaaacc
    54361 aatcgacatc aatccatagc tagatacaat agaagaaaat gcaaaccctg cccacatcaa
    54421 tcgctgaaaa gggacatact ctcctgcaac gatcaaaaga ctaatgccaa atgcatagat
    54481 gagcatccaa attttttcct gcgaatacat gcctttcaaa aaaatctctt taaacaagct
    54541 tttgtctttg ttcttttctc gatgttttct gtaaaggaga actgaaaacc aacaaaaaaa
    54601 caaaaataat aagctacttg tttgtgtaaa gaaattcttg ttcaacgaat ctttaggtaa
    54661 cgaataaaca ataaagagat atgaaaagcc gtacaaagaa gcattcccca ttttgggatc
    54721 actagcggtt gccattaata tgctgcttag taaaataaaa tgaatcaaca tgcccataac
    54781 tgaccatttg atcagctgga catacggtgc tactaatagt gagaacatga cgattgctaa
    54841 acctatgatc gaatgggaaa tcttataacc aaagtcaaca aaacgaatac tcatcaacat
    54901 acagaataaa accacagcgt atggtttatg cttcgattca aataatgttg taattataat
    54961 gataaaacaa atggcaaaca atacgatgaa taatgatcgt aaaaacaatg aacaaatata
    55021 catgcacttt tcttttttag aagctgcttc tcgtattttt tgttttaaaa taaacggatc
    55081 tagttgtaga gactgataaa aatccacctg ttgtcgtcct ttctttttac ttttattttt
    55141 tacagtcatc tatgacaggt tgtaaatact atggaacatc atatggcgtt gtcattttct
    55201 tgtcaactat ttttcctatt catgttttta taaaaaaaca tatcgttttc ttgtatgatt
    55261 aaatcaccac aaaataaaca actgagagaa cgatatgctt tatgaatggt tctatcaaaa
    55321 aaattctgag atcaatagac aaaaatttga tgattacaga ggtctcttac gaaaccttac
    55381 agaagaaaaa aacattgatc gttgctgcag ttctcttccc tgctccatgt gtctgtagaa
    55441 attgtggttc tgctgtggta gatggaaata ggaaattaat ggtggtgaaa aacaagaaga
    55501 aggaaaccat tgtccacttt gaataatata atcatatgcc aatggttata cgcctaaaaa
    55561 agcagcgcta tacctgtaaa aactgtcgaa gccattggaa cgctcaaagt tattttatcc
    55621 gtccaaggca ctcgatttca aatcatgtca gacataaaat cacttcttta ctgactgaaa
    55681 aagtgtcgtt attttttatt tcgaaaagtt gttaggtatc tttgacaacc gttattcgca
    55741 cattgaaaga gttgagaagc tatttaccaa agtcttccaa aagaattctg tcttgagtgt
    55801 tgagggttgt gaatttcgtt ctcacgtttc cgttgaggat aaactgagtt ttatttccgc
    55861 ggatggcgag acagaaaaat taatcgagaa aatttaccta cacaaaaaat tcctcgatta
    55921 acaaactatt tccttagctg cactaatcct aaagaaatag aattcttagt tacagacatg
    55981 aacgccacct actttcagct caccaaacat gttctaccaa atgcgaaatt ggtgattgat
    56041 cgattacttg gtttttccac gcctttcaag gaggcgtaac ctttattcac gagttggttg
    56101 aagctttccg agacaaaaga cccgacttgt ttttctcctt attgacaaag agccattaaa
    56161 actagagaaa aaattacaac tgatcgctga ttatttgtag aaagaaaatc aaagtattac
    56221 ccaataaact tgagtcatta ggcaaaaaac actgcgaaac tcaagaatga ttttcgcagt
    56281 gtttttgtat attttttatt aactagtatt actacaaagg tttatttttc acttctcaac
    56341 gcagcacaaa attgtcttta cttatttact ggttgggttc aaccttctct actccgatac
    56401 taatatttgt ttgtataccc cttctaaaat cggtagtttc cattcaattg atgggtacta
    56461 gttgatttta aaaactatgc ttattcaatt ttttatttta gctcatatat ttcgtttctt
    56521 tctaaatttt atactgatga aaacaagtag cacacccaat attgacaata taaactggct
    56581 aatgatggtt tctccagttt ttggaagatg cttatttcca ctgcttgttg gattgtttcc
    56641 tcttatatct gagctatcag caacttttct attctctttt gttaaaaaga tcgccacttc
    56701 ttgttctttt tcgatcttga aagttactgg tgtcgcatcc aattcatacc ccgttggcgc
    56761 ttgagtttct accagctgat atgctcctgg tgctaagcca tctatcgcca gttttccatc
    56821 tgctcctgtg gttagacctg actgcagaac ggttccattg gcatcttgta actcaaaaac
    56881 agctccttgc aggacttctc cgctttggtc atcgatcttt gtcaacacga caccgcctgg
    56941 tgtaagtcga ttttctttcg tcaattctac caccgctgtc tgacttcttt cgatctcaaa
    57001 ctcgattggt gtcgcatcca attcataccc cgttggcgct tgggtttcta ccagctgata
    57061 cactcctggt gctaagccgt caatcgccag ttttccatct gctcctgtgg ttagacctga
    57121 ctgcagaacg gttccattgg catcttgtaa ctcaaaaaca gctccttgca ggacttcccc
    57181 gctttggtca tcgatctttg tcaacacgac accgcctggt gtaagtcggt ttgctttcgt
    57241 caattctacc accgctgtct gacttctttc gatctcaaac tcgatcggtg tcgcatctaa
    57301 ttcatacccc gttggcgctt gagtttctac cagctgatat gctcctggtg ctaaaccgtc
    57361 aattgccagt tttccatctt
//
LOCUS       DAZULG010000004        56510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000004 DAZULG010000000
VERSION     DAZULG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.004"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..757)
                     /locus_tag="ACG9CY_000171"
     CDS             complement(254..757)
                     /locus_tag="ACG9CY_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4936718.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1116..2141
                     /locus_tag="ACG9CY_000172"
     CDS             1116..2141
                     /locus_tag="ACG9CY_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4936719.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2464..3342
                     /locus_tag="ACG9CY_000173"
     CDS             2464..3342
                     /locus_tag="ACG9CY_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS4936720.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3818..3934
                     /locus_tag="ACG9CY_000174"
     CDS             3818..3934
                     /locus_tag="ACG9CY_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4936721.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4862..5200
                     /locus_tag="ACG9CY_000175"
     CDS             4862..5200
                     /locus_tag="ACG9CY_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936722.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5294..5524
                     /locus_tag="ACG9CY_000176"
     CDS             5294..5524
                     /locus_tag="ACG9CY_000176"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS4936723.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            5924..6295
                     /locus_tag="ACG9CY_000177"
     CDS             5924..6295
                     /locus_tag="ACG9CY_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS4936724.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6569..7261)
                     /locus_tag="ACG9CY_000178"
     CDS             complement(6569..7261)
                     /locus_tag="ACG9CY_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4936725.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7264..8049)
                     /locus_tag="ACG9CY_000179"
     CDS             complement(7264..8049)
                     /locus_tag="ACG9CY_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4936726.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8286..9686
                     /gene="lacG"
                     /locus_tag="ACG9CY_000180"
     CDS             8286..9686
                     /gene="lacG"
                     /locus_tag="ACG9CY_000180"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4936727.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9711..10028
                     /locus_tag="ACG9CY_000181"
     CDS             9711..10028
                     /locus_tag="ACG9CY_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4936728.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10041..11714
                     /locus_tag="ACG9CY_000182"
     CDS             10041..11714
                     /locus_tag="ACG9CY_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS4936729.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11728..13359
                     /locus_tag="ACG9CY_000183"
     CDS             11728..13359
                     /locus_tag="ACG9CY_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS4936730.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(13850..15415)
                     /gene="guaA"
                     /locus_tag="ACG9CY_000184"
     CDS             complement(13850..15415)
                     /gene="guaA"
                     /locus_tag="ACG9CY_000184"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4936731.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15474..16397)
                     /gene="coaA"
                     /locus_tag="ACG9CY_000185"
     CDS             complement(15474..16397)
                     /gene="coaA"
                     /locus_tag="ACG9CY_000185"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS4936732.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16593..17198
                     /locus_tag="ACG9CY_000186"
     CDS             16593..17198
                     /locus_tag="ACG9CY_000186"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4936733.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17506..18522
                     /locus_tag="ACG9CY_000187"
     CDS             17506..18522
                     /locus_tag="ACG9CY_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4936734.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18663..19964
                     /locus_tag="ACG9CY_000188"
     CDS             18663..19964
                     /locus_tag="ACG9CY_000188"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS4936735.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20010..20672
                     /gene="deoC"
                     /locus_tag="ACG9CY_000189"
     CDS             20010..20672
                     /gene="deoC"
                     /locus_tag="ACG9CY_000189"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS4936736.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20693..21091
                     /locus_tag="ACG9CY_000190"
     CDS             20693..21091
                     /locus_tag="ACG9CY_000190"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS4936737.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21157..22239
                     /locus_tag="ACG9CY_000191"
     CDS             21157..22239
                     /locus_tag="ACG9CY_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4936738.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22451..24013
                     /locus_tag="ACG9CY_000192"
     CDS             22451..24013
                     /locus_tag="ACG9CY_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4936739.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24006..25139
                     /locus_tag="ACG9CY_000193"
     CDS             24006..25139
                     /locus_tag="ACG9CY_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4936740.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25139..26095
                     /locus_tag="ACG9CY_000194"
     CDS             25139..26095
                     /locus_tag="ACG9CY_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4936741.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26194..27360
                     /gene="deoB"
                     /locus_tag="ACG9CY_000195"
     CDS             26194..27360
                     /gene="deoB"
                     /locus_tag="ACG9CY_000195"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS4936742.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27384..28202
                     /locus_tag="ACG9CY_000196"
     CDS             27384..28202
                     /locus_tag="ACG9CY_000196"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4936743.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28219..28926
                     /gene="deoD"
                     /locus_tag="ACG9CY_000197"
     CDS             28219..28926
                     /gene="deoD"
                     /locus_tag="ACG9CY_000197"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4936744.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29224..29910)
                     /gene="gpmA"
                     /locus_tag="ACG9CY_000198"
     CDS             complement(29224..29910)
                     /gene="gpmA"
                     /locus_tag="ACG9CY_000198"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4936745.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30082..30663)
                     /locus_tag="ACG9CY_000199"
     CDS             complement(30082..30663)
                     /locus_tag="ACG9CY_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4936746.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(30796..31479)
                     /gene="rpiA"
                     /locus_tag="ACG9CY_000200"
     CDS             complement(30796..31479)
                     /gene="rpiA"
                     /locus_tag="ACG9CY_000200"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS4936747.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            31798..32211
                     /gene="rpsL"
                     /locus_tag="ACG9CY_000201"
     CDS             31798..32211
                     /gene="rpsL"
                     /locus_tag="ACG9CY_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS4936748.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32295..32765
                     /gene="rpsG"
                     /locus_tag="ACG9CY_000202"
     CDS             32295..32765
                     /gene="rpsG"
                     /locus_tag="ACG9CY_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS4936749.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            32859..34946
                     /gene="fusA"
                     /locus_tag="ACG9CY_000203"
     CDS             32859..34946
                     /gene="fusA"
                     /locus_tag="ACG9CY_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS4936750.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35118..36305
                     /gene="tuf"
                     /locus_tag="ACG9CY_000204"
     CDS             35118..36305
                     /gene="tuf"
                     /locus_tag="ACG9CY_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4936751.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36712..37020
                     /gene="rpsJ"
                     /locus_tag="ACG9CY_000205"
     CDS             36712..37020
                     /gene="rpsJ"
                     /locus_tag="ACG9CY_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS4936752.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37051..37680
                     /gene="rplC"
                     /locus_tag="ACG9CY_000206"
     CDS             37051..37680
                     /gene="rplC"
                     /locus_tag="ACG9CY_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS4936753.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37708..38331
                     /gene="rplD"
                     /locus_tag="ACG9CY_000207"
     CDS             37708..38331
                     /gene="rplD"
                     /locus_tag="ACG9CY_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS4936754.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38331..38621
                     /gene="rplW"
                     /locus_tag="ACG9CY_000208"
     CDS             38331..38621
                     /gene="rplW"
                     /locus_tag="ACG9CY_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS4936755.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38660..39493
                     /gene="rplB"
                     /locus_tag="ACG9CY_000209"
     CDS             38660..39493
                     /gene="rplB"
                     /locus_tag="ACG9CY_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS4936756.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39536..39814
                     /gene="rpsS"
                     /locus_tag="ACG9CY_000210"
     CDS             39536..39814
                     /gene="rpsS"
                     /locus_tag="ACG9CY_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS4936757.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            39837..40184
                     /gene="rplV"
                     /locus_tag="ACG9CY_000211"
     CDS             39837..40184
                     /gene="rplV"
                     /locus_tag="ACG9CY_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS4936758.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40198..40854
                     /gene="rpsC"
                     /locus_tag="ACG9CY_000212"
     CDS             40198..40854
                     /gene="rpsC"
                     /locus_tag="ACG9CY_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS4936759.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            40857..41291
                     /gene="rplP"
                     /locus_tag="ACG9CY_000213"
     CDS             40857..41291
                     /gene="rplP"
                     /locus_tag="ACG9CY_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS4936760.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41281..41469
                     /gene="rpmC"
                     /locus_tag="ACG9CY_000214"
     CDS             41281..41469
                     /gene="rpmC"
                     /locus_tag="ACG9CY_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS4936761.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41494..41760
                     /gene="rpsQ"
                     /locus_tag="ACG9CY_000215"
     CDS             41494..41760
                     /gene="rpsQ"
                     /locus_tag="ACG9CY_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS4936762.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            41818..42186
                     /gene="rplN"
                     /locus_tag="ACG9CY_000216"
     CDS             41818..42186
                     /gene="rplN"
                     /locus_tag="ACG9CY_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS4936763.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42223..42531
                     /gene="rplX"
                     /locus_tag="ACG9CY_000217"
     CDS             42223..42531
                     /gene="rplX"
                     /locus_tag="ACG9CY_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS4936764.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42559..43098
                     /gene="rplE"
                     /locus_tag="ACG9CY_000218"
     CDS             42559..43098
                     /gene="rplE"
                     /locus_tag="ACG9CY_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS4936765.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43117..43302
                     /locus_tag="ACG9CY_000219"
     CDS             43117..43302
                     /locus_tag="ACG9CY_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS4936766.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43338..43736
                     /gene="rpsH"
                     /locus_tag="ACG9CY_000220"
     CDS             43338..43736
                     /gene="rpsH"
                     /locus_tag="ACG9CY_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS4936767.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            43768..44304
                     /gene="rplF"
                     /locus_tag="ACG9CY_000221"
     CDS             43768..44304
                     /gene="rplF"
                     /locus_tag="ACG9CY_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS4936768.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44465..44821
                     /gene="rplR"
                     /locus_tag="ACG9CY_000222"
     CDS             44465..44821
                     /gene="rplR"
                     /locus_tag="ACG9CY_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS4936769.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            44842..45342
                     /gene="rpsE"
                     /locus_tag="ACG9CY_000223"
     CDS             44842..45342
                     /gene="rpsE"
                     /locus_tag="ACG9CY_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS4936770.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45357..45536
                     /gene="rpmD"
                     /locus_tag="ACG9CY_000224"
     CDS             45357..45536
                     /gene="rpmD"
                     /locus_tag="ACG9CY_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS4936771.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45580..46020
                     /gene="rplO"
                     /locus_tag="ACG9CY_000225"
     CDS             45580..46020
                     /gene="rplO"
                     /locus_tag="ACG9CY_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS4936772.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46020..47315
                     /gene="secY"
                     /locus_tag="ACG9CY_000226"
     CDS             46020..47315
                     /gene="secY"
                     /locus_tag="ACG9CY_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS4936773.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47377..48024
                     /locus_tag="ACG9CY_000227"
     CDS             47377..48024
                     /locus_tag="ACG9CY_000227"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS4936774.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48215..48433
                     /gene="infA"
                     /locus_tag="ACG9CY_000228"
     CDS             48215..48433
                     /gene="infA"
                     /locus_tag="ACG9CY_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS4936775.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48466..48582
                     /gene="rpmJ"
                     /locus_tag="ACG9CY_000229"
     CDS             48466..48582
                     /gene="rpmJ"
                     /locus_tag="ACG9CY_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS4936776.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48600..48965
                     /gene="rpsM"
                     /locus_tag="ACG9CY_000230"
     CDS             48600..48965
                     /gene="rpsM"
                     /locus_tag="ACG9CY_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS4936777.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            48993..49382
                     /gene="rpsK"
                     /locus_tag="ACG9CY_000231"
     CDS             48993..49382
                     /gene="rpsK"
                     /locus_tag="ACG9CY_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS4936778.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49462..50400
                     /locus_tag="ACG9CY_000232"
     CDS             49462..50400
                     /locus_tag="ACG9CY_000232"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS4936779.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50428..50808
                     /gene="rplQ"
                     /locus_tag="ACG9CY_000233"
     CDS             50428..50808
                     /gene="rplQ"
                     /locus_tag="ACG9CY_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS4936780.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51080..51919
                     /locus_tag="ACG9CY_000234"
     CDS             51080..51919
                     /locus_tag="ACG9CY_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4936781.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            51895..52764
                     /locus_tag="ACG9CY_000235"
     CDS             51895..52764
                     /locus_tag="ACG9CY_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4936782.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            52754..53551
                     /locus_tag="ACG9CY_000236"
     CDS             52754..53551
                     /locus_tag="ACG9CY_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4936783.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            53754..54500
                     /gene="truA"
                     /locus_tag="ACG9CY_000237"
     CDS             53754..54500
                     /gene="truA"
                     /locus_tag="ACG9CY_000237"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4936784.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            55164..55358
                     /locus_tag="ACG9CY_000238"
                     /pseudo
     CDS             55164..55358
                     /locus_tag="ACG9CY_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55546..56322)
                     /locus_tag="ACG9CY_000239"
     CDS             complement(55546..56322)
                     /locus_tag="ACG9CY_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4936785.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT        18024 a        10109 c        12076 g        16301 t
ORIGIN      
        1 gacggaattc ctttctagat tgagatattg actaggaaga atgttaagat tttaagcctt
       61 taagctaaaa aaaaaccctt tttgttaaat aaggggaata tagcctgtca gtttcaaact
      121 gcaagtcgtc ctgctgcaat aatatagtag attagagatg aaaatagatt tttttaaacc
      181 aatgactgaa taaaaagacc gtcatagata cacattggaa tgtgtctatt acggtctttt
      241 tttacgaacg attttattga agacggacag ttcgggtcaa aaataaaaaa gctacgatca
      301 gaaataagaa taaaatgtat cccaaaataa tggctagacg gtctaactta tttgtcgata
      361 gatgtttgtt tagaagttga taggcccata aacctatcag tcctcctgtt gtattagtta
      421 tcaaatcagt gatgtccgct acgccaatac cgaataggaa ttgaatcaat tctgccaaga
      481 agcttacaag gaaaataatc cctaattttt tagaaaaaga aagtctcttg aaattgacat
      541 tcaaaagcac cccaaaagga aaaaagacaa tcaagttata agtggtctca cgtaataccg
      601 tcgtattttc aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg taagaataag
      661 tgagaaattg tggaagatta aatgatagct tgaataaaat caaccataat agaaagaata
      721 aataaatccc taataaaaat tttgatacat accgcataga catctcctcc atttaaaaag
      781 tataccatcg aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg accaacgtag
      841 ttatcgtacg aaaattagaa gctttttttt gttgggaatc cggtttcttt aacaaagcta
      901 agatagcgag ttgcttggaa aagttgctat cttttttctg tcaaaacgac tcggaatgtt
      961 cgttttttgt tctaatttct ccaagttgtt tactttttga tactaagttt atgaaaaatc
     1021 aagaaatagt tgttatacta ggtaatgctg acaaacggga ttggcatttc atcttggttc
     1081 agcttttttt ccaattacta ggaaggaggg atagattgtt caagacttat tttcgtttca
     1141 ataaaataca agataatcct tttcgtatca tgaatctgat gctacttatc tttattgtat
     1201 tatggttagg ttacataaat catgattttt tgatttcaag tttttcatca ttagggatct
     1261 ttactttttt ctattatcaa aacataccga tgaaatattt gctgaaacgt atgtttctga
     1321 tcggttgcgg actgttggtt gctttcgttt tggggatctt atcgacttat gttttatggc
     1381 ttgaaccatt tgccgtagct ttggttgctt tttccagcag attcgtgttg cgattattcc
     1441 atatttctaa accaggtggc ttgttttttg cgatgctttc tgccatggga acgagcatgc
     1501 agctgccaat cgctcaatta ccgatcgtct cactttattt ttttatgggt gtcgtttttg
     1561 ccctgattgc ggcagtgatt acaaaattac tggattcacg gcctgagcag acaattgaaa
     1621 aagcaacttt aaaagaaaga tttcacgaag agcctttagt gatcattgat tctgttttct
     1681 acagcgctgc cttatttctt tccgtttatg tcagtcacgg cttgaatctg cataatcctt
     1741 attggttgac cttgtcatgt gcatcgattt tgctagcaga aaacttggat gcgatgaagc
     1801 atcgacaagt ccaatatctg attggatcga tgggtgggtt atgtgtgtcg gcattcttgt
     1861 catttgtgcc gtttacgcag ttgcagacca tttttctgat cacttttcta tatggaatcg
     1921 cacaatttct agttgctaga aattatgcag ttgctaatat ttttcttaat ccgatggcgt
     1981 tgatgctatc tacacttatt cgtggagcat acttgatttc tttgatcgaa tatcgttttt
     2041 tagggatcgt cataggaagt ttcatcggtt taggtgttgc gtgggtaatg acggttggtt
     2101 tgcaacatta tttaacggtt gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc
     2161 aaaagaggtt atgacaagat ttttgtcaca acctctaatt caaggtgttc aaaagccaga
     2221 ttctcagcat taagtcaaat tcacacaaaa atgtggaaag gaactttcaa aaaaatttta
     2281 tatttattct atcaaaaaat tttgtttatg atggtaactg aataaacttt cctgagatgg
     2341 tcaatgcttt ccttgaaagt cttacagata ttctttcttc atagaaagtt taagttttat
     2401 agttagtgta caataaaagt atgtttatac catatggtgt ccggataatc agtcatttaa
     2461 acaatgaggt tcaaagatat gaaaagggga aatttgatga agaaaataag caatgaaatc
     2521 caaattctgg cagaaacttt aactcttccc aaagtcgtta tagagcaaat gattgtacaa
     2581 gcaaaaaaca tccttgatga ggtgtttgag caatatagtc atgaactaat ggatcctaat
     2641 aaagcaaagg cgactgcaaa gaaaatagac aaagtctatg aagaggaacc ttttgcagca
     2701 ttagctattt atctatatgc agcttctcaa agttggaaaa aaatttatca accattgaag
     2761 attccaagaa ctatctattt ggcaacaatg aatgctttta ctagatttat tcatgaacat
     2821 ttccaagtaa caggaagcta tcgctttgac cgtggttttt ggatttggca ttacactagt
     2881 ggcttgatct ttcggattgg tgaattggag tacgaaatga catattttcc gaaagggtac
     2941 acggatctaa gattagaagg aaagcagtgc ctttctcttc atattccttc cgatgcagat
     3001 ttatctcaga ataagataag agatagttac gaaagtgcca ttcgtttttt tgaactttat
     3061 tttcctgatt accattacca agtgatgtac acagatactt ggttactatc acccaatctt
     3121 actaaatggt tgaaaaaaga gtcaaaaatc tgtctttttg cagccgatta tcgattatta
     3181 tcagttgatg aacaggacga tagtggagtc ccttggatct ttggacgagt agatgcccaa
     3241 atacatgatt atccagaaag tacgagttta cagcgacaag caaaagagca attgcttgca
     3301 ggcgagcaca ttggctctgg cttaggtatt cttacttcat aagtttagta cttcgaaata
     3361 gtttaaaaga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
     3421 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa
     3481 agcaattttc gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
     3541 ttttagtgtt ctatcgaaca cacagtacgt tcaataggta aactagctat aacaatagat
     3601 attaaagtta acggacaaga gggccttttt taggtagcta aaacgtaata atgaaaattt
     3661 atttccagag ataggagtca atcagctagt ctgatcgact gttttaacaa gttgactttc
     3721 attttaactt ttgataccat ttaaaacaca aatcgtaatt gtttcgattt gtgtttttta
     3781 gcatgactac aatcaaagaa gaaagtgggt cgtccatatg gaaaaaacag atatctcaag
     3841 tgcataccgc cgcttgaaaa gcccaaatat caaaacacga aaacgtgcgc tgaagatcat
     3901 caaagatgtg aaacggaata gtgggaaaag gtaagaagaa ggaaacttaa ttaaaaaaat
     3961 agccgaaaaa cgtttttcgg ctattttcaa taatcggttt ttgtgtaagg tttccgaaag
     4021 aactgctgtt gattttccat ttttaacata tgataaattg gtagaatctt tcttttcaaa
     4081 ttgataccat tccctaattc cagttatata gttaactaca gaaccacaaa gcacgtacgt
     4141 ttataaaagt attatctgta gttatttttc aattcccttt caacctctta ttttgaccag
     4201 tagtattgat cactttacct tttaacacct ttgttcgttt ttgaaacatt tttagtcaag
     4261 aatggaaacg taactacata atcaaaggtt ttgcgaaata tttcatgaaa tatttcatgt
     4321 agaagcgagc aatacggttg caaataacca ttatcgttta ataagcagtt acgaaagctc
     4381 ccaatatctc taacgttcag ctcactccat ttcttttctt caaaaaaatc agtgattcgt
     4441 atctctttct atttgtcgaa aaagttaact ttaaaaagta acgccgaaaa aatatgcaaa
     4501 aaaataaaaa agcagttact attgctagaa taacatcaag gaataataag ttttggtttt
     4561 cagataatag tcgataagtt gtgtattgga attttagata cagtaataaa gtatttttca
     4621 tgaaattttg tgagaatcac tattacaagt atctttaata aaattaatgg ttaaggagga
     4681 acttaaagta gaatgtaaat gaatagttgt agtcagtcga ttgtgatact agactcaaag
     4741 ttgcaggaat aagtttttca atttgtgatt ggaagataaa aaatgaaaaa gaagggcttt
     4801 tttttatact ttgttgattg tagtgtttat tttccctctg tttagccaag taacattttc
     4861 tatgactcaa ccagtttcaa gtgaggaaca tgataacctt tttcgtgcta cagaagagtt
     4921 taagaaatat caggaaaata ttgttccgga gactccatta gctagtgagc caattactga
     4981 tattgatgga aaccaaatag gatggtatat acagtacgaa gttatggtgg taaataatga
     5041 ctcaatagta aaaaatacag taggcttaga gctaacaccg attgaaaagg tgagttcatt
     5101 gttaacgtat atttatactg atgaaagtga cgagactgag atcattttaa cggattactc
     5161 aaaaattgct actgataaaa agtatatatt gtggatgtag acactagtga aatactttat
     5221 gatatagaaa ttgatgaaaa tttactagat gtagcacaag aaatcgatca tcaaaagcag
     5281 gaagctattc gatatgctgg tagaaaaggg aaaagaaaat atttattacg tgaatgtagc
     5341 aaaagttaga gaagatgaaa atgagtggaa agaattcaag agtcgctact caattaactc
     5401 gactccgaca tttacagttt acagagaagg atcgatagaa aaaactgttt tttggactaa
     5461 agaaagtgga atgtctttag ctgaagtaga agagtttttg gattatgtta gtatgcaaca
     5521 gtaagggagt tcttattatg aaaaattttg acacatatta tgttattgtt ttttcgatat
     5581 ctttgctgtt aatattacta actttaatgc agtataaaga gtatccagag tattttattt
     5641 taacttttgt atatttagta agtagctaca cacttctata taaattcaga agttcttcta
     5701 gagtttctaa agtttgtttc ttaattagct tattgattct ataatatttt tgatttatat
     5761 aggaaattaa aaatattata gaatcaacac tcagagaacg ggttggtttt taatagattt
     5821 aataagtact gataatgaaa tttctcctgg ttgggaaaac gaagttggtt accctgtaga
     5881 agaatttttt caagattact ttgcggagta tgccgatagg ataatgagag attacgtaac
     5941 agataaaact caagaagtca tcaatcaatc ccagtcatta aatgaattat ctgataaggc
     6001 gaaagaagaa gcagttgcca ttatttcttc agggattagt gactatattg atatgagtgg
     6061 tgaaatcaat tatggcgata aagacaaaat tgtacgcatg ataaaagctt ttatcaaaac
     6121 ttttgatgtg aaggcagatc ttaagtttaa tgatacttac ctagtaggaa aattaattaa
     6181 tatccttggg acagagaaag atacggaaac gtttatttcc tctttgtcta tgttgtttac
     6241 agataagcat ttttcaactc agcttggtgg agaggaacta gtggaaatct tttaaccatt
     6301 acgcccagca ttaataaaag tatatcaaaa aagtactcct gatgattttt aagattggtt
     6361 tgagaaataa tgtgaaaaca ctatttttaa tgttaattat agatagagat gaacgcgtca
     6421 tatggttaaa agaatgaaaa agtcaaagat acactgctat atttcactaa ggatatagca
     6481 gtgtatcttt catgtcgttt cactggcagt tttttataaa aggaggtgtg catttcagtt
     6541 gcttttcttt ggtagttagt tgaatcgttt aagaattaac ggatgtggca gacaggtgct
     6601 ctgtccaaaa accagtaaaa tctttgatgg ttccatcttt atttagctgt gcctgttcaa
     6661 atactgtttt gtttttaccg aatggatcaa ttgcaataat tgttccgatc cccgcacgtt
     6721 tggcagcaag taatcctgag taagcatctt cgataacaag acaatcagcc ggagctaagc
     6781 tcagtttgtc tgccgcttta agaaaaatat ccggttgcgg tttcccaggg aaagagccgt
     6841 cgtcatagac aatcttgtcc cagtcaaacc agcgggctaa atcgaaaata tcaaaataaa
     6901 aagcaacatt ctctttgaca gtagccgtgg caatcgttaa tggaatctgt tggtcagtca
     6961 gctggtcaag agtcgcttca agcccttttg tcaactgaag ctcgttttca ttttgcaagc
     7021 aaagcatacg ataatagctt tctttttctt ctgacaatac ggcgatctca gaggcggtca
     7081 aatgctctga aatgaaatgt ttcaaaatct ccgtattcgt ccgaccatga atatttttta
     7141 aaatatcctc gtctgataaa ccatcttttg tatgatcatg tatcatatgc agccaagcag
     7201 cttcatgcag atgagagtcc aaaaacattg ttccgttgaa atcaaatata atccctttca
     7261 tagttacgct cctatagtgg tattttcggt tgaatcagca atttgcagat cgacatattg
     7321 tgcatagtgc tcttgaacct cccgcgagat ctggcgatcc gtaatgagca gatcgaaatc
     7381 atgcaaatta taaaacacat aaaaatcatc tcgattgaat tttttattat caacgagtag
     7441 atatttctta cgggaattat ttagagcgat ctgctgtgct tctccttctt cggtactgta
     7501 agtagagatc gcttcgttgt gaacgccatt tgaactgata aatgcttttg taaactttag
     7561 tcttcttaaa ttgtcgttgg tgatcggtcc gacaaatgcg ccggtatttt tccggaaatc
     7621 accgccaatc agtagaattt ctggtccatc tgcttgagca agaatatcaa aaactggata
     7681 attatttgta atgatccgaa ttttcttgtt cagtaaatgc gtcgccaata gttcgattgt
     7741 tgtacctggt cctaagaaaa tcgtttctcc ctcgtgaatc aaggaggctg ctgtggcagc
     7801 aatttctttt ttttcttgaa tctgcagagt ggctttttca gtgtgagaaa gttcttgatc
     7861 taaagtgtag gaaagacttt gagcccctcc atggacacgc agcagcttgc ccgatttttc
     7921 taattcatct aaatctctgc gaacggtcat atcagaaaca tctaattgat tgatgatttc
     7981 attaacggta aggattcctt ttttattgac catttctacg atagtaagca atctttcttt
     8041 tttcaacatt gtcttcacct ctaatttaag gataatctat catataaacg gacaacagtc
     8101 aatctaattc gaacaaaagt aaacatttag ggtggttttt atgtttgtta caatagattt
     8161 tattgctgtt ttctggtttt tatgattatt tgtgtttgtt tttgttgaca aatgttttgg
     8221 atgcgtttac aatgcaatca gttagaaaga gcgaaaactg attatagatt tgaggagaga
     8281 tgtacatgct taaattacca gaagatttta tttttggcgg agccacagca gcctatcagg
     8341 ttgaaggagc cactaaggaa ggtggcaaag gcgctgttgc ttgggatgac tttttagagg
     8401 aacaagggcg attcagtccc gatccggcaa gtgattttta tcatcaatat gcaaaggaca
     8461 ttgaattgtg tgaacgtttt ggtgtaaatg gtctaagatt atccattgct tggagccgca
     8521 tttttcctga tggagctggg aaaccaaatc cagaaggaat cgctttttat catcgtgtgt
     8581 tcgaagaatg taagaaaaga aacgtaacgc cgtttgtgac gttgcatcat tttgataccc
     8641 ctaaacgttt gtttgaccat ggagattttt tgaatcgaga aacaatagaa gcatttgttt
     8701 cctatgccat tttttgtttt catgaattca aagaagtcaa ggtgtggagt acctttaatg
     8761 aaatctatcc agttgcaacc aatcaatatt tgttaggtgt tttcccacca ggaatcaaat
     8821 atgattttac aaagatcgtc gcctgtcttc ataatatgat ggttgctcat gcccgtgtag
     8881 tgaactattt caaagaaaac gaacttccgg gtgaaattgg tgtagttcac tcattagaga
     8941 cgaaatatgc ggcaacagac gcgccggaag ataagcatgc tgccttttta gacgatgcat
     9001 tgtcgatccg tttcttatta gatgctacct atttaggcta ttattcaact gaaaccttga
     9061 ctgctcttga tgagatatgt gaagcaaatc aggccagcta tcattttcct gaagaagatt
     9121 tcgttgaatt gaaaaaagca tccacgcgta atgactactt agggatcaat cactaccaat
     9181 gccattttgt caaagcttac gatggagaaa atgcaatcca tcataacggt acgggtgaaa
     9241 aagggacctc tgtttataaa gtgaagggaa tcggagaacg tatttataaa gaaggaattc
     9301 ctcgaactga ttgggactgg ctgatttatc cagaaggtct atatgacttg ttgctgcgaa
     9361 ttaaaagcga ttacccacat tacaacaaaa tctatatcac tgagaacggg atggggtata
     9421 aagatcagtt tgaagatggc attattatgg atcagccgag aatcgactat ttgagagtct
     9481 atttggaatc cttaagcaag gcgataacag ccggggtaaa tgtgaagggg tattttttgt
     9541 ggtcgcttat ggatcttttc tcatggacaa atgggtataa caaaaggtat ggcttgtttt
     9601 atgttgattt tgaaacccag aagcgttacc caaaagaatc agcttactgg tacaagttgg
     9661 tcagtgaaac caaaacgatt atttaataag tgataatgga ggaagaaaag atgaataaag
     9721 aggaattaca aatgcttggt tttgagatcg ttgcatattc aggcgacgct cgcagtacat
     9781 tattgaaatt attgaaagaa gtaagacaag gaaactttga acatgttgat tcggcgttga
     9841 aagaagccga cgaaaattta acccttgcac ataattcgca aacaaaaatt ttagcggaag
     9901 aagcatctgg gaaagagatg gagatgggat tcatttttat ccatgggcag gatcatttaa
     9961 tgaccacttt attgctgaga gatctgattc aagactttat tgtactgtac agacagaatc
    10021 aaggatagga gaagaacgct atggatggaa tcattaaaca aatcgaaaaa gggaaaccat
    10081 tttttgaaaa aatctcacgt aatagttatt taggagccgt tcgtgacgga tttttaacag
    10141 cgatgccagc tattttattt tcaagcattt tcattttgat tgctgcggtt cctgagattt
    10201 ttggttacca atggcctgaa tctgtctcca catggctatg gaaagtctat aattattcga
    10261 tgggtgttgt agggttactc gtttcagcaa ccactgctcg ctgcttggct gattcgatga
    10321 atcgtcgctt accgaaaaac aaagtaatca acaccacctc tgtgatgctg gcctctgtgg
    10381 taagtttcct gttgttaagc gtctctccga ttgaaggtgg attttcaacg gaatatatgg
    10441 ggacaaaggg actactggct tcctttgtcg ctgcattttt aacagttaat ttatataagt
    10501 tttgtgtgct gaaagatatt accattaaaa tgcctaaaga agttcctgga acgatttcgc
    10561 aaaccttccg agatattttt cctttctctt tttcagtatt tgcggccgtt ttaattgatt
    10621 taggcattcg acaaatcttc caaatctcat ttgcagaagc aatcattacg gtgctgcagc
    10681 cgctatttac agcagctgac gggtacttag gaatcgctat tatctgggga gcgatggcgc
    10741 tgttttggtt tgtcggtgtt catggaccgt cgatcgtgga gccggcaatt gcggcaatta
    10801 tctatgccaa tgtggaaacc aatctgcaat tgttcaattc cggtgaacaa gcttctaaag
    10861 tactgacggt gggtttaggg aattttgtag gaactatggg aggaactggg gcaacattgg
    10921 ttgttccttt tatcttccta ctctttgcta agtctaagca gttgaaggca gttgggaaag
    10981 cttcctttat tccagtaagt ttcgctgtca acgaaccatt gctatttgcg gcacccatga
    11041 ttttgaaccc ttattttttt attccatttt tgttaacacc tgttgtaaac gtttgcctgt
    11101 tcaagttttt tgtcgatgta ttgggcatga acagctttat gtacgttttg ccttgggcaa
    11161 ctccagcacc gatcggtttg gttctaggaa cgggcatggg tatcttagct tttgttctag
    11221 cagcggttct aatcgtcgtt gacttcctga tctatctacc attttgcaaa gcatacgatg
    11281 ctgtcttagt agagaaagaa gcacaaaaag cagaggtgat cgcaaccgag gaaggtgctt
    11341 ctgccgatgt acgtgaggaa gtacctgtgg cagcgagtca agttatcagc gatgcaaata
    11401 aagaaatcaa tgtgttggta ctttgtgctg gcgcagggac aagtgcgatg ttagctaatg
    11461 ctttaaaaaa aggtgcatct gaatcgaaag caccgatcag tgcaatggcg ggtgcttatg
    11521 gttctcatta cgaaatgctg caggattttg atatggtggt cttagcaccg caagtcaatt
    11581 cttactatga tgatatcaaa gaagacaccg atcgcttagg aatcaagctg gttgcgacaa
    11641 aaggagcaga atatatcgga ttaacgagag atcctgaaaa agctgttcag tttattctga
    11701 accaatttca ataaaggagg agccataatg aaaatgggaa tttggttgcg taaagctgtt
    11761 ttatttgcag ctgtgttagg ggctttgggg gctgtttcaa aaactgttga agcatctgcc
    11821 agcgaattta ttcgcggagc agatatttcg atcctgtcag atatggaaaa atctggtgca
    11881 aaatattatg aagacgatat ttcaaaagat gccttagaga ttttaaaaaa taatggtgtc
    11941 aactatgttc gactgcgttt gtggcaggat ccttataata gtaatggaga aagttatgga
    12001 gcagggacaa atgatctgga aacgacgatt gaccttgcaa aacgagcaaa aaatctggga
    12061 cttaaggtgc tgctggattt tcactacagt gatttttggg ttgatcctgg gaaacagaat
    12121 cttcctaaag catggcaagg tctgaccttt gaagaaatga atacggcatt gtacgactat
    12181 acaaagaacg ttctgtcgga aatgaagcag ctggacgttt atcctgatat ggttcagatc
    12241 ggcaatgaac tgaatagcgg aatgctctgg ccttatggca agtcttgggg agaaggcggg
    12301 ggagaatttg atcgattggc tgcttttttg aagtctggta tccaagctgt tcgagacact
    12361 caacctaaaa caactccagt aatgctccac ttagctgatg gtggcgatac aggagcattt
    12421 acttggtggt ttgatgaaat cacgagtcga ggtgtgtcat ttgatttaat tggagtgtcg
    12481 tattatccgt attggcacgg ttcaatgaat gaacttcaaa ccaatatgaa tgcgatcagc
    12541 caacggtatg gtaaagaagt gatcgtagtt gaaacagctt atgggcatac aacagcgaat
    12601 gccgatacta tgccaaatgc ttttggtgaa gccgaagcag ctgcgggggg atatgaacca
    12661 acaccgacag gacaagcaga gtatttgctg gatcttgccg atcggattca ggcagtaccg
    12721 aataatcgcg gggcgggctt cttctattgg gaacctcttt ggtacaacgg aaatgtgagt
    12781 tgggcaacac aagcaggtat gaattatctc agtgttcaat ccactatggg aaacgaatgg
    12841 gacaaccaag cgatgtttga ttttgctgga aacgccttgc caagcttgcg tgcgtttaaa
    12901 caagcagggg cgcaaataaa tctagttaaa aatgcaagtt tcgaaagcga tggaccaacg
    12961 accagtcctt ccaattggca aacctggttt caacaaggaa ccgctaaggg tacgatcaaa
    13021 acggaagcgg gcactttttt tggggactac aaactaactt tttggcagga tcaagcatac
    13081 gaggcatcag cttatcaaaa tgttagtggc ataagcaaag gaacttacac attgagtgcc
    13141 tatgtaatgt ctgggggaga gtttgatact aatcagttat atatcaaaaa ttatggtgga
    13201 gcggaactaa atactgcggt tgttaaaaca agtgaatgga caaaaattac aatcaaagac
    13261 attccaatta caacaggtgt ttgtgaaatt ggggtttata gcaaggcttc aggaaatgct
    13321 tggacgaacg ttgattttgt ttcattggtg aaacaataga ctgcggatcg tttcgattca
    13381 agatcggagg ttcccttcag ccacatcgtt agcgtagaag gcaggaatcg aacctgcacc
    13441 tcgcctttgc caaaaataac cagaaacgat ttccagtcat ttttaccaaa acgagtaaag
    13501 caccagactt catctacact ttgtattatg ctaaagagag ttcaacgatt tcgtaagaag
    13561 caatgaaaaa attgcaaaaa gttgtgcaga taacataaag tttgtttaag aaagggtaac
    13621 cttagaattc cgtcgtgaat atgatttgag cacttagatg gatagaaaaa agttctcgga
    13681 aaacagttcg tttttcgaga acttcttttt atgataagaa tgaaacaaat taacttatat
    13741 tatagtctct tttggtaggg aaccatctta tttttaatga aaatcaagca ttttagctat
    13801 tgaagaatgt tgatttatag ggattccccc tccatattat gttggctctt tattcccact
    13861 ccactgttgc tggtggttta gaagtgatgt catacacgat acggttgacg tgggctactt
    13921 cgtttacgat acgtacagag attttttgca agacttccca tgggatacgg gcaaagtcag
    13981 cggtcattcc gtcgattgaa gttacagcac ggataccgac tgtgtagtcg taagtacgtc
    14041 cgtcacccat aacaccgaca gagcggatcc ctggaagaac agtgaaatat tgccagatat
    14101 cacggtcaag tccagcagct gcgatttctt cacgcaagat agcatcagat tcgcgtacga
    14161 tttctagctt gtcttcagtg atttcgccaa gaacacgaat accaagacca ggaccaggga
    14221 atggctgacg ccaaacgatt gaatcaggca tgcctaactg agtccctaat tcacggactt
    14281 cgtctttgaa taatgtgttt aatggttcga tcaattcaaa ttgcatatct tctggcagtc
    14341 cacctacatt atggtgagat ttgatcgtct gtgctgtttc tgtaccagat tcgataacgt
    14401 cagtatacaa cgttccttgt gctaggaaag agacgccttc ttcaccagca agttttgttg
    14461 cttcgtcatc aaaaacatat acgaattcat tcccaatgat tttacgtttt tgttctgggt
    14521 cagatacacc tttcaacttg tctaagaagc gatctcttgc attgactttg atgatgttca
    14581 agccgaattt tccgcctaga ctttccatta cttgttccgc ttcgcctttg cgtagcagac
    14641 cgtggtcaac aaagatagat gtcaattggt caccgatcgc tttttgtaaa agaaccccaa
    14701 cgacactaga atcaacgcca cctgaaagac caagcaagac ttttttgtca ccaacttgtt
    14761 cacggatttt ttctacttgc atatcgataa agttatccat tgtccagtta ccttcacagt
    14821 gacaaacatc aaaggcaaag tggcgcagca attcgattcc gtattcggaa tgacgtactt
    14881 ctgcatggaa ttggatacca taaaaattac gtgttgtgtc ttggatagaa gcaatcggac
    14941 aatctttgct tgttccgact gtttcaaagc cttcaggaac tttagttacc aagtcaccat
    15001 ggctcatcca tactgtttgt tgttttggtg tttctttaaa caagacagct tgatcgcttg
    15061 ttacttctaa caaggctttc ccatattcgc ggtttttagc aggctctacg gaaccgccaa
    15121 gtttgtacgt gatcaattgc ataccatagc aaatccctaa tacaggaata cctaaattga
    15181 agatctcagg atcgatccca aatgcattct catcatatac actgtttggt ccgccagaaa
    15241 gaatgattcc ctttggattc atcgctttta cttcttctgc agtaatgcgg tggctcaata
    15301 attcggaaaa gactccaaga tcacgaatgc gtcttgtgat caattggtta tactgactac
    15361 cgtagtctaa tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt gtcaaattta
    15421 ttacccctat ccctttaaat tttatctcaa aatagtcaaa ctattttaaa atgctaatat
    15481 tttcgcatga aaatcgaatc gatgatatga ttttcagttt tatggagaat tatatcggct
    15541 cttcctcgtg taggcaagat atattcctgt aaatttttca aattcacatt tttccataca
    15601 tcttttgcca ttttaaaggc ttcttcacga tctccgatag caaaacgata ataataattc
    15661 tctggatttt ggaaagccgt atccagtaac gcaccaaaac gatccaaata ccatttttcg
    15721 atcagcacgg gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct gacgtagatt
    15781 tgctgattcg caggtaattg aagcgtattg atcccctcaa cgatcaagat atcaggctgc
    15841 tggatcaatt cgtacttgtc tggaatgata tcatacacat catgtgagta aacaggtact
    15901 tttatctctt cctgaccaga ttttacacgg ttcaagaaat cgatcagcat ctccatatca
    15961 taactttcag gaaatccttt acggtccatg attccacgct cttttaatat acgatttgga
    16021 taaagaaatc cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt acgagacaaa
    16081 atcgtctgca gcagtctggc tgttgtactt tttccaacgg ctacacttcc ggcgatcccg
    16141 ataataaaag gcggtacttc tacgtattta tgaagaaata aacctttact caagaacaaa
    16201 gattcaaatt ctttcatata aagataaatc agatgagcaa gaggtacata gatatctcga
    16261 acatcctgca tggaaatgcg gtcattcaaa cttttgatat tatctagttc atcttgtgtc
    16321 aaaggtgctt gcccattacg ataaaattct cgccattctt ctcttgccac acggtagtag
    16381 ttcattggat cattcatatt agcctcctgc gttctcccat caatggaact attttaacac
    16441 aaaataaacg caaaccccac cacttaagag agattgccga tctttttgta taatctttat
    16501 aatagaaaga aacggagtct tattcttgac ttgcgcacgg atacataaaa ggatagactg
    16561 ttgtcatgaa agaaatgaga aggatggaag acatgacaaa tcattactat tctgaacgcc
    16621 cagatacggc gcacgatttt gaacaatggg cttttgagtt aaaaggcaga acctttcaat
    16681 ttgtaacaga ttctggtgta ttttcacgtg aaacagtgga ttatggttca cgtgtcctga
    16741 ttgatgcttt cgattgggaa gagttacctg atgaaggaaa gatcttagat gtgggatgcg
    16801 ggtatggacc gattgggtta gctttggcat ttgcgacaca gcggtttgtt gaaatggtcg
    16861 acattaatga acgagcggta agtctagcgc aaggaaacgc aaagcgtaac caaatcgaac
    16921 atgttgatat ccatcaatcg aatatctatg aagcagttca tgaagaaacc tatgcagcta
    16981 tcgtaagcaa tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt ttgacaggcg
    17041 catatcctcg gttgaagaag ggcggtacac tgacaattgt gatccagaag aaacaaggag
    17101 cgccgagtgc gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc gtcacaaaag
    17161 ataaaggata ttatatcatc aaaagtgtga aagaataact agctgaaaaa tagtggagcg
    17221 acgaaagagt ggaactgggc gtgcatgtac gggtacgtat cagttctttt tttgttgaga
    17281 agtttttaaa aacaaagaac tattttttct tcaaaaagaa atagtaggaa cagagtcgtt
    17341 gttttttacg ataaaataaa taagatacag aacttgtata gaagactgcc aagtcggtag
    17401 ataaaggagg tttttatcaa aaaacatact ttcctttttt ctctattctt ttcttggggg
    17461 ttttggtaca ctgataggga atttagatag aagggagcca ataggatgaa gctgaccatc
    17521 agagatatcg cagaaatggc aggcgtatca gtaacaactg tctcacagat tttgaataac
    17581 aaaggaagca gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat
    17641 cattataagc ccgattattt cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga
    17701 atgatcgtcc ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg tgtagagagc
    17761 tatttgaatc ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat
    17821 gagatcaagt atttacaaga gctttcccac cgctcagtgg acggaatctt gctggccaca
    17881 ccaaatattt tacctgagaa atatgcatta gacagcggtt tttatcaaaa aatgccaatc
    17941 attttaatcg atcgcggatt gaatcgtcgg gataacggac ggcttatcgt aaaggaatat
    18001 gagggcgcct atcaagctgt ttcgctgttt attgaaaatg gccatacgaa gatcggcatg
    18061 ctgaaggaaa ccactggata ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg
    18121 ctgaaagatc atggcctgcc ttttaatggg aaatttgttg aacatggaga gttgactgtc
    18181 caaggtggat atactgcatc aaaaaaatta ctcaaacata aagatatcac ggctattttt
    18241 tgcgggaatg atgccatggc gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa
    18301 atcccagaag atatctcagt cataggattc gatggattaa agctgtcaga atacatgatt
    18361 ccgcaattga cgacagtgca gcagccaagt tttgatattg gattttatgc tgcacgtttc
    18421 ctgatcgata cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa
    18481 ttaattattc gtgagagcgt aaaagtattg acaaagggct aactcggcgg tatattgctt
    18541 atgtaagcat ttacaaaggc tagttagtgc tttttttatt tttagtaaac cagtttacta
    18601 aagtggttta cctttcctaa aaataatgta taataccgaa tgaagacaag gaggatcagg
    18661 caatgcgaat ggtggatctg attgaaaaaa agagggatgg aaacgagctg tcgaaagagg
    18721 aaatcgagta catcgtcacg aactatacga atggaaagat tccagattat caagtcagtg
    18781 cattattgat ggcaattttt tatcaagaca tgacaaatga agaaatcacg aacttaacat
    18841 tggcgattgc taattctggt gatgtcatcg atttgagttc tcttgaagga ataaaggtag
    18901 ataagcattc gacaggtggc gttggtgaca cgaccacatt gattttagcc cccctagtag
    18961 ctagtgtcgg agtgacagtg gctaaaatgt ctggacgcgg tctaggttac acaggtggta
    19021 cactagacaa gctagaagca attcccggat ttcagatcga gctttcggat gaagaatttg
    19081 tacgcattgt taatgaaagt aaagtagcgg taattggtca atcaggaaac ttggcaccgg
    19141 cggacaaaaa attgtatgcc ttgcgtgacg tcacagcgac cgttgattct cttccgctga
    19201 ttgccagttc gattatgagt aaaaaaatcg cggcaggagc agatgcaatc gttttggatg
    19261 tgaccactgg agacggtgct ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga
    19321 ccatgacgag cattgggaaa ctggctaacc gagaaacagt ggcggttatc tcagatatgt
    19381 cagaacctct tggcgaagct atcggaaata gtctagaagt tgtcgaggca atcgaaacat
    19441 tacaaggaaa tggtccagaa gatctagtag aaatgtgcta cgccttagga agccaaatgg
    19501 ttgttcttgc tggcaaagct aagacaaccg atgaagcaag aacgttgctt caagaagctc
    19561 tggaatcagg taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa gggggagacc
    19621 cagcgatcgt ggaacatcca gaacgaattt tgactgcacg ctacacaatg gagcttcctg
    19681 ctaaacaaag cggagttgtg tcaaaaatcg tggcaaatga attaggaatt gctgcaatga
    19741 tgctaggtgc tgggcgtaaa accaaagaag aagatattga tcacgctgtt ggattgaaac
    19801 tgcataaaaa aattggtgac actgtgacaa aaggtgagtc actattgacg atctatagta
    19861 acgacgaaga aatcacttct gtcattgaat tattatataa aaatattgaa attggtgaat
    19921 cggcaatgaa gccaacgttg attcatgaca tcatcacaga ataaaggaaa aaagcggact
    19981 ttaccgtttt aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta
    20041 ttttgaaagc agacgcatct aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat
    20101 accattttta ttctgtctgc atcaatccag catgggttgc gctagcgaaa gaacaattac
    20161 aaggtgagcc agtagccgta tgtacagtaa tcgggtttcc tctaggagca aacacacccg
    20221 agacaaaagc atttgaaaca acagatgcca tcaataacgg tgcagatgaa gtcgatatgg
    20281 tcatcaatat cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag
    20341 cagttgttga agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat
    20401 tagatagaga agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc gctgattttg
    20461 tcaaaacatc aactggtttc tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc
    20521 gtgaaactgt aggaccagaa atgggtgtca aagcttccgg cggtatccat aacgaagaag
    20581 aagcaatggc aatgatcgaa gctggtgcta caagaatcgg aacaagcgca ggagtagcaa
    20641 tcgtatctgg ttcaactggt gaaggctatt aattttttca aaggagaagc tcatggcaga
    20701 actcaaacaa gaatggatca acacagctat tgaagcgtta gataaagcct atgttcctta
    20761 ttcccatttt ccggtaggcg cttgtctcgt aacggaatca ggcaagattt atcaaggcat
    20821 caacattgag aatgcatcat ttggattaac gaattgtgcc gagcgcacag cttttttcaa
    20881 agcagtatct gaaggagaaa aaacatttac gcatctagtc gtagcggggc acacccccga
    20941 tcctatctct ccttgtggtg catgtcgaca agtaatggca gaattctgtt cccctgatat
    21001 gccggttacg ctagtaggag acaatggtgt gacgaaggca acgaccgttc gtgcactgct
    21061 gccttatgca tttactgaaa aagatctata actcatgttc attcagtcag ctgactgttg
    21121 aaaaataaaa caattatttt aggaggcttt acagaaatga aaaaagcaaa attatttggt
    21181 ttaggtgctg tcgcactagc agcagggtta tttttaggtg catgcggcaa caacggctct
    21241 actgattcat ccggtggcaa aagctcatca gacacaacaa ctgcagcatt gatcacagac
    21301 acaggtggtg tagacgaccg ttcattcaac cagtcagctt gggaaggact agaaaaatgg
    21361 gggaaagatc aaggactttc acgaggaaat gatggcttcc aatatttcca atcttcaaat
    21421 gaatcagatt acatcccaaa tattgaccaa gcattgaatg ctggattcaa aacgattttt
    21481 ggtattggtt ataaactaaa accagcgatc caagaacaag caacaaacaa tacaggtacg
    21541 aactttgtta ttatcgatga tgtcatcgat ggattagata acgttgtatc tgcaactttc
    21601 aaagacaacg aagcttctta tcttgcaggt gttgcagcag cttatacgac tgaaacaaac
    21661 gtagtcggct ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga tgctggattc
    21721 aaagccggtg ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa
    21781 tatgctggtg acttctctgc tccagataaa ggacgttcaa ttgctcaagg tatgtatgca
    21841 caaaacgcag atatcatctt ccatgcttca ggcggtacag gtaacggtgt attccaagaa
    21901 gcaaaatcat tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac
    21961 caatcagacg aaggcgaata cacattaaac ggcgaaaaga aaaacttcac attgacatct
    22021 acactgaaag cagttggaac agtagtagaa gatttggcac aaaaatcagc ggacggtaaa
    22081 ttccctggcg gcgaacacac tgtttatgga ttgaaagaag acggtgttgg tctaacagaa
    22141 ggacaacttt ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa aatcatttca
    22201 ggtgacgtaa aagttccaga aactcctgaa gaaaactaaa accatttcta tagccttgtt
    22261 agacacatgt ttgacaaggc tatggtttga ttagaacaag aattgttcat ttatccaagc
    22321 tgccttgtga cagtttggat aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa
    22381 gaacgctcac taagcgtgaa tcttagtcaa tcgttgccct agatagaaat cgttaggatg
    22441 tgaaaaaaag gtggttcaag aaaatattgt catagaaatg cagaatatta ccaaagaatt
    22501 tggaacattc aaggcaaatg acaacatcaa tttacaagta aaggcaggag aaattcatgc
    22561 gttgcttgga gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt ccggattatt
    22621 agagccgaca tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca caagtccgac
    22681 caaagccaac caattaggga ttgggatggt ccatcagcac tttatgcttg ttgatgcctt
    22741 tactgtaaca gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga tgcttgacca
    22801 taaaaaagca cgaaaagaga tccaaaaagt ttctgaacag tatggattgt cagtcaaccc
    22861 agatgcttac gttcgtgata tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac
    22921 actttaccga ggagcagatg tactgatttt tgatgagccg acagctgtat tgacccctca
    22981 agaaattgat gaattaatcg tgatcatgaa ggaattagtc aaagaaggca agtcgatcat
    23041 tttgattacg cataagttag atgaaatcaa agcagtagct gaccgctgta cagttatccg
    23101 ccgtggaaaa ggaatcggta cagtcaacgt taaagacgta acctcacaac aattagccga
    23161 tatgatggtt ggaagaacgg tttcattcaa aacgatgaaa aaagaagcga agcctcaaga
    23221 agtcgttttg tctattgaaa atctagtggt gaaagaaaat cgtggattag aagccgtgaa
    23281 aaacttgaac ttagaggttc gtgctggcga agtacttggt atcgctggaa tcgatggaaa
    23341 cgggcagtcg gagttgatcc aagctttgac tggtttgcga aaggcagaaa gcggacatat
    23401 caagctaaaa ggggaagaca tcactaataa aaaaccccga aaaatcactg aacatggtgt
    23461 aggacacgtg ccagaagacc gtcataaata cgggttagtc ctagatatga cattatctga
    23521 aaatatcgca ctacaaacct atcatcaaaa accttacagt aaaaacggca tgctgaatta
    23581 ttcagtaatg aatgaacatg ccagagaatt gatcgaagaa tatgatgttc gaacaacgaa
    23641 tgaacttgtt cccgcaaaag ctttatcagg cggaaatcag caaaaagcaa ttatcgctcg
    23701 tgaagtcgac cgagatcctg atctgttgat tgttgcaaat ccaactcgtg gactggatgt
    23761 aggtgcgatc gaatttattc ataaacgtct gatcgaacaa agggacaaat acaaagcagt
    23821 gttactgatc agtttcgaat tagaagaaat tttaaatgtt tcggatcgta ttgctgttat
    23881 ccatgaagga gaaattgtcg gtatcgttga tccgaaagaa acatctgaaa atgaattagg
    23941 tctattgatg gcgggctata ccttagaaga agcacgaaaa gagttagaaa agggggctgg
    24001 cgcacatgaa tgatcgcagc gaaaaattcc gtaatttatt agtgcctgta ctttcagttg
    24061 ttttaggatt gatcttaggc gcgatattga tggctgcttt tggttttcag cccatccaag
    24121 gttattcttc tatgctagat gcagcccttg gttcgcaaag agggataggt gaaacgttaa
    24181 ggcaagcaac gccgttgatt tttacagcat taggtttttc tgtagctaac tcagctgggt
    24241 ttttcaatat tgggctttct ggacaagcac tttgcgggtg gatcgtcagt gtctgggtgg
    24301 cacttgcgtt tccagatctg ccaaaagttg ttttgcttcc gttatgcgtg atcttaggtg
    24361 ccttagctgg tgcgatggca gcggctgttc caggtttact tcgagcattt tttggaacaa
    24421 gtgaagtgat cgtaacaatc atgatgaact atatcttatt atacgtaagc acatttgctt
    24481 tacatgaagt gatgcctgaa agctatcgct caagtttgga ctcttccaat atgatcagtc
    24541 aaaatgcctc actgcggtta ccatggttaa cacaagcttt tggcggctct cgcgtaaact
    24601 caggattatt tttagcaatc atcgcgctta ttttcgtttg gctgctgatg aagaaaacga
    24661 ctttaggatt tgaaatccgt tctgttggat tgaatccttt tgcttctgag tatgcaggaa
    24721 tgagtagtaa acgtacgatc gttttgtcga tggtcatttc tggcgctttg gcagggcttg
    24781 gcggtgtggt tgaaggatta ggaacgtatc aaaacttctt tgttcaaacg acttcgctat
    24841 ctattggatt tgacggaatg gctgtttcct tgcttggttc tggatcagca atcggtattc
    24901 ttttatcagc gatcttgttc agtattttga agatcggtgg acttggcatg cagacaggag
    24961 caggtgttcc gtttgagatc gtcaatgtat cgattgcttt gattatcttc tttgtcggga
    25021 tcaacttcct gattcgtttc gtcatggcta aagtcttcca agggaaaaaa caagaagaga
    25081 tcgttgcgac aatagaatcg aaaccaacga atcaaaacca tgaagggggc gaactataat
    25141 gtcgactatc gaattaattg ccttgatcct tacttctaca ctggtttatt caacaccgct
    25201 aattctaacg tcactaggcg gaactttttc tgaacgaagc gggatcgtaa acgtagggct
    25261 agagggaatc atggtcatgg gtgcttttag ttccatcgtt ttcaacttga cgatgagtcc
    25321 atcacttggt tctatgacac catggatcgc tattttagtg ggtggcgtcg taggtatcct
    25381 attttctctg attcacgcag tagcaacagt cagtttgcgt gctgaccata ttatcagcgg
    25441 gacagtaatt aatctgatgg cacctgcttt aggtgttttc ttgatcaaag catggtatgg
    25501 taaaggtcaa actgataaca tcacgcagaa tctggggtat ttttctttcc caggattagc
    25561 agatattccg gtcattggac caattttctt taaaaataca ttcttacctg cttattgtgc
    25621 tattttgatt gctgtcctag cttgggtgat catcttcaag acgaaattcg gcttgcgcct
    25681 ccgttcagtt ggtgaaaatc ctcaagctgc cgacacatta ggtatcaatg tatatgctat
    25741 gcgttattca ggcgtactga tttctggctt gttaggcggt atgggcggtg ctgtttttgc
    25801 tcagtcgatt tccggtaact tttcagctgg tacgatcgtt ggacaagggt tcatctccat
    25861 ggctgctatg atctttggta agtggaatcc tttaggggca atgggtgcct cattgttctt
    25921 tggttttgct caaagcttga gtatcattgg cgcacaatta ccagttattt cttcgattcc
    25981 gccagtattg ctgcaaattg ctccatattt gctgacaatc attgtgctgg ttgttttctt
    26041 agggaaagct tctggtccaa aagcaaacgg gaagaactat atcaaatcaa aataatcttt
    26101 tagcgaataa aaagggtggg ttacgggaag tgtttttcgt ctcgcccttt ttgttttaaa
    26161 ggcaataaaa ataaaaagaa agaaggaata atcatgttta aacgcgtaca tctgatcgtc
    26221 atggattcag taggaattgg agaagcacca gatgccgata agttcggtga tgttggttct
    26281 gatacattag gacacattgc gaaagaagca ggattgaacg ttccacattt agagcagctt
    26341 ggtttaggaa cgatccgtcc tcttgaaggt gtggaatcag tgaaagatca tcaaggctat
    26401 gcgacgaaat tagaagaagt ttctgtaggg aaagatacga tgacaggcca ctgggaaatc
    26461 atgggattga atatcaaaac tccatttcgt gtgtttccta atggattccc tgatgatctg
    26521 atcaaacaaa tcgaagaatt ttctggccgt aaagtcgtat gtaacaaacc ttatagcgga
    26581 acagctgtta ttgacgatta cggtgaacac caaatgaaaa caggcgattt aattgtatat
    26641 acttctgccg atcctgtcct tcagatcgcg gcacatgaag aaatcattcc tttagaagaa
    26701 ttatatcgga tttgccaatt cgttcgtgat ataacaaaag atgatccgta tatgattggc
    26761 cgaatcattg cacgtccgta cgtgggtgaa ccaggagact tcacacgaac aagcaatcgc
    26821 catgattatg cgttgaatcc attcggacac acagtccttg attctttgaa agaagcagga
    26881 aaggacgtga ttgctgttgg aaagatcaac gatattttca acggacaagg aatcactgaa
    26941 tctgtccgca cgaaaagcaa tatggatggc gttgaccagc tgctgaaagt tatgaaaaac
    27001 gatttttcag gaatcagctt taccaattta gtagattttg atgcattata cggtcatcgt
    27061 cgggatgtag tcggctatgc gcatgcgatt gaagaatttg acttacgaat tccagaattg
    27121 cttgaagcga tggaagaaga cgacttgcta atgattacag ccgatcacgg aaacgatccg
    27181 acgtttactg gtaccgatca tacgcgagaa tatgtaccgt tattggttta cagtaaaaaa
    27241 atgaaagaac aaggggcatt gcctcaagga tattattcag atatttcagc aacgattgct
    27301 gagaacttta gtgtttcttc gactgaaaat ggcgaaagct tcttgaaatt attaaaataa
    27361 tctttttaaa taggagaaag caaatgacca agttaagcga gatgttacaa gaaacgaccc
    27421 agtttatcct tgataaagga gtaaaagaaa ttgattttgg tttgattcta ggttcaggat
    27481 taggtgaatt agcagatgaa atcgaagatg ctgtaatgat ttcttatgat gagatcccgt
    27541 tctttcctgt ttcgacagta gttgggcatg caggaaagct agtttatggg tctctggcag
    27601 gcaagaaagt gttagccatg caagggagat tccactacta tgaaggtcat tccatgcaaa
    27661 cagtgaccta tccagtacgt gtgatggccg gactaggggc ccattcagtc attgtgacta
    27721 atgcatgtgg tggtgtcaac gaatcatttg taccaggaga tttgatgctg attaccgatc
    27781 atatcaattt tactggtgaa aatcctttga tcggtccgaa tgaagatgca atgggcccta
    27841 gatttccaga tatgagtcaa gcttatacgc ctgactatca agcaacagca aaagcagcag
    27901 cagaaaaact aggtttggat cttaaagaag gcgtatatat gggctattct ggaccaactt
    27961 atgaaacgcc tgcggagatc cgaatgtctc ggacaatagg agccgatgca gtagggatgt
    28021 cgactgttcc agaagtcatc gtagcagctc acagcggatt aaaagtgtta ggaatctctt
    28081 gtattaccaa cttagcagca ggtatgcaag cgaatctgag ccatgaagaa gttgtagaaa
    28141 caactcagag agtcaaacaa tcttttaaag cattggttaa agaaacattg acattattgt
    28201 aataggagga acaataaaat gagtgtgcat attgaagcga aagaaggcga gatcgccgat
    28261 aagattttac ttccgggaga tcctttgcga gctaaatata ttgcggagac ttttttggaa
    28321 gatccagttt gttacaatca ggtacgcggt atgttaggct ttaccggcaa atataaagga
    28381 gaacgtattt ctgttcaagg aacaggaatg ggaatgccat cagccaccat ctatgcccat
    28441 gaattgatcc agtcgtatgg tgtaaagaaa ttgattcgag taggtacatg cggtgcattg
    28501 tcaaaagacg tccatgttcg tgatttagta ctagcacaag gtgcagcgac cagttcttcg
    28561 atgatcgaaa aaaatttcca agcctttcat tttcctccaa tcagtgattt caatctatta
    28621 ctgaaagcat atgagatagc taaagaaaag ggctatacgg ttcatgtagg aaatgttctt
    28681 tcggaagatt ctttctacaa agatgatttg acagagacgt tccaattagc tgaacttggc
    28741 gttttaggag tggaaatgga agcagcggct ctttattact taggcgcgaa ataccatgtg
    28801 cagacattaa gtttgatgac cgttagcgac catttgatca ccggtgaaga aacaacagct
    28861 gcagaacgtc aatctacgtt caatgaaatg atcgaagtag gattggaaac agcgatagca
    28921 gaataagaag gaaatgaaaa agctgccctg cattaagtaa taagaacatc caagcaaaaa
    28981 tagctcctca tgtttttgac ttggctggac ttattattgc aaatgtaagc ttttgaacac
    29041 cgtttaccaa gaggttgtga atctgccgtt taactctgag tcacaaggaa caatttcaaa
    29101 aaacagcttt tcatgtttga tgaatattgt gacttgtgac cgagaagttg gcaggagaaa
    29161 accgtttata cgaaaaaagg gactgtgaca aatcgtgtca cagtcccttt tttatagtgt
    29221 tttttatagg tagtattttt tagcaactgt taagtcatca tttaattcat aaacaagtgg
    29281 ttgacctgtt ggaatttcaa gatccatgat gtcttcgtca gagatacctt cgatatgttt
    29341 agctagggcg cgtaatgagt taccatgtgc agctactaaa acagttttgt tgtctagtaa
    29401 tgctggagca atttcatctt gccagaatgg caaagcgcgt tctaatgtca ctttcaagtt
    29461 ttctccacca ggaatatcgc gtttgtctaa cattgcgtaa cgacggtcat ttgctgcaga
    29521 accttcgtca gtttcttcca ttaatggagg aagagtatcg taagaacgac gccagatgtg
    29581 cacttgttcg tcgccgtatt tttcagctgt ttcttttttg ttcaaacctt gtaatttacc
    29641 atagtgacgt tcgtttaagc gccatgattt gatttgtggt acccaaagtt gatctgaata
    29701 ttctaagatc aagttacatg ttttgattgc acgagttaaa acagaagtgt aagcaacatc
    29761 aaattcgatt ccagcttctt tgattttgcg tccgccttct tttgcttctt cgattccttc
    29821 tggtgctaga tttacatcag cccaaccagt aaattggttc aatgcattcc attcactaag
    29881 accgtgacga gaaaaaacta attttggcat gtttagctct ccttttaaga taggtttata
    29941 tttctgaaat tttcagttct gttttattgt acaatttttt ggaagagatt tccatcgaaa
    30001 aattagataa gattctatga gaaaacaagt agagaatata aaaaaacgaa cagtgcccac
    30061 gcaactgttc gtttttaggt attagatgca taattgctcg atttcttttt caggtaaatt
    30121 gatatattta taacatgttc caactgaaac accgcatttt gaagaaataa attggatcgt
    30181 ttcttttttt tcataataga gacgtcggat ttttttgatc gtttttgcat cgatttttgg
    30241 tcttccgccg atttttcctt ttttacgagc ttcatctaat ccaactaatg tacgttcttt
    30301 gatcaatgcg cactccatgt ttgctaggtt ttccattaat tggaaataga tttttcccat
    30361 ttcgcgcgta tcgattcctt catcaagact aatcaagtgc agcccgcgat ctttgaataa
    30421 ttgcgtgagt tctgttaatt gccgcgtaga tttgcctaat cgatgaagtt ggcaaatgac
    30481 tagtgcatcg ccttgttgca tgtcagcgat cactgactcc aaatgaacag cagtagaatc
    30541 attttgttcc acttcatatg tctcatggaa aatttcttga caaccaaagt cagataattg
    30601 atcaacctga gttgttaaat caacgtcatt tatggttgtg cgtgcataac caataatttt
    30661 catggtgatt cctttctagc agtgattttc taactggata taacagtaaa aatgtagcat
    30721 aaagtacgtc aaaaagatag ttttttcgca aattttccgc tgtctttttt tattggatga
    30781 tcaagcagtt tttctttatt tacgacggat tgcgtcaaga atctcaacgc ctttttctga
    30841 gccgacaact actttagaaa ccatgtttag gaaaagccca tgttctacta ccccaacaag
    30901 ttcatctaaa taggtagcta agctttctgg ctgttcgatg acttctagat gcagatcaat
    30961 gatgtaatga ccaccatctg tggttaagac ttctccgttt tcgtctgtac gcaagacggg
    31021 ttggaatctt ttttcttcaa aaatatgaag tagctgttgg gagccataag ggatcacttc
    31081 aactgggagt gggaacttcc caagtttatc gactaattta gaagaatcaa cgatccaaat
    31141 cgtttctttt gagtacgtag cgactatttt ctcgaaaagc aaagcagcgc cgccgccttt
    31201 gatcccttga aaatcagcac tgatttcatc agcaccgtct attgtcaaat cgactacggg
    31261 gacttcatca atactcttta acggtattcc taatttttcc gcttgttctt ttgtcgcatt
    31321 tgaagtagta actcctgtga tacgtaaacc ttcttcacgc atacggcgac caatctcttc
    31381 taccatataa taggcggttg aaccggtccc gagacctacg atcatcccgt cctttacgaa
    31441 tttcgcggct tcaatgccag ctaattgttt taaattcatc tttgcgtcca ccttttttta
    31501 gtttgagcat cgtgctttcc tcactatttt agcgtgattt acggtaaaaa gataccattc
    31561 ttttttattc caataaagct ttttcaaaaa aaattgcatc tcttgttgac atgttttttc
    31621 attcgtgata ttctaacaaa ggtgctttat ctacaggttt ctgtgaagaa acgtgctgac
    31681 tgtttatcaa agcatatttg ataaggatgc agacattgca tttatttttt atcacaaaaa
    31741 aagaaccacc tggatgtgtg gaactagacg caaatcgtgg gaaggaggaa acaaggaatg
    31801 cctacaatta accaattagt acgtaaacct cgtaaatcaa aggtggaaaa atctaattca
    31861 cctgctttga acaaaggata taacagcttt aagaaaactc aaactaacgt aaactcacct
    31921 caaaaacgtg gggtagcaac tcgtgtgggt acaatgacac ctaaaaaacc gaactcagct
    31981 ttacgtaaat atgcacgtgt tcgtttgtca aacttaattg aagttactgc ttatatccca
    32041 gggattggtc ataacttaca agaacacagt gttgtattat tacgcggtgg tcgtgtaaaa
    32101 gacttaccag gggtacgtta ccatatcgtt cgcggtgctt tagatactgc tggtgttaac
    32161 gaccgtaaac aaagccgttc taaatacgga actaaaaaac ctaaagctta attttaatta
    32221 ttaaacaaat gaaacttgct tctactatat agaagtaatt actatgaaca atatttcgga
    32281 aggaggagtt acggatgcca cgtaaaggtc ctgttgcaaa acgtgatgtt ttaccagatc
    32341 ctatttataa ctcaaaatta gtaactcgct tgatcaaccg tgtaatggtt gacggaaaac
    32401 gcgggattgc agctaacatt atctataatg cctttgactt gatcaaagaa tctacaggta
    32461 acgatccatt agaagtgttt gaacaagcaa tgaaaaacgt tcaacctgtt cttgaagtta
    32521 aagcacgtcg tgttgggggt tcaaactacc aagtaccagt tgaagttcgc cctgaacgcc
    32581 gcgttactct aggtcttcgt tgggtagtaa actacgctcg cttgcgcggt gaacacacaa
    32641 tggaagaacg cttagcaaaa gaaatcatgg atgctgcaaa caacacaggt gcttctgtta
    32701 aaaaacgtga agacactcat aaaatggctg atgccaaccg tgcgtttgct cactatcgct
    32761 ggtaagattc tctctgtttg gtcggctttt ataagtcgac ctcagatagt gtccttattt
    32821 ttagcaacta gatagtaaag agaggagaaa caaggagaat gaccagagaa ttttctttac
    32881 aaaacactcg taatatcggt atcatggccc acatcgatgc tggtaaaacg actgctactg
    32941 aacgtatctt gtactatact ggtaaaatcc ataaaattgg tgaaactcat gaaggagctt
    33001 cacaaatgga ctggatggaa caagaacaag aacgtggtat tacgatcact tctgctgcta
    33061 ctacagcaca atggaagggt caccgaatca acatcatcga cacaccagga cacgtagact
    33121 tcacagttga agttgaacgt tcattgcgtg tacttgatgg tgctgtaact gttcttgatg
    33181 cacaatcagg tgttgaacct caaactgaaa cagtttggcg tcaagcaaca acttatggtg
    33241 tacctcgtat cgtatttgca aacaaaatgg acaagatcgg tgctgacttc ttatattcag
    33301 tatcaacttt acatgaccgt ttgcaagcaa acgctcaccc aatccaatta ccaattggtg
    33361 cggaagacga cttcacagga attatcgacc ttgtaacaat gaaggctgaa atgtatacaa
    33421 acgatcttgg aacagagatc gaagaaacag aaattcctga agaataccgc gaattagctg
    33481 aagaatggcg cgaaaaattg gttgaagctg tagctgaaac tgatgaagaa cttactttga
    33541 aatacttaga aggtgaagaa atcactgaag ctgagttaaa agaaggtatc cgccgcgcaa
    33601 cagttaacgt agaattctac ccagtactat gtggttcagc atttaagaac aaaggggttc
    33661 aattactatt ggacgctgtt cttgattact tgccatcacc gttagatatc ccagctatca
    33721 aagggattga ccctaaaact gatgaagaag tagaacgtcc tgcagacgac agcgctcctt
    33781 tctcagcttt agcatttaaa gttatgactg acccattcgt aggtcgttta actttcttcc
    33841 gtgtgtactc tggtgtttta caatcaggtt catacgtaca aaacgcaaca aaaggcaaac
    33901 gtgaacgtgt tggtcgtatc ctacagatgc atgccaactc tcgttcagaa atctctgaag
    33961 tttacgctgg agatatcgct gctgctgttg gtttgaaaga cacaactact ggggacacac
    34021 tatgtgatga aaagagtcta gttattcttg aatccatgga attcccagaa ccagttatcc
    34081 aagtcgctat tgaacctaaa tcaaaagctg accaagataa aatgggtgtg gctttacaaa
    34141 aactatctga agaagatcct actttccgtg ctgaaacaaa cgtggaaaca ggcgaaacaa
    34201 tcatcgctgg tatgggtgaa cttcacttag atatcatcgt tgaccgtatg cgtcgtgaat
    34261 tcaaagttga agctaacgta ggtgcgcctc aagtatctta ccgtgaaact ttccgtgctg
    34321 gcactcaagc cgaaggtaag tttgtacgtc agtctggtgg taaaggtcaa tacggtcacg
    34381 tatggattga atttacacca aatgaagaag gtgctggttt cgaatttgaa aatgcgatcg
    34441 tcggtggggt tgttcctcgt gaatacattc cagctgttga aactggtttg aaagatgcta
    34501 tggaaaatgg tgtcttagct ggctatccat tagtagatat caaagctaaa ctttacgatg
    34561 gttcatacca tgatgtcgat tcaaatgaaa ctgccttccg tgttgctgct tctatggcat
    34621 tacgtgcagc agctaagaaa gctaatccag taatccttga accaatcatg gctgtagaag
    34681 ttgttatccc tgaagattat ttaggtgatg ttatgggtca cgtaactgct cgtcgtggac
    34741 gtgttgaagg tatggaagct cgcgctggcg gccaacaagt tgttcgtgcg atggttccac
    34801 ttgctgaaat gtttggttat gctacaactc ttcgttctgc tactcaaggt cgtggtacat
    34861 ttacaatgac atttgaccac tacgaagatg taccaaaatc agtacaagaa gaaatcatta
    34921 agaaaaacgg cggaaaagct gaataatttc ttaaaaccgg atagtcctgc aaagagatta
    34981 tccaaagaga ctatattttt aatatagttt cgtgtaaaat aattatcgat gatatgggca
    35041 tgtggtgtac cttgtgtaca tcatatacct atcagacaaa taaaaaatat attgtatttt
    35101 taggaggaca ttttaaaatg gcaaaagaaa aatttgaccg ttctaaacca cacgttaaca
    35161 ttggtactat cggacacgtt gaccatggta aaactacatt aacagctgca atcacaactg
    35221 tactatctaa gaaaaacggc ggccaagcta tggcttacga tcaaatcgat ggtgctccag
    35281 aagaacgcga acgcggaatc acaatctcaa cagctcacgt tgagtatgaa acagatacac
    35341 gccactacgc tcacgttgac tgcccaggac acgcggatta cgttaaaaac atgatcactg
    35401 gtgctgctca aatggacgga gctatcttgg tagtttctgc tgctgacggc ccaatgcctc
    35461 aaactcgtga acacatccta ttgtctcgtc aagttggtgt tccttacatc gttgtattct
    35521 tgaacaaagt agacatggtt gatgacgaag aattactaga attagttgaa atggaagttc
    35581 gtgacctatt aacagaatac gaattccctg gtgacgatgt tcctgtagtt gctggatcag
    35641 ctttgaaagc tctagaaggc gacgcttcat acgaagaaaa aattcttgaa ttaatggctg
    35701 cagttgacga atacatccca actccagaac gtgacaacga caaaccattc atgatgccag
    35761 ttgaagacgt gttctcaatt actggacgtg gtactgttgc tacaggtcgt gttgaacgtg
    35821 gacaagttcg cgttggtgac gaagttgaag ttgttggtat tgctgaagaa acttcaaaaa
    35881 caacagttac tggtgttgaa atgttccgta aattgttaga ctacgctgaa gctggagaca
    35941 acattggtgc tttactacgt ggtgttgcac gtgaagacat ccaacgtgga caagttttag
    36001 ctaaaccagg tacaatcaca cctcatacaa aattctctgc agaagtatac gtgttgacaa
    36061 aagaagaagg tggacgtcat actccattct tcactaacta ccgtccacaa ttctacttcc
    36121 gtacaactga cgtaacaggt gttgttgaat taccagaagg aactgaaatg gttatgcctg
    36181 gcgacaacgt aacaatggaa gttgaattaa tccacccaat cgctatcgaa aacggtacta
    36241 aattctcaat ccgtgaaggc ggacgtacag ttggtgccgg tgtcgttaca gaaatcaaag
    36301 cttaattcac ttaagtttta aagtaaagca acatttattc ggacgtaatc gctcggaacg
    36361 aaggtcttag gatctttgtt ccgagctttt ttatttattg caggatcagg aaaaaagaca
    36421 ggagtattat aaatagaaga aacacgaaat aaaagagtca gtattttcta aatgaaactt
    36481 tatgaaaaca aaatgttttt tacaagaaaa agccttgctt tgtgagttta tatatattat
    36541 aatgacaaag gtgcgtttac tttagtgaat tgcacagacg taatcagaat gatttcgtcg
    36601 gctaagaaac gagaggttgc gacacgcccg gacgctttgc cacgaacgag cgtgacggaa
    36661 attttcgtgg agctatgtct acttttcaaa ataggcgaag gagggaacaa gatggcaaaa
    36721 caaaaaattc gtatccgttt aaaagcgtat gaacaccgta ttttagatca atcagcggat
    36781 aaaatcgtgg aaactgcaaa aagaactgga gctagcgtgt ctggtccaat tccattacca
    36841 actgaacgca gtctttacac aatcatccgt gcgactcata aatacaaaga ttcacgcgaa
    36901 caattcgaaa tgcgtacaca caaacgtctt atcgacattg tgaacccaac accaaaaact
    36961 gttgatgctt tgatgaagct agacttacca tctggtgtaa acatcgaaat caaattataa
    37021 aactaaaaca aaaataatgg aggtgtactc atgaccaaag gaatcttagg gaaaaaagtg
    37081 ggaatgacac aaatcttcac tgaatctggt gaattaattc cagtaactgt tgtagaagct
    37141 actccaaacg tagtactaca agtgaaaaca atggaaacgg atggctacga agctattcaa
    37201 gttggttacc aagatatgcg tgaagtttta tcaaacaaac ctgcgaaagg tcatgttgca
    37261 aaagcaaaca cggctcctaa gcgcttcatt cgtgaattca aaaatgttga gctaggagaa
    37321 tatgaagtag gaaaagaaat tacagtagat gttttccaag caggagacat tattgatgta
    37381 acaggtacta cgaaaggtaa aggattccaa ggggttatca aacgccacgg acaaagccgc
    37441 ggaccaatgg ctcacggttc tcgttaccat cgtcgtcctg ggtcaatggg tccagttgca
    37501 cctaaccgtg tatttaaaaa caaacgtctt gctggccgta tgggtggcaa ccgcgtaact
    37561 atccaaaact tggaagttgt acgtgtcgat gcagaaagaa atgttatctt gatcaaaggt
    37621 aatgttcctg gagctaaaaa atctttaatt actatcaaat cagctgtgaa agctaaataa
    37681 ttgtaggaag aaaggaggaa ctaaggaatg ccgaatgtag cattattcaa acaagatgga
    37741 agccaaaacg gtgaaatcac acttaacgaa gaaatcttcg gaattgaacc taatgaaagt
    37801 gttgtttacg atgcaatcgt aatgcaacgt gcgtcattaa gacaaggaac acacgcggtt
    37861 aaaaaccgta gcgctgttcg cggcggcgga cgtaaaccat ggcgtcaaaa aggaacaggt
    37921 cgtgcacgtc aaggatctat ccgctcacca caatggcgtg gaggtggcgt agtctttgga
    37981 ccaacaccac gttcttacag ctacaaactt cctaaaaaag ttcgtcgttt agcaatgaaa
    38041 tctgtattgt cagaaaaagt tgctgaaaac aacttggtag caatcgaagg cttgaacttc
    38101 gacgcaccaa aaacaaaaga attcaaacaa gttcttgcta acttatctat cgactctaaa
    38161 gtattggttg ttctagaaaa aggtaacgac tttgctgctt tatcagcacg taacttacca
    38221 aacgtttctg tagtaacttc tgataatgtg agtgtgctag acgttgtatc aaatacaaaa
    38281 gtgttagcaa cacaaactgc tcttactcaa attgaggagg tgcttgcata atgaacttac
    38341 tagacgtaat caaacgccca gtgatcactg aaaaatcaat gcttgccatg gatgacaaga
    38401 aatacacttt tgaagtggac actcgcgcaa acaaaacttt agtaaaacaa gcggtagaag
    38461 ctgcttttga cgttaaagtc aaaaacgtga acatcgtgaa cgtacgtgcg aaattcaaac
    38521 gtatgggcaa atatgcagga tatacaaaaa aacgtcgcaa agcgatcgtt acattgactg
    38581 aagattcaaa agaaatccaa ttattcgaag ctgctgaata agcactaata gataactcat
    38641 taaattagga gggaaaaacg tggcgattaa aaagtacaaa cctacctcaa atggtcgtcg
    38701 taacatgaca ggttctgatt ttgctgaaat cacaacatca acacctgaaa aatcattgtt
    38761 acaaccattg aaaaaccatg ctggacgtaa caacaacggc cgcatcactg tacgtcatca
    38821 aggtggcgga cacaaacgcc aatatcgcgt gatcgacttc aaacgtaaca aagataacgt
    38881 tgttgcaact gttcaaacga tcgaatatga tccaaaccgt tcagctaaca tcgcgttagt
    38941 acactatgaa gatggagtga aagcttacat cttagcacct aaaggactag aagtgggtat
    39001 gaaacttgtt tcaggtccag aagcagatat caaagtcgga aatgctcttc cattagaaaa
    39061 cattccagtt ggtactgtta tccacaacat tgaaatgaaa cctggtaaag gtggacaatt
    39121 gatccgttct gctggtacaa gtgctcaagt acttggtaaa gaaggcaaat acgtattgat
    39181 ccgcttgaac tctggtgaag ttcgtatgat cttagctgca tgccgtgcta caatcggttc
    39241 tgtaggtaac gaacaacacg aattaatcaa catcggtaaa gctggacgct ctcgttggat
    39301 gcgtaaacgc ccaactgtac gtggtagcgt aatgaaccct aatgatcacc cacacggtgg
    39361 tggtgaaggt aaagctccta tcggacgtaa agcaccagtt tctccttggg gtcaaccagc
    39421 tcttggttac aaaactcgta acaaaaaagc taaatcagac aaacttattg ttcgtcgtcg
    39481 caaaaataaa taattacttt gaccatgtgt cgcacatttt gagaggaggt tcaccatggg
    39541 tcgtagttta aagaaaggac cttttgtcga tgagcattta atgaaaaaag ttgaagctca
    39601 agcaggggtt gaaaagaaaa aagttatcaa aacttggtca cgccgttcaa cagttttccc
    39661 aagttttgtt ggatatacca tcgcagttta tgatggacgt aaacatgtac cagtttacat
    39721 ccaagaagac atggtaggac acaaattagg tgaatttgca cctacaagaa cttatcgtgg
    39781 acatgctgct gacgataaga aaacgaaacg ctaatttgag gggaggataa atccaaatgg
    39841 cagaacaaat cacatcagct aaagcaactg caaaaacagt tcgcacttca cctcgaaaag
    39901 cccgtttagt aattgacctt atcagaggta aaagtgtcgc agatgcaatt tcaatcttga
    39961 aattcacacc aaacaaatct gctggaatca ttgaaaaagt attgatgtca gcagttgcga
    40021 atgcagaaaa taactttgac ttagatgttg aaaacttggt agtaagcgaa gcttttgtta
    40081 acgaaggacc tacaatgaaa cgtttccgtc ctcgtgcaaa aggatcagct tctccaatca
    40141 acaaacgtac aagtcatatc acggtagtcg tatcagaaaa ataaggaggg ataatcagtg
    40201 ggtcaaaaag tacatccaat tggaatgcgt gtaggcatca tccgcgactg ggatgctaaa
    40261 tggtatgctg aaaaagagta tgctgagttc ttacacgaag atttaagaat ccgtaaattt
    40321 atttcaacta aacttgctga tgctgctgta tcaacagttg aaatcgaacg cgcaacaggt
    40381 cgcgtaaaca tttcaattca cacagctaaa ccaggtatgg ttattggtaa aggcggttct
    40441 gaagtcgaaa gcctaagaaa agaattaaac aaattaactg gtaaaagaat ccatatcaat
    40501 atcgtggaaa tcaaaaaacc agatctagat gcaaaattag taggtgaagg aatcgcacgt
    40561 caattagaaa accgtgttgc tttccgtcgc gctcaaaaac aagccatcca acgctcaatg
    40621 cgtgctggcg ctcaagggat caaaactcaa gtatctggtc gtctaaacgg agcagatatc
    40681 gctcgttcag aaggatactc agaaggaact gttcctcttc atactttacg tgcggacatt
    40741 gattacgcat gggaagaagc agatacaaca tacggaaaac taggagttaa agtgtggatc
    40801 tatcgtggag aaattcttcc aactaaaaaa aacactgaga aaggagggaa ataatcatgt
    40861 tagtacctaa acgtgtaaaa caccgtcgtg aattccgcgg aaaaatgcgc ggggaagcta
    40921 aaggcggaaa agaagtagca ttcggtgaat acggtttgca agctgttgat tcacattgga
    40981 tcacaaaccg ccaaatcgaa gctgctcgta tcgcaatgac tcgttacatg aaacgtggtg
    41041 ggaaagtatg gattaaaatt ttccctcaca aatcttatac tgccaaagca attggggtac
    41101 gtatgggttc tggtaaaggg gcacctgaag gatgggttgc accagtaaaa cgtggtaaaa
    41161 tcatgtttga aatcgcaggc gttcctgaag aagtagctcg tgaagcgtta cgtctagctt
    41221 ctcacaaatt accaatgaaa actaagatcg taaaacgtga ggaaatgggt ggtgaatcga
    41281 atgaaggtta aagaaatcag agaattaacc actgccgaaa tgcttgatca agaaaaacaa
    41341 ttaaaagaag aattgtttaa tcttagattc caattagcaa caggtcaatt agaaaacact
    41401 gcacgtatta aagaagtacg taaatcgatt gcacgcatca aaactgtgtt gcgtgaacaa
    41461 gctaagtaat tatggaagga ggccattaag cgtatgactg aaggaagaaa tcaacgcaaa
    41521 gtttatcaag gtcgcgtggt ttcagacaaa atggataaaa caattaccgt tgctgtcgaa
    41581 acaaagaaaa accaccctat ctatggcaaa cgcatgaact attctaagaa atacaaagtg
    41641 catgatgaaa acaacacagc aaaagttggc gacatcgtga gaattatgga aactcgtcca
    41701 ttatcagcta caaaacgttt ccgtttgttg gaagtagtcg aagaagcagt tattatctaa
    41761 taacacgaga ttttcctata tagattgaat gcaaaatctg aaaggaggat acacaacgtg
    41821 atccaagcag aaagtcgttt aaaagtcgct gataactcag gcgcacgtga aattttgacg
    41881 atcaaagtcc ttggtggatc tggtcgtaaa actgctaaca tcggtgatgt gatcgttgct
    41941 acggttaaac aagcaacgcc aggtggggtt gtcaaaaaag gtgacgtcgt taaagccgtt
    42001 atcgttcgaa caaaatcagg agctcgtcgt acagacggtt catatattaa atttgatgaa
    42061 aatgctgcag tgattattcg tgatgataaa agccctcgtg gaacacgtat ctttgggcca
    42121 gttgcacgtg aactacgtga aaacaacttc atgaagatcg tttcactagc accagaagtt
    42181 ttataatctt gagacactta tcaaaggagg tgcgaaacag ttatgtttgt taaaaaaggc
    42241 gataaagtta aagttatcac tggaaaagac aaaaacaaag aaggcgttgt actagcagcg
    42301 ttcccgaaaa aagataaagt tattgtcgaa ggtgtaaatg tcgttaaaaa acaccaaaaa
    42361 ccttctcaag ctgctcctca aggtggaatt gttgagatgg aagcgccaat tcatgtttct
    42421 aacgtgatgg tggttgactc tactggtgta gctggccgag ttggctacaa agaagtagac
    42481 ggcaaaaaag tccgtgtttc taaaaaaacc ggtgaagttt tagataaata aaagtttaag
    42541 gaaggagggt cttactgaat gaaccgccta aaagaaaaat atcttaaaga agtaactcca
    42601 tcattgatgg aaaaatttaa ctatagctct gttatgcaaa cacctaaagt tgaaaaaatc
    42661 gtcatcaaca tgggtgttgg tgatgcggta tcaaacgcta aaaacttaga taaagcagtt
    42721 gaagaattaa ctttgatcac tggtcaaaaa ccaatgatta caaaagctaa aaaatcaatc
    42781 gctggattcc gtttacgtga aggaatgcca attggtgcaa aagttacatt gcgcggagaa
    42841 agaatgtacg aattcttaga caaattggtt tctgtttcac taccacgtgt acgtgacttc
    42901 catggtgtaa gtaaaaaagc ctttgacggt cgtggaaact atactttagg tattaaagaa
    42961 caattgatct tcccagaagt agattatgat ttagtagata aagtacgtgg tatggacatc
    43021 gttattgtaa caacagcaaa ctctgatgaa gaatctcgtg agttgttgac acaattaggc
    43081 atgccattcc aaaaataatt aaaggaggcg aactacgtgg ctaaaaaatc aatgattgct
    43141 aaaaacaaac gtcctgcaaa attttcaaca caagcttata ctcgttgcga acgttgcgga
    43201 cgtccacatt cagtttatcg taagttccat ctttgccgta tttgcttccg cgaacttgcc
    43261 tataaaggtc aaattcccgg cgtgaagaaa gctagctggt aaaaagtaaa ttcgcataag
    43321 aaggaggtaa aagatcaatg gtcatgacag atccaattgc agattttcta actcgtatcc
    43381 gtaacgcaaa catggttaaa catgagtctt tagaagtacc tgcatcaaaa attaaacgtg
    43441 acatcgctga aatcttgaaa cgtgaaggtt tcgttcgtga tgttgaatac atcgaagatg
    43501 acaaacaagg cgtgatccgt gttttcctaa aatttggtaa aaacggcgaa cgtgttatca
    43561 caaacttaaa acgtatctct aaaccaggtt tgcgtgctta tgtcaaagct gacgaagtac
    43621 caaaagtatt gaacggttta ggtatcgcaa tcatttcgac ttctgaaggt gttgtcactg
    43681 ataaagaagc tcgtgaaaaa aatatcggcg gcgaagttat cgcttacgta tggtaatcaa
    43741 gtagaatcta caaggaggtg tctttaagtg agccgtatcg gaaataaagt cgttgttatc
    43801 cctgaaggag taacagttac tcaagacgga aacaacgtta cagttaaagg accaaaaggt
    43861 gaattgactc gtactttttc agcagatatc aaaatgaata tcgaaggaaa cgaagtaact
    43921 ttcactcgtc caaacgacag taaagaaatg aaaacaattc atggaacaac tcgtgcgaac
    43981 ttcaacaaca tggttgtagg tgtcagcgaa ggtttccaaa aagcgttaga actaatcggg
    44041 gttgggtacc gtgcacaatt acaaggatca aaacttgtat tgaacgttgg ttactcacat
    44101 ccagtagaaa tcacaccacc agctggagta actgttgaag taccagctaa cacacaagta
    44161 attgttaaag gtgcgaacaa agaagaagtt ggcgaattag ctgcaaacat tcgtggaact
    44221 cgtcctccag aaccttataa aggtaaaggt attcgttatg ttggcgaata tgtacgccgt
    44281 aaagaaggta aaactggtaa ataatcgttt agaaaaattg catagaagcc agcagcagga
    44341 aaaaggcatt tggctgaagt ttaccttcaa aaaaatacat ttttacttgt gccgcagctt
    44401 ctatttttcg aaacgtattt actgcttgaa atagtagtac aataaaaata aagaggtgac
    44461 aattgtgatt acaaaaccag ataagaacaa aacacgtcaa aagagacatc gtcgtgtacg
    44521 taacaatatc tctggtactg ctgagcgccc acgcttgaac gtttttcgtt ctaacaaaaa
    44581 catctacgct caaattattg atgacgtagc gggtgtaacg ctagcaagtg cctctacctt
    44641 ggataaagaa atttcaggtg gaacaaaaac agaagcagca caagctgtcg gtaaactagt
    44701 tgctgaacgt gcagctgaaa aaggcattaa agtagtagtc tttgaccgtg gtggatacct
    44761 ttaccatggc cgtgtgcaag ctttagctga agctgctcgc gaaaatggac tagaatttta
    44821 gaagaaggag gaataccatt catggtttat atcgatccaa aacatttgga attagaagac
    44881 cgcgttgttg caattaaccg tgtaacaaaa gttgttaaag gtggacgtcg tctacgtttt
    44941 gctgctttag ttgttgtcgg tgacaaaaac ggacacgtag ggtttggtac aggtaaagct
    45001 caagaagttc ctgaagcaat ccgtaaagca attgaagatg cgaagaaaaa cttagttgaa
    45061 gtacctatgg ttggttcaac aatcccacac gaagtaatcg gtgcttttag tggtggtaga
    45121 atcctaatga aacctgctgt agaaggttct ggggttgctg ctggtggacc agttcgtgcc
    45181 gtattagaat tagctggggt agcagatatc acttctaaat ctttaggctc aaatacacca
    45241 atcaacgttg ttcgcgcaac agttgaaggt ttgaaacaat tgaaacgtgc agaagaagtt
    45301 gcagaacttc gcggcaaatc agttgaagaa ttaatcggat aaggaggaca acaataatgg
    45361 ctgaattaaa agttacttta aaacgcagta ttatcggacg tcctcaaaac caacgcgata
    45421 cagttaaagc gttaggtctt acaaagatta acagttcagt ggttaagcct tctaatgaag
    45481 caattaaagg catgatcaat actgtttctc acctagtgga cgttgaagaa atttaattta
    45541 gaaaaatcat aactagaaca ttaaggaggt gccaaaccta tgaaacttca tgaattaaaa
    45601 cctgctgaag gttcacgcca agtacgtaac cgtgttggtc gtggtacttc atctggtaac
    45661 ggaaaaactg ccggacgcgg acaaaaagga caaaaagctc gttcaggcgg cggtgtgcgt
    45721 ttaggatttg aagggggaca aacaccattg ttccgtcgtt taccaaaacg aggattcaca
    45781 aacgtgaacc gtaaagacta tgcagtcatt aatcttgatg tattgaaccg ctttgaagac
    45841 ggagctgaag taactcctgt tgctttagtt gaagctggaa tcgtgaaaaa cgaaaaagct
    45901 ggaatcaaag tattagctaa tggggaacta aacaagaaat taactgtgaa agcagcaaaa
    45961 ttctctaaag cagctcaaga agcaattgaa gcggctggtg gatcaatcga ggtgatctaa
    46021 tgtttagact attaaaagat gcttttaaag taaaagacat tagatcgaaa atcctattta
    46081 ctgtatttgt gttgtttgta tttcgcctag gtgcacatat tactgtacca ggcgtaaatg
    46141 ctgcttcatt gcaaaacctt tcttctttgc catttttcaa catgttgaac ttggtaagtg
    46201 gtagtgcgat gcaaaacttt tccattttct caatgggggt ttcaccatac attactgctt
    46261 ctatcgtcat tcagcttctt caaatggaca tcgtgcctcg atttgttgaa tggtcgaaac
    46321 aaggggaagt tggtcgtaaa aaactgaatc aagcgactcg atatctgaca ttagtattgg
    46381 catttatcca atctgtcggt atcactgctg gattcaatca atacgctcaa ttaggtttcg
    46441 tgaataatcc taacgtagca acttacgtaa cgatcggttt gattctaaca gccggtacga
    46501 tgtttgttac ttggctagga gaacagatca cagaaaaagg tatcggaaat ggcgtatcaa
    46561 tgatcatttt tgcaggtatc atttctagat tgcctgaaag tatcagagaa atttatgaag
    46621 attactttat aaatattgat ccttcagaca tatggaaatc tgctattttt gtggccattt
    46681 tactagtagc ggtcttgttg atcgtgacat tcgtaacatt tttccaacaa gctgaacgca
    46741 agatcccgat ccaatatacg aaacgcgtag ctggcgcgcc tacaagcagc tatctgccgt
    46801 tgaaagtaaa tgcagctgga gttattccag ttatctttgc tagttcgttt attgctacgc
    46861 caaatgctgt tttacaagca ttgagcggta gattcggttc tgaaaactgg tacaaagtga
    46921 tgatggagat ctttaactat aatacagcac ctggtgctac gatttatacg attctgatcg
    46981 ttgccttcac tttcttctac gcctttgttc aagtaaaccc tgagaaatta gcggaaaact
    47041 tgcaaaagca aggaagttat ataccaagcg tacgccctgg taaggggact gaagagtatg
    47101 tctcaagatt actgatgcgt ttaagtacag ttggtgcagt cttccttggt ttagtggcat
    47161 tgcttccaat cattgctcaa atggtttggg atcttccaca atcaatcgga ttaggcggta
    47221 caagcttact gatcgttatc ggtgtagctt tagaaactgc gaaacagtta gaaggcttaa
    47281 tgctgaaacg taaatatgtt ggattcattg agtaagaaag cgtgttgagg tttcccctca
    47341 acacacgttc tcacacattt attaggaggg aaaacaatga acctcatttt aatggggctg
    47401 ccaggcgcag gtaaaggaac acaagctgaa aaaatcattg atgcttatgg tattccgcac
    47461 atctcaacag gagatatgtt ccgcgcagcg atgcaaaacg aaacagctct tggcctggaa
    47521 gcaaagtctt atatggataa aggtgcatta gttcctgatg aagtaacaaa tggaatcgtg
    47581 aaagagcgac tagctgaacc agatacagag aaaggcttct tattagatgg ctttccacgt
    47641 acgctagatc aagcaaaagc tctggacgca atgctaaaag atttgaataa aaaaattgat
    47701 gctgtcatcg atatccatgt tggtgaagaa atcttggtcg aacgtctagc cggccgattt
    47761 atctgcagca attgtggagc aacttaccat aaaattttca accctacaaa agttgaagac
    47821 acatgtgatc gttgcggtgg gcatgaattc tatcaaagag aagatgataa acctgagacg
    47881 gttaaaaatc gtctggctat caacatcaaa aatagtgaac caatcttggc ttattataaa
    47941 gaacaaggtt tgctaaacac tattgatggt gctcgtgaaa tcgatgctgt atttgcagat
    48001 gtacaaaaaa tcattgaaaa atagcacgat tgcccgtagc cgacttgcat tatgcagtta
    48061 ctcttgtcaa gttcaataaa ttatgataga atatgtcagt ccgacttcta gagaatcctc
    48121 tagatgatcc attttgagaa ttttgaggtg ggaactgcag tttccgccat tttatcgtgt
    48181 gaaaatgcga aacaagtaca aggaggtact gtgcgtggca aaagaagata tgattgaagt
    48241 cgaaggtaca gtcgtcgaaa ctttgccgaa tgcaatgttt aaagttgaac tagaaaacgg
    48301 acaccaagtt cttgctactg tttcaggtaa aattcgtatg cattacatcc gtatcttacc
    48361 tggtgacaaa gtaacggttg aattgtctcc atacgatctt aaccgtggcc gtattactta
    48421 tcgctttaaa taattgtact ccgtaaaact acaggaggta ttatcatgaa agtaagacca
    48481 tcagtaaaac caatgtgcga acattgtaaa gtaattcgtc gtaaaggacg cgttatggtg
    48541 atttgcccag caaatccaaa acataaacaa cgtcaaggat aattggaggt gctataaata
    48601 tggctcgtat tgcaggagta gatattcctc gtgataaacg tgtagtagtt tcacttactt
    48661 atatctatgg tattggtaac actactgcta aaaagatctt agctgatgct ggtgtatctg
    48721 aagatgttcg cgttcgtgaa ttaacgaatg aacaaacaga tgctatccgt gcagaagtag
    48781 ataaattaaa agttgaaggg gatcttcgtc gtgaagtgaa cttgaacatc aaacgtttga
    48841 tggaaatcgg ttcttaccgt ggaatccgtc atcgccgtgg attgcctgtt cgtggacaaa
    48901 acacgaaaaa caatgcacgt actcgtaaag gcccagctcg cgctatcgct ggcaagaaaa
    48961 aataattctt aagtgaagga ggttaaaact tcatggcagc aaaaaaagtg aatcgtaaac
    49021 gccgtgtgaa gaagaatata gaagcaggta ttgcacatat ccattctaca ttcaataaca
    49081 ctatcatcat gatcactgat actcacggaa atgcattagc gtggtcatca gctggttcat
    49141 taggtttcaa aggaagcaaa aaatcaactc cttttgccgc tcaaatggca gcagaaactg
    49201 ctacaaaagc agcaatggaa cacggattaa agactgttga agtaacagtt aaaggacctg
    49261 gttctggacg tgaagcagca attcgttcat tacaagctac aggtttagaa gtgactgcaa
    49321 ttcgtgacgt gactccagtt cctcataatg gtgcccgccc tccaaaacgc cgtcgtgttt
    49381 aatgagtgcc gctcattttg agttttatca gtaacgtcat tgaacaagac acttttcgtt
    49441 ttgaaagggg taaagataag aatgattgag ttcgaaaaac caagaatcgc aaaaattgat
    49501 gaagaaaaag attatggcaa gttcatcgtt gaacctcttg aaagagggta tgggactact
    49561 ttagggaatt ccctacgtcg tattttatta tcttctttgc caggtgctgc catcactaat
    49621 attcaaatcg atggtgtact gcatgaattc tctaccatca aaggtgttag agaagacgtc
    49681 actcaaatca tcttgaacat taaaggctta gcgctgaaaa tgtatggcca agaagaaaaa
    49741 acccttgaaa tcgatattac cggacctgct acagtaactg ctggcgatat tatcgttgat
    49801 agtgatgtag aaattcttaa caaagatatg tacatctgta gtgtctctga aggagctacg
    49861 ttccatgctc gcttaacagt gaaacctgga cgtggttatg ttcaagcaga tgaaaataaa
    49921 aaagaagata tgccaattgg cgttcttcca gtcgattcaa tctacacacc tgttcgtcgt
    49981 gtgaactatc aagttgagaa cacacgtgtt ggacaccgtg aagattttga taaattgacg
    50041 atggagattt ggacagatgg ttcaattgaa ccgttggaag caatgagttt gtctgcgaaa
    50101 atcatgactg aacatttgga tatttttgtg aacttaactg atgaagcaaa aaatgctgaa
    50161 atcatggtcg aaaaagaaga aacacaaaaa gaaaaaatgt tggaaatgac gatcgaagag
    50221 ttagacttgt ctgttcgctc ttacaattgt ttgaaacgtg caggaatcaa tactgtacaa
    50281 gaacttacaa ataaatctga gcctgaaatg atcaaagtcc gtaacttagg acgtaaatca
    50341 ttagaagaag ttaaagcaaa attgcacgac ttaggtttag gtttacgtaa agaagattaa
    50401 aaccttttac gaaggaggga aaccaacgtg agttatcgta aactaggacg cacatctagc
    50461 caacgtaaag cgatgttgcg tgatttaaca actgatttat taatcaacga acgtattgtg
    50521 actactgaag ctcgtgcgaa agaagttcgc tcaactgcag aaaaaatgat cactttaggt
    50581 aaacgcggag atttgcatgc acgtcgtcaa gctgcggctt tcgtacgtaa tgaagttgcc
    50641 agcgtgcgtg aagaaaatga ggacatcgtc attgaatcag ctttacaaaa actgttcaac
    50701 gatattgcac ctcgttacgc agaacgtcaa ggcggataca cacgcatttt gaagacagaa
    50761 ccaagacgcg gtgatggtgc accaatggtt gtcttagaac ttgtctaaaa tcacactaat
    50821 aacatcactt tatagatgat tcttttagag tgttatgatg ttggaagata tttttccgag
    50881 tctagctcag cgctgctcct caagcgaata tcgcttgggg agtaagcact catcataaag
    50941 tgttttttta taccttcaat tacctgtcat gacagatatg tttgaaggca taaaaaaaca
    51001 ttttaagcga gaaataattg aaaattttgt ctttttttga tagtgttagt aggaacttta
    51061 ggatgaagaa ggagtaatca tggagccaat tattgaatta gaaaagataa attataaata
    51121 ccagccagat gatcttcgcc ccgcattgaa agatgtctcc tttacaattg ataaaggtga
    51181 gtggatcgct attattggac acaatggttc aggaaaatcg acacttgcca aaacgatcaa
    51241 tggattgctt ctgccagaat cagggatcgt gaaagtggga aatcaaatat tggatgaaga
    51301 aaatatttgg acgatccgac agatggttgg aatggttttt caaaatccag acaatcagtt
    51361 tgttggttcg acagtagaag atgacgttgc ttttggtctt gaaaatcaag ggattcccag
    51421 agaagaaatg ctagtgcgtg tgaaagatgc acttgagaaa gttagaatgg ctgagtttgc
    51481 ttcacgcgag cctgctcgtt tatcaggagg gcaaaaacaa cgtgttgcca ttgccggcgt
    51541 ggttgcctta cgacctgata tcattatttt agatgaagca accagtatgc tagatccaga
    51601 aggtcgtgaa gaagtgatct ctacgatcaa gaagatcaaa gaagaaagtc aattgaccgt
    51661 tatatctatc actcatgata ttgatgaagc agctaatgcc aatcggatac tagtgatgag
    51721 acaaggcgaa cttgtccgtg aaggtacacc aaaagaaatt ttttctgctg gtcctgaact
    51781 gatcgactta ggtctagatc taccatttcc tgaaaaactg aagagcgcac tgaaagaacg
    51841 tggggttgat gtgcccagtg aatatatgac agaagaaagg atggtggatt ggttatggac
    51901 atccgttttg aacaagtaga cttcacttat cagccgaata caccatttga acaaagagcc
    51961 ttatttgaca tcaatatgac gatcaaagaa aacagttata ctgcattagt agggcatacc
    52021 ggaagcggga aatcaacctt acttcagcat ttgaatgcgc ttgttaagcc aacaagcggt
    52081 acagtccata ttggagaacg agatattcag ccggatacgg ataataagaa tttaaagccc
    52141 atccgaaaaa aagttggcat cgtctttcag tttccagaag cacagctatt cgaagaaacg
    52201 gtagcaaaag acattgcttt cggtcctaaa aactttggtg tcagcgaaga agaagcacta
    52261 gtcctagcaa aagaaacatt agaacaagtt gggctggatg aaagctattt ggaacgttcg
    52321 ccatttgaac tttcaggagg gcaaatgcgt agagtagcaa tcgctggtgt gcttgccatg
    52381 aggccggaag tacttgtatt ggatgaacca acggcaggac ttgatcccca gggtagaaaa
    52441 gaaatgatgg agatgttttg gcgtcttcat aaggaacagc agatcactat tgtattagtt
    52501 acgcatttaa tggatgatgt agccaatttt gcagattatg tctatgtgtt ggaaaaggga
    52561 cgaatcgtta atagcgggga gccgcaagag gtctttcaaa acattgaatg gctaaaagaa
    52621 aaacaactag gcgtacctac tgccaccgag tttgcagaag aattaatggc taaagggatg
    52681 aactttgcta ccttgccttt gacagctgaa gaattagcag acgctatcgt ccattatgct
    52741 ggaggaaatg cccatgatga ataaattgat atttggtcgt tatatgcctg gagattcctt
    52801 gatccatcga ttggatcccc gagctaaatt attagccagt ttttatttta tcggaatcat
    52861 ttttttagca aataattggg aaacgtacct tttgttggca tttttcacat tactttctgt
    52921 cttcttgtca aaagtagatt tgaaattttt tatccgaggc attcgcccat tgatctggct
    52981 aatcctcttc actgtagctt tgcaggtctt gtttacaagt ggcggagaaa cttattggag
    53041 ttggggtgtt ttccatatca ctgaatttgg tgtgatgaac ggcctcttta ttttctgtcg
    53101 attcgtgttg atcattttta tgtcgactct actcacgctt actacaccgc ctttggaatt
    53161 atcggatgcg attgagtact tgcttcgtcc attgaaagcc gtacggttcc cagtccacga
    53221 aatttcattg atgctgtcga ttgctcttcg ttttgtgcct acattgatgg acgaaacaga
    53281 aaagatcatg aacgctcagc gcgctcgcgg tgttgatttt ggtgaaggaa acctagttca
    53341 gaaaatgaaa gcagtcattc ctttgctgat tcctttattt gtcagcagct tcaaccgtgc
    53401 ggaagatctt gcgactgcta tggaagcaag aggctacaaa ggtggggaag gaagaacgaa
    53461 atatcgtgta ttgcattggc ataatagaga cacgatggtt atcattgctt ttgtattatt
    53521 aactgtgcta ctgatctttt tgagaggata aacgttcagt gggccgctat ctattctttt
    53581 cttgctgatc tgtttgcaag aaaggatgcg agcagtgtat gacaagaaat attagggtct
    53641 gtggcagact aaggtcacag gcctctttct tttatctgaa aagaaagaac gaaaaaattt
    53701 tataaaaaaa tcattattcc agataacctt ttaggaacaa aggagagaaa aacatggtac
    53761 gttacaaagc aatcattgca tacgacggaa caaattttaa tggattccaa aaacagccaa
    53821 atgggcgtac tgttcaagaa gaagtagaaa aaacattaca aaaaatggca aatggaaaag
    53881 aaatcacggt ctttggttcg ggaaggacag atgccggagt acatgcgatt gggcaagtaa
    53941 tccattttga ttatccagag gagcgtccgt tagaacgaat gcgtttcgcg ctagataccc
    54001 agtcaccaga ggatattgct gtaagacaag tagaaattgt ttcagaagac ttccacgcaa
    54061 gatatttggt caaagaaaag acgtatcaat ttcgagtgga tatagggaaa ccacgcagtc
    54121 cttttcgacg ccattatgca agttattttc cttaccctct tgatttggaa aaaattcagc
    54181 gggcgcttcc tgatttatta gggactcacg atttcacctc tttttgtgca tcaggaagct
    54241 cgatagagga taaagtacgt acgatctacg aggctaagat ggaggtaaat gaaacaaaag
    54301 acgaattgtt gtttacattt aggggaaatg gcttcttgta taagatgata cggatcttag
    54361 taggaacgct tttgaaaatt ggcaatggcc gattgcctga agatagtatc ccagatatta
    54421 ttgcgaaaaa agatcgtaat gcagcaggac ctacagcaca tcctgaagga ctttatttgt
    54481 atgaagtgaa atatgaataa gaggttgtga cagaagtggt cagcttcaag aaataagaag
    54541 gaatttacga aaattacttc tcaaaatttt gcaaaatttc ggcttatttt cgaaggagct
    54601 acttctggaa caccgtgaat cagaggttgt gaaaaagctg ccctgcatca agtaataaga
    54661 acaaccaaac aaaaatagct cgtcatattt ttcgtttggt tgggcttatt accgcagatg
    54721 caagcttttg aacaccgttt aaggaagagg ttgtgaaatc agcgttttga cctgagtatt
    54781 agagaaacaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgaag
    54841 gtcagctgat tgaacaccgt ttaagaaaga ggtcgtgagc ctgtcattta actctgagtc
    54901 acaagtaaca atttcaaaaa acagcttctc atctttgatg aatatcgtga cttgtggacg
    54961 aggcgttgat aagtgaatca tctttttgtt acctattttt tctgtaacca aaaaacgatg
    55021 gaacgttgtt ttgattgccc ttaatgagga cagtcggaac aggtttgtca tttttatatt
    55081 taaatggcac aaatttttct aattatttgt catttagcgc tgaaaatttt tgctatttat
    55141 ctaaaaaagt gataactact taagatgaag cagaatattt acgccatacg gcatacaacc
    55201 gcgaaaaata taaaaaaaag aaaacgattg aacgcgtttt tgcagtcgca aaggaaaagc
    55261 atggaatgcg ctacatcaag tatcgagact tggaaaacaa tcaggtgtat acgctgattg
    55321 tttttgctgc aatgaatctg aaaaagttgt ccacttggca gttggaacat atgtcttcat
    55381 atcttttatt atacaaaaat agcaaaagca ccagtctaca ctcttagtaa agaggaaact
    55441 gatccttttg tctacattct taaagtactt aaactaagta ctttatttta ctctttagga
    55501 ttgatacagt agcatctatc cctttattaa taatctttta tttttttaaa taaagatgtt
    55561 gtctccatct tcaggcacta aaactttttc ggaaattcct ttttctttat tgaaagactt
    55621 taattcttct cttgacagtc cccaatggtt tacagcttcc atatgaacgg caataattgt
    55681 ggaatctggt attgctttat gcaattcata taaatcgtct ttccccataa taagagattc
    55741 tccaactaaa aaatgattat ctccagcatt tgctactatg atttctggat tatactgatc
    55801 aatagtttgt ttaacagctt cataccaaac tgtatctcct gtaaagtata gtgtcttctc
    55861 agtcgaatgt ttgaaaacaa taccgcaaac attacctaca taattcaaga tttctcctct
    55921 gccatgtttg ccttttgttt taaccaattg tatagattca aaaactgtgt cttctcgtag
    55981 tacctccaca tttttaaaac catatagtcg aatttcattc gcatcatttt catcttgcac
    56041 aaataatttt atttctttag gtaggacctc tttcgctata tcgtcccaat gatctagatg
    56101 taaatgtgtt acaataacag catcaatacc gttgataata tcttcgactg gcattggcaa
    56161 atcaactaga ggattcattt gatcttttct tggtgatgga aaaggacctt tcgaaataaa
    56221 tggtggaagt gttccttttt ccgctaacat tggatcaatt aaaaattttt tacctccata
    56281 gtctaagata gctgttgcat tacgaatgtg atgtatattc ataaacgttg tctccttttt
    56341 gttgatatga aatattttta gtataattca attccataca ataaataaac cgaacaaaaa
    56401 aagtctttta ctatttttac ttaatttatg aaaggttgtt taccatgtta gaccaaacag
    56461 atataaatat cattgaggaa ctatcaaaaa atagtcgaat cacaatgaaa
//
LOCUS       DAZULG010000005        55171 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000005 DAZULG010000000
VERSION     DAZULG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.005"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..730)
                     /locus_tag="ACG9CY_000240"
     CDS             complement(65..730)
                     /locus_tag="ACG9CY_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS4936786.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(877..1482)
                     /gene="dhaL"
                     /locus_tag="ACG9CY_000241"
     CDS             complement(877..1482)
                     /gene="dhaL"
                     /locus_tag="ACG9CY_000241"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS4936787.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(1510..2520)
                     /gene="dhaK"
                     /locus_tag="ACG9CY_000242"
     CDS             complement(1510..2520)
                     /gene="dhaK"
                     /locus_tag="ACG9CY_000242"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS4936788.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(2495..2890)
                     /gene="dhaM"
                     /locus_tag="ACG9CY_000243"
     CDS             complement(2495..2890)
                     /gene="dhaM"
                     /locus_tag="ACG9CY_000243"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS4936789.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            3044..3364
                     /locus_tag="ACG9CY_000244"
     CDS             3044..3364
                     /locus_tag="ACG9CY_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS4936790.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(3476..4333)
                     /locus_tag="ACG9CY_000245"
     CDS             complement(3476..4333)
                     /locus_tag="ACG9CY_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4936791.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(4441..5745)
                     /locus_tag="ACG9CY_000246"
     CDS             complement(4441..5745)
                     /locus_tag="ACG9CY_000246"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4936792.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(5742..6251)
                     /locus_tag="ACG9CY_000247"
     CDS             complement(5742..6251)
                     /locus_tag="ACG9CY_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936793.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(6426..8561)
                     /locus_tag="ACG9CY_000248"
     CDS             complement(6426..8561)
                     /locus_tag="ACG9CY_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS4936794.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(8644..10299)
                     /locus_tag="ACG9CY_000249"
     CDS             complement(8644..10299)
                     /locus_tag="ACG9CY_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4936795.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(10301..10615)
                     /locus_tag="ACG9CY_000250"
     CDS             complement(10301..10615)
                     /locus_tag="ACG9CY_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4936796.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            10785..11045
                     /locus_tag="ACG9CY_000251"
     CDS             10785..11045
                     /locus_tag="ACG9CY_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS4936797.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            11059..11373
                     /locus_tag="ACG9CY_000252"
     CDS             11059..11373
                     /locus_tag="ACG9CY_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS4936798.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(12103..12657)
                     /locus_tag="ACG9CY_000253"
     CDS             complement(12103..12657)
                     /locus_tag="ACG9CY_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS4936799.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(12783..14712)
                     /locus_tag="ACG9CY_000254"
                     /pseudo
     CDS             complement(12783..14712)
                     /locus_tag="ACG9CY_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(15018..15734)
                     /locus_tag="ACG9CY_000255"
     CDS             complement(15018..15734)
                     /locus_tag="ACG9CY_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4936800.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(15735..17558)
                     /gene="glpO"
                     /locus_tag="ACG9CY_000256"
     CDS             complement(15735..17558)
                     /gene="glpO"
                     /locus_tag="ACG9CY_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS4936801.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(17572..19068)
                     /gene="glpK"
                     /locus_tag="ACG9CY_000257"
     CDS             complement(17572..19068)
                     /gene="glpK"
                     /locus_tag="ACG9CY_000257"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS4936802.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(19204..20664)
                     /locus_tag="ACG9CY_000258"
     CDS             complement(19204..20664)
                     /locus_tag="ACG9CY_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4936803.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(20654..21949)
                     /locus_tag="ACG9CY_000259"
     CDS             complement(20654..21949)
                     /locus_tag="ACG9CY_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4936804.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(22281..22958)
                     /locus_tag="ACG9CY_000260"
     CDS             complement(22281..22958)
                     /locus_tag="ACG9CY_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS4936805.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22988..24079)
                     /locus_tag="ACG9CY_000261"
     CDS             complement(22988..24079)
                     /locus_tag="ACG9CY_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS4936806.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(24180..24614)
                     /locus_tag="ACG9CY_000262"
     CDS             complement(24180..24614)
                     /locus_tag="ACG9CY_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4936807.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(25198..26655)
                     /locus_tag="ACG9CY_000263"
     CDS             complement(25198..26655)
                     /locus_tag="ACG9CY_000263"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4936808.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(26652..28547)
                     /locus_tag="ACG9CY_000264"
     CDS             complement(26652..28547)
                     /locus_tag="ACG9CY_000264"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4936809.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(28605..30380)
                     /locus_tag="ACG9CY_000265"
     CDS             complement(28605..30380)
                     /locus_tag="ACG9CY_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4936810.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(30430..31926)
                     /locus_tag="ACG9CY_000266"
     CDS             complement(30430..31926)
                     /locus_tag="ACG9CY_000266"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4936811.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(32120..33742)
                     /locus_tag="ACG9CY_000267"
     CDS             complement(32120..33742)
                     /locus_tag="ACG9CY_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4936812.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(34070..35383)
                     /locus_tag="ACG9CY_000268"
     CDS             complement(34070..35383)
                     /locus_tag="ACG9CY_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4936813.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(35628..35852)
                     /locus_tag="ACG9CY_000269"
     CDS             complement(35628..35852)
                     /locus_tag="ACG9CY_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936814.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35872..36294)
                     /locus_tag="ACG9CY_000270"
     CDS             complement(35872..36294)
                     /locus_tag="ACG9CY_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4936815.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(36436..37146)
                     /locus_tag="ACG9CY_000271"
     CDS             complement(36436..37146)
                     /locus_tag="ACG9CY_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS4936816.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(37164..37661)
                     /locus_tag="ACG9CY_000272"
     CDS             complement(37164..37661)
                     /locus_tag="ACG9CY_000272"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4936817.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(37698..38150)
                     /locus_tag="ACG9CY_000273"
     CDS             complement(37698..38150)
                     /locus_tag="ACG9CY_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4936818.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(38285..38890)
                     /locus_tag="ACG9CY_000274"
     CDS             complement(38285..38890)
                     /locus_tag="ACG9CY_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4936819.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(39340..39960)
                     /locus_tag="ACG9CY_000275"
     CDS             complement(39340..39960)
                     /locus_tag="ACG9CY_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4936820.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39962..40831)
                     /locus_tag="ACG9CY_000276"
     CDS             complement(39962..40831)
                     /locus_tag="ACG9CY_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4936821.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(40854..41261)
                     /locus_tag="ACG9CY_000277"
     CDS             complement(40854..41261)
                     /locus_tag="ACG9CY_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4936822.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(41280..42569)
                     /locus_tag="ACG9CY_000278"
     CDS             complement(41280..42569)
                     /locus_tag="ACG9CY_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4936823.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(42596..42898)
                     /locus_tag="ACG9CY_000279"
     CDS             complement(42596..42898)
                     /locus_tag="ACG9CY_000279"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4936824.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(42923..45016)
                     /locus_tag="ACG9CY_000280"
     CDS             complement(42923..45016)
                     /locus_tag="ACG9CY_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4936825.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(45505..46269)
                     /locus_tag="ACG9CY_000281"
     CDS             complement(45505..46269)
                     /locus_tag="ACG9CY_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS4936826.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(46380..47249)
                     /gene="mdcB"
                     /locus_tag="ACG9CY_000282"
     CDS             complement(46380..47249)
                     /gene="mdcB"
                     /locus_tag="ACG9CY_000282"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS4936827.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(47274..48350)
                     /locus_tag="ACG9CY_000283"
     CDS             complement(47274..48350)
                     /locus_tag="ACG9CY_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4936828.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(48374..48967)
                     /locus_tag="ACG9CY_000284"
     CDS             complement(48374..48967)
                     /locus_tag="ACG9CY_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS4936829.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(48971..50647)
                     /gene="mdcD"
                     /locus_tag="ACG9CY_000285"
     CDS             complement(48971..50647)
                     /gene="mdcD"
                     /locus_tag="ACG9CY_000285"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS4936830.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(50637..50936)
                     /locus_tag="ACG9CY_000286"
     CDS             complement(50637..50936)
                     /locus_tag="ACG9CY_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS4936831.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(51038..52684)
                     /gene="mdcA"
                     /locus_tag="ACG9CY_000287"
     CDS             complement(51038..52684)
                     /gene="mdcA"
                     /locus_tag="ACG9CY_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS4936832.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(52691..53602)
                     /locus_tag="ACG9CY_000288"
     CDS             complement(52691..53602)
                     /locus_tag="ACG9CY_000288"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4936833.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            53722..54579
                     /locus_tag="ACG9CY_000289"
     CDS             53722..54579
                     /locus_tag="ACG9CY_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4936834.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(54766..55128)
                     /locus_tag="ACG9CY_000290"
     CDS             complement(54766..55128)
                     /locus_tag="ACG9CY_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS4936835.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT        15513 a        11847 c         9391 g        18420 t
ORIGIN      
        1 aaacaaacct tcactcgcaa gccgagagta aggtttgttt ttcgcattgc ttatttcgtg
       61 ttgtctattg aaatttttcc gcacctagct tttgatatag tggtgctagt tcttgtaagg
      121 tttcagcggt tatttgctca acgtgttcga tttcttgcgg atagaagaat agtttaccca
      181 tttgaaaggt tcggtctctc atcgcttttt tcaactcttc ttgtttcttt gtatcggaca
      241 aatagtcaga caaagctaag tggtcgtcaa gttcatgttc tggttgcggc catatgtaat
      301 aatccttgat atcgactgtt ttgctccact ctttcagtat cgcaagcaac tggttatacg
      361 ctgcaatgct accatatcgt tcttcgcttt tataatgttg gtacatcaaa tagatttggt
      421 attgtttttg attcaacaaa actgctagac aggcattctc gtcttgacga gattcgcttc
      481 ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt cgtccagctt tcgattttag
      541 gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt ctggagctga atctctttcc
      601 atttttgcca gtgttcttta tattctttct tgatttgttc aatcttatct gttggatggc
      661 tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa caactgataa tctgattttc
      721 caagttccat tgtttcgcct ctttccgtta tcttttgtat tctacctgaa ttttgcgata
      781 tggacaaata gaggttgtga aaaagctgtt ctgcatcaag tcataagaac agccaaacaa
      841 aaaactgcga tgagcatcac cgcagtttta ttgaacttaa ggttgattga tcacatctaa
      901 aagcgttgaa aataaaatgg cactagaagc agctcctggg tcgatatgtc cgattgcacg
      961 ctctcctaga taggaagctc gtcctttttt agctaccaag actttggtgt gttcttttgc
     1021 ctggtcaatg atttcttttg ttaaattccc tgccttcaat gcctctgtga ctggcagcca
     1081 aacatctaac atcgttttat cgtcagcttc tgcctttcct cggctttgaa tacccgctgt
     1141 tccctgctcg atcacttcgc ctaatttttc ttgagaatca atagaatcga cctcttttgt
     1201 cgctttcatc atgttcatga atgcagatcc gtataacgga ccagaagccc caccgacttt
     1261 cgatatcatc gccatagaga atactttgaa tgtatcgctg attgtttcag gatgatcggc
     1321 ttgaaaagct tcttcataag ctgaaacccc tcttcccatg ttatttccat ggtctccatc
     1381 gccaatcggg gtatctagat cactgagata ttgtttgttc tgattgattg cctctgcaaa
     1441 ttgcgtgagc cattcttgga tttcttgaac ggttagtttc atattatcac ccttttatct
     1501 tgagattttt taccatgaaa tcgtctgtac attgctttct aaagaatctt tccattgtgt
     1561 ctccgtcaag tcgatcagtg ttagagaaag accttgcata tctagtgagg tcataaaatt
     1621 accgactttt cggaaaacta cattgacgcc tctttcttcc aacagagcta aaacatcatt
     1681 catgaacacg aactgttcca tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc
     1741 ttcttttggt aaattcgagt aatcatctaa aatcttagta acaagttcct ttgccagtac
     1801 tttagaaggc tgcatctttt ctcgacgata gcctggctca ccgtggatac caacaccata
     1861 ctcgatctca tcttctccta gttcgaatcc tggtttccct acttctggta cagttgctgc
     1921 tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc ttttctccca gttctttcaa
     1981 ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg attttgtgta caaggacagt
     2041 acctgcaact ccgcgttttc cagcagtata cgtactattt tcaacggcga tatcatcatc
     2101 tacgatcaca ctttctactt caatatcgtc catagcagct aattcctttg ccatatcgaa
     2161 gttcaatgca tcccctgtat aattcttaac gatcagcaaa actccttttc cactatcggc
     2221 ttccttaata gccgtctgaa tctgatctgg agttggagaa gtaaacacat caccaagcac
     2281 cgctgcactt aacatccctt cccctacaaa cccggcatgg gatggttcat gtccgcttcc
     2341 tccaccagaa accaaaccaa cttgttccgc tttctgattc ttggctacaa cacgggaaga
     2401 ttctacacga tgaacaagtt ctggatagct ttttactaat ccttccagca tttcttcgac
     2461 aatattgctt ggatcattca tgattttttt caatttattc ccatcctttc tttccggcat
     2521 tttgtgcttc tgttaagatt tgctgagtat catctgttat accggcagtg atggctgctg
     2581 caaaagctcc ttccactaaa ggcgcatcca ccagatgata acgttgctgc tgctcttctt
     2641 ccaacatgtc ttttgccagt tccgagttca ataccgcgct accgatatcc gcaaaaacaa
     2701 aataactagc tgcatcgctt gattcattga ctgcttctac gattttcatc gggtctgatc
     2761 cgatctcgcc atttattccg ccgagagaaa aaatttgcac ctcttcggat ttttgcatct
     2821 gctcaatcat ttctttaata ccatctgtta ctttttgact atgagaaata agcaaaatac
     2881 tttttttcac aagaatcttc ctttctttta catagttgct aacgattggt ttcgctttca
     2941 ttaatgaaca attcaagtat ataataaatt tctaattttg ggaaaatatg tgtagtgctt
     3001 cttgaagttt gttatacttt aattaaaaga gaggagtgat cgaatgatta caacaacaac
     3061 gaacagtgta gagggaaaag ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat
     3121 tactgggatc catgcattta aagacttgga ggcggggttg cgaaatatct ttggcggccg
     3181 ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa gaagcacttg ccgaaatggc
     3241 cgaacgtgca aaagcattag gagctgatgc gatcattggc gtaaaaatgg actatgaagt
     3301 attaggcagc gacaatggca tgctgatggt cacatgtagt ggaacagctg tctccacgag
     3361 ataagtaggg gacatctgtt ttaataacta aagagagatg ggacatgtag tggcactcaa
     3421 gaattagatt tttcagtcta acttcgaggt actctacctt cccatcctct cttttttatt
     3481 tctttttgcg gatgattccg taagctgctg cgactaacac acccgcccct gctaaggaat
     3541 aactgacaga agctgttttt tcatttgttt ttggcaaagt ctgacttgtt gatttggatg
     3601 gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga ggtcactgat ttttctcctg
     3661 ccgaatgcaa aatatctttt attcgttgga aactagctgc taagtcagct ttttttgctt
     3721 caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc gtggattgct tgactggccg
     3781 ctgtgatatc cgcttgagtt tgacttagct ttccagaagc tgtttgagca acctgattta
     3841 attgctgatt gaattctttt gcttgttgga tcttcccgtc aagatctgtt tggacagctt
     3901 ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg tgcacggtca tttcccactt
     3961 tatttttaag atcagctaaa tcttgatcga ttgctgcttg gtcttctttc aatttgtttt
     4021 ttgcagcttc taatgcttct tcgagttctt ttcttttatt ttccaagtct tgcaaatgat
     4081 cgatttggtc agaagaggta gaactattta ttgatgtatc ttctgaagat gaaatcgttt
     4141 ctcctgaatt atctgtcggt acattggaag tcatttctgt ttccgaggca ccgttttcag
     4201 caccagaaga gcttatggtt gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa
     4261 aagctgtctg tcccaataag aaaatactgg aaacagcaat tggccagatt aacggcttca
     4321 ttcttttttt catcactttt cccatcctta ctacttttta actaatttct tattataagc
     4381 aaaaatgttt ggaagaacca taaacacaac aggctgtttg tctaaaaaac ggtgcatctt
     4441 ttattttttt gtacgttccg ggcttttcca tgtcccgctt ccataaccta aaatcgtttt
     4501 gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc actatccaga acaaaagcat
     4561 atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt cgatcatcta caatcagcga
     4621 agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt
     4681 aaaagtaata taaatctgtt cgtagtttcc ttgatataca taatgcccga ttaatccagc
     4741 aaaaagaact acggaaatac aaaagaatat tgaccagaca atactcaatg tttgatcaat
     4801 gaagatgatc gttcttccaa tatgtttaaa aggatggagc atcacttttt tgaaattcgt
     4861 cagccagact tctgttccac cttttgccca acgttttctg tgtcgatata gcatcttcaa
     4921 cgtcacaggt acttccatga aaaagataat ctcaggagca aaaacagata accagtcatt
     4981 taactgatga tcccaagcaa tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc
     5041 cacatcgatc aaggcttctt ttcgatacat cgtatttgct ccactgtacg cataaagacc
     5101 accgaaaaca gctgactgtg ttcgtttaat gatgcccacg atacttgaga attcaaccgt
     5161 ttgagatttt gcaatcaatt tggtacgatt ttggacatcc atatttgcag tgaccgcagc
     5221 aatatgtctt gcaccagggc gaatgaaata attcacatac ctaatcagtg catctggctc
     5281 cggtaccgtg tctgcatcat tgcttaaaat caatttccct tttgcaaagg caagtccgat
     5341 attaaacgcg tgggcttttc ctttattttt ttcgatccga accacacgaa gattagcgta
     5401 ttttttcatc agtctagcta atatttctgg tgtttggtcc gttgaaccat cgtctgtcac
     5461 taatacctca tagttgtgat aattcaattt cgtcatcaaa tactcgattg tatattcgat
     5521 cacaatttct tcattatggg ctggcaccat gatcgtgatg aacggttcga cactcaaggg
     5581 aacatctacc cattcttttt gcttatgttt gtataaaaac acataacacc agacaccaat
     5641 aaaccataca aatccgccta aaatcggata agaaacgaga aaaaaataca taatagacaa
     5701 acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag ttccacccca
     5761 ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc ggagacagaa
     5821 taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata cacttcttca
     5881 agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt atttactttt
     5941 ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag gaaactgaaa
     6001 aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga taaaaaagcg
     6061 tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac ccatataaaa
     6121 ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat ctttaaccag
     6181 taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc ttctcttgtt
     6241 acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt accatacatt
     6301 tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc
     6361 tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta ttttttatct
     6421 cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga tatcgccgtg
     6481 tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata taagattaca
     6541 tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt ttctaatcca
     6601 tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt ttgggtgata
     6661 actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt caacaattct
     6721 tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat cgatacgatg
     6781 tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc tccgctagaa
     6841 cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc
     6901 ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc acctactaaa
     6961 gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag ggtataatct
     7021 tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc aacaagtaaa
     7081 tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg ccaagcataa
     7141 gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc cacccatgcc
     7201 atttcttcga tttgtctggt tatttttcga tatttttttt gtaaaagttt ccttgcataa
     7261 gctacaatac tttgttccga atcatcagaa atcaatttac cgaataattc caactgccta
     7321 tcttctgtca acgagagtac ctgattctgc acagcaggac tttttgcttg tttcaaaaga
     7381 tgattcttcc attcgctcat cagtgccttt ttcgttgctt gaagcctatc gatcaattta
     7441 ggatgagcag gaactttctc atatattttt tcgatcgttt ttttagaaag gactgttttt
     7501 ccatttttag taagtccacg gataaaattc ggatgatcta aaaacaaagg atctgattgt
     7561 tttagattat cgataaactt tttgctacgc aaacgttctg tctgctccga tacattttct
     7621 cccgtgatac gtttgaaata agcctcttca ccttctattt cctctgctga aagctgtgaa
     7681 accagctgta tgatggttag attcagtgga tttctttcac ctaatgatgg taggacattc
     7741 gaaatatagt cgataaaccg atgattaggt gatagaatca gcaagtcatc ggatgtcatc
     7801 gtatctcgat ataaatagag cagatacgca atacgctgca tgatagcaga ggttttacca
     7861 cttcctgcta cgccatttac caagatattt tgacttttca catcccgaat gattgtgttt
     7921 tgttccgatt ggatcgtagc agtgatagcc ttcatttcat tggtctcatc ttggcctaag
     7981 acttctaata atacgtcatc ttgaatagct acagacgtat caaaaaactt gaccaaacga
     8041 tctctttcca aaagaaattg tcgtctttct ttaataaccg tatcgatttt attttgacga
     8101 accgtataag aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc
     8161 caatcataaa caagattttc atctgcatca tctgtgaaat ttgctgtacc gatataaaag
     8221 gattcttttg gctcattatc cagaaagtct acaacgactt ttccaaagta gggacttttc
     8281 aataaacgtt cgatctttgc tagctgattt tctgcgtttt tctgtttgat attcatctga
     8341 tcgatttctc tatttttcat ttcaatcatc gaaaaagtat ccaggttatc taagtaactg
     8401 tcaaaattca atcgaagctc gccagccatt tctttcaacg actggtttcc ttctattttt
     8461 gccgtatcca tcgattcccg cagttcgttt tttttatcaa ttagcttttg atacacgttc
     8521 ttcagatatg cttcttctaa ctgtttttgt gtgttgacca tacacattct cctctttttt
     8581 taaagtggtg gaaccgatta gtttacctct tttaagagtc gaatgcaagc tttttaacga
     8641 gtatcatttg ttaggatata tcagttcttc aggtcggaca gtttgatata aagaaaatgc
     8701 accatcaagg ttttttacgt ggtagccaga ctgcttcaag attctttccg ctagatagct
     8761 acgtaatccg ctatgacagc tgacgatata ttcttgttgg ctgtctaatt catttaatcg
     8821 gtcccgaagt tcattcaaag ggatatgttt ggcttccttg aatttgccct tttccaattc
     8881 ttctgctgtt cgcacatcta ataaaatttt tccttttgct agttctgtcg gcaattcatg
     8941 ccattgtacg gtttcactaa gcccttctgc aatgttcatt gctgcatatc ctagcatgtt
     9001 tactggatct ttggctgagc caaatggcgg tgcatacgtc aattctaatt ccggtaaatc
     9061 gaagatcgtc aaatgccctt tgattgctgt tgctaaaata tcgatccgtt tatccacacc
     9121 ttttgcccca acaccttgtg caccataaat ctcgccagtc tctggatgga agatcaactt
     9181 cagtaaaata tctgttgctt ctggataata gcttgcatga tcttttcctg atacatgaat
     9241 gacttcaaaa gacagttcgt tttctttggc tgttctttca cttaaaccgg tggatgcggc
     9301 agtcattcca aacgttcgta taattgctgt tccaataccg ccttgatttc ttcgagaaat
     9361 tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag caagagaaat
     9421 aagtgcatct tttccagtga tttgctgttt tacaacgata gcatccccca ccgcataaat
     9481 atcaggaata ttcgtttgat aacgttcgtc tactaggata cccccgcgta tgcctaattc
     9541 gattccagcg gtcactgcta gctggttatc aggacgaaca ccaatggata aaataactaa
     9601 atcagcaggg atggtacgcc catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc
     9661 agcgacagca tgagaagtaa tcacttctac tccttgatga gacaattctt tttctataaa
     9721 agctgccatt tcttcatcca gtgttggaag aatatgtggt gcttgttcga tcaccatgac
     9781 ttctaaacct cttcttttta ggttttctgc catttccaat ccgatgaatc ctgcaccaac
     9841 gatcacggca cgctttggct gtttttctaa tgcatgcatc actttatcga tatctggtac
     9901 atttctaata gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg gaacaactgg
     9961 ttttgcacca ggagataaaa ccaatgcatc gtactgttca atgtgttttt gaccctcatg
    10021 tacgacttct accgttttgt tttcgggaaa gatggctgtc acttcatgat ttggacgcac
    10081 atctaaacaa aaacgagcgg ataatgattc tggcgtttgg acaagaagat tttcccgttc
    10141 actgatttct cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa catacggtcc
    10201 tttttcaaaa acaacgattt ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga
    10261 cattccacct gctacacctc cgacaataac gattttcatc ttatttccct cctactgttc
    10321 gatcaaacca ttgattcatg ccaccacgta cattcactac atcataacca gatttcttta
    10381 attctttagc tgcttgttta ctcctcatcc ctgattgaca aatcacatag acggtctttt
    10441 ctttgtctcc atcatatctg tttatacttt gtaatggaac atttttagct ccttttatat
    10501 gcccgccccg gtattctgca ggtgttctaa catcaagtaa cgctggattt tccaaaagct
    10561 tttcctttaa ttctgggact gttatcgatt ttattgattg aaatagcaaa ttcattttct
    10621 gcacctcatt tacattttta taaatacctc atggggtata ttaaacagga gctttattaa
    10681 tatgtcaacc agaatgactc ttcatttttt tcactacttt ttatatttgc ataaaatacc
    10741 gataaggggt attatctaat tataaaaata gaggagaatg tgttatggag attgataata
    10801 aaaatatcct gaaccgtttg aaacgtacag agggacaaat tcgtggtatc caaaaaatga
    10861 tcgaagacga aaaagaatgt atggatgtca tcactcaatt aagcgctgtt cgttctagta
    10921 ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa aaattgcttt gagaaacctg
    10981 atagtaatcc agaagaacaa gcggctaaac tagaacaagc gatcaagatg attattaaaa
    11041 agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg atgaattaga aaaatggtca
    11101 gaaacaggga aaaacgtaaa aaacctgata aaagcttctc cagaaacgac gattgtcgta
    11161 tctgtaaatg gcatcgcgat taccggttat ctagactctg ccaatgcaga attcttagat
    11221 ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa
    11281 agttctttac ctgaacaagt aatcgttgta ccagcaggtg tccttgattt agttgaactg
    11341 caatcccaag gttatgctta tatcaagcct tagtcagtag aggttgcgac agaagtgatc
    11401 agcttcaaga aatttcggct tatttttcga agaagctact tctggaaaac cgtgaatcaa
    11461 aggttgtgaa aacgctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagcttct
    11521 catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag
    11581 gaagaggttg tgaaatcagc ggtttgacct aagtattaga gaaaaaaacg ggaaaatagc
    11641 tcctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga acaccgttta
    11701 aggaagagat tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg
    11761 gatttctaca tttttgcata aatttggctt aataccgaga aactggcttt tgaataccgt
    11821 ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagaaac aaacggaaaa
    11881 atagctgctc atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc
    11941 gtttaccaag aggttgtgag cctaccattt aactccgaat cacaagaaat aatttcaaaa
    12001 acaacttctc ctgtttttgg ttcggttggc ttattatcga gaagtagcta ctgggagtct
    12061 atttatcaaa aaaaggaatg cgacataggt cacacccctt ttttattttc tttgaaacat
    12121 ctttcggtac tcgacagcat acaagacaaa agattgtaaa agaccgtaag tgatgacggc
    12181 aaaaatgact ctcacccaca gcgttggtaa ttctgggaaa agcagcaaaa ggatcatgcc
    12241 aacatagatg acagcagtcg ctactttgcc ataccacttc gctccactta atttgatatt
    12301 tttagatagc ccgattaatc cagctacaaa cataaagcct tcttttatca agaatagacc
    12361 aaacaacagc caaagccacg gtcgatacca tgccatagac aaaatcagaa aaagttgtgt
    12421 caatttatct gcaaacggat caagcacctt ccctaagtct gttacctgat tatatctccg
    12481 agcaatgacc ccgtctagat aatcagacaa acctgagaca acaatgaccg ctcctgctat
    12541 ataaaatgac tgtctcagca caaaataaag ataaatcggc agcaaaaaaa tacggaatat
    12601 cgataaaaga ttcggaatcg tttgtatttc tttacgccag tctgtgctta atttcatttt
    12661 atcgccctac ctcctcagca ctcttttctt tttattttag cttacacggg gaataaaacc
    12721 aatgatttca ctgtttttat ttttctttac agacactgat attatgagct aatttgagat
    12781 aatcacttgc tgttttcatc gtttactctt ctttcttcct gtcgtttgcg catttctttt
    12841 actagtactt tatgagcttt ttttgatcct cgctctaggt cacgctgccg tttacgtgtt
    12901 ttataatcga taaacagact gtctaaatcg taacctgtcg ggtacagatc ttctgctgaa
    12961 cgcaccagac gaacacgctg acgaggcacg tggattattt ttttgtctaa atatacatca
    13021 atcgtatctt cacctatatc ttcataaact aacgcttctt tttggtattc attgacaaat
    13081 atccgatcac cttttgcaaa gagctgacgt tcttttttat tctccttcct atttgtaact
    13141 gctgtcttag gaagaaattc ttttttctgt gtccgatagg ctttttccgc caagtaacgc
    13201 tctgcttgat ggatcaattt catcgacata tccattttat gagcaatcca caaagcttgg
    13261 ctttctcctg tttcttccaa cccgcaactg atatttagga cgaagtgctt cccgatcgaa
    13321 agccatagca gccggcacaa aatcttcatg atctccggcg aatttcttga tttctccata
    13381 atgggtcgtg gcaatgatca atgccccttg ctcatacata gattccatga ttgcgatcgc
    13441 tagtgcagcc ccttcatttg gctctgttcc actgccaatc tcatctaaaa gtaccaacgt
    13501 attccttttt atctttttca aaatagcagc gatgttttgc atatgaccag aaaatgtact
    13561 caacgcattt tctaaatttt ggtggtcacc gacatcgacg aaaatctcat caaagacagc
    13621 aagctctgtc ccttcttttg ccggaacttg cataccgaac atcgccatca aagtcaataa
    13681 accgactgtc ttcaacacga ctgttttgcc tccggcattt gctccggtga taatcaagcc
    13741 tcgatagtct ttaccgattt caacatccaa tggtaccgca tgctcaggta aaaatggatg
    13801 tctgccttgc ttgataatga tatgttccga tttattgatt ctaggtgtgc ttccattgat
    13861 ttctcggcta aattttgccc gagcaaagat gatatctaac accgtgatcg tttcgatcaa
    13921 taaatcgatc acttcttcgt tttctgcgat agcaccagtc aatgccgcca ggacttgata
    13981 ttcttcactg atttcttcag cttttaacaa ctgatagtgt tcattcaatt tagaaatcac
    14041 agttggttca ataaaaaccg tcgttctttt atttgattca tcgataatcg ttccatctat
    14101 tttattttta tagctggctt tgataggaat cgtatagtat tcgccttttt gaacaatcat
    14161 tgcttcttgg atcatttctt tgtttttcgg atgacgcaag aatttcagta attttgactg
    14221 gatttctttt tcgattagtt gtaattgttt gcgtacttta cgcaaattac gtgacgcgtc
    14281 gtcactgact ttattatttt ttattttctg gtcaatcaac tcttctacat ttatcaaatc
    14341 aggaagatgt ttgctataag caaacaacaa gggtgtctgg tattgattct tatcaaaaaa
    14401 ctttgtgatc atccgactgc ttctaagaaa atccgcatat tcgatcaaat cggttggctg
    14461 cagaatcaac ccttttttga cttgttctgt cagtgcatcg atcctcggca accccataaa
    14521 tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct cttgtttctt cttgacgatc
    14581 caagacagtt tgtaggtttg tcgagaccgt catatcgctg attcgttttt tgctatagtt
    14641 tccaattgct cttgcttgga tttccttttt gatccgatca aattgtaatt gactaatcat
    14701 ttcgttgtcc atattctctc cttttcttga agagatacct gtcccaaaat aaataagggc
    14761 ctagagataa ataaaaggat agaacacagc ttaaaaaagt catgtcctat ccttattaga
    14821 atacaaagat attgttttcc tgacaggtac cgtttcataa gcacaccaaa taaactttgt
    14881 ttattggcta ttcgacttac tgattagata ccagctacac tcacaaaaca taccccattc
    14941 ttctaggtct tatttatttt ccacttaact atagcaagac acattttggt tgtcaaactt
    15001 tagtatgttt tatagattta caccattaat gaatagacac ctaccgcgat aattgctccg
    15061 atcattggac ctgcgattgg tacccatgaa tatgcccaat ctgaagttcc tttattagct
    15121 attggcaata ccgcatgtgc taatcgagga ccaaagtccc gagcagggtt gattgcatat
    15181 cctgttggcc ctcctaatga aagaccgatg gctaagatca atccacctac agcgaatacg
    15241 ttcgttccag gtgcaaattc gttttgtcca aaagctagca acccaagaac taatacaaat
    15301 gtaccaatta attctgtgat cacatttgct ggataattcc gtatagcagg acctgttgca
    15361 aacgttccta aaatcgcgct ttcatctttc gttgcattcc aatgtggcaa ataagaaagc
    15421 caaacaagaa tggcccctgc aaaagctcct aaaacttgcg caatgatgaa aggaacgaca
    15481 agattccaac taattgcccc tgtcatcgcc atagctagag agactgcagg attcaaatga
    15541 gctgggccca tgaatccaga aatatagaca gccattgtta cagcgagtcc ccaaccaaat
    15601 gcgatgacaa tccagccaga agcctgtgct ttacttttgt ttaaattcac agccgcacac
    15661 acaccatctc caagcaaaac taggatcagt gtcccaataa actcccccat gatttgagtc
    15721 atatcactgt tcatttattt ctcccccttt agttcccgca gatctgattc tgcaatcaat
    15781 gcttctaaac gtttagtctg ttgcgccttc tcctcttctg tccattcaaa atatccagca
    15841 attgcatcga tcacaggttg cttgatctca tcaagctgat ctcgttcaaa caacagatga
    15901 tttgtacgac gaatgagata atcgcctggg gccaaaacca tttcttcttc taaaccataa
    15961 cgtaaccgag ctgactcagc gagactcaaa ccaggataag gtgccatttc ttttgctaat
    16021 tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga tataagtagc atcttcttcc
    16081 tctaaaccgg ctgctactcc ttcttttgct aattctgtca ctgtttcttc taatttagtt
    16141 ggatcgaagt ttccacctga aatctgatat tttttagagt cgatttcttt cgtctctatt
    16201 ccgtattctt cttttaataa ttggcgtatc aatcgcaaag ctccttctgc catcttacgg
    16261 taatcggtga ttttcccacc agataaagtc agcaaaccat ctggttctct ttctaaagaa
    16321 ctacctctgg aaatcgtaga aggtgctttt tcatcacggc tgttttccaa atggttcaac
    16381 acgtcttcta cttcagcacg agaaactttg ttttccttat attcacttac tgtatcgaca
    16441 actttattga agctcttgtc tgaaatcgat ccattatctc caccattgta atcagaacca
    16501 gaattaccaa tcaacagtgg acgaagtcct gcccagcttg cttcgatatc attcaatgtg
    16561 atatttgctt ctggatagcg atgattgatc acatccaata gataatctac atcttcttgt
    16621 gtgactttcg gatccgtaaa atctccttgg taatccgtat ctgtcgtacc aaagtaagtc
    16681 ttgttttctc ttggaatagc aaaaaccatc cgcccatctt gctttcctgt atcgaagtat
    16741 gtgggttgcg gtacaggcag ttttttcgca tctacaacta aatggatccc tttagttgga
    16801 cgcattttag gagagactgg acgcgtaaaa ttcaagtttc gtactttatc tacccaagga
    16861 ccactcgtat tgatcactaa ttttgctttg atttcgatca cttcatctgt cagcaggtca
    16921 cgagctttga cgccaacgat ttgatcgcca tcatataaaa agccaaccgc tttcatttta
    16981 cttactagat aagccccatc ttcggcagcc tttttgatat tatcaatcac taaacgggca
    17041 tcattgttgc ggaaatctag atagacaccc gcgcctttta gcccttcttt tttcaaaaac
    17101 ggttctcttt ccaatacttc ttctggtgtc agcgtataat tctcatattt ggttcctgtc
    17161 acatttgcca gtttgtcata aaggtccatt gctactttga ccgagaacat attgaaggtt
    17221 gttgctcctt cgccctcata gattggcaaa agcattggat ctggttttgg gatgtgtggg
    17281 gcaatacttt gtacgactgc acgttcacca actgtgtcag ctactacttc cacatcaaat
    17341 gtcttcagat aacgaatacc gccatgaaca agtttggtcg agcgagagga agtcccttct
    17401 gcaaaatcct gcatttcgat caatcctgtt ttgattcctg atgctgctga ctggactgcc
    17461 acaccggcac cagtgattcc gccaccgatg atcaacagat ccaattcttc ttgctgcatt
    17521 ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat ggatttcctc cttattcaaa
    17581 tgcttgggta gcttttactg cttttttcca accgttgtaa agtttttctc ggcgctcttc
    17641 ctccatttga acttcaaata gcttgccagc ttcataaaat tcgcggatct cgtctgtatc
    17701 tttccaataa ccgacagcta atcctgctaa aaatgctgcc cctagcgccg ttgtttccaa
    17761 gttttctgct cgttggatcg gtacattcaa gatatctgtt tggaattgca tcagatattc
    17821 attgtttgct gcaccgccat ctactttcaa tacaggaatc ttgattccag tgtcatcttg
    17881 catcgtatcg atgatatctc tcacttgata agcaatcgac tgtaaagtag ctttaatgat
    17941 atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc cctcgtgctg cctgatccca
    18001 ataaggagca cctagcccaa cgaatgcagg aacaacataa acctcatctt cgcttgtaga
    18061 gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac atttgaagtc catctctcag
    18121 ccattgaatc gaagaaccag ccacaaaaat acttccttct aaagcatagt aaacctttcc
    18181 atttatgccg taaccaattg tcgtcaataa attatttttg gataattgcg gtttttcacc
    18241 ggtattcatg acaataaatg aacctgtacc ataagtattc ttgaccatgc ctggttcgaa
    18301 tgccatttgt ccgaataatg cggcttgctg gtctcctgcc atccctgaaa ttggtgcttc
    18361 gcttccataa aaatgataac cttgcgtata accataaact tctgagttag aagtgacttt
    18421 tggtaacatc actcgaggaa tatttagtaa atctaaaatc tcctgatccc aatctagctg
    18481 atggatattg aacaacatcg tccgactggc attcgagtaa tcagtaacat gtgcttgtcc
    18541 atcggtcagt ttccatacta accaagaatc gatcgttcca aacatcaatt cgcctttttc
    18601 agctcgttct tgcgcgcctt ctacatgatc aagaatccag cgtatcttcg ttgctgagaa
    18661 ataggcatcg atcactaacc ctgttttttc gtggaaaaat tcttgatagc cttcattttt
    18721 taagttatct gcaattccag ctgactgtct ggattgccaa acaactgcat gatagattgg
    18781 acgtcctgta tctttttccc atattactgt tgtttcacgc tggttcgtta tgccaatccc
    18841 agcaatctga tttggtttga tccctgattc gatgaacgct ccggcaataa ctgactgtac
    18901 agaattccag atttcatttg catcatgttc cacccaactt tcttgcggga aatattgcgt
    18961 gaactctttt tgagaactgc cgatgtgtct tcctttcttg tcaaagataa ttgctcgtga
    19021 gctagttgta ccttggtcaa tcgccatgat atacgttgat tctgtcattg tgattccctc
    19081 cattatatgc aaacgctttc tttaactact cttatcatac tactgtttta aaaaaagaaa
    19141 ctgtcaaaat tttattcttt ttttcttttt ttgacagttt tttattatga agctattttt
    19201 taatcaattc ttttcttttt tcagcgtctc tattcttctt ttcaatcgaa taatgtcgta
    19261 aggtgacgca aattctgaca gataatattc acactgtttt ttgtttctct ggcaaaatgt
    19321 gatcaccaaa tcatattctt taccttcttc ataacttcca atctcaatac cgccgattcc
    19381 cctcagttct ctgatcagat attgttgaaa agcaatacga taaatcggca gactttctaa
    19441 atcgattcca atcactactg gttttgcaat ataaaaatcg atcatcaaaa gaacattggc
    19501 atactggatc atcaggtaac taagctcact atcttcagct cttttcctcg gcaattgctg
    19561 caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag gaatgcccga gcattttctg
    19621 ctgctgttcc aaaagccgat cacgctcata gacttcgatc tttccaacaa agaaaaacca
    19681 ttcattgttg atctgggaaa tttggtagcc tactgccttt tcttctttga ttttcaaacg
    19741 acgaggacga taatgccata acatcgtttc ccgaatgtaa gtatctaaaa cagcggttgg
    19801 taactttttg ctccgtgtca aatcatactg ataaaatatt tcttcgtcta ctatcgaaaa
    19861 agaaacaaaa aagctgtaaa acatcattgt ttcgtataca ttcagttctg ctgctgttcg
    19921 actaagatgc atcacgatga ttggatcgat tgcttgataa agccggtccg actgatagag
    19981 taatttcttt tctttcaaag ttgcgtacgt acttttttca ttcaatagtc tttttttcgt
    20041 aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc gtaaacgttg tttctaatac
    20101 acggtccaat cctagaatga acggtctttt ttctggtgta ttttgcaaaa aaagcggtcg
    20161 ctgatcttcc gggatatatt ggaatagttc ataataaaaa tatcgtatct gtagctcttc
    20221 tccgtataat tgaccgtttt tgatttgtaa tccaaattcc tctaagagct ggttcaattc
    20281 tttgatcttt cgaaaaacag aagactcact gatattaaac gctgtcgcta atcgaacgat
    20341 ggatacttgc tgatgttcta ataaatagac gagcatctta aacttcaagc tttcctgcaa
    20401 taaaaacaat agaatttttt ccaaacttac atcttcgtcc aacacaagca gcgcctgata
    20461 ctcatttcgc tgtacttcta cttttttttc catcattcgg ccaatcaatt caatatcttc
    20521 caaatattga tcaaacgagg cttttgacag ttggacatgc tctctcaaac cattgataga
    20581 ggctgttccc ccttgcagaa caaggtttcg caaaatcagg atctgtaact gctcttttct
    20641 ttctaataat gcttcaatct tcataggcag tattcgccac ctttgaccac accatatctg
    20701 gcacacggta ttctggctgg aaaaatagct catgttcttc taactgttca aacgtccatc
    20761 cttcttctac tgcaattgtc agttggtcaa gaaggtggaa catctctttt ttgctttgga
    20821 gctgtgctcc aagaatcttt ttttcagaat caaatagaaa tttgagttta actttgcttt
    20881 gatttattgc tgtaagcttg tagtctacgt atcctgtttc aattttttgg ggataaaaaa
    20941 tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata gccaaaccac tgattgccga
    21001 ctggacgata ggttccctga tcctttgtct gtgagccact aattgtcttg ctgacatttc
    21061 gtgctgtacg gacagcgtta tttactagag aaacatacat tttttcattt gttggacgaa
    21121 aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt ttgagacgtt tgcaagtagt
    21181 catctaccca gatcgttcca tcatcattca atgttaattg ttcttcccac atctgattgt
    21241 cgggacgaga atgattagcc agcaaaacaa gatcatagtt ctcctttctt ttttccgtct
    21301 ctacaatagc ggtttcattt tcggtcaaag acacaacttg ttcttctaaa aaaagtgaaa
    21361 gcccttgatt tttcatttcc tttactaacg gttctgtcat ttcaggatct agataacgaa
    21421 aaagaattga ctttcggctt tcgtaaagat ggatttgttt ttttcttgat gccaaccctt
    21481 cagctatttc gattcctgct tgtcctgcac caataattgc aattttcttt gcattcatga
    21541 tcttttgctg aagagccgtt acgttttgta tctttttaac tgggtgaata agcgttgatt
    21601 ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc tagaatcaaa cgatcaaaat
    21661 ccaatttttt tcctcctgct aaaacgactt ttttttcttt caatcctatt actgtctttt
    21721 ctgtgtaaac atggattcga tagcggtcag tcagttcttc tttcgtgatc caagaaagtt
    21781 gtttttcact ggtaaccttt ccttttaata tcaaattgaa cccgctggga atccatccta
    21841 acttcgattg cttttcaatc aaatatattt cacttgttgg catcaattgg cgaagataaa
    21901 tagccgcatg aatccctgca aacgacccgc cgacaattac aatgttcatc tgcattcctc
    21961 tttcttataa ggtgttctac tgctaacttc tcaatcagaa aacacagtat tcataactcg
    22021 ctttcaacct tatgatacat agcgatgcag cagaatcaac cctctccatc ataccgtgtt
    22081 ctaggtctgc ttctcaataa tgtactagtg gacctaattg aagagtatca tttctgaaga
    22141 gatgtttcaa aaaatgtact cttataaaag aaaaaaactc gccttttacg tacctctgtc
    22201 caaataggaa acagaaaaga acgaaaagac gagttttagc gtgtgtaatg taatagttag
    22261 aatagacaca tcatttattt ttaattcttt aatgcttcaa caactgcttc gcctaattga
    22321 tgagcagatt gaggattttg ccctgtaaga agccgcccat caatttgaac cacttctgaa
    22381 aaagctgctc cagactgata gtttgctcct ttttctttca aagcatcttc tgctaagaat
    22441 ggtacttcat tcgttgtacc atttgcttcc tcttcttcat tactgaaccc agtcactgta
    22501 cgtccagcta aaatagattt tccagactca tctgtaactg gcaacaatcc tacaacaccg
    22561 tgacaaacag ctgagaccag cccttcattt cgatagattt gtgaagcgac tttcccaaga
    22621 tctattgctt ctggaaaatc ccacattgtt ccgtggccac cagcataata aaccgctgca
    22681 taatcatttg catccacatc atcaggacgt aaggtatttc ccaaggcccg atttctgaaa
    22741 tcttcatctt cataataggc ccaatcgatt tcatccatat tcaaaacact ttgcggttct
    22801 aaaggtacat atccgccttt aggactgaca aaatctgcat cgatcccatt ttttactaat
    22861 acatcataaa aatgagtcag ttcacttaac cacaaacctg taggacgctc gatcttttcg
    22921 tacttttcta catttgttaa tacgatcaat actttcattt tcccatcctc tttctataaa
    22981 aattgcgtta ttgataacgt gctttcaaga aattatataa tgggcttggc aaataaagct
    23041 ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc ctcacggttt tccaataatt
    23101 tctctgccca gtcaggatcg acgatcattt gctgtccaat tgctgcaagt tctgcatatt
    23161 tcaatacctg ctctacatct tcgcgattgc ggatcccacc gacaccaact aaagggatct
    23221 ttccttgaat cgtgttatgg accacttcaa gaatcgattt atcgttgtat ttatcagaac
    23281 gagctattcg atcatacata tttaatgaaa catgcagata atctagtttt gactcacgta
    23341 atcgttcaag aagccagatc gtgtcttcaa aacggatacc tggtgtttca tattcttctg
    23401 gtgagaaacg ataccctacg atgaatggtt ttttagcata ttgttcaacc gctgagaaaa
    23461 ccgttgttaa taattcttca ataaaacgat aacgattttc caagctaccg ccccattgat
    23521 cctctcgtct attcgaatgc ggagagaaaa attgttggat caaatacgta tttgctccat
    23581 gcaactcgat tccatcaaat cctgattcaa tcgctctacg tgtggctttg ccaaatgctt
    23641 ggattgtttc aacgatttct tcttcactca ttgctcgtgg tgtttccgca tcaggacgtt
    23701 ttgctgcaac tgcacttgca gatacaggct gttctccaga taaagttgct cgacttgtca
    23761 ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc ttctccttgg attgctttcg
    23821 ctagctcact taatcgagga atcatttcat catgagcgat gctgagttca cctggccagc
    23881 cttttccgat atcttggaca tttgcagcac cagtaataac agcaccaaga cctttagaac
    23941 gttgtttata ataagtgatc tcatcttcgg tgatcactcc attgaaaaag ctgagttgtg
    24001 tcgtcatagg cgacagcatc aatcggttct tcaattgaac tcctgaaggt aaagtaattg
    24061 attgatcaaa ttgtgacatt gtcattcctc ccataattcg ctctgattaa cctatcgaga
    24121 atcatctatc attaaaccac tcgattagtt gtacatgcaa ctaatttgct taaaaaattt
    24181 tatttttttt gtaacttatc tgttgcatca ttcattgtct gggctaattc tttacttgct
    24241 tcatttccca aaactgcttt gtatgactga tgaagttctt cccagctacc atcgacttcc
    24301 ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg acaagcgacc ttcgctaact
    24361 gttttgacgt atcctttata ttctaatttt tcaacaaaac gagcaatcgt tgatggggcg
    24421 gaatagagca tttgtgcaat atctttttgt ggtattccat ctttttcttt taatatcaat
    24481 aaaataaaac cataagttgg tgccagacct gtccttctaa acgcttcatc agctaattta
    24541 ttcaacatgc gatctagctt tttcacgcta aagaaaagac attcatctaa cgctttttca
    24601 attggctttt ccatttcatc ccctccattg gcgtacatat catagtatga tttttatttt
    24661 ttgtcaaaaa acacggaata atgtccgcct cctctgtcta gatttgaaaa aaagaagtag
    24721 ccatcttcaa agaatttcgg cttgttttcg acaaaactcc ttctggaaca ccgtgaatca
    24781 gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa aattttgtga aaatggcttt
    24841 ccacattttt gcataaattt ggcttaatac cgaaaaactg gcttttgaac accgtttata
    24901 tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag aacagccaaa caaaaatagt
    24961 tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc agctgattgt acaccgttta
    25021 ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa ggagcaattt caaaaaacag
    25081 cttctcatgt tttatgacta tggtgacttg tgatcgagga gttggcagga gaatactgtt
    25141 tattcgtaaa gaaggggctg tgacaagact tttgtcacag tcccttattt ccttttatta
    25201 taggatttcg ccatttgatt ggatgacttg tctgtaccaa tcaaatgatt ttttctttaa
    25261 ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca tagatatatc cataacgttt
    25321 tgacatttgt gaagtagaag cgctgacgat atcaatgcag ccccacgacg tataaccaaa
    25381 taaatctact ccatctttga tagcttcctt catctgctca atatgctgct tcaaatagtc
    25441 aatgcggtaa tcgtcttgaa cggttccatc ttcttctact ttgtctatag ctccgagtcc
    25501 gttttcagct atgaacagtg gtttttgata cctatcatat aattgattca aagcaattct
    25561 caaaccctta ggatcaatct gccagcccca ttcacttgat ggaagatacg gatttttaat
    25621 tcctccagtt attaagtttc cttggacccc ttcccaattt tctggatgag cactgactgc
    25681 actagacatg tagtagctaa aagaaacaaa atcaacaggg taggctgcta agatttcttc
    25741 atcacctact tctattttta agttgacacc tttttcttca aacattcgtt gcgtatacgc
    25801 tggataatat cctcttgctt gaacatcact aaagaacaaa gaagttcttt gctgctcaat
    25861 tgttttttga atatcatccg gatcacaaga atacggaaca agaagctgat agctgatcat
    25921 acaaccgact tgtgaacctt tgataatttc atgacaagct tttgtagcta aagcacttgc
    25981 tataaactga tggtgtgcac cttggtattt cgcttgttcc aaatggggat ggttttcttc
    26041 aattactcct acgctaacaa aagggtgatg ttttacacag ttgatctcat tgaaagtcat
    26101 ccaatatttt actttatttt tatagcgttt gaatactgtt tcagcgaaac gaacgtaaaa
    26161 ctgaataagc tttcgattag gccatccacc atattctgta accaaatata acggcatttc
    26221 ataatgggaa agagaaacga ctggctcaat cccatatttt agacattcgt caaacacttg
    26281 atcataaaaa aataatcccg cttcattggg tgtttcttca tcaccatgag gaaaaattct
    26341 tgtccatgcg atagacatcc gatagcattt aaaacccatt tcagcaaata aacggatgtc
    26401 ttctttaaag tgatgataga aatcatttcc gtgtctttta ggataataat acttgctgtt
    26461 gggatcctgc gccgctttta cttgctgatg cgtcatcata tgccattgat ctgtccattt
    26521 ttctttaggt aaatcgctgt cgaatgtata cacatcagac acagataatc ctttttcatc
    26581 tacatcccat gcaccttcac attgattggc cgctgtcgct ccgccccata aaaatccttc
    26641 tggaaatttt ttcatatcga tccccctaaa cttgctagtt ttcttgtttg attatttttc
    26701 ttatgactag taatgatatt tttgaatcta ctactatttg taacaataac cattgtggtc
    26761 ggatcatatc cctctttgat taattcttgc aaatctactt ccatcaataa atcgccttta
    26821 cgtacttttt ttccatcaga tactgcactt tgaaagtatt tgccatttaa attcactgta
    26881 tcaataccga tatggatcag tatctctgct ccttcttcac tgactacccc aattgcatgt
    26941 ttcgttggaa aaactgttga aacttccccg tttacagaac tgtagatttt tccgtccgtt
    27001 ggaatgatcg caaacccttc tcctaaagat ccactagcaa atgttggatc gtttacttca
    27061 gataaatcaa ttatttcacc tgctgcagga gtacttaaca ctatatcctt tgaggattct
    27121 tcttcgattt catccttgta gccgaaaaga aaagttagca tacaggaact gatcatcgct
    27181 aagaaataac tagcaactag ccaaacaaat ccttcaccaa tataaatcgg gaaagacaaa
    27241 atacttcttg tagcaactgc caaagcaccg gctcctgcgg caccagctac tccaccaaat
    27301 atagctccac atatacaagc aatgtaaaat ggtcgtttat atttaagagt cacaccataa
    27361 acagcaggtt cagtgattcc aaataatgct gtgataaatg cagataaaga taaacttttt
    27421 actttcttat ttttcgtttt caaaaatacc ccaagtactg ctcctgcttg cgcagctacg
    27481 gcagtcatac atgctggacc tagcgtatca cgaccgtaga aagaaagatt gttggtcatt
    27541 actggtacaa gtccccaatg taacccaaaa atcactaaca cttgccaaaa tccgccgata
    27601 acagcaccag ataaaacagg gctaaagtta taaacagcta aatatccagc tgcaatcagc
    27661 tgacttgcat aatctgcaat tggacctaca actaataaaa taattgggac catcacgatg
    27721 atacaaatga atggtgtcaa taaatttcga atactttcgt gcaagctttt gcgaatctta
    27781 ggctcaatat aagaaagaac ccatacagct aaaattgctg gaataaccgt acttgtatac
    27841 cttgctagaa tcacgggcat ccctagaaat cgcaaggtta tgctatcact atatgcactg
    27901 acaatcgtag gataaacgag agctgctgca ataaccatag cgatgaaacg atctgtattg
    27961 aacttttttg cagcagtata tgccaaaaat ataggcaaga aatagaaaaa tgcatcagct
    28021 gctgcattca aaatgatata agttcctgat tcagtagtta ataaattcaa actcgtacat
    28081 aaggcgagta ctcctttgat caaaccactt cctgccaaga cgcctaaagt cggggcaaaa
    28141 acagcggcga tcatgtctat aaaagagttt aaaatgcctt ttttctcagt tcctctcttt
    28201 ccactagatg catcatcttc tagtttcatt tgtttggata atacgtcata cacttcattt
    28261 actgcatttc ctactacgac ttgaaacatt cctccagctt cgactacagt gatcacacct
    28321 tctagttgct ctaccttctc cttatctgcc ttcttattat cttttagggt aaagcgtaac
    28381 cttgttgcac aatggtaaac attgctgaca ttttcttcgc ctccgacaaa tcggacaata
    28441 tctttcgcta attgtgaata gtccattttt acttccccct tgttaattaa tgccgtagca
    28501 atccttctta caagataagt atagttctta gaaaacggtt tctccattca attagtaccc
    28561 cttctaatag cgaacgaaac tgtacaatag tgtgtatggt ttacttagcc gagcaaatca
    28621 ctcactgctt gaaagaaaac aagtatatcg tttctcttat agtcaaagga gacaaaaaaa
    28681 acacgcttga tcttcctttg ctgatgtata agtggtatct caagctgata ctctgtgatt
    28741 tttgtttcta cttggctttt gataagacag cacaacaaag tctcggcatt taacggaagc
    28801 ataactaggt cagaactagg agagattagt tgctgaatgg aatctatctt tttatggatt
    28861 tttatttgat tttcttcttt gtagtaatga tcaagcagtt ttgttttctt tttttcttca
    28921 actatggtca gcttttcacg caaaatctga taaatctccc aagattcaaa ctcatctaga
    28981 acactaggta gataaatcaa atcatcccac tcaaaaataa gctcttgctc agtagtcaaa
    29041 attgcatcat attccgttat tttgtttgat tgatttttga aaagatacac tggttctatc
    29101 cggcattctt tgatccacat gttcatactt tgttgaatct gtttttttag tgtattcaaa
    29161 atactaattg gctggttact aacaatcagt atctcccctt gttttcgaaa aggattcaac
    29221 gcttcggcaa gatacaaaac aaaataagcg ctttcagcta aattgggacg taattgaaaa
    29281 tgtattggga agaaacgccg aattaaataa atttctaaag gataacggga gatgatcttt
    29341 tgtacaaaat gattcatggt attatgtccg gcatccatcc tcgttttcat ttgtttgata
    29401 tgggttagca aaaattctgt ttcatggaac aataacgaaa caggaagatc taaaaaacga
    29461 ataatcgttt gctcaaatgc tcttagattc ttctctattt ctttgttttt ttcatcatta
    29521 atcatcaaat aatttaactc taatagtcga ttttcaattg cttgtttttc ttgattggaa
    29581 aaagaatagg gtagttctga tgataacttt tttgtgaggg tctctaccat cggaataaat
    29641 aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc gaagaatggt caaaacgaga
    29701 taccgagaaa atcgtatgaa gtcctctcct gaaacaatat aattatatga tataagaacc
    29761 tcttttaatg tcttttggac agtcggcatg atattggaaa aaattgtact gatttgttga
    29821 ggaaaccttg cgtgttggat caccgtttct gttccaacaa gagataagaa aattcttttt
    29881 gaattttctt cagcaatgat ttttagtcct tttgtcgacg aaacttccag ttctaattta
    29941 ggattgcgct cgatccaacg caaaatcgtt tttatttgta atgatacggc tgtttttgaa
    30001 agaaagaaaa tgtctgctat cttttgcata ctgacatacc ctttttgaaa caataagtac
    30061 gtcacaatag ttgaagctcg gaaatccatg ctggaataga ttttatgttt cgtcacctct
    30121 tgtatgattt gctgtttagt ttcagattca attgattcta aaaaataacc tgaacgttta
    30181 tttgactgga taatgatttt cttctcgtag atagcaagca tatttttgat ttcagtttgg
    30241 atcgttttct tgttggtacc taaagcttca gctaattctt cgcttgttat ccaatgattc
    30301 ttatttctta atactacaag aatttggagt tgtcgtttat ttaagtccat ttgcttacac
    30361 ctcctctttg ttataaacat acaggaaaaa cctctcaaat gacaatagtc acttgcagag
    30421 gtttaaaaat catttgaaga aagatctata ttcaaggttt ttaccattag actggataat
    30481 ttgtttataa tattcaaaac tatctttttt caatctccta aagcttccgc tcccatcatt
    30541 ttgtctatcc acataaataa agccatatcg cttcctcatt tcccctgttc ctgccgatac
    30601 gatatcgatt ggtccccacc ataagtaacc aatgatttgg caaccatctt ttattgcctc
    30661 agcaatttcc tgaagatgta tttttagata ctcacaacgt tttggatcgt ggattcgatt
    30721 caattcatct ggtgcttcat ctagtccaat gccattttca actatgaata atggtaattc
    30781 ataacggtct gtcaattcat tcatgacata acgaaaccct agaggatcaa ttggccaact
    30841 ccaaggttcg ggagattttt ccttcaaaaa gggattatcg aaaccatttg ctccgccagt
    30901 atctacgcgc atttctgctt cttttttata gacagcgcta cgataataac taaatgcaat
    30961 ataatctgcc gtgttagctt gcaagcactg ttcgtctcca tctaacatta ctggtgcaca
    31021 gtcttcttcc ttccatgtcc tgtaaatata agctggataa tgccctcggc acatgacatc
    31081 ggagaaaaac catgaattgc gtttaaattg cagtgaacca aatacattct ctggatcaca
    31141 gtcatatgca taagtcgcta atgcagaaaa gctacacata atacccatct tagcatttgg
    31201 atcgattttc tttaataatt gagtagcacg ggcgttcgct agaaacatat gatgacaggc
    31261 ttgataaatc actttttttg ttagtgtagc aatgggttca agtttatctc tagcagggaa
    31321 tgtaaccaat ccagcagctg cataaggcat acgaaaagca ttattgatct cattaaaggt
    31381 catccaatat ttcactttcc cttggtagcg ttcaaagacg gtctgcgcat aaaattcaaa
    31441 aaagtcgatt aattttcgat tttgccaccc cccatactct cctaccaatg ctaagggggt
    31501 ttcataatga ctaagagtaa ttactggctc gatatcatat cgatttaatg tattgattag
    31561 tctgtcataa aatacgagac cagcctcatt tggcttctgt tcgtctcctc ttggaaatat
    31621 tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct aactccttca gtaaacgaat
    31681 atcctcttca aatcgatgat aaaaatcaat cgcttcatga gaaagataat ggagtgattc
    31741 gtctaaagca atctcccaaa tctttttttc ttcattccac tgaatactag gatgctggtt
    31801 catgatcccg ataagcgtat ctgtcacatt aggtaatttg ccgtcttcca accatgcacc
    31861 ttcacattga ttagcagcta ctgctccgcc ccataaaaaa ttttctggaa atatttcctg
    31921 atccataact atctctcctc gctatcaaca atttcttact tgaagtatag ctcgattcat
    31981 caaaacaaga tattctaact atttcggcag aagagaagaa aaaaattgaa tgctactctt
    32041 catttttaat atttatatat aacaaataca taagacgatc cccgaaagaa aacactcgtt
    32101 aaccatctaa gcaaacgatt tattttactt ccaacacaaa tgcttcataa ggtcgtaaat
    32161 caactttgcc gtctggtaac tgtttcagag gataatttga cacaataacc cttaaagctt
    32221 cacgtggaat agtgaacgtc tgcttttcac tggacatatt acaaataacg agccattgct
    32281 ggtcttcata tatgcgagaa taggcaaaga catgctcagg cgtcttttcc actaattcat
    32341 agtctcccca gacgatgatg tcatgttttc tacgcaaatc gatcaatttc ttgtacatat
    32401 aaaaaacaga atcttgatct gctaacgctt cttctgcatt gatttcttta taatttggat
    32461 tgactcgaag ccatggaatt ccagtagtaa acccagcatg ttcacgggcg ttccattgca
    32521 taggcgtcct agcattgtct cttcctttcg ccttgattga ttccaagatt tcctcttttg
    32581 taaatccaga agaaatcctt tcgttataca tattgattgt ctcaatatct tttgcttccg
    32641 cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc tccttgatag atataagggg
    32701 tgcctttcat catatggagc aagatagcaa acatcttggc actttctttt cgatacacat
    32761 cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg attattccaa aataaactat
    32821 tccatccttc atcacctaaa gatgtttgcc atttggataa tacttttttc agttcagaga
    32881 tactcaaggg tttcaaatcc catttgtctt tgccttcttg ctgatctaat cctatgtgtt
    32941 cgaattggaa gaccatggat aattcatttc ttttaggatt ggaataaagt ttagcgattt
    33001 cggtagttgc tccccatgtt tcgccaacag tcatcagatt tttgtcacca aaagttgctt
    33061 gattcatttc ctgaagatac tcatgcagtt taggtccatt acttgtgatt tctttatccg
    33121 gttctttgcc aatcatatcg atcacgtcca tacgaaagcc cccaatacct ttatcgatcc
    33181 agaaattcat catttgataa atctcttggc gaacctgttt atttttccaa ttcaaatcag
    33241 gttgacgctt gctatacaag tgcagaaaat attgccccgt tttttcatcc aactgccaag
    33301 cagaaccact gaatattgag cgcaatctat taggctcttt tccattgacc ggatcacgcc
    33361 aaatataata gtcccgataa ggattttctt tatttttacg ggcctcaatg aaccacggat
    33421 gttcatcaga agtatgattc accaccagat ccatgatgat acggatattt cttttctttg
    33481 cttcttcaat cagttcttcc atttcttcca tcgtaccgaa ttctgccatg attgcttgat
    33541 aattactgat atcataccca ttgtcatcat tcggagactc gtatactgga gaaagccaaa
    33601 ttgcatcaat acctaaatac gccagataat ccaacctgga aataatcccc cgcaaatctc
    33661 caataccatc ttgatttgaa tcttgaaaac ttcttggata gatttgataa acaactgaat
    33721 ggtgccacca tttttgttcc atgattttcc tcctataaat ggatgtttct attttttgct
    33781 taatcattta ttttagtctc tctcactaaa aattgtaacg ctttcaaata gctattgcac
    33841 cttgttatcg gtaacagaat aaaacaaaaa aacagctgag gaaattccct caactgtttt
    33901 ttgaaaggat tgatttatgc agggaagaat gctgatttca caacttcttt ataaacggtg
    33961 ttcactcagc tgtcccttgg tattagctca aaaaacggca aaatataaga aactattttt
    34021 tcgttttttt ctctaatact caggccagaa cgctgatttc acaacctctt tatttcacat
    34081 tcaatatcca catgaatccc acaaatacaa agaacagacc atacatgatt ggagatactt
    34141 cttttcctct tttagctgct aacatggtaa ttggatagaa gataaaacct aatgcaatcc
    34201 catctgaaat actataagtc aaaggcatgc cgatcaagat caagaatgaa gggatcgcaa
    34261 tctctaagtt tccccattcg atttgtcgca aagattgtgc catcaatact cctaccataa
    34321 tcaatgctgg tgcagttact tgtgaagtaa caactgatag taatggagaa aagaacaagc
    34381 ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc tgaacgtcct ccaacggcga
    34441 taccagctga tgattctaca aatgcaccaa caggagaagt accaaataag gaacccgcaa
    34501 gcatagctgt tgaatcagaa gctaaagctt tccctacacg aggcatcttg ttgtctttca
    34561 tgaaacctgc ttggtttgct aatccaacta aagttcctgc tgtatcaaag aaagtcacaa
    34621 gcaagaatgt cagcactacg acccacattt gaagagaatt gatgtcccct acatgtttta
    34681 atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc aacaatttca gaaggcattg
    34741 gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc catcccgatg aagatgccac
    34801 ctggtacacg ccgaacaagc aagatagcag ttacgactaa tccaaaaatc gtcaaccaag
    34861 tagaaccaac gttgagtgat cctaatccta ctaatgtaga ttcattcgaa acgatgatcc
    34921 caccttcgct cagaccaagg aaagcgataa atagaccgat accgccagaa atcgcatatt
    34981 tcaaatctgc tgggatcgca tcaatgatca tttcccgcag cttgaagatt gtgatcaaaa
    35041 taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt ttcccatgga atccccattc
    35101 ctaaacaaac agaataagca aaaaaagcat tgatcccaag agcaggtgcc gttccaattg
    35161 gataacgggc taataccccc attaaaacac agccgatcgc actggctaat gcagtcgcag
    35221 taaacactgc tccttcatcc attccagaag cacctaatac agttggattt acaaataaga
    35281 tataagccat tgagataaat gttgtaaatc ctgccagtac ctcacgtttt accgtggtgt
    35341 tcaatttttc tatttcgaag taggaaagta ttttttcctt catcttccat ccccctaaat
    35401 ccgaacatta taaataaagt ccgttaaaat ttcgatttat taataaaaca acgtgtttta
    35461 tcatatacta aaacaaaaca taagtaaaca aaaaattcgg attttaagca aaatccgaac
    35521 gttccatctg attaaatccc tttatctcaa agttcatgaa cattcacttt ggattacaag
    35581 aatttcttta ttgtgttaat aaagtttctt cattctagtc tcaaaaatca atagtgtgat
    35641 ttgattttac catttcgttt ctgttcttct ttgatgaatt cttttgtatc gacaggattc
    35701 agattattta gtccgccaaa acctgtatcg accatttttc cccagccttt tgtcttcttt
    35761 tttcctgatt tgagattctt tgctcgaagc ttctcactat gttttgctcg ccgatacttc
    35821 aaataacctt ctaatcttcc cattagatcc actgtttttc ctccttttta ttcaatcaga
    35881 cgactcagct tttcctataa caaaataatt gccttcaggg tcagaaaaat tgaaagtcaa
    35941 gatgccattg ttatctgtga tttctcctgg ttcttccatg ttctcgtaga tagtttcaaa
    36001 ttcatccgta aaaaatacta aagaaggcgg tggtcctaat acctctggtg aaaacttctg
    36061 cacaaatact tttgggaata cgcctaattc taattctttt gagatgctga gtacaatact
    36121 cttaaattct tctggcaatt cgattgtttc agaaatttct gcaccaattt tttctacaaa
    36181 gaatcggcaa atcatctcta cgtcattgac ataaagcatc acacgtgttt tttgtatcat
    36241 catttgaccc ttctttctat ttgattttct ttcctgtcta taaactgatt ccattatatc
    36301 acgaaagcgc aaacaccttg tgtttgatac tattcaagaa aaataaagca atagtttcta
    36361 ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa cggcaatgaa tgccgctctt
    36421 cttatttcga ttcttttata attctgcttc tgattcatct ttggttagtg tgtgtctaac
    36481 actagttacg ccaaatgttt gatcgatttt ttccttcaac gcttcgattg cctgtgcgcc
    36541 acgtgccgct tgttcagcat tgatggattc aatgaccagc accgctgctt gtgtttcttc
    36601 cgtcagcatc gttctttggg cttcacgcca gttgagacag cgacagatcg ctccttgttc
    36661 atcatagtag atgatttctt ccggaagtgc cggagcatcg gaatctgcac ctaaaggcag
    36721 gaatgattcg ccaccttggg cttttcctaa atgcagattt cctgcgatct tttcgatatc
    36781 ttctccgccg caaggtacgc caaaagacaa ggaaatacta ttatagatat ctacaagcgg
    36841 attgattggg aaaaattccc gttcttgatt tgcacgtttc agtaaagctt caatagaaga
    36901 acgtgcacct tttttagtct tgaatgtggt aaatgcctga cgccactgag caatgacttc
    36961 gttttggctg aatgtttctt ctgtaaggta atttcttgct tcttttgctg cctcattcaa
    37021 taattgatga tacgtttctt cactttcttt tgttacgtgg ttatcgatac catttacgga
    37081 taagatttcg atggttgctt caggaaatac ttcccagaaa ttggtatcca ctattacttt
    37141 tttcatctgt tcacacctct tgattagaat aattgcttga tgaatagata gtctgtttgg
    37201 ggatcatctc ccatataaaa aacatgttcg ctgtatttga taaatccttt tttacgataa
    37261 aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc aaatggctgt tttttgcttt
    37321 tcttttgctt tttcaatagc tgtatctagt aaaaatgtac cgataccttg atgtttaaat
    37381 ttttttcgta catagattcg ctcgatttct aaaaaatgat cagccatttc ctcagattga
    37441 ctcgttcctg tatttagttt caggtaaccc gcaggctctt gatcgatact cactaggaaa
    37501 aaatcggttt ctttatcttt tagctcttct gtaagttttt ctagtgcata agcttgtgtc
    37561 aagtaatcag ctaaatgctc cggtgaatta tcttttccaa atgtatctgt aaatgtttcg
    37621 atactaattt gttgtagtaa gtctacatct gaaggcgtca tttttcgaat ttcgtattta
    37681 ttcacggctt cttcctttca atagatcctt ttattgcctt ttttgacaaa attccaatct
    37741 tcgctaacat tcttgcatac tttatttaaa agatattcca attgttttgc ttcttcgtca
    37801 gaaagaccat ttaatgcgac tgcattggag tagttgtttt ctcgaatgat gaaaggatag
    37861 atttctgctc cttttttagt cggatagagt ttcttgattt ttttattttc tttatcttcc
    37921 aaacgttcga tcatcccatt ggattctaat ttcttgattg ctcgggcagt cgttgtacga
    37981 tctactttga tcatttctgc tagtttttct tggatgattc caggattttc gcaaatacgt
    38041 actaaataaa gatactgtcc tcttgttaaa tcgacttctt tgaattcgat attgctaata
    38101 gaatctagcg ctcgggcaat cattccgatg tctcgaagga tttcagccat ttcatcccac
    38161 ctttattttg tttgtaagac tagttcacct ctttttgttg caaatgcaac tattttcaat
    38221 aaaaaaatca agttcttttt taataactag taaagtatat tcttttcttc ttttacgaaa
    38281 aagctcaatc acaatagatc tggtttttgt tttctaaaat aagtaactgt ttcttcgttg
    38341 ttcgcttttc tgatagcttt ggcaactctt ctatactaaa aaatcccatg ttggaagttt
    38401 cattattctc aataaacttg gcctcttcac cgtggatcgc acaagcaaaa acaagtttat
    38461 aatactgaaa aagctgtgga atatcttttt gtttattcgt atcaaaaact gctcgcaact
    38521 gagtagtttc tactgttaat cctgtttctt catagacttc tttacgcaca ttttcttcag
    38581 gagacaatcc tatttcagca aatccaccag gaagtgacca ttctttcgtc cgtttatctt
    38641 cgattagtag gaccttgcca tcttttttga tataggcacg aacatctact tttggtgttg
    38701 gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc gaaagtttct tttcctattt
    38761 cactgatcag ttccaatgaa atcgtccgga tctcctcaaa acgctcttta tcaaatacat
    38821 cttttccata atatagacca gcttgagaaa gtgcgagtag tcttttgtat tgagccaact
    38881 tcttgttcat tctcttccct tcttttttag caaatgtatg actatgtaat caagtataga
    38941 agactggttt aattacggca aataaatata atgaaatctg caataaataa aagaatgtta
    39001 aaaaactcga gaacaaaaga cattcgactt ctctggggct gtgaataaat ggcgttcaaa
    39061 agcttgcatc tgcggtaata agccccacca aacgaaaaat atgacgagct attcttgtct
    39121 ggctgttctt attaattgag gcaggacagc tttttcacaa cctccccact aaacggtgtt
    39181 ctcctaccaa ctcctcggtc acaagtcaca atattcatca aacatgagaa gctgtttttt
    39241 tgaaattgct ccttgtgact cggagctgaa cgacaggctc acgacctctc ggtaaacagc
    39301 gttcaaaagc caatttttcg atattaagcc atttctttgt tacttattgt cattgatttc
    39361 tttttctaat ttatccaata gtggagtgta atccacttga gtatagtctg ttacatcaaa
    39421 ttcatatagt gttcctaact ggaatttgtc atattttcgg tcggcgatga tctttttgaa
    39481 tgcttctttc gtaatatgat tggtcccttt cgttcgatct tccaatacgt caccatagtg
    39541 ataatgatcc atgatatagc tcaaatgacg atctgattca cgatcacgtt tgtaccgacg
    39601 ttgccataac acttcaaagt ctgctgttag gcgaatcgtg atcacctgat atccgtatct
    39661 atctgctaat tcttggagtc gtgctttttg cttatcacta aaaggatact cggacacaat
    39721 cactttcttt cctgcttcca tatacaaatc aagtgctttg taatagaaag gataaacttt
    39781 cgtcctttct tgttccgccc gttcttccaa attgttaaac ccgatggatt ctgcatagta
    39841 ttccttgatt tcatcgagcg tgatggcata catatctgga aatcgttccc gtagtttatt
    39901 cataagatat gtttttcctg taccaggact accagccaga agaatgaaat actttttcat
    39961 ctcatttatt tcctaagtta attggacttt ccaagttcat aaacagtcca atcttatgct
    40021 tgacaatttc ttttactgct tcatttgctg gaatcacatt atggcgaaga gacttgttga
    40081 tttctgtttc actaaaggtt tctttagcta ctttcgtcca gttgactaaa agctccgttc
    40141 caacattgaa tttgcggatc ccgttattga tcaattcagg ataatcttct tctttcacac
    40201 ctgttcctcc atggataact agggggacat caactactgc atcgatttct ttcagcaagg
    40261 gaatgtttac ttctgttttt gatttgaatt gtccatgatt cgtaccgata gcaatagcta
    40321 atgcatccac gcctgtagct tctacaaatt tcactgcgtc ttccggtctt gtatatacct
    40381 tgtcattttc agcgacatgg attccttctt ctgtaccgcc gattgttcca agttctcctt
    40441 ctacggaaac accgtgcgca tgggcatatt ctactactgc ttttgtcttc aggatatttt
    40501 ctttgaaagg cagatgggac ccatcataca tgacagaagt atatccgctg tcgattgctt
    40561 ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa gactgcatca accatttctt
    40621 cttctgcaat agctgtcatt acattgacta acacatgcat gccgatatat ttagcggtat
    40681 cgacacttgt ttgaataatg atcggcgaac ccatttcttt tgctgcgcct agcatagcag
    40741 gaagcatttc taggttatgc gtattaaaag ctccaactgt aaaattcaac tcttctgctg
    40801 tttttgttac ttctttcaat gttgtataca tcttttattg ccaccttatc tttttatttg
    40861 tttcctttga tcgttacttg ttttgcaatt tgtcgcatct catccatttt gcccatgtag
    40921 taatcgatcc cttgatcatc accagcacga gcagcatctg cacgcttctg gttatagagg
    40981 ctcatcagtt ttttataccc atctccgatc gtctgtttat tttccaaact cttttctaaa
    41041 tattggatgg cttgatcgtt cacatacaat tcagaaaatt taagtccaac ttgttcataa
    41101 agtttcgcta atttatgctt atcttgggta gtggaaatct cttgtttcaa ttgtgtaatc
    41161 tctgattgaa tcttttcggt cgcttctggc gatagggctt tttcttcttt tgccagaact
    41221 tttttcggtt ctttttcttt tttcttaaaa aatccaaaca tcattttccc tactttcaat
    41281 taaaatgctt tcaaaactgg gcttaagatc caggcatata agagtgctgc ggctactgta
    41341 tctacatcgg ttgctgtagc attgttgaat cccatcgaat taaagatcgt tactagcaaa
    41401 gcgggcaata atgtaataaa gaaaccatga gcaataccgc caatgatcgc tcctcggcgt
    41461 ccgccaactg cgttgccaaa tatccctgct gtccctccgg caaagaaatt ggttagcata
    41521 ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa acatggcaat aatcgtacca
    41581 atcgttgtcg taatgaaacc taaaatgaca gcatttgggc tataaggaaa gaaaactgga
    41641 caatctaagg cagggatcgc atcaggaact aatttcatag ctatccctcg aaatgccggc
    41701 acgatttcac ctaacagtag acgtacacca gctagcaata catacacacc cactacgaat
    41761 tggatggctt gtaaaaaggc aaacatgata tagttttgat ctcctgataa ttcagaagcg
    41821 aatggttcgc ctgcaaataa aacagtgatg atatatagcg ggaccatcac gaccatgaca
    41881 gacaaataag tatcttgcag aaattcgaaa tgtgtcggca acttgatatc ttcgatcgac
    41941 tttttatttt cgcctttctc accaaaaagc ttagcaacac cggcttcaaa taagtacccg
    42001 atcgtacaaa aatgacctaa agcaatatca tttgatccag tgattttacg aatgatcggt
    42061 tgcgctactg ccggcatagc aatggcgaaa caagctccga taaaaccacc gaccaagatc
    42121 aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg tcgtcatcgt tgccatccat
    42181 aaaattgctt gaccagttaa aaaaatgtac ttccattttg tgaaacgagc caaaataata
    42241 ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc tacctaaacc caaatcattc
    42301 attgcttgac cattgataga ttcaatagaa gggatgatcc cttgcatatg aaatccttca
    42361 gtaaagattt tcccgaaata agttaaactt ccaacgatga tgctcgaacc ggcactcaac
    42421 acttgaaatc ctaggagtgt ctttaacgtg ccagagatga cttgtcctgc tgatttcttc
    42481 tgcagtagta gacccaacat agcaatcaat gcaatggtga tcgatgcctg agtcaaaata
    42541 ttttcgatga taaaattgat aacgcccatg tttttaccct cttttctttt tgtatttaga
    42601 taactccctt atcttgcaga actggggtga ttttttcttc aatttcattt tttgaaacaa
    42661 tatttttcaa atagatcatc gccgttttag gatcaactgc gaacttttcg aactgcgaac
    42721 gaaaattttc tgctgtgatg atcacatctg gtttcatgga aactgcggaa gaaatatcac
    42781 aatggtccag tttggcttct actcctaatt tgttcaaaac atcctcagca gacatctgtg
    42841 ctgcaaaact acttcctaaa cctgcaccgc atacgaataa aatgttcaat ttgttcatct
    42901 ttgttctccc ccgtatcatt tattatttct caactactga aaaaagcagt tgtttgaaat
    42961 cttcgatatt tgtgcattca atcaatcgat ccaacttctc ttcgttgttg atcaattcaa
    43021 ttagttcttt catgatgttc agatgcgagt aggagtctac tgctgccaaa caaaaaataa
    43081 tctttactgg atccatttca ggattaccga aatcgattgg ttgctcaatc gtagctacgc
    43141 tgactcctaa tttattgacc ccatcttctg gtcgggcatg ggctagtgcc atgtgtttgc
    43201 caatcacgat atacggtccg taatcattca ctgcacgaat catcgcttcg acatagcgct
    43261 cttcgacaat gttttcttta accaatggtc tagcagctag ttcaatcgat tcctcccaag
    43321 tagtggcttt ttctttgatc atgatattgc tgtccgtcaa aatatctttt aacataggct
    43381 ggatctccct tttgttgatt tctaaattgt ttgtttcgaa taatttttct aacttgccat
    43441 agatgattcg atctactcgg ccaccacttt ctttcatgac atctattatt tgagtgaata
    43501 actccgtcga ttcatgactg ttaggctcta accgctgtag ttcttcattt cttttcaaaa
    43561 agtcttctat aaccaagata ttcgtctcag ttaaaatcgg atcgacaact aaaagcggct
    43621 tatttcgata attcaattga aaggtgctga aaaccagatt gacatccaat ttatctacta
    43681 attcgatttc ttttgaactt aaaactgcag taatctcgat ctgaggaaac ttttctttca
    43741 gattctctgc taacagattt ccagtagcca tcccatggtt acagacaacg actgatttat
    43801 aaacataatt caagtctcgc ttgattacac tggcgatcgt cgaaaagtgg atgacaagaa
    43861 aagctatctc gtcctccagt atcttgccac caattacttc ttcgatcgta ggaacaaaat
    43921 gagtgactgc gcggtagatt gggaaataat ttgttcggat gttttctttc aatggattca
    43981 tgacttggat atgattcttg attcttacaa tcagtgcagc catgtgctta tagagatttt
    44041 cgaataaagc ttcttccttt agatggaaag gtacgtgtgt ctcgttctct acaaattgga
    44101 tcaattggat cgttagtaat tgagcttgta cccactcgat tgaattggag atatctctag
    44161 cattgaacgt ctcaatagtg aaacgaagat aatttctttc tacttgcggg acattgtcca
    44221 attgaaattc ctcgatcatg tggtcaataa atgcagaaaa gctactgtct tttatttctg
    44281 tatttttcca attaatggca gagataaatt catgtttcct gattcgctgg atccagatca
    44341 atgtaaaaag caggatttgt tttttataaa tatcatcttc ttgtttcaac aacttattgg
    44401 aatagatctc ctgtattttt ttgatttcca ccaaaggaaa ataatggaga aagatttttt
    44461 gagccggtgt taattgtgca tcttgattca taaagaagtc ggtcattccc agattctgat
    44521 tgataaaatc atagatcatc gtacggatag atcgttctga acctttataa accagtcctt
    44581 gctttccata actgacgatc tctatcccat aactgaccag tgttgctcgt aaccgtctca
    44641 tatcttcatc taatgtactt ttagagattt gaaattcttc ttcttttttg tttagatgga
    44701 tcgggattcg gctgtaagca acggctaaaa ctagatcaaa aatacgttcg cgccgattca
    44761 aaaagccgtc tttcacatct tttatttcag tttctaatcg agcttgcttt tccttttcca
    44821 gcttcaacaa aaaacctttg ttgcgaattg tacggatctc cggaagcttt ttactgctca
    44881 accagatatt cgcttcaaga acttcctgac ggatcgtccg ctgactgaca ttgaactcac
    44941 ttgctagatg ttttgctttt actggatatt gggctgtcat gattctgtga agtatttcaa
    45001 ttgttcttgt tttcatagaa atgatctcct tctgatttca agtatatggt tccgcttaca
    45061 taataaaaag tagatatttc tgccacttga agtggaaaga aagtaagcaa agcaaacaaa
    45121 aaataagcca tatgcttttt catctcctca acgaaaaaga catacgccta ttattacaaa
    45181 aattttgccc atctatacaa aaaagaggtt gtgaaaaagc tgtcctgcat caagtaataa
    45241 gaacaactaa actaaaatag tgcttcctat ttttcgtttg gttgttctta ttatcgcaga
    45301 tgcaagcttt taaacaccat ttagtggaga ggttgtgaca gagatgttca gccttgagcg
    45361 ataagaagcg attttgaaaa atcgcttccc atattttaca aaatcgtgcc ttattgccga
    45421 taggctatct ctggcacacc gtttattcag aaaaaggagc tgcgacaagg cttttgtcac
    45481 aactccgttc ataaatattt cctgtcaatc cagcataatc ggctgaataa aatcggcttg
    45541 ccatcttttt tctttctcag aaaaatcaaa ataccatatc tgagaaaaat actgtcgttg
    45601 tgtcttactt cctgagatca atcgcccatc ttttcgatcg actgaataat cacagcatct
    45661 gaaatcaatt cggatggcaa aactattttc gtggatctgt cgataatttc ctacttcctg
    45721 aatagttatt tttttagtat gatttcttac accgtctgct ttgttttttt gcagaacttt
    45781 ctgatgttcg gcaaacagct tgtctgtata acagcttctg gctttttcag gagtttcttc
    45841 ttcccaagcg gattggatct tcatgaatat cttttggatt tccagaaact gtgctttctt
    45901 ttctttagcc gttcctgaca tgcgggacaa tatctccgct ttcagccttt tcttttgtct
    45961 tttattcttg acgatagaaa aagcagaaac ccctcctact gacaaaacag ctaatgcaag
    46021 aaatcccgtt tgctcataac tagaaggacc tgtacctgga ccataatacc cataagaatc
    46081 tctgccgtaa gaacgactgc caaaataact gcccccatat gaacgaccac caaatgaacc
    46141 tctgcccgaa cttcgaccgc tagatgtacc tccaccatga ccacccgcga cagcgaatcc
    46201 ttgggtcggc agagcaaaca aacataggcc cactaacaaa atgattgcta aataattgat
    46261 ttttttcatt gtcttcctcg ttatctttta ttacaaaaaa ctatttttat gatagccttg
    46321 ctaaaaggaa gagtcaataa ccagatgcat aaactgtttt tttagcaatt ttgtcttatt
    46381 tagtcattga gtaacatcgg acaaatgtat gtgtcggcag tccataattc ctctatcttc
    46441 agcatgaaca agcttgcaga caagagatct gcgcttcctc caggagagat ttggttttcc
    46501 tgacaaaagt gcaacagtgc tgtaaatgga cgatttggta aagcactttg actagcagca
    46561 tgagcaagtt tttgcatttc cactaacgtt ttctgatcac tgcgatgcaa tacgcatgtg
    46621 tcatctagcg aactcatcaa aactaacaac atatgcattt gtttctgtac atatgaatca
    46681 ttatctagct ccttagctgc ttgaattgca agcctgatat gcggataccc taatatcgct
    46741 tcttcatatg caccatttac gccatatttt tgcttcaccc tatgaccatg agtggttttt
    46801 ttatgacgat attttgtatc aggaaaagca gcaagcttgc tgacatcatc aagtattttc
    46861 gataaagaaa aaggctgttc catactgatt ttttgtgcaa tgacactgat caatagcccc
    46921 attgcccaaa tcgctccttt atgggtattg atgccatttg tagcaatcag cattgccttt
    46981 tccgcttttc gcccaatctc agcaattctt tctcttaact cctgatcaat agcatgatgc
    47041 catgctattt gagccatttc ttcaaaaaaa ggagttagcg tcttagcaga cttaagaaat
    47101 aaagcatagt ccatatcgga atgtgcaccc gcatccagtg catccactaa tccaggtttt
    47161 ggacttaatg ttacttctgc gatcaatgct tcctctacca acgcagcgag ttgctgagtc
    47221 actgcttttt ttcgttcgtc atttatcatg acactcactc cttcttccta ctattacttg
    47281 ctgaaacctt ttttgatagc tacccaaatg atatgaagta accagcttaa agcaaatgca
    47341 caacttgccg tcattagata agctacaaga atcatgactc ctgaaggtaa ctgcagccaa
    47401 ctatctttaa acaataataa cagtacttgg aaaacaaata cattggctaa atacgtacga
    47461 taagcataaa cagacaacca tttgaacaat ggcaattttg gcgaatcgtt tacaatcatt
    47521 ttggaagcgc ctatgaagac agcaaagatg atgactaaac tgtataaact ttggatcgta
    47581 ttcaagtaag gtgcattcgc aaagctcaag tctcctggat gtgcgagcaa tgaacggaca
    47641 gaaaagaacg cgataactaa ggcaacaggt aagaaaagat aacgtactct gtccaaatat
    47701 tgatagactt tttcgtgata aacaaatgcg gctaccccat aaatcatgaa actagcaaca
    47761 aaccgatcta ataagtacca gctgtcatga taaggcccag tcaatacttg tgtttgatag
    47821 aacagatacc atgcgacata taaaatcgtt gaaccaatca atactggcca tacatatttt
    47881 ttattagcaa atactttgta accgagccaa acaaaaaacg gcatgaacaa ttggatctgc
    47941 agcatcataa cggtatacca taaatgaggc gctccatcac ctgtgacata tttcagtaaa
    48001 aagcttgcag tatccgtata cggagtttgt tgttggactg ctggaaatag ccaaagataa
    48061 gccgtcgtcc atagaagata cggaacgact agttcaaaaa acttttcttt caaaaattcc
    48121 caataagaca aatgctgact tgtttttacc atattataaa caattgcaaa aataaacatt
    48181 ggtgctgtgt atttaacgat gacgtaaaag gtaccaagaa aagctgtatt tgttgcgctg
    48241 tggctgtttg ccaaagcaaa gctcaatatc gtttgcatca taacagccgt tacagctgat
    48301 actttctgga agtcacttac gttgattttg atttttttgt tttcaaacat ctctttctct
    48361 ctctttttct ttattaccaa agttctttgg ttaatttagg tccttcgact gttttcaata
    48421 gaattttttt ctctggctct ctcaaatact cttttagtga aaaaccgcct tgccgagtca
    48481 ctacttgggt atcgaattgc tcaaaaaaag caggatgcga ttgaattacc tgaattggca
    48541 attcaatcgg tgaatttgcg tacagcagca aatcaaaatc actggtttct tttactgccg
    48601 gaatcccagt cgataattca aatcctaagc tgccgccgat tccccattta catcctgata
    48661 atatgtttct agcagcatga aattgctgat aaacaggata atccatgatt ttctcttgtc
    48721 tatacgattc ttgttcgatg atgtcccacg gatgaatgac ttgtttgacg gcatgttttg
    48781 agatttctgt tgaccatctc tgattgcggg caggtccacg taaaccaatc ggtagtattt
    48841 ctgcgtgatc cgtcgacgca cgacgaactg ttccgaacat cttttcagtt agaacgaccc
    48901 attcaggcaa ggtgaaccct tctatttcat gtttatcaaa aaggacaata tcatgtgctt
    48961 gaatcatttt ttatgcatcc cattcttcgt ccattttggc acggacttta tttgtttcga
    49021 cacgtccgaa tgttttggct gtttcgtttg tgtatcgatt tgtcagcatc gtgtcatttg
    49081 tattgcggac atcttgaatg gcttcattga ttgcttcgat gactttgctg acattttgtt
    49141 ctgaagcagc ttggtcggtg atatcttcta ggaaatgata gatcgctcct agttttgtat
    49201 aattattgat atcgtaagcg atagctggaa ctttttcagc agctgcttcg atttcagcaa
    49261 ccgaacgttt tgtcacacgt gcagcacttt ctttagacat cgcttggacg gtaatttctt
    49321 tattgtttaa agcaatcaag cggcttgatt gtaatccatg tgctaaaaat gcacctgaaa
    49381 tagcatttcc tgcaatcaat ccaataactg gatgtccagc ttgacggagt tttgcataag
    49441 cagcagcact attagccaaa gccacatgga taccgatcaa ttcttcgtta tagccgtatg
    49501 cttggcttgg tacatcgatc acaacgatga ttggccgttt cacttttttg tctttatctt
    49561 cttcgtaaac gtgatccaaa atttgtccca tgcggaaacc ttcaactaaa ccaacttcac
    49621 cgtttcgtac acggtacatc gggtttttag gatcaggtac gattgatgtc acgatggctt
    49681 cttctatgaa tttacttgat ttaccataat acacagtact aatagaagaa gttggatttt
    49741 tcatgccagt tagtgccgca aaccaatcgt aaccaacact tgctgcagct ggttcatttc
    49801 cttctgctac ttctggtaag tcaattgatt ttgcttgatg gttagcaatt gcttcattgt
    49861 aatcgtcaat ggccattggt tttgaaagat ctaaagaatc aagtaatgat aaataaaatg
    49921 ctgagctttc actgcgatgc gtgtcttttc gttcgtccat tgctgcgatg acagcagctt
    49981 tgattgcttc tacactatca gtcactaatt catcaatgat ttttgattct acccgttgac
    50041 gagatcctaa tgtattccaa atcaagtctt tgtcactaga atcaaattct cgaacgcctg
    50101 cttcttgttc aataacttct ggtccattca aaccgactct cgcttttttt gttgcgatta
    50161 aataactcat taatgctgaa gtaatcgaca taccgccaaa tgatcctaca cgtcctggaa
    50221 tcaaaccgat gactggtaca tattgtttta gagcaatgat tacatttccg atttctgaaa
    50281 tagataacag tccgtagttt gcttcttgca aacgaacacc acctgtatct agtacaatga
    50341 ctggaaagat ctcatttcct tttttatttt cttctaacac atgttcaagt gcagcaacga
    50401 tttttgcacc ggaaacttca ccgataccgc caccttggaa ttttccttcc atagcgatta
    50461 ctactgcttg tttgccattg atcgtgccac ggctgacaac gatcccgtca tcactttcag
    50521 ggacgattcc ttgtgctggt aaacttggcg cgatgatttg atcgaatgga tcaatcaatt
    50581 cacgtccatt gtcaagtaga gcgttcactc gttctctggc gtttaattct acaaaactat
    50641 ttttcatctg aaagttcctc cattgcttgc gtcaatcgta ggtacacaac tcctggtgta
    50701 gcaccgaagt cattgatcac gatatctgct gaaattggat aacgatcaaa aaatctcgtt
    50761 aatacattgt tccatacttc gccgaagcca tcgcttcctg tacaaatttt tacagtagat
    50821 tctgtttttt ctgttggaaa catcaagatt tccaaatctc ctgaaccgac cactcctaca
    50881 tggattttct tattgatcgg ctgtttcgtt ggatatttat aagctaattg ttccatggat
    50941 ctctcccctt tttgtaaaac agagcataca cgttctgctt attcgtcttt cttcttccat
    51001 ctgggtaaat ggcgacaaaa ggtttttctc gccaatttta tgaccaagtt ctgaatttca
    51061 ttggtggttc atacaggcca tctgaccatt cgatcaaatc atcgatggtt tgtgcagcta
    51121 ataatgaacg tttcgcttca ctacgtttga tgcctagatc ttctggtaat ttcacgatgc
    51181 cggctgcacg caagctatct agtgattctt tcgtgctagt cattccaaga ggtgtaacac
    51241 cgccaattgc tgcaattgct gcttgtcgtt cttcttttgt ttttgctttg tacaaataag
    51301 caattccttc ttctgtcacg atatgtgtag tatcttcgct ataaatcatc actggtgcat
    51361 tggctaagcc tgcttgtttt tgaacagcta gtgcatctaa ttcttcgaca aagactggtt
    51421 ttttatttga accatatgtt tcaaccattt gaacaactaa tttttggcct ttccctaatg
    51481 gatcatcatt gtcgcgaagt gattgccatg ctggtgtaga atgacgacga ccgccaggat
    51541 tatgtcccat gttaggcgca ccgccaaaac cagataaacg tccttttgtt acggtagaag
    51601 agtttccttc gtaatctaat tgcaaggttg aaccgataaa catatcgatg gcatattgtc
    51661 ctgcagcttg tgataatgtt cggttactgc gcatcgtgcc gtctttaccg acaaaaaaga
    51721 catctgggcg agcagcaata tatttttcca taccaacttc gccaccaaaa ctatggatgc
    51781 tttcaaccca acctgattcg atggctggaa tcatcgttgg atgtgggttc aatacccagt
    51841 tcttcgcgat tttccctttt aaccctagtg attcaccata agttggcagt aacaattcga
    51901 tagctgctgt attgaatcca ataccgtgat tcaaagattg tacgccgtgt tctgcgtaga
    51961 ttcctttgat tgccatcatt gccattagaa tttgcaattc tgtgatgttt tgaggatctc
    52021 gtgtgaataa agcttctaat tgataaggtt tatctgcttt cacgacaacg tctacccagt
    52081 caccagggat atctacacgt ggtaaagaat cgacaacttc gttaacttga acgatcacga
    52141 taccatcttt gaaagctgca gcttcgatga ttgttggtgt ttcttccgta ttgaaacctg
    52201 tgtacaagtt gccgtcttta tctgctttat ctgcagctac taaaacaaca tttggaatca
    52261 aatcgatgaa taagcgtcca taaagttcca aataagtatg gatatcacct aatttcattg
    52321 tgccgtcttc aatcatttgg gcgacacgca agctttgtgc accagcataa gagaaatcga
    52381 tcttgcttgc gattcctttt tcaaaaatat tcaaatgttc tggtcgtgag atactagaca
    52441 tgatcaaatg cagatcattc actttttctg catctacttt ttcaagactt tctgaaagaa
    52501 aacttgcttg tttttgattg tcgccttcta atacaacttt gtcgccggga gcaatcagtt
    52561 tttccattac ttctacgatt ttttcagttt ctacatactt gccattgagc aagttgctga
    52621 ttttttccaa tcgctgcgct tttttttcgc gtttagaagc ccagttgcgg tttggttttt
    52681 ccataattag tcatctccat ttttgatata gaaacgtggc gtcatcaata ccatgttggt
    52741 ctagattgat cgtacggatc cctttatcta caccaaattt tgtatgaatc agctttgtca
    52801 aagtattgcc ggggggaaat tcaatcgtca gatccattcc taattctttt gccaagtcca
    52861 tcatttggtt ccactgcaca gggaaagcta ggttttccaa caaatcttta cgaatctctt
    52921 ctgcgtcctg agtggctctt ccatccgtat tttttatata caaacatcta gcatcagata
    52981 gatggacaga ctgcatatac ggcttgaatt tcttgaccgt atcattcatc aagagacagt
    53041 gagatggcac agggactttc agcagcttcg ctgtttgcgc ttggtgccgt ttggcttctg
    53101 ccaacgtaac ggataaacct tcgatactcc cagataacgt atgttgcatc ggacaatttt
    53161 catttgacag ataaactgga tggtcaggat caaatgtttt tgctacaatt tcctcagctt
    53221 cgcttctcgt cacacctact actacgccca ttgcataacc gcttggataa gcttctttca
    53281 ttaagactgc gcggtgatag actagttccg ctgcattctc aaatctcaaa gatcgacaag
    53341 caacagctgc acaaaaagca ccaatcgaat gtcctgctgc aatatcaggc tgaataccgt
    53401 ttttttccat ttcatctaaa taaaatactg cttttgtcag taatgctagc tggataaaaa
    53461 ccgtatcttg atagttttct tctttatcaa ctaaccgata ccccgtcaca ttttctaaac
    53521 gttccatgta catttcaggg atatccgcta acatccctaa cttctgactg ccttgtccag
    53581 gaaataagaa acctgtactc atgagcctcg cctcctcagc acttactttt tccactgcta
    53641 tcttagtcaa tttctgctat aataaaaaat gccaaaaaaa gaaaaaacca taacaaaaag
    53701 gtatggatgg gatggataat tatgaaattg cttcatctgc agtattttaa aaaagtattc
    53761 gaaacaaaaa atgttaccca agcagccaaa gaattattta tttctcaacc tgccttgagc
    53821 cgtgcgatca aacatttgga aaacgaatta ggtgtccctt tgttttacca tagcggcaga
    53881 aacattgaga cgaccgttta tgcagaagaa ttttatccat atgcagtcaa gacattagac
    53941 acattagaag aaggaatcaa attattgtct tcggtcaatg agaagatcgt tacttccgtc
    54001 atattgtatt tagaggtagc ctctgtttct attccgaact tggtccgaat ttttatggaa
    54061 aagcatcctg atatccaatt aacgatcatg caacatcatt taccagatga tattaccgat
    54121 cctgtactgt acatcacttc ggagatgaag cctggcatga ccaatattcc tatcatgaaa
    54181 gaacctgttt atgtcgccat tcctaagaaa catcctcttg ctaaaaaaga acatctgcaa
    54241 ttagaagata tccaacgcac ccccatcttg atgctgtcaa agagcaacgc cttccgtcat
    54301 acactggatg cagcacttga agcgaaaagt atcgaattga cgatcagttc gacgacagat
    54361 gatcctgcga cattacgttc tattgtcaag caaggattag ctgttagctt tttcccaaaa
    54421 gtttcttggt cttacgataa aaatgatcct ttcgtcttgc gggaaatcaa agatttgccc
    54481 ttgacacgaa caatttattt atcttctagc ttcacagaag atcatccttt gatcaaaatg
    54541 attgcaaaaa cgatgcggga attttattta ggaaaataaa aaaaaaagag ctgggacaaa
    54601 attcttgtcg cagctctttt ttcgaacaaa cggtgttcca gaagtaattt cttagaaaat
    54661 aagccgaaat ttcccaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
    54721 agctaaacat ttctgtcaca acctcttctc tattgtccta gacattcatt gcgttgattt
    54781 aaaacttcca tcaatcaaat ctgctagttc atctagcttt tctattttac gtagatcaat
    54841 agagatccaa tgttctttat tcatgtgata ggctggaaaa tacccctctt ttcctcgcaa
    54901 aattccgcca agttcaggat cgaccttcaa atcaagaata ttgatgatcc tatggtcact
    54961 tgcatcatca tagatttttt cttctggaac agacatcagg accccaaacc attttttgct
    55021 tttttgatgt ctgaatacgc aataatcagg aaatttttta aaaatatatt ctggagacaa
    55081 attgtatgtt tcattgatgt gatcgattag ttcttttttc gttagcatct gcttccatct
    55141 ctttcttatt gattcgcttt attcaaagat a
//
LOCUS       DAZULG010000006        51464 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000006 DAZULG010000000
VERSION     DAZULG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.006"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..595
                     /locus_tag="ACG9CY_000291"
     CDS             275..595
                     /locus_tag="ACG9CY_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS4936836.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            749..1654
                     /locus_tag="ACG9CY_000292"
     CDS             749..1654
                     /locus_tag="ACG9CY_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4936837.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1651..2424
                     /locus_tag="ACG9CY_000293"
     CDS             1651..2424
                     /locus_tag="ACG9CY_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4936838.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2605..4029)
                     /locus_tag="ACG9CY_000294"
     CDS             complement(2605..4029)
                     /locus_tag="ACG9CY_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS4936839.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4240..4686
                     /gene="nrdI"
                     /locus_tag="ACG9CY_000295"
     CDS             4240..4686
                     /gene="nrdI"
                     /locus_tag="ACG9CY_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4936840.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4751..6733)
                     /locus_tag="ACG9CY_000296"
     CDS             complement(4751..6733)
                     /locus_tag="ACG9CY_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS4936841.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6747..7643)
                     /gene="murQ"
                     /locus_tag="ACG9CY_000297"
     CDS             complement(6747..7643)
                     /gene="murQ"
                     /locus_tag="ACG9CY_000297"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4936842.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7646..8707)
                     /locus_tag="ACG9CY_000298"
     CDS             complement(7646..8707)
                     /locus_tag="ACG9CY_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4936843.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8918..9445)
                     /locus_tag="ACG9CY_000299"
     CDS             complement(8918..9445)
                     /locus_tag="ACG9CY_000299"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4936844.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9512..11014)
                     /gene="eat(A)"
                     /locus_tag="ACG9CY_000300"
     CDS             complement(9512..11014)
                     /gene="eat(A)"
                     /locus_tag="ACG9CY_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4936845.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11272..12393)
                     /locus_tag="ACG9CY_000301"
     CDS             complement(11272..12393)
                     /locus_tag="ACG9CY_000301"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4936846.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12519..13460
                     /locus_tag="ACG9CY_000302"
     CDS             12519..13460
                     /locus_tag="ACG9CY_000302"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4936847.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13610..14506)
                     /locus_tag="ACG9CY_000303"
     CDS             complement(13610..14506)
                     /locus_tag="ACG9CY_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4936848.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14775..16115
                     /locus_tag="ACG9CY_000304"
     CDS             14775..16115
                     /locus_tag="ACG9CY_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4936849.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16333..16776
                     /locus_tag="ACG9CY_000305"
     CDS             16333..16776
                     /locus_tag="ACG9CY_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS4936850.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17280..18698)
                     /gene="nhaC"
                     /locus_tag="ACG9CY_000306"
     CDS             complement(17280..18698)
                     /gene="nhaC"
                     /locus_tag="ACG9CY_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4936851.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19101..20324)
                     /locus_tag="ACG9CY_000307"
     CDS             complement(19101..20324)
                     /locus_tag="ACG9CY_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4936852.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20355..21194)
                     /locus_tag="ACG9CY_000308"
     CDS             complement(20355..21194)
                     /locus_tag="ACG9CY_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4936853.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21211..21891)
                     /locus_tag="ACG9CY_000309"
     CDS             complement(21211..21891)
                     /locus_tag="ACG9CY_000309"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4936854.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21893..22966)
                     /locus_tag="ACG9CY_000310"
     CDS             complement(21893..22966)
                     /locus_tag="ACG9CY_000310"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4936855.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23022..23260)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23303..23536)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23613..24284)
                     /locus_tag="ACG9CY_000311"
     CDS             complement(23613..24284)
                     /locus_tag="ACG9CY_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4936856.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24371..27019)
                     /gene="mgtA"
                     /locus_tag="ACG9CY_000312"
     CDS             complement(24371..27019)
                     /gene="mgtA"
                     /locus_tag="ACG9CY_000312"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS4936857.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27361..27535)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27708..28145
                     /gene="ndk"
                     /locus_tag="ACG9CY_000313"
     CDS             27708..28145
                     /gene="ndk"
                     /locus_tag="ACG9CY_000313"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS4936858.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28167..29033)
                     /locus_tag="ACG9CY_000314"
     CDS             complement(28167..29033)
                     /locus_tag="ACG9CY_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4936859.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29193..31286)
                     /locus_tag="ACG9CY_000315"
     CDS             complement(29193..31286)
                     /locus_tag="ACG9CY_000315"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4936860.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32035..32658)
                     /locus_tag="ACG9CY_000316"
     CDS             complement(32035..32658)
                     /locus_tag="ACG9CY_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936861.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32817..34214)
                     /locus_tag="ACG9CY_000317"
     CDS             complement(32817..34214)
                     /locus_tag="ACG9CY_000317"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4936862.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34246..35217)
                     /locus_tag="ACG9CY_000318"
     CDS             complement(34246..35217)
                     /locus_tag="ACG9CY_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4936863.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35591..36016
                     /locus_tag="ACG9CY_000319"
     CDS             35591..36016
                     /locus_tag="ACG9CY_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936864.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36504..37205)
                     /locus_tag="ACG9CY_000320"
     CDS             complement(36504..37205)
                     /locus_tag="ACG9CY_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4936865.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37221..37661)
                     /locus_tag="ACG9CY_000321"
     CDS             complement(37221..37661)
                     /locus_tag="ACG9CY_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4936866.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37661..38236)
                     /locus_tag="ACG9CY_000322"
     CDS             complement(37661..38236)
                     /locus_tag="ACG9CY_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS4936867.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            38477..38686
                     /locus_tag="ACG9CY_000323"
     CDS             38477..38686
                     /locus_tag="ACG9CY_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFS4936868.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(39084..39596)
                     /locus_tag="ACG9CY_000324"
                     /pseudo
     CDS             complement(39084..39596)
                     /locus_tag="ACG9CY_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39722..40423)
                     /locus_tag="ACG9CY_000325"
     CDS             complement(39722..40423)
                     /locus_tag="ACG9CY_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4936869.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40531..41974)
                     /locus_tag="ACG9CY_000326"
                     /pseudo
     CDS             complement(40531..41974)
                     /locus_tag="ACG9CY_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(41988..43853)
                     /locus_tag="ACG9CY_000327"
     CDS             complement(41988..43853)
                     /locus_tag="ACG9CY_000327"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4936870.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(43974..44826)
                     /gene="licT"
                     /locus_tag="ACG9CY_000328"
                     /pseudo
     CDS             complement(43974..44826)
                     /gene="licT"
                     /locus_tag="ACG9CY_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            complement(45136..45849)
                     /locus_tag="ACG9CY_000329"
     CDS             complement(45136..45849)
                     /locus_tag="ACG9CY_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4936871.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            complement(45917..46243)
                     /locus_tag="ACG9CY_000330"
     CDS             complement(45917..46243)
                     /locus_tag="ACG9CY_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4936872.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            complement(46365..46619)
                     /locus_tag="ACG9CY_000331"
                     /pseudo
     CDS             complement(46365..46619)
                     /locus_tag="ACG9CY_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(46914..48167)
                     /locus_tag="ACG9CY_000332"
     CDS             complement(46914..48167)
                     /locus_tag="ACG9CY_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4936873.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            48228..48968
                     /locus_tag="ACG9CY_000333"
     CDS             48228..48968
                     /locus_tag="ACG9CY_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4936874.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(49054..49812)
                     /locus_tag="ACG9CY_000334"
     CDS             complement(49054..49812)
                     /locus_tag="ACG9CY_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4936875.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(49809..50837)
                     /locus_tag="ACG9CY_000335"
     CDS             complement(49809..50837)
                     /locus_tag="ACG9CY_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4936876.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            complement(50857..>51464)
                     /locus_tag="ACG9CY_000336"
     CDS             complement(50857..>51464)
                     /locus_tag="ACG9CY_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936877.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
BASE COUNT        14833 a        10764 c         8873 g        16994 t
ORIGIN      
        1 ctgttttttt taaatgatta ataaataaca acttctttct atgaaattgc aaagcattca
       61 tcttcttttc cgctaacgat atatccgtca gtgcttggta cactttacta aaaagtatga
      121 gattgccgat atctggatag gtgtgctata ctacagaaaa agatatacaa tctgccaccg
      181 ggtaggataa agaggatgat cattccatta ggtctttgaa gggatgattt tcctcttttt
      241 gtgtattttt ttatagttaa ggaggaatgt aagtatggca tggatggaat taatcatagc
      301 aggaattttg gaagtattct ggtcaacgat gatgaaatgg agtgatggtt tttctaaaat
      361 caattattct ttttacacag tgattggcat gattgctagt ttttactttt tgtcaaaggc
      421 aatcaaaagt ttgccgatga gtctctctta tcctatctgg acagggattg gcgcagttgg
      481 ttcggtcata attggtgtgg tcttgtttca tgataagctg aatctttcca cttggttctt
      541 tgtggggatg ctactcatca gtatcattgg catcaagatt actagcggcc actagacact
      601 tggaataagt actgatcaga aaaaataagc ctttttattt aatattcaat ttttttatga
      661 aaaaataaga aataagtcca atgacctagc gacttctcca ctcatcaggt gtatgcttgt
      721 actatctata gaaaaggagt gaatattttt gaaaagtctt tcggtccaag aattatccaa
      781 gaactttggt cacaaacaag ccctcagcga tgtcagtttt agcttatccc ctggcaaaat
      841 cgttggatta gtaggtccta atggcgctgg aaaaacaacg atcatgaaag ctattttagg
      901 actgatccat tactcagatg gaacaatctg tattggtgaa aatgagattt ctccctctag
      961 tcataaaggg ttggaacaag taggagcatt gatcgaatac ccgggtatct atccattctt
     1021 aacaggctat gatcatctga agttattctc tgaaaccaac gatgtatctg ctatcgacac
     1081 tatcgtgaat cagctcaaga tggaaaagta cattcacaaa aaagcgaaat cctactcgtt
     1141 gggaatgaaa caaaaattgg gaattgctct tgccttactg aaccatccgt ctatcgttat
     1201 tttagatgag ccgatgaacg gtttggatcc acaagcaaca agagatgtgc gaaaaatcat
     1261 taccgacttg gctactcagg gcacctcttt cttgatctcc agtcacttac tcagcgagct
     1321 tgaaaaaatg gtcgatgagc tgatcctgat caatcaagga aagatcatca aacaatgtac
     1381 aatggctgaa ttgaaccaaa cagcgcaaga tttcatcgtt atctctacaa caaatgatct
     1441 agctgctcga acgatcttga cagaagctgg atatactttg gaagatcctg ataaaatcaa
     1501 gcttttaaaa gaaaacgaag aattattagg aaccgtgatc cagctattgt ctgcacaaaa
     1561 tatccatgtg acagacgtcc agcatctgac gaatgatttg gaatcttctc tattggaaat
     1621 tctggattca gtaaaggggg aagaagaaaa atgacattgc ttgtaaaaca agaattattt
     1681 aaattgatca agaaaaagtc aactgctgtt ttatctgttc tattagttgt tttgctcata
     1741 ggaacagcac ttctagccaa aaaatacacg acgatcattg atccagttga aatgactgct
     1801 cagcttttta gtgccacatc ttggattgtc tttatcatga ttgcagctgc aagcacgatc
     1861 atttcgatgg aagcacaata tggtacattg aaaaatctcc tttatcgaaa gtattctcga
     1921 ggagaaatat tagtaagcaa atggatcaca ttagttattt attctgttta tttatacctt
     1981 ttagctatta ttgtcactgt gttgatgaaa ctgattcttt tcccttcgat ttccttcacg
     2041 gagaaggttt ctactggtca aacactgatc caaagcttgt tcacttatac tttgggtagt
     2101 tatatcggtt tatggctgat tttaagttta gtgttgatga ttgcttgctt tatcaatagt
     2161 tccggcgcat ccatctcagc aggaatcgtc ttttattttg cttcgtcaat catatctggt
     2221 atccttattg cactcatcca aaaatgggag tggatcaaat ggaatccgat cagtatgttg
     2281 aatctgcaaa accaaattgg aaacgaagag atcatgaagc aattgactca tctttctact
     2341 aatcaaatgc tattcggtaa tttagcttac atcgttttgt tcttagcatt aggatatctc
     2401 gtatttaagc gaaaaaatgt atgatgactc atcaaaaaag aggctgtgaa agagacgttc
     2461 agccttattg ccgattggct gtctcttaaa caccgtttat tctgaaaaag gagctgtgac
     2521 aacatttgtg tcacagctcc tttcctagta tgtttttgaa taaacgacag gctcacaacc
     2581 tctctttata cttcttttat acttctatac gtgaagcatc ccagtggcaa ataaaaccat
     2641 tgcaatcact ccgaccatcg tcacacctat aagtaaaatc ttatctaagt ggatatgttg
     2701 tacaggctcg ttttgctcct tacgtgcttt gaggtaaaaa taaatgcctg gcaagaaaag
     2761 aacaagaaca agcattactt cattaaaggc tacgactagc atagcccata cttgaaaggc
     2821 acttgctaaa agccctacta aaaattggac ccattctttc cgctgataag aaagtttcat
     2881 ttggtacaat cctaagaaaa gccacgtgaa gaaaatagct gctgtaccaa gtgtgtaagc
     2941 aaagttataa gcactgtcag tgaataataa tgaaagtaaa aagacattcg tacataacgt
     3001 cgtcaatacg atagcataag ttggtgcccc atttttgttg agttttcccc atgctttagg
     3061 caaaagtccg tcttcagcca tgtttcccat tgtttcaatc ggtaagatcg tccatgagag
     3121 ccaaacacca actaaagaaa tgatcaaacc gccgttgatc acgactgctc cccatttacc
     3181 aaccatcatt tcaaacacat agcccatagc aggctgcgcc ttgatcgttt gcagctgatt
     3241 catcgtcatt gtcccatatg gaagcatgga aaccaagacg taaataataa tcaagccgaa
     3301 tatccctaga atcgttgctt tcccaacatc tgattgtttt tttgctcgtg aacctacgac
     3361 agaggctcct tcaataccaa caaataccca aagcatcacc atgatgcaac tcaatacttg
     3421 actggaaaac gggatcggct gtccattcgc attcaatata atgtcgctag aaaagttatc
     3481 aatgaattgt tggatcgaaa acgtgctgat agctactaca ataaataaaa ataatggcac
     3541 taatttacag aatgtgacga tagcattgac gaaactagca ttttcgactc cccgattcac
     3601 tagccatgca tatccccata aaaagactgt actcatcacg atcgatgcca gattttgccc
     3661 accttcaaat actgggaaga aatatcctaa tgcgctcatc aacaatgttg caaaagctac
     3721 gttgcctaac cagcatgtga gccagtaacc ccaaccgctg ataaaaccag cgaaacggcc
     3781 aaaaccttct tctgcgtaac taaagatccc gccttttaaa tctggacgtt ttttattgat
     3841 attattgatc gacaaggaga gcatcaacat tgctgttcct acgagtaccc aactgaaaat
     3901 agctgggccg ccagccgatc cggagatgtc cgtcataata ccaaatacgc cacctccaat
     3961 cgctgaacca attactaagg cagcaagtgg aattaaacca attccttttt tctgttgctc
     4021 tgtatccatg taagcatcct ccaaaataaa accgctttta tcaaaaagta taacaaacct
     4081 tttttatttt tacttaatta ataaaaactt taatagaaat ataattctac cggctttttt
     4141 ctcattatac aagcaaagcg cctagtccct ttccaaatta caccaccaaa aaacttaaaa
     4201 aagatgctat actaaaagga aagaattgga ggatgatcca tgaacatttt atatatatcg
     4261 atttcaggaa atacgcgtgc ctttgccaaa catctagcag aatatgctga aaaaatgcat
     4321 gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa tccatgaaaa tagtgatttt
     4381 acgaaagaaa cagaaccatt ttttactttc gttcctactt atttagacgg cggaaatggt
     4441 ttagataacg gtgatacaga aatcttgacc gaaacgatgc gtgaatatct ggaacatgaa
     4501 gacaatcata aactttgtct tggtgtagtc ggtagcggaa ataaaaactt caataatcag
     4561 tattgtctaa cagccaaaca atatgcacaa acatttggct ttcctttctt ggcagattat
     4621 gaattacggg gaacaccatc tgatgtgaca cgaatttatc agattttagc agaaaacaat
     4681 aaataataaa aaagaagaag ctgaaaaata gatttcagtt tcttctttcg tttctattgt
     4741 ttctatcata ttaagctttg acatccatca caggtgcttt tgcttttgct ttacctgttt
     4801 tttccaagac aaagtttttt acacgatcca tattggttac caagacgatc atggatgttt
     4861 ctttgcctgc tgctttgatc gcttctaagt cagcttctgc tacaagcgta tcaggtgtca
     4921 cttgctgccc ttctgttact ttcacatcaa atggcgtacc gcctaagtca actgtgttga
     4981 ttcccatatg aagcaagatt tctaaaccat ttggcgttgt gatcccaata gcgtgtttcg
     5041 ttgggaagac agagctgact accccagtaa ctggagaata gatatgtccg ttttctggtt
     5101 ctacaaagta gccttcaccc atcatctttt gtgcaaaaac tggatcactt gctttatcta
     5161 aagaaacaac tgttccgtca gcaactgatg agaggatagt cacactttca gctgaagttt
     5221 gaggtactac tttttcaggc acttcattta ctgtggctgt ttcagtcgct aattcttctt
     5281 tttctaaacg gtctttagga ataccgaaga agaatgtagc aacgaagcca ccagcataag
     5341 ctgccaataa tccaaggatg tagcctaacc atttaccgtc agagatcaat gggatcaaag
     5401 cggctcctga aggtccaata gcgatagccc cgacatttcc gatcattccg atcactgccc
     5461 cgccgatacc tccaccgata caagcagtaa taaatgggcg tcctagaggt aatgttactc
     5521 cgtaaatcaa tggttcgccg attcctagga tcccgactgg taaagctcct ttgatcattt
     5581 ctgttaattc tttatctttt ttgcagcgaa tccataatgc caatgctgca cccacttgtc
     5641 ctgcaccagc catcgcaaga attggaagca atagcgtacg accagtttca tcaatcattt
     5701 gcacatggat tggagtcaaa atttgatgca gtccgaacat aaccatcggc aagaataatg
     5761 tgcctagaac gaagccggca aatgctccac caacgccaag tacccagttg atcccaccga
     5821 ccatgccatc agaaatgaaa cctgctaaag gcatgatgaa gaaaatttca atcagaccga
     5881 tggcaatcaa tgaaagagtt ggtgtaacga tgatatctac cgcatcaggt actactttat
     5941 gcagtttttt ctcaacgatt gacaatagcc aaacagcgaa gatgacacct aggatcccac
     6001 cttgtccagc tgcaagagct tcaccagtaa ataagttttt cagtggtgct tccggattca
     6061 tccctgtcag catcacaacg gccccgataa ctccaccaag agttggtgtt gcaccaaaaa
     6121 cttgtgcagc attgatacct gtatagataa ctaggtagct aaacatcccg tttttcaaga
     6181 tgttcatcac atcgatatat tgttgccatg ttgctgcatc aagtgttccg gctgtgacta
     6241 agtttgctaa aactgcggca atccctgcca cgatcccagt accgacaaat gcaggaatca
     6301 ttggcacaaa gatattagaa atgtctttta ataccgattt gattttagat ggtttttgtt
     6361 tcgatttttg tgccgctttc atttcttgtg cccgttcgtt tacacggtct tttccagatg
     6421 ctgatgaatt tgctgtagct gtacttacag aagaaagttt ttctcctaat ttcacgcctg
     6481 ccaaatcaac catttgctgt gctactttgt tcactttccc aggtccgatg atgacttgaa
     6541 gctgttcatc ttctactaca ccaagcactc ctggaatcgc ttttaattca gagacattta
     6601 caagatcatc attgatgatc ttcatacgca cacgagtcat gcagtggaca atgctttcta
     6661 cattgcccat gccgcctgcc cctttataaa tttgttttgc cagttcttcc aaagtcagtt
     6721 cgttttttgc catggtcttt tccccctcat aaggtccctt ttacaaatcc gtttgcttca
     6781 gctaattttg cttgagcttc ctctttatca ctatttgtca aaatcatgac gatagccagc
     6841 ttaacatttt gatctgcttc ttcaaagtac ttttcagctg tttcgtaatc agcttctgtc
     6901 gcttgcatga tgattcgttt tgagcgttcg attagttttt catttgttgg tttgacatcg
     6961 accatcaagt tattgaatac tttacctata ccaatcatac tgatagtaga aatcatattc
     7021 aagataagtt tttgtgctgt tcctgacttc aatcgagtag agcctgtcaa aaattctgga
     7081 cctgcatcga cttcgattgg gaaatccgcg tgtttactga tttcggcttg tttattgcaa
     7141 gaaatcgttc ctgtgacagc accgatcgat tttgcgtaat ctaatccacc gatcacatat
     7201 ggtgtacgac cgctagctgc gataccaata actatatcat tttttgtcaa attcagcttg
     7261 attagatctt cttgccctag ctcataagaa tcttcggccc cttcgacagc tacagtcatt
     7321 gcttcttctc caccagcaat tagacctatg accatttccg gttctacacc gaatgtcggt
     7381 acgcattcag cagcatctaa cacacctagt cttccacttg ttccggctcc catataaatc
     7441 agacgtccac cttttttaaa ggactcaatc gtgttagcaa tcactggttc gatacttgtc
     7501 agttcttttt caaccgcagc ggccactttc tgatcttctt tgttcattag ttgaacggct
     7561 tgagaaatac tcatctcatc taatccaaaa gtttcttggt ttctgcgttc cgttgttagg
     7621 ttctctaaat tgattttgct catttttatc ttccttcttt tcttataaat tgatagttca
     7681 taccagcttt gatttggttg atcaatggta gctcttcttt gataacttta gctactacat
     7741 tcactttttc atccgctggc aaatcatatt tggttaattg gatctcaccc atatagcgca
     7801 aatacttttc gttatctaaa gtaatagacc ctttcatacg cgtagctgta tttcttgctg
     7861 ggataggcgg aatctctcga aaacgtgcat ctacgcttcg tatgacatct cttgcatcgt
     7921 cttgccgatt cgtatgcttc cctaatatat attcgtaaaa ttcttcatct acaggttccg
     7981 tatggagcaa gactgtctgt tctttttgga aagaagcaaa ttgctcctga acgttttctg
     8041 acagacctgc atctccgatg taaacaatat ccgtgttact tgctaataga tctagagcgc
     8101 ctgcaagagg atggatccct cgatgttctt ccaaagttgg caatccttga tatagtgggc
     8161 ctctcaactc tgtatctcct ggtacaaaac cttggacaag gagaccttga attttgagcc
     8221 attgattttt tttctgatac cattctttgt ccagccctgt ttcgggacgt ggataataat
     8281 tatgccaagc ttcaatctgt gagaaatcag cttctgcttc tttcaattca tcgatatctt
     8341 taggcgtgat cgtgctagca ttcaaactga ttttcaattg atgtgaccag tcggcaatct
     8401 gctgattgga aatatgataa tccattctta aacctgttac accaattgct ttcaaaggcg
     8461 ttagatcttc taatgaaaaa cccgctcgtt ccaatgcctc tcctgaaata tcgaccatca
     8521 attctagttt attctttttc gcttcgcttc ctagttcgat caatcgcttt tgatatgccg
     8581 aaacatcatc ttctggtata tgcatggaag tgaaaatacc gacaaagcca ttggatgcca
     8641 tttggcggat atactgtttc ttctcttctg acaaagaatc gttcataaat attgaaaagc
     8701 caaacatatt atccctcttt ttctgtatcc gtcttcattt ctatgactag agtatatatc
     8761 taaagaaaca aaatttcaat acttttatta aaataattaa atttcatttc taaaggttga
     8821 gtttatatta tcaaaagaat tgtcataacc taaaaaaggc actgctaagc aaaagcagtg
     8881 ccttgaaata ttttatttaa ataacacaaa aacataatta ctcataagaa accaattctg
     8941 tattatctac catgaatgta aagaacccct cattaggcat cggatgaaaa taagcattga
     9001 tcactcttat caaggcatta tggcaaacta acagatagac attttcttcg tcttcgatgc
     9061 attctttcaa taaaggaacg atccgtgcat acacatctaa gactgactcc ccatttggga
     9121 atcgtacagc gaacgctaaa cgtgcttttt gaaagttctc atctttgctt ggcagtccat
     9181 cataatcccc aaaatccatt tctaccaatc gttcatcaac ggtcagcggc aagctgagac
     9241 gatcagcaac tgcttgtgct gtatctcttg ctcgttgaag aggggaatgg atgattttcg
     9301 tgattggaac aggcaaatct actacctttt ctgctagttt ctgtgcctgc atctgtcctt
     9361 tttcagtcag cggcacatct gcccgtccgc aaactaatcc ttgtgcgttc caagttgttt
     9421 ctccgtgtct cgttacataa agcatgtgat tctctccttc gttggcaaag ttccccttaa
     9481 ccataccttg ttgttcttga gattacaagg gttagattct ctcaagagaa ataatctcag
     9541 tagatacttt atccaggaag gtttgatcat gttccactag taacatggca ggtttcacgt
     9601 tcaagatcag ttgttctaat tgttcttgat tgaagacatc caaataattc aatggttcat
     9661 cccatgtata tagttcagct ggctgtgata aagatttagc caattccact tttttccgtt
     9721 ggcccatact catctgctcg atcttgttat gaaaaacatc tctttccatc ccaagctttc
     9781 ggaggttgtt caaaaatgct tggtagtcta cttggttttt ctctgcaaat tccgccaacg
     9841 ttcctcgatt gtcttcataa ttttggcttg cataactaat gctcagatgt tttggctggt
     9901 atttttctcc tataaccttg ccgttgaatg cccccagaag ataatggata atggatgact
     9961 ttcctgcacc gttaggacct gaaatcgcca cccgctgatg aggctcgatt gtaaaatgaa
    10021 ttggctcgaa taacagattt tcatacccta attgaagatc ttctacgctt aaaagtcgct
    10081 tatggtgaga cgcttggcta ttcatcgtca aagaatcgat atattcgata tcttttaata
    10141 gtttttcttt ctcgctgatc tgggtctcca tccgattcac gattgctttg gatcgtttca
    10201 tcgtccgtgc agcatcagca cccactgctc ctttattcac tcgtctagat tcagtatcga
    10261 tgaatccgac ttgtttcttt gttttatctc cttctcggga acgagaccat tcggcttttt
    10321 cagctgctgt tttctttagc ctactgactt cttttttcaa tttttcattt tgagccattt
    10381 caaattcatc acgaagtttt ttctgttctt catagataga gaaattccct tgataaagtt
    10441 ccagttgact tttttcgatt gctaaaacat ggtccactac ttcatcgata aatccccggt
    10501 catggctgac cacgatgaag ccttgttttt tctttttcaa ataagccgct acttgttttc
    10561 taccagagat atccaaatga ttcgttggtt catcgattaa cgggaaatga gtgtcatcca
    10621 caaataaaag tgccagtagg acttttgtct tctctccccc cgatagtgtg ctgaattctc
    10681 tccataagat ttcaggatct gtttgcatca attggagctc tctttcgatt tcccatatct
    10741 caaaatccgt aatatcattt aacacgtaat aggttaaacg ttctttatct tttgtctgtt
    10801 gcgggaaata ggcaaattct tgctgatgga ttacttgccc ttgataaggt agtttgtttt
    10861 gtagaatatt cagtaaagtt gtctttcctc gaccgtttcg tccgataagt cctagtttcc
    10921 attgtgtgtc aaaatttaga tttgcttgtt caaataataa tgtgccttgg ctgtcgtagc
    10981 cgaatgtcag attttttatt tcgattttag acattttcca tcacctcgta aaaaaagacc
    11041 agtcctattt tcagaactgg ctaaagagga tgcaagggtt ggatacacac aaaaagaccc
    11101 atacatcgaa aaaaccaagc caatcccgaa aatctgcaca ctgcttcctt gtctccacaa
    11161 ggtacagtct gaatgcagct ttcatcaatc gacttaattt ttcaatgcac ggatccctca
    11221 ctttgtccta tatttgaaat tacgatagca taaagaaaat gattttgtca actacttcgg
    11281 gaggatggtg aaaaatctgc taagtgtctg tcctaagatt tttgtttttt ctaatgtttg
    11341 cctgtgttcc attgcttctt ctaaacctat tggacccaaa tgaatactta agacaattgg
    11401 gagaaaatca gaaagtcctt ctagctcttg gtctctactt ccgcaaatag cgattattgg
    11461 ttttcgatat ttttttgcaa gcactgctac accgtatgga acttttccgt ggatcgtttg
    11521 ctgatcgatc cgcccttctc ctgtaatgat gacatctgct tgttcgatct ttttttccaa
    11581 tccagtgatt tggctgacta aatcgaaacc agaaaccatc ttcgctccca acaaaaaaag
    11641 ggctccacca ataccgcctg ctgctccact tcctggaata tcatctaaat ccacctccca
    11701 gaaatctttt atttgctgag caatctttcc tgcttgttta tctaagaaag ctagcgtcgt
    11761 atctattcct cctttttgtc ttccaaattg ccaagcagct ccttgttgac ctgtatatgg
    11821 attggtaaca tctgcagcga taatcaattt tactttagaa acaagaacgt ttcttcgatc
    11881 aaaatgaagt tttttagtag ataataaagg atttccttct gtgtaccctt ctaaagaagc
    11941 acccaaggat tggagtaatc ctagaccacc atcgctacac ccgcttcctc ctaaagtgac
    12001 aataatttgt tctacttctt gaagcagtgc atctttaata agttctccta aggcataact
    12061 ggaagcaagt cggatagttt gatccgttgg tttatcaagt agatctaacc ccaatgcttg
    12121 tgcagattcg atgactgcgg tgttttttcc ttgccaagtt gtcaataaat agctgatttt
    12181 tcgctgacgg aataagagat ccatcccatc aatggtacgc catgttcccc tgagcgcgta
    12241 atagattgct tctgcagtat tttctccgcc atctgcaatc ggcacattga tttttcttcc
    12301 tttacgatct tcccagccat ttagaactgc ctgatttagt tcaaatgaag aagcacttcc
    12361 tttaaatgaa tcaattgccg ccaaaatatc catctgtatc cctcactttc ttcttaagta
    12421 agacactttt tttcattttt ttcaaacaaa acaatgctat tttcttccat ttttctatat
    12481 actaaaaggg aactcattga ataaaggaga gaaaaaacat gaaaatcgca atagctgggg
    12541 ccggagcaat gggcagccga atcggattga tgctacatca aagcggaaat gaggtcctct
    12601 tgatcgatcg ttggcctgct cacattgaag ccattcgtac aaatgggttg attgctgact
    12661 ttaacggtaa agaagtggtc gcaaaactgc caatctattc accagaagaa atcatcgaaa
    12721 gcaacgaaca cgtggacttg atcgtagcat taacaaaagc caatcaatta gatgatatgt
    12781 tttgttcgat ccaatccatt attactgaca atacttatgt tttgtgttta ctaaatggat
    12841 taggtcatga agatgtactg gaaaaatatg tgccaaagaa aaatatctta tttgggatca
    12901 cgatgtggac agctggtcta gctggtcccg gaaaagtcac attgttaggt gatggagaaa
    12961 tcgaactaga gaacttggaa ccagaaggcg aagcatttac aaaaaaagtt gtagaagtct
    13021 ttcaagaagc aaatttaaat ccgatctata gccataacgt tcgttattcc atttggcgaa
    13081 aagcttgtgt aaatggaaca ttgaatggtt tatgcacgat tttggattgt aatattgctg
    13141 aactaggcgc gcaaaaagcg gcagagagca tggttcgaac aatcgtatct gaatttgctt
    13201 ctatcgcagc aaaagaagga atcatactag atcaagaaga agtgtaccaa catattgctt
    13261 ctacttatga tcctgacaac atcgggttgc attacccatc tatgtaccaa gacctgatca
    13321 aaaaccatcg tttgactgaa atcgactaca tcaatggtgc catttggcga aaaggacaaa
    13381 aatatgatat tgctacacca tattgtgcct ttttgaccca attagtccat gcaaaagaag
    13441 gaatccttgg cgcagaataa aaggctctct gatgagaatc agctgaatca gcagctggtt
    13501 ctcttttttt aagcccaata aaagatcaaa aagaaaacac aactctcatg atgccataag
    13561 aattgtgctt gtttgtttca gtacataatc tcttcaaaat gtaggttttt tatttgtttt
    13621 caacagagga gaggtcatat tcgtattgct cataaatagt ggaatcgtta gtgataaatc
    13681 gttttgccca attttctgca tcataaaatt ccaatagctc tgataggatt ggcttaaatg
    13741 gtgtatgcag aaattccggt aattcatcta aatcacgttc tcgaagcaaa aaaatatttt
    13801 caggacgata aaaatgataa tgagcaatcg cttgattatc cgtcactagt ttcttttgaa
    13861 aatacatgga ttcaaatgtc cgcaaagtca ctccttgctg accctcccgc aacagttcca
    13921 gaatcccgcg cgattcccct gtttttttca ggtactcttc ataagtaaga tatccaggtt
    13981 gttcattccc tcgatgtgga agaatgaaat agttggtcgt aacatctctt tttataaatt
    14041 cctgcttata gctttctgct cgttcttttc tcccattatt cgttgcacca aaaaatagat
    14101 ccgtctttgg ttcgctagaa gaaaaattca tatgtttcga ataaaatgaa ctattatgtt
    14161 tcagcccaag ttcttttgct tccaaaaaat caaaatgata aatctcatcg attgccggtt
    14221 catcttgtgc ctgcttcagc atcgcctcgt gttctgcttt gaagtgattc caaaaaaata
    14281 acacaatttt tgcattggta aatgggcgca cgcgtcgagc catctccata tcaaatccat
    14341 aatctaaaaa aacgatcagc tgataatttg ccaactgttg tctagctgat ttaatgaatt
    14401 ccgactgtag taaatctttt ccggaccaaa catctaaaga gcggatggaa ggcaaaaaat
    14461 aaagtgattt attttttgca actagcagta cgtcattttt gttcatcttt tccgcctcac
    14521 tttctctgtc actttttcct atgatagcct gtttaaatag taaaaagcca gtaaaacgtt
    14581 tcctatattt gtatgaaaat ttaacatttg tatgaaaata tgatgaaata aatcattctt
    14641 ctattaaaat tcgcctaaaa gttttctgaa tagttgtaat tgtctgaaaa ttttttatca
    14701 ttagtcattg ttaaatattg ctatttattc ttaatataaa ttttgattgt gaatttaaaa
    14761 gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca acttagctcc agacatatca
    14821 ccatgttagc tttaggtgga gcaatcggtg caggtctatt taaaggaagt ggcgaagcga
    14881 ttgggatcgc cggcccttct gtattgattg cttttttgat tggtgggctg attttatata
    14941 tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg cgacacgcat cacggattat
    15001 ctggtttgat acgcccttat ctaggtgctc attctgctga ttttactgat tgggtctact
    15061 ggtctatgtg gattatcaat atcatcgctg aagctgttgc agctgcttcc tttttgcagt
    15121 tgtggttccc gcacattcca gcctgggtat ttgtttttat gttggctgtt ttaactacga
    15181 tcatcaatct ttattcggta cggttattcg cagaaacaga atattggctg gcttttgcta
    15241 aaatcagtgt cattatctta ctgatcattt ttgctactta tcttgtcggt cagcaaatgt
    15301 tgggcacagg cgttttccct acccttcagc aattgactga ccatggtggg tttactcctc
    15361 atggtatgaa gggaatcatc agttctttgc ttgtagttat ctattcttac ggcggttctg
    15421 aactgattgc tatcacggta agcgaagcag atgatcctaa acgtgcgatt cctaaagcaa
    15481 tcaaaggtgt catgggaaga atcatttcct tttacatcat tcctttgttt ttgcttttga
    15541 ttctttttcc ttggaatact ttagcaggca cgaatgtcag tccttttgtg atggtatttg
    15601 aaaaaatgaa tatcccattt gcagcggata tcgtcaattt tgtgattgtc cttgctttgt
    15661 tctcatctat caattcgggt gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg
    15721 atcgaaaagg ttcctctaga aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg
    15781 ctgttttatt ttgtaccagt gttctttatc ttggtgtagc tctttcttat tttgtcggtg
    15841 acaaattatt tggctatctg gcaggttccc tttcgtatac agtattattg atctggatca
    15901 ttatcagtgc ggcatccttt gtactggcac taagaaaagg aacattatgg gataaaggaa
    15961 tcagcttttt tgcattagct gtcttaggat tgattttcct tggtatcctg ttcacgaact
    16021 ccattggggt aactgtattg actggcctgc tttacctgtt catttatttc agttatcaaa
    16081 aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg atagaaaatt ttattttatt
    16141 tatgtggaat agatggagct attgaagaaa ggagttgtga caaaagtctt gttgcaactc
    16201 ctttttccga ataaacagtc tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg
    16261 caaaataatt gattgttctc catgctcatg acatgataga agaaaggaat gaaaaaaagg
    16321 agtttgtttt aaatgaaaaa gtattggaag tttttgttga tgatcggtgt ttcaacggta
    16381 gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt
    16441 acacgagtat tcatggcgtt gatgatgggt gcagtcatgg cagtcatcat gctgttgttc
    16501 atgtggaaaa tgtatgaaaa caaaaagata aatgttatga tcttagccgc tagtcttctt
    16561 gtttttgccg gttccttgtt tatggtacga agccaaacta ctgtcaacga cacagcttgg
    16621 atgaaggcca tgattcctca tcattctatc gctatcctgg caagtaaacg agctgattta
    16681 aaagatcctg aagtaaaaaa attagcggaa gaaatcattc aggcacaaga agaagagatc
    16741 gacaaaatga aaaaaatgat caaagaaatg gaatgaccat tatccgtcca aataaaaaaa
    16801 gaggatgttc cagaagttat cagcttcaag aaataagaag aagctacttc tggaacgccg
    16861 tgaataaggg gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa
    16921 tagcttctcc tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac
    16981 cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag tattagagag acaaatggaa
    17041 aaatagttcc tcatattttg ccattttttg agctaatacc gagggtcagc tgattgaaca
    17101 ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc gtattaagaa aaattttgtg
    17161 aaatggcttt ccacattttt gtataaattt ggcttaatac cgagaaactg gcttttgaat
    17221 accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc acaatccctt tttatgtttt
    17281 tattgttcta attgcgctgt cttgcttact gcttcttgcg gtttcacaaa tttttttgag
    17341 attccccaac ctgtgaaggc actgaccatc gaaaacacag gagaaagcaa actgaaaaat
    17401 acgaatggca agtaagtgag tgtaggaata ccaaatgtac ttgctacgaa acttccggca
    17461 attccccaag gcactaagta gttgattact gttccaccat cttctagcgt tctacctaat
    17521 acagttggat caaatcctgc tttttcataa gtttctttaa atgcattacc aggcagaata
    17581 acagatagga attgttcacc aataaaaata tttactccga tacttgtcat caatgttgta
    17641 aaaatcaaat ttggtgtgtt cgtcatcttt cttgatactc gttccatcac ggttgagatc
    17701 aagccaaatt cgatcaacaa tccacctaat gacaaagtta gaataatcaa tgacacagta
    17761 ggcatcatac tttcgattcc tccgcggcta agcagtgaat cgatttgttg gtttccagta
    17821 tttgaaacaa accctgattc gatgacccca gccaacttcg ttaaggaagt ttgtgggttt
    17881 tgaataaaaa tcattcctac tgctacagcg atattcaaca aaatcgttaa aattgccggc
    17941 acctttttcc atgcacacag taacattaaa aataaaggaa tcagtgacca gattgaaata
    18001 gtaaagtttg cttctagaac atcagtcact tcacgaatag atgaaagact tgcggcttta
    18061 cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg gacagttgac
    18121 cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc tgctagattt
    18181 gttgattcgg ataatggaga catcttatct ccaaaaacgg cacctgaaac aatagctcca
    18241 acaaccagcg ctggatttaa gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc
    18301 gttgacataa cagtaaaggc actccctaca gcgctaccta cgattgcaca cacaacaaat
    18361 acagaaggga caaaccactg gacactgatc aatttgaacc caatgaccat tagagttgga
    18421 atgatccctg cttgtatcca tacagcaatc aatgaaccga ctaaaataaa aataaagatt
    18481 gggatgattc ctggtttgat gccattgatg attccttcgt gcagccaatc tgtcgggtat
    18541 ctttttatca ctgcatacaa catgaccatt ccgatcgctg ctaagatcgt gatattaggt
    18601 gagaccttta atccgacaac gccaatcgca atgcctaaaa caataaccat taatacaagt
    18661 gttgcttcta aaaaacttgg tgttcctttt tttcccatga taatctctcc ttttttagtt
    18721 gttcttatgt tctaaaaaag ttaagatatc ggattactcc gccgcaattc atccgcgatt
    18781 cctcctttat aaaaataaaa agacctgttt taaactaagg acgaatgact ccgcggtacc
    18841 accttaatta aaaacagatc tgtttttcac tttcaaactt ttattcaaat gtatggactt
    18901 tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg accaccgact ttctgaacaa
    18961 aacacctaac cgctactgcg tgaaaagttg acttgtacga agtttacctt ccataagatc
    19021 atttgtcaat gaaaaagaaa atattttttc atttttctga aaattatttt tataactttc
    19081 acacacaaga ttatttttta ctagaaagta gcgccctctt gtttttttct tgctactatt
    19141 tcttgatgtt cttgcttgat tctttctaat agctttggct ctttcaacag atcaagagct
    19201 gtattggcga gtagttctgc tgcaatcgca atagaagcaa ggcctttgtc acttttagca
    19261 gcagctttga attcttctgt gtgtccggca atatatgtct cagagattgc taccgtaggc
    19321 tggattgttg gtatgacttg gctgacattt cctacatcag aagaaccgag acttgttctt
    19381 tgttccgttt ctatttcttc atctggaaca cctgcttctt ttgcatgctg gaagaataag
    19441 tcatcaaaag aaggagtaac gatgacatca tcaactgcat tttggaacaa tccaaactca
    19501 aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat tttccacttt ttcatatact
    19561 ttatctaatg tttgccgatt tcctgcacgt aaatagaaac gtccagcagc gtaatctgga
    19621 atgacgttgg cagctactcc tccgtctgta atgatcccat ggatattgac atcttttggc
    19681 aattgcaagc gtagagcgtt gattccatta aacacttgga tcaatgcttc caaagcattt
    19741 actccttttt caggtgctgc tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact
    19801 ggatcattcg ctaaagattc tgtcgttttc ccataacgaa aagctggatg gacgcataac
    19861 gctacatcga catcctcaaa aaaaccttct cgaacaaagc tgcctttagc tgatccgttc
    19921 tcgccccctt cttcccctgg ggtgccatat acacggattt ctccaccagt ttcatcaatg
    19981 aattgcttca ctatgctggc ggctaatacg gagtaagtac caaacaaatt atgcccgcat
    20041 gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa aggcaattgt gggaccgggt
    20101 ttattggaaa tataacgtgc atcaaaggct gttcggtgtc cagctacttc tttttctacc
    20161 caaaaccttt ctttttctaa ttgtttaatc aacgctgctg aggaataaaa ctcatagtta
    20221 cttacttcag gatgttcgtg gatatctaga gcaagtgctt gatattctgg taaacgatat
    20281 tggattagat ccttcaattg gtttgttgag attgttgttg tcataaaaaa ttcctcctat
    20341 gctagtcatt tttcttattt tctcccgaat gtttcccatg caggcacgct tgatcctttt
    20401 gatgttttct cgattacttt gactgtgtct tctgtttgat aagcgtctac tattttttga
    20461 tacgttttat tgttttcatc ttcttttctt gcaacgatga tattcacata aggacgtgca
    20521 tggtcatcca ctggttctaa gaagatggca tcagaagcag gattaaatcc agcatctaca
    20581 gctacaccgc tattgatcac tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa
    20641 gcatctaatt cagaaatgtc tagatttttt ctattctcgg taatatcaga aacagtgggc
    20701 gcttgtcctt ttgccttatc cactttgatc aatccagcag tttgcaataa cagaagagct
    20761 ctgccgccat ttgtaacatc attagggatt gctacttttg ctccgtcttt gatatcagct
    20821 acatccttca aagtgttaga ataaattccc agcggtgcat tgacagtatt tccgatcgat
    20881 actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa
    20941 ttcaaatcaa tcgatccatc tgctaaagcc acatttggtt gattgtaatc tgtaaatttc
    21001 acgtattcca aattaatgtt ctcctttttc aaacggtctt tcacgttgtc ccatacatct
    21061 gtatcttctc caataatccc tagttttacg atagtttctt ttttgtcagt tgtatctgct
    21121 tttccttggc tacaccctgc caaccaaata attgctacaa atgtaatgac tacaaaacga
    21181 ataatttttt tcattttttc tcctccactt tcaatgtgta atttttttaa tcagttgatt
    21241 actgataaat tgacttaaaa aaactaagat tagaatcaat aatgtagcca caatcgttac
    21301 atctgtttgg aatcgattat accctctcga tatagcaaga ttccctaatc cgcctgcacc
    21361 tactgctcca gccatggtag tcagtccgat cacgttgatg atagtcaacg ccgagacccg
    21421 aatgatacca ggcaaacctt ctaacaaata cacacgtaaa atgataccga ttggactggt
    21481 tcccatcgct tctgccgctt cgatcacccc aggatcaact tctagcagtg cgttctcgat
    21541 ttgacgagca aaaaacggaa caactccaaa aatcaatggc actaaagctg ctttcgcacc
    21601 aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa agagaaagca agataatgaa
    21661 agggatggaa cgaatgatat tgatcagctt atcaagtatt tcaaatagca gacggttagc
    21721 tagtaatcca ttagggcgtg tcactactaa cgtcacacct aagatgattc caatcatccc
    21781 gccaatgaat gctgtccaaa aagacatata tagtgtttgc ttcgtcgctt cgacaaattc
    21841 atccggaatc aaataaacat tgggcaaata tgttttaaag aaatcagtca ttttatcttc
    21901 ctccttcgat cactttgata cgactatcta aatcgttttt ttgtaaaata tttattttta
    21961 caccagattt gatcaaataa tccaatgctt ttttccgctg tttggtttct ccagctaaag
    22021 taacgatcaa actccctaac ggcgtttctt gtaataactc tacattgcca tataaaatat
    22081 tcgctgttac ttgatatttg ctgtacagat gagcgattag aggttcgttc gtctgatttc
    22141 caatataact taattccacc agccatttat tggcgatctg atcggcaaaa gcagaatggg
    22201 caatgatcgt ttctaatgct tggtcaagat gagtagctgt tcgaataaaa tctttggtca
    22261 attcttcttc aggattgctg aagatctgga tgctgctacc tttttcaacg attcttccat
    22321 cttccataac cgctaccttg ttgcagattt ctttgacaac ttgcatctca tgagtgatca
    22381 ggacgattgt taaacctaac tggcgattca atttttttaa caatgcaagg atctgaaggg
    22441 tcgttttcgg atcaagtgca ctggttgctt catcacataa taatacctta ggttcattcg
    22501 ccaaagctcg tgcgattgct actcgttgtt tttgtccccc cgacagttgt ttaggataag
    22561 cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc attcactttt tgacgacgtt
    22621 cctgtttcga ttttccagaa tactttaaag aaaaatcaac gttgtcaaaa atagttcggc
    22681 tttccattag attgaaatgc tgaaagatca taccgatggc ctttctttct tttcgcaatt
    22741 ctttgggaga gagctgactc agattcgtct gattgacaat gacttctcct gctgttggtt
    22801 tctgcagcaa attcatcaca cgaactaacg tacttttccc tgcacctgag taaccgacga
    22861 ttccataaac atcgcctttt tcaatcgtta aatctacaga atctactgcc tgtatctctt
    22921 tttctttttg tttgaaacat acagaaacat ctcgcaattc aatcattaaa attcctcctt
    22981 atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt tcttgaggac gcttttttgc
    23041 gtgttaccac ctcatgttca agctgtgttc acacacaact cctcttcaag tacgatcata
    23101 ctctagcaag atatcggtcg cagccgtcat cgccttacat ctatgctcgg gatgaattgc
    23161 tcgatggcca ttttccaata gtcgttcctt gcttcttctc agctaccgaa gctctctgtc
    23221 aaaggttctt ctatcgtact taccatttca atgcaaataa tattcaaaaa aaaagtcctt
    23281 ttactaacat aaactatgtc agtaaaagga cgacgtatcg cggtaccacc ttttttcgga
    23341 agtttccttc cctcaagccc aacatctcaa aaagagagat tggcgttcgt tttaacggtc
    23401 gaacagttcc gaatttgcct acttatcgac aaattattct ccaaggccat tttcagcttt
    23461 tcttacttga ctcttctcac cagccagagc ctctctgcaa agtttccaaa cttactttcc
    23521 ttttcaacga aatcgatatt ttgttgttgg gattaatcta catgaggaaa actgaaaagt
    23581 caagtacgaa attcaaatta ttttttctat atttattttt ctgttttttt atttaacatt
    23641 tcaataacag ttcgaagatc ttttccttcc gcaagaagtt tcaatttttc cttttcttcg
    23701 acggtataat ttcggacaaa actggaatca ggtatcaaaa caacgcctaa tcctgctcgt
    23761 gaagcagctg ttgctcctac taaagaatct tccactgcaa aggctgaact agcttctaat
    23821 cctgttcgtt ccaaagctgt cagataagga atcggtgaag gcttgttctc cgacacttcg
    23881 tctccgaatg taaacgtgtc caagtaatca gctaatttaa agtgatccaa gatatctgtt
    23941 gctcttttcg taacagtggt cgtagcaatg cctagtttca tatgacgttc ttttaagtct
    24001 gccaatattt cttttgcata tggttttaag tggatggctc cgcttgtcag ctgttgataa
    24061 aaatattctt cacgcttcgc tcggacttcc tgtacttttt cagggctacc tgctgctttc
    24121 actaaaatat caaaactttg tttccagttt tttccgcccc aagcgtcaac gatttctttt
    24181 gggatatgga tatcaaattg ctgaaatccc caaagccagc catcacgata cgttttttct
    24241 gtgtcaacta acaaaccatc taaatcaaat attgcacctt tcatctatac attaatcctt
    24301 tccaaaacaa tacacatagt ttagcacgac tggtccaaaa agaacagaag caaagaacca
    24361 gccttctcgt ttataagagt tcttggaatc gacggatata gattttcttc acagctgtca
    24421 caagcgccag ataagcgatg actgtagcaa ataaccataa ccagtaattg ccaggaagtg
    24481 gcatcaagcc taattcagca ccaaaacttg taaaaggaag gacagaaccg acaataattc
    24541 ctaatgtagt gataacaaac atggcaaatg ttgcgttgct ttggataaat ggtactttcg
    24601 gtgtacgcag tgcatggagc actaaggttt gtgtccatag tgattcgaca aaccatcctg
    24661 catggaataa tgcgatgaaa atcactcgct gtgaactatc caatgtatgg aaacttccgc
    24721 ccactacagc aggacaaata acaaaataca tcaacaagta agtagtaata tcaaatatcg
    24781 aacttgttgg accaaagtaa accatgaatt ccccaatact agatgcattc catttttttg
    24841 gctcgtccaa atattcttta tccatccgat cccatggcaa ggaaatgcag gaaacatcat
    24901 agatcagatt caaaaacaaa agttgtaaag gcagcattgg taaaaatggc aggaacgtac
    24961 ttgcaatcag cacggaaaac atattgccaa aattagaact tgctgtagcc ttgatatatt
    25021 tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat
    25081 ctttttctaa aaggatcacg tctgcagatt ctttcgcaat atccacagct gtatctacag
    25141 aaataccgat atcagccact ttcattgcag gagcgtcatt gataccatct cctaaaaatc
    25201 cgacagtatg accattttta cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca
    25261 attttacgaa tacttcatgg tcttcagcta cttgtgctaa ttcatgatca ctcatttccg
    25321 agattttctc accggtaacc aattcattga tctctagacc tacttgacgg cacactgaac
    25381 gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc tacaccatgt tctttcagag
    25441 ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag gaaagcaaga taaccaatca
    25501 aaaccatgtc tgactcatct ttgactgaaa attctccaac gacgcttgga ttggtctttt
    25561 gtgcgacacc aatcactcgt aagccatctt cattaagtga ctgtactttt gcaagtacgg
    25621 cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc aagatcaata aatttagaaa
    25681 tgttcagcat ttcttcaatc gcaccttttg tgatcatttg tgttttacca gctggatcag
    25741 caaccacaac actcatacga cgacgttcaa aatcaaaagg aatttcatcc actttttgat
    25801 aattgatctt atctgtatct aaagtttctg ttgcttcatc aatgatagcc acatctaaca
    25861 aatttttcag ccctgtttga tagtagctat ttagatacgc atggcgcaaa acacgatcat
    25921 cttcatttcc ttcacaatcc atatgatatt caagaatgat tttatcttgt gtcaatgtcc
    25981 ccgttttatc cgtacataac acatcgattg caccaaagtt ctggatggca ttcaaatttt
    26041 tgatcactgt tccttttttg gccatcacac ttgctccttt gaccaaattc gtcgtaacga
    26101 tcattggaag catttcaggt gttagaccaa ctgctacaga tagtccaaaa agaaaagctt
    26161 ctagccaatc cccttttgta agcccattga tgacgataac tgctggagcc atcaatgaca
    26221 taaatttgat cagtagcatg gatgttttat ggatgcctaa ttcaaagctt gattgaattg
    26281 gtttttcaga tagcgtttct gctacatgac caaatagtgt ttcatttcca acagaaacaa
    26341 tgattccttc cgcacttcca ctgatgacat tacttcccat aaagacaaga ttttcgtaac
    26401 ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg cttttctact ggatagcttt
    26461 cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc ggacaataaa cgcatatccg
    26521 ctggtatcat atcccctgct gaaagccgaa caagatctcc acagacgatt tcttccatag
    26581 gaatctctgt gtactttcca ttacgtttta cagctgccgt tactttcaca agtgatttca
    26641 gtttttcagc cgcttgattg gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga
    26701 taaccatagt gaagataatg gctacaccga aaagatcttt atctccagga gctgctaata
    26761 catattccgt gatgaatgaa ataattccta atgcgatcaa cactaatgta aatggcgtga
    26821 tgtatgcttt caatatttct acaatgaatg gtgttttttt gccatatgaa atcgcatttt
    26881 caccgtaatt ttctcttgaa cgttcgattt gttcttctgt caatccattt gctgatgcat
    26941 ggaatagttc aaataattct tgtttcgttt ttccagcaaa agctgcatag attttttgtg
    27001 tatctttctt tttttccatc tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct
    27061 gcttttggtc tttagctatt catcggatcg atagagaaaa aactgaaagg ggctgagaac
    27121 agatagataa tagccctggc ctttcctcta gatccttgtt tttcgaatga ctgtcatcca
    27181 tttgttctca tctcctttac taagtaattt taattcatga cttccaatca tttttcgaat
    27241 agttattttt ccaattagac cacctccttt ttatttttca ccgatgggga tttgcggcaa
    27301 caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa aaagcatatt aaaaaaagct
    27361 tctcagatct catcgttgag ttttgacact gtacaacgta aaaagcagat acctgctttt
    27421 agctacctta tgaaaagcct tatctcgaca attcctgttc tacccattgg cgtctctcga
    27481 cgtttttggg cagtagccta tgtttgtata ggagcctcac ctaacaaatc ttattcggtt
    27541 ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca
    27601 tacttttttt aaaatacaca cgttatattc ctgtctcacg cggttttatt tgtctcattt
    27661 tccacctccc gctataatag aaatatagta ggaaacgagg tgctgttatg gaaagaacac
    27721 ttgttattat caagccagac ggcgtgagac gccatttagt cggaagcatc atccaacgtt
    27781 ttgaagcaaa gggactggcc attgctgaaa tgaagtttga aactatgact cctgaattag
    27841 caaaagaaca ttatcagcac ttgactgagc gcagtttttt tgatgaactg atcgattaca
    27901 tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc
    27961 gtaagatggt tggagcaaca aaagctgccg atgctgttcc aggtacgatc cgaggagact
    28021 acgcgctgcc ggggactgaa aacatcattc acgcttctga ttcccgtgat gctgcggtaa
    28081 aagagatcgc ccgctttttc ccgccattcg atataaacaa accgtctgaa aaagcggcga
    28141 agtgatacaa attctaaact gctggtctag ttgctgggtt cagcaatggc cagcagtttt
    28201 ttctctaagg acctccgttg attctttcct aacatatatt cgagcactgt gaagaattct
    28261 tggtagtctt tcgtcttttc ttcccctaaa gcagcagaaa tcaatctttg ataataatct
    28321 gccatcaatg tttcatagat cactgtactt ctttctttat gtgctaagct ccgtttcata
    28381 tcttcattca cactacgtgc ctttttcaac cgaccatttt ctatataaaa cgaagtcagg
    28441 ttcattaaaa aagtaaagag atactgctca tgattgttca actgtgctaa tcgtcgcatt
    28501 ctttttttga ttttcaaata attgttctca atgaaccgat cactgaatat ccaaggaaaa
    28561 tcagctagaa tagatagttc aaattgacca tagtctggta tctgcatcaa gtattcttca
    28621 atgattcggc attcataacg taaaaattca aattctgtct cagatacttc tgttttttcg
    28681 accatgtgaa gattcaaaac caaataatac agatcttctg ttttcttaaa atgcctacgg
    28741 ttattgctga tctctttata aggatcaaag ctgtcttctt gtacccgatc tagcattcgt
    28801 ctttttttct ttaactttat tggttcatcc agctggtttc gcatccattc atattctgca
    28861 agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta caaatgacat ttttttcact
    28921 gaatttgtct caatcccgac tagagaagtc ttaaaacaga ttccttccgc taattgggac
    28981 tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt caccatcata cataatagtt
    29041 ctccttttta atttaaattt ttccacattc aataaataga tacgttaaaa aggaaaaata
    29101 cacgaaaaaa ataaaaaaat gtttttttca taaaaaatac caaccttcaa gcattagaat
    29161 gttgtctaac tactgaaggt tggtttgatt gtttattgat tatttttttg ctgttcaacg
    29221 tctgttcttg ttactggata taagttggtt gttgttttat taccattttt tgaaaagaca
    29281 cttgtcgaat gttcgccttt ttgcttttcg attgcttcat tacgttccgc ttcattttta
    29341 tagtcatatt ttttcggatc aatcgtctca aatccgtttg gcacataaaa ccgcaagaga
    29401 ttttcctgat tcagcttatc tgaaagactt aacgcagtct tgactttttc ctgatctttg
    29461 gagatttcct catcgattcc tgctgctttc gtatcgatga tttctcctgt ctgattattg
    29521 taagcttttc ctcctaggac ggtgtatttg ggtgtcacaa agttcccatt tcgaaaagca
    29581 actacttgat catgctgaga agataataag tctgtaccaa aatgaagata atctttatca
    29641 tcgattccca atagatgtag taatgtcggc aaaacatcga tttctccacc gtattcatga
    29701 ttgactgtcc ctttagtata accaggcata tggatcatca atggcactcg ttgcatctgt
    29761 gcgttgtcaa attcatccca tgtatctgga tctttaccaa gagcgcttgc taaatcttta
    29821 ttatctgtat tcgagatccc aaaatgatcc ccgtaaatca caaagattga attttggtaa
    29881 ataccagatt ttttcaaata cgtaaaaaat tgttccaatg attgatccag ataatgtgcc
    29941 gttcgaacat aattatctac tgtgctattg ccagtattca aagatggaaa gtcgaaattc
    30001 ttgtcatccg tataataagg cgtgtggttg gttacggaaa gaaatttcgt gtaaaacggc
    30061 tgttgtagat gctcaagata ttgggcagat tctctgaata aatctttatc taatatccca
    30121 taacctaacg tttcgtcgga ttcatcaaaa taagatcgat caaagaaatt gtcataacct
    30181 aagtttttat atacatgatc tctattccaa aaactagcta cattcccatg gaaaacagcg
    30241 cttgtatagc cttgcttttg tccaaggatc gctggtgcgg cttgaaagac attgtctgac
    30301 ccaagttctg tgaataatga gccttgaggt aaaccaaatg tgccagtttc caacatattt
    30361 tcagcatcac ttgtctttcc ctgtcctact tcatgaaaga aattgtcaaa gctgatcgtt
    30421 gcttgattct ggaaaatact atttaaaaac ggtgtgactt cttgtccgtc taccttcata
    30481 ttaatcaaaa actgctggaa actttctaaa tggagaacga tcacattttt ccctttagcg
    30541 atcccaaatg tttcgggatt tggctctgca taatgtttct ttgtataatc taaaacttca
    30601 tcaatcccat tactgcttgc atgcgcgcga acactgctag tcattcctgt tttgattccg
    30661 tcataaatag tatacgcatc gattcctaga tatttgacaa tatatgaacg gtcaaatgtt
    30721 cgctgcagca attgtggacg gttggcttcg ctcagcgcta gatttcctga aaaaataagc
    30781 cctgaaagac aggtaacagc gatagccatc ggtttgccga cagggttgct tttgataggt
    30841 attttcttga ccttcaacca aaccagtagc ccaatgaaca cagcgatatc cagccagtag
    30901 atgacatcat ggggcttcat caaagaaaag gagcttcctg acaatccttg cgacactttc
    30961 gaaaagccta atacagattt gactgtaatg aaatctgtga attcccgata aaaaataata
    31021 ttcaagtaca acaaaagcgt atttaaaata tcaatgatca aaagaatagc taacgctggc
    31081 tttggctttt taatatacaa agaaaggcca aaaaacaata aggtagtcgc aattgggttg
    31141 atccacaaga taaagtactg gatcgtacct tccacaccta atgaaaaatc tgcatagtag
    31201 gcaatgatcg tcttcagcca gaaaaagaaa acagctgtca aaacgattgc taaacgattt
    31261 tggcacaatt ttttaagttg tgtcacgtat atcacttctc tctattcaca ttttttctta
    31321 ttttgctcct gaaaaatgaa aatttcctaa aaaagttgtt atcttaagat ttttttattc
    31381 tttctaaaga ttattccgtc cacttttcac agcataaaac cgctgcgatg taagcgatta
    31441 aactaatgaa gagataaaag tcttgtggtg accgcttcac tccacggtat tcaaaagcca
    31501 gtttctcggt attcgtacaa aaaaagaggc tgtgacaaaa ttcttgtcgc agctccttat
    31561 ttcttgatgc aggacagctt tttcacaact tcttccttaa acggtgttct cctgccaact
    31621 cctcggccac aagtcacaat atttataaaa catgagaagc tgttttttga aattgttcct
    31681 tgtggctcag agttaaacgg caggctcaag acctcttgat acacggtgtt caatcagctg
    31741 accctcggta ttagattatg tcaggacgct aattttacaa cctcttggta aacggtgttc
    31801 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatataagga gctatttttt
    31861 gaaattgctc cttgtggctc agagttaaac ggcaggctca agacctcttg atacacggtg
    31921 ttcaatcagc tgaccttcgg tattagctca aaaaacggca aaatatgagg aactattttg
    31981 ccgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctg tttttcattg
    32041 tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg cttgattttc
    32101 ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc ctgtcgactc
    32161 gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt tccattccgc
    32221 taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga tcacttttcc
    32281 atttttcaaa cgggctactt tgtcgccatc atgagtcaaa agcaaaacgg tcaaatgctt
    32341 gtctttaaat tgatattgtc tgagagaaat cgcactaagt acgtcatcga tattaataaa
    32401 ttgattttct tcttttcctt ttggcaaata aatgtccaat tcgccttttt cgtaattggc
    32461 agttatttta tactccgccc accaataagt tgagaattct tttttgaaat tatctactga
    32521 ttctttgctt gctacgattt tttctgtttc tttctgagca agatctgctg tcatttttcc
    32581 tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa agagaagaaa
    32641 cggtcttttt ttcctcatgc tttcctccac tcctatttaa ttttgactat tctattatat
    32701 acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat tgaacttctt
    32761 tttcacaaaa tggtaagaat ggagaatttc acgcatttgc tctcaactat tctttcttaa
    32821 gcctcaaatc cgtttttatc tgatacgtct ttgaaccaat gacccgattt tttgattgtt
    32881 ctttttccat cttgtgctaa gtctactgaa ataaagccat atcgattctt atatgcattg
    32941 gtccacgacc agttatccat acatgtccac atatgatagc ctaaacaatt ggcaccttct
    33001 tgaatggctt gatggacata tttcagatga gattggacaa attcgatccg ataatcatcg
    33061 tggatcatgc catcttcttt gataaatcgt gcttcgtttt ctacacccat accgttttca
    33121 gaaataaagc agtcgatatt tccataatga tctcgtacat tgatcaaaat atcatagatt
    33181 ccttgttcat agatctccca tccgcgataa ggattcattt tacgaccagg catttcataa
    33241 ttgtcgaaat aatcctcagg cattggaccg tttttagtat cgattggtgt ttcttttgct
    33301 tttacccggc gtggctggta ataattgata cctaaaagat ctactgtatt ttctttgata
    33361 accgtcaaat cctctgcatg gatcacaggc atgagatcaa gttcttttac tatctcaatc
    33421 aattcacttg gaaactcgcc ttttacagct ggatcaagga atgaacgatt gaagaacgca
    33481 tctgctattt gcgctgcttt aacatcttca gggtttgatt cgtctcttgg atagcttggt
    33541 gtcagattca agatgatacc aataacacca ccttgctcca tttcatggta agatttgatg
    33601 gctaacgctg aagccaacgc ttcatgatag ccaacttgga tcgctttttt catatctgac
    33661 tcttctggat aatgccatcg atataaataa cctgcttcga ctggaacgat tggttcatta
    33721 tgagtaaacc atttttttac tttcgaacca aacaattcaa aacaactttt ggcatatttt
    33781 tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag atacatcggc
    33841 atatcgaaat gatacagatt cacaaagggt tcgataccgt tttcaatcaa ctcatcaaaa
    33901 taacgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc aggtatcaaa
    33961 cggctccatt ggatagatgt tcgaaatgaa ttatgcccag tctgtttcat caactgtaca
    34021 tcctcttgat acttcgtata aacttgcgag gttttttcag gtcctacttg atcaaagaac
    34081 ttttgcggtt cttttttgta ccaataatca aaaatgttct ctccttttcc atcgcctgaa
    34141 atccttcctt ctgtttgtgg gccacttgct gcagaacccc accaaaaatt tgctggaaat
    34201 tgatattttt tcatcaaagc acctcttttt cttttttagt tcgttttagt tgattccatg
    34261 tatgatattt tccatcacaa tcactttttc attttcttcc tcttcttttt tgattcgttt
    34321 taacagctgt tctgctgctc gttccccgat tttatctgtt ggtggcgtga tcgtgatttt
    34381 cggaaggata ctcagatcgt ttttatctaa ataatcatag tgactgaacg caatatcctt
    34441 cccgattctg acaccttctt gattgaaaca ttcgatggca ccaatcgaca tattgtaatt
    34501 gctgacaaaa atagcagttg cccctttttc taataatgct tttgccccgt aatagccgct
    34561 ttttctagaa tagtctcctg taaagacctc taattggatc gatggatcat tttggaccgc
    34621 ttctttggct cctgctaaac gttctcttgc tacgtagtct gtttgagtgg cagcaataaa
    34681 tccgattttt ttatgtcctt gttcaccatg atgagcgatc acttttgcta cactttctcg
    34741 atcattcact aatattgtat caatacctgc tggtccttct ggacaattca atgcgataac
    34801 tggaatactc atttttgtga gcatttcaaa tccagaccac tcttcttcgg ataagaagac
    34861 aatcagccca tcgatcacat gcttcatcag gtattcaatt ttttgttcaa ttttttctgg
    34921 ctgatcggca aacccgctta ataataagct atacccattt tcttcagcta ctctttcgat
    34981 ccctgagaca acttccgacc caaaacggct ggaaatcccg tttatcaaaa gtccaataga
    35041 gaagcttcga ttattcttca accctttagc aatgatgttt tctttatagt ccaacgctga
    35101 aatcgcttct gctatttttt tcatgttctt ttccttcagt tgctgaccat tcaagtaacg
    35161 ggaaactgtt cccactgaaa caccagccat cttagccaca tcacggatcg atgccataca
    35221 ttctcactcc tgccagttac atttgatttc tctttcttgt tgttcgatta tgatgaccgc
    35281 attctttgca tagtgttgct tgtcatcaat atccatataa ctaaagtctt cagtagagca
    35341 acttcctctc actattaatt atacttaaac aagtatttga accggttcat ttttaatata
    35401 gcattttttt aaaagattgc aacacttttt taaaacgctt tctgaaagtg gcggattttt
    35461 tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg tgcgtttttc
    35521 tcttccatcc gtttgaagtt ttacattcca atggtataat ttctttatcc tatcaaatgg
    35581 gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac ttttgacatt
    35641 gactggctgt gatttgtttg gcggctctga ccaatcctcg gacgaaacaa ccgattcttc
    35701 tgtcttaaaa gcggaaaaag cagataccac caacagtgta tatacgctaa aagtacgtaa
    35761 agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg aatggacacg
    35821 cacgtatcag acacaaggaa ctgattcttc tgaatcatct gaagaaaaaa cagtgctgac
    35881 agatatcaag atcactacca aagatgacga atatattatt tcaggaaaag aaaataaaaa
    35941 agaaacaaca gtcatattta caaaaatcgg taattaccgt ttgaaggatg aggatggcaa
    36001 catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct tcttttctgc
    36061 tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat agtcctgatt
    36121 attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa ggaagcttga
    36181 gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt gaatcttatc
    36241 ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa actcactatg
    36301 gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca tcagtaaaac
    36361 aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat ttcaaatatt
    36421 tgaatataaa ataaccaaac gatagaaatg cttattctac atcacgtagt aatttttctt
    36481 tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg ctggttatcc
    36541 gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg gatctgacgg
    36601 atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc atccacttgt
    36661 attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt caacgtttca
    36721 taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc acgttttttg
    36781 atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc aggccgtttt
    36841 tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc gtactgctgt
    36901 acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag gctatcttca
    36961 tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat tgctaaaaat
    37021 cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt tgaataaaag
    37081 agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc aatctctttt
    37141 gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat caaaatcact
    37201 gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac aggcaatgtt
    37261 tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag actttgtgta
    37321 atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt tttcaacata
    37381 gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc aaagtcaagg
    37441 atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta tacttcctgc
    37501 gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg agagtagaaa
    37561 cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg atcgatttgc
    37621 tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc tccttcaata
    37681 ataactgatt tttaatagat actgctcgta attcattgac cagttccaat ggattgttgg
    37741 aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca aaagtttcta
    37801 ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt ggcttgtcgg
    37861 gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat gtacctagac
    37921 ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt ctttcatcga
    37981 tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt tcaaaaagcc
    38041 cattcggata gatacctgtg acttttccgt tattttccag tactgtttta ctaatcactc
    38101 ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt gccatgtatt
    38161 ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg gaaccgcaaa
    38221 aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc attctacacg
    38281 tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata ggatagacgc
    38341 cactatatat agtatttaga aaaaaatcat ttttcagcca ttttatagat aaaacaaaaa
    38401 taaataacgt gttttcatcg atatgaaatg acttacttat agaagatact gggatttcgt
    38461 cctatatgcc aataacgtgt attattttaa acaacaaata cttgtttctt ttattaacga
    38521 aaaaatcatt ttgttctcgt gttatgtttc actgataaat gatgtttctc taaataaaaa
    38581 agccgacgaa ctaaacgatc aaaatttgat catttttgtt catcagcttc tcctaaaaat
    38641 ctttttgtta acgataaata gctggatttg taaaaatatc acttgaatcg tcactgctgg
    38701 aagaaaattc agtgataacc gcacctgttg cgcctgcctg gaactagtgt ttgatattgg
    38761 gctcgaacgt gtactgatct ctagcatgaa gaataatttc tttttttact gtataataac
    38821 tttctttcct ttttggaata gtacaggaaa gtttttttgt ttcctttcct tcgacataag
    38881 gtataccaga ccctttcgac aacgaaaggt tctcattttt ccttcatcgt catttcaagg
    38941 agggtgccga tgctctggac agtttcgatc gctcctaaac caaaatctgt atattcgatt
    39001 ctttctaact ctgattcttt cataccattc ctgtctcaag gtacatctat tgtacttttt
    39061 tcttgtaatc cattctgcct ctcctaacta attgtcaaat catcccaaga tatgtcttct
    39121 tcctcttttt cttcttggat gatttctgca ttcggatcta ctggtttttt caataataaa
    39181 atggttattc ctgtgacaaa agaaccagct aacaatgcac caatgaatgc ccatggtctg
    39241 gtcatcgtag cgactgcaaa cattcccgat gccggaatac gtctatctgc gccccaaaac
    39301 atgcttaatc cgccacctac tgtcccaccg actcccgttg ctatgacatt gcgtgccaag
    39361 tcgttcaata cgatagggaa acatccctct gtgatctgac agatccccat aggaaaagcg
    39421 gtcttcaatg tatcgatttc ttgacgattg agtatatttt tacgaatgat tttccctaag
    39481 aaataagcga tcgtcaagcc gaatggggtt accatcgaag ctaaaatcaa tacagtgatt
    39541 ggttcataga ttccttctga aaatcgtgcc acaccccctt atccagaata aatggtaatc
    39601 cagaaacagc cgtgtatctg cggtaataag cctaaccaaa cgaaaaatat gagaagctat
    39661 ttttgtttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttttttta
    39721 tttatgtttc ataatcaata tggtaaggat gcagctccgt atggtagacc gcctccgaat
    39781 attcaatcat ttccccaatt tgcgtagacg atttccgtat ccgtttgatg ccgatctgtg
    39841 cgtctgtttt cagaattcct tgttcttctt ctgttaaaaa tactgccgag aaatcatcag
    39901 aaaatgagtc aatagtatat ccttttttcg tcagcaaacg atacaaagat tctttgccaa
    39961 attcttctct gctgaattgc atcatttctt gtggaagata atgaacaaaa taaatataag
    40021 gctgattgtt caaataatat aagcgcttaa aacaaacagc ttgctcaccg aaaaaggaaa
    40081 acaaaggatc ttccttcttt atatggacca attgcaaatc cagcgtttct ttccggactt
    40141 catatcggga gttctccaag atttgcgtaa agcttgccgc tttggataat cgattgtaag
    40201 gacgattgct tagaaccgtc gtcccacgac cacttttctt tgcgacatat tcatctgatg
    40261 ccagcagttc gattgccttt cggacagtga ttttgctgac ttgaaacagt tcttctaatt
    40321 cactttctgt cggcaacatc gaaccgactg gatattttcc tgagaaaata tcttctttaa
    40381 ttttatctgc gacttccaag tatagtacac gtgtcctttt cataacgtca ccctttgctt
    40441 tactattact aaaagaatag cacataaaaa ataaaaaaag aacacaaatc acatgtgaga
    40501 agtgtgttct ttttcattca gctgctttaa ttacaagttt acgccatttg aggcaattac
    40561 ttctttatac caatcaaaag attttttctt tgatcgattc agtgtcccat ttccttcatc
    40621 gtctagatcc acatagataa agccgtaacg tttactcatt tctcctgttg atgcactgac
    40681 aagatcgata catccccaag gcgtatagcc catcaaatct acaccgtctg ctactgcatt
    40741 agccatcgct tcgatatgac gacgtaaata atcgatccga taatcatcaa taatcgtgcc
    40801 atcctcttcc atatgatcaa tggctccaag accgttttca acaatgaata atggtttctg
    40861 gtaacgattg tataattcat tcaatgcgat tctcagccct tctggatcga tctgccagcc
    40921 ccattcactt gcctcaagaa acggattttt tactcctccc agcaagttcc cttctgaagt
    40981 tgctgcttgt ggatcagtct cattgattgc cgtagacata taataactaa atccgatgta
    41041 gtccactgga tattctttta gcaactggaa gtctttttcc gtttgctcta atttttccgg
    41101 atcgactcca tattttttat gggtacgcgc tgtgtatgca gggtattctc cacgtacttg
    41161 gacatccgtg cagaaaaaat tgaattcttg gttttggatc attgttgctg cctgattgac
    41221 tggattggca tcgatgctgt aagttgttgc atagataatc atgcagccga tttggatatc
    41281 cgaacgcaat tcgtggccga tttttacggc tttgctactt gcaacgaatt gattatgcca
    41341 tgcttggaaa atattttggt actctcctgc tccattgctt tttactaatc cttgacttaa
    41401 agctggaaag tgaaaggccg aattgatttc attgaaagtc atccaatatt tgactttact
    41461 gctgtatctc tctaatactg tctgagcaaa tcgttcatag aagtcgatca gttttcgatt
    41521 tttccagcca ccgtattctt ttgccaaatg aagaggcatt tcgtaatgag aaatcgtgat
    41581 cacaggttca atatcatatt ttaagcactc atcaatcagc tgatcataaa attctaatcc
    41641 cgcttcgtta ggagtacctt cgtctccatt tggaaaaatt cgtgtccagg caatcgaaaa
    41701 acgataacat ttgaatccca tttccgcaaa caaggcaaga tcttctttga aacgatcgta
    41761 atgatcgatt cctcgatgat tcgggtaaat atatttttct tgatcaatcg tccagtcaaa
    41821 ttcttcactg ccgataatcg caaagcgctg cttacctcca ggcatagcat ctgcaacgga
    41881 tagtcctttt ccgtctcgat cataagcccc ttctaattga tttggcagca gttgctccac
    41941 cccataaaaa tttttctgaa aaacgtgatt gcatcctgat ttcctcctta ttttacaatt
    42001 gccagaacat cttcgtttgc aataatctct ttttggtcta attcaagtat atcggcaaat
    42061 gcatcagtat tcgtaatgac aactggagtg attacgctat agccagctgc tctaattgct
    42121 tcgatgtcaa aacgaatcaa taaatctcct gctttgacga gatccccttg tttgactaag
    42181 atctcaaagc cattcccatt taattctact gtatccatcc cgatatgaat caaaatctcc
    42241 gctccgtcag tcgttgtgat ccctacagca tgtccagttg gaaaaatagt cgtgatctct
    42301 ccgtctgcag gtgcaactag ttctcctact tctggatcaa tagcgatacc ttttcccatc
    42361 acgccagaag aaaaaacttg gtctggtact tcactcaatg gcagtacttt acccgttaaa
    42421 ggacttttta aggtatgccg tgctgaagta atcggttctc ctgcattcgc atgtttgttt
    42481 tctaattgat tgtcttgtgt ttgttcgtca aatcctaaaa tcaatgtacc tacgaatgca
    42541 agaacaaaag caattcctgt agctattact gctactgtca catttgactc tacaccatcc
    42601 accgtattaa tgaaagtcgg aatcgtcaat aagctgacca aactgctgga gaaagctttt
    42661 actccggaaa atccgataat agctccacca atacctccag aaatacaagc ggcaatgaat
    42721 ggtttcttca aaggtaatgt cacaccatac acagttggtt cagtgattcc aaataacgaa
    42781 gtcactgtag aagaaatccc taatgctcga agtttcgtat cttttgttcg caatgctaca
    42841 gctaaagcag ccccaccttg tgccaagatg gctggaagaa gcattggcat caatacatca
    42901 aacccatatt gttcgatatt caaaaacatg atcggtacga atccccaatg catgccgaac
    42961 atcacgaata cttgccataa acttcccatg attagacctg ctacgattgg actaaatcca
    43021 taaatcgagt tgaagagacc gcctaaagca ttaccggcaa ccgtcccgat tggtcctaaa
    43081 gcaagaaatg ttaatggaac catcactact aaaacgacaa gcggaactaa aatcatttgc
    43141 agaaattgcg gaatcacttt ttttacgaat ggctctaatt tactttgaac ccatacggct
    43201 agaatgattg gtatgacaga agaagtataa ccagatggtg aaagaatcac tgggatccca
    43261 aagaaactga tagcttctcc cgcaccagca actgctgtga tagctggata cacaagtgcc
    43321 atagcaattg ctactgctaa aaactggttg gctttgaact ttttagctgc tgtaaaagca
    43381 agcatgacag gtaagaaggt aaagacacca tctgctgcag caaacaatat ttgataagta
    43441 ccgctatcag ctgtcagcca gcccatcacc gttgccaatg ataagaatcc tttcaatacc
    43501 ccggcggctg ccatcgcccc tagaaaaggt gtaaatatac tagagataat atcgatcaga
    43561 cgattcaaaa tatttttgga ctcttctttt ccatcctccg aaactgcaag accttcctca
    43621 tccacgatcg attgataaac atctgctaca ttactgccaa tcactacttg atactgaccg
    43681 ccactttgta cgacttggat cacgtccggg tcttctttta acttttgcgt atctgctaaa
    43741 gattcatctt ttaatctgaa tcgcaaccgc gtagcacaat gtactaaact tttgacattc
    43801 ttttgtcctc ctaccgcttc ccatacacga cgaccaactg cttgattatc cattgttttt
    43861 tccttctttc ttttttattt aaaaaataaa aacccagatt gaaaaaaaca gtcacaaaca
    43921 ttcgtgaacc tattttttca atccaggttt tgcctgccat acagtaacaa tccttatgat
    43981 tggatgcttt ttttctcact tactactctt tgaatatgaa tcgttaaata aatctgctct
    44041 gcgatcccca tttcctgttt ttgcgtttgt cgaagatact cattgatccg ttcgacacaa
    44101 tgaaacgctt ttggatactt tccttgaact aatgcgtaga gaaattcatc ttgctcgtca
    44161 gatccttccc ctttgagata acgctgggca aaaaactgca gatgagtaat gatacgctga
    44221 taattcaaag attcttcatc gaacacgatg ccgaagaatt tactgatgat actcagtata
    44281 tctcttacca ttgtggtagc ggtcatcgtc acttgcatgt cttcgtttcc ttgttggcta
    44341 ttcacaaaat gaaaggcaat aaaaccggct tcatcctcag aaagttccac acccgtcttt
    44401 tgtttcacga acaaaagagc ctctttcgct gcttgatatt ctttcggata aaattttttt
    44461 gtttcaaata ataatggatt aggcagttgg atattttctt ttgctcgtgt gatcgcataa
    44521 tgaagatgat cggtcaatgt cagataaata ttaggcgaaa attcaacttt tagtttatct
    44581 tcagccagtt cgactacttg tttgacccat tcgatctcct ctgccggaat atcagccatc
    44641 agctgctgga attggttttt agcaaaagaa tctcttaaaa taaattcttt ttggaccgct
    44701 tctggcgaaa tctcgtcccc aatctttttt ttgaaaagct agtccttttc ccatacaaac
    44761 gacttcttcc ccgaatccat ttttcgaaat gacgacattg ttattcaaaa ttttatcaat
    44821 tttcattttg cttcgccctc tcaagaaaaa taaaaaaacc tagaattcgc ctactgtttc
    44881 ttagctgaat tctggttttg cctgccgtac agtaacaatc catttcttat tcaaaatcat
    44941 cataccatcg aatttttatt tatgcaagcc ttttcatata aaaaataaaa aaactggaac
    45001 acaagtctat ctagaaaaca accaaatcag agatgcacct ctttgcgttc ctcttttttt
    45061 cagcaaattg gtttttcctt atcgattcat atcctttggc tgtttttaat ctgtaacgac
    45121 ttatgtccca gtatcctaaa atgtttttgt ctctagattg aaagcagatc tcatatcatc
    45181 tgtcgcatct tttatcggtg taacactttc cagttctcct tgtacaacta gtcgcgtgac
    45241 ggcgtccatg tcgccacaag taattagtgt gacaatttct ttttcctcga cttcattcag
    45301 taattgggta tctgtaggag caactttttc tttaaaaaat gttttgtatg tatacacatt
    45361 ttctaaatct gtcaaataga tcttggcacc catctctaaa ttttccagcg gtgtaaacaa
    45421 caattcaggg ttcgttgcac gatggcttgc taaggcataa tttccttttc ccatttcttg
    45481 atctggagat aatgttcctg ctccgtaaag tagtgacgta ttatccaagc ctttgaaaat
    45541 cggtaaattg attgaaactg aagggactgc gatactagca atcactggta gatctgttcc
    45601 tctcatctgc gacctcatga ccccttctgt cgtcattggt tctaccgcat caaagtcaaa
    45661 actgacatct ttatcgttat tctttttcag atcttctttt gttacattcg ctatagcata
    45721 tttatctcct gtctctctga caagataatc cttgatctgt tcattgaaaa tcaaagctag
    45781 tcctactaat aacaagagga acaagaggat attgactagc catctttttg cttttccttt
    45841 ttttttcatt ttctctccct ccagactcat tttactgttt ttcttttgct ctttccaaca
    45901 gccgaatgac acatttctaa aactctgtgt atttttttcg tacacctgcg cttttttcta
    45961 caggatattt caaattattt ttgatcaatt tttcttcaat gatctcatct agatccagct
    46021 gaaggttgtc tgccatcatg tatgaataga tcaatacatc cgccagttct tctttgattc
    46081 gttctaattg tgtttctttt acttcttccg gcgttttcca ttgaaaaagt tctaacaatt
    46141 cagatgcttc caaagaaata gaaatagcca aatctttttc gttatgaaat tgtcgccaat
    46201 tacgttcatc tctaaactgg ttaacttttt ccatgctatt catttgcttg ccttcttcca
    46261 atcatttctt acttgattaa tcataccatt tttttacata aaaaatagat gatgctttta
    46321 aaaaaactcc tgttttcttt tatttaaaat aaatgtcata gaaatcagta atttaggtag
    46381 ccaccatgtg tatgcagtaa tgtgacttct tcccctgagg ctttttgttt ttcgatacgt
    46441 tcttctcccc aaacagacat cgctactaga atctctctta atgattttcc ttcttcggtg
    46501 agcgagtact ctactttagg cgggacttgg ttatagactt ttcgatggac aatgccatct
    46561 tgttctaact ctcttaactg ctgggtcagc attttttgtg tgatcgtagg gattttcttg
    46621 cttctccacg cctaaaataa gcgatattgc tcttcccttt atctggtgta agcaaagcag
    46681 ttagcatttt gacgatcgtg ggttccgctt ttcacttctt atctacttag tatcatgaag
    46741 tatactttcg tactaatact aagtagcgga agtgtcaact actttgttta actacatagc
    46801 aaaagaaaca gaagggtgac aacggtcatc acgcttctgt ttcttttgaa tatgctgtgt
    46861 tgttgtctct atctccgtgt agactttcct caagcttatc ctttgacagt aagtcagtag
    46921 gctgatttgc tctttctaga tgccagatac atttctacaa aaaaatagtg attctgtttt
    46981 gaaaattcga tttttagttc tgcattttcg taaacatcca acattttttt cgcactgtat
    47041 agacctaacc cgccattttt tttagaggtt tttcctcttt tgaccatctc cgatagcgtc
    47101 aaattcgtcg gatttgcctt gttcgctata ctaaaacgta atccctcttt ttcttccagc
    47161 agattgatcg taatcggact ctgatcggct acacgatgat cgaatgcgtt agaaataaaa
    47221 atagataaac agcgaataaa aacaaccaga tcaatcggga ttgttttcac ttcgacagga
    47281 ataactactt ggatcgggat tttctccgat tctgcttttt ctagaaattt taaaaagaca
    47341 cccctgacag ctggggatac aatatttgtt agctgttgta tttgcggttt ttcaaaaata
    47401 gctgcagaat gatcttctaa gatttctaaa aattccaacg tttctttttt attttcctga
    47461 agagtaattt ttaatgaaaa cagtagactt ttataatcat gtttgaattc ctctagatga
    47521 tggtaatatt cttcatttat ttgatacagt tgaagcgatt cctgatgttg tctttctttt
    47581 acttggaaac tgatcaacat aatggaaaaa aacaaagaaa aaaaactgat gactatcgac
    47641 aaaagagata gaagcagata attagatgag cggaagtgaa cacttcccaa taccaagtaa
    47701 atcacgcccc agcttaataa aatacaaata cctccaaaat agtgttcagt tggataagta
    47761 aaaagcaagt tgagaaatct cgtctgtttg tctatacttt ttactaatag atagaaaata
    47821 aatgcgataa gtagtgcaaa aaaacttgct gttccaagat gtctctttcc taaataatcc
    47881 atcagatcaa aaaccagaaa aaagctgagt ccagtgatag caaaaaaaag attactgatc
    47941 aaacagctga taaacaggtt tttcgtagta aaataaaaaa gaaacatcaa taataagcca
    48001 actaggtaag gaaagctcac tttagggata ggaaataaaa gtgtcaccac catcaaagct
    48061 ataaaaccaa agctgttttt tactagcagt tgtttcgaaa agaataaata gatacctaaa
    48121 tctaaaatca aagtaaaggt caaataaagt gtcactgttg cgaccatctt attcctccat
    48181 tgttttgttt caaaatgtac acatattttt ttaataggag cctgcatatg aaaaagtatt
    48241 ttatcataga agataaccag tttcacagcg aatggatcat caaagaattg gtcaatcatc
    48301 atatcgagaa tatcgtgcag ctcacttcta tctctcaaat caatcagttg atccagttgg
    48361 gtactattga taatcacgat gttttcttta tcgatatcga cttgaagcaa tcactcacag
    48421 gtattgatgt cgccgaacaa atacgtaaaa aaaatgcaca tagtaatatt gtttttttca
    48481 ccgcttatac gaattttgca gtagagatca tcaatcggaa aatccttccg attggatata
    48541 tcagaaaaga caggaattta tctgaacaaa ttcgtcaagt gctagaacag atcaattgga
    48601 aagaacagca aatcgtaaat gtcgataaaa tctttattga tcaaaaaggt ggaagtctcg
    48661 tcctgtatcc tcgggaaatt ttatatattg aaagtatcaa aggtttgaaa aaccaagtta
    48721 tggtcaaaac aactacagaa gataaattag tgaatacttc tttaaaacaa ttgaagacag
    48781 agttggaaaa gtatgcttac tttttactat taaaatctta ctgtatcaac ttatcgcaat
    48841 tgcagaaaat tgattctgct cattatgtgt tagaattttt caatggtgac tccttactcg
    48901 ttggtcgaaa gatctttgaa aagacaaagg aacgtttcca tgacttccaa aaaactgctt
    48961 cgagttgatg aataagagct gagacgtaag catagcagat ttcagggagt tggccttgaa
    49021 tcggctccac tccctgaagt cattgatttg attttacgct ttcccttctg taggagcatc
    49081 tgttaacgac catgtgaatg ttgaaacgta gttgtctacg gcataattgg atacaggaac
    49141 aaataattcc actgattgtc tcccttgttc cacattctct cctaatgaat aaatccatgt
    49201 gccaatccct tcatttgctc ctgcagttac tagatctact gcctgatctg ctgggatctc
    49261 aattccttca gcgccagtac cagaaatagt agatggcgtt ccactgctat tagttgcgct
    49321 atttcgaatc acggcgtttt tgaatttcac ctttgaacca gttagtttat tcgtatcaga
    49381 tgttgtacca ataaattctg ttcgtttgac tgatagtttc caaccggcat aaattccgct
    49441 tttatccgtt acttgtacga aattctgtac ttgtgttgct gtccctgtac tgtccgttcc
    49501 gttttgtaat gcagcatata ctgtttttgc tgtacctaac ttgattgttc caaaatcaag
    49561 atctggagct tggtcaatcg tcagtaatcc ggatgttccc tgatttggtt cgttaggtgt
    49621 aaacggcgta tcgggatcat ttggatcaac aggaggaact ggaacaacgt cttcaggatt
    49681 tgcggcatct ggttttgtta cagaaatcgt tccttctgta gtggctgagc tcttattctc
    49741 agcaaaaaca ctttgtgtac ataataatag acttccagct agaacggtac ttaatgcaat
    49801 tgtttttttc atttcttttt ccttctttct tttaaaataa ataatccagc aaataagcca
    49861 ataagaatga ctccaacaag tatcagccat cgccagtcaa atgtttctgg ctgatttttt
    49921 aaatcaatat ccttttcatt caattggtcc gctgcatcat cagttatttc aaaatcggta
    49981 tggagattcc agcgtttttc tttccaagca cccttcacaa ccgcttgata atgtcccgga
    50041 gctaattttt ttccttctaa tgggacaagt aaatcaaaat ttgtattggg tgctacatct
    50101 attttttctt tttttattga aagataaggc tcttttgttt cttttttgta gatttcaacc
    50161 gatgtagtca agccgaacat ataagaagca tttggatttt gaagattcgc cttaatcaca
    50221 tttcttccat cgctttgatc cgcataagtt ttttttaatt tcaaatttgg ggaagactct
    50281 ttttcgctat ttcgaatcaa gattgctttc acttgagaaa actgattgac gacaccaact
    50341 gtacttttgt tagaaacgtt ctcttttttt tcttgaaacg tgattcctcc tgcgaccaca
    50401 ccagccagct ctttttctgg agctttcagt gaaagtgtct cagtgaccgc actttgagga
    50461 gggacagtta tttctcccac tgtttttgct aagtttttca tatcataact taatgtttca
    50521 tcaaatttac tttttgattc tccatattcg atcacaccgt ttgaattcgt cgtagcggag
    50581 tggatcaatg tctcaactgt tactgattta tctgttcggt taaagaccgt gtaggggatt
    50641 tcctgtgttt tcccaggctc catccttaca tttatatatg ttttgttctt atcaatctga
    50701 ttagccggta attcaaaatc taccgcaaaa ttgaattcgg aggcatggac aggtgtctgc
    50761 caaaggatga atccaaggag aaggatcaga agtaaagagc agttgtttcg atgactttta
    50821 ttccatattc gattcatcaa tcattctcct tgatctttat tctgtcggcg catctgctaa
    50881 tgtccatgtc atctctgctg agtaattacc agctatctga ttttctttgg ttaagacaag
    50941 tttaaacgcc cgaccattat ttaaatattc tgaataatct gtttgcacat tcgttgaatt
    51001 ttgtcctctg acaatttcca gttcttcttc attcactaat tgatactcct cagctttctt
    51061 gaaatatagg acatcctgca tagaaaatcc tgcatcggtt gtgaaaggct gagataattt
    51121 tatcgttaag atccatggac tttttgtttc tcgctgatca taaatccata atttttcctg
    51181 tatatccatt tggccaaatg cttcttgcgt caacccagtt gctttgatct gaccacaatc
    51241 gagtttggaa ggggcaagaa atgagagacc accttctact acaatcttcg tagcaggtgc
    51301 aaaaggcggg gcatcatgat aaacaactgg cgtatcactt ggattgtgat tcccaatttc
    51361 atcgttggta cttggcttgt tctgcacacc cttagcagca gcttctcctg cttggtcgtt
    51421 taatacaatt tctaatacat ctcctgcaaa aagttgattt tctg
//
LOCUS       DAZULG010000007        50500 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000007 DAZULG010000000
VERSION     DAZULG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.007"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..493)
                     /gene="mreD"
                     /locus_tag="ACG9CY_000337"
     CDS             complement(<1..493)
                     /gene="mreD"
                     /locus_tag="ACG9CY_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS4936878.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKK"
     gene            complement(497..1351)
                     /gene="mreC"
                     /locus_tag="ACG9CY_000338"
     CDS             complement(497..1351)
                     /gene="mreC"
                     /locus_tag="ACG9CY_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS4936879.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(1506..2144)
                     /locus_tag="ACG9CY_000339"
     CDS             complement(1506..2144)
                     /locus_tag="ACG9CY_000339"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS4936880.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(2145..2795)
                     /gene="rpe"
                     /locus_tag="ACG9CY_000340"
     CDS             complement(2145..2795)
                     /gene="rpe"
                     /locus_tag="ACG9CY_000340"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4936881.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(2808..3707)
                     /gene="rsgA"
                     /locus_tag="ACG9CY_000341"
     CDS             complement(2808..3707)
                     /gene="rsgA"
                     /locus_tag="ACG9CY_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS4936882.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(3872..5941)
                     /gene="pknB"
                     /locus_tag="ACG9CY_000342"
     CDS             complement(3872..5941)
                     /gene="pknB"
                     /locus_tag="ACG9CY_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS4936883.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(5938..6678)
                     /locus_tag="ACG9CY_000343"
     CDS             complement(5938..6678)
                     /locus_tag="ACG9CY_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS4936884.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(6691..8049)
                     /gene="rsmB"
                     /locus_tag="ACG9CY_000344"
     CDS             complement(6691..8049)
                     /gene="rsmB"
                     /locus_tag="ACG9CY_000344"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS4936885.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(8049..8987)
                     /gene="fmt"
                     /locus_tag="ACG9CY_000345"
     CDS             complement(8049..8987)
                     /gene="fmt"
                     /locus_tag="ACG9CY_000345"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS4936886.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(8980..9471)
                     /gene="def"
                     /locus_tag="ACG9CY_000346"
     CDS             complement(8980..9471)
                     /gene="def"
                     /locus_tag="ACG9CY_000346"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4936887.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(9486..11894)
                     /gene="priA"
                     /locus_tag="ACG9CY_000347"
     CDS             complement(9486..11894)
                     /gene="priA"
                     /locus_tag="ACG9CY_000347"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS4936888.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(12147..12452)
                     /gene="rpoZ"
                     /locus_tag="ACG9CY_000348"
     CDS             complement(12147..12452)
                     /gene="rpoZ"
                     /locus_tag="ACG9CY_000348"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4936889.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(12457..13071)
                     /gene="gmk"
                     /locus_tag="ACG9CY_000349"
     CDS             complement(12457..13071)
                     /gene="gmk"
                     /locus_tag="ACG9CY_000349"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4936890.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(13558..13842)
                     /locus_tag="ACG9CY_000350"
     CDS             complement(13558..13842)
                     /locus_tag="ACG9CY_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936891.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(13860..14750)
                     /locus_tag="ACG9CY_000351"
     CDS             complement(13860..14750)
                     /locus_tag="ACG9CY_000351"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS4936892.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(14770..14988)
                     /locus_tag="ACG9CY_000352"
     CDS             complement(14770..14988)
                     /locus_tag="ACG9CY_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS4936893.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(14958..16658)
                     /locus_tag="ACG9CY_000353"
     CDS             complement(14958..16658)
                     /locus_tag="ACG9CY_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS4936894.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16925..17698
                     /locus_tag="ACG9CY_000354"
     CDS             16925..17698
                     /locus_tag="ACG9CY_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936895.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(17779..18348)
                     /gene="thiT"
                     /locus_tag="ACG9CY_000355"
     CDS             complement(17779..18348)
                     /gene="thiT"
                     /locus_tag="ACG9CY_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS4936896.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(18414..18516)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18522..19493)
                     /locus_tag="ACG9CY_000356"
     CDS             complement(18522..19493)
                     /locus_tag="ACG9CY_000356"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4936897.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(19587..20960)
                     /gene="glmU"
                     /locus_tag="ACG9CY_000357"
     CDS             complement(19587..20960)
                     /gene="glmU"
                     /locus_tag="ACG9CY_000357"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS4936898.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(21463..22281)
                     /gene="purR"
                     /locus_tag="ACG9CY_000358"
     CDS             complement(21463..22281)
                     /gene="purR"
                     /locus_tag="ACG9CY_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS4936899.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(22448..23272)
                     /locus_tag="ACG9CY_000359"
     CDS             complement(22448..23272)
                     /locus_tag="ACG9CY_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS4936900.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(23245..23925)
                     /locus_tag="ACG9CY_000360"
     CDS             complement(23245..23925)
                     /locus_tag="ACG9CY_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4936901.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(23948..24886)
                     /locus_tag="ACG9CY_000361"
     CDS             complement(23948..24886)
                     /locus_tag="ACG9CY_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4936902.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(25131..25982)
                     /gene="ispE"
                     /locus_tag="ACG9CY_000362"
     CDS             complement(25131..25982)
                     /gene="ispE"
                     /locus_tag="ACG9CY_000362"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS4936903.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(26136..28709)
                     /gene="mprF"
                     /locus_tag="ACG9CY_000363"
     CDS             complement(26136..28709)
                     /gene="mprF"
                     /locus_tag="ACG9CY_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4936904.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            28850..29656
                     /gene="proC"
                     /locus_tag="ACG9CY_000364"
     CDS             28850..29656
                     /gene="proC"
                     /locus_tag="ACG9CY_000364"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS4936905.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(29735..31291)
                     /gene="rny"
                     /locus_tag="ACG9CY_000365"
     CDS             complement(29735..31291)
                     /gene="rny"
                     /locus_tag="ACG9CY_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS4936906.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAEYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(31702..32751)
                     /gene="recA"
                     /locus_tag="ACG9CY_000366"
     CDS             complement(31702..32751)
                     /gene="recA"
                     /locus_tag="ACG9CY_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS4936907.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(32860..34101)
                     /locus_tag="ACG9CY_000367"
     CDS             complement(32860..34101)
                     /locus_tag="ACG9CY_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS4936908.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(34341..34919)
                     /gene="pgsA"
                     /locus_tag="ACG9CY_000368"
     CDS             complement(34341..34919)
                     /gene="pgsA"
                     /locus_tag="ACG9CY_000368"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS4936909.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(35128..36051)
                     /locus_tag="ACG9CY_000369"
     CDS             complement(35128..36051)
                     /locus_tag="ACG9CY_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS4936910.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(36790..38076)
                     /locus_tag="ACG9CY_000370"
     CDS             complement(36790..38076)
                     /locus_tag="ACG9CY_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4936911.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(38069..39337)
                     /locus_tag="ACG9CY_000371"
     CDS             complement(38069..39337)
                     /locus_tag="ACG9CY_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4936912.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            39589..40050
                     /gene="mscL"
                     /locus_tag="ACG9CY_000372"
     CDS             39589..40050
                     /gene="mscL"
                     /locus_tag="ACG9CY_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS4936913.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(40109..40555)
                     /locus_tag="ACG9CY_000373"
     CDS             complement(40109..40555)
                     /locus_tag="ACG9CY_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4936914.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(40648..40893)
                     /locus_tag="ACG9CY_000374"
     CDS             complement(40648..40893)
                     /locus_tag="ACG9CY_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS4936915.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(41008..41586)
                     /locus_tag="ACG9CY_000375"
     CDS             complement(41008..41586)
                     /locus_tag="ACG9CY_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4936916.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLCLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(41663..42193)
                     /locus_tag="ACG9CY_000376"
     CDS             complement(41663..42193)
                     /locus_tag="ACG9CY_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS4936917.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(42197..43057)
                     /gene="rlmB"
                     /locus_tag="ACG9CY_000377"
     CDS             complement(42197..43057)
                     /gene="rlmB"
                     /locus_tag="ACG9CY_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS4936918.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(43050..43445)
                     /locus_tag="ACG9CY_000378"
     CDS             complement(43050..43445)
                     /locus_tag="ACG9CY_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS4936919.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(43442..44854)
                     /gene="cysS"
                     /locus_tag="ACG9CY_000379"
     CDS             complement(43442..44854)
                     /gene="cysS"
                     /locus_tag="ACG9CY_000379"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS4936920.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(44851..45387)
                     /gene="epsC"
                     /locus_tag="ACG9CY_000380"
     CDS             complement(44851..45387)
                     /gene="epsC"
                     /locus_tag="ACG9CY_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS4936921.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(45473..45712)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(45709..47169)
                     /gene="gltX"
                     /locus_tag="ACG9CY_000381"
     CDS             complement(45709..47169)
                     /gene="gltX"
                     /locus_tag="ACG9CY_000381"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS4936922.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(47339..48496)
                     /locus_tag="ACG9CY_000382"
     CDS             complement(47339..48496)
                     /locus_tag="ACG9CY_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS4936923.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(48530..49900)
                     /gene="radA"
                     /locus_tag="ACG9CY_000383"
     CDS             complement(48530..49900)
                     /gene="radA"
                     /locus_tag="ACG9CY_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS4936924.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(49963..50490)
                     /locus_tag="ACG9CY_000384"
     CDS             complement(49963..50490)
                     /locus_tag="ACG9CY_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS4936925.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT        13781 a        11047 c         8982 g        16690 t
ORIGIN      
        1 atttcttaaa gggaaaaatg aataatacaa atataatcat gttcaacagc agtgtaggtc
       61 ctaaaaacct tgtaataaaa tacgtgttat ttactactgc taatttaaaa atcaactgga
      121 ccaccatcgt aaacaattca taacccgtga caaagatgat catactgaaa aattcagtaa
      181 agatgttgac atggataacg tctttcatta catacatcaa ccatacaatt aaagggagag
      241 ctactgcata gataccgata acgccaatat agtaagcgtc atagatagcc ccgagaacaa
      301 tcgtggtaat cagtaaataa cgtttttcaa acgccatact gcaacataat aatgccaata
      361 tcagaaagtg ggcatttgac atataggtgc ctttcgacca ctcccctagc attcttgtta
      421 aatgaccatc tatcaacatc aaaaagaaca aaacgattgg cagatagtac ttcattgttt
      481 ctttcttcaa catggactaa tcctccgcta gccgttggac aatcgtcaca actgacacgt
      541 catacatttc tgcataaggt ttcacataaa cttcccgatc cagcccataa ctatctggtt
      601 tcgttttaat aaccgtaccg atcggcaagt tagctggaga attccctcca agaccggatg
      661 tctggacaac atccccttct ttgatatccg tatcaccagt taattgggtc accactaagg
      721 catggagctt ttcatcatag tttttaagca aaccaaacgc ttcgccatta gccgaagata
      781 cccgtactgg aaaatgattg gagctttcat tagaggatga cagtaattcg attttagacg
      841 aagccgtatt gacctcgatc actcggccaa tcaatccttt ttgcgacatg actgccatat
      901 ttgcttcgat accgtcttta gaacccttgt ctacgatcag catgtcctgc caactgtcag
      961 gcgaacgagt aatgacattt gccgttactt tttcgtaact agtcagcgtc tgattcaact
     1021 caagttcttt tttcaacgct tcgatttctc tctcgtaatt cttgttttgc tgtgaaacgt
     1081 catcatagct atcgattttt tcttttagcc gctcattttc ggcataagtt gaaaaaagat
     1141 tatggacaga atctactcca tttcgtaccc accgtactgg ggcagagatt gttttatcaa
     1201 ccattgcaac actgtcatta acgatggatt ggaagaaatt gggctttcct tcattcgctc
     1261 gctgcgcagc ggtcacactt agtatcgtca ccacgatgat aacaataacc aaggtaataa
     1321 taatattttt attaggattg aacttcttca caaagacacc tcgaattaat tagactcttg
     1381 tgttcaattt tatcactaaa tgccgtgaaa caaaaggtaa gttgctcctc ttaagctttt
     1441 ttttagcaaa taatagattc tgtcattttt agcctgaaag acaaattttt ttatttgttt
     1501 atggatcaat ctttgctttg aataactgca atcataccat ctgaaaaaga aaattctgcg
     1561 gtatctgaaa gaaattcgtt acttgcataa gatgtcggat attctacttc tttattttcc
     1621 aatacatact tgctgccttt taatgtaaga ttcgagacgg gagtcaagca gcaataagct
     1681 aaatacttca tatctggtat ctgtcttact atatgctctc caggcaaata ataagacaaa
     1741 taattttgac aatctctgat aataagctga tgggcaaagg gtttgaagcg aggttccata
     1801 cctaaccaga gattggctaa aagatgatcc aatcttccgc ccgtagctcc aatcaaagtg
     1861 acttccgctt gcgggtaacg ttcaaatgtt tttaagatac ctaactgtgt atctgtatcg
     1921 tctttttctg caggggaacg ggtatactct ttggcttttg caaatacttc cttctgctct
     1981 tcgttcgaca atgaatcaaa atcacccact gccagatcta ctgggtagcc tgcttgaagc
     2041 aggtacagcg caccacggtc tatcccgaca taggcatcgt atttggacag cagagactgc
     2101 ggccagtttt gcggattacc tccagcagct aataaaattt tcatttagtc taatgcctct
     2161 tttaatcgtt cgatttgtcc gactggattt tcggctccat agatatatga tcctgcaacg
     2221 aagacatccg cccctgcatc tttacagatt tttgctgttt ctggaacgat tccgccatct
     2281 acttcgattg cataagtcca attgttctct ctacgtagat cagcgagttc cgtaattttt
     2341 tctactgttt cttcaataaa gctttgtccg ccgaacccag gattgacagt catgactagt
     2401 acttgatcag ccaaaggcaa aacatgtttg atcatggaga ctggtgttcc tggattgata
     2461 actactccag ctttaactcc taagcttttt gtcatttgaa gtgcccgatg aatatgagga
     2521 gttgcttcga cgtggacagt aatgatatct gctccagctt ttgcaaatgc tggaatgtag
     2581 ttttctggat caacgatcat cagatggacg tctaacggta atttagtaac aggacggatt
     2641 gcctgaacaa tatttggccc taatgtgata tttgggacaa atcggccatc cattacatct
     2701 acgtggatgt aatctgcgcc tcctttttcc actaactcaa tgtctctttg taaattggca
     2761 aaatctgcac tcaaaatgga tggtgctatc ttcattcttg tttcctccta ttttcgttta
     2821 ccttcttttt ttgcatacat tggttttctt ttttcgattt cttgtaagaa ttgcaaataa
     2881 ttgtcgtaac gtgtctgtgc gataccacct gtttctactc tatgttttac ttcgcagcca
     2941 ggttctttag catgcatgca ctctctaaat ttgcaatgga cagaagcttc tacaaattca
     3001 ggaaattgtc tcggcagttc tcctgcttca attgtcaaaa agtcaatcga gctaaatcct
     3061 ggtgtatctg ctactaaccc gccatagata ggaatcagtt ccacatgacg tgttgtatgc
     3121 cgtcctcttc caagtgcatc agaaatctct cctgttgcta aattcagttc cggtgatatt
     3181 tgattcagta gcgtcgattt tcctgctcca gactgaccca taaagaccga tagccgttct
     3241 gggaagtaac ggatcagttc tgtcgtatca cccgtatgat tgggtacaat caccggatag
     3301 cctattttag tataggtttg cttgatctct tcgatttttt ctgggtcgtc tgacaagtcg
     3361 gtctttgtca aaaaaataat cggttcgatc gactcgtatt ccaaggtaac tagaaagcgg
     3421 tcaagtagat tataagaaaa tgcaggctct acgatacttg tgacaatgat tccaagatca
     3481 acatttgtga ctggtgggcg aactaattga ttttttctcg gcaagatttc cagaagatag
     3541 ccgtctgttt gattttcact ctcaaagatc acctgatctc cgacaagcgg cgtgattttc
     3601 cgattccgga aatttcctct tcctcttgtt tgataggtct ctccgtttgc gtatacatag
     3661 taaaaaccgc tgattgcttt tctgatttgt cctttgataa gtgccacctc cgtttttgtt
     3721 tattttacca tacgaagagg aaagtaggtg ttattccatg caaacttctc aaagtatatg
     3781 gcttgaaaat tgatcaaaag agatagaaac acaaaaaaga gaatgcaaca aatcaatgtc
     3841 gcattccctt ctgatatatt ttattcagca cttattttga actttctgag gtgctagctg
     3901 atgaacttgt ggagctggaa gattcttctt ttgaagagtt gcttgtgcta ctggagctac
     3961 ttgagctgct agaactgtct gaagagtctt tttcagaact agaagacgct ggatctggtc
     4021 ctttagagta aattacgttc acaaccattc ctttggtaat tgtcgttcct gcccctggta
     4081 ctgtacgaat cactttatct ttatccaccg tgtttgaata ttcggaagtc tcatgcccga
     4141 tatattctgc accgacacta gctaaataac tttgtgcttc gctttttgta tagccggaga
     4201 tatcattcaa tttaggctct gttccttggc ttacgacaaa attgatgtaa gtttcttctg
     4261 ctacaacttc acccgaggct ggatcctgac tgatgatcga acctgatggt acagtgtcgc
     4321 tatagtcata agtagcacta atattattga tattgaatcc ttgtgtaaca agccaattac
     4381 gaacagtttc gtagttttct cctacatgac tactcaaagt cactgtttct ggtcctttgc
     4441 tgactgtgaa agtaatcgtg tctgttttag gatcgacttc tgtatcttct gatggagatt
     4501 gcgaaatgat cattccgctt tctacctctg aactgaattc ctcttctttt tgaatcaagt
     4561 cagcagaaaa ccctaattct ttcaaacttg cttcggcatc ttcataactt ttaccactgt
     4621 aatcatctaa tttgattttt ttcgtgccgc tactaatata cagagtgact tcgcgttttt
     4681 gcttgactgt cgttcccgct gccgggtctg tcttcactac attaccttct tcgattttgt
     4741 catctgcaat ttcttctgtt tttgttgcta cttctaattt cgcttttgcg agtgtttctc
     4801 tagcagaagc ttcagaaaga tcagaaacat caggaacaac aacttctcct cgcccgccag
     4861 aagcaaagaa tacagctgtt ccgattccta aaacagcaag aatagctaat aggatcagcc
     4921 accatttttt ctgtcctttc tttttaggat caggcagaac ttcttcctcc tctggtaagg
     4981 tttcgttttc tggcttgtct tttggcagag gcatagattt gaatgaatca ggcattggtg
     5041 catcttccgt aaggggtgtg ataacttttg tttcattatc catcacatga ggctgccatt
     5101 tttcttcgtt tgcacgagct ggtgataata ctgtagaaag atcatcagac atttcctcgg
     5161 ctgacttata acggtctgca ggttctttag ccgttgcccg taaaatcacg ttttccagtg
     5221 cttgtggcac atttggatca agtgctttta tagatggaag atcatcttga aaatgtttta
     5281 aagcaattgt tacagcagat tcaccatcaa acggtacgct acccgtcaac atctcataaa
     5341 ggatgatccc taaagcataa atatctgact gcttcgttgc catacttcct ctggcctgct
     5401 caggggacag ataatgaaca gatcctagca tcgtattcgt ctgcgtgatc gatgtttcag
     5461 ataaagcgat agcaatcccg aaatccgtga tttttacaac accttcatta tcgatcagta
     5521 cgttttgggg tttcaaatct cggtggatga tttgatggtt atgagctaaa gaaatagcag
     5581 aaaggatctg ctgcataata tttactgctg tttcataagg aacaggataa tgtgtttgga
     5641 tatatcgttt caagtccatt cctttgacgt attccatcac cagatactgc atgttgtctt
     5701 cttcaccaac atcgtaaaca ctaacaatgt ttggatgaac gagttctgtt gctgctaaag
     5761 cttctctctg aaatcgtcga atcgcagtct ggtcattttg aaagtcaaat cgtaagactt
     5821 taactgctac atcacgatcc aagattaaat cgtgcgccaa aaatacattt gccataccac
     5881 cgctaccgat atttccagtg atttgataac ggccatttaa ttttttgccc aactcgatca
     5941 tacactgtcc tccttatatt cgataagaag aacggtgata ttatccgctc cgcctgcttc
     6001 attggcacga tcaatcagct cagttacttt atgagacaat gggccttctt gattgatgat
     6061 cgttcctatt tcttcttcag acagcatgtt cgtcaagcca tctgaacata acagcagtcg
     6121 atcttttagt tgccaaagat gagtagaaac atctacttcg actgttccag gcatgccgac
     6181 agaacgtgtt aaaatatttt tccttggatg gttcactgcc atttcttcgc tgatttctcc
     6241 tgatttgact agttcattga ctaaagaatg atcctccgtc aattgcatga ttttatcttc
     6301 acgaatcaaa taagctctgc tatccccaac attggcaatc gtgaaccagt catctgacag
     6361 agcggccgct acgattgttg ttcccatacc gttatattcc ggctgttctt gtccacgctg
     6421 ataaatcagc atattttctt cttgaatcga ttgaatgaac cattgggcga ttttttcatc
     6481 gtcaacagct tcttgttttt cccaagcttc gcctagattc gttaccgcca tctgactggc
     6541 aatgtctccg gcacgatgac cacccatccc gtctgctaat acagcaagct tgagacctga
     6601 acggctggta aatacactgg catagtcttg atttgtgtta cgtcgtcttc ctacatctga
     6661 ttgatattca atctgcattt tttcacctca ttactttttg cgcaaacaac aaatgaaaaa
     6721 tccatctgtg taaaactgat gcggatacaa agtcaacatt ttctcttcga ttgatgattg
     6781 tagcatagga ttggtcaaaa catcaatcct ctcaaaatca gggtgtcttt ccaagaaagc
     6841 agaaacaacg tcttggtttt cttcttttaa gatcgtacaa gtgctataag tcagaatacc
     6901 ggaagacttt aaagttggtg cgcagctttc taaaatttcc aattggattt ttggtaaatt
     6961 cgctaattca tccggctgtt ttttataacg aatatctggt tttcgccgaa gcagcccaag
     7021 acctgaacaa ggcgcgtcta ctaaaatccg atcaaactgc tctgcttcga actgtttact
     7081 gacttctcta gcatccatct tttgagcgta gacagcagaa gagacaccta gacgtgctgc
     7141 attatcttca atgagtttga ctttgtgttc gtgtacatct aaagcgacca cttctccacc
     7201 gcatgacgga tctaaaaaag aagcaatatg cgtcgtctta cctccgggtg ctgcacaggc
     7261 atctaagaca tgatgttcag gctcgatttg catagacggc gctacaagca tcgaactttc
     7321 atcttggatc gtcatgacgc catcgtggaa caatgagcta gaagctaaat gccctttttt
     7381 cgctactata ccgtatggac tgacttggct tttttcagct gtgatctctt cttcttgcaa
     7441 aacagtgatg gcctgatctc tggaaagtcg gcgtgtatct actcgggcac tagcatggct
     7501 aggctgatat agcgataaac cgagcttttc agtttcttcg tatccgattt gatccaccag
     7561 tcgtttcgtc atccatagag gcaggctgat tttgacagaa agtctttcga taggatcagc
     7621 aatgccttgc agatctggtg ctccttgacg ctgataattc ctcaagacac catttacaaa
     7681 tttccctgtc ccaggatttc cttttacttt tgcgatttcg accgcctcgt ttaaaatagc
     7741 atgtgcaggg actttatcta aaaattctaa ttggtataca gaaagataaa gaagcatctt
     7801 gacccagtca tccacttttt ttgctttagc gatcaatggt gcaacgtaaa attccaatag
     7861 tcgctgatgg ctaatcgtac catagatcaa ttctgtaaat aaacccacat ctttttgatt
     7921 tagctcgctt tgtttgatca tctcattcaa caaaagattc gagtaggcgc cacccttgtt
     7981 cactcgctct aatgtcagta atgccatata tcgtactgat tttttcattt ttttagggat
     8041 ttttttcatt atttcacctg ctgaccaatc tgaacttgct gaccgcttcc gttaaggaaa
     8101 tcatgtacgg cctgttttcc tttaccagct ggctgtaatt cattgatggc taagacggtc
     8161 tgtttaccgc aagcaatcca tagatttttt ttgtctttcc ggatgatcgt accaggtgct
     8221 ttgtctgtag tttctgccaa tacttcaacc gataataatt tccagcgtat gccctcatag
     8281 gtcgtgaatg caattggcca aggacgcatt ccgcgtactt ggttgtcaat ttcttctgca
     8341 gttttatgcc aatcgattgc ttcttgttcc cgtgtgatgt ttggcgaaaa agttgcttct
     8401 gactcgtctt gaggtactgg tttttgtcca tccaaaatat tgggcaatgt ttctagcaat
     8461 agctttcttc ctaaaaggct aagcttttca aacatcgtcc ctacatcatc ttgtttagtg
     8521 attggtatgc tttcttgcgc atagattccg cctgcatcca tttttttgat catttccatg
     8581 atcgttacac cagtttcttt ctccccattc atgatggcat aatgaacagg cgcacctccc
     8641 cgatatttag gaagtaaaga agcatggaca ttgattgccc ctaattttgg tacttgcaat
     8701 aacttttcag gcaagaattg gccaaatgcc gcagtgacga tcacatcagg caccaattca
     8761 atgatttctt ccatttctgg cgaaccggag attttctctg gctgcagcac acggataccg
     8821 tgcttcagag ccgctttttt taccggtgtc ggcgtaatta ctcgtttccg cccaactgga
     8881 cggtcgggct gtgttaccac tgcctggatc tcgtaacctg attctatcag cccttcaaga
     8941 atcggcacag aaaaagcagg tgtgcccata aatactagct tagtcatcaa gatgttcctc
     9001 catatattct tcaagttctt cttccgggat ctgctgtatc attttctcaa tgaacaagat
     9061 accgtctaag tgatcgattt catgctggaa ggcacgggct aaatagccaa aagccgtcac
     9121 ttctatctct tctccgtctc gatcataata acgcacggtc acttcatctg ctcgcttgac
     9181 cgttccatag acatgcggaa tactgagaca accttctacg tcaaggcttt caccttttgc
     9241 ttcgatgatt tctgggttaa tcagttcaaa tttctcgcct tcatctactt cgatcacagc
     9301 aattctcttg ttttgtccta cttgaggagc agcgatccct attccgtcat tcgcaatcat
     9361 tgtctcatat aaattgtcta ataaagcgat tgtctcgtcc gtgattacat cgattggctg
     9421 agcagttctt tttaatttat cattagggtg gatcaatata ggatagcgca taatttggcc
     9481 ctttcttaaa taaagtttaa tggttcgttg tcaattgcca caaaaagacc ttgtcgctgt
     9541 tctttctggc ttccattcaa aatttcatcc aaaacatgag gaagattcgg ttcatgttta
     9601 tatttgatga ttagttgata ataatatcgg tttttcactc tcaaaatcat gctgggcgtt
     9661 ggccctaata gcaagctttg agggcttaac acttctttta gctgattggc aatctgaaag
     9721 attttctttg ccgctacttg ttcttcagga tggctggcgg ttattttcac agtaaagtaa
     9781 taaggaggat agcctccacg atgccgtaca tgcatttcct gctgataaaa ctgctcataa
     9841 ttttgttttt tagccaattc tatggcgtaa tgttgaggat tgaatgtttg gatcacgact
     9901 tcgcctgctt tttccgctcg ccctgctcgt cctgaaactt gggtaagcaa ttggaatgta
     9961 tgctcactag aacgaaaatc cggtaaattc agggaggtat cagcattcaa caccccaact
    10021 aatgtcactt ctggaaagtc caaaccttta gcaatcattt gtgtccctaa taatatatct
    10081 gcttcttttg caccgaattt ttgtaaaatc tgttcgtgtg ccccttttct tcgagtggta
    10141 tctacatcca tacgcaaaat cctagcttgc ggataaagtt ctcggagttc ctcttctact
    10201 ttttgagtac ctgttccata gtagcggatt ttattgccgc cacaattagg acagcgattg
    10261 ggaataggtt cttcatgtcc gcaataatgg cagcgcattg aacgtgtatc catatgcagt
    10321 gtaagagaga tatcgcaatt aggacatgga agaacaaatc cacagtccct acacatcaca
    10381 aaagacgaat atcctcgacg attgagcagt aggacagttt gttctttttt gtctaatcga
    10441 ttttgtattt tttcctgcaa attagcagaa aaagtagagg tccgatgatt ctgaaactct
    10501 tcacgcatat ctacgatctc aatagctgga agctgtgcag ctgctttggc tcggtggttt
    10561 aactgcaata actgataaac gcctttttga gctcgtgctc ttgattctaa agagggcgtc
    10621 gcgcttccta gaactattgg gcaatgatga tatttactgc gccaaattgc tagatctctc
    10681 gcatgataac gcggtgtctc atcttgtttg taacttgatt catgttcttc atcgataatg
    10741 atcagcccga tatttttcag cggggcaaaa atagcagatc gtgcgcctac tactacttgc
    10801 gcttcttccc gctcaatctt gcgccattca tcatattttt caccttggga aagtccgcta
    10861 tgtaagaccg caactgattc tccgaagcgt cctttgaatc gctcgaccat ttgcggagtc
    10921 aatgcgattt caggcactaa cataatagcc gttttatttt ctgacaaaac atcagcgatt
    10981 gcctgaagat atacttctgt tttcccgctg ccagtgattc cctccagcaa aaagacctga
    11041 tctttttgct gctgacctgc tgtgatgatt tgttcgacag catttttttg ttctgcattc
    11101 agttccaatg ccgtcgtttg gtcaaatact cggtctttat aagggtcgcg ataacgttct
    11161 gattctataa actccagcca gcccttttct tttccttgat tcaagacagc agtactgatt
    11221 cctttatttt tgaaataagc aattggttct tcttggctca gttcagataa acacaaaaga
    11281 agttcttttt gtttcttggc attttgccgt aattttgccc attcttcttc gatttgcttt
    11341 tcagttaaca aagaacggac caaacgaatc gtttttactt tatttcgtgt atggacttca
    11401 tatttgatat ctactaattg ctcttggcgc cagcgaatca attgagaaag gctgccttct
    11461 tcttctgctt ccttccagga aatcacttct cgctgatcaa acaatacctg aacttctggt
    11521 gtgtctgcca aaggatagat caatttatca tattctgctt tcatcacact cggaagcatc
    11581 gtttgtagac aagtgatttt gaatgcgtaa gtaatttttt tcatataatc agctaatgaa
    11641 agtaattctt cattgaccac cggagcaagg tctagcaaac gaataactgc cttcaaagat
    11701 ggattagctg attcttctga tttttggata gccataacaa atccttggac atgacgattg
    11761 ccattaccaa aagggacttc tactcgcatc ccgacttgca aagctgtttc catttcagag
    11821 ggaaccaaat aagtgaatgg ctgatcggtc tgcatagtag gtacgtccac aatgacttca
    11881 gctgttaatg tcatggttcg tttcctcctt tttttgtctt ttcttatcaa gcgacaaaaa
    11941 gttccttgcc tattttacta gagatcgatg gatttgtctt atcttctgcc aaatcctaac
    12001 aaaaaattaa cagcagataa gaaaaaaaag aactgtgtca aaaagttcag acacagcctt
    12061 ttctaccaga caataagtgg cgaatgtttg aaaaggaaac agctgggttt gcttttcttt
    12121 ttagatgttc gctctatttt tctttattac attttttgct cttcacggat acgtgattcc
    12181 aattcgtgtt gttcacgctc tttttgtgct tgacgttcct cttcttccat cttcaaacgt
    12241 tcacgtttta attcaggatg tggatcattg actacatttc ctgcatcgat ttcttctaaa
    12301 gcttgaccga cgctttttac tgaatcaaaa gaatctagag taggctgtgc acttgcatcc
    12361 aattcatgcg cgcgtttact tgctaaaatc actagagaat attttgaatt tacacgatct
    12421 aatagtgagt caattgaagg ttttaacatc ataagactac atctccttta acattttaat
    12481 atatttaccg attacgcgat ctactcgaaa atgttcactg gcaataatgt ttttgattcg
    12541 ttcaactgct agcggcactt catcatttac aacagcatag tcgtaaagag ccatcatttc
    12601 gatttcttct tttgcaactt tcattcgttt ttcgattact tcatctgcat ctgttcctcg
    12661 gcctacgatc cgtgatttta attccgctaa atctggcgga gtcaaaaaga tgaatacacc
    12721 atcgggcact ttttctttga cttgttttgc tccttggact tcaatctcca agaaaacatc
    12781 ttttccttca tccaatgtct tgttgacata tgacaaagga gttccataat aattcccgac
    12841 atattctgcg tattccagca tctctccagc ttcgatcatt gattcaaact cttctttgct
    12901 tcggaagaaa taatcaacgc cttcgacctc cccttcacgc attttacggg tagtcattga
    12961 gatcgaatac tgaaaatcat tatcctcact ctcaaaaatt gcttttcgta ctgtgccttt
    13021 tcctacaccg gatggaccag acaacacgat taataatcca cgctctgaca tgacgacgtc
    13081 ctttcaacta gtctttttgt acttatcgtt tattttgcac tttttttctg caactttcaa
    13141 ggttatcttt aattatagct agaaattggt aaaaaagcta ccctgtacca agaggtcgta
    13201 aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca taaacagctt ctcatgtttt
    13261 tcgtttggtt ggggttatta tcgcagatgc aagcttttga acgccgtgta taagaggtcg
    13321 tgaagaagcc gattagtttc gagtatgaag aaaaattttg tgaaaatagt ttctcacatt
    13381 tttgcataaa tttggcttaa taccgagaaa atggtttttg aacatcactt attcaaaata
    13441 aaaaaagatt gtgacacgaa ttttgtcaca acctttaaat gtattcctag agcaaagaag
    13501 ctaagcgaag aaatccaatt ctgtttattt attgtatttc tttgttcagc atccttgcta
    13561 aaagcccata ttggacaaag tattcactac acccatgatc tcatcttttc gagaacgtgc
    13621 tttttctgtc ggatcttcta cctcttctgt gtcaattcga acaataaatt tccgattcat
    13681 cgtcacgtaa gcaccgtcgt tgatcaaaat aaacagttcg ttctcattat agtctgcaca
    13741 ggcatctttc catacgtcaa agccgttttt ttcagaaggg atatcccgtt taaccacagt
    13801 ttttccactt aattcgtgga acgttacgtc ataatatttc attatctcta cctctttcgt
    13861 cattcaatat tttgtacttg ttctctgatt ttttctaaga tcgtcttaga ctggacgact
    13921 tgatttttaa tttcgattgc atttgatttg gaaccgatcg tgttgatttc ccgattcatt
    13981 tcttggatca ggaaatctaa ttcccgacct acaggacatt gtgcttcgat aagttcatct
    14041 agtttttgta catggatcgc cagacgatcc agttcctcgt gaatatctcc gcgttccaac
    14101 aagatggcta gctccgtcaa caaccgttgc tcatcgactt tcgctccaag aaattcctcc
    14161 agcttttttt gataccttat ttgaaattcc ttttcataga tttctacaaa actatcgagt
    14221 ttttttagta cttctttgaa ttcttcagta ttctttttca acactaaagc taaagcttga
    14281 ccttcttttt ctcgactcgc ctcaatagaa tcaagtgcgc ttttcaatgt atctagagct
    14341 aactgtgcca attcttccat gctctcttcc ggtttttctt ctaccgttac gaagttttcg
    14401 tccagcacga agcgttccgc catccgacta attgacactt gattttgact accgtatcgt
    14461 tgttgaattc cgtctgtcaa ttgagtgata agtgaatcaa tcaattccca gttgatgacg
    14521 atctgttttt cgtttttgcc aatataagct agattcacaa atacttcgat tcgaccgcgg
    14581 cttaactgct gtttaataag ctgacgaaaa tcgttttcaa caaagttcag gattttagga
    14641 cttcgtattt gaacatctag aaaacgctgg ttgacacttt ttatttcaat atctaactga
    14701 tacgcttttg tttcgcggat ggctttgcca aaaccagtca tacttttcat tcgacagggt
    14761 ccttcctcat tacatatttt ctattgcttt ttgcagttgt tcaaactctt cgttcgtcaa
    14821 tgtcaggcct ttccccattt tttcatggtc tggcgcccaa tctcgaagat cgaattttgg
    14881 cgggcgacca ttccagctga ctaggttcag ttctttacgc caccccttgt tgttttctga
    14941 caatatagcg atttcttcta cgatttcata tgaaaattct tgtgccatgg ttgttcctcc
    15001 ttgagataat ataaatatag gatcagtaat gtgtcaactg gttgcttaag caattcatgc
    15061 tgcaaacgat ccttgcgttt tccttgtagc agataatcaa aataaacagt tttcgtgcgc
    15121 tcattttgta ttttttgctg gtattttcga taccttcgcc attcttcagt acttaattga
    15181 ccaatttgtg ccaataaatc atccggcata ctttttatat cttgttggca gatcatttga
    15241 atcatcatgt ctacttgata gatttcattg ggcagtaaaa aatgtttttt tgctaaaagt
    15301 tctagcatcg ccaatccgca tgtctctggt acgaccctgt tcttatataa attacgcagt
    15361 ttttgccaaa attttatgct tttgaaaaaa gataattcta actgaatttc ttttgttaag
    15421 taagacaaaa atcctcgcca gtatgttctt gcaggtttaa cagaatgaaa tgtataccag
    15481 ttcccatagt aatagcctaa tcgttttttc atttccagat agcctaattg taaatttaat
    15541 tggttccttt ggctatcaaa aagcagaaag ctgcctagtg tccacaatgt ttgacatttc
    15601 caatggataa aaatgtctgg catacgaatt tttttcgctg ggcctggccc ttgcacatct
    15661 acgacaaatg cctctgttgc tcctttgtta atcgcaatat caataggtat tttgttgcga
    15721 tacccaccgt ctgcatattt attttttccg atctttcgat aagccatagc gggataaaat
    15781 gaagcagagg cgagtatcca atctggtatt tcttctgttt ttgtctgttg gatatcagtc
    15841 actacttccg ctagttctgg cagtttagta gacaccgtat ataaacgtat tttgggatta
    15901 tgtttgagta aggacaaatc caaattgtct ttgaccatct tttccaatgg agcagaagaa
    15961 accccgcctt caattagtgc tgttttcgtc atttggcgtc tttctcgata gaagcgttca
    16021 cgaaggtctt caactgcagc catttctggt aacgcaagca cttgtcttgt cgacagcttt
    16081 ttccataaat tcacagcttt tttcatatct ccttgtaaaa tcaatgcgcc gttcaacgca
    16141 cctaccgagg tacctgtgat aatctcaaag ttgatatcat gttccttcag cgcctgccac
    16201 acaccaatct gataagcacc gtgcgctcct cctccaccta aaacaagtgc tgtatgatag
    16261 accagctttt tatttaattg tgtggttcct tgatagccgt ttttatgaaa aatacgcaac
    16321 cattcttctg atagaggaga ttgaaataaa aaacaacagt tttctttaat aaaaaaacga
    16381 gcgcaagctt ccatataccg aaaaaatttt ttccattcaa aagtttcacc ttcaatcaac
    16441 agattattaa aaataacatc tcctctactt ttagcaatag taaaatagac ggtatatttt
    16501 ttcgatactg ctttaaaagc acattgcttt tttgcagaac gccaaaaagc gtctctactt
    16561 tcttgatgac tttgaaaaaa tggaaggggt tgttttgctt gctggatagg cgaatacagc
    16621 gacattttct gtgaaaagta aacttttttt atctccatcg cgctctcctt tttcttcaag
    16681 tataccagta cgtcataaaa aggtcatgag tctcgtattc gactttgtag attccataag
    16741 aaaacaattc ataaaacctc ctttaaataa aaaaatacaa aaatcacaaa aattaattga
    16801 aaatgagagt gttttcaatg aaaacgtgat tttgtaatgg aattttaaaa taaaagaaac
    16861 aagctttttt aatgcaaatg ttgtaaaata aaagagatta agaggaaata agaggaggaa
    16921 catgatgact gagtcagttt atgtacatat tgataccaca agtaatgctg ttttaacaaa
    16981 agggctgact gctactgact ttgcaaacag tatcgtgcat tttcctcaaa acttgctctt
    17041 gcttgatcca tctgcttctg ctggagaata tgaatcccat acagggctga aagtaatccg
    17101 tggtacagaa aatattcagc gtttcttttc ctcttctcgt aacaggcgtg gattcgatga
    17161 attgaagtgg atcgacttta ctgatttaac gatgctgaaa gaattgacac ctttagaaat
    17221 ttcagaatta ttatattttg gtcatatgaa aacacatctt cattcgccgt ttttttataa
    17281 actacagaat aattttgtat acttcgatct taatgatcag ctgaatcgga tttattatcg
    17341 ctatttagat gaattttata ggatttttgc tagtaaatta agttcgatct tgtcagataa
    17401 gatcaatgta aaaaaatctt ttttcaaaaa agaaacacct gttgaaaaaa tcagtgtgga
    17461 attattgaaa gaaatgcgta atttgatgca ggaagggatc attttttctt ttgatcaagt
    17521 tggtttgatc gatggtaaat atcgtatccc aatctatgtg attgaagatt atatttggaa
    17581 attaaaaggc acccggtatc gagaagagga aatcatcgct actttaatct actctgtcac
    17641 cgatcgacac tggcaattgg tagaagatgc cgatctttct tatctgccat tgaagtagat
    17701 ctatttctta tcaaaaaata aaaaggaaac aagaaactag gacatgattc tgtctcaagt
    17761 ttcttgcttc ttttttattt acagctcctg tcgtttatct ggcacaaaaa tcgaaggagt
    17821 cgtttgttgg atccctaata aaacgatgat cgttacaata gctgtactca acccgctggc
    17881 accattcatt acgaaagaaa agagccaagg attcatcccc catagtgcat acgctcccca
    17941 gaaaaccact cctgcaataa aatgccaaaa atatctggaa agtgcgccaa tcaatgcacc
    18001 aaacacgatg ctttttctaa tttgtttcgt attatcgtct acatagcctt tttggacatt
    18061 tgaagccatg agccctgctg cgcctgcaaa agtataggca atcgggtact caatcaacac
    18121 ttgaacgaca cttaaataat aaacttgtcc caacggaaaa tgtaggatgc cccaaatgaa
    18181 acctgccagc atccctgcct ttgtccctct tcttaacgca aaaagagtca atggaatctg
    18241 ccctaatgaa atagaaaaac tgctgccgat ctggatcgga atcaacgaca acaccatcgc
    18301 taacgctgca acaatcgttc cttctaccca aactctattt tttcccataa aaaaacttcc
    18361 tcctttgaat aaaaaagtca caaaggagga agcagctttc gaaaatagtc aaacagctgt
    18421 ttattcacaa ttcctacgct cgcataaccg atacaggtac gaagggttta gaaatagatt
    18481 caatctcagc ccaattggac tcccctttgt gagtttattt attatttatt ttttgtttca
    18541 aacaatggac taactggttt gttttcatgg atacgtttga ttgcatctcc cattagtccg
    18601 ccaacactta cttcatcgat tttgtcgatt ttacgatctt ctggaagata gatcgagtct
    18661 gtaacaacta gacgctcgat agctgaatct tctattcttt gtaaagcagg gccggataat
    18721 actggatgtg tacatgaagc atacacacta gttgcaccag cttctttcaa agcattggca
    18781 gccaatgtga tcgtacctgc tgtatcgatc atatcatcga tcagtacaca agttttgcct
    18841 ttgacttgtc caatgatatt cattacttct gcaacatttg cttttggacg gcgtttgtct
    18901 atgatcgcaa tcggagattt caaaaattcc gctaatttac gtgcccgtgt gacaccacca
    18961 tggtctggtg aaacaacgac aacatcatcg cctttgattc ctttttctaa gaaataattg
    19021 gcaataagtg gcgcacccat caaatgatcg actggaatat caaagaaacc ttggatttgg
    19081 actgcatgaa ggtccaaagt cagcattctt gttgcaccag ctttttgaat catgtttgct
    19141 actaatttcg ctgtgattgg ttcccgtgca cgtgctttac ggtcttgacg cgcatagcca
    19201 taataaggca tgactacgtt gattgttttt gcacttgctc gtttcagtgc gtcaatcatg
    19261 atcagcaact ccatcaagtt atcattgact gggctgctcg ttgattgaat aacatagaca
    19321 tgcgatccac gaatactttc ttcaatgttt acttgaattt ctccatcact aaattgagta
    19381 acagatgatt ttcctaactc gacgccgact gctgctgcaa ttttctctgc taatgggcga
    19441 ttggaattta acgcaaaaat ttttaatctt gggtcaaagt aatgttttga catggggacc
    19501 tccactttct ttttcacaac tctagaattc tacctaataa atagtagtca tattttttaa
    19561 ataaatcaag tgattttgca aaagtcttag tttagataag ggagtttttt tgcatatcct
    19621 tctttattga cttgtcttgc tctcgcaatt gccagcgcat attctggaat attgtctgta
    19681 attgttgacc cggcagcaat cgaactgttt ttagctacct cgacaggtcc gatgatattc
    19741 gtgctagaac caataaagct gtggtcgccg acagtcgtat gatgtttgtt ctttccatca
    19801 tagttgacga agacaacacc acatccgaca ttgatttctt ctcccagcgt agcatcacca
    19861 acataagtca agtgaccgac tttagttcct ttgccgattt gagcattttt cacttccaca
    19921 aagtttccga tatggacgtt ttcgccaatt tcagcttttg gtcgtaaatg tgcataaggt
    19981 cccacatctg catgtttctt gacatggctg ctttcaatga ctgagttttc aatgacgacg
    20041 tgatcctcaa tcctactgtc tacgattttt gaatgtgacc cgatgacaca atcagaacca
    20101 atgactgtat ttccttggat ttgtacacct gcttcgatca atgtgtctgg accgatttct
    20161 acgcctgcat cgatgtatgt cgttgctgaa tcgacaaagc ttacgccatt gaccatatgc
    20221 atctggttaa ttcgctttct catgatctca tttgctttag ctaacgcgat ccgatcattg
    20281 actcccattg attcttcaaa atcatccgtt tgataagcag caactgtatg gccttcttct
    20341 tttaagattt cgatgatatc tgttagatag tattcacctt gagcattgtt tgttccaact
    20401 ttgcttaacg catcgaacaa agcttcgttg tcaaaacagt aagttcctgt attgatttct
    20461 tgtacaagag cttcttctgg tgttgcatct ttttgttcca cgattttttc tacgatccca
    20521 atatggtcac ggatgattcg tccatatcct gttgggtttt ctgcttgtgc cgtcaagatc
    20581 gttgcacttg cattttttcc ttggtgatat tcaaaaagat tgttcagcgt ttcagtagtc
    20641 aataatggtg tatcgccact gataactaac gttgttcctt tttttccttc caagaaagaa
    20701 gcagcttgca aaacagcatg tcctgttccc aattgttctg cttgtaacgc atatttactg
    20761 cgttctccta gttgtgcttt aacttgttct gctccgtgtc caacgatcgt aatcacttca
    20821 tctggtttcg tttcactgac ccgattaatg atatgttcta ccataggttt accagaaacg
    20881 ggatgtaaaa ctttatataa ttttgacttc atgcgggtac ctttccctgc cgccaaaata
    20941 attgcataac gtgcgtccaa ctgcgtcact cctaaatata gtttgctgat ttcagtttat
    21001 cccagtatac ccattttttc aacaaaacaa gtgattttct tttatcccag cgttttctgg
    21061 ataaaaaaat agatagacca taacgggcct atctatttgg taaccggtgc tctcctgcca
    21121 actcctcggt cacaagtcac actactcata aaacatgaaa agctgttttt tgaaatcgtt
    21181 tcttgtgact tagagttaaa cggtaggctt acaacctctt cattaaacgt ttttcaatca
    21241 gctgaccttc ggtattagct caataaacgg caaaatatga ggagcaattt ttccgtttat
    21301 tgagctaata ctcaggacag gacgctgatt tcacaacctc tagataaacg gtgttcaaaa
    21361 gccagtttct cggtattaag ccaaatttat gaaaaaatgt ggaaagccat tttcacaaaa
    21421 tttttcttac tactcgaaac taagcggctt tttcacaacc tcttattctg aaaaataatt
    21481 tcctggcaca accgtaatat ttttcgtttg agtatctaca tctttgacat aaagcagtga
    21541 cgtataatca tcgatggcgc gatgaccgtt gaattttgat tcggcaaata cagtgatgcc
    21601 aacaagttct gattcgaatt cttcaatcaa gctcttcatt ccgttgactg ttccgccgcc
    21661 tttcatgaaa tcatccacta caaggacacg tgaaccacgt ttcaaacttc tcttagacag
    21721 ttccatcttt tcaattcttt cagaagatcc agacacatag ttgacactta ctgttgatcc
    21781 ttctgttatt tttgagtccc ggcgtacaat aacgaatggc gcattcagat aataggacac
    21841 agcttgggca ataggtacgc cttttgtggc tacagtcatt acagcatcga ttttttcacc
    21901 aaggtattta gaagcaatga ttcttccaac ttgacgcagc agttcaggtt ctcctaacaa
    21961 atcagagaga taaacatatc ctcctggaag cagacggtct tgttctgata gacgttctgc
    22021 tagagattca atatattttt tcgcctcttc atatgaaatt tcagggatga acaatacacc
    22081 acctgcagca cccgggattg tttccaaggt tccataccct cgttctttaa atgttttctt
    22141 gatgattccc aaatcttcac tgatagaaga tttagcagat tcataacgtt cggcaaagct
    22201 tgttaaggaa atcaattcat gcgggtgatt caataaatac tgtgtcatgt caactagacg
    22261 ttcactgcga cgtactttca cctattccaa ctccttttaa gaaattttat cgttttacat
    22321 tatataagtt tttcttcaaa aattcgagca ttttatgaaa aaataagaaa atagttcggt
    22381 atttattaaa ataaaaagaa gcctataaaa aatttttcgt ttttataagg cttcttttct
    22441 ttataattta gttatttttt cttctgaagg caaccaatat tcgtttgatg atatttacaa
    22501 tcaggaacaa agcaataaag actaatgtga ttgttgctcc tggaggtgta tctagataaa
    22561 acgagctagt tagtcctgac agcatcccta gaaatcctac acaaacactg atagcaatca
    22621 cgacattgaa gcttttgcct aatcgcatac cgattgctgc gggaagaacc atgattgcgg
    22681 atatcaaaag tgcaccggca atcgggatca tcacagcaat agccacacca gtaatcacat
    22741 tgaacatcat cgacatcaaa tgaacgggta gcccatccac gtgggcggta tcttcatcaa
    22801 aagtcagaac atacatcggc cgtttgaata aaaagaacaa tataccgatg gcaacgaata
    22861 agattcctaa aaagatgact tgctcccatg taatggtcac gatcgaacca aacagatagg
    22921 attggatact tgttgctgaa ttccctccgc tcaaattcat caatactaac gctagcgcca
    22981 aaccaccagc cattaaaatg gcaatggaga tttctgaata ggttcggtag atcgttcgca
    23041 gatactccaa tatcacagct gctaatacaa cgactagcat ggtcgtaagt gtaggattaa
    23101 ggttcaaaaa gaatcctaac gctacccctg ctaaagagac atgagaaagc gtatcagcca
    23161 tcagcgattg tcttctgatc actaaaaaca cacctagcat cggggcgatt ccggcaatga
    23221 agatagcggc aagaaacgct cttctcatga attcatatga aagcaacgcc attgagaatc
    23281 ctcctttctc accagtcgga tctgtctgtc cgcatactgt ttgatatctt catgatcatg
    23341 ggtgatcatt agaactgatt tcccatggac atgcgcacta tgtcggagaa gacgataaaa
    23401 ttcatttctg gattcttcat ccatacctgt tgttggttca tcaagaacaa ataagtcagg
    23461 gtcagtagca aaaatacgcg ctaaactgat tcgctgcttt tgtccgccag acaattcacc
    23521 tattcgccga tttcgcatat cccacatccc tacagcttct aaagcacgct gtacatgttg
    23581 atgatctttt tcagagagac gcttgaacca gcggcctctt ggatatcttc ctgaacggac
    23641 taattcgatc accgtactag gaaaacctgc attgaaagat gcaacttgtt gaggaatata
    23701 accgatactt aatttttttc cttcttgatt tttctcagca atctttatca ttccttttga
    23761 aggtttcagt aatcctaacg atgctttcac caaggttgac ttggctgctc cattttctcc
    23821 agttaaaatg acaaattcac ccgcatctac atgataagag acatcttcta gtacaggttc
    23881 atcatcatag taaaaggtca aatcagatat ttcgatataa cgcaccttac aacctccatt
    23941 ctaaaactta attgatactt tcttttaaag ctttcagatt tttttccatg acagaaatat
    24001 aattttcgcc tgctttgatt tgttcattag tcaagctttc taaaggattc aacacttcta
    24061 gcttcactcc tgtttcattc gacaatgtct cagctacctt cgaagaggca ttttcttcaa
    24121 aataaatcac tttgatgttg tttttcttca caaattcttt tagctcagct aagcgacttg
    24181 gtgtgggttc ctgatctggt gaaacgcccg aaatcgctac ttgttttaag tcgtattgat
    24241 tcgctaaata agcaaaagca gcatgctgag tgacaaatgt gcggtttgtt gcatctttta
    24301 gctcatcgga gtatttttga tccagtgctt caagcttttt gatataagca tcggcattct
    24361 tcgtaaaagt ttccttttta tctggatatt tttcgcctaa ttcttcagca atcgttctga
    24421 cttcttttat agctaatacc ggatcgagcc aaacatgtgg atcgtattca tgtgaatgac
    24481 catgcccttc ttctgattct tcatgttcgt ctgcttcttg cgattctaaa cggtcgattc
    24541 ctttagctgc ttcgatcatc aaggtctgtt tgctatccac tgaatccttt aacgaatcca
    24601 caaaaaattc catgtctgaa ctattataca cgaaaacatc tgcatcggaa atttctgctc
    24661 tttcttttgc acttggttcg aaatcatgcg gctccgttcc agcagggatc aaaagtttca
    24721 cgtttccttc atcccctacg atctcttttg taaaatcata catcggataa aaagtcgtaa
    24781 cgatcgtcaa atcctctttt ttgtctgctt ccttgttcgt atttccacat gctgcaaaca
    24841 gtaaagcacc tatcattacc gtagctgtca aaagatattt tttcatttat atgctccttt
    24901 caatgaacaa atcggaattt tacgatttat taacgtgaac taatgtatca aaataaaaaa
    24961 acacagtcaa tagaaatcca tcaatttctg atttaagcat gtatattctt gattaaatcg
    25021 taaaatggat gagttgctgc tcatatctag ccatttcact acaataaaaa actgaagaat
    25081 accattttag gattcctcag tttttcatca cctattttac ttcttatatt ttattgcagg
    25141 gttcgtacta aataaacttc ctcacaaaaa ccttttaacc cattatatac tctctgcgct
    25201 cttgaataat gttgacacaa ggcaaaaacg gttggtccgc ttccactcat caatgcagca
    25261 tctgctccgt acttgatcat acgctctttg atttgatgga tgactggatg ccgctttccc
    25321 gttatatctt ctaaactgtt tcccattttt ttgatcatct gctgataatc ttgctctcgg
    25381 atggcttcag caagtccgtg gatgtcttga tgttggatgg cttgcgtatc tacttctcta
    25441 aacactgtac gtgtagagac actcattctt ggtttgacca acacaaccca gcactgtggc
    25501 atagaaggta atacttggat ttcttcccct ttgccaccga caaatgccgt acttccgtag
    25561 acacagtaag gaacatctgt accgacttcc aaaccgattt ctgccagttc ttctaacgaa
    25621 cagcccagat tccataattg gttgatccct cttagagcag ctgcacagtc tgtacttcct
    25681 ccgcctaatc cagctgctac tggaatattc ttcttaacaa agatcttgac accggttttg
    25741 atattgtatt tattttttag caataaagca gcttgataaa cattgttccg ttcatctacc
    25801 ggtaaaaatg ctttatttgt ttctacaatg attttgtctt ctgttatttc ttcaaaaaat
    25861 agatgatctg ccaagtctac gctcgacatc accatttcca actcatgata cccatcttgc
    25921 cgtctatata atacatccag tcctaaattt atcttggctg gcgctttttc aatcaattcc
    25981 atcctctcac ccattcgaaa agtctaagtc gcaagtattc tatcataggg aaaaataaaa
    26041 gtatagctta tgtctgccta gtggttgatt ttgtcatcgt ttcaacagct ttcacagaat
    26101 tttctcttca taatccaaac aagttctagt gattcttata attctgtgag cacttcttct
    26161 gccaaactcg gaacatttcc tttttctttt cctatcagac tgagtagctg catggtagca
    26221 aatccgaaat tacttccttt cctataagca aaataacgtc cttcccaatg atcgacatat
    26281 tttaatttct tttcataagc agactggaag gaatagaaaa aattaccata attatatacg
    26341 atatgcatga ctttttcttg aaagaaagaa aaatctgttt ctccaacatt agctaatgga
    26401 gctaagccta gattggcttc cgagtagccc attttctcgt atacaataaa cagattggct
    26461 aacagatagt cagctaattc ctcagattct ttaggtaaga cccgcaacaa atcatatccc
    26521 gcctgcttct tcgtcatcgg ctgagcggtg atgaagccaa cgactttttg atctttacgg
    26581 acaataccga caccgctaga taatacatat gatttatcaa atcgtcccac agagaagtat
    26641 ttttctcgtg caccttgaag ccatttttgt gaaacttcat ttacagcttg atgtaattct
    26701 tccggcagtt gatcgtacca agtaaaatgg tatccttctt tcatcaaacg ttgatattca
    26761 agttgctgac tcagccgaga caagtcttct gttgctagat caacaatccc tgcttcacct
    26821 actttcgtga agtgaaaacc caattcatga agcatcacta cgtagctttc tgatatctta
    26881 taaaacgcaa gctgatagcc taaacaatct gcttgtttca taaactgatt cgttgctgcc
    26941 cgcatcattt taggattgcc aactggatct cctaaaacaa agcatttgtt tcctttgatc
    27001 tgataaccaa aaacgacttg atcttggtcg tcttcttggt aataaaaata actgtaacct
    27061 gacaagcgaa gctgatggct tgtagctgtg cctccgtatg tttccaataa acggtcaaaa
    27121 cggtcttttt cgtactcact gccaagcttc gtagtatctt cagctaaata ttgataaagc
    27181 gccactaaag ctagcaatga gatgcccaaa ccaataagcc ctgagaacca cacatcatct
    27241 gaaggaaaca aaataaaatg atttcctgtt attcgaccga ctaaaaagcg tccgctgtga
    27301 tagcctgcaa ttgcgtaaac gataaacaaa aagccgaaca agctagcatc aaagattata
    27361 gctccccaag aataaacgaa ttttgttcga taaaattctt ttctggataa ccacacagct
    27421 aacagaatca agacatatac caatatcaac tgccaagata acgtacgtgt gattgtattt
    27481 aaaataccaa aagatagtaa agcaatcgta ggcagaaatg cctttttgac ttttactgaa
    27541 agtgctctag caaggccgag taataaaaag ccaaccaata gattcactgt ctgatctaaa
    27601 aaattgaatg agaacggcaa taatacttga aatagccgac tgattgcaga cagattagtg
    27661 attgttgata gaagtaccat catgatcccc gcaaaataga cagcacttac gagcacgata
    27721 tgcgcaacct tttggaaaaa cagtcgcgga atgttatcta aaaatcgatt gattttgact
    27781 cctgtatgtg tgatgaacaa tagaagtccc gtcataaaag gtagaacata ataaaacaac
    27841 cgataatata atagccaaac tactgctaca tcttgccgaa cagacaattg actcattcct
    27901 aagatcatca aaacgtcaaa agtccccatt ccacctggga ccatcgtcag cattcctatc
    27961 aatgtagcaa tcatgaacat aggatagatc gaaagcatcg aaatatctat tctcatcaac
    28021 ttgccgatga tcaaaaatac cgcaagggct cccgtccatt gtccaaagga tgccagtagc
    28081 agaccaagca agccttttgg ataaaactcg aagaacaatg tctttctctt tagataagca
    28141 aaaaagaaaa tgactggtgc taaaaaacta ccgccgaaaa gccagatcca atagcttcga
    28201 aaaatacttt ctgactgaat aaaaaagaca tcaataaatg taagaaacga ccatacagac
    28261 aaaccagtca gcataaatag tgcgactttt gatacagtgg ctaaaaccat ttttctattt
    28321 gtctgctttc cataaaaact agcccgcaga cttgctccta ccacaccacc aaaaccggcg
    28381 agattgttga tcgtgtttgt tacccacgca gcaagaagaa actcttttct ttccattttt
    28441 ggttttcctt gtttttctaa gactctgatc gtcacccaat catacaacaa catcggagtg
    28501 acacccagca gtccagccag aatcatgagc aggatcgtca tgcggctttg ttgtcccatc
    28561 ctttgccaaa tatcttgcca actcatgcca ttagcaatat ttgcgacttg atttgccaca
    28621 aagactaaca cggagccgaa aaaaatcagt tttaaaatca gactatgttc ctttatccat
    28681 tgcagcatca agtatcgttt atttttcaca ttttcccatt cctaacttgt atacttttgt
    28741 gtcatacccc aatgttatct tatttatttt aatttttcca ctttagacct gttctttgct
    28801 tgcgatgata gaaaaagaaa gatatcctaa tgctaaagga gaggataaca tgaaagtagg
    28861 atttatcggc acagggaata tggcacaagc tattattgat ggtttattaa aaaaaggaac
    28921 agtctcgaaa gaagaggtgt tcgtttccag tggacatttt gaaaatgcac aaaaatttgc
    28981 tgctgaaact ggcgtccatg catgcaaaag taatcaggaa gttgcatcac aatcagatat
    29041 cgttctttta gccgtgaaac caaaaatcat tgaaaaagta ttggaagaac taagagaata
    29101 tagccagaaa gtcttattca tttctattgc ttcaggaaaa tcattaatag atttagaaac
    29161 agcgcttgaa gatcctcagg cctccatcgt acgtgtgatg ccgaatgtca atgtcagcgt
    29221 aggagcaggc gtttctgcga tttgcaaaaa tgaagctgcc acggaggaac agcttcagca
    29281 agctacgatg atttttgaag caatcggcag cgtctatcct ttagcagaat cagatttcag
    29341 tacattcatt ggccttgctg gcagctcacc cgcattcgtt tacatgctga tcgatgcgat
    29401 gggacggact ggtgtactga atggtttgtc caaacagaca gccactgaga ttgctgcaaa
    29461 agcaattatg ggttcttgtc aaaaattcct ttcaagtgat aaaaatccat gggaacttgt
    29521 agaccaagtt tcttctcctg gcggtacaac tgttgcaggt gtagttgcct tagaagaagc
    29581 aggattcatc ccagcaatca tcaaaggaat caatacgacg atcgagaaag atcttgagat
    29641 gttgaataaa aaataatgat agcaatgttt tttcgtgcct tacaaaaaaa gaagagctgt
    29701 ggcttttgcc gcggctcttc ttttgtattt tttattattt agcataatca gttgcacgag
    29761 tttcccgaat aacgattact ttgatgtgtc caggataatc cagttcttct tcgattcgtt
    29821 tacgaatatc acgtactaaa cgaacagcgt ctaaatccga gatttcttct ggcttgacca
    29881 ttacccgaac ttcgcgtcca gcttgaactg cgtaacttga ttctacaccg gcgaagctgt
    29941 tcgaaatatt ttccagactt cttaaacgac ggatgtagtt ttctaatgat tcacttcgcg
    30001 caccaggtct tgctgctgag agcgcatctg ctgcagctac aaggacagag ataacagaag
    30061 tagcttccac atctccatga tgagaagcga tcgcgttgat tactactggg ttttctttgt
    30121 actcggcagc taattccgca ccgatctcaa cgtgagatcc ttcaatctca tggtcaagtg
    30181 ctttcccaat atcatggagc aacccggcac gttttgctaa ttggatatct tcgccaagct
    30241 ctgctgctaa gataccagct aattttgcaa cctcgaccga atgcttcaag acgttttgtc
    30301 cataactagt tctgaatcgc agacgtccga tgatcttgat tagatctgga tgaagtgtat
    30361 gagcaccgac ttcaaacgct gcctcttccc cgtattcgcg aatacgttca tccatttctt
    30421 tacgagattt ctcgaccatt tcttcgatac gcgctggatg gattcgtcca tcttgtatca
    30481 gtttttccaa ggtcatccgt gcgatttctc ttcggattgg atcgaatccg gaaagaacaa
    30541 ctgcttccgg tgtatcatcg atgattaaat cgataccagt cagcgtctca agcgttcgaa
    30601 tattacgacc ttctcgtcca atgatacgac ctttcatttc atcattcgga agggtaacga
    30661 ctgaaacagt cgtttctgaa acttgatctg ctgcgcagcg ttgaatcgct aatgataata
    30721 agtttttcgc tttgcgatct gcttcttctt tggcacgctg ttcagattct tttaccatta
    30781 gagttaactc atgattcagc tcttcttcag tcgatttcat gatgacatct cgtgcgtcct
    30841 cttttgataa accagcaatt ttttctagtt ctttttgttg atcttcaatc attttttcaa
    30901 cgtctttttc tcgctcttca attaattgct gttttgaact aagtgcttct tctttacttt
    30961 caagtgagtt ctcgcgtttt tctaacgagt catctttacg attcagcgtt tgttcgcgtt
    31021 gaatcaaacg attctcttga gattttaatt ctaacttact ttctctcagc tcactttcaa
    31081 cttcagaacg gtatttctga ttttcttcct tagcttctaa caatgcttct ttttttagag
    31141 tttctgcttc tttttttgca ctatcgatga ttccagcagc tgatgattgt gcaccagcta
    31201 tttctttctc gtgacgtgac tttgcgacca taaaccctaa accaagaccg acaattaaac
    31261 cgatgatagc gaggagaatg ttgaatacca taaaatccac ctccatacta tctttttcat
    31321 ttttatcatc tatacagctt ctatgataaa ttattctaaa ctcgtcaata tgcatcttcg
    31381 catatatgtg tacaaaaaac atgcacaact ttattctaat gtttgtaccc cgtagtgtca
    31441 actttaaata aacaaagaaa cggctttcta cttattcatt aaacgatggt ttttaaaata
    31501 aatgacacta gtttcatata ggtaaacctt taaattgaga aacttttcta ttaaattatt
    31561 ctttgtagat gaacagatat ttttatttta tgtacccact cgtataatta aataactcac
    31621 gatttcttta ttcaataaaa aaacggcagg tcaaggagag tacctactct tcagaccagc
    31681 cgtttttgtt tatcataatt tttattcttc tactaaaggc aattcttctt gtcctttttc
    31741 atcttctggg acagcgactt cttcaccaat accataagct gctctaacta gagcagaaac
    31801 ttctgccatc atttcaggat gattagccat atagatttta gcgttttccc gtccttgacc
    31861 aatccgatct tctttataag aataccaagc accgctttta tcaacaatgt ctttttctac
    31921 tgccatgtct agaagttctc cttcttgtga gatccctaaa ccatacatga catctacttc
    31981 agcgattttg aaaggaggtg ctactttgtt ttttacgact ttgatctttg tacggttacc
    32041 aacaatatct gtaccttgtt tcaattgttc tgcacgacgt acttccaaac ggatcgttgc
    32101 gtagaatttc aatgcgcgtc ctccaggagt gatttctgga ttcccaaaca tcacgcctac
    32161 tttttcacgg atttggttga tgaaaatagc gattgttttt gttttgttga tcgaaccaga
    32221 gagtttacgc aatgcttgag acatcaaacg tgcttgtaac ccgacatgtg agtcacccat
    32281 ttcgccgtcg atttctgctc gtggaactaa agcagcaact gaatcgacta ctacgatatc
    32341 tacagcccca cttgatacta aagcatcagc gatctctaga ccttgttctc ctgtgtcagg
    32401 ctgtgaaaga agtagttcat cgatattcac acctaatttt tgtgcatatt gcggatctaa
    32461 cgcatgctca gcatcaatga aagcggccgt tccgccattt ttttgtactt ctgcaatagc
    32521 gtgtagtgca actgttgttt tacctgaact ttcaggtcca tatacttcga tgatacgtcc
    32581 acgggggtaa ccgccgacac ctaaagcgac atctaacgca agagagccac ttggaattgt
    32641 agagatttgt tggtcgattt tttcgcccaa tttcataata gagcctttac catagctctt
    32701 ttcaattttt ttcagtgcag catctaaggc tgctttacga tcatctgcca atacgataat
    32761 cctccttata tttacaaatc agagcaacat ttcattatcc ttatcatatc ttttttagtg
    32821 aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat cattttctta aaagctgttt
    32881 tctcaaaaga tcaaatcctt gcataaccgc attatatctg atagcagcac gatctctggc
    32941 aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa atagcgatcc atactgttcc
    33001 tgcagattga ccttctaacg tgtccggtcc agccactccg gtgaaagcta cactgtaatc
    33061 tgttcttgct atctcacgtg cttgaatggc catctgctcc acacattctt tgctgactgt
    33121 tccatattct gctaatagct ccggatcaat ctccagaaaa ccagcttttg tttggagcga
    33181 gtaggtaacg aatccgcctg gaaatacttc agatacacca gcaatgtcgc ctaatgcggc
    33241 ttggaaggcg ccggctgtca aactttccgc tgctgttaca gtctgcttgt tttctttcag
    33301 tagttccact acgacttttg ctagcgaatt atcatcgccg tatccataaa aatattcccc
    33361 tactcgttct tggatttttt cttctaatgc taaaagagct tgattccctg cctgaacatc
    33421 atttgttttc actgtcagtc gtaatgtcac ttcatttggt ttggcatatg gtgcgatcgt
    33481 tggattgatc tgtgtctcaa tcagatcttt tagctcagtg accagtcgcg attcaccaat
    33541 cccataaaag cgcaaaactt tagaaattag tttttcttca gaaggaaaac gttcctctag
    33601 taaaggacgt acttgttcca caaacattgg tttcaactca ttaggcggac cgggaagaag
    33661 gatatacgca tgtgtatctg tttgataaaa agtccctaaa gcaagccctg tacgatttgg
    33721 tagaggaacc ccgccttcga taatctggga ctgttgcaga ttgtttttgg tcatttttcg
    33781 atgggttgtt tcaaaatatg ccaataattt tttgtatcct tccgtatttt gtatgagaga
    33841 cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca tcttctgtcg gtcctaatcc
    33901 gccacataag agtatgagtt cacttcttgt ttcagctaga gcaatcaact cttctaaacg
    33961 ctgctgatta tctcctacaa ctgtttgata ataaacttcg atacctagat cagctagctg
    34021 ttctgacaag aaagtggcat tcgtattcac gacttgtcct agtaaaagtt ctgttccgac
    34081 agctattatt tctgctttca ttacttcccg ctcccttata tagtatacgc tgtattttta
    34141 tattttttta tttaaaaaga gggcaaggag ctgtgacaaa aatcttgtca cagctccttg
    34201 ttccaaataa acagtgttct cctgccaaaa cctcggtcat aagtcacaat atacataaaa
    34261 catgaaaagc tgttttttga aattatccct tgtgactcag agttagacgg caggctcaca
    34321 acctctccac taaacgatac ttacattgat cctttgaaca caccgctgtt tttgatgaag
    34381 taatccacac ctgaatagat agtaaagatc agacaagcat aaagcatgat ttgatctact
    34441 ggaataccga tcaggctgaa tgggatatta ttgataaaca gcaagataat agcaaccatt
    34501 tgtgtagccg tcttgacttt tcccggccaa gctgcagcca tgacttctcc atgttcgaca
    34561 agtagtaaac gcaagcctgt gacggctaat tcacgacaga caatgatcgc tactacccaa
    34621 ccaggggcct ttccttggtc aaccaacatg ataaaagcag tcataactag cattttatct
    34681 gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc ctcgcgcacg ggcaattttt
    34741 ccatctaacc agtctgtgat actagctaaa gcaaaaataa ctgcacctac tagctgtgtg
    34801 acagccagtg tggtgtctcc tacgttaaat tgtccccaat ctaaaggtaa acctacaacc
    34861 aaaatgaaaa tcgggatcat acaaattcta agtacagtta atttatttgg taaattcaca
    34921 ctatcgactc ctttcttccc tattctacgt gactatcagc gaagtttcca ccgatattat
    34981 ttacttttcc aaatgaattt attcaaagga gtatttttat tgaaattgat ctttttgaat
    35041 taacgaagaa ggaccgcgac acagatttgt cacgatcctt cttttttata cctacaagta
    35101 aacagatgcg taaactaatt tatacgatca ttctgcgtat tgaagagtaa ggttcacatt
    35161 ttttggactc tgattgtttt cccctggatt aactggtacg gattctccat tcgctttgat
    35221 cgttgcattg cttgctgcac caatcgtgat ggtcgcattt gttgctgttt ccggtaacac
    35281 ggtactctgt gtttcatttg ctgccagtgt cccttgataa acataggcat tgtctactag
    35341 cacacctaac catacacgat tgacagcagt aaattcaagg gttacaggct tggtagcgtc
    35401 tgtgatattt actgcgatgg cactgccaga atcttctcct gtagctattg ccattttctt
    35461 ttcttcactt gattttgttg ttgattctac tgtagaagac tctttggtct gcgtgctttg
    35521 ctctgaggaa gaggactcct cgactgatcc ttctacttta agcgaagaag ctgtgtctcc
    35581 gattattgga tcagcttgac gatcttgcca agtcatatag cccacgacaa cgacaatcgc
    35641 caaagcaacc aatcctaata aaatgacagg aagacttgtc caaaagcgac ttggatgcga
    35701 ttcttcctga tgcattgctt ttctagaagt acttactgtt tcaggctgcg gacgttcagg
    35761 gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg actaaatggt ctccatcttc
    35821 tcctacagct tcagcgtatt gccgaataaa tgcccgcacg taaaatttac caggcaattg
    35881 gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt tgaatttttg taatctgctg
    35941 taattcatct aaagatattt ttttattcag gcgtgcttgc cgtaattttt ctccgatggt
    36001 ttcaaaagcc actcgttcat cttctccagt ctgtttgatt ctccgaagca tctgctgttg
    36061 caacgaaaga cgttatgttt gggtgctgtg acaaaagtca tgtcacagct ccttttttcc
    36121 gaataaacat tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aataaatata
    36181 agaagctgtt ttttgaactt gttccttgtg actcggagtt aaacggcagg ctcacgacct
    36241 ctttcttaaa cggtgttcaa tcagctgttc ctcggtatta gctcaaaaac cggcaaaata
    36301 taaggagata tttttccgta tgtttctcta atactcaggt caaaacgctg atttcacaac
    36361 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa
    36421 aacattaaga agctgttttt acttggctgt tcttattact tgatgcaggg cagctttttc
    36481 acaacctctt ggtacacggt gttccagaag tagcttcttc ggcaataagc cgaaatatca
    36541 caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct
    36601 gtcacaacct ctttgattac ttcgtttgat tttattttaa tataagttga ttaaaagaga
    36661 gcatgatttg ttttattttc tcactaattt ttccaacaca actaagaata ccataaaagc
    36721 tgattttgga ggaggttcct actacaagtc taaaacgaaa tttaagactt ttcctcataa
    36781 aatactggtt tattttggtc tcatgtaaaa ctcgctgaag gcctcttcac ttataaatgc
    36841 ttccccagct gccagtacgt ctttcatttg gatcgaatcg atgatttcag gaagatcaaa
    36901 taaagttcga tcgccaaata aactttgcgt aaattgattg gcaatatatt cgatcgaatt
    36961 taatgattgg aaatattgtc caagcatttt cttcttcagc aaatcgagat ttgtttctga
    37021 cacttccgga tcatcagcga atccaagaat gatttctttg actttttctg ctgctttctc
    37081 tggttcgtcc gtatctccgc tgaagtccgc aaagtgaaat tcacgatcca agctgaattc
    37141 aaaaccaaag ctgtcatcga taattccttg attgtacatc gctagatagt ttcgagaagt
    37201 attgcctaaa atcatttgga acaacagatt gattgctgtt ttatagcgta acagttctgt
    37261 tccttcttgc ggcaatgtgt ctaatccttt aataccaagt acaaatttat ctcgtgtaat
    37321 ggctgcttcc aatgcacttt gtttgatgat ttcttttgtg ttttcaggga aataacgtac
    37381 aatttcttgc tttggaggga aattttttgc ttcttggttt tcccgaataa gtttcatcaa
    37441 tttctcaggc tccatcttac caactacaaa taaaaccata ttgcttggct gataaaacgt
    37501 ccgatagcac gtatacaagt cttgagctgt gattttgtcg atactttcaa ccgtgcccgc
    37561 aatatcgata tgcaatgggt gtgtaggata caagttattt agtatgccaa aaaacatccg
    37621 ccagttaggg tcgtcttcat acatttggat ttcctgaccg atgattcctt tttccttgtt
    37681 tacagtttct tctgtgaagt aaggtgcttg tacaaaatcg atcagagtcg tcagattctt
    37741 ttctacctga tccgtcgtag aaaacagata gctggttttc gtaaaacttg tgaaagcatt
    37801 ggcggaagct ccttgtttcc caaataattg aaagacatca ccatcttctt tttcaaacaa
    37861 cttatgttct aaaaaatggg caattccatc tggaactttc accttttctt tttcaccata
    37921 gggaataaat tcgttatcga tcgaaccata attcgtactg aacaaaccat atgtcttgtg
    37981 atagtcattt ttaggcaaca aatacacggt caaaccgttt ggcagtactt catgataaag
    38041 tgtttcattg atttgctcat attctgtttt attcatttgc atttcctcct gctaaaaaga
    38101 agattgcctg caattcgatt tgctcagcta cttgttgtat ctctttgatc gttacgctct
    38161 ccatacgttt caaccattct tcatcagata attttgtttc cggcagccag ctgtccaaaa
    38221 aagaagcttc gattgccgct tgcggagagt ctagagaaag cagatattgg tttctcagca
    38281 ttgcttttgt ctgagacagt tccaactctg ttatttcacc atttcgcaaa ctttctaatt
    38341 gctcgtgaat cagatgaagt acttgatttc ggttcttttc atcgataccg gtttggacac
    38401 tcatgaatcc tcggaacgta tccacgctac tagaggcata ataagctaga ctttctttct
    38461 ctctgacatt catgaaaagt ttagagtgag gaaaaccgcc aaataaaccg ttaaagacca
    38521 ttagcgcaaa acgttctggt tcatcgtaat acacatttgt ctgataagct aggttcagct
    38581 ttgcttgaac gattggctca cgtacttgtc gttccttcac gatattcact tgaggttggg
    38641 tataaaacag atctggataa actgtttccc gcgactcaaa tggtaattgc ccaactgctt
    38701 cagctgcttt gtccggatca acatcgccga caacaaaaat gtcgatttga tcatttgcca
    38761 tcatttcttg atagtaggac gctagcgaac tagcagtgat cttgtccaaa tcttcgacta
    38821 cgcccaaccc tgggatcttt tgttccgggc tgttttcaaa ataaagttct tgcaaggcat
    38881 aggaagcaaa agtttgttta tcttccttca aactttccaa atatagacgc agattgttct
    38941 tttccaagtc aaacgtcaac tgatcaaact gatgattttt aatatttgga taaaataaaa
    39001 cctcttttaa aaaatctacc gcttgagaaa aaaggtttgg atcattgaca tattcaccat
    39061 tcacaaaact gatccctgcg tttagccaat gtaaatttcc tttttttcta actgataatc
    39121 caaagcttgc gccataaagc tctgcaagtt gcgcacttaa ctttgtctga tccgaataat
    39181 tcaaactatt tgtttccaac atgctgctga gaagtgaacg tttcgtgatg acttcttgtt
    39241 gcagacgcgc agtaaaacgg ataaaaatcc ggatcgtttt gtattgtttt gttggtaaga
    39301 catgaagatt cacgccttct gccaattgat acaccatatt tatatgctcc ttttcacagt
    39361 tgattgcccg cttactcttt taatgggtca acctgccttt tttacttatt cctctgcttg
    39421 aaatttataa gttcaggcaa ttcgccgttt tttctaaatg atgttacact ttatagtata
    39481 gcatagatgt tttttatcaa aagaaacaga cggctaaaag cgtatattgt ttttttattt
    39541 tttatttttc gatatactaa agacaaataa aagaagggag ggtcattcat gatcaaagaa
    39601 ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt tgttattggt
    39661 agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc actaataggt
    39721 tggtttgtcg caatgatcac aggtacttct gatttagaag gagcactttc aattcttgac
    39781 tggtcgccga caaaaggcgt cacatttgcg tttggtaacg ttattagtgc tatcattaca
    39841 tttctgatca ctggttttgt cttattctta gttgtcaaag cggcaaaccg tgcgaagaat
    39901 ttacgctcca aagaagagca agtggaagtg gaagaaactc ctactgcaga agattatctt
    39961 agtgacatac gcgatcttct tgccaaacaa gtggcagaag acaatacaca tccagtagag
    40021 caaacaaatc tttccgatac ttctaaataa taaagaatct aaaaaaaaga aagccgattt
    40081 tcagtgtcgg ctttcttttt ttagattctt aacttgctac ttgttccttt ttcatgtcga
    40141 tatgtggaat atcatcttcc aaatacacat ctgagattgc cttgaatcca aacgactcat
    40201 aaaaagcttg taggtattct tgtgcgccga tcacgattgg tttttctgga aaatttttct
    40261 ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa accatcttta cggttgtcct
    40321 gatgaacaag gacacgtcca aaatggatac tctccgcttc ttcatatact cgcgtgtagg
    40381 caaggatctt cccatcagta tcttgaaaaa acgtatgcca agcttctcca tcaatgtcat
    40441 caacttcttg ataaggacac tgttgttcca caacaaaaac tgcgattcgt tctttgacga
    40501 tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt tacctgatat tccactaagc
    40561 aaacgccccc tgctttttta ttcttagcat accgattcgc acacaaaaaa gcaaacgaac
    40621 cttacgaatc tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac
    40681 tgtccgagta agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg
    40741 atccaaatca acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt
    40801 ttgacgcttt cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa
    40861 atctttcttg attgaagcta aagttgttgg catacagttc acctctacta tcaactatac
    40921 cacatatttt taaaattttc aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa
    40981 taagaagctc attaagattt ttctaaatta ttcctcatta taaaatttca agtgtttttt
    41041 tattttgagt tttactctcc cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca
    41101 ttcagccatg tctacaaaat caacgccttg catataataa tgaaaaattt tagcttctat
    41161 ttttgaaaac tggatttcgc aattttcgat tgcttctttt aataaaagat gctgaattgc
    41221 taaaggttct tgcaatgtga tctctgctaa aaagtctggt ccttctgtct ctattttttg
    41281 gtcgattgag acttcatctt tttgatcacg acgcttcagc gcgttctgct tcctcaacag
    41341 agaatagatt tgattgctta taacgctttt ataataactt ccaaagctaa cacctttgct
    41401 tttttcgtaa gttctcaatg cctgatataa agcgatcctt gcttcttgtt cccaatcctc
    41461 agaaacataa tttcttaaat aataggtgtt tttcaaacat agtaaaagtg gaaaatattt
    41521 ttgatacagg gcagcaaatg cttcttcgtc accattacgt gcagcgcgca ataactcctc
    41581 agacataaaa taattcctcc ataaattcgt ttatggagga atcttatcat caatgattca
    41641 tcgaactgat tagaacgata tgttatttat tttttctcat taaatcgtcc aatttttggg
    41701 acaatttttt taactgctct tcattccatg gagaattacg tcggaaatca ataaaatgca
    41761 tatctttatt ttcttgggct attgtttttt ccgttttttt tatcgtctta tataattcat
    41821 aagcagaagt tcgtaatgct ccttgggaaa agatcatcca ctgttctgct aaatcgcttg
    41881 tcgccactgt cacttgtgtc aaacggtcat tcagctgacc agcgattctc tcgatataac
    41941 tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa ctggtatttc ttataatttt
    42001 gttggatgcc cggaactagt tgggcatcga agacaacgat gacttccatt ccttcatatt
    42061 tcgcataatt tgataaacga tgtaaaagag cttctcttgc atccgccatt ttgtcctgct
    42121 ttttaagctg gacaagttcc ggccacgaac caatcatgtt gtagccgtct acaatcaaca
    42181 gctgtttttt catggtctac tccccctgac gttgggaaaa agcctgatac ataagcaaac
    42241 cagcagcaac actggcattt aagctctgga cgtgcccggt catcgggatc gtcagcaatt
    42301 catccatttc ttttttcaat cctggactca ttcctttgcc ttcattacca atgactaaag
    42361 caattgatcc tttggcattc catttttgga agtttgtgcc ggtcatatct gtgccaaata
    42421 cccaaaatcc tgtttctttg agctgttgca cagcttgtac caaattcgtc acgcgtgcaa
    42481 cagggacatg ttcgactgcc ccagtggaag tctttgttac gataggtgtg attccaactg
    42541 ctcggtgttt tggtatgatc acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg
    42601 acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt
    42661 tctcttttgt tcgttcaatc agttggtcaa gggataaata ttcataaggt gtaatcgcaa
    42721 gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc
    42781 atttgactgg cacagcttgt tcacgtgcga tttctttcag ttcttctact ttttctcctc
    42841 gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg ttgtagcgct tctactgcgg
    42901 catggtgccc aaagacgaaa ttatcttcta atgcttcttc attttgatca gaaactgact
    42961 gatttcgtcc agtttgcttt ttatttgaat atctattttg ttttttgtct tcaaatccac
    43021 gtttcgattg atgtttgttt gttctttctt tacccatttt tttcaccaac tttctgaata
    43081 caccagcgga tcaattcttc catccgctcg gtttgttttg tcaaatgcag atagcccatg
    43141 agcgcttcga accctgttgc gacccgatac gtcgtaacat cagcattttt tgcgctcgta
    43201 tggcttttag catttcttcc tctgcgataa taaagttctt cttcttctgt caaaagatgt
    43261 tcttccagca tttgctgaat caaaaaggct tgtgcattcg cagaaacata attcgttgcc
    43321 gcacgatgca gcttattggg ttttgtttct ccttgatcaa tcaggtggtc acgaacatag
    43381 atttcgtaaa tcgcatctcc aacataagcc aaggctaaac cgttcaattg tgtgtaatct
    43441 ctcatgcttc tcttctccat cgggttcctt gcggtgtatc ttctaataag atcccttttt
    43501 ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat
    43561 ttcgttcttc gatcaattgt tcaatctctt catctaataa ctcttctgct gtaaataaaa
    43621 taccgaagat cgaaagccat tgcgagaata aagcttctcc ttgtgccaag acagcggcag
    43681 aaactttcgg ctggtctaga taagtgttca gccacttagc aagctcatag accactgtga
    43741 tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt
    43801 ctaacaaagc gattttatcc gcatcatctg ccaaattctc tgaggcagtt tcttggcgga
    43861 atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg
    43921 tcgtttcact gtagcggatc ggacgacggt attgtgtggt cgccatgaag aaacgcaaga
    43981 cttgtggatc aacgtttttg atcatttcat gtacagtgat gaaattccct agagatttac
    44041 tcattttttc atcgtcttcg ccaatcgtta catagccatt gtgcatccag taattagcga
    44101 attttttccc tgttttcgct tcactttgtg cgatttcatt ttcatgatga ggaaattcta
    44161 aatcttgccc gccaccatgg atatcgatcg tttctcctaa atgttttgtt gccataacgg
    44221 aacattcaat atgccaaccg ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc
    44281 caggttttgc ttgtttccat aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt
    44341 cccctgtacg ctgacttgct ccgacttcca gttcatcgat ggattggtca cttaatttcc
    44401 cataattttc aaatttacgg gtacggtaat acacatcacc tgaagattca taagcatagc
    44461 ctttttcaac caacacttga ataaatgcta aaatatctgg catatgatcc atgactcttg
    44521 gatgcagtgt agcaggttcg acattcaacg ctttcgtatc ttcttcaaat gctcggatga
    44581 aacgatcagc aacttctggt gctgtaatac ctaattcgtt tgctgtacgg atgattttat
    44641 cgtctacatc cgtgaaattc gatacgaaat cgactttata gcctcgatat tcaaaatagc
    44701 gtcgaattgt atcgaaagca atggtgctac gagcatttcc gatatggata taattataca
    44761 cagtcggtcc gcagacatac ataccgacat gtcccttttg tatagggatg aattcttctt
    44821 tttttctggt caatgtattg tagattttta tcattctgat ctcctcactt tttctccttt
    44881 gattcgaacg acttttgctg gtactcctac cgctgtagcg tcgtctggaa catctttcaa
    44941 cacaacggca cctgcaccga ttttggcgtt ttttccgatc gttaccggtc ctaatatctg
    45001 cgcattcgca gagagcatgg ctccttgttt aacagttggg tgtcttttac ccgtttcttt
    45061 tcctgtgccg cctaatgtta cgccatggaa taaaatcaca tcgtcctcga tttctgcggt
    45121 ttcgccaatg acgattccca ttccatggtc gatgaacacg ccagttccaa tcgttgctcc
    45181 tggatggatc tcaacaccag ttaggaaacg ccagaattga gcattgatct tcgccaataa
    45241 aaagagccgg tgctgatata aaaaatgaga gaaacgatgc caaaacaagg catgaaaacc
    45301 agggtatgtc aaaacgatct cagctgtcga acgtgcagcc ggatcgtttc tctttgctgc
    45361 atcaatcgct cgggtaaacc aactcatgac tttccactcc tttcgtaaga attttatgta
    45421 ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa taagtttcct tatcaaaaga
    45481 cgctttccgc gtggttccac ttttcttcat aatcaatgat tatctctgac agctaacgcc
    45541 tgttatacgt tcttggctac tttgtttcct ataaaacagt ttttcctgtt ttctgtgttt
    45601 cgccaagacc actcccagag gcatttcggc aaccattcat tgataccttg caccacccgg
    45661 tatctctctt gtaatgatca gctgcttact tttttctgtt catcgtgttt agttcagtac
    45721 ttgatttaaa tgtgctaatg atttttcacg accaagcaat tcgatcgttt caggcaattc
    45781 tggaccatgc atttgtcctg atacagctac acggattggc atgaaaagat tttttccttt
    45841 tacgccagtt tctttttgaa cagctttgat ggcagctttg acagttggag catctactac
    45901 ttccatttgt tctagctgtg ctttgaatgc gtggagaaca gtaggaactg tttcaccagc
    45961 aagtacttct tttgctgctt cattcaatac aggatgttca ttgaaaaata gcgtagacag
    46021 atcaacgatt tctgcagcat agctcatttg aggttgatac aggctaacga tcttcttcac
    46081 ccattcgatt gtcttttcat ctgggttttc tgctacacgt ccatcagcga ttagataagg
    46141 cagacacatg tcagtaaatg catctagatc cattgctttg atgtaatggt tgtttaccca
    46201 ttgcagtttt ttcgcatcga aagctgcggg tgatttactt aggcgttttg gatcgaacat
    46261 tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc acaggtgacc agcccaataa
    46321 tgcaataaag ttgaacattg catctggtag atagcctaat tcgcgatatt gttcgataaa
    46381 ttgcaagatt gattcgtcac gtttgcttag ttttttacct gtttcactgt tgatgatcaa
    46441 tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct tcatagatca tcagttgttt
    46501 tggtgtattg gcaatatggt catctccacg taaaacgtga gtgattttca tcaaatgatc
    46561 atcgaccgca acagcaaaat tataagttgg cataccatcg cgtttttgga tgacaaagtc
    46621 gccaccgata ttatcggatt cgaacgaaat agcgcctttc accatatctg taaatgcgta
    46681 ttctgtattg cgaggcacac ggaaacgtac aacagattct agaccttgtg cttctttttc
    46741 tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg taatgaggca tttctcctct
    46801 tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct gtgcaatagc atttgtatgc
    46861 tcgattgcta gcaagcagct gatcaatcag cggttgatag atttctttac gttcagattg
    46921 acggtaaggg ccgtattctc caggattttc aggagattca tcccaattca ttcccaacca
    46981 agcaaggttt tccaactggc ttttttcgcc atcttcgata ttacgttttt ggtcagtatc
    47041 ctcaatacga ataataaagt ctccatcatt gtggcgggca aacaaataat tgaataatgc
    47101 agttcttgca tttccaatat gcaggtgccc agttggactt ggtgcatagc gtacgcgtac
    47161 tttcgtcatt ttcttcctct tctttcaaac tagactttga ctaacagcaa aaattccgtc
    47221 agttacggct tctcaaatat catttattta aagccactca gcacaaattt taccttttct
    47281 aaggtaaata gtaaagccaa ctggttgaat ttccttacca gttggctgaa tttctttttt
    47341 attttgattg tttagtcttt ttctctgtct cttttttatt gtcgattcct cgtcctgcat
    47401 gtgcaggttt agcaaagatc atgcgtccag cagctgtttg aagggcactt gttacaacga
    47461 cttcgatacg ttcattcatg taatgctgtc catcttctac gacaaccatc gtgccgtcat
    47521 caaggtaggc tactccttgt tggcgttctg tcccggcttt tactactaga acattcatcg
    47581 tttcaccagg gatgacgact ggttttacgg catttgccaa cgcattgata ttaagaacag
    47641 gtacattttg gaattcagag actttattca agttatagtc attggttaca atgacaccat
    47701 ctaaaagctt agccaatttg atcaatttgc tgtctacttc actgatatct tcaaagtctc
    47761 catcgtacat ttcgacagat ataccctctt ctttttgcaa ggcatttagg atatctaatc
    47821 cgcgacgacc acgcacacgt ttcaaactat cccctgagtc cgcgatgtat tgtagttcgt
    47881 aaagaacaaa attagggatc atcaaggttc cttctaagaa tccagtcttt gtaatatcat
    47941 aaatccgacc gtcaatgatc acgcttgtat ccaagatttt atatttatgg aaattttctc
    48001 ctgattttcg ttctaacagt tgactctcaa cttgactagc tgtttcttgc tcgatttttt
    48061 tgcttcgtga accaaaaact ttcttccatt cttcgattcg tgtcgttccc attctcaatc
    48121 ccagatagcc taagatgatc atcagtaaaa tcggcaatac actattgacg aatggaattt
    48181 ccatattgta aagaggggtc gagatcacaa cacctaaagt caatccgatg atcgcaccga
    48241 tcgcaccgaa gagaagataa gtcaagctga cctcacttag tttttgttct acttgtttga
    48301 taatagctga gatatgtttg gccaacagca aagataataa aaagaaaata agtgcgccta
    48361 acaggctgtt ggtaaatgta ttattcagcc atccgtttga ctgttgaccg attgcctgcc
    48421 aagctgaggg taaaaatgaa atacccaagc ttgcgccgac aatgatcatc agcaaagtaa
    48481 tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc atttattagt tagcggaaca
    48541 cacgcttcaa cgtttcagcc aaagttgcta ctccaactat ctcgattcct tttggcgctt
    48601 cccagttacc taagttgttt ttaggtaggt atacttttgt gaagccgagt ttttggactt
    48661 ctttgactcg ttgttcaata tgactgacac gtctgatttc tccagtcagt ccaatttcac
    48721 cgatgaaaca ttccgttggc tgtgttcctt tttctttata gcttgaagca atactgacag
    48781 cgatagctaa atcaatcgct ggttcattta attttacccc acctgcagct tttaagtaag
    48841 catcttgatt ctgaagaagc aacccagctc ttttttccag cactgccatg atcagagata
    48901 cccgattgaa atcaagacct gttgttgttc gtttggcatt tccgaacata gtaggtgtga
    48961 ctaatgcttg gatctctacc aaaattggac gtgtcccttc cattgccaca acgatagcag
    49021 agccagttgc accgtctagg cgttcttcta agaaaatctg tgaaggatta gcgacttctt
    49081 ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt cgttgagcca aatcggtttt
    49141 ttactgctcg caaaatccga aaactatgat gttgttcacc ttcaaaatat aacaccgtat
    49201 ccaccatatg ttctaacatt cttgggccgg cgatcgagcc ttctttggta acatgaccga
    49261 cgataaaaat ggctatccca tttgttttag cggttttgag tagttctgca gtggtttctc
    49321 ttacttggct gacacttcct gccacacttg tgatgtctgg ctgcgtcatc gtttggatgg
    49381 aatcaatgat aacataatca ggggagatat gttcgatggc acgactgatt tcgttcatat
    49441 ctgtttcagc gtatagataa aattctgtgt tgatacttcc gaggcgttcc gcgcgtaatt
    49501 ttatctgctc tgcgctttct tcgccggata catataagac ttttccgcct atggctgcta
    49561 attgctgaga aacttgtaac agcaaagtgg atttcccgat acctggatcg ccaccaatca
    49621 agaccataga ccctggcacc acacctccgc ctaacacacg gttcagttct tctaattttg
    49681 ttttgatccg aggttctttt ttagggacga cgtctgcgat ttttgttggt tttgcttttt
    49741 ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc ttgttcgatt tcttcgacca
    49801 tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa cttaggcgac acatacccgc
    49861 aattttggca aacaaattgt acttttgatt tttttgccat tgtaccgcct ccttttatcc
    49921 taatcatgag acattaaatc aagcttaagt atctttaacg ttttatttac tggaagaacc
    49981 aaatccgcct tgacgttgag cagtcgtttg atcttgatct gcttttaaaa acggcaaaaa
    50041 gatcccttgg ccgatacgct cgcctttttt gatcactaca tccttgaaac caaagttggt
    50101 gaattggaac atgatatggc cttcatttgc ctcattattg taataatcac tgtcaatcac
    50161 acctactccg tttgtcaaca ctaaaaaccg cttcaacgga ttgcttgaac ggttagcaag
    50221 ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca gtaggcacga gggttggttt
    50281 caattgtttt tcattttctg atacatcttt tttcaatcca tctgcgactg tttccaaccc
    50341 ctttttccag cctgctttcc agatacttgg aatcacgacc tcttctgctg cttcaaaatc
    50401 atagccagca gcatgatgtg tagcgcgctg cggtagattg atattttgat ctttatattt
    50461 agaaataact tcaaatcctc tttttctcac cggttttcct
//
LOCUS       DAZULG010000008        50130 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000008 DAZULG010000000
VERSION     DAZULG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.008"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..1620
                     /locus_tag="ACG9CY_000385"
     CDS             61..1620
                     /locus_tag="ACG9CY_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4936926.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            1617..2981
                     /locus_tag="ACG9CY_000386"
     CDS             1617..2981
                     /locus_tag="ACG9CY_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4936927.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            3152..4222
                     /locus_tag="ACG9CY_000387"
     CDS             3152..4222
                     /locus_tag="ACG9CY_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4936928.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            4237..4905
                     /locus_tag="ACG9CY_000388"
     CDS             4237..4905
                     /locus_tag="ACG9CY_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4936929.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            4925..5503
                     /locus_tag="ACG9CY_000389"
     CDS             4925..5503
                     /locus_tag="ACG9CY_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4936930.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            5904..6815
                     /locus_tag="ACG9CY_000390"
     CDS             5904..6815
                     /locus_tag="ACG9CY_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4936931.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(6903..7820)
                     /gene="rnhC"
                     /locus_tag="ACG9CY_000391"
     CDS             complement(6903..7820)
                     /gene="rnhC"
                     /locus_tag="ACG9CY_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS4936932.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            8022..8459
                     /gene="zapA"
                     /locus_tag="ACG9CY_000392"
     CDS             8022..8459
                     /gene="zapA"
                     /locus_tag="ACG9CY_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS4936933.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8518..9066
                     /locus_tag="ACG9CY_000393"
     CDS             8518..9066
                     /locus_tag="ACG9CY_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFS4936934.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            9181..11541
                     /locus_tag="ACG9CY_000394"
     CDS             9181..11541
                     /locus_tag="ACG9CY_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS4936935.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            11628..11942
                     /gene="trxA"
                     /locus_tag="ACG9CY_000395"
     CDS             11628..11942
                     /gene="trxA"
                     /locus_tag="ACG9CY_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS4936936.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            12124..13902
                     /gene="uvrC"
                     /locus_tag="ACG9CY_000396"
     CDS             12124..13902
                     /gene="uvrC"
                     /locus_tag="ACG9CY_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS4936937.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            14047..14946
                     /locus_tag="ACG9CY_000397"
     CDS             14047..14946
                     /locus_tag="ACG9CY_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4936938.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            14939..16201
                     /locus_tag="ACG9CY_000398"
     CDS             14939..16201
                     /locus_tag="ACG9CY_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4936939.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            16362..18260
                     /locus_tag="ACG9CY_000399"
     CDS             16362..18260
                     /locus_tag="ACG9CY_000399"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS4936940.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(18590..19309)
                     /locus_tag="ACG9CY_000400"
     CDS             complement(18590..19309)
                     /locus_tag="ACG9CY_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS4936941.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    19499..19546
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            19646..19978
                     /locus_tag="ACG9CY_000401"
     CDS             19646..19978
                     /locus_tag="ACG9CY_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936942.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            19965..21968
                     /locus_tag="ACG9CY_000402"
     CDS             19965..21968
                     /locus_tag="ACG9CY_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS4936943.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            21987..22457
                     /locus_tag="ACG9CY_000403"
     CDS             21987..22457
                     /locus_tag="ACG9CY_000403"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS4936944.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            22478..23062
                     /locus_tag="ACG9CY_000404"
     CDS             22478..23062
                     /locus_tag="ACG9CY_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFS4936945.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            23077..24063
                     /locus_tag="ACG9CY_000405"
     CDS             23077..24063
                     /locus_tag="ACG9CY_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS4936946.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            24053..24364
                     /locus_tag="ACG9CY_000406"
     CDS             24053..24364
                     /locus_tag="ACG9CY_000406"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS4936947.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            24386..26167
                     /locus_tag="ACG9CY_000407"
     CDS             24386..26167
                     /locus_tag="ACG9CY_000407"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS4936948.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            26160..27536
                     /locus_tag="ACG9CY_000408"
     CDS             26160..27536
                     /locus_tag="ACG9CY_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS4936949.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            27543..28178
                     /locus_tag="ACG9CY_000409"
     CDS             27543..28178
                     /locus_tag="ACG9CY_000409"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS4936950.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            28208..29563
                     /locus_tag="ACG9CY_000410"
     CDS             28208..29563
                     /locus_tag="ACG9CY_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4936951.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(29755..31149)
                     /locus_tag="ACG9CY_000411"
     CDS             complement(29755..31149)
                     /locus_tag="ACG9CY_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4936952.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            31241..32212
                     /locus_tag="ACG9CY_000412"
     CDS             31241..32212
                     /locus_tag="ACG9CY_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS4936953.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            32209..32991
                     /locus_tag="ACG9CY_000413"
     CDS             32209..32991
                     /locus_tag="ACG9CY_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS4936954.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(33160..33531)
                     /locus_tag="ACG9CY_000414"
     CDS             complement(33160..33531)
                     /locus_tag="ACG9CY_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4936955.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            33665..34108
                     /locus_tag="ACG9CY_000415"
     CDS             33665..34108
                     /locus_tag="ACG9CY_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4936956.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(34151..34960)
                     /gene="yidA"
                     /locus_tag="ACG9CY_000416"
     CDS             complement(34151..34960)
                     /gene="yidA"
                     /locus_tag="ACG9CY_000416"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS4936957.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(34979..36346)
                     /locus_tag="ACG9CY_000417"
     CDS             complement(34979..36346)
                     /locus_tag="ACG9CY_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4936958.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(36330..37160)
                     /locus_tag="ACG9CY_000418"
     CDS             complement(36330..37160)
                     /locus_tag="ACG9CY_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS4936959.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            37323..37760
                     /locus_tag="ACG9CY_000419"
     CDS             37323..37760
                     /locus_tag="ACG9CY_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS4936960.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            37836..38456
                     /gene="rpoE"
                     /locus_tag="ACG9CY_000420"
     CDS             37836..38456
                     /gene="rpoE"
                     /locus_tag="ACG9CY_000420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS4936961.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            38645..39151
                     /locus_tag="ACG9CY_000421"
     CDS             38645..39151
                     /locus_tag="ACG9CY_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4936962.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            39168..40067
                     /locus_tag="ACG9CY_000422"
     CDS             39168..40067
                     /locus_tag="ACG9CY_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS4936963.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            40154..41038
                     /locus_tag="ACG9CY_000423"
     CDS             40154..41038
                     /locus_tag="ACG9CY_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4936964.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(41230..41301)
                     /locus_tag="ACG9CY_000424"
     tRNA            complement(41230..41301)
                     /locus_tag="ACG9CY_000424"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41266..41268),aa:Arg,seq:ccg)
     gene            41361..42695
                     /gene="rpoN"
                     /locus_tag="ACG9CY_000425"
     CDS             41361..42695
                     /gene="rpoN"
                     /locus_tag="ACG9CY_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS4936965.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            42844..43935
                     /locus_tag="ACG9CY_000426"
     CDS             42844..43935
                     /locus_tag="ACG9CY_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS4936966.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            43976..44977
                     /gene="gap"
                     /locus_tag="ACG9CY_000427"
     CDS             43976..44977
                     /gene="gap"
                     /locus_tag="ACG9CY_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4936967.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            45087..46277
                     /gene="pgk"
                     /locus_tag="ACG9CY_000428"
     CDS             45087..46277
                     /gene="pgk"
                     /locus_tag="ACG9CY_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS4936968.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            46381..47136
                     /gene="tpiA"
                     /locus_tag="ACG9CY_000429"
     CDS             46381..47136
                     /gene="tpiA"
                     /locus_tag="ACG9CY_000429"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS4936969.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            47252..48550
                     /gene="eno"
                     /locus_tag="ACG9CY_000430"
     CDS             47252..48550
                     /gene="eno"
                     /locus_tag="ACG9CY_000430"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS4936970.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            48949..50088
                     /locus_tag="ACG9CY_000431"
     CDS             48949..50088
                     /locus_tag="ACG9CY_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS4936971.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        16697 a         8656 c        10625 g        14152 t
ORIGIN      
        1 gctaagtgat tagaatttgt atggatctgt gataactgtc acagatcctc tttttcaagg
       61 atgagaagga aacttctgct gttaaaatca gaaatgcttg aaggagtgga cttggaactt
      121 ttcagtttgc ttgaaaaaaa tgaacaacgg caaatcaaac ttttccaact tttattagag
      181 gaaaaaggag aggtttccgt taataacgta cttacccgct tatcagttga tcgggctacc
      241 ttgaaagaag atctgtttta tttacaagct aacttggaaa atatcaaaca attggaaatt
      301 gttatccaag aagggaatat tttattaatc agacatggaa atatagcagc agtcgatgtt
      361 tattatcatt gttacttgcg ctattctaca aaatatcaga tacttcttta tctgttggaa
      421 aatggctcat ttgatcggca gaaacttgtc atggaactta atatcagttc ggctactttg
      481 tctcgcagag tgaaggaatt aaatgattct ttaaatgagt ttcatctgaa aataaaaagc
      541 gggcggctga ccggtccaga aagtcaaatt cgttattttt atttccaatt attttggttt
      601 ggacgtccct atcttgaaaa caaaaaagaa tacagtgatt tatcaggaaa cactttttta
      661 gatctgctgc aaaaagagtt tagttttgct attacagaag aaggacgatt gaaatttgaa
      721 atatggcttt ccattatgaa gaagcgaatg cgctgtacga aaaaatccaa accagattat
      781 agcaatcttc ctgttggatt gtttcaaacg acacaatttt tattggacct ccatacattt
      841 ttgacccgtt acttctttca atatgccttt ccttggaatc aaaatgaaac gttggttttt
      901 tatcttttca tgatcagtaa ctttacatta gatacacata actcgcatgt ccaggagttt
      961 ttgataagcg aaaaatacaa agacgacgta gtagctcaat tgaatcgaac gtttaaacaa
     1021 tcgatagaag aatatttctc tttttatgtt tttccagaag agatttcgca aaagattgcg
     1081 ttgacagtga tgcaaaatca cgctcatttt gcctatttta gaggatggat gaatattttt
     1141 ggccaagaag gattaaaaca gcggttaact gaagtagaag ataaacaatt cctggtttta
     1201 tgtgaaagat tggtcagaca aacatttgct attgttgatt ttactgaagt agacgaggaa
     1261 aatgcccgct ttgaattatt tgctcgctat gcatctattt tacggacgct gatcaagcag
     1321 ttagaattac gtctgcaaat tgcttatgat cttccttatg aaaggctaat ggcggatatc
     1381 gtgaaagaaa tcatccagga acaattgctg aatacgatca aatatgattt agtcgaatac
     1441 gagaaagaaa agcagtatga tatcattttg actagccaat tgaaagagta cccgtcacag
     1501 aagtccgcta aagtttttgt attcactagc aacgaatata attatgattt cccctatttg
     1561 aaccaatttt taaaagaatg ttatttagaa aaattgaatt taaggatgaa taaaacatga
     1621 aaatcgtgat cattggtgct tcccatgaag gcatcacagc tgccctaaat ctaagaaaac
     1681 ttcaaccgaa agcagaagtt ttactgatcg atgaagatca taaagatgga ctaggctatg
     1741 tttcaaatgg aatcaacctt tatttaaaag gaaagatacg tagtttagct gaagttgccc
     1801 ataacatgcg gactcttcaa gaaagtgggg caaaactgat caccgaatgg agagtaactg
     1861 aacttgatcc agacaaacat cagctcatcc tcgcatccaa agaaggaaaa aacgagacca
     1921 taacctatga taaattaatc gtagcgacag gttcctctcc tgtcaccttg tataaacaaa
     1981 ttgaagctga aaatgtctac acctataaaa atctagtgca atctaagcaa gtacttgccg
     2041 ctttaaaaga agcaaaagaa gtagtgatct tcggtgctgg ttacattgga ttggaattag
     2101 cagatgcttt gagaaataaa ggatacatta ttcatctagt tgattatatg ccgaatgttt
     2161 tatcaagata tttcgataag gatatgatca acagttttca aaatcagctg caaactaagc
     2221 aaataaattt ttatccaaat gaattcttga tcgattggaa aaaatcagaa gagaaagtag
     2281 tatccgtaca acttctgtct caaacaataa aggcagatat ggtcattttt tctgcccaaa
     2341 ctcgtcctaa tacgacattg ttaaaagaaa aagtggcttt gtatgaggat gagactgtga
     2401 tagtcaatga atatctgcag acaagtgatc cggacatcta tgcgattgga gatatcgtcc
     2461 cagtcagttt tgacaaaaat aaacggcatc tctttttacc tttagttact cgagctgtac
     2521 atatggcaag agcagtagcg ctaactttga gtggtcagcc aaccgcttac gacctcagac
     2581 aaaagataac agcagcagtc attatagact actttttagg aactgtaggg ctgacagaag
     2641 atgaagcgcc attcttggaa caaaataccg gttcgtgttc aggagaattt gatctatttc
     2701 cacaatacga cgaagaaaac aaaacagtaa atgcaaagct gatctaccat ccggatacat
     2761 tagaaataat tggcggacag ctgataagcc aagaattctt attatcagat ttgaacctgc
     2821 ttgcggatat cgtgaaacac aaaactacga ttccacagct agcagttgag gattttggat
     2881 ttctgtctga atacacctct cgttttcact acctcaatga gttagctttt aaagtactag
     2941 ctaagagagc aaatagcaat agagtagata aaagactgtg agaaatatca cggtctttta
     3001 ttgtgtgggg atataataaa tattaatcaa tcgagaataa aaaattcata taatgaaata
     3061 gcggaagaat tttttgagca taatattcgc caaaaattta tcaagtgata aaattgttgt
     3121 ataaaatgat tatttcagga ggaggagtta aatgttttta gcattaaatg aaattatgca
     3181 ttcaaagtta cgttatgcgt tagttgcagg ggtgatgttt ttgatcgcct acctggtatt
     3241 ttttttgact gggttagctt atggcttagc tcaagataat cgaacagcag tcgacaagtg
     3301 ggaggcagat tcgattgtgc tttcgaaaga tgcaaattca aatctaggga tgtccatgat
     3361 tactaagaag atagcagaag aagtcgaagg tgggaaagtg gcgtatcttg cccaaacacc
     3421 tggagtcgtg actagtaaag acagtacaga agaaggaaaa atcaatgtta gcttcttcgg
     3481 tatcgataaa aatcaattca tcatgcccaa tcttgttgaa ggaaaagctt ttgataacga
     3541 tgatgaggca gtaggcgata ttagtttgaa agaagaatat ggtctagcca taggagatac
     3601 agttaagtta tctggtagtg acaagacgtt taaactaact ggctttacgg atcatgccaa
     3661 attcaatgtc tcaccagttc tctatacaac catcaatgct taccaagaaa tccgcttcga
     3721 aaaagaagat acaagtgaaa atgcacggat taacgcaata gttgtcagag gtaaaatcag
     3781 taatttacct gaagatttag aacagatcaa aatttctaaa ttcatcaatg aattacctgg
     3841 atataatgcg caagtattga cttttggatt tatgatcgga tttttgatcg ttattgcggc
     3901 tattgtgatc gggattttca tttatgtact gacgatgcaa aagattaata tctttggtgt
     3961 aatgaaagca caagggatca caggaggatt tattgcgcgt tctgtagtgg cacaaacatt
     4021 cattttatca tttgttggta ttcttcttgg tctattagga acagtgggaa catcccttgt
     4081 tctacctgat gcggttccat tccaaagcaa ttggctgttt tttggcgtca ttagcttgtt
     4141 gatgttagta gtagctgtgt taggcgcact attttctgtt cggacaatcg taaaaatcga
     4201 tccgttgaag gcaattggat aagaaggagg aagaacatga aagcaataga atttaaagaa
     4261 gtagaaaaaa gtttccaaga tggcgaccaa gtagttaaag cattgaaaga aacaaccatg
     4321 agtatcgata aaggggaatt tgttgcaatc atcggtcctt ctggttcagg taaaagtact
     4381 tttttgactt tggcaggcgg attgcagacg cctagcagtg gctctgtatt tatcaatgat
     4441 caagaattca gtaatcaaaa agaaagcgaa cgcgttaaac tacgttttaa agaaattggc
     4501 tttattttgc aagcttccaa tctggttcct tttctgacag taaaaaagca gttattatta
     4561 gtagataaga ttaaacatga acatcgtcaa aaagaagcaa acgaattatt tgagcagctt
     4621 ggcgtggata agttgatcaa caagtatcca gaagaactat ctggtggaga acggcaaagg
     4681 gtcgcaatcg ctcgtgcatt gtacaatgat ccgtccatta ttctagcaga tgaaccaact
     4741 gctagtttgg attcagaaaa ggcacgagaa gtagtcgata tcttggcaaa agaatcaaaa
     4801 gacaaaaata aagcaacgat catggttact cacgatacac ggctgattga caaatgcgat
     4861 agaatattta tgatggaaga tggtgtattg aaagaaaaag aataaccaaa aagcaggtgg
     4921 aaaaatggtc catcgataca acaaagatac acagcaaaaa attttgaaag aagcgactga
     4981 cttgtttatg tccaagggat atctcggcac ttcaacaaga gaaatcgcac aaaaagttgg
     5041 gatcacccag cccaatctct atcattattt cggtgataaa gaaaaactat atacggcagt
     5101 tttagaaaac cacttaaaag atgttggaaa agctttgaga gaaatcgttc aaacaagcga
     5161 aacgggattt caagtaacct tgacaaaaat ggcgcagttt ttgattgaga cacatctggt
     5221 cgatttgttt ctgatgcttc acgatcttga aagcaatctt tcaaaggaaa caagagatca
     5281 tcttttcttt ctatggaaga aaaattatcg tgaacctttt gaagaaatct tttcagcaaa
     5341 ccaatctgtg ctaagaaatg ggatttcaaa agaaatcgca gcaaggcatt ttttcttagt
     5401 gttagcacct tacatcactc ggtcgaaaag acctgtggaa gaaccgctaa atgtggcgca
     5461 attgatcgat ttatatttga atggtgtatt aggagaagcg taaagctgtt atatagggag
     5521 aaaatagtta ggtgaagaag caagatatga cgaagatcgt tatatcttgc ttctttatat
     5581 aggtagtttt tcataaagac aaatttgtta ttctctcatg attatcaggg tatattttta
     5641 tttattatgt cggtgttcca ggaagtgttt taatttaata acgaagaaat taattgtttg
     5701 tagaatgaag gaaactattt attgggtaga tgaatcacca cgaatagtca aaaggagatt
     5761 tcaggatatg ccaaaaatga aaaaaatcat tttagcagta atgttatttt ctacggttat
     5821 atcttttaca gccccttttt tgcttactta agctgatttt aagttttggc ccttagtctg
     5881 gatctacgag gtgagagtca agaatgaaaa aaataattgg agcttttgta tttgcaatca
     5941 ttatcattat tatctgtttt caaatagatg tttttggtca aaaaaagata gttggacaaa
     6001 cggtcagcag tacgaacagt acggttacaa agacaaagga atcatttgat gatacagaac
     6061 aagagacatt aatcaaaatc ggaaaaaatg accgtacact atatacagat gactcgctaa
     6121 ctaccccact tgcaactata aacggcggag agctggcgga gtttctttct gaaacaaaaa
     6181 atgcttatca gatcaagaca aatgatggct atactggata tttagccctc gaagatggaa
     6241 ccaaagtcac aaagaatata caaacaaaac cggaagagct atctgatgca gtgattgtat
     6301 tagatcctgg gcatggcgga aatgatactg gggcattaag caatgatgaa caaacagaag
     6361 aaaaagatat aacattaagt acagccataa aagtaaaaaa ggcgctagag gatgctggag
     6421 caacggttta tttgacccat actacagatg aattagtaca gcttggagat atatgcgact
     6481 acagtgaaga aaaaaaggca gatgtcttta tcagtttaca cgcagactct acggaatatg
     6541 cgaatgaagc aactggaatc acgacatatt attactatgg acaagaggaa acattagcac
     6601 agacaattgc tgactccttc actgatctgc ctctggattc aagaggaatc tccactggga
     6661 actatcaagt tcttcgagaa aatcttcaac cttctatttt aatcgagatg ggttatatga
     6721 ataatgatag tgatttaaag gaattagtaa cagacagtta tcagcagcag attgctgcaa
     6781 gtttgacaca agcattgacc acttattttc agtaataaat aagcccagcc aaacaaaaat
     6841 agctcctcat atttttcgtt tggccgggct tattatcgca gatgcaagct tttgaacacc
     6901 gtttattttt ttagtagctt caaagctttt tctgtatttg caaagtgaag tttggcatat
     6961 ttttcaagca taggcatgcc gcctttctcc aatagttttg ccgctacttg gtcagatttc
     7021 gttccagctc ctgatggaag ggtgattcca gcttcagctg atgctttgtg taactcttcc
     7081 aaaaagctgg cacgactgat gatagaagca gcagcaacag ctaaatgata ttgttctcct
     7141 ttagtaacga agtacagttt ttcttgtaat tgattttttt ctaaacgcgc atatttttta
     7201 aaatttgctt ccggcgtaaa ctgatcgatc aaaatcgctt ctggtttttc tggctcgatt
     7261 ttttttagca gcaaatggat cgcttgattg tgcaaagcga ctttcatccg aacggcattg
     7321 tattttggtt ggatctcatt gtatttttct ggagataaga caagcagttt ataggggatg
     7381 gtttttttta agatgtcagc aagctggatg atttggctat cttttaactc ttttgaatct
     7441 ctaacaccaa gtttacgtaa ttctgcaagc tgatcacgtt tcacataagc agcacatact
     7501 gttagcggac cgaaatagct gccatttccg acctcatcac tacctaaaac ggataattga
     7561 ctgatatttt ttggcaaaga agaggctgtt tttgctggct tggcttgttt atcctgtgcc
     7621 tccccccatt tatcagcttc tattgctgca ttcgtacctt gaaacatcac ttttccggat
     7681 gtataagcgg taatggtcgt cccgtctttt tttgcagcaa agctagtata aggaatctgt
     7741 ttatttaaca aataagagcg atagtatgct ttcatttgac taagttggtc gtttgttgct
     7801 ttgataatga cgttgctcat aaaattctcc tcgtttttat acataaaact ataccgcaac
     7861 tcacgacttt tcgttttcta agagattgcg agacatagca tttttttaaa gtttatcata
     7921 taatagttaa gtatgtgaaa aatatttgtg gagaacggtg aaagaaccaa cttcatggta
     7981 cactaaataa aactttcatc aaataggagg aaatgattcg tatggcgcac gaaaaaacac
     8041 gctacaaagc ggtgattgcc aaccaaacat atactattat cggacgggaa accaagcatc
     8101 atatggatat cgtaacgaag ctgatcaatg aacagttagc agaattgaaa caattatcac
     8161 cacaaatgga taatgaacaa gcagcaatct tgatggctgt caatgcgctt tcggatcaat
     8221 tgaagaaaca agaaagaatc ttggagctgg aagaagaaac agcagaatta aagaaaaaaa
     8281 tgatcaaatt tacagagctt gaaaatcgtg tgaaacgcat tgaagcaatc gagaacgaag
     8341 ctcgggaagt attgaaagaa aatggtcaag cagatcatac cattaaaaat catgtggaag
     8401 cacaacagat tttaaacgag aaacgaaaag gacaaattaa acaaaaatct tctaattaag
     8461 atccttttat caaaataaga aaaatttaaa aaaagttaga agagaaagga acgtagcatg
     8521 ctgagtttgc tgattttatt tttattgcta attgcgtttt tttctggagc acgtcgcggg
     8581 tttgctttgc aaatcatatt cgcgattgga tatgtattgt catttattgc tgcccagcat
     8641 ttttacaaac cattagcaag tcatttggaa ttatacattc catacccagc tgtcacgcca
     8701 acatcaaagt tagcattttt tgatcaggta tttgcctttc acctagatga agctttttat
     8761 gcaggaactg cttttctgat tattttgcta attggctggc tgcttacccg atttgtgggg
     8821 gtttttgtcc atggattgac gtatgtgccg atcttgagac aggtagactg gattgcagga
     8881 ggcatcctgt cactaatcat ggcttacgtt actatatttt tgattttgca gttacttgcc
     8941 tttgttccat tagatgtggt ccaaaatcaa ttcggcgagc atagtttcgc tcgctttatc
     9001 gttgagagaa cgccatttct taccaacaag atgtatgagc tttgggtaac gaatattttg
     9061 aattaaggaa aatagtgctg gaaaatattt cgttttttgt acagagtaga ttatttaaga
     9121 aggctgcgta gcgtatgtcc ggcacagctc ttttttcata gtgaaagata aggtgataaa
     9181 atgaataacc gaatccttga aacattggaa tttgaaaaag tcaaacagat ggtcagacaa
     9241 tttgtagtga ctgcacaagg aaaagaagaa ctggcagaat tggtaccagt gagcgaggaa
     9301 caaacgatca caaactggct tcaagaaact gaggatggat tgaaagtgca acgtctgcga
     9361 ggcgggatcc ctatacctaa acttgaaaat atccgtcccc atatgaaacg gattgagatc
     9421 ggagcagact tgaatggagt ggagcttgca caagtttcca gagtattgtc tacgacaagc
     9481 gaattgaatc gtttcattga tgatctttat gacagtgaaa tcgaatttgc acgtttgtat
     9541 atgtgggcag atcagttagt cacgatccct gttttgagcc gccgtttaaa ggaagcaatc
     9601 gatgaagacg gacgcgtgac tgatgacgct tctcctgagt taaagagcat aagacagaac
     9661 attcggcgaa gtgaacaagc tgttcgtgaa cagttagatg ggatcgtccg tgggaaaaat
     9721 gcaaagtatt taagtgatgc gatcatcacc atgcgaaatg accgctacgt tatcccagtc
     9781 aaacaagaat accgcggtgt atttggcggt gtagtccacg atcaaagtgc ttcaggacag
     9841 actttattta ttgaaccaaa acaagtggtg gatttgaata atcgtttgcg tcaataccaa
     9901 atcgctgaac gaaatgagat tcaacgtatt ttaagtgaac tttctgctga acttgttccc
     9961 catcgtcaag aaatcatcca caatgcttat gtgattggta aaatggatct gatgaatgca
    10021 aaagcacgat ttggtaaaga agtaaaagct atcgtgccgg gaatcagtga agacaatcat
    10081 gtcgtcttga aacaagcaag acatccactg atcgatcagg aaaaagtagt ttctaatgac
    10141 atcacaatcg gaaaagacta tcaagccatc gtcattacag gacctaacac aggtgggaaa
    10201 acgatcacgt tgaagacttt aggtctgtta caactgatgg ggcaagcagg attaccgatt
    10261 cctgcaggtg aagaaagtca aatcggtatt tttgaggaag tatttgcgga tataggagac
    10321 gaacagtcga ttgagcaaag tctatcgacc ttttcttctc atatgacgaa cactgtagat
    10381 attttatcaa aagtcaatga aaaaagtctg gtattatttg acgagctcgg tgcaggaaca
    10441 gatccgcaag aaggtgctgc tctagcaatc gcaatcttag atgatcttgg taaaaagtct
    10501 gcttatgtga tggcaacaac gcattatcct gaattgaaag tttatggata taatcgggca
    10561 aatacgatta atgccagcat ggagtttgat gtggatacat taagtccaac gtatcgtttg
    10621 ctgattggcg ttcctggacg aagcaatgcg tttgaaatct ctagccgatt aggtcttgat
    10681 acagaagtta ttgatgaagc gaagcagttg atgaatgatg agagccaaga tttaaacgaa
    10741 atgatcacag atttagagaa tcgccggaaa atggcagaga ccgaatatct ggaaatgcgt
    10801 cattttgttt ctgaggcaca agaacttcac gatgatttga aagaagctta cagctatttc
    10861 tttgaagaac gtgaaaaaga aatggaaaaa gcaaagaaaa aagcaaatga agtcgtgtca
    10921 gaggcggaag aaaaagcaga aaaaatcatt gctgatattc gtaaaatgca acagcagatt
    10981 gggcaaggaa atgtgaaaga gcatcagttg atcgatgcaa agacccaatt agcaaatcta
    11041 catcaagaag aaactttgaa gaaaaataaa gtattgaaga aggcgaaaga acaaaaaacg
    11101 ttgaaaccgg gagacgaagt gttggttacg acttacggcc aaagagggac gcttttacgt
    11161 aaaaacggga atcagtggca agtagaaatc ggtatattaa aaatgaatgt gtccgaagac
    11221 gagctgacgc ctgttgctcc tcaaaaagag ccgacacaac gagttatcca tgctgtacga
    11281 tctgaatcta gtagtcacgt gccgaatcaa ttggaccttc gaggaaaacg atacgaagaa
    11341 gcgctaagtg aggtagatca atatttggat tctgccattc tagctggtta tcctcaagtg
    11401 actattgtcc acggaaaagg aacaggcgca cttcgcaagg gaattactga ttatcttaaa
    11461 aaccatcgga gcgtcaaaag ctttgagttt gcaccggcaa accaaggcgg aaatggtgcg
    11521 acaattgtta aatttaagta aagcttacta aaggtaagca gatggttcgc tatccaagta
    11581 tagaaatgct atactagtca tagattttaa aaatggaggt ctttgatatg tcacaagtaa
    11641 ttacagataa gacatttaat gaagaaacag ataaaggact tgtattgatt gatttttggg
    11701 caacttggtg tggtccatgt cgtatgcaag caccgatttt ggatcagttg gaacaagaat
    11761 atgatgaaga agaattcaga attgctaaaa tggatgtgga tgaaaatcca gaaactcctc
    11821 aacaattcgg tatcatgagc attccaacat taatgttgaa aaaagatggt caagtagtag
    11881 aaaaagctgt tggtgttcat tcaaaagaac aactacgtca aatgatcgat caatatttat
    11941 aaaaacttac agcaaaaata agctgagggt gggactgaat ccagttccgt cctcggtttt
    12001 tttgcggttg agttaaggtt attcaaatta atattttttg agtataatta cgttgtcagg
    12061 ctggattgtt tttttgacag acatgctata ataactttaa ttgaatatga aggggagtac
    12121 cagatgaacg aacgaatcaa aaacaaatta gctttacttc ctgatcagcc cggctgttac
    12181 ttgatgaaag ataaaaatgg aacgatcatt tatgtcggca aagccaaaat attaaaaaat
    12241 cgtgtccgct catattttac tggtagccat aatacgaaaa cggaacggct tgttagtgaa
    12301 atcgaagact ttgaatacat cgttacagaa tcgaatatcg aagcattgct cttagagatc
    12361 aatctgataa agaaaaatga tcctaaatac aacattatgt taaaggatga taaaacctat
    12421 cctttcctta agataacgaa tgaaaaatat ccccgtttgg tgattactcg taaggtacta
    12481 aaagacaaag cactgtattt tggaccatat cccgatgtcg gtgcggcgaa tgagacaaag
    12541 aagatcctcg atcgattatt tcctctgaga aaatgcaaac ctagtcagac gaaggagcct
    12601 tgtctttact atcatttggg acaatgtctg tgtccttatt atttcgacgt agatccgaaa
    12661 gtctatacga aaatcgttga tgaagtgaaa aactttttaa acggaggaca cgaaaaaatc
    12721 gagtcagaga tccatcagaa aatggaaaaa gctgctgaaa acatggagtt tgaaaaagct
    12781 gcagaatacc gagaccagat ccgtgccatt gagacaatca tgactcgcca aaaaatgaca
    12841 aatactgatt tactggatcg agatgtattt ggttatgcga ttgataaagg atggatgtgt
    12901 gtccaagtgt ttttcgtaag acaaggaaag ctaattgaac gagatgtttc aatgtttcct
    12961 ttctataacg aagcagaaga agatttcttg acttacctcg gtcagtttta tcaggaaaat
    13021 gagcatttca ttcctaaaga agtcttgatt cctgatacga tcgacaagcc cagtgtagaa
    13081 gctttgttag ctacgaaagt gctgcaacca aaacgaggag aaaagaaaaa attagttgaa
    13141 ttggcgagta agaatgcctc agttgctttg aatgaacggt ttgatctgat tgccagaaaa
    13201 caggaacgga cgatcggagc agttgaacgt ttaggaaacg cgatgaatat cccgacccca
    13261 atacgtatcg agtcttttga taactctaat atcatgggga cgaatcctgt ggctgcgatg
    13321 gtcgtattca ttgatggaaa accggcaaga aaagaatatc ggaaatataa aataaaaaca
    13381 gtcaacggac cggatgatta tgcatcaatg agagaagtca tctatcgcag atattcgcga
    13441 gtgattcgcg aagggttgcc tttacctgat ttgatcttga tcgatggagg aaaaggacaa
    13501 gtagatgtgg caaaagatgt cttagaaaat caattaggaa tcgatattcc tattgctggc
    13561 atggcaaaaa acgataaaca taaaacaaat gagttgctat tcggtacaga tttggctgta
    13621 gtaccgcttg agcgaaattc accagagttt ttcttactcc agcgaatcca agatgaagtc
    13681 catcgattcg caatcacttt ccatcggcag ctacgcagta aaaacagttt tgcttcccga
    13741 ttagatgaaa ttgaagggtt aggaccaaaa cgaaaaaaaa tgctgcttaa agagttcaaa
    13801 tcgttgaaaa atatcacagc agccacgaca gaagaattac aagaaatcgg actgccgaaa
    13861 aatgtcgcac aaaacgtatt tgataaatta catcaggaat gaggaaaggg cactttacaa
    13921 agaaagtaaa gtgtcctatc attttatgta caacaaaaag agcgtttacg ttttgttggt
    13981 aaataataaa tcgcttacgt ctggtgtcta aggatatatg tgatttcttg aagaaggagt
    14041 gaagaaatgt tagaagtcaa ggatcttgtt aaaacttttg ggaactacac agctgtcgat
    14101 catgtctctt tccaaatccc cgatggaaaa atcatggggc taattggcca aaacggggct
    14161 gggaagacaa cgacttttcg tttgatcttg gattttttga cacaagacca aggagaagtg
    14221 ttatggaatg gccatcatct tggggagaaa gattatgatg tcatcggtta tcttccggag
    14281 gaacggggac tttatccgaa agtaacgatt caagaccaac tgctttattt tgcagaactt
    14341 agagggaaat caagaaaaga gattgaacca aaaatcgact tttggatgga aaaatttcag
    14401 gtaaaaggga aaaagacgga taaagtaaaa tctttgtcta aaggaaatca gcaaaaagtc
    14461 cagctgatcg ccacgttaat ccatgaacct aaattgatca ttttggatga gccattcagc
    14521 gggttagatc cggtaaatgc tgaattgcta aaagatggaa tattggaatt gaaaaaacat
    14581 ggttcatgtg tcattttttc tagccacaat atggataatg tagaaaaaat ctgcgatcat
    14641 ttgatcatgt tgcgaaacgg aaaaatggtt ttgaatggaa aagtacatga gattcgtgaa
    14701 tcatttggcc gtacgaagtt attcttggaa tctggtttaa gccaacagga agtagaagaa
    14761 atcgaaggcg tgaaaaaagt gatccttcat gaagacaata cgttagagat taccttagaa
    14821 gatcctgaag ctgggaaaga aatctttact cgtgcgactc agttcggcta cattcctatg
    14881 tttaaccagc aaccgccaac tttggaagag attttcaaat tgaaagcagg tgaaatcgat
    14941 gcgtaagttt tggattatta ccaaagatgt ctatttaaaa aatgttaagt cgatttcttt
    15001 cttgatcatg atattagtcc cttttatcat gctgggtatc atttatgtag caggaaactt
    15061 tgctcaacaa aatagtgatg tcaataaaat cggcgtattg gctgataatg agcaaatcag
    15121 tcagcaaatg agccaagtca aaacagatga ttttgagttc aaagcagttg attctgaaga
    15181 aaaagcacaa aaagagcttt cggaagaaaa aatcgatgcg tatttagtag tctcaactga
    15241 gaatgataag ataaatggga tgctttatag cgaaaattca ttaggacaaa gtacacaact
    15301 actgatacaa caacaactga ctggcttgca atcgatgctt cgagcgagtc aattaggcat
    15361 ttcaccagaa gaagtagcta gcttgagcca acaagcaagt ttgactcgac aaaaagtcaa
    15421 ttttgattca aaaggaaaaa tgacagtcgg agaagataac tcgacagtcc aatacgtcgt
    15481 aagttatgta gcaacgattg tcttgttcat tattattttg acgtatgcgc agatcattgc
    15541 acaagagatc gcttctgaaa aaggcacgcg gatcatggaa gtgatcctgt ccagtacgac
    15601 tgcacaaaag catttttacg gaaaattaac aggtgtttta cttgttgcat taacgcaaat
    15661 ggcattatac ggtattattt ttggtatagg atacagtcaa ttcaaagata tggaaatggt
    15721 caaaaatata ttagacggta tctcattgga taacttgatt ggaccattct tgtggtattc
    15781 tcttctattc atgatctttg gcattttgat tttttctgtt ttagcagctt tatgtggctc
    15841 tttggtcaac aaagcagaag atacagcaaa agcaattttg ccagtgactt atctatctct
    15901 aggcggatac atgcttggat tgatactagg agcttcagat ccaaacaata tcgttattcg
    15961 gatcacttcg tatattccat tcttatcttc atatattatg ccggttcgct tagcaaatga
    16021 gacagtcggt atgggtggag cattgatttc cttgttggta cttgtagccg taacattctt
    16081 attgatgatc ttgtctgcaa acatgtacaa atcgaatgta ctcgtttata gcgaaggcgg
    16141 attatggagt tcattgaaac aatcgatttc aattatgagg aatgaacgaa aaaaagcata
    16201 aaacaatcct aaaatacaga aggaagctgg acatctattg ttcccagctt ctttttgtat
    16261 agacctattt tatttatttt actatattgg atatatattc attgaaaata gtgtataaca
    16321 actttataat attgagtata ccaatttttg gaggcgtttt aatggaccgt tatttagatt
    16381 tactttcaga aaaattcccc tcaacggagg atgtcatcac ggaaatcatc aatctcgaag
    16441 caattttgca gcttccaaaa ggaacagagt tatttttgag tgatatacat ggcgaatttc
    16501 ctgcttttga tcatattctg cgtatcggtt caggaaatct taaagaaaag atcaaagacc
    16561 ttttttctga acaaatgacg gaaacggata tcagccaatt cactattttt acggcttatc
    16621 cagaatatgc attaacgact gattggtaca aagaacaaga taaatcgcag ctgatccatc
    16681 atttgatcga tcttttacgg tttacgacag tcaaatatac ccggtcaaaa gttcgaaaag
    16741 gattgccgaa agaatatagc tatatcattg aggaactctt gtatatcgat gaccgtatca
    16801 atggaaaaaa agactatgta aaaaaaatca ttgaacaact tgttttgatg aaggaagaag
    16861 aaaaattttt aacgaaactt gcacagacga tccaaaaatc tgtcatcgat catttgcata
    16921 tcgttggaga tatttttgac cgaggaactc aagcagccaa agtgatggat cgaatcatgg
    16981 atttttcttc tgtagatatc caatggggaa atcatgatat tttgtggatt ggtgcgtatt
    17041 gcggctctga ggcatgttta ctgacacttt tacggattgc agcacgctat aattatttat
    17101 ttgaattaga gaaagaatat gggttgaatt tacgtccatt gttttctttt gctcatcaaa
    17161 cttatcagaa gaatccagta tttgcgccta aaaacagtaa aaatctatcc gaaagagagc
    17221 aagaagaatt agaaaaaatc catcaggcac tttctatcct tcaattcaaa tcagaacatc
    17281 agttattgga tcgtcgacct gaatttaaaa tggacgatcg taaattgctg gaaaaaatcg
    17341 actatgaagc atcaacgatt gatttagaag gtcagctcta tgagttgaaa ggaacttgtt
    17401 ttcaaacgat ccagccagat gatccaaaaa aattcttgga agaggaacaa aaagtgattg
    17461 atagtttgat gtattcattc cagcactctt tgagaatgca aaaacatatg acgtttttga
    17521 tcgaaaaagg gggaatgtat ctcacgaaca atcagcatgt gctgtttcat ggctgtatac
    17581 ctgtagacga gaagggacag ctcttagcat ttgaattaga ccaatcttat cgcggaaaag
    17641 aactgctggg cttttttgaa aaacagataa ctgaaagcgc aaaagatctg actgcaaaag
    17701 aagatttagc aaccgatttg atttggtatg cgtggagtgg gccattttcg cctttattcg
    17761 gcaagagtaa aatggcgact tttgaacgtt attttctcac agagcctcat acgcacgtcg
    17821 agaaaagcaa tccttattat cacttgcgag atgaagagtg gttcagtgaa aaaattttag
    17881 cagaattcga cacaaaagaa gaagggtcag caatcattaa tgggcacacg ccagtaaaag
    17941 taaaaaaagg ggagtcaccg atcaaagcaa atggaaaagt atttgtcatt gacggaggtt
    18001 tatccaaagc ttatcaaaaa acgacaggaa ttgccggtta ttcactatta tgcaattctt
    18061 atggtttcca aatcgtgaca cattatccgt ttttgcctat tgaacaacaa tttgcaaaaa
    18121 aatccgacca aacaaatgtg aaaaaagtaa tcgaacagca actaccacgc aaactcaatc
    18181 gtgatacaac aaaaggtatg gaactgcagc agcagattac ccagctcaaa aggctgttgg
    18241 actatcgaaa ggacgattga gaagaattcc agtgaacatt gttccataga ctggagactg
    18301 agacaaaagt cgtgtcgcag tctctttttc cggataaacg gtgttcttct gccaactcct
    18361 cggtcacaag tcacactatt caacaaacat gaaaagctgt tttttgaaat tgctccttgt
    18421 gactcagagt tgaacggcag gctcacgacc tcttggtata aacggtgttc aaaagccagt
    18481 ttctcggtat taagccaaat ttatgcaaaa atgtgaaaag ccattttcac aaaatttttc
    18541 ttaatactcg aaactaaaca gctttttcac aaccccctca gaaagtctat taaaattcat
    18601 tcacaaagaa atcgaataag cgctgttcag atggatgtac acggtgggta agttccggat
    18661 gccattgaac accgaggatt ttatgcatat catctttgct ttgaaccgct tcaatgagtc
    18721 catcgttaga aaaagcgatg gcttttaata aaggtgaaag atctttgact gcctgatgat
    18781 ggtaagaatt cacaaaatat ggatcgggga gaaggttgct taacaaactg tctgaaacaa
    18841 tcttgacttc gtgagtcgta aattgcgggg cagtgggctg ttgttcatgt tttactgacc
    18901 actttggata taatgaaaga tcttgataaa gtgtaccttc caaaacgaca ttcaatagtt
    18961 ggagtccccg gcagactgca aaaattggtt ttctttgttt caatgcttcc aagatcagtg
    19021 cctgttcaaa ttggtctcgt tgaatatttg tttccgttag ctttggatga ggatcttcca
    19081 tgtaaagttg tggactgaca tcttgaccac ctgcaagaat aagtttgtcg atttgtccaa
    19141 tcagttgagg agctgaatct ggagacatga ctggcagaat aagaggcaat ccttgagcat
    19201 ctaaaactgc atccacgaat ccttgcggtg tatagctcac ttgatttcct tggaaagtat
    19261 ctgttgctcg aatcagttga tttccggcaa tgccgatgat tggtttcatt tgtattcccc
    19321 ctaaagtaga cttattgtaa caaggattta aaaaaaatta aactatgtaa gacttgcccc
    19381 tatacttatc tgttcttgct gtgttactat ctgtaaagag aaatttcgtg tgaaattcat
    19441 gacgatagtt gaaaatattg cttaatttgt tataataaaa agtaagcaga aataaaaagg
    19501 ttgggcgtaa gcttcaagga tctagttctt caaggggtga gaaggacatt ttattgaagg
    19561 ggcctgccta tactaagtta tctttagagg tacatacttt caatgaaaaa agaaagcatg
    19621 tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta gaagctttgg aacaaatccg
    19681 aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg cggcagcagc tgaagatcta
    19741 tgcaacagaa aaagagcagg aattggaaac gatccaaaca aactatcgac aacaactaaa
    19801 acagttggtg gaagaaaaag aacaatttga aatacaagaa ttagaaaaag aaaaaatgaa
    19861 attgaaagca gaagcagaca aggtgcaagc aactttgaaa gatcaatatc aaaagcatca
    19921 gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa acttatggcc gtcactaaga
    19981 tggaaaaagt gaccctcatt tcagataaaa gaaatcagga gaggatttta caagcagtac
    20041 aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa tagaacaaac aaccagtggg
    20101 tggagaagta ttttccagat accacgatta ttgataaaga agcaaaatta agcgctttag
    20161 gtaatcggtt gaaagacatc caagaagcta tccaattcat acaacaccac ggcgatagcc
    20221 gtcaaaagaa actccatttg aaacgtcaag aactttcctt acaagatttg gaagccaatt
    20281 attccgaaga aaatttcata aataaattga aagaagtcct tgatttaaaa gaaaagtggg
    20341 aacaactaac tgaagaacgt gatcgtttag aagaagagga agaatggctt cttcattggc
    20401 aacagctgga tattgctcca agtatgtatg caagtaagac gaccattttt gagatgggga
    20461 ctgtgagtgc agcaaatttt gaaagtttca aggaagaact gagtaagctt gatgacgttt
    20521 atatggaaga aatagattat acgccaaaac aagtttatct ttcgtatgtt gctttaaaaa
    20581 aatcagttgc tgttatagaa gaaattgctg ggaaatacgg gttcaataaa gaaacgtatc
    20641 cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa gcagcaatta tccattgttc
    20701 acgagcagca aaagaagata acgacagcgt taggtcagtg cagtggctat atccaagaat
    20761 ttgaatgggt agaagaagtc acacttgccg ttgcagaacg agaaaaaata aaagaacggt
    20821 ttatccatgc gacttatctg atcgttttgc aaggttgggt agatgcagaa gaaaaacaga
    20881 atttgctgca tgtgttaaac gaaaccgtat cagaagaaga tatatacgtc agtttcgaaa
    20941 caccaaactc agaagagatt cagcaggaag tccctactaa attgaaaaac catccgcttg
    21001 tcgaaccttt tgaattgctg actgaaatgt acagtctgcc aaaatatgaa gaagtagatc
    21061 caacaccttg gatgacacca ttttatcttg tcttcttcgg gatgatggtc gcagatgttg
    21121 gttatggttt gctgatgctc attgggacaa tactagctca aaaactactt gttctaccac
    21181 gcgggatgaa acgtttcgta aaattctttg agattctctc gataccatcg atcatctggg
    21241 gattgatcta cagttcattt ttcggacagt cattaccaaa agaactgttt ggcatccgct
    21301 tgcctttccc aatcctatct acgacggatg acgtcaatac gattttgatt ttatccgtga
    21361 ttttcggtct gatacagatt ttggttggat tatttgtggc agcaaaggaa aatatcagga
    21421 gaaaagatta cttgagtgct gtcagtgatg gatttgcctg gcaagggatc ctaattggga
    21481 tcgttattgc gttggttggt gccatgttga taaaaaatac agcatttgtt taccttggcg
    21541 ctgggctatc gatcattgct gccttatgta tcttgatcgt tccgattatc caaacgccgt
    21601 ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta cggactgacc ggctatatcg
    21661 gtgatttagt cagttataca cgtttgatgg cattgggtat ttcaggcgga agtattggag
    21721 cggcattcaa tatgctagtt gctttcatgc cgccagctgc tcgattcagt gtagggatcc
    21781 ttttgatcat cgctttacac gcactgaata tgtttttgac actgctaagt gcctatgtcc
    21841 atggcgcacg attgcagtat gtcgaatttt ttgggaagtt ttatacagga ggcggacgtg
    21901 cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat caatcacaag aaaaaaaaca
    21961 ataaataatt ggaggatttt tatataatga tggattactt aattagtcaa aatggtggaa
    22021 tggtatttgc ggtattagcg atggcaacag cgacgatttt ttcagggatc ggttcagcta
    22081 aaggtgttgg aatgacaggt gaggcagcag cagcattgac aacaagtcaa cccgaaaaat
    22141 tcgggcaggc tctgattctg caattgcttc caggtacgca aggtttgtat ggattcgtta
    22201 ttgccttttt gatttttatc aacttaggaa atgacatgtc agttgtacaa ggactgaact
    22261 ttctaggtgc ttctctgccg attgctttta caggcttgtt ttcaggtatt gcccaaggaa
    22321 aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc tgaacatgct acaaaaggaa
    22381 tcatttttgc tgcgatggtt gaaacatacg cgattttagg tttcgttatt tccttcctat
    22441 tagtgttgaa tgcctaagca taaaggagga aaagcagatg aacgccattg aaaaaatcat
    22501 ctctcagatg aacgaagcag cagagcagga aagagcggcg ttagaacaag aagagcgaat
    22561 gaagatcgat caaaactttg aacaaaaacg tacgcaagtt gaaacagaac atcaaaaaca
    22621 aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt cagctaagga atcgccagca
    22681 agtagaagta cgacaagaaa atttgaatgc gaagcaggaa tttttaagac gattgtttgc
    22741 agatgcggta actgaaatgg aaaattggga tgaatctgaa caaatccagt ttatcaaaaa
    22801 tgcattgtat tcattacctt tgacaggaaa agtagcattc attgctggag aaaaatcagc
    22861 agcctatctt tctcagacgt tgttggatga atggaacaat gaacttccct ttatgatggt
    22921 tttaagtgat gaaactgttg ctgatcaagc gggttttctg ataaacgatc aaggagttca
    22981 gtataatttt cttttcagca gccttgtaca agatatccaa ggaacgatga gttttgagat
    23041 tgccaatcaa ttattcgaat aaggagggat cagtgtatgg aataccacga actaaatccc
    23101 ttgattcgcg gaagagagct tgaacttttg tccaatgaga catttgaacg gatgattcaa
    23161 acaaattctg tcgaagcact tggtgaaata ttgaaatcca cgatttacag tccatatatt
    23221 tatgacggat atgaatatga cttcgaacag aatcttttaa aggagcagag ccgtctattt
    23281 cgctggttaa aggaatcagc accggagcct gaaatcgttt ggatctatac gatgcgttat
    23341 actttccata atctgaaggt tttgacaaaa gctgagatga caaatcaaaa tctggaccat
    23401 ttgtatatcg acgatggatt ttattcatta gaaaccttga aggatgccat tcatacgcaa
    23461 gtttcaaatg aacttcctga aagcattatg gacagtatac gagaagtacg tgattatttt
    23521 gaagagtcta ccattcttca gggaattgac gtgatttatg atcgacagtt tttgacagag
    23581 cagcgacggc ttgccgataa gctaggatat caagaattgc tgacagaagt cattgctttt
    23641 attgacttga ccaatatcac aacgatggct cgaggaatat tgcagaaaag aacatatggg
    23701 ttcatgacga cggtactttc cagctcggga agtatcgaaa aagaagtatt tttgaacttt
    23761 gtagataaag atatggaaga ttacacgaat tttctattag agacagatta tgctgatttt
    23821 ctgcgccctg tcatccgtga gggaaatatt gatctcgtaa agcttgaaca gttgaaagat
    23881 gattatctaa gttcattgta tcaagaagca caaacccagg ctttcggacc actgccacta
    23941 ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc ttcgtttact ggttatcggc
    24001 aaaagaagcc atttcgaatc agaaacaatc agaaagcggg tgagacaagt ctatgacgca
    24061 taagatcggt gtagttggcg acaaagattc tgttttgcca ttcaaattgt ttggctttga
    24121 tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca attgaagaaa tggcaaagaa
    24181 tgagtatggc gtgatttatg tcacagagca atgtgcagca ttgagcccag aaacgatcga
    24241 acgttacaag gaacaaatga tacctgctat cgtattgatt cccaatcatc aaggatcatt
    24301 agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa gcagtaggtc aaaatatctt
    24361 atagtatgaa aaggagatag aaattttgca aattggaaaa atcataaaag tctccggacc
    24421 tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa gacatgtgtc tagtgggaga
    24481 tctaggagtt atcggagaaa ttatcgagat gcgtggcgat gtggcgtcta ttcaagtata
    24541 tgaagagact tctggaattg gtccaggcga accggttcgc tctactggtg aagctttatc
    24601 tgttgaatta ggaccaggga tcatttcaca aatgtttgac ggaatccaac ggccattaga
    24661 tacctttatg gaaatcacac aaagtaattt cttagggcgc ggagttcaat tgcccgcatt
    24721 ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa gccggagaaa cggtgagtgc
    24781 tggagatgtt atcgggatcg tagaagaaac aaaagttata aaacataaaa tcatggtgcc
    24841 aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt tcattcacta ttgatgaaac
    24901 agtatgtgtc attgaaaccg aaaatggtga caaaaatttg acgatgcttc aaaaatggcc
    24961 agtaagacgt agtcggccaa tcaaagaaaa attaaacccg gatgcaccca tgatcactgg
    25021 acagcgagtg attgatacat tcttcccagt gacaaaagga ggagcagcag ctgtacctgg
    25081 tccttttggt gcagggaaaa cagtcgttca gcatcaaatt gcaaaatggg ccgatgtgga
    25141 catggttgtc tatgtgggat gtggcgagcg tggaaatgag atgacggatg ttttaaatga
    25201 attccctgaa ttgattgacc ctaatacagg agaatcgttg atggaacgta cggtattgat
    25261 tgctaataca tcaaacatgc cagtagcagc tcgagaagct tctatctata cgggaatcac
    25321 aattgcggaa tatttccgtg acatgggtta tgacgttgct atcatggcag actccacttc
    25381 acgatgggca gaagcacttc gtgagatgag cggacgtttg gaagaaatgc ctggtgatga
    25441 aggttatcca gcttatcttg gttcacggct tgcggaatac tatgaacgat ctggaagagt
    25501 cattgcatta ggatcagaag gtcgcgaagg aagtataacc gcaatcagtg ctgtatcccc
    25561 gtctggaggg gatatttcgg aaccagtcac acaaaataca ttgcgggtcg taaaagtatt
    25621 ctggggtctt gattccagtt tagcccaaaa aagacatttt ccttcgatcg attggataca
    25681 aagctattct ctttattcaa cagaagtagg gaagtatatg gatcatatcc ttcaagctga
    25741 ctgggcatcc atggtcaccg agggcatgcg tattttgcag gaagaacagc agctgaatga
    25801 aatcgtgcgt ttggtaggga ttgattccct ttcagataat gaccgtctga cattagaagt
    25861 ggcaaaatcc attcgtgaag attatttgca gcaaaatgct tttgatgatg tcgatacatt
    25921 cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc ttgacctttg gtgaagaagg
    25981 acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc atggacggta ccgtatcgat
    26041 tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca gaattagaaa aactagatca
    26101 aattattggt gaaatcaagc aaacgatgca aacgatcgtt tcagaaggag gcatgagcaa
    26161 tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc attgatggcg gttgaaaaag
    26221 ttgctggtgt caaatatgaa gaattaatcg aagtccgtat gcaaaatggc gagattcgtc
    26281 gtggacaagt attggaagtc caagaagata aagcaatggt ccaaatattc gaaggaacaa
    26341 gtggtatcaa tttacgtgac tcttctgtac gatttttagg ccatccttta gaactaggtg
    26401 tatcggaaga catgatcggt cgagtattcg atggtttggg acgaccgaaa gataacggac
    26461 cagagatcct gcctgaaaag catttggata ttaacggaga agtaatcaat ccagtagctc
    26521 gggattatcc agatgagttt atccaaacag gtatctctgc aatcgatcat ttgaatacat
    26581 tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg tcttcctcat aaagaattag
    26641 ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc agatgatttc gccgtagtct
    26701 ttgctgctat tggaatcact tttgaagaag ctgaattttt catggaagat tttcgtcaaa
    26761 cgggcgcaat cgaccgttct gtcatgttta tgaatttagc aaacgaccct gcaatcgaac
    26821 gtatcgctac tcctcgtatg gctttgactg ctgcagaata tcttgcttat gaaaagggaa
    26881 tgcatgtttt ggttatcatg acagacatga ccaactatgc agaggcattg cgagagattt
    26941 ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc tgggtatctt tacacgaact
    27001 tagctacttt attcgaacga gcaggaagaa tccgcggact aaaaggatcg gttactcaaa
    27061 ttccgattct aacaatgcca gaagacgata aaactcatcc aatccctgac ttgactggtt
    27121 atattacaga aggacagatc atcttgacta gagagcttta caagagtggg attcagccac
    27181 caattgatgt actcccgtct ctttcgcggt taaaagataa gggaactgga cccggaaaga
    27241 caagagaaga ccatgcagct acgatgaatc agctttttgc agcctatgca caaggaaaac
    27301 aggcaaaaga attagccgta gttttaggtg aatctgcttt atctgatgta gacaagatct
    27361 atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa tcaaggattc tacacgaacc
    27421 gttcgattac tgaaacactt gatctagggt gggaactgct atcgatgttg ccaagaaccg
    27481 aattaaaacg aatcaaagac gacatgctgg acaaatatct gccagaaggg aagtgagttc
    27541 tgatggcacg attgaatgtg aatccgaccc gcatggaatt gactcgattg aaaaaacagt
    27601 tgacaactgc cacaagaggg cataaattat taaaagataa gcaagatgaa ctgatgcgtc
    27661 aattcatcct gttgattcga aagaacaacc agctgcgtca agaaatggaa gagtctatga
    27721 cagaagcaat gtctgatttt gtattggcaa atgcttcgat cacggaacct ttcatcgaag
    27781 aattatttgt tttgccggcg gaaaacgttg atttgtctat cgtagacaaa aatatcatga
    27841 gtgtaaaagt acctgtgatg aattttgaat atgatgaaca gctgacagac acacctttgg
    27901 aatacgggta tttgaattcc agtgccgaac tggatcggtc aatcgatcgg tttactaccc
    27961 tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac ttgtcaactg atggcgagtg
    28021 aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata tatgacgatc cctcaattag
    28081 aagaaacgat ctattatatt cgtatgaagc tggaagaaaa cgagcgcgca gaagtaacaa
    28141 ggttgatcaa agtaaaaaat atgggtacca ctgactaagt ggtatctttt tttaaaagag
    28201 gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt ttgccggctg ttcagcttct
    28261 cgcattagga ttttttctcc tgattttagt tggcggcggc ctacttaccc taccattttt
    28321 tagtaatagt gggcatggga caaattttat cgatgcgctt ttcactgcaa cttctgctgt
    28381 ctgtgtaacg ggattgacaa cattgaatac agcagagcat tggaacagtg ctggtcaatt
    28441 tttgatcatg cttttgattg agatcggcgg tcttggtttt atgatggttc cgattctatg
    28501 ctttgcattg ataaagaaga aagtcagtct cagcacacgt attatattaa aagaagcgct
    28561 gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt tacatcctaa aattggcctt
    28621 tgtgatccaa ttgattggta cggttgcttt atcttttgta tttgtgcctg aatttggctg
    28681 gttgaaaggt atctggtaca gtttgttcca tgcaatctct agtttttgta atgcagggtt
    28741 tgatttgtta ggagacagtt tagccaatca ccagcaaaat gtttatttga tcatggtggt
    28801 atctgctttg atcatttctg gtgggttagg cttcatcgta tggcgtgatt tactggaata
    28861 tcatcagaaa aagaagatga cgattcattc aaaaatcgca ttaaccgtta caggtaccct
    28921 tttattaggc ggattcgttg tgttcttttt cacagagcgc aatggggcaa cgctagtgga
    28981 agggaatatg tttcaacgat tggcaaacac cttttttatg agtgtgacac ctagaacggc
    29041 tggatattat tcagttgatt acctccatat gagccatgca ggtctgattt taacgatgtt
    29101 tttgatgtac atcggcggaa catctgggtc gacggccggc ggattgaaaa caactacgtt
    29161 aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga cgtacgagag cggaagcatt
    29221 tggccgtacc atccgacaag gcgcagtgtt gcgggcactt actttatttt ttgttacgtt
    29281 gacgttgtgt gtagtcgcca tcatgatttt atcggtgact gaaacgatac ctaaaacttc
    29341 tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt ggtacagtag gtcttacgat
    29401 gggattgacg ccagacttga ctgcaatagg gaaattgatc attatagcgc ttatgtacat
    29461 tggacgagtt ggtgtattga ctgtggtctt ctctctgatg acaaaagcac aaagaacaca
    29521 atcaaaatac aaatatccag aagaaagtgt catgattgga taatttcatc ttctgacaga
    29581 aacctagtga tttagtaaaa taagaaaata aaataatttt tcttgcttct tggatacttt
    29641 aagtgcaaaa tgttcgttta ttttttgagg cactacccgt tgttgaacct cgggtagtgc
    29701 ctctttttta ttgaaaggaa tcaataagat ttgattcctt tatttatcaa taggttataa
    29761 ttcatatttt tttatttttt cttttacgat acttagtcca gaagctaaaa tttcaaggtt
    29821 attagaggat agggaaattc gaacgaatgc atctggttgc gtacgtgtag taaagcgtct
    29881 ggaatgataa agtcggatac cgttagcaag aaagtcattc tcaattatct tagggtcaat
    29941 ctcaggatca actggaagaa ttcgatagta agggacacga ttcgttggtt tttctacttt
    30001 gaaaagagaa tcaaaaagct gattggctct ttttgtaaga cttaatttag tttctaagat
    30061 cctatttgct tcatttgaat ggaggatttg agtgatgact tccacatcta atccagaagt
    30121 tttcacattt atattaaaaa ttgcttgttc tatcttgctt tggacatgta ctggaactac
    30181 aagataagca actcgcaatc cagaacaaat aaattttgtc attccagcta agtagattgt
    30241 ttggttaggt aataaatttt ggaaaggagg aatgatcgta tgttgatagt aagttgttaa
    30301 aaatgaatgg atatcatctt caataaccca tagaccttct ttttcaatca cttcggctaa
    30361 ttgctctcgt cttttgtgag agatttgaaa ccctatagga ttattacaag aaggcataag
    30421 aaagacacca tgaatcttct tttttctaca ttcctgaatc aatatatcag gtcgcattcc
    30481 ttgctcgtca aaatcaattg gaacgatctc taaatgatat aactgagcca attcaatgaa
    30541 attagcgtat gtatatcggt ctgttgctat tctatcacct ggagagaaaa gtgcagaaag
    30601 tgtgatagcc aatccatttt gaacaccgga aacaatagat agagattcta aggacggaat
    30661 gactccttga gtttttaacc aatttgctgc agtagtcaat tgaaaatttg tgcctttggg
    30721 atcttggtaa ttaagcaaac caacaagatt tgggttatta ctagttgatt gaataaacgg
    30781 gacaacgaat ttattacatt cttcaaaaga actaaccaga ccaaaatcaa tgatatgttc
    30841 ggttacttca ttggtagaga tggtgatgga ctcaaagaca ttgggagaaa taaatgtgcc
    30901 acttccaata ttcgtataca gcaaaccttt ctcaattcca tatttataag ccttgccaac
    30961 ggtagtaaaa tttaaatcta aataatcagc tagctctctt tgggagggta actgagtgtt
    31021 tttctgtaat ttattcttta aaatatcttg ttctaattgt tgaatcaacg atcgataata
    31081 aggacgcttc aatttttgtt tgtctggtcg ccaagttaac gggtaattgt cataagaatt
    31141 aataggcatt ttctccactc cttaaaattg tataccatac aattttacca ttgattgtat
    31201 tgattttaga agttaatctt ttgtgaggag ggaattgtct atgaaaatta ttaattttga
    31261 ggaagtagta gcttctttta gttttgaaga agcgattcaa gtaatgaaaa aatgtttttt
    31321 tgattatgaa agtggtaaaa tcagccaaaa tcctcgattg gtggaaattt tacctgacgg
    31381 aaaaggcaaa aatatgtttg cgatgatgcc tgcttatttg ggggagaaca gatattttgg
    31441 cacaaaagtc attaccgctt ttccggataa tcatcagtat catttacctt ctcatttagg
    31501 agagattttc ttgtttgatg ctcaaaacgg actacccaaa gcattgataa acgcaaatgc
    31561 agtcacctgg attcgaacag ctgctgtatc tgcgctggca acagattact tagcaaatcc
    31621 gcatgcagaa acacttgctt taattggagc tggtcaacaa gcattgtcgc atttgactgc
    31681 gatttctacc attcgtccca tcactacagt gtttgtttac gatattttag aagaaagacg
    31741 aaatactttt atcgctaaag ctaaaaaaca gtatcctaaa ataacgttta taaatgcaat
    31801 atcagtagaa gatgcagtac gtccagcgga aatcatttgt acgttgacct ctagtaaaga
    31861 ggcatttctt caaaaagagt gggtaaggga agacgcacat atcaatgctg tgggcacatt
    31921 tactcctgat acaagagaga taagtacatc gttgatggaa acttctcaaa tctatgtaga
    31981 tgattactca gctgccttaa cagaaagcgg ggacctgctt atagccatgg cagaaggaga
    32041 tttagaggaa tcttgtattg ttggtacatt aggcgaatta gtttctgaga aaaaaacatt
    32101 ttataaagga gaatcgaggc cgacgatttt cgacgcagtt ggtctagcta ttgaagatct
    32161 ttgttgtgca gaatatattt ataacaaatt agagaaaggg gctttaaaat gaacttagat
    32221 tattacgtag ttgatgcatt tgcagatgag gtgttcaaag gaaatccagc agcggtttat
    32281 gtgctagaag aatggctgcc agaaggtacg atgcaaaaaa ttgccattga aaacaattta
    32341 tctgaaacag catttacagt gaagaaaaac caagaatttg agcttagatg gtttacgcca
    32401 gatcgagaaa tcgatctttg tggtcacgcg acattagcaa cagcatttgt tttatttaac
    32461 tattacaaaa tacctgatga aacgattaaa ttttctactc aaagcggtaa tctatttgtg
    32521 acaagaatta aagattatta ttacatggat tttcctagta ttatgccgaa aaaagtgccg
    32581 atattgtctg aatatgaaga agcgattggg gctaagatta aagaagccta cttagcacgt
    32641 gatttatttt tcgtattaga agatgaaaaa acggtggcta aattgcagcc agattttaca
    32701 gctatcaaaa attttgaatt aggaatagga gtcatcgtga cagcagaaag tgtgcaaaaa
    32761 gactttgttt ctagaacctt ctttccaaaa ttaacaatca atgaagatcc agtatgtgga
    32821 tcagcacatt ctaatcttat tccttattgg gcagaaagaa tgaataaaac aaaattgtca
    32881 gcttatcaaa tgtcatcaag gggcggggat ctagaatgtg aattaaaaga agatcgtgtg
    32941 ataataggag gaaaagcagt tttgttttct aaaggtacct cgtttatttg atttctttca
    33001 aatgaattaa aaataaattg gtggagagtg gcaaccttat agctacaatg gttgttaaaa
    33061 aaagacgcaa actttttcaa tttaatgaaa agtttgcgtc tcattttaat tattggtgtt
    33121 tgttttttat tcaatcaaga gtaaaatgcc ccatactaac tatcctcgga tgacttcgat
    33181 tttataacca tcagggtcaa tgacaaagta gtaagaaggc gcagtgccag gtaatccttt
    33241 taagtcagta atctcaagac cagcagcttt atgttgctcg tgtaattttt ctagatcatc
    33301 agctgctata gcgatatgac catagccgtc tcctagatca tacgcaccat gatcatagtt
    33361 gtaagtcaac tctaattcat agtcatcccc aggaagtgtc aaataaacaa gtgtaaattt
    33421 gttttctgga aaatcacggc ggcggctttc ttcaaaacca aatgcctttt gataaaattc
    33481 gattgatgct tcaagatctt tcactcgaac acatgtgtga gccattttca tagattcatc
    33541 tctccttttc cttttactca atcataacaa aaagaagtaa ggaaatagtt gatttgtgct
    33601 tgttcatgag aaatttctca tgtaaaatgg atagaaaata aaactgttaa ggtggaaact
    33661 attcatggat aaaccaattt ttggtgaaaa aaagcaattt gctgattatc aaacccgtta
    33721 tgcagcatat attgtcatag aaagagatca gcagattgca gtaatcgagg ctccaaatgg
    33781 tgcttttttt cttcctggcg gggaaattga aggagaagag acgaaagaag aagcgattgt
    33841 ccgtgagctg atagaagaaa tgggaattgt ggcagaaatt tcttcttatc tgggagaagc
    33901 tgatgagtat ttttactcga attatcgtgc aacttattat tataatccag gatatttctt
    33961 tgttgctgat tcttggaagc gtataggaga accaactgaa aagaccaata aaatttggtg
    34021 ggtgagccct caagaagcgc ttgtgaaatt gaaacgtggt agccaccgat gggcagtcga
    34081 gagatggatc gaaaaaaata aaaactgaaa gaaagcgagt agatcagctt tctctcagtt
    34141 tttttattta ttacagtaca tattttttga ttgcttcagc tacaccgtgc tcgtcgtttg
    34201 atgaagtgat gacatttgct gcttctttga caagagaaac ggcatttccc attgctactc
    34261 ctagaccagc atattcaatc atggataagt cgttctcgtt atctccgata gccatgattt
    34321 catcttgatc aataccaagg tgctgtgcga gattcgcaac tgccgcacct ttacttgctt
    34381 ctttatttag tacttcgaag taatagggcg cacttttgac cgttgtgtat ttatcacgga
    34441 aagtttgcgg taaacgcgca attgctgcat ctaaaatttc tggttcgtcg atcatcatca
    34501 ttttgacgat gttcatgtct ggtgtcattt cttcaggtgt tcgatatttt agcggcatct
    34561 tgactaaact tgcttcgtgg atcgtataag cgctgatatc tcgattaggc gtgtagatcg
    34621 ctttgtcatc gatcgaatgg agatgactgc cgacacggcg tgccatcact tcgatctcta
    34681 ggaaatcttc atgtgtcagt ccgtgacggg cgatgatttt acctgatttc gtttgttgta
    34741 cgagtgcccc gttataagta ataacgtaat cattatcttg aaaaaggttc aactcgttta
    34801 actgttcctg gacacctggt aagggacggc cagtgcataa aacgatcgaa acaccttttg
    34861 caacagcagc ggcaatcgct tcttttactt cttttgttac ttctcttttc tcatttaata
    34921 aagtaccatc gatgtcgata gcgactagtt tgatagacat aatttcctcc aatatatttt
    34981 aatttatttc aatcaatgcg ccattacgga tatggctggc aaattcttga taagtttcgt
    35041 caaataaatc ataatgatca cgcaaggacg gatcaaccat ttcttttgga aagaaaaatc
    35101 gctcatctcc ttgttcttgt cccgctaatg cagcaacaag ctgactaacc tgtgataatt
    35161 ctatcaaagt tccgtcattt cgcaaaatct cgatttgtgt gcgatgtctg ccttgttctg
    35221 ggcgataaaa atcataaggt agatcgtagc tagaattcac agcagtgtaa taaacaggat
    35281 tatatccgac cttttctact aacaaagtga gttcttggac taattttgaa tcccggttgc
    35341 cagaaaaagt agcagatttc agtggtttac gattcaaaaa acgtttagct aaatcattta
    35401 aaataggatc agcagattca gaccattgag taaagtaagt gcccaagacc ccatcatcaa
    35461 gtttgaggta ttcttctaaa gtaaagcttc cgtcaagaaa tggcagaagt aattgagaat
    35521 ggagttcaaa actgctggca tcttcttgat acaactcgtg tgcgcgatga agcagatgct
    35581 ccaaaattac ttccatacca cgggaaacag gatggaagta cacttgcaca tacatttgat
    35641 atcggctaac gatataatct tccactgcat gcatgccact catggaaaaa gcgatcccgc
    35701 cttcataagg acgaatcaca cgtaatattc gggtaagatc aaatgttcca tattctgttc
    35761 cagtgaaata agcatcccga agcaaatagt ccattcgatc tgcatcgatc tgactagaaa
    35821 tcatttggac aacttgtggg ttggggtaag ttttttggat cacactagct accttttcag
    35881 gaaaaccttc ttctactcgg ttaagaatct gatacacttc tgtttctgga gaagtgatga
    35941 tctctacagt gatggcttca tgattcgtat ggaagatatg ttcaaatgta tgagagtaag
    36001 gaccatgacc gacatcatgc aacagtgccg cacataaagt caccagccgt tcctgatcat
    36061 cccagcctcc gttgcctatt ttttcttttg aaaaatttcg agaaaagata tcgcagattc
    36121 gtcttgaaat ctcataaacg cctaatgaat gagtaaaacg gctatgttct gctccgtgaa
    36181 aagtgaaaga agaagtgcct aattgtttaa tgcgtcgtag acgttggact tctttcgaat
    36241 tgatcaaatc taagattact tgatgctgga catggatata gttatggact ggatcacgaa
    36301 aaactttttc cataggcagc atttgatatt tataaggggt tgtcataatg tagctccttt
    36361 atgttattac gttgttccat tctctggatt tggttgttcc attgctccag ctggatttct
    36421 ttttttagcc agtcggtaga atcctgtaaa tcacttgcag aatacagctt ttcaaaagct
    36481 tttgcaaatg cttctttgac ttcttttaca aaaaggggtt ggtcaagcag ttcttcaagt
    36541 gttgccattg tagcaggatc cactgcagga tagccatttg taccaaagtc ttcttttaaa
    36601 gcagattgat aaaatcgctt catgacttgc ccgcgttttt cctgattacc gttgacactt
    36661 aagtacatca taacagaaat cccatttttg atccgacgct gagcgatacc ggcaaatttt
    36721 ttgccatgga tgcttaaatc ataagttcca ggacaataag aatgggtgat ttcgaatgct
    36781 tgtatcggtg cctttggaaa agcgtattta gtcaatagga gcatttgctc gtaagcgtca
    36841 tctatggtta ttttttttgt gtcactttgg gggaaaatca aagaaatatt caaaattcct
    36901 tcatctgata cgatccctaa tccacctgaa tttctgatca ctggagcgta acctgcatct
    36961 ttcagtgttt ggatcccgtc acttagaaaa ggagttcgaa catccttcat acctaagatg
    37021 actgtgcagt catattgcca gaaatgtaaa aagatttgct ggtgttcttt agaatattcc
    37081 gttaatatat ctgttaatgc aaaaggggaa aagtcgtttt tttgataaat gtgctgatct
    37141 agtcgaatcg cagggttcat tcaaccggct cctttgtata aattcatatt ttctacaata
    37201 ttatactcta aaatcaaaga gttgttttgg aaaaaaggtt ttctgtaaaa atggacaagt
    37261 tggttgacaa aacaaaagaa actaggaaca atgtagctat gagaagtaaa ggagaatgaa
    37321 taatggaatt atcacaagga acaccgattt caatcaaatt gcgtacaaaa gtaaaacagc
    37381 aaggggaagt gcaagatttt tattttgatt tgaaagggca aatggtgaca attggcgata
    37441 cgctgtacat acgttataaa gagattcagg aggagacggg cgatgagatt cctgtaacga
    37501 tcaaattagt tccagatggt catgtacagc tgatccgtgc aggagaaatg cggatgcgtt
    37561 tgaaatttgg ctataaagaa cggttagaaa catcctatcg aacaccatac ggtatgatcc
    37621 agattgctac ttttacgaaa gaactgcatg ttagtttgaa agaccgtccc acagcaggga
    37681 aggttcgaat cgattatgat ctgttcatgg gaccggaaaa aatcggcgaa tattatttga
    37741 ccttggattt tacagcgtaa atataaaaga aaagtttgat tttttgtgtc tgattttgta
    37801 tgataaggag tagactgtga aaggacgtgt cctgattgga aattagtgta tttgaaggtg
    37861 caaacaaaag cgaattatca atgatcgaag tagcacacgc gatcttagaa caacgtggcg
    37921 atgtcatgga tttttctgat ttagctaacc agatccaaaa ctatcttggg aaatcagaca
    37981 gtgaaattcg tgattcactc gcacaatttt atactgactt gaatattgac ggcagcttta
    38041 tctcattagg ggataatcgt tggggcttgc gttcatggta tgctatcgat tcgatcgatg
    38101 aagaagtaaa ccatggaatg gacgaagagg atgaagatac tccacgtcgc cgtaaacgca
    38161 agaaagtcaa cgcctttatc aatgatgatg aagacgcgat cgattacaat gacgatgacc
    38221 cagaagatac agatctgaca gaagaagatg acgatgatct gtttgatgac gatgatgatg
    38281 aagatgaaga aatcgctgct tataattctg acttgcaaga aatcggagca gatgacccga
    38341 cagatgatga agaagacttg ccaggaaata tcgaagaaga tttaagtatc atagacgaag
    38401 acgatgatga tgaagattat gaagaggaag aattttctga ctctgaaaaa gaataaacat
    38461 tattttctta cgagaggttg tgacagagac gatcagccta tcagaataag gaggtgtgac
    38521 acgatttttg tcacaccttc ttttttgtat ataaaaatcc caagtaaata cataaaaaat
    38581 aaaaaaattg aaacttttag cgcaccttgt acgtcatatt aagtgaggca gaaaggagtg
    38641 gagaatgatt tttactggat ataagaaaaa acggcagatc gaaaaatacc gtcacctgct
    38701 gaatggactc atcgaaactg attatcaaaa aatgttccgt atcacgatga attacgtaca
    38761 tgataaagaa gaggccttag atgtgatgca agatagtttt cataaagccc tgaactattt
    38821 tgaaaaagaa aaagagatcg agcattttag tgcatggttt tatcggatat tgatccgcac
    38881 ggcattagat agttggcgca gacaaaaaag gacgcctgtg gacttatttg aaattcaaga
    38941 tatcccccaa cagttacctt tagaaacttc cgttgcagag ctccatatga ttttagataa
    39001 aatagacagt ccggaaaaag agattctgat cctctacttt tttgaaggat ttcgtttgaa
    39061 agaaattgcg actatcttgg atatgaatga aaacacagtt aaaacgaaaa tgtaccgctc
    39121 attgaatgct ttacgtcaaa tcctagaatg aaggagagaa gagtgccatg tattcccaaa
    39181 aagaaaattt aaaaaaacta aagaaaactt ataaagaaca agcaattccc aaagggctta
    39241 aagaggagat ccgccagcgt tttattatcg aagagcaatc atttataaaa gcaaagcgga
    39301 gaaagagacg tctccaaggg ttcggagtat ctttggctat cgtaatgatt acaacaagta
    39361 gccttctttt taatagtcaa gttcgaagct ttgcagaaga tttacccatc ttaggatcag
    39421 tcattgaatt gatcttaggc gaacgcttta ctgatcgatc tgaaaaaatc gatattcagg
    39481 taccgaagat caatacacaa aatgagaaag aaaataaaac aattcatgga ttaaatcaaa
    39541 aatactttcg cgaaggacaa gcagattttg aaaaagctga aaaagaatat ggaaattttg
    39601 aaacggatca ttaccaagtt ttgggagatt atcagaaaat cgtagacgat aaccggtttc
    39661 tcgtgattga aaggcagatc acccaaacag cagcagatag ccatgttgaa aaaagatacg
    39721 acacaatcga caagaaaaac agtgtccagc tctcacttcc acttttattc aaagatgaca
    39781 cctatctatc tgttttgaca aaagaagtca aacggcagat ggccgaacaa gtcaaggaag
    39841 atccatctaa gtactattgg actgagcaag atattcaaga agggacgata gaaaaaccaa
    39901 cacttgttac gcctacacga agtttttatt tgaacaaaga gcatcaatta gttttgactt
    39961 tttctcaata cgaaattgct cctggttata tgggtacacc agaatttgtc attcctaagt
    40021 ccgtgacaaa aacgattttg gcatcggaag attatttgga tcattagacg agaagtagtt
    40081 gcgactagca gtttcaattt tcaagtaagt acaagtaaat aataagaaga gaaaaagaga
    40141 aagaagagat atagtgaaaa gaaattattt agttatcagt attattgctt tatccgtgct
    40201 tttgtttttc gaaattactt cggtggcctc cttgatcacc gaagtaaaag gacaaacaca
    40261 ctcttcggag cattcgtcac aatctttcaa acaatcagaa caatcaacca ttaaaagcca
    40321 atcaactgaa acagctaacg tcaaagaagc cattcaagaa tcagcggaaa aggtacaaag
    40381 tcagtcatca caatcaacac aagacagtac aaaaacgagc cgagatccgg ctgtcaaaag
    40441 cgtcgcaatc agctttgatg atggaccagg agcgaccaca acaccgcagc ttctgcgaat
    40501 cttaaaagaa aaaaacgtgc atgccacttt tttcgtctta ggagaaaata cggcgcagca
    40561 tcctgaaatc gtgaagcaga cagctgaagc agggcacgag attggcaatc acacatacga
    40621 tcatcagaat ctggccactc tttctgccca gtcgatgacg gaggaagtga cgaaagcaga
    40681 cactgagatc aaaaaagtca ctggcaaaac ccccactttt gttcgtccgc cctatggttc
    40741 tgtcacgaat gtaggagcaa cgatcatcca gcgaccgatc atcgaatggt ctgttgattc
    40801 agaagattgg aagacaagaa atcctgattt gattttgcag aagatccaag caacagtata
    40861 cgatggggca attattttat tccatgatat ctatcctgaa acgattcgtg ccgttcctca
    40921 agtgatcgat tatctgaaag agcaagggta tcgcatcacg acagttgggg atttattagg
    40981 gcatccaacg gctgtcgaga actattatgg cagaaacgat catcggccag ttcagtaagt
    41041 gagacaagta tctttaataa ataacacatt ttaataagta gttgaacaca taactacctt
    41101 acttgcaaag gcttaacaat atctatacag gtacaacaaa aaactgtcca ccaattttct
    41161 ttggcggaca gttttcactt aaaaataaag aagcaactat tcgtaaatta tgctaaaata
    41221 gcagtagaac gcgtcctgag ggaatcgaac ccccgtctca agaaccggaa tcttacgtga
    41281 tatccactac actaaggacg caagcactgg gataattgta actaaacaga taaaaaatta
    41341 caaggggatt ggacaaaaaa atgaaatttg agcaaaattt ttcacaaaaa cagcagcaga
    41401 cacagaaact agccatgacg caacagttgc aacagtccat ccaagtttta caatataaca
    41461 gtgaggagct gttggcattc gttgagaatc aagcaatgga aaatccattg gtggaagttg
    41521 ttgagccgga atggcagcca gattacataa aagcttcttc atcttcttat gaaggagaag
    41581 agacaaatta cctaaatcag attcctgata caaaagggtc tttatttgat tcattgatcg
    41641 aacaagtcca tttgaattac cgagatactt ttttgaggaa aatcgttctt tatcttgtag
    41701 aatacatcga tttgaacgga tttttgacga ttagtttaga agaagccgga aaagagattg
    41761 gagctacgcc tatccaaatg ctcgatgcgt tgactctgat tcagcaatta gaaccagctg
    41821 gagtaggtgc acgtagtttg caagaatgtc tcatgcttca aacagaacga gatgattatg
    41881 cgccggaatt agcttatatc gtgttggaag aatgttttga ggaactggtg gagcgaaaat
    41941 ggaaagaaat cgcacaacgt ttttctgttg atctacatgc tgttcagcag atattcgact
    42001 atttacaaac gttgagtcca agccctggcc ggattttcga ccgtagcagc gaattgtaca
    42061 ttattcctga tgtccgggta ttagtggacg acgataaaaa tgtacaagtt atctcaaacc
    42121 gaaaaaatca gccaaatata cgctttcaag agagttactt caaacaaatg tcacaacaag
    42181 cagataaaga aactgaaaat tatttaaaag aacggaaaca ggaattcgaa tggttgaaga
    42241 aaacgatcct acaacgtggg gatacaattt tgcgggttgc tcaagtcatt gtttcccggc
    42301 aaaaagcttt ctttatcgac aaggaacgtc caatcaagcc tcttacgtta aaggaagttg
    42361 caactgagat agatgtgcat gaatccacgg tcagtcgtgc agtcaacggg aaatatttgg
    42421 aaacagactt cggtgtattt gaattaaaaa aatttttcac tacacgaatt cctacgaata
    42481 gtacagaaca gacagaagac ctttcagcag atacagcgaa gaaaaagctg caggaattag
    42541 tcgatcaaga agacaaaaac aagccgttgt ctgaccagaa attggttgaa ttgctgaaaa
    42601 aagatgagat agcgatctca agacgaacag tagcaaaata tcgtgattta ttaggaatcc
    42661 cgagttcatc aaaaagaaaa cgttatgata actaatcagt caaaagacta gaaaaagtat
    42721 ttcacttttt ctagtctttt tagttgaaaa acgagagcaa aactgttaga cttacagtcg
    42781 tgaggttgag atttcccttt attgatgtga aattcgaata gttaatttct ttttttatcg
    42841 tatatgggtc gttttaagtc tacattggac gtaaagtgtc caactaagga gatcgctatg
    42901 ctggatgaaa taaaaatgat tgaatctgtt gcaccagata ttatcgaagt gttgcaagaa
    42961 cgctataaaa ttttacgaaa tatctattgg atgcagccga ttggacgtcg aagtttatcg
    43021 gaaagtatgg ggatcacaga acgagtattg cgaacagaaa cagatctttt gaaaaatctg
    43081 aatttgatcg acacctcaaa aagcggtatg acgctgacaa ctaagggcga agaagtttat
    43141 caaagccttg aaaacttcat ggatcagctt ttaggtacgc atcaaaccga acaacagttg
    43201 gcagaatatt ttggtaacca acgctgtatc gttgtttcag gaaacagcga agagcagacc
    43261 aaagtggctg atgcattcgg cgaagcgctg agcgaagccc tagaccgact acttccagaa
    43321 ggcgagaata tcatcgctgt gatgggagga actactatgg ctgtggtagc cgaacaattg
    43381 tctaatctgg aaaataagaa acggcataac ctgtttgtcc ctgcaagagg cgggattggt
    43441 gaagcgataa ctgtacaggc aaattctgtc agtgcaagaa tggctgctaa agcaaacggt
    43501 aaccaccgtg cactttatgt tccggaacaa ttaagtctgg caacttacaa ttctttactc
    43561 aatgagccat ctatccaaga agtcttaaac ttgattagtg aagcgaattg tgttattcat
    43621 agtattgggc gtgcattaca catggcagca cgtcgtaaga tgactgaaaa agaattagtc
    43681 atgctgaaac aagcaaatgc tgttgcggaa tcttttggct actttttcaa tgaaaaaggt
    43741 gaagtcgttt ataaggtccc tagaatcggc atagaattgc aagatttgga aaaagtcccg
    43801 atcatcatgg caatcgcggg cggaaaatca aaagctaaag caattcgagc ttatatgaaa
    43861 aatgcaccaa aacaaacgtg gctcatcact gatgaagctg ctgcaaatga gattttaaaa
    43921 ggggtaaccc tttaaaataa atattttttt gattttcata aggaggaaat cttaaatgac
    43981 agttaaagta ggtattaatg gttttggacg tattggacgt ctagcattcc gtcgtatcca
    44041 agatgtagat ggaatcgaag tagtagcaat caacgacttg acagatgcta aaatgttggc
    44101 acacttgtta aaatacgaca caactcaagg acgtttcaac ggaacagttg aagttcatga
    44161 aggttcattc aacgtaaacg gaaaagaagt taaagtatta gctaaccgca acccagaaga
    44221 attaccatgg ggcgaactag gcgtagatat cgttctagaa tgtactggtt tcttcacttc
    44281 taaatcagca gctgaaaaac atttaacagc tggtgctaaa cgcgttgtta tctctgctcc
    44341 tggcggaaac gatgttccaa caatcgttta taacacaaac cacgaaacat taactggtaa
    44401 agaaacagtt atctcaggtg cttcatgtac tactaactgt ttagctccta tggctaaaac
    44461 attgaacgac aaatttggtg ttgttgaagg attaatgaca actatccacg cttacacagg
    44521 tgaccaaatg actctagacg gacctcatcc taaaggtgac ttccgccgtg cacgcgctgc
    44581 tgctgcaaac atcgttccta actcaacagg tgctgctaaa gctatcggtt tagtaattcc
    44641 tgaattgaac ggtaaattag acggagctgc tcaacgtgtt cctgtaccaa caggttcatt
    44701 aacagaatta gtaacagttc ttgaaaaaga agtaactgtt gacgaaatca atgcagcaat
    44761 gaaagaagct tcaaacgaat cttacggata caacacagac gaaatcgttt cttcagatat
    44821 cgttggtatg acttacggtt cattatttga tgctacacaa actaaagtaa tgacagttgg
    44881 cgacaaacaa ttagttaaaa ctgttgcttg gtacgacaac gaaatgtcat acactgcaca
    44941 attagtacgt actttagaat acttcgctaa cttataagat agacaaacgt acaagtgtaa
    45001 attatagaca tgtacattta aaagcgggga agcaatcgcg cttcttccgc tttttttgat
    45061 aataagttta ttcaggagga aataaaatgg ctaagaaaac agtgaaagat atcgatttaa
    45121 aagacaaaaa agttcttgtc cgtgttgact tcaacgttcc tttgaaagac ggcgtgatca
    45181 ctgacgacac tcgtatcaaa gcagcattgc caacaatcaa ctacgttcta gaacaaggcg
    45241 gaaaagcaat ccttttctct caccttggac gtgtaaaaac agaagaagat aaagaaggaa
    45301 aatctttagc tccagttgca aaacgtctag gcgaattatt aggtaaagaa gtaacattcg
    45361 tacctgaaac tcgcggagaa caattagaag aagcaatccg caacatgaat gacggcgacg
    45421 ttgtcgtatt cgaaaataca cgatttgaag atattgacgg taaaaaagaa agcaaaaatg
    45481 atacagaact aggtaaatac tgggcttcat taggcgatgt atttgttaac gatgctttcg
    45541 gtacagctca ccgtgcacat gcttctaacg taggtatcgc ttcaactggt atcccaacag
    45601 ttgctggatt cttgatggaa aaagaaatca agttcatcgg tgaagcagta gaagaaccaa
    45661 aacgaccaat gattgcaatc cttggtggcg caaaagtttc tgataaaatc ggcgtaatcg
    45721 aaaacttgat tcctaaagca gataaaatct tgatcggtgg cggaatgact tacacattct
    45781 acgaagcaaa aggaatcaag atcggtaact ctctagtaga agctgataaa gtggaactag
    45841 caaaagaatt aatcgaaaaa gcaggcgaca aacttgtctt gccaatcgac aatgtctgtg
    45901 caccagaatt ttcaaatgat gttgaaacac aagtaatcga aggagacatt cctgatggtt
    45961 tgatggcttt agacatcggc ccagcatcag taaaattatt tgctgataca ttacaaggtg
    46021 caaaaacagt tgtttggaac ggacctatgg gtgtatttga aatgagtaac tttgctaaag
    46081 gtacaatcgg tgtttgtgaa gcaatcgcta acttagaagg cgcaacaaca atcatcggtg
    46141 gcggagactc agccgcagca gcagaacaat taggatttgc tgacaaattc acacacatct
    46201 caacaggtgg cggcgcaagc ttggaattgc ttgaaggtaa agaattacca ggactagcag
    46261 caatcaacga taaataaacg accaagtttg ctcaattctg acgagtaatc gaacaaaagg
    46321 tgaagggagt tttcatgact ccctagccat ttttcaataa attaaggagt gtgttcctca
    46381 atgcgtaaac ctattatcgc aggtaactgg aaaatgaaca aaactgcatc agaagcaaaa
    46441 gaatttgctg aagcagtaaa aaacaaaatc ccttcaaatg acaaagttga ttcagtcatc
    46501 ggatctcctg cattattctt acaagaatta atgacatcag ctgaaggtac agaactaaaa
    46561 atctcagcac aaaactgcta ctgggaaaac tcaggtgcgt tcactggtga aacttcacca
    46621 gcagctcttg ctgatctagg tgttcattat gtgatcatcg gacattcaga acgtcgtgaa
    46681 tatttccatg aaacagacga agacatcaat aaaaaagcga aagctatctt tgcaaatggt
    46741 atgacaccaa tcctatgctg tggcgaatct ttagaaacct atgaagcagg caaaactgct
    46801 gaatggatcg aaggacaaat tacagctggt ttagctgatc tttcagctga acaggtgtca
    46861 aacatggtta ttgcttatga accaatctgg gcaatcggta ctggtaaatc agctgacgca
    46921 caaatcgctg acgaaatttg tggagttgta cgtcaaacag ttgaaaaact ttatggaaaa
    46981 gaagtatcag aagcagttcg tatccaatac ggcggttctg taaaaccaga aaatattgct
    47041 gaatacatgg caaaagaaaa tgttgacggt gctttagtcg gtggcgcaag cttgcaagca
    47101 gattcattct tgtcattgtt agacgctgta aaataattgt tgaaaatttg cgaaaaatgc
    47161 aagaaatgtt tgcactaatg agcaaaatta actaaaatcg tatatgaggg aaatccctta
    47221 ggaaaaaaca aactcaaagg agagacaaaa catgtcaatt attactgatg tttacgctcg
    47281 cgagatctta gactcacgtg gtaacccaac aatcgaagta gaagtataca cagaaagcgg
    47341 agcttttggc cgcggaatgg ttccttcagg tgcttcaact ggtgaatacg aagcagttga
    47401 attacgtgac ggagacaaag ctcgttatgg cggtaaaggt gtaactaaag ctgtcgacaa
    47461 cgtaaacaac atcattgctg aagcaatcat tggctacgat gttcgtgacc aaatggctat
    47521 cgacaaagct atgatcgctt tagacggaac tcctaacaaa ggtaaattag gtgcgaacgc
    47581 tattttaggt gtttctatcg ctgttgcacg tgcagctgct gactacctag aagtaccttt
    47641 ataccactac ctaggcggat tcaacacaaa agtattgcca acaccaatga tgaacatcat
    47701 caacggcgga tcacatgctg ataactcaat cgacttccaa gaattcatga tcatgcctgt
    47761 tggcgctcca acattcaaag aagctctacg tatgggtgct gaagtattcc acgcattagc
    47821 ttcaatcttg aaagctcgcg gattagcaac ttctgttggt gacgaaggtg gatttgctcc
    47881 aaaccttggt tcaaacgaag aaggttttga agtaatcatc gaagcaatcg aaaaagctgg
    47941 ctatgtacct ggtaaagacg ttgttcttgc tatggatgct gcttcttcag aattctacga
    48001 caaagaaaaa ggtgtttacg tactagctga ttcaggcgaa ggcgaaaaaa caactgacga
    48061 aatgatcaaa ttctacgaag aattagtttc taaatatcca atcatctcaa tcgaagacgg
    48121 attagacgaa aacgactggg acggattcaa gaaattaact gacgtattag gcgacaaagt
    48181 tcaattagtt ggtgacgact tgttcgtaac aaacactcaa aaattatctg aaggtatcga
    48241 aaaaggaatt gctaactcaa tccttatcaa agtgaaccaa atcggtacat taacagaaac
    48301 tttcgaagct atcgaaatgg ctaaagaagc tggctacaca gcagttgtat ctcaccgttc
    48361 tggtgaaaca gaagattcaa caatctctga tatcgctgtt gcaacaaacg ctggccaaat
    48421 caaaactggt tctctatcac gtactgaccg tattgctaaa tacaaccaat tactacgtat
    48481 cgaagaccaa cttggtgaag ttgcagaata caaaggtttg aaatctttct acaacttaaa
    48541 aaacaaataa ttcttttcta atgaagatac atgatcgact attgaaagat agtcgagtat
    48601 gatcacttag ttaagtaatc gaactctcta cttgctgaaa tatcagcagg tagagagttt
    48661 ttttgcaact agcttctgta tatgatgttt acaaagaatg gtacaagaaa agatcatcct
    48721 tgaaaaagga aagctaacaa taactagcat catataagag cttttaagga tagaacagta
    48781 gaataggaat ctatgagcaa gtcctctcgt ataaataaaa taggagggaa gtagcagcat
    48841 atttttcata aagtggctct ctttcttcat ttttactttg gttgctccta tttgaaaatt
    48901 cgagcacact caaatacaat aaaaagaaat ggcaagggag aggattagat gaaaaaagaa
    48961 gaaaaaatcg cgattttaca agagatcatc cgaattaaat cagtgaatgg aaacgaaggt
    49021 gaagtagcag cctatttgaa taagcttttg gcaaagcatg acataacagg agagattgtt
    49081 tcttacagag atggtagaga taatttgatt gctcgttacc aaaagggcca atcaggaaaa
    49141 gtgttaggcc tatctgggca tatggatgtc gtggcggctg gtgatgaatc gtcttggacc
    49201 tatgcaccct ttgcagcaga aatccatggg aaccgtttgt acggacgtgg tgctacagat
    49261 atgaagagtg gcttggcagc aatggtgatt gccatgatcg aactgaaaga gtcaggaaaa
    49321 ccatttaacg gaacagtcaa attattagcc actgtcggag aagaagtggg tgaactaggt
    49381 ggggaacagc tcacgaaagc aggctatgta gatgacttag atgccttgat catcggagag
    49441 ccaaccaatt acagtttgat gtatacgcac atgggatcaa tcaactatac agtcacctcg
    49501 cacggaaaag aagcgcatag ctctatgcca gaccaaggtt ataatgcaat caatcaccta
    49561 aatgaattca ttacgaaagc aaatgctgaa atgaatcacc tcgctgaaac aatcgagaat
    49621 cctgtcctag gaaaaacaat ccataatgtt acattgatca gtggcgggaa tcaagtcaac
    49681 agcatcccaa gccatgcgca gctccaaggg aacattcgat cgattccaga atacccaaat
    49741 gataaaatca ttgcattgct gcaatcaatc gtcaatgaat tgaatcaaga gacagactat
    49801 catttggaat tgatgatcga ttacaacaaa atacctgtca aagctgatcc agattctcct
    49861 ttgattcatt gcattcagca acaattcagc cagccattac ctttagttgg agcggcagca
    49921 acaacggatg cagccgaatt tacgaaagct aaccattcct tcgactttgt cgtctttggg
    49981 cctggtgtcg ttactttgcc tcatcaagta gatgagtatg ttgaaatcga taattatttg
    50041 gatatgatcg aaaaatatca aggaattatt ttatcctact tagcgtaaga tttttttaat
    50101 aaatgaaaaa acagagccag attagcatta
//
LOCUS       DAZULG010000009        50009 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000009 DAZULG010000000
VERSION     DAZULG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.009"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..1722)
                     /locus_tag="ACG9CY_000432"
     CDS             complement(22..1722)
                     /locus_tag="ACG9CY_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936972.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2229..3389
                     /locus_tag="ACG9CY_000433"
     CDS             2229..3389
                     /locus_tag="ACG9CY_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4936973.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3405..3848
                     /locus_tag="ACG9CY_000434"
     CDS             3405..3848
                     /locus_tag="ACG9CY_000434"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4936974.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4331..4861)
                     /locus_tag="ACG9CY_000435"
     CDS             complement(4331..4861)
                     /locus_tag="ACG9CY_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS4936975.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            4966..5517
                     /locus_tag="ACG9CY_000436"
     CDS             4966..5517
                     /locus_tag="ACG9CY_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4936976.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5670..6143)
                     /locus_tag="ACG9CY_000437"
     CDS             complement(5670..6143)
                     /locus_tag="ACG9CY_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4936977.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6281..7753
                     /locus_tag="ACG9CY_000438"
     CDS             6281..7753
                     /locus_tag="ACG9CY_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4936978.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7750..8460
                     /locus_tag="ACG9CY_000439"
     CDS             7750..8460
                     /locus_tag="ACG9CY_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS4936979.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            8812..9096
                     /locus_tag="ACG9CY_000440"
     CDS             8812..9096
                     /locus_tag="ACG9CY_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS4936980.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9413..9796)
                     /locus_tag="ACG9CY_000441"
     CDS             complement(9413..9796)
                     /locus_tag="ACG9CY_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS4936981.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(9798..10382)
                     /locus_tag="ACG9CY_000442"
     CDS             complement(9798..10382)
                     /locus_tag="ACG9CY_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS4936982.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(10429..11904)
                     /locus_tag="ACG9CY_000443"
     CDS             complement(10429..11904)
                     /locus_tag="ACG9CY_000443"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS4936983.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12085..12396
                     /locus_tag="ACG9CY_000444"
     CDS             12085..12396
                     /locus_tag="ACG9CY_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4936984.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12511..14847)
                     /locus_tag="ACG9CY_000445"
     CDS             complement(12511..14847)
                     /locus_tag="ACG9CY_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS4936985.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            14989..15657
                     /locus_tag="ACG9CY_000446"
     CDS             14989..15657
                     /locus_tag="ACG9CY_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4936986.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15660..16703
                     /locus_tag="ACG9CY_000447"
     CDS             15660..16703
                     /locus_tag="ACG9CY_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4936987.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(16940..17821)
                     /locus_tag="ACG9CY_000448"
     CDS             complement(16940..17821)
                     /locus_tag="ACG9CY_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4936988.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            17980..18708
                     /locus_tag="ACG9CY_000449"
     CDS             17980..18708
                     /locus_tag="ACG9CY_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS4936989.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            18864..19181
                     /locus_tag="ACG9CY_000450"
     CDS             18864..19181
                     /locus_tag="ACG9CY_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4936990.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            19174..19782
                     /locus_tag="ACG9CY_000451"
     CDS             19174..19782
                     /locus_tag="ACG9CY_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS4936991.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            19837..20628
                     /locus_tag="ACG9CY_000452"
     CDS             19837..20628
                     /locus_tag="ACG9CY_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS4936992.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            20766..21254
                     /locus_tag="ACG9CY_000453"
     CDS             20766..21254
                     /locus_tag="ACG9CY_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4936993.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            21305..22177
                     /locus_tag="ACG9CY_000454"
     CDS             21305..22177
                     /locus_tag="ACG9CY_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4936994.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            22353..22715
                     /locus_tag="ACG9CY_000455"
     CDS             22353..22715
                     /locus_tag="ACG9CY_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4936995.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            22724..23476
                     /locus_tag="ACG9CY_000456"
     CDS             22724..23476
                     /locus_tag="ACG9CY_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4936996.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            23490..25292
                     /locus_tag="ACG9CY_000457"
     CDS             23490..25292
                     /locus_tag="ACG9CY_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4936997.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            25569..26609
                     /locus_tag="ACG9CY_000458"
     CDS             25569..26609
                     /locus_tag="ACG9CY_000458"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4936998.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26661..28805)
                     /locus_tag="ACG9CY_000459"
     CDS             complement(26661..28805)
                     /locus_tag="ACG9CY_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4936999.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(29162..30535)
                     /locus_tag="ACG9CY_000460"
     CDS             complement(29162..30535)
                     /locus_tag="ACG9CY_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4937000.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(30535..30930)
                     /gene="rbsD"
                     /locus_tag="ACG9CY_000461"
     CDS             complement(30535..30930)
                     /gene="rbsD"
                     /locus_tag="ACG9CY_000461"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS4937001.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(30958..31875)
                     /gene="rbsK"
                     /locus_tag="ACG9CY_000462"
     CDS             complement(30958..31875)
                     /gene="rbsK"
                     /locus_tag="ACG9CY_000462"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS4937002.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(31983..32996)
                     /locus_tag="ACG9CY_000463"
     CDS             complement(31983..32996)
                     /locus_tag="ACG9CY_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS4937003.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(33077..33676)
                     /locus_tag="ACG9CY_000464"
     CDS             complement(33077..33676)
                     /locus_tag="ACG9CY_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4937004.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            33862..34563
                     /locus_tag="ACG9CY_000465"
     CDS             33862..34563
                     /locus_tag="ACG9CY_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937005.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            34573..37284
                     /locus_tag="ACG9CY_000466"
     CDS             34573..37284
                     /locus_tag="ACG9CY_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS4937006.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            37735..39363
                     /gene="treC"
                     /locus_tag="ACG9CY_000467"
     CDS             37735..39363
                     /gene="treC"
                     /locus_tag="ACG9CY_000467"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS4937007.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(39423..40604)
                     /locus_tag="ACG9CY_000468"
     CDS             complement(39423..40604)
                     /locus_tag="ACG9CY_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4937008.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            40856..42379
                     /locus_tag="ACG9CY_000469"
     CDS             40856..42379
                     /locus_tag="ACG9CY_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4937009.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(42448..43176)
                     /locus_tag="ACG9CY_000470"
     CDS             complement(42448..43176)
                     /locus_tag="ACG9CY_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4937010.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(43189..44469)
                     /locus_tag="ACG9CY_000471"
     CDS             complement(43189..44469)
                     /locus_tag="ACG9CY_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4937011.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(44610..45212)
                     /locus_tag="ACG9CY_000472"
     CDS             complement(44610..45212)
                     /locus_tag="ACG9CY_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4937012.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            45457..45663
                     /locus_tag="ACG9CY_000473"
     CDS             45457..45663
                     /locus_tag="ACG9CY_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937013.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            45975..46694
                     /locus_tag="ACG9CY_000474"
     CDS             45975..46694
                     /locus_tag="ACG9CY_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4937014.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(46775..47458)
                     /locus_tag="ACG9CY_000475"
     CDS             complement(46775..47458)
                     /locus_tag="ACG9CY_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS4937015.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            47677..49863
                     /locus_tag="ACG9CY_000476"
     CDS             47677..49863
                     /locus_tag="ACG9CY_000476"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS4937016.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        16264 a         8849 c         9980 g        14916 t
ORIGIN      
        1 ggatgtattt atcccttttg cctaagcacg taaaagcttg tatctatgat aaataaataa
       61 acatagcaag gtaaaccagc caatcaaagt aagagaaaga atgaaataga aatagattgg
      121 cgtttgataa tgaagcgtga gctcgttgat tcctttttgc tgcttcacga ttggggtccc
      181 gatatctgtt acttcataat cagtcaatgt ttttccattt tgctgtagca cagtccgatc
      241 gtagacgata acagggatat gaacagtttt gtccccagtg tttttccact tgattgtcaa
      301 aagaccgcct ggatgtgttt ttataaaagt tgtttcgttt tctagtattt cttcgttata
      361 ccggtcgtat ttattgtctt ttgtctcctt atagattggc agatagtcag gtgtggtttt
      421 ttggaaaacg aacaagctgg tagacatatc tttgctgaag aatgttttcc ttgcttcttc
      481 cggcgtatcg aatagataag tatgtcgact gacaaatgtt tcattttccc acttttccag
      541 atgctggtat aagttctgga tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa
      601 aagacggaac cacttttgtt cagaccactg gttcagtgaa agggcaaggt aaagtagaag
      661 caatagcgta aatggcacga aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac
      721 atttttttcg atcaatgtcg tccatggaat caagctagta gaaagaaaaa agaaaacaag
      781 acttgtgtac aacacgattt tttggaataa ggtgttcttt tgttgaaaga aaataaaaag
      841 caaaaaacct gataatatca atataggaaa ggccttaggg taatggagcc aataactgcc
      901 atccagatct atcgcctttt gactcatttc tctgttaaca aagggaggaa gaatcttatt
      961 cccactgtaa acaatagcta atcctgccca aatatttgct gtcagaacaa aataaagcaa
     1021 gaccgactta atcagttgaa gcagtgcttt tcctttttca gtctggttaa aaaagtaata
     1081 aagataaaaa gggatataca tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt
     1141 cataagtccc actgagacag cgactttgat aacagggaac tttttatgaa ggacgaaatc
     1201 agcaataggg atcatacata aaggataaaa acaagtcccc cagctagtga atccttggtt
     1261 gatagaccag tattgaatag aatacgtact catgaataaa acggctactg gtacacttac
     1321 ccaatttcga acattgacat atctcagtaa aatcagcata gaaaatcctg aaataagata
     1381 aagaatgaaa tttgaaagaa tctgataatg aaaccagcta cctgcaagca acacgatcaa
     1441 tccttgtaaa taagcgaaaa atgggccgta aacggcattc acgatcctgc cactttgctg
     1501 gaatccataa agggaaggaa aataatggaa atccccatat ttaatttgtt gcgcggcatc
     1561 atagaaccga ttgtaatgga atggggcatc agcacccaaa attagtttat gtgtgaataa
     1621 atgaggcatg agaaatgcca aagcaatgaa aaaaattgct aaaaatgggc catatttttt
     1681 caattgtcga actcctttgc aaactttaga taaattcatc atatatccta tgcgagaggt
     1741 aggcaagaaa ttagaaaatt catgaaaata tttatgaatt ttacaagaaa aacataagtg
     1801 aaagttctaa ggagagagga tgcgacataa gttttgttgc atctccttgt tttgaaacag
     1861 aatttttctt aatactcgaa acaaagtagc tttttcatga cctctcggta aacggtgttc
     1921 aatcagctgc ttctcggtaa taagctcact agcctagaaa atatgagaag ctattttctg
     1981 actagcgctc ttattactca aagcataacg cagttttcac aacctcttcc ttaaacggtg
     2041 ttcaaaagct tgcatctgcg gtaataagcc caacccaaca aaaaatatga gtagctattt
     2101 ttttgggctg tacttattac ttgatgcagg acagcttttt cacaacctca tcggctttca
     2161 ttttctattt ttttcctgaa cccatataat aaaagaaaga acaaaaaaac ttgcaaggga
     2221 ggacacatgt gaagcagagt aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt
     2281 tattaatatg tatcgtcgta gggttactga tcgctttata tccattttat gtggattctt
     2341 taaatagttt tattgatcaa aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa
     2401 ttgaagtgca gcgaaagaaa atggaggaga aaaaccaacg attaactgac caaggattca
     2461 atcctggtac tgatcctttc aatgaaaaga acagaaatga aagtacaaca tccagccaat
     2521 tagaggaatg gctgatcgga agtatcaata tacctaagat ccaattaaat atttcacttt
     2581 atgatcggct caatggtagg atccttgaaa atggagcagg cgtcttgcaa ggaacctctt
     2641 tcccgcttgg tggaaattcg acgcattcag tgatctctgc tcacagcggt ttacctaatc
     2701 gacggttatt taccgaatta gatcgtttag aaaacggaga cacgtttatt ttgactgttt
     2761 tgggggagaa attggcttat caagttaaaa atatacaagt ggtactgcca gatgacacgt
     2821 ctgttctaac gatttcagaa ggagaagact tagttacctt gcttacatgt acgccgtata
     2881 tgatcaacac gcatcgtttg ctagtgacag gacatcggat tccttataac gaatcggtaa
     2941 aaaaagaaga aggaaaagga aatcaggaaa gaaccatgcg gcaatggttt attcttgcag
     3001 gaacgataat agctgtagtc atactgttag tgtttatcgg acgactaatc tatcagtata
     3061 gattaagtaa aaaagtgctt gatttttcat ttattattag tgattcgtca gggaaaccgg
     3121 taaatggtag ttcattcatt ttgaaacata agaaaaaaac acttactaga aatggtgtgc
     3181 cgttttcagt acaaagcgac catcatggga aagtcaagct ggatcaattg cctggtggaa
     3241 cttatcgaat cgtttcagct gatgatccta aagttgctgt ttcttttggt attagaaaag
     3301 taaaacaaga gaagatatac ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa
     3361 atggttttta ttttaaaatc aatgaataag taaggagaga aaaaatgatc cgcacaacaa
     3421 caaaattgtc tgccaaagaa ctggaatctg ttctatcgat ttggctgcat gctaacttgg
     3481 attctcatga attcattcca tcaacttatt gggagaatca agtagaatct gttaggaaag
     3541 ctttaccaga tgcagtgatt tacttagcag aaatagatca agaaattatt ggttttgctg
     3601 gggtgattga tcagtttatc gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa
     3661 tcggtacgca actaatgaaa aaaataaaag aaaccaataa aacactgact ttgacagtct
     3721 atcaaaaaaa tcaatcagct attcatttct atctttcaca aggttttaca attcaaacac
     3781 agcaaaaaga tgaagcagta aatgaggagg aacttgtgat gaaatggaat caaaacgctg
     3841 atttttgatg aacagaataa cgaaataatg ctcccagcct aattttttgc tgggagcatt
     3901 atttcatttt gaagtcacta atttaactgt gtgaatatgc agttgttaca atctttgata
     3961 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
     4021 tatttttcca tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttgat
     4081 aaacggtgtt caaacggctg cttctcggta ataagcccac tagcctagaa aatatgaaaa
     4141 gctattttct aactagcgtt cttattactc aaagcataac gctgatttca caacctcttg
     4201 ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaacatgagg
     4261 aactattttt ccgtttcttt ctctaatact caggtcagaa cgctgatttc acaacctctc
     4321 tttcacaacc ttattttact cgtttataat tttcatcgaa gtattttccg ctacggatat
     4381 catctggcat tccatcgtat ggttcttcgc taataacatc ttcattgtct atacctgcat
     4441 cacccatata agtgatcgta gcaaattcag gaacaagtaa ttttggataa gtttcatatt
     4501 tttcacgagc tgcttccatt tctgcgatat gttcgttttg gaaagtaaca gtgatttctg
     4561 gatgttcttc aacccattta gggaaaaaga tcattccgtg aagtttcttt tcatttggca
     4621 taaagtccaa tgccacgtct gtccctgttg gctctgtcca agtgattttg aatacgccgt
     4681 ccgtcaattt aacgatatca gcttcttggt cttttaccca acggcctttg accattccgc
     4741 catggatacg gtaatcaaca gtatggtcat ttttggcata ccattcatat tcccaaccat
     4801 tgtcatacgt atagataaag tgagttccaa taaaatcttc caattcttta aattctttca
     4861 tgtttaagca tctccttata aattaaaaca tgtaataaac tacatgtttt cgattactca
     4921 tttatgatag aatggattta aagatttgtc aaggagggaa aactgatggc aagaaaaaat
     4981 acacttcgat atattttgtt agggttatta agtaagaaga agatgacagg atatgaattg
     5041 aatcaatcgt tcaaaaatga aataggagaa ttttggcaag caaaacatag tcagatttat
     5101 cctgaacttg caaaaatgga agagcaagga atcattcagc atcaagtaga gatcacaggt
     5161 gaaaaattgg aaaagaaagt ttatgaatta acagaagaag ggaaggaaca attggcagag
     5221 tggattcata cacccaccaa tgaactaccg gttaatcgag acgaatttgt tctgaaattg
     5281 tatttcgtca aagacgtgaa tgatccggca ttagtagaaa ttattcagca gcagcgtgac
     5341 cttcatgaag aaaaacgact tcatctatta gagcgaaaaa aaacgatctt tcctactgaa
     5401 aaagaaaaac atgaaaatta cggacactac ctgattttag cgcatgcgat caatcgggaa
     5461 acagaatatg caaattggct aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa
     5521 aatatccgaa cagtgatgga gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg
     5581 gatattctta tgttcaaaca ggcctttttt caagtattta tgtagcatct acttattaaa
     5641 aagcttggta cttaggagat tagtggcgtt tagagttctt ttaacaactg ttcgtagtca
     5701 gctttttcat cgacaggaag gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg
     5761 atgtacatct tagcattctc ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg
     5821 tcgatcaatt cgatatcttt gattgctttt gaaaaaactt tacggacatg gtgcattttc
     5881 ttttggcgga tttctgcctt ttctgcagct tttgacaaag taaatccgtc atctaaatat
     5941 tttttgatca gttctacttt tatgactgta ggtaaacgat attttctagg acaattcgca
     6001 tcagcttcga tcgaacgaat gaatcccttt tgttcccaat accttaattg acgtggagac
     6061 acacctacga tatcacttaa ttcactgatg ccaacaacca attgatcatt ttccaacaaa
     6121 cgtttaaggg aagccccaac catttttgtc actccttttg tcctcttgct tcccttaatt
     6181 atatccgtat taacttatgt gtcaagtagg ttgacaaata gcgaatttag gactaaagta
     6241 ttctagtagt ttcagaaaaa attcagaaag aaggagaaat atgagtaaca accaacccgt
     6301 agatatttac ggaaaaccat ataatcggac acttttagtt gttgtcttat taattggtac
     6361 attttgtacc gttttgaatc agaccttatt gacgacagct tttcctacat tgatgaaagc
     6421 tttcgatatt tcagcgtctg atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg
     6481 gatcatgatc ccaataacag catggctgat caataaattc agttcaagaa atttatatct
     6541 ttctgccatg acgatctttt tgatcggaac gattacttgc tttacagcac ctaattttag
     6601 tacattacta atcggtcgtt tgatccaagc ttgcggtgtg ggagtatcga tgccattatt
     6661 gcaaaatatc atgctatcta ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc
     6721 agggatcgtt attggacttg ctcctgcttt aggccctaca ttatctggtt atatcattga
     6781 taactaccat tggcgtgact tgtttggtat ggtactgcca atcgttgttc ttgtcttggt
     6841 tttagcattc ttcctaatga aaagtgttat tccattgtca aatcctagca ttgatatttt
     6901 atccgctatt ttatcaacga ttggattcgg tagtttactt tacggatttt ctagtgtagg
     6961 agatgacggc tggggaagct tgaaagtaat tggtttctta gttgttggtg tgattttcat
     7021 cgccttgttc tcatggcgtc aattacattt ggaacatcca ttcttggaac ttcgtgtatt
     7081 caaatcatct acattcacga ttgcagctgt tttagctggt gtaacaaata tggcgatggt
     7141 aggggcagaa atggtcgtac cattgtatat tcaaaatatt cgtggagaat cagcattcca
     7201 ttctggcttg atgttgttgc cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg
     7261 tgcgattttc gataaatacg gcgcgaaacg tttagccatc acagggatgt tcattttgac
     7321 agcggcaact gcaccatttg ctttcttgac aaaaacgact ccagttttat atatcgtgat
     7381 cctttatgcg atccgtatgt ttgggatctc catggtcatg atgccggtaa cgacttctgg
     7441 tatgaatgca ttgccaaata acttgatcag tcatgggaca gcggtaaata atacattccg
     7501 tcaaatcgca agctcgattg gtacagctat tttgatcagt gtgttgacga atgtgacaaa
     7561 agcaaatatc ccaagcaaat ctgtattgga taatacgcca ttagcttata aagatcaggc
     7621 aatcaatgcc acactgtcag gttaccatgc cgcgtttatc gtagcgatca tctttgggat
     7681 tcttggcttt attattgcct ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg
     7741 ggataatgca tgattattgg agttttgatt gtcagcttgc ttgcttttgc tttaacgaac
     7801 gtgttcgcta aaaaaacatg gcaaaccttt ttatctttga ttttcggact aatcttcgtc
     7861 gcttcactaa gtttgatcgt cgctaatttg tccaatcact ttggaatgga aaaagtaacg
     7921 gaaacaaaaa cagaaaaaat tgtttccagt gctgattccc aaggagcaga tatgcttttg
     7981 tataaagctt tagggaatgg aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa
     8041 gaaaaaccaa aagcaactgg tacggataac gaaacaaata aagtagaaaa gacagacggc
     8101 gatgctgaaa aagtgacgaa aactacttat tgggaataca aaaacgacat gtataaattc
     8161 tggttcaata ttgctgataa caatcacgaa tatgacagcc gagtgaatac attcaaaatc
     8221 ccagagacat gggtagaatt aagtacggac caagcagcca agttagctga attagtgaag
     8281 aaacagcaaa gtacgatgga aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa
     8341 gcaatgactg aaaatccaaa aatgagcaaa gcagagcaag aacaacgtac gaaagcctta
     8401 gctgctgaat tccaacaaca agcattcgca aaattagtga aagaagcaaa aggcgaataa
     8461 tattttgaac accatgtagg aggttgtgaa aaagctgctc tgcatcaagc agtaagaaca
     8521 atcagataaa aatagcactc catatttttc atctgattgg gcttagtgcc gtagatgcaa
     8581 gcttttgaac accgtttaag gaagaggttg tgagcctgcc gtttaactct gagtcacaag
     8641 gagcaatttc aaaaaacagc ttctcatgtt tgttgactat tgtgacttgt gaccgaggag
     8701 ttggcaggag aacaccgttt attcgaaaaa aggaactgcg acaagacttt tgtcacagtt
     8761 ccttcttttt tttgctatgc tatactagat gtaaaaaata ggagaaataa aatgagggaa
     8821 atttattctt ttgaatcaaa aatctatgct tccgaagtcg gcaagggcgg ggcatatatt
     8881 atttttccgt atgatataag aaaagaattc ggtgctggaa gagttaaagt ccaagcaact
     8941 tttgacaatg tgccgtatga aggcagtatc gtgaatatgg gcgtgaaaaa tccagatggt
     9001 tcagtctgct acatccttgg actacgaaaa gatattcgta aaaacatcgg aaaagaaatc
     9061 ggtgatattg tagcagttac agtgaagcaa aaataaaaga ggttgtgaca aaagacgtgt
     9121 cacaacctct tgataaacgg tgttcaaaag ccagtttctc ggtagtaagt caaatttatg
     9181 caaaaatgtg gaaacccatg tttacaaaat ttttcttact actcgaaacg aagcggcttt
     9241 atcacaacct cttgataaac ggcgttcaat cagctgtccc tcggtattag ctcaaaaacg
     9301 gcaaaatatg agaagctatt tttccatttt tttctctcat actcaggtca taacgctgat
     9361 ttcacaacct cttctttcat aatggttttt caccatcttt ataataaact ggttatttga
     9421 tatggaaatc tattttttcc aaccaatcta aaatcttgta acctaactct tctgacatcg
     9481 gaaaagaggc gttgatttgt gcattggaga tatggtcaac atggataccg gaagtgatcg
     9541 tacaacttcc cggaacggca gttttgatga tcacagctat tttttcggct aagacatcgt
     9601 ctttatggaa ccgaccatcg tggctaggga aacgtacagt ttgcatctct gtatcacgag
     9661 aaaatgtagt gactgttccg atatgtggtg tatctccgcc tgttaaagtg atcaataagt
     9721 ctttaccgat tactttaaca gcagcttgga tcgtgaaacc tagtttggtt acttcaacta
     9781 tcgtttcttt ttccatatta atcaccctgc caacgttttg aatgctcatt tacaatttca
     9841 aattgatcca aaagtttcat tgtatggtga ttaacaagat cttggatcgt ttgaggctga
     9901 ttataaaaag caggaattgg tggtatgatt tgcacaccta gtcgtgctaa tttagtcaag
     9961 ttttccaagt gaatggcatt taatggcgtt tcacgaggaa caatgactaa ctttcgttgc
    10021 tccttcattg ttacatcagc agcacgtgca atcaggttgt cgcctaaacc aacagagata
    10081 cctgcgactg ttttcatact tgccggtacg atgatcattc catcggtcaa aaatgatccg
    10141 ctggcgacag cagcacctaa atctttgttt gaataatggt aatcaaacag gctttcgaat
    10201 tcagttagtg ataagtcgga ttccagcttt aagttctgtt ttgcccatgg actgatgatt
    10261 ccgtgtgttt ctatcatcgg atattctttt agtttcttca ctaggttgat ggcataaatg
    10321 gtgcctgaag cgccacttac accaatcacg atttttttct tccgttgtgc caattgttcc
    10381 attttccgca ctccttttta tgatgtcaaa acagttgcag atcattcatt atttcaaata
    10441 tttttcccaa tcttctactt cttggaattt tgcacgtaca aaatgttctt tcatatcgaa
    10501 aggaactgta ccgtcaaaga tagtttttgc acttatccca cggaaacgaa tagagccagg
    10561 attatattct ggtaattctg atgggtccaa tggatggttc ctcattcctg ggatcacgat
    10621 gatatcttta tctccttgga aacgagtgtt gaaagtccaa actacatcgt tcatgtcgaa
    10681 aatatcaaca tcttcatcga ctaaaatgac tgtcttcaat tctttgaagg ctgataaagc
    10741 aagaatagct gcttgacgct gaataccttc atccgcttcg ttttctttac ggatttgcat
    10801 gatggtcatc aatttaccac cacctgcagg aggattgtag acatttacta cttttcctgg
    10861 aatagcttta tcgaccaagg caagaatact tgcttctgta ggaataccgg ccatagatac
    10921 gtgttcttca ctgggaccaa tcgttgtttg catgattgga ttgtcttttc ggtgagtaac
    10981 tgctttgact ttcactactt gtacagctgg attagcatcc ccagtatagc caggaaattc
    11041 tggcattgct tttcctgagt tcgtgttgat atcttcttgc attgtttcat ttggcatgat
    11101 ttctgcttca atgatgaatt cagcacgagc aatacccact tcatcgactg ctataccatc
    11161 aacgatttga ataggttcgt tacgcaaagc tcctgcacac catagttcat tgtagccaag
    11221 tggagtggta ggtggttcga aagtcgtgcc aatggcaatt gctggatcta aaccaatatt
    11281 gatagtgata ggcattggtt tattcaaggc ttcgaattgt ttttggaagt gaccaatgtg
    11341 gcgtccgcca ggcatgatgt acataccgat cgtatcttta tcttctagaa ccatacgatg
    11401 gatcgttaca tcgctcattg ttttttctgg atctgatcct aagactacac ccattgtgat
    11461 gtatggacca gcatcttcag gagtatttgt aggtgcaggc aaaatgttgc gaatatcgaa
    11521 gtctgcatcg gtagctagat gaacgacttc ttgtgctgga gcgtctgctt tgtctacttt
    11581 cacaggtttg attggatttt ctacagcgtc tttcaagaaa cgaccaagtg ttcgataatc
    11641 atggtgcaaa atttcaccta cacgtttacg actagccatt gttccgatgt tcacacgtgt
    11701 gccggggaaa cctttgatat tgttgaaagt catagctggt ccttcttgag tcggacgttt
    11761 tactgtaccg ccagcaccga tataacggta aacaccagat aattctgctt caggatcgat
    11821 ttcaatatct gtttcgtgat attgacctgg aagtgttttt agttcttcga tgacttttct
    11881 taaatcatac ggttgatctg tcatgagaga ttcctcctta gttgtcttta cctatctata
    11941 gtaaaaggtg aaaaagagga aaacaattca acttgttcgg taaagtattc tacaatggaa
    12001 tggatgcgtt tttttgataa tagaataaac tatgatatac ttatcactaa aagaaagcgc
    12061 atacttagtg ttggaggaag gaaagtgaaa aaaatggagg agtcatttcc taaagcagta
    12121 aaagtggaaa atatagcgaa tattttgaaa gttacatttg aaaacggaga agtaaaatac
    12181 gtgaaatctc attggacaga agagataacc gatgccttgc aatttggaaa aaaaggaaga
    12241 ggaaaaagaa aaaatcttct cgcactttct cgtaatatgt ggattgggac tgaagtaacg
    12301 attgaagcag atggaacagt attcataaat ggaaaagaca ggtatacgcc agaagagttg
    12361 tggtacaaag gaaagaaaag tataccagaa ttataaaaaa taattaaaaa aaacagcgcg
    12421 aaacaaggaa catattactt cctcgtttct tgctgttttc tactatttca gcgctcttat
    12481 gactcaaatc taaatgtggt tgtcacaacc ttattctgca cgcaaagcga tagcggcgtc
    12541 ttttttcgct gccattcgtg caggaagatg accgccaagc atcgtcaata tagtagagac
    12601 gatcactaga ataatcgcat ggacaggatt tagttgagca acatttttta gatccgtcat
    12661 gttgtataag atgctattga tagggaacgt agctagccat gcaataaaca cacctaatat
    12721 cccagaagaa atacctaaaa tacacgtttc tgcgtcaaat acgcgagtga tgtctttttt
    12781 acgtgcacct aaagctttta gtacaccgat ttcttttgtc cgttcaatca cagatgtata
    12841 agtaatgata ctgatcatga tcatacttgt tactaaagag atccctgcaa aagcaataag
    12901 gacataagtg atagcatcca tcaatccgcc agtcagttca gtcatcgttc cagctaaatc
    12961 agtataaatg attttatctt catctgattt gcctttattg taatcatcta gataatccaa
    13021 gatcttttct ttgtcttcaa aattgttcgg ataaatcatg atacttgaag gcaagctgtc
    13081 tccacctaaa taactaagca atgtttgttt ggcattttcg tcaacttttt cagtagttaa
    13141 gacatttaca ccactgtctc tttgagcttt gacgatttct gattctttgt tttcgttgac
    13201 gatttgagtc gttaattgat cgctataagc gattcctgga gataagagat tcattgtaga
    13261 agaagatttc acgcgtagga tccccgagat cttcaattca tcagaagcat tctgatacat
    13321 cgcatcataa tctgtattag ggataaaatt cccagttgga agttttgtat agtaagtatt
    13381 gttgtttact aatttgaatg ttgtaccaac gatatcatca aaatcaagtt tttcgtcttc
    13441 tttcacatca aatccaagat tcttcaatgc gttgatatta gtattattat tgccgtctac
    13501 gatcagcacg acatctgttg ctgaggctgg ataacttccg gcaagtaaag aataattgtc
    13561 ttttaaaaag ttttctttgc tagtgtcaag ctgtgtaggg aaacttgaca ctcccacacc
    13621 tgtcattgct gacatcgtac tagataaaga aagagattcc gtatcaggat tttgattcga
    13681 gaagctgaca ggctgtactt tgccatttac atcacgtaaa agattgattc ctgtagtacg
    13741 ggtaaatccg atattattac ttaagttagg atcgatgtct gttacgtaat cgacgtatgt
    13801 ttgatcaatc ttattcgtgt gctgtgcttt atcttcttcg ctgacttttg cagtaatcgt
    13861 tttgctgtct ggataatccg ctttgcttgc acccaatccg gcatcgtcac gagtctggtt
    13921 agtcgttacc ttagaaatag taatcggaaa tttcgccatc gtttcagctt gcgtgttatc
    13981 gatttgtttt tggaatccag aagaaaggga cagaacgatc gcgattccga tgatcccaat
    14041 actggaagca aaggaggtca agaaagtacg tccttttttt gttcggatat tgttgaagga
    14101 aagctttaac gctgtccaaa aactcatctt tgttctgcgt aagttaaatt gatcatcttt
    14161 tggtcgttcg atgtgaggat gggaatctgt taagatctta ccatcagaaa attcaatgat
    14221 tcgatctgcg tattgatggg ccagttcagg attatgtgtg accatgatca ctaatttttc
    14281 tttcgataac tcttggatca attccatgat ctggatactt gtttctgtat ctaacgctcc
    14341 ggttggttca tcgcacaaaa ggatatctgg gtcattggct agtgctcgag cgatggccac
    14401 tcgctgcatt tgtccaccag ataactgatt tggcttttta tgcatatgat ttgtcaagcc
    14461 gactcggtgc aatgcatctt ctgcccgttt tcgtttttcg tctttagaaa caccacttaa
    14521 tgtcatccca agttcaacgt tttcaataat gcctagatga ccaatcaagt tgtaactttg
    14581 aaaaatgaaa ccgatagagt tgtttcggta ggcatcccag tcactgtcct taaagtcttt
    14641 agtagacttt ccgttgatta ccatatcacc ggaatcataa ttatctagtc caccaatcac
    14701 attcagcata gtcgttttac cagaaccact aggacctaaa atcgctacga attctttttg
    14761 tctgaaagcg acagacacac catccaatgc tttggtggtg gtttcgccga ctttataata
    14821 tttttttata tcttttaatt gaagcataat cacccttctc ccgtattgaa atattcggta
    14881 tactaataag tatatcagaa caatgatcgt agtatacgaa ataaatgtta actgaagatg
    14941 aactacaaga aaaaatctag aaatttttca aaaaacagag ggagatcaat gaatcgtatt
    15001 ttagtagtag aagatgatcc gcaattacag ttgctttacc gttctgtgtt ggaacgagcg
    15061 ggcttttcag tcatcaccgc attgaatgga gaggatgcgc tgaaaaaact agaagcgaca
    15121 cgtatagaat taatcattac agatatcatg atgccgaaaa tggatggcta tgacttgcta
    15181 gactcattac gtagcaccag atttgacaca cctgttctaa tgattacggc aaaggccgat
    15241 tttgaggata agaaacgcgg gttcaaatta ggagcagatg attatatgac aaaacccatc
    15301 gatgtcaatg agatgatcct acgtgtggaa gcattgcttc gacgttcaaa gatcaattac
    15361 agcagtagct tgataatcgg acaaacaaaa ttagagctag atgcttatac agtgttacaa
    15421 gatcagcatt cgttgattct gcctcaaaaa gaatttctgc ttctgtataa attactgtct
    15481 tatcccaaca agatcttcac tcgccaacag ttgatggatg atatttgggg attagatacg
    15541 aatacagaag aacgaacgat cgatgtacat atcaaacgtt taaggacccg ttttgaaaag
    15601 acggaagatt ttcaaatcat cacagtacgg ggactaggct ataaggcggt tatttaaaga
    15661 tgaaaaaaaa gctattttca caattatgga tctattttgc tagtattgtc ttcatttcga
    15721 tcttagtgac actgctgtgt tttctcggtt ttattttctt attgtctcga cattctatct
    15781 cgcctgctga atcggctgga aaaccaacgt tatacccgtt attcgtcttt gcggggttca
    15841 gtatggtcgt gggtacagga atatcgattt ttgttgggcg gcgtatcctg catccaatca
    15901 gtgcgctagg gacaaatatg agtctggtag caacgggaga tttttcgatt cgaatggatg
    15961 aacagcagaa agtagcagaa gtacagcaac tgtataaaga tttcaacgtt atggtccaag
    16021 aactcaatag tatcgagaca ctgaggaatg actttgtgtc cagtgtatcc catgaattca
    16081 aaacaccact agcaacaatc caaggatatg ttcaattgtt acaagcccca aatctctctg
    16141 acgaagaacg ccaaatattt ttatatcgga ttatcgaaag tatcacgcag ctttcacaat
    16201 taacagagaa catcttaaaa ttgaacaaac tggaaaacca gcgtatccaa ctggaaaaga
    16261 aagagtatcg attagatgaa caaatcagag aagtaatcgt tttttttcag cctaaatggg
    16321 agaaagaaca gctagaattg gatatcgaac ttgctgcagt aaattacaca ggcaatgaag
    16381 agtttttgta ccaagtgtgg ctgaatatca tggataacgc cataaagtac aatcaaatca
    16441 atggacagat tcatatcaaa ctatttgaga cagccacaga gattgtactg gaagtgaccg
    16501 attcaggtgt aggaatgaat gaagagacca gagatcgaat gtttgaaaaa ttttatcaag
    16561 gagatacaag tcgtcaaatt tctgggaatg ggctaggact ttcactggtt aaaaaaattc
    16621 tagaactgca tgatggacgt attgattata gcagtatcga aggagtagga acgacagcaa
    16681 tgatacggtt aagtaaacaa taggaaaaag aataaaaccc ctaaaaattc tattgaaggt
    16741 ttttaggggt ttttagtcgt ttttttgtga ttagggaaat gttcttacgg ttcttttggc
    16801 ataaaaatcg ctgctttctt attgaactac gagaaatgtt agaggtgtca ttgaaacaag
    16861 gacatgatgc aagaaagaga gcgaaagtca tgcgtcgctc tctttttttg ggtggataga
    16921 tggacagaaa atgtagggct tagttttctt cttgatttaa atactcccaa aatgtatctg
    16981 aagccaaaga atgctcaccg actttacgag taaatgcaag ataactctct gttgttggag
    17041 aaatagggag aatcacagta ttttctttta atagattctt gactgttttt tccataacaa
    17101 tagcaatccc catgtcattt gcaatcatat tgatgatcaa gtcgattctt gctcctttat
    17161 aagagatttt tggttcaaat ccagcttcgc gacacagatc tagtacaggt tgatacaagt
    17221 tcgtagtggg gccaaggagc aaaaagttct cctttttcag ttcgcttaga tccaatttct
    17281 ttttactagc taaaggatga tttttaggta gaactgctac aaaatcgtct gtttcagtag
    17341 gcaaccactc aatcgaactt ggtggggcct ggaaacttct agcaaagtgg atcgtgtcac
    17401 tatgttcatt ggcgtcattg taagttaatt cgacactttc cacttctttc aactggactg
    17461 taaattcagg atggcgtttc aaaaactcgg tgattttgga aaaaccttta taattgatca
    17521 ttgtcggtat cgttaagata tttaatgaaa gattgttgtc tttcgtatag gtttcaagca
    17581 cttcttgcat agcatggtat tgttctacga cattttttac atatgggagc aataagcttc
    17641 cagcttcggt caattcgatt tttctatggg cgcgttcgaa taatttgacg tctaattctg
    17701 tttctaacga aagaatctgt ttagaaatat ttccttgggt ggtgaattgt cgatcagcag
    17761 ttttggtgaa attcattgtt tcggctaaat caagaaaaac ttcgaattta tggatatcca
    17821 tcatattcct cctacttatt ccaataaaga atactttaac acaaaaaatg aattggtgta
    17881 atcttctctg tctgttacaa tctttcttgc agcgaaagca ataattacag aaatttttct
    17941 gatttttttc gcttcgtaga aaaagagagg agttttttga tgccaactgg agttttgatc
    18001 aatgtaggtt ccgttttgtt tggcggacta attggcggat tagtaggaaa taaattaagt
    18061 gaacatttta aagcacagct gacgatggtt tttggtgtgt gttcgatggg gatgggaatc
    18121 tattccattg cgccgatgaa aaatatgccg gctgttattt ttgctttagt gataggaaca
    18181 gcgattggtt tgattgttca tttaggtaat gggataaata agggggctgc gctgatgcaa
    18241 gtacctattt ctaaaatctt ccctagtgaa aagcttggta tgacccacga tgaatttatc
    18301 agcacattag tgacggtcat tgttctattt tgtgcaagtg gtacggggat ctatggaagt
    18361 ttagattctg ggatgacagg agacagtacg attttgattt ctaaatctgt ccttgatttt
    18421 ttcacagcag ctatttttgc gtgtaattta gggtatgtcg tttctgtgat agcaattccc
    18481 caattcatta ttttttatat tttgttctta ttggcaaaat ttatttatcc attgactaca
    18541 ccagacatga tcttagattt taaagcttgc ggcggattct tgatggtcgc taccggtttt
    18601 agaatgatca acgtgaaaat gttcccagta gctgacatga tacctgcaat gatcgttatc
    18661 atgccactga gctggatgtg gacaaattgg attatgccac tgttataata aaaatcaaag
    18721 agaagccatt ttttgctgac taaaaaggat caaatctttt agtcagtttt tttgtatttg
    18781 acaaactgat tttgaaacta tattatatgt tgtataatat agattgaaat aaaatattac
    18841 acttgagaat agaaggtgac gtaatgcaca tccagattcc aactgttctt cttgatggta
    18901 ctgtgctagc agcattaagc aaggaagata tgtatgggta tgccttaaca aaaatcgtac
    18961 aagaaagtct ctctgtcagt gaatctacac tatatccggt tttgagaaga ttgaaaaaag
    19021 aaggatatct tgagacctat gatgaacctt ttgaaggcag aaaccgtcga tattactcgt
    19081 tgacaccaat gggaagtcgt catttagaag aaattattga tgaatggatt gtttttcgag
    19141 attctttaga ccggttattg gaggagagca caaatgaatg aatatttgaa tgaagttgtg
    19201 gatcatcttc aagaaattcc tgaagacgat cgttttgatt tgatccagta ttatgaggaa
    19261 tattttttag attctggaaa aacattagat cagatcattg atgaatatgg tacaccaaag
    19321 cagtttgcct taaaattaaa aatcagttac ttttcagaag aagaacaagc agaaaacgag
    19381 ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt taatcattat cggtttattt
    19441 gcttctcctg tcctcattcc actagcactg gggtttatat tggttattgg gggactattg
    19501 gttgctttac tagcgctcat ttttagtatc tatgttttat gtatcagtat cttaggtgtt
    19561 ggcctattca tgatcatctc aggaatcagt gtgtttggac aatcgattct gagtggatta
    19621 ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag tatttatttc gccactgttg
    19681 ctgaaaggaa caaaagggct tttttatgtc atgatgaact ttgttaaatg gatgggacga
    19741 cgttttatta caaaaagaga tctgcattca gcgggcgttt gaaggaggac acataaattg
    19801 agattgaaac attttttgat taccggtatt ggtctgatgc ttactggtgg cctcctagca
    19861 ggtgcaactt atacattagg cgctcaaaag agtctgactt gggataatgg tcctcggttg
    19921 atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca
    19981 gaaaatcaaa cagtttcggt catacgcgga ttggattttg agataaaaac gacttatgat
    20041 aagacagaaa agccagaaat aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa
    20101 aaatcaacag gtgcaatcat tagtttagaa gacacaagtg cacaaatcac tattgttgtc
    20161 cctgcagatc atgaattatc tgaattgaca atcgaaggga ataatagtgc aattgatatc
    20221 aacgaagtat caatcaaaaa agtgaatact aagacacatg atggctgggt atcgttcaat
    20281 gaatttacag gaggaacaat aactgcgaaa gatgaattag gctcacttcg attagatgag
    20341 gccaatgtgg ataacttgat cgtcacaggt gaaaacagca acttccatct agaagacagt
    20401 ttgatcagca aagaaggaaa agtcatccta aacggtggca atttatcttt gcatgagatg
    20461 gaaaacagtg gctatactat ccaaacgtct ggtgaatctt atattgaaaa agactaccag
    20521 ccaataacaa atactttgtt agaacaagga cagaaaaagc taaagatcac tgctaaagac
    20581 acatcgatca atattacgac ggatcaagag gacaatgaaa cagattgatc ctgtactcaa
    20641 ctggatattt taagaaaaaa cagcaaagat ccttccaatc tttgctgttt ttcatcgctt
    20701 aacgcacata actatagtaa aataagaaag aaaatatcta aaaagaaaac agggtgaaag
    20761 agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa aacagaaatg gaaagattac
    20821 atacttatat cactcaacta agccaaacct tctatgatcc agacaaagaa aaagtgatgg
    20881 ttaattaccc gaacaattca gaaggtcgcc aattagagca agtgtaccat gaagttttca
    20941 aacatttatt gacggtgaag aaagagctgg attattattc tttaccgatt atcgatacag
    21001 gaatattgaa gtatgatggg aagaaggaac gatttatctt taaatctgtt cgggaagatt
    21061 tgcctctttc agcagggatg gatctagagg tcttggtaga agactatttt acagaagaaa
    21121 agcactgggt tcgaacaaaa ttggattatt taccgcaggc agccggaggg acacaggcaa
    21181 gcggttggta catcactgaa gacaaagagt tagaactaga aggtgtaatg gcgcgtattc
    21241 gaaaaaaaaa ttaaaaaagt aacattagat atttattgaa ggataaagga gcgcaggaag
    21301 tagcatgaga gaaaaaagag cattttatat caatggttat gcagggattg tcggattggt
    21361 cgtattagct ttagttggat tgtttttctt ctatttaggt atgtggcaag cgaaagtttg
    21421 ggcacttttt ttaagtattt ttctttggtt gatcacgtta ttgttgttaa gttcggcaac
    21481 tgtagtcagt cctaatcaag caaaagtaat tttgtttttt ggacaatatt taggtacgat
    21541 tcgagaaaat ggctttttcc ttacgatacc tttggcacaa aaaatgactg tttcgttgaa
    21601 agttcggaat ttcaatagtt ctgttttaaa agtcaatgat ttggatggaa accccatcga
    21661 aatctcagca gtagtagtct ttaaagtcgt tgatacagca aaggcattat tcgatgtagc
    21721 gtattatcaa gattttgtag aaatccaaag tgagacagcc attcggcata tcgctagcca
    21781 atacccgtat gatacattca atgagaatga tttgacgctt cgaggaaaca cgacggcagt
    21841 gtctgacgag ttgcaaaaag agctgcaaga gcgtttagca gtagcaggtg tcgaagtaat
    21901 cgaaacacgc ttgaaccatt tagcctatgc aacagaaatc gctagtgcca tgctccaacg
    21961 tcaacaagca aaagccattt tgtctgctcg acaaaccatt gttgaaggag ctgtcacaat
    22021 cactcaaatg gccttagagc aaatcgaaga tggacaagaa atcaatttca ccgatgatcg
    22081 gaaagtccaa ttgatcaata atttacttgt ctcaatcatt acagacaaag gaacgcaacc
    22141 tgttatcaat acaggcgaca tgaaagaccg taagtgacat actttttgta agataggaag
    22201 aaggagttta ggacggatta ttgtcttgaa ctcttttttt agttgtttta aagaaaatga
    22261 caaaattgaa agaagaatga tagttgattc gaaggcagaa ttgttcgctt ttttctataa
    22321 tcagtatata aagtaagaga gaaggggaaa aagtgaaaaa acttctgatc ggtctacttg
    22381 ttttcatcgt tgtggtactt gcaggggtga aaatcgcaga ttacgtagtt atgggtggag
    22441 aagaatacta tgtacaaatt acgacagatg gtacaaaaac agtttcaaaa tcagactctg
    22501 gacaagaaac agtcaactat agatatatcc tgcctggcta tacgaaagag ggtaaggaaa
    22561 aagaactgga cttcaaggga caattgactc gaccgcttcg taaaggagca tatttaaaaa
    22621 tcacctggaa caaaaataaa ggtgtcactt cgtatgaaga agtcgaaaaa aaagacgtgc
    22681 cgaaaacagc actggaaaaa ctaattaaag ggtagggaaa gaaatgaaca aaatagtcga
    22741 agtacagaat gtaacaaaag tatacggaaa aaacaatgag aaaaagacgc aagcattaag
    22801 cggcatcagc ttcgatgtag aaaaaggcga atttattggg atcatgggcg cttctggttc
    22861 tggtaagtcg acacttttga atattttatc cacattagat aaaccaacag atgggcatat
    22921 ccgaatcaat caaaaagatg tgaccacatt aaaaggaaat caactagcag attttcgtgc
    22981 gaatgagatt ggctttattt tccaagattt caatttatta gaaagcttga cagcacaaga
    23041 aaatatcgct gttcctcttt ctttacaagg cgtcagacca aaagaaatca agcaacgtgt
    23101 ccaaaaaatt gcagaacgtt tatccatctc tcatattttg gaaagctatc cttctgagat
    23161 ttccggtgga caaaaacaac gggtcgccgc cgcacgtgca ttgatcacac aaccaacgat
    23221 tctattaggg gatgagccta caggtgcgct ggattccaaa agtgcccggg atcttttaga
    23281 tacgatggac gaattgaata caaaagatca tgtatcgatt cttcttgtga cgcatgatcc
    23341 gttttctgca agttattgcc aacgtatttt attcatcaag gatggcggta tccatcaaga
    23401 agtgaaacgt ggagatcaaa gccgtgattc cttctatcga gaaatactat ctattcttgg
    23461 aaacttggaa cagtaggggg agattgacta tgctttggaa attatcactt actgggatca
    23521 agagccgttt aagagactac ttggtgttat tctcaggatt agttatggca tctgctattt
    23581 tttacatgtt tgaatcaatg gcaagcaacg aagctttttt gaaaagtaat acgatcgttg
    23641 cggctacagt tatcattttc cgattcggtt ctgttttact agggatcatc acattcgttt
    23701 atattttata tgcaaattcc tttttgatga cgatgcggca aaaagactat gcaatgttta
    23761 tgatgctggg agcaaaaggc agaaaaatcg cacagatgat tttttctgaa acattcttag
    23821 tagggatcgc agctacttta gtgggttcag ctgtcggtgt aggattgaca agtatcgtcc
    23881 accgattatt agtggatcag ttagatctca atatttcgca ttttacacct tttagtattc
    23941 acgggttgtt gatcactgtt ttgttttttg ctgttttgtt tttattagct gctgttgtca
    24001 atgctttttc gattgtcaaa aaaccgattt tagcattgat tcgtgcggat cagacaccaa
    24061 caagaatcaa gcagcataaa ttcttgtttt tccttgaagt agtactggga attgcttttc
    24121 taggctgcgg ttactatatg atgaaacacg tggggaccta tcaagtcttt ggtatagggg
    24181 tagccttagt tacgatcgta ttaggaactt atttcatttt ccattctgtg atcattttct
    24241 ttttatcatt actaaagaaa tcagaacaga tttcattgaa aaaattgaac aatttcacat
    24301 tgtcacagtt aaactttcgt attcgtgaat atacacaaat gctctcaatg gtcgctatgc
    24361 tgtttgcctt ggcactgggc gctttgacgg taggattagg ttttaaaaat gaaattgcag
    24421 acttgacaga aaacaattct ggatatgatc tagtgatgaa taatcctcgg gcagaagatc
    24481 agcaaaaaat caaagaactt tcaccaacac tttctgcttc ttatactcaa aaagaagatg
    24541 cgggaacgat ttactataat gcaagtgaat ttgcggctca accattgatc atgataaaac
    24601 atgaagatgg aacaccgccg aagatcacga aagaaaaagt tcctgctaaa aaaatgaatg
    24661 aactgactgt acaagaggaa cttcgaagct atgaattgcc ggaacagcaa gaaaaagaaa
    24721 tcaaggtagt cagtcaatca gaatttgatc aactgaactt gccacaatcc actttacagc
    24781 tagttcaggt aaaagacttc caagcaaacc tgaaaccaat cgaaacactt gttgctcaaa
    24841 ataaaatgaa caatccatct ttgcaaaaag tagagtacag caaccagaaa tatgaaatgt
    24901 atgaagtgta taatggtcta ttctctggtt ttgaatttat gggattcttc ctaggtttag
    24961 ctttcttgac catgcttgct agctgtttga tgttcaagat cttgtcagga agcaagagtg
    25021 acattgctcg ttatgaaatg cttgaaaaga taggaacacg acgcgggttg cttaaacaat
    25081 cgatccgtag agaagtaggc gtgttattct tagcaccagg aatcttaggt gtagtccatg
    25141 tcttattcgg attacaaatg tttggtctat tcatgcaaaa tccatacaaa gatatttggg
    25201 ttccgtttac gatattcttc gtattgtatt tcatctacta tgtactgacg acatggttat
    25261 atactggaat cgtattgaaa aagagattgt gaaaaagctg tcctgcatca agtaataaga
    25321 acagccaagc aaaaatagct cctcatattt ttcgtttggt tgggcttatt accgcagatg
    25381 caagcttttg aacaccgttt gttcggaaaa agaggttgtg acaatatttt tgtcacaacc
    25441 tttttttcac ggtcttttat tagaatatag taaatttttt ctcgttactc acggaataag
    25501 ctgttaagaa aacggtttcc atgtatactg gaatcagtgc aaatgaaaga aaagagggtg
    25561 caaggaacat ggaaatcaat gaacaaaatt ttggcgaaaa ctatcacttg attacgatca
    25621 cgaatcaaaa tgggacatct ttgtctgtca gtgatcttgg ggcacgggtt gttcattttc
    25681 aaacaataat cgataatgaa aaacgagagt tgatcttagg ttttgatgaa gcaaaagaat
    25741 atctagaaaa agacccttat atcggtgctt caatcggacg tatggctgga cggattacga
    25801 atggcacatt caaattagat gatgaaacgt tcgaagtaaa aacagacccg gacactggtc
    25861 attgtttaca tggtggtgca cctagctttg aagccaagca gtggcattat acagtcatca
    25921 atggtgaaaa cgaagcatcg gtcattttct atcagactag cccagatatg gaaaacggat
    25981 ttcctggaac tttagatgta gaagtacggt atacgctgac gaacgatgat acatggcgag
    26041 tgacggtcca agggatcagt gaccgcaaaa cagtattcaa tcctacaaat catgtctatt
    26101 tcaatttaac cggtgatcca gcacagtcaa tcgacgaaca tatactttgg ttgaatagcc
    26161 gattatttgc tacgctcaat aaagatacaa cgcctacagg agaagtagtt tcagtaagtg
    26221 gcacagcatt tgacttccaa cgtcctaaaa aattaggtga ggtctttgcc agtgattttc
    26281 gccaatcaat gatagtgggc ggcatcgatc atccattttt gttgaaaaaa caatctggtt
    26341 tactaaatgc tgcggaattg acaagcccag atgaaaaagt atcgatccaa gtaaaaacag
    26401 atgcttcagg aatcgtgatt tttacagcaa attttggaga agatggaccg gaaatgcgcg
    26461 ggaataaatt agtaaatcat ggcggtatca catttgagac tcaagaactg ccaggagcgg
    26521 agcgacttga aagttttgga gatgtcactc tggaacctaa ccaagtacgc gagtttgtaa
    26581 cggaatacaa aataaatata aagaaataga ataaattaaa aagggagtgt gacagtgtca
    26641 catccccttt ttaatttatt ctatttcttt aaaggcagat caagccagaa tgatttgtct
    26701 aaacttaaca aggttagtac aggaacagtt gcagctttaa ctgtacgagt agttgtttct
    26761 ggtatctgtc ttgccgtaaa attgttccac aaatcccaga ttgaagcgac acggaagatg
    26821 gaagaagcgt tgttgacgcg ttttatcgtt gaaaattcta atcctgtgat tggcacgcca
    26881 tctgttgtcc atttgattgg cacatcgaat cgcttagaca tattgaggat ttcagcgttt
    26941 gcttcttcag ttgttgctgc ttcttttttc ggttcatcat attttgtcag gtcgtacgct
    27001 tcgatgatcg cgttcaattt ttgtgaatat tggcgatctg tagcataacg tccttccaaa
    27061 tatttggttg cttctttgta gtcttttgtt tccgacttcc aagttggttt gtagaaatca
    27121 ggattgccag tcagtccatt tttgatcaag tcggcatagt cttccaaact ttccttgtaa
    27181 gaaggatatt tacggaattg tgcgcggatt tggtagcttt gtccggaact atcttgttca
    27241 agtgtattga aggtcacgct gcttccttta tatgatcctt tgataccaaa aaggttatga
    27301 tttggttcgc ttgataataa gctgtttcca gatccacttt caaggatagc ttgagccatc
    27361 atgactgatg cgtagattcc ttctttttgc ccgatttcct gtgcatcatc tgcaatcttt
    27421 ttcaagaact gatcagttgt ttggttttga ctgaaatgga acgagccatc taatccatct
    27481 aaaatagatg agccgcctgt cagcgaacct gaagcagcag gtaaacctgc taagtttact
    27541 cggacagcaa aatcaggcgt gaaatctgaa atagaagtta ctcgaacttt atctcctggt
    27601 tgaggcgcat ggataaattg tccgttccca atatagatac ctacatggtg agtaacaccg
    27661 gaagaaggtc cccagaagac gaggtcgcca ggttgggcct gacttactgg aatctgcgta
    27721 cctgcatatt gctgttcgcc tgtccagcca ccgatataat gaccggttgc tttcatatat
    27781 acgtaataag tcaaaccaga acagtcaaaa gtatttggac cttttgctcc ccatacatat
    27841 ggttttccaa gatgcttgaa tgcttctcga acgattgctt gttggacatt cgttgtattt
    27901 gtaggcaata cagcatcaat tggtgatttt acaacaaaat cagaagaagc agaagatgga
    27961 ttctctatac cgctttgatt tgctgtattt ggacgagatg aagcattgtt cgttccgcca
    28021 ttcgatgaac cattgtttgg atggtttggt tctctctgag tatttccatt gttattcgat
    28081 ccagatgggt ttgtttgatt cgaagaattc ccgctgttcg aattattgtt attattgttt
    28141 gaattatttg aaccattatt attgttgtta ttacccacat ctggtttgtt tggcgtttcc
    28201 ggatctggtg tactagggac ttcttcacta ggatcagttg gtttctctgt tgaagtatcc
    28261 ggtccagctt taaattctgc agtaatagat aaattgttcg tacctactgt aaaggtcttt
    28321 gcatcataat ttacttcttt ggcaccatta acagcgactg aagcaatttg ttcaccttca
    28381 gctggcgtaa tagtgtaagt taccttcgtt tccgctaata aaccttctac cgttccactt
    28441 tccgtaatat ccaaaggttg ctgttcaggg gaatccgctg tcgaagcagt cagttggatc
    28501 ttaccaatca aagaaggagc aattgtaagg gaatatgtct cggctggctt ttcattttct
    28561 gatggatcag ccggatcaat ttcagttggc tcggtcgatc ctgttgttga gttagttgtt
    28621 ccagttgggt cttctgtaaa agaatccgtt gtttcactag tagctgagct ggatgtagtc
    28681 gtatctgttg tgactgtttg atcagctatt tgattatcta cggcaaatac ggtattggtc
    28741 gacaaaacag acggagcaat cagccaagta gcagctagca aacgtatcgt ttttttattt
    28801 ttcataataa actagtcctt ttcctttcat tgattttctg tcttattcag cattcattag
    28861 acattttttg gaacagataa tcgtgtcgaa aagctttttc ttttcataaa tctgtctgtg
    28921 tattctaaat aaaacaattt gtaaataagc aacgagcatt cattcgtttt tctttgacgt
    28981 tcattttatt gcatagacca cctttgtttg gggataaaca agtggggata aaaatggtta
    29041 tgcatatgta aattatacca aagtttggaa agtatcacaa aaatttataa aaataatgat
    29101 cctaaaaaga aaaagactga aatatgtgat aaacatctca tacttcagtc tttttcatta
    29161 actattcata agacattagg tcttcatcaa acccgctaac gagagtagtg atcattgcta
    29221 cgactagact aacagccagt cctgctaagt accagaaaaa agtaccggaa atgaatgcag
    29281 gcaatgtggt cagactacca aatacaaaag cgatcgcttt cacttgggca aatcccatta
    29341 cagctccacc agcagctgca ccaatgcaag cagcaacaaa tggttttttc aatgggatcg
    29401 caattccgta aacagttggt tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac
    29461 cggagatcga acgggttttt gaacgtttac ttcgcagaaa tacacctaaa gctgcaccag
    29521 ccattccaaa attggctgct cctgtcaacg gaacgattcg gtcgaaccca taagcagaga
    29581 tattactgat catgacaggg tttacagccc attggatacc aaatattacc aaaatattcc
    29641 aaccaccagc gatgattgcg ccactgatcg gtccgttaat atccatcatc caagtaattg
    29701 cggcagctaa accttcgcca aggtaaacac ccgcaggccc gaataccatt aacgtcaaag
    29761 gaaccgtgat aactaatgaa agtaaaggta caaatactaa ttgcatattt tcgcgaatat
    29821 attttttgag gaatttttca acaaaagaat aaaagaaaat acctaaaatc gccggcatga
    29881 cagtagacgt atactgcatc agtactacag gaatccccag aaaatcagta gctgctttgc
    29941 cactgtctga aagtaaagag gtcagattcg gttcaatcag tgctgcagca atgacagccg
    30001 caatatatcc attcacttta aacgttttgg cagcagaaaa agcaagaaag attggcagaa
    30061 aataaaagac ggcattcgac gcagcagaca aaatatgata agttccgctt gtttcactaa
    30121 gccagccaat cgtagtcgct aaaacaacca gaccttttaa tataccagaa ccggcaaaca
    30181 gcggaatgat cggcgtaaag atttccgata tcaaaccaaa tagtttttcg atcggattta
    30241 gcttttcttt cggagctgaa tgatcgattt gacgcgtcaa ttccttgtaa gcaggagcca
    30301 atccctgatc catgattatt tctgcatatc tatcgagtaa aatgaaatac ttaatggaag
    30361 ggatttgctg ttttaatttt tctgcattgg ctactggctg ttttttcaat ttgatacgaa
    30421 gccgagtcgc acatggaagg acagaatcaa tattgtcttt tcctcccaac tgttcaatca
    30481 gcatatgaat ctgctcggac agtaccatct gttcaggatt cattgtcgtt tccattagaa
    30541 gaccactcca ctcactaata taatattggc gtaaggtgtc atttcgcctg tacgaataaa
    30601 tgcatgacag ttattcaact cttttttcat ttcttcatgt gggataaagc taatcggtgt
    30661 atctggtaac aataatttga ttttttccaa tatttccggg ttcattgtct ttatttcttc
    30721 cgccaattca atcgattgta cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa
    30781 acttggaaga ccgtttgaga cagccaaatc tattttttca gtaccaaagg ggacaggcat
    30841 gcctgcatcc ccgatactta atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat
    30901 atctgaattg attacttttc cttttttcat gtttgtttct tcctctcatt gttaatttta
    30961 gactgctgat tgttctaaag cttcgcggta agggatagaa ggttgcgcac cataagactg
    31021 cacagcaaca gaagaggcta gatttccata aaggatcgct tctttcaagt tactaaggtc
    31081 tggctgaagt gtgctgctta atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac
    31141 ggcatccact ttaaaagcag ggacgatacc gtgttctttt tctgtatgat agaaagcgcc
    31201 ggcactgcct agtgtaatga ttactgttcc aatacctaat tcatggagtt tttcggctgc
    31261 tgcgaccaaa ctgttatgat ctgtcactct gattcctgta atgatttctg tttctgtttc
    31321 atttggtata atgatatctg tcttttcaag tagttcagta ggaatcgttt cacgagctgg
    31381 tgcaggattc aggatcgttg tttttcctgc gtcttttgca atagaaaaag ctaggatcgt
    31441 cgcatctaag ctactttcaa actgagcaat aataaagtca ctgtttgtaa tcagttgctt
    31501 gttttttagg acttcttgtt catggaaagc attatttgcc cctgcatgga tcaagatact
    31561 attttctcct gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc cttggatttc
    31621 agctatccca gatgtatcga tgttttcttt tgtcagtaga tctaataact gttgtccttc
    31681 gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt tggctgccac
    31741 tgcttggttg gcacctttac ctccgccagc gtgaaaaatc tcatgcgcat gcatcgtttc
    31801 gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac ttccaattac
    31861 tgtaaccgtg ttcattttac aaacctccaa ctatctcatt tattttgatg ggaaaattat
    31921 tttttaaaac gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa
    31981 agctatttca agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct
    32041 tcccattctt tttctgggaa ttggatccga tctaacaata acttggcaga tgtttgtccc
    32101 aattcgaaga ttggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct
    32161 atattatcat agccaataat gctgtagtct tcagggatgc tttttccagc ttcctctaat
    32221 ccgcgatata atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa
    32281 tcaagaagtt ctgctgtgat ctggtagcca ccttgttttg agaactgtgt aggtaagagg
    32341 atcagttggt cgtgagggat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga
    32401 gcgtggacat tttccggtgg attttcagga tagacaaggg cgatcgtttg atgtcctaga
    32461 ctaagcaagt gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca
    32521 ctaaatcctt cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa
    32581 ttttcgttga ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct
    32641 cgacgtgtga gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat
    32701 aatatcgtga cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg
    32761 aagaatggat tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc
    32821 atttgtctag caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc
    32881 tgaacagttt tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg
    32941 attgaaacgc cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc
    33001 atactcctat ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa
    33061 tcacgtgtgc cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc
    33121 gtactcgtgc agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac
    33181 gatcactgca agcatcttca gcaagcacga tatggtaccc gtgttgaaag gcatctaatg
    33241 cagtagcata tacaccgttt gttgtcgcta caccgcttaa aatgatagtt tcgattccac
    33301 gccggcgaag ttgaagatca agagaagtac caaaaaaagc acttggattg tattttgtga
    33361 tagtcaacat attatctgta tctttcaaag aatccttcaa aagtaaatca gtgaattctg
    33421 gaggtacaga agcagtcatt ccacccatat cagtttgttg actcaaataa gggaaagtgg
    33481 agatgtctac gttcactaaa actttcaagg cagcggtacg tttcaatgtt cttactaaca
    33541 aatcattgtt tgtcagaata gatgctgcag tgtgtggaga gagtgtaccg ttatttacga
    33601 tctctttttg catgtcgatc gcgacaaaag cagtttttga taaatcaaaa atcatatttt
    33661 cctttaaatc attcatgatg ttccttcttt caaataacaa tgttttttat gtaagtaaat
    33721 cataaatcgt ttatttgtca actaaattaa aggattcctt tctttagtct agagaaattc
    33781 agtggtaaaa taaataagtg tgagaaaacg cattcgatgc atagatatag cgagaaatat
    33841 cgtgaaaatg gaggaatcag agtgaagtat gtcgaagtaa aaaatgaata caaacgatac
    33901 aaaatgggag aaacgaccat tacagcaaat gacgatattt ctttttcaat tgaaaaaggc
    33961 gaattagtga ttatacttgg tccaagtgga gcaggaaagt caacgattct taatatttta
    34021 ggtgggatgg atacacctga tgaaggtcag atcattattg atgacacaga tattgcgcaa
    34081 ttttcagata aacagcttac tgcttaccga cgaacagatg tagggttcgt tttccaattc
    34141 tacaatctcg ttcctaatct aacagcaaaa gaaaatgtgg aattggcaac ggaagtttct
    34201 cctgatgcgc ttgatcctgt agaggtattg aggcaagtgg gcctagctca tcgattgaat
    34261 aactttccgt cccaattatc aggaggcgaa cagcagaggg tttcaattgc tcgtgcacta
    34321 gctaaaaatc ccaaacttct tttatgtgat gaaccgacag gcgcgctgga ttttgaaacg
    34381 ggcaagcaag tattgaaatt attgcaaaac gctagcagac aacacggcaa taccgttttg
    34441 atcattacac ataattctgc tttagcgccc atcgctgata gagtcattca cattaatgat
    34501 gctaaagtaa ggtctgttga attaaatgat catccttcat ccattgatga gattgtatgg
    34561 tagggggatt ttatgaaaac aagaacatat cttaaagcag gttttcgcga aattcggcaa
    34621 tctaaaggac gattcattgc aatcatcctt attgtcatgc taggaacact tttatttgta
    34681 ggtgtcaaaa ctgctggacc tgtcatgcaa aaaacgatgg atcactatgt tggtacagca
    34741 ggactttctg atttgcaaat cgtttctact ggtggcttga cacaaaaaga tattgctcaa
    34801 gcagaaaaga tcccagatgc aaaagtagaa acaggaaaac agatctatta ttccaatact
    34861 ggcaaaaacg aagtgattca aatattttct tataacaaaa ataacaagca gaacaagcta
    34921 caattaactg atggaaaact cccggaaaaa ccaaaccagc ttgtattgga tgaaaaagcg
    34981 gaagatgaag ggtataaaat cggtgatatt tatcagattg attcagatga attgaaagaa
    35041 aaagagtata ccatcacggg ctttgtccgt tctccattgt tcatcaacaa tttggagaga
    35101 ggatacgcga acgtcggaaa tggtagcgtc gattattttg tctatttacc tgaatccgtt
    35161 tttaaaacag atatttattc tgtcttatat cttgatttta caaacgtaaa gaatttgaat
    35221 acgtacagca aagcttacaa agataaaatg gagcaaaatc aagaaaaggc agaaaagtat
    35281 ttacaagatc gacccgaaga acgcttagaa gagctgaaaa aatcggccaa cgaagaatta
    35341 gaaccggcac agcaaaaaat agcggatggc aaagcacaaa caacgcaagc aagaacacgg
    35401 ttagaagatg ctaaaaaaca gttagcgcaa cagtcagcaa ctatccagca gcttcctgaa
    35461 gcgcaacgaa caatcgctgt tcaaacatta tctcaacaag aagctcaact tgctgaacaa
    35521 gaaaggcaat tagttgagaa agaacgtgaa ctggcctctg cagaggaaga attgaatcga
    35581 aatgcagaaa aaattgaaaa tttaactaaa ccatcatacc tatacaatga acgttcagaa
    35641 aatgtcggtt ttcaagaatt tggtgatctg tctgatcgta tcgctgcgat tgccaatgtt
    35701 tttcctgttt tctttttctt tattgccgca ttgatcacct ttacgacaat gacacgtatg
    35761 gtagaagaaa accgcaggga aatcgggaca ttaaaggcgt taggttatac gaaagttgaa
    35821 atcgcaaaaa aatatgcaat ctatgcaagt ctagcctcag ggataggtat tgttttagga
    35881 acgatattag gaacgaatct tttgccaagg atcatttttg agctgtctaa cgagcggtat
    35941 gatattggga gtgcgcttat tttttatgac tggccgccga ttttacaagc agctgccgca
    36001 ttcttcattg ccgcatttgg cgctgcaatg atcgttttat tcaaagattt aagagaacgg
    36061 cctgctgcat tgcttcaacc gaaagcaccg aagccaggga aacgcatttt tcttgaatac
    36121 atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa tcagttaccg aaatctcttc
    36181 cgatataagt caagaatgct tatggcgatt atcggaattg ctggatgtgc cgggctgatg
    36241 gttgcaggcg tgggattgaa agattctcta agttctgttt cagccaaaca atttggaccg
    36301 atcattgact atcaagcaat cgttacattg gattcatcca cggaagatag tcgtcaaaaa
    36361 gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga tcctagctat tcatactcaa
    36421 accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc tgacagtgat cgtaccaagt
    36481 aaaacagctg atttggatcg ttttatccat ttaactgatc ttgaagagaa cccacgctcc
    36541 ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag aactatttga cttatctaca
    36601 ggagatcata tttcgcttta tgatgaagat caaaaagaat ggaaagtcga agtaaaagaa
    36661 atcgtgaaaa actatctagg tagctttatt tatatgtccc ctgaatatta tgcttcagtt
    36721 actgatcaat cgatgatgga taatgcattt ttgctgcgta cagataagat gacaacaaaa
    36781 gaagaagaaa tgctttccga gaaactgctt gccacagacg ctgtgacaaa tacgtctttc
    36841 atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga acttagactc ggttgtctta
    36901 atctttgtcg tattatcagg actgctggct tttatcgtct tgtataatct aactaatatc
    36961 aatatttccg agcgtgtacg cgaattatct acaataaaag tccttggatt ttttgataaa
    37021 gaagtcacga tgtacattgt tcgagaaaat atcatcttta ctcttttagg aatcatcgga
    37081 ggatttggtg taggatatat attgactgac ttcatcttgc aacaagcgtc tatggagaat
    37141 gtcatctttc cattagtgat cacatggtgg gcttatgtct tatcagcagg attaactatc
    37201 gtatttacgg ttattgtgat gattgttacc catttcaaat taaagcatat cgatatgatt
    37261 gatgcgttga aatcgaatga ataaaaacag aggttgtgaa atcagcggtt tgacctgagt
    37321 attagagaaa caaacggcaa aatagttttt catattttgc cgttttttga gctaataccg
    37381 aaggacagct gattgaacac catgaatcag aggttgtaaa aaagccgtcc tgcatcgagc
    37441 aaaaagaata ctcaaataaa attagctcct catgttttac atttgagaag actgattgcc
    37501 gtagataaaa gcttttgaac accatttata gaagaaacaa gaatattttg ctattttaaa
    37561 caagaggata gacaaataca tgtctgatct cttgtttttt attgggcaac agacctctta
    37621 gaatgtataa agataaattt tacaaacgct ttcggttcag ttataataat aaatgataac
    37681 ttgtatatat aagttgtggt aggtaataaa tcagttgaat ggaggtaaat atccatgaca
    37741 tttaaagaca aagtcattta ccaaatctat cccaaatctt acaaagacag taatggtgat
    37801 ggaattggag atttaaaagg aatcaaagaa aaactcccgt atctgaatga tctaggcatc
    37861 gacatgattt ggctaaaccc tatttatcca agccctcaaa aagacaacgg ttatgatatc
    37921 tctgattaca cagccatcga tcctatcttt ggtacgatgg aggaattcga acagctaata
    37981 gccgaagcaa agaaataccg gatagaaatc atgctcgata tggtgctgaa tcacgtttcg
    38041 acagaacatg aatggttcaa aaaagcacta gcaggggacc agcattatca ggacttcttc
    38101 attttaagag aagagccaac ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca
    38161 tttggagata cggggaaata ttatttgcac ttatatgatc ggacacaagc tgacttgaac
    38221 tggcggaacc aagcagtaag agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa
    38281 ggggtaaaag gattccgttt cgatgttatc aatgttatcg gtaaagacga acaattgaaa
    38341 aataataaag aaaatgatgg caaacctgaa tatacggaca aaccaatcac ccatacgtat
    38401 ctaaaagaat tgaaccaagc aacgtttggg acggattcca ctatcatcac agtcggtgaa
    38461 atgagctcta cgacgatcga aaattgtatc ttgtatacag aacctagtag agaagaatta
    38521 tcaatggtct ttaatttcca tcatttgaaa gtcgattatg aaaatgggaa taaatggtct
    38581 actcctccat ttgattttgc agaattaaag cgactatttc atacatgggg agaaaagatg
    38641 agtgagggta acggctggaa tgctctattt tttaataatc atgatcagcc acgtgcgctc
    38701 aatcgatttg tagctatcga tcagtatcga aaacaaggag cagagatgct agcaaccatg
    38761 atccatttga accgtgggac gccctatatt tatatgggag aagaaatcgg catgatcgac
    38821 ccagactatt catcgatttc cgactatatt gacgtagaaa gtttgaatgc ttataagtta
    38881 ctccttgaac aaggattcac agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc
    38941 gataactctc gaacacccat gcaatggtca gacacagaac aagcaggatt tactacaggg
    39001 aaaccttggt taaaagttgg aggcaagcta gaagagatta atgtagaaaa agaactatct
    39061 tctgaagatt ctattttgtc ctattacaag aaattgattc ggctgagaaa aacatatcca
    39121 atcgttgcac aaggagatta tcatgcttat gaagcaaatc atccggaagt ttatggcttt
    39181 cttcgccaat ttgaaggaca gcagctgctt gtattaacaa atttctatga taaagataca
    39241 aggatcgcca tcccagaagg attcacagaa gccaatattc ttatcgataa ttattccgat
    39301 actgttcttc atcaaagtct ggagctaaaa ccataccagg caattgcttt gctgaaaaat
    39361 taaaacaaaa acacaaaacg tcgttcctac gagctattct cgtaagatcg acgttttgtg
    39421 ttttatttaa aataatcttc ttcagaaaat gaagagtttt catactctcc tttatcgcca
    39481 taaaatttat tgtagcgttg tttgaattta cggaaaataa aagtaacgag taatttgact
    39541 aacgtataga taggaatacc aaaaataacg cccacaattc ccattagatc acccatgaca
    39601 agtaaaacga ttaagatcgt aatcggatgg atctgtaaac gatcacccat tactcgagga
    39661 atcaccaagt caccgtggag aagttgtacg gcaaaccaga caatgacaaa tttgacagcc
    39721 ataaaagtag agtcttggaa tgcaatgatc aaacctggaa agaaggcgat aaacggccca
    39781 atatatggaa tgatacataa caacccagca gaaatagcta aaacactagc aaattctaat
    39841 ccgatcagta agaaacaaac ccagtagact gctccaagga taaatgaagc aatgatttgc
    39901 ccctttagaa atgaacccaa ttgttgatta gcaatctttg tcaaattttt aaaatctgta
    39961 cgaaatgctg gtgggacaat cgctaaaact gactgataaa atttttgagg atctctcaaa
    40021 aggaaaaaag cgacaattgg accagtaaat aaagtaataa aaatagctgt aacagttgaa
    40081 aaaatgttgc tgatccgttg tgcgccactt tcccaataat ttccaataaa tccagctata
    40141 tcagacgtca cgtcgtcaat agaagcgaaa aattgattaa atgaatcggc aaaaggtgtt
    40201 ttctccaaaa attgtcggag actctcttga aaatcttgaa acaaatctgg caaactactg
    40261 atcaatgtct ttgcctgttc ttcaagaaaa ggaataagcc atatgcttcc aagccccaat
    40321 aaaaataaaa taatcacata aacgataatg actgacaagt tcctcgaaag gtgcttgctc
    40381 aaacgtttga tgactggttc caaaagataa tacaagatca gtgcaaacaa taatgggggc
    40441 aagatagtag cgataatgat tccgacaggc ttgaatataa acgagatctg atggaaaaaa
    40501 taaatgatca gcgcaagtaa gataactatt cctaaaacat agtaagaggt ttttcctcct
    40561 aaaaagcgga tcaaacgatt cgttgtatct gtttcttttt tcactctttt tcctccttta
    40621 tctataactt cattatagac aaaagcgggt ataaaaggaa aatgatagag gttgattgca
    40681 aaaatatcgt caatcattca tataaacttt ctcatcaatt aaaataagaa aacgcttgac
    40741 agttctttta accatatgat aaattaaatc ctgttgctaa aacgtaacta ttacggtttg
    40801 cgaaaagcga atcggaacat tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa
    40861 acgattgata ggtatattgg ccatgctggt tttagcaggt atgttaactg cttgcggggc
    40921 aagtgggaaa gccgaggaca gtaaagaaaa attgagcgtg atgacgacct tttatcccat
    40981 gtatgatttt acaaaagcaa tcgtcgggga tgaaggagaa gtagagttat tgattccagc
    41041 agggactgat tcccacgatt atgaaccttc tgctaaagac atggcaaaga tccaagatac
    41101 agatatcttt gtttataatg atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac
    41161 attacaagaa ggcaatgtcc atacgataaa agcgacagaa ggtatgcttt tacttccggg
    41221 aagtgaagaa ggacatgatc atgaccatga acacggggaa gaaggccata cacatgagct
    41281 agacccacat gtatggctag ctccaagttt agcaatcaag caggtagcaa acatacgtga
    41341 tcagctgatt gaagcttacc cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta
    41401 taccgagaaa cttcaagcac tccatcagct ttatcaagaa acctttaagc aagcaaaaca
    41461 aagatctttt gtcacgcaac atgctgcatt caactatcta gcgttagaat atggattgaa
    41521 tcaagtatcg attgctggtc tttcttctag tgaagaaccg tctgcggcga gaattgctga
    41581 actgaaacac tttgttaaag aacatggaat caactatatt tattttgaag aaaacgccaa
    41641 agatagtatt gccagaacgt tagcaaacga agcaggtgta tctttagaag tattgaatcc
    41701 tttggaagga ttgacgaatg aacaaataga aaatggagaa aactatcttt ctatcatgga
    41761 agcaaacctc gaagcgttga aaaaaacgac agagacagaa aacccgctag aagatacttt
    41821 gacccccaaa aaagaaaaga gcgtctacaa cggttatttt gaagacgagg atgtacaaga
    41881 tcgctctctt tcagattggg caggaacttg gcaaacagta gatacgtatg tagaagatgg
    41941 gacttttgat cctgtttttg aatataaaga aaaattgaat caggataaga cagcacaaga
    42001 atataaagaa tattatacga agggctatca aacagatatt gcttcaatca agatcagtga
    42061 gaaagagatg gcatttacgt ttaagaatgg aacaacgaaa cgttcaaatt atcgttatgt
    42121 agggaaagaa atcttgacct acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc
    42181 agaagataag gaaagtggcg cttttagata tattcaattc agtgatcaca cgattgcacc
    42241 ggctgactcc gaacattttc atttatatgc aggaaatgaa agtcaagaag tattgctgaa
    42301 agaaatggag aattggccaa cattttatcc agatacaatg gatggacgtg aaattgcaca
    42361 ggaaatgctt ttgcactgat aggcaaaaaa gaaccgaagg gtttggatcg gctggtcatt
    42421 tagtcggtcc aaactattcg gttcttttta tgaaatcaat ttctttaatc cacggataga
    42481 ggctgggatg gtctcgccgt ttgccaacgt gacttctctg gtttttttat taatcgaccg
    42541 cacattttgt gtattgataa cataggattt atgacaacgg ataaaattcc gatgcatttt
    42601 ctcgatttct ttgatcttct catagaattg taattgggca tgagtcaaat ggacttccag
    42661 tttatgtggc gctcctagcg aagtagaaaa atattggatg tctttgatcg gtaaaaattt
    42721 gatctcatca ttaaactcta ttttgaagat atctttttgg atttgggaca tttgaaaatc
    42781 ttcgaaaatt ttgctcagga tatgctcgac attttctttt aaatcataaa gatcttcttt
    42841 gacgatataa tcagtcggtt cgatgttgct ttttaagatt ttcatcgaca tttccgtatg
    42901 ggaagtcaca tagattattt ttcctaaagg atctaattca cggatcaatt taccgatcgc
    42961 tacaccatcc atttcactgt cttctagttc gatatctaaa aagtaaatac caaaagaaga
    43021 agttctgatc ttggctgcat tgagaagttc ttccggatca gaggtagaaa tatggacata
    43081 actgtctaag ttttcaatca tgattctatt tttgatcacc gttgataaaa cttctaattg
    43141 ttttggatcg tcttcacaaa tataaatggg aatcatatca ttactccttt attttatttg
    43201 tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg
    43261 attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt
    43321 ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt
    43381 gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt
    43441 ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag
    43501 gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa
    43561 ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt
    43621 catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa
    43681 tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg
    43741 aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt
    43801 ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc
    43861 taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata
    43921 tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt
    43981 ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg
    44041 gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc
    44101 agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac
    44161 cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt
    44221 caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa
    44281 agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac
    44341 ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat
    44401 gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca
    44461 taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa ttggcgtatt
    44521 atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata
    44581 caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct
    44641 ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca
    44701 agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag agaaaaaatt
    44761 agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg
    44821 attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag
    44881 accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat
    44941 aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca
    45001 tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc
    45061 accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag
    45121 acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct
    45181 tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta
    45241 tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg
    45301 tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt
    45361 tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa
    45421 attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca attgtttgaa
    45481 gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa aggaaaaata
    45541 gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt agaagacaat
    45601 gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac aatagaagaa
    45661 taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt tagctctgag
    45721 ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc cttgtgaccg
    45781 aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac
    45841 agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt agcttttaag
    45901 gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt
    45961 ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga
    46021 aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg
    46081 tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat
    46141 tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg
    46201 acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt
    46261 tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga
    46321 aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat
    46381 cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac
    46441 aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg
    46501 aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg
    46561 atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag
    46621 aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct
    46681 ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag
    46741 cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat
    46801 aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga
    46861 tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga
    46921 taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag
    46981 aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat
    47041 tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag
    47101 caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa
    47161 aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa
    47221 aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata
    47281 cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat
    47341 tgccagtttg agcagaggca aaagcaccat attggatata agtataactg tccatggctc
    47401 ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt
    47461 ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa
    47521 aaaggaaaaa ttttcagaaa actaaaatca tatcgcttcc cattttcatt tttagaaagg
    47581 tctgttagta taaagaaaaa atagacaacg aaactttaaa taaccaactt gaaataaaca
    47641 aattcaaaga agaaagatgg gaaggaaatc aatcagttga atgaaaaaga aattctccga
    47701 ttacaaaaag aattgaaacg ctgggagaac aagtataaaa agcttcaaca agtctttcgg
    47761 ctacaagaag aaatcattgc tacctatgaa gcggcttatg gcgaagcacc cggacttatt
    47821 gaggaagaag tacaagtaaa agaaagtgaa ggaaacagct ctttctttaa aatgatggag
    47881 aaacgttcct ataatcgctt caatcaagaa caaaaagcag ctatcacata tgatatgaag
    47941 aaacatcttc gaatcatagc aggagctggg agcggcaaaa cgcaaacaat ctgtgcaaaa
    48001 gctgtttatc tgatgacgca aaaacaagta gacgaagaac gcatactaat gatcactttt
    48061 acaaggaacg ctgcaaatga actaaaaaaa cgagtagata atttcagcca gagaaaaacc
    48121 gctgtccata tcggaacatt ccatagtatc ttttttagga tatacaatga aatttgtcga
    48181 aaatttcctg aagtagcaat gcaaggaata caaggagact tttcaaagga tactgcacaa
    48241 aaagtgaatg ctgttttgca acagctgata cgtaaatata atctttatat atttgatcaa
    48301 tatggagaaa aaacgattgc ttctcggtta gactattggc agaatatgaa cctttcagta
    48361 gaagaaatgg ttcagttagt taaagaaaaa tatgattcca ttgataagaa tgcacgacag
    48421 ccgatatcag aacgactgta tggattgctc acggaactcc aagaacaaaa aagaagccag
    48481 caattgctag agttcaatga tgtactccaa aatctgaaaa gcgctttgga gaatgaggag
    48541 atccagcatt atatcggcca aaaatatgat tatctattca ttgatgaatt tcaagatact
    48601 aatccacttc aatgggaaat cgtgaaacgg atgacaaagg aaaacaggat caagttgatt
    48661 gttgttggag atgatgatca gagtatctat ggattcagag gatcagagcc agcatacatc
    48721 aagaactttg aaaaagaata tcctaccaaa actcttttct tattaactaa ttatcgttct
    48781 cgggcagcta ttgtccaagg agcaaataga ttgatcagct ataataaaaa tgatcggata
    48841 ccaaaggcga tgattccagc acaaaaagaa gcaggtgtta tggaagccta tttgttttct
    48901 gatactaaag cagaaagtca gtggctaatc aaacaaattc agacatttat tcaaaaaaat
    48961 gggaaatata aggaatcaat catattatac cgatcagctt cacaaacgca gcagcttgtc
    49021 caatctctgc tgaaaacgaa tattcctttt gtactggaag cagatagtcc atatgaaggt
    49081 atctttggta tcaagtcttt ccaacatttc tatcaaaaaa ttgttcattg gcaaacagca
    49141 ctgaatctgc aaaagaaaaa tcaggcctac cagcaattgc tccggcagct gatggcagac
    49201 tgctacctga aaaaaagtga atgcgatcaa tacttttctc ctaaatatca aaatatagcc
    49261 gttgcagatt atattttgtc gataagcccc aatttaaaat cgaaatctgc agaaatccga
    49321 gagtttgagg aagcgttgcg taaaacagac cagcatcaag aaatctattc gcttgttcaa
    49381 agctatcttc atctgccacg tatagctaag gaaatcgacc agagtgatca agaatggata
    49441 cgagaagaag taaagcagca tgctactttc agaagactcc aagccttatc gcaagaaacc
    49501 caagcgacag ctaaagaatt aaaaacccgc ttgattgctt acagacaagg aaaacttgat
    49561 gcactatgta tccagagcat tcacaaaagt aaaggtctta gttaccgaaa tgtctttttg
    49621 attggttgta acgaaggaag tttgccttat aacggagcga cagaaaagaa agtaatcgac
    49681 tttaatgaaa taaaagcaga accagtaacg acgatagaag aagaacgaag actattttat
    49741 gttgcgatga ctagagcaag aaatcgtctg tatctatgtg tacctcaaat gaaaaattca
    49801 agaaaattga aagtatcgag atttatcaaa gaaaccggga gtcgtgtgaa aaaaggaaag
    49861 tgatagaaga gagggtgtga cagaagtggt cagtttcaag aaagaagaag gaatgtacga
    49921 aaattgcttc tcaaattttt gtgaaatttc agcttatttt cgaagaagct atttctggaa
    49981 cacagtttat tcggaaaagg gaggtgtga
//
LOCUS       DAZULG010000010        48094 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000010 DAZULG010000000
VERSION     DAZULG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.010"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..268
                     /locus_tag="ACG9CY_000477"
     CDS             95..268
                     /locus_tag="ACG9CY_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937017.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            complement(334..942)
                     /locus_tag="ACG9CY_000478"
     CDS             complement(334..942)
                     /locus_tag="ACG9CY_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS4937018.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(948..1976)
                     /gene="vanB"
                     /locus_tag="ACG9CY_000479"
     CDS             complement(948..1976)
                     /gene="vanB"
                     /locus_tag="ACG9CY_000479"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS4937019.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            complement(1969..2940)
                     /locus_tag="ACG9CY_000480"
     CDS             complement(1969..2940)
                     /locus_tag="ACG9CY_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS4937020.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            complement(2937..3764)
                     /gene="vanW-B"
                     /locus_tag="ACG9CY_000481"
     CDS             complement(2937..3764)
                     /gene="vanW-B"
                     /locus_tag="ACG9CY_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS4937021.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            complement(3782..4588)
                     /gene="vanY"
                     /locus_tag="ACG9CY_000482"
     CDS             complement(3782..4588)
                     /gene="vanY"
                     /locus_tag="ACG9CY_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS4937022.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            complement(4759..6102)
                     /gene="vanS-B"
                     /locus_tag="ACG9CY_000483"
     CDS             complement(4759..6102)
                     /gene="vanS-B"
                     /locus_tag="ACG9CY_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS4937023.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            complement(6105..6764)
                     /gene="vanR-B"
                     /locus_tag="ACG9CY_000484"
     CDS             complement(6105..6764)
                     /gene="vanR-B"
                     /locus_tag="ACG9CY_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFS4937024.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            complement(7382..7549)
                     /locus_tag="ACG9CY_000485"
     CDS             complement(7382..7549)
                     /locus_tag="ACG9CY_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937025.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            complement(7707..7892)
                     /locus_tag="ACG9CY_000486"
     CDS             complement(7707..7892)
                     /locus_tag="ACG9CY_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4937026.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            8037..8408
                     /locus_tag="ACG9CY_000487"
     CDS             8037..8408
                     /locus_tag="ACG9CY_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4937027.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            8666..8995
                     /gene="mobC"
                     /locus_tag="ACG9CY_000488"
     CDS             8666..8995
                     /gene="mobC"
                     /locus_tag="ACG9CY_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4937028.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            8956..10284
                     /locus_tag="ACG9CY_000489"
     CDS             8956..10284
                     /locus_tag="ACG9CY_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4937029.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            10281..10652
                     /locus_tag="ACG9CY_000490"
     CDS             10281..10652
                     /locus_tag="ACG9CY_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS4937030.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            10835..11071
                     /locus_tag="ACG9CY_000491"
     CDS             10835..11071
                     /locus_tag="ACG9CY_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4937031.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(11371..11574)
                     /locus_tag="ACG9CY_000492"
     CDS             complement(11371..11574)
                     /locus_tag="ACG9CY_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFS4937032.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(11578..12522)
                     /locus_tag="ACG9CY_000493"
     CDS             complement(11578..12522)
                     /locus_tag="ACG9CY_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937033.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            complement(12523..16443)
                     /locus_tag="ACG9CY_000494"
     CDS             complement(12523..16443)
                     /locus_tag="ACG9CY_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFS4937034.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            complement(16592..18673)
                     /locus_tag="ACG9CY_000495"
     CDS             complement(16592..18673)
                     /locus_tag="ACG9CY_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS4937035.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            complement(18670..19899)
                     /locus_tag="ACG9CY_000496"
     CDS             complement(18670..19899)
                     /locus_tag="ACG9CY_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFS4937036.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            complement(19889..20140)
                     /locus_tag="ACG9CY_000497"
     CDS             complement(19889..20140)
                     /locus_tag="ACG9CY_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFS4937037.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            complement(20163..22154)
                     /locus_tag="ACG9CY_000498"
     CDS             complement(20163..22154)
                     /locus_tag="ACG9CY_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4937038.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            complement(22186..24585)
                     /locus_tag="ACG9CY_000499"
     CDS             complement(22186..24585)
                     /locus_tag="ACG9CY_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFS4937039.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            complement(24515..24937)
                     /locus_tag="ACG9CY_000500"
     CDS             complement(24515..24937)
                     /locus_tag="ACG9CY_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFS4937040.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            complement(24951..25487)
                     /locus_tag="ACG9CY_000501"
     CDS             complement(24951..25487)
                     /locus_tag="ACG9CY_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFS4937041.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            complement(25524..26387)
                     /locus_tag="ACG9CY_000502"
     CDS             complement(25524..26387)
                     /locus_tag="ACG9CY_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFS4937042.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            complement(26479..26694)
                     /locus_tag="ACG9CY_000503"
     CDS             complement(26479..26694)
                     /locus_tag="ACG9CY_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFS4937043.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            complement(26771..28579)
                     /locus_tag="ACG9CY_000504"
     CDS             complement(26771..28579)
                     /locus_tag="ACG9CY_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS4937044.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            complement(28576..29052)
                     /locus_tag="ACG9CY_000505"
     CDS             complement(28576..29052)
                     /locus_tag="ACG9CY_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFS4937045.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            complement(29127..29339)
                     /locus_tag="ACG9CY_000506"
     CDS             complement(29127..29339)
                     /locus_tag="ACG9CY_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4937046.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            complement(29361..30326)
                     /locus_tag="ACG9CY_000507"
     CDS             complement(29361..30326)
                     /locus_tag="ACG9CY_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFS4937047.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            complement(30817..31338)
                     /locus_tag="ACG9CY_000508"
                     /pseudo
     CDS             complement(30817..31338)
                     /locus_tag="ACG9CY_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(31354..32052)
                     /locus_tag="ACG9CY_000509"
     CDS             complement(31354..32052)
                     /locus_tag="ACG9CY_000509"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4937048.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(32009..33670)
                     /locus_tag="ACG9CY_000510"
     CDS             complement(32009..33670)
                     /locus_tag="ACG9CY_000510"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS4937049.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            33888..35288
                     /locus_tag="ACG9CY_000511"
     CDS             33888..35288
                     /locus_tag="ACG9CY_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS4937050.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(35540..36619)
                     /locus_tag="ACG9CY_000512"
     CDS             complement(35540..36619)
                     /locus_tag="ACG9CY_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4937051.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(36705..37664)
                     /locus_tag="ACG9CY_000513"
     CDS             complement(36705..37664)
                     /locus_tag="ACG9CY_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4937052.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(37676..39187)
                     /locus_tag="ACG9CY_000514"
     CDS             complement(37676..39187)
                     /locus_tag="ACG9CY_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS4937053.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(39209..39772)
                     /locus_tag="ACG9CY_000515"
     CDS             complement(39209..39772)
                     /locus_tag="ACG9CY_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4937054.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(39778..40641)
                     /locus_tag="ACG9CY_000516"
     CDS             complement(39778..40641)
                     /locus_tag="ACG9CY_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4937055.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(40655..41551)
                     /locus_tag="ACG9CY_000517"
     CDS             complement(40655..41551)
                     /locus_tag="ACG9CY_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4937056.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(41726..43015)
                     /locus_tag="ACG9CY_000518"
     CDS             complement(41726..43015)
                     /locus_tag="ACG9CY_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4937057.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(42987..44504)
                     /locus_tag="ACG9CY_000519"
     CDS             complement(42987..44504)
                     /locus_tag="ACG9CY_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS4937058.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            44702..45592
                     /locus_tag="ACG9CY_000520"
     CDS             44702..45592
                     /locus_tag="ACG9CY_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS4937059.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(45665..46489)
                     /gene="nadE"
                     /locus_tag="ACG9CY_000521"
     CDS             complement(45665..46489)
                     /gene="nadE"
                     /locus_tag="ACG9CY_000521"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS4937060.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(46501..47973)
                     /locus_tag="ACG9CY_000522"
     CDS             complement(46501..47973)
                     /locus_tag="ACG9CY_000522"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS4937061.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        11694 a        12077 c        10894 g        13429 t
ORIGIN      
        1 gaggggcaag gctttttttg agtggtggaa gtggattaga gcatggcaat agcaaaacag
       61 catagcggca tcctcctatg ggccgccaca ccttttgctg ccagaaaaga acggcggcct
      121 tgatttctca aaaccgccgc cagcaaaata caaaatgggc gtgggaaagg tctgcccaac
      181 agcggttttt ttcttttctg ccgctgggca gattgaatac tataatttgc aatgtattac
      241 aaaagatgct ttaaccactt tccagtaaaa gattgtccat gaccagcata gattttatca
      301 aatccaaaat ctgatacaaa accaatactt tcattatgaa acggcaaaat taaaataggt
      361 atcggggtat ggctcatcaa tcaacttata gtgccaccat tcaaagcgat aagactgaaa
      421 tccgctgctt tccatgattt tacgcaagca tcttcgattt tgtgcctctg catcccctat
      481 cccttttgct gtatgatgcg accgttcgtc cataaaatcg aagttacttc ccattgaaac
      541 aagttcccct gtatccaggt ggtagagcgt aagatcaatt gcgcttccac ggctatggct
      601 ggattgtgag gccacataac cctttgtaat caactcggct cgctctatat tggggtaaaa
      661 tttttctttt gtgagattgt cctccggttg cgctgcccaa cgcaggaagc agtccaccgc
      721 agttcttggc cgatatccat cccataaaag caagccatag ccaagcgcag ccgcatggat
      781 ttgtgcctcg cgcagagcaa acgccacggc ctttgtgccg atgatgcgat tcacctcata
      841 cccatccacc ggttttcctg tgaagttatc ccatgtggcg tacttggcat cccaacggac
      901 gccatgcaac atttcatcta aaaacaaaaa accattttcc atacttatca cctctttaac
      961 gccaatgtaa tcaggctgtc aatcagtgca ggaagcgtga ttcctgcggc ggccatcata
     1021 cgtgggtagc ggctgtacga tgtaaaaccg ggcagggtat tgacctcatt tagaacgatg
     1081 ccgccatcct cctgcaaaaa aagatcaaca cgggcaagcc ctctgcatcc aagcacccga
     1141 tatactttct ttgccgtttc ctgcacccga tttcgttcct cgaccggaat gtctgcggga
     1201 actgtaatca tcgcattttc tgagcctttt tccggctcgt tttcctgatg gatgcggaag
     1261 gtaccgtggc tcagccggat ttgatccact tcgccgacaa tcaaatcatc ctcgttcccc
     1321 atgaccgcac acccgacctc acagcccgaa atcgcttgct caattaagat ttttccatca
     1381 tattgtcctg ccgcttctat cgcagcgtta agttcttccg taccgtttac tttggttacg
     1441 ccaaaggacg aacctgaccg tgccggcttc acaaagacag ggtaggtaag cgcacccgcc
     1501 tccggcttgt cacctttatc aatcatttga aattcgggaa cggcgatgcc cgcatttttt
     1561 gtaagaatgt aggccagtga tttgtccatg caagctgcgg agctttgaat atcacagccc
     1621 acatagggga taccagacaa tacaaacagc ccctgtatcg caccatcctc cccgcatttg
     1681 ccatgcaaaa ccgggaaagc cacatcaata cgccgtgttt cgtattcgct ttctttcatg
     1741 acaagcagcc catgcgtttt cctatccggg gagagtatgg cggggagact gtcggcttcc
     1801 cattccgtac atggcttctt gcatagcttc catacaccgt tttttgtaat tccgatgtag
     1861 tgcggatcga atttttccgt atcaatgttc gcagcaattt ctattgcgga ttttaccgac
     1921 acatcatgtt cctccgagca accgccgaag atgattgcga cttttattct attcatgctg
     1981 taaactcctt tcaaaattga gacaattcct gattgttttt tctgtagtat cctgcagcac
     2041 acgctcagtg tagtacgccg tatggggtgt gatgatcaca ttcggcatcc ttaaaagctg
     2101 cgacaaaaag ggatggtcaa gcactttctg cgagcaatcg gtataaacaa actgatcctc
     2161 gccctccaac acatccagtg ccgcaccacc cagctttccg ctttccaacg cctccaccag
     2221 cgccccggta tcgacaagcg ctccgcgccc ggtgttgatt aaaaatgcgc cttgcttcat
     2281 ctctccaatt tgcttctgac cgatcagatg gcgggtatcc gcacaaagcg gcacatggag
     2341 cgtaacaata tcgctgtttt ttagaagctc atcaagctgg acataatctg catccatttt
     2401 tcggctgtta tcataggcca gcacatggca tccaaatccc cgcagccttt tgacgaccgc
     2461 ttgcccgata tggccggttc caataactcc cacagtcata tcccccagtt ctttcccccg
     2521 gatacgatcc aatctgaaat cttgttgcgc cacggcgtgc atggtgggtt ttgcaccccg
     2581 tatggccatc agcatcagca tcaaagcata atccgcaacg ctgtccggcg aatacgcaac
     2641 cgtgccaacc gagattccca ttctctcggc ggcagtcgta tcaatgtgat cgcagccgat
     2701 gctgcgggta gaaatgtatt ttaccccgac ctttctcagc gcaagaattg tcgcctcgga
     2761 aacctcggac ttatggccta cgctaacgca ttgattgcca gcggccaatt ttgcgttgtc
     2821 tgccgatatc gcatcgctaa tcagcgtagg gataatatga aaatccggcg ataaagcgcg
     2881 gaaagcattt gcctcatcct gttcgcatcc aaaaacagta atgcccacac tttttctcat
     2941 tggttctcct cctgaatatc cacactttcc gggaggggat aacagatttt gcatttgttt
     3001 gtataaagca gcttgcaatc tattatttcc ccggtaccta aggcaacttg catccgttta
     3061 acctgggcat attcataaat cccgccactt tcacggacat actggatact gccatttgta
     3121 attttgtata atgcttgggg ctctttgtcg gcggacacct gaccgatgat tttctcatca
     3181 tctaaggtca cccatatttg gtaggtgcag tcggtatcgt ttcgcacttt taaatcaatc
     3241 cagccctctg aaatggttgc atccacccct ttaatctcgt cactgtttgg ctctggaaac
     3301 tcctttactt cgtgacctct gcgctggata attgtcaatg gcgtatgcag gaacatccaa
     3361 aatagtaaat tgctcatctg gcacataccg ccgcccgaca tggtggtgag tttaccattg
     3421 gtcaccgtaa ggccgtcttt atagggggta tctttgtccg catggcgtac cagccaccag
     3481 aaagaaaagg gttcccccgg tcttatcaat aagccgttta aggtctttgc tgccagcttt
     3541 aagttgaaca ccttgttttc ctggtatatc atgtcaaatc cggtattgtg gttgtataat
     3601 gcgcaatccg tttcaaagag caaatgggaa agcgattttt cccctatcgt ctgcgcatag
     3661 cgacagccgt caaatctcat tcccgcataa aagcacattt ttctctgcgc ccgtctcatt
     3721 ggaagcagga acgggaaacg ctgtgtcaat ctttttctgt ccataacata acatcctttt
     3781 ctcatttttc tggttttaaa tattcctcaa ggcaaacccc ttggttgtac atctctgtgg
     3841 cagcttcgat gccaacatat cggtaatgcc acggctcgtt gctcacaccg gttatctctg
     3901 tcttgtctgg tgggtagcga cgaataaaac caaaacgata gctgttttca tccagccatc
     3961 tataaacctc gttgcctgtt gaatgaattc cgtccgcatt gatatccaca gcaagaccaa
     4021 gctgatgctc gctcgttccc ggcacggcca cccaagtttc tgcttccgct ttagcctgtg
     4081 cagaggtgta gcctttcgcc ttatattcgg caattttttc atccataatt tcttgctgtt
     4141 tttctgttgt tcggtatccg gatgcgacaa tcgggtaaac tccatcagtt cttgcggcat
     4201 caaacaaatc ttgaagatag ggagaaatcc gaatatctat ccgctcacca tttgatagtt
     4261 gctcaagctc cacatcgtac tgtgcgggga tggggttctg cctgttcact aaaatcaggc
     4321 tccattcctg ttcatctccc tgttctttgg cggtggcaag agcgttttcc tccaaattct
     4381 ccactacgtc agtgctgctg cctgtatgag ttgcagggat gggcggtatc tgtgttgcct
     4441 ccaatacgga agccagtctc cgccccaaaa acagcgtgca gactgtgaac gaaataatga
     4501 acgcacatag aaaagcccgc ttttccacgg attgtttcat atgccgtttg tgatgatccg
     4561 cattggaatg atagttgctt ttttccattt gatcgaatgc ctcctttgca ataaaactac
     4621 gatttgtggc tggtacgatt gttattatgg caagcgcatc tgtactttcc ctgtagccag
     4681 tctgtagttt tcctgtagca gcaaaaaaag accggcaggg ttatacctgt cggtcaaaat
     4741 catttaagtt ttaaatattt acaatgttga tgtgagcggt aaatccagcc agaacaaaac
     4801 gccatccgag gtgttttcca gcgcatactg gaggctcatg gcatccagcg ttttttgtac
     4861 gatggcaagt cctaaaccgc ttcgcccact ttttctgctt tgcgcctgat caatgcgata
     4921 gaatggggtg aacagccttg gaagcgcagt atcatcaatg tgaacgccca tgttcaaaac
     4981 aaaaaggcgg catttttcag caccaggctc actccatatc cgtacctcgc ctccctgcgg
     5041 cgtgttctga actgcattca atatgacatt ggatagtgcc ttttggagca gcctcggatc
     5101 ggacaggaca atttgcccgg ctggaatatc tgtgacgaac cgctggttgt ttgcctctgc
     5161 caaggtttga aaatcgggca gcaactcggc aaccgtgcgc cctatgtcca acggttcagc
     5221 tatgggtacg attctcccat cattcaggct gacaagctcc agtatttcgg aaatgatttt
     5281 gccctgccta tccatcattt tgatgcattc gcgcagatac ttagaatgat ctttgtagtc
     5341 accgatattt tcaagcattc cctccaacag aacgcttgta gccgcgatgg gcgtttttag
     5401 ctcatgagaa gctgccgcaa agaaatatcg ctgtgtttcc tccaactcat gttcccttgc
     5461 gatttcatcc tccagccttg cgatggtttc tttcagcctg acatacatgg aatgcatatc
     5521 atgagccagt gcgccaagct catcctttcg ctccagcggc ggcggtacat ctttcaggtt
     5581 tgccatttta ttcgcactgt ccgctaaggc tttgatcggc gttgtcattt gccgcgcaaa
     5641 gatatacgcg cataaaaggc tgaatacaac cattatcgca atcataacaa ttccccgaat
     5701 tgtcagccct tgataaagca atcccacacc tgccttgctt tgagcaacaa tcgaaatatt
     5761 atcatctcta tgtaccacat aaagaaagtc gggtctaaaa ctatttgatg tattggcatt
     5821 tggtgtggca tagagtacgc ttccctcttt atcttcaata taaaactcaa aggattggtt
     5881 attgtagtga aacagccctg ccacctcttg aatatcaagc ctatcgctat tctgaatcag
     5941 ttccaccaat ggctgatagg attttactgt ttgctgtaat tccatcactc tgaaataaga
     6001 cacaaattgc tgtgcaaaca gtgttgctgt tacaccgaca agcagtagca gggcaatgat
     6061 cgtataggaa aaaaccttaa tgaaaatccc ttttctttcc attattatga ttcctccaat
     6121 cggtatccta caccgcgaat ggttttgatg atattttccg gcagcttggc ccgcaaattt
     6181 ttgatatgag tgtggactgt gctgccatca ccctcaaagt catatcccca tatacgtgac
     6241 aaaatgattt catgcgttaa ggttctgcct ttgttctgca ccagcagcaa aaggatttca
     6301 aattcttttc gtgtcagggg cagctctgta ccgtcacaaa gtaccgtgaa atcctccggc
     6361 agaagtgtca gcctgccgac acggatttcc tttgccagcg caccgctgcg ccgcagcagg
     6421 gcttccaccc gctttagtaa aatctgcatc ttgaatggct ttgttacata gtcgtctgcc
     6481 tctgcatcaa acgcccggat ttggttttcg tcatccgaca gggctgtcat catcagaatg
     6541 ggagtatcat ttttcgcacg aaattcacgc aaaagttcat gcccgttcat accgggcagc
     6601 ataatatcaa gaataaccag ttgataagtg ttttcgtaaa acttggtgta tgcctcattt
     6661 ccatctgtgc aggcatccac ctgatatcct gcctcagcca gaaacgccct tactgtatta
     6721 cagatatgat catcatcctc gacaagtaga attcgtatcg acataaaaat ccctccattt
     6781 taagaagata acataacagt ctgtagtttc cctgtagttt caaagcgatt tcttttcgga
     6841 ttttaatcat ccgtcattgg gatactcgct tcttttcatg gtgtatgtat ctatattatt
     6901 ggtttaaacg aattaaatca agataataat aattaacacc ttcttaatgt ttgggatgtt
     6961 tcgattacag cggttgtcaa aacatcccga taatactggc aagcaatgga tcagaatatc
     7021 ctttacggat attctgatcc accacttgcc cgcctgcggc gttctcgttt tgcttgttgg
     7081 ggacacccca agcctcagca aaatgcgcgg cctctttttt gcgaatgcac cttgcggtat
     7141 ggcttttcac acaggcaggg cctgtgtgta ctccctccgg tcgctcttgc cgcactccgc
     7201 gcagcagatt cagtcctaat accgttcaag acagtagaaa atcagcaagt ctatattcct
     7261 catatcttca tcatccgtgt ccttgcagtt acaggaaatg taataatcgc caaatgtaaa
     7321 tcccagaata ttttcctcaa aaagccgtac ctccccggta ttaaaatcaa tcaccccata
     7381 ctcaaaggcg cattagacca gcagttatcc aatgtctgga gacttcacca taaagaatcc
     7441 agatgccggc cttttcaatt ctaccatata cggctgatac agcagcagct tcctcgtctt
     7501 tgtttcgggc attttccatt tctgtcatga taaagtttcg cttcttcata catccatgtg
     7561 taaatctttt ctttctgttt cagatgagaa tactttttat cggtctggag cagcctgcca
     7621 tctatcatct tatagttttt ccgtgacaaa ataccatgct ttctctctct tcaaaaccat
     7681 atccaaaatg cgccggtttc attatactat tcagtaaccg cctttccttt ctttgtacgc
     7741 ctgtcaatcg gcttttccgc agttttggga atgagccaga tcgtcgccat ctttacagcg
     7801 ccggggatgc gtcccgcagc acaatagtag tttatccatc gtgggggcac accccatttc
     7861 tcgcctgcct ctttcaacgt catgtagtcc atagtacacc tccacagggt acattataat
     7921 tcttttgctc gaagaataaa agaaacaaaa tgtgaaatat aaactttaag aaaggtgcat
     7981 ataccaacat tccacgggca gagtatgaaa tatacaatgt aagtgggagg tgcgatatgg
     8041 cgaatattga agattgtccc ggctttgaaa ctttcggcgc ggatgtgaaa gcggcccggc
     8101 aagcaaagcg cgtttcgcgc aaagcaatgg cagagaaaat aaatattgac tggcgctatc
     8161 ttgccaacat cgaaaacgac ggggccattc ccagtctgcc ggttgtcata cagcttatta
     8221 aagcctgcgg gcttcctgtg gaacgctact ttaacccgga gatcatgcgg gaggaaagcg
     8281 aactgcggca aagggtcagc cacaagctga aactctgccc cgaagaatac ctgccgatca
     8341 tcgagggcgc gattgacagc gccatactaa ttgaaaagcc gaagaagaat gaaacggagg
     8401 atgtgtaact ggcatcctct gttttacttt tagcgggagc tgcacagcgt agcttctatt
     8461 tttttgcact tttttctttc cagagggcta ttttgcgggt agtttggggc tttggaaacg
     8521 tcttgttagt ggagaaacag taacgtagca agcataggga gtgtagccac tcccggaaaa
     8581 acggcctgcc ccgtccctgc cgggccgcgt ccgttttgct tgttggggaa atccccaaac
     8641 ccctgaatgg aaaggacggt gaagcatggc aaacagaaag cgaaaaatcg tgctgcgcgt
     8701 ccctgtgacg ccggaggaac gggctatgat tgaacagaaa atggcgctgc tgcatacgaa
     8761 gaacttttca gcctacgccc ggaagatgct cattgacggc tatatcgtca acatggacac
     8821 cagcgacatc cgggcgcaga ccgccgagat acagaagatc ggcgtcaacg tcaatcagat
     8881 tgcaaagagg ctgaacggta tggggccggt gtacgcacag gacatcgagg atataaaggg
     8941 ggcgctggcg cagatatggc agttacaaag atacatccta tcaagtcaac gctgaaaaaa
     9001 gcccttgact acatcgagaa cccggacaaa actgacggca agctgctggt ggcctccttt
     9061 gcctgctctt atgaaacagc ggacattgaa tttgaaatgc tgctggcgca ggcataccaa
     9121 aagggcaata atctgggcca ccacctgata caatcctttg cccccggcga agccacgccg
     9181 gaacaggccc atgagatcgg gaagcagctt gccgaggaag tgctgcaagg gaaatatccc
     9241 tatgtgctga ctacccatat tgataagggc cacgtccaca atcacattat tttctgtgcc
     9301 gtggatatgg taaatcagcg caagtacatt tccaacaaac agagctatgc ctatatccgg
     9361 cgcaccagcg acaggctgtg caaggaaaac ggattgtccg tggtgaagcc cggccagagt
     9421 aagggcaaga gctatgccga gtgggacgcc cagcggaaag ggacgagctg gaaagcgaag
     9481 ctcaaagcag ccattgacgc cgctatcccg caggcaaaag attttgatga tttcctgcgg
     9541 ctcatgcagg cgcaaggcta tgagatcaag ccgggaaagt ttatttcttt ccgcgctccc
     9601 ggacaggaac gctttacccg ctgcaagaca ttgggggaag cctacaccga ggaagccatc
     9661 aaagagcgta tcaaaggccg cgttatcacc agagcgccca aagagcgcaa ggggatttct
     9721 ttgcggattg atctggaaaa caacattaag gcccagcagt cggcaggcta tgagaggtgg
     9781 gcgaagctcc acaatttgaa gcaggccgca aagactttga atttcctgac tgaacatggg
     9841 atagacacct acccggattt ggaaagcaaa gtggccgaga tcaccgccgc cagcgacgag
     9901 gccgccgcta ccctcaaagc tatggaacac cggcttgcgg atatggcggt gctgataaag
     9961 aatatttcta cctacaagca gctctggccc gtggctatgg aataccgcaa tgcagcggat
    10021 aaggcaaaat tccggcgtga gcatgaaagc acgttgattt tgtatgaagc tgcggcaaag
    10081 gccctaaagg gacagggtgt gaaaaagctc cccgatctgt acgccctgaa agccgagtac
    10141 aagcgattgg ccgaggaaaa ggaacggctg tatgagaagt acggcgaagc gaaaaagcag
    10201 atgcaggaat acggcatcat caagcagaat gtggacggca ttttgcgtat gacgccggga
    10261 aaggaatgga cgcaagagct atgagtattc cacgaatgaa ttaccctcaa tatcgcaagg
    10321 cccggaagct gacgcatgag tgctgcaact attgtgacgg caactgcctg ttgctggacg
    10381 acggcgaaga atgtatttgc gtacagagca tttcctattc gcttctgtgc cgctggttta
    10441 aggccgctgt cctcccgctg gatgcggccc tgtatgccga gatcaccaaa gaccgggacg
    10501 aagtgaaacg ctgctgtgag tgcggggcca cgttcacgcc gaagtctaac cgggccaaat
    10561 actgcgcggc ctgctccaaa cggatgcgcc gccgcaaaga ggccgagcgc cagcggaaaa
    10621 gatactggaa acctacgcac ttagagctgt aaaagccctt atatttcaag gcttttccgg
    10681 catggtcagc ggggtatgtg ggtgttttat cctatccccg ccgaagtgct gttctaaatg
    10741 cgtagaattt accagcagaa aagcgccgtt ttctctggtt ccatgcttcc gaatatggta
    10801 tcatagaaaa aatcagtctg acgggggtat caggatggac ggacaagaaa agctgctgga
    10861 ctatgaaacg atcaaagccg ccgttgccgg tgagaggtgg gccacggaaa aagtgttggc
    10921 ccattatgct gactacatca acgagctttc cacggtggag atcagacagg caaacggcaa
    10981 aaagaagaaa gtcattgacg aagatatacg gcagcgcatt tccctgaaac tgctggaagc
    11041 attgccgagc ttcccgctgg aacagagata gacggcaaga gggctgcacc ctcttacgcc
    11101 gctttgcggc tatcacctgc acacccctga aacagataca cccgctgcgc ggctatatct
    11161 gtttcagggg tctaattata gctgcaacct acgcatttaa gggattgaaa accgcccgct
    11221 gcaaggcttc tcccgcatgt ttgcaggggc aagcgaatac ttcatcctat ccccgacaat
    11281 accgggttct aaatgcgtag gaataggcaa aaagaaaaga cgccggacgc aagcgaccac
    11341 atggccgccg cgcccggcgt ctgctgttcg ttattcgtct gtgaagtgga acttctggcc
    11401 gtcatagtcg gcatccgtca tgcgttccag cttggaaagg gtctgctggg cctgcgcccg
    11461 catatcccca tccataacca gcatgacggc ggtgagcctg tttatcgttc tgcgccggtc
    11521 tgcattgtgg tacatacaga tcaggttttc ttcctccacg gtaaagtgca tcattcctta
    11581 tacctccaat tcattctttt tggttctggc cgccgccttt tttgggctgg cggcctgttt
    11641 gctgtcggat agctgcttgc ggatagaggg ccggggcctg cgaatactca catcccggcg
    11701 ctcgtcccgg ttaatcacgg catacacgcc atgcctgcct ttcatcccgg taaaacacag
    11761 ggatttatag ggcagcatgg caaagagccg gtcattatcc ttggtggaag caatcgccgc
    11821 aaagtgcggg gagatctcca ccatgaaatg cgttttattg gggctgttag gcgcggacag
    11881 atctttcagg ctgtccacaa tacgccgggc ctcggtttca atccggctgt catgcaaagc
    11941 ggtgacgtgg actttgaaat cgccggggac ggcccgcccc cggctgtgct gctccgtccg
    12001 tagcgcctcc tgcagcacag ccgggtcatg gggctgggcc tcatagtaca aaaagtttcc
    12061 caatacccgg tcagggcttc cgtcaaaacg ctggctctcc gggatgtcac gggtgccatg
    12121 ctcataaaag agccggttca cactcaccgg ctgggcggtt tcaatgacgt gccggaaatg
    12181 ctgcggatag tccagctcat agagattgcc ccggatagtg ccgccctgtg tgccggtcag
    12241 ctccaccgcg taggccagta tcttgtcacg ggtctgctcc ccgtaaaatt tccatgtgtt
    12301 gtactgtcgg gtgtctttca ggaaaacatc acgttcccgg aaacagtacg tcccggaggg
    12361 ccgggacatc cagagcaggg tcttgtctgc ggcctgatcg ctggcggcat cccgcctgat
    12421 aatgtctttg tcaatgccaa agtcactttg ataaaacgcg gtgttctgcc gcatgatctg
    12481 ttccagcgcg gccagaacgt ccacattttc aaacttctgc atttactcta cctccaatcc
    12541 ctttgatttt tcccgcgccg gtggctgctt ggcctgttcg gtcttggccg cgttgagctg
    12601 cgcccggata gagggctttt ctttctccct gcgggccgca agctgtaagg cgctgggctt
    12661 ttcgccggaa aggggctgga tgcacttgat acggtcagcg acgcggattg tggtgtcgtt
    12721 taactgccgc tgccatgccc cggccttggg cgaccagcgg aaaccgttgc ttttcagctc
    12781 gtcgcgtaca gcagcatccg gcttcccctc aaagaagatt tgcaggcggt tggccacccg
    12841 gttgatctcc acagtgccgc cgtcaaactc ccatcccaca taggccgcct ccttttggcg
    12901 ggtcaattcc tcaatgcggc cctttaaccg gcgtatctcg gcgttattgt tggagagctg
    12961 ccaggactgg aacggttttt tctcatagtg ccagcccgac gccatatccg ctttcaggtt
    13021 ttcaatgcct ctttcggtca gatgggggca tccgtccagc gtcccatgct tgcggtagta
    13081 ggcattgacg gccttcatca actcctgcga cgcttccagc ttttccagtt tcttttccag
    13141 cttggaaacg gcctgcggat cgtcggcgct gataccaccc atgccggtgc tgcggatttt
    13201 gtcaagcaag ccctgaatgt actgaaattc ctccatgttg cggtcactgg cggcgttctg
    13261 cttttctttc gcccgtaccg ggaaattgga cggcccggca atcaggacag aggggacgcg
    13321 ggcggtaatc tcatagccct tattcatgtt ctcggccagc ttgcgggcgt aggtgtcaag
    13381 gaggctgtct atcttttcgt gatactctgg ctccacacgc tttttctggc gctccgcaat
    13441 ttccacggcc tgatcgacat agtgccggta ttccgccgtg gcgctgccgg gccggtagtc
    13501 agaaaagctc atggcttcct tggcgcggcg ggcggctccc tcattgatgg gataatagcg
    13561 gacagccgtt tgcgggggcg cagcctccgg gggttcctcc tgtgtctggc cggaagcaac
    13621 tgccacatcc tcaaaagata gctgttcggg ttcctccggc tgcggttcgg gcggcgtatt
    13681 gttgataatg ccgtcaatca tgttttcgtt ctgctccgta gacagttcgg cggtacgcag
    13741 gggattttcc cgcaaaaact ccggcacttc catatagccg aagctgtcta catagtgggc
    13801 ggtgtcctgt ccattctggt gcagcaccac cacgtcggaa acggaaaggc tgtgtcccct
    13861 aaaatctgcc gggtggtcga tgttgaatgt gcggtaaata tcctccaaag acgtgctttt
    13921 atccagcggt gccgtataaa caaggtcata gttggcactg tccacggcca gccctgcggc
    13981 ctgtaaacgc tcgtatggct caaaccgata gtcgcgggtt tcctccccgc ctcgaagctg
    14041 gtagatggaa aaggtgtcct gttggggtgc ttcatagcca gccgccgcgt attcctccac
    14101 ggtcataccg gctgcggcgg cctcctgcgc gatctcggcc tgtacctgtg ctttgtagtc
    14161 ctgcaagctc tggtcgaagt cagccagttt cggcggctcc ggcaactgat actggccctg
    14221 attgagcatg gggcggcact cctgcacata agagagaagc gcccccaaat aggccgtttc
    14281 ctccggcgtc agggtttccc cggactgtaa gcggccctgc gccgtctgtt ccatttgggc
    14341 aaggctgcaa tgcagcttca tataggggac aaactcgcct aacagcatat cccgatgggc
    14401 cttgtcctgc tcccagccct ctatgccggt tgtagcgatt acatgctttt tccagttttc
    14461 gtcctgcgcg taaaattggt ggtagctctg gatatgttcg atcagggaac cgtccctatc
    14521 tccaaaatcc tgccgcccct cgtagctgtc cggctcgccc tgaaagatac agtcaatgcg
    14581 gaacgcggtt ttgtcatagc ctgcctctgc cggatatgct gcatccagac gggcaaacag
    14641 cgcgtcggcc tcatgcaacg gcatttgctg gccgtccttt agctgggggc tttcgctcca
    14701 tagcacagtc acaagcggct cggctgccgg atcaagggcc ggggcctgtt cttcctcggc
    14761 ctgcgccggg gccgctgcct ccggggtaac gtcaaccgtg acggcctcct gttcctgccg
    14821 ggcctcgcgc tcctgctgaa gctgggcaaa atggccgtcg atctcggtta tcaattcgct
    14881 ggccgtggcc cggatggttt caagggaggc tttcagctcg gataactccc tgccgctgct
    14941 ccaatcggcc acatagccaa aagaatagtc cgacgtgtcc agcccgtaat actggcagac
    15001 ggtataggcc acgctttcgg cctgtacctc gcgggtgctg cggtctggcc gggccgcctg
    15061 ttcctcggcg ggggcctccc ggtcaatggc gtggagcttg gcatgggcaa tctcatggat
    15121 ggcggtcttt agggtctgca attcgctcat gccctcgttg atggcaatgc gctgttttgc
    15181 gtagaagcag cggcccttgg ccccgtctga aatcggctca atgcttatgg gatagggtga
    15241 tgtccgttcc agcgcggcaa agaaatcctg atactgctgc acgtcgccgg taagttcact
    15301 gatatagggc atgggaaaat ccttgccctc ggtctggctg atgtcaaaga ctgtcaccac
    15361 cttaaaggcc ggaaccgtca cttcctgttc ttcggtcacg ggcttgccgt ccgcgccgat
    15421 cacgggctgc tgggtggccg ggtcgatttt ctccatctgc tgcttaacct tgaacggggc
    15481 cggggccagt atcttaatgc ctttctcgcc ccgcttcaca tggcggtcaa attcatcctg
    15541 ccacctgtta tagcccgcca caatcgaacc gccctgcatg gcgatcagca gcgtattgtt
    15601 cagactgtaa tcgtgcatct tggacatagc ggataagtaa gccatatagc ggctactttc
    15661 aaaaagctcc tgtacgccgg tttccagacg gtcagtaatc actttcatgc gttctttgct
    15721 gttcatgccg tccagcatga tcgggataac cgggggcggc gctttgtcgg ggctttcgga
    15781 tgcctccggc tgctgggcct gcggttcctc ccggtcagct tccggctggg ggtagtgcat
    15841 cacccggtat tcctccggca ccggctggcc ctcatggacg cgctgccatt cgtcgccgct
    15901 tctcaccaga tagccgtact gggtaaaaac gccttgttcc tgctgggcaa tgtggcgtcc
    15961 aaactgcgcc gtatcaatcc cggctttcca ttcctccggc atatccacca tgccggaacg
    16021 gttcaggtag tagtcgccca actgtccatc ccccctaacg tcgggaagat gcacatagta
    16081 atccacattt tcggtgtagt cgatgatctg gccgatattc tcaaagccgc cacggggatt
    16141 ttgcagggcg gcgttcagct ctgcgatctc ggcggcgtca agctggccca gccgggcggc
    16201 aaggaaattc agctcgtcca cgtcggcggc cagcaccatg tcatagggaa tggcaaggtg
    16261 tctgtcctcc gggcaggaat acccgttgat aaaaaaatcc tgcggattgt cggcagtaat
    16321 gccaacatcc cgcagggcgg cttgcagcgt ttcggttgtg gtgggcaaat ccagccagac
    16381 gccttgtttg ccctgttcca aaaactgccg gttatggact tggatagaaa atacttcgtt
    16441 cacgcggtca ctcctttctc tgttctgttt caaaaaatag ggggttgggg acttccccaa
    16501 caagcaaaaa tccccggccc ggcagggacg cggccggggg attttaccgg gagtgtagcc
    16561 acactcccta tgcttgctgt gttactgtcc tttagctcct gtgttcaatg cggtagttga
    16621 cgtacttgcc cccggtatca gccaaaagca ccgtcccgtc gtaggttttg ccggtcttta
    16681 cggaatagag gccctttacc ttgactttgc cggttttgag cagcgcagcg gcgatcttcg
    16741 gggtgaacgc tttcttgcgt tcctcaaaaa atcggtcatt cttccacatg acgaactggc
    16801 aggcccggtt tccgcagtag tagtttttct tgccctcata gacagcctca ccacagcggg
    16861 gacacgtccc gataggaaca cgctcggcct gaaacaattt ctgcttttcc tcactgatac
    16921 aggaataggt ctgcaccagc tcccgcgcct gcgtctcgat gccgtccata aattcgtcag
    16981 gatcggcctg ccccttggcg atctcggtca gccggttttc ccattcggca gtaagctggg
    17041 gagaagtaag cgcctccggc agcaccacag ccagattgat gccgtccttg gtgggaatga
    17101 gctgcttgcc ttttctctgg acaaagccca tctgcaccag cttttccaga atggcggcgc
    17161 gggtggccgg tgtgcctaat cccttgcgct cggcatcctc cggcatatcc tcggccccgg
    17221 cccgttccat agcggacaaa agcgtatctt cggtgtaggg cttgggcggc gtggtgaaat
    17281 gctcggtgac agaggcggcc acgtctgcaa aggtctggcc ctcgcaaagc tccggcagct
    17341 cccgcaccgc ctcggcgtcc tcgtcggcgt cggtctttaa ggaagaacgg aaccggcgtt
    17401 caatctcttt ccagccgggg gaaaggacgg tcttgccctt ggcggtaaac tcctgcccgg
    17461 cacaggtaaa tgtggcggtg acggcctcat agatatgcgg cccggccacg gcacataaga
    17521 gcttgcagca gatcagggac agcagctttt tttcactctc ggccagagtg ccaaagccct
    17581 gcttcacaaa ttcagcggtg gggataatgg cgtggtggtc ggacaccttt ttgctgttca
    17641 ggatgcggtc aaactgcggg gaaaggttca ggcccttggc aaacggcacc agctcccaca
    17701 ggccggacac aaggctttcc acggcgggca gcatatcgtc ggtaagatat tgactatccg
    17761 ttctcggata ggtcagcagc ttctttagat acagtgcctg ggcatagtca agggtctgct
    17821 tggcggtata gccgaaaagc cggttagcct cccgctgcaa ggtggtaagg tcatagagct
    17881 tgggcggctg ttccgtcttt ttctcccgcg tcacggaaac gcaaacggcc tgcgcttttg
    17941 cacaagccgc cttgacggtg tttgcctcgg cagcgtcgga aaagcggctg cttaccgcgt
    18001 ccgcgccgcc cgccgtgata tggacgatgt gatatttctc tttcttgaaa tcggtaatct
    18061 tggcgtccct gtccaccagc atttttaagg tcggcgtctg gacgcggccc acagtcaggt
    18121 tcttgtggta caggacggaa aaaagccggg tggcgttaat gccgatcagc cagtcggcct
    18181 tggcccggca cagggccgat tgatacagcg gatcatagtc gccgcccggt ttcaggtggg
    18241 caaagccgtc cttaatagca ctgtcctcca tagaggaaat ccaaaggcgc tcaaagggct
    18301 tttggcatcc cgccatctgg tagacgaaac ggaaaatcaa ttccccctcg cgcccggcgt
    18361 cggtggcgca cacaaggccg gtcacgtcgg ggcgctgcat cagcgaacag agaacgtcaa
    18421 actgctgttt cttgtcgtcg gggacgatgt actgccatgt ttccgggaga atgggcaaat
    18481 cctcatagcg ccattttttg aaacgctcgt catagcgtcc ggcgtcggca aaggacacca
    18541 gatgtccgat gcaccagctc accagatagc cgttcccctc caaatagccg tcgtgtttct
    18601 ccgtggcccc gatcacgccc gcaatgctgc gggccacgct gggtttttcc gcgattacaa
    18661 gtttatatgt cattcctcgt cctcctgttc actcacaaaa tcagcttccg ggtcaaactc
    18721 ggtttcgggt tcctcgtcct ccaaatcctc gtcctcccca aaatcatact cgcccaaatc
    18781 gtcgctgccg gtggtttcag ccttgggctt tttgaacttg aaatagtaaa gggccgcacc
    18841 gccgcccgcc agcgccacca gcaggaacac gatcaggccg cccgcgccgc ccttgggttc
    18901 ttcctccggg gcttcgggtt cctccggctg gggttcctgc ggctccgggc cgctgcactc
    18961 ggtcaggttg gttttacata ccgggcaatt cgtgttcaca gccccggcct tgcatttcgt
    19021 ggtacaggag cagacagcag cgggggtgac ggtttccccg tcctccatca gcgtcagcag
    19081 atcggcttca tccacctgat ttaagaaatg gacagtttct tttccctcgt cgtcccggtc
    19141 aataatcaga taaaacacat tgccgttttt ggtttccacg gtgataaact gcttgccatt
    19201 tttcgcggcg ctgccgatgt cgtcaatcag tgacaggttc ccgtccgggg acagggcggc
    19261 gctggccccg ccgccattga acagttccag cagagaagca atttcctccg ggtcaatgga
    19321 gagggaaaaa ccgccctcgc cggtagccgc tacttccttt tcgctgacgg tcatatagtc
    19381 ggaatggaca tagccgatgc gctccggcaa gagaatttta atccagtcgc cgtctgtgcc
    19441 gactacctca accgtcgtcc cattgggaag ctgggtgaac gcggtcttgt caagccccgc
    19501 gccggtgcgg acattcagcc tgccgccgtt ggtggtgacg gtgccggtct gttcaggttc
    19561 ttttttgtcg gtgccggtgg tgatctggat gccgtcgtcc gtggcggcaa tgctcacctt
    19621 ggaaccgaac agcgcggaaa acaggtcggt gatgtccttg ccggtcagag ggccggtatc
    19681 tttttcggcc tcggtttctg tcccgtccgt ggcggtgccg gtttcgtctg tttcaggctc
    19741 caaaccgccc gtagcgggtt cggttgtagc ctccggcagc ggggccggtg ccgggtcatt
    19801 gccgcctgca agggcggtaa cgggaaaggc cgtgacgcag agcatcacgg ccagaagtgc
    19861 ggggagaata cgggaaatcc gtctggtctt attcttcatg ggcattgtcc tcctgttcat
    19921 ggtaaggggt tgcggcaggg gcggcctgca ttgcgccgct gcgtaagaac gcggcaaatt
    19981 cctccggctt catgttcatg ccgcgcacaa gctggatgat ctgcaaattc tcctgttcgg
    20041 ttttctgcgt ttccagctcc cgaagctgtt tctgaaactc cgcgatctta gacttggttt
    20101 tctggatgtc cttttcgatg cggtcaagtt tggttgccat aattgaaagc tcctttctgg
    20161 cgttaaggta aacgcccgta actgtaaaaa tgggattgcc agtagtttga agtaaggttc
    20221 gtgtaggaaa tcgggttccc gcaatgcagc atcacattgt tgcccacata caggccgacg
    20281 tgggacacgc cgggggtgtc gtaggttccc ttgaaaaata caaggtcgcc gggcttggcc
    20341 tgcgaactgg aaacaggggt gcagatgtta tataggccct gcgcccccag ccgtcccacg
    20401 ttccatccac tatgattgat tacccacgaa atgaagccgc tgcaatcaaa gctggtgctg
    20461 ggagagctgc cgccccacac atagggataa ccgatatatt tttccgcctc tttgagcatg
    20521 gcggcaaagg tggcatcctc caaagcctcc gggggtacgt cgtagtaggt cggcggcgtc
    20581 agggtggagg cgttggggta ctggctgccg gaaaacagat caggccggtt tcctagcgtg
    20641 gacatataca gggaatagaa acttagcgtt tcctcgtcca tgatatagac tggcacatgg
    20701 gacaggtcga agttttccag cgtgacgtag cagatgtaat agtcatacgg cacttggtag
    20761 gtgtctgtat gggtgttgcc ctccgcgtcc gtccatgtat cggtttcggt gcggtaacgg
    20821 gtttctacca cgacgttttc tgtcaggata tattgcttgt caaagagcat ggacaaatca
    20881 ccctgtacct ccccgatggt aaatatcccg tcatggagcg ccgacagaat ggaaatcagc
    20941 acataggggt catggctgat ctcgtcaagg tcaaaatggt attcgtcata gtcatgggtg
    21001 gcctcgtagt tgtccagata gtcttgcagc tcggcttcca gctccgcgta ggcgtcctcg
    21061 gcggccaaca tatcgctatc ctcggaaaag taagaggacg ccgtgatgcc gcttccggcg
    21121 ctgccgaaca tggcggtgca ggactgtaag ccgccgatga taagcagcat caaaagcccc
    21181 aacacaagga cggccagcgc ccctttccag tggcgggcca caaaggaggc cgcccgttca
    21241 ctttctttgg ctgcggcctt ggccgctttc gtggaggccc cggctgtctt ggccgcgccc
    21301 tgtgccgccg cgcctgcggt ctggcccgct gtccgggcgg ccttggcgta ctggcgcttg
    21361 atctgctgtt tctgccagaa ccgggaaatg gggttgctgg ccgcctgtgc gatctccggg
    21421 ttatcccgca aagatttctg gtagaaatac tccgcgttgg cagacaatag cctgcgctcg
    21481 gccttttccg cgtcccagta gggtttcagc ctgtggtgcc ggatggtgct gcggatggct
    21541 tttccggcct gccgctcggc aagttcctcc cccttatggc cgccctccac acccacgttt
    21601 tcatgctcga cttcatggat tttcccgtgg ccgaatagaa aaacctcgga aacaggacgg
    21661 cctgccggat tgggtttcag ggagggaggc cccttgtcct gtttgtcaaa gtgcaggacg
    21721 gtctttgctt tgccgcttgc ctcgtcaaag acggtttcct tgaccgggac gcgcttcttg
    21781 gggatggcgg cgcgggcctt atccagctta tcggcccgtt tgtccgattt tcggatatag
    21841 ggctgtaaat ccgggtcggc ccgttcttcc tccgacagtt ggagccgggc ggatggccgg
    21901 tgcagggcag cgtccccctc ggcggccaca gccccggcct gcttcgcggc tttcttggcc
    21961 ttatggccgt catggatggc gtcggcccgt tccagcagct tatcggcggt gccgcccggc
    22021 cccggcgcgg acaaatcctg ttcggcctcc cggctggaaa tgttctccac ctcgccggtg
    22081 gcctgatttt ccagcgtcag cccgtcgcgg gtcatggcct gcgtcacttt cgccttggct
    22141 ttcagctctt tcatggtggt tcacctcctt tgtggcgtcg ccgggtcata ctcccgccac
    22201 ctcgtttaag cgggtcgtca taatcttgta aagctgggtg ttgtgcggga tggggttggt
    22261 aaagggcagc accacgtttt caaagatcaa aaggccctcg ccgggttcgg aattgtcgat
    22321 gtacttctgc tgttcggtgg acaaattcaa gcgttccgca aggatttttc tatccccgga
    22381 cgcctgattg agcagcgtaa tgaagtcgct gttttcaagg atattttcaa tttccgggga
    22441 ggaaagcagg tctttgacgt tctgggtggc acctgttgga atgccgcccc atttcctaaa
    22501 ccgcttccaa atctccgcag aataggccgc cgtctgttcc tctttcaaaa gcaaatggaa
    22561 ctcgtcggca aaataccatg tggcctttcc ttggcttcgg ttggccgtga cgcgccccca
    22621 tatctggtct tggacaatga gcatccccag ctttttgagc tgcttgccca gctccttaat
    22681 gtcaaaggac acaagccggt tgccgatgtc cacgttggta cggtggttaa atacgttcag
    22741 ggagccggac acatacaggt caagggcctg cgccacccgg tcagcctccg ggacatgctg
    22801 atctaacaac gctttgtgga ggtcggacag gatcggcata ttctccgggc gggggtctgc
    22861 cagatagggc cggtagatca cgcgcacagc ccggtctatc acggtctttt caatggcttc
    22921 cagcccgttc ttgccgccca tgacaagctc acagaacgac agcacaaagt cggatttcag
    22981 ggccagcggg ttatcgtcct ccgaataatt caggttgatg tccagcgggt tcacaaagtc
    23041 cttgctggta ggcgacagcc ggattacctg tccgtggagc cgtttgacaa gggggtaata
    23101 ttcggcctcc gggtctgaaa taaaaatatc gtcaggcgtc gttaagaaca cactgacgat
    23161 ctcggattta cagctcatgg atttgccgct gccgggggtg ccgagcttca aggcattggg
    23221 gcaacgggcc tgcttcctgt ccagcataat catgttgcgg gacttggcgt tgatgccgta
    23281 atacatggcc gcgccgccct gaaaaagctc ctgcgtcaca aaggggacga acacggccac
    23341 gctggaagtc gtaaggctgc gctgtatctt gatctgattg acgcccagcg gcaggctgct
    23401 tgcaaggccc tgttcctgct ggtagtccag ccggacaagg ggacagttat gcttttgggc
    23461 cacgcctgcg gcttggaaca cgtcgttatt cagcttttgc ttggtgtcgg ccacgttgag
    23521 gacaaggaac gtcaactgga acagccgttc attgcgggtc tgcaactttg tcagcagctt
    23581 cttggcgtcc tcgccgtagg tggcaaggtc gctgggaaga atgtccatat cgtaaccgct
    23641 gcggacggcc tttttctgct ccgcaatctt catagcgtca aggtcggtaa ttttgcgctt
    23701 gatggtcttg atcgccttgg tctgttcaat ggcctgcaca tggagattga ctaaaacacc
    23761 gctttccgta ttgaggaagt cggccagcat atcgtccgac agctccgggg acaggatttg
    23821 caggaaactt accgcgccgt atttgccgcc catgccgaat gtctttgcat tgccgaagca
    23881 cagggaggac ggggcaataa aatccttggt ggagaggccg gacggggcca gccacttcca
    23941 gtcaaaattg aatatctcgc cgtccgggtg gtacacgcca tgcagcaggt tcagccgttc
    24001 ccagccgtcc atcacatggg ctacggcccc catgctcttg aaatagccta acaggtcaaa
    24061 gccgatgcgc ttcaatctgg ctcgcgccgc tttcacgctt tcggcctcga tggtgaatgt
    24121 caggtatttg gtctttacca tgccgttgtt gccgtttgca agctgctttt gcagaatgcc
    24181 ggtgtactcg gcccggatgt cgtcgaagtc gtccccctgc ggcgcgatct caaagctctt
    24241 ggcgtactgc accgggtcaa ctttccgatt gaggaaagaa aactggatat gaatggaggc
    24301 gtccacatag ttatacaggt cgcacagttt ttcaaagata gccgtctgcg tgtccggctg
    24361 ggcaagctga tagctgatgt ccgcaaactc aatgcacttg gaaaagacgc cgggcgatac
    24421 ccggcacacg ccgtccgggt acatgacctc gtaggggatg ctctgctgcg ccgtgtgggg
    24481 cctgccgtcg cccttatatt tacggattac cgcgtcaatt tcttttcgct gggcgcgggt
    24541 cagctttgtt ttttcggttt ctcttgggat ttcctttttg gacaatggcc tttacctcct
    24601 gttccatctg gtattgccgc acaagggcgg tgtaggtgtt gtcggtctga tagggacgct
    24661 cctttggctg gatgaagcag cactggatga tctgcataat caccacttca aggggctggc
    24721 cgtgacgctc gtacatggca agcaaaaagc ccggcagcat ggcaaatacc atgagcaggg
    24781 ccgccgcgct gttggtaaga tagtcacggg tgagaaaata taaggggacg ccgatcacca
    24841 gcgccccgga aaaacagatc agttgccgtt tggtaagccc aaacacaacc ttgcttttca
    24901 catgggttaa gtccttgggg acggttacat aagccatttg gcacctcctt tcacgtcagg
    24961 ataaggtcac attccacctc gttcccccac acgtcccagc cgggggtttt ctgtctggca
    25021 aacagctcca cgcggggcaa atcgcccgcc agctctacga ttttttcacg ggtcacatcc
    25081 ggctttttgc tgtgttgttc aatcgggctg ataataaact gatggacaga ggcagaacga
    25141 cgcttcggct tgccgtgtgt tgccagcagg cagatttccg cgtttcccct tgtccagcgg
    25201 cccagcccgt aaaaccatgt aaggctttta cggttctgtt tcagccatac aaaagccacg
    25261 gtcttgaatg aaaagcccca cgctttgatt aaccgcagag cttccggcag catggggaat
    25321 gtggcccaca aaaacagcag gcagtctttg gacgccagcc gttccaccgg cagggcgcac
    25381 agctcgtcaa tgctcatggt ggaatagtga ttttccgctg cgccctgtac gtttttgcag
    25441 tcataccgcc acggcgggtc agcgtaaatg atgttgtacg ttgaaataag caagttcctc
    25501 ctgtgtatgc ttggattttg ggatcaatgc gccccgaata cggcctttga tatactgccg
    25561 gtcttgaaca ggcagaagca cagcagcacc gtatagccca tacagcccca gattgcgccg
    25621 gatatgtcgg aagcggaacc gatattctgt atcagcaccg cgtagatacc caccacaacc
    25681 atgattaaaa aggcttggaa gcccagcgcc agcagggatt tcagataccc ttggcccatg
    25741 ccgcgccatt ccgcgttgcc cattgtggca agggggatag gccccagcga cgtgacaaca
    25801 tagatttcca ccatgcggcc ataggtcaca aggaaaatgc agatggaaag gatattcatg
    25861 gtgaggccca caacaagggt ttggaaccac agccccaaaa gagcgcccac gctcatagcc
    25921 tccaattggc tttcaaggtc tggtatcagg gtgttaaaat caatggatgt ctgcccgata
    25981 atcacaccgg cgctttgatt gacgacgctt tgggtggcgt cgaatacgcc catgacgatg
    26041 ttccatgtgt tcgttacgat caggattgca aacgcggatt tgaatatcca gcggaagaac
    26101 atggagctgt caaagtcgtg catattgttc ttttccgtta tcatctggat aagctcatag
    26161 cacatgacaa gggcgagtat ggccccggct atggggacga tcacggtttc cgacaggctg
    26221 tgcagcatat tgaaaatacc gctgttccat gcctgcgggg ttcgtcccac gtcggcggcg
    26281 atttctccga ctttggcatt taccgtatcg aacagcccgg acaggttgcc cgtgataccg
    26341 tccatgagga tgccttttat ccattcttca atgatttcac taatcacggg tagcacctcc
    26401 tttcccgcgc cctcccggtc tggccgggag gacgcggagg cgattagtta agggtttgga
    26461 cagggacaaa ggccaggatc agcccagcag cccggacagc aggggaacca gagtgacacc
    26521 gatcagggcc acgccgccgc cagcgacaag ctgcttgaca ccctgcgatt ttgcccccgg
    26581 attgtcctgc ccgtatccct ccaacagatt gatgccgccc catacgcaca gagcgccgcc
    26641 cagtccgatc acgatagtct gcaaagtagt aactgcggaa ttaaaaaatg ccataaattt
    26701 acctctttct gccggtttcc ggctcataat gattttttga ttttgttgtt ggaatgaaaa
    26761 aagccgctgt ttattcagcg gcggcgtcct cgtccgacag gtcgatttca tacaggtcga
    26821 atacttcatc ctgcgttacc acttgctgcc gtttccggcg cgtgatagtc aaaaatctgt
    26881 ccacgtcaaa ggtattcttc ttgtcggcgt cggcaaggta tttgtaccgg ggatgcttgg
    26941 taatatcgaa tttctccgat aaaaacgggc gcacaccccg tagctgcaag atacacttgc
    27001 cgccgtccat cacggcaatt tcgtcttggg acatcaactc ctttcctaat ttttgatagt
    27061 tcaatccgtg ggacgtttgg cttccccggt tctcgctggt gttgaaactg tcaatcgttt
    27121 ctttccccag cgcatcggaa atctccttgg catttttgcc gcgcccacta aggaaaagca
    27181 cactatcgca gttatccgaa atgatctcgg cggcgtcctt gtaaatcgct tttagctgcg
    27241 actggctctg caaaataata gaggccgata tttcacggct tcgaatggtg gcaatgagtt
    27301 tatcgaaatt gggtatttgg ccaatgttgg caaattcatc caaaatcagg cgtacatgga
    27361 caggcagccg cccgccgtac acatcatcag cccggtcaca aagcaggttg attagctggc
    27421 tttggagcat agccagaatg aaattaaagg tcgtgtccgt atcgctcata atgagataca
    27481 gcaccgtctt ttcgtccccg atggtgtcca gctccatttc gtcgtcctcc atcagctcgc
    27541 gtacctcccg gatgtcaaag ggcgcaagtc tggcaccgca tgaaatcagg atggaagaac
    27601 gggtcttgcc tgcggacagc aggaatttct tgtactggcg caccgcaaaa tggtcggggt
    27661 ctttctgctc caaacgctca aacatgagat cgacggggct ttgaaactcc gggtcgtcct
    27721 cgcgggcttc actggcgttt atcatttcca gcagcgttga gaagttcatt tcctccgctg
    27781 gggcctcgta ccagatatag ccgatcaggg ccgaataaaa caaccgttcc gacttcaccc
    27841 aaaaatcctc cgctgacttt tccccctcgc ccttggtgtt ggaaatcagg gtatttacca
    27901 gctttagcac gtccttttcc gaatggatgt agacaaaggg gttgtacttc atggacttgg
    27961 aaaagttaat ggtattcagg attttaatgc ggtagcccgc ctgctggaac atcctgccgg
    28021 tttccagaac caaagtgcct ttcggatctg tcaccacaaa cgaagtcgga tagtccgtgg
    28081 tcggaaagca ttgcatcagg ttgggtttca cgaaaaatct cgttttccct gaaccgctgc
    28141 caccgatcac cagcacgttt ttattccgcg ctgtcttggg gtctttgggc ctgccggtca
    28201 tggtaagccg ttccgtttgg gtgagcagga tattattttc aaacttgggg tcgatgtaag
    28261 gcgctatgtc tttggccgtc ccccagcggg cgctgccata ctccatgcct ttgcggtatt
    28321 tcttcacgtt cttgctcttg cagtacacca ccagccgcac gatcacggca ccggcgatac
    28381 cgatacacag gtcaaacagg tgcaggcttg gcagcgggtt ggcaaaagcc gccgcaaaac
    28441 catccgcaaa atgcaggagc ttccccgaca ggtcagcacc agcggccagc cggtacgcct
    28501 ccccgcattt cgcaaacagg tacacaaaaa tcagatacgg gaggttcagg atcagcaatt
    28561 tctttacgtc aggcttcaca gttccagccc cctttccttg attttctcct tggcccgctg
    28621 cttggtctgg gccgccgcct tttgcttcac agcggacaaa accttgcgga tagagggccg
    28681 ctgctccttg gaaagcgtct tggcggtaaa ctcccgaaac gccagatcaa ggttatctgc
    28741 atccttggcc ttgaaaatca caacgtagtg gggcggctgg gtggtcttat cttttcgtaa
    28801 ggtaaaatcc acgccgtatt tcttggcgca tggcttgaac gctccaatgt tggcctcggt
    28861 gatctcaatg ttggagagat cagccccatg ctttttaagc tgctttaggc tctgctggcc
    28921 gtggtgcagc gtgtttttgc ccttggcccg gtgttgcagg tactttttca gcgccgcttg
    28981 cagcacttga gccgtgagct tgcccgtttt catggacagg gcaatggtct tttgagtaac
    29041 ttcttcctgc aatcgcatcc ctccttgtca gaaagtcaaa agcggcgtct aaggcggaac
    29101 caccagacgc cgcaggtaga tataggtcaa atccgcaccc gcaggccgga aaggtcgtcg
    29161 gcccggatgc cgacaagata ccagttttcg gccatttcaa tgcgggtggg cctccatttg
    29221 ccgccgatca tcacgtcgaa acactccccg caatgcaggc cgccgtaata gtcggccaga
    29281 tcaaagcgga tgtcgtaacg gtctgttcgc tcgtcaaaaa tcaatgcgcc ctgcttcata
    29341 gggtcgtcct ccttttctcg ttacaggctt tcgccctcgc cgcaggaata gtccggcagg
    29401 ccggatttag gctttcccca atcgggctgt gccatgtcgt gcgcgacaag ggccgtgtag
    29461 tagttggaaa tggtgctggg ggcgttgaac agcatagccc gcaggtattg tttgatattg
    29521 cggactttcg ttgtgttctc ccgcagacag tcaaggacaa agcggatatg ttcgccggtc
    29581 agcttcatgt acttggattt caccagctcg gcagggtagt cgtccccggc gacacggatt
    29641 gtctttcggg cggtgcagac ggtttccagc atcaggtcta cgatttcgtc aagctgttca
    29701 cggtcaatgc tcctgtcctg aatgagatag gcatactcga tgttctccaa aatgatttcc
    29761 cgataaatcc gtacagcctc atcctttcct gtccttttcc gttccggcgg cgtagccgct
    29821 gcctcgccct ccataggggc aggggtcggg gatgggatag gaatggaatg ggtactttgt
    29881 ccatctgtaa ttattttttc tttttttgat aagtcagttc tttgtacttc tttatttaat
    29941 tgcgttggat tttccgacgc cggattatcc gatgtcggat tttccaacac cgggttatcc
    30001 gatgttggat tttccaatac cgggttttcc aataccggct gttcgtagat ggtatagacc
    30061 atatcggcca ttttgccttt tgcgtcacgg ccctgctcac gccggatata tccagctttt
    30121 tcaagctccc ataccgctgt cctgatcgcg tcgatgcttt cccggttgat ataggacagt
    30181 ccttttaggg tatagtccca atcctccggc aaggacaaca tctgcgacag caggcccttg
    30241 gcctttaagg tcaggttctt attccgcagg tggtgatttg acatcaccgt atagcctttg
    30301 ttcttttcca cacggaaaac tgccatagcg ttgttcgctc ctttcactgt gaatttgacc
    30361 gctgcatagt cggtcagctt gcccggctga tagggacact ctgcaaactg gcagaattga
    30421 tatttccaat ccgggcggta aaatcggcag gtgccgcaat cctccggcgc gtccgccccg
    30481 ccgccgtcgt agtgatcgaa accgggcttt tcctgcatca gccgttcaaa cgcccggtca
    30541 gggccggaag taaatagcat ggcgtcgcct cctttctcta tttgggcata aaaaagcggc
    30601 gtcctgttct cccatgacgg gaaagaggaa cgccgcgttt acggtgtcgt attcaattta
    30661 tgatgtgccg tatttggggt ggcgggtatt agcagtatgg ttcagcatat atgcggctca
    30721 atccgaaagt agtaaattgg tagtaaattg agttctcata tcctgcgaaa tgcccgtatt
    30781 tccggggctt tcagcgataa cctaaatttt tggaataaaa ctttcattat aggctactgc
    30841 aacatccatc caatcagcaa tctctttttg gatctcaggt cgtttccagt agccaacgac
    30901 gattttattt tgtttattta atcgagcatt gatgaaccca tattcgcggt caccatgagc
    30961 ggattgattt aaattcatga aatccatatc gatcgttttc caaggaatgc tttcgttata
    31021 ttgcgtagct aaatgaagca atggtttttg tagaagcttt gtcccgcgga tccacatttt
    31081 tgctggtgag aaagtatgca tccaagtgat cacacctgct acttcttctc gataattgac
    31141 ctctttcatg atttttgtga tcgtatcagc agtaactgct aattcttgaa ggcggattgg
    31201 ataaggcaat tggccatttt cattcagttc atcgaccatt tcttgtgcat gtttctttac
    31261 ttctcgcagt gcttcttccc catataaatg ctgtgatcct acaacaaacc aaaattcttt
    31321 ttctccaata ttcaacataa ttagccactc cttttatttg ttttgtccgt aatacgcatt
    31381 ttttccgtgt tttcgtaaat aatgtttatc taaaatccgt tgaggcagct cttctgaaaa
    31441 tgaattgagc tgtctggtaa ataaattcat cttgcatact tcatctagta cgactgcgtt
    31501 cattaccgca ctttgcgcat ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac
    31561 ccctggaatt gctaagggat caattttgcg ttctttgaat gtttctatga taacttttcc
    31621 tgtttcatat tcgtacccgc tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac
    31681 cgatccataa aaagtatctg catgcgtcgt ccccatcgca ggaacatcca atcctgcttg
    31741 tgcccagatc gtcgcccatg tcgagtgagt atggacgatt cctccgattt gaggaaatga
    31801 acgatataaa acagcatgag taggcgtatc cgaagaggga ttgagtttac cttcaatgat
    31861 atgattgtcc aagtccacaa cgaccatgtc gtctgctgtc atctcttcat aactgactcc
    31921 gctaggtttg ataacgaaat atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt
    31981 atatttgacg agtccgtgtt taggcaagtc aagatttgct tggtatactt cttctttcag
    32041 ttgttccagc attttctatc actccttttt cttgcaattg atgaatcgct gttttttcaa
    32101 tgtcgatccc ctcacggtaa cgtctcataa atatctgata gcctgctatt tcttctgctg
    32161 aaggtgtgat ggtgattcct tggtcttgtc caaacacgtc ttctgccaag aagttttcta
    32221 aagacttccc atgtcgatcc atcatatagg ctgctaataa cgcgattccc cacgctccac
    32281 cttctccggc tgtgtccatc actgtaactg gtgcctccat agcagatgct agaatccgct
    32341 gtccaacttc tggtgtttta aaaatccccc catgccccac taaacgatca atttggacat
    32401 tctcagattt caatatatcc attccgattc tcattgcacc aaaagcactg gctaaatgga
    32461 tccgcatgaa attcgctaaa tcaaaacggc tacctggtgt acgtacaaat aatggccttc
    32521 cttcatttac tcctgtaatg ttttcaccgg aaaaataacc ataagaaagt aatcccccgc
    32581 aatccgtatc gccttctagc gctttgttga ataataccgt gaataattta tctctgctta
    32641 tctccatacc caaagaattg gtaaattctt caaaaatact gacccaagca ttgatatccg
    32701 aagagcagtt attcgtatga accatgccta ctagttctcc tgaaggtgta gtgaccatgt
    32761 cgatttccgg atggacattt tctagttctt tttctaatac gatcatagcg aaggcggaag
    32821 taccagccga aatgttccct gtccgtttgg ctacactgtt cgtagcgacc ataccggttc
    32881 ctgcatcccc ttctggcgga caaaccggga ttcctggtct caaatttccc gtagggtcga
    32941 tcagataagc ccctgattcg cttaacttgc cagcttcttc tcctgctact agaactttag
    33001 gcaaaatgtg atccaagtca atcgctacgt gcgctttttc gattaactga ccaaaaatat
    33061 ttttcatttc ttgatggtag gttttcgttg aaggatcgat aggaaacatg cctgatgcat
    33121 ctcctacgcc tagtactttc tcaccagtaa gctgccaatg gacataacct gcaagcgttg
    33181 tcaaaaaatt aatttctttc acatgttctt ctttattcaa aatagattga tacaaatgag
    33241 cgatactcca tctctgcgga atattgtatt gaaatttttc cgttaatatt cgttctgctt
    33301 cttcagtgat tgtatttcgc caagtacgaa atggtactag taactgttcc ttgctatcaa
    33361 atgctagata accgtgcatc attgcactga acccaatcga cccgattgtc gtaagattct
    33421 cgccatagat ttcgaatact tctgctgcca tttttcggta actgacgcgc agtcctttcc
    33481 aaatatcttc taatgaatag gtccagatac cattttctag tttgttttcc cactcataac
    33541 taccagcggc aataggagta aaagatgaat caatcaatac agctttgatc ctagttgatc
    33601 caaactcaat ccctaaagat gtacgaccat ctatgacatc taacatactt tgcgcattta
    33661 cttccttcat aatgatttaa ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta
    33721 gtagcgctta caatgattat tgtatacttt tgtacgtaca tgtcaagcgg ttatttacaa
    33781 gaatacaata aaaaacactt gttttgtacg tacataagtt ataaaatgaa attgcttaca
    33841 aaatctgatc aaccgccttc tcgaaattct agaaggagag aatacttatg aaaaaagaaa
    33901 aaaaagtttc ctcatcgttt atttattttt ttggcgcatt tggcggcatt ctatttggat
    33961 atgatattgg tgttatgacc ggcgccttac cttttttaca gcatgattgg ggtttggcag
    34021 gtaaagcttc tttgatagga tggatcacgt cttcggtcat gttaggtgcg atccttggcg
    34081 gctctttatc tggtcttctt tctgataagc ttggacgacg aaaaatgatt cttctttctg
    34141 cacttatttt tatggctggt tctgtccttt ctgcatctgc ccctcacaat ggttcttatt
    34201 ttctgattgc agcaaggatt cttctaggtt tagctgttgg cgctgcatct gccctagttc
    34261 ctgcttatat gtctgaaatg gctcctgcta gactaagggg aagattgtca gggatcaatc
    34321 aagtgatgat tgcttctggc atgctccttt cctatgttgc agattatttg ctaaaagtgt
    34381 tgcctgaaac gatggcttgg cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt
    34441 tatttttcgg tgttttagct ctaccagagt caccgcgttt tcttatgcaa tctggccgct
    34501 tggaagaggc aaaaagagta ttgaactata ttcggacacc aaaagaagct gaacaggaat
    34561 tcgaacaaat ccagctgaat gtcaaacaag aaaagacaac cggaacttct tggcatactt
    34621 tgtttttaga aaaatatcgt tccttagtca ttgccggtat tggtgtagca gtattccagc
    34681 aatttcaagg agctaatgct attttttact atattccgct gatcgtagaa aaagcgactg
    34741 ggcatgcagc gagcgatgca ttgatgtggc cgatcatcca aggaatcatt ttagttgctg
    34801 gttctttgct ctttttggtt attgcagaca agttcaatcg gcgaacttta ttgaaaatag
    34861 gcggtagcgt gatgggactt tcattcatcc taccagctgt tttaggaact gtactggatg
    34921 ctcatacaaa ttcgttgttg attctcttgt ttttatgtat ttatgtcgca ttttattctt
    34981 gcacatgggc tccactgaca tgggtaatca taggcgaaat attcccacta gctgtccgcg
    35041 ggcgagcatc tggcctagcc tcttctttta attggattgg ttctttccta gttggcttgc
    35101 tttttcctgt gatgactgct tctatgtctc aagaaatcgt tttcggcatt tttgggatca
    35161 tctgcttttt aggggtttta tttattcaag agatcgtacc agaaactcgt gggaaatctt
    35221 tagaggaaat cgaacaaagt gcatctaaaa aaacttatcc taaacgtatt tctatccatc
    35281 attcttaaag aggttgtgac agaagtggtc agcttcaaga aataagaagg aaccgcttag
    35341 tttccagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgatga atattgtgac
    35401 ttgtgaccag ttggtaggag aacaccgttt agtggagagg tggtgaaaga aacgttcagc
    35461 cttgagcgat gacaaaagct ctcacaaaca ttgcgacgag taaccgtagg agcaataaga
    35521 ggcggtaaaa acccgtctct tatttttcgt tttctttcac tgtttcccgt tcaactagta
    35581 caggttcata gagaatattt ggctgtgcct gtcctttttc aatcgttgtg acgatccacc
    35641 gtgcggcatc ttgtcccaac ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc
    35701 ctaaatggct taaagaagaa tcgtctgttc ctacgatcga taggtcatct ggaatatgat
    35761 agccattttc tgtcaactta tctagcatta aactagctat ttgatcgtta tagcaaatga
    35821 tgccatctaa agcttgttct tctttcaacc gttgtactgt tttttcagcg attgtttctc
    35881 gtgtttccgt ggtgaaagta atgatatctt ccgtcaaaaa acgcacatta tactgttcac
    35941 acgcgctgat aaaacctttc atcctgtatt ttccttgaag atcgtcgatt tttgtgatga
    36001 acaacagacg ctgatgtcct ttttttatca aatattctgc tgccataaaa ccgactttca
    36061 catcatctaa acaaatataa gaggcattta actcttcata agcagcattg atcatcacca
    36121 tcggaatgcc ttgttctctc aaagcaacat aaagtgctaa gtttgggtta tactgattac
    36181 ttttcgtagg ctcaacgatc aaaccatcta ccccataagc aatcattttc ttcaaacact
    36241 ctttctccaa ttcgtgatcg ttattcgtac tggctaaaag caatgaatac ccagattctc
    36301 gtaattcatt ttcgattcct cggataatcg acggaaaaat ataatcagag agatatgtgg
    36361 tgatgacacc gatcgttttt gttctgttca atggtgtttt ttctttctta tagctgtcat
    36421 ctacatatgt gcccgaccct ttttctttac gcagatagcc ctcatttacc aacacagcga
    36481 ttgcttgccg aatcgtatgt ctgctgacgt catatatttc ttgcagcttg aattctgagg
    36541 gaatggttgt atttacttcg tattcctcac gaaggatctt ttgttttatc tcttctgcaa
    36601 tatcttgata ttttgccatt tgccttgccc ctttttattt gtacgtacat atatcataac
    36661 gagtctgcaa ggtttattca agcaaaaatc aaggtttctt gtgctcataa ggcatacgga
    36721 acaggttcac caaattctgg aaatccttgc tcattccacg taaagcattg cgcgttcgcg
    36781 tgccgattag gattgtctaa cggatcccct ttttggttct tctctggtcg agcatgatag
    36841 atcaaaatat caaagcaccc gtcttctgaa accgtaaacg agttgtgacc aggaccaaat
    36901 tgcttatttt tttcagaaga aacgaatact ggctccgata atttatgcca tgattctttt
    36961 aataaaagat tcttgtgctc atcagcccac agcaggccca tggcatagtt ttcatctgtt
    37021 gcactaccag aatacgtaat gaatattttc ccattgcgag taaggactgc tggaccttca
    37081 tttactgaaa aaccccgttt ttcccattca tactcaggta tagaaaggag gatctgcttc
    37141 ccctttaacg tccaaggatt atccatttcg gatagataaa gattagaatt cccagtaatt
    37201 tcaggatctt gttgggccca cacataatat agctttcctt gagaagagaa agttgttccg
    37261 tctaaactga aagtttccct tttcgttttt atttgtccct tttctatcca atctgtattc
    37321 attggatcta tatgctgatt ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt
    37381 atagttttat gattgcttgc agcaaagtaa atataccatt ttccattgat ataatggact
    37441 tcaggtgccc agatcaattc actcatcggt ccttcttcat gagcctgcca tacaatcagg
    37501 cttgcagcat gttctaattc attcagtgac tttgcccttc ttaattcaat cacttggtag
    37561 cctggaaccg atcctgtaaa ataataataa ccatctgtat gtttatatac ccaaggatct
    37621 gcccgttgcc gaacgatagg attattataa agtgtcttca tcataacaaa tttcctcaca
    37681 ttttctttat tctaattaca ttccaagagt atttttctat ccctacattt aattcgtcat
    37741 gtttttctat tgaaaaatag tttatttcag gttttatttt ttctggttct tcacgtgtat
    37801 ttttgtccaa cagatcgcta ctggttaatg tgtagtgctc tccttgatct agcaaattga
    37861 aatcatgttc aatcgttatc ttttgttgtt catttgtgcg attgatcaag aaaatagtca
    37921 gctgattgcc gttatctacg atcacactgt ccacataagg aacatttttt atcttttcac
    37981 aatcataaac agggcaaatg atttgttgtt tcaatacttg acctttagca tatttcgata
    38041 agtgttgcat cacataataa gttggattcc tccatgcttg tcctttagca tctgtcagta
    38101 ttaaaggtat tgtgtttact aacaatgatt gacaactaat ctttacgcga tctgcgtgtc
    38161 gtaaaatagc cagccctaat gaaccaaata ataatgtatc ttccatagta aagtaacacc
    38221 aatcgatggg actaccgact tcaaaatccc tatgtctttt ttccggttgg gcatgtacat
    38281 tccattcatc aaacgctaga tacattgttt tctcacttct caaatatcct tttacataat
    38341 cacaagtagc aacgatttct tcaatttgac gatcgaatcg ataagctttt gcaaggtatg
    38401 tcgctgtatc atcacgctca tttttaggag gtttagtcaa gtcttcttca tcatacttat
    38461 caatatagtt atgcaaagct aaataatcta cattctcata agcttcttct aatgcgatac
    38521 gatcccattc agggtacgat ggtaaacgtg gcgtagagct tccaactaag attgtttcaa
    38581 ttgaatcatc cactaacttc atgacttttg aggcttcatt tgctaaccta ccatattcat
    38641 aagcactctt agcccccact tgccatggac cgtctagctc attccctaaa caccataatt
    38701 taacgccaaa aggctttttt tgcccatttt ttattcttaa atcactccag aaggttcctt
    38761 ttggaaaatt acaatattct actaaatttt ttgcttcatc aattcctttt gttcctaaat
    38821 tcaccgtgaa tatcggtttc gcttgtacat gactgatcca tttaaaaaat tcgtggagtc
    38881 caaaagaatt tggttctata cctcgccatg ctaaatcaat tttttttggt cgttgttctt
    38941 tcctacctac agtatccacc caatcataac cagatgtaaa gtttcctcca ggatagcgaa
    39001 tgacacctaa ttctaattcc tttacataag agaggacatc ttgtctaaac ccgtcaccat
    39061 cagctgtttc atggcccggt tcataaatcc ctgtatagac gacacttccc atatgctcaa
    39121 caaatgagcc aaacagccga gaactgatct ctcctaccgt aaaatcctct ctgattttta
    39181 ctttcacttc ttcgtccaac agctttccct acctttcatt tcctatagtc aagataatag
    39241 atacaagttt ccatacgcca ccagcaaaga taaaaataaa aggagggaaa agaaatatga
    39301 gcagtacaaa tcctaataac aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata
    39361 ctttccatgc actttgttca aaaatttgta aaaatgtgat atctttgttt tcagaaatca
    39421 catatatcca ggataaaaga aacatggaat aaataagtaa aactacatat ccactaattg
    39481 aaagtacgat tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta
    39541 acagactaag gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa
    39601 tgatatttcc tgatccctca cctttccatt gcgccattac ataaaaaaca gcacatgtag
    39661 caggcacgat agtaataaca ggcaaacaca cgattaccca gagaatacct aataagatgt
    39721 aatcagaaac cacaatcaac tgtccataaa atttagtctc aactattttc atcttcctca
    39781 ctctttcatt ccagacatag ctgttccttg aacaaaatat ttctgtgcaa aaatataaac
    39841 cactagtaat ggcagcattg aaataaccgt tccagccatt acaggtccat aaaaagaaat
    39901 gttctgccct gtaaataaag ctaaccctgc tggtaaagtt ctcatttttg aactacttgt
    39961 caatatcaat ggataaagca agtcattcca actattcatc agcgtgaaaa tccctaatga
    40021 taaaagagta ggtttgctca atggcaacat gattttcaaa aaaatattaa actcacttaa
    40081 tccatcaatg cgtgctgcct cttccaaaga atctggtaaa gtaataaaaa aggaacgcat
    40141 catgaagata ccaaatgcag ttgtcatacg aggaatgatc aaacctgcat aagtatcaag
    40201 catgccaagc aaattgactt caataaatag aggaatcatg aagacttgga atgggatcat
    40261 cattgtcagc aaaacgaaat agaacaaaac tgattttcct ttgaaacgca tacgagcaaa
    40321 tgcatacccc gcaagagaat caaagaatac agaagtgatc actgtccctc cagcaaaaat
    40381 cactgtattt ttgatgtaat ctaataaagg gatcgttgtc caaaccttaa tatagttatc
    40441 tagggtatag ctatctgcaa atattctagg cggaaatgag ataatgtctt tttcgtgttt
    40501 aaaagaagaa ataatcagcc acaaaaaagg aaacgcaata accaacaata taactagcat
    40561 actaatcgtt cgaatgattt ttttaccact aactgctttt cgactattca cagtaactgt
    40621 aatcgagctt tctggtgcca tacaggtttc cctcctattt catttgtttt tctttccgtg
    40681 acatgtacac attagtggag atagctatag ttgcaacaat gataaacaaa accgaagaca
    40741 gtgctgatgc atagcccaat tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa
    40801 cagctgtttc tgtcttaaat tgtgggccac cagcggttgc aacatatact tggtcaaaca
    40861 cttgcatcga accaatcaga ttcgttaaaa tacaaaaacc taaggaggga acgatctgag
    40921 gtagcgtaat gttaaaaaac tgctgtcgat ttgttgcacc atccatttga gcagattcat
    40981 acaaagaggg agagattccc tgaatagcag tcaagagaat cgtcatagta acaccaaaat
    41041 tcttccaaac agtcatgatg gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt
    41101 gagggatcgg caagccgact tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa
    41161 gcaacataga aaaaataatc gcaattgacg tcaatgaaca aataactgga atgtaaaatg
    41221 tcgaacgaca aaaacgattg aatctggtat ttttagataa tgctactgct gctaataaac
    41281 caacgataat ttgtgttggc gtttcgagta cggtgaaata taaagaatga atcaacgaat
    41341 taactgctcg ttcatcacta aatatacgtg tgaaattttc aaaacttaca aaatttcttg
    41401 atgtaaagaa aatattcaaa tctgtaaaac taatagcaaa tgttcctaaa agcggtatga
    41461 cagtaaataa taataaaact aaaaaagatg gagttaaaaa caaatacgca gcacgatcag
    41521 gacgattcca atatttggtg aatccgttca tatagtccac ctcatctttc attttgataa
    41581 aatatacaat cttggtttaa acgaatctga ataagatatc cgaacaatcg ctgtgatttt
    41641 tgcaataaca cctatctgca aatactaggt cgatcattca gatatctgta tcttgccaac
    41701 agtctatcct gtataaagtt ctgtactatt tttgcagtaa ttgattgatg gcatcatctg
    41761 catcgttcat gagtgtctca ggatcttctc ctgctaataa tttttcaatc agtggattga
    41821 tgatatcgtt attgatcgca gagatttggg taaaacctgg taaatatggc tctgcatagt
    41881 ccatttgttt cgataattca gatacaatag gatcattttt tacttcttca tcttttgcta
    41941 ctgaatccaa gtaggcagga aaaccatttt cgatactcca ttttttacca atttcagttg
    42001 tattccaata tttcaaaaac tcatagacag cttctttttt attattatct gtacttgccg
    42061 gaatggcata tcctacgcca tctaaaatag ctgttttttt gtctgaatca ctcccttgtg
    42121 gtaaggtagt cacattataa tcaatctcgt ttgctttcaa tccgttattt aaccatggcc
    42181 cattgatttc aatggctagt tgaccagcat tcattaaatt gtccatttcg gcgccagaaa
    42241 tcgattctgg accagtcttg tctacatgga tcattttttg gatcgctttt aacgttttaa
    42301 ggttctcagg agacgcaaat tcagacttag tataatcatc gttcactaat ttaccgccat
    42361 ttgctaacat ccagttatat aacggtgcag ttccatcctt attgaagaca aaacctgata
    42421 catttttact actatcagtt aattttggtg ccatttctgt caattcttcc caagtagtag
    42481 gtggttgttc agtatctaat ccagcttgtt taaacaaatc attattccaa tacatataca
    42541 tgccttgtac ctgcatgggg acaaaatact gtttaccttc tacttcacct aattcaatag
    42601 ccgtttcttt aaaatcagat ttatctactc catcatattc ccagaaatcg tctaatggtt
    42661 gtaccgctcc gttttctaca tactgtgcaa aatctggata atttaatgca ataaagtcag
    42721 gtgctttttt agaagaaatc gctgtaggaa gtttttcatt caaattggcc caagtcatga
    42781 tatccatttc aattttgatg tttttatcat ttgttttatt aaattcatta acgatttctt
    42841 gcaatatttc accatcagat gcagtaaacc cattccaaaa agtcaaagtc acttctttat
    42901 cagaatcaga ttctgctgaa cttccgccac cgcaagctcc taaaatacct gttgcagcta
    42961 ccaacaaaga gccaattaac cattttttac ctttcatttt ttgttcctcc ttcatctgat
    43021 aaacgacatc tgaccacatt ccaagatagc ggtttcaata aacaattgat tttcccatct
    43081 tcagctttta catttgttga atgacttggt tttacttgtt cacttttagg aagattagtt
    43141 gcttttgtat caaatccact catttcagaa aaatcaatca ctccctctaa tgataattca
    43201 gacaagtgaa cagttagatt catttcttct tctccacggt taactgcaaa tatgacaagt
    43261 tctttgtcat ttattacggc tatactttct aaatacggca cttcttcaaa atcttccgtc
    43321 gcatagtttg ctgaacgaat ttgtgggctt aacacctctc cttgtccata cacagcagct
    43381 tgcataaatg gataatagat cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt
    43441 ggtgcaataa cgtttaccaa ttgagcaaga caagcaatct ttactctatc tgcatgtttc
    43501 aataaagtga ttaatagaca gcctaatagt aatgcatctt caaaattata gttatcttct
    43561 agtaaaggag gagcaatctg ccaaggcgct gcttgctcat cttgttccgc tgaatgatac
    43621 caaatattcc attcatcaaa ggacaggttg atctgcttct tactatgttt cttcgccttg
    43681 actgtatcgc acatcgctgt cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca
    43741 agataatgag aaagatcatt tttttggttg ccataatatt gatgcagaga caaataatct
    43801 atttgatcgt acgcttgatc aagaactgtt aactcccaat caccaaatgt aggcatttgc
    43861 cgattcgaac ttccgcataa aaccacctca attgaatcat ctacgagttt catcgcctta
    43921 gccgtttcct ctgctaaccg tccatattct tccgctgttt tatgtccaat ctgccaagga
    43981 ccgtccattt cattccctaa acaccatgtc ttaattccaa aagggctctc ttgaccattc
    44041 gaacgcctta aatcactcca ataagttcct ttaggaaaat tacaatattc gactaaatta
    44101 cgagctgcat ctactccacg agtacccaga ttgacagcca tattgatttc ggcccccaca
    44161 tgctgacacc agttataaaa ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa
    44221 gctaaatcca accttctagg tctatttgct atgggcccaa tactgtcttc ccaacgaaat
    44281 cctgacacaa aatttccacc aggatatcta atcatgggaa tgttcaattc ttttaccaag
    44341 tgaatgacgt cttggcgaaa tccactttta ttagctgatg gatgatctgg ctgataaatc
    44401 ccttcatata ctgctcgtcc catatgttca ataaatgaac tgtacaaccg cggatcaact
    44461 ttgcctacac gcttgacact actaatcaac attgtggctt ccataactga cctcctttaa
    44521 tttatacatg aataatacac ctcgttgaaa gcgttgtcaa taatataaat gttaaactta
    44581 cttatttttt atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta
    44641 atataacttt tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata
    44701 tatgcaacta caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac
    44761 aaggatcaag atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa
    44821 agaacttgga gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa
    44881 catcatcaaa acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat
    44941 cgatattagt tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc
    45001 tattggtcac tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga
    45061 ttttatcggg aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg
    45121 cattctctgg tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga
    45181 ggatacattg gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa
    45241 tgacaattgg ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac
    45301 gagccctgga gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa
    45361 tcttaatcaa tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa
    45421 tggagaaccc ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca
    45481 tttgcgaatc gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa
    45541 aggattcggt aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc
    45601 ttttctattt tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt
    45661 attgttattt ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct
    45721 tgttccacca tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat
    45781 aatcatcaat ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc
    45841 catcttcaag atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt
    45901 gaagcaattg tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac
    45961 catcaccaaa tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata
    46021 cggctcctgc tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga
    46081 tatttccttt gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg
    46141 catcaacagc tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag
    46201 cagcttttgc atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt
    46261 gatagttttc atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc
    46321 ctgctaaagt agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat
    46381 attttttcaa atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa
    46441 ttgttggttt gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc
    46501 ctatgcttct gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt
    46561 atggttccag cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt
    46621 atactcttcc catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc
    46681 aggtaattcg taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt
    46741 aaagtctcga acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc
    46801 ttcttctcga gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa
    46861 atttcgagtg atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct
    46921 tgataacttg atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa
    46981 tactgcacca agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac
    47041 atcaatttta gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga
    47101 agcatagatt tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga
    47161 tatataggcc atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa
    47221 ttctttcgcc acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac
    47281 taagaacacg cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc
    47341 ataagattgg actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc
    47401 agcacgtaca ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac
    47461 tgctgcatcc agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat
    47521 gacagatttg atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa
    47581 cagtgctgtt tctactaatt ggcagtgcgc taaaggccct tccacttgga tcaatggttc
    47641 atttgcaaaa actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt
    47701 ttgtaaatac tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc
    47761 ggaatcattg aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac
    47821 cgcgtatccg tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc
    47881 agctcgacct aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag
    47941 cgtcaaactg tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt
    48001 catttcctac ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg
    48061 aaaaagcaca tgttttacgg atatttcttg ctaa
//
LOCUS       DAZULG010000011        45996 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000011 DAZULG010000000
VERSION     DAZULG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.011"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(253..621)
                     /locus_tag="ACG9CY_000523"
     CDS             complement(253..621)
                     /locus_tag="ACG9CY_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS4937062.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            771..1253
                     /locus_tag="ACG9CY_000524"
     CDS             771..1253
                     /locus_tag="ACG9CY_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS4937063.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1290..2300
                     /locus_tag="ACG9CY_000525"
     CDS             1290..2300
                     /locus_tag="ACG9CY_000525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS4937064.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2415..3128)
                     /locus_tag="ACG9CY_000526"
     CDS             complement(2415..3128)
                     /locus_tag="ACG9CY_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937065.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3301..3908)
                     /locus_tag="ACG9CY_000527"
                     /pseudo
     CDS             complement(3301..3908)
                     /locus_tag="ACG9CY_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            4392..4619
                     /locus_tag="ACG9CY_000528"
     CDS             4392..4619
                     /locus_tag="ACG9CY_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS4937066.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4635..5801
                     /gene="hemW"
                     /locus_tag="ACG9CY_000529"
     CDS             4635..5801
                     /gene="hemW"
                     /locus_tag="ACG9CY_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS4937067.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6035..7078
                     /gene="hrcA"
                     /locus_tag="ACG9CY_000530"
     CDS             6035..7078
                     /gene="hrcA"
                     /locus_tag="ACG9CY_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS4937068.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7172..7735
                     /gene="grpE"
                     /locus_tag="ACG9CY_000531"
     CDS             7172..7735
                     /gene="grpE"
                     /locus_tag="ACG9CY_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS4937069.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7786..9615
                     /gene="dnaK"
                     /locus_tag="ACG9CY_000532"
     CDS             7786..9615
                     /gene="dnaK"
                     /locus_tag="ACG9CY_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS4937070.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9766..10932
                     /gene="dnaJ"
                     /locus_tag="ACG9CY_000533"
     CDS             9766..10932
                     /gene="dnaJ"
                     /locus_tag="ACG9CY_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS4937071.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11014..11643)
                     /locus_tag="ACG9CY_000534"
     CDS             complement(11014..11643)
                     /locus_tag="ACG9CY_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4937072.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            11655..11828
                     /locus_tag="ACG9CY_000535"
     CDS             11655..11828
                     /locus_tag="ACG9CY_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937073.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11873..12610)
                     /locus_tag="ACG9CY_000536"
     CDS             complement(11873..12610)
                     /locus_tag="ACG9CY_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4937074.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12612..14114)
                     /locus_tag="ACG9CY_000537"
     CDS             complement(12612..14114)
                     /locus_tag="ACG9CY_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS4937075.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14373..16367
                     /gene="uvrB"
                     /locus_tag="ACG9CY_000538"
     CDS             14373..16367
                     /gene="uvrB"
                     /locus_tag="ACG9CY_000538"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS4937076.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            16377..19193
                     /gene="uvrA"
                     /locus_tag="ACG9CY_000539"
     CDS             16377..19193
                     /gene="uvrA"
                     /locus_tag="ACG9CY_000539"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS4937077.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19456..20721
                     /locus_tag="ACG9CY_000540"
     CDS             19456..20721
                     /locus_tag="ACG9CY_000540"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS4937078.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20725..21456
                     /locus_tag="ACG9CY_000541"
     CDS             20725..21456
                     /locus_tag="ACG9CY_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS4937079.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            21591..22475
                     /gene="rapZ"
                     /locus_tag="ACG9CY_000542"
     CDS             21591..22475
                     /gene="rapZ"
                     /locus_tag="ACG9CY_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS4937080.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22472..23470
                     /gene="yvcK"
                     /locus_tag="ACG9CY_000543"
     CDS             22472..23470
                     /gene="yvcK"
                     /locus_tag="ACG9CY_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS4937081.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23504..24439
                     /gene="whiA"
                     /locus_tag="ACG9CY_000544"
     CDS             23504..24439
                     /gene="whiA"
                     /locus_tag="ACG9CY_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS4937082.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            25007..25663
                     /locus_tag="ACG9CY_000545"
     CDS             25007..25663
                     /locus_tag="ACG9CY_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937083.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            25842..26407
                     /locus_tag="ACG9CY_000546"
                     /pseudo
     CDS             25842..26407
                     /locus_tag="ACG9CY_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27106..27627)
                     /locus_tag="ACG9CY_000547"
     CDS             complement(27106..27627)
                     /locus_tag="ACG9CY_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS4937084.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            27825..31505
                     /gene="nifJ"
                     /locus_tag="ACG9CY_000548"
     CDS             27825..31505
                     /gene="nifJ"
                     /locus_tag="ACG9CY_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS4937085.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            31694..32581
                     /gene="cdaA"
                     /locus_tag="ACG9CY_000549"
     CDS             31694..32581
                     /gene="cdaA"
                     /locus_tag="ACG9CY_000549"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS4937086.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            32578..33747
                     /locus_tag="ACG9CY_000550"
     CDS             32578..33747
                     /locus_tag="ACG9CY_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS4937087.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            33771..35126
                     /gene="glmM"
                     /locus_tag="ACG9CY_000551"
     CDS             33771..35126
                     /gene="glmM"
                     /locus_tag="ACG9CY_000551"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS4937088.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(35198..37003)
                     /gene="glmS"
                     /locus_tag="ACG9CY_000552"
     CDS             complement(35198..37003)
                     /gene="glmS"
                     /locus_tag="ACG9CY_000552"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS4937089.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            37413..37703
                     /locus_tag="ACG9CY_000553"
     CDS             37413..37703
                     /locus_tag="ACG9CY_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937090.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            37716..38741
                     /gene="sppA"
                     /locus_tag="ACG9CY_000554"
     CDS             37716..38741
                     /gene="sppA"
                     /locus_tag="ACG9CY_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4937091.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            38744..39403
                     /locus_tag="ACG9CY_000555"
     CDS             38744..39403
                     /locus_tag="ACG9CY_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4937092.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            39560..40492
                     /locus_tag="ACG9CY_000556"
     CDS             39560..40492
                     /locus_tag="ACG9CY_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937093.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            40646..41935
                     /gene="tig"
                     /locus_tag="ACG9CY_000557"
     CDS             40646..41935
                     /gene="tig"
                     /locus_tag="ACG9CY_000557"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS4937094.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            42182..43432
                     /gene="clpX"
                     /locus_tag="ACG9CY_000558"
     CDS             42182..43432
                     /gene="clpX"
                     /locus_tag="ACG9CY_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS4937095.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            43515..44102
                     /gene="yihA"
                     /locus_tag="ACG9CY_000559"
     CDS             43515..44102
                     /gene="yihA"
                     /locus_tag="ACG9CY_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS4937096.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(44176..44331)
                     /locus_tag="ACG9CY_000560"
     CDS             complement(44176..44331)
                     /locus_tag="ACG9CY_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS4937097.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            44467..45795
                     /locus_tag="ACG9CY_000561"
     CDS             44467..45795
                     /locus_tag="ACG9CY_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937098.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            45806..>45996
                     /locus_tag="ACG9CY_000562"
     CDS             45806..>45996
                     /locus_tag="ACG9CY_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937099.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTR"
BASE COUNT        15167 a         7903 c         9861 g        13065 t
ORIGIN      
        1 attttctcgt attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt
       61 ttactaagta gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact
      121 ccttataaaa atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga
      181 taggattgaa caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat
      241 tcgtataaag ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga
      301 aatcaattgt tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt
      361 atccgtatat acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt
      421 ttccggtgcg atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg
      481 gtcagtaaac ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt
      541 tatcttagca aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat
      601 gatcggctga aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc
      661 agaaaaacaa tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat
      721 atttatatga taagatgtct caaataataa aatagaaaag agacataaaa atgattgact
      781 tggcaaagaa attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt
      841 atacaaatga agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca
      901 aaagcttatt cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga
      961 aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat
     1021 cagaacgcat ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg
     1081 aaaaacagat tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg
     1141 caccaggtag accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc
     1201 agctacttga gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta
     1261 aaatagtgca tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg
     1321 caacgattgg ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg
     1381 aatcagcttt aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt
     1441 ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa
     1501 tgatgcaaga tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg
     1561 agattctcga ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag
     1621 atattagtac gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa
     1681 ctaaccttat tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg
     1741 tactgaaaac aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag
     1801 tgtggcccca tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc
     1861 aatcagtcag cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata
     1921 cgatcaggca tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata
     1981 atgatggaaa gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa
     2041 gaagatcact tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt
     2101 tcggtagaag tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc
     2161 tgtcaaagga attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac
     2221 aactgacatt gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg
     2281 ttttgcaaac gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa
     2341 atctaaatag aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc tttttgtata
     2401 acatattaga aagtctaatt atctataact gtcacatcga ttaactgttt cacgtcattg
     2461 ggatttcctg taggaatagc tactacagat gcattatcaa tgatttcttg agttgtttct
     2521 ctaatgaacg ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga
     2581 taactagtga ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat
     2641 ttcccagtga gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg
     2701 ataggtgttc ctgcaggata atgaataaca tgtaattcga aatcacccga tgtatctcta
     2761 aaagtattac ctctaatagc tagccaaaca cgtgcgtact caatttgtag atcagaatat
     2821 cctgctaacg gatctattga ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa
     2881 gaaatatttg tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta
     2941 ctgcttgttg aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca
     3001 gctgaactgg tttgtctgat tgaacttttg tctgtggtcg cagatggatc actattttct
     3061 ttactaggag acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac
     3121 tttttcatat aaaatacctc attatttttt taataccagt atagcataat cctgaatttt
     3181 tgaatccgta aaaccttgtc tatgaatata aatagagtgt atcttctttg ataaatgtca
     3241 gaaaaagtgt gacaaataat catcagtaaa attacttgtc acactattga taaggagtat
     3301 ttaattgaag agaatgatta ggagaaagga tctccgtact ttttatagta atcatagaat
     3361 ccaagtttgt atagacgaat cgttttttct cttatttctt ctgtttcaaa atggactgct
     3421 tgaagaatca agtctgtgaa ttctatagga gctgttccat tcgcagtaat caaattacga
     3481 tctttcacag cttgttgttc tataaaatct tctttattct gataattttc cgattctaac
     3541 caaggaaatt gcgcatttcc ggtgtgtttg taaccttgca ataatccgct ctgagctaaa
     3601 taatcaactg ccccacaaat agcagctact ggtacgtctg tttttaacaa tttatcgatt
     3661 atttgatata aatgatgatt ttttatgtcc catgaattgc caccgatcat aataaaaagg
     3721 tcaacttttt caggtatttg ttcgattaag taatcgattt ttgtttttaa tcctccaatg
     3781 gaagtgacct ctgattggat agaagcagtt ttgttttgcc atttctcatt ttggtttagt
     3841 gtgctagtaa tataagcacc ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca
     3901 gttttcattt tcaagctctc ctttactatc tataacaagg acaacagttt taacctacag
     3961 acattcatta aaattgggta gcgacagcga aaacaacaat tcttaagttt tagacagagt
     4021 atacagatag tcaacttaat taaatacgaa ctaaataaaa atgacaggta atcatttttg
     4081 tttaccagca ttataacatt ttgcatacat ggatgacaaa agaaccaatt cccacttgct
     4141 tgagtacaat attaaaagaa actggaaggc aaaaagtaat tatagaaacg aaaaataaat
     4201 catatcgggc actcagacat gaaaacaacc ctagagatat ataactaggt aaccagttta
     4261 atgaaagaaa aaatcatcca agaagtcgat tcttggattt tttagtggat ttgttcgcca
     4321 aatacgttta gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc tgagcactta
     4381 aggaggaatc tgtgaaaata gcttttcata tttttgcaga ttttgactta atgccgaagg
     4441 tcagctttgc gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca
     4501 ataagcaagc atttttagag ctaggtttcc ttgattctaa atatgcggct tatttcaatg
     4561 agttggtgct tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag
     4621 gagaaagaat ttcaatgata gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca
     4681 ttcatatccc attttgcgag catatctgct attactgtga ttttaataaa gtttttttag
     4741 aagggcagcc agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag
     4801 aaagtcatcc gacagcgaat ttagaaacac tttacgtagg gggcgggaca ccaacttctt
     4861 tatctgctaa acaacttgat cgactgttat caggtgctag agagatcctg ccttttcaaa
     4921 atggtaatga gtttactgta gaagcgaatc caggtgattt gacccgtgaa aaacttcaag
     4981 taatgaaaaa ctatggcgtc aatcgtttat ccatgggagt gcagacattt gataatcgct
     5041 tattgaaaaa aattggacgt aaacacaccg cagaagatgt gtatcaaact atgcaatttt
     5101 tagaagcaga aaattttacc aatgtaagca tcgatttgat ttatgcatta ccgggacaaa
     5161 cactagaagg atatctggat actttggaaa aagctttggc tttggatctg cctcactatt
     5221 ctttgtactc attgatcctc gaaaacaaaa caatgttcat gaactgggta cgacaaggaa
     5281 gactcgaact gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa
     5341 tggaaaagca tggacgtcac caatatgaaa taagtaactt tgggctctcc ggacatgaat
     5401 ctcaacataa cctcatgtac tggaacaatg cccactattt tgggtttgga gctggtgcta
     5461 gcggttatct tggagatatt cgttaccgca acaaaggacc aatccaacac tatttacgcc
     5521 cattacgagc aaatgaacgc ccggtattgg aagaagaaaa attgacacga aaaaatcaaa
     5581 tagaagaaga aatgttctta ggactaagaa aaaaaacagg gattactaaa aaacatttct
     5641 atgatcgcta tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg gaattagagc
     5701 aagaagggtt acttgttaat gaagaagacc gtgtttattt gacaccaaga gggacctttt
     5761 tggggaacaa tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt
     5821 ttatctgaat ttaaataaaa aaagttcaaa aatacaataa aggactattg aaacgttgat
     5881 ataactggat ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa
     5941 tggagtcttt ctcttgacag gagaagggct tcttgctata tttataagtg gattagcact
     6001 tagaatatat gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat
     6061 tttacgtctg atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt
     6121 gatgtctgag ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga
     6181 agatgaagga ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg
     6241 ctatcgttat tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct
     6301 gctgattcgt cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc
     6361 tgctgagata ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa
     6421 agaccgccga ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat
     6481 cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg
     6541 aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct
     6601 gatgacggtg taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca
     6661 aacgacagaa gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagataa
     6721 ggtttatgtc agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca
     6781 gttcaaatcg atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc
     6841 gatggatagc aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa
     6901 tatgagtatg attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt
     6961 gttagggcct actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag
     7021 agaactagca gctaaacttg ctgattacta ccgttcgctt gacttgtcaa actcgtaatg
     7081 tgaataacga agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca
     7141 gctgactgaa taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa
     7201 agaaatgaca gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg
     7261 tatttcagaa gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga
     7321 aatggaagat aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa
     7381 aaatgagcgt gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc
     7441 atcaattgac aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg
     7501 tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg
     7561 aattgaaaaa atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca
     7621 aactgttcca gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg
     7681 ctacaaatta catgatcgcg tgttaagacc aaccatggtc attgttgcac aataattgtg
     7741 cggatataaa acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc
     7801 ggtattgact taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa
     7861 atcatcgcta acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga
     7921 gaaatccaag taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca
     7981 tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat
     8041 acaccacaag aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat
     8101 ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa
     8161 cgtcaagcaa caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat
     8221 gaaccaactg cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc
     8281 ttagtattcg accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt
     8341 gtctttgatg tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac
     8401 aaaatcatcg actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa
     8461 gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct
     8521 ggcgtatcaa gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg
     8581 catttggaaa tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa
     8641 cgtacaaaag taccagtacg tcaagcgcta aaagatgcag gtttatcaca atcagaaatc
     8701 gacgaagtta ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt
     8761 aaagaaacag gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt
     8821 gctgctatcc aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt
     8881 acaccattgt cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc
     8941 aatacaacga ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca
     9001 gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta
     9061 ggaagattcc aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta
     9121 acattcgata ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa
     9181 aaagaacaaa aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt
     9241 atggtaaaag atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac
     9301 ctacgtaatg atgtagatgc attgctattc tctgtggaca aaacattgaa agaattagaa
     9361 ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc
     9421 gctgttgaag caaacaacat cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc
     9481 gttcaaaact tgactgttaa actatacgaa caagcggcac aacaacaagc tcaagaaaac
     9541 ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat
     9601 ggcgacgata aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc
     9661 tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga
     9721 taacaagcga cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt
     9781 gattattatg aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct
     9841 tatcgtaagc tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa
     9901 aaattcaaag aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca
     9961 tatgaccaat acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc
    10021 ggatttggtg gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc
    10081 gactctttct ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct
    10141 gatttgcaat atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa
    10201 atcaagtata atcgggaaga aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc
    10261 acacaaccaa ctacttgtca taaatgtcat ggtgcaggta cgattaatgt agaacgccaa
    10321 acaccacttg gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa
    10381 gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca
    10441 gttaaagtga atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa
    10501 ggagaagctg gagaaaacgg cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa
    10561 gaaagtgata tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt
    10621 gtacaagcag ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg
    10681 aagatccctg ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg
    10741 aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa
    10801 aatctaaatg aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa
    10861 gttgaagaac agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa
    10921 aagcggaagt agtttttcta gtaaaaacgc taagtcatta ataattaaaa accagacgaa
    10981 caatcatatg ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag
    11041 ctttagaata tgataatcgt catagtttct aggggtataa ccagtgagag aatggaactt
    11101 gttcaatcga tattgcaagg tgtttttatg aataaataat tcttctgagc aatttttgat
    11161 gcttccgtta tttttactga agcatgccaa agtcttcttg aacaaatgta cttcttcttc
    11221 tgtcaggctt ttcaaaatac gttttacata taatgttcgt tgatctgacg ggattgctgg
    11281 taaaacgatc ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag attgatggaa
    11341 agctatccat tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct cttgctctgt
    11401 ataacaaagc gacccaattc caaagtttag ctgtaactga tacattttat taaactttgt
    11461 ctggatttta tcaagcaatg atagcaatga ggctctggaa atccgattga ctaggataat
    11521 gatttgttgt ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag
    11581 caactccaat aaaggattga tccgttcttg acacaattgc tgattgtcag aaagttgtcc
    11641 cacaactaca taatatggag aagaaaaatc aagctcaatt aagtgagggt ggtatcgact
    11701 atgtacttga tatcatcaat gaaccgatgg tactttctta tgcgatggaa caggtagagc
    11761 gattgcttgt ttgtgcagga aaacaagcga tgcggctgat acgattaggt gagaaaataa
    11821 gaagataaaa atacacggtc ccttcatggc gaccgtgcat ttttggtttt tattaaatat
    11881 ttaatacttt attcaagaaa tcttttgtcc gttcattttg cggattttca aagatttgtt
    11941 gaggcgttcc atcttctaaa aagtttccgt cgtcaatgaa catgacgcga ttggccactt
    12001 ctttagcgaa ccccatttcg tgcgtaacga taaccatcgt cataccttgt tctgctaact
    12061 ttttcatgac gttcaagaca tcaccgacca tttcaggatc gagtgcagaa gtaggctcat
    12121 caaaaagcat gatgtcaggg ttcatcgcta gagcacgagc aatagccaca cgctgttttt
    12181 gtccaccaga aagcatttca ggatacatat cttttttatc ttctagacca actgtctgca
    12241 gtaatttgat tgcttttttc tctgcttctt ctctagattc tttcttcagg tccatcggtg
    12301 ctaaaatgat gttttccaaa atagaaagat gagggaatag gttaaagtgt tggaatacca
    12361 ttccgatatg ttgtctcacc aagttgatat ctgtgctttt atccatcaag tgggcgccgt
    12421 caataataat ttcaccgtca gaaggttctt ctaaacgatt caagcaacgc aagaatgtac
    12481 tttttcctga acctgaaggg ccaatgatac agacgacatc tccttcgttg atggatacat
    12541 tgatgtcgtt taatactgtg ttatcgccgt attttttgac taaatgctca actaagattt
    12601 tttctgccat cttacttcac cttcttttct aaaacttttg ctaattttgt caatgctgtg
    12661 atcaaaatca agtacatgat cgcaatgatc aagtaaacat acgtactttg tgttgtacgt
    12721 gcaacaatga tcttaccagt ttgcagcaat tcgactacac cgattgcgga aatgatcgtt
    12781 gtgtctttta atgagataac gaactgattg acgaatgatg ggatcatgat gcgtatcgct
    12841 tgcggcaaaa tgattttttg cattgtccga ttataagaaa gacctaaact tctggaagct
    12901 tccatttgtc cgacaggaac ggcgttgatc ccgcctcgaa caatctctgc gatataagca
    12961 cttgcgttaa gagtcaaggt gataacacca gcaaggaagt caggaatcgt aaatccaacg
    13021 ataccaggca acccaaagaa aataaagaac gcaagaacca tcattggaat tcctcggatg
    13081 atatcgacat aaatactagc aattgtgcga agagcgcgta ttggtgctac actgaataat
    13141 ccaaaaatca caccgattac tatagccaaa gcaaaggaga tcaaagttag gagaattgtt
    13201 ctccaaagtc ctttcaacaa tgcttgatag ttgttttgga taagtccaag aaatgttgat
    13261 tcatcagcag tggaagactc actggaagtt tctgaaccat ccgctacata ttctttaata
    13321 atcttatcgt attcgcctgt tctttttaat tctttcaatc cttcattgaa catattaaga
    13381 agctcaggat tttgtccttt tttaacggca aatgcatatt ttccgccaga ttcttttttg
    13441 atcggtgttt ccaattcttg tccttgtttg atcgcataac cgattaccgg ataatcatcc
    13501 atcattgcat cgattgcgcc aacttttaac ccgtcgtaca attgatcagc agcatcaaaa
    13561 tatttgatag aatagccata ttcagattca tggtcgctta aaaattcagc actttcggta
    13621 ccaacttttg ctccgactgt ttttcctttc aaatcttcat aagacttgat cgaagtatct
    13681 ccttttttga cagctaattg gatcccgcta tcaaagtaag aatcagaaaa atcatagctt
    13741 ttctttcgtt catctgttac agtcattccg gcaatcattc cgtctgcttg tcctgattcc
    13801 aaagcttgca aagcagcaga gaaaccacgg tagtccattc ggatgttaaa tccttgcagt
    13861 ttggcgatgt tttccatcaa ttttacatcg atgccttcgt actcgccctt tgcgttttga
    13921 aattcaaatg gagcaaaagc agagtcactg gcaattacgt atacgtcttt tttgggctct
    13981 attttttgca ttgtctcgct tgtgcttgac tctgccgttg tagtgttgtc agcttgtgca
    14041 acatgaccaa acgcaagtgt cacaagtcct gtcattaaga aaacaaatga gtgaaataaa
    14101 tgctttcttt tcataatcaa cctcctaaat ttccaatatt ttattattat aggcgaaaag
    14161 tgctgaatca gacaagactg taagctcgaa aaaatgaaaa aaatgcgagt ttctaacatt
    14221 ttatgttata aaacataaca tgtataataa gataaaaaag tggcgaaagt atgttcggtg
    14281 tggtataatt ttttagtaag gaatgccttt gctttccttt tctttttatt ggtcgtaaaa
    14341 tgaaaaggaa tgaatgaaaa ggaggacgaa gaatgattga gagagatacc tcccgacact
    14401 ttgagcttgt ctcgaaatac caaccggctg gtgatcagcc tgaagcgatt aaccaattag
    14461 tggatggcgt agtcggcggg aaaaaagcac aaatcttatt aggtgcgact ggaacaggga
    14521 aaacatatac gatttccaat cttattgaga aagtgaataa accaacactg attattgccc
    14581 ataacaaaac attggctggt cagctgtacg gggaattcaa ggaatttttc cctaacaatg
    14641 ctgttgaata ttttgtttct tattatgatt attatcagcc tgaagcttat gtgccttcaa
    14701 gcgatacgta tatcgaaaaa gattcaagtg taaatgatga gatcgacaaa ttacgacatt
    14761 cggctactag ttcattatta gaaagaaatg atgtgatcgt cattgcatcc gtttcatgta
    14821 tttttggatt aggctcgcct tttgagtacc aaaaacaagt agtttcgatt cgacagggag
    14881 cagaactaga tcgcaatcag ttgattcgtg acttagtcag tatccaattc gaaagaaatg
    14941 atatagattt tcagcgcggt cgttttcgag tgcgcggtga tgtcgtggag atttttccgg
    15001 cttcaagaga tgaacgtgcc ttacgtgtgg agttttttgg agatgaggtc gaacgtatcc
    15061 gagaagtcaa tgcactgaca ggtgaagttt taggagaaac ggaacacgta gcaatcttcc
    15121 cggcgactca ctttgtgacg aatgatgaac atatggagca tgccgtagcg aatatcaaag
    15181 cggaattaga acagcgttta actgtcttgc gaaatgaaaa caaacttctg gaagctcaac
    15241 gtttagaaca gcgaaccaat tacgatattg aaatgatgtt agaaatgggc tacacgtcgg
    15301 gaattgaaaa ttattctcgg catatggacg gacgtaaaga aggtgaaccg ccttatacgc
    15361 tattggattt ctttcctgaa gattttttga tcgtggcgga cgaatcgcac gtaaccatgc
    15421 ctcaaatcag aggaatgtat aatggtgacc gagcgagaaa acagatgcta gtagattacg
    15481 gtttccgttt gccatctgct ttagacaatc ggccacttcg gttagaagag tttgaaaaac
    15541 atgtcaatca gatcatttat gtctctgcga cgccaggacc atatgaacat gaacaaacag
    15601 ataccgttat ccaacaaatc atccgtccga cagggctgct tgacccagtg atcgaagttc
    15661 gtccaatcat gggacaaatc gatgatctag taggcgaaat aaatgaacga gtcgaaaaag
    15721 accagcgggt gtttgtaacg acattgacga aaaaaatggc agaagacctc acagattatt
    15781 tcaaagaatt agggatcaaa gtcaaatatt tgcatagtga tatcaaaacg ctggaacgaa
    15841 cagaaatcat tcgagatctt cgattaggtg aatttgatgt gttagttgga atcaatttgc
    15901 ttcgtgaagg attagacgta cctgaagttt cccttgttgc tatcttggat gcggataaag
    15961 aaggcttttt acgtagtgaa cgttccttag tccaaacgat tggacgagct gctcggaatg
    16021 aagaaggtaa agtaatcatg tacgcagaca aagtaacaga ttctatgcgt ctagcgatgg
    16081 atgaaacgtc aagacgtcgt acgattcagc agaagtacaa tgaagaacat gggatcgttc
    16141 caaaaacgat cattaaagaa atccgagatt tgatttctat tacgaaagaa tcggaagatg
    16201 atacaaaaga agcggtacaa gtctcgtatg aagaaatgac gaaagaagaa aaagatacgt
    16261 tattgatgaa attagaaaaa gaaatgaaaa atgcggcgaa agcattggat tttgaaacag
    16321 ccgctaatgt gcgagatatg attttagaat taaaagcttc aaagtaggag gacaacatgg
    16381 ctaacgataa aattacgatt cacggtgcac gtgcccataa cttgaaaaat atagatgtaa
    16441 cgattccacg agataaattc gtggtcgtta caggtttgtc cggttctgga aaaagttccc
    16501 tagcatttga tacgctgtac gctgaagggc agcggagata cgtagagagc ctttctgctt
    16561 atgctcgtca gtttttagga caaatggata agccggacgt agatagcatc gacggattga
    16621 gtccggcgat ttctattgat cagaaaacaa caagtaaaaa tcctcggtcg acagtaggta
    16681 ctgtgacgga aatcaatgac tatttacgct tgttgtttgc acgtgtagga catccgattt
    16741 gtcccaacga tcatatcgag attaccagtc aatcagtaga tcaaatggca gatcaagtaa
    16801 tggctttgcc tgagcgcaca aagatccaag tgttggcacc gatcgtggta aagaaaaaag
    16861 gacaacataa aaaaatattc gaacgaatcc aaaaagaggg atacgtccgc gttcgagtcg
    16921 atggtgagat ctatgattta agtgaagtgc ctgagctgga gaaaaacaaa aaacatgata
    16981 tcgcgatcgt catcgaccgg atcattgtga aagaagggat tcgttctcgt ctatttgatt
    17041 ctttagaggc ggctctgcga ttggcagacg gctatgctct ggttgacgtg atcggtgaag
    17101 aagaaatgtt gtttagcgaa cactatgcct gtccttattg cggatttact gttggcgaac
    17161 tagaaccaag actgttctca ttcaatgcgc cttttggagc ttgtccagac tgtgatggtt
    17221 taggtgtcaa attagaagtc gatcaagatt tagtgattcc agatagaacg aagacactta
    17281 gtcaaggtgc tatcgttcca tggaatccaa tcagctctca gtattatcct caaatgttag
    17341 cgcaggcttg tgaaagtttc ggtatcgatt tagatacacc attcgaagaa ttaccggaag
    17401 agcatcagga tatcatcttg aatgggacag atgatctttt ccatttccat tatgaaaatg
    17461 attttggtgg cgttcgcgat gtagaaacga ctttcgaagg ggtattgaag aatatcgagc
    17521 gtcgttatca tgatacgaac agtgatttca cgcgtgagca aatgcgtctt tatatgacag
    17581 aattaacttg tcagacatgt aaaggttatc gattgaatcc ccaagctcta tctgttcaaa
    17641 tcaatggaac gaatatcggg caaacaaatg agttgtctat tcaaaaagca gtagatttct
    17701 tctcaaactt aacgttatct gaacaagaga aagtaattgc caaaccgatt ttaaaagaag
    17761 taaatgacag actttcattc ttggaaaatg tgggactaga ctatctaact ttgagtcgcg
    17821 cttccggaac attatctggg ggagaagcgc agcgaatcag attggctaca caaattggat
    17881 cgaatctttc tggagttttg tatattttag atgaaccatc gatcggattg catcaacgcg
    17941 acaacgatcg actgattggc tcattaaaga aaatgcgtga tcttggcaac accctcgttg
    18001 tagtcgaaca tgatgaagat acgatgtggg cagcagatta tctgattgac gtcggacctg
    18061 gggccggaga acaaggcgga caaatcgtgg ctgcaggcac tcctgaagaa gtagctaatg
    18121 atgaaaattc tttgacaggg aaatatcttt cagggaaaaa atcaatccct gtaccaaaag
    18181 aacgccgtaa aggtaatggg aaagcaatca agatcactgg tgcttcagaa aacaatctga
    18241 aaaatattag tgttgaattt ccattaggag aatttgtagc agtcacaggt gtgtctggtt
    18301 ctggaaagag tacattggtc aatagtatct taaagaaatc gttagcgcaa aaattaaata
    18361 agaattctgc taagccaggt aaattcaaga caatttccgg ctacgaaagt atcgaaaaga
    18421 tcatcgatat cgatcaaagc ccaatcggcc ggacgccgag aagtaatcca gcgacttata
    18481 caagtgtatt tgatgatatc cgtgggttat ttgctcaaac gaacgaggca aaaatgcggg
    18541 gttataagaa agggcgtttt agtttcaacg taaaaggcgg tcgttgtgaa gcttgtcgcg
    18601 gggatggaat tattaagatc gaaatgcact ttttgcctga tgtctatgtt ccttgtgaag
    18661 tatgtcatgg caaacgatat aactctgaaa cattagaagt gcattacaaa ggaaaaagca
    18721 ttgctgatat tttggaaatg acagtagaag atgctgtaga attcttcaag cacattccaa
    18781 agattcatcg caaactgcaa acgattgttg atgttggctt aggttatgtg acgatggggc
    18841 aaccagcaac gacattgtcc ggtggtgagg cacaacggat gaaacttgcc agtgaattgc
    18901 acaaaatctc taatggaaag aatttctata tactagatga accaacgaca ggacttcata
    18961 gcgatgacat cgcccgcttg ttgcatgtat tacaaagatt agtagatgct ggtaacacag
    19021 ttttagtgat tgaacacaat ctagatgtaa tcaaaacagc agattatatc attgatttag
    19081 gaccagaagg tggagaaggt ggaggaacga tccttgcgac tggaacacca gaagaaatca
    19141 ttaacgtaaa agaaagttat acaggtcact atttgaaaaa aataatggta taaagaggat
    19201 agatagctga gatagttaaa aaaacataaa aaaatgaatt tttgcttgtt ttattaatat
    19261 cagtgttcta gaatcattac atggtagttc tttctacact gattggtata gacaaaaact
    19321 tcctataagt tcaatccgtt caaaaaagaa ttgagcaatc ggaggagttt cctttgattg
    19381 cttttttctt tatggtatga tattccttgt gtagttggaa aagaaaaaca agagattatg
    19441 agtaagtgag agataatgat gaacagtatt gaaaatgaag aatttatccc tatattatta
    19501 ggaagcgaca tgaacgttta tggtatggca cgttctttta atgaagcata cggcaaaatc
    19561 tgccaagcat atgcttcgga tcagttagcc cctacccgct acagtaaaat tgtcaatgta
    19621 gaagttattc caggatttga taaagaccct gtgtttatcg agacgatgct tcgtttggca
    19681 aaagaaagat attctgataa gagtaaaaaa tatcttttga ttgcttgtgg tgatggatat
    19741 gcagaattga tctcccaaca taaacaagag ctgtcagaat attttatctg tccatatatt
    19801 gattattctt tatttgaaag actgatcaat aaagtaagct tttatgaagt ttgtgaagaa
    19861 tatgatcttc cgtatcctaa aacattgatc gtcagagaag aaatgttagt aaatggacat
    19921 ttggaacagg aattgccttt tgaattccca gttgctttga aacctgcaaa cagtgtggag
    19981 tatctttctg tccaatttga aggaagaaaa aaagctttta ttttagagac tagagaagag
    20041 ttcgatttaa ttttaggccg gatatacgaa gccggttata aaagtgaaat gattgtacaa
    20101 gattttatcc cgggtgatga tagtaatatg cgtgtgctaa atgcgtatgt agatgaagat
    20161 catcaagttc ggatgatgtg tcttggtcat ccactgttag aagatcctac accagcatcg
    20221 attggtaatt acgttgtgat catgccagat tataacgaaa aaatctatca aaccattaag
    20281 gcatttttgg aaaaaatcga atatacaggc tttgccaatt tcgatatgaa gtatgatcct
    20341 agagatggag aatataaatt atttgaaatc aatttgagac aaggtcgtag cagtttcttt
    20401 gtgacattga acggcttgaa tctggctcgt ttcgtcacag aagatcgagt gtttaacaaa
    20461 ccctttgttg aaacgaccta tggaacgaat caatcagata aagcaagatt atggatgggt
    20521 gtgcccaaaa aaatcttttt agaatatgct cgggaaaatg aagacaaaaa actagctgag
    20581 caaatgatca aagaaaatcg ttacggtact actgtgtttt atgaaaaaga ccggtcaatc
    20641 aagcgctggt tgttgatgaa atatatgttc cataactata ttccaagatt caaaaagtat
    20701 tttcatgtga aagaaggcta gttcatggag aattttttca gtattttagg cggaatgggc
    20761 acgatggcga cagaaagttt tgttcgcttg atcaaccacc gaacgaaagc tacaaaagat
    20821 caagaatatt tgaattatgt cttattcaat catgcaacag ttcctgatcg tacagcttat
    20881 attttagatc gatcagaaga aaatccaatg ccgtttctgc tggatgatat tgagaaacaa
    20941 aatttattgc agccgaactt cattgtgttg acttgtaaca ccgcacacta cttttttgaa
    21001 gaattacaag cggcaacgga tattcctatc cttcatatgc caagagaagc agcaaatgaa
    21061 cttgtccgtc agcatacgac agggagagta gcgattctag gaacagaagg aagtatgaaa
    21121 gctggaatct atgaacgcga agtcaaaaat cttggattcg aaacagtgat tcctgatact
    21181 gttcttcaag aaaaaatcaa ttatctgatc tatcatgaga tcaaggaatc ggattatttg
    21241 aatcaagagc tatattacga aatcttagaa gaagcggtag agcgcctaaa ctgtgaaaaa
    21301 gttatcttag gttgcacaga attgtcattg atgcatgaat ttgctgaaga taaccattat
    21361 ccagtgatcg atgcccaatc tattttagca gatcggacga ttgaacgagc gctagctgaa
    21421 agaagcaagg cattggatac agcatcggaa aagtaaaaaa attggatttg gggcaaatat
    21481 cctaaaagat atttggtcca aatctttttg taaaagaaaa agaaaactgg taatgatacg
    21541 cccggaatga tataatttta ctatgcacaa tgtgagagga gttcgtaatt atgacagata
    21601 atttgcaatt agtcatcata acaggtatga gcggtgcagg gaaaacagta gctatccaaa
    21661 gttttgaaga tatgggctat ttttgtatcg ataatatgcc gcctcgattg attccaaaat
    21721 tctgggaatt aatcaaagaa tcaggcaaag tcacaaaaat ggctttagga gtggatttac
    21781 gttctcgttc cttctttgaa gaaatccaaa acatgctgat cgaaatcgaa aatacgaact
    21841 tcatcgacac ttctatctta tttttagatg cttcagacga agaactagtg gctcgctaca
    21901 aagaaacaag acgagcacat ccaatggcga tggacggttt gatcactgaa gggatacgta
    21961 aagaacgggc gatactagac gacttgaaag cccaagcttc cgtgatcatt gatacgacga
    22021 cattgacacc acgacaatta cgtgaaaaaa tcaatgagtc attcaaatca aagggagacc
    22081 aaggatttcg gatcgaattt gtatcatttg gttttaaata cgggttgccg atcgattcgg
    22141 atattgtgat ggatgtgcgt tttttgccca accctcatta tatcccggaa ctaagaccat
    22201 tgaccggaaa agatcagcca gtctatgatt atgtgatgtc ctttgatgaa acgcaatcat
    22261 tctaccataa atttttggat cttctggaaa ctgtattgcc tggatacatt aaagaaggaa
    22321 aaagcagctt gacagttgca atcggctgta caggcggtca acaccgttct gtggcattga
    22381 ctgaacgttt ggcaaatgct ttagggaaaa actataaagt caatgtgaca catcgggaca
    22441 aggataaaag gaaagagacg gtgaaccggt cgtgaaaatg aaaacctacc ggataagaaa
    22501 accaaagatc gtagtggtcg gcggtggcac tggtctgcca gttatcctga aaagtttgcg
    22561 aaatcaagga gcagatatca cagcagtagt gactgttgca gatgatgggg gaagcagcgg
    22621 tgcaatcaga gaatcgatcg ctatggctcc tcctggtgac ttgagaaatg tcttagtggc
    22681 gttatcggat atgccacaat tctatgaaga tattttccaa taccgtttca aaaaggaaga
    22741 tcagttttta gccaatcata cgatcggtaa tctgatcatt gccgcagttt ccgagatgcg
    22801 gggaagcact tatgaggcga ttcaattatt agcaaaaatg atgcatgtag atggacatgt
    22861 ttatccttct tcagaaactc ctttgacctt gcatgctgtc tttaaagacg gaacgactgc
    22921 agtaggagaa tctaaaatcg cgatcgaccg taaaaccatt gaccgtgtgt atgtctgcaa
    22981 cacaaatgat aaaagtgaag ccaaagcggc tcgaaaagtc gtttcagcaa ttatggacgc
    23041 cgacatggtc gttttgggac ccggaagttt attcaccagt atccttccaa atttagtgat
    23101 accagaaatc ggcgaagcaa tgctcaagac aactgctgaa accgtctata tctgtaatat
    23161 catgacccaa aaaggggaaa cggaacattt cacagatgcc gatcatatac gtgttttaca
    23221 caagcatttg aatcagcctt ttatcgatac agtattggtc aatacggaaa aagtgcctga
    23281 agattatatg gacccagaga tctatgatga gtatctagta caagtcgcac acgacttcaa
    23341 cggattgaga aatgaaggat gtcgagtgat ttccaccgac tttttggaat tgaaagatgg
    23401 tggtgtcttc cacgatggag acaaagtagt agaagaattg ttccgtttag ttttcggttc
    23461 aaaatattaa gcaatttatt gttggagaaa ggaggagaat aggatgtcat ttgctgcaga
    23521 tgtgaagaaa gaactaactg gtttggaagt tcatcgagaa catgcgaaag cggaattagc
    23581 tgctttgctg cgaatgaacg gctctttagg tattgtgaac cagcagttta ttttgaacgt
    23641 ccaaacagaa aatgctgcaa ttgccagacg tatctattct cttttgaaag atcattatca
    23701 ggtacgaagt gagctactcg ttcgtaaaaa aatgaaatta aaaaagaaca atgtctatat
    23761 cgtccggttg aagcaaggca caaaagatgt gttgatggat cttgatatca tggatggtat
    23821 gatgttcaat ggacacgttt cggacgagat tatgggaaat tcgcaaaaaa tgcgttctta
    23881 cttacgtggt gcttttatgg cctcaggttc agtcaataat ccggaaacga gccgttatca
    23941 tttggaaata ttttctattt atgaacagca taaccaagac atttgcgata tgttaaatta
    24001 ttacggtcta catgctcgaa cgcttgaacg gcgcaatggc tatatttctt atttaaaagg
    24061 agcagaaaaa attgctgatt tcttaacatt gattggtgca accaactcaa tgctccgatt
    24121 tgaagatgtt cgaatcgtac gggatatgag aaattcggtc aatcgtctag tcaattgtga
    24181 gacagcaaat ctgaacaaga cgattgatgc ggcttctaaa caaatcgaaa acatcgaatt
    24241 gatcgaagca aaggtcggtc tacatgccct tcctgaaaaa cttcaggaaa tcgcagaact
    24301 tcggcttcaa catccagagg tcagcttgaa agaattaggg gaaatgattc cctcaggtgc
    24361 gatatcaaaa tcgggaatta atcaccgaat tcgcaaaata aatgaatttg cggaaaaact
    24421 gcgtgaagaa gcagtttaat ccaaagaaaa aactgataca tgaagcagga gatgcgaatg
    24481 tatcagtttt ttttaaaagt tatacttgct tttcttccgt caaactataa ttccatcttt
    24541 actggagtaa cataatttgg aaaggcttct attgttttta atacaatcgt ataaaaagcg
    24601 gggagacaac tgaatgaaga ttttagtttt gtacataaat ttttgcttaa tatagaagtc
    24661 ctgatataga taagatcaag tatttatcta taactggcct gattaattaa aaaactggaa
    24721 ggaactgctc gatcatcctt gagaactgct cttcttactt ctggtcaaga ttctcgtggt
    24781 tggtggataa ggatggttga taagttcatt ggattagcta gtcgaaaaca accaggtgta
    24841 attaatgtag aagttttaac aaggggatgg agtgatttat tcagtgataa tcataagttc
    24901 ataaatagta attgagatgt atggttggaa tacgttatac ctgaacttga actaccaaga
    24961 tttggtttag aatttgagaa tcgatgttta taaagggagg gcaaaaatgc gtttttttac
    25021 aacttttact atcattatga ttgcagtact ttttattttt ctagatatag caaagcgaaa
    25081 tacagcattt ttattatatc gggttttact tcgagcaggt ctcataacat ttatatcgat
    25141 cgttggattt tttctattca cagttattgt atttatttgg agaactccag caccaccatt
    25201 gccagagatt acatatggag aatttccttt tagacttgag tatgaattga atgaagaatt
    25261 acatgtgata gaagacacgt taatagttga atttgatgga tttggaatga acgaaggcat
    25321 agggagatat cgtagatgga caagtcgttt agccagcgga gaagatttag tgttgctgtt
    25381 agaagttagt gataataagc aaatatttta ttttccagga cctgctaatt attacatggg
    25441 agataggttg aatggttata atcatacttt tcctagtgct tctttcattg aaagggaaag
    25501 aggcattact agaagggata ttttacatga caaagaatta ttagaacaat ttggtccgct
    25561 tgatcaaaat acaataaatg aagaagaatt gttaaatcaa tataatataa ggctagtgaa
    25621 ttgggaaata tctgaaccga ttgttaacaa ttttggtgat tagaaaacaa ttacgataaa
    25681 tagaaacaag ccaaatcatc agcaagtaaa agctgatgat ttggcctttc ttatacgagt
    25741 agtaggaatt catagcgaat attattacga tcagtagata ttttgatatt aaaggagtat
    25801 tacgaactag tatgtgagaa tgtagaggtg atttgcgaat aatgagaaat ttatcaaata
    25861 gaaacaaaat attaatcatt attgttgtta tagctgtgtt tcatctggga acgaatgcgg
    25921 ttttaagtag aataatattg ggtcctaagc caccaagacc tgcgattacc cgaggggagt
    25981 ttgattttag attagaatat gaagtagatg gggaaaggat tgtgatagaa gatacaattg
    26041 ttgcattatt tgatggattt tctgcagatg caggtagcat ggcatggtat cgcacgtggc
    26101 gtttgcattt ggcgagtgac aagagggcac aacaattatt gttacatgag ttagatgatg
    26161 gtcgaaaaat atattatcat tttggaggag ctggatttta tatggggaag ttagtagcag
    26221 tgtaactgta acaaggaaag atttgccagg tcttggcatt cgaaactcca tattttatat
    26281 cgagcgtggg agagatacat ctattgggaa caggaaaggg ttaactcttg aagagttata
    26341 taatgacttt ggaatccgtc ttgttagttg ggaacacaat ccaccaatag aaaatcgctt
    26401 tgaataatcg aaatctatta atccatttca tcagccagtg aatgttgtaa tggaacaatt
    26461 tggtttagtg ttcaaaacca ttgaaatact aggctgtgat cagcaaaaat gtatctgaat
    26521 aaaaaagaga gcaactatta aatgagagga gttaggagtt gttagcttta tacgaatcaa
    26581 agtggcaaat acagatgcgt atgcatttgt gtttgccatc ttgcgtttgg tagacgagat
    26641 aggtataagc taaagaaaca acttgtttaa aataaacgac ctagctcaac aaacgaccat
    26701 ctaatcaact aacagcatac ataaaagcaa ctatttttga agtagaattg aaatcgggat
    26761 aacttaaaaa gtgattggga tactagtgtg aatcaccgta cataaatatt actcgttaag
    26821 ggaatttgag aaattttgta tgaaaattta agaattagtt agtatgcgca agtcaaaaat
    26881 atgaataaat tatttggtgg agataatttg ctcagcttgt cataagaaat gagataatct
    26941 ggcagatgca agcttttgga caccatttat tcggaataag gaggctggga cagaagcgtt
    27001 taactccaag aaataagaag gagttgcttc tgcttccgcc gtttataggt gtttagagcg
    27061 tgagacaaaa gtgttcttta cttttgtcct acgctcctta tttttttatt ttgtttggat
    27121 caattctttt aattcctcta ttgaaataga ttcatcaaag atttctttac ccaagatgac
    27181 agtcggaaca aatcgaatat gtgcttgctc agcttcgttg atgacagctt ggctcagctg
    27241 ttcgtctttt tgttctgtca gctttagttt atctactgca aaatccgcta cttcttggag
    27301 agaaagatgt ttccactcgt cttgagtatc gaatatttgt ttgatctctt tgatcgcttt
    27361 ttgaccatcg ctgattgtga gataccggtg catcacgttt cctcgctgca agctttcttt
    27421 ttctttatcg aacaatttga tcactcgctg caattgcccg ctttggactg cttcttctaa
    27481 tagctcataa gattcttcaa accattgtcg gcaatacgga caagataggt tgatgaattc
    27541 gaccattttt ttaggagcat tcgatgaacc ataaaggatt cctttttgtg tatttgtttt
    27601 cgttgcatca atcactgata tatccatgtt aaaacacctc tttcgcttat agtttataca
    27661 tgtctgacaa aaaaaggcaa cgatttagac tgtttgtcta aaaattagat gaccaattca
    27721 aatagacata tgtgaaatat ttttataaat aaaagaaaaa atggaatgtt ttacgttata
    27781 atggaacaga taataagaga gaatatgaac aaggatgatg aatcatgcga aaaatgaaaa
    27841 caatggatgg caatacggca gccgcatata tttcttatgc atttaccgag attgctgcaa
    27901 tatatccaat aacacccagt tcgaccatgg ctgaattagt agatcaatgg tcagcagcag
    27961 gaaaagaaaa tgtattcggt caaccagtaa aagtagtaga aatgcaatcc gaagcaggag
    28021 cagctggtgt ggtccatgga tcattgaaaa ctggtgcact gacaactaca tataccgctt
    28081 ctcaagggct gcttttgatg ataccgaata tgtacaagat tgctggggaa ctactgccaa
    28141 gtgttttcca tgttgctagt cgggcagtga cgacaaatgc gttgaatatt ttcggagatc
    28201 acggagacgt gatggctgca agacaaacag gatttgctat gttagctgaa agcagtgtac
    28261 aagaagtgat ggatttagca cctgttgccc atcttgcttc tattgaagga gaagtacctt
    28321 ttttgaattt ctttgatgga ttccgtacga gtcacgagat tcaaaaaatc gaagttttgg
    28381 aatatgaaga attagctcct ctgatcaatc aggagaaatt agcttcattt cgtaaacgag
    28441 ggatgaatcc caatcatccg actgttagtg gtacgaacca gaatccagac atccattttc
    28501 aacaacggga aacgatcaac ccctattatg ataaattacc tgaaatcgtt caaaaatata
    28561 tgaaagagat caaccgatta cgaggaactt cttatgactt ggtgacttat tacggagcag
    28621 acgatgcgga agaagtcatt gtttctatgg gatcagttgc acagacgatt gaacaaacga
    28681 ttgattactt gaatgcaaac ggacggaaaa caggtttttt gaatatccat ctttatcgtc
    28741 cttttccagt ggaaacattc gtgcaaaagt tgcctcatac agtaaaaaca attgctgttc
    28801 ttgatcgaag taaagaacca ggggcaggtg gagaacctct cttattagac gttcaaagtg
    28861 cgttatatga ttctgaactt cgcccggctg ttattggtgg tcgctacgga ttggggtcta
    28921 aagatgtgac tcccgaccaa atctcagcag tttttgatga attgaaaaaa gacccaagca
    28981 tacgcaaaaa acggtttacc atagggatcg tggatgatgt gacttatcaa tctttggaac
    29041 caaaggaaag tctggatttg acagaaccgc aaacattcca ggccaagttt tggggatttg
    29101 gatctgacgg aacagttgga gcaaataaat cagctatcaa gattattgga gatcacaccg
    29161 ataaatatgc gcaaggctat ttttattatg attcgaaaaa atctggcggt ctgactgtct
    29221 ctcatcttcg ttttggagat acgccgatac gttcagcgta tcttgtcgag catgcggacc
    29281 ttgttgcctg tcacacacca gcttacctgc atagctatga cttggtcaaa ggattgaagc
    29341 caggtggcat ttttttattg aatactcttt ggagcgacga acaattagaa acgcatctgc
    29401 ccttaaaatt aaaacgttac ttggctgaaa ataacattag attctatacg atcaatgcca
    29461 tgagattggc ccaagaagtc ggtttgggac gccggatcaa tacggcaatg gaaacggctt
    29521 tttttaaatt agctgacatc attccttttg atgaggtgct tccgctgcta aaagaagaag
    29581 ccctgaaaag ctatggacat aaatcaatgg ctatcgtaga aaaaaacata caagcaatcg
    29641 atcggacgac cgaactgctt catcaagtcg cgatcccaga ccattggaga acgttggaga
    29701 tgcctgaaaa aatcaataaa aggaacactg gttatataca agaaatactt gaaccagtga
    29761 atgcacagga aggaaatcaa ctctctgttg gtacacttgt agcaaatgga atgacaactg
    29821 gcgaaatgcc tttaggaacg gcttctatgg aaaagcgggg cgtcgcttta gaagtacctg
    29881 agtggattag tgatcgctgt acgatgtgta atgaatgtgc ctttgtttgt cctcatgctg
    29941 ccatccgtcc atttttagca gatgaagagg aaatggaaga agcgccagaa ggatttatcg
    30001 tgagagaaat gcgaggcgca gacggcgtaa aataccgtat ccaagtttca gtggaagact
    30061 gtacaggttg tggtctgtgt gtagaggctt gtcccgcaaa agaaaaagct ttagtgatga
    30121 agccttatga agaacagaaa gaacaagctg tgaattgggc ttttgcaatg acattaagac
    30181 aaaaagcaaa tccagtcaaa ggaaagaata ctgtcttgtc tacacaattc aatcaaccgc
    30241 ttcttgaatt ctcgggcgct tgctcaggct gtggcgaaac accttatgta aaattattga
    30301 cacaaatgtt cggtgatcga atgatgatcg cgaatgcgac aggctgttct tctatttggg
    30361 gcggtgcttc tcctgcttct ccttatacga ccaatgaatg tggtcaaggg cctgcatggt
    30421 caaattcttt attggaagat aatgctgaat ttggttatgg tatgcttcta gcggcacaga
    30481 ctcgtcgcga ggctttggca agttcaatca aagaagtatt gccaaaagta tcagaagaac
    30541 tacgagaatt gatggaagac tggttggtac acatgtttga gagcgaagga acgcagcaaa
    30601 gagcagcaaa actgcaagct gcgatcgaaa gcgaacgatc aattgtccct gaactggagg
    30661 atatatacaa gagaaaggat ctattcgtaa aaaacagcca atggatgatt ggtggagacg
    30721 gatgggctta cgacattggt tttggcggga tcgatcatgt acttgccagc ggagcagacg
    30781 tgaatattct tgttttggat aacgaagtct attccaatac tggcggtcaa acctcaaaag
    30841 caacaccagc ttcggcaatc gctaaatttt cggctagtgg aaagtatgct tcaaagaaag
    30901 atctaggaat gatggcgatg acatatggaa atgtttatgt ggcgcaaatt gcttcaggtg
    30961 ccaatcaaat gcagacgata aaggcttttg aagaagcaga aaagtatcct ggaccttctt
    31021 taatcattgc ctatacgcct tgtatcacac atggtttgct tggtggaatg aaagaatcgc
    31081 ttaaagaagc aaaagaagct gtttcttctg gctattggtc attatatcga tataaccctg
    31141 caatggtcga aaaaaacaag aacccaatga ctttggacta caaaaaacct gatttctctg
    31201 ctatgcctga tttcatgagg aaacaagttc gcttttcctc gctagagaac gcaaatccaa
    31261 atcttgcaaa agctttattt gaaaagacta tccaagacgc taaaacacgt ttttacaact
    31321 atgctgcact gacgggagaa ttagaaaaaa tcaagaaacg acttgaacca gaggaatcta
    31381 tcgatgtacc cgttaagact agaaaagaaa aaacgacgga ccttgaagca gaagaaagaa
    31441 gagctgccag agcagaacga cgtaaaaaaa tggaacaagc tacaaaagaa aaaaaggaaa
    31501 aataacagaa aaaatataat gaataattct ctgaagttta aaaatattta ttgcagagaa
    31561 aaataaaacg catacttgca attttcctga aaattatgcg tttttctcaa gaaaaatacg
    31621 agcagatcag cctctaaaat taaaacatat gatatactag agaggaaatc tcaaaagaga
    31681 agagaggctt tttatgtcat ttcaaatcgg ggaacttttt aatttggact attggcagca
    31741 aatgttttcc ggtgacttgt tctctctgaa ttttttggtg aacatacttg atattctggt
    31801 tgtctggtat cttgtataca aactgatcca actgctgcga ggcacaaaag cgattcagct
    31861 tctgaaaggt gtagctattt ttattgttat ccgaatcgta gcggaaatca ttggtctgca
    31921 caccttgtct tggctgatga accaagtcat tacgtacggg gtgatagctg ctatcgtgat
    31981 ttttcagccg gaaatccgga gaggactgga acatcttgga cgcagttcgc ttttcaaaca
    32041 atcaaaaagc gaacagcagg aagatgagaa gatgatcttg tcttttgaca aagccatcca
    32101 atatatgtcc aagcgaaaaa tcggtgcgct aattactatc gaacggcata ctggattaga
    32161 tgagtatatt gagacaggaa ttgcattaga tgcagatatc actggagagc tgctgatcaa
    32221 tatctttatt cctaatacac cgctgcatga cggtgcagtt attgtaaaag aaggaaagat
    32281 cgcagtagca agcgcctatc ttcctctatc agaaagtatg ttgattccaa aagaattcgg
    32341 gactcgacac cgggcagctg tcggtataag cgaagtcagc gacgcgatta ccatcgttgt
    32401 ctctgaagaa actggggatg tcagcattac attagataat gaactgatgg ctggcttatc
    32461 ccagcaagaa tatttggcaa ttttgcgtag agaactgatt gctgaagaga aaacaaaaaa
    32521 caagaaaaac gtgattcaat ctttcctaga cggtatgact aagggagggg atcgcaaatg
    32581 atctcaaaag agaaacggac aagcattctt tacagtttta ttgcattgct gttcagtatt
    32641 gcgctgtact tcaatgcaaa tggccagtcc gttccaaata cgctttctgg aaatgaagcg
    32701 tataatcaaa cagttactgg ggtgccaatc aagatttctt atgattcaaa gcgctactat
    32761 atccatggat atgaaaatgc agtgactgtc aaactttcca gtgcaaaccg agttcagttg
    32821 aatgcagagg caaacgaaga tacacggatg ttccgcgtca cagcagatat cactaagctg
    32881 ggcgaaggaa cacatgaggt tccgctaaaa attcaaaatt tgagcagtgc tgtcacagca
    32941 aagatcgaac catcgactat tacagtaacg gttgagaaaa aggtaataaa agatttcgat
    33001 gtggaaacgt tgattccttc aacgttcaca ccatctggat atgaacttga tgatacttcc
    33061 gttagtccta aaaaagtctc tatcactaca ggtgacaaaa cattggcaga aatcaaacga
    33121 gtagtagctg atgtggacgc ttcgatggtc acagatgatg ggattaatag tgaggtccca
    33181 atacaggcgc tgaatgcagc aggggaagta ttaagtatcg tctctgatcc agtgcaagta
    33241 acagtgagag ctgattcgat caagccaagc aagtcggtac gactttatgg gatacagcaa
    33301 ggaacaccag ctgccggggt agaaagctat gatttcagtt tttcaaaact ggaagcagaa
    33361 gtaagcggct ctagcgattt gcttgcatcg atcggtgaca gtattgctgt accgatcaat
    33421 gtctcaggta tcaatcatcg aacaactcgt aaaattgaaa taccagtaga agaggggtta
    33481 agtatcaagc ccgaatcagt tagtgtggag attacacccg tgttgcaaac gtcaagcagt
    33541 agctctgctt cgacgagcag tcggacacaa gaaaatacaa gtacgagtag aagtatttca
    33601 tctgctgatt caggaacgga accatcgaca acaaccattt cttccagttc ggaatctggg
    33661 acaagcagct cgaccacaag tacagaagaa acatcagaaa caagcgaatc aacacaaatg
    33721 agcagaacaa attcctcaga aaattaagtt aaaggagtaa ttttacaact atgggtaaat
    33781 attttggaac agacggcgta cgcggtgaag ccaacaagga acttacgcca gaacttgcat
    33841 ttaagctggg acggtgcgga ggttatgtcc taagccagca tgaagaagaa ggaaaacgcc
    33901 cacgcgtgtt agtagggcgg gatacaagaa tctcaggtca aatgttagaa gaagcgctga
    33961 tttcaggact tttatcggtg ggtatcgaag tgttccagct aggtgtgatc tctacaccag
    34021 gtgttgctta tctgacacgt ttgcaaaaag caagcgcagg ggtcatgatt tctgcttctc
    34081 ataatccagc acaagataat ggaatcaaat ttttcggtgc agatggtttt aaactagtag
    34141 atgaccaaga gctagaaatt gaagcattat tagatgctga agtggatgaa ttgcctcgtc
    34201 catcagctga aggattggga actgtagaag aatttccaga aggcttattg aaatattctc
    34261 aattccttgt acaaacgatc aacggtgacc tttccggttt gaccatttgt gtcgatgcag
    34321 caaacggagc aacttcaaca agtgttaatc gtttgtttgc cgatcttgaa acagattttt
    34381 ataccatggg cacaaatcca gacgggttaa atatcaatga tggcgtaggc tctacccatc
    34441 cagaacgttt agctgagatg gtcgttgaaa aaggagcaga tgctggacta gctttcgatg
    34501 gcgatggcga tcgtattatt gcggtggatg aacttggaaa cattgtcgac ggtgataaaa
    34561 tcatgtttat ctgtgctaaa tacttggcag aaaaaaatcg attgaaaaaa gatacgatcg
    34621 ttacgactgt catgagtaac ttaggattcc acaaagcggt ggaaggaatc ggtttgaaag
    34681 atgtcgttac acaagttggt gatcgttacg ttgtagaaga aatgcgcaaa aacgattata
    34741 actttggcgg cgagcagtct gggcacatga ttttcttaga ttttaacaca actggagatg
    34801 ggatgctttc aggaattcag ttactgaata tcatgaaaca gacaggtaag aaattatcag
    34861 agcttgcagc agaggtaaca atctatccgc aaaaattagt gaatatccga gtaagtgata
    34921 aaaacggtgc gatggatgtt ccggcaatca aaaaagtgat tgaagaggca gaagctgaaa
    34981 tgggatcaga aggaagaatc ctagttcgtc cttccggtac agaaccatta cttcgagtga
    35041 tggcagaagc gcctactgat gagaaagtta attactatgt cgataaaatc gcggcagtag
    35101 tcaaagaaga aatcggactg gattaagaga tattttcata aaaaataaaa gaacaaggac
    35161 cagtgtcaga tgtctgacac tggtccttgt tcttttatta ttctactgtc actgatttcg
    35221 ctaaattacg tggtttgtca acgtcgaatc cccgtaatag cgttgcgtaa tatgcaagta
    35281 attgagtcgg tacgactgtg gttagagtag ttagataagg gtggacagtt ggtagcacca
    35341 gctggtcact ttccttttct aatccctctg agacaataac tagtgtttta gcgccacggc
    35401 tttcgacttc tttcaggttt cctcgagtat gcgcaccagt cacttcttca ctgatgattc
    35461 caatcactgg tgttccttct tcgatcaaag caatcgttcc atgtttcaat tcgccagcgg
    35521 caaaaccttc tgcctggata taactgattt ctttcaattt caaagcagct tcaagagaaa
    35581 cgttgtagtc agcgccacgt ccaatgtaaa aggcatttct tgtatcgctg agatatactt
    35641 ctgccaactc ttctaaataa tctttttggg cgatgaccga ttccatcgca ttagcaacta
    35701 aactcaattc atgagtgata tcgaatacaa gagaagtttc aaattctttt ttatcaccga
    35761 tagctttggc taataaagtt agtacagcta tttgtgcagt ataagctttc gttgatgcta
    35821 ctgcgatttc gggtccagca tgcaatagca gtgtaaagga tgcttcccgc gaaagagtag
    35881 aaccagcaac atttgtaatc gtaagtgaag ggtagccgag acggttgatt tttaccagaa
    35941 cttggcggct atctgctgtt tcgccacttt gactcaagaa aataaagaat ggtttttttg
    36001 aaagaagagg tgtgttatag ccaaattcac tggctaaatg aacctcaaca gggatctgtg
    36061 tcaatttttc cagagtctgt ttacctgcta accccgcatg gtaactagtt ccgcaagcaa
    36121 cgatatagat tcgatcgcta tcagacaatg tatcgaccag cttttgatca agagagacac
    36181 gattgtcttc tgttacgtat ttttgagcga tacgacgcat gataataggc tgttcgtcga
    36241 tttctttcag catataaaaa ggataggtac ctttctcgat atctgaagaa tctacttgtg
    36301 cttcgaatga agaacgttct acgatgtcac ctgcaaaatt ttgaataata actttttctg
    36361 atgtgacggt aaccatctct tcatcttcaa tctcaacgaa ttccttcgta tgagcaagca
    36421 tagccattgc atcgctggca atcacattga atccatcgcc gagtccgatt aatagtggac
    36481 ttttattttt agcgacgtaa ataacatcag gtgctgtccg atcaatcaaa gcgaaagcgt
    36541 aagaaccttt gatgacccgc aaagctttta aaaacgcctc ttgcgttgta gtatccttcg
    36601 cgaaagtttc aattagatga gcgataatct cagtatcggt ctcaccaata aagtggtcat
    36661 ttgctaaata agcaatttta agttcttcaa agttttcgat tacgccattg tgaacgagca
    36721 cgaagcgtcc actttgggaa gtatgtggat gggcgttttc ttcagaaggt tgcccgtgag
    36781 ttgcccagcg tgtatgacca atacctatat ttcctgtagt atcaatattt attttgtttt
    36841 tcaaattttg aattctgcct tgtgctttga ccagataatc agttgtgcca tctgagacaa
    36901 agataccagc tgaatcataa ccacggtatt ctaatttttc tagaccgtca atcaatcctg
    36961 gtgttacatt ttttaatcca atcattccaa caattccgca cataaaaaac atcctacttt
    37021 ctataatgaa ttcgtatgaa tgcttccagc tgcaggaaaa tccttttgtt ttatctttgc
    37081 agatattttt tgtggatttt ctgtagagtg cagtccctag ggccatccgc cgaatctttc
    37141 gataaaccct ctcctcgtca tctgtttaca cagacctggc gctatcctaa aaggtataac
    37201 tatcctcctc ttttgttttt tatctggaaa catttaaaat cataaccttt tgcgaatgtt
    37261 cttgtcaaca aaaaaagaat aaaaaataaa ttggtatata tcaaatgctt tgtcaaatgc
    37321 tctttttatg tcaaaaaata atgggttttt atctatcatc tttttcagga gttcggtaca
    37381 atagaaagta atcggataga ggaggagtaa aaatggattc aaaaattgag atcatgacac
    37441 tgggaatgtt aaaaaaacaa ttgtccaaat tcgaagcatc agctggtgta tcagatgata
    37501 cgaaaatatt tttagatact ggctgggata gcatccaaga aatcgcacct gatgccttgg
    37561 aagttgctga agctcgtgag tttacggtag aagatgaatt gacaaaagag tcgttttccg
    37621 ggtatgcgcg ggaagaaaaa gcagagcggt tcgacacttc tgagcagtca gaaactgtga
    37681 tcgtcataaa aaacttgtat tagaaaggag aaagaatgaa taagagacgt tgggcagcag
    37741 ttctgattgc tgcaggatta tttgtggttt cgttagtttc agctagtttg actaagcctc
    37801 aggaaaacga tcaaattgag ggattgaaca gttggttata tggggatgaa gaactagctt
    37861 caaatgtgtt ggaagaggga gacagtacgc aaaaaatcgt aaaattatca gtagatggaa
    37921 cgattgctgc gggaggggct tcaggtttat ttgcttctga aggttacaat cataaaaatt
    37981 ttatggaaca actgaaggcg attgaagaag atccaacagt caaaggtatc tttttagaag
    38041 taaattctcc tggtggcggt gtctacgaaa gtgcggaaat cgctaagaaa ttggatacga
    38101 ttcgtaaaga acatgatatc ccaatgtatg tcagcatgaa aaacatggca gctagcggtg
    38161 gctactatat ttctgctcaa gcagataaaa tctttgcaac agaagaaaca gtgaccggct
    38221 caattggtgt gatcatgtca ggattgaatt attcaggact tctagaaaaa ctgggtgtgg
    38281 aagatacaac agttaaaagc ggagcgttga aagatatggg ttcaggaacg cgaccagaga
    38341 caaaagaaga agaagcggtg cttcaagcat atatcgacag cgcatatagt cgttttgtag
    38401 caatcgtcag cgagggacgg aataaatctg aagaagaagt caaaaaaatt gctgatggaa
    38461 ggatttatga tggtgtccaa gcaaaagaag taggtttagt cgatgaattg ggatttcctg
    38521 aagatgcttt aaaagctatg cgaaaggaac aacagcttga agatgcggaa ctagtagaat
    38581 attcttccac gccatctggg tttgctagta catggttagg aagtaaacta gcagaattcc
    38641 agggattgaa gccttctgag actagtcaga tcttatccgt tcttgagaat ctagggacag
    38701 cggaatcacc aaaagcaatg tattattacg gaggtgagta agcatgtcag agctttcaaa
    38761 tgaaaataag gaacaagaaa aagataagca acagcagcct gctgtaggac ctgactccaa
    38821 cttttcccaa aaggtactga gctcttttga aagagagcct ttgacgcccg aagaaatcaa
    38881 aaagaaacaa cgtaaatgga aaaagtatac ggacacaaga aatgaaccgc caaaagcaaa
    38941 tgattttcct aagtatttct atgcaggttt ttttatccga atgtttgctt tcttggtcga
    39001 tcttttgtgt atcggagcta ttacgcgttt aacacttggt attgccaaaa atctaggttg
    39061 gcttaatttt tcagattctt atctgagtat ctacggattc acagccttac ttatttatct
    39121 agcgtatttt attttattga cgaaattcaa tcatggtcaa acgattggga aaatgatctt
    39181 tggtattcga gtcatcagct ttgatgaaga agaacttagc tggtcaaccg ttttagttag
    39241 agaaggtgct tgccggttca ttctaaaatt tcctttgctg atgttagggt atcttccagc
    39301 aatctttaat cagaaaaagc aacatctagg cgattttttc actaatacaa gtgttgttac
    39361 gatcaatctg atcaaagcat tcaaccagga agtcaacagc tgatgagcaa taaaaagcag
    39421 taacagttga aagcaccttc taaaagtcag atctaaaatc tagcttttag agggtgcttc
    39481 agttattttt aattgtcatg ctttttttgt tttgctacta ttgaacatga gctgaacatg
    39541 agcaagagga ggacactgcg tgaagccgat tgattttcca gaaaaatatg agaatttaat
    39601 gcgagtagca caacaagcac tagcaaacca gcaatatcag caggcaaaag aattatttca
    39661 acgagcttac gagttgaaag aaagctttga ggctaatagc ctgcttgttt tttgtcttta
    39721 cgagttggac gagaaaaaag aagcattgaa gcaagcactc ttgcatgaaa aacagtatct
    39781 tcaaaatgag gagtttgctg aattttattt tgacttgtta atcagtacac aagatttttt
    39841 atatgctaga aaattgatag caagtacaga tttttatgaa gctttcgagc agcgggtcat
    39901 tgaaaaaatc caatttgcag aagaattatc cggacaaatg gagaggcaga agatcaaggc
    39961 actccacaat aaatcggagg aacttccttc gttggaacct gcacgtcagc ttgcgtctat
    40021 tgatgagatc gaacagcttc cttatcatga gtttatccaa acagcgagta aacttattgt
    40081 atcgccagag gtccatattt tagcaagggc aaaattactg gagacactta ggcaattgaa
    40141 cgaatgccat cctgttttct atttgacgat tgaagaaaag ttagtcaaag tcataccaaa
    40201 agatctgcca aagccgcagc agcaggcaag ttaccagcaa ttatgtgtat tttcagatcg
    40261 ttacggtaat gaagatgcgc tgcttagttc cgtgttaaat gaagaattca cgttgcaatc
    40321 tgcaattgtt tatcctgtct atgatacata catcgaagac ccgaagcgct ggtttcaact
    40381 gactgtagag gcgtgtacag gaaaaacgat gtatgaaggt acagaagatg aaaaacaaga
    40441 ttttttaaaa aaaagagaaa aaattttgca gcaaatgatc ttcttccatt agattttatc
    40501 agtgaaaaag ataaaaatgt tatgaaaaac aaggattcta tccattcaat atgtttacaa
    40561 tttgaaaggg ttagtgtact atttaaaggt atgcatgaaa cgtttttgat gtataaaatg
    40621 aatttctaaa ctcggaggga attaaatgac tgcaacattt gaaaaaaaag gcacgaatga
    40681 tggtgtttta aaatttacga ttgcgcaaga cgaaatccaa aaaggattaa caactgcgtt
    40741 taataaagtg aaaaaaagct tgaacgtacc tggattccgt aaagggaaag tttctcgtca
    40801 agtattcaac cgtatgtacg gtgaagaagc tttatatgaa gatgcattga atgctgtatt
    40861 accagaagct tatgaagctg ctgtcaaaga agctggcatc gatcctgttt ctcaaccaaa
    40921 aatcgatgtt gaaagcatgg aaaaaggtca agactgggta atcactgctg aagtaacagt
    40981 gaaaccagaa gtaaaattag gacaatataa agatctagtt gttgaaaaac aagaccgtga
    41041 agtaacagat gctgacgtgg atgcacgtat caaacgtgaa caagaagcac aagctgaatt
    41101 agttatcaaa gaagatgctg cagctgaaaa tggcgataca gtagtaatcg acttcgaagg
    41161 ctttgtagat ggcgaagcgt ttgatggcgg aaaaggcgaa aattactcac ttgaattagg
    41221 ttcaggctca ttcattccag gttttgaaga tcaattagtt ggtcataaat caggtgaaag
    41281 tctagatgta aatgtaactt tcccagaaga ttatcaagca gaagatcttg ctggaaaaga
    41341 agcagtcttc aaagtgacaa tccatgaagt aaaagcaaaa gaagtgcctg aattagatga
    41401 cgaattcgca aaagatgttg atgacgaagt atcaacatta gacgaattga aagaaaaata
    41461 tcgcaaagaa ttagtagaaa caaaagaaaa agcagcggac gatgcaaaag atgaagcagc
    41521 gatccgtatg gctgttgaaa acgcagaaat cgttgaattg ccacacgtga tggttcatga
    41581 tgaagtacat cgttcaatgg acgaattctt gaacaacatg caacgtcaag gtatctctcc
    41641 tgaaatgtac tatcaattaa ctggttctac agaagaagac ttgcataaac aattcgaagg
    41701 tgaagcagaa atgcgcacaa gaacaaatct tgtgatcgaa gcaattgcag ctgctgaaaa
    41761 tcttcaagca acagaagaag aaatcaatgc agaaatccaa gaattatcag aaacatacaa
    41821 catgccaatc gaacaaatca aacgcgtgtt gacagaagat atgctggaac atgatgtgac
    41881 aatgaaaaaa gcaatcgaat tgatcacagg tacagcgaca gaagcgaaag aatagaaatc
    41941 aagtaataaa tcatttctga agaagctact tctagaaaat tgtttattcg aaataaggga
    42001 ctgtgacacg attttgtcac agttccttat tttttgttaa gagtaaaaaa tgcaaaggta
    42061 taggtcagtt gatcctttgt taaacacttt ttctttttac taaactatgt tatgatagta
    42121 aaagactgta aagtctttga tagtttcttg catacttttt gcaagttaag gggtgaagac
    42181 catgtatgac aatccaagta gcaatgagac cgtacgctgt tctttttgcg ggaaaaccca
    42241 agaagaagtg aaaaagatcg tagctggtcc aggggtctac atctgtaatg aatgtatcga
    42301 cctatgtaaa gaaatcattg atgaagaatt ttatgaagaa gctgtacgcg aattcactga
    42361 agtccctaaa ccactagaaa ttcttgaagt attgaataac tacgtgattg gacaagatcg
    42421 cgcaaaacgc tcgttagctg ttgccgtata caatcactat aaacgtgtca atcagcaagc
    42481 acaagaaaac acagatgatg ttgaactgca aaaaagcaat atctgcttga tcggacctac
    42541 agggtctggt aaaacatttt tagcacaaac attagcaaaa acattgaatg taccttttgc
    42601 aatcgcagat gcgacaagtt tgacagaagc tggttatgtg ggggaagatg tagaaaacat
    42661 tttgcttaaa ttgcttcaat ctgctgatta caatgtggaa cgtgcagaaa aagggatcat
    42721 ctatattgat gagatcgata aaattgcgcg taaaagcgaa aatgtatcta ttactcgtga
    42781 cgtttccggc gaaggtgtcc aacaagcctt gctgaaaatt ctagaaggaa ctgttgcaag
    42841 cgtgccacct cagggaggac gtaaacatcc acatcaagaa tttattcaga ttgatacgac
    42901 gaacatattg ttcatcgtag gtggtgcatt tgatggcatc gaaacgattg tgaagaatcg
    42961 tatgggcgaa aagacgattg gatttggtac acaaaaccaa aaactttctg aagacgaaag
    43021 tgtcatgcag catatcattc ctgaagattt actaaaattc ggattgatcc ctgagtttat
    43081 cggacgatta cctgttatgg cagctttaga gaaattgaca acagatgatc tggtacggat
    43141 cttgactgag ccgaaaaatg cgctagtcaa acaatatcaa aaacttcttt ctctagataa
    43201 taccgaactt gaattcgaac ctgaagcgtt gagagccatt gcgaaaaaag caatcgaacg
    43261 caataccggt gctcgaggac ttcgttccat cattgaagaa atcatgatgg atgtgatgtt
    43321 tgatatccct tcaaatgaaa acatcgaaaa agtgattatc acaaaagaag cagctgaatt
    43381 gtctggtgag ccaacagtaa tctataaaga ttcagagaaa aaagcgggtt gatgaaaaga
    43441 ggctgccgta aaagtcattt tggcttttgc gacagccgct tgctttattc atcaccttgt
    43501 taaggagaga gaccatgaaa gtccatcaag ctgaaattat cataagtgca gtggcaccaa
    43561 aacaatatcc agatacacaa ttgccagaaa tagctctggc aggacgatca aatgtaggga
    43621 aatcttcttt tatcaacaca ttgattgatc gaaagaattt agcccgtact tccggtaagc
    43681 caggaaagac ccagacattg aatttttatt tgatcgagaa cgcattacat tttgtcgatg
    43741 ttccgggata tgggtatgca aaagtatcca aaactgagcg agcaaaatgg ggaaagatga
    43801 tcgagacata tttaacgact agagaccagc tacgtgcggt cgtttcttta gtagatttgc
    43861 gtcataagcc ttctcaagaa gatatccaga tgtatcaatt tttgaagtat tatgatatcc
    43921 ctgtaattgt tgttgccact aaggcagata agatccctcg tggtaaatgg aacaagcatg
    43981 agagcgaaat caaaaaagcg ttggattttg atcgcaatga cacgtttatt ctcttttctt
    44041 ccgttacaaa acagggaaaa gaagaagcgt gggcagcaat cgaacaagca attgaatcat
    44101 aaaaagaata aaaaagactt accagtcaaa ctctttcata aaagtttggc tggtaagtct
    44161 ttttttgagc ttaacttact tttgtttctt gtcttttttc ttttcatttt gtggctgtgg
    44221 ttttagaaat cgatcgatct cttcttttgg tgcaactgtt ttttccggat cgacagcaaa
    44281 tgattcgaaa agtttatcta aattatctgt acgttcaaat ttcaacccca ttgtaaacac
    44341 ctctttgttt ctttttactt agtctatcat gtaatcgtcc aaattggttt ctaagacata
    44401 aagttttagt ttttctttat cgttggttat aattaaatta taaaaagatt ttaaagaggt
    44461 gagaaaatga ttggcgaaga taaattattg atgactatcc agctcacaga cttaaatgaa
    44521 gaagaaaaaa aacaggaaat aagcgattgg gctattttga cacgtctgtt gatccagcca
    44581 acatttatcc gctctttcac acaacaagcg gatccgtatg atcttcgaaa gcttcaagaa
    44641 aaaataatgc tagcatcggt aagagacgaa agttggttag ttgtaggaaa tgacgagaac
    44701 gaatgtagtt ttcgcttgga agataatcaa ttattgataa aaaatgtatt gtctatctct
    44761 gttttcaaag aaaagcaatt tttgattcga gacttcattc agaaaaaaat gattgctcat
    44821 ggggtgttcg cctatatgcg agcttatagc gagtttatct atcacaatac aaaacagatc
    44881 tctcaacggc tattgtttga gaaaaaggat gaaatcgaac atttacctaa aatgaaacaa
    44941 caaaacgggg aagtagtagt cgattgcaac caatttccag gatacgatct tttttaccaa
    45001 ggtttatgtt tcacttcgtg ttgggaaatg tattatagtc gctattatca tcaaatcatc
    45061 cctaaaccga tttttctcga catccaacaa gtagaaaaag tgaaggaatt agaaaacgaa
    45121 gtcatatgta tccagcttta tcgagacccc tttaactggc aaaagccaaa caatcaaatg
    45181 tttcaatcct attttaggga ccagcttggg tttgatcatc ttgcttggga caatggtgtc
    45241 ggtttattga aaccgccatt cgttgaatac atttacactg accatacgat ccaatctgtc
    45301 cagtatcaga atgcacaaat gcaacctgtt cctaaaaaga atgcttcatt ttttgtaaca
    45361 aaaagttatg atattcaaca aggagattat aaagaaaggc gtgttcgcgg aacactaaat
    45421 gctcaggcgt attttccttg ggttgacgag aatcgctcgc gcatgatgtg ctataaagtt
    45481 atcgatccga ctgttgcttt ggataatgga atcgaagctt attgttatta tattagggaa
    45541 tatctagaag tagaggtaac agatgaaaaa tatcaagagt atcttttatc cttaagaatc
    45601 tatgttcctt cagaatccct gtcagaatta ccgttaaagg aaatcaaaca tagactctct
    45661 gatgttacct tcaaacgaat aaagaaaaag cggcgaagta tccaattcga tgtcaagaaa
    45721 ggtgagcagc atctgcgtgt gcaattttta gattaccgag aattagaaca attgattact
    45781 ttacaaaaaa tatgaggtga agaaaatgga taaacaactc tattatcgaa taatggttcc
    45841 ggcatcatta ttatccgtct gtctcttttt tgttcctaat tttcctattc gatcagtcat
    45901 cggttttttg gtcatgtttg gcgggatggg actttattat tatcttcttc ataaggaaag
    45961 aaaacctgct tctgtttata tgaaaaaaac aagaag
//
LOCUS       DAZULG010000012        42796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000012 DAZULG010000000
VERSION     DAZULG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.012"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..3351
                     /locus_tag="ACG9CY_000563"
     CDS             73..3351
                     /locus_tag="ACG9CY_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS4937100.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3892..5799)
                     /locus_tag="ACG9CY_000564"
     CDS             complement(3892..5799)
                     /locus_tag="ACG9CY_000564"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS4937101.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMENAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5929..6804
                     /locus_tag="ACG9CY_000565"
     CDS             5929..6804
                     /locus_tag="ACG9CY_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS4937102.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6884..7858
                     /locus_tag="ACG9CY_000566"
     CDS             6884..7858
                     /locus_tag="ACG9CY_000566"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4937103.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8038..9183
                     /locus_tag="ACG9CY_000567"
     CDS             8038..9183
                     /locus_tag="ACG9CY_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4937104.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9320..9664
                     /locus_tag="ACG9CY_000568"
     CDS             9320..9664
                     /locus_tag="ACG9CY_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS4937105.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9935..11221
                     /gene="efmA"
                     /locus_tag="ACG9CY_000569"
     CDS             9935..11221
                     /gene="efmA"
                     /locus_tag="ACG9CY_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS4937106.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11359..11958)
                     /locus_tag="ACG9CY_000570"
     CDS             complement(11359..11958)
                     /locus_tag="ACG9CY_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4937107.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12362..13483
                     /gene="mnmA"
                     /locus_tag="ACG9CY_000571"
     CDS             12362..13483
                     /gene="mnmA"
                     /locus_tag="ACG9CY_000571"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS4937108.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13729..15150
                     /gene="arcD"
                     /locus_tag="ACG9CY_000572"
     CDS             13729..15150
                     /gene="arcD"
                     /locus_tag="ACG9CY_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS4937109.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15164..16282
                     /locus_tag="ACG9CY_000573"
     CDS             15164..16282
                     /locus_tag="ACG9CY_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS4937110.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16342..17634)
                     /locus_tag="ACG9CY_000574"
     CDS             complement(16342..17634)
                     /locus_tag="ACG9CY_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4937111.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17784..18344
                     /locus_tag="ACG9CY_000575"
     CDS             17784..18344
                     /locus_tag="ACG9CY_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4937112.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18473..18850
                     /locus_tag="ACG9CY_000576"
     CDS             18473..18850
                     /locus_tag="ACG9CY_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS4937113.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18783..20855
                     /locus_tag="ACG9CY_000577"
     CDS             18783..20855
                     /locus_tag="ACG9CY_000577"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4937114.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21066..22013)
                     /locus_tag="ACG9CY_000578"
     CDS             complement(21066..22013)
                     /locus_tag="ACG9CY_000578"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4937115.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22259..22723
                     /locus_tag="ACG9CY_000579"
     CDS             22259..22723
                     /locus_tag="ACG9CY_000579"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS4937116.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22785..23828)
                     /locus_tag="ACG9CY_000580"
     CDS             complement(22785..23828)
                     /locus_tag="ACG9CY_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS4937117.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24205..25170)
                     /locus_tag="ACG9CY_000581"
     CDS             complement(24205..25170)
                     /locus_tag="ACG9CY_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS4937118.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25404..26249
                     /locus_tag="ACG9CY_000582"
     CDS             25404..26249
                     /locus_tag="ACG9CY_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4937119.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26440..27627
                     /locus_tag="ACG9CY_000583"
     CDS             26440..27627
                     /locus_tag="ACG9CY_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4937120.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27633..27989
                     /locus_tag="ACG9CY_000584"
     CDS             27633..27989
                     /locus_tag="ACG9CY_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS4937121.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27991..28329
                     /locus_tag="ACG9CY_000585"
     CDS             27991..28329
                     /locus_tag="ACG9CY_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS4937122.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28405..28661
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28724..29758
                     /locus_tag="ACG9CY_000586"
     CDS             28724..29758
                     /locus_tag="ACG9CY_000586"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4937123.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29751..30437
                     /locus_tag="ACG9CY_000587"
     CDS             29751..30437
                     /locus_tag="ACG9CY_000587"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4937124.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30461..31309
                     /locus_tag="ACG9CY_000588"
     CDS             30461..31309
                     /locus_tag="ACG9CY_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4937125.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31506..32279
                     /gene="sufC"
                     /locus_tag="ACG9CY_000589"
     CDS             31506..32279
                     /gene="sufC"
                     /locus_tag="ACG9CY_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS4937126.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32292..33578
                     /gene="sufD"
                     /locus_tag="ACG9CY_000590"
     CDS             32292..33578
                     /gene="sufD"
                     /locus_tag="ACG9CY_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS4937127.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33575..34810
                     /locus_tag="ACG9CY_000591"
     CDS             33575..34810
                     /locus_tag="ACG9CY_000591"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS4937128.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34797..35267
                     /gene="sufU"
                     /locus_tag="ACG9CY_000592"
     CDS             34797..35267
                     /gene="sufU"
                     /locus_tag="ACG9CY_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS4937129.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35272..36663
                     /gene="sufB"
                     /locus_tag="ACG9CY_000593"
     CDS             35272..36663
                     /gene="sufB"
                     /locus_tag="ACG9CY_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS4937130.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36758..37900)
                     /locus_tag="ACG9CY_000594"
     CDS             complement(36758..37900)
                     /locus_tag="ACG9CY_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4937131.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(37939..38673)
                     /locus_tag="ACG9CY_000595"
     CDS             complement(37939..38673)
                     /locus_tag="ACG9CY_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4937132.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            38905..39180
                     /locus_tag="ACG9CY_000596"
     CDS             38905..39180
                     /locus_tag="ACG9CY_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4937133.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            39278..41275
                     /locus_tag="ACG9CY_000597"
     CDS             39278..41275
                     /locus_tag="ACG9CY_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937134.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(41351..41686)
                     /locus_tag="ACG9CY_000598"
     CDS             complement(41351..41686)
                     /locus_tag="ACG9CY_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4937135.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            41814..42028
                     /locus_tag="ACG9CY_000599"
                     /pseudo
     CDS             41814..42028
                     /locus_tag="ACG9CY_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            42065..42427
                     /locus_tag="ACG9CY_000600"
     CDS             42065..42427
                     /locus_tag="ACG9CY_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS4937136.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            42473..>42796
                     /locus_tag="ACG9CY_000601"
     CDS             42473..>42796
                     /locus_tag="ACG9CY_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937137.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        14018 a         7660 c         8989 g        12129 t
ORIGIN      
        1 ttattgacag taaaaataaa ttaggtaata ctttaagtaa acgctttcta atttgttacg
       61 ggaggaatac acttgttaaa aaaacgtata aaatacagtg cgctaatgat gaatctcctt
      121 atgctcagta ccccggtttt aatttctgtc gttgaagttg ctgctaacga acaacagtct
      181 gtgaacgaag aaaatgcaca cgtgaacatt gaagggatgt caacaatgga ggaaatacca
      241 agaactagtg aagatatgga tacggaagaa ggaaatgatc ctatgatgaa agaatcagaa
      301 aatgaaaaga caaaaaagca atctgaagaa gcaaatgaag agtggaaagg ggttgttttc
      361 ggagaaagca caagtgctag taggtatgcc attgaaccat tagaagacgg cgtgcggctc
      421 tcttccacga ataacggcgg aaaattccag tcaagcggat cagatggaat gacctattat
      481 tatcaagcaa tccccaaaga catcaacttt aggatgcggg cgacgatcga aatagaatct
      541 tggacctata cgaatgccca agaaggattt gctttaatgg taagagacgc agtaccaaaa
      601 aatcatttgt ttgggcaggc attttattca aattcatttg ccatactagg cagtcggatc
      661 gaatatgtct gggactcgga tagacaagaa gtggtcaaca cgagcggcag caaatatacg
      721 atgcgacttg gattggggac acgggcaatc acaggaatat catctgaaga tccgcaagct
      781 gcgccagctc ctgggagtgt ctcagtggaa acaacacctt tagagacttc agcagggttc
      841 ttggaaaaag aaacaggttc ttataatata ttcggaaacg gtcatgggaa tctgtttccg
      901 gccacgaact cgattactaa actggacgta gagatgaaaa aaacgaatac tggtttagag
      961 gcatattatt acgatcctga aacaggagaa gaaatggctt ctgacatcat gtatgactgg
     1021 gaaaaattat acgcttcaga tgaatcggtt atttatgcag gatttgctgc agcaaggaat
     1081 atgacgatca aggtagaagg aatcgaagtt gcttactcag atcctgcgac agatcctcct
     1141 gggcaagaaa gacctactcg cttgattgat ccgatttaca ctgtgacctc tagtaatcat
     1201 tcaggaaacc aagatcatac tatgtccttt agagcaaatg ctgacggatt attgacaatt
     1261 aaaaataaag caacaggaga ggtgcttaag aacgcaaaaa acttggctat cactgcgaat
     1321 cgggaatttg attatcagac aaaactgatg gaaggaacga acgaatttac tttcagtttt
     1381 acgccagata aaaactatcc gccagaagaa tacgtagaga tgactagtta tgaaacaaaa
     1441 gagattcttc atacagtgga ctaccgtaaa cctaaatatt cgacagttta tgtaacacca
     1501 gaagggtcag atgcaggaaa cggaagcaga caaaatccat tatctctgac acaagcattg
     1561 agatacgcat atgctgggca gacaatcgtc atggcgcctg gaacttattc cttcgaaaga
     1621 ggattgacga ttccaaaagg cgttaacggg acaagagaaa acccgattca gttgattgca
     1681 gagaaaaatc cagaaaacaa gcgtcctgta cttgatttta aaggaactgg aaatgggatg
     1741 acactgttca gtcactactg gggtttacga gggtttgaca ttacaaatag tgcagctatg
     1801 caaaaaggat tacagatttc tggaaatcat aatttgatcg aagagattca tgcttatcgg
     1861 aatggaaata caggaatcca aatcagcggt agctcaaatg atcctttcga agcatggccc
     1921 gcgcataact tagtcaagaa ctgtacatct tttgaaaatg ccgatccagg atttgaagat
     1981 gccgatggat ttgcggccaa actgacaata ggagaaggaa atgtatttga cggttgtatc
     2041 gcgtatcata atgcagatga cggttgggac ctttttgcta aaaatgaaac aggttcgatc
     2101 ggaaaagtaa tcatcaaaaa ctcagttgct tatcgaaacg gctgggtacc aggaattgag
     2161 ggagaaggta acggtaatgg attcaaaatg ggcggttctt ctttaacggg ccctcatgaa
     2221 ttgatcaata gtttgtcttt cgaaaatctt gcgaaaggaa tcgattccaa tagcggaaca
     2281 gatattatcg tcaatagcag tacaagtttt aataacggtt catataatgt ggctctttat
     2341 acctcttcag caggaaacac agactaccat gcttctggta tcttatcttt ccgaacagag
     2401 aatcttgaga tgagagagca gatccgccca ttgaatcaaa acgaagatca aatctaccac
     2461 tcgttgaact actattggaa tgaacgagag aaacaatcag aaaatactct tggacatacc
     2521 atatcaaaag attggatcga gtctctaaat acatgttcga aagaaggtca gcagcctgtt
     2581 acgcgctatg aaaatggaag tatccatgtt catggactga tgcagattaa accggggaat
     2641 cgtcaaacag aaaaggaaag agtggatggt cttccattat ctgtaggtgc tatttttgac
     2701 ggagaaacct caccgtcttc ggaatatcca gagtatgcaa tcgtacaaga accaaatcag
     2761 acaaaaccaa atgaacaaaa taatccgatt attatacaag aagcaaatgg tgtggcggag
     2821 aatgcagaag gaaatatcat tgaactgaag ccttttacag tttttgtgtc aactttcaca
     2881 ccaaatcaag gaacgcctgt ctttgaagat atgcgttatt tcccagaaaa gatccaaatc
     2941 gttgaaacac agtcaagtgc tcaattttct aatcataccg ctattttttc agcaccttca
     3001 aaaggagaat atacgctaga agtcacttat cgttcggaaa tatttgatgg ggaaaaatgg
     3061 tctgctcata cagaatctgt tacccagcaa aaagcgatca ctgtgcgaga aactgccgat
     3121 gtgcccccgg gaaatgacaa accagaaaga gaaaatccag gaaatgaatc accaaatgga
     3181 aaaccaaatg gaaaaccttc tcatccggat aggacagcaa aagaaagtga aaaaaatggc
     3241 actcaaggac tcccaaaaac gggagaattt aataacccgt tgctagcatt agctggagga
     3301 attttgttaa tcggtgtagt tgtttatgtc atgaaacagc gaaaaaaata agtggaattt
     3361 ctccttttat gatgtaaaac aattgactga ggaaagtaaa aatagaagga aatgagacca
     3421 atagatgtct cacttccttc tatcttttta tagatgaggt tctccttgct gcttttgatt
     3481 ggctgttctt attacttgat gcaggacagc tttttcacaa tctctccact aaacggtgtt
     3541 caatcagctg accctcggta ttagctcaac aaacggcaaa atatgaggaa ctatttttcc
     3601 gtttttttct ctaatactca ggtcaggacg ctgatttcac aacctctcga taaacggtgt
     3661 tcaaaagcca gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa agccattttc
     3721 acaaaatttt tcttactact tgaaactaag cagctttttc acaacctcta atacatggta
     3781 ttccagaagt aacttcttcg aaaaataagc cgaaatgtca caaaaatttg agaagcgatt
     3841 ttcccaaatt ccttcttatt tcttgaagct gaccacttct ggcacaacct cttaccagat
     3901 tcgcactctt ttttctggcg ctagatacat agcgtcttgt tccgtgatac caaatgtttc
     3961 atagaattcg tcaaggtttt ttggttgcac gtttgctcgg agtttagcag gggcatggac
     4021 atcgatttgc agtaaaagtt gttggtattc tttttttgct tttgtacgcc aaatcgttgc
     4081 ccaattgaca aagaattcct cgatagaaag atctgattcc gtttttgctg cttccaatgc
     4141 acagcttaga ccgcctgcat cagcaatatt ttccgaaacg gttaattttc cgtttacttt
     4201 tccgtcagca aaaggcagac catcgaattc ttcgatcatt gcttgtgctt tgttttgaaa
     4261 ttgtgtaaga tcttcttctg tccaccagtt gttcaagttg ccatattcgt caaataaagc
     4321 gccgttatta tcaaatgcat gggaaatctc atgagcgata acagctccga ttccaccata
     4381 attagcagag cttgattgtt ctagactgta aaatggtgct tgaaggatcg ctgccggaaa
     4441 gacgatcaca ttccgaaatg gatgataata agcattgacc gtgtcagcac tcatttccca
     4501 ttctgaacga tccactggtt tgttccattt gctgaaacga tcttttaatg tgagtttgct
     4561 gaaaacaaga gtatttgaaa gcaaagtacc accctgatct gcaggtactg ttttgaactg
     4621 gtcgaataat gctgggatct tatcggggta gcctacttgg atacctaagt tatttaattt
     4681 tatgacggct ttttcttttg ttgcatcccc aagccagtca ttttcttcta gacgtttttt
     4741 ataaacggcg atcatttttt tcaccatttg ttctacgtct tttttagcag cttcgccaaa
     4801 atattttttt ccgtagtaat cgccaactac ttgatcgaat tgtccagagg caaggtaata
     4861 cgctgctttt ttctgaggca ttgcttcgtc tgttccagac aatacgcgag aatagattcc
     4921 gctgacttgg cggaattctt ctgatagata accactaaga gaacgtactg ttttaatgat
     4981 catccagctt ttgaataacg gaaaatgagc ttctgtcagg atgtcattca aatgatcgaa
     5041 gtacactgga tcagttacga tcactttatc tggagttgtc ccaatcaaac ccgtgacaag
     5101 ttttttcaag tccagtttat ctgtgtattt aataaatgta tcaaaagatt gcggattata
     5161 cattttgctg taatctgcgt tttcttctgc actttttaca tgaggagcaa tcaatttatc
     5221 aaattgcact gcttgttccg tgattgcttc tgcctgactt ttttctttgc cggcaagctc
     5281 taataactga acagccatat caaagaaaac gcctattagt tgtgcaccgt tgggatggtc
     5341 tgatgcataa tacgtcttat ctggaaggaa taaagaaggc ggataagcaa agaaggcgtt
     5401 cgtttgcgca ttctccatat ctgcatctac atccagatta aatggcaatg gtaatgtatc
     5461 caacagccat tctggaaatt gcttattcaa ttcgtcgaaa gactgcaatg attcaacttt
     5521 ttcgattata ggttgaagcg gttgagcatg caactcattt cttcggtcat aatcgtttgc
     5581 cagttgataa tatgcaagga aatgtttcat tttttctgaa gacacttttt ctggatgggc
     5641 aagcagatcg tctgtttctc ccatcaaaag ttggtcgatc ccgtcaacta aatcttggaa
     5701 accgcctgta gctggtttat ctgcagggat ttttgctgtt ttttgccatt cttcattgac
     5761 tgcttcatag aaatcttgtt tcaatcgttc tttgttcatt catcgatcgc tccttttttg
     5821 tttcgtgtgc ccattatctt acaaaatgaa ggaattaaca aatagaacat aaaaaattga
     5881 aaattcgaac aatcaaactg atttgttttt ttcaatcctt cacttataat gaaaaaaagg
     5941 aggcgatccc aactgaatca agtagtggat aaacctttat atttcaaagt agataaaaaa
     6001 attaaaaaat tagccagcac tcagcagctg cagtcaagaa aaagtaaatt gcttttttta
     6061 gctctggttt ttgaagatca aagttatgtg attatcgatc aatcaggaca tccggtagaa
     6121 tattctccag caaaatatac ttatcaagag ggtctttctt gtaaaaaatg gaagctgatc
     6181 aatgaagagc caatcgagtt atctagatgg atcaacagaa aagaagatat tcccgttttg
     6241 atcgaagaaa aaaggtcagg aaaggaactc gcaaattgct gggtgggatt gccagaagaa
     6301 cgttttttga ggtataagaa atgggctacc ccctcaggct atctttgtgg tacttacgcg
     6361 gcagcagttc tccttgccta ttatcaagac tatcgtaagg ggtggatgct tccaaatgag
     6421 attcgcaaga aaaatacggc tgattcgttg gttttgacga aggcattgcg tagccagatc
     6481 caaccactag gacttcctac gattcctttt caggttagta cagggatttc tagtttttta
     6541 aagaagaatg gcaatcatga gcgtgcacga gcaactcttc taggttcatg gcagcgagct
     6601 acaaaacgta tacgtgaagg caaacctgtg atgattggta ttttgaaagt acttggcagt
     6661 acatatggca atcattgggt gacagcgtac gcttattttg aaactgaaac aggagaacgt
     6721 tattacaaag tacacgacaa ctggggcgat tatcataaag tcattcctgc cagttggagt
     6781 aacggaacag tttcacttcc ttaagaattt gctgaaatta acagaaaatc ttggaaaaat
     6841 atgttaaact atcgaagaat agagaagttt tggggaggac tacatgagta ataagtatca
     6901 ggatgacacg ttgaagattt ttagtttgaa tggcaaccgt ccattagctg aaaaaatcgc
     6961 gaaagtgttc ggaacagaat taggtaaaag cgtcgtgaag caatttagtg atggcgagat
     7021 ttccatcaat atcgaagaaa gtattcgagg ggatcatgtg tatatcgttc aatcaacgaa
     7081 tgcacctgtc aatgattact atatggaact actgatcatg atcgatgcaa tgaagcgagc
     7141 aagtgcgaaa acgatcaatg tcgtattgcc ttactatggg tatgctaggc aagaccgcac
     7201 ggcgaaacct catgaaccga tcactgcgaa attgatcgct aacttgatcg aagaagcagg
     7261 ggcaactcga gtactgactt tagatctgca cacggttcaa gtacaaggat tctttgatat
     7321 tcctgtagat aatttattta cgatgccgtt gtttgctcat tattaccgtc aattaggtct
     7381 gacgggagat gacatcgtag ttgtttcgcc aaaaaacagc ggtgttcagc gtgcacgtag
     7441 tctttcagaa tatttgaaca gcacattagc catcgtagat catgcagatg atgcttcaac
     7501 tgagggtggt tatgtgatcg gtgatgttca aggaaagaca tgtatcatgg tagatgatat
     7561 cttgaacaca ggagcaacgc ttgcacgagc agcaaatgta ttaaaagaaa acggcgcaaa
     7621 agaagtatat gcctgtgcgt cacatggatt attgtccgat catgcgaaaa gtattttaga
     7681 caatgcacca atcaaagata tttgtatcac tgattctgtt ttcacagaag aaaatcgtca
     7741 tccagaaaac ttaacaatcg ttacatgttc agaattaatg ggtgaagcgg tcaaacgcat
     7801 ccatgaaaat acaccgatga gtccgttatt ccgtctggaa gaaaaagaat tcgaataaac
     7861 aaaaaaaaga gggaaagaag ttgtgacaga agtgagcagc ttcaaggagc tgcgacaaga
     7921 cttttgtcac agctcctttt ttctatgccc atgaaacgtc ctctagacaa aaatgagctc
     7981 ttctttggtt tgttcgctat tttatgctag aatgtgccta gcaataattt ggaagggatg
     8041 aatgaagtga aaactattta tctggatcat gcggcaacta cccccatgca tccacaagta
     8101 attgaagcga tgacggcaat catgcaagac acgtttggaa atccttcaag catacatgga
     8161 tttggtcgcc acgcgcatga aaaattggaa acagcaagac aaatcattgc agacagtctg
     8221 caagcaaaac ctcacgagat catctttaac agtgggggaa cagaagggga taatacagct
     8281 atcttagaaa cagcgttctc gcgtcaaaaa gaaggcagac atctaatcac tacagctatc
     8341 gaacatcctg ctgttcttaa taccatgaaa tatttagaga caaaggggtt tgacgttaca
     8401 tatctgcctg tagatgaaaa agggaatctg tcgatcgaag aggtcaaaaa agcgttacga
     8461 gaagatacga tcttggtctc tgtgatgtac gggaacaatg aaatcggaaa tttgatgcca
     8521 atcaaagaaa tcggtgaact tttagcggat catccagctt atttccatac agatgcggta
     8581 caagcgtacg gaaaccaaac gatccatcca gaagaattag gaatcgattt attaagtatc
     8641 tctgctcata aaatcaatgg gccaaaaggc gtcggtttct tatataaacg agacggagta
     8701 aatatccctg cattgctttt aggcggggag caggaagaaa agcggcgtgc aggtacagaa
     8761 aatttagcag ggatctgggg tatggcaaaa gcagttgaac tgctgtcacc tgaagaacgt
     8821 gaagcacgta acaacaaata taaaggattc cagcaattga ttcttgatac attagaagca
     8881 aacgaaatcc cttatcaagt aaatggtgat ttaacgaata aactccctca tgtgttgaat
     8941 cttcgcttaa ttggcgaagc aaatgatttg ctactgatga agcttgatct taatggggtc
     9001 gctatttcta ctggttcagc ttgtacagca ggaaatatcg agccttcaca cgtattagaa
     9061 gcaatgtatg gagaagatac accgatacta aaagaatcca tccgtatcag ctttggtttg
     9121 ggaaataccg aagacgacat ccagtatttc cttgatcaat tagttcgtgc tgtcaaaaaa
     9181 taactagtga agtcattctt atatgaacag atgtctgaga gaacgccttc attttttagg
     9241 gaacttaact gaaaaaccta gtaaaaatca ggataaactt ttataataaa gagaaactaa
     9301 ttttatcgag gtgaaaatca tggcatttga aacaacagca acggtactgg gcgcaccaat
     9361 tagctatcgt ttgcatcctg atgcaaaaag atatacattg cgcgataatg gatttacaga
     9421 aacaaacgga ggcaattatc aattgatccg accattagat gccacgccgc aaagcaaaga
     9481 aggattcaaa ttaaaaatca ctgtatcaaa agatattcaa acattgaaaa tgtcgatcac
     9541 tacagcaaat ggattgaaag cagtcaatat tttcaaagat ccaaaacaga aaatgagcca
     9601 agataaattt tattttctga tggatgggat gattagccgt gggttattcg aaaaagtcga
     9661 atgatctgtg gatataaaaa gacaactagt tttgaaacgg cctattgtta gatgactaac
     9721 tttgtaagga cgacgcaaat tagttgtctt ttttattttt aaatttaaca catgttgtta
     9781 tttttaatat tttgctgatt tattgtttct aagtaaagaa atttaaattg aaacgattgt
     9841 tatttaggga aaaaggtatt ttttgaaaaa agaatcccgt ataatgattg ttgtgcaaac
     9901 gctatacaaa atgaaaaaga aaagagggat gtttatggaa aatgaacagt cggtcgtttt
     9961 aacgaattgg aagagaaatt atttgttctt tttatcggga cagtttttgt caggaatcac
    10021 tagtatggtc gtccaatatg cgatcatctg gtatttgaca agagaaaccg gttcggcgac
    10081 aattttgagt tttgctactt tactaggaat gattcccatg gttttactaa gcccatttgt
    10141 aggaccgcta gtggatcgct gggataaaaa agctcttttg attgtcacgg atattattgt
    10201 agcgattttc gcgcttattt tagcagtagt cggaacgatt tcagaatcct tcccgatttg
    10261 gctagttttt gtgtctttgt ttatgcgttc ggtcgcacaa acattccaaa tgccaacgat
    10321 ccagtcgatt atgccgacaa tcgttccatc ttctcacata acaaggacga atggacagtt
    10381 aggaatggtc caatcagcga atttcatcat tgcgccagct ctaggtgcgg ctttgttttc
    10441 agtcgttcct gtcaattacc ttattttatt agatgtattg ggggcagttt ttggtgtcgg
    10501 cttattgatt tttgtgaaga tcccaaaagt ttctcctgaa atattggaag ttccgcttac
    10561 tatttttaaa gatgcaaaat ttgggctgca gcaattgatg gataataaag ggttatggta
    10621 cataacgatc aatggtgcat ttgtgatgct gttgttcatg ccagcaatca gtctgtatcc
    10681 attgatgacg ctagattact ttggtggttc cgttggacag gcgggagcag tggaagtagt
    10741 ctacgcagtc ggaatgctct taggcggtgc actgatcagt tttataggaa catggaaaga
    10801 ccgaatgaaa cctattatta tagcctacat cattatgggc ctgacgatcg gtgcaagcgg
    10861 attggttcca aacgatagtc aagggttctt gtactttctt atcttgaatg caggagcagg
    10921 ttgtgcaacg ccatatttca atacactgct gatggcaatg atccagcaga gttatgaatc
    10981 gaatgtttta ggtcgtgtct tagggaactt caactctttg atgaacctgg caggtccaat
    11041 tggattatta tttgcaggac cattagctga tcgtttaggg gtagaaaaaa tgttcttgtt
    11101 ttccggaatc gggattttat tatgcggaat cgttttattt ttaacgtcag ctgcccgaaa
    11161 ttatgataaa gaattacaga aaaaactggt aaaagaacac catgagcaaa aagacgaata
    11221 gtatggaatg gagttgtgac agaagtcttg ttacaactcc atttctgatc ctaatgttcc
    11281 tgtggcaata agcctactca aatgaaaaat atgagtaatt atctttattt gtttaatact
    11341 tgatataggg cagttttttc acgacctatc gtacttcctc tttgcttcat agatttcttt
    11401 gatttgttgc tcagataacg gagtgccaaa tggtagtttt tcctttaaga gttctctttc
    11461 cggtgcgtct acgcctacat tgacatcgtt ctcgataggt acgctgtaat gatggatatg
    11521 tccatgaaga ttgcggatag tgtcgttagg cccgagcaac atcggataat gagtcaagta
    11581 atattgttga tggtcgaatt taacgatggc acctacatca taaaaataaa atttgggact
    11641 tccgtcagta agcctaggat catttttttc taaataacgg aaaaaagcgc gactgtcatg
    11701 gttccctttg atcaagtgga ttgttccttg cagttgagtg accagttcca aaatttctgc
    11761 atttgctttt tcatattgcg gatgcatagc aaggtcgcct agatgataaa catggtcggt
    11821 ttcttttatt actgcattcc agctagtgat caactgttgg ttcatttcat ccacatttgc
    11881 aaagagccgc ggagcaaagt cgttttttcc taataagcgt tcatggaaaa agtgcatatc
    11941 actgataaag taattcatgt atcttttgcc tccctttttt cttagagtaa acaaaatcac
    12001 ttatctgagc aactcataac atctaaaaag aaaaggttgt aaatgagacg tgcagcttta
    12061 agcgataaga agcaattttg aaaaatagtt tttcacaatt tataaaatta ttccttattg
    12121 ccgaaaggct ggcccttttt atgagtatgc caatttttta tatcgaatgc tcaaaagatg
    12181 agtgattttt tggttgacta tcatgtttgt gtactgaata ggtccagtag aacagtattt
    12241 ttacgacttg ataattaagg gatgaaattt tcagaaatat gacgtataat gtaaaatact
    12301 gaaaagttgc gaccttcaac tcgtagatgt ttcagaatct aattgaaatg atggtgatat
    12361 catgacagac aacagcaaaa cacgcgttgt cgtagggatg agcggcggtg tcgactcatc
    12421 tgtaacagca cttttgttaa aggaacaagg atacgacgtg atcggaatat tcatgaaaaa
    12481 ttgggatgac acggacgaaa acggtgtatg cacggccacg gaagattata aggatgtagc
    12541 gaaagtagct gctcaaatcg ggattcctta ttactctgtg aattttgaaa aagaatattg
    12601 ggatcgtgta tttgaatatt tcctagccga ataccgcgca ggacgtacac ctaacccaga
    12661 cgtgatgtgc aacaaagaaa tcaaattcaa agcattcttg gattatgcaa tggatcttgg
    12721 cgccgattat gtagctactg gacattatgc gcaagtcact cgcgatgaaa acggcgtggt
    12781 ccacatgctt cgtggagtag acaataataa agatcaaact tacttcctta gccaattatc
    12841 acaagaacag ctttcaaaaa cgatgttccc tctaggcggc atggaaaaat cagaagtacg
    12901 ggcaattgct gaacgcgcag gccttgcgac tgctaagaaa aaagattcaa cgggaatctg
    12961 ctttattggt gaaaagaact tcaaacaatt tttgagcaac tatctgccag caaaaaaagg
    13021 aaatatggtc actcttgatg gtgaagtcaa aggacaacat gctggcttga tgtattatac
    13081 gattggtcaa cgtcaaggac tagggatcgg tggcggagga gattcgcaag aaccctggtt
    13141 tgttgtagga aaagatctag cgacaaacac attatatgtg gggcaagggt tccatcatcc
    13201 tgcattgtat gccacaagct tggatgcaag tgaaatccac tttacgacga atgaaccaat
    13261 gccaaaagaa ttcaagtgta cagcgaaatt ccgttatcgt cagcaagacg ttcctgtgac
    13321 agttcgctta ttggatgata atcgtgcaga agtcgtattc gacgaacctg tacgtgcaat
    13381 cactcctgga caagcagttg tattttatga tggaatggaa tgtctgggtg gtggtttgat
    13441 cgaccacgca tatcaagaaa caaaagtttt acaatacgtt taagcgatat taaatgaatc
    13501 aaaacagcta tggctaacaa aagaaacacc ttttcttttg gatagcatag ctgttttttt
    13561 gcaataaaat taatttttat tcattttata cttgaaatac ttgtgtaaaa agttcataat
    13621 atccaataat atgaataaag ttgctttttg ttaagcaaga gcagttgcaa aatagaaggg
    13681 aatcttttgc aagtgatttg ttcaatttta taagtgaggg tgatggaaat ggaaaagaaa
    13741 tcgacgggta ttagcaaatg gggactggtt gctctggttg tcagttcgtc aatcggaagc
    13801 ggtgtgtttg ggatcacaag tgatctggct agttctgcag ctccagggcc agcgatcctt
    13861 tcatggataa tcgttgggat tggtatttta gctttggtat tatcgctgaa tcatttagga
    13921 gaaaagcgtc cagatctaga tggtggtatt ttcggttacg cggaagcttc atttggtaaa
    13981 ttaggtggct tcatcagtgg ttggggttac tggttgtctg cgtggctggg aaatgttgct
    14041 tttgcaacga tgctgatgag cgccatggga gaatttttcc cgatattcaa aggtggacaa
    14101 aatgttcctt ctattttatt tgcaagtatt tttatttggg gtctgacttt attagtaaac
    14161 aatggagttg aaggagcgag cgtcatcaat acaatcgtga cgatctgtaa attagtgcca
    14221 ttattcctat ttttaatatt tgtatttatc gcatttaaag tgaatatatt cactgctgat
    14281 ttttggggta acgtatctga taatctagtg aatggaacag ggcagcaagg aacgatctgg
    14341 ttgcagatta aaggttgttt gatggttatg atgtgggtct ttgtagggat cgaaggcgca
    14401 agtgttttag ccaaccgcgc gaagaaacgt tcagaagccc agcaagcatc gatcattggt
    14461 ctgctttgtt tattgtttat ctatatcctt gcttcgatct tgccatacgg cgtcttatct
    14521 caagaagagt tagcaactat ttcacaacct gctatggcaa atattttaaa aggaatcgtg
    14581 ggaaattggg gagcagcgct gatcaatatc ggcttgatta tttcgatcat tggttcttgg
    14641 ttgtcttgga cgatgctccc agcagaaaca acgatgttga tggctcgaga cggtctgttg
    14701 ccgaaaggtt ggggaaaaac gaataaaaaa aatgccccaa cttattcgtt agttgttact
    14761 gcaatcttga ctaatctatt cttattgact ttcttagtta ctgattatgc ctatcaattt
    14821 gcctattctt tatgtacagc ggcaattttg atttgttatc tacttgtcgg gatctatcaa
    14881 gtccagtttt ctttaaaaga aggaatcaaa aaacaagtag tgatcggtgg gattgctgta
    14941 gtttttgaat tgatgggcat cattttagca ggattttctt atgtcctttt atgtagtatt
    15001 gcctatcttc ctggtttttg cttctactgg atcgcttgtc gtgaaaccaa acatcaaatt
    15061 acgggaaaag aaaaagtaat gattgggctg atttgtgtgg ccgcactgct ttcaatcggg
    15121 ttgcttgctg taggctggat caagatataa gggggaaaga aacatgaaaa tcgcaaattt
    15181 cggtgtagaa gaatggctaa ataaatggga aaagaaagcg gtatatgata ttgcacaaag
    15241 ctcgatcgaa gcattgacat tagacgaact cgttggctta gatgggacta gcgtaagtaa
    15301 atttttcgaa aaacgcaaaa acgaaccatt ggactatggc tggatcgaag gatcagatgt
    15361 tttcaaacaa gaagtcgctt ctctttatca aaccgtcgat ccggaaaaca tcttacaaac
    15421 gaatggagcg actggtgcta atcttttagc tctatacgcg ttagtggaag caggagacca
    15481 tgttgtttcg atgcttccta cttatcagca attatatgac atccccaaat ctttaggtgc
    15541 cgctgttgac tttgttcatt tgaaggaaga agaagattgg cagtttgatt tagaacagct
    15601 agaagcctta gtcaaacctg aaacaaaaat catctgtttg aatagtgcaa acaatcctac
    15661 aggcacattg ctagatcgga aaactttaga aaaaatcgca gaaattgctc gtacagtaga
    15721 tgcatatgta ttgatagatg aagtatatgc gcctttaacg gataaaggtg aattcttgtc
    15781 gatcgtagat gtctatgaaa aaggtatagc aaccaattcc ctatccaaaa cctactccat
    15841 tccaggtatt cgtattggct ggacagctac tggacctgag ttggcagaag tttttcgaaa
    15901 gtatcgagac tacacgatga tttgcggcgg agtcctgtca gatgatcttg ctgtacatgc
    15961 attgaagaat aaagaaaaaa tattggcgcg aaacaaaaag atcattacag agaaccttgt
    16021 gattttaaaa caatgggtag ccaatgaacc gaaagttgaa cttgtcgctc ccaattacgt
    16081 ctctacttct ttcattaaat tagccatcga agaagatgac cagacatttt gtatcaattt
    16141 attagaagaa acaggagtac tattagtacc aggaagtgcc tttgatttac cgaagcatgc
    16201 ccgactaggt tactgttgta aaaaagaaac cttggaaaag ggattattcc tcttatctga
    16261 gttcttgaaa aaacaggcct aaacaaaaac actgcaagga cagtctgtga caactgttca
    16321 agcagtgttt ttcatttacg cttattgtct atgagaagga gagtgccctg aagctatgga
    16381 gactcctgaa gctttttctt ctgcagtttc ttgctgatgg atctcttgtg tattgtgtcg
    16441 tacaagcaag aagttgacca aaacaagaag agaagtcgaa agaaagacgc cgcgataacc
    16501 aaatgcactg gaaacactgg agccgatcat aggccctact acgttaccag ttgcttggaa
    16561 agattggtta taactgaaga tccgtccagc agcttgatga ggcgaatatt ttgtgatcaa
    16621 tgcttggaca gcagggagca gacaagcatc agagatacca atcaaaaaac gtaaggcagc
    16681 taattgccaa acattcgtga cgaatgccat tgggatataa accagaatcg caaaacccaa
    16741 accaatcgca agtattcttt cactcccaat cttgtcacct aagcgaccaa atcttggagc
    16801 ggctatcaaa gtggcaatcc caggtatcga agcaatgatt ccgctgatca acgtaacatt
    16861 cccatgtcca tgcagcaatt ggcgaatgta cagactgatg attggactaa tggaattgtt
    16921 ggatgcttgg atgatcatcg ttgtgacgaa cataccgatg acgacgtgtg gatatttcaa
    16981 gtctttgaaa atctgtttgg cagaaaccat gttttctttt ttcactggga caaatttttc
    17041 atggacgaaa aacagactca acaagaagac gaaaaataag atgatccctg taatgaaaaa
    17101 ggtagggcga tagccaaacg ctgaagcaga gatcccgcca agaagcggtc caagtaatgt
    17161 tcctgtaaca gaaccagttg ctaaggttcc aagaacttga ccactttttt cttttggcgt
    17221 cccagttgcg acaagggcag tggcattact gatataacca gaaaaaatcc cttgaagcag
    17281 acgcaatgcg actaattgat acacactagt cacgcaaccc atcaaagaaa tgacgattgc
    17341 cattcctaat gaagcacgca acagcatcaa ttttcttcct ttttggtcgg ctaattttcc
    17401 ccaccaagga gagataagcg tagtcaccaa gaaagtagat gaaaaagtca atccgctcca
    17461 aaagttcaat tgtgacgtac tataattacc taacgtatct atgtaaagtg acataaaagg
    17521 catgactaaa ctaaatccga taccagccat aaatgttcca aaccataaga caaacaagtt
    17581 ttgttcccaa gtttctcttt tcctaaataa tttttccttt attcttgcag ccaattagac
    17641 tccttctttc tgaaaatttt catctacagc tctttattat aaactacaat atgtagttta
    17701 tcaatgaaag tctctttttt ctctttcttt ttcgtggtaa actgaaagag tgaaggattt
    17761 attttatgag aggaaacatg gaaatgaaaa aaagaaatct gtctcaagag aaaatcatcg
    17821 actgctttcg agaactagca gaagagatgg atgttcagca aataaccttt cagcatttag
    17881 ccaaacgctt ggatatcaag gctccctcgc tgtataacca tttcaaaaat attcgtgatg
    17941 tcaaaacagc gctaacagcg aaacttctgc aggaacttaa tgaccagctg cgacgggctc
    18001 ttgtgggaaa aagtggtcag gaagcactgc agatctatgc agaaacttat cgatcctttg
    18061 ctttttcaaa tcaagcagtg tatgagttac tgatcagtgt accgcatacc aatgaagctg
    18121 ttttattaga tggaatccat gaaacgaatc agatcatttt gcagatattt gaggcttttc
    18181 aattaacgaa agaagaaaaa gttcatcgaa gcagggaact tcgtagtatg atccacggtt
    18241 atctttcctt acgtttttta ggatacttta caaaagaacc aacaatcgat ccagaagaca
    18301 gctatttttg gatgatcaat aactttatcg ctactttgcc ttaaacaatc agattcattt
    18361 gtaacaattt taagatgttc attaaatgac ccttttataa gagaaaattc ttgcaacttt
    18421 cttgaaaagt gactaagatg agtctagaaa agctgaaaga aggagcacga gtatgtatca
    18481 agtaattacc atgttcgggg acaatgagcc ttggtggttt tttgaagact gggaagaaga
    18541 tattgaagaa gaagaaacat tcgagtcttt cgaagaagca cgacaagcgt acgaaaaaca
    18601 atggaacgaa atccatcaaa attatgaata tatcaatgca aaaagtaact ttctcagcgc
    18661 cttttggaat gacggagatg agcgctggtg tgaagagtgt gacgatgatt tacagcaata
    18721 caagggactt gcgttgttga aaaatcatca gcccatcaca gtagaaagca gaaaggaatt
    18781 ttatgaaaca actaattcta gcggaaaaac caagcgttgc gaaagaccta agcaaggtgc
    18841 ttggtgctaa tcaaaagcac aaaaattatt atgaaggacc caaggtgatc gttacttggg
    18901 cccttggtca tttattagga ttgaaaatgc cggaagatct gaataaggaa tggcagagct
    18961 ggcaaatgga cacgttgcca atgatcccta aaaaactagg aatcaaacca cttcctaaaa
    19021 caggacatca attaaaagca atcaagcagc tggctcagcg aaaagatgtc tcagaagcag
    19081 tcattgcgac agatgccgga agagaagggg aacttgttgc tcgctggatc ttagagtatg
    19141 tgcatttcaa taaaccagtg aagcggttat ggatctcttc tcaaacagat aaagcaatca
    19201 aaactggatt caagcaacta agacctgcga aagaatatga tgcattgtat gaatctgctc
    19261 ttgcccgggc aaaagcggac tggctggtag gattgaatgt gaccagagcc ttaactgtga
    19321 agtacgcgga taatttatca gcaggccgtg tgcagacacc gacactagca ttagtgagag
    19381 accaagaaaa gaaaatcgag cagttccgtc cacaaacgta tttcacgatc acgttgcaag
    19441 ctggaaaaga acaagccaaa atgcttcaaa aaaatcaatt tgctttaaaa acacaagaag
    19501 aagcacaaaa acttgttcaa ttgatcagta caagcaaagg aactgtcaca gaagtccaag
    19561 aaaaattaaa aacggaatct gcgccattgc cttatgattt aacagagatc caaagagaag
    19621 ccaatcaacg ctatggctat tctgcaaaga aaacattaag tcttgtacaa agtttgtatg
    19681 agacccataa gatcgttact tatccgcgta cagacagcaa atatctgaca aatgacatga
    19741 aaagtacgat gaaagaacga ttgcaagcag tcagcgactt tgctcctgag gtcaagcagt
    19801 atttgaaaaa cggagccatc gtccaacaga caaaagtatt ccaagacagt aaagtgaccg
    19861 atcaccatgc acttttgcca actgaaaaca gagcacgtta tgaaaaatta agtaatgaag
    19921 agcgaaaaat ctatcaaatg attgtctctc gttttttagg actgtttgct caaccccata
    19981 aagtcagcca aacaaaagtt acggttgaat ttggtaaaga acgattcatt tttcggcaaa
    20041 atcgtgtcgt ccaagcagga tggaaaggcg aaactgaaac tgagacggaa acagtcaaat
    20101 gggaaaaagg aatgagaatc aatcccgatt tcgccatcaa aaaagaacta tctgcaccgc
    20161 ctaaaccatt gtcagaagca agcttgcttg gtcaaatgga aaaatacagt ctgggaaccc
    20221 cagctacgcg tgcagagatt atcgagaaac taatcaaatc tgaactgatg gaacgtaccc
    20281 ctcaaggact gcaagtatct cctaaaggta agcaattatt agtattggta aatccctctt
    20341 tagtaacacc agatttgaca gaaagctggg aaaaagacct tgaagctatt gctgcaggaa
    20401 agaaacaagc aggaaccttc ttgaatggaa tcgaaaaaga aacaaaacgc ttggtgaatg
    20461 agatcaaatc aagcaaacaa gaatatcaag atttctcgat tacccaaaag aaatgcccag
    20521 aatgtggggc caatctgcgc gagaaaaaca cacgtgacgg caaaatctat gtctgcacca
    20581 attcagattg caactaccgc agaagaaaag atccaaaagt ctctaatcat cgatgcccgc
    20641 aatgtcaccg gaaaatggaa atcatcgaag gtaaaaacgg tgcttatttc cgttgtaaat
    20701 acgatggaac gacagaaaaa ttgcttgata aaaaagagca gaagaagaaa atgaccaaac
    20761 atgaagaacg acgcttgctg aaaaaatatg cacaagcaga agaaccagaa gaaagcccat
    20821 tagcagcagc actaaaagca gcaatgaaga agtaataaga tatgaaagag gttgtgatag
    20881 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    20941 aaatttcggc ttatttccga agaagctact tctggaacac catttattcg gaaaaaagga
    21001 ctgtgacaag acttttgtca cagtcctttt tcatttaaaa aaatgtttac ttcatttgac
    21061 gtatattatt ggtgaggctt caattcattt ggcttcactt tttctgtttc tcttatgata
    21121 taaacaggac gctgtttcgt ttctaagaag attttcccaa tatatttccc gatgattcca
    21181 agacataaca attggattcc accaatgaac aggaagatcg agaccatgga aggccatcct
    21241 gatgtaggat caccaaacaa caatgcccgc aaaataatga acaaaattgc aataccagaa
    21301 ccgacacaag acaaagcgcc gataaatgaa gcaatcgtta acggtgcatc cgagaagttc
    21361 acgataccat caatcgagta gttgaataat ttccagaagg accaagatgt ttcgccagcc
    21421 acacgttcgc ggttttcata agaaatatat tctgttttaa atccgaccca gctaaataat
    21481 ccttttgaaa atcggttgta ttctgttaat tctaggacgg catccaccat ttgacgagtc
    21541 atcaagcgga aatcacgagc gccatcgacc atttctgtat cgctgatttt attgatcaac
    21601 tggtagaatt ttttcgcaaa gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc
    21661 cgggtgccga cacaatcgat atcagaagta gtgatccggt tgatcatctc aggaagcagt
    21721 tcgggcggat cttgcagatc aacatccatc acggtcacta aatctcctgt agaagcttgc
    21781 aatccagcgt acagagccgc ttctttccca aaatttctgg aaaatgagac aaagcggaca
    21841 taaggataag ctgaggctag cttcttgatt gctgctaacg taccgtcttt tgatccgtcg
    21901 tcaataaaca aatattcgaa ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa
    21961 agaggaacag ttttttcttc attaaaacat gggacaacaa ttgatacttt catcgtttct
    22021 atcctttcaa tcgtatgttt atatatggtt tacattcaaa atttaataaa ccttaaataa
    22081 cacctttaaa atttgatttt acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt
    22141 tagtttacca tgtttcctcg gtttatcaaa cttttaagaa caatttttat tagttggcaa
    22201 gtagttttgg atagagaaaa aggaggaagt tccagtctat tgactaggag caaacaaaat
    22261 gaaaatacac acacctatcg gctttttatg gttagatgca gatgaatccg gattgactgc
    22321 tgtatctttt cagccaattt cagaaagcga atcagcgaat cagcgaattt taacgcgcac
    22381 acaagaacag tttgaagaat attttgcggg aaaaagaaaa gaatttgatg ttcctttatc
    22441 tataaagaga ggtacgcctt ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta
    22501 tggagaaatc cgaacgtata aagaagtagc cgttgctgtt gatagtccga aagctgtgcg
    22561 agcgattggg caggcaaatc gaaccaatcc attgccaatc gttattcctt gtcatcgagt
    22621 gatcgggcaa aatggaaaac tgacaggata tatgggaaac gcaatagatg gggttgaaat
    22681 caaacgccaa ttgttgttgt tagaaggata tctgcaaaat taaaaacagt tgtgattcgg
    22741 acattgttcc atgcgagaat cacaactgtt ttttcatgtt cattctaaac tttgatcagc
    22801 caaataaata gtaaagccat cactgtttgg agaagcccga cagctaatcc ataagataag
    22861 aagcgtgtac cttctttcat gaattttgca aagtctaaac gcagaccaat tgccgctaaa
    22921 gcagtgatct caaaccagct gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa
    22981 gaaaacaagc tgttgaccaa acaaaagatc acaaacccta atacatacca aggaagcggt
    23041 aattttcgtt ttttctgcgt atcagttggc gtttcaaaca gagctggttg tttcttgtgc
    23101 atgaattttt caaacagata gacaacaaca acgagcatca taatacgtag aatcttaaat
    23161 agcatggcga attcgacaac agaattgtca agtaagcttg ccccagcgac tacttgacca
    23221 acagattgga gtgtaccacc caaaagtgca cttttttcaa tgagatttcc accaaacaac
    23281 tgtaatccaa gaaaagggag agtcaacatc atcacagtac ccagtagatt gactagtgtg
    23341 atgatctgtc ctttttcttc ctctttcgca tggatggaag gggcgattga agcaatagca
    23401 gaagaaccac aaacagcgtt tcctccagcc atcatcaaag aagaagtatc gctgaattgc
    23461 agttttttac caatcaagta agcaccgatg atagtgactg acatttgcag taacacaaaa
    23521 acaattcctt ttacaccaat ttgagcaatc gtttgagctg tgacagttgc acctagtaaa
    23581 aaaacagaga attctaatag tttgctttca gcaaattttg tgccgattgc tagaacaggc
    23641 tgtttaaaaa aagtatttcc taataaaata cccaataaaa tagcaatagt cgctccacct
    23701 aactgtggca gataaatagc aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt
    23761 aaaccgggta agatttcgcg gaccgctgaa tcctttacag attttattgg atgtgacaat
    23821 gatttcattt gatcggttcc tttcgtcaat aaatactaaa agcatactcg aacatagtag
    23881 cactaaacca gtattttgac aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc
    23941 ttgtttcgaa taaacggtgt tccagaagta gcttctacgg aaataagccg aaatttcaca
    24001 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttcttt
    24061 tacgacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccagt
    24121 acaaaaacat gaacagctgt ttttgtttgg ccgttcttat tacttgatgc agaacagctt
    24181 tttcacaacc tattgaatcg attattaagc gattttttta gaaacaaatt ttccatgttt
    24241 gctatgttct ttaattaaga aacgtacgta atgagcaaga acgtagaaga caactacact
    24301 tgcaaacaga cattccgcaa cttttaagaa atccaatacg ctactataga tacctaaact
    24361 gttgaagtaa tgaacagtag taaatatagc tgttgcagag ataacaagcg ggaatgtaaa
    24421 ggctgcatag cttggataaa aacgaagctt gaacaaacgt ggaagcatag acagcacaac
    24481 gaaatacaat acttgagata aagcgaacaa tgctacgaca agataaaaat taggttcagc
    24541 aaagttttta agataacctg ttaggcaaag agaaccaggt gctgcaacga tcgtaatcag
    24601 cggacgtgtc ggatgagcca tatttcttac gaaaaagaca cgtttgatga tgatcggcaa
    24661 taagaacgca tagcagatca atgaaatcca aaagaaaatt tttccaataa gtgggaagaa
    24721 gttcccggca gttacagtga tgaccccaaa tccaacgtaa atgataaacc agcttggata
    24781 aattgcttca attgaaagat cagctttgat aacatggtga taattgaaat aaaagacaag
    24841 gatcacttgg aagataacag cgcacagcca aatgaatttg acaattggtg caatactttc
    24901 atatgaaaca aaataagtac aaataaccat gagtgacatc gtaaaagttg gtgaaacgga
    24961 ggcgatgatt ggatcatgca acgtttgttt cgtgtgttcg aataagataa acaatttaac
    25021 aaaaatgaat atcataagaa tcatagagat accgccagtt agattgccta aagcaacatg
    25081 gtgatagtct ttaaacagat ttccaaggga agctaaccca aggatcaacc cgcagatcgg
    25141 gatcggaata attttcaaaa agttcttcat atataatatt ctccttttta tttaatagat
    25201 tttagtacca agatgaacga atacattgta aaatgaatag gaatgtatga ttacgagcaa
    25261 gcagacagct gattgtcttc atctaagtta ctagttggat cagcaactgt tttgttgtac
    25321 atttctatta tagatggttt tttgtatatt taaatcaaat agtttttata gtaattatca
    25381 taaaaagtta tggaagtggt gccatgttta agttattaac gacttttcga gcagtatatg
    25441 aaacaagaaa tttttccaga gcagcagaag tactgttttt atctcaacca gcagtctcga
    25501 atcaaatcaa acagttggag cgggaattga atatcgatct ttttcaacgt aacggaagaa
    25561 aagagatgct tccaactaag caagccgact tattatatga acggacactg gttttgttag
    25621 aagaatggga agaaactcag caaaaattgc tcaatgaaca aaatgaagaa gaagtatgtc
    25681 ggatcgttgc ttcccacacc tttgctgtct atcttttgcc tcagttgatg aaacatctga
    25741 tcaaaaggtt tcctaaagtg cattttgaga tcgaaatcac gaattctcat gaatcattag
    25801 aacaagtagc gaaacatgaa tccgattttg gttttatcga aaagccatta gagacttccg
    25861 gcgtacaaag atactctttg atggaagacc agctagtcat cgctggggac ccaaaaagca
    25921 aattgtggct tgtaagagaa gcaaactctg gtgtttttca ttacacgcag cgttattttg
    25981 aagagatgaa tatccaagat aagaagatga tcgtgaaaag taacgatgta attgttgcgt
    26041 tgctgaagga aggaatcggt cgctcgattt tgtctaagcg ctctgttcca gaaaatgtgc
    26101 caatgactcc gttgaatttc catcgatatt tttatttcat ccaacgaacc aatctggttt
    26161 ctgatcagct gctagagatt gccgaagaga tccttcgata caatgaaagc acagaaactt
    26221 ctcacagcga ggagaatctc agctggtaga taattgcatg tatcaaaaaa agttctatga
    26281 tacactaaag ataagcagta ggcttttgta ctgcatttgc tcaagcaact gcacaaacgg
    26341 aaagttaatt tttcccaaat gtctatggaa agactttcca ttatctaact ggaccgctat
    26401 aataatcagc agtatggttt ttaagaaaag ggatgaaaca tggaaaaaaa attaaaagct
    26461 tcgaacgata atcgtattga ttatggcgtg attttaccag tctttctgtt atgtttgatt
    26521 ggtctgctct cattatatgt agcacttagc catgatccga acaatccagc agtcttgaaa
    26581 ggcgtaggga tgcaagttgt ctggtattta gttggagcgg cagcaatcgt tgtcatcatg
    26641 catttgaatt ccaaatggat ctggaaactg actccttacc tttatggttt ggggttagtg
    26701 gtaatgggac tcttgctcaa attctatgat cagaatttag ctgcttcaac agggtctaaa
    26761 aactggtttc gattcggtgc gtttactttc cagccagcag agttgatgaa gattgcgtac
    26821 atcttgatga tggcactgat cgtaactaaa cataatacgc aagtaaaaga acggacgatg
    26881 aaatcggact tttggctgat tgggaagctc ttgatcgtga caataccggt tttagcattg
    26941 atcatggccc aagatgactt tggaacgatg cttgtctttc ttgcgatctt tggcgggatt
    27001 ttcctgatgt ctggaatctc ttggcgcatc atcgcacctg tagtaatctt ggcagtagtc
    27061 gttggagcag gaacgatctt cttggttacg actgaaggcg gaagagactt gctttataaa
    27121 gttggattca aatcttatca gtttgcacgt attgattcat ggctggaccc tttccatgat
    27181 acgtcaggaa tgagttatca gccggcccaa ggacttttgg caattggtac cggtggcttg
    27241 tttggtaaag gattcaacgt aagcaatatc tatgtgcctg ttcgtgagtc cgatatgatt
    27301 tttaccgtta tcggtgaaaa cttcggcttt atcggtggtg cattcgtcat tttcttgtat
    27361 tttattttaa tctatcgtat gattcgggtg tgtttcgata cgaataatga attttacgcc
    27421 tatatcgcaa gcggtttgat catgatgctg ctgttccatg tctttgagaa tattggagca
    27481 aatatcggat tacttccttt aacagggatc ccattgcctt tcattagtca gggaggatcc
    27541 tcgattttag ggaacatgat cggtatcggg ttgatattat cgatgagata ccaaaatgaa
    27601 gcacctgcaa gatcttcaag gaggtaagaa aattgtacac cttttactgg tacccgaaat
    27661 gttcaacatg caaaaaagcc aaagcgtggt tagaccagca tggcgttagt tatcaaaccg
    27721 ttgatatgat caaagaagta ccgaaggcct caacgattga gacttggcta gaaacgagtc
    27781 aagtaccaat ccgtcgcctg ttcaacacaa gcgggatgaa atatcgggaa cttggactga
    27841 aagatcaagt aggcgagttt acggcttcaa aagcgagcca agtcttatcg acagatggta
    27901 tgctgatcaa gcgaccactg atcttacgag aggaccagct tgttgcaatt ggattcaatg
    27961 aaaaaattta tgaaggagaa ctgatataaa atggcaggca agcatttgaa gaaaaaggac
    28021 aacctttgga tcttatttaa tggaaaagaa tactgtgttg gtttgacaaa tgaagcgcaa
    28081 gaagaacttg gagacatcac gtttgccaat ctgccaaaag caggacaatc gctaaaaaaa
    28141 gatgaacctc tgatcgaagt tgaagcggaa aaagcggtca atgaatttgc cagcccgcta
    28201 acgggtacag tttcttctgt taatgataaa atcggtgaag atatcaatat cttgaatgat
    28261 tcagatgaaa tgaatgcgtg gatcttaagt ttcaaagatg ttgatccaaa agaatttgac
    28321 gaattataaa atcagaaaat cacttgaaaa aagttgacac tgattcttcc agttgctata
    28381 atcataagca atcctatatg aaacgagaaa gctttgaaaa gaagagtaca tgtcacggac
    28441 gtttgaagag agccctgttt gctgaaaatg ggtaacgaaa atgagatgga agatggtctt
    28501 agagcagagt aagcgagcgt aagcaaactt actacgggag tgcccgttac agcgcgacag
    28561 tatacggaaa gaagctaagt ccaagtactg gaagaggctg ttattgcgaa gtaacggtaa
    28621 atcaaggtgg taacacggaa gttcaagctt tcgtcctttt gcttaaggca agagggcgaa
    28681 ggcttttttg tttgaaaaat tataagagaa agatgtgaat ggcatgtcat taatccaact
    28741 aacgaatgtc aaaaagacat tcaatgggaa aaacggaaac atccaagccg tcaatgacgt
    28801 ttcactaaac gttgaaaaag gggatattta tggaatcgtc ggctattctg gcgcaggaaa
    28861 aagtacatta gtccgtctcc tgaatggact tgagcttccc acgagcggcg aagtgatcgt
    28921 aaacgatcaa gatattacaa aattaaaaaa caaagaatta cgtacattcc gaaagaaaat
    28981 cggtatgatc ttccagcatt tcaacctgtt atggtcaaga actgtacttg aaaatatcca
    29041 gctgccgctg gaattagctg gcgtaccaaa gagcaaacgt aaagaacggg cagaagaact
    29101 gttgcgttta gtcgggttag aaggacgcgg acaagcatat ccttcacaac tttccggcgg
    29161 acagaaacaa cgcgtcggta tcgcacgagc attggcaaat gatcctgaga tcctgctttg
    29221 tgatgaggct actagcgcat tggacccgca aacaacagaa gaagtattag acttattgtt
    29281 agcgatcaat aagaaattga atttgaccat cgtcttgatc acacatgaaa tgaatgtgat
    29341 ccgcaaaatc tgcaacaaag tcgctgtgat ggagttgggg aaagtcgtag aagaaggaga
    29401 cgtattgact gtcttcagac aaccgaaaaa agaagtaacc aaacgctttg tacaacaaga
    29461 catcgaacca gaagctgatt cgacagaact attagctgaa ctcatcaaag aaaatccaag
    29521 cggccgtttg gcaactttga cctttagcga aagaaatgcc aacgagccag tcatttccca
    29581 agcgattcga cgctataaca tcgatgtcaa cgttgtgtat ggaaagatca aacaagtgaa
    29641 agaaggttcc tttggttcat tgactgtctt gatgactggt gaaaccaaag aattagatca
    29701 agcagaagtg ttcatcaaag aacaaggagt aggaattgag gtgatccatc gtggataaaa
    29761 gcttttcaga aacatatcta gattttaaac aaatcaaccc ggaaacgatg aatcaggcta
    29821 tccttgatac actatatatg acattaatct caatggtctt agtgacgatc atcggattag
    29881 ttttgggatt gatcctttac tctattggac gcaaaaacaa accgtatgct cgaatcattt
    29941 atggaatcgt atcgatcatc agtaatattt tccggtcaac gccatttatg atcctgatgg
    30001 ttttgatcat tccatttact aaagcaatcg taggcactat gctaggtgcg caagcagcca
    30061 ttccagctct tgttttatcc gcagcaccat tttatgcccg tctagtagag attgcttttc
    30121 gtgaagtcag ccctggtgta ctggaagcag cagatgcaat gggagcaagt tactgggaaa
    30181 tcatctggaa ggtcttgata cctgaaagta ttcccgcatt gatttctgga ttaacagtta
    30241 caacgatctc gatgatcgga tttactgcga tggccggcgc gatcggcgca ggtggacttg
    30301 gaggtcttgc ttggcaggaa ggctaccagc gtggtaatct aacagtgaca ttcgttgcga
    30361 ctttaattat tttactaatc gtatttatcg ttcaaggaat cggtgatttc ttaactaaga
    30421 aaacagacaa aagataatag gatatagggg gatgtaggat atgaaaaaga aaatttttgg
    30481 atttgcagca gcgcttttgc tgactgtagg attagcagca tgcggtaaca acagcgacag
    30541 caaagattca gcaaataaag cagacgacac gacattgaaa gtcggcgctt caccgacacc
    30601 gcacgcagag attttggaac atgtgaaacc attattgaaa gatgaaggaa tcgatcttga
    30661 aatcgtcaaa ttcgatgact atgtattacc gaaccaatct ttggaagaag gcgacattga
    30721 tgccaattac ttccagcata ttccttattt gaacaaagaa atcaaagaaa aaggatatga
    30781 ctttgtcaat gccggtgctg tccacatcga accaatgggc ttgtactcta aaaaagtcaa
    30841 agatgtttcc gaattaaaag atggtgcaac aatcattact tcaaattctg aatcagactg
    30901 gggacgcatc atcacaattc tacaagatgc tggcttagtc aaagtcaaag atggagtaga
    30961 tctggaaaca gcaacattcg atgatattga agaaaaccct aagaacttga agttcaacca
    31021 tacgatcgac ccatcacttt tagcttctac gtaccaaaat gatgaaggtg acctagttgc
    31081 aatcaacgca aacttcgctt acggcgcagg gttgaatcca ttagaagatg ccgtattgct
    31141 tgaaaaggac aactctcctt acgtgaatat tattgcgact cgtaaaggtg atgaagacag
    31201 tgacaaaatc aaaaaactga ttgaagtgct tcaccgcaaa gaagtccaag aatggatcac
    31261 agacaaatgg ggcggctctg ttaaaccagt aaaggctgat gcaaaataag gtatggaaca
    31321 aaaaagatga gaaataagtc tgtaatggct tatttctcat cttttttata cctattctaa
    31381 tttagaaatc aaaattgttt aataataaat aaaagatatt tgattttagt aaggaaggtc
    31441 attgcgcttc attctcaatt agtctaaaat gagtgagaac gaaagagaat ttgggggtat
    31501 aatgcatgtc tgtcttagaa ataaaaaatc tacatgtgtc gatcgaggaa aaagaaatcc
    31561 ttaaaggcgt aaacctaaca atgaaaaccg gagaaatcca tgccatcatg ggacctaacg
    31621 gcacaggaaa atctacctta tctgccgcta tcatgggcaa tccaaactat gaagtaacag
    31681 aaggcgaaat tttgtttgac gggaaaaatg ttttagactt ggaagtagat gaacgtgcgc
    31741 gacttggctt atttttagct atgcaatatc caagtgagat accaggtatc acgaatgcgg
    31801 aattcatgcg tgcagcgatc aatgcgaaac gtgatgaaaa cgacaaaatt tctgttatgg
    31861 catttttgaa aaagttagat gaaaaaatgg ctttactaaa tatgccagaa gaaatggctg
    31921 aacgttatct gaacgaaggt ttttctggcg gagaaaagaa acggaatgag atcctgcaat
    31981 tgttgatgtt agaacctaca tttgcaattt tagacgaaat cgattcaggt ctggatatcg
    32041 atgctttgaa agtcgttgct aaaggagtaa atgaaatgcg cggagacaat ttcggtgcat
    32101 tgatcatcac ccactatcaa cgattattga actatatcac acctgatgtc gttcacatca
    32161 tgatggaagg ccgagtagtc atgacaggga atgctgacct agctaaacgt ctggaagcag
    32221 aaggctacgc tggtatcagt aaagaattag gaatcgacta taaagaagaa gaagcgtaag
    32281 ggggagcaag aatgaaagaa aaaaactggc tcgattatct tgacgcggta aatgattttt
    32341 ctttaagtaa aggcgaacct gattggatga gaaccttccg tcaagacgca ctagcgaaag
    32401 cagatgaact gccattgccg catatcgatc gggtgaaatt ccatcgctgg tcgttatttg
    32461 atgtgaaaga aacacaaacc atcagtgaaa caggcacgat tccggcattt gatgcaatga
    32521 aagacaatcc tgtgcttgtc caacaaggtt catggacgat ttttgaacaa ctgcctgtgg
    32581 aattagcaga aaaaggagtc atttttaccg acttgttcac tgcaatgatc gaatatccag
    32641 aactagtcca agaatattat atgaaaaaag cagtaaacat gaacgaagac caattgactg
    32701 cactccatgt agcttttatg aacagcggta ttttcctgta tgtgcctaaa aatgtcgtga
    32761 tcgatgaacc actcgagtcc ttattcattc aagatggcgc atccgatgag cacttcttca
    32821 agcatgtgtt gatcgtggca gatgaacata gtgaattttc ttatctggaa cgtttccaga
    32881 caaccaaaga acaagtagca aaatcatcag gtaatatcat cgtggaagtc atcgctaaag
    32941 ctggttcaaa aatcaaatat tcagccgtag atcaattagg agaaaatatt acttcttata
    33001 tgaatcgccg tgggcacatt ttacgagatg cttctgtcga ctgggcaatt ggtgtcatga
    33061 atgacggtca tgtgatcgct gactttgact cagatttagc aggagaaggt gcacacgcag
    33121 aagtcaaaat cgtggcaatc agctcgggca gacaaatcca aggaatcgat acacgagtaa
    33181 ccaataaagc ccctcatacg atcgggcata ttttgcaaca cggagtgatc cgagaaaaag
    33241 gaacattgac ctttaatggt atcggccaca tcttgaaagg ggcaaaaggt gcagatgccc
    33301 aacaagaaag tcgtgtcttg atgctttctg ataaagcacg tggtgatgcg aatcctattc
    33361 ttttgatcga tgaaaacgaa gtaactgctg gacatgctgc cagtgtagga cgagttgatc
    33421 cagaagaaat gtactatctg atgagtagag gattgcataa agaagaagca gagcgcttag
    33481 tgattcgagg attcctcggt tctgtcttaa cagcaatccc tgtagaacaa gttcggaaag
    33541 aattagtcga agtaatcgaa gggaagttga atggatgatc gatgctaaaa agctacgcaa
    33601 tgattttcct atcttatttc aagacgtcaa tgatgaacct ttagtttatc tggataatgc
    33661 agcgacgacc caaaagccaa aacaagtact ccaggcatta gcgtactatt acgaacacga
    33721 taacgccaat gttcatcgcg gtgtccatac gttagcagaa cgcgcgaccc atgattatga
    33781 acaagcaaga gaaaaagtac gggcgttcat ccatgcaaaa gaaacagcag aagtgctgtt
    33841 tactcgtggt acgacgacaa gcttgaattg ggtagccaga accttcggcg accgctatat
    33901 caaagcagga gacgagatag tgctctctta catggaacat cattctaatg ttatcccgtg
    33961 gcagcagttg gcaaaacgaa caggtgctgt cttgaaatat atcgaactaa cagaagaagg
    34021 cttcctggat atggaggatg cagcgaaaaa gattacggac aaaaccaaaa tcgtctccat
    34081 tgctcatgta tccaatgtct taggtgtctt caatcctgtt caagaattag ctgaacttgt
    34141 ccatgaaaac ggcggggtca tcgtagtaga tggtgctcaa tctgccccgc acatggccat
    34201 tgatgtccaa gcactagact gtgatttctt tgctttcagc ggacacaaaa tgtgcggacc
    34261 aacaggtatt ggtgttttat atggtaaacg aaaatggtta gaagaaatgg aacctgttga
    34321 atttggtggc gaaatgatcg attttgtcga attatacgac agtacatgga aagaactacc
    34381 ttggaaattc gaagcaggga caccaaatat cgccggcgct atcgcattag gccatgcaat
    34441 cgattatttg caaaaactcg gtatggacaa tatccatcga tatgaagaag aactagccgc
    34501 ttatgtgttg ccaaaattac aagcaattga tggattgact gtttatggtc cacaagaccc
    34561 gaattttcgg acaggagtcg ttgcattcaa tttggatggg ttacatcccc atgatgtcgc
    34621 gacagcttta gacatggaag gggtagccgt cagagcaggt catcattgtg cgcaaccgct
    34681 attgaagtac cttgaagtat cagcgactgc tcgcgcgagc ttttatttct acaacacaac
    34741 gcaagacgcc gaccgtttag ttgaagcaat catggcaaca aaggagtttt tccaacatgg
    34801 gactatctaa attagacaat ttataccgtc aagtgatctt ggatcattct tcccatccgc
    34861 atcatcgtgg aagcttgact gactcgacaa gcaagatcga attgaacaac cccacatgtg
    34921 gagatgtgat cgaattgcaa gtcgatgtaa aagacggaat catcaaaaat atcgcctttg
    34981 acggcagtgg ctgctccatc agtacggcaa gtgcctcgat gatgacagac gccgtcatcg
    35041 gaaaatcaat agaagaagca gaacatttaa ctgtagactt tctacagctt gtccaaggaa
    35101 aagaagtcaa agatcccgac caattaggcg atgctgctat gcttggtggt gtagctaaat
    35161 ttcccgctcg gatcaaatgt gcgacacttg cctggaaagc actaggaaaa gcaatggaaa
    35221 acgatggtac cgcagctttc catcattacc acaaggaaga ggagtgagaa catgggcgta
    35281 ccagaattag aagaatataa attcgggttt cacgatgatg tcgaaccggt tttcagtaca
    35341 ggagaaggct tgacagaaga agtgatccgt gagatgtcaa gaatcaaagg cgaaccagaa
    35401 tggatgcttg aattccgtct gaaatctcta gaaacattta acaaaatgcc aatgcaaaca
    35461 tggggccccg acctttcaga catcgatttc gatgccatca aatattacca aaaaccaagt
    35521 gacaaacccg ctcgtgactg ggaagacgta ccagaaaaaa tcaaagatac atttgaaaag
    35581 attggtatac cagaagctga acgagcttat ctagccggtg cttccgcaca atatgaatca
    35641 gaagtcgtgt accataatat gaaagatgaa ttcgaaaagt taggaatcgt ctttactgac
    35701 actgactctg ctttgaaaga ataccctgaa ttattcaaag agtacttttc aaaacttgta
    35761 ccgccaaccg acaacaaatt agcagcgctg aattcagccg tatggtcagg tgggaccttt
    35821 atttacgtgc caaaaggcgt caaagtcgat gttccattac aaacgtactt ccggatcaat
    35881 gcggaaaaca cggggcaatt cgaacgtacg ctgatcattg ttgacgaagg agcaagcatc
    35941 cactatgtcg aaggttgtac ggcacctact tattcaagta acagcctgca tgccgccatc
    36001 gtagaaatct ttacaagaaa agatgcgtat tgccgttaca caacgatcca aaactggtca
    36061 gacaatgtgt acaacttagt aaccaaacga gcaaaagcat acgaaggcgc aaccgttgaa
    36121 tggatcgacg gaaacctagg tgcaaaaaca acgatgaaat acccaagtgt ttacttagat
    36181 ggaaaaggtg cacgaggcac catgctctcc atcgcctttg caggagcaaa ccaaatccaa
    36241 gatacgggtg caaaaatgat ccacaatgca ccaaatacat ctagctctat cgtctctaaa
    36301 tcaatttcaa aagacggcgg agaagtaaac tatcgaggac aagtaacctt tgccaaaaac
    36361 agtgcgggtt cgatctctca tatcgaatgt gacacgatca tcatggacga atggtcgaaa
    36421 tccgatacga tcccattcaa tgagatccac aacagccaag tctctttaga acacgaagcc
    36481 aaagtctcaa aaatctcaga agaacaactc tactatctga tgagccgagg cctgaccgaa
    36541 tctgaagcaa ctgaaatgat cgtcatgggc tttgttgaac cattcaccaa agaacttcca
    36601 atggaatatg cagtcgaact caatcgattg atcagttatg agatggaagg cagtgttggg
    36661 taaacctgtt ataggagcta gaacccttga tatataaggg ttctagcttt tttgttttgc
    36721 ctgtatacta gaaatgacca gattttgacc agctctttta ctgatgagcc atcatatcag
    36781 caaagtcttg acctgttttc ttaggtgcat tatttgtaat atgtgcatag atgtctaatg
    36841 tcactttact attttcatgc cctaatcgtt gttgaactac cttgacagat tctcctgctt
    36901 ctaataataa actagcatgt gtatgtctaa acccatgagg tgtaatacga ggcaagttat
    36961 attttttcaa aatatatctt aaccaatcat taacagtgtt tggaatgtat agagtgtttt
    37021 taactgttgt aaaaacatgt tgctctttag agctagtatt gtatccaagt tttaaatact
    37081 cttctttttg ctgtaatttc cagttgttga ggatagaaag agtctcagta tctaaagaga
    37141 taactcgtct agaagctctt gttttcggag attgaattat aactttacca aattcatcta
    37201 cagcaagagt tttattgaca tgtagttctt tattaaacgt gtctatgtct ttccactgta
    37261 aagcgagtac ttcactcttt ctcatacctg tgtatgccaa taatctgaag aaggcgtatt
    37321 gtttcatgtt accaaaatct ttaaaagcat ccagtaaatt attaagctcc tcttttgagt
    37381 aaaatttacg atttttctca tactcttttt ttcgtggaag aagtgttttt tccataggat
    37441 tattgtcaat atattgcatt gcaaccccgt agcgtaaaat ttgtgcagtc tgttttctca
    37501 tataatcgta actttcatat ttactgtgcc attcatctac cactttttga caatatggga
    37561 cagagatatt cgatagttta agcttaccaa atgctggaag tatttgatta tttgcgtatc
    37621 ttactgagat tgcgattgtt gaagctttaa cactggtacg ctgatgagtt atccattcat
    37681 tatacaggtc ttcaaatgtc atatcaggtg ctttcttttt aacaccgtat aatatttgat
    37741 gtctgtactc gtcataatct ttttgagctt cttgtttgct actaaaacct cttctcgtag
    37801 tatttatttt ttcaccagtt cgtggatcgg tggcaatgtg tcctttatac atccacgcca
    37861 cttgaccgtt tttctttttg tactttgata tttttgccat gaaatcatct cctcttagtt
    37921 tacaaggagt acttaccact agatggtatc acctcccttc atttttttct tagtgtattt
    37981 tttcccaatt gatgaaatcg tttgttctag cttgtttata ttatcagtaa tttctctcac
    38041 agcttcttcg ggataagaca tgtctttcaa ttgaataggt agccattcat ctaccattct
    38101 ttctagtagt tcttctatac tgctagtgag gaaaagaatg ttattgtcaa attcttctac
    38161 aatagcattg atgaataatt cttctatttt taagatattg aaaagactaa ttttttcatt
    38221 tttaacttta ttacaagtct tgttagcaat aaattcttgc atctctggtt ctaatttgga
    38281 atatctatcc agccatgtat ctaaattaga gtgtactggt gggaactgtt gacgaagatg
    38341 ctggaacagg tttttatctg gaaatgttat accatctagt acaatgctat ctctataaag
    38401 aataattccc aaaatatatt cttctacatt gccacaaact aactcctcta tactcatatt
    38461 tcccaaatca gcaattcggg ataatcgctc tggtattggt aaataggttc ccttctccca
    38521 ttcgtagata gtttttttgt caattaattg accgttgtta atcataagac caaatgctgg
    38581 ttgtgttaat tttttactct ttcttatagc ttttattttt gttccgagta tcttttttcg
    38641 ttctaaataa tttattggtg gacgttttat catattgacc acacctccta aaattagtat
    38701 acctcgaaaa aggggtgttg taaataaatt ttacaaagat attgacacct cttttttatc
    38761 gaggtattat gtgtatgtcg atgaactggt aatcattttt tgtttgatta atcggttaat
    38821 aacaaaatac ttttattttg agcaaaaaat acgaagataa ttcttagaaa atttgttcaa
    38881 gcaatcacga aaggaggggg aaatatgtct ctacaaatca ttgatagcat tgtttctata
    38941 caagttgaac aacaagtgaa agagataatt acagtgctaa agcctatgct agaagacatt
    39001 atttctggag tcactctaga gtatatggac tatcagcagg caagtgaata tctaggtgta
    39061 tcagtaggaa cgattagaaa atatgtttct caatacgggt tacctgttat aaaaattgat
    39121 acggtcatta gatttaaaaa atctgatatt gatagtttca tggaacggta taaaggttga
    39181 gtaaacaaat aagactaaaa catatagtaa taaagagttt gtttttatta tctgtatgtt
    39241 ttagtcttta acagaatttt ataaggagga aacacaaatg gaaacatcaa tgattaatat
    39301 tccaatttat gtagataaag aaaaattatt agagaagcca gttacaaaaa aagaaaaaac
    39361 tgatgtaaga gataggtgtt tagataacta tagagaagtt acaagagatg agtttgtaga
    39421 gatactaaat tcatcacaat catttattcc aagcaagaca aagaataaag gcaatgacaa
    39481 ggagagtttt gtggaaacca gagtaattat tctggatgtc gataacactg ttaaagatga
    39541 gaaggggaag gtagtagatt tgagtaaaga tgatagtaga tacttaagca tagaaaaggt
    39601 tttatctatc aatttagttc ataattctgc gtttgcgata caaaaaagta ttcggtactc
    39661 tgaaaatcta gaaaaataca agattgtctt tctattaaaa gaagcaatta ctgactatgg
    39721 taaaatggtt gctgtttacg aatacttgaa aaagaacatc cccgggtgtg atgacaatgt
    39781 aaatgcttcg aatagaatgt tttttggagg atataagtca gatgcagtga taataattaa
    39841 tgaagacaac atgttagata ttactgagct tccgattgat tttaccatga catgttctaa
    39901 aaattgtcaa agtgattcag ggagagagta tgaactagtc aatgaaaccg attttgttaa
    39961 gttgataaaa aatgacgaca aagaagaaat gaaacagtgg tttaaagact gcctgtttga
    40021 ttcctccgac atgtctttta atgagattta tgaaagattg ttaaccattg atatgaataa
    40081 attacttaag tcaagtaaaa acttaagatg tctgtttcac aacgatcata atccctctgc
    40141 tagtattttt accagtaaaa ctgggcattc cattttttat tgtcattcca gtaattgtga
    40201 tatcagtgaa aattttattg gagttgtgat gctaatttta aagaaagaca gtcgggtaga
    40261 aacatttaat tggctattag agaacctaga cttgtatcct acacattttc gaaaattgaa
    40321 agaggaatca aaagtacttt ttgacactct tgaatcaatg aaagacaaac ttttcaggac
    40381 aataaggaat tatctagagg aaatgcgacg tgtctatgat gtcttgcttt ctgatgttaa
    40441 tttttttgat gatagtaagg aaagtgtgac ttgtattttg tcgggggaac aattagctaa
    40501 aaaaatgagt ttttactatg gtaaaactta tgacatagat aaatgtaata agttactatc
    40561 acttatgact tttataggat tgataaaaaa attagatgat gaagatgttc ctttgaagat
    40621 gctcgaatac ctaaataggt taaaggaaga gaaagcgtta ggaaacttta atcataagag
    40681 aagcaatgtc tatcgattag acgtttttaa tgcaacttct gtgattcaaa aattgaatga
    40741 agatatcctt ccacttctgg aaaaatttca ttttacatat caatattttt cttatgaatg
    40801 ggttaagata tgttttaacg aaaaagaggc taaaagagta tttcctcaaa attcccgatc
    40861 agaacttagc aaggaaaaac aattgatttt agatgaagca catgaagttt tacagggctt
    40921 atcgtggcaa ggaacatatc taatgacaga gaaagaactt actaacactg tttgtgaacg
    40981 tctacgtaat gttgggaata acagcgtgga aaatgcttta aaagaaaaca gaggctactt
    41041 tgttaaacaa ggattcactc ttgaacgagc ttctaaggat attaagagac tatacaatgt
    41101 gaaagcaagt agtagttttt tagtctacct agcaaaagga gcagggatag atgaagtaat
    41161 aaaagaattg atattatcag gagttgaatt agttcctctt tatcaattta caagtgtaac
    41221 aagagatgat ggacggagag taaggaagag aaaagaccca attgtattaa actagacagg
    41281 ttatccatag ctcttaatct tatttcatct caatacaccc accgttaaag ttcggtgggt
    41341 tatttatttt tcaatctttc aatagctgat tgcgttcatt ttcttgcctt tttaatagct
    41401 tcatatagtc aattgttcga actgcatctt cagtgctaag agaacgatag agagccaaaa
    41461 actcttcctc ataatctacg acaacattgt ctagaaaatg gcttacaggg gttcctaggg
    41521 ctctagaaag agcggttaag ctatcgaggt taggctctgt tacagaattt tcccatcttg
    41581 ctactgcctg ttgtgtagag aagatttttt cagctaattc tttttgtgtc cacccgagag
    41641 cttttcgctt ttctctaatg acctttgcat attgtttctg tttcattaat acacccccat
    41701 ctcagtatag tacaacaagt tgttgtattt gtaatggtat ttgaagtttt tttatatttt
    41761 atgagttgaa atacatcata ttgttgtata taataaaaca ggaggggaga atcatgaaaa
    41821 aagtaagtaa tctcagccga tacagaaaag aaaaaggact ttctcaaacg gagttagcaa
    41881 aaaaatgaat gttacacagc aatgtgtaag ttcgtggcaa acagggagaa cgattcctaa
    41941 accctatcaa atgaagatgc tttctgagat attaagtgta ccaatgaatg agctgttttc
    42001 tgatgtattt aataaagtaa acagttaact aataaacaat tgaatgtttt taggaggaga
    42061 agacatgtat tgtagaaaat gtggcaaaga aataacagat gaaagtaagt attgccagtt
    42121 ttgcggagca ctacaagaaa cagatgagac aactggagca gacgcagtaa agaaaagtac
    42181 tgagcagaca attaatgaaa tgaaggaaca aatcgtacat ggacaagaga cagggaagct
    42241 gtatttgatt attggttgga tttctatggt agtagcgtta gcatttattc ctgttgtatt
    42301 tggtgctgtt gctgttatta tgggctactt atatcgagat aaagatgaac aaatgggtac
    42361 gattttgatg atagcagggg tagctggagc aatctttggt gtacttttag ggatgtcaat
    42421 gtactgataa gagggataag agcaaattat tttggagata ggagataaaa agttgaataa
    42481 agtaaatatg aacaagaagt ttatcattat agttatcgtt gtcttggcta ttataggtat
    42541 cattttttca aaaatgacag gtacatctgg attagagaag tcatcgaaac cattggtaac
    42601 acagatttta caagaacagt acaacttaaa tcgatcatgt gatgatgtga cgattactca
    42661 tgaaaatgga gataacacat accgggctaa agctatatta gacaatggaa gtgctatcaa
    42721 cataaatatt gagtactatc caaagaaaga ccgtatatat gtggagatac catatgctga
    42781 ggtgttaatg ttaaat
//
LOCUS       DAZULG010000013        36364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000013 DAZULG010000000
VERSION     DAZULG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.013"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..676
                     /locus_tag="ACG9CY_000602"
     CDS             <1..676
                     /locus_tag="ACG9CY_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4937138.1"
                     /translation="NSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFE
                     QYLRYASSFGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMV
                     GIEPGLNHPVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFG
                     EKICAVHLKDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRY
                     GRSLLE"
     gene            841..1740
                     /locus_tag="ACG9CY_000603"
     CDS             841..1740
                     /locus_tag="ACG9CY_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4937139.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(1866..2735)
                     /locus_tag="ACG9CY_000604"
     CDS             complement(1866..2735)
                     /locus_tag="ACG9CY_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4937140.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            2888..4348
                     /gene="rhaB"
                     /locus_tag="ACG9CY_000605"
     CDS             2888..4348
                     /gene="rhaB"
                     /locus_tag="ACG9CY_000605"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS4937141.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            4345..5601
                     /gene="rhaA"
                     /locus_tag="ACG9CY_000606"
     CDS             4345..5601
                     /gene="rhaA"
                     /locus_tag="ACG9CY_000606"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS4937142.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            5615..6460
                     /gene="rhaD"
                     /locus_tag="ACG9CY_000607"
     CDS             5615..6460
                     /gene="rhaD"
                     /locus_tag="ACG9CY_000607"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS4937143.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            6462..6773
                     /gene="rhaM"
                     /locus_tag="ACG9CY_000608"
     CDS             6462..6773
                     /gene="rhaM"
                     /locus_tag="ACG9CY_000608"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS4937144.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            6855..8006
                     /gene="fucO"
                     /locus_tag="ACG9CY_000609"
     CDS             6855..8006
                     /gene="fucO"
                     /locus_tag="ACG9CY_000609"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS4937145.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            8133..8891
                     /locus_tag="ACG9CY_000610"
     CDS             8133..8891
                     /locus_tag="ACG9CY_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS4937146.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            8991..9635
                     /locus_tag="ACG9CY_000611"
     CDS             8991..9635
                     /locus_tag="ACG9CY_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4937147.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            9642..10649
                     /locus_tag="ACG9CY_000612"
     CDS             9642..10649
                     /locus_tag="ACG9CY_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS4937148.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            10665..11507
                     /gene="kduI"
                     /locus_tag="ACG9CY_000613"
     CDS             10665..11507
                     /gene="kduI"
                     /locus_tag="ACG9CY_000613"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS4937149.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            11527..12330
                     /locus_tag="ACG9CY_000614"
     CDS             11527..12330
                     /locus_tag="ACG9CY_000614"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS4937150.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(12579..13643)
                     /gene="fni"
                     /locus_tag="ACG9CY_000615"
     CDS             complement(12579..13643)
                     /gene="fni"
                     /locus_tag="ACG9CY_000615"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS4937151.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(13640..14725)
                     /locus_tag="ACG9CY_000616"
     CDS             complement(13640..14725)
                     /locus_tag="ACG9CY_000616"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS4937152.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(14738..15715)
                     /gene="mvaD"
                     /locus_tag="ACG9CY_000617"
     CDS             complement(14738..15715)
                     /gene="mvaD"
                     /locus_tag="ACG9CY_000617"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS4937153.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(15708..16652)
                     /gene="mvk"
                     /locus_tag="ACG9CY_000618"
     CDS             complement(15708..16652)
                     /gene="mvk"
                     /locus_tag="ACG9CY_000618"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS4937154.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            16968..17627
                     /locus_tag="ACG9CY_000619"
     CDS             16968..17627
                     /locus_tag="ACG9CY_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4937155.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            17712..18617
                     /locus_tag="ACG9CY_000620"
     CDS             17712..18617
                     /locus_tag="ACG9CY_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4937156.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(18619..19251)
                     /locus_tag="ACG9CY_000621"
     CDS             complement(18619..19251)
                     /locus_tag="ACG9CY_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4937157.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    19402..19527
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19571..20095
                     /gene="infC"
                     /locus_tag="ACG9CY_000622"
     CDS             19571..20095
                     /gene="infC"
                     /locus_tag="ACG9CY_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS4937158.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            20167..20367
                     /gene="rpmI"
                     /locus_tag="ACG9CY_000623"
     CDS             20167..20367
                     /gene="rpmI"
                     /locus_tag="ACG9CY_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS4937159.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            20420..20779
                     /gene="rplT"
                     /locus_tag="ACG9CY_000624"
     CDS             20420..20779
                     /gene="rplT"
                     /locus_tag="ACG9CY_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS4937160.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            21031..22368
                     /locus_tag="ACG9CY_000625"
     CDS             21031..22368
                     /locus_tag="ACG9CY_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS4937161.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            22416..24545
                     /locus_tag="ACG9CY_000626"
     CDS             22416..24545
                     /locus_tag="ACG9CY_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS4937162.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            24520..25209
                     /locus_tag="ACG9CY_000627"
     CDS             24520..25209
                     /locus_tag="ACG9CY_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937163.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(25617..26230)
                     /locus_tag="ACG9CY_000628"
                     /pseudo
     CDS             complement(25617..26230)
                     /locus_tag="ACG9CY_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(26366..26560)
                     /locus_tag="ACG9CY_000629"
     CDS             complement(26366..26560)
                     /locus_tag="ACG9CY_000629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937164.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(26610..27050)
                     /locus_tag="ACG9CY_000630"
     CDS             complement(26610..27050)
                     /locus_tag="ACG9CY_000630"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS4937165.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(27054..27899)
                     /locus_tag="ACG9CY_000631"
     CDS             complement(27054..27899)
                     /locus_tag="ACG9CY_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS4937166.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            28048..29466
                     /gene="pepV"
                     /locus_tag="ACG9CY_000632"
     CDS             28048..29466
                     /gene="pepV"
                     /locus_tag="ACG9CY_000632"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS4937167.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(29523..30470)
                     /locus_tag="ACG9CY_000633"
     CDS             complement(29523..30470)
                     /locus_tag="ACG9CY_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4937168.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(30693..31043)
                     /locus_tag="ACG9CY_000634"
     CDS             complement(30693..31043)
                     /locus_tag="ACG9CY_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4937169.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            31243..32322
                     /gene="pepA"
                     /locus_tag="ACG9CY_000635"
     CDS             31243..32322
                     /gene="pepA"
                     /locus_tag="ACG9CY_000635"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS4937170.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            32466..32786
                     /locus_tag="ACG9CY_000636"
     CDS             32466..32786
                     /locus_tag="ACG9CY_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4937171.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            32808..33272
                     /locus_tag="ACG9CY_000637"
     CDS             32808..33272
                     /locus_tag="ACG9CY_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4937172.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            33477..34082
                     /gene="ytpR"
                     /locus_tag="ACG9CY_000638"
     CDS             33477..34082
                     /gene="ytpR"
                     /locus_tag="ACG9CY_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS4937173.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(34121..35410)
                     /locus_tag="ACG9CY_000639"
     CDS             complement(34121..35410)
                     /locus_tag="ACG9CY_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS4937174.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            35838..36317
                     /gene="rlmH"
                     /locus_tag="ACG9CY_000640"
     CDS             35838..36317
                     /gene="rlmH"
                     /locus_tag="ACG9CY_000640"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS4937175.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT        11493 a         6688 c         7728 g        10455 t
ORIGIN      
        1 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
       61 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
      121 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
      181 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
      241 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
      301 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
      361 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
      421 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
      481 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
      541 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
      601 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
      661 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
      721 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
      781 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
      841 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
      901 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
      961 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
     1021 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
     1081 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
     1141 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
     1201 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
     1261 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
     1321 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
     1381 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
     1441 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
     1501 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
     1561 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
     1621 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
     1681 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
     1741 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc
     1801 ggttatttta tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat
     1861 atgaatcagc tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg
     1921 cggctaaagt aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat
     1981 tctgtatacc gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt
     2041 ggtggagaac cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat
     2101 tgttgttcta attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc
     2161 agtttgtaaa cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa
     2221 atcagttcat tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc
     2281 gtgccttgtt gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga
     2341 tctcctgttg aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg
     2401 acgttctgac cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga
     2461 taacgctgat attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga
     2521 ttatcattat atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt
     2581 aaatgtgctc gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata
     2641 taaaaaaata aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc
     2701 gaccatgagt ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat
     2761 aatgcaatta tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca
     2821 ttgtcaataa atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg
     2881 tggagaaatg aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat
     2941 gaaaagtacg agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa
     3001 cggctttcgg aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat
     3061 tttgattgga tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga
     3121 tacatgggca gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat
     3181 tgcttatcgg gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct
     3241 agataagttg tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt
     3301 gtttgctgaa gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta
     3361 tttagggtat gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca
     3421 gctgctgaat gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc
     3481 gaaggaatta tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga
     3541 acaattttca aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac
     3601 ggcgtcagct gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg
     3661 gacatggtcg cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc
     3721 tgaaaattat acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat
     3781 ggggatgtgg ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga
     3841 attggcagag cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc
     3901 tcgatttttg aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga
     3961 gcagccagtc cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct
     4021 ttgttacgca caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct
     4081 gcacatcgta gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc
     4141 cggaatcatt gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca
     4201 aatgctcagt acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc
     4261 gtttccatgt gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt
     4321 gaataaaatc aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa
     4381 gaaaaatatg caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc
     4441 aaaatttcta tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc
     4501 gaattgaccg gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa
     4561 gagcttagac aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac
     4621 cttcatgcta tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa
     4681 cattttgaaa aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg
     4741 actttctttt cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa
     4801 gttcgtgaat actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg
     4861 aaagaaactg ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat
     4921 ccgattgatc gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca
     4981 gaagagttag atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc
     5041 gctgaagctt atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac
     5101 aaacttgttt gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta
     5161 tcttcattag ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg
     5221 gattcagacc atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc
     5281 cgtaacgaat tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac
     5341 cgtatcgctg cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg
     5401 ctggaaccag ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg
     5461 gcttatacgg aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac
     5521 aaaaatcaag ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac
     5581 gtgttggaat taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc
     5641 gccttatgtg aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga
     5701 aagaaatggc gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga
     5761 tcctgaaaaa gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg
     5821 ctattttatt gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga
     5881 gaatcttgga atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct
     5941 ggaaaacgaa tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg
     6001 tttagaagta gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc
     6061 gatgagtttt acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg
     6121 tacggaatgc ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg
     6181 aacaaatgag attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg
     6241 gccacaacat ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga
     6301 gacagcagaa aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca
     6361 aacaatcacg gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc
     6421 aggttatttg gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt
     6481 gcatgaaagt ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc
     6541 cagaaatgcg tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc
     6601 cgagtacaag tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa
     6661 tggcaaatac atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa
     6721 atccagatgc ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag
     6781 caactgggca accggttgct tggggttctt tcgtttttat tgtgaaaaat aatacaaatt
     6841 aggaggaata gaaaatgagt aatcgaatga ttttaaatga aacatcgtat tttggaaaag
     6901 gagcgattga gtcgatcgtc cctgaatttc agcagcgagg attcggcaaa gcagctgtca
     6961 ttactgacaa aggattgatc gagcacggta tagcaacaaa agtaacagaa cttctagata
     7021 aagcagcaat cgcttatgct ttatacgacg ggatcgtacc taatccaacg atccaaaatg
     7081 taaaagatgg agtggctttt gttaaagagg cagaggcaga ctgtttgatc gcaatcggtg
     7141 gaggttcgcc aatcgatacg gcaaaagcca ttgggattat tttgacgaat ccagagtttt
     7201 cagacgtcat tagtttagaa ggtgttgctg atacgaaaaa tccatgttta cctatattag
     7261 ctataccaac gacttctgga acagcagcag aagtaacgat caactacgtc atcacagatg
     7321 aagtaaatca ccgaaaattt gtttgtgttg atcctcatga tatcccaatc gtcgcgttca
     7381 tcgacagcga tatgatgatg ggaatgccga aaaaattggc tgcatcaaca ggtatggatg
     7441 ctatgacgca tgctattgaa gggtttatca caaaaggcgc ttgggaaatg actgatatgc
     7501 tgcatttaaa agcaatcgaa atcatcggcc attctttgga agtttctgta gatggcgatc
     7561 aaaatgggcg agaaaaaatg gcattaggac agtatattgc gggaatgggc ttctcaaatg
     7621 tcggtttagg actggtacac gggatggctc acccgttaag tgcatggtac aacattcctc
     7681 atggcgtagc gtgtgcagca cttttgccaa ctgtcatgaa atataacaaa gaatatactg
     7741 gagaaaagta ccgtgagatc gctcttgttc tagggattaa aggagcagct gaaatgtctt
     7801 tagaagatgt aagagaagcg gcttgtggtg agatcgatcg gttgagcaaa gcggttggaa
     7861 tcccagaaac catttctgaa ttaggtgtaa aagaagcaga tattccagca attgcagaag
     7921 atgctttgcg agatgtatgc acaccaggaa atccaagaga aacgacagta gaagaaatca
     7981 ttgctttata tcaaagtttg atgtaaaaac gaagcggatc ttcaacatta aattgcctgt
     8041 aaagagaaat ggtagtttat atctggatat ctcttttaaa ttgatggtat ttccgctata
     8101 atttttctta taaagaaata ggagaatatt atatggaaaa taaaaagcct tatggaactg
     8161 tattgatcaa agcctctaaa atactagact atttagcgga aaaccaagat gtctctttgc
     8221 agacgatcgc aaagggtgtg ggaatgacct cttctacagt cttgaaaata ctagatactt
     8281 tgttgatgat cggctacgta aataaaaatt cagagaaaaa ctatcgttta ggtgcaaagc
     8341 ttattcgcta cgcgaataaa aacattgaac aaatcgattt ggtagagttg actttaccgt
     8401 ttttagaaca gctgcagcaa aagatcgatg agactatcca cttaggggta ttggacaaca
     8461 atgagatctt gtacgtgaac aaactagatc caaaacatca aacgatccgc atgtcttcaa
     8521 aaatcggaat cacccgacca ttatacaatt ctgcgatggg caaagccgta ttagcagaat
     8581 tcagtgaaga gcagtacact gattatcttg aaaagcatcc gttgatccct tacacagaat
     8641 atacgatcac caatgcatta cggttgaaaa aggaaatcca aacggtaaaa gaaacccaca
     8701 tcgcatttga cgatgaagaa atcgaacgtg atattttttg tataggagcc tcaatcgtga
     8761 aaaacgaaca aatcattggt gcatttagtg taagtatgcc gaaatatcgt ttaacagatg
     8821 agacaaaaga acaaataatc agtgccctca agcaaacaaa aagcgaaatc gagcaaagaa
     8881 ttcaaaaata atgaaaaaag cgtttgcaat ttttcttttc tgccgtataa tatgggtata
     8941 gatttccaag ttataaaata aaatttctaa ataggaaata ggtggagaag atgaaaaaaa
     9001 ttgatatttt atctcgattg aaaaacgccg gagtgattgc ggtggtacgc ggaaaaagca
     9061 aagaagaagc tttgaatgct tgccacgcaa tcatcaaagg tggtctaact ggtattgagt
     9121 tgacttttac agtccctcaa gcagatcaag taatcaaaga acttttgtca ttctacaaag
     9181 accaaccaga aatagtgatt ggcgcaggca cagtattaga cgctgtcaca gctcgcttgg
     9241 ctatcttagc tggcgcagag tatattgtta gcccttcctt tgatcaagaa acggcagaga
     9301 tgtgcaatct gtatcaaatt ccttacttac caggatgtat gactattacc gagatcaaaa
     9361 cagcgctgaa aagcggggta gatatcgtca aactatttcc tggaagcgct tatggaccaa
     9421 gtatcatttc tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca actggcggtg
     9481 tgagtctgga caatatgaaa gagtggttcg atgctggtgt cgtaacagtc ggagtaggtg
     9541 ggaacctttt agcacccgca gctacaggag attttgacaa agtaacggaa gtcgcgcaac
     9601 agtatgcagc aaaaatgaaa gaaatcaaga ggtagctgct tatgggaaaa gtagtgacac
     9661 tcggagaaat catgttgcga ttgtccacag agcagggaac ccgtatcgat tcaagtgaac
     9721 gattcgctgc ccattatggc ggaggggaag caaacgtagc aatttcttta gctaattacg
     9781 gacatgaagc gatttttgct acaaaagtac ctgataacgc attgggaaaa gcagtcaaaa
     9841 agcacttgca atgttatgga gtagatacga aacaggtatt atctggtgga tcaagactag
     9901 gtacttatta tatggaaact ggtagtggcg aacgcgccgc ctcagtgatc tatgatcgtg
     9961 ccggatcaag ttttgctgaa atggatcatt tggaatggtc gctagaggag ttattccaag
    10021 gaatagatat atttcatgtt tcaggaatca ccccagcttt gtctaaaaaa tggcaaaaaa
    10081 tgacgaaaca ggtgatagaa gctgcaaaaa aagctggctg cctgatcagc ttcgatatca
    10141 actatcgtgg aaaattatgg acacagcaag aagccagcga agtaatccat cagcttttac
    10201 ctttagtaga tatctgctca gctggaaaaa tggatgctct ttatttgtta ggtatcgaga
    10261 aagcgccaga agcagaagaa caacctctaa tttattatta tcaaaaaatg caggaaaagt
    10321 tcccgaatat tcaggtgttt tattcgacca agcgtaccgt tcattcagca agtgaaaatg
    10381 aattgaaggg gacattgtgg atgaaccatg aatatgtgga atcccaagtc catagaatca
    10441 ccccaatcgt ggatcgagta ggtggcggag atgcttttgc aggtggcgtg ctccatggga
    10501 ttttatccaa tatgtctccg caagaaatca ttgattttgc tacggcagct tctgccttga
    10561 agcatacgat ccatggggac tgcaaccaat ttagtcaaga agaagtcgca caatttttag
    10621 catgcggctc tggaaaaatc attcgataga aggagtagaa gaaaatgcaa gaaatggaaa
    10681 cacgttatac acacagtcca gaagatatcc gtcattattc aacggaacaa ttgagaaaag
    10741 aatttttagt agaaaaggta tttgaaccag ggaaaatcag tttgacttat acgcacaacg
    10801 atcgaatgat ctttggtgga gtgacaccta ctgaaaaaga attagaaatc acattagaca
    10861 aagaactagg tgttgattac ttcttagagc gtcgtgaact aggtgtgatc aatatcggtg
    10921 gaccaggatt catcgagatc gatgggcaaa aagaagaaat gaaaaaacaa gacggatact
    10981 atatcggaaa agaaacacga catgttgtat tttcttctca agatgctgaa aacccagcta
    11041 aattctatat tagttctgtt ccagctcatc ataaatatcc aaatgtaaaa atcagtatcg
    11101 accaagtaaa accaatggag actggtgaag gcctgacact gaatgaacgt aaaatctacc
    11161 aatacatcca tccaaatgta tgcgaaagct gtcaattaca aatgggttat acgattttag
    11221 aaccaggaag cgcttggaat acgatgcctt gtcatacaca tgaacgtcgt atggaagctt
    11281 atgtctactt tgattatgcc aatgaagata cacgtgtgtt ccacatgatg ggcaaaccag
    11341 atgaaacaaa acatttagtt gtagacaatg aacaagccat tatttcgcca agctggtcga
    11401 tccattcagg tgtaggtaca agcaattact cattcatctg ggcaatgtgc ggagaaaata
    11461 tcacctatac agacatggat atggtgcaaa tggatcaatt aaaataagca taagggagag
    11521 aaaatgatgg aatttagtat ggatatgttt cgtttagatg gaaaagtagc attagtcacc
    11581 ggtgctgtct atggtattgg atttgagatt gcacgctctt tagctacagc aggagcaaaa
    11641 atcgtattta ataacttgaa tcaagaatca gttgatgaag gtatagcgca ttataaagaa
    11701 gcaggcatcg atgcaaaagg ttatgtctgc gatgtgaccg atgaagaagc ggttcaagcg
    11761 atggttcaac agatcaaaga agatgtaggt tcagttgata ttctagtcaa caatgcaggg
    11821 atcatcaaac ggacaccgat gatcgaaatg gatgcggcag acttccgcca agtaatcgac
    11881 gtggatttaa atgccccatt catcgtttca aaagcagtta ttccggatat gatcgaaaaa
    11941 ggcggcggta aaatcatcaa tatctgctcc atgatgagtg aattaggacg cgaaacggtc
    12001 agtgcttatg ctgcggcaaa aggcggatta aaaatgctga cgaaaaacat cgcttctgaa
    12061 tacggtcaat acaatatcca atgtaatgga attggaccag gttatattgc tacaccgcaa
    12121 actgctccat tacgcgaaac acaagaaaac ggtgaacgtc atccattcga tcaatttatc
    12181 gttggacgta cgccagcagc acgctggggc gagccaatcg atttggctgg accatctgtc
    12241 ttcttggctt ctagcgcatc tgatttcgtt aacggccatg tcttgtatgt ggacggaggc
    12301 atacttgcct atatcggaaa acaaccttaa aagagcttta gtacactgtt tagtggagag
    12361 gttgtgaaat cagcggattg aacgaatacc gaaggacagc tgattaaaca tcatttattc
    12421 gaaaaaagag gctgggacag aagcatttaa ctccaagaaa taagaaggaa ttcacgaaaa
    12481 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ttacttccgc
    12541 cgtttgtacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
    12601 tctgtgcgtc ttccatactt cactaagtcg atccctcttg cttcgcacca atccttcact
    12661 ggtccggaaa agatcagaga ttgttggtgt agggccgcgg tattcgttgc gccgaccata
    12721 gtataaagca gacgaagctc ttcttgccat tgcttcatca gtataatagt ttcttctacg
    12781 ccgtgattca tcagatgcgt cagtacggtt cctgaagtac ccacggcatt tgcgccgaga
    12841 caaagggctt taaatatgtc ataggcattg cggatacctc cggaggctag gatttccata
    12901 gaagtgtctg cttcatttgc ttcaagcaat gaagagactg tcgattgccc ccagtcagct
    12961 aaataactca gttcccgctt ggagcgacgg gcgttttcga tctgggtgaa gcttgttccg
    13021 cttcgaccgc tgatatcaac tgtatgaact cctaaagcag ctagatcatt gagcgtttct
    13081 cttgtcatgc caaaaccgac ttcttttacg atgaggggga cgtcaatggc tgtttgagtt
    13141 tcttgaataa gtactttcca attggtaaaa tcacggtctc cctcaggcat aaccaactct
    13201 tgaggtgcat tcaaatgaat ctgtaaggca tctgcgtgaa acaatcggat agcttctttg
    13261 gctctttcga cagaagtccc tgcaccgatg ttggcaatga ttttcccgtg aggatattct
    13321 tgtcgcataa tcgtataagt atcggctaaa gaagggactt tcaaggcagc gctgactgaa
    13381 ccagtggcaa tcatcaaatc tgcttctctt gcgacgattg caagatcttg gttgattttt
    13441 tttgtttttt cgcttccgcc agtcatcgca ttgatataaa atgggctgct tagtgtaaac
    13501 ccaactgcct gtgtacttag atcgacatca gaaactgcta tttgcggcaa agggttatgg
    13561 atgatccgta cgaagtcaaa ttcgtttttt tgtttatcat gaaaggcttt agctagtgaa
    13621 acatgttcgt cttttcgatt catcggtttt cctttctttg atcgctatag acatgtaacg
    13681 gcagtggagt gatttctgct ttttcccatg cactcattaa aggaagaatg ccagatttct
    13741 ggtcaacaat tacgattccg caatcgcccc cacctgcacc agaagatttt gcggctcctt
    13801 catactgttc agctaaatta cacaatttgt tcaaagcagg cgtttcgatg acgaccccag
    13861 tgattgcaga taaatcatgc agtaattgtc ggttttttcg aatcatctgt tggatcaacg
    13921 ttacattatt ttctttaaac cctttgatca tttcgttgac acattctgta ctatttttta
    13981 agaaaagctg ataagccacc attttatctt ctctcgaacg gtgaacttga tcgaccaaat
    14041 cagaagtaga ggcagggcta cccgtccaac caatcaataa acgtaaatct tcaggagcaa
    14101 tcaatggctc aatggataga cctggccaat ccagggcaag taactcactg atagaatgct
    14161 gagtttcttg ttcttggagc caaggatggt cgaaggttga gaaagcgatc cagccaccat
    14221 aacagctagc agcgatgtcg ccgcaggaac cattatcttg aactgctaaa ttggctagtg
    14281 ctgcaatctt gaaaatctcc aactgagata aattcaacgc gtaaaaaaca ttcaaggctt
    14341 tgaccgttgc aacggtcacg gcaccgctag aacccaatcc atatttccgg ccatttgaac
    14401 tgtctaactc acttgttact ttcagatcat aaaaggataa aagaatgttt ttttcttgtg
    14461 catacttttc agtcaagcga atagcagcaa ggatataatg aaaaggattt tcccgaatat
    14521 ctaaaactaa ttctccgttg cgtcttgtcc aacgtacagg catcccacta tattgtgcag
    14581 attggatact tccgactttt cgtgcagatt ctacagtgac tgtcacgaat tgatcgactg
    14641 cagcgataac tgctggatgg cctgtttcaa caactgcata ttctccggca atatagagtt
    14701 tgcctggtgc agatacttca atcataaaaa tctcccttta ttcaataatc gcaattcctg
    14761 gaccagcatg tgcagtaatt acttgttctt tgctaaataa tttggtaaat gtttcttgta
    14821 cctcagataa atggctgttt tccactaaaa ctttgacatt agggccagca tccatcgtaa
    14881 agtagcatgg aatgccttgc tttctcaatt gtcgcaccgc atccatcgct tttaagctgt
    14941 caggagacca gtaagtgaat ggaggctggg cagctaaagt cgttccatgc atttttagtc
    15001 cgtttctttc catcgtttct ccgagaagtt ggaaatcttt tgttttgatt gcttgtttca
    15061 attgatatag atccccttca acggaatcta accagccttg ataaaaactg gatgtttcga
    15121 ctgtccgacg catcccattt ctgctggaca cttctttttt ctggtcgttg atcaaaacga
    15181 aaaccattgc taaatcgtct tcgaaagaat cggaagggac tggcttagcg taagaactta
    15241 agtcgtcatg gcctttttcc cattcgacga aaccaccgaa aatacttcgg caagctgaac
    15301 cagacccgcg tcgagcaaat cttgaaagag agagatcatc taatccaagc ttaagagcag
    15361 tattgcaagc gcctgctaaa gcagctagcc cgctggcaga ggaggctaat ccagctgcgg
    15421 taggaacgaa attctgactg attactgaag cttttagtga gaggccggct tctttacgaa
    15481 aaaggtcaag aaactgactg actttttttg ttgctttttc gtcttgcaag gtgccatcta
    15541 gataaaattc atctaccata tagctatcag aaaagatgac ttctgtctct gtataaaatg
    15601 catccaatgt taacgaaagg ctgttgttca ttggaaggat gagttcttca tttttcttac
    15661 cccaatattt gattagagca atgttggtat atgcgcgtgc tttgccttta aacataggta
    15721 tgtactccta atccttgtat ccaagtttca gcagcacccg catcttcaag tgcttgactg
    15781 atttcttggg cttttgtttt tgtttgtgct aatgcaatca tacagccacc gcgtccaccg
    15841 ccagttaatt tggcacccaa tgccccggta tcttttgctg tttggatcaa tagattaaga
    15901 aaatcattgc tgattgtcag cttttccaga agtgattgtg attcatccat tgtctgtgcc
    15961 aacgtttctg gtgaattttc aatgattgct tgttttgctt gcttcgtcaa gtatcctaat
    16021 ttttggatca tttgtccagt ttcatgaggc tgggtttcaa agaggtgagc gacatctttg
    16081 acggcttctc ttgtttgtcc tttgattcct gtatcagcga caatcaaaaa agcatcgatg
    16141 ttcaaagaaa agtagtcgaa aggatgccct tttgtaaaat aaatcggctg caagctgcta
    16201 gtagcggctg catcgattcc actaggatta ccgtgggcga ttttttccga aagctggaca
    16261 ttttctaata gtatttcacg agacaaagga aatgctaagt aatcataaaa agcacgagtg
    16321 actgctgtgg cgactgcagc gcttgatccc attccacgtt cagcaggaat cgtgctttca
    16381 atggtcagtt gcaagttttt ttggatgtca tgctgttttt ttaagtgaat gaataatttt
    16441 ttgatatttt ttaatgcatg aggagcttcg gctaaatttc ctgaatagta ggaagaaacc
    16501 agttgatcgt ccattgcatc cagctcttca aggaatgcgg tgacttttgt tgcatagaaa
    16561 ggaaaggcaa tcgctggttc tccataaaca accgcgtgct cgcccatcaa tatgatcttt
    16621 ccgcttgatt ctccttggcc atagtttgcc ataatctaac tccttctcac gtaaaagtga
    16681 tttttaagca aaaaataaag cgcccaaaac gagttgcgct gcttcacgaa tttttttgtc
    16741 cactaagggt ctcaaaaaac attcctcctt tattttacta aaaccatctc agaagtaaaa
    16801 ctaagaatac gttttctaaa gaaaaaacac ttttttctta agaatttttt gacttttttt
    16861 cttctttgac tagaaaaaaa tacaattgac actactagat attgggggta tatttaaaaa
    16921 ggttggagtt aaacacaata tttagtgatg aagtgagctg gaggtaaatg agcgtgatcg
    16981 ttttagcagg tacaattggt gccggaaaat caagtttaac agaaatgatg gctgaacact
    17041 ttgatagtca ggcattttat gaatcgattg atgataatga agtcttgccc ttattttatg
    17101 caaaccctga acagtatgct tttttgctgc aaatttattt tttgaataaa cgttttgcaa
    17161 gcatcaaaca agcaatgaaa gatgacaata atgtattgga ccgttcgatt tatgaagatt
    17221 cactgctttt ccatctaaat gcagatctag gaagagcaac agaaacagaa gttcgtgtgt
    17281 atgatgaact gctggaaaac atgatggaag aacttcccta tgctgcccat aaaaaacatc
    17341 cggatttatt agttcatatt cgtgtatctt ttgatacgat gttggagcgt atcgaaaaac
    17401 gtggtcgttc gtatgaacaa ttgtcctttg atccaagtct atacgattat tacaaagaat
    17461 tgaacagacg ttacgatcaa tggtatgaag aatacaaaga aagtccaaaa atccaaatcg
    17521 atggagaccg ctataatttt gtggaagatc ctcaagcaaa agaagaagtc ttgaaaatga
    17581 tcgaagaaaa attagcagag attcgccaaa ccaaagtagc catttgattt acagttgctc
    17641 aaataaaggg taaaccttta tttgagcgac ttttttcttt atactggaaa caaatgtcag
    17701 gaggatagag tatgcgggaa aaacactatt ttaatcaaga ccgtaatttt tggatcaatg
    17761 tacagcaaaa cgatgtgcta gctgttaaaa acttaaaaga agaatatggc attagtgacg
    17821 agatgctgac ttattctcta gataaaaatg aacgggcgcg cgtagagtac gattcttttg
    17881 atgaagcgtt actgcttgta tcaaatgttc cgcaccagca aaaagtcgat aatcattatg
    17941 aaactagtcc tatcgctttt attttaaaag aagacggttt gtttacattt accacgccga
    18001 ataccgagta cgtgattcga ctaattcgat cgcttttgga acgaatgcca gacatgagtg
    18061 tatacagttt attgtttcgt acattgtttt tgatttctga ttcctttttt cctttaatcg
    18121 aggaagtgaa tagtgaacgt cagcggctta atctgaaatt aagagaaaaa acaacgaaca
    18181 agaatctgct gcaactttca gatttagaga tcggtttggt ttacttagtt acaggaacaa
    18241 aacaaaatgt agtactttta gaacaaatca aggcattggc tatttatcgc aagttgtcag
    18301 aaaaggaaaa agaagagttg gacgatgcgc tgatcgaagc acgacaagca gttgaaatga
    18361 cgaacttagc ttcgcagatt ttagaccagc tctctggcac ttataataat ttgctgaata
    18421 acaacttgaa tgacacgatg aaatttttga cggtctggtc gctgatcttg accgttccaa
    18481 ctattgtgac aggattcttc ggaatgaatc tccagttacc ctttacccat tccgttttcg
    18541 gatggggaat cgccttgatc atcagtttgg tcttatctat ttggatgctt attgcactgt
    18601 ggcggcggat tcgttgatct agtctttgat ctgcaaacca agggcttggg cgatcacaag
    18661 tgcttgctct tggttgacga ttagtcccgc caaagaatct tgagataaag cgattgctgt
    18721 gaagaaatta gtcgtaaagt ctaatccctt cagagaagtt cgaaaccagt tcgaaccggt
    18781 caactgattg tcagacaaaa aaaggttttt ccaagtgacc tcataaaatt cgctgtcttc
    18841 taattgacaa tgatcgaagg agacagcttt caagtttgta ctactaaagg aagccatcga
    18901 agcaatacaa tcagtgaaag tacagtctcg aagataactt tcggcaaaat tcgtacctgt
    18961 taatttacat tgatgaaaca cgacttggtg gaaactgcca ccaatccact ccagattgga
    19021 aaaatcacat ttttcaaaaa tgacattact gcattcaaaa tgacgtaaaa ctgttttttg
    19081 catcgtaagt ttttggatat gactctctct taataccata tttttggcat cgagatagct
    19141 ttggtcaccc ccgttgatca gaagaccaga gaagatctcc tcatcttcta aataaaactc
    19201 tccctttgta agaagaggaa gagaaggtgg aataggttcg atttttttca tgtgggttca
    19261 cctcgttttt ctttagtata gcaaagaagc gggaatcact gctagaaagt ctgctaatct
    19321 aatcattttc agaaaaggaa gacaaactgt atgaaaaaca ttgacaaaaa gattagtttt
    19381 tgctatacta tgatggttga gaaataaaag cagaagcacc cgcttctcgc cttagttgat
    19441 aatgtcactg ggctagatag ttttcactgg ttctattagg tgaagtacgc gcgggttctg
    19501 taagagaact cgttttttta ttgccgttta ttcggtaagc ttttctttct ctcataatat
    19561 ttggaggtga atgaccatag caaaggatat gatggttaac gacggcattc gtgcacgaga
    19621 gttacgattg atcggttcag atggtgagca attaggagtt aagacaaaag cagaagcatt
    19681 gaatattgca gaacaagcaa acttggatct tgtgttagtt gcccctggtg cgaaaccacc
    19741 agttgcgcga atcatggact acggtaaata ccgtttcgaa caacaaaaga aagaccgcga
    19801 agcgcgtaaa aaacaaaaag tgatcaatgt aaaagaagtt cgcttaagtc ctacgatcga
    19861 cttgaatgac ttcaatacaa aacttcgtaa tgcacgtaag ttcttggaaa agggcgataa
    19921 agtgaaagct tctatccgtt tcaaaggccg tgccattacc cataaagaaa ttggtcaaaa
    19981 agtcttgaat cgcttagctg aagaaactgc ggatattgca acagtggaac aaaaagcgaa
    20041 aatggacgga cgcagcatgt tcttgacgct ggcaccgaaa aacgacagta agtaatttga
    20101 atgactaact ggctgttgga cagccgatag tcggataacg aaagattttg aggaggaaat
    20161 atagtcatgc caaaacaaaa aacacaccgc ggatcagcaa aacgtttcaa acgtactggt
    20221 aaaggcggat tgaaacgctt ccgcgcgttt acaagtcacc gtttccacgg caaaacaaaa
    20281 aaacaacgcc gtcaattgcg taaagcaagc atggtttcta gtggcgactt caaacgtatt
    20341 cgccaacaac tagcaaaaat gaaataagaa gtacaaataa cagaatctga agatttattt
    20401 ttattaggag gaattataca tggcacgtgt taaaggtgga acagtaactc gtaaacgtcg
    20461 taaaagagtg ctaaaattag caaaaggtta ctacggttca aaacacactt tatttaaaac
    20521 agcaaaagaa caagtaatga attcatacaa ctacgcatat cgcgatcgtc gtcaaaagaa
    20581 acgcgacttc cgtaaattgt ggattgctcg tatcaacgca gcagctcgta tgaatggctt
    20641 gagctactca aaattaatgc acggtttgaa agttgctgaa atcgacatca accgtaaaat
    20701 gttagctgac ttggctgtta acgatgcagc agcatttact gcattggctg accaagcaaa
    20761 agaagctttg gctaaataag ctaaacaatt ataaagagga aagaagataa cgaaaagtta
    20821 tgttctttcc tcttttttta tttacaatcc tccatcaaat gaaacaggac aataacgtat
    20881 aagtctaaaa tagcataatt aaggtttttt aattgaaaac tgaacattta tggtcaccct
    20941 ttaatgaaag cgctaactac ataagttaga atgagtttga ctgatatatt agtttgctta
    21001 tcagaaaaac aaaatggacg gaggaataac atgagtgata ttattattgg tattttgtta
    21061 gttatctcat tctttttcat ggtctggtac tgtgtaaaag gctacaactt gatggtcggc
    21121 tttgccatca tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca
    21181 aacccggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg
    21241 ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt
    21301 gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg
    21361 gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg
    21421 atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccaat cttgatgtca
    21481 ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc
    21541 tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg
    21601 gctgaaagct atacttataa tgactatttc caaattggta tgattgggat gattgtaagc
    21661 ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg
    21721 gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta
    21781 ttaccagttt taggggtcgt tcttttagat cttccaatca tcttaggttt tattctagca
    21841 ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt
    21901 cagtttgcga aattatttac agatggcgca gtagatgttg caccaatggt tggtttcttg
    21961 atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt
    22021 tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca
    22081 ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct
    22141 gtcgtattag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatc
    22201 acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt
    22261 tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc
    22321 ggagcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt
    22381 cgataaaaac gattagatga atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa
    22441 ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca
    22501 tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg
    22561 ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat gttgtgtttg
    22621 ttgcacattc taccacacaa gcgactaatg cactgattga aggagacgta gcaaaagtcg
    22681 gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat
    22741 tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg
    22801 tcaaacactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag
    22861 cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg
    22921 tttatgaagc agcatcggta aaatcgatcc ctacaacgat ggcttctgac atcacaaaat
    22981 tatatggatt gacaagaaga actcggacag cagcaatcaa tgcatctatt ttaccaaaaa
    23041 tgctagatac ggcaacctct acagaagatt ccgtacgaga agcaggggtc aatgtttctt
    23101 tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg
    23161 ttttaaccat gctttccggt ccagcagcat cagtcatggg ctctttgatg tacttgagag
    23221 cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa
    23281 atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat
    23341 tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa
    23401 tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg
    23461 agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg
    23521 ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg
    23581 cagccaatgt ccttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg
    23641 cacgaaaagc catccaagca ttagctgatt attgtcatac gacggttgaa gacatcgcag
    23701 aacagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca
    23761 agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat
    23821 tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag
    23881 tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc
    23941 cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg
    24001 aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca cagacttcaa
    24061 aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga
    24121 tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag
    24181 tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa
    24241 atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg
    24301 cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag
    24361 atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag
    24421 caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg
    24481 aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa
    24541 cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc
    24601 taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt
    24661 atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct
    24721 tttgccgatg taggagaaat ttgtgaacaa atggctgttc caatcgtttc ctgtgaatca
    24781 gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg gattcgattg
    24841 atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca
    24901 gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga
    24961 atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca
    25021 aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc
    25081 gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc
    25141 caagggatct tagaagaaat aaaaaaaata gaaaaaagat gtacggagta tgatttagat
    25201 tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaacgat
    25261 gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga gaagctgttt
    25321 tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc ttggaaaacg
    25381 gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa gttaaaaaca taagaagcta
    25441 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa
    25501 acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa
    25561 ctattttgcc gtttctttct ctaatactca ggtcaaaacg ctgattccaa aacctcttat
    25621 ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct
    25681 tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta
    25741 tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat
    25801 tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata actttctagc
    25861 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
    25921 aaatcaaaaa aagctttttt attttgagcg attactgcct gatcggtttt tccaactttt
    25981 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    26041 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    26101 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    26161 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    26221 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    26281 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    26341 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttgtatc ggtttgacac
    26401 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    26461 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    26521 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    26581 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
    26641 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    26701 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    26761 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
    26821 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    26881 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    26941 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    27001 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    27061 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
    27121 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    27181 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    27241 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
    27301 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    27361 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    27421 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    27481 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
    27541 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    27601 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    27661 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    27721 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
    27781 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    27841 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    27901 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    27961 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    28021 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    28081 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    28141 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    28201 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    28261 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    28321 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    28381 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    28441 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    28501 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    28561 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    28621 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    28681 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    28741 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    28801 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    28861 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    28921 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    28981 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    29041 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    29101 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    29161 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    29221 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    29281 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    29341 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    29401 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    29461 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
    29521 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
    29581 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
    29641 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
    29701 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
    29761 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
    29821 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
    29881 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
    29941 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
    30001 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
    30061 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
    30121 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
    30181 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
    30241 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
    30301 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
    30361 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
    30421 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
    30481 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
    30541 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
    30601 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
    30661 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
    30721 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
    30781 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    30841 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    30901 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    30961 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    31021 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    31081 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    31141 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    31201 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    31261 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    31321 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    31381 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    31441 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    31501 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    31561 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    31621 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    31681 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    31741 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    31801 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    31861 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    31921 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    31981 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    32041 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    32101 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    32161 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    32221 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    32281 ttggataaat caacagtgaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    32341 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    32401 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    32461 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    32521 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    32581 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    32641 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    32701 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    32761 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    32821 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    32881 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    32941 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    33001 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    33061 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    33121 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    33181 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    33241 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    33301 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    33361 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    33421 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    33481 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    33541 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    33601 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    33661 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    33721 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    33781 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    33841 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    33901 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    33961 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    34021 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    34081 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    34141 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    34201 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    34261 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    34321 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    34381 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    34441 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    34501 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    34561 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    34621 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    34681 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    34741 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    34801 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    34861 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    34921 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    34981 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    35041 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    35101 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    35161 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    35221 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    35281 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    35341 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    35401 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    35461 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    35521 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    35581 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    35641 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    35701 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    35761 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    35821 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    35881 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    35941 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    36001 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    36061 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    36121 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    36181 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    36241 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    36301 ctccgtatca taaataatga tatttaagat tgaatgctcc tgaacactaa gttagctttt
    36361 catt
//
LOCUS       DAZULG010000014        34510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000014 DAZULG010000000
VERSION     DAZULG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.014"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG9CY_000641"
     CDS             212..604
                     /locus_tag="ACG9CY_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4937176.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG9CY_000642"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG9CY_000642"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS4937177.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG9CY_000643"
     CDS             2698..2943
                     /locus_tag="ACG9CY_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937178.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG9CY_000644"
     CDS             3165..4775
                     /locus_tag="ACG9CY_000644"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS4937179.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG9CY_000645"
     CDS             complement(4752..4964)
                     /locus_tag="ACG9CY_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937180.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG9CY_000646"
     CDS             5067..5936
                     /locus_tag="ACG9CY_000646"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS4937181.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG9CY_000647"
     CDS             6101..7372
                     /locus_tag="ACG9CY_000647"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4937182.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG9CY_000648"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG9CY_000648"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS4937183.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG9CY_000649"
     CDS             8763..9026
                     /locus_tag="ACG9CY_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS4937184.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG9CY_000650"
     CDS             9471..9920
                     /locus_tag="ACG9CY_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4937185.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG9CY_000651"
     CDS             9977..11395
                     /locus_tag="ACG9CY_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4937186.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG9CY_000652"
     CDS             11395..12255
                     /locus_tag="ACG9CY_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4937187.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG9CY_000653"
     CDS             12429..13202
                     /locus_tag="ACG9CY_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4937188.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG9CY_000654"
     CDS             13349..13714
                     /locus_tag="ACG9CY_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS4937189.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG9CY_000655"
     CDS             complement(13862..15070)
                     /locus_tag="ACG9CY_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4937190.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG9CY_000656"
     CDS             complement(15089..15367)
                     /locus_tag="ACG9CY_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937191.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG9CY_000657"
     CDS             15560..16216
                     /locus_tag="ACG9CY_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4937192.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG9CY_000658"
     CDS             complement(16658..17455)
                     /locus_tag="ACG9CY_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS4937193.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG9CY_000659"
     CDS             complement(17452..18636)
                     /locus_tag="ACG9CY_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS4937194.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG9CY_000660"
     CDS             complement(18661..19908)
                     /locus_tag="ACG9CY_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4937195.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG9CY_000661"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG9CY_000661"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS4937196.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG9CY_000662"
     CDS             21888..22202
                     /locus_tag="ACG9CY_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS4937197.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG9CY_000663"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG9CY_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS4937198.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG9CY_000664"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG9CY_000664"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4937199.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG9CY_000665"
     CDS             23546..23875
                     /locus_tag="ACG9CY_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS4937200.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG9CY_000666"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG9CY_000666"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS4937201.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG9CY_000667"
     CDS             24863..25687
                     /locus_tag="ACG9CY_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS4937202.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG9CY_000668"
     CDS             25680..26027
                     /locus_tag="ACG9CY_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS4937203.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG9CY_000669"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG9CY_000669"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS4937204.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG9CY_000670"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG9CY_000670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS4937205.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG9CY_000671"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG9CY_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS4937206.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG9CY_000672"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG9CY_000672"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS4937207.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG9CY_000673"
     CDS             31684..32451
                     /locus_tag="ACG9CY_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937208.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG9CY_000674"
     CDS             32441..34483
                     /locus_tag="ACG9CY_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4937209.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11095 a         6120 c         7248 g        10047 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat
//
LOCUS       DAZULG010000015        33943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000015 DAZULG010000000
VERSION     DAZULG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.015"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1259)
                     /locus_tag="ACG9CY_000675"
     CDS             complement(27..1259)
                     /locus_tag="ACG9CY_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4937210.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="ACG9CY_000676"
     CDS             1514..2083
                     /locus_tag="ACG9CY_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4937211.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="ACG9CY_000677"
     CDS             complement(2261..2701)
                     /locus_tag="ACG9CY_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4937212.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="ACG9CY_000678"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="ACG9CY_000678"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4937213.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="ACG9CY_000679"
     CDS             3655..4578
                     /locus_tag="ACG9CY_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS4937214.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4803..5042)
                     /locus_tag="ACG9CY_000680"
     CDS             complement(4803..5042)
                     /locus_tag="ACG9CY_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4937215.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5047..5466)
                     /locus_tag="ACG9CY_000681"
     CDS             complement(5047..5466)
                     /locus_tag="ACG9CY_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4937216.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACG9CY_000682"
                     /gene_synonym="mefH"
     CDS             complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACG9CY_000682"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFS4937217.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7038..7673)
                     /locus_tag="ACG9CY_000683"
     CDS             complement(7038..7673)
                     /locus_tag="ACG9CY_000683"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4937218.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7787..7879)
                     /locus_tag="ACG9CY_000684"
                     /pseudo
     CDS             complement(7787..7879)
                     /locus_tag="ACG9CY_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8015..8650)
                     /locus_tag="ACG9CY_000685"
     CDS             complement(8015..8650)
                     /locus_tag="ACG9CY_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4937219.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9162..10304
                     /locus_tag="ACG9CY_000686"
     CDS             9162..10304
                     /locus_tag="ACG9CY_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4937220.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10297..10503
                     /locus_tag="ACG9CY_000687"
     CDS             10297..10503
                     /locus_tag="ACG9CY_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4937221.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10582..11775
                     /locus_tag="ACG9CY_000688"
     CDS             10582..11775
                     /locus_tag="ACG9CY_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4937222.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11980..13119
                     /locus_tag="ACG9CY_000689"
     CDS             11980..13119
                     /locus_tag="ACG9CY_000689"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS4937223.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13112..13912
                     /locus_tag="ACG9CY_000690"
     CDS             13112..13912
                     /locus_tag="ACG9CY_000690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4937224.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13912..14739
                     /locus_tag="ACG9CY_000691"
     CDS             13912..14739
                     /locus_tag="ACG9CY_000691"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4937225.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14744..15451
                     /locus_tag="ACG9CY_000692"
     CDS             14744..15451
                     /locus_tag="ACG9CY_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4937226.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15551..16417
                     /gene="rfbA"
                     /locus_tag="ACG9CY_000693"
     CDS             15551..16417
                     /gene="rfbA"
                     /locus_tag="ACG9CY_000693"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4937227.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16431..17003
                     /gene="rfbC"
                     /locus_tag="ACG9CY_000694"
     CDS             16431..17003
                     /gene="rfbC"
                     /locus_tag="ACG9CY_000694"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS4937228.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17025..18053
                     /gene="rfbB"
                     /locus_tag="ACG9CY_000695"
     CDS             17025..18053
                     /gene="rfbB"
                     /locus_tag="ACG9CY_000695"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS4937229.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18151..19002
                     /gene="rfbD"
                     /locus_tag="ACG9CY_000696"
     CDS             18151..19002
                     /gene="rfbD"
                     /locus_tag="ACG9CY_000696"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS4937230.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19036..21069
                     /locus_tag="ACG9CY_000697"
     CDS             19036..21069
                     /locus_tag="ACG9CY_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937231.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21113..22393
                     /locus_tag="ACG9CY_000698"
     CDS             21113..22393
                     /locus_tag="ACG9CY_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS4937232.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22603..23409
                     /locus_tag="ACG9CY_000699"
     CDS             22603..23409
                     /locus_tag="ACG9CY_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4937233.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23421..24641
                     /locus_tag="ACG9CY_000700"
     CDS             23421..24641
                     /locus_tag="ACG9CY_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937234.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24631..26217
                     /locus_tag="ACG9CY_000701"
     CDS             24631..26217
                     /locus_tag="ACG9CY_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4937235.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26256..28394
                     /locus_tag="ACG9CY_000702"
     CDS             26256..28394
                     /locus_tag="ACG9CY_000702"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4937236.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28762..29745)
                     /locus_tag="ACG9CY_000703"
     CDS             complement(28762..29745)
                     /locus_tag="ACG9CY_000703"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4937237.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30123..31514
                     /locus_tag="ACG9CY_000704"
     CDS             30123..31514
                     /locus_tag="ACG9CY_000704"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4937238.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31530..32570
                     /locus_tag="ACG9CY_000705"
     CDS             31530..32570
                     /locus_tag="ACG9CY_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4937239.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32589..33674
                     /locus_tag="ACG9CY_000706"
     CDS             32589..33674
                     /locus_tag="ACG9CY_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS4937240.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33707..>33943
                     /locus_tag="ACG9CY_000707"
     CDS             33707..>33943
                     /locus_tag="ACG9CY_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4937241.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVK"
BASE COUNT        10995 a         5821 c         6693 g        10434 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttattt
     4621 gaaattttat atctaatttt caattatccg cataaacact atacattcaa gcattttgaa
     4681 gtatagaaaa acactcatct taccacgaat ttaccacgat tgaatgagcc ttgatatgac
     4741 cgtaaaacta ctaagggaga tagcacgaaa ctgtgtatct cccgattttt ttatacagtt
     4801 gattacttca ctccgatttc aaatttcaat attgctttca tcatgtctgt atgaatttct
     4861 ccttttagtt cttcatcaac ctgcataatg attttaccac tttcagagtg gtaaaaagga
     4921 cgcaagcata atttagaaat ataaccgtca taaaacttca aaatctcttt aactgccatt
     4981 tcatctccgc ttgacgcttt gcaaatcaat tcaaaagagg gatagccata atctttactc
     5041 atttctttac ttcctttcgc tcaaaagcat ttttaacttc cttagtcctc tagttcttct
     5101 actgcaaata gcagaacgtg atacatggta cagttctgaa atttctctgt cgttcattcc
     5161 ttgaaaataa cgtaataaaa tcgcactacg ctgtttttct gacagtctat gtaaaactgt
     5221 accaagtgtt tcatcagata cataaattgt aaagtgcaag actttaaatg atacacaatc
     5281 ataaaaacag gtatcaatcg ttccacgttc aacttgtttc gtttcggcta tttcacaaaa
     5341 caatacttca cgtttagaat gtcttgcaag ctgtctgttt cgacttttca ctttgttctt
     5401 aatgacaatc agcattaaag aattgaactg taatctgacg atatgctcga aagaagatgt
     5461 cctcataatc tcaccccctt tctgctaatg ctgaaatata taaacggaat accgtttata
     5521 ccctttagaa atacagaaaa atggtaagaa aaaagcccac acaaaacata tatttgtgcg
     5581 agccttaaat actgcaatat tactctttct tttataacac tatcctttta gcagatttca
     5641 aattttcttt tatatattgt ttttagccta acatactatt taaccccttg tattatatct
     5701 ctaacttttg aaaaattctt tttgaatata ttgaaatcat gattgaagca atagaagcta
     5761 caaacataac aacccatgtt tcttgtataa atacatcgaa taaaataccg taaaccattt
     5821 gtccaatagg ttgaccgcac atagcaactg ctaccagcgt agccattatc tttcctacta
     5881 attgcggtgg tgtttgtgtc tgaacaagag tgtaaatttg aacaacaaac atagtagata
     5941 cacccatagc cacaaagcac atcactgtaa ctacccagta ggaaactatt gcaggcaatt
     6001 ctaaaaatag cgaaattccc ataagtaaaa cagaaacaga acaaataagt aacaaggtat
     6061 aaaggttttt tagttttaac tttgaagcaa caagagctgt taaaatacca ccacataaac
     6121 cacctaatga taatgctgat tgtgaaaaac catatagtgt atctggcatt tttaaaactt
     6181 cagaaattaa gataggcaga ccaacaatta aaacagcact taaaaccaaa ttgaaaatgc
     6241 aaaccaaaaa gattacggat ataaaaatgg gtttctcctt tttcaaaaag gtataactat
     6301 cttttaaatc gtttttagca actgcaatta cacctacttc acgaggtctt ttttggtgtg
     6361 gaatatggat aaaaatttcc ataatggctg aaaaagtaaa acatataata ctaacaatta
     6421 aaatgggatt gataccataa aattgaaaaa ctactccgct tatagctggt ccgataaagt
     6481 ttgctaaagt atttacttga tttattatag cattaccgac ggtaagttta tctgatgata
     6541 ccaatagagg aatacttgct tgtaccgctg gttgatatgc accagaaata ccataaagta
     6601 acatcaaagc aacaataaac aaaggtatga taggaatttt tcccaatgac aaataaaata
     6661 ctaaaattat gattgctgtt acaaaatcta gtatgaccat aatgtttctt ttatttgtcc
     6721 tgtcagcaat aataccacct accagcgaca aaataaccat aggtaaaaaa gagcaggctg
     6781 tagcaatacc aaacaaagct gatgaaccag tttcttttaa caaatagtac ggtaatgcaa
     6841 atcgtaggat agcgtttcca aataaagaaa ttatctgtcc tataacaaca agtataaaat
     6901 cacgattaaa aagttttttt tctgcttgca taaaatctct cctttttatt taatcaaaca
     6961 gaccgtcggt ttatataatg gtttaaataa actgctcttt tatagagcag tctatcgtat
     7021 agggataaca aatacagtta tttacaaaac tgtataaatg cagaaattgt ttcttcgtct
     7081 aataatgtgt ctatgcctat tccatttaaa agggcattga atgtattgca acgtttttcc
     7141 ataccctctt tatccgtcat ttcaactaat gggttgagcc atacattact taataccatg
     7201 ataacttctg caatttcatg tgaattttca atctccaaag acttatcctt gataccttgt
     7261 tctaaaacgg gttcaacaaa ttcaggtact acaatttcat acatttgttc tatttgcata
     7321 gcaagaaaac gaggattgtt taatagttga ggagaaactg tcaataaaac gctttggtta
     7381 gaattgaata ccgctgtctt aaatatttct cttaactttt ctaaaccgtt taaactatcg
     7441 ttatctctaa ttttagcaaa tattactgca ttttcatttc caattctttg aaaaattgct
     7501 tttaaaatat cttcctttga atcgaaatga tgataaatag cacctttaga aagttttgtt
     7561 tcattgataa tatcttgcaa tgatgtgtta tcatatcctt tttcacaaaa catttttgta
     7621 gaaacatcta aaatgagttt tactgtttct tctggatatt tatttcgtgc cataacttac
     7681 ctccaataca aaccgttggt ctgattaaag tatatcttag tattttatgc ttgtcaatat
     7741 aaagacttgc aattttataa aaatgcagaa tgcaagcctt tttcatttat tctacaatct
     7801 tccagttgct ttccttatgc ggtgtcaatt catactgtga tacctgcgtt gcctttgcct
     7861 gatttaccac aaatcagatt ttatcccttt cttcctcttg attgcccaca acagaaaaga
     7921 aaaagccctg tcaagagctt tgccaccatt ggtggtgctt tgcactcttg atcgggcttt
     7981 ttgtttgctg tatcttctcg caagaggaaa aaagttactc gtcctcatca tcaaaattgt
     8041 tattccttaa tacttctcgt aacagttcca tatcttcttt tgagttcggg tttatcattt
     8101 tcacgacttg ataaggtgtc ttatacttat gtccctctat gctttcccca aacatatcca
     8161 tactgtataa atcatcatag cggtctaata atttttgaaa cttcaaatgc tggataaatt
     8221 ctttgattgg gtaaaggtcg gacgcttcaa tgcttttccc gttgatatag tctgtaatat
     8281 attcccgtaa cttttctaac tcatattcca gccattcttc ctcgctgtca aagaagttat
     8341 catagtgtcc atgtttcagt tctgcattta gacgttcttc ggctcttaaa aactgacaga
     8401 cttccttttc agattgattg acatacttcc attctcccga caacaattca ttgggcgtaa
     8461 cgtccagcac ttccattatc ttttccagcg tttcataggc aggatagttc aaccctcttt
     8521 caatcttgga aaggctctgc atattgatac cgattttgtc cgcaagttcc tgttgtttca
     8581 ttcccctaaa ttttcttatg gtctgtatat tatttcctaa atggcttatt ttcattgcat
     8641 aaccctccat atctcgttat atcttcgcta ttataagtct atcatgctta atatgatttg
     8701 taaaggcaaa aaaagatatt gacaaataag catataagaa ttactatgtt cttgtaatcg
     8761 aataagcaca atagaatgaa atataaaaac attcaaaaag aaatacctat ctgattaaat
     8821 ccgtagatat gaatatcggt tgaaatggaa cttgttagaa aaggcaaaac agaacttgct
     8881 gacaaagtat ttctgacacg gtggcggaag cctctcaggc ttaccgccat gacaccttgt
     8941 gtcaagggga agaaatgctt tgtcggctgg cgagcctgcg agccttgtct gtgcccccgt
     9001 tatctaacag gggggcacta tttcacaaaa gacaagtcaa aaaccataca cccccaaacc
     9061 tgtgagtagt aatgagaggt tttaagaaga tagtacatat cacacttttt gaaaggtggt
     9121 ttacaggtat ttcaaggcta ttgtgggatt ggaggaatac aatgaacaat ttagaatttg
     9181 cacttgaaat gaagaacaaa cgtcttgcca gcggtctttc acaaggagaa cttgcaagtc
     9241 ttacccatgt atcaagatat agtatcaacc gctttgagaa cggaaaagct aacgccagca
     9301 aggaaacaca gaacattatc ctgcgttgtc tgaattacta tatctgcgat aagccttttt
     9361 atctgctcgt tgattatctg tctgtccgtt ttccaacgac tgacgcattg gaagttatcc
     9421 gaaaggtgct tggcatgaaa gcagattatt ttatccatta tgactatggg tattatggct
     9481 ataaggagca ttatgcttac ggggaaatca aggtcatggc ttctgatgat gaacacatgg
     9541 gcgtattttt agaattaaaa ggggcaggct cacgcaacat ggaatatgtc ttacaggcac
     9601 agaacaggga ctggtattca ttcttaaacc gctgtcttga ctgtggcggt gttatccgac
     9661 gttttgactt ggcaatcaat gatatgtgcg gtctgctgga tattcctgtg ctatctgaaa
     9721 aatacagaaa tggtggtgcg gaatgtcgct gtaaaaacta tgaaaatgta caaggtggaa
     9781 aattaagcgg aaaaaaccgt aatttagcaa gtacccttta tatcggctca aaggcaagca
     9841 caaaatattt ctgcctgtat gaaaaacaaa aggaacaggc aacgaaaaag aaacataccg
     9901 atattatcaa ccgttttgaa attcgtctgc gtgacaaaaa ggcggtacag gcagttgaag
     9961 aactgttatt gacgtataac cctcatgggc tggtgtttta tctcattacc gattttgtgg
    10021 aatttcctga ctatccattg tgggagatat tcatttccca tgacagttta ccttttgaaa
    10081 tgaaccctgt acctgtcaat atggaacgca ctctgcaatg gctggaaaga caggtcatgc
    10141 cgtcaatcgt gatgatagag gaaatcgaca ggctgacagg ttcaaactac atgaaaatga
    10201 ttgatgaatg tacccacctt tctgaaaaac aggaaatgct tgtggaacag atgtgtacgg
    10261 acatatcaga cgtaatcgaa agtgaggggg tgttttatga gtaatacaca ggatattccc
    10321 gtatgggaaa aatacaccct taccattgaa gaagcgtcaa agtatttccg cattggagaa
    10381 aacaagttaa gacgattggc agaggaaaac aaggacgctg gctggctcat tatgaatggc
    10441 aaccgcatac agattaaacg ccgacagttt gaaaaggtta ttgataaatt ggacgcaatc
    10501 taatgcaaat gagccttgta tgtgttatga tgaacacaag tcatatcaag gctctttcca
    10561 acaaggaaag gagcagacac catgaaagaa aaaagacggg ataacaaagg tcgtatcctg
    10621 catactggag agagccaacg aacagacgga aaatacttat ataaatatgt agacgcattg
    10681 ggaaacacaa aatatgtgta tgcttggaga ttgacaccca cagacccgac accaaaggga
    10741 aaacgggaaa aaccctcact tcgagaactg gaacagcaga taagacggga tattgaggac
    10801 ggtatcgaca gcacaggcaa gaaaatgacg ctttgccaac tctatgccaa acagaacgca
    10861 cagaggacaa atgtgaagaa aagcacacag aaacaacggg aacaactcat gcggttatta
    10921 aaagaggaca agttaggtgc taggagcatt gatacgataa aaccctctga cgctaaagaa
    10981 tgggcgttac gcatgaaaga caaaggcttt tcctacaata ccattaacaa ccataaacgc
    11041 tcgttaaaag cgtcattcta catagccata caagatgatt atgtgaggaa aaaccctttt
    11101 gatttcaagc taagtgaagt catagaaaat gataccaaag agaaagtcgc attgacagag
    11161 gaacaggagc aagccttatt gtcatttatt aagacggaca acgtgtatca caagcattat
    11221 gatgatgtgc tgatactgtt aaagacagga cttcgtatct cggaactgtg cggactgaca
    11281 gtagccgaca ttgatttcaa gaatgaagtt gtgattatcg accaccagtt actaaagagt
    11341 aaggaacagg gctattatat tgaaacgcct aagacgaaaa gcgggataag gcaagtgcca
    11401 ttaagcaaag aaacgataca ggcatttcaa cgggttatca acagccgacc aaagataaag
    11461 tcgatagagg tagacggaca cagcggtttt gtgtttatca atccgaaagg taatccaaaa
    11521 gttgccattg attataacgc cttatttatc cgtatggtaa agaaatacaa caagcaccac
    11581 aaggacaatc ccttgccaca tattacaccg catacgctac gccatacatt ctgcacaagg
    11641 ctggcaagca agaacatgaa cccgaaagat ttacagtata tcatggggca ttcaaacatc
    11701 agtatcacaa tgaactggta cgctcatgcg tccatagata ccgcaaaatc agaggttcag
    11761 cgtctaatcg catagaagta tttaccacga ttttaaccac gattgacagg gaaaatataa
    11821 gaagatagac ctagatatgc gaggtttacc acaaaagcaa aatgcccgta gagccgataa
    11881 aataaggctt tgcggacatt tgagaagata taaaaagata gtcaaaaaga catatataat
    11941 ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc ttttagtatg
    12001 gttataagat tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc
    12061 atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca
    12121 atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta
    12181 ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc
    12241 atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg
    12301 atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg
    12361 cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg
    12421 atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg
    12481 atttcattga tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct
    12541 acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct
    12601 tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt
    12661 atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct
    12721 ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa
    12781 gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt
    12841 ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct
    12901 ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt gattattgcc
    12961 atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag
    13021 tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag
    13081 aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct
    13141 tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc
    13201 aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga
    13261 gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct attttcatga
    13321 tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta
    13381 tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt
    13441 gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg
    13501 aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt
    13561 gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg
    13621 attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga
    13681 tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga
    13741 caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta
    13801 tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt
    13861 tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca
    13921 gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga
    13981 ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct
    14041 caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa
    14101 aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt
    14161 tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt
    14221 aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga
    14281 caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt
    14341 gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa
    14401 tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg
    14461 ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa
    14521 catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta
    14581 caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga
    14641 caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg
    14701 atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat
    14761 agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct
    14821 ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat
    14881 attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg
    14941 attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc
    15001 agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca
    15061 gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga
    15121 gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat
    15181 aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt
    15241 gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa
    15301 gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga
    15361 gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc
    15421 gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc
    15481 atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga
    15541 gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt
    15601 aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc
    15661 aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga
    15721 tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata
    15781 tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat
    15841 tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc
    15901 taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt
    15961 caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat
    16021 tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga
    16081 caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat
    16141 tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg
    16201 cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat
    16261 cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg
    16321 tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca
    16381 atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa
    16441 cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg
    16501 gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact
    16561 ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc
    16621 aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg
    16681 tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat
    16741 ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt
    16801 tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg
    16861 ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag
    16921 cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc
    16981 catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg
    17041 ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc
    17101 cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg
    17161 ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag
    17221 tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg
    17281 ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac
    17341 ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt
    17401 acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat
    17461 tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag
    17521 atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt
    17581 caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgaata
    17641 tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc
    17701 atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa
    17761 cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag
    17821 aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa cgaccgtgca ggacacgact
    17881 tgcgttatgc aatcgattcg acaaaattac gggaagagtt aggatggaag cctgagttta
    17941 cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt
    18001 ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag
    18061 aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt
    18121 taaaataaaa atcaaggagc ggataatcat atgtttttat taactggagg aaatgggcaa
    18181 ctaggtacag aattgcgcca tttattagat gaaaaaaatc tgcaatatgt ttcaacagat
    18241 gcaaaagact tggatatcac agatgccgag aaaacaatgg cttacatcac agaattaaaa
    18301 cctgatgtta ttttccattg tgcagcttac acagcagtag ataaagcaga agaagaagca
    18361 aaagaattag atgagaagat caatgttgat ggtacaagaa atgtagcact tgcagcaaaa
    18421 gctgtgaatg ctcaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa
    18481 aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa
    18541 ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct
    18601 tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc
    18661 cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg
    18721 gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg
    18781 aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa tattgaaaga tacagatgta
    18841 gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca
    18901 gtcatgagcc tgaaaaagac agaagagtta ggatttgtca taccaacttg gcaagaagca
    18961 cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt tatcaaatag
    19021 aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa
    19081 gcgaaccagg cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt
    19141 ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt
    19201 tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag
    19261 aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct
    19321 gaagaggact ttccgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt
    19381 gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc
    19441 tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg
    19501 gtcttgcttg gattcgaagt actgatgata ttaacgaaga gaaaaaaagc tctatcgcta
    19561 atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg
    19621 ggcgatttga ttttctttac tttgggactg atgactgctt tttatcatta tttctacaat
    19681 catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg
    19741 tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat
    19801 tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg
    19861 ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca
    19921 aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga
    19981 ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa
    20041 gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta
    20101 ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga
    20161 cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa
    20221 tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt
    20281 agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt
    20341 attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg
    20401 attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg
    20461 ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct
    20521 tttgccgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga
    20581 gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt
    20641 aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc
    20701 ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa
    20761 gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat
    20821 acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca
    20881 gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta
    20941 tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa
    21001 gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc
    21061 gtacagtaat aggctagctg agaataatcc tgaaatgtta gtagaaagga gagtggagat
    21121 aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt
    21181 actgacgcta gtcttttttg tttggacagc agggttaggt tttccagcaa ctttgtttat
    21241 atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt
    21301 ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt
    21361 cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt
    21421 gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat
    21481 ttatctgatt gtcttttcgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat
    21541 ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat
    21601 caatacgaat ataatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc
    21661 cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg ttgcagcttt
    21721 tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat
    21781 attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcttacac
    21841 ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa
    21901 tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag gagaaaaatc
    21961 tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc aatttttagg
    22021 aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc
    22081 tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg
    22141 gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac
    22201 attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg
    22261 atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat
    22321 gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca
    22381 acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt
    22441 tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta
    22501 tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg
    22561 cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat
    22621 ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac
    22681 ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta
    22741 tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat
    22801 gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca
    22861 gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc
    22921 gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccactta
    22981 ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg
    23041 tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg
    23101 ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg
    23161 cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt
    23221 gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt
    23281 ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg
    23341 ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat
    23401 gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat
    23461 cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga
    23521 aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg
    23581 agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta
    23641 cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc
    23701 tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg
    23761 gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat
    23821 ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg
    23881 ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag
    23941 cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca
    24001 agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt
    24061 gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag
    24121 taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata
    24181 aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt
    24241 tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag
    24301 taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa
    24361 cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc aagattcctt
    24421 ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg
    24481 aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga
    24541 caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag
    24601 accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat
    24661 gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag
    24721 tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg
    24781 agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga
    24841 ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat
    24901 tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa
    24961 gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat
    25021 aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta
    25081 gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg
    25141 ggattattca ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa
    25201 tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt
    25261 gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca
    25321 gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat
    25381 gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc
    25441 cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc
    25501 gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc
    25561 aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg
    25621 gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca
    25681 ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt
    25741 tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga
    25801 ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa
    25861 tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa
    25921 aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa
    25981 atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct
    26041 gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa
    26101 cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt
    26161 tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata
    26221 atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat
    26281 ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa
    26341 ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg
    26401 aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc
    26461 aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc
    26521 aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg
    26581 acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg
    26641 caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat
    26701 cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga
    26761 aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg
    26821 ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt
    26881 gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat
    26941 cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca
    27001 gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata
    27061 atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc
    27121 cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg
    27181 atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc
    27241 acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg
    27301 aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata
    27361 tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta
    27421 atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt
    27481 ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg
    27541 cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg
    27601 aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa
    27661 tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca
    27721 agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc
    27781 gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg
    27841 atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc
    27901 gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag
    27961 tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt
    28021 gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta
    28081 tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag
    28141 cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg
    28201 cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag
    28261 aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata
    28321 ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc gacttttcgt
    28381 tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg
    28441 ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag
    28501 gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca
    28561 aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac cgtttattcg
    28621 tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta ttcatgctca
    28681 gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg tcaaagaatc
    28741 gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt cctctacaaa
    28801 agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa ccagattgtt
    28861 cttagcgtca atataaagaa catttccact attaccaatt gctgaatata tttcgttctc
    28921 agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt ctaaagcact
    28981 ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt ttcctttata
    29041 catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg ataaacacaa
    29101 accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg ttttaggaga
    29161 cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat tgacatggtt
    29221 tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg ttagcttact
    29281 tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt tgaattcgcc
    29341 ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat cttcacttga
    29401 acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg ttagcaagtg
    29461 cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact ctggaaaata
    29521 atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa tgccaatcag
    29581 caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt ttttataacc
    29641 attccattca tctgtataaa cttctacccc atccttatat gcgacaattt gacatatatt
    29701 tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat attacctcac
    29761 tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat tcttcacgta
    29821 atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct gaaaaatata
    29881 aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct ttaatttacc
    29941 aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg tcttgattag
    30001 aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc ttataaacta
    30061 ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt aggagtgtat
    30121 atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata ttaatggatg
    30181 ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt ggagagatcc
    30241 cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg atttttattg
    30301 cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt gacatcgtgt
    30361 ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg acttttgcgg
    30421 gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc ggtacgcttt
    30481 tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt cgagataaaa
    30541 aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat tttggttcaa
    30601 agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat gcaaacatca
    30661 aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa gaaactgaaa
    30721 aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg aatacaacat
    30781 ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa agtattattg
    30841 aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg tttgatatca
    30901 tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca gcaattttag
    30961 tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca aaaaatcaaa
    31021 aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca aaatctggac
    31081 cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt attcgagctg
    31141 tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg tcgatcgtag
    31201 gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac ccttattact
    31261 acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat ggaaaatatg
    31321 tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa tattcattga
    31381 tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa atgtcctctc
    31441 aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt ccgaatgaaa
    31501 aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc ataatcgtac
    31561 cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa gaattggatt
    31621 atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca caggaaatca
    31681 ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc aatcaagggc
    31741 ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc ttattcttcg
    31801 atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg ctagaaaaga
    31861 gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac gagggaagaa
    31921 tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac gaagagttta
    31981 taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg aacaaatgct
    32041 atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat agttgtgagg
    32101 atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg aacccaaaag
    32161 tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac cgacctaaca
    32221 agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac gatattaata
    32281 aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt tctatttata
    32341 gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata ttgagtgatc
    32401 cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa aaaataacaa
    32461 agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt ggaagcttcg
    32521 ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag ttaatggagg
    32581 aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt ggagcagagt
    32641 ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt gattttttga
    32701 tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga ttaggaggcc
    32761 gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata gaagtgaata
    32821 agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt ggcgggggaa
    32881 ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat agccatgcaa
    32941 ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt ttaaccaaat
    33001 tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt gaaagtctgt
    33061 ttcaaaacca cccctttgag atagttcata atggtattga tttagagctt tatagtaaaa
    33121 attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt acttttgttg
    33181 tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta gagatttttt
    33241 tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa gggtcactta
    33301 gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta aaatttttag
    33361 gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta atgccttctc
    33421 tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta aaattagtct
    33481 tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt gtatcattag
    33541 atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct aataaaataa
    33601 cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag agtatatata
    33661 gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga aaatatgtta
    33721 tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc gattgaacga
    33781 aaatgcagtt tttattattc atatagataa aaaaactgat atagaaccat ttattgaagc
    33841 atttaaatca tatgataatg ttgaattttt atgtaataaa aaccgtgtta aaatttattg
    33901 gggtggtttt tccattgtac aagcagaaat caatttagta aag
//
LOCUS       DAZULG010000016        32945 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000016 DAZULG010000000
VERSION     DAZULG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.016"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(388..1854)
                     /locus_tag="ACG9CY_000708"
     CDS             complement(388..1854)
                     /locus_tag="ACG9CY_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4937242.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2145..4229)
                     /locus_tag="ACG9CY_000709"
     CDS             complement(2145..4229)
                     /locus_tag="ACG9CY_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4937243.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4371..4493)
                     /locus_tag="ACG9CY_000710"
     CDS             complement(4371..4493)
                     /locus_tag="ACG9CY_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937244.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            complement(4754..5113)
                     /locus_tag="ACG9CY_000711"
     CDS             complement(4754..5113)
                     /locus_tag="ACG9CY_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS4937245.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5143..5484)
                     /locus_tag="ACG9CY_000712"
     CDS             complement(5143..5484)
                     /locus_tag="ACG9CY_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937246.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5481..6155)
                     /locus_tag="ACG9CY_000713"
     CDS             complement(5481..6155)
                     /locus_tag="ACG9CY_000713"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4937247.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7331..8629)
                     /gene="asnS"
                     /locus_tag="ACG9CY_000714"
     CDS             complement(7331..8629)
                     /gene="asnS"
                     /locus_tag="ACG9CY_000714"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS4937248.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8669..9859)
                     /locus_tag="ACG9CY_000715"
     CDS             complement(8669..9859)
                     /locus_tag="ACG9CY_000715"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4937249.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9880..10407)
                     /locus_tag="ACG9CY_000716"
     CDS             complement(9880..10407)
                     /locus_tag="ACG9CY_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS4937250.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10454..13243)
                     /locus_tag="ACG9CY_000717"
     CDS             complement(10454..13243)
                     /locus_tag="ACG9CY_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS4937251.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13390..13590)
                     /locus_tag="ACG9CY_000718"
     CDS             complement(13390..13590)
                     /locus_tag="ACG9CY_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4937252.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14043..14363)
                     /locus_tag="ACG9CY_000719"
     CDS             complement(14043..14363)
                     /locus_tag="ACG9CY_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937253.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14866..15984)
                     /locus_tag="ACG9CY_000720"
     CDS             complement(14866..15984)
                     /locus_tag="ACG9CY_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937254.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16217..16339
                     /locus_tag="ACG9CY_000721"
     CDS             16217..16339
                     /locus_tag="ACG9CY_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937255.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            16455..17762
                     /locus_tag="ACG9CY_000722"
     CDS             16455..17762
                     /locus_tag="ACG9CY_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS4937256.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17883..19082
                     /locus_tag="ACG9CY_000723"
     CDS             17883..19082
                     /locus_tag="ACG9CY_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4937257.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19109..20377)
                     /locus_tag="ACG9CY_000724"
     CDS             complement(19109..20377)
                     /locus_tag="ACG9CY_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS4937258.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
     regulatory      complement(20496..20562)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(20604..21251)
                     /locus_tag="ACG9CY_000725"
     CDS             complement(20604..21251)
                     /locus_tag="ACG9CY_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS4937259.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(21530..22033)
                     /locus_tag="ACG9CY_000726"
     CDS             complement(21530..22033)
                     /locus_tag="ACG9CY_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4937260.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(22156..22920)
                     /locus_tag="ACG9CY_000727"
     CDS             complement(22156..22920)
                     /locus_tag="ACG9CY_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS4937261.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            23027..23302
                     /locus_tag="ACG9CY_000728"
     CDS             23027..23302
                     /locus_tag="ACG9CY_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS4937262.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            23979..24929
                     /gene="yidC"
                     /locus_tag="ACG9CY_000729"
     CDS             23979..24929
                     /gene="yidC"
                     /locus_tag="ACG9CY_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS4937263.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(25108..26082)
                     /gene="lacD"
                     /locus_tag="ACG9CY_000730"
     CDS             complement(25108..26082)
                     /gene="lacD"
                     /locus_tag="ACG9CY_000730"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4937264.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(26079..26537)
                     /locus_tag="ACG9CY_000731"
     CDS             complement(26079..26537)
                     /locus_tag="ACG9CY_000731"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4937265.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(26534..27997)
                     /locus_tag="ACG9CY_000732"
     CDS             complement(26534..27997)
                     /locus_tag="ACG9CY_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4937266.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(27979..28929)
                     /gene="pfkB"
                     /locus_tag="ACG9CY_000733"
     CDS             complement(27979..28929)
                     /gene="pfkB"
                     /locus_tag="ACG9CY_000733"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4937267.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            29072..29821
                     /locus_tag="ACG9CY_000734"
     CDS             29072..29821
                     /locus_tag="ACG9CY_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4937268.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
     gene            30088..31566
                     /locus_tag="ACG9CY_000735"
     CDS             30088..31566
                     /locus_tag="ACG9CY_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFS4937269.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(31634..32725)
                     /locus_tag="ACG9CY_000736"
     CDS             complement(31634..32725)
                     /locus_tag="ACG9CY_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4937270.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT         9810 a         6893 c         5679 g        10563 t
ORIGIN      
        1 gaataaatgg tgttcaatca gctgaccttc agcattagct caaaaagcgg caaaatatga
       61 ggagctattt ttccgtttgt ttctctaata ctcaggtcaa atcgctgatt ttacaacctc
      121 ttccttaaac ggtgttccaa agcttgcatc tgcggtaata agcccagcca aacgaaaaat
      181 atgaggggct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa
      241 cctctgattc acggtgttcc agaagtagct tcttcgaaaa acaagccgaa acttcacaaa
      301 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca
      361 caacctcttg taacaagtat ttctatttta aggctgaacc actgtttctg gttcagtgat
      421 ttctttgttt ttgtctcgac gagcaattgc tggcaagatc atacctaata agatcaagac
      481 gattggtgtg atgatattcg taaataattg gaaccaccat gctttaggat cagcagtata
      541 atctactttt ggcaccattc ctaggataca tgcaaacgca gtgaaggcaa agcaccaagc
      601 accaaagata aaaccgattt ttggattttt aacaaattta tattcagaat tgaatttctt
      661 ataagcttta ttcaacatca tatatgccaa gaacacccat aggtaacgca ttggcatgac
      721 aactgagttc aagttcgtca accattttac taattcattc atatctttga taccaaaaat
      781 cggcaacaaa ataatgattc ctactagtat acctgtcaga agataaccat tcaccaatgt
      841 acctttacga ttacgtttac gtagccagtt tggtacaaat tctggatccg catctgctaa
      901 caagattttt agtggtgcat caattgaaaa ggctaatgcg gagatttgtc caatcatgtt
      961 tgtcaatgcg taaatgatca tcaatgagtt accgatacca tagtaattac ctaaaatttg
     1021 aaaagcactg tatgccccat ttgccatcaa gtctttagga atattatcac tagcaaacag
     1081 catacccatt gcgactgagc ctaatacagc acaaatccct accattgctg ctaagaagat
     1141 catcccttta ggaaattcct ttgcaggatt tcttgtagag ttgacataag gggatatttt
     1201 ttccgcccct ccaactgcaa atactagcat cgaaacggtt gtgaaataac tgaaatcaaa
     1261 tgttggaata tatgttttca cttgattcat atgtggtgtg gcaaattcca tcgttggatt
     1321 gatgaacggt gccgttacag ctaaaagaat aaataagatc gacatgacga acatcgctgt
     1381 tccggccagt ccgccaatca ctttcaaagt agaaagtcct tttgttgata aatataaaaa
     1441 ggcaagaaat ataataagtg aaataactgc taccatttgt actgacatcg tatttacgat
     1501 atttccattt ccttggactg cccaaccacc agcaatcaga attgcttgag gcttttgtgc
     1561 caaataagga atatggacga cccagtaagt ccaagcggca tagtatgcca gtcgtttatt
     1621 agatgtcgta ttttcgaccc atgaactgac gcctccttta ctatccttga aggctgatcc
     1681 taattgtcca acgatcaatg cgtatggaat aaaataaatg gccaaaatca atagccaaga
     1741 ggtgacaact gatactcctt gctgagcata gttattaact acattcccca tgccccaaac
     1801 catgttaaat gcaattaacg caacagtaaa ccagcgtaat tgtttgttgt tcattcgttc
     1861 tctcctttat gataaaaatt tgttatccag atgtaatcat attcaatttt taacaaactt
     1921 acaagacagt gttattttat gtgtgtattc ttttatttaa tacacatcgt tttttcatat
     1981 tttttccatc aaaaaaataa gttattttct tttatttcat tgaccttatt gaatttcaag
     2041 ctattttgaa ttttctgcaa aattaaaaac attagttatt ttataatcct atctattttc
     2101 tttacatttt tcattaagaa aaggatataa aaaaaagagg acgttcattc gtcctctttt
     2161 tttacatgca agcttcgcat ggcattcaaa acagctaata ctgtcacccc aacatctgca
     2221 aatactgcat ctcccattga tgcaaaacca aaagcaccta aggcaagtac caaaattttt
     2281 atcccaatag caaagacaat gttttgtttg acgattttca atgttttctt tgctaaatgg
     2341 attgcttctg caatcttcga tggttgatca ttcatgatga ctacatctgc tgcttcaatt
     2401 gcagcatcgc ttcccaatcc acccattgcg attcctacat ctgctcttgc tagcaccggt
     2461 gcatcgttga ttccatctcc gacaaatgcg gtctttttat tgtttctttg taagatttcc
     2521 tctaaccgct gcactttatc ttcaggaagc aattcgctat atatcttgtc cacgcctact
     2581 tgcttcccga tatgatcagc aatttttttc gagtcgcccg tcaacatgac tgtgttcttc
     2641 acaccagcct gttttaattg aaccaatgcg tctacggcat cttctttcaa ggtatctgca
     2701 ataacaagat aaccactgta actttgatcg atcgctagat aaagcaatgt accgatttct
     2761 tgacttaaag gaaaagagat ttcaaatctt tccatcaatt tgtgattacc tgctaatact
     2821 tgtttgccat caacttcagc taagatccct tcacctgcaa attcctggat attagttgtt
     2881 ggtaaaagcg gacctgcgta gccttctaaa acagattgag caatcggatg tgtagaaaac
     2941 tgttctgcac tcgcaatata ttgcatgaat ttctttggat cgatttttgt atcgatcgtc
     3001 tggatcgaaa agtcaccttt cgttaatgtg ccggttttat cgaataccac tgtctcggtt
     3061 tgcgcaagca actccaagta atttgaacct tttactaata caccgatttt acttgcgcct
     3121 ccgataccgc caaagaaact aagtggcacg gaaatcacta atgcacatgg acatgagatc
     3181 actaaaaatg tcaatgctcg atagatccat tcagaaaacg ccgctccaga aacaacaagt
     3241 ggaggtaaaa cagctaataa aacggccaat cctacaacga ctggtgtgta atagcgcgcg
     3301 aatttagtaa tgaaattttc agctggtgct tttttactgc tggcattttc tactaattct
     3361 aataatttgc tgatagtaga attttcataa gatttttcta cttgaactaa aaatggttta
     3421 tttttattga taaatccgct taatactgtt tcaccaacat atacagattt agggactgat
     3481 tctcctgtaa gtgctgacgt atcgactaat gattctcctt cgatgatcgt tccatctaac
     3541 ggtacacgtt caccaggctt cacaagtaca aactgaccga tctgcacgtc ttcagggttc
     3601 acggtcatca atccttccgt cgtctgcacg tgagctgttt ctggacggat cgccatcaat
     3661 tcgcgaatcg attttcttga atgactgact gccaatcctt ggaaatactc gccgatttga
     3721 taaaatagca tcactgctac ggcttctgga tattcaccga tcaacatagc gccaatcgta
     3781 gcaacagaca taagaaaatt ctcatcaaag atttttcctt tgacgatatt catcactgcc
     3841 ttcttcacta catcatatcc tatcagcaga taaatcaata aataccctat caagcgaatt
     3901 ggttcattaa aatcaaataa tagcaataaa gcgatacctg caagggaaaa taaaatccgg
     3961 aacagcgtat ttctctcttt ttcatttcca tgttcagata ctttttcctt atctatttct
     4021 gttactttga catctggttc taattccttc actacttttt ttgtttcttg ttcaatcgta
     4081 gccaaatgat cttcctctgc gtcaatcgtt agttttccag aagtgaagtt taccgttgct
     4141 tccttgactc cattgatctt ctgaactcct ttttcgattt tcattgcaca gttcgcacaa
     4201 tctaaacctt ctaatctgta acttttcatt ttaatgacct cctcgcctca acatatgaac
     4261 atctcttcat aactaattat aaatgaatgg ttattcatat gtcaaggtat ccgaaagaaa
     4321 aaatgaaaaa catctgcatc ggaaaatttc ttccatacag atgttttggc ttattctttc
     4381 tttttctctt cgatcattgc ccctgctctt atccaacccg cacttgcctt cttgatcttg
     4441 acaggatagc aactaagttt gaagcctgtt gctggcaatt tgtctaaatt cattaatttt
     4501 tcgataggac aatatctttt ttcaatccct gcaaaatgtc cttcccagat attcgaagct
     4561 ccactacgct ttcaaaaata tagaaagagt tctttaatga aaattatttc aactttccat
     4621 aaacagtaat cccatataaa taaaagacgt aaaagtcaca tgggagaact tatacaggaa
     4681 aacacataga gtcaggtgaa atacatgtat cccacctgac tctatgcgtt ttctaacctt
     4741 tattattctc tttttataca atttctgcac cgaatggcat caacgccaat cgagcaagtt
     4801 tgaaagattg cttggcaaaa ggtatcccta tgatcgtgat acatagaagg atgccgctga
     4861 cgacatgtgc gagagcaatt ggtaggcctg aaacgatcag ccagatgata ttaacgatca
     4921 atgacatgcc agactggtga taaattactt ctttgccaaa aggcgcaaaa gaaagggagg
     4981 cgatcttaaa acattgcata ccgattggga tacctatgat cgtgatacac caaagtatcc
     5041 ccgctgctaa ccagctaaga ccaccgatta gtccgccgaa tataaaccag atgacatttc
     5101 ctaaacaact cattgtattt ctccttctat tactgttttt gattaaaact gggcaaaaat
     5161 tgattttatt cttctttttt tagcttccag catcaagtat tctgcaaatt ttttttggat
     5221 ttcttccgta ttttcttttg ttactaatgt gtgttggatt agttttcctg atggtcgctg
     5281 atccacccgt tcttctgact gttgcacgtc cgcttgcgca aatccgataa tccgtttttc
     5341 ttttgttaca gcaacgagca atagtttatt cttatcattt aggtcagctg ccgtttttgc
     5401 ttttacgaat gattctggat gatcagtaat cagctgattg atttgaggta aatcttcttt
     5461 tactgcccac cgtatttcca tcatttctca cttttctttc gctcattttt atctccacta
     5521 aaaaaatcga ctgcggcaat tgcatctatc cagctttgtg caatgatctc atgtccgcca
     5581 atcgttgggt ggaccccatc ttctcttgta tatttcgaaa aaccataagc aatccctaaa
     5641 gcattgaata tgccatctaa aggaaccagc aatgcgccat attccttagc aagtttacga
     5701 acaatttgga ttttagggtc taagtcttta cgccagccat gacggtcgtc ggttatagga
     5761 agtacaaacg gctccatcaa gatgatgttg cagatttccg ctgtttgaag ttcatttagt
     5821 aattttcgat aaacggattc aaattgctgc gcagatttct cagttccgaa tatagcctga
     5881 tcgctagtat tatgccaggt atcattgatt ccgatcaaaa tcgatacata gtcaggttgt
     5941 aaagctaaac aatcttcttg ccagcgtact tgcagatctg caattttgtc tcctcctatc
     6001 ccacggttca tgcattcaaa tttcatcgtt ggatattggg agattagttt tccggcaatc
     6061 aaaagtggat aacctttacc tagatcagtt gcttctgctc ggtctcttcc tgcgtctgta
     6121 atgctgtcac cgataaataa tatacgtttt ttcatttgtc aaaaactcct ttttgaataa
     6181 aacgatgatt ttcttcctta tattatccca taagccctat caagacgaaa gagaaaatat
     6241 atgtataaaa aatgacacca gcctagtccc ttcaatcaat tgaatgaacg ctcatcaagt
     6301 gtctagtttt ttatactttt tttcttaacg tgccggattc cccttgattc cttcgttgtt
     6361 tggtccagag aaatcgatca cacctaagct tccgtcaatc agaccactga tgacgccatc
     6421 agaacatata tttataggct catctaagac ttccagtaaa tgttctttta ctaatatctc
     6481 ttctagattg cttgggatag attggtcata actttatctg gtccgatgat ttcaaggatg
     6541 aattaaactt acatatggat cattgcatgc tgaaaatacg gacctactcc atcttcgaat
     6601 gttactatta gtttgttttc aggtactaga tgctgcttta tttcaacagc agaagcatca
     6661 tcaaatcaga tttccatttg aataaccttc tttgtatatg tttgattgat tgcgatttcg
     6721 ttatagaacc ttgtgaatca aatagcaatt tcttttgctt tcttctcttg tctgctggaa
     6781 acttccttta tcccgtattt taaaaaaaga actgcgagag ttcgcagttc ttttttttaa
     6841 ataaacgttg ctctccggcc aactcctcgg tcacaagtca caatagtcat caaacatgaa
     6901 aaactgtttt ttgaaattac tccttgtgac tcggagctga acggcaggcg cacaacctct
     6961 tgatataaac ggtgttcaat cagctgaccc tcgatattag ctaaaaaaac ggtaaaatat
     7021 gagaagctat ttttccgttt tgtttctcta atactcaggt caaggcactg atttcacaac
     7081 ctcttcctta aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa
     7141 atgtggaaag ccatttccac aaaatttttc ttaatactcg aaactaaaca gctttttcac
     7201 aacctctcca ctaaacggta ttcaaaagct tacatctgcg gtaataagtc ccgccaattc
     7261 aaaaacatga acagctgttt ttgcttggct gttctaatta cttgatgcag aacagctttt
     7321 tcatcacctc ttatggataa attcggttca acaaacgtgg gaatggaata gcttcacgta
     7381 catggtcgat accagcaatc caagtgactg ttcgttcaag gcctagacca aaaccagagt
     7441 gcggaacaga accgtattta cgtagatcaa gataccatga atattctttt tcatccaaac
     7501 cgtgattacg gatttgttct agcaagaaat catagtctgt tgcccgttcg cttccgccaa
     7561 taatttcacc gtaaccttct ggtgcgatca tatctgcaca aattaccacg tcttctcttg
     7621 ttggatgtgg tttcatgtag aatggcttga tggcttttgg atagtttagg ataaagacag
     7681 gctgttcgaa tgaattcgca atgaacgttt catgaggtga accaaagtct tcgccccatt
     7741 cgatgtcatc aaatccattt ttcttcagta attcgattgc atcatcgtaa gtaatacgag
     7801 ggaatggcaa ttttgtataa gtttttaaga cttctttgtc tcgtcctagt acatctaatg
     7861 catattcgca atgatctaaa acattttgta ctaagaatgc tacatattgt tcctgtactt
     7921 ctaaactttc ttcttgatga gtgaaagcca tttctggttc gatcatccag aattcgatca
     7981 aatgacgtcg tgtttttgat ttttcagcac ggaaagtcgg accgaatgaa aagacttttc
     8041 cgaaagccat agcagcagct tccatgtata attggccact ttgagataaa taagcttttt
     8101 gatcaaagta atttgtttcg aatagatccg tagtgccttc tggtgcagaa ccagtcaaga
     8161 tcggcggatc gattttgaca aaatgatgtt gattaaagaa ttcatacgtt gcacgaatga
     8221 tttcatttct gatctgcata atcgcatgtt gtcttgaaga acgcagccat aaatgacgat
     8281 gatccatcaa gaaatctgtt ccgtgttctt ttggtgtgat cggataatcg tggctctcgc
     8341 ttaccacttc cacatttgta attccgattt cataaccaaa ttttgaacga ctgtcttcac
     8401 ggatttctcc agttactaac acggatgttt cttggtgtag atttttagct gtttggaaaa
     8461 cttcttccga cacttcgctt tttacaacta caccttggaa aaaggctgtt ccgtctctta
     8521 attgtaaaaa tgcgattttt ccgcttgatc gcttatttgc gacccaagcg ccgattgtta
     8581 ctgtttctcc cacatggttt ttcgaatcga tgatttgaat ttgttccacg tatgactctc
     8641 cttttatatt ccttgaattt cttcaggatt aattttgact tttactctca ataaattgct
     8701 tgattctgcg taccgcttct tctaacgtag cgcgatcagt ggcataactc atgcggacat
     8761 tttctggtgc cccaaagcct tcgccagtca caagcgctac ccctgtttct tctaaaagat
     8821 cttctaccca ttgtgtgaca ttttcatact tacacatgcg tagcgtttct tttacattag
     8881 gaaaaagata gaaagctcct tgcggctttt caagagaaac acccggcagt tctgccacaa
     8941 gcggatataa cgtattcagg cgttcttcaa aggcgttccg catttcttct actgtatctt
     9001 gctcaccttt caatgcttca acagctgcat attgactgac tgctgtcgga ttgctagttg
     9061 attgggaagc aacagcgatc attccattga tgatcacagg atttcccacc gcataaccaa
     9121 tgcgccagcc tgtcatcgca tatgttttag acacgccgtt gatgatgatg gtctgatttt
     9181 tgattgcttc tgaaatcgtt gcaattggag taaactcatt gccattataa actaaacggc
     9241 cataaatatc atcagcaact atcaaaatat cattttttac tgcccattca ccgattgcct
     9301 gcagttcatt cttgctataa atcattccag tcggattcga tggagaattg atgattacgg
     9361 cttttgtttt ttctgaacgt gcttgttcta gctgttcaac agtaactttc cagctattgg
     9421 attcttcccc tttcacaaag acaggaagtc cttctgccag ttttacttgc tcaccgtaac
     9481 tgacccagta aggcacagga atgatgactt catcttgcgg gtcaaggatc gtttggaaaa
     9541 gggtatacaa agcaaatttc gctccgtctg ttacgactgt ttgtgaaggt tcatagacaa
     9601 gtccatagtt tttcttcaga taatcaacga ttgcctgtcg aagctctggg attcctgctg
     9661 ttggtgtata atagctagct ttcccattgc ggattgcttc gattgctgct tcttggatgt
     9721 tttctggtgt cgcaaaatcc ggttctccca cagtcaaact cagcacatct ctgccttttg
     9781 cttttaatgc cttggccttt gccgcagctg ccaaagtgac agacggttct aatcgttttg
     9841 ttctttttga taactccatc ccattcattc ctttaccttt taaattccct tgattgtctt
     9901 gacttcatct ccagattcaa agccaatcag gtaataattc agtttcccat ttgtttcttt
     9961 tcctactact tcccacacag gctgatcatc atacatccct aaagcagctt tttcgatttg
    10021 gatttcagga tgggccgcag caattgtctg ttttgcttcg ttttcagtca gtccctttgt
    10081 ttgatccaat acttttactt tttcaccaga tttaggaatg ataacggcga tttcttttcc
    10141 tttatcattt gtacctgtca gtgaaaagta tgtcttcttt cgagtaaacc aataaaaatg
    10201 atcggtttcc tcaagatttg catatttctt tgcgatttct actgcttctt tcttcgcctg
    10261 caccgccgga cgactggaac gcaagtaaaa aatcgagctg atcacgatga ccgctaataa
    10321 aaatacgatc aagccaagta acacgcgatt aatcgttttt tcctttgtct gttttttctt
    10381 caagggtcgt cgctccttta tcttcattcc ttttcattat agcagatttt aattatgaaa
    10441 aaagacagct cctctaactt ccttcttcat ttttctttaa gaattcatgg atatcctgat
    10501 agatttcctc catagctagc tctttgatcg gtactttttt cggtaatgca tggatgatcc
    10561 gtgcgccata ttttgcagtg actagtcttc gatccaaaat aagcaatacg ccacgatctg
    10621 tctccgtcct tatcaagcga cctaatgctt ggcgtaactt caaagctgct ttaggtacgg
    10681 attcctggta aaaaggattg atcccctctg attctaaata agcgttccta gctttgacca
    10741 tcgggcgaag cggattatcg aagggtaaac gtgtgactac aacgatctgc aaagcatctc
    10801 caggcaaatc tacaccttcc caaaagctgt cagcgccaaa caatacgctt ttttctgaca
    10861 aaatgaagcg tttcaaaagc ttctcccggc ttccaccaaa cccttgagct agtatttctc
    10921 gtcctttttc tagcatagac agatgcattt tctgatagac cttctgcaaa acatcatgag
    10981 aagtgaataa cacaagtatt ggccgatctt cttgttcgat cagacctgta agtgctgtct
    11041 ctaaataatc gatatatgct tgattgctga cttgatggac ggcaggtgcc tcttttggca
    11101 catagatcct tgcctgtttc tcataatcat aaggagaggc aatcacttta agaggtgctg
    11161 cttcaatgcc aagcctctta gagagatagt gtcgattagg gccgatcttc agcgttcctc
    11221 caatataaag aatacgatca tatcgaggat accaaacagt cgacggcaac atagccgctt
    11281 gaaaatcatg gagatgaaaa atcatctgct gcgctgtgtt cccaggaacg aaccaatgga
    11341 tcaaatgatc tcgccagtct tccagccatt gatccatcag caagtattgc ttgtctaatt
    11401 gatcaaatag gctatataaa cttgctaaat cgatccgcat ctggtttgtc cagctttctt
    11461 ggataatctc aaaagtcaga cgcatttgtg actgtagctg ctggatctcc tgataaaaaa
    11521 gctgcaggtg atcaatggct gtcccagatt ccagtggcag ctgatccaac atctcttttg
    11581 tcatcatgac ttcttctgga tactgtccgt taggttgctt gccgctcact aaactcatta
    11641 atccttctgc catacaaatc tgtgcttcgc atagcgcaga aagttcttct cgataaatcg
    11701 ataaccagcg taaggcttca tgttctttcg gtaaatagct ttgtattcgg tcaaacagct
    11761 gatttggttc ttctaattgc tggatttgtt tatggaaata gttaatactt aatcgtttgt
    11821 catttacttt ttcagcgatt tccggcaaat gatgtacttc atcgatcaac aaaaatggac
    11881 tagctggaag caatggttct tttcgctgtg tttcttgtac taaaaacgca tgattgacaa
    11941 tcaaaaaatt actttgcctg attttctttt gcaagaaacg aacaaagtct tgttcgtaaa
    12001 atgactgatt tcttgctaaa aatccaatac ctctgtgcgc gatatctgca aagaatgaat
    12061 ggttcaaatt tgttaaatgt aattcatcca aatcaccagt cttcgtttgc gtcagccaaa
    12121 ctaagaccgc catttgatac aaagcatatt gtttttgttc tggcggttcc acaagtgtgc
    12181 ctttaaaacg ctgcaaatca atatagtggc gatagctttt gataattaca ggttgtatgg
    12241 gctgatctag caattgattc agcaatggaa tgtctttttc tatgatctgc tgctgcaaaa
    12301 ctagcgaaac ggtactgatg acagcgggtt tttcaggtgt agccaaataa ctcaatggca
    12361 gcagataacc gattgtttta cccattcctg tagctgcttc gatcatcaaa tcttttgatt
    12421 catcttttgt gaaatggtcg tagaccgcat tcatcagtct gttctgttct tttcgataca
    12481 tcagtgtttt accaaacatt ttttcttttg cttgtttttt tcttggatat gtccgctcgc
    12541 cataatggac ggatgtgaat agttccactt cttttttctg caaagctagc ccatcgacga
    12601 tttctaatga gggatcaagt ggctctggtt tttctttcat ttcttcaatc acatgataaa
    12661 tgaaacgact cgtatccata cctgtgtgtc tacttaatcg cgcaatctgc tctaacgtga
    12721 tgattggcaa ttttctcatc cgttcttcga tcagcaaaag aagttgacct gtcacttcgg
    12781 catcactatc tgcctgatga ggattctcat gaataaatcc aaggctttct gacaaatcag
    12841 ctaagcgaaa gcttggttcc gtcggcaaga aaatttgtgc aagttctact gtatcgatgc
    12901 ctggtatctt caaactagga agtccgcatc gcatgagttc atggttcaaa aaattataat
    12961 caaaataaat attatgcgcg acaaatatgg tgtctgccaa aagattgtaa atcgtttggg
    13021 cgacatcttc gaaataaggc gctttttgga cgcgttgatt cgtgatccct gttaaatgct
    13081 ggatttgttt tgaaatcttc cgccctggat gaatatcgat cgaaaaacgt gaagtgatcc
    13141 gacctgattc aatcatgacg cagccaaatt ggatgatccg atcttctttt ggattcgtcc
    13201 cagtcgtttc gatatcaacg actgcataag ttcttggttt caaaatatct tcctcttctc
    13261 ctgcctctaa tgttcttttg aaattatact acaaagtgca gaaaaatact ctcatcgctt
    13321 tttaaagaaa atagacaaaa acgcccagac acgcaaatgt ctgggcgttt attttttagt
    13381 ataggttaat taagctttag taacgtttgt tgcttgaggt ccgcgttggc cttcttcaac
    13441 atcgaaagtt actgcttggc cttcttctaa agttttgaat ccgtcgcctt ggatagctga
    13501 gaaatgaacg aatacatcgt ttccgttttc tgcagtgatg aatccaaatc ctttttctga
    13561 gttaaaccat tttactgtac ctgtttccat aataaaaatc ctccttgtgt atcaacacat
    13621 attttgcaat tacttgaatg gtgtatatct ggaagatgtt tatttgaaac aagctaccac
    13681 agattaccga acaaaaacta ctcctatagt atatcatacc gtttacaaaa aagctagagt
    13741 ttttcgactt tttttattaa aaaaagtttt ctcagcgatt acataaagtg aaaaaccgaa
    13801 ctttttttta aaataaacga catattgttc agttggtgtt tttttgctct gatgttcttt
    13861 tttaaactaa aaaaattatt ctttctgact acttatcggt tctgtttcaa cggtcgaggc
    13921 tggggaagat tctttagtag gtctgatgtt ctcatggtat ttctgatagc ctatgacagt
    13981 taccagaaga acaaggcaaa ccaatacgac caacgagatt agtttttctt ttttgctcat
    14041 atctatacct cataaataac aggatgatgt ccatcatatt tctgagtatg agtaaaagca
    14101 aaagctgcag ctgtagaatg taagaaatgc cagcaacttt cataatcaca ggtcggacag
    14161 taccaaaagt aaccaaatct tggctgcaaa gaccgttctt ctgtttcagg aatcgcagaa
    14221 tcaagatctg gtgtctgtac ataaacattt ttgtacagac gtgtgcctgt aataggaaac
    14281 cgctgtgttt cttcagcaaa aactggctga cttgttaaac ttagggatag agacagcaaa
    14341 caccataaca gtcttttttt cataaagacc tccttatcga taagtttttc ccaattaaaa
    14401 tcatatctca agaacgcccg ttattcacat tttgttcata aatgatgttt tttgtatcat
    14461 aatcgtcaaa aagaggtcgt gaccacgtca caacctcttt tttaaacggt gttccagaag
    14521 tagcttcttc ggaaataagc cgaaatttca caaaaatttg agaaataatt tttgtcaatc
    14581 tcttcttatt tcttgaagct gaccacttct gtcacaacct cttccttaaa cggtgttcaa
    14641 aagcttgcat ctgcggtaat aagcccacca aacaaaaaat atgaagagct atttttgctt
    14701 gctgttctta ttacttgatg cagaacagct ttttcacaac ctctcataca cggtgtttaa
    14761 tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgtgaaacta tttttccgtt
    14821 tgtttctcta atactcaggt caaaacactg atttcacaac ctcttttaat aacgaagaat
    14881 cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc aaaataaaca cttttccgcc
    14941 attaatgatc acatcatctg ctaatgcatt caacacttga cgccgatcat attctgtttc
    15001 aggatcttca ccgaaaccat cagctaagtt tgacgtagcg ataaataaat gtgacacttt
    15061 tccttctttc gcagctggaa cgatatcaac taactgatcg atgaatttct tatctaagag
    15121 tttattgtat tcttttgatt cttgagctgc gagttcactt gacagcttct tcgccccttc
    15181 ttcgatatct ctaaaggata gctgcgcagg agaagcagaa atcgcaacgt ctgcacaata
    15241 atagggattt ttagcaattt ttttgaacat cgtctgattt tctggtagag caaataaata
    15301 aatcggccat tgttctggat tttcgaaaga atccttcaag tacgtatcaa ctgcttggta
    15361 atagttgacc caatcgatct ctacttcttc atcttttgga tttacgccat ggaaagttac
    15421 tccatctcgg gctccgcctc ctccattaga agagaagttc aaaccgccac ctgtcagttc
    15481 agatcccaat gcagtaacga catcaaccgg tgccccttct ggtagagata cttcttttac
    15541 ttgtttgttg ctgacttcat acaatttcat ggagtcgcgg ttcaatccca ataaataata
    15601 agaatagttg aactgcccat ttttgataat agccagtaaa taaggaagat cacttacata
    15661 gtattgatcg tctaccggga tactcaaacg atggatatag gtttggtcag cagttataat
    15721 gaccgctaca ctcgtagttg cactacgcca aaacgaagga tctcctaaca aattttcaat
    15781 tttttcttgg aacggggacc agtcatgttc ggaatatttt ttctcaaaac gtttctttgc
    15841 ttctttcgca aaatttttga atttgatctg atctttttcc acatcttgat gggcgacatg
    15901 cgtattcagc atgatcgtga cgaatggtcc tttagcgcct tctgaagtca attgtgacaa
    15961 taattcttta tccttttttg ccataaacgt acccctccta aaaaaatcat tcctttgttt
    16021 ctatctaaag tctaccacaa cactattttt ccatacaata aaacgtatct aaaaaaataa
    16081 aacggtttct acggttttaa atttagctat ttttttaagt tatcttcata atttcctcaa
    16141 ttatttagcc ccagtcttca actattcttc atgaaatgct gataaactaa gaaaaagaat
    16201 aaaagaggtg gatcttatgc atttgattat gattttcagt ttattaagta ttatcagttt
    16261 atttgctttc agcgcttccc gcttattaga tgcggaaaca cagcctattc ctgtaaaagt
    16321 acgtaaaaac gaccgctaac cttccataac gtgcgttctt atcctaaagt tttttcagaa
    16381 acattgaaac tctctcccct ctctgataaa attttaattt gtagcgaaac aaattaatta
    16441 caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt cctattattg gctagtctca
    16501 ttctttgcct tgcttctttt ctaccagctg ttgctgtgca agctgaagat actttcaaag
    16561 taaatgcaaa agcagccttt gctgtagatg cgcaaagcgg taagattctt tatgatcaag
    16621 atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat catcggtctt tacatcgtcc
    16681 ttgatcaagt aaaagaaggg aaactatcat gggatgataa agtttcaatt tcagattatg
    16741 cagaaaattt gagtgtcacc ccagatttat caaatgttcc tttgcataaa gaaaacacct
    16801 acaccgttaa agaattattc gactctgcaa tcatccagtc agccaacgct tcaatggttg
    16861 ccttggcaga aaaaatctct ggcagcgaag caaaattcac tgaacgaatg aaagaacaat
    16921 tgaaagattg gggtatcaaa gatgcaacga tcgttaatgc atcaggactg aataactctt
    16981 atcttggcga aaaccgacca gaaggtactg gagaaaacga cgaaaatcaa atgtctgctc
    17041 aagatgtggc tatcgttgcc cgtcatttga tcttggattt tccagaaata ctggatgtat
    17101 ctagcactac gacacaaatg tttggcgaaa acacgcaatc accagtagaa atggtcaact
    17161 ggaactggat gctcccagga ttcgtgaatt ataaagaagg cgtggacgga ttaaaaactg
    17221 gtacgactga acttgcagga gcctgtttcg tcggtacgat cactaaaaac gatcaacgaa
    17281 tcatcactgt cgtattgaat gcagacggcc atgcggaaaa cccaagtgcc cgtttcaatg
    17341 aaacagctcg cttaatggat tattgctacg acaattggtc agaaaaagag cttggaaaag
    17401 ccaatgcaag tatccctagt ctaaaaacca ttgatgtaaa agacggaaaa gaatcaacag
    17461 tgcccgttgt gcttaaaagt cctgtaaaag tatgggtacg gaacgatatg gatactggaa
    17521 aattaacaat cacaccaact cttgacaaaa aacaagtcac tgacaatgag atccaagcac
    17581 ctatggataa aggaacaaaa atcggtaccg caacgatcac cttggcagaa gataaattag
    17641 gttatcttga agacaacaac gcgccatcca cagaaatcat cacggataaa accgtagaaa
    17701 aagcaaatat tttcgttatt ggctggcgac gcgtcgcaga atttttcggt aacttgtttt
    17761 aattgaacaa aaagaaaaaa gcatctttcc atgtggaaga tgtttttttg ctttttctag
    17821 tttaaataac agcttttttc atctattttc gttatactgt ataagaaaaa ggagttgaat
    17881 ttatgaatct aaccagagat ctgatttttt tggttctcta tcttattggg gtattctcca
    17941 tcgtttattg gcgaaaacag aaaaaggatc cttccttgtt cttttacagc agtacatgga
    18001 cgtttgcggt aatcttgact tattttgtcg tgttggaaat tttttattcg accttttatt
    18061 ttttgattcc cttagtgttt ttcagtatct ttgccttttc ctatttttca gaaaagcgaa
    18121 ggctgttgaa tggtctttta ttcaatattt tcctcattag ttttggtatc tacttgtttg
    18181 tcttgctata cgaaacacaa aatatttttc ttggcggact gattgccttg atcacgatcc
    18241 cgttactgct tgtctttcta ttcggcattt atggattgat cgtttttctt ttttggaacg
    18301 gtgtcacagt tttacgtaga gagtcgcatt cattagctaa tcttttaacg ttgatactag
    18361 ccatttttct tacattgttt cttgtttttg actttttctt gttaaaatat ttaccacaat
    18421 ggataaatgc tttgtttttc tgcatcccat tgatcttgat ctatctattt atcgtattct
    18481 acaacttttt gactgtctca ttcttgtatc agttcaaccg ccctcgatac aaccaagact
    18541 tcatcgttgt gttaggcgcc gggttgatca atggagaaac agtttctcct cttcttgcca
    18601 agcgaatcaa taaggcgatt gctttttatt gggcacaaag ccgtgcgaca ttaaatccgc
    18661 ctatcctatt aatgtcgggt ggacagggag cagacgaaaa agtaccagaa gcaatggcga
    18721 tgaaacaata tgccatggaa caaggaattc ctgaaagaga tattcttgtc gaaaccaatt
    18781 cgacaaccac tttggagaat atgcgctatt caaaggaaat catggatcag caaatgaaag
    18841 gtccctatcg cgcgatcttc agttccaaca attaccacat tttcagagct ggtctctatg
    18901 cacgacaagc gaaactgaag gcaaatggca ttggggcaaa aacagctttc tattacttgc
    18961 ctaacgcgtt tttaagagaa tacatcgcca tcttgatgat gaacaaaaaa agacatatga
    19021 tcatttgcgg attgcttttc gcaggttccg cattcttatc actgatcact ttacttttct
    19081 aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt tttttctttc attctcttaa
    19141 ctattggcaa aaagagtatc ccttttctga ccgctacttg ctgagcagaa taaataccgc
    19201 tgttgcctga acacataaag ctgatcaagc agatcataaa gaagtatact gccgcttgtg
    19261 aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg tgtatttgtc gcaccagaaa
    19321 atactccgat aaagcccaat cctgcaagaa aaggaacaga aacatggagc aaaggcgcta
    19381 gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt cacttctccc ccttgatatc
    19441 ccgcgcctaa agacaaaact gtgaagaata acttcccaat aaaatcatag acatgtgcct
    19501 gtccattaaa tgattcctgc agtaatggaa gactcaatcc gagataacgt tgtgtttgca
    19561 aaatcaatgc tgcggctaca actacagccc cacctatcgt atttcgccaa acaggatgag
    19621 gaatccattg tgcatatcgc gcttttaaaa agacgatcga acggctgaat acccaaccaa
    19681 tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa cagcaacata ctccattcag
    19741 gaatcacacc catttggtaa tgtagatgag aaacaccata gctttctgtc ataaaatttg
    19801 caaacagtgc agcaaagaag ctagggaaga tcgcttctgt tcggactttc ccgattgcca
    19861 acacttctaa tccaaataat gttccagcta aaggtgtgcc aaatacagaa ctaaaacccg
    19921 cactgattcc actgataatc aagatttccc gttctgattt atccaatcgg aacaaacgtg
    19981 ctgtattatc tgctaacgct ccccccattt ggactgctgt tccttcacgg ccaacagatc
    20041 ctccaaataa atgggtagtt atggttccaa acagtgtaag tggaataagt cgcaacggga
    20101 ttttttcttc tccaccattt ccttgatcga tcactaagtt gtttccacga ctagaaagac
    20161 cgccgtgata cgcatagaga taagcaaaaa gcgcaccact aactggtagt agataaagga
    20221 gccaaggatg tgatagtcgg atatctgtca caactgtcag gctatttaag aaaaaagcag
    20281 ataagctccc catgaatata ccgagaatcg ctgtgatcac cagccacttc agcatgtaca
    20341 ctaaaatgcg tactggactc ggtaattgtt tttccataaa aaattcctcc caaaaaatct
    20401 gagccagaat gtggttcatt ctgactcaga tttgattttg cacaactcat cagtccttat
    20461 tgacaaagag ccctaaagaa aggggctatc cccagacttt agtaggcgtc attagctatc
    20521 tttcgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa agatagcata
    20581 tttttctgta aaaaacaata aaattatcgt tctttcttta cacgttcagc aaattcatag
    20641 aaaggagcca atttcgttaa ctgttgatta cattggtcac tatcatgaca aatataattc
    20701 ccctttttcg tatacgtgcc atcagaacct gctttcgttg tagaaagaaa aagcgacaca
    20761 ttcgatgttt tctgacagat tgcacagatt cccttattta tcgttggtga taaagtaccg
    20821 ctaatccctt ttaatttttc atcttcgtaa tagaccatga attttttctg cgtacctgga
    20881 tcattccagc cgatgtacgt atgttcttgc aagtcgattt cattccaagc tggttgtttg
    20941 agttttttca cttttcggaa taatttctcg atttgtggtt tgctaggctg cataaacggc
    21001 tggacgattt gcttcagctc ctccaatgct ctttctgctt gcgcttttgt cagttttacg
    21061 tctaaaactg tcgttaaaaa tgtatcgatt tctggcagtt cgtgttctaa tatgtctcga
    21121 atcttttcag ctgctagtgc ctgaactgtt tgtaccgttt ttggatcatt gacagatgag
    21181 tacgcattta ataactgatc tacttctctt tttataaaaa aatattggta tggttttatt
    21241 gtttgttcca tgattttaaa gtcctttctt ctaaccaaag tgcatacttt gtagaaaaaa
    21301 gtgatcttct gcaaagtatg tgttacctga agatcccgga ctcttttaaa taagatacgt
    21361 tttgcatctt atcatcaccc cctttaatgt tcattcttct atgtacatga ttcattcgat
    21421 aaataaaagt aaaccgtatc ttgaacagat agtcaactgc aaacagaaaa aaccgagaaa
    21481 atcagccaga ttttctcgga atatagtcta ttattattgg ttttacctat taaatcgagg
    21541 attctttccc aaagtaacgt tgccatctct tacgcatact tgcagacatg ggctgagccg
    21601 cttctttgac tgcgcagtat ttgtccacga atgtcacaat atatccttct ttatacttag
    21661 gcggtgcgat cgttgcaccc cacatgtgct tcaaaatgat atctcgctca agatccgaca
    21721 aatcggtgat tttttctgca ttctttacag caattcttgg atggatatac gcatgtgtcc
    21781 cctcgtcaaa tttcgtcgta cgccaatcat agtagaacag atcatgcaaa agtccagcac
    21841 gcgctgttgc tttagcattg ccaccccatt tttttgcgag cagataacta tagtaagaaa
    21901 cactgatgga atgctccaaa cgagtggaat gaacatgctg tgtgtaattc gctaattttt
    21961 tcactgcttc tgtttctaaa agatcttgta cataagacat atattcttcg tcttcacgcc
    22021 atttttctgt cataattatc gatctgccac ctttttgaat ttcattttct tctgaaattg
    22081 atctaagtat accaccagtg ttcggattat tctagtcgct ctagataaaa tagctaaagc
    22141 ttaatagttc cttaatcata aagaaaaatg ataaagaacg atccctgctg cgactccgac
    22201 attcaatgat tccgcatttc ctttcattgg gatatatacg ttctgatcag tcaaagacaa
    22261 gatttcctga cttactccct gaccttcgtt tcccatgatt atagcgaaat cctctgtttt
    22321 ttctactttg tctaatgcat gtgcttcttc atttaattct gtaccgtaca ccgtaaaccc
    22381 tttgcctttc attacaggga ttacatctgc caaatctttt tgtaacacag gcaaatgata
    22441 attgcttccc tgcatgcttc gcagcacctt tgtactataa atatctgccg ttcctttacc
    22501 taataaaaca ccagccatac caaacgcatc tgccgtacgg atcatcgttc ccacatttcc
    22561 aggatcctgt acattatcta gtaaaagcca gcctcctgaa aaatctggcg tatcttcaga
    22621 tgttaacgtc atcactgcta agatcccttg cggtgtaggt agatctgaca tagcggtcat
    22681 gacctcatct gaaactagaa aagtagaaat ctctttgttt acagccagcc agtctcccca
    22741 ttctttcata cctctctgtg tcacaaaaag ttcttttatt tctgcattag acgacgcagc
    22801 ttcttcgatc agatggaacc cttccaaaag atattctgcc ttttcttctc tgtatttctt
    22861 tttatgtagt ttttttaatt ctttgattct tggatttttt gctgattgga tctctttcac
    22921 attcctcacc tcaactgatt atatcatgga ataggtgctt gagcttgttt catcctaaaa
    22981 aaatcggtat cattgattta taaaacagga aagaaaggtg acgaacatgc gcaaagttaa
    23041 aatgaatgtc caaggacgtg tccagggcgt gggatttcgc tatatgacca agatggttgc
    23101 tgatcagtta ggcgtgaccg gaacggtaaa aaacgaagac gacggctcag tcacaatcga
    23161 agcaatcgga aatgacgaca tcatccaaaa atttatcgaa gaagtcaaga agtctccgag
    23221 cccaagtgga cgagtgcagt atgtggatgt ccaagaagat ccgatgattg aggaacgaaa
    23281 gaaatttgat gtagtgggat aaggttgtga aaaggtcgct tagttttgag tattaaggaa
    23341 aattttgtga aaatggcttt ccacattttt gcataaattt gacttaatac cgagaaactg
    23401 acttttgaac accgtcgata aggttgtttt tgaagcgctt tgcctcgagt attaaggaga
    23461 aacattttca aaatagcttc tcatattttg aaatgtaatg acttaatacc gagaggcagc
    23521 tgataaaaca ccgtcaataa ggttgtgaaa aagctggtct gcatcaagca ttaaggactg
    23581 gttgtgataa atagctctcc atatttttca acaggctgga cttaatgccg tagatgcaag
    23641 cttttgaaca ccgtcgataa ggttgttttt gaggcgcttt gcctcaagta ttaaagacag
    23701 gtagtaaaaa agctgatcta taacttgcaa tgaatttgct taacccatat tttccagaca
    23761 gaacaaaaaa gtttttagtt ttttaaataa gaaagaaaca cttggaatca ctcttttctt
    23821 tctaagtatt tctttcttga ttttttgtct tttttcaaaa tttttcgcta cgaaaaagaa
    23881 cgaatattta tatgaaattt ctatgaagtt gattgaattc catgcgcttt tttagtatag
    23941 ttaagtttgt gtaaaaatta tagaagagga agtattctat gaataaatgg aaaaactggt
    24001 tattgggatc cggacttgtc agtatcctgc tctttttatc tggctgtgtg cggatggatt
    24061 caaacggaaa ccctgacact tcaggaatcg tttatcgagt actcgtccat cctatgggcc
    24121 aagcgattac atatctagtt gagaatttca attggtctta cggctgggca gttattgcaa
    24181 tgactgtgat cgttcgtatc atcatcttgc cactaggtat cagtcaatcg aaaaaaacaa
    24241 tgatccaatc tgaaaaaatg caagcgttaa aaccacaagt tgaagcagca cagcaaaagc
    24301 taaaagctgc aacaacacgt gaagaacaaa tggcagcaca agcagaaatg cagcaagtat
    24361 atcgcgaaaa tggattgagt atgaccggcg gaatcggctg tctgcctcta ttgatccaaa
    24421 tgccgatctt ctctgccttg tattttacgg cacgctacac agaaggtatc cgggaatcat
    24481 ctttttacgg catcgatcta ggctcaccaa gtatggtgct tgtggccatc gcaggggtcg
    24541 cttatctgct tcaaggatat atttcaacaa tcggtattcc tgaagaacag aaaaagacga
    24601 tgcgtacgat gctgatcgtc tctcctgcta tgatcgtctt catgtcaatc agtgcgccag
    24661 ccggtgttac actttactgg gtagtcggtg gtgtgttcag ctgtctgcaa acattcatca
    24721 caaacgttat gatgaaacca cgtttgaaag cacaagtggc agaagagttg aaacaaaacc
    24781 cgccaaaaca agtcgtcact ccaagaaaag acgtgacaga atcagcaaag ccagctaaca
    24841 acaaacaagc aacaaaacaa atcaaacaac caaaaaacag tacaaacaac aaaggccgca
    24901 acgcaggaaa gcagcagaag cggaagtaga tagtaaataa gagtgtaata aaaattcatt
    24961 aacagtttta gcaattaaaa atatcgacca agatttttga aaaagaaatc tggtcgatat
    25021 ttttatttaa gtgaatgttt aatttgaatc atcgattgat cgctatctaa ttgcagcagc
    25081 atctttttat gaagaaacaa ctttttctta tctatcaacg tagacaggtg ttgctgtttc
    25141 ttctaatagt gtacttaatt ctttaatatt ttgttttcct tcttctaaca tccaactact
    25201 cgctttttct tcgccttcgg taacaaaagg tgccactccc ttcgcccatg ttgctcgccc
    25261 acataagaca ccattgaatg tcgaacctga ttgttttgca aaacgtaatg tatcttgaaa
    25321 tagcgtagca ttgactcccg cacttaaaaa aataaacggt tgttcagtca ctttcgattg
    25381 ttcatagaaa tacgcggatg cctctgcttg actataacaa gtctcttgcc catatccttc
    25441 cacaaatctc atattcactg gcacttctac tttcaataca tccacttgat aacgttcttt
    25501 tgaaaattct cgcatggctt caataacttt atgtggtttt acttttgcat aggcaagaga
    25561 agacggatcg ccaatagttg catcataaga aagaatctct aaaaagaaag gtatgccttc
    25621 tgcttcgcat tctgaaccaa tccgttctat aactatttgt ttttgcacat taatctctgc
    25681 tggttcatca acgtcatagt acaataaaaa tttacaagca tccgcaccag cttcttttaa
    25741 ccttttcacc gaccaaatat tcaataaatc aggtaatctt cccggtattt tcgtatcata
    25801 gccggttttt tcataagcta gcagtaagcc acattctttc tctcgaagcg ctgctgcagg
    25861 caggccgtat tctggatcaa gtaaaatagc tgaggcatag ggagtcaggt attgagaaac
    25921 catctgttta aattggatga tgtcttctga tgtagcttct ttatattggt tgatcatttt
    25981 cttcaaagca ccacgctggt cgattgctaa tgcgctaata attccagctt catccgtcaa
    26041 tcgcttcaaa cgttcctgtt tttctatact catcttcttc atagatcttc acctatttct
    26101 ttagttaata ttgtggctat ttctgttttt gattttgctt ttctcaactg ttctgtaacc
    26161 tcttttcgca tcaatgatcg tgcaacgaga gacaataatt tcaaatgagt cgttcctgct
    26221 tctgaatctg gaataaataa cgcaaaaata aaatcaactg actgtctatc taaactgttc
    26281 cattcaattc ctttgttcag tgtggcgata atcatactag gttcgttgat tgtttgagac
    26341 ttcgcatgag ggatagcaaa acctgacatt attcctgtag tcccttcttt ttctctttct
    26401 agaagttttt gatatacttc tttttcgtca agagcgatcc ctaaacagaa tgtttgcttt
    26461 gctaaaaact taaatgcttc ttcttgacta tccaatggct gttgtaaaaa aatgttctct
    26521 tcatgaatga ttgtcatgat aagtctcctg ctcttccttt ttcgataact gtacgatttg
    26581 ctgctttatt atccatcttc ttgtctttat cagttactct tttcttttgg attaatccta
    26641 gtatcactcc accaacgatc gaaccaataa gaatcgctgt aacccattgg atttttcccg
    26701 tcactacagg taacactaaa aaaccaccat gtggtgctgg aacttggacg ccaaacaaat
    26761 aagttaagac agcagcaatt gatgagccta acatcatcgt gggtaacact cggatcgggt
    26821 ctttcgcagc aaacggaata gctccttccg taatatgagt tgatcctaaa ataaaattaa
    26881 ccaaacctgc attacgatca ttggtatcaa aatatttacc aaacaataaa gtagcaaaac
    26941 cagtaatcaa cggtggtgca atacaagctg ctgacacacc agccataaaa gtcgtgtttc
    27001 cttgtgccaa aagagctgtt cctgttacat atgccgcctt attaaccggt cctcccatat
    27061 caaacgcaca catacagcca acaatcacgc caagaacaag tggattcgaa tgttcaaatc
    27121 cagctaaaaa gctcatcatt ccttcattta tagccttcat tggacctgcc agcaaagaca
    27181 tcacgatgcc aataaatgct acaccaataa ctggatataa gaagattgcc ttcaagccat
    27241 ctagttgttt aggtaaaatt gtcagtagct tttgcaaaaa taagataaaa taacctgcta
    27301 agaacccagc taaaatacct cctaaaaatc cagtaccgcc attcatggct acgattccac
    27361 caacaaatcc aacgactaac cctggacgtt tcgcgatccc ttctgcaata taagctgcta
    27421 aaactggaac catcattccc attgctgcat cgccaactgc ttttaccatt gccgctgtag
    27481 cattgtattg tgcatgatct ggatcaaaag aatagattcc ccaaacggca aatgaaatag
    27541 caatcaatac tccgccagct accacgaaag gcaacatatg agagacaccg ttcattaaat
    27601 ttttatagat gctacgacca aaattagcac cactcatcgc ttctggttct aaagtatcag
    27661 cagcttcatt atctgctaag acagtacctt ttcctgccaa tgcttcgtca atcagccgtt
    27721 gtgcatcctt gattcctgta ctgacagaaa catcaatgac tcgttttccc gcaaatcttt
    27781 ctgaatggac atctttatct gcggcaataa tgacagcatc tgcctgtttg atttcttcag
    27841 ccgttaattc attttcgatc ccaatttgtc catgggtctc cactttgatc ttgatgcctt
    27901 ttttcctagc tgcttgttct aatgcctctt gcgccatgta agtatgcgca attcctgtcg
    27961 gacaaccggt tgctgcaatt agttgatatt ttcccatgtt tcaaacctct cttccttgat
    28021 tttgatttgt ttacttaatt cctccacatc caaaaaatca gtcagtcctt tacgaaaagc
    28081 ggttgagctg cctgctgcta tgcttttacg taacgtttca tcaagcggtc gtcgattcat
    28141 atatcctgct aaaaaagcgc ctaacatggt atcacctgca catgctgtat tgacaacttt
    28201 tccttttggc gagtttcctc gaaacaccct gtctttcgta aaaagaatcg ctcctttctc
    28261 accgagggat aacaaaacat tttctgcacc taactctact agtcgttgtc cataaacaaa
    28321 gtaatcttca tctgtaacca tttttcgtcc gaaccaagaa gaaagttctt cctcgttcgg
    28381 tttcagcaaa aacggtcgat agggtaaaca atccatggct tcttgatcac tactatcaat
    28441 aatcaaatga atcccctttt cctgacaaat ccgactgatt tctattaaaa cctttggaga
    28501 cagccctctt ggtaaactcc cagaaacaca aagataatct tctgcttgta atgctcttat
    28561 ttgatttaaa aaattatgga cagctgtctg cggtatttta ggtccttgat tcactaattt
    28621 gtattcttct cccgtttgat tgacttgtgt aaatacattg atacgcgtca taccaggcac
    28681 ttcaataaac tctgtttgga tttgtttctg ctgtaaatag tcttctatat actttcctgt
    28741 aaacccagca gaaaaaccta atgccgtact tgggaaatct agcattttta atactagtga
    28801 cacattcacc ccttttccgt ttgcctgaat gtcatcctct tttgtgcggt ttaccataaa
    28861 aggatgcatc tcctctgttt caataaatag atcaattgcc aaattcatcg tgcatgtgta
    28921 gatagacata accaacgacc tccttgactc tattatgaaa tacgcgaaag agaaaatgtt
    28981 ttcttaatcg tcccaacaca aagaaaacct ttacatttat aaactcgtcg tgctattttt
    29041 taactaactt ttggtgaaag aaggaaagaa tatggatcac aaattaagtg aaacagaaca
    29101 gtacctctgg aactttatcg aacaccacat cctagagatc cctaactact ccatcgtgaa
    29161 actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga aaaagaaagg
    29221 ctatgaagga tttacttcgt ttaagcacca tctaaaagaa ggagaaaaca agaacatcaa
    29281 tttttctttc ttagataaag tagacaaagg tatccgtcga gccattctaa aaaatgaaca
    29341 agaagtggta cgtacgatca atatgttgga aatcggaaat attgaggatg caatacaaag
    29401 aatcaaattt gctgaccggg tttttgtctt tgcacgagga ttttcagaaa tgattggtca
    29461 agagatgctc gtaaaacttc aattaacagg gaaaaattgt caaatgcata ctgatccaga
    29521 aatcatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt ttgtttcttt
    29581 gaacggcgag acaaaagaac tcgtcaccgc cgcaaagaat tgctatgatg cagagattgg
    29641 ttcgattcta gttacagcca atcattcctc tacactgaaa gactattgcg agatcaatct
    29701 cgtaggtttc aaatcagaag gttcttattt ccctgattat gaagtacggt cacgtttgcc
    29761 tctccagatt atagcgagaa tcctgcttga cgcctatgta ttgcgtatgg gggagaattg
    29821 aaaagcagtt tattatggtt ggttacagtt taaaacatca cattttttat tctttatatt
    29881 gtatggactc acggaaactt tttatgtata aagttattca caatctataa agttacttta
    29941 aataacaaaa aaataattga aaatacttaa aatcaaaaaa tatcttttta atcaataaaa
    30001 aaattgacag ttttattaat aataagctat tctgttatag aagaaaaaat tgtttgttat
    30061 tattagcaac aaaaaaagga gtgcatcatg aaaaagttat ctcaaaaacg tttagctcaa
    30121 atcattattg attctagaaa atctctgaaa atgacacaac aacaacttgc agtagctact
    30181 ggtatcaacc gtgccttaat tagtcgatta gaatctctcg attttatccc gtcaatacaa
    30241 caattagaat cattagcaga agttctaaat tttgaaccaa cagatctatt tataaatgaa
    30301 tcaaataaat caaacagaac aaaacatcag tcgaagaata ttgctgtagc cggtactggt
    30361 tacgtaggtc tttccattgc tactcttctt gcccagcaca atcaggtaac agcagtagac
    30421 attcttcctg aaaaagtaaa acaaattaac aataagaaat ctccaataca agacgattac
    30481 attgaaaaat atctagctga aaaaaatctt aatttaattg caacgactga tggtgcagcc
    30541 gcctatagag aagccgattt tgttgtcatt gctgctccaa caaattatga tagtaaacaa
    30601 aattattttg atacttctgc tgtagagtca gtaatcaaat tggtgttaga agtcaatcca
    30661 aaggcaataa tggtcatcaa gtcgacggta cccgtaggct ataccgaaag tattcgtaaa
    30721 aaatacaata ctaaaaatat tctattcagc ccagaatttc tcagagaatc taaggcatta
    30781 tatgacaatc tatatccttc gcgaattatt gtcggcacgg accaaactga caaaacttta
    30841 cgagaagcct ctcacacttt tgcagattta ctccaagaag gagcaatcaa aaaagatatc
    30901 gaaactcttt tcatgggatt cacagaagct gaagcagtta aattattcgc taatacttac
    30961 cttgcacttc gagtatctta cttcaatgag ctggatactt atgcagaaat gaaaggacta
    31021 aatacacaag aaatcattca cggggtttgc ttagatccaa gaattggcac acattataat
    31081 aatccatctt ttggatatgg aggctattgt ttaccgaaag atacaaaaca acttctcact
    31141 aactataacg atgttcctca gaatatgata agtgcaattg tcgatagtaa tcgtacaaga
    31201 aaagacttta tagcagatcg agtgttagaa cttgctggca cgtatgaggc taacagttcg
    31261 tggaatgcag aaaaagaaaa agaggtagtc ataggtgtat accgtcttac aatgaagagt
    31321 aattcagaca acttccgtca gtcatctatt caaggagtta tgaagcgaat taaagcaaag
    31381 ggggcaacta ttgtaattta tgaaccaaca cttgaaaata acactacatt ttttggttca
    31441 aaagtaataa ataatttaga tgattttaaa aatatcagtc acagtattat tgctaatcgt
    31501 tacgatagta atcttgatga tgtaaaagaa aaagtatata ccagagattt atttcaacga
    31561 gattaaaaaa taacacctca gatttgtagt gtctgaggtg tttctataaa cttttataag
    31621 taaaaaaata caattatact tcataaaaag acttatacca ctgcgcaaat ttacgaagcc
    31681 cgtcacgcaa acttgtactt ggtttaaatt caaaatctcg ttctaacgca ctagtatctg
    31741 cataagtaac tggcacatct cctggttgca ttggaactag ttcggtatga gattcaaagt
    31801 cgtaatcttc tggtagcacc cctgctgcaa ttagctcttg ttgtagaata tctacaaaat
    31861 ctagtaggtt ttccggcgta ttgttaccaa tattataaat atgatatggt gggattggta
    31921 gcccatcttc cccatcaatt ttctctggag cgcactgcat aaccctcttt actccttcaa
    31981 caatatcatc tatataggta aagtcacgct tgcaattccc ataattgaat atttggatat
    32041 tatcaccagc acgaagttta tcagtaaaac caaaataagc catatctgga cgcccagcag
    32101 gaccatatac tgtaaagaaa cgtaaccctg tagacggaat attgtaaagt ttagaatatg
    32161 catgtgccat tgattcatta cttttcttag ttgctgcata caacgatact tgattgtcta
    32221 ctttatcctc tgtagaataa ggaattttct tattagtacc ataaacggaa gacgatgagg
    32281 catacactaa atgttccaca ccagattctc cattatcata tgaatgacga caagcttcaa
    32341 gaatattata gaatcctatt atgttagatt cgatatacat atctggatta gtaattgaat
    32401 aacggactcc tgcctgagct gctaaattca ccacgatact tggtttataa ttatcaaaga
    32461 tgaaatcaat tgttgcttta tcagcaatag aacctttgat aaaattccaa gtagatcctg
    32521 gatggttctt tgcctccgtt tctatctctg ataatctata gttcttaata gagacatcat
    32581 agtaatcatt catattgtca atgccaatga tttttattga tgactgagta cgtaataact
    32641 caagaaccaa atttgatcca ataaaaccgg ctgcgccagt tattaatatt gttctatttg
    32701 ataaggacgt gtttaattcc agcatttttt agcactcact ttcttttttt cttatgaata
    32761 catatttgtt atatattaaa aaatcttatt tagacttatc tattgtcact tttatcattc
    32821 taatctttgc acgtagtaga ataattatac agataggaaa ttgctaagca acccacttct
    32881 ttctattatc tattaactta ttctaaaacg aatagaaatt attcttgatc acataagtaa
    32941 cctta
//
LOCUS       DAZULG010000017        32540 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000017 DAZULG010000000
VERSION     DAZULG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.017"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..308)
                     /locus_tag="ACG9CY_000737"
     CDS             complement(<1..308)
                     /locus_tag="ACG9CY_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4937271.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     S"
     gene            complement(326..646)
                     /locus_tag="ACG9CY_000738"
     CDS             complement(326..646)
                     /locus_tag="ACG9CY_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4937272.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            940..1080
                     /locus_tag="ACG9CY_000739"
     CDS             940..1080
                     /locus_tag="ACG9CY_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937273.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            1378..1635
                     /locus_tag="ACG9CY_000740"
     CDS             1378..1635
                     /locus_tag="ACG9CY_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFS4937274.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(1606..1914)
                     /locus_tag="ACG9CY_000741"
     CDS             complement(1606..1914)
                     /locus_tag="ACG9CY_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937275.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1992..2738
                     /locus_tag="ACG9CY_000742"
     CDS             1992..2738
                     /locus_tag="ACG9CY_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4937276.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            2753..2956
                     /locus_tag="ACG9CY_000743"
     CDS             2753..2956
                     /locus_tag="ACG9CY_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4937277.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            3061..3360
                     /locus_tag="ACG9CY_000744"
     CDS             3061..3360
                     /locus_tag="ACG9CY_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937278.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            3360..3530
                     /locus_tag="ACG9CY_000745"
     CDS             3360..3530
                     /locus_tag="ACG9CY_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937279.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(3589..4068)
                     /locus_tag="ACG9CY_000746"
     CDS             complement(3589..4068)
                     /locus_tag="ACG9CY_000746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937280.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            4113..4820
                     /locus_tag="ACG9CY_000747"
     CDS             4113..4820
                     /locus_tag="ACG9CY_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS4937281.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            4813..4941
                     /locus_tag="ACG9CY_000748"
     CDS             4813..4941
                     /locus_tag="ACG9CY_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937282.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            4999..5340
                     /locus_tag="ACG9CY_000749"
     CDS             4999..5340
                     /locus_tag="ACG9CY_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937283.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            5333..6079
                     /locus_tag="ACG9CY_000750"
     CDS             5333..6079
                     /locus_tag="ACG9CY_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4937284.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            6085..6771
                     /locus_tag="ACG9CY_000751"
     CDS             6085..6771
                     /locus_tag="ACG9CY_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4937285.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            6778..7611
                     /locus_tag="ACG9CY_000752"
     CDS             6778..7611
                     /locus_tag="ACG9CY_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS4937286.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            7628..8479
                     /locus_tag="ACG9CY_000753"
     CDS             7628..8479
                     /locus_tag="ACG9CY_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4937287.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            8476..8835
                     /locus_tag="ACG9CY_000754"
     CDS             8476..8835
                     /locus_tag="ACG9CY_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS4937288.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            8850..9011
                     /locus_tag="ACG9CY_000755"
     CDS             8850..9011
                     /locus_tag="ACG9CY_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4937289.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            9008..9313
                     /locus_tag="ACG9CY_000756"
     CDS             9008..9313
                     /locus_tag="ACG9CY_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937290.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            9313..9630
                     /locus_tag="ACG9CY_000757"
     CDS             9313..9630
                     /locus_tag="ACG9CY_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS4937291.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            9627..10013
                     /locus_tag="ACG9CY_000758"
     CDS             9627..10013
                     /locus_tag="ACG9CY_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS4937292.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            10010..10345
                     /locus_tag="ACG9CY_000759"
     CDS             10010..10345
                     /locus_tag="ACG9CY_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937293.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            10345..10479
                     /locus_tag="ACG9CY_000760"
     CDS             10345..10479
                     /locus_tag="ACG9CY_000760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937294.1"
                     /translation="MKDWLEEASALVGCFINCAFISVIIALVGFVSLKVIILLWQLIF
                     "
     gene            10491..11060
                     /locus_tag="ACG9CY_000761"
     CDS             10491..11060
                     /locus_tag="ACG9CY_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS4937295.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            11057..11230
                     /locus_tag="ACG9CY_000762"
     CDS             11057..11230
                     /locus_tag="ACG9CY_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937296.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            11227..11532
                     /locus_tag="ACG9CY_000763"
     CDS             11227..11532
                     /locus_tag="ACG9CY_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937297.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            11556..11753
                     /locus_tag="ACG9CY_000764"
     CDS             11556..11753
                     /locus_tag="ACG9CY_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFS4937298.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            11750..11956
                     /locus_tag="ACG9CY_000765"
     CDS             11750..11956
                     /locus_tag="ACG9CY_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937299.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            11956..12252
                     /locus_tag="ACG9CY_000766"
     CDS             11956..12252
                     /locus_tag="ACG9CY_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937300.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            12328..12741
                     /locus_tag="ACG9CY_000767"
     CDS             12328..12741
                     /locus_tag="ACG9CY_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4937301.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            13425..13592
                     /locus_tag="ACG9CY_000768"
     CDS             13425..13592
                     /locus_tag="ACG9CY_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937302.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            13618..13962
                     /locus_tag="ACG9CY_000769"
     CDS             13618..13962
                     /locus_tag="ACG9CY_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4937303.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            13967..14248
                     /locus_tag="ACG9CY_000770"
     CDS             13967..14248
                     /locus_tag="ACG9CY_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937304.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVDIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            14351..14665
                     /locus_tag="ACG9CY_000771"
     CDS             14351..14665
                     /locus_tag="ACG9CY_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS4937305.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            14643..16337
                     /locus_tag="ACG9CY_000772"
     CDS             14643..16337
                     /locus_tag="ACG9CY_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4937306.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            16357..17535
                     /locus_tag="ACG9CY_000773"
     CDS             16357..17535
                     /locus_tag="ACG9CY_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4937307.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            17498..18184
                     /locus_tag="ACG9CY_000774"
     CDS             17498..18184
                     /locus_tag="ACG9CY_000774"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4937308.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            18184..19344
                     /locus_tag="ACG9CY_000775"
     CDS             18184..19344
                     /locus_tag="ACG9CY_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4937309.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            19354..20229
                     /locus_tag="ACG9CY_000776"
     CDS             19354..20229
                     /locus_tag="ACG9CY_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937310.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            20226..20537
                     /locus_tag="ACG9CY_000777"
     CDS             20226..20537
                     /locus_tag="ACG9CY_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937311.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            20527..20880
                     /locus_tag="ACG9CY_000778"
     CDS             20527..20880
                     /locus_tag="ACG9CY_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4937312.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            20870..21271
                     /locus_tag="ACG9CY_000779"
     CDS             20870..21271
                     /locus_tag="ACG9CY_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937313.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            21264..21668
                     /locus_tag="ACG9CY_000780"
     CDS             21264..21668
                     /locus_tag="ACG9CY_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937314.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            21680..22285
                     /locus_tag="ACG9CY_000781"
     CDS             21680..22285
                     /locus_tag="ACG9CY_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4937315.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            22305..22667
                     /locus_tag="ACG9CY_000782"
                     /pseudo
     CDS             22305..22667
                     /locus_tag="ACG9CY_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22670..22852
                     /locus_tag="ACG9CY_000783"
     CDS             22670..22852
                     /locus_tag="ACG9CY_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937316.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            22870..26289
                     /locus_tag="ACG9CY_000784"
     CDS             22870..26289
                     /locus_tag="ACG9CY_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4937317.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            26340..27077
                     /locus_tag="ACG9CY_000785"
     CDS             26340..27077
                     /locus_tag="ACG9CY_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4937318.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            27087..29378
                     /locus_tag="ACG9CY_000786"
     CDS             27087..29378
                     /locus_tag="ACG9CY_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4937319.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            29402..31483
                     /locus_tag="ACG9CY_000787"
     CDS             29402..31483
                     /locus_tag="ACG9CY_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937320.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSITSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SDYTFAITYTI"
     gene            31500..31649
                     /locus_tag="ACG9CY_000788"
     CDS             31500..31649
                     /locus_tag="ACG9CY_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937321.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            31646..32092
                     /locus_tag="ACG9CY_000789"
     CDS             31646..32092
                     /locus_tag="ACG9CY_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937322.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            32094..32231
                     /locus_tag="ACG9CY_000790"
     CDS             32094..32231
                     /locus_tag="ACG9CY_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4937323.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            32269..>32540
                     /locus_tag="ACG9CY_000791"
     CDS             32269..>32540
                     /locus_tag="ACG9CY_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937324.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT        11736 a         4921 c         6817 g         9066 t
ORIGIN      
        1 gattcatcag tcagttcgtt ctcaatatag atatttaata aaattagaat agctatttta
       61 tctgcttctg tttcaaattt tgagtgaaaa gttgtagaag tatcgtacaa aactgaaaat
      121 tcaaaatgag aggcgctgaa atgtgcaagt tcatgagata aatgaaaggc ttctgcagtt
      181 tcgccatata gattttcatt caaaaaaatg attctaggct tcggataata gaagccaggc
      241 tctttcattt ccatgtaaac tactttcaaa ttgtattcgc tcaacatttc tttcaatttc
      301 aaatacatac aacccatcac tccaactatt cattttcctc taaagcttta gcgattgcaa
      361 tcgctttacg cattgtctcc ttagatattt cttttccatc aaaagaaaaa acagtatcgt
      421 tttctgataa atccacatgt ttaggggttt ctttttcttc gcgccccaga agatagtcta
      481 cagagacatc gaaatagtca gcaatttctt gcaaacgttc tgtagagggg tttgagttct
      541 taagtctata taagacgttt ttaccatagc ctaggtcttc ttcaacttta tttaatgatt
      601 ttccctgttt tttcgctaac tcttttattc tctcaaacgt tatcatatca atattccttt
      661 cgttcaagaa aaaatattta actttatttg ttaaaattcg ttgactattt taacaaaagg
      721 tgttaatatc attcttgtaa acaagtttaa caactaaaaa gacaacaaaa aacactattg
      781 attaataaat gccaaccgcc aagaaagctt ttaaaatcaa tgtttatatg tcttatttaa
      841 ctatgttctg attttaacac tgtatgttaa tttagtcaac gcagtttttt aaaaagttgt
      901 taaatttgtt tacgaatata aaagaaagga gagaagaata tggaaaaaac agtctcggca
      961 aaaatcgaag atttgaaatt agacatactg aaacaagcaa aagtggcgat ggaacacgcg
     1021 gtagataaag aagactctgc catggttgca gccatagcag agattttagc tcacgtttag
     1081 tcatcgtttt tacggtcttc tggaagctga ccatagataa cagcataatg aatatctaaa
     1141 taagtttgaa caatttcttt tggcgaaatt gattcgcctt tagtagatac ttgtgattcg
     1201 tgatttagcc atgcaacaac taaatccgca gcgatttcaa acggcgttac actttgtgtc
     1261 agttgccata aagaatttca caatatgtat ggtcgtggta ataataccaa agagcaattt
     1321 gaggaattta tggaaattaa aaaatacaaa aaatcattta ttaaaggagg ctaaacaatg
     1381 aacaaggaaa tgagccaaac aatcaaggta caaaaaatga tagatgatct aacacatgga
     1441 attgatagcc aagccgataa aatcatcaag gaattgcaag gacaaaaagt taaggatgca
     1501 aaaatgcttc tcaagactat caactttgaa atgaatccaa caaaaagaaa gctcgccgat
     1561 gtgttggaag aaaagttggc ttccgctatc aacgagcaag aattactatt tgaaaccgat
     1621 acttttaaca gctgatgttt tatagatgac agtttcatcc gaatctatat catcgccgag
     1681 agaaaagaat gttgcttttg acaaaaagcc gataagacct acaagcggtc ttgatgttgc
     1741 aagagtagat accccaatat caccttgcaa ggtattcacg taaatttgag aagaatagaa
     1801 cattccatct ttttcttcga tatttgacgc atcatcgtat ccaacgacca aagtatcggc
     1861 aaatattttg attttttctc catcaagcat agtaattaca aattctggtt tcataaattt
     1921 tcacctcctt aacaattatt tcagcctgtc acactgataa ggaaattata ccaaagaaag
     1981 gaatgagaaa tatgaataca ccacaaattt ttaatttcga gcaaaacgaa gttcgaacta
     2041 ttttagtaaa tgatgaacca tattttgtag gaaaagatgt agcagacgtt ctaggatatt
     2101 cgaatccgca aaaagctatt cgagatcatg tggacttgga ggataagacg cagaacgatt
     2161 cgttcaccgt caatggaaca gcagttgttt tgattaacga gtcaggcctt tacagtttaa
     2221 tcttaaaatc aaaacttccc tctgccaaaa aatttaaacg ttgggtaacg agtgaagtcc
     2281 ttccaacaat tagaaaaaca ggtagctatt caaacgtacc tcaaagtttt gcacaagcat
     2341 tgcgtttagc agcggattta gaagaaaaga accaattact cgaacaacaa attgccgagt
     2401 acgaaccaaa gattagctac ttagatacga ttctttcatc gacagatacg gtagctactt
     2461 ctcaaattgc agctgattac ggaatgtcgg caattgctct aaacaaattg ctaaacgagt
     2521 taggtgttca gcataaagtt agcggacaat ggatacttta ccgaaaacat atgaaccaag
     2581 gatacacaaa atcgcacaca agtgagatac cgaaagccga tggcggcact aaagttgtaa
     2641 tgaataccaa atggacacag aaagggcgag tgtttattta caacttatta atcgcagagg
     2701 gctattaccc tcaaatggat ttagaggaaa ttggttagaa aggagtttta gtatgactga
     2761 cattgcagaa atcactcaac gagatagaga aaaaatcaaa gaatatgtcg aaagttcaaa
     2821 gttcttaact tacaccatgc ttgctgaaag atttggaatt agcaaaagct acttatcttt
     2881 aattttaaac ggtaaaaaga cttctgcaga agcaaacaga attatagatt cgattatcac
     2941 tatgtacgaa ttgtagaggg aggtaaagtc gttggaagat aaaatcattc ttacaaaatc
     3001 agagcttcaa gaaatgttaa acatcgagta tggaacgata atttttagga gggataataa
     3061 gtgaaaaaac caacactttc agagctgata gaagctgctg agaaggcagt aaaaccagac
     3121 gactggtacc gacaaagttt aatcttggag aagttccacg gcatgtcaaa aactacttta
     3181 gttgaatact gcaaggaaat ggaaacaatt cctgaatttg cagaaggaat acttcgtcca
     3241 ggtcactcaa ccacatttat tcattaccat acttttattt ggtttttaaa gtggaaagac
     3301 gcaaacaaat atcgtgtaaa aagtttatcg ccttcagatg ttttgaagga ggcaagttaa
     3361 tgggcaaatt caacagagca ctagtattca gcgcaccgct aatcatctac gctttaggcc
     3421 tctggggaag cagacaagcg ttgataggta cgattgtgta catggtttgg atttttatag
     3481 ggctggatga agctgagtac aaaacaaaaa agccagtcgg gagggactga ctaaaaaagt
     3541 ctaatgtcta taagaaaggt ttgacaataa atgaaagaat tatttttttt aataactttc
     3601 atcatttttt ctatcagtgt tttctacatt aacctttgcg ttgctagatt tgctttgaaa
     3661 agttttagga acagcaaacg aaaatagagc tataaaagat acgaatgtag tagctacgtt
     3721 aaattgctct ttagaaaacg tcaatgtcat tggaatgata attgatatcg atgagaaaat
     3781 ctttgcgata ataactatcc atttttcaat tataaacatt tttaaattaa gcaccattag
     3841 acaaaatgta gttcctatat agaggataaa tgaagataga aataaacata ttagtccagt
     3901 tttaaaaaaa gagaagtaat ctatgtgggc aaattctgtt aatatgcttt tttgatttaa
     3961 cacaatcaaa ctactgttgg acaaaaatga cagtaaggaa agagttgctc cgattactaa
     4021 ataaaaaata gttgttaaca atattatatt atcaattttt ctgctcatgt tttcacccca
     4081 ccagaattat atcaaaaagg agagaagaaa taatgcaaga attagtaatt ttgaaaaata
     4141 aagaagctgt gactacgagc ttacaagttg cagaaagctt cgataaaaag cacagacatg
     4201 ttttgacggc tattgatgaa ctaaaagagg gggttgccga aaattgggca gacctatttt
     4261 gggaagatac ttatgttcat cctcaaaata aacaatcata ccgaattatt tatatgaata
     4321 gagacggctt ctctctatta gcaatgggct tcactggtaa gaaagcttta agttttaaac
     4381 ttcaatatat tgaggccttc aacaagatgg aaaaagaatt aaaagaccaa ttgccatcaa
     4441 ttccagcaac taaacgagaa atggcattac ttgctctagc tgcaaacgaa gaaacgaatg
     4501 aaagagtaga cgctatcgag tctgatttaa acgatttgaa gaacaatcaa cttctagctg
     4561 aaccagatta ccgaacaatt tctaatatgg ttcgcaacaa aattagagtt atttgtaatc
     4621 agcagcattt aaatagcaaa gcaaaagcag aattgtttaa agacttgaat ggcggaatta
     4681 agcgaatcac tggagcagta gctagaaatc gtatcaaggc aaaacagttc gatgatgtta
     4741 tcgattttat taacaattgg atgccttcta cagcgacgat gacaattatt aagcaaatgg
     4801 agttgttaga ggatgaatag agctgaagcg ctaaaaatag gcgtaataat tgctaatcgc
     4861 tggtggagac acaataaacc aaacatccta agccaacaac atattgataa gcaaaaagca
     4921 tggcaacaaa taaaaaagtg actcagccga ccaaagcaat gagtcacaaa gaaaatacat
     4981 ctaaggagat gttaccacat gaaaaaagaa ctttccactc tagatcaata tttgactgat
     5041 cctagttggg gcaaatcgaa tatcaaggaa acaagcaatc gaaaaatcag acgtaatctt
     5101 ttgacggatg aagaactagc atgtgatcaa gacgatttgg gaaattttgt gagtatttgg
     5161 gatcatgtct atcttatcca tttatcgagg aagtccaaaa aaactgaata tatctatgtc
     5221 atcgaagatg gcttgattga tgcgctagaa gagtatgaca gagataactt gattgatatc
     5281 tcttattacg gatcaggtaa gaaatacatt gctgaaatgg aggcagaatt tgatgagtga
     5341 aaaggaacaa cctttgaaaa atagaagtga taatacgctt tttaatacct tatacaaaat
     5401 aaatgtgaaa gatgttactg aaaaacgaaa taacctcact tatctttcgt gggcatgggc
     5461 ttgggcagaa gttagcaaag tgtgtgaggc agtagattat gaaatctatc atgatccaga
     5521 aacatatctg ccgtatgtct ttgataagaa aacaggctat atggttttta ccagtatcac
     5581 agtcaacgga gtaaagcgtg acatgtggtt accagtcatg gatggtgcaa acaaggcaat
     5641 gaaagatgag ccatatacct acgaagtcaa tgattatcag tggaataacg aaacgaagaa
     5701 aaaagagatt gttggaaaaa tcgaaaagcg agttgaagca gcaactatgt ttgatatcaa
     5761 caaaacgatc atgcgctgtc ttgtaaaaaa tctagcaatg tttgggctag ggctatatat
     5821 atttgctggc gaagatatgc cagaagatgt ctcgatgctt gaaccagcta ctcaaagaag
     5881 caaaaagcta ttcttggatg ctttacaact ggttgctaac aagtacgaaa aatcaattga
     5941 tgaagcaatt gttgcattga ctgatgcggc ttctataacc gctgatgaca gtaaatggac
     6001 caagagagac ttgggcattc taaaacgagg cgttaactgg attgaagatc agtacagaga
     6061 agaaacaaaa gagaagtgat atgagtgttt aaaccattga tcgattcata ttcagcggtt
     6121 ctgaaaaagt tcaaaggaaa agacataggt gcaacgatca atgaagaagt gaacattgat
     6181 cgactaaaga cgatgtatga cggatatgat ggcgatcgga ttattgaagt tcgatttatt
     6241 gatccacgtc gatttactgt gcagcaacga aacttcatct atgcgctgat aggcgatatt
     6301 ttcatcgata caggcatgcc aacggacttc tggaaggaat tcttttactt ccgttttgaa
     6361 ggcgtcacag ggcgcaaaat aagcctcaaa gacgaatcga atacaactgt gagtgatgct
     6421 aatgtcttag caaatatcat cttagatttc atctttgaac atcatattcc tttcaaagaa
     6481 ggctatgaga ttttaccagc gaatcaagaa tattacttct acaaatgcat cacaaaaaga
     6541 gtctgctgca tctgtggcaa aacaggagct gacatcgatc actttgacaa agcgctagga
     6601 agacgaaagc gcaaagaagt tgatcattca gagtacacat ttgcagcact ctgcaggatt
     6661 catcacacgg agaaacacaa tataggtgtg atcaatttca aaaataaata tcaaatcaaa
     6721 gggatcaagt taaaccagga aacaattaag aaacttagaa taggagggta aaaaatattg
     6781 tctgacaaca aacgctacta ctatttaaaa ctaaaagaga attttttcga tagtgacgag
     6841 atggttctct tagaaagtat gccagatggc tatatttatt ctaatattct tctcaaactt
     6901 tatttaagaa gtctaaaaca cgaaggtaag ttgatgttta atgacaggat tccatttaac
     6961 tctacaatgc tcgcaactat tacaagacat tctgtaggag tcgtagaaaa agcggtacaa
     7021 attttccgtg atttgcagct tattgacgta ttagataacg gagcaattta tatgtctgat
     7081 atacaaagtt tcattggaaa atcctcaact gaagcagata gaaaaagaga atacagaaag
     7141 aaaatagaag aggcaaaacg gaatttaata actggaggac aagtgtcgga caaatgtcca
     7201 gacaaaacta caccagagtt agagatagag atagagaaag atatagatat agataaagaa
     7261 gaaaagaaag gtaagtattc tgacgaacac ttacgccttg ctaaaaaatt gcaaagtaat
     7321 ttaactgaag attttccaaa agaaatgaac aaagtagata tcgaaaaatg ggcagacaca
     7381 atcaggttga tggaagaaag agataaagcg tctatagaag cgattgagta tgtgatcaat
     7441 tggctaccta caaatgaatt ttggtttgga aatattagaa gtgctaagaa attgagagaa
     7501 aaatttgaga agctcaaatt cgaaatcaaa gcagacaaga ataatcataa aaagcaaagt
     7561 caaaaactac agtacagcaa tcctagtgaa tatgacgact tgccaattta aaaaggagat
     7621 gcatcacatg gaaagcctag caaatgctat ggagaaacta ataagaagag tattagtgca
     7681 aagcgggaaa tgtccagaat gtagcgaacc tttgtatagt tggcgagcta aaaataagga
     7741 tggttcagaa cgttgtaaac caacatgcat gagttgtggt tataaagcgt tacgtgtgaa
     7801 agaggatata cagaccgaac ggatatataa cgacagctta aaagcacgag cgttgagttt
     7861 ttttcaaaat ggttcggtat taacagataa aactttgttt aaatgcaaaa tggagaatta
     7921 tcacgtagtg gaccaagaaa cgaaaattgc tttagaaaga gctaaaagct ttgtaaatga
     7981 tgtcttactg aaccatcctg cacatttcat tctatcaggg aaatcaggaa gcggaaaaag
     8041 ccacttgtca atggcgacag cttgggaaat acttgagcgc tcaaattatg acaagaaaat
     8101 actttttata agctatcaag agttattaga gcaaataaag ttttcttata acaatgctga
     8161 actgagaaaa gaaattgaag gatcgcttat agccgatatt aaaacaactg atttggtggt
     8221 ttttgacgat attggagctg aattaggtag tggggtatca aatagtaggc agtttacaaa
     8281 caacacgtta aacacgctct tagaagcaag acagaacaag gcaacgatca tcacaacaaa
     8341 cttatctggt cctgaactaa gagaagccta cggtgaaaga attgtttcta ggatatttaa
     8401 gaattcagaa ggttatgcgc tgaaattcca gcaaacagca gacaagcgca taaaaccagt
     8461 gaaaggtagt atcgcatgaa taaataccgt aataaaaaaa ctgttcatcg aggtatcaag
     8521 tttgattcta tcgcagaagc agagtattac gatctagcct tgtggcaagc tgaagcgaat
     8581 ggctggaaag taaaacttca ggaaagattt gagctgatgc cgaaatttga actagacgga
     8641 aagaagtatc gcaagatcga gtatattccc gacttcacat tttataaaaa cggcaaactt
     8701 gtcaaagtcg tagatgttaa aggaatgcag acaaaagact ttaagatcaa ggcaaagctg
     8761 ttttgtcatc aatatcaagt gccgttgata ttagctaaaa aatatcggaa tacgttcaag
     8821 gaagagcgtt tttaaagagg tggtctatca tgacaacaga agaagtgatt caaatgcgaa
     8881 ttcgaagcat tcaacgtgag attgacgatc tggaacgaac aaaggcagtg atggtcaatg
     8941 aaacggcgag aaaggcaatc gatttgcaca tagagaactt aagaagggaa attcgtagat
     9001 tggaggaatg agcgtggata agaaagcagc aatgaaacga atcatcgaac tgacacattc
     9061 tgagaattgg caagaagaca aagaaatagt tgcagaagtc caaaagctcg gtaaatcaat
     9121 gtggactgaa aaaaccagac ggagaatgcc gagaaaaatt gcaatctggc acggtgaccg
     9181 aattctagta acaggtaccg ctgaacagtt atctgaaatt actggtctga gcaaaaacat
     9241 catctgggat agagcgaaga atatggatat tgattctaaa ggaagacagt ttaggtatgt
     9301 ggaggagaaa taatgaatct cattacacaa tacagtgata tcatcctcaa gaaaatcatg
     9361 atgaagattc agaaagacaa aaaatcaaaa gaacgagcgg aattagttaa gttggaaatg
     9421 gctgaaacag gggcaggagt gcgaagtagc aggcattgga aggcagcagc aaacattgaa
     9481 ttttattaca acgaaattca aaaagggttc gatcaaatgc gtgagctgga tcggcaaaca
     9541 aattggagca agaaacttca tcaagatcgt ttcaaatttg tagagaaata taaagagata
     9601 ttagacgaat acatggaggg cagcaaatga taccgagatt tcgatcgtgg tacacaccat
     9661 ttaaaggtaa aacaattgga caagaaatga aatatgggca agcaggaagg ttgatcactc
     9721 atgctgaaat ggctccagat aaatatgtgc ttatgcaatc cacaggactg aaagataaga
     9781 atggcgtaga aatatttgaa ggggatgtag ttctggttaa tgtaagtaat ggttttgatc
     9841 atttagttaa tgaaaaaacg gttgtacaag aatccgagtt tcattccgga ttagtttgta
     9901 aatcattaat tagtggaatg gaatatagaa tattcagcca ccaaaagatg ggatacgaat
     9961 atgaagtcac cggaaatata cacgagaata gcgagttatt ggaggtagag tgattatctt
    10021 agctactgac tacatcgatt ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa
    10081 ttgtggaatt aaaagtaaga atagaatcat tttgttacgg atatcggctg atttagcaaa
    10141 atatctttat ctgagcaaga cagcagtata ttacactgcg gctacacgtt atcagtacaa
    10201 gggaaaaaca atcagtcagg attattatga tcgtttaatc agtcctgaca tacatggaaa
    10261 atcaaaatca gcgatcaagt tatttggcgc aatagagata tatgccgatg attttccaaa
    10321 tttatggttg aaggaggaac agtaatgaaa gactggttag aagaagcaag cgccttagtt
    10381 ggctgtttca taaactgtgc gtttatatct gtaattattg cactggtggg atttgtatca
    10441 ttgaaagtga ttatattgct ttggcagtta attttttagg aggaacagcg atgaataaac
    10501 aggaagcaat caaaaagtta gaaagtatta aagtgatagg aaatgatgca ctagctgctt
    10561 gctataacga gagtataaat gcaggtatta cgttaatgaa aaaaatagac gaaccgaaga
    10621 agctagttat tccacaactc gtggccggtt ggcttgagaa atctacggac ccttttacga
    10681 aagctgaaaa aatagcgtat ttaatcaaat ctaaagatgg tgattcatat tatttctgtg
    10741 attggtttgt acgagatggc atagtgacgc aagaacaagg agaagaatta ctcgcttggg
    10801 caacgagaca atcatatgaa acactattga gcttgtacaa cggctacgag gtcgagaaag
    10861 agccgttgta cagtgtaata attgctggcg agtaccttgt caaggagatc agttgcagta
    10921 atgaagttaa actagtatca acagcttacc tgccacaata ccttgcacac cactaccaac
    10981 tgaccgaaaa acagatcaaa gcaattgatg aaagatactg gccatttgct gtgccagtgg
    11041 aagaggtggc ggaaggatga gtagacagat aatgctccat ataccagacg gaacaaaggc
    11101 aatgacgatt accattgttg ctgcacaaaa agacggtggg ttggcgttga aaacaaaaag
    11161 cattgatacc aaacagattt tagaaggcag agatgtcgag atagagatcg atgaggagga
    11221 agcggaatga atgttcaagt ttatttaaaa agagatttgg ccaggtttcc tcgaagctgg
    11281 gatacaaatt cccacccatc cgaggcagaa gaagagggct atctaatgtc tgctaacatg
    11341 atagaaatta caagagatga tgcggatgag tttgataaaa aaagatcatg gtactcggtt
    11401 ccgaatccaa tgtatacggc tgtaatagaa gattatcata tatccgacag atttattctg
    11461 attgatctag aaaggcctaa gaaacgaatt aaacagtata gacgttgtgg attttcaaca
    11521 aagaaatggt gataacaaaa cagaggtgaa gaaaaatgaa caacagacac cgcagagtag
    11581 caaaactaag aaatcaagaa ctgaatgtag cgaaagtaaa gttcgaacgg gagtatggtg
    11641 tttctgcaga agaaacatat aaagtggcaa gtcagtgtgt tgctgatgca agtgatgcta
    11701 ttcgtaagtt tggaatttcg atactagata ttaaatggga ggatacagaa tgaagattta
    11761 tgtagtaaag tttggaaatc aattttacag aagcgatgaa cgttctatag gggctaacac
    11821 attatccatt gtagacatac tccaaagcgc aagatggttt gataatctcg aagaagctaa
    11881 ccaagtttca cgacgacttg gtggattaac gcaagtatat gaactggtca ctgtcgatca
    11941 tgagggggta gagtaatgaa actaaaagac ggattttacg ctagtagtca tggtatcggc
    12001 ggtttgatgc tagatatgcc gacaaagaac cctaaaacac gtgagaaacc aaaattcaaa
    12061 gtcggtgaca tggttcgctg tgaagcagaa ggatttatct atccatttcg tggatatgta
    12121 gaaaaggtat tgtcgaactc agcaatcatt cgtattgaaa acacgatgga atgtgacaag
    12181 tggttagcga aaagcaaaga gaatttagct gtagcgagat tagttgacat ggaagtaatc
    12241 gagagcaaat aaaaaagccg gatcgctccg actaacataa taaaacagac aagtttatta
    12301 tatcacataa aggagcggtt tgacttgatg caattgttac gagaggtaga tttcaaacag
    12361 acaagatgta atgcgagaga tgtgctgaag aactttcggc gtttggagcg gatggcaggt
    12421 cgctctttga tagatattaa gtctccaatt attacggata tgccgaaggc gccgaagcac
    12481 ggcaataagg cagaggacgc gatcattcag atgatggata tagaagcaga gagagatgcg
    12541 attttagcgg ctttgatggc tcttagtctg attagccgtc agatactcta ctacagcttt
    12601 tgtgtgccag atagcttctc aaactacaga attagccgtg aagtgggtta ttcagaaaga
    12661 agtatacaac ggatgaagtc ggaagctcta atagagtttg cagaagcata taaacacgga
    12721 agaataattg cttataaata atttggcggt tttttggcgg aatgatggcg gtttttagct
    12781 atttaccagt gatattatgg tagtgtcgaa agattagtga taggtctgag acaaaataat
    12841 aataaaagga acatcgtttt attattgttt cacaattaag cttcgataga cagcaacgga
    12901 aatattaaga ataaggatgt gaatttcaac tccttctaaa ttgttcttat tatctatcat
    12961 ccgttgctgt ctattgtttt tttaattatt cacacgataa ctaaaggtga gtgaagagaa
    13021 atgattccat taataatttc aatttttgcg ctctgtctta atgtctatat gattggattt
    13081 aaaaatgggc aaaataaaaa atagtagcag ccaagaataa ttttatagtg tcactgtggc
    13141 ggaaagggta gacgcttaaa aataaggtca atacgtcgag ggatagcctt aacgttttat
    13201 gatttgacca tgcaatgttc gattcattgc cagtgacttt ggcaaccgag ggttggaaat
    13261 gggcgctcaa agtacacgag caaggcgagg tcgatagtaa tcgatggaat cggtgtaggt
    13321 tgcttgatag agctataact gcatctcgtt tatgaggtgt agtttttaca gattagatca
    13381 ctctttgagt ggtcttttta ttttacacaa aggaggtaac aagaatgtat agaccacaat
    13441 acttagaaca gaagtatgaa gtaatcactg tgcaaaacgg taacggtgag atagtacgaa
    13501 agtatagaag accaataaag agcgatacat acaaacgaaa ggaaagcaat gaagttattc
    13561 cattgtatgg caaaagaata gctaagcatt aaataagatt gcgaaaggag acggaatatg
    13621 accgaggaat tctatagatg gctattacag ttgataagag aagatcgttt agttaagttc
    13681 tatcagtctc ctaaatggcg caggcttaga gagaaagcga tgaaacgaga tcactatgaa
    13741 tgccaagagt gtagaagact aggtaagtat catagagtag aaaacgttca tcatataaag
    13801 gaagtcaagg atagacctga cttagcttta gatttagata atcttatttg tttatgtgtt
    13861 gaacatcata atgaagttca tggcagatat cttacagcat tagataaaca agagaagaag
    13921 atagaaagct ttgctaactt cgatgcaagt gaaaggtggt aagtgcatga tcatcaatga
    13981 taatggcaga gagtatgata cagaaaagat tgaagagtat tcatcttata ctcagggatt
    14041 aattaaacgt ttgatatacg ttcgctatgt agatattagg gatctgttat cagataactg
    14101 ttgtagtaaa tacaaagtga atcaagtaag agaagcgttg aataaagata ataacgtcga
    14161 aagaataaaa aatgtttttg gatatagtat tgaagagatt aattattaca ttgacttcgc
    14221 tgaagctttc attccgatgg tgagataacc cccccttaaa ataaatcgca aattttttgg
    14281 gggtgatgaa acggaggggg ctgtcaggaa aagagatttt ttcgaacttt atcatgaaag
    14341 gagggctaaa atgtttaaaa acgaattgtc tcaaaatcgc tacagagaaa aattacgccg
    14401 ctctttaata agccaattgg aaagtcagaa aacaaatatt gagccattct tagataatgt
    14461 tgatcgttat atcagtttat gggaaacggc gatatcactg gaagaagata tatccgagaa
    14521 cggcattaga ctggagaatg gtaaaaagaa tgaatcagta gcgttgcttg tttctgtcaa
    14581 caaacaaatg ggattgatgt tggataaact tgccattact cctgaattgg taggtgaagc
    14641 aaatgaatca attcctgagt tataagcata ttgaaaattg gttcaaagct atagaagaag
    14701 gcactatcaa ggtatgcaaa gagcaattat tgctaaaaaa ttatctagaa gaaagagtct
    14761 ttactagaga agatatttac ttcgataagc agatggtaga ggattcaatc aatataccag
    14821 cacaatactt tccattcgaa ttaattccgt gggaaaaatt tctacaatgt tttatttatg
    14881 gtgttcgatg gaaaaaagat aaaacactag tgttcaatag atatctttca ttaatgggac
    14941 gtggtaatgg taaaactggt tttgcttctt ggaacaactt ctttctacta accgctaaac
    15001 acggtattaa aaattatgat attgatatct atgccaataa tgaaagccaa gcaaagacta
    15061 gttttgatga tgtatttaaa gtaattaaag atcatcctga tttagataaa aaagtattta
    15121 aagctacgaa ggaagttatt caaaatatcg ctacaaacag caaacttcgt tataacacgg
    15181 caaatgctag aacaaaagat gggaagcgac caggtgcaaa ccgctttgat gaaattcacg
    15241 aaaatgaaga ttattcaatg ataaatgtgg ctacttctgg tggtggtaaa attcgagatt
    15301 atagagaatt ttatgataca actaatggtc atgttcgtgg tggtccgctt gatgacatta
    15361 tagaagaatc aaaaatgatt ctttctggag aacttggaat tgacaaggat ggagcagaat
    15421 tttctagttt gtttccattt atttgtcgct tggataacga taatgaagtt gatgatcccg
    15481 acatgtggga aaaagcttgt ccaactatta attacaatgc agatttaaaa cggaaaatgt
    15541 ttcaagaata ctctcaaatg caacgtaatg ctggtttaag acttacgttc atgaccaaac
    15601 gaatgaacag acctatggaa gatacacgat ttgctgttgc ttcatatgat gatgttctgc
    15661 atacgaaaga aaaagaattt cctgaaaaaa tggatgaagt gataggaaca gtcgattttg
    15721 ctgatagacg agattttgcc agcgttgggt tgctaggaaa atacgataaa gatgtttatt
    15781 ttacacaaca tacttttatc cacgaatcag cccttcgatt acaaaacatc aaacgagagg
    15841 ttatagatat ttctatagat caaggaaaat cacagatcgt tcatggaaaa aatatagaag
    15901 ctgattatat tgtaggttgg tttcttgaaa tgagtaataa atattatatt aaaaaaatcg
    15961 ctatggatat gtaccgtgca aaaatattga agcccgcttt agaagaagca ggttttactg
    16021 tggaaattgt tcgaagcgga tctgttacac atggtatgtt aaaagatctg gttgatgacc
    16081 tttttattaa tcaacgttta ttttttggtg acgatgcgat tatgcgttgg tattgcatga
    16141 atgtatatga agagcatatt tctaatggaa atatacgcta tgaaaaaata gaacctgaaa
    16201 ctagaaaaac ggatggcttt ttttcattcc ttcatggttt gaatttttta gatgatattt
    16261 atgattctgc tcctgtaaca gtcacaaata gctcagtaga aaatacagga actggattta
    16321 ctcctctagt attctaactt gaaaggaggt gagaaagtgg ggatttttca aaaggcggta
    16381 ggatacttca caaaaaaagc aacggttact ttagaagaat acttttgtaa attgcaagtt
    16441 gattttgtgt atcgaaaatt tgcgattgaa acttgtattg atttgattgc aaatgcgatg
    16501 agtaaagcgg aattcaagtc atatgaagat ggaaaaaata aaaagaatga tctttactat
    16561 aggctgaatg tagctcctaa taagaaaaat aatgcaacag aatttagaaa aaaactgatc
    16621 aggagattaa tattctacaa tgaagtattg atcgtttctc cgtctaataa ttctagcgaa
    16681 atatttattg cggatagttg ggatgtcaca gaatatgcat tgaaagatga tgtgttttct
    16741 caagtgcaaa ttaacaacat agtccttgat agagaatttc tagaaagtga tgttatctat
    16801 ataaaatacg cagatcaaca aattaggcaa ctagtcgatg cgtattatca agcgtatggg
    16861 aaactcattt ctagtgccat gaatgtttac aagcgctcta acgctcgtag atacgtactg
    16921 aaagggaatt tattccgacc gcaagacaat acaacacaag atcaaatcaa taaaatgatg
    16981 acatcacaat ttaaggcttt tatggaagct gataatgcag gtgcggtatt tcaattacaa
    17041 aatgagtaca cattagaaga tttcagcgga aacttccaaa gcaattcaag agatataaaa
    17101 aacttaatag acgacatctt tgagatgaca gcagcagcgt ttcacgttcc gaaaaaccta
    17161 ctaaagggag acatgagtgg gttatcggat caagtggacg cttttttaat gttcgaaatc
    17221 ataccgattg ctgaacttat tcaggatgcg tttaacgcta gtctctatga agtagaagaa
    17281 tacttgtcag ggaattttgt acgtgtggat acaactatga tcaagattac tagcttcaaa
    17341 gatttggttg acgctattga tgtaggcatt agaaatggag tatttacaat caacgaagga
    17401 agagaacgcg ttggaaatga tcgctctgat aaggcgatgg cagatgaaat atttataact
    17461 aaaaacaatc aacaagtatc gaaaggaggt gaggcgaatg acgacaatga aaacatttct
    17521 agcagtaaag aatgaaggcg cagtaccgca aatttttatt cagggattta ttggttctag
    17581 ttggttcttt gaagggaata ctgacaaggg aatcaaaaat attttggata gtctaggtga
    17641 tcaagaagaa attgaagtag taattaattc aaacggtgga gacgtatttc aagggattgc
    17701 tattgggaac ttacttaagt caaataaagc aaaagttaac gttgtgatta acggcttagc
    17761 cgctagtgct gcttcaatta tcgcaatggc tggcgatact ataaaaatct acaacaatgc
    17821 acaattgatg attcaccgcg cttccacata cggagaagga aatgtcgatg acttccgtac
    17881 gattgctgac caactggaat caattgataa atcggtaaag gcttcatata aaacacgatt
    17941 caatggcaca gatgaagcat tgcaagaact tcttgaaaaa gaatcgttta tggatgcaga
    18001 aacagctttg agttatggat tggtcgatga aattatcgat gcagaaaata gctcaggtac
    18061 tgaagctaaa aaagaacaaa gcgttgaaga aattttgaat gacgttaaag aaaaaagagc
    18121 agaaaaaatt gctgcattta cagcagcatt aaataaaaca tttggacaag gagatgtaaa
    18181 ataatgacag ttaaaaattt aaaaggtgta acagctgcaa gcgaccaatt gatgaaagct
    18241 tttaaagatg gtaacgaaga atcttttagc gcagctatgg taagcttatc taaggaaatt
    18301 caggataaaa ttttagaaga agcaacagca aaaaatcaag atcaattagt attaatgaac
    18361 cgtggtcagc gtgtattaac tacacaagaa acaaaattct ataacgaagt agtgaaaaac
    18421 gaaggttttg caggggtcga agaattagtg ccagctactg tatttgaacg tgtatttgaa
    18481 gatttagaac aatctcatcc actattgcaa aaaattactt ttgttaacac aactggtgta
    18541 acagaatgga ttgtgtcacg tggagtcaat ccagcatggt ggggtaaact ttgcgaagct
    18601 gttaaaaaag ttttagataa tggctttgat gtaattaaca tgaagcagtt caagctatca
    18661 ggttatattc ctgtatgtaa ggcaatgctt gatttaggtc cagtatggtt agatcgttat
    18721 gtccgtactg ttttagtaga atcattgaga attgcattag aacaagcaat tgttgatggt
    18781 actggtaaag atatgccagt cggaatgatg cgtgacatga gcaaacaaac tagcggagaa
    18841 tatgctgaaa aaaaagcaga acctattaca gctttagatg ctgtaactat gggcggtttg
    18901 atggcgcgac tatcaaaatt caatatcgaa ggtgtgaatg atccgattta tcgtaatgtg
    18961 aatccttctg atgtggtcct aattgtgaat ccaactgatt actggtctaa agtattccca
    19021 gctaagactg tactaactgc taatggagaa tacgtacaag tattgccagt accagtttca
    19081 gatttgcagt caacggctgt gccagaagga aaagcagtta ttggggtagc ctcagattac
    19141 ttcatgggtg taggatctac actaaaaatt gaagcttcag atgaatacca ttttgttgaa
    19201 gacgaacgca tttatctagc taaacaatat gcaaacggtc aacctaaacg taacgatagt
    19261 ttcattgtat tagatattag cgctttggga actactacta caactacaaa accaacaacc
    19321 acaacaacta caacacaagc gtaggtgatc agaatgaagt atattctttg tcagccggca
    19381 atcaatcggt ttaaatggga gcttgaagtt tgtttaacta atctgaagaa actaggaatc
    19441 aaagatatcg tattgctttt cagcagacac gatgatcaga ttcctatttt ttttgagaag
    19501 gaatatggtg ttgaagttca tgtgtacgat gatctgcggg acgacaaaga gtatattcct
    19561 tcgattaaac catatttatg gtggaaatat ttagaagaag atcattcgcg tgaggacgac
    19621 cgatatttct atatcgattc ggatgtcatt ttcaataaaa gaattaattt gcgcaaattg
    19681 ccttctaaag atgatgtttg gtattgtagc gactgctgta gttatctaag tcttgattat
    19741 attagaagct gtgaaaacgg agaaaatatt ctaaaagata tggcaaacat tgtaaatgtt
    19801 acagtagaat ctttggaaac tataaacact aattcaggag gcgcacagtg ggttattaac
    19861 cgccctaaag cgaattattg gaaaaaggtt tatctggatt ctaatcggct atatcgctac
    19921 cttagagggc aaaaaacaaa tataaaaatc tggacagccg agatgtgggc acagctttgg
    19981 aacatgatgt atttcaatat tggtcctaaa gttcacgagg aattagactt ttgttttgct
    20041 actgatccaa tagaaaaagt taaagaagta aaaatcttac acaatgctgg agtaacaaca
    20101 aacgatgaag atttattttt caaagggaga tacgtgactt ctacgccttt tgatgaagat
    20161 ttatcatttg taaacaagaa aaaatgctct tacgcatatg ttaaagcaat taaggcggtg
    20221 gttagatgac gcctgaacaa gtgactgaag aattgctaat agctgtgaag gataatattt
    20281 acgttacctg gaacgaagaa gatgagtcaa ttaaaaagat gatagctaaa aatgctgttt
    20341 atcttcaaag taaagtgagt acaacacttt ctttttctcc tgaaagctta gaatacggat
    20401 tgctaatcga aagatgtaga tacgactgga atcgtgcttt agatgagttt gaacaaaatt
    20461 tcgctagtga gttattaggt ttcattcaac attatgcgct acaagaatat attgcaggtg
    20521 atgtgaatgg cgaataatcg tagactcgaa gaaacattca acgatggttg gttaaagatt
    20581 ttgacgcaaa ctaccaaaag aaatgaacta ggaaaaaaga ttggtgtaga agatacagaa
    20641 atcacttctt taaaatttag aaatctttcc atgagagata gtgatataac agctatggat
    20701 gcgatgggat cgaaattaac taagaaagta aagactccat ttcatccaat cgccaagaaa
    20761 tttaataaag atcaatattt tatcgtaatc gatagtatgc gttacaacgt tatctatgcc
    20821 gattacgata atttttatat ctatttttat cttgaaagtg tgggtgaata tggtgattga
    20881 taattctaaa gaaaaagaac gtttaaataa gcaaatttct gctatcaaaa cttccttaga
    20941 agagcatttt ggcctcaaac tctttcaaga ctccgtaggc gaggatgagc tacctgatga
    21001 ttttaattac ttcattctcg aaacaggaga aatagaaatg atcactgagc caaaatatag
    21061 cgtgggtcaa aatctatatc taactttcta ttcagaaaat agagaagatt taacaggaga
    21121 ttcactagat attatttcat tgattcaaaa tcgttcgatt cgttttcaga gaatggatcc
    21181 caatcattta aaactagaga accaagatcg ctatatcgat caattggtat ttacgtttag
    21241 acgattattg aagagtgatt gtcatggcta aaaatagttg ggagctaaaa ataaatggac
    21301 atgatgaact tcttgtgcgg atggaacgct attcaagcga gagcgaacga ctgattaacg
    21361 aagcattgaa atcaaaaggt tcggctattg cagtggatag gattacagaa aaaattcctg
    21421 tttctgaagc agatttaaga agagggcacc aacacgcaaa aaatagtcgt ccacttaaga
    21481 ctcaatacat taatttgggt ttcatcatta gacctacaag aaaatttgag tatttaaaat
    21541 atcctgattt ggggataggt acttctaaaa gaaatcagcc ggacgaattt atgagaagag
    21601 gattaggtct tgcacttgat ccaattacag aacttctgat tcgtcaattc gataaattaa
    21661 ataaatagga ggaacaacaa tggctaaaac aacaacagta acaacttttg acaatataag
    21721 tattaaaaga atttctttta attttaagaa tgcaacaaat gcaatttcaa ctgattgtaa
    21781 tggacaatta gatggcgaaa cagaaatgca gagaattgta aaaaaatgcg gttcaacaga
    21841 agtaaaatcg aaatctaaac caatcaatat gacggtaaca attactgcac atgtaccgat
    21901 ggaagtttat cgacgcttca atgggttgaa acaagatgaa cgtattaaat cgggaattta
    21961 ctcttatggt cctgattccg taggggaaga tttctctctt gctgcagaga tcgtggatga
    22021 tttcgaagaa aaaaataagc tgattggtat gttagcatgc acttcgaata caggattaac
    22081 attctctatt gaaaatggtg cggatgaagt agctgcgtta gaactagaaa caaaagttat
    22141 gcaagatgaa tttggtaaat tttatcatga agcaattgtt gcagaacttg aagaagactt
    22201 aacagatcaa tggatgacga atctatctgc tgatgtgatt aaaaaaactt cagttgtgac
    22261 aactacggcc actcaatcac agtaaaaaaa acggaggtag cgaaatgaac gaagattact
    22321 caaaaattga actaaacgat ggaacaattt tgaatttaga acctaaactg aatatcaaga
    22381 aattattgat gatcaataga gattttaaca cagacgagtt tgcaaaaatg tcgatgggaa
    22441 aaggctctat ggatattaca gttattcaag gtgcaaaagc cgtatatgta gcttatcgtc
    22501 aagcgaacat ggtcgattac atttcattcg atgaatttat agataaatga gattttgata
    22561 tggaggttgc agtagctgta tacagtacta tgatgttcaa acaagcacgt gatgcctatc
    22621 aaaaagaatt cgaaaaagca aataaggaaa aaaagcttca aaagtaaaaa tgccaaagct
    22681 cttagttgaa acgtgggtcg atgtctattc gatgttgacc gacgtttttt ctatgccttc
    22741 agatttggtt ttaagcgata tctgtttaga tgacattttg caaatggctt acaacaagag
    22801 cgcttatgaa ggatggaaga actatgcaat aaatcaatcc cagaaaaact aaaggaaagg
    22861 aggtaaaaaa tggctaaaaa gagaacggaa gcagaagtaa ctttcatagc taacgatgac
    22921 ggattgaaat ctacgttaaa agaaatcagt gctgaattaa ctaaaaatag agcagaatta
    22981 aaactagaac aagctcaatt gcaacagact ggttctgaat cagacaagtt aggaagtaaa
    23041 ttatcttctt tagaaaagca gtatgaatta caaagtcaaa aagttgaagt aactagccaa
    23101 cgtttagcca atgcgaaaaa atattatgga gaaaattcca ccgaagttca gaaacttgaa
    23161 agagaactga ttaatcaaca aacagcgcaa caacgtttgt caaacgaaat tgataaaacg
    23221 agtaatgcac tagctcaagc aaaaggcgaa atacagacgt acgagtctac aatgcaacag
    23281 ttggatagcg aacaaaaaaa tgttcaagct agtgcttctc tgattgaatc cgaatacaaa
    23341 aaatggcaag caactgctgg tcaatcagct tctgaagccg agaaattagc gaaagcccaa
    23401 gaatatgttt ctcaacaatc tgaaaatgcg gagaaaacga tagatatcct aagacgacag
    23461 ttagaagcta cacagtctga atttggcgct acatccacag aagcaatgca gatggaagcg
    23521 aagcttaatg atgctgaacg tgaatttgaa gagttaggac aagctgctaa aaatgtagat
    23581 acaactaact tggacgatat cggaagcaaa atagatatga ataatttaat ggaagcttct
    23641 gacgttttaa gcgacattgg cgataagctt acagaattag ggaaacaagc agtggactct
    23701 gctaacagtg taggtagttc ccagagtaag atacaagcta attttggttt gtctaaacaa
    23761 gaggctgaag aattaacgaa tgtagccaga gacatttatt ataaaggttt tggagaatcg
    23821 ttagatcagt ccacagatgc attgattttg gtaaagcgta atttaggaga tttaaataat
    23881 caagatttac aaaatatcac ggaacaagct atggtcctag aaaacaccat gggcgctgat
    23941 atggatgaaa cgttacgtgg tgtaaatggc ttaatggtca atttcggctt gagtgctcaa
    24001 gatgcaatgg atttaatggt ttcgggtact caaaacggtt tagataaaac gcacgaatta
    24061 ggcgacaata tggcagaata tagccaatta tggagtcaaa tgggatattc agctgatgaa
    24121 acgttcggaa tgcttcaaaa tggtttagat gcgggtgctt ataaccttga taaagtcaat
    24181 gacttagtta aggaaatggg aatatcgtta acagatggtc gatttgagca aaacatggat
    24241 atgtttagtg aaagtactag aaaagctttt gaagagtgga aaaatggcgg aggaacacaa
    24301 aaagacgtta ttaattccat gattcaagat tttagcaata tggatggtca atacgaccaa
    24361 ttaaataaag cttcgacaat ttggtctgcg cttggcgaag ataatgcgat gaaagttgtc
    24421 caatctttaa ctgatgttaa ccatacattt gatgatgtta gtggatctgc acaaaaaatg
    24481 aatgaagatt ctactactcc gttacaagaa ttaaacggaa aaatagctga attaaaggat
    24541 tcattagctc ctataggcaa cacaatcata gatgcactcg aaccagtaat tgattttcta
    24601 ggaaagatgg ctgatgcgtt taataatctt ccacaaccag tacaggatta tgccgtagcg
    24661 attggcggat tgactgctgc atttacttta ttaatgccaa taatagttgg cttcatggct
    24721 ctaggtggtc ctactacatt aataatagga gcagttatta ctgctattgc tggagttata
    24781 gcaattataa aaaactgggg cgcaattact gactggttta agggaatatg gagtaaattc
    24841 actgattggt tgggtggtac ttgggaaagt ataaaagaag gtgcctcatc agtttgggat
    24901 ggagttaaag aaacctggtc tggatttgta gattgggttc aagatatttg gcaaggagtt
    24961 tctgattggt ttggagagct atggagcgga ttagttgaag gagcttccaa catctggcaa
    25021 ggagtccaag aaacttggca aacattcgtt gattgggttt caaatatttg gaacggagtc
    25081 aaagaagtat ggtcgattat ttgggcagac attgtaggaa ttgttcaaat accatggaca
    25141 ttaataacgt cattgattca agctggtatt aatattatcg tgggtatttt tgatgtagct
    25201 ggacagttat taggcgcagc ttggcaagct gtttggacac ctatttctga tttccttaaa
    25261 aatacttggg atactatgac acaatggata agcatcgctt ggaacggaat tgtaactaca
    25321 ttccatacta tatttgatcc agtagtggca tggtggaatg gtatatggac atctattagt
    25381 actacggctt caaatatttg gaattcaatt agtgcaacag cttctagtat ttggaacagt
    25441 atcaagaata caatcactag cttggtacaa gcagctgcta cagtaattca aaatatttgg
    25501 tcaactgtat ctagttggtt aggtggaatt tggaattcaa tcagctctac agcatcaaat
    25561 atctggaata gtgtgactag tagtataagc aatgctataa acgcagctaa aagtgccatt
    25621 caaagtgttt ggaatagtat atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac
    25681 actgctttga atctttggaa tggaattaca agcactatta gctctaaagt aaacgatgga
    25741 aaaaatgcaa tttcaagcgg ttggtccaat ctaacaggta ttgtttccga catattcaat
    25801 aatgttaaaa gtacaattgc taacatttgg gaaggtatca aaaagactgt tagcgctccg
    25861 attgattgga ttagagataa aatcagtggc atctttgata atttgaatat ttcgatacca
    25921 catattccgt taccacattt taaattgagt ggggaattca atccattgaa gggaaaaatc
    25981 ccaacgttgg gtgttgattg gtatgcgaaa ggtagtgtgt ttaattctcc gaatattatc
    26041 ggtgtcggtg aagcaggacc tgaagcagtt ttacctttga aaagatctgt tttgcaagaa
    26101 attggtgatc gtatcttgag tagcacatca gtttcatcta gggcacaaac gattcaacct
    26161 gtgaataact acgaattcaa tttcacaatt gatggtaacg cagatgaggt tactatgaag
    26221 caaacaactc aacaaatcat tgatagcatt acaaaaattc aaaatgataa tgcttcggca
    26281 tggcgttaaa caggagagta tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa
    26341 tgactgattg tatacattct ataatcgatg gatttcctga ttatttgcat aaattggctt
    26401 tagctgaaag accaaccata ccttctccaa aaagacagag agttgaaact tctgttttag
    26461 gtaggttagg tggcttagta caagattact cgtttgaaga tatgtcgttt acattgcact
    26521 ataactattt agaggatgtg gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc
    26581 gtcattggtt aaactatgca aagaaattag aattctctga cgatcccaac gtctattacg
    26641 ttatccagac tatcgatatt ggggatgcag aaaacgatat tgttgaatgg ggagagttcg
    26701 atgtaaatat tactgcgaaa ccattcgcaa gagttcaaga agatgtacct ataaccgtag
    26761 ataaaccaca gtcatttaac ttgctgaata atagtttaga agaaagtttt ccaaagatta
    26821 tcatcactcc ttcagctact tcatgccagt tcatcttaaa tgattatgtg tttagtttcg
    26881 aaggcttagt agcaggaact gatgtagtca ttgatagtga tttgatgctt tgctacgaag
    26941 agcaatcgga cggagatatt ttagatcggt ccaacaaaat gaagaccatg caatatccaa
    27001 cgttgcaagt ggatattaat cattttaatt gtactggttt gagcaaaata caaatttatc
    27061 gtaacgggtt aaggtaggtg aaatagatga tcgataattt aataactatt tacgataaaa
    27121 atgacgcgaa taatttagct gaacatttat atgatacgca aggtttaggc gctttgtcag
    27181 actggttaac agctactgtt agcaataaac taaacggagc cgagatattt cagggtactt
    27241 attcaataaa cggaactaat gcagatttga ttgtagaagg acgtattatt cagtgttatg
    27301 tagatgaaaa tcgagcaaaa cagcgtctac ggatttatta tgcaaaaact tctgtaatag
    27361 gcaatacgat agaagtaaaa gctgaaccta ttttcaatga tataagaaaa tcggtgttga
    27421 ataaatatga cagcggaaca gaaaagatca ctgctactca ggcatggcaa aacgcaaaag
    27481 ttttagcgaa accagctatc ccttcgcagt tttctttctc gtcattagta gatacgcttg
    27541 ctaatgtgaa gatagaaaag gcgaattttt tagaattctt tggtggaaaa gagggatcta
    27601 ttctagatcg atttcatggt gagtttctaa aagataataa cacattacgt catgaaaaaa
    27661 gtctaggtac ggatcataaa atcaaagcga tttatactaa aaacttaact ggtcttgact
    27721 tagagattga tgctcaaagt gttttagttg gagtttatcc attcattagc agctcttcag
    27781 aaggagaaga cgagatcact ctaccagaag aagttatttt cacggattac gtggatgatt
    27841 atcctgctgg atatgtttct tttgttgatt ttaaagacaa agcgactgat gtagccacat
    27901 taagggaagc tgctaaagac tggttgaaaa caaacataga taaacaaaaa ccacaagtga
    27961 gtggttcgat tgaattagta ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc
    28021 tagaaaaagt ttcgatgggt gacggagtag atgtgtatca tccacagtta aaagtgaata
    28081 tgtcagcgag aattgtggaa tatacgttta atgttctaac caattcatac gataaattag
    28141 ttgtaggaaa cgtcaaaaca aacttcttag aaaacacaga gaataatgtc agcaatttga
    28201 ttaatgatgc cattgatcaa ttgaaaaatg gtggcgaaat cagtgattta atcaatgata
    28261 ttgtagatca tcaaactgat atgattactg gtcaaaatgg tggttacgtt ttattagatc
    28321 ctaaagaagc ccctagtcgt attttgatta tggacacacc agataagaat actgcacgga
    28381 acgttttgca aatcaacaac gctggtattg gtttctctaa aactggcatt aatggaacgt
    28441 atgacaccgc atggacgtta gatggcggat tcaatgcctc gtttattaca gctggtgaga
    28501 tagtagggat tactattaga ggtactacat taattagtga tggcactgat tatagaacaa
    28561 gtatagctaa tggcaaaatg acttggtact caaaaaaagt taacaaagat attatggagc
    28621 tagaagcacg tgattatgta agtgctgatg caggtattgt atcatacacc atgaaaactg
    28681 gtggtggttt catgattaga aatccacagg ggaatttggt ttttagtacg tgggataatg
    28741 gtaataacaa accgttttta tcttttggtg cgcccaattt caggtatagc aatgctagtt
    28801 atataactga tggcgatggt agttctttaa gcattaatgg tagtgcgggc agttcatggg
    28861 agtttaaagt agctggtagg actatgaaat ttactagtga tggtatgcta acgttgccag
    28921 gttgtttttt tggttcatgg gaagatggaa aacttgctag gtttgaacaa tcaacggtac
    28981 aagtatataa aaattttact gttagaggta ctaaaaactc aaccgtacca acagaacatt
    29041 atggacaacg actattaaac gcttatgaaa ctccagaata ttatttcgct gattatgggg
    29101 aagccgttac aggtgatgat ggtaaagttc gtgttgatat tgatcctatg tttgccgaga
    29161 cagtaaatct aagtcgatat atgacacatg tgacacctac agagctagtt ttgtgtgctg
    29221 ttactcatga agatattgac catttcatca ttgaaactag taagccaaac gtattggtta
    29281 gatggaattt agtggcacat cgactagggt atgaagatgt tagattaaaa gaggatacag
    29341 catatgatag cacagtgctt gaccaaaaac gtttttaaaa cgaagacaag gaggtatata
    29401 aatggctagc agtttatata atttggcttt agatttcagc aaagaattaa actacaccaa
    29461 agctattatg gctcgtcaag gtgacaaagg aattacggtg actgttaaac cgtttttaaa
    29521 tggcttgcag atggatacga gtggcggaac ctttacatta aaaggaacaa caccatctaa
    29581 ccgttacgta gatagtgtcg ctactagtat aactagcgaa gaagtcacat tttctcttga
    29641 tggaacattt atgagtgaag caggatacta taaacactgc tacgtagaat atagaaaaga
    29701 taatcaaatt ttaacgacgc aagatatcat ttttttctca ctaggagtgt ctgacatttc
    29761 gcaaggccaa gccgatgaat atgtttcgca attagaagaa ttgattcgaa agtataacga
    29821 aacttttgat gcttttctgg ctgaaattaa aggtagaatg gatagcttaa ataaacagat
    29881 tactgattta actggtcaag ctaaaacact acaagacaag ttagatgctc tgaaagaaga
    29941 aatttctaaa ttaggtaact tacatgtgat gtactccaac agcatcgact tcgggggcta
    30001 tgattatagt gggaatccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg
    30061 ttaccacggg tcactaacta atgttaacgg aatgttacat tttatgtctg atggtacagg
    30121 ggacattgat atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac
    30181 tctgagtgcg aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt
    30241 ggtgtatcgt acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac
    30301 aacagatgat gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat
    30361 cacaaattta gatcgatttt atattagtgt tagtttttct aacagggata aaataaatgg
    30421 tggatttaaa ttgtacgaca tcaaaatcga agaaggctca acagccactc cgtatcagcc
    30481 aaacttacta gatgaccctt actgggtagg taaaacatcg ttgggtaaga atattgcaaa
    30541 taaaggtacg acgttcccaa ttaaatctag cgcgtacgaa atatataaag gtaacacgga
    30601 agaagagctt ataataggtc aaacgtatac tatcacgctt aaaggaacaa aacccgcaag
    30661 tcaaaccttt gtagcatata attattggaa cgttaatttt ggagatctaa aaccagttga
    30721 gggattgaca gacgtatggt ctctaacatt cacaccaacg aaacttgaac cgggtttgcc
    30781 taaagacctt cgcatttttc agtcgcctaa agaaacggcc ggcgcatgcc gaattgactg
    30841 gctcaagatc gaaaaaggcg acacacgaac cccgaatatt gagcaatata aataccgagg
    30901 aatcggcatg cgagactcaa acaatccaaa agattatgta tgggatctag aacctagcta
    30961 tgttgagaat aacctagcta cagatatcaa ggttgctgaa atcacaggta aagcaaacaa
    31021 ttataccgat gggaaagtat cggagattaa ttcgcagttg actgcttcaa ttaatgaagt
    31081 agacaccaca gctaaggatg ctcaaacaaa agcgaatgct aatgcgactg ctatagatga
    31141 attagacaat aagatcgatg aacgcattaa tgatacagct actaccacat taacagttac
    31201 aaacgggaat accggatcag caaagcttta tcgtgaagga aaaacagttt ctatatattt
    31261 tgtggcttta aacggaaaac gcagtggtgg aaatgattca acgatactaa caattccaga
    31321 aggctatcgg ccaccaatta gttttgagca actggttggc tcgatagacc gttctacttt
    31381 gaacagtgct cagttatcta ttggtgcaga tggagccatt aaatggcgaa gaaactcaag
    31441 ttatggatcg gattatacct ttgcaattac ttacacgatt tagaaaagcg tgaatcgata
    31501 tgaaggcagc atatagacca attgaacctt acggattcga gcaaatcatt gtgaatgatg
    31561 aagaacattt accggaagaa tgcacagaag tcgaaccacc gattccaaat tggaaaccga
    31621 aattcaatta ctgggaggga aataaatgaa aaacatttgg aaatatggac gtactggcgg
    31681 agagtacgca ggaaaagtat tggacgacat gcttgtatcc gttccttaca cagatcagcc
    31741 accgcttgaa ggaattcgtg ctgatggcga accgttaaca atcgctgatc aaatgtttga
    31801 tcctaaattg aatcaatgga ttattttagc gaacgcacta gatcacaacg atttaaacaa
    31861 tctcaaagca atgtatgagt cgttagaaaa tgagaacggc gatttaaaac agatcaatgc
    31921 caaactcatg ctaagcgatg tagcaattaa acaggaaaat actgcattga aagaaaaagc
    31981 ggatagttta gcacaaatca attcaaaaat gatgcttgct tcgttacaaa atagcaaaga
    32041 catttcagaa attaaagagc aactaaatcc agcttcaaag ggaggtgagt agtatgttta
    32101 gttttagcga tgtgaaaatg atgtatgatt ggggctgttt cactaacgaa caagtaatgg
    32161 ttttcgttcc gttgtgcatt actgaagaaa aagcagataa aatcattagc aaagaagaga
    32221 gcgcatctta attgatgtgc tttttatttt gattcaagga gttgtcacat gattaattta
    32281 ggggaatggg gagcgatagc aggatcaata accgctatcg tttctttgat tttattagta
    32341 ataaaaccaa ttactgcatc tttctcgaag attactgaga ctctttcaaa agtaagtcac
    32401 aatttagatt tgctgactaa agatttagaa tcgagcaaat cagatcgatt gatgattcat
    32461 gaagaactaa agaaacacga tgaaagatta gatacacatg cagaaaaatt ggtagaacac
    32521 acgcaacaaa ttaaaacttt
//
LOCUS       DAZULG010000018        32346 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000018 DAZULG010000000
VERSION     DAZULG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.018"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..330)
                     /locus_tag="ACG9CY_000792"
     CDS             complement(<1..330)
                     /locus_tag="ACG9CY_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937325.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKC"
     gene            complement(337..885)
                     /locus_tag="ACG9CY_000793"
     CDS             complement(337..885)
                     /locus_tag="ACG9CY_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS4937326.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(930..1262)
                     /locus_tag="ACG9CY_000794"
     CDS             complement(930..1262)
                     /locus_tag="ACG9CY_000794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937327.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1276..1533)
                     /locus_tag="ACG9CY_000795"
     CDS             complement(1276..1533)
                     /locus_tag="ACG9CY_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937328.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1728..2270)
                     /locus_tag="ACG9CY_000796"
     CDS             complement(1728..2270)
                     /locus_tag="ACG9CY_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFS4937329.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2291..3382)
                     /locus_tag="ACG9CY_000797"
     CDS             complement(2291..3382)
                     /locus_tag="ACG9CY_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4937330.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3390..5600)
                     /locus_tag="ACG9CY_000798"
     CDS             complement(3390..5600)
                     /locus_tag="ACG9CY_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4937331.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5616..8144)
                     /locus_tag="ACG9CY_000799"
     CDS             complement(5616..8144)
                     /locus_tag="ACG9CY_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4937332.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8196..8606)
                     /locus_tag="ACG9CY_000800"
     CDS             complement(8196..8606)
                     /locus_tag="ACG9CY_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS4937333.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8619..8837)
                     /locus_tag="ACG9CY_000801"
     CDS             complement(8619..8837)
                     /locus_tag="ACG9CY_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFS4937334.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8834..9826)
                     /locus_tag="ACG9CY_000802"
     CDS             complement(8834..9826)
                     /locus_tag="ACG9CY_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4937335.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9905..10816)
                     /locus_tag="ACG9CY_000803"
     CDS             complement(9905..10816)
                     /locus_tag="ACG9CY_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4937336.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10835..11179)
                     /locus_tag="ACG9CY_000804"
     CDS             complement(10835..11179)
                     /locus_tag="ACG9CY_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937337.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11199..11477)
                     /locus_tag="ACG9CY_000805"
     CDS             complement(11199..11477)
                     /locus_tag="ACG9CY_000805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937338.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11836..12336)
                     /locus_tag="ACG9CY_000806"
     CDS             complement(11836..12336)
                     /locus_tag="ACG9CY_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937339.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12337..12882)
                     /locus_tag="ACG9CY_000807"
     CDS             complement(12337..12882)
                     /locus_tag="ACG9CY_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937340.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12879..14135)
                     /locus_tag="ACG9CY_000808"
     CDS             complement(12879..14135)
                     /locus_tag="ACG9CY_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4937341.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14496..14807)
                     /locus_tag="ACG9CY_000809"
     CDS             complement(14496..14807)
                     /locus_tag="ACG9CY_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937342.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14830..16305)
                     /locus_tag="ACG9CY_000810"
     CDS             complement(14830..16305)
                     /locus_tag="ACG9CY_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFS4937343.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16754..17206)
                     /locus_tag="ACG9CY_000811"
     CDS             complement(16754..17206)
                     /locus_tag="ACG9CY_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937344.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17208..17573)
                     /locus_tag="ACG9CY_000812"
     CDS             complement(17208..17573)
                     /locus_tag="ACG9CY_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937345.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17672..21955)
                     /locus_tag="ACG9CY_000813"
     CDS             complement(17672..21955)
                     /locus_tag="ACG9CY_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFS4937346.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22102..22341)
                     /locus_tag="ACG9CY_000814"
     CDS             complement(22102..22341)
                     /locus_tag="ACG9CY_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937347.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22431..22673)
                     /locus_tag="ACG9CY_000815"
                     /pseudo
     CDS             complement(22431..22673)
                     /locus_tag="ACG9CY_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22786..23022)
                     /locus_tag="ACG9CY_000816"
     CDS             complement(22786..23022)
                     /locus_tag="ACG9CY_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937348.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(24033..24943)
                     /locus_tag="ACG9CY_000817"
                     /pseudo
     CDS             complement(24033..24943)
                     /locus_tag="ACG9CY_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(25139..26974)
                     /gene="lepA"
                     /locus_tag="ACG9CY_000818"
     CDS             complement(25139..26974)
                     /gene="lepA"
                     /locus_tag="ACG9CY_000818"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS4937349.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            27121..28341
                     /locus_tag="ACG9CY_000819"
     CDS             27121..28341
                     /locus_tag="ACG9CY_000819"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4937350.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28361..28855
                     /locus_tag="ACG9CY_000820"
     CDS             28361..28855
                     /locus_tag="ACG9CY_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4937351.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            28833..29519
                     /locus_tag="ACG9CY_000821"
     CDS             28833..29519
                     /locus_tag="ACG9CY_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937352.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(29670..32279)
                     /gene="clpB"
                     /locus_tag="ACG9CY_000822"
     CDS             complement(29670..32279)
                     /gene="clpB"
                     /locus_tag="ACG9CY_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS4937353.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
BASE COUNT         9414 a         6871 c         5001 g        11060 t
ORIGIN      
        1 acatttaatt tttaaacaaa attctcccca aaaaggaact ttttctagtg aaaacttggt
       61 aatctctttg taattaattc ttgttaaaga ataattaaac acttcatttt tacttgttat
      121 tttttcattg actataacta gttcatctgg taaaaaaatt aaccaacaaa catcgacaaa
      181 cagtaaatga tataatccat ttccacctcc aagcatattc aatactggtg aacatgcctt
      241 agaaacacga atatagttat ggtttaaata gatattttct ttaaacattg cttgtttaaa
      301 agccatatgc tcttgttcta aacttttcat aaacttttac tctaatgcac agctagcagc
      361 agctgctgca attcctgcta caatatttac cttagctaac tctttagctg cagcttttcc
      421 taattgtttt tctgcaattt ttaataataa atccgcagct ttttcaaaat taaaagcttt
      481 taatgcttca ataaaatctc cctgcgtacc tatagcccaa gccactcctc ctccaggaag
      541 agaattaatg gctacacatc cagcatatcg tttccaatca actttctcca ttctaggaac
      601 aataatatgt tcataaaaga ctcttgctgc tcttccttgt tcgttatctt gactgattcg
      661 ttcacgaata gcaccaaaat cctttatatg gtatacacca tgttcgtcta aaaaaccaac
      721 tgtatcaaaa taaaaagtta attcatctac taatttattc tttatttcta aatcaactgt
      781 attcactttt tctgtggcat acacacttgt tccagtacta acaaaagtac ttaaacctaa
      841 taagaaaaca cctaaaacac caaatatttt aattattctt ttcataattt ctcctccata
      901 aaaaataagt tactttttaa atatcttgtt tatgaattca ttccccaaag gccttttaag
      961 attgatacag cagtccgttc acttctttta gacttgtcat acaagtcttt taaatgatcc
     1021 catatcagac tagtttgtct atctagcttc aatccattaa acgctaatga acggttcatt
     1081 ttttctatta ctttagataa agctacagaa atttttatac cttcacaacg cgcatcttgg
     1141 atttcaataa tagtatcatc aattagtttt tgaatttctt gcctatcaac atattcaatc
     1201 ttagaaagag aatcttttaa agctactaat gattcaacga tttctttttt ctcttcagac
     1261 attttcttcc tccttctagt gacccgtaaa gccatgtaga gcagagtcca ttgaaccagt
     1321 aattacacta tctacaacgc cattccaatt tactctacat tttccatttt tcagatttgt
     1381 cggtccatac ggatagcctt tttgacattc aatgttcgga ttataccaga taggattata
     1441 ccattcagcc gcagaagcag attcagtcga tacaaacaat cccacaccaa caaatccaat
     1501 acacatcaca ctatagccaa ttagtttttt catctcgtat ccatctccta aaaaatagaa
     1561 tagttcttat agttattcat aagacatatt ttttccgttt ttgttttatc ctttctgtcc
     1621 tataacaact tttttcaagt aaagaatgac atagtttttc ttaaaaaaac agtctgtttt
     1681 tttctatttc ttcggtgtat aataaatatg agtgttcatt ttttatttta atacgcactc
     1741 cgtacataat aaatcttgat cgcatcaatc ttttgagctt cttgatctac tttcaattga
     1801 agtaatacaa attgagtaac cttattcatt gtttcttctc caaataacaa atattgatcc
     1861 ggattctcta tatcttgtgt cacctggtaa agtttcccag ttgttttcat ttgtacatgc
     1921 gggtctacat caatactatt gtctttaata caacgatctg tcatatagtc cctcacagct
     1981 tgattgcgtt tataaacatc tgtgaaatta ctccattttc tagcaaaatc ttttaagaaa
     2041 ttttttgtca tttcatctat ttgcatttgt tgcgctgtct cgtcttctaa tgaaccaatt
     2101 tgactgttac tttcagtact ttgaagagtt ccacttgttt cttttactgc ttcgattgtt
     2161 tcttctgtcg ttctcaattg tgttttttgt ttttcagaag aaaaccaatg acaaccagta
     2221 agacctatca aacataacca actaaatact aaaattatcc cttttttcac gtttctagtc
     2281 actgcctttc ttatttcacc ttacgaacca gactacgaat ttttgtttga ttgtacgtgc
     2341 gttgataccg agccataatc cgtccttgct cggcattctg ttccacacta gagaaatgtt
     2401 gtccgccata tgaaacatct gttataattc cggtgtgacc atagattcca ggagacaatg
     2461 caccacctgc ttgccaattc accacatctc ctgcttgtaa atcacttggt tttgggttca
     2521 tgattacttg ccaaccatag gttgtccagt tataatcttc ccctattaac atcgcaaaca
     2581 tgtgtccaga acccattaac tgtgggccac ctaattgaga tacataccat gccgtaccac
     2641 cataacattc cccattattt acggatcgtc caatgatccg atccaaaacc ttcgcattgc
     2701 ctttactttt tcctgcaata agcgaagttt tttttgttgg taggctattc caatgcttga
     2761 tcgcaaaagt atcatattcc gttaagttat aggacgttat gatactcatt gctcctcgat
     2821 aatacggact cccttcggta atacctgctg ccgcatagcc tccacgacct agaatcaata
     2881 aacaacttcc aggatcttta ttgcccacac attgattaaa gttgctttgg ctcgccacca
     2941 taaagccata atagtcataa caagctttta agttcggaaa cttcatatag aaaccacctt
     3001 cagaaccacc aattccacgt gcagaacctt gtgggtaggg acaaccagga aaccaggtaa
     3061 tcccaaaaaa gttcgtatcg tttcttccag attgtgactg tccataagct gattcatagc
     3121 ctaattgaga aagaatcaca ctcggtaata actgatactt tacagctgca ccaactgctg
     3181 cttgcgcatg ttcatctgtg attggaccac gtcctttaat ctctgaaaaa atcggtaatc
     3241 cttcggtata ttccccatta aaagcgacag ctagacctgt agcttctcct ccatgctcag
     3301 aaagttctcc tgaaaaaaag aatggcaata gaaaaaaggg aagaataaag aaaagaagac
     3361 aacctagaca ccctttcttc atttcttcct catcctttct tcgataatgg tttttggact
     3421 gttcgtcctt ttggtaaagt aaaatcagtt cctggttttg cgtccaaaat cggcagtgtt
     3481 ttaggatcct tttctatttt ttccgattgc tctgttggct tatctaaaga agaaaatgca
     3541 ggttgtaacg attttggtgc ggaaatcgac ggtttacttt cagacgaacg ttgccccggt
     3601 tgttgcacag aacgatttcc tgatagagtg gctaccgacg aattaggaac agagtattgg
     3661 ttcaataact gctgcattgc tggataactc ccatttttcg cctgattttt cacttggcga
     3721 atctgttgtg aaagagaacc agcagctgtt aattgtggca cgggtacttt tccagaaggc
     3781 aacggaatcg attgaccaac aaaagcgata cctttattta acaatggttt tctttgatta
     3841 ttcccttgtt gcttactcaa ttgtgccaat gattgtacct gttgttttgg tcttactgaa
     3901 ttcatggacg aatttggaat caaaccattt gaaagaccag aattccactc tgttttcgat
     3961 ttggtagaag acatttcagg ctctacccca tgataacgaa catgacgacc actataaggc
     4021 aaggcttgat ttgaacgccc acctagttca aagggattaa ccacttgcct cccttttacc
     4081 gattcttgtg gtgtacgttt ttgttgaaac ttttgaaatc catgtatcgc tgcagaagcg
     4141 agtggattcg ttgctcgata cacattccct gctaatcctg cacctttgat tgccaaattt
     4201 tgtccagcag ttgatagttg accaggcagt tgtttcattt gagacaaaga atgacttgct
     4261 atgtttttcg tgttgtttat agtttgatct gcagctccta accctagttt ctttaataaa
     4321 tctttccgtt ttacaatgag taaaattgat aggagtgctt tgacaaacat atttcctaaa
     4381 aaaccaaaaa cagtattcat tggaattaaa tcgtctacta tgttatagat caaaaatgcc
     4441 accaaaataa tcacactgta aaaagatttt tgaaacatca agcccaaaaa ggactttaag
     4501 gtattcatga gcatataatc aaatactgga acaaaagaag cgagtaatga aaaaggaatc
     4561 acaagtaata aacacaacac caacatttga aagataaaac gaatcatgcc aatcactaca
     4621 agtggtaatc caatacttag attaagcaat ggcaccatag aaccaacgac tgctttataa
     4681 acagctttag ttggttgaat atagacacga taagccttct ttttccctct atcttctcct
     4741 gcagcagtgg tcacttttgt ctcaatatca gataattctt cacttgacgc ttccttggct
     4801 agaaattctt gtggttcaat ctctgcttga tacaattctt ctaaatttgt tgtcccataa
     4861 tttaacaata tatacggatc aacaacagcc gctttataat aaagttggcg cactttttga
     4921 atggcttcct tttgtgtcgt tggcactaaa acaccattga catcttttgt cgccaattca
     4981 tctagactat ttgtagcaga aaaaataaag ccttctacct ccacggacaa atcatttaac
     5041 cctttggtaa aagcttctcc tttactaggt acgttactac catagccaaa ccaaaccatt
     5101 gaaaaaatga caacgaagag aaatcgaata aacaccattt ttgcgtaatg tttccctcta
     5161 aatatacgaa gagtaaatag atataacaca tacaagacga caatccccgc accaatagga
     5221 tcgtatattt ttgaataaat agtatccgaa aaagtgaaaa actggtgaat taactcgttc
     5281 attgcagctc cttcatagag cttctcaatg acaaaatcaa ggccttgcca aatcaattta
     5341 cttaaactaa agaacatatt cgtcatcatt tttagaaaat ctgtgccatt tgccccaaat
     5401 aacattttgc ctaatgaaaa ttcactatca gataaataag aagaaaaggt atcaatatga
     5461 aatttcccaa tctcatagat cgattgatcc acctgctttt ctgccgcata caccaatcca
     5521 ccaacaaaaa gcagtccgaa tccaatcgtt aaaaaaaaca aaacgcacca tttcttttcc
     5581 ttatgcttta tataagccat tgtttcacct catttctatt gcatgattaa cgtatcctgt
     5641 tcttctactt gagagtgact tgttttttcc gttgtttgta atgcagcggt catttcttca
     5701 aacaaactgt gtacattgag ttttcctaca cgtccatagg gatctaacat ccaacactgt
     5761 cctttaatca tgtggctcat ttcagctaag ttcgcttcat ttacttccaa acgtaaatga
     5821 ttcaaaatct tttctcgctc actgtcttca tcaaaagcaa agacgacacc aaaattccct
     5881 ttttctttat ctgaacctaa gtcaccaata aattgtgtat ctaacactaa ttgattcatc
     5941 tgggaacgcc ccacacgtgc cattgctgca ataatactac gtcctgcgtt cgaattatta
     6001 aatacccatg cttctgtaaa ataaatcgct gttttcttac tactatctcg ttttccaaac
     6061 atttcacaaa aacgacctaa actaatcatc aaacacaaag actttcgatc cgcatctgta
     6121 tatagttctg gtcggacatt gtcatttggt aatttcaaat cttggatctc taaaatcgtt
     6181 gttttggcat taaagtctaa tcccgggttt tctccataag aaaatcctaa acgtaaaatc
     6241 gaatcttcta ccgtcaagcg taaaaactca ccatattctc gaacctcttt ttgttccatt
     6301 tgttctaaat agtcgaccac atgcatcaag ccaacttgtt gtccggcttc tttttgaact
     6361 aaaacttctt ggattccctt taataccgcc ccttttaaaa ggtgattggt acctaacgga
     6421 cttaactcat caatcatatc ctgggctact tgtttcgcat ctactcggga taaacaaaca
     6481 attggatcta atacaccata attacgggga tttgtagcat ctaacgtgac ataactcatt
     6541 ttttgcaaat gttctacaaa caaagggtaa tgtttctgat aatacgggtc ttgaatgagt
     6601 ttcgtaaacc atttgcgctt ttctcctttg ggatccacaa ataaactttg tacattcata
     6661 aacgaactat acaaaaagag caagccaact aaaaaggtct tccctttacc ggtttttcca
     6721 gttactgcta catgaggcgc atcaaacaac gcacctttga ttccttccgc aatggctaga
     6781 agattcaaaa agacaaaacg atttgacgca taaattaatt cttctaatgg tgttttttct
     6841 cctcgagaac caccatcccc taatgtatct tgccgaccaa tataccagcc ggattccata
     6901 cctaattctg atactgctga aaacaaccat tcgccaactg cttctacagt tgttcggtgc
     6961 ttccaatgta actgccgtcc caatggttgc cctaataaac atttatagaa caattctcgt
     7021 tgatcatttt ttgcacggtg gatatggata atcgtatgaa gttgttgaat aaccgttgca
     7081 ctattttgac gaacttcttc aaccgtttga ccgtatacaa taaaacatgc gatccaatcg
     7141 acgacaggtt gcccattatc tactcgttgc tttaaatgtt ttactaatcc tcgaacagtt
     7201 aataagcgtt cactaggttc ttcatcattt tgaaccaatt ccttatgctc tgttttgaac
     7261 cgttccgata gccaattcag ttttaaacca atacctccct gtcctttcaa gcgtggatag
     7321 tgaattttca ttcgcaattc tgctgggaaa gaaagatcct gagcaaattg aaatagatca
     7381 caatttctca tattttcagg tgtttcccca ataggcaaca atgctaaata actactccct
     7441 aactcatctt ctaattgaag aaggccttta tgttccttcg gtatacgtac atacgttcct
     7501 tgattgtctt tcagtacttc ccaattattt tgttcttcta ctttgtgagc cattcctcga
     7561 atatattgca gacgattgag atacaacagc tcttttttta ctaagggacg aatagaaaag
     7621 ctataaagta attgtgtcac ttcttttaca taaggatcac tttcagaaaa caaagtttcc
     7681 aaatcatcta cttctaatcc aaataagcgt gctaaacttc ctgctaaatc taaccagatt
     7741 agatttatta catcatgaaa actctccacc gttgcttttt ctttgacttt gattcctaaa
     7801 ataaatgaag gacgatagac ttgaccttct cgttccaaca atgtcactgt ttcttttgca
     7861 taatacgcac caattgcttg actatcggga gaaaatccct ttcctaatgt gtcaaacttc
     7921 gtccgtaaat tcattgttcg tggatacatc accaactcca catcttcata atctgctaaa
     7981 agcaacagta aacgagccat catttctttt atttcctgtg tcttttttgg tgtgtttttt
     8041 gaaattgttc gtggaaaaac atgatagtag gcccagatac aatcctctcc atttaacaaa
     8101 caatttttct tgaatgcttt tactggttct tttacaagca ccaatgaatt tcacctccca
     8161 ataaaaaagg aacaaccagt tgattgtccc tttttctaat cctctaaata aacatactta
     8221 ttcacaaaaa gtaattcttc caatgcattg tttgatacct tgtcccagtt ataatacaca
     8281 caatcaccat tcatcatacg aatcatataa caaaaccaat cccataaata aaaatgcact
     8341 ttttttccat caggttttag ttttgccagt gctaacacac ataaataggg gagtcctata
     8401 taaaaaacaa ctcctgctgg tggaaaaaaa gaaatgaccc aaccaataaa tgaacgtaaa
     8461 aataaatatt caaagattac gaccaacaaa aaagttgctg cattgactaa tgcaactgga
     8521 aatggaagac gccaattgct tgtaatctgt tgtacgcgat aaggttccgc ataaacaatc
     8581 gaataatcat aaggttccca atccacttca ctatcctcct aatccataaa taatttttca
     8641 atgattttac caaacgcatc aaatacacgt gttggatcat ttaagaagaa ataaactgcg
     8701 gcacctgctg cgatagatat taatgcattt cctacttttg atttcataaa atttcgcaat
     8761 cccagataaa caaccaccat tattaaaaaa tagccacctt gcgttaatat aaaatctaca
     8821 attcccttca gtgtcatttc cgttcttctc ctttcctatc caaccaatta tgtgttaatt
     8881 tttcaacata atactgattt gtttttttcg taatgacaat cgtcatttgt tctttttgta
     8941 aaagtgacgt atcaccatct cgaaagacga ccgttgcttt tacaataaaa ggttttttcg
     9001 tttctttata acgcaatact ttcatctcta cttgatcaac tttagctgtt tgattgagtc
     9061 cttcaggttc tttcatcatg tatgacatat cttttgcatt gtttttcgca tacttatcta
     9121 agaaatcttc cacaaatcct tcaacatctt tcgcttccga cgcatcaacc gtttcgtata
     9181 atgtgtcatc ataagtaatc attcgttgtt tttctgtatt ctttggtagt gccgtaaaat
     9241 aaggataagc aataatacga cagcccttgt ttgaaataga aaagggaaga ttgagtactt
     9301 gtgtgattgt cacacttgtt tctttcttac tctctggatt catcgagcga atatcataag
     9361 ttactttata actagcagtt ggaatggttt caatttcttt taatccaata aacgaagcag
     9421 cagttaattt tcgaacacct gaaacacttc ctggttcttg aataaagttt tgtgcataaa
     9481 actctttttt taatgtttct tgacgttgtt gttcttgttc atgattatcg aatgtattca
     9541 tatacaaagc aacaaaacga ttcataaagg attcaatttc aggtgtgtac gttaattctg
     9601 accgactttg tacttctttc ttttgtaatt gttggatttc atctttcaaa aacgacactt
     9661 gaaccttcgt tttcatggat tgaaaaaatg ccaagccccc tgaaaaacaa agaaataaaa
     9721 ccaaacacca aatccatttc actgcttttt ttgaatggat tttcttttgc ttcggtactt
     9781 tctctgtata aactttttct ttccgccgta gttgtcgttt tttcataagc tactccttct
     9841 agtatgtttt tactagtacg tacgattgac aacgataagc ttgtttttct gaaaaatagt
     9901 ccattcaatt accctcattt tctattcctt ttctctttct tttatatcca acaaatccag
     9961 caatacttgt caacactcct cctagaacag aaagtgcaac atgtatttct tcaccagtct
    10021 ttggtaagac ttttgttttt ccatctgctg ttttgatttc aatattgcca cttggaagtt
    10081 tctttacagg aatcgacaaa tcctttacca aattcccttc actatcttgg attaatggaa
    10141 taccatcttt tacaccaaca attgtttgat cacctgctac ttttaatggg gtggttggtg
    10201 ttggaatatg ggtcaattcc tctacattac tagtgacaat cggtacactc attccttgag
    10261 aagtattcgt cgtaatttca ccgcttgggg ttaccgatac tgtgacttct tttgctggaa
    10321 caataggttt attttctttt ggaattgctt ctgatttttc aacaggttgt ttcggttctt
    10381 ctttttgttc ttcactggta gacgacatcg ttggctgttc cgtacttgac gaatcagtat
    10441 tcttttcaga agaactggtt gttcctgatt gttccggagt tgtgactata cttgaatccg
    10501 ttgttgttga actatgcaca ccttcttctt cgatcgaatc agtcggtaaa gttgattcag
    10561 gaaactctgg ttctggtgtc actggctctt tttcaggttt ctctgactgc aattgttcag
    10621 gttctgctgg tttttccgtt gttcctccat ttagctctgt ctcaacatct ggtaatcctt
    10681 cccctggcaa tgtttctaca atttctgact taggtgtttc tacagtttca tctgcataaa
    10741 ccaatgaact ccctaattga aacgaaaata atgaaattcc tagtgttgtt acgactgcta
    10801 atgttgtttt tttcatgatt attcctccaa ttttttatgc aattcgtgtt ttgttcatag
    10861 acgccgcact aatattatct gaacgataaa agaactcccg tccgttttct tctaacccaa
    10921 catatggatt atttttgaat aattgaatca atagttcact ggtgttgtat tttggatagt
    10981 ttgcaaaaaa ataatactgt gcttgtcgcc catccgaaaa ttcaacaagt aattcataac
    11041 gataccgctt caatcctttt cgttgtttta aaggatctct ataccaactt gtccatcgtt
    11101 ccattcgtgg atagagatag acacgataca catagaacaa tgccatacca cataccacca
    11161 aaaataataa tcgccacata aaaattcccc cttttcttct acctttcttg aatcatcgaa
    11221 cattcatacc ctctatagag atttgaaaaa tccatcatca attgttcata taaacgttgt
    11281 tgcaaatagc gattgtttcg aatacataat tcttcatagc catcaatcac ttgatcttct
    11341 ttttcatagc atcttgtagc tctatgacgc tcagcttgca agacataacc aaactctgca
    11401 ccatatcgtt ctactgttaa taactgttga ctacgcaata atgcaatata acaatcacaa
    11461 aaatgttcaa ccgacatttg atttttctca cgtttgtgaa ataggtattc tttagaaagc
    11521 acattattcg ttaaacaatc tgataaatag cgataatctg gcggtcggtt attttcatct
    11581 gtgaattttt cccataataa actgattttt attcgttcag cggctagata gtcttgttcg
    11641 cgtggataaa attcatcaat cgcacaaagt tttttatcac ctgaataaac aaatccatat
    11701 aatgcgaaac gaactcttcc ttttcaattg gctctcctaa aaattccagt gacataccat
    11761 cttctccttc ctaaaaaaaa taaaaaagca gaaaccgatt tggcttctac ttttctttcc
    11821 ttttatagct cttttttacg gaagaatccg caaatgacat tttttcaaaa aatcaatcgg
    11881 cacaagttca cgaaaacttg gagcaatttt ggatagaatc acttccccat cttgtaaaat
    11941 acaatcataa aaatcatcag ttacttgata cttacgcttc ttacaataat tgataaaata
    12001 gtagcgatca ttttctattt ccattcgttg ttctactaag cgcatacgaa catcatcata
    12061 gaaataagcc acaaatcgcc cttcaagttc atgtttttca atgggatact cttctggtaa
    12121 caatctattt tttcgtcgtt tatacattgc tggatacctt agaagaagtt catctaaacg
    12181 tctgtcagta tatggtaact gacgaatcaa cgattctaat cgttctttat acagatagaa
    12241 taattggcgc aagcaatagt gtaccgcctc tggattttct aatactgttg gatcacgtga
    12301 ccaatacgca ccaacataaa tcataatttt tttcatttag acaatcacac cagcaatact
    12361 aatgaccgat ccacaatagg tagaacaagt ggcatagcga ccaatttcct ctaattctat
    12421 ctcaatctct tctaaagaaa gatatgtctg ttcttctgtt ggctggcgac tcatttccca
    12481 agtgacagtc accaatgaaa tttcttccat aagaggtttt tcgctgctaa gtgctgccac
    12541 acagttttca tatatcttca ttttatcgca gcctttttta accgctaaaa tcgcaggtat
    12601 tggcaatgaa aatcgataaa agcgaatgaa ttctagcctt cctttccttc ggctaaattc
    12661 caactcttcg agttcttgtt cgtatccctt acgaacttct aataagcgtg caatttcttg
    12721 ccagttctct ttttcctgat aacgtccagc ttttaccgct tgttcaatat aaggaactcg
    12781 aaaacgaaaa taagacaaat caaccatctt ttcatttaat gcttcgattg cagcctttaa
    12841 atccaacagg cgcagccatt ctgttgtcgt atatgcattc atgcaatttt ccctcctgac
    12901 gttcccatcc attctagata gttttgttgc tcgtcattta gttcggcatt atcaatccct
    12961 tcttgtacaa agtctcttcc taaaatacga tctgcttctt gtaacaactt taatgtcgga
    13021 gctacttgtt tcaaaaacca gcgttctgaa cgatctaatg agaatttttc tggctttact
    13081 gtaaaatcca attcttccat cgaacgaaat aattttgccc atcgtttatc aacaatccgt
    13141 gcatcacttt ccagtctatc ccaaacaata agttttgcat taattaaacc aacgccaatc
    13201 tctcctaaac gtttcggtga atcacctaaa ataaatgctt ctactgcttg tgttgctttc
    13261 tcattggcta accgtaactc atatcgatta taaataccat atttcgttaa tgcttctaat
    13321 tctgaaattt tttctcggtt cgcaatttca aaacgtttct cataaaaatt aaaaaagaga
    13381 ggactctttc gcgatccaaa atacaaagat aatccttcac ttcgaaaata gccatcatct
    13441 aaaaaacaac cgccaatatt ctggaatctc ttaaagtcca tttctaataa acctgctttt
    13501 tgtttttcta gtaattcaaa gagatcgaaa tgttcttccg tttccaatcc ccaaagttca
    13561 tctaacgcaa tatcgaatcg gctacattgc acacgtgtat aaccagcttc caactttcct
    13621 tgatttttcc aaatggaata tagccatgtt cgccaatctg tttgttgttc acgcatgact
    13681 tcctcatatt cccggcaacc ttgtcctgaa aattgcacaa gtacattttc tgttacagtt
    13741 cgttttgaat gccagaacac ccagatattc ccacgaacag aaagacagtc aaaaagatac
    13801 aagcccgttg gacgtgtttc aaattctgtc agatccattc gtaatacacg ttgaatcact
    13861 gttttgtagt ctgtcccaaa aaaggaaaga cgcaaataat caatcattgc tcgaagatct
    13921 attccgcttt tcatatacaa tcgaaagcgt tccttgattc gttgttcaaa atcctctggg
    13981 atttttcttt tcccttgttc catctttcta atgtaatctt ttgagcagcc catcaattgc
    14041 gcaaatcttg cttgtgatag cggcttttta tctccactta tattatgaat tgcacttaac
    14101 ttctttctgt agctttttag ctcaatcggt gtcattttct cgctcctttt ataaaataac
    14161 aaaatcgatt ttttttaacc ctttagaaac accgatatat caactagtcc ccctcgtttt
    14221 ttgggaaaag tagtacaact gctgacctta gataatcact tgatacaaaa gggtttataa
    14281 aacttttttc tatgtgttat gttatttctg ccccccatat tatatatcgg ggggctttac
    14341 acaaagaggc cgtgcgcaag cgcacgacct ctttgtgagc tgcacacgct gctagcgagt
    14401 gcgcattata ggcccatgct tcgcagcctt aatgcgcacc gccaggcaga gtgcggctac
    14461 cagactacgt ctagagccct cccgatagat aataactaca aaattaaatg atctaatttt
    14521 gttatttcac tatatttttc aggagaaaaa acgcgataaa ttgacaaagt taaatcctct
    14581 tctaaatcta ctcgataatg atacaatcga gtcaatcgtt ctatatcttt tttcgttagc
    14641 aatacttgat taataacttt tcctgtaaaa acacgttctt tgcgcaaaaa gcggtaatca
    14701 gttacttgtt caatacgatt caaccaatca taaaacgttc tggaagaaag agaaggatta
    14761 atcattcgta ttgctgtagc tactctataa taacaactct ctgccattta acttcacctc
    14821 cttagttgat tacaaaatat gcgcatcaga aaaacttgtt ccttttggat cttttgctaa
    14881 atacatttgg cgctctagtt gttcttgtaa caactgatta tcatctccca tcgtttgatc
    14941 ttgactatcc tcttttactg taaaatcata gacaatcatt tcagtaaatt tctcaaattc
    15001 tttgataaag tgataatctt tgtaaacaaa tggcgcaaaa aactctacag gtacatcaaa
    15061 tcctggaaaa gcatatccac gaccaggttg atctttgttg taaaatggct tatttttaaa
    15121 atcttctcca aaagtcatta aataaccaat gggagacaat cgaccaacag aaatacggaa
    15181 tgttaaatta tcccgaacac cacttggaaa atattctgca tctggtcgtt gcattgctaa
    15241 aataatgtac atccctgctt gacgaccctt taatactaat ggctgaatca atttatcgac
    15301 tctatttctc gcttccatat ccaacgaacc ataaaaagct gcccattcat caaaaaagat
    15361 aaaatgcggt ggtaacccat aataagcata atttttccct ggttcatatt taggatcttt
    15421 catggttttc atataggcat accgttgatt catttctttt aatgcatttc gtaaacaaat
    15481 aatcatttct tcatttattt tgtaaaacac atgtcctttg aatacatcga cgtcacctaa
    15541 tgctgctaaa tctgccgctt ttccatcgca aatatctact gtcccaacac ttaaacaagc
    15601 ttgaattaaa gaatacaata aataggtttt cccaccaccg attgcaccag caatcaacat
    15661 atttggtgat tgatcatata cccaatccaa agttttcatt aaatgaatac gtccattttc
    15721 tgctatacat tgatcaatag gaatccgacc agaatctgaa aggaatacat aattgattcg
    15781 ggctttttct cgttcttcac gaattaaatc acgcaaaaat acttcagata acaaatgacc
    15841 catttttaaa aatcgttcgt gaaaacgccc accatctaaa ggaaaagaaa ccactaattc
    15901 ttgtttcttt tgataaaaat aaacttttgg taatctcatt ttttcccttg tttgtttctc
    15961 actaacttgt ttctttgtat aaacaaaccg attgtcaatt aacattcgaa caagtaactg
    16021 acgccaataa acagaggcaa aatatccttc ttttagtcgc gaccaatgca tcaaccctaa
    16081 aattaaccca ctaaccagca acaaatacaa cacaatactt attccaggaa taagatacat
    16141 ttgccaattc gaaaacgtac ttaaacgtgg ataaaatact ttccaactaa accaacctaa
    16201 cagaatcaaa aatggaaaca aaaagaaacc ataatagcaa aaccataaat aacgattgaa
    16261 atagcgaatt cgccgaccac gatacttgaa aaaatgagct ttcaattgtc acattcctcc
    16321 tttctgtttt tacttttcct gtgctaaaaa cttttgacaa aaagcaacta atacaggaat
    16381 atcacattta gtaaaaaaac ataatcgaaa aagatttaat cgatttttat aagggtgtgc
    16441 acacatatta catattcgta ctatacgaat ccaaatcact tcctgatgat cttgtttcca
    16501 ctggtacggt aatgaaaaac gaacaagatc acgtaaaaac atacactgcc caataaccgc
    16561 acaaatatat gcatttgttt gtacaaaaat cgttggattg tctccaaaat actcaatcaa
    16621 ttgttggata ttttgataac aagcacaatc tctcaaagta tttgattggt tttatctgaa
    16681 ttcaaaatct ccccttcctt ctatcttaga taaaaagaat aggaagactc cttcctattc
    16741 tttcaaaaaa accttatttt ttctcttttt ccttagaatc aacacttggt tgtttttgat
    16801 ctaaaccttt ttcttgtgat tgtttcatat taggattatt tgttggagct tctactttct
    16861 ctaatccttt cgcataaatc actaaatctg aatattcacg attgccatct tgtgctggaa
    16921 tatataccac atgatattct ggatcaatta atttcacagg atcacgtcgt ttaaaaccag
    16981 tcaaatctac atctgctgga aatttaactc gtcgtgcaat ctttggttca tctggatttc
    17041 caaaaactaa ttgtgctcgc tgtgatcgaa tttctcccgt ataatcgcct gtttcattat
    17101 caaatacacg atcagactct tctaatgcgc caaagcgtac ggctcctaaa caagtttcat
    17161 ctaatgcttc tgtaatgtca taattatttg gaaattttaa tcccatctta caaaacctcc
    17221 ccaattttta caccagttaa aaagacacca acatcttgat agccagttgt tcgacgctct
    17281 gtttgtccca tatcatttgt ttcacgacta ttcactcgtt ctttacgaaa aacaaaataa
    17341 gcttcttcaa atgaaatacg cacctctttt tctggtaatc gtaatctttt atcctcttca
    17401 gctgtgagat aaacaaaaaa agcaggctgt tcttctcgcg aacactctgc ttgtaattga
    17461 acatatcgcc cagacacacc attaatttcc tttggtggat taggttcccg tgaacccaat
    17521 aaaaatactt gacctaaaaa ctctttcttc atacgcaatg ctttttctgc cataaagttt
    17581 ttcctcctct taaaaaccaa aataaattct aaatatttct acattacaat caaacaagcg
    17641 gttaaaaatc ctaaccgctt gttcctcatg actaatcttt ggctttacgt actttataaa
    17701 gtgttatacc taaagcaatg actaatacta ctaatccaag aaccataagt atactacttg
    17761 tttgttcacc tgtttttggc aacattggta caacagtagc tggtttaacc cgttgttctg
    17821 gtttcactgg ttgcggtggt tttggcgctt ccacaatttg aaccgtttga tctttgtttg
    17881 ttagatctac attgtgtttc gccacttctt caaacgtttc tttctcatct tccgttttcg
    17941 cttggagcca ttccgcaaaa ctcaagcctt ttcctgcaag attgcgaccg tccactaaga
    18001 attcaaataa gaattccatc atttctttat cggccgcaaa gactttttcc gcatgagtgc
    18061 cttcaaatgg tgtgccgtct ggtgttaagt agccttgaac aattaagcga tagatttgac
    18121 caatcgcaag accgtctact ttgacccatt caagcacagt ttgatttggc aaggccaaga
    18181 tctcttttcc gccattttta ttcgtcgctt tcgtcgtaat ggttggatta ctgattttga
    18241 tggtttggtc attgtcggta atatctgcgt gagaagccaa taatacgcct tctctaaaca
    18301 gatcttcaaa gaccacgact tctttgcctc gcaacgcacg tgcgtctaga ataaagtcta
    18361 gattgactgt accatccgtt gtttctgcca caaacgttaa ggtacttgtg acttcttttc
    18421 catctactag cactggtttt tccgttactt tgtccattag aacaccgttg actgtgtatg
    18481 tttttccaac gaccaaatcg tggtatttca cagcgtctgt aatggtgact ttcgttaatg
    18541 gattaacctc tttcaagcca gtgggtttat gtgtagccgt tgtacgtaca cttgggttag
    18601 ttactcgtac cgtttgccct ttgtcgttga tgtctttatg gtttgctagt tcttcatcat
    18661 ttctaaaaag actttcaaac acgacgattt cacgaccatt tatacctgtc aaatcaaagg
    18721 taaatggtac gatcaccgtt tcattttctg ctttggcaat aaagccaaca ctcgtttcta
    18781 ccgtatttcc atgcacaagc aagggttcac cggttgcttt atccattaag atccctttga
    18841 ccaagtatgc ttttcctgga agaatctcgg taatttcaac ggtatcgttt aaggtaaaca
    18901 atcctgttgg gtctgcgact gtcaatccgt cttcaaagac agccgtggta tgaacggttg
    18961 gattattcac tttcacactt tgggcttcat cgttgatatc cgcatgtgct gcaacttcaa
    19021 tgccatcacg gaaaagtttt tcaaaaacaa cgatcgtttg acctttcaga acactagcat
    19081 taaccacaaa ttcaaggtct tttgtgccat tgactttgtc tgcaataaag gccaaggtac
    19141 ttgtgatttc tttgccatct tgtaaaattg gcgcattcgt tgccttgttc atcaatactc
    19201 ctgaaatggt gtattctttt ccgacaaaca agttggtata agaaacactg tctttaatga
    19261 cgatcgtttc taatggatca accacttttt caccatcggc tttatttgac gcttgtgttt
    19321 ctagttttgg tggaatcaag taattacgta cagaaaccac taacgtttcc ccatcatctg
    19381 gcgcattttg tttgaatgca accgtgaatg cttgtttatc tgtatttggt tggaaacttt
    19441 ctaatggggc attttccaag aagtaatagt catcggcaac tagacctttc actaaaattt
    19501 ccccgttttg atcagtggta tattcaccaa ttgcttgatt tttaccgtta tacaaggtaa
    19561 agttcacacc tgcgagcggc gttttcttct tgttccattc ttcatacttg aataatttta
    19621 cacttttccg tgcttgacgg ttcgcaaaat ggatcacttt gatcggttct gtatcattgg
    19681 taatcgagaa cactaatggg ttttgattca aaacgtaatt tacgggggct ttgatttctt
    19741 ccaaacgata gcgatctttt ccataagtaa gtgtaccgtt agttaggaaa aagccttttt
    19801 cattggtttt aaagatatcc gttgtttctg tatcatttgg aatctccatt gtgacataac
    19861 gattggctaa agtatcaaaa atcttgaatt ctgtattagc aagtggaatc acttttcctg
    19921 tttcttgatc cactttcacc actttaagac gttgttcaat tactttatct tctaaaacat
    19981 aatggaaagt ttgcccattt tcagaaattg tgacttcaag tggttcaaat agtttatacc
    20041 cttcttttcc tttggtttca gaaagaatgt acgtatcaaa gacaacggta tttttaccaa
    20101 aagtagcata accatcattg tctgtcacaa cggttttcac cactttgcca gttgtttttg
    20161 acgtcaaagt gatttcaatg ccagctaatt ttggtttgtt atctggattc gttgaatcga
    20221 ctaaatcttt attagcaact ttcactaaat caatggttcc tttgatcact tgatcttttg
    20281 cggtcgcatt gacagttttt ccaggttcaa caacaatttc aattgcttca tcagaaagca
    20341 cataaccttt tggcgctttg atttctttta aaacatattt gccaggatca agatctttaa
    20401 tttctgcctg tactttgccg ttaatttttt tcaaggtaat ttctttaatc tttttcccat
    20461 ctgcttgata caaaccatag accgcatttt ctaaagaagc tgcaccttgt gctttcacac
    20521 cagtttccgc atcttcttta aacacacgaa tgtttccttg ttttagaatt tgtaaattaa
    20581 taacagacgc attaggttct gttggatgta acacaccaat tgtttgagct cctggacgac
    20641 gataggctaa tggcgcacct tgaactttaa ttttattcaa ttgaattttt gctttatccg
    20701 gtgcctcttt tgtgactgta atccatactt cattgccgtg aatagataca ttgacacctt
    20761 ttggtttttg aatgtctttc acatatttca aaaaatcatt tttattcttt actttcaacg
    20821 tttcgccaat ttttaccgta tgtgtttgat tattaaaagt tgctttttca ccaaagttat
    20881 taatcaaacg ttgaatttct gcttttcgac gatcataatc aacacggata tttttgattg
    20941 ggatttttgt gcccaaagct tcccaaatca taaattgtgc cgcaatgtat tgttcatcgg
    21001 atttgtcttt atttgtgaca tatcctaaat aagagatcaa gcttgttttt ttccgtacat
    21061 ttgaattttt caaatagttt ttcaaagcag tagctgtata aacttgacct tctgcggctg
    21121 agacaccagg ctcaatacaa aacgccactt gtccattaat ctcaaaacga accatataat
    21181 ttaaagtgaa gtttagattg ttcggactag aagtaacacc aatattttcg ccccaatcaa
    21241 ttttccgacc acctaaaaca gctgcattgg ttgcacggcg aacacgaacc gaacgattgg
    21301 ttggtactgt tactaacagt gtaggtgtac ctgtttttgg aatgttctgc tcttttttct
    21361 tttgattttc aatactagcc gtagattctt tgtttggttt cgttggttga ttttgactag
    21421 ctgcggttga agagattgtt ttttcaacgt tttcctcatt ttttccttct ttgtcagcag
    21481 tagccgctgg ttgttccact gtttcttcta ttcgtttctt ttcttccgct tgtttctcag
    21541 aagactctgg ctgcacagaa ggcgttgctt cggcagcttt ttctccttct agagtggacg
    21601 tttctgaagc aggggttgta attgaggcag tttcttcaac tggttgtgaa gtaccaggac
    21661 tattccctgg gtgttgtgtt tccccaatcg tctgatcgag agtcgttttg gaattgaccg
    21721 ttggcgcttc tgtcacaggg gcctgttcaa cagtggcttc accagctggg accccggctt
    21781 ctgctggttg gacttctgtc acttcagaag cgacttgggc aatggcttcc atcgtaggtt
    21841 gtatagccgt cgtttcttct gcatgtgctt gagcagttcc caccaagaaa cagaattgag
    21901 aaaaagccac caatgcgagt gctttcaacc aattcatttt cgtttttgca tacacaagaa
    21961 ttcctccttt tcctgagaat tttatataat aaaacaaaac gccgttacca aaagtgataa
    22021 cgacgtgaac tgtctcttct atttttatct ggcgctctta tctgaacaca tttcatcaat
    22081 gattttaccc tcccactata attacagaac aaaaaaagaa cctgcttcgt ggaaatcaac
    22141 tgtccatttc ccaattggaa taccagcttt tttggcaact tcaccatcac caaatctcat
    22201 gccataccaa ttactacctt catccttaat ttctttccaa ccatatgaca tagcttcatc
    22261 taatgaatca aaaatcatga cactattgcc aattgttaca ggatctggct taagatcttc
    22321 tggaagagaa atatcaatca taccataatg atccgattcc tcagtattat tgtcattact
    22381 agctactggt ttggttgttc tactggctta ttttgataca acaagtactc ttattcttct
    22441 tttacttctc cgttttcaat ttcccattca aaccactccg caaagtcttg cttaaaacca
    22501 tctactcgat acctattttg taaataggca tcttctgacg tcgtaggatc taaataggta
    22561 ggtttattct ctggacgagc atagacacct acccatgttt cgtaatctcc ttcaaaataa
    22621 ctatctacca aatagccttt ttttctaaga atctcccgaa cttgattttt aattattgat
    22681 acttgttcaa aaccatcact cattctatca cctcaaataa ctctagattc tcataaatat
    22741 tcccgacaat ttcaatccta tcaatgatct ctaacaaatc gctacttatg cttccccatt
    22801 cgtataagaa tttcccttcc gcaaaataaa ttttccataa acttcttcat gatcgtccca
    22861 accaatatca ctctcgaaaa attccacgtt gttcttatct tttagctcag taaatgccat
    22921 aagttcaaca tgttttccaa cctcgaaaca atcacaccac ccatcaaccg ttacacgtgt
    22981 gccttcaata cctaagaaac tttgtcctcg tgtattaaac actaaaactt tccgcatttt
    23041 ttgctcatta attaatcacg cgcgaaatct aggaatcaca acttattcgc ttcttcttta
    23101 taaataaaaa aactccaata atcggaaatt actatctttt cctcattcct tttttaaatc
    23161 ttcatacttc ttcttatctt tgtcctaacc taataacaca aattttgaag aaaaactcag
    23221 taaaacctga ttcttttttt atcttcacaa caaccaactt tttcttcaga tccttttact
    23281 agaacttgta tacaaaaatt gttgaatgct gatataattt gcggctactt gaaaaaacga
    23341 tagatgtaaa aagataaata gaatcatttt ttattctata tcagtctatg attcattgct
    23401 tatcaatcac ccaacccatt cgatgccgtt ttttttagag aaatttaaaa aagaccgtac
    23461 gaatctcgca ttatccgatt acttttccag aaaagatccc caattctgta taaataaccc
    23521 tatcttcttg ttggtttcat tttctcatat aagaattcag tcaatctacc cactatgacc
    23581 attactacgt ccaacacaac tgacatatta accacgtaag tattccctca cagatctccg
    23641 aatctccatg gtatggattc ccatcctgac gctgcagagg acaacatatt tcatgtggct
    23701 ctgtaccctt ttgatttgga aacactacaa atcaatactc atctataact tatcaagcga
    23761 actttcgcta ggcttctagt gattactaaa ttgttggata acatacatag cccattttat
    23821 ctagctgtta ttcataacag ttagttcttg tattacttct tccgcttgtg ttgccattga
    23881 gcttgtcaaa aagttactta gaaaaaagct gccagtaaag caactttcaa caaacccttt
    23941 ttgtttctta tcatatcaag aaacctcctt ttataaattt tttctataat aaaaaacgtc
    24001 gataacttaa taagtatcga cgtaaactat ttttatatcc cgtaaatcaa aaattctaaa
    24061 gaaagatttc ctatataagc atattggata attcgattag tattgggtat atctctacca
    24121 ttctcatacc tatctatgga ccattttgaa attgaaggag aaaaaagctt tccaaactcc
    24181 tccctttcta gataataatc tttcctaatt ttcctcatcc ttaatcccat actaaaagaa
    24241 tttatctttt taaaatcgct aactctttca tcttttggaa gtatattctt ataatcttct
    24301 ccgtacataa ggtaaggaac agtgacctta ccaagatagg caatattcat tagtttttca
    24361 atatctggaa tattaatacc tttttcccat ctacttataa catttttgcc tgtatagatg
    24421 gtaaagtttt taccaaattt ttcaagcgtc tcttcccggt tctttcggac ttctcgtatt
    24481 ctttttccca taacaatttt atcgtctttt gataaactaa atatctcact atcacgatta
    24541 atctccttca aaatttcttt ttcattactt aatagaaaaa tatcgatttt ttttgcattt
    24601 cttttgacgg atttctcttt ctatttaaca cataactgag taatacttca ctaattccta
    24661 tattatcagc taactctttg ttaagtaact tattcatatc tctgtatgca attagtttac
    24721 cgatgagaat ctcaatctta tcttcctcat cttttattag agtttctata ttcagaatct
    24781 tgcttagagt attatctacg tctttaggga tttttcttga ccctttttca tatttaacta
    24841 aagtactttg tgaaatacct gctaaatcag ctaacttttt ttgagatagt tttttctctt
    24901 ttcttagaga tttaagaacc cttccacttg aattatcaac catgaaaaaa gcacctccta
    24961 tgagatgctt tcatcatata ctgacagcta aaaatcttca agaaaaatat tccaattttg
    25021 ttaattttag tatcgccgtg ataaccaaat gacagtttaa aatcttttat acagataatc
    25081 ctcagtgttg catctactca aaaaggttta tttaataggt ttttcgattg attataagtt
    25141 atttcacact atcttgctca tccattttca gaacggccat gaaggcttct tgcggaactt
    25201 cgacggagcc gatttgtttc atccgcttct tcccttcttt ttgtttctct aagagtttac
    25261 gtttacgaga aacatctcca ccataacatt tagccaagac gtttttacgc aaggctttga
    25321 tgtctgaacg ggcaacgatt ttttgtccaa tagctgcttg gattggcacc tcaaattgtt
    25381 ggcgtgggat tagttttttc agtttttcaa cgattgcttt tccacgttca taggcaaagt
    25441 ctcggtgaac gataaagctt aatgcatcga ctttttctcc attcagcaag atgtccatct
    25501 tcactaactt gcttttttga taaccagaca tttcataatc caatgaggca tagcctttcg
    25561 tgcttgattt caatttgtcg aaaaagtcaa agacaatttc agaaagcgga atattataga
    25621 cgacatttac tcggtaatca tctaaataat ccatcgtaat gaattctccc cgttttcgtt
    25681 gagaaagttc cattactgca ccgacaaaat catttgggac catgatctgt gctttgacga
    25741 atggttcttc tacatcctga atcgttacag gttcaggaaa atctgccggg ttatctactg
    25801 tagcagtcgt accatccgtt ttattaacat ggtaaataac ggatggtgca gtcgtgatca
    25861 attctagatt gaattcccgt tccaagcgtt cttggacgac atccatatga agaagtccga
    25921 ggaatccaca gcggaaccca aaacctaatg cttgagaggt ttctggttcg aattgtaaag
    25981 cagcatcatt caattgcagt ttttctaaag cttcccgcaa atcgttgtaa cgagaagtat
    26041 caattggata aagaccacaa taaaccatcg gattcatttt tcggtaacct ggtaaagctt
    26101 cagcggcagg attgtctgct aacgttactg tatcccctac tcgtgtatct tggacagttt
    26161 tgatacttgc tgtgatataa ccgacatcac cgaccatcaa aaagtctcta gcaactgctt
    26221 ttggcgagaa gaccccgacc tctgtaacat caaatgtttt gccattactc atcagtttga
    26281 ttttgtctcc gggtttgacc ataccgtccg tgatccggac atttagaaca acaccgcggt
    26341 aactatcata aatagagtca aagatcaaag cttttagcgg tgcatctaag tctccacttg
    26401 gtgcaggaac atattccacg atctgctcga gaatatcttc aatcccaatc cctgatttgg
    26461 cactggctaa tacagcttca ctagcatcga ttccgattac gtcttcgatt tcttgacgga
    26521 cacgttcggg atctgctgcg ggtaggtcga ttttattgat gactggcagg atctccaaat
    26581 cattgtctaa cgctaaataa acattggcca atgtttgtgc ttcgattcct tgtgctgcat
    26641 cgaccactaa aacggcaccc tcacaggcag caaggcttcg agagacttca taggtgaagt
    26701 cgacgtgccc tggggtgtca atcaagtgaa agatatatgt ttctccattt ttcgctgtgt
    26761 aattgagttc tacagcattt aatttaatcg tgattccacg ttcacgttcc aaatccatcg
    26821 agtctaacag ctgatcttgc atttcgcgag acgtcacagt gtgcgtcatt tctaaaatac
    26881 gatcggccaa tgtagatttc ccatggtcga tatgagcgat gatcgaaaaa ttacgaatct
    26941 gctcttggcg ttctttcatt ttctttatat ccatagcgtc tcctcttttc atataatcag
    27001 cactttccat tataccaacg aaaagcagta atttaaagag tttttaatta gtcagctata
    27061 gaagtttttt ttgtagaatt ttgctatact gttatcaagc taataaatgg agatgaaaaa
    27121 atggatcaaa aagattttag agaaaatctt gaattaaaac cggtcactca agaacatatc
    27181 gatcaattca atgaattgct ttcctatgtg ttccaagtga ctgaagcaga catcgaggaa
    27241 agcgggtttg agaataaacg agcttttatc cgttccaaaa aaccgatttt ggaagtatcg
    27301 aaagtgttcg gatggttcca cgaaaataaa ctgatctctc aaatcgctat ctatccttgt
    27361 gaagtcaata ttcacggtgc acgttataaa atgggtggtg tgactggagt tggaacttat
    27421 ccagaatatg ctaaccatgg attgatgcaa gatctaatta tcgttgcatt ggataatatg
    27481 cgcaagaata agcaatggat ctcttatttg tatccttaca gtatccccta ctatcggcgt
    27541 aaaggatggg aaatcatgtc tgataaactt tcctttaaaa tcagagatac acagctacct
    27601 aaacaaatcc cagtaagtgg tatggttgag cgattaccag ttgatcatcc agacgtattt
    27661 tccgtttatg acaaatttgc ccaacaaaac catggtgctt tattccgcag tgattttcat
    27721 tgggaagagt attggcgttt cgaaaatgaa gatgagcgca cagccgctgt ctactacgat
    27781 agcaaccatg aaccaagagg tgttctgttc tactgggtag ctgaagaaat atttcacgta
    27841 aaagaaatgt tctatcttga tcaagaagct cgaaatggct tatggaactt tatttccgca
    27901 catttttcca tgatctactg ggtacatgga gatatttata aaaatgagcc tttagcattt
    27961 ttgattgaag atagtcagat caaagagcag atcgaaccat attttatggc tcggatcgtt
    28021 gatgtaaaag aattccttca acggtttccg tttgttggaa cagctgatgc ttttcatttc
    28081 atcattgaag atcctgtcgc tccttggaat aatggtgtct tcgctttaac atgggatgaa
    28141 cagggacaag tccgtgtctt aaatgaacct ataggaaaat ccgttcgact ggatatccaa
    28201 acattgactt gcttgatgat gaattatcgc cgtgcttctt atttggcaag aatcgaacgg
    28261 ttagaaacag atgaagaaac actgaagtca ctagagcgta tcattccaaa catggaagct
    28321 tactttagcg attatttttg ataaaaagga tgatgtttag atgaaaattt attttgccgc
    28381 accaatgttt gctaaaagtg atttgttata taaccaacag ttagttaaag aaatcagaga
    28441 gctctcttct gagctttcga tctatcttcc tcaagaaaat gaagcaatca atgacaaaac
    28501 tgcttatgca gacagtcgaa tgattgctct tgctgacaca gaaaaagtgt tggctagtga
    28561 tttaatgatt gctttactag atgggctgac gatcgatgcc ggggtagcat cggaaatcgg
    28621 tgtggcttat gcgaaaggca tcccagtgat cggcttatat actgatactc gacagcaagg
    28681 cggaacccac ccgaaaaaaa tcgctgcact acaagaaaca gctgaaaatc aatttcacta
    28741 cctgaactta tatacaatag gtttgatcaa attgaacgga aaagtggttt cttcagaaat
    28801 tgagttgctt tcagaagtaa aaagattttt agatggaggg actttcagtg attaaagaaa
    28861 tcaaaaaggt gcaaatagct ttacttgctt ttggtgtatt cgttgtctgc tataattttt
    28921 atgaatttat cacacaaaaa tattcgactt ctcaaggaat cacatttatc gtggaatctt
    28981 tattagggat tgcattgatt ttcatgccac aagtcatttt gaaagttttc aaacttaaaa
    29041 taccagcagc aatcgttttg ttttactggt ttttcttgtt catctctgtc tttttaggca
    29101 cagggatgca tttgatcagt atcatctctt tttgggacaa aatcttacat gcggtgagtc
    29161 caatggtcct gacagctctt ggctatggat tgattggtta cttgatgaaa gatgctgaga
    29221 tatcaaaaac cagtccttgg ttgttcctct tattcggctt tgcgtttgct ggactttgcg
    29281 gcgtgttttg ggagttctgg gaattcttat gcgaccagtt cttaggcatg aatcttcaac
    29341 gttttgctgc ttctgatggc acactgtttg tcggacgagc agcattgatg gatacgatgg
    29401 gcgacttgct gacaaatacg ataggtgctg ccttaatggg attgtttgca tggtctcaaa
    29461 gtaaaaaaga tgagcgttac tttgaaagtt ataaattaga aaaagtgaaa aatacctaat
    29521 aaaagaggtt gtgacagaag cgggcagctt caagaaattt cggcttattt ttcgaagaaa
    29581 ctacttctgg aacactgttt atttgaaaaa aggaggtctg acactagttg tgtcagaccc
    29641 cttttttctt ttatagtctc aatggaagat tagacgattt cttctagctc ttctgttttg
    29701 aaatgaagtt gtccatcaag taaagtaatc gttacttttg atttaggcat aacatgtcct
    29761 gcaacgattt ctttagctag tggtgtttct acttccttag tgataaatcg tttcaatggt
    29821 cttgcaccat atgctggttc ataagcattt tcagcaatcc acgttttggc ttcatcactg
    29881 attgtcagca ggatttcttg atgttccaga cgttgtgcca attgcgccac cattttgtca
    29941 acgattcctt tcacgttgtc taggctcaat ggtgtaaata aaatcgtgtc gtcgattcgg
    30001 ttcaagaatt ctggtttgaa gtttccacgt aatagcgtgt tgacttgttc agccactgct
    30061 tctggaatcg ttccatcagc agtcacgcct tccagcagta actgtgaacc gatattgctg
    30121 gtcatgatca gaaccgtatt tttgaaatct actactcgcc cttttgaatc agtcaagcgt
    30181 ccgtcatcta ggacttgtaa caagatattg aagacatcgg gatgcgcttt ttcgatttca
    30241 tccaacaaga cgattgtata aggatttcgt ctgacagctt ccgttaattg accaccttct
    30301 tcgtaaccta catagcctgg aggagctccg actagacgag acacagcatg tttttccata
    30361 tattcactca tatcgatccg taccatatga tcttctgagt caaacaagtt ctcagctaac
    30421 gctttcgcta attcggtttt tccgacacca gttggtccta ggaaaaggaa agaacctaga
    30481 ggacgatttg ggtcttgcaa tccagcccgt gaacgaatta ccgcgtcact gaccgcatca
    30541 actgcttcat cttgaccaat cactcgttta tgaagtgttt cattcaattt aattagtttt
    30601 tctcgttcac cttctacaag tttcgttacg gggatacccg ttaatctgcc aacgacttga
    30661 gcaatctcat tttcggtaac ggactcttga accatcttaa tctcgctgtc ttttgcttta
    30721 gcttctaatt ccttcaattc tttttctaat tgaggtatcg taccatgacg caatacagca
    30781 gcgcgttcta aatcatagtt attttctgca tcttctaatt cgtgttttgc tttatcgatt
    30841 tcagcacgtt ttgctgaaac agaattgact tcttcttttt ctgtttccca ctgcatcttc
    30901 atcgcatttg cttcttcacg aagttcagct aattcttctt gcaaattttt caagcgtttt
    30961 ttacttgcat catctgattc ttttttcagt gcagcttctt cgatttccaa ctgcatcaaa
    31021 cgtctagtaa cttgatctaa ttccgttggc atagagttca tttctacccg aatcgttgca
    31081 ctggcttcat cgatcaaatc gattgctttg tccggtaaga accgatcagt aatataacgg
    31141 tcagataaag tagctgctgc tactaatgcg ttgtcatgga tgttaacacc atggtggatc
    31201 tcaaaacgtt cttttaatcc acgcagaata ctgatcgtat cttccactgt tggttctttg
    31261 actaatacct tttgaaagcg acgttccaac gctttgtctt tttccatata ttgacggtac
    31321 tcatctaaag ttgtggcacc gatcaaatgt aattcaccgc gagcaagcat tggtttcaac
    31381 agatttccgg catccatact tccttctgtt tttcctgccc ctacgatatt gtggatttca
    31441 tcgatgaaca agaggatacg tccatcactt tttttgactt ctttcaagac tgcttttagt
    31501 ctttcttcaa attcaccacg gaattttgct ccagcgatca atgcacccat atctaatgaa
    31561 aaaatcgttt tatcttttaa attttcaggc acatctttgc gtacgatacg ttgagcaagc
    31621 ccttcaacga ttgccgtttt ccctacaccg ggttctccta tcagaacagg attatttttt
    31681 gtttttcgtg aaagaatacg gatgacatca cggatttctt catcacgacc gatgattgga
    31741 tccatttttc cgcttttgac ttgctgtact aaatcaacgc cgtatttttc taatgctttg
    31801 tattgttctt cttggttttg tgaggtcact ctttctcctc ctctcatctc ttcgatgttt
    31861 ttacgcaatt ctttttcaga taatccgttt ttattcaaat aaacagttaa cggataattt
    31921 tttaatttca tcaacgctaa aataacgact tccgttgaaa gaaactcgtc gccgaagctt
    31981 tcacgtattt ggtctgcttc attcaataat cgaaagaaat tctggctgag gttttgtcca
    32041 tattggacgt ttccaccaga aacaactgga tactcatcca gtagacgatc aatttcatgt
    32101 tcaaaagatt cgacatccaa tccggcatcc gtatagaaat ttcgaccaaa atgattagat
    32161 tgtaaaaaga ttttccatac atgggcaatg tcgatatctt gatgttttcg agtgaccgca
    32221 atctgttgag cttcagcaat cgcttcttga agcgtcgtag tcattttctc aatattcata
    32281 caggtatacc ctcctaatta aaggtaagat cacttatttc tttgatcttt acaaaaacta
    32341 ttatat
//
LOCUS       DAZULG010000019        32265 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000019 DAZULG010000000
VERSION     DAZULG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.019"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1285)
                     /locus_tag="ACG9CY_000823"
     CDS             complement(26..1285)
                     /locus_tag="ACG9CY_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS4937354.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(1456..1902)
                     /gene="dtd"
                     /locus_tag="ACG9CY_000824"
     CDS             complement(1456..1902)
                     /gene="dtd"
                     /locus_tag="ACG9CY_000824"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS4937355.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(1913..4126)
                     /locus_tag="ACG9CY_000825"
     CDS             complement(1913..4126)
                     /locus_tag="ACG9CY_000825"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS4937356.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(4359..5123)
                     /locus_tag="ACG9CY_000826"
     CDS             complement(4359..5123)
                     /locus_tag="ACG9CY_000826"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS4937357.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(5124..6071)
                     /gene="prmA"
                     /locus_tag="ACG9CY_000827"
     CDS             complement(5124..6071)
                     /gene="prmA"
                     /locus_tag="ACG9CY_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS4937358.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(6084..6572)
                     /locus_tag="ACG9CY_000828"
     CDS             complement(6084..6572)
                     /locus_tag="ACG9CY_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS4937359.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(6538..7209)
                     /locus_tag="ACG9CY_000829"
     CDS             complement(6538..7209)
                     /locus_tag="ACG9CY_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS4937360.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            7347..8633
                     /locus_tag="ACG9CY_000830"
     CDS             7347..8633
                     /locus_tag="ACG9CY_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS4937361.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            8705..8899
                     /gene="ssrS"
                     /locus_tag="ACG9CY_000831"
     ncRNA           8705..8899
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG9CY_000831"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            8977..9261
                     /locus_tag="ACG9CY_000832"
     CDS             8977..9261
                     /locus_tag="ACG9CY_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937362.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            9334..9813
                     /locus_tag="ACG9CY_000833"
     CDS             9334..9813
                     /locus_tag="ACG9CY_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4937363.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            10036..10179
                     /locus_tag="ACG9CY_000834"
     CDS             10036..10179
                     /locus_tag="ACG9CY_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS4937364.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            10524..10652
                     /locus_tag="ACG9CY_000835"
     CDS             10524..10652
                     /locus_tag="ACG9CY_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937365.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            10757..12340
                     /gene="abc-f"
                     /locus_tag="ACG9CY_000836"
     CDS             10757..12340
                     /gene="abc-f"
                     /locus_tag="ACG9CY_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS4937366.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(12777..13961)
                     /locus_tag="ACG9CY_000837"
     CDS             complement(12777..13961)
                     /locus_tag="ACG9CY_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS4937367.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13986..14993)
                     /locus_tag="ACG9CY_000838"
     CDS             complement(13986..14993)
                     /locus_tag="ACG9CY_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4937368.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(15165..15524)
                     /gene="comGG"
                     /locus_tag="ACG9CY_000839"
     CDS             complement(15165..15524)
                     /gene="comGG"
                     /locus_tag="ACG9CY_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS4937369.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(15496..15921)
                     /gene="comGF"
                     /locus_tag="ACG9CY_000840"
     CDS             complement(15496..15921)
                     /gene="comGF"
                     /locus_tag="ACG9CY_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS4937370.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(15884..16231)
                     /locus_tag="ACG9CY_000841"
     CDS             complement(15884..16231)
                     /locus_tag="ACG9CY_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937371.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(16203..16673)
                     /gene="comGD"
                     /locus_tag="ACG9CY_000842"
     CDS             complement(16203..16673)
                     /gene="comGD"
                     /locus_tag="ACG9CY_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS4937372.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(16648..16926)
                     /gene="comGC"
                     /locus_tag="ACG9CY_000843"
     CDS             complement(16648..16926)
                     /gene="comGC"
                     /locus_tag="ACG9CY_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS4937373.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(16923..17972)
                     /gene="comGB"
                     /locus_tag="ACG9CY_000844"
     CDS             complement(16923..17972)
                     /gene="comGB"
                     /locus_tag="ACG9CY_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS4937374.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(17929..18858)
                     /gene="comGA"
                     /locus_tag="ACG9CY_000845"
     CDS             complement(17929..18858)
                     /gene="comGA"
                     /locus_tag="ACG9CY_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS4937375.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            18990..20744
                     /gene="ade"
                     /locus_tag="ACG9CY_000846"
     CDS             18990..20744
                     /gene="ade"
                     /locus_tag="ACG9CY_000846"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS4937376.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(20842..21504)
                     /gene="trmB"
                     /locus_tag="ACG9CY_000847"
     CDS             complement(20842..21504)
                     /gene="trmB"
                     /locus_tag="ACG9CY_000847"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS4937377.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(21574..22356)
                     /locus_tag="ACG9CY_000848"
     CDS             complement(21574..22356)
                     /locus_tag="ACG9CY_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS4937378.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(22560..23771)
                     /locus_tag="ACG9CY_000849"
     CDS             complement(22560..23771)
                     /locus_tag="ACG9CY_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4937379.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(23764..24501)
                     /locus_tag="ACG9CY_000850"
     CDS             complement(23764..24501)
                     /locus_tag="ACG9CY_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937380.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            24639..25073
                     /locus_tag="ACG9CY_000851"
     CDS             24639..25073
                     /locus_tag="ACG9CY_000851"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS4937381.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            25066..25410
                     /locus_tag="ACG9CY_000852"
     CDS             25066..25410
                     /locus_tag="ACG9CY_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4937382.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            25590..26600
                     /locus_tag="ACG9CY_000853"
     CDS             25590..26600
                     /locus_tag="ACG9CY_000853"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4937383.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(26669..27613)
                     /locus_tag="ACG9CY_000854"
     CDS             complement(26669..27613)
                     /locus_tag="ACG9CY_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4937384.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(27597..30317)
                     /locus_tag="ACG9CY_000855"
     CDS             complement(27597..30317)
                     /locus_tag="ACG9CY_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4937385.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(30314..31534)
                     /locus_tag="ACG9CY_000856"
     CDS             complement(30314..31534)
                     /locus_tag="ACG9CY_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4937386.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(31815..>32265)
                     /locus_tag="ACG9CY_000857"
     CDS             complement(31815..>32265)
                     /locus_tag="ACG9CY_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFS4937387.1"
                     /translation="ELNYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLK
                     ANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLN
                     DLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         9560 a         6662 c         5632 g        10411 t
ORIGIN      
        1 tttttttcga taagtttatg ctaatctaat tcttgtaaag tagcgctcgg ctattcggat
       61 aagtcggaaa ctgattgaat ggaatcgtcc aatcttgttc aggtacagag aactcaaact
      121 tatttactag ctgatcggaa aacacacgta gactatgaag agtgatccat tctcctgcac
      181 agcgatgcat ttgtcgaaaa tttcccccgc cttgtgcaat catctcatat tcttctttat
      241 aggaaatatc ttttgctttc cctacatacc gtttgatcat aaaggaatct ggcgcttcaa
      301 ctgttcgttc atcatggtct gttccatata aatctaaaat aacccaactg ccttctggta
      361 tacgataacc atctacttct acttctttca gcgaaatcgc aggtagcatt gggaagaaag
      421 gataatagcg tctcatttct tgaataaacg gatcttgtaa cgtcgaaaag tcatttttta
      481 attgctgata caaatctggt ctactgaaaa gtgcatgtcc catcaatgcc gcccacacag
      541 ttagagcaac cgttggacgg atgatgttca ataattcaac agctgccact tccagaggta
      601 aaagctgacc atctaaatct gtggcattgg caaaagcata taatgccaca ttttcttttc
      661 cagccacagg attttgtcga gcttcttcaa tcaagccttg cgcccatttt tctgattttt
      721 tgcgattttc gacacctttt atgtgatcaa tcggagaagt aaacgttccg ctgatcatag
      781 agatttgata ttctgctaat ttttcgacct catctttaga aatcgctgat aaattgatac
      841 ctgcccactc gcaaatagat gtaaagaaaa ccatcttact taaatcaaat agttcaaatt
      901 gaccatgctg tgcttcaagt gcttgtgtca gattcttatc taagatacga tgataatctt
      961 ccattctttc cggtgtcatt agatccataa aaatattttt ccgatggtga tgagcagctc
     1021 catccaaagt ctgtactcca ccttgaccaa aaagtgtctt caatactaat tttggcatag
     1081 caccttttcg tttgaaattc cttggatcat aaaatacttt tgctgctgat gatccgtaga
     1141 tcgtgatagc ttctttattg aaaatctttg ctttgacgac gggagcgtcc gcttcatggc
     1201 gtaactcctc caacatgttg tatccttttt gatacaattt ttttaaatct gtgattttga
     1261 tatctactac tggtacttct ttcatcttca gcaacctcct ataaaaaata acttgtataa
     1321 tttaagctta actttttttc cgactgctga caaaagaagc gctttttaca gacaaaaaag
     1381 ccacttaagt tagagtttat cagtctaact taagcgacgt gtttcatagt ttttactagt
     1441 gaaaaccaga ttactttatt tgtttttaga atcaataatg atcgttacag gtccatcatt
     1501 gacgagagaa accgacatat ctgctccgaa ttgtccggta gcgactgtga tgccgcgact
     1561 tctgattcct tcattgaatg attcgtataa aggaatagct gtttctggtc gtgcggcttc
     1621 cacaaagctt ggtcgatttc cttttttcgt atccgcaaac agtgtgaatt gagagatact
     1681 taaaatttgt cctcccacat cttctacact aatattcatt tttccttgtt catcttcaaa
     1741 aatacgcatt tgtgcaattt tttttaccag atagtccaca tctttttgcg tatcggtttc
     1801 atgaatgcct aataaaacaa gtaggccgta gccaattttt ccaatttctt gctgatcgac
     1861 tgacacagca gctttactta cccgttgaac aactgctctc atatctgtcc tcctatccat
     1921 tcgtccggcg tacactgtag acatcaggta ctgctttgat tttatccacg atactcttta
     1981 ggtgggataa attctggata ccaaccgtca aatggatcgt cgccatttta tctttattcg
     2041 tccgtgcttc aacgctgttg agatttttcg ttagcgcatt gactgtttga agtacatcat
     2101 tcaataagcc tgaacgatta tagccgtaaa tttccaaatc tgcatcatac tcttttcgtg
     2161 tattcgatgt atcttcccat tcgacttcaa tcaaacgttc tgctacattt ggtttgtcag
     2221 gctgaacatt cggacaatct cgacgatgaa tggatatccc tctgccttta gtgatataac
     2281 caacgatatc atcaccagga atcggattac agcagcgact gatacgaatt agtaagtttt
     2341 ctaccccttg aatgacaacg ccaccttcat gacgtacttt cattttgtca ggttcttttt
     2401 tcggctgatt catgatttct tctgcttctt gcttttgctg ctcgattttt tgttctttac
     2461 gttctttttc agtcagacga ttggccatcg tcaaggggct aacttctcca taccctacag
     2521 ctgcatagag atcatcttct gtttgataat taaaacgatc gagtgcttct tgcagtttgt
     2581 tcttcgtcaa aatatcttta ggcgtaaatc ctagatctgt aatacatttg accacggatt
     2641 cgtggccttt gatcacattt tcttctcgat cttgggcttt gaagaaacgt ttgatcttat
     2701 ttcttgcttt gctagtagca actaatttca accagtcgcg acttgggcca aatgaatttg
     2761 gagaagtcat gatctcaatg atatctccgt ttttcaattt gtaatcaagc tgcaccattt
     2821 tgccatttac ttttgcacca gtggttttgt taccgatatc tgtatggata ctgtatgcaa
     2881 agtccaatgg tccggatcct tttggtaact cagtaacgtc tccttttggt gtgaagacgt
     2941 agactttatc actaaagata tctcctttta cgccttccat aaattcagaa gcatcatagc
     3001 tttcgtcttg aagttcgagt atctcatgga accagcttaa ttgtttcgtc ataccatctg
     3061 gttctacttt ttcatttttt ccttctttat aggcccagtg tgcagcaacc ccgaattcag
     3121 cgatttcatg catttcctgt gttcggattt gaatttctac cggatttcct gccggaccga
     3181 tcacggtagt atgcaaagat tggtacatat tagcttttgg catggcaata taatccttaa
     3241 atctgccggg cattggtttc cattttgtgt ggattgctcc taatacagca taacaatcct
     3301 tgattgaatc tacgatcact cgaatagcta gcaagtcata aatttcgttg aactgctttt
     3361 tctgatcttt cattttacga tagattgaat aaatgtgttt tggccgtcca tagatttctg
     3421 caaaaatccc cagctcttct gtcgctattc gaatatcttc aacagtacca cttacgtatt
     3481 tttcacgttc ttctctcttc gtctgcatca agtggacgat acggtaatac tgttttggat
     3541 ttagataacg aagtgctgta tcttctaatt cccatttgat ccggctgatc cctagacgat
     3601 gtgcaagagg cgcatagatt tctaaagttt cctgagcgat ccttctttgc ttatcttctc
     3661 gcaggtgctt caacgtacgc atgttatgta gtctatcagc taatttgacc atgataactc
     3721 gtaaatcttg agccattgct agcagcatct ttcggtggtt ttctgctagc tgctcttcgt
     3781 gggatttata ttttattttc cctaatttgg ttacgccgtc cactaacata gcaacgtcat
     3841 cgccaaattc ttctctcaga tcttctaaag tgatttctgt atcttcaaca acatcatgta
     3901 agaagcctgt agccactgta tggggatcca tttttaaatc tgctaatata cctgcgactt
     3961 ggataggatg aataatgtag ggttcgcctg attttctaaa ctgtccgtca tgtgcagcag
     4021 tcgcgtattc gcatgctttc tcaacaaatg ctacatgttc gggacccatg tattgactaa
     4081 cgagctttat gacaccgggt cccgttaaaa tttcttcttt tgtcacagga atcgatcccc
     4141 ctttcttttt tcggtaaaac gggaatcggg acaaatatct gtttgataat tgtcccaaaa
     4201 tccgtgcagt tatacgtttt tttacggaaa tgttcgcttt ttaaaagctt tttcaaacga
     4261 tttttcgtat ccattgtata aataattgtt tctattatac aaattgttga ctattttttc
     4321 aattctttct gtcgatactc ttttattaat gaagaacgtt agctgaaaga aggattcatt
     4381 aattcccatt gatagctagc agcacttaat aaataaagag gtgctgtttc tgtacgaagg
     4441 attcttggtc ctaatccaca aagtatccct ttgtgttcta caaaataatc gacttcatcg
     4501 cgggataatc caccttctgg tccaaagaca gctaagattc gactatctgg ttttgtttca
     4561 ctaagaattt tggctgcatt tgatttttcg ccttgctttg ctgattcctc ataagcaacc
     4621 aagaccacgt cataatcatc tagccgctca aataaatctt gtttcgtttc caataactct
     4681 actgatggca cgatctgccg atgggattgt tccgctgcct cttgagcaat cttttcaagc
     4741 cgttgttgtc gtttcatacg tttttttgca tcccatttaa cgacagaggc tgatgccggg
     4801 aaaccaagaa aagcttgcgc tcctaattca gtggatttct gaacgatcca atccagcttt
     4861 tcccctttag gatagccact cgctatggta atcgaacttg gcagttcctt ttgattttct
     4921 tcttttttaa tttctttcag ataaacagcc tcttcagtaa tctccgtgat ttctgcaaca
     4981 atggctattt gatcattgaa tgccaaaaaa acttcatcct cgggttccat cctcataacc
     5041 cgtacaatgt ggtgatagtt ttccccttct aaaacaaaat agtcttttga ttcatatgat
     5101 tcttttaaaa aataccgctg catctattcc acctctggtt ttttcaaaat aattgcatac
     5161 cagtcttttt gctggaaaat ttgatccacg ataaaccctt gttctaacat ggcatccaga
     5221 accatttgct tcttctcttc aatgattcct gaaacgatca gcgtgcctgt ttgcttcagt
     5281 aaacgccaag catctgggat catcaataag atgatatctg ctaaaatgtt cgcaacgatt
     5341 acatcacttt caatctcgat tcctgtcaaa agatcgttag ctgatacatg gacatctttg
     5401 gctatttcat tcatatccat attttctttc gccgaacgaa cggctacttc gtctaaatca
     5461 aaggcataga catcttttgc accaagatat tttgctgcaa tacttagcac gcccgaccca
     5521 gttcccacat ctagcaatgt ttcaccacca cgcaagactg tttctagtgc tgtcaatgtc
     5581 aaggaagtcg tgggatgagt gcctgtgcca aatgccatac caggatctag tgtgataatt
     5641 ttttctagag gatcttctgt ctggtagttt tcccagcttg gcgcaaccgt cagcattcgc
     5701 gtaatacgca ccggatgata gtatttcttc caggctgttg cccagttgct ttcctctact
     5761 tcactaactg ttactttatt ttttccaata gctagaccaa actcaggtaa ttgtgtgata
     5821 cgatctttga taaaaggcaa gatttccggt aagaaagtcg tttccggaaa ataagccatg
     5881 actagtgcgc cttctttcag cgatgtaaaa tcatttttat ctaaaatctc accatattga
     5941 tccgactgaa aatgttcaac atctaaagaa tcttcaatcg cgacaccact cgcccctgcc
     6001 tccatcaaaa tattagcaac tgcttcgact gcttcacttt cagtttctac ttttacttca
     6061 ttccatttca tatctgtcac tccttaataa cttgggtacg gtaaataagt atctgcttca
     6121 ttttgctgtt ttacttctac taattctgct agttgctgat tatcccagtg aagctcgaat
     6181 acagcttcgt catcgtcttc atccaaaaaa tcaagcaaat cgctttgccc ttgagctaat
     6241 acttcattta gatattccac taaagaatca gctactgatt ttgccaaccc tttttttcct
     6301 tcataaggga ttactgctag ataattgcgt tcatcaaaaa cagatttctg aggattaaaa
     6361 agaagtacgc cgtcttcaaa ttcgataatt ggttcttgcg aaacgatccc ttctgcatca
     6421 tcaatctcaa attgtgcttt attttctgca aacaagcgaa caacgatctc gatcgtatga
     6481 ttttttgtat cccaatctaa agctacatca tagtctgtca cttgtttttc aattatttca
     6541 tctaaatatg ttaatagatc cttctttttc attttctttc ctccaacccc aatctttctc
     6601 gatttggttt tttctttgta aagagatata aggatttccc gacacaacaa aacgcaacgg
     6661 cttttctgtc catacacctt tgtttgggat accgattcta ggcaaagcat tgatcttttt
     6721 tggtgtcttt tttttctcaa aaactaagcg taacgaacta tctgctatag actgaccata
     6781 aagttcttga ggaattgcta atgcttccac taattttccc ggaccgttgc tgatatctgg
     6841 accagttttt ccgccacgat ttttactcat ttgatcaatc attgcggcgg gttcgattgc
     6901 acgtatcatt actccttgag gaatcccttc tggctgagta atgatgttca aaatccgatg
     6961 agtatgcatc gtgtaaaggt agatcgtacc tggtttttca tacattgccc gcacacgcgg
     7021 tgttcttctt aaaccatagc tatgggcggc ctcgtcttct ggacctaggt atgcttccgc
     7081 gtctacaata tatccagcta atcttccttc tggtgtgtca tgctccaaat acatccccaa
     7141 taaatcccgt gccacttctg gcgtcgtttt attccaaaaa aattcatcta tcttttgttt
     7201 atccgtcatt tttctcacct actttatcat acacaatatt agttggataa gctatgaaaa
     7261 caaaacaaca gaaggctgtt ctttctaggc atcttgctaa atatgatata attaagggac
     7321 gtaaaaacaa gaaggaggaa aatcctatgc aacagccatt ggcttatcgc atgagacctc
     7381 gaacgatcga tgaagtcgtt ggtcagcagc atctagtcgg ggaaggaaaa atcatacgcc
     7441 gcatggtcga tgctcgcatg ctctcttcca tgattctata tggacctcct ggtactggaa
     7501 aaacgagtat cgcaagtgcg atcgccggct ctactaatta tgcatttcgc atgctaaacg
     7561 ccgctacaga ttcaaaaaag gatctacaag ttgtagcgga agaagcaaaa atgagtggta
     7621 cagtgatcct cttattggat gaagtacacc gattagataa aacaaaacag gattttctgc
     7681 ttcctcattt agaaagtggc cgaatcatct tgatcggagc gactactgag aatccttata
     7741 ttaccatcaa tccagcgatc cgcagccgta cacagatctt tgaagtcaaa ccattaacag
     7801 aacaagacat ccagttggca gtcgaacatg ctttaaggga taaagaacgc ggccttggac
     7861 aacaagcaat ccagttagac gaagatgctc ttcttcacct ttcgcgtgca acgaacggcg
     7921 atttgcggag tgcattgaat gggctggaac tggctacact atcgactcct tcagataaag
     7981 aaggaaggat tcatcttaca ctgtcgatca ttgaagaatg tgtccaaaga aaagctctta
     8041 cccatgacaa aaatggagat gctcattatg acgttatttc ggcttttcaa aaatcgattc
     8101 gtggaagtga tgtggatgct gcacttcatt atctcgcccg tttagttgag gcaggtgatc
     8161 tggcaagtat ttgccggcgt ctgatggtta tcggttatga agatataggt ttaggtaatc
     8221 ctgctgctgc ggctcgtaca gtgaacgctg tattagctgc tgaaaaattg ggattgcctg
     8281 aggcacgtat cccactagca gacgtcgtcg tcgatctttg cttgtcgcca aaatccaatt
     8341 ctgcctacat ggcattagat gcagcacttg ctgatattcg agagggcaaa gctggagatg
     8401 ttcctgatca tttgagagat agccattaca aaggtgcgaa atcactcaac cgtggcgtgg
     8461 gttatcagta tcctcatcat tttgatcagg cttgggtcaa tcagcaatat ttaccagata
     8521 aactgaagaa tgctcagtat tatcagccaa aagatacagg gaaatatgaa caggctctgg
     8581 gtcaacagta ttaccgtata aaagagtgga aaaagcatcc tccaaaaaac tagacaagaa
     8641 aacggttctg tttctgcgag ctctctcatt gctcttttta aattcatatg ctactataga
     8701 ggtgggaaat ctgtgatgtt cgaattgtcc tctttgtgtg ttgaccgaac atttttattg
     8761 atatcttggg acccgtcttg tgtcactgct ggtaacatgc cttatcattt ggtagttcga
     8821 agctacgacc atgggaccca cctgcttgaa ttcgcgggat caatactaca ggacaaacaa
     8881 cggcattaac ggagattacc atcttttaaa gaataaagca atttgctctt ggcaaattgc
     8941 tttttctatt tgttgcttat tttggaggat ttgttcatga ttaggatttt aatgattatc
     9001 gccactttag tattattatt cgttagctat tatttattta ataaacagga tatctttttt
     9061 gtattgatca acaaaaatga taaaaatcaa ggattcctac aattttacgg agctgcatat
     9121 gctgttttag gcgtaatggg tattttaaca gcttttttca atcaacgctt tattgccttg
     9181 gtctttttac tgatcgtaat tattgtttct gccaccttca gtatccgatt tgcaaaaaaa
     9241 attgctgaac caaagcagta aataattctc tttttcccta aagtgttcta caataaaaga
     9301 gtacaatacg attgacttga ggagtgaatg cttatgttac aacaatacaa aaaaatcatg
     9361 gttgccgtag atggttctga tgaagctgaa ttagctttta aaaaggcggt caatgtagct
     9421 atccgaaata acggagaact tctattagca catgtcattg acacacgttc tttccaaacc
     9481 gtttcatcct ttgatggaat gcttgcagaa caagctactg aaatggcaaa gcaaacattg
     9541 gcagattatg aaagcaatgc gaagaaagct ggcttgaata atgtaacctc tgtcgtagaa
     9601 tatggctcac caaaacaaat tattgccaga gaaatcccag aagataatca agtagatttg
     9661 attatgttag gagccacagg attgaatgct gtagaacgtt tatttattgg ttctgtttct
     9721 gaatatgtta ttagaaacgc agcttgcgat gtgcttgttg tccgaactga tttagaaaac
     9781 caattgccag ctaaagacca caccaaagct taacacgtag acgacctgcc aactatcggc
     9841 aggttttttt gcacaaaaaa ccgtgataaa aacaaaagac gcggcaagca ctcatcggtc
     9901 tttataatgt ttaaaataaa gggaaaacag tcaatgagtt caagtaaaat aaaaaaaagt
     9961 gaacaccagg gcgattagtc gccctggtgt ggtttaaaac gacctaagca tgccaaagct
    10021 tattggttcg tcaatgtgga agtttccgat gaatctgata cgaaaaaata tgaagtatcg
    10081 atggcggata ccgattttat cgccaaacta aaattttatt tcgggttcgg gtaccgcgag
    10141 aaggcaagtg ctacattcgg taaatcattc aagaattgat gaaaggctac cgcttgccat
    10201 tgcaaccaca acgatactcc tgaacgcgat gttggtgaga tttccaaaga gaagagctgt
    10261 ttaatgcaac ggtattaagc aaatggcaaa aggaagtcat gtagacaaag ttttttatca
    10321 aacaccatat gatttcgcct gaacgctggt ttagctcaga atacaatgat gtggtccacg
    10381 agacattacc ctcagtcaga taaaaaaagc tctctgacag aattacacat cgataaatat
    10441 taagtaaatg tcagcgctag ttgaactttc tgacactttc aggtacactg gaaaagacaa
    10501 ctacacgaag gagggaaaat actatgatgc gcatgactaa aataacaagt aatgaaaatt
    10561 accaacgata ccatatcgca tttagccatg caattggttt tacagttgct gttgacggga
    10621 caagtttgtc caaaacagtt gaattcacgt aaaatcaatg gccatctcaa ggtatttttc
    10681 tcatgataat ctgagtttaa ataacacagg ccatggataa ccatggcctt tttgagtggt
    10741 ctcaaggaga gagatcatga tcatacaagc aatttctatt aaaaaaacaa tcggcgggaa
    10801 tccattattt gaggatttat cctgtcaaat tgaagctggt gaaaaaattg ccttagtcgg
    10861 tatcaacgga actggcaaat caaccttttt acaaatcttg accggcagag aaggtgtcga
    10921 cagcggtgtt atcagtcgca aaaaaggctt aaagatcggg acggtcgaac aagaacttac
    10981 agtttccgaa gcgaccgtca accactactt gctccattct gccgctgaga tacaggacct
    11041 caaacagcaa atgagtcgct acgaagcatt gatgacagaa ccagatacaa acttagagaa
    11101 gtgtctggcc cgttacggtg agctgcagca tcgctttgaa gaactaggcg gttacgtatt
    11161 agaagaccgg attgcagcga tcttgcaagg cttagggatt ccacaccgtc aggaagcacg
    11221 gttaacagaa cttagcggag gcgaacgagt taaagtcgct ttagccaaaa ttttagcagc
    11281 cgatgccgat ttacttttac tcgatgaacc gaccaatcat ttagatctta acagtattcg
    11341 ctggttggaa aactatttac agaatacaaa aaaatcggtt ctggtcgttt ctcatgatcg
    11401 gcaatttctc gatcaagtca ccacaaaaac atgggaactg gaagaaggtg cattgattga
    11461 gtatcccgga aactacagcc gttttcgagt cttgaaagaa gctcgcttgg ctgagctgac
    11521 aaaaaattac gaactgcagc aaaaagaagt ccagcggttg aaagtcatga ttcgtcgttt
    11581 tcgtcagtgg gcacatgaag gagacaatga gtccttcttc aaaaaagcca aagaactgga
    11641 acgccgcctg gcgaaactga cactcgtcaa accgccacct cccccgaaaa atcggctgca
    11701 aagcttgtct aatggtggaa aatctggcaa agaagttttc atcattcaaa acttgcacca
    11761 gcagtacgct gatcaagtac tattcaaaga cagcagtttt gcagtctatc gtggagatca
    11821 tttagccatc atcggcgaca atggtgcagg aaaatcaacc ttactcaaat tattactcgg
    11881 tgaggaaaag ccttacggtg gcaccatcaa attaggtagt agcttgcaaa tcggttattt
    11941 gccgcagcag ctgcagtttg ccgatcccga tagccgtttg ctggcttacg ccatcacatt
    12001 gactggaaac gaagaagtcg ctcgacggca attagccaaa agtggctttt atcaaagcga
    12061 tgtgggtaaa cggatcaaag atttatccgg tggtgaaaaa atccgtttgg cgttgctgaa
    12121 actcttttta gaaaaaatca acgtcctgat tttggatgaa ccaaccaatc acctcgacag
    12181 ctatgctcga gaagaaattg aagaaatgct gcaagactat caaggaacgc tcctagcagt
    12241 cagccatgac cgctactttt tgcagcaaca cttccaagaa gcactcgtta ttgatcagga
    12301 gcagatcagc cgccagccgc tcggccaact gattgactag gacgtttttt cgcaacaaaa
    12361 aacttgattt tcatacctgt gctttcacga ttcaggcaaa gtacaggtgg aaaatcaagt
    12421 ttttattatt cgatcaatga atgtttcgga tcggttttct agctaaacat aaagcgaaga
    12481 ataaagtcga cagcccacga ggctgatcta aaaatgtttt ttctttttac atatgtaatg
    12541 tcccttataa agatgaatga aaataaaata ttgggatttt cctgaaaatc aataaataaa
    12601 atgccagtct tttccctttt gaccactttt ccaagcaaaa ttccctaaga agatcctgta
    12661 aaaaagaagg gaaattgaag ttaaattacc attttgcggt cttctccgca aaaaaaacac
    12721 atgcaagaat tccctcttgc atgcgtttca tcttttatcc taacgaatgc tttggcttag
    12781 ccgcgcagac gctccacatc acgggcaatc attagttcct catctgttgg gatcactaat
    12841 actcttactt ttgattcttt tgtagagata tctacttctt caccacgcaa ttcattcaac
    12901 gctggatcga tttcacaacc aaaccaagac atacctttga tgatttcact acgtacatgt
    12961 ccgtcattct cgccgatacc agcagtaaag acaattgcat caacaccatt catcgttgtc
    13021 acataaccgc cgatgtattt tcggatgcgg tccacgaaaa tgtcataagc taaacgaata
    13081 tgttcttttt catagttctt ttcaagatca cgcatatcac tagacaatcc tgttaggcct
    13141 agcagaccag atttcttgtt caaaacattg atcatttcat caatatcaat ctctaatttt
    13201 tccattaaat atgggagaac agctggatcg atatcacctg aacgtgtacc cattgtcacc
    13261 ccagctaatg gtgtgaatcc catagaagta tccatggatt tacctccatc gactgctgtg
    13321 atcgatacac catttcctaa gtggcaagtg atgattttta gttcttcgat cggtttgttc
    13381 aataattttg ccgcacgttc cgacacataa cgatgacttg tgccatggaa gccatatttt
    13441 cttactttga aatcttcata ataagacacc ggtaagctat ataaataatt ttgctttggc
    13501 attgttgagt ggaaagatgt atcgaataca gccacactca aaatgtcagg taaaatcttt
    13561 ttaaacgctt tgatccccat taagtttgca ggattatgca aaggagcaaa ttctgctaat
    13621 tcagcaatgc ggtccattac tttctcatcg atcactactg aatccccata agtttctcct
    13681 ccgtgaacaa cacggtgccc tactccggtg atttcatcat aagagcttaa aatttgcagt
    13741 tcaatcaact gatctaacag catttttact gctacttcgt ggttttcgat atccatgatt
    13801 tgttcgaatt tttgattatc gccgtacttg atggtaaaaa tagaatcttg taaaccgata
    13861 cgttcgacga tccctttcgc aactacggtt tcttctggca tttgatataa ttgccatttc
    13921 aaacttgaac tcccagcatt gattgcaatt gttttagaca tatgactctc cttctcgttg
    13981 aatgattaca gagcagatga tttccagttt ttaaactcgt tgaaaaattc cgtcaccttc
    14041 tctggttctt ttaatgaagc aagtttcaca agcaagactt cttttacttg ttgtgcttgt
    14101 ggtccttttt tctgtaagat caaaatactt ttttgcgatt gtttattacg gaacaattca
    14161 tctggcaatt gaatcattcc ttgaagataa ccttcttctt taaaccattt tttgatttct
    14221 tcactttgat ccgtttctaa aaatccactc ggcatcaaaa acaaaccaaa accatccggc
    14281 ttcacatatt tcattgattg ctctagcaat aaatgatgtg cataactatg gccttcttcg
    14341 gtacttgtta aaaactcttt tgctttttcg tcattcggat aataaccgat tggcaaatcg
    14401 ctgattgcaa aatcgacagg atctattaat aattcctgca acccgtcttg atgaaagtat
    14461 tgaacatttg cttgtgtcaa atcacttgta gaagctgcga cagccaacaa tgtatcatca
    14521 atatccacac caattccttc tgtttggtac cccgcattcg ataggtttaa aagaactgtt
    14581 aataacaagt tgcccattcc tgccgcaata tccagaatct taaccggtgc ttttttattt
    14641 gtgtatagtt gttcaataag aaaaacaaat aaaaaaccga tactatcagg agtgagttga
    14701 tggttcgcct gcaaatgttc tgtctggctg cccttcaata aaagtaattg agacagtctg
    14761 cgccattctt ctggctcgaa agaaagtttc ttcaactctt cataaagagc tgtgatccgt
    14821 tgcgttgtct cttttgtcgg cactccatcc accacacgta cttgataatc atctattaaa
    14881 ttttctgcat tttcgacata ggcttctaaa aaggaagttc ccaatgcatt ctgtaaaagc
    14941 tgaatagctt ccaggttttg attaaaccct tgttccattt tttctggtaa catggtgcac
    15001 ctcatttcta gatatattat aacaattttt tcacctgttt aattctatcg aacaattgac
    15061 aaaatttcaa tcgttttcac tgttttttat ttgtgaaacg gcaaacattt ttttattaaa
    15121 aacagtgtta taacagaaaa gagggcgttt tcacgaacat tccatcattc tgtactagta
    15181 tctgtatcat tttttacttc ttctgtgaac cgaaagataa acggtgagac ttccacctct
    15241 aagaataggg actgttgtac ccactgatat gttacagatc cagttgtgta gtaaaaaact
    15301 ccttccgaac tttcggcaag cgtagactgt tccgataaag ccatctgctt cattattttt
    15361 gctatataat aatttttgat tcgtatatta agatccattg acaaacggta gctttctaat
    15421 gctgccatca ataaaaacgt acataaaaga aataaaagca agacagaaaa taaaatagag
    15481 cccttataac attttctact gatcgttttt tgtccatatg gcatatccat cttctccatt
    15541 ttcaaagtgt acataaaaag aaattgtgtg ttctttttcc aataattgaa gctcagtgat
    15601 acttgttaac ataggttgat gtccgccgga agttcttctt ttacgtatca ttgcctgata
    15661 tctttcgatg atatactgtt tatcctcgat cgtgtagtgc agttgctgat ttttcacaga
    15721 gacattgtga ctgctcttga gttcttcttc taattgtgct aaaaatatta gccactcttt
    15781 ttgatcttta cgctgcaaaa attcacttac ttggatggtt tgctgaatca aaagattgaa
    15841 tgtgagcaat agactgctca atatcatcag tccaattaga cattcaatca gcgtaaatgc
    15901 tggaattact tgttttttca atactgatcg cttctttctc catattgaca aacacttttt
    15961 cgattactcc attattatcg acaatttgga tatcgtatgg gatcatttct tcccttcgct
    16021 ctggtctttt gtgatacatc cgctgcgtct tgatatcttc ataaatcatt cgaataaata
    16081 gcagttcctc cccacttttc ttttctgcct gaagcaaaaa cagctggcca aagctcacta
    16141 agtagacaca gacagaagcg atcataagcg ctgtaaggga ctccaacaaa acaaatcctg
    16201 ttttattcaa ttctttttgt atagcgacca ctccctatct ggaattgata cgtaatcgtc
    16261 cgctcttttt cttcccaata aaattgataa gcttttagtg aactttcatt cccggttcta
    16321 gcagcaaaag taatagaagc atggcgtttc aaggtaatct catcaggaat atctaatact
    16381 tcccaattta ttttatctgg attcggaaca tgaaaaatga tttgattctc ttcactattc
    16441 caataaaagc ctgtttggat ctgattcaca atagcgattt tttgcgtaga atagattcgt
    16501 ttttcaaatt ggccgaaaaa ttgatagact gccaattcat tcttccatgc agaaaatgcg
    16561 agaactggaa aagaaaggat cgttgtaagt aataaaatca gtaagagtga ttcaaacaac
    16621 gtatatcctg atgctaaaac cttaccttca aggccgctga tagatttctg catgttctcg
    16681 tttgatatag ccttcattca acaattcctc cacagtagga actcttccat tattttgaag
    16741 cgcatagagc tctttttgtg tgtctaccaa ctggatgatc gcttcacgac tttcttggtc
    16801 tacatgattc ctataacgcg acaaattagg gacgaacaac aacactaata cactgatgat
    16861 cagtaagacg attagcattt cgatcaatgt aaaaccgcta taataagata tttttctctt
    16921 tttcatggca aaacctcctc tatgcttccg taaacaggta ataaaacagc ggcataaact
    16981 aataaaatca aagccgctac gcccaaaaaa atgattggct gaataaaatg catccagtga
    17041 tgaagacgtt tgaaaagctg ttttcttgtc cattcgctgt aaaaaatcag ttctttacct
    17101 aaacttccct ttgcctctcc ctgtaaaaca attttactga attcttctga tagaaaacga
    17161 taattctgga actgctcagc caatgacata cctgcctcca atccttgaat caacttagag
    17221 gcaagatgct gaatcagact ttcttgtttc gtttccttta agcagactat gatttgcctt
    17281 aattcgaaac cttcatgaaa aagctttcca atctctagtg acaaataagc cgattgatac
    17341 aaggaatata tcggtccaat caataattgt ctgcttatcc attcactctg atcaacagga
    17401 gacaacttca tcagacgata ccttactagt aaagccaata gagtaaccgc taggatgaat
    17461 agtagaacat accaatgaaa gacttgtatc agctgaatac cccaatgctc ttgcatgacc
    17521 attcctgaac caataagctg aggcaaaacg aacaaccgca tactaaacaa gatacctagc
    17581 aaaaaggtga gcaatagcat cggatatgca agcagctttt tcaaatcatt gcgaaatcct
    17641 tcaacaagcc gaaactgttt ggcaatttcc aacaaagttg tcggcagatt cccatgacgt
    17701 tcagctaact cgatctgtac cagttgctct gctccatatc ctatttgcga aagtacctca
    17761 ctaaacgctt gcccttcctt tagctgatgt tggatatttg ccaaaataac aggtggaaac
    17821 gaacggatcg tgagtaataa ataaagggat tcttgcagac taaaaccaga taaaagcagt
    17881 tctcccatgg tttgtgcaaa ctggttttgc tggcttcttg ttaatccttt agaatagttc
    17941 tgactgccaa atattttcct gagcttcttc ataactttcc tcccatgatt tttttactga
    18001 ttcttcctcc ataaattgat aactccataa aacagaagga tggtgattgg catcttccaa
    18061 aagttcttga taaatgacac cagctaaaca ttcagatagc tcttcttttt tctttgtcag
    18121 ttcatggatt ctgaccaatg taccttcaag attgcgtgca tgtacggttg caaaaacccg
    18181 gtgccctgtt agtgctgctc ttaccgcagc atgtgcagtt gattcatctc ttatctcgcc
    18241 aatgattagc aagtctggac gatgccgtaa tgcgagtctc aaaagtgtat cataagtctg
    18301 tgctattttc ggattgatct gtagctgcag aaaaaacggt tcttctatct ctacaggatc
    18361 ttcgatagta atcacttgca atttttcttc caaagccaac cgatacatta gagaagtctt
    18421 gcctgatccg actggtccgc tgaaaaggta taatccacgt ctgacggttt tctgtaaaat
    18481 agttgcaaaa tctcgagaac taaagcaacg atggggtgtt tcaaaagtac cgtataatag
    18541 ccgaatcact aaactctctc gttgaaggta atctcctaca gaagacaacc gcagacgaac
    18601 ttcctgtttt tctaaagaat aagtaaatgc acctaactga gcttttcttt tttctccgat
    18661 atccatctgt cccaaatact taaatcttga gatcagttgc tgtccatctg ccgaagagac
    18721 tttcttccag aatgttcgtt tttttccata gcgaaaatag atgtaaaata cttctgaagc
    18781 tggcaatata tatacgtctt gggcatgttt ggcaaaactt tcacttatca tttgatccga
    18841 taatcgtttt atttccatta ttctcccctc cacaaaaaac atacgtcttt ttgatctttt
    18901 tgtttcaaat aaaatgaatt tacaaaaaaa tattggcaaa aaccgaattt atgttatact
    18961 tataaaaatt tatcttggga ggcactccat tggaaacaac attaaggaaa tatatcgaag
    19021 tagcaagtaa aaaaaagcct gctgacctag tgatcaagca tgctaagatc gtcaacgtct
    19081 ttacgaaaga aatcatggaa aaagatgtag caatctgcga aggaatgatc gtaggaatcg
    19141 gtgaatacga aggaaaaagc gtacataatg ctaatgggca atatctagtg ccaggattca
    19201 tcgatggcca tgtgcatatc gaaagctcac ttttatcacc aagcgaattt tcaaaagtat
    19261 ccttgcttca tggagttact actgtagtga cagatcccca tgaaatagga aacattgctg
    19321 ggagtacagg tcttgagttt atgatagaag atgcaagaca aacactcatg aatatctttt
    19381 ttatgctacc ttcttgtgta cctgtaaccc cttttgagac caatggtgca aatttaaatg
    19441 caaaaaaatt agcccctttt ttacaaaaac ccgaagtttt aggcttagct gaagtcatga
    19501 actatcaagc agtagcaaat aatgaaacag acatcctaga aaagatccaa ttgatgcacc
    19561 agcataagaa aaaaattgac gggcatgcag ctggtatcgg catggaagag ttaaatgttt
    19621 atcctgcagc aggtattcgg acggatcatg aagctacaac tgcaaaagaa gctaaagaac
    19681 gtttggatct tggtatgtat ttaatggttc gagaagggac cgttgcaaaa gatctggcat
    19741 cgctactacc ggcgatcaca cctgaaaatt caagaagatg cttctttgtc accgatgata
    19801 aactgatcaa tgatttagta aaagaaggat caatcgatca tatcgtccgt caagctattg
    19861 cacttggaat cgatccatta caagccattc agatggcaac attgaatgca gcagaatgtt
    19921 ttgatttgaa aacactaggt gcgattgccc ctgggtatca agctgatttc ttcttaacag
    19981 acgatcttac caagctgcca attcatgaag ttttctataa gggaacatta gttgttcaat
    20041 ctgggcaact ccaagaccat ttatttgctg atcattcaaa ttcatatacg tgttgtctgc
    20101 caaaactaaa cgctcaacca ttaaatccac attcgttaga gctgcttctt tcttcttcac
    20161 aagcccatgt gatcgaaatt atcccgaata gtctagtgac taaagatttg gtagaaaaag
    20221 tggaccgaca aggcgaacga ttcattcctt caatcgaaaa agaccaatta aagattgcgg
    20281 tgatcgagcg tcaccatcaa acgggaaaca ttggattagg catagtgaaa ggatttcagc
    20341 tgaaacaagg agcgatagcg acaacgattg cccatgactc gcataatcta gttgtcgttg
    20401 ggacaaacga cgaggatatg ctgtatgctg ctaatctttt gattcaaaaa ggtggcggga
    20461 tgaccgttgt caacaaacag caggaaatag cttgtgtttc tcttcctata ggcggaatca
    20521 tgacacagga acctttttcg atcgtctatg aacagttgaa tgaattaata actaaagcac
    20581 atcaactagg agcagctaaa acctttgatc cttttttgac actttctttc ttaacattat
    20641 ctgtcatacc cgaattgaag atcaccgaca aaggattatt ttcattttct cgcttccaat
    20701 tgattccacc ttgtggaaaa accgatgaaa taaatacatt ttaaatcaaa taaatcccag
    20761 aaaatacatt acatctgtaa tgttcatttt ctgggattta tttatggata ttaatgtttt
    20821 tatgaaagca gaaagttagt ttcacagact tttctttttg gtcttatctt gaaattgagc
    20881 ttctactcga taaatacgtt gaccttttga agaaaatttt tcttcatact ccgtcataat
    20941 attgccttca aattgacttt gatgtaaatc taaccatacc tgtttgagga tcatgccata
    21001 ttgagaaaaa cttgccagag aatactcaaa caatccttga ttatctgttt tgaaatggat
    21061 ttctccattt ggacgaagaa tctgttcatc tacagcaaga aaagtcttat atgtcaatcg
    21121 ccgtttttca tgacgttttt ttggccatgg atcggaaaaa ttcagataga tttgatctac
    21181 ttcgctgtca gcgaaatact ctgtcaacgc tgatccatcc acatgtagaa gtttcaggtt
    21241 aggaagcggt tgttcgatca acttgtctag tgcaatcgaa acaacactga cctgcatttc
    21301 tactccaata taattgattt caggatgggc ttttgccatt ccagtgatga actggccttt
    21361 ccccatacca atctcaatgt ggattggatg atcgttttca aacaattcat tccattttcc
    21421 cttccacaat gttggatcac taattacaaa attcgggtga gccgctaaca tttccgctgc
    21481 tcccggacga tttcggacac gcattattta tctccattcc tttcctctaa ttaaaaaggt
    21541 ctggccaaat ctccgcagag tttgggccaa acctcagcca ctgaaaattc gttttaattg
    21601 taaaatttct tcgttcatcc gtcgatgatc ttcacgtttg caatatctgg tgatttcttg
    21661 gagcaaattc atggccgcat accaatgaaa tttttcaagc atttcgtcag tcgggcgtat
    21721 gccataactg atcagccact gattccagct agacagtggc acataatttc ctaaaatcgt
    21781 tgcaatatcc accgcagggt ctgcaaacat gattgaatcc caatcgacta gatataaata
    21841 atttttacat actaaccaat tacgatggtt tacatctccg tgtactactg ataagttttc
    21901 cttatctatt gacgggatct gctcacgaag aaaacgatgg acaatttgca gaaattgatt
    21961 tttctggagt tcttccggta attctttttc atatttttgc agcatcttct ccggcgtcat
    22021 gacacgacca ccgatacggg tcagcatggt tttaagagaa tttgaatgat gtaaatggta
    22081 caaaacatca atcacgtcgt ttctttgacc aatctcatta ggagtcagca agcgaccttc
    22141 cagccactct tgtgctgtca aagtatcgcc attccccgtt cgttttgtcc aaacaagttt
    22201 tggtgtaatt ccttcccttg aaagagcagc caacatcgga gtcgtgtttc gtttaataaa
    22261 gactttgtcc tcatcacgca tgcctatgta tgtttttcct gtgtcgcctt tgatgggacg
    22321 taaccgccac tcttggtcta actgaaactc catcatgcat tctcccgtcc tcttactctt
    22381 tgttttgcag tacttattat tctagtcgcc tgaacactac acgtcaagca catatacgtt
    22441 ctttttcatt taattgccat tcaaaaaacc agcttttctc aaaaaaaata gaacgaacag
    22501 cattcttact gtcaacgctc tatttttcat tctcattttt accctacaca gctttgtttt
    22561 cagtctgaca tcttcttcaa acgagttggt acatatagtt taacaaagag catagtaaca
    22621 atcaaataag ccgctagcgg aacccatcgc tgctgcccat ctaatacgac tgcagcaatc
    22681 aaaccaaaaa taaccgacgc gataattaat acccatgtga gtagatactg aatcgcctgc
    22741 ttcttctgat tcttatggat tggataaagc tgaacgagaa ccatatagcg gaactggttc
    22801 gctaaaggta acagttggaa accgatcaaa tataaaaata agaaaccgat aatcagacta
    22861 aaataaaagt cgactactcc ggcaatcaat aacccaccta taattactaa tcggaaataa
    22921 agaccactaa aatcagaacc tcttgccaat cttcttgcaa acaagtaaag ataagtgttt
    22981 tttgattctc ttttaatatg agtcaatata ggatccaaat agcgtcttct tttgatcttt
    23041 gccgtaattt caggaacatc tgtgaataaa ttgataaatt gatagatctg atgcaaccta
    23101 tgttcttcta catgcaccat tttctcccaa tccaatcctt tttcttgttg cttccacaaa
    23161 aaccagtaat atagacttgc ctgtactaat gcacctgcta ttcctgccca tattagccaa
    23221 taaaatgata gaaacaaaaa gatcagagaa gaaaaaaacc agcctaattt ccaataacga
    23281 ataacttgtt gatctagtcg aaacaattct gctctcttga tttgcagatg actgaatttc
    23341 aaactccata atatgatcag atagacgaaa aacatcgaaa aggattgctc cgtactaacc
    23401 accactaatg gcatggccaa tccgcatgcc aatatcaaaa agatcaacgg aaaaatgcaa
    23461 gaatagcgaa aagctcgttc caaataggtt tgcatttctt tttctttagg aagtaaaaaa
    23521 acaatatctg ctgcctctgc aaaagtagcc atcttaccca cgaataaaca gagcatccaa
    23581 aatacagaga ttatgagccc tccaattgca aacggtggct gcagggtttt caaccaatta
    23641 gaataatata atcccacacc gccaatcaag aaaagacaaa ctaaaacaaa atgatcgttc
    23701 aatacataac gcatatattt catcattttc ttttgatgtc ttgctaaacg tttttgaaaa
    23761 aaactactca tgtgcctgct cctttgttaa agcaatatag atttcgtcca aagaagaatc
    23821 cggcagctga aattcttttc tcaacatctc aagagaaccg tctgctcgta tctctccgtt
    23881 atgtaagacc acaaaacgat cacaatattt ttctgcagta gctaaaatat gcgtagacat
    23941 caaaataccg gctccttctt ttttcatttc taccatcaat tcaagcaatg cattgatagc
    24001 tagtggatcc aagcctaaaa atggttcgtc gatgatatat aaagaaggct cgatcaaaaa
    24061 ggcgcataag atcatcactt tctgtttcat tccctttgaa aaattagctg ggaaccagtc
    24121 aagacgatta gataaccgaa aagtctccaa caacttttct gctcttttca ttgctatatc
    24181 cataggtata tcgtaggcca tcgcagtgac ttcgatatgt tctctcaatg tcaattcttc
    24241 atataaagaa ggagtctctg gaatataccc aatttgtttg cgatattctt ccgtattttt
    24301 ctttaaggat agatgattga tttctatcgt tccttttgaa gcttccagca gaccgataat
    24361 gtgcttgatg gtcgtacttt tccctgctcc gttcagtcca atcaacccta ctatctctcc
    24421 aggctgtaca tcgaaactga tatcttttaa cacagggaga tgtccgtacc cccctgtgac
    24481 atgatcgata attaaactca ttctttttcc tccgccattt atttttattt attataccac
    24541 gaagaaaatg acggtatttt gtttttttcc ttacaatagc tttaacttat ggtaaagtta
    24601 agctgaagta tttattcttt atagaaagga tgttttatat ggaaaactgt attttttgca
    24661 aaatcatcaa tcaagaaatc ccaagctata aaatatatga agacgacaag gtctatgctt
    24721 ttttagacat ctctcaagca acgaaaggac acacattagt tgtgcctaaa cgtcatgtag
    24781 cagatatttt tgaatatgat cccgaacttg caggagaggt tttttcgcgt gtgcctaaga
    24841 ttgctcaagc attggaaaaa gcttttccag aaatgaaagg tcttaatatc ttgaataaca
    24901 accgtgaact agcctatcaa tctgtttttc attcacatat ccatttagtc cctcgctaca
    24961 gcaaagagga tgatttttct attcactttg tcaatcatca agatagttat ggatcggaag
    25021 aactaaaagc tattcaagaa acaatcgtaa agcaggtgtc ctgcgatgat taaaaacttt
    25081 ttcaaaggac tgttatttgg agctgctgct ggcactgtcg gaggtctgtt attggcgccc
    25141 aggagcggga atgaaacaag aaagaaactg atcgaagaat tggaagaagc aacaactctg
    25201 acaaatgatt taaataacag cctgaatcat tttaaaagcg cattgatcga aacaaaagaa
    25261 acagctgaat ctgtgattcc tgctttccaa gaagcaatca aaaaagatat cgaagatttt
    25321 aaattccaag ccgaaccacg tatcacacaa atcaatgaac aagtagaaaa actaaaagct
    25381 gatctgccag agtatcctga acaaatataa taatgattat tttctcaaca gatttaacta
    25441 tataagtgag aaaagtttgc gtatttccta ggaatgtgat gaaattttga attttgtttg
    25501 aaactaccac gacaacgttt ttaaaggaaa tgggatatgg tatagttaat atataaaaat
    25561 aataactaat tacaggagtg caaattttaa tgaaaaaaaa atcaattatt ttggcagcta
    25621 ctagtgcgtt ggctgtttta actctagcag cttgttcagg cgatactaat aaagatatcg
    25681 caacgatgaa aggcggaacg atcactgtat ctgatttcta tgatgaagca aaacttgaat
    25741 catcgaacca atccttagta caacggatga tcatttataa agtattcaat aacaaatatg
    25801 gcgataaagt aaccgataag caagtggatg cagaatacga caaacaagcg aaatcattag
    25861 gtgatacttt tgaatcacaa ttagaagctg ctggatatac gaaagatact tataaagaat
    25921 atattcgtaa caatttagct tttgaagctg gcttgaaagc tcacgtagat attacagatg
    25981 acgatttgaa aactgcttgg aaatctttcc atccagaagt agaagcacaa atcatcaaat
    26041 tatcaagtga agatgaagca aaagatgtga aaaaatcagc ggatgatgga gatgatttct
    26101 ctaaattagc caaagacaaa tcaactgata cagagacaaa agaagatggc ggtaaagtta
    26161 aattcgactc aacgacaaca actattcctg cagaagtaaa agaagcagct ttcaaattga
    26221 aagacggtga gatctccgat gttattacta caacaaatcc tacttcttat gcgacagaat
    26281 actacgttgt caaaatggtg aaaaaccaaa ataaaggcaa tgacatggat aaatacaaag
    26341 atcaactaaa agatatcgct actgaaacta aactaagtga caatgcattc acaactaaag
    26401 taatcggtga agaattaaaa gatgcaaatg taaaaatcaa agatgatgcc tttgaaaatg
    26461 tgttgagtgc attcacaaca acttcatcaa gtacgaaaga ctcaagtgaa actacagctt
    26521 caacaaagag ttctgacaca aaatcaactg attcaacaaa agaatcttca actgaagaaa
    26581 caactgattc atcaaaataa aagaaaacaa aaaaagcgaa gacttatgtc tcgctttttt
    26641 tgttttgaat gaaaaaaata aatttttgct ataaatcttt tggtacatag aaacttcggt
    26701 tgtccatacc gaaaatccga tcggtgtatt ttcctggttc tgtttgacga atcgaagtca
    26761 acatcatctg catcgaagca tcgatattgt cgatttgatg taatatttct gcttccatta
    26821 ttcttggacg tacaggtgag ccatattcta ataagccatg gtgtgctaaa accatatgac
    26881 gtaatacgag aacatcttcg tcagcttcgc tgattttcaa cgcaagacag gctttagtta
    26941 tttcttcgtc aatcaatacc agatgaccga tcaaatttcc tgcaagcgta tattctgtcg
    27001 acataggacc ggaaagttca atcacctttc ctaaatcatg caaaatgata ccagcataca
    27061 acaatgctcg attcagctca ggatattcat cggcgattga tttccctaag tgaagcattg
    27121 tcaaagtatg ataagctaat ccactggcaa atgcatgatg gtttcttttt gctgccggaa
    27181 actcaaaaaa ttgtttttga tatttattaa gtagatagcg aacaatacga ttccaatgcg
    27241 cattggtgat ctcaaacaac aattgattga tttcttcttc catttcttct ttcttcaaag
    27301 gggctctttc catgtataaa ctaggctctc ctggctcatc tacccttgtt aagcgcatat
    27361 gaagaatctt tacttgaggg tttccttgat aaacctctct tttaccgttt agaaaaacga
    27421 ctgtgccggc tgtaaattta ttgatttcct cctccgaagc atcccagtat ttaccatcga
    27481 tagtacctga agtatcttgg aaagtaaaag caataaattt ttttccgttt ttagcgattc
    27541 ttacatctgc acttttgatc aggacaaatt gttcaaactg ctcatcgatt actaaatcac
    27601 gaattttttt cattatttgc tccttctaat tgttttacga tttggttcat ctcatggtaa
    27661 tagcttacca tttctttatc agaagaaaaa caaattattt gatacttttc tccaaattta
    27721 gctaaaagtt cagctaattg atactttcgc tgatgatcat aatgaagcca gccatcatca
    27781 atgatgatcg ggcatatcga acggttagct tctaatgaga ggtaagcaaa tctcatcgcc
    27841 ataatcagct gatctttcgt acctgtagat aactcataga tgggaaattc tgtatatccg
    27901 tctgttaaag tcagtgtatc atttttcagt aatatcttct gataatttcc ttttgttaaa
    27961 atacctaaat aatcacctgc tgctcttaac aactggggta attgtctttc actcatctct
    28021 gttgaaagat cacctaagaa tgtcccggcg atttcaagtg ctccccattc ctccagtaat
    28081 tcctgtaatt ttgctttttg ccggctagct tgttgataaa gttcgtccaa tgtgccgtct
    28141 ctttgcaact cttctacttg aagttggagt cgttgccgat tttcacttgc ttctttttct
    28201 tcttgctgga gttgggattg cttttctttc agttggcgca gtcttacaga taatttctct
    28261 tttgtcagct cttcaggaaa tagtggagta agcatttccg ataattcatt gtatcttttc
    28321 atctgctgtt cggcttgttt ggctttttgt aaaaagacag gtacttccga aggataagat
    28381 aagcctgctt gagtcaattg ttcgctgtat tcttgtatcc atactttttg tttctttttc
    28441 aattgattga tttcttgctt caataaagta ttttgctgat agcttctggc aaatttcatc
    28501 tgttccatct cttgtataaa acgggaaagc aataccattt tttctgaaat tgtcttatct
    28561 tgtatcggta accattcttt aacaaactca aatttttggt cgatttgtcg tagtttttct
    28621 tgtatttcct gttctctttt atgtttccgc tcaaatacgc catataattt ctgataatct
    28681 atgacttcct gtccatattc tttcatgagt ttgagcgtat ccatctttcc taagtgccga
    28741 tgatccgcaa aacgatatac ttcttgctga agaatctgtt tttcttctcc agttctcttt
    28801 tgtttttctt ctgcttcagc taattgagct aaggctagat caagttgtcc tagtttttct
    28861 tgccacattc cttttatctc agagaaatca tttttagttt taatttttgg ttgccttttg
    28921 tagaagaaat agccaatcat gcctgcacca attagtaaaa ggatcacttt caagggcatt
    28981 ggtacaaata tacctgcaat gattattgct ccgccagcca tcagcatata cggtttagat
    29041 gaagagactg gtgctatctc tgactgattt tgtaataatg ctggataggt ctcttccaag
    29101 tcattcactt ctgcttcaat catctcttgc tgttctttag ctatttggag acgcacagaa
    29161 atctggcttt ctagatcagc aatccttctt tcttcagcca gaagatgctt ccattcttct
    29221 tcatataaaa attcttccgg taacggattt ttctgccaat gccacctttg ttccaaaatc
    29281 ttcaattctg aatttgtttc tctagtctca atttgtaatc tttgccattc ttcgtttaat
    29341 tgttccgctg taaattgaag ttgctggaga tcttttagtt cctgctcctg ctctaaataa
    29401 aaataatatc tggctgactg ctgatccatc ccgctatgct tttccagtgt tgcttctaat
    29461 tcttccaaac gctcatttgt ttgctgatac cgtttggaga aagactccag atctaaaaga
    29521 agttgctcat caggaagacc tgtagtgctg ttttttttga gagattgcca ttcttcatat
    29581 aaagaaaaat taagttcttg ctgtctggct aatgaaaatt gtttgttcgc ctcttttaat
    29641 tgtgccaata actgctgctg ttgcgactcg tattcctgtt cttgttggat caacttgcca
    29701 aaataagctt cctgttcttg tttttgatga attttttctt gcagctcttt ccattcattt
    29761 aatttttgat tgattagtaa tttttggcct tttttcttga ataattgttg tgctttctga
    29821 taatattctt gccgcttttg gaatacttga gaactgcccg tgatgcctaa agaaatcagc
    29881 gcatcatgta gctctttttc tctcaaatga tctagttcca tcaactgctc ttgctggaaa
    29941 gtaaatacct gctggaataa ctctttcgtc aaaggtgcga tcagtttttc tagtaactca
    30001 tcacttccca cttgatcccc cagccacacc ttcgattttc cgcgattttg ctgcttgtag
    30061 cgctcgacag caaattcacc atatacagga tgtttaagcc atattttgcc gccataggct
    30121 gagccgtctt ttggtgtata atcctttttt ttacggcctt ttgaagggaa accaaataat
    30181 attgcttgaa taaattgata aatcgttgat tttcctgctt cgtttccccc atagatcagc
    30241 tgattgcctg aataaaagtc aaaacttttt tgacgccatt ttccaaaagc agcaatctcg
    30301 atccttaaaa tcttcattcc tgatcctcct caaataaaaa atcttgatta agcagctgct
    30361 tggctttttc aagtgtttct tcttgatatt ccggcagttc gctcaatatt ctagccgctt
    30421 cttgatgact atatacttca tttaaaattt gttggaaatc ttgaggtgtc tgatagtatt
    30481 gaaataactg ttccatcaat ttagcagaag cagcaagtgg ggtcttcgta taaaaagtat
    30541 tctctatgag agaaatacgc caaataaaga aatcatttat cctatctgag aattcctcta
    30601 acaaatagtc taatagctct cctgattgta tccgttccaa aacatctgta ttgagatggt
    30661 cataatcttt taattttatc tcaattaaac taaatccatc ctctactttt gaaagctgct
    30721 gacgaatgtg aacaatgacc tcttttgtcg ttcttgcttt cctcaatgat atttcctttg
    30781 tcttccaata gacctctgct actttaatcg gatgtatttg gcaacttcct ttacttaatt
    30841 caactagtag aatagatgta gaagcttgtt cctttttagt atgaccttga ggcgcacctg
    30901 gataaacaat tgtttgcttt tcatttaaga ccgttggcac atggatatgc cctaaagccc
    30961 aataatcgta tcccttttct tgcatctttg agggttggaa aggagcatag tttccttttt
    31021 gagagacgcc aggttccccg tggtacaacc caatatggta atctgtgagt tctctagaag
    31081 gaaattccat cactttgtct ttttgaatcc acggttgacg ataactaaat ccactgagag
    31141 agacagtttc tccacttttt atcgttattt tttttgtctc taccgtttcg gatgtaaata
    31201 aatggacatt tttaggaaag gagaaccaat accgctcttt ttgatagaaa tcatgatttc
    31261 cgaatattat aaatacaggt atatggttct tttctaaccg tttcatctgt tctgaaaaat
    31321 gtttctgtat ttttaatgaa ggacgatttt gatgaaaatt atctcctgca agtaaaacaa
    31381 aatcaacgtt atttattatt gcctgttcaa tgatatttga taaaacggtc agattcgtct
    31441 tcaatagttt ttcctgtaca gttttatcaa gagttgtcag tccttcaaat gaccgatcca
    31501 tatgtaaatc tgccgcatgg ataaatttca ccattaaaaa tttcctcctt tttttctttt
    31561 atcataccat tttcaatgtt ttttttcatc aataaccgaa aatttgttcg tattatgcaa
    31621 agaaattacg ataaatggcc gacttcaaaa aataagttta ttcgaaaaaa agattgtgac
    31681 atgatttttg tcacaatcct aaatatctat gttatattcg cttcgtttga cactgaagaa
    31741 gtactatttg tactggaatt tatgctccat ttttccgtat ttagaattct atgttctata
    31801 ctaccttaat tagtttatat ggtaagaatt ctttcaaatg cttgagatac tccgtcttta
    31861 tcatttgaaa cagtgacata atcagctttt ttcatgattt cttgatgact atttcccatt
    31921 gcaaaactaa tatctgcact ttcaaacata gataagtcat ttaaactatc tccgatcgcg
    31981 attctcaaag gtttctttaa attgtagatt gattccaaac gatcaaacac cacaccttta
    32041 gatacatttt gaggtacgat ttcaatattt acaggtgccg attcagtaac agacacattt
    32101 agcatctttt tcaaatggtt aaatatatta gctttcaatt gacgattagg aataaaaata
    32161 aaaaatttgt aaatgctgat agcatccttt tctataattt cctcaatatt attataaggg
    32221 attgaacaaa tagatttttc atactcaagc tcatagttta gctct
//
LOCUS       DAZULG010000020        29754 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000020 DAZULG010000000
VERSION     DAZULG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.020"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(189..929)
                     /locus_tag="ACG9CY_000858"
     CDS             complement(189..929)
                     /locus_tag="ACG9CY_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937388.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1059..2006)
                     /locus_tag="ACG9CY_000859"
     CDS             complement(1059..2006)
                     /locus_tag="ACG9CY_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4937389.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2135..2899)
                     /locus_tag="ACG9CY_000860"
     CDS             complement(2135..2899)
                     /locus_tag="ACG9CY_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4937390.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2903..3292)
                     /locus_tag="ACG9CY_000861"
     CDS             complement(2903..3292)
                     /locus_tag="ACG9CY_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937391.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3427..3969
                     /locus_tag="ACG9CY_000862"
     CDS             3427..3969
                     /locus_tag="ACG9CY_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS4937392.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4117..4749
                     /locus_tag="ACG9CY_000863"
     CDS             4117..4749
                     /locus_tag="ACG9CY_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937393.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4883..5008)
                     /locus_tag="ACG9CY_000864"
     CDS             complement(4883..5008)
                     /locus_tag="ACG9CY_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS4937394.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5142..6035)
                     /locus_tag="ACG9CY_000865"
     CDS             complement(5142..6035)
                     /locus_tag="ACG9CY_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS4937395.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6217..7143)
                     /locus_tag="ACG9CY_000866"
     CDS             complement(6217..7143)
                     /locus_tag="ACG9CY_000866"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS4937396.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7228..7989)
                     /gene="pflA"
                     /locus_tag="ACG9CY_000867"
     CDS             complement(7228..7989)
                     /gene="pflA"
                     /locus_tag="ACG9CY_000867"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS4937397.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8233..10464)
                     /gene="pflB"
                     /locus_tag="ACG9CY_000868"
     CDS             complement(8233..10464)
                     /gene="pflB"
                     /locus_tag="ACG9CY_000868"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS4937398.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10741..13191)
                     /gene="parC"
                     /locus_tag="ACG9CY_000869"
     CDS             complement(10741..13191)
                     /gene="parC"
                     /locus_tag="ACG9CY_000869"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS4937399.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13203..15248)
                     /gene="parE"
                     /locus_tag="ACG9CY_000870"
     CDS             complement(13203..15248)
                     /gene="parE"
                     /locus_tag="ACG9CY_000870"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS4937400.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15414..15848)
                     /locus_tag="ACG9CY_000871"
     CDS             complement(15414..15848)
                     /locus_tag="ACG9CY_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS4937401.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15975..16613
                     /gene="plsY"
                     /locus_tag="ACG9CY_000872"
     CDS             15975..16613
                     /gene="plsY"
                     /locus_tag="ACG9CY_000872"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS4937402.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16745..17620)
                     /locus_tag="ACG9CY_000873"
     CDS             complement(16745..17620)
                     /locus_tag="ACG9CY_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4937403.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17702..18493)
                     /gene="codY"
                     /locus_tag="ACG9CY_000874"
     CDS             complement(17702..18493)
                     /gene="codY"
                     /locus_tag="ACG9CY_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS4937404.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18511..19911)
                     /gene="hslU"
                     /locus_tag="ACG9CY_000875"
     CDS             complement(18511..19911)
                     /gene="hslU"
                     /locus_tag="ACG9CY_000875"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS4937405.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19925..20473)
                     /gene="hslV"
                     /locus_tag="ACG9CY_000876"
     CDS             complement(19925..20473)
                     /gene="hslV"
                     /locus_tag="ACG9CY_000876"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS4937406.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20510..21415)
                     /gene="xerC"
                     /locus_tag="ACG9CY_000877"
     CDS             complement(20510..21415)
                     /gene="xerC"
                     /locus_tag="ACG9CY_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS4937407.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21613..22989)
                     /gene="trmFO"
                     /locus_tag="ACG9CY_000878"
     CDS             complement(21613..22989)
                     /gene="trmFO"
                     /locus_tag="ACG9CY_000878"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS4937408.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22958..25036)
                     /gene="topA"
                     /locus_tag="ACG9CY_000879"
     CDS             complement(22958..25036)
                     /gene="topA"
                     /locus_tag="ACG9CY_000879"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS4937409.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25158..26015)
                     /gene="dprA"
                     /locus_tag="ACG9CY_000880"
     CDS             complement(25158..26015)
                     /gene="dprA"
                     /locus_tag="ACG9CY_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS4937410.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(26071..26838)
                     /locus_tag="ACG9CY_000881"
     CDS             complement(26071..26838)
                     /locus_tag="ACG9CY_000881"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS4937411.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26831..27691)
                     /gene="ylqF"
                     /locus_tag="ACG9CY_000882"
     CDS             complement(26831..27691)
                     /gene="ylqF"
                     /locus_tag="ACG9CY_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS4937412.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27951..28349
                     /locus_tag="ACG9CY_000883"
     CDS             27951..28349
                     /locus_tag="ACG9CY_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4937413.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28586..29128
                     /locus_tag="ACG9CY_000884"
     CDS             28586..29128
                     /locus_tag="ACG9CY_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4937414.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(29211..>29754)
                     /gene="lepB"
                     /locus_tag="ACG9CY_000885"
     CDS             complement(29211..>29754)
                     /gene="lepB"
                     /locus_tag="ACG9CY_000885"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4937415.1"
                     /translation="KQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSMENVL
                     KQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKVVKE
                     PYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTINKT
                     DILGKARFVYYPLDEIKWIQ"
BASE COUNT         7981 a         6259 c         5110 g        10404 t
ORIGIN      
        1 tgcaaacgat ctgcgaaaat agtttgtttt tttattttgc cattattttc ttacttattg
       61 ggagaaaaaa ttcgcttaaa ctcaaaaaaa tttgaggata cgatataaac tttcggatag
      121 agtataaaat aaggaaacgc cgcagcgttt cctttccaac tgtagccgaa tataaatttg
      181 tatatcactt atttaaaata gctattttct cccaaagagt ttccttgatc atcgatcaat
      241 ccttgtaatg agcgttctaa gataccatgg ctttttttcg attttttttc ttctgactgc
      301 tttgtttcct ctaacgtttt tgttcgattg aatgttcgta cagacggctc accatttctt
      361 ttttcttgat acggcgtcgg ttctgtatcg aacaacagat aagagtcatc cgtttttttc
      421 atcgattcga ttaaatgatc ctcagaaatt ttgctttctc tagggtctgg gataatcgat
      481 gcgggtacat atttaggaac aaaataagaa cgctcccgca ccttcgtgtc aacagatgcg
      541 tttcgtgtag tatgcttgga ctctgtcctt gtttcacttt gtctgacaga acgagaggaa
      601 tcaccaaaaa gtttttttcg tcctgtcgat gtgatttctg attggtaatg gccggtataa
      661 tccggtaatt ttcctttatg cttttccaaa ttttccttac gcggagctga cttcaatcca
      721 tttgttgcac gacgcttgcg acggattttc tcggtgttcg tgttcgtgaa atgaaattct
      781 ttcatctgtt caaacgggga ctgattctgt tcagtcaaaa gatcttcttg tccgtcaaat
      841 aatactcgct tcttgtcgtt ggttaatttt acgcctaact catcatcgta cgcaggaaaa
      901 cggaaatgtt ttcgtctgat ttctttcatt caaaccatcc ttctcaaaaa tggaactttt
      961 ctcaataata gcacagattc attgttttca gtagcaatgc gctgattatt ttaagaaaat
     1021 agcgatgaat ttcataaata tatatatagt aataagactt aattcgttgt ctctctgata
     1081 actagttcag gaaaaaaacg acgataacct tgactcgccc ctttttcttc tatttttttt
     1141 aacaacattt ctccacatac ttcacccata cgtataatat cttgcttgat cgttgtcaat
     1201 ttaggtgaac tgatttggtc taaaaatacc ccatcaaatc caatcacacc aaattcttcg
     1261 ggcaagtgtc ctccagcatt tataatccct cttactgctc ctagtgctag ccgatctgaa
     1321 ctacatacaa aacaagtttt atctgtaaaa tctgcccagt tttctgtcat atatctttct
     1381 acttctgtcg aatgatttgg aaaccgcaag attttactag gaagatcaac ttccttcatc
     1441 acagataaat aaccagtttc ccttgaaatt tcaaaaggct cgtccacttc gataccgata
     1501 taaataatcg tttgataatc tctttctaag gcatattgcg ttgcttttgc tgtaccatat
     1561 tcattatcgc tgtctacata atcatatcct tcagaatttt ccccaaacaa gacaactggt
     1621 ttttccaaac ggtcgatcca atcgaagtct ttttttctga cacctgtgat gatgtatcca
     1681 tcacaagaac cgacgtcgaa ggcatcattt gtaactaatt ggagagaata atgcgcttta
     1741 ccgatccgtt tagcgattcc catcagcaaa ttcatgtaat agggctcagt tgtgtcgatt
     1801 tcttccagta taaataattt gatgatctgc gaccgattcg tgacaagtgc ttttgcagca
     1861 atatttggac ggtaatccag ttcagccatt gctgagaaaa ctaactcttt caattcgtca
     1921 gtcacctgtt ccggatgatt gatgactcga gagaccgtca tttttgaaac attggcacgt
     1981 tttgcaacat ctgctaaagt gggcatgctc tcttcctttc taacatgttg tcattcttac
     2041 tatcaattat accaaatttt cctaacagag tttattatag cccaaaataa aaaacggaaa
     2101 atcctctatc gtcatgagtg attttccgtt tcaattattt atactttttt atcaaatttt
     2161 ttgctaaaaa agtggaagct tcttccatta gtggtatttg taccatatgt cgctgtgttt
     2221 ttgaggaacg aaaaacaatt tcttttccgt aagacttatg ccgatttttt tctacaaatc
     2281 gttccgtttg tatataaggg attttttcgt cagcttgtcc atgccaaaag aacagaggac
     2341 gtccagctaa cttttccggc tgcaatgaca aatcatagct cttaacccat gaaatcaaat
     2401 caaaatagtc ttttggtacg acataattat attccgatgc gcgccttacg atttcttttc
     2461 catattccaa aggtgcaggg gaacccatca aacacacgcc cgctactata tgaggatgtt
     2521 tagccaacaa ggcgcatgtt gtcattcccc ccatcgaaac cccgccaact gcaagctgat
     2581 catgtagaag acctcttttt tctaaatgct tgataagtgt gtcaaattca aacagatttc
     2641 cgtagatact atcccaaaat gtataggatg gaatagaaga aaccggctgt ttgcgctccc
     2701 catgattcat cgcatctggc aggatcacac gaatgccctg ctgcgcaatt tttcttcctt
     2761 gcgtcaaaac taactcctta cttgtctgcc atccgtgata atagatgaca acgggaatac
     2821 gttcctctct taattctttc agaacaactt ctaacactgg aatatgatgg acagaacgtt
     2881 tcaatataat tggattcaat tgttactcac ctattttcat taaatttcct gatcgccata
     2941 gcaaccatgc tccaacagtt gtctggaaca ggttcagtgt accaaataat ataaatgcag
     3001 gactgaatgg ctcgggaaat aagcacagag aacaaagtcc caccactcct aatgctagcg
     3061 aaaggagtgg ataaatagaa aaattcaaaa aactggcaac gctaccgaaa atattaccaa
     3121 taaacagtat caaaaagaaa aaaacgctta aaattaaacc atctgtcagc gacatctgaa
     3181 aaacaatgtt ctctacctca attggctgtt gaatcgaata gacacctaag tatatactga
     3241 gaaggagact tagcacgtgg ctggcacaaa acacacgatt gaccgttttc attttaatca
     3301 cctaacctat ctttataatt ctattataat cattttatca tttttttgta tgtataaagt
     3361 ttcaattttt cttcacttac gatttttttt gatattgttg ttatataaat gaaaggagtt
     3421 ttacaaatga ttgatccaaa aatgttgggg atgatcttcc tcattaattt tgcttatatc
     3481 actctgaaca cgttacgttt catgttgaca atgaaaggct atcgactgat tgctccactt
     3541 gtcagtatgg tcgagatcac gatttatatc gtcggcttat cccttgtttt agatcgttta
     3601 gataatccac tgaatttatt tgtctatgcg ttaggttacg cagtaggtat cagtgtcgga
     3661 attttgattg aagacaaatt ggcattagga tacatcatgg tcacaacgat tttgccagca
     3721 aatagcgcag aagataaaag tttgccgggt atactgagag aacacggtta tggtgtgaca
     3781 cagacttctg caatggggct ggaaggtgag cggctagttc tcgaaatact ttctcctaga
     3841 aaaagcgaac gtgaactgta tcgcctgatc aaagaaatcg aagagcgtgc ttttatcatt
     3901 tcttatgagc caaaatatat ttcaggtggt ttctggacta aaaaagtgag aaaacgacaa
     3961 ggaaaataac tcacaaactt aaaaactgtc taatatctaa attaaatttt aaatgttaga
     4021 cagtttttat ttattatttt gattaaaaaa catcgacata tactcatcaa tttgttatta
     4081 tactttatat acttatcaat ttaatttgga gatattatgg aaattcaacg tagaaccact
     4141 aattttttac atttaggctc tttttttgca actttgacga tcttattttt tctatgttat
     4201 tcatacctta agacctcaca tgcgccttct agtgcattag gctggttgct tttggatcat
     4261 tctgatatca cagaagccga agaaaatccg ttttttttga ttttgtcttc tctttgcggc
     4321 ttactgtttt ctgtttattt cggtgcctat ttttttcatc gacatcctgg tacgttaaac
     4381 gaagcttcaa gcgtattgat tgcatttaaa acgttttctt ttttagcaat tcttttttat
     4441 tcgtttaatc acaatacgct tttcttcttt atcatgaatg gtttagtcgt gattttatcc
     4501 atttttactt attttatttt ttcactcatt tttagccaat tgaaatgtcc gctcttcttt
     4561 cgattagttc ctgtcacctg ttttatttac agcttgtttt ccttttttgt ccttgcttta
     4621 atgccactga ccaacccttc gacgatccaa gtcgcaggaa atctgttgga tatgctcttc
     4681 atcgtcagtc taggtgtatt tatgtggcaa agaaaaaaca aacaaaataa aaagcatttt
     4741 gaagcctgac tattacgact tcataaatgc ttttttagtt ttaaacactt caatctaaaa
     4801 gaataaccca agattggata aattattaat gactcgccat tcgtttctta gtgattctgg
     4861 attatcaaat ggacttccta atctaatcag ctagttcttc ccataaaatg ccatacttga
     4921 ctgaatcata atattgatta tttaaaaatc taacttttct tatcactact tcctttgtca
     4981 ttccacattt ctcaccattt ttttgcattc ctttgtttcc agaccatgta gtgaatctaa
     5041 taggtggtag ataggcaaaa tactcaaata attcttttaa ccataaagga agagaaaggc
     5101 tcctcctgca agagtcagta taatttttgc ttactgctct cttaggtttt actcactaaa
     5161 actgttccat attctttgat ataagaaaat gcatgcggta taaatttctt gctcagctca
     5221 gaaaagattt gtcgtttaag aaaggccatt tcctccattg tcaatgaagg atccaatgtt
     5281 ggtaattcaa tctcaaaatt gtttttttcg acaataggta attctaatgt gaatgattct
     5341 tcttccttta tagtattcgt cacaagtaga gactgatgga aaatactagg ccggcgattt
     5401 ttatatttat tcttacgtat cctgtcaacg ataacttcta tttgatcaaa aaagtacgat
     5461 tcatttacgt ctccaataag ttcggctaat tctttgaatg ccttcgttac aggaagctga
     5521 ttcttctgta caaaaaagta attagccaaa gtaagcggga agtgttctat agaggaaagt
     5581 gtgataaaac ggtacttctc atttcctgca gttgtcctca gcaattctgt ctctcggctg
     5641 tttcttactc cataaaaata cgcttctttc cgttcatctt cgaaacaata cgcccaaata
     5701 acttctccca tcgctaattt ttgttcatca acagagagtc tcttcaattg ttcagctatg
     5761 gtttcagcag ccgaagtggc ttgttgcaag taatctttag gatcaaatat tggaaagttc
     5821 aaatgatatc ttcgttcttc cctcttgatt aatcctgcat cgatcaataa atcaagaaaa
     5881 tgggaaaatt tattttgcga aaattctttt ttcaattctc ttagaatcgt ttcttgttct
     5941 cgatgttcat atagataatc ggtcaatgaa gaaaaaagcg gatcatcggt cagtttttga
     6001 taaaagtttt ccttattttt tgaataatag ttcatatatg ttctcttttc tatccgtttt
     6061 attttactta aaaggctttt acgtaaaaag gaggaagaac aagagtcata attgactgtt
     6121 attctcctcc atttcttcgg cctttagtat gttctatgtt tctttttata tcaatcgctt
     6181 atccattcat tttgaatgct tcatactacc atttgattag ctaaatacag ccgtcaattg
     6241 aggtacgact tgttttttac gtgaaacgac acctttaagc aaagcacggt tgttggtcaa
     6301 agttgtttgg aaagcttctt ctactttttc ttttccttta ccagcaacaa gtaattctga
     6361 atcactgtct aagatgtttg tgacaactaa tacgaacaga tcgtagccat ttgctttcat
     6421 ttcatcttcc attgctgctt ctagctctgc ttgtcgatcc atcacttcat taaggtccac
     6481 tgtattgact tgagcgatac gaacgttgga actgcccatt gggaaacttt ttgcatccaa
     6541 atcaagaaga accgcagctg atttatcact tagatttgtt cctgctttca acatttcaag
     6601 tccataagtg tttagatcga cttcagcgat tgctgcaagt tcttttgcag ctgcttcatc
     6661 ttcaggcgta catgttggtg atttgaacaa caatgtatct gaaatgatag ctgagagcat
     6721 gattcctgcg atttctttag gaattgttac ttggttttct ttatatagtt ttaaaataat
     6781 cgtacttgta catccaactg gttctgcacg atagtacaat ggattagctg tttcaaagtt
     6841 tgcaatacga tggtggtcta ctacagctaa aatatttact tcgctgatat ctgaaatact
     6901 ttgttggaat tcattatgat ccacaagcat gacatctgca ttttctgctt tagctgactc
     6961 tactactcgt ggtgcagata gctgaaagtg gtctaatgcg tattgtgttt cttcattagg
     7021 tgtacctaga gcaactgctt ctgtttcttt acctaattca ttttgcaagt gtgaaaacgc
     7081 aatggctgcc ccgattgcat ctgtatctgg attttggtga ccaaaaacta aaatttttga
     7141 catgtcattc gctcctttgt gatttctcct atttaataat agcaaaaaaa cggaaagaag
     7201 caatacttat cttctttccg atgcgaccta tcgcgttaaa tattttgtat actcttctgt
     7261 atgaagcaat cgtttagcat tttcgacacg ttcttttgta ggtggttcga tcccttctaa
     7321 tggatatttc aaaccaagtg tttcccactt gtattttccc atcgtatgat aaggaagaat
     7381 ctctactctg tctacatttg acaaggtttg aataaagttg tctagtcgga tcaaatattc
     7441 atcataatca cttcgctcag gaactagtac atggcgaatc caaaccggtt tgttgatttt
     7501 tgacaaatat tgagccattt ctaaaatatt ttcattgtcc caatgcgtta gttttttatg
     7561 ctcttcatta tcgatgtgtt tgatgtcaaa taacagtaaa tctgtatact tcatcaattc
     7621 ttgaaaacgt gaaaagaaag gttcttcata cgtaaaaggt tttccgcatg tatccaatgt
     7681 agtatgcatc ttccgctctt ttgctttttt aaatagttcg atcaaaaaat cgatctgcaa
     7741 tagaggttct ccaccactaa cagtaatacc acctttatct ccccagtaag gtctgaagcg
     7801 ttctgcttta tctaaaagtt catctgctgt gtattctttc cctccgccaa tattccaagt
     7861 atctggattg tgacaaaatt ggcaacgcat gcggcatcct tgcataaaca cgacaaagcg
     7921 cagtccaggt ccgtctactg aaccgaaagt ttcgatagaa tgaacatagc cgattgtttt
     7981 ttcttccatt taagttcaca tcttttcttt gatttcattt taagtacaag ttagctgtcg
     8041 aagcgaagct ttctgaacaa aatttttcgc aattgccgct tcgttatgtg taaaatttac
     8101 agggacggaa catacaagtc ctgcccctgt aaatcgtttc tttcaaataa gcatactgat
     8161 tattcgattt tgatgcgatg gctattcaac gtcctttcga cgctaagtcg aaaaactttt
     8221 ttcacgaact ttttacattc tgtcgtgtga agttctcatc aagacatcta attgttgttc
     8281 tcttgtcaag tcacggaatt taactgcata accagatacc cggatcgtca agttcggata
     8341 tttcattgga ttttccattg catcacgcaa taaatcatta ctgaatacat tgacattcaa
     8401 atgataagct ccttttgaga agtatccatc taagacatta cgcaagtttt cgattcttgt
     8461 atcttgttct tttccaagac ctgaaggatt gattgtttgg gtatttgaaa ttccatccaa
     8521 tgcacagccg taattcattt tagctgttga gttcaggcta gctaataacc cactttgttc
     8581 cgcattgtaa cttggatttg ctcctggcgc aagtggtttt ccagcttcac ggccatcagg
     8641 agtatttcct gtatttttac cgtaaacaac atttgaagta atcgtcaata atgaagttgt
     8701 tggttttgag ttgcggtaag ttttatgacg tttgatttgt cccatgaagt attccaagat
     8761 ccatgaaact aattcatctg cacggtcatc atcatttccg tatttaggga agtcgccatc
     8821 cacttcaaaa tcaactgcta ttcctttttc attacggatt gcttttactt ttgcatattt
     8881 gatcgctgac aagctgtctg ccgcatgaga gatcccagca atacctgtag caaatgtgcg
     8941 ttgcaagtca gaatccatca aagccagctg agctgcttca taagaatatt tatcatgcat
     9001 ataatgaatg atgttcaacg tattgacata tagttctgct aaccaatcca tcatattcat
     9061 gaagttatgt ttcacttctt caaagtctag tatttctgat gtgattggac gatattctgg
     9121 tccaacttgg gcgccagtca cttcatcgac tccgccattg atggcatata atagtgtttt
     9181 agccaagtta gcacgagcgc caaagaattg catgtctttt ccgacaactg ttcctgatac
     9241 acagcaagca atcgcacaat catcagaacc ccattgttca cgaagctgat tatcattttc
     9301 gaattgaatc gaagaacttt cgatagcgat tctgctagca tatgttctga atccttctgg
     9361 aaggtgttca gaatacaaaa cagtcaagtt tggttctggt gaaggtccca tatttgttaa
     9421 tgtatgaagc agacggaaat cattctttgt aactaatgaa cgaccatcta atcccatacc
     9481 tgcaatggat aaagtagccc agatagggaa tcctgagaat aattggttat attcaggtgt
     9541 acgcgcgaat ttgaccatac gtaatttcat tactaagtgg tcgatcaatt cttgtgcttc
     9601 tttttctgtg attactccat tatccaaatc acgttggata tagatatcaa ggaatgctga
     9661 gatacgtccg attgacatcg cagcaccatt ttgtgatttg attgctgcta aatatccgaa
     9721 gtatagccat tggacagctt cttttgcatt tgcagctggt ttagaaatat catagccata
     9781 tttcgcagcc atttctttca tttgcgctaa tgcacgatat tgttcagtga tttcttcacg
     9841 taaacgaatc acttcttcac tcattgtatg ataaccagtc atatcatgat cttttttctt
     9901 ttgttccatc aaatagtcga ttccatacaa tgccacacga cgatagtcac cgatgatacg
     9961 tccgcgaccg taagcgtctg gaagccctgt aatgattttg tttttacgag cagcacgcat
    10021 ttcagcagta taagcgtcaa atacaccttg attatgtgtt ttgcgccagt cagtgaaaat
    10081 atgtgtcaat tcatcgtttg gtgtatagcc attgcttttt agcgcattgt tagccatatt
    10141 gattccgccg aacggcatga atgcttgttt caaaggtttg tctgtctgta gaccgacgat
    10201 tttttccaaa tctttatcca aatagcctgc atcgtgtgaa gtaatagttg atacgatttc
    10261 tgtgtccata tcatagacac cgttacgctc gaattggata tcatttaact tttgcaattc
    10321 atcccataat ttttctgttg cttctgttgg gccttccaaa aagctgtcat caccgcgata
    10381 ttcagtgtag ttattttgaa taaagtctct agtgtctacg ccttctttcc atttggtacc
    10441 tttaaatcca ttccattgct ccattgtatg gacctcctaa atttctgttt tatgtaacag
    10501 tttttacgtg attagtataa cacagcaccc tatctttgca agggttttct tgttttttat
    10561 ttaacaattt ttatgtttta tttacatata tttaaatcaa cgttgacata ccaacattta
    10621 agcattgtga acactttcat ctttttttgc aaacgtataa ggatttatta aaaatgaatg
    10681 aaaaagagac gccacctgta ttaatacaga tggcgtctct tcattaaaat actacaacag
    10741 ttacgactcg atgaaaatct caggagcatc gataacttcg atgacttctc cacctttttt
    10801 ctcatccatc gcaaatgatc cattcgatgt tcgatcacct atagggaaat cagttgggtc
    10861 aatcgttaac tgctgccctt tttgattcat caaggttatt tcttttgatc ttccttccga
    10921 catatagata atacggtgcg gatttttttt cagctcacgc atgaccgtca ctccccgctt
    10981 cgcacgtgaa gtttggctga tttcttgagc aagcatgcgt ttaacagctc ctcgctgagt
    11041 aacgattacg atcggcgtat caccttctgc taacacaagc agcccgttga taatcctatc
    11101 tccatctttc aaattcatag acttcactcc agctgccttt gctccaacaa cgggaacatc
    11161 tgttaaagga tagcgcaaac ccattccttg atgcgttact aagaaaacat ctaagcctgg
    11221 ctgatcttct ttcagataca cagcaatcag ttcatcttct tccgccttta gcttcataca
    11281 gctgagcgga cggctcttat aggtgcgcca tggttcgaac tctgtcattc gtgtttgctt
    11341 gatcaatcca ttcttgctga tgaatacaaa tgttttctct gcttgaattt tttgatatgg
    11401 gaaaacagct aaaatcgatt cgtctacaga cagattggag attgtttgag aaatgtgctc
    11461 accagcatct ttccatttca aatcgggtag ttcatggaca gggcggtaga tcacatttgc
    11521 tttatttgtg atcagaagaa catggtccaa tgtactcaat tcacctgagt atagcaagta
    11581 gtcgccttct cgcatcccga tttcttctgg tttggaagca ctataagaac gaaggcttgt
    11641 tcgcttcaca tagccttctc gcgtcacact gacaattacg tcttcttgtg ccactaagac
    11701 ttgcgtatca attttgattt cttcgatttc gtcttcgatt gtcgttagac gagcggaaga
    11761 ataatttttt ttcacttctc gcaattcttt tttcataaca gagaaaagct ctttgtcatt
    11821 tgaaaggatt ttattcaact ctgtgatttg cgcaatcaag ctttcggatt ccttttgaag
    11881 ctctgtgata tccgtgttcg tcaaacggta taattgcaat gtgacaattg cttctgcttg
    11941 ttcttctgta aattgaaaga cgtcgactaa attctttttg gcgtcttttt tgtctttact
    12001 ttcacgaatc gttgcgatta cttcatccag aatcgataaa gctttcatca atccttctac
    12061 gatatgctgt cttttttgag ctttggctaa atcgaattga ctacgttttg ttatgacttg
    12121 ctttcgatgc tcgatataac ttcttaaaat gtctttcagt ccaacttgat gcggagtcat
    12181 atggtcgata gcgaccatat tgaaattgta attgatctgt agctcagtgt ttttgaacaa
    12241 ataattcaaa attccttgag cattggcgtc ttttttcaat tcgacgacaa tttgcagacc
    12301 tgttctatca ctttcatctc gtacttcagc tattccatcg attttcttat tcaaacggat
    12361 ctcatccatt tttttcacca aggtcgcttt atttacttca tatgggattt ctgtgatcac
    12421 gatttgctgt ttattaccct tcattgcctc tatctttgtt ttcgaacgaa gaataacttt
    12481 tcctcgtcct gtttcataag cttttttgat ttcatctttt ccttgcaaga tccctcctgt
    12541 tggaaagtca ggtccaggaa tatattccat caatttttca aggctcgctt gtggatgatc
    12601 gatcatatag attgtgccat cgatcacttc agctaagtta tgagtcggga tttctgttgc
    12661 atatccagca gatatacctg tcgatccatt gacgagaagg tttgggtatc gagctggaag
    12721 gactgttggt tctttttctg tatcatcaaa attccacacg agatcgactg tttctttctc
    12781 gatatctgct agcatctctc cacttaactt ggataatctg gcttcagtat aacgcatcgc
    12841 agcaggtgga tcaccgtcca tactcccgtt attcccgtgc atttcgatca gtacttcccg
    12901 caacttccag tcttggctca tgcggaccat cgcttcataa atactgatgt caccatgagg
    12961 atgataattc cccatgatat ttccgacaga ttttgctgat ttacggaatc ctttgtcata
    13021 cgtattgcca tctttgttca tggcaaataa tatccggcgt tgcactggct tcaatccatc
    13081 acggatatca ggcagtgcac gttcttggat aatatatttg gaataacgtc cgaagcggtc
    13141 acccatcact tcttctaaag ttaattcttg tacttcttgt cgattttcca tcaggtgacc
    13201 tcctattcta cttcaaataa ctgattgttt tcatttttat ctgctcgttc ttcatctaat
    13261 aaatcattag aaactgaagg agagatttca gtatcctctt ttttatctaa gatactgcca
    13321 tcttcttcga gcgtaaattg cacgtgattt tcgatccatt ttcttcgagg ttctactttg
    13381 tcacccatca gtgtggttac tcgacgttca gcttgcgcag catcatcgat acggacgcgg
    13441 atcaacgtac gggaagtcgg atccatcgtt gtttcccaca attgttccgc attcatctct
    13501 ccaagtcctt tatatcgctg cagcatatat cctttgccga cttttttaat cattgctgcc
    13561 aattcgtcat ctgtccacgc atattcaatg acttgcttct ttccttgtcc tttggatact
    13621 ttatataaag gcggtaacgc gatatatact ttcccagctt caatcagcgg tttcatataa
    13681 cgataaaaga aagtaagcag taatacttga atatgggcgc cgtctgtatc cgcatcggtc
    13741 atgatgatta ctttgtcgta attacagtct tcgatagaaa actcaggacc gactcctgca
    13801 ccaatcgtat agatcatcgt attgatttct tcatttttca agatatcttg cattttagct
    13861 ttttcggtat tgatgacttt tcctcgtaaa ggtagaattg cttggaactt tctgtctctt
    13921 ccttgtttag cagatccgcc agcagaatcc ccttcgacta aatacaattc atttttctta
    13981 gggtttcggg attgtgcggg tgtcaatttt cctgaaagaa gagattcgcc ttttttacgc
    14041 ttcttcccat ttcggctttc ttctctggct ttacgtgctg cttctctcgc ttctctggct
    14101 ttgattgctt tacggaccaa catctggctc atttcgctat tttcctgcaa ataaaagccc
    14161 atttgttcac tgatgacgtt atctactacg gtacgagcaa ctggcgtacc aagtttttct
    14221 tttgtttgcc cttcaaattg cagaagattt tcaggtacac ggatagaaag tacagcagct
    14281 aacccttctc tgaaatcact accttccaga ttcttgtccc gttctttcag caaaccaact
    14341 tttctcgcat attcattata agatttcgtc atcgccgctt tcataccagc ttcatgggtt
    14401 cccccatctt ttgtccgaac gttattgaca aaagataaaa cattttctga ataaccgtca
    14461 ttgtattgat aagcgacctc tacttcgata ccttcttttt ctcctgaaaa ataaacaact
    14521 ggtgtcaatg tatctttttc ttcattcaga taatcaacaa attctttgat tccttcttcg
    14581 taatgaaaaa tttccttgac tggttcttct ccacgaagat cagaaagaga gattttaacg
    14641 ccttttagaa gaaaggcaga ttctcttaac cgttcggcta aaatttcata tgaaaatttc
    14701 gttgtactga atattgtatc atctggtaaa aaatggacag tcgtcccgtt tgcttttttt
    14761 gtttttccta tctttttcaa agtaccgtca ggtttaccgc cgtttttaaa tttctgctga
    14821 tactctacgc catctctaac gatcgttaca attagccatt tcgataaggc atttaccaca
    14881 ctggctccta caccgtgcag tccaccagaa gttttgtacc ctccttgacc aaactttccg
    14941 ccagcatgca gcacagtaaa aatcacttca actgttggta ttccggaagc atgcattcct
    15001 acaggcatcc cacgtccgct atctgcaaca gttatactat tgtcttcatg gatggtcaca
    15061 tcaatctcac tgccataacc tgataatgct tcgtcaactg cattatcgac gatttcatag
    15121 actaaatgat gcaagcctcg gctgtcagtg gatccaatgt acatccctgg tctttttcta
    15181 actgcctcta atccttccaa tacttggatg gacgagtcat tgtattcgtt gtttactttt
    15241 tttgccaagg caaacgctcc ttcaataata ctttgcggca aaaccgcacc acgattatca
    15301 tactagaaaa ggtcgtgaaa aaaaagaggg tagacgagaa atcaatttta attatctcct
    15361 aaccgctttt tcgacattcc cctctatcga tcatctcttc tttgtggtca tttttatttt
    15421 tggttttctt tcaacttacc aagttcaatc ttaatacaac gatccattac gatatctgta
    15481 cgtcctgctt ttcttagcat ctcagctgct tctttgtttt caatccctaa ttgtgcccag
    15541 aacactttag catcagtttt caaaaaatca cctgctattt caggtaaaaa ttcactccgt
    15601 ctaaatacat ctacgatatc gattggatca tcgatttctg tcaaattcgc atagactttc
    15661 tcgcctagaa tttcttcacc agccaataat ggatttactg gtatgatttt atatccattg
    15721 tcttgcaata cctgtgcaac catgtaactt gttcgttctg gtttattgct caagcctacg
    15781 acggcaatat tttttgcttc ttccaaatag ttgaaaatcg tttcttgtgt tggatttttt
    15841 acagacattt ctgccacccc ttgcttttct ttttattcca tctattatac ttgtatcttt
    15901 cattttttca attgttcctg ttacctattc atgctaaaat aaaggatagt tttcaacttg
    15961 aaagagggta aaaaatgaaa ttgatcttat tacttatttt agcatatctt ttaggttcta
    16021 ttccttccgg tgtctggatt ggcaaattgt ttttcaaaaa agatatccga caacacggga
    16081 gcggtaatac tggaacgacc aatacatttc gtgtattagg caaaacagcc gggattgttg
    16141 tcctgcttat ggatatttta aaaggtacat tggctacttg tttgccgatg atcttccacc
    16201 tttctgggat taaccctctt tggtttgggg tatgcgccat tcttggccat acatttccga
    16261 tttttgctgg gttcaaagga ggaaaagctg ttgcgacaag tgcaggaatg cttttaggat
    16321 ttaatcctgt attttttgtt tattcttgta tcatctttat cgttagttta ttttgtacaa
    16381 gtatggtgag tttgagcagt atgatcagtg cagtattgat cactttatct acgatcgtcc
    16441 ttccttatgt agctcctgtg attttggcta agcccaattg gttactgacg atcattgcct
    16501 ttttagtttc ttcctttatc ttttatcggc ataaagacaa tatcaagcgt atcagaaacc
    16561 atactgaaag tcgaatcccg tttggtttga atagcagcaa gaaaaaatcc tgatactttt
    16621 tcattccaaa gagataaaac gggagtgtga caaatcgatg tcacactccc ttattactta
    16681 caggatagct tttttacaca ccttggtaaa cggtgttccc tgctaacttc tcggtcacaa
    16741 cctcttactt cactgtgatg gaatattttg tcttgaagat ttcatgggca cctagttgat
    16801 tgatccctac tttttccatc aagtttccac tactgttcac cgtgtcagcg attccgcacc
    16861 acggttcgat acaaacgaaa ggcgcttctt ttggataagg agaccagatc cctacataag
    16921 gcatatcttt atagcttaaa gttacgctat gggaagattt ttcacttcga atggcgtacg
    16981 catttagacc ccttgtttca aaaaccaagg catcttgctc aaaaagttca cgattcaaag
    17041 ccagattcgt atttgtctga cctaacgtct tttgatccat atcgatgaat gggcctttca
    17101 atggtaggcg taatcttgat ttcaatggag aaaatgccag atagaaatct tcaaatgaca
    17161 atccttcttc aagaggtaca ttgaaagcag gatgtccacc gatagaaaag aacatttctt
    17221 tttcaccggt atttgccact tgataatgaa ccgtgatgcc gtctcctcct aattcatagc
    17281 tgatcactaa ttcaaaatca aatggatact tttctaatgt ctccggcgta ctccttaaga
    17341 tgaaactagc catttcttgt ccatgttcaa tgacttcaaa atccatatcc cgagcaaatc
    17401 cgtgttgccc catttgatat gttttccctt gataaacata ctgatcatct tttaaacgtc
    17461 caacaaaagg gaataaaacc ggtgcatgtc tgccccaata cgctggatca ccttgccaga
    17521 tatactctaa accatgttct tttgatttca gactgatcaa ttccgcaccg tgttcagcga
    17581 tttcggcgat cagctggtca ttttgaataa taacagtcat tgccattcct ccaacttcga
    17641 cttacttttt aaaatgggaa ggctggaaca aacatttcta tctgccccag tccccttact
    17701 tctaataact gtgttttgct aactcgtctt taaaacggga attcaatact tttaaataag
    17761 ttcccttcat gcctaacgaa cgagattcaa tgattccagc tgattccaat tttcgtaaag
    17821 cattgacgat cacagaacga gtgatgccga tactatcggc aatattagaa gcagtcagac
    17881 gaccttcatc tccatccaat gcttcaaaga tagcttgtac agccttcaat tcgctataag
    17941 ataatgtgtt gattgccatt tggacagccg ttgcactccg tacatctgct tcgatgcttc
    18001 gagacttctg ataaaggatc tgcataccga caaccgtagc tccatattct gctaagacta
    18061 gatcgtcatc tgcaaacgat tgttccatac gagataagat aatcgtaccc aaacgttcgc
    18121 cagctccaaa gatcggaacg acagtcgtca gaccaaatgg atatttctct cttgactcta
    18181 ccggaaatgc agtaatatca ctagtaattg ggatattagc ttcagttttc atcaactgtg
    18241 ccactgtatc agtataagat tcaggaaatt gtttctcaac aaacattgtt ttgattcgtt
    18301 cattgttcac atcgtgacgt tcattataac ctagtaatac tccgtcactt cctatgatat
    18361 aggtgttgct gtcaagaata ccacctagtg tgacagccat tttatcgtaa ggcaaatctg
    18421 cttgcatgtc aaaagtattc ttttgctgca aaagtgtgtt tatcttgcgg gttttcgtta
    18481 gcaagtctgt cattgtcgtc ctccgatttt ttataaaata tagcgactta aatcttcatt
    18541 tgccgcaatt gagccaagtt tgtcatccac atatgcttct gtgatcgtta tttctcccat
    18601 ttgcatatct ggtgcttcaa ataatagatc ttctagtagt ttttccaaaa tggtatgcag
    18661 tctgcgtgcg ccgatattat ctgtgtcacg gttgacatcg aaagcgattt gagcgatccg
    18721 ttcaattgct tcttttgtaa acgtaactga cacattttct gtaccgatca acgcgacgta
    18781 ttgttttact aaagcattat ttggttcggt cagaatcttc acaaaatctt cagctgtcaa
    18841 atcatctaat tctacccgta tcgggaagcg tccttgaagt tctggaatta gatcacttgg
    18901 tttagacaaa tggaaagctc ctgaagcaat gaataggacg tgatctgttt ggatgatccc
    18961 gtattttgtg tttacttgtg aaccttcgac aattggcaat atgtctcttt ggacgccttc
    19021 tctggatact tctccagatt gctgtgattt agaagtgatc ttatcgattt catcgataaa
    19081 aataattcca ctttcttccg ctaatttgat tgcctcacta tggatatcag catccttgac
    19141 taattttgct gattcttcct tgatcaataa ctctaaagct tctttgactg ttaccgtccg
    19201 ttccactttt ttcttcggtg tcaaagcacc tagagtttca tttaggtcga tccccatttg
    19261 ctccaatcca ttgttcatca ttggtgcagg tttcttttgg tcttcaactt caatggttac
    19321 ttcacgacta tccagtaaac ccttttgtag ttgttccaag actgtacggc gattgattcg
    19381 aatatcttct gtcacttctt cttgcggttc agcggtattt tgctgactag cattaaaaag
    19441 attcatcatt tgttcaaatt gattagtgga ttgtttctgt tcttttttga taccaggtac
    19501 caagattttt accaaacgtt tttctgcatt ttttttagca cttgtgtata cacggctgta
    19561 ctgctctttt tctacgattt ggatcgcatt ttctactaaa tcccgaacca ttgattctac
    19621 atctcttccg acataaccga cttcagtaaa ttttgttgct tcgactttga caaatggtgc
    19681 attgacgatt tttgccaaac gtcgtgcgat ttctgtttta ccgacccctg taggtccgat
    19741 catcagcata ttttttggtg taatatcttg ttgcattttt tcatctaact gcaaacggcg
    19801 atagcggtta cgcaaagcaa cggcaacaga tttttttgct gcgtgctgtc ctacgatata
    19861 ttcatctaat tcacttacga tttgtcttgg tgtttgtttt aattcataca tatctactca
    19921 cctcttacag ttcttctacg ataatattat gattagtaaa gatacagata tctgcggcaa
    19981 tatttaacgc attttctgca atttctttcg cactcatgtc gttttgagca tagtttttca
    20041 ttgctcgggc tgcagccaac gcaaaattcc ctcctgatcc gatagctaag ataccatcat
    20101 ctggtgtgat aacttctcct gtacctgaaa caagaagcat ttctttatca ttcattacga
    20161 tcagcatcgc ttcaagtttt tgcatagatt gctgtgtccg ccattcttga gctaattcga
    20221 cagctgcgcg ttgtagattc ccgttatatt cgtttagttt gccttcaaat ttttcttcta
    20281 aagtaaacgc atctgcaaca cttccggcga aacctacaac aacttctcca ttgtagatac
    20341 gtcgtacttt ctttgcagtg cctttcatga caacagattc acccattgtt acttggccat
    20401 ctccagccat agcaaatttc ccgtcttttt cgacggcaca gatagtggtt gaatgaaatg
    20461 gtgattcgac catgtttgtt tcctcctaaa tttgttcttt tccttatttt tacgctcttg
    20521 gatggaaaga acgatagttt ttttgtaaac tttcttttgt tacatgggca tagatctggg
    20581 tagtcgataa attggcatgt cctagcaatt cttggactgt ccgcaaatct gccccattat
    20641 ttaataaatg agtagcaaat gtatggcgca gcatgtgagg atggatatca ctatccaaac
    20701 tgcttttttt gatcatttga ttcaagacat attcaattcc agtaggcgtg attggttttc
    20761 catgatgatt cacaaaaacg atatcatgct cttcgtgata ttttgtcatc aatacctggc
    20821 ggccttccct gaaatattct tgcagagcat cttgagcaaa agaacctaga ggagcatatc
    20881 tctctttgtt ccctttacca tggatcaaca tgacctctga atcaaaatca atgtctgata
    20941 ccgacaaact gctacactca cttaaacgga tgcctgaccc gtaaaggact tccagtaaag
    21001 cgcggttcct caaatccaaa ggactatctc ctgctacgct gtcaaaaagt gcttgcattt
    21061 cattttcata aaaaaaacgt ggcagtctca aatttttctt tttgagatga acgtaagaaa
    21121 atggattttc ttttatcact tcttctttga gaagatactg ataaaacgaa cgtaggctgg
    21181 ctatcttgcg actgatagaa tttctgctat agctaggacg atgatcagct aatgcactca
    21241 aatagacacg aaccgtaaaa tattccgcct tcagcagatc agcgtctcca gattcctcca
    21301 aaaaatgttt gaaatggaga atgtcttctt catatgcttc tttcgttttt tctgaatatc
    21361 ctctttcgac aatcaaataa cgcaaaaatt tttgttccca cgtggcttct gtcattttct
    21421 caccttcctt ttgtcccaga gaataattta gcacagcgtt tataaaaaga caaataaatt
    21481 gtcagaattt aaccaatatt tctaagtttg ttcacaattg gcgcaattcc tcacattttg
    21541 tttgagaaat tcttaagaaa aacgcaagaa aaagagcgag actatccgtc tcaccctctt
    21601 catggagttt ccttactcat ttgggattgc agttttttct aattctgttt ttattgcctc
    21661 attagctttt aaagctcgtt gtgcaattgc ttcatatctt gcttttttgt ctcgaatgcg
    21721 ctctggcagt tcaggaaaca gaccatagtt ggcattcatc ggttggaaat gttttccttc
    21781 cgcatgagta atgtagtaag ccatacttcc aatagctgtt tcttgtggaa actcgactgg
    21841 ttccatttct tttgctaagc gtgcggcgtt gattccagca agtagtccgc tcgctgcact
    21901 ttcaacatag ccttctacac cagtcatctg acctgcaaag aacagatctt cgcgtttcgt
    21961 tgattggtaa gtcggtttca gcaattctgg cgaattcata aagctgtttc ggtgcatcac
    22021 accatagcga acgaattcag cattttctaa cccaggaatc atccggaaaa cacgtttttg
    22081 ctcgccccat ttcaagtgag tctgaaagcc cacgatgttg tataaagaag ctgcagcatt
    22141 gtcttgacgc agctggataa cagcataagg acgtttgcca gttttaggat cttccaatcc
    22201 aactggtttc ataggtccga aaagcatcgt tttgaatcct cgctgtgcca ttacttcaat
    22261 tggcatacat ccttcaaaat atttttcttt ctcaaaagat ttaagttctg ccacttcggc
    22321 attgattagt gcctcatgga aagctttgaa ttcctcttct gtcatcggac agttcaagta
    22381 agctgcttct cctttgtcat agcgggactt caaatatact ttatccatat cgatcgtagc
    22441 tttgtctacg attggtgcag ctgcatcata gaaataaaaa ccatctgaac cattgaatgc
    22501 tttgatgctt tctgctaatg gatgagaagt caaaggtcct gttgctacta cggtgattcc
    22561 ttccggtatt tcagtaattt cttcgtttat cactgttact aatggatgct ctttgacttt
    22621 tcgggtaatt gtttcagaaa aagaatcgcg gtcaacagct aaggcaccac cagctggcac
    22681 tgctgtttca tctgccgaag tgattactac cgaattcaat cggcgcattt cttctttcaa
    22741 taaccctact gcattagcca aactgtttcc tcttaatgaa ttggaacata ctagttcagc
    22801 aaaattactt gtgtgatgcg cctcagtcga tttgactgga cgcatttcat ataaacggac
    22861 agggactcct gcttgtgcga tctgccaagc agcttcactt ccggcaagcc ctgctccaac
    22921 aacatttacc attttcaatg aaagaacctc ttttctatta tttttggatg tcttcttcgt
    22981 aatctccatt tacacagact acttgtttac cgcctttgac tttcttctcg accagatatc
    23041 cgtcacattt tggacaattt ctgcccactg gtttatccca agaagtgaat tcacattctg
    23101 ggaaacggga acaaccatag aatagtctgt ttttcttaga ttttcgttca atgacttgtc
    23161 cttcatgaca tactgggcaa gtcacaccga tttcttttac gattggtttc gtattccgac
    23221 aatcaggaaa attactgcaa gcataaaact tcccatattt ccctaatttt atcaccatcg
    23281 gatgtccaca gacatcacaa tcgaatcctg ctggttcatc tttgatttga attttttcga
    23341 ttttttcttc cgcattcgtc agttcttttt caaacggttt atagaaacga tcaaccactt
    23401 tgacccacgc ttccgtgcct tcttcaattt tatccaaatc tccttccatt tctgctgtga
    23461 aatggatatc gacgatttga gggaagaatt cacagattaa tgaattgaca atttctccta
    23521 gttcagtcgg ctcaaatctt ttttgtgtca attttacata gtagcgtctt tggatcgtat
    23581 caagtgttgg tgcataagtg gaaggtcgcc ctaccccgtt ttcttctagt gcacgaatca
    23641 acgtagcctc actaaaacgt gctggtggtt gagtaaaatg ttgttttggt tcaatatcaa
    23701 cagattggac tacatcgcct tcttcaagtt ctggaagaat attttctttc tcttctttgc
    23761 cgtcatctct accttccaca tacacttgca tgaatccttt gaatttgatt tttgaaccgt
    23821 tcgcaatgaa gatgacacca ttttgttcta atgtcacttt cattgtatct aaaatagctg
    23881 gtgtcatctg actagccaca aaacgtgacc agatcaatgt ataaagtttc aattgatctt
    23941 tatctaaata ttgtttgagt tcatctggtg tacgaagcac actcgttgga cgcacagctt
    24001 catgggcatc ttgtgcgcct tgagcatttt tgatctttcg gtgatcatgt ctagaaaatt
    24061 cttttccata cgtatcttca ataaatgctg cggcctctgc ttttgctgta tcggcaatcc
    24121 gggtagaatc agtacgcatg tatgtaatca agccgactgt tccttgttta cccaaagtga
    24181 ttccttcata taattgctgt gctaccatca tggtttttct tgttcgaaag tttaacttac
    24241 gggcagcttc ttgttgcagg ctacttgtag tgaaaggatt cgcagggttt cttttgcgct
    24301 ctttcttttc tactttttta acttcgaagt ctgttccatc aattctagca gtgacttctt
    24361 ttacactttg tgcatctggt aatttctttt tcttaccgtc gattccccag aaatttgctt
    24421 tgaatttctt gcgagctttt ttaaaattac cgtcgatgct ccaatattct tctggaacaa
    24481 attctcggat ctctttttca cggtcaataa taatttttaa agcgacagat tggacacgtc
    24541 ctgcacttaa ccctttcttg accttacgcc agagaatagg actgatcgaa taaccaacga
    24601 ttctgtctaa ggtacgacga gcttgctgtg catctactaa atctacatcg atcgttcttg
    24661 gttccttaaa agctgctttg actgcgtctt ttgtgatttc attgaagacc acccgatttt
    24721 tttctttcag atccaaccca agtaggtacg ctaaatgcca agcaatcgct tctccttctc
    24781 tatccgggtc acttgccaag taaacttttt gagctttttt tgctgcggct ttcaggcttt
    24841 tgatgacatc gccttttccg cgtatagaaa tataatgggg ttcataattg ttttcaaaat
    24901 cgatacccat tttacttttc ggtaaatctc ttatatggcc aacactggcc attactttat
    24961 agtttcgtcc aagatatttc tcaattgtct tggcttttgc aggtgattct acaatcacta
    25021 aatatttata cgccattact gaatggactc ctttccattg tcgtgttcct actattaaaa
    25081 gaggattcat acaaatcgta gctaatcata tccattcaga aaacgtttgt caagaaaagc
    25141 aaatgaaaag tatggtgtta ttctttaaat tctgccaaga tatcttcacc cgaaaccgtg
    25201 caaactgcac cgtcttggat caaatgatga catccggtag aatgttctgt taaaatattt
    25261 cctggaacag aaaaaactgt tcttccaaga tccaatgctt gctgtgctgt tatcagagag
    25321 ccgctttttt tcttggcttc aaagacacat gttcctttag acaaagctgc gataatccga
    25381 tttctcattg gaaaacaatg ctttttcggg ccggattgag gaggatattc actcaaaaga
    25441 agatgatcat ttgcaatcac ctcttgcaag tcacgatttt cagcaggata gacttgctct
    25501 atccctgttc caatgactgc gattgtccgt ccaccatgag agattgctgc ttggtgaccg
    25561 tatgtatcta tccctttcgc gcagccactg acgatcacat atccagcttc tatcactggt
    25621 tttagtaaat aataaactgt ttttttggct aaagcagaag tgtgtcttcc accaacaacg
    25681 cttaacatgt tacaagaaag taaagataga tcaccattat aaaacagtac aattggcgga
    25741 taggtcaagt gaagcagctg agggggatat ttgggatcaa agcaagtgat aaacgactgt
    25801 ttcgattgga ttttgcccca gttagcgtga atttcctgcc aacttttgcg aaacagctcc
    25861 cggtgtttgg ttattttacc tatcttaatg atgtcttcaa atccgatttc tttataagga
    25921 tactgcatca tccagttagc aagtgcccat tttccttgac tactgattcc ttttgtataa
    25981 gcaagtttca ataaattttc ttctatttga tacatcataa aaccgccttt cgtctctctt
    26041 tctaataaga tacgtaaaag cggctgtttt ttattgtaaa tattttttta caggtgcaaa
    26101 acttttacga tggattggcg taattccttg tcgttcaagt ccttctaagt gttcctttgt
    26161 tccataacct gcattatgcg agaaaccata tccaggataa agcacgtcat attctttcat
    26221 caattggtct cgatatactt tagcaatgat acttgcagca gcaatcgaca cagatcgtgc
    26281 atcccctttg attatttttt cctgctgaat aggcaaatcc aaggtcatcg catcgatcaa
    26341 caaatagctt ggtacaatgg acaatttttc tactgcttgg atcatcgcta atttacttgc
    26401 ttcataaata ttcacatcat cgattgtctg ctgatcaacg atcccgatcc cgattgccag
    26461 ggctttttct tgaacttgtt gataaagaag ttctcgcttt ttctctgata attgtttgga
    26521 gtcattcagc cctaatatga tctcattttt cggcaagata actgcagctg ccacgacagg
    26581 tccagctaat ggtcctcttc ctacttcatc aatgccggca atccattggt gaccttgtgc
    26641 gtacccttgc ttttcaaagc aaaacatttc ttcatatttt tccttaatta atgcttcctt
    26701 tttcaatcgt cgttcaaaag aagccagtgc ttgctgcacg cctttgcgtg gatcatttct
    26761 taatgattct agtgtaggat catctattct ctgtatgctt ttcaatcgtt ctttgatact
    26821 ttggatggat tcagtcatca cttttttgct ccatttcttc gcaacgatcc agtgtgtatc
    26881 gtccaagttt tccttgacga atatcatgca cgactaattc gctgccacga tcatagtctt
    26941 ctttataccc acgttttgct gtaatatcca tcagtaattc cggtgccaat ttttcttctt
    27001 gctcgattga taaaccataa cgatcgctga ttcttcctga ataatatcgg gcaaaaaagt
    27061 ctaatccgta aatcgctaaa tcatctaaat ggacaagctg gtctttgatc gcgcctgtca
    27121 gcgctagttt tttgccgatt tcttcatctt caaatttggg ccataaaatc cctggtgtgt
    27181 ctaacaattc caactcatta cctaaacgaa gccactgctg accttttgtt acgccaggct
    27241 tatttcctgt ctgtgcaatt ttcttgccta ctagtcgatt catcaacgta gatttcccaa
    27301 catttggaat acctaagacc attgcccgaa tagcgcgagg cttgatcccc cgagcagcat
    27361 ctcttgcccg tttttctttc aatgcttctt tagccgcagg tactagacct ttaattcctt
    27421 tactttcttg tgccgtgata gctaatgcaa gatggccttg tttacgaaaa tattcttgcc
    27481 acagctttgt ctgttgtgga tctgccagat ccgctttatt caataaaaca aggcgaggtt
    27541 tttgctgaat gatctgatct agcattggat tacgagaaga taaaggtaat cgtgcgtcta
    27601 ccaattcaaa tacgatgtcc acatatttca ttttttcaga aacttctcgt cgggcttttg
    27661 ccatatgtcc cggaaaccat tggatggtca ttgttctctc cattcctttc ataccggaat
    27721 attccccggc tttacttgtt tttttctttt tcaatataaa ttcacttttt tattataaag
    27781 aaattccatc tattagttaa gtgaaagtga accagtcctc attctatcac atcttcactt
    27841 ttttgatgat gcaaattttt attttggttt ctatatatga atctaaattt ttgacagcgt
    27901 ttcctaaata agagatgata gacgaacaaa acagaaagga gacgaaacaa atgaatagta
    27961 tggtttttgt taattttcct gttgctgatg tcaatcgttc cgttgctttt tatgaaaagc
    28021 tgggttttaa aaaaaatgaa gaattttcta ctgaagaagc tggttcaatg gtatgggatg
    28081 acactttttg gataatgctt ctctctcatg atttttataa aaaatttata aaagataaag
    28141 aaatagctga taataaaaaa acaagtggtg ctttgatttc gtttagtgtt gaaaatgcgg
    28201 atgaagtaaa aaaatttgct caaattgcaa aagaaaatgg tggtactttc cacgaagtag
    28261 atatgggaat gcctgaagaa gaaatgtttt cattagaagt ccaagatcta gacggaaata
    28321 cattagaacc cgtatggatg aagatgtaag cagataacta atctgacgtt ttttataaca
    28381 aaaaaacaaa ggcggatgat acgataagta ttctccgcct ctattttttt tattcgtaca
    28441 atctcttttt tacagtttat aaaagcagtc ttctcaccct ttttttaatt gttagatgcc
    28501 agtgtatgta aatcaccaat gtgtcatgac acattggtga ttttatggta tccttttatt
    28561 attaactgac aaaggagaga atattttgaa cactttgatt gcaaaagacg tttttagatt
    28621 gcccgaaatg aatgcaagat tgttgtcgac ctctgctctt ttattagccc ttgatgtcat
    28681 cttatacaag atagcgattg gtcctgctta ttttcaaatc acattcggat ttttaagtat
    28741 ggcgcttatg ggttatctat atggaccgat ctgggctggt ttgcttgaaa tgttgtcgaa
    28801 cactttaaat ttcacgatat ttggttcagg aacgtttcaa gctgcttttc ttatcacggc
    28861 tttccttggt ggatttatta atggcttatt tttatatcat aaaaagataa acctgccttc
    28921 cgtcatcgct gcccaattat tgattatgat cccagtcagc ctttttatca attcttggct
    28981 gatgtctgtt ttgtttggtg cagatttcaa agtaattttt ctaggtagat tcatcaggaa
    29041 tatgatccaa atccctattc aaataatggt tgtttattca tttatcaaaa ctttagaggc
    29101 tagaaaagtg tttaaacgag ctgtttgatt ttacgtacta aggtagtaaa ctgattttgg
    29161 attaaacaat aaagcgtgca gtctcaacag actgcacgct ttattgtttc tcactggatc
    29221 cattttattt catcaagtgg ataatagaca aatcttgctt tacctagaat atcagtttta
    29281 ttgatcgtgc caaacgaacg actatctttt gaaactcttc gattatctcc aagtacaaaa
    29341 taattatctt ccggaagctt ggattgtccc gttagctctt gtagtgtaaa gtccgtcgta
    29401 taggatgcat ttgcatgatc tgatttcagg tttttagtta gataaggttc cttaactact
    29461 ttcccattga tcttcaattg atcattttgg tagctgacat tttctcctgg aagaccgatt
    29521 actcgtttga tataaatcgt gccatctgct aattgaaaaa caacaatatc aaatcgtctg
    29581 atctccgaaa atttttccat cacaaccata tcaccttgct tcaacacgtt ttccattgaa
    29641 ttgccttcca ctggtaccgg gatgaataaa aatccccgca acatgaacgc aaggaataca
    29701 gaaacaagta agtatttgaa tacaagccag aagcgattaa tatagcgttg tttt
//
LOCUS       DAZULG010000021        28255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000021 DAZULG010000000
VERSION     DAZULG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.021"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..721)
                     /locus_tag="ACG9CY_000886"
     CDS             complement(<1..721)
                     /locus_tag="ACG9CY_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4937416.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIAN"
     gene            complement(797..943)
                     /locus_tag="ACG9CY_000887"
     CDS             complement(797..943)
                     /locus_tag="ACG9CY_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFS4937417.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(1047..1358)
                     /locus_tag="ACG9CY_000888"
     CDS             complement(1047..1358)
                     /locus_tag="ACG9CY_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4937418.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(1360..1557)
                     /locus_tag="ACG9CY_000889"
     CDS             complement(1360..1557)
                     /locus_tag="ACG9CY_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4937419.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1951..3678)
                     /locus_tag="ACG9CY_000890"
     CDS             complement(1951..3678)
                     /locus_tag="ACG9CY_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS4937420.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(3671..4864)
                     /locus_tag="ACG9CY_000891"
     CDS             complement(3671..4864)
                     /locus_tag="ACG9CY_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4937421.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(5051..5695)
                     /locus_tag="ACG9CY_000892"
     CDS             complement(5051..5695)
                     /locus_tag="ACG9CY_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4937422.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(5789..5923)
                     /locus_tag="ACG9CY_000893"
     CDS             complement(5789..5923)
                     /locus_tag="ACG9CY_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS4937423.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5925..8057)
                     /gene="feoB"
                     /locus_tag="ACG9CY_000894"
     CDS             complement(5925..8057)
                     /gene="feoB"
                     /locus_tag="ACG9CY_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS4937424.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(8054..8527)
                     /locus_tag="ACG9CY_000895"
     CDS             complement(8054..8527)
                     /locus_tag="ACG9CY_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS4937425.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8928..9152
                     /gene="nrdH"
                     /locus_tag="ACG9CY_000896"
     CDS             8928..9152
                     /gene="nrdH"
                     /locus_tag="ACG9CY_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS4937426.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            9311..11470
                     /gene="nrdE"
                     /locus_tag="ACG9CY_000897"
     CDS             9311..11470
                     /gene="nrdE"
                     /locus_tag="ACG9CY_000897"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS4937427.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            11520..12485
                     /gene="nrdF"
                     /locus_tag="ACG9CY_000898"
     CDS             11520..12485
                     /gene="nrdF"
                     /locus_tag="ACG9CY_000898"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS4937428.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(12574..13713)
                     /locus_tag="ACG9CY_000899"
     CDS             complement(12574..13713)
                     /locus_tag="ACG9CY_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4937429.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(14022..14735)
                     /locus_tag="ACG9CY_000900"
     CDS             complement(14022..14735)
                     /locus_tag="ACG9CY_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS4937430.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14989..15690)
                     /gene="nagB"
                     /locus_tag="ACG9CY_000901"
     CDS             complement(14989..15690)
                     /gene="nagB"
                     /locus_tag="ACG9CY_000901"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS4937431.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15924..17456
                     /locus_tag="ACG9CY_000902"
     CDS             15924..17456
                     /locus_tag="ACG9CY_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4937432.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(17647..18849)
                     /locus_tag="ACG9CY_000903"
     CDS             complement(17647..18849)
                     /locus_tag="ACG9CY_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4937433.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(18931..19620)
                     /locus_tag="ACG9CY_000904"
     CDS             complement(18931..19620)
                     /locus_tag="ACG9CY_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4937434.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(19726..20094)
                     /gene="rplL"
                     /locus_tag="ACG9CY_000905"
     CDS             complement(19726..20094)
                     /gene="rplL"
                     /locus_tag="ACG9CY_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS4937435.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(20147..20650)
                     /gene="rplJ"
                     /locus_tag="ACG9CY_000906"
     CDS             complement(20147..20650)
                     /gene="rplJ"
                     /locus_tag="ACG9CY_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS4937436.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(20854..20981)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(21060..21749)
                     /gene="rplA"
                     /locus_tag="ACG9CY_000907"
     CDS             complement(21060..21749)
                     /gene="rplA"
                     /locus_tag="ACG9CY_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS4937437.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(21853..22275)
                     /gene="rplK"
                     /locus_tag="ACG9CY_000908"
     CDS             complement(21853..22275)
                     /gene="rplK"
                     /locus_tag="ACG9CY_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS4937438.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            22618..23286
                     /gene="sdaAB"
                     /locus_tag="ACG9CY_000909"
     CDS             22618..23286
                     /gene="sdaAB"
                     /locus_tag="ACG9CY_000909"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS4937439.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            23299..24171
                     /gene="sdaAA"
                     /locus_tag="ACG9CY_000910"
     CDS             23299..24171
                     /gene="sdaAA"
                     /locus_tag="ACG9CY_000910"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS4937440.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(24262..25362)
                     /locus_tag="ACG9CY_000911"
                     /pseudo
     CDS             complement(24262..25362)
                     /locus_tag="ACG9CY_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(25454..26341)
                     /locus_tag="ACG9CY_000912"
     CDS             complement(25454..26341)
                     /locus_tag="ACG9CY_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4937441.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(26418..26963)
                     /gene="nusG"
                     /locus_tag="ACG9CY_000913"
     CDS             complement(26418..26963)
                     /gene="nusG"
                     /locus_tag="ACG9CY_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS4937442.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(27064..27234)
                     /gene="secE"
                     /locus_tag="ACG9CY_000914"
     CDS             complement(27064..27234)
                     /gene="secE"
                     /locus_tag="ACG9CY_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS4937443.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(27258..27410)
                     /gene="rpmG"
                     /locus_tag="ACG9CY_000915"
     CDS             complement(27258..27410)
                     /gene="rpmG"
                     /locus_tag="ACG9CY_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4937444.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            27572..28216
                     /gene="cbpA"
                     /locus_tag="ACG9CY_000916"
     CDS             27572..28216
                     /gene="cbpA"
                     /locus_tag="ACG9CY_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS4937445.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
BASE COUNT         8610 a         5794 c         4908 g         8943 t
ORIGIN      
        1 gattagcgat ttcttttaaa ctcagcaata aatttttata gtcatgtcta aatctggcaa
       61 atttttcttg atctttttcc agtgaatcag tatatttttt tagataaacc aattcttttc
      121 ttttcaattg ctgttcatat tgttctcgtt gcctaatagt tattctaata aataaaaata
      181 gaacaacaaa catttgaatg attaaaaata ttaagacacc aaacacaaaa tgatcaaata
      241 cttgatattg atgagcaaca taagaaacaa ataaaccaat tgtaagtaaa taaatcatga
      301 atatagcagt aagtttagaa ctggactgtt caacaatcct atttatagct aattttctat
      361 ataaccaaat aaacatcagc aaaaacaaag aatcaagcat tatctggaat actacataac
      421 catacccttt tatcccttca aatgaaaaca tatacgtcat aaccatggaa gtaataatag
      481 atctgaaatc atcaataagc atacataata gtaatttatt taaaagcact atattagttt
      541 tcttcttttc ctttaatata aaataacttc cgattatagg tatagctgcg gaaacatcag
      601 taaataattc aacaaaaaaa gtgagtggta tcaataaaca tacaaaaaga tattttcttt
      661 tgttaagatc agtattagtc atataaaata agagaaaaaa gaaaaagttt cccacgaaca
      721 ttcttaatag ataagctttt gtatcgatag taatcaagag atcccctcct gattttagga
      781 tatcataaaa aaaattttag ttacactttt taaataatga aaaaacacat tccactaaat
      841 ttgatgccgg ttttccttga ggttttgttc cagcacctcc tttaattttt ttaagctctt
      901 gatctgaaca ttgtttagca tttagtctat ttttttcttc cattacgatt ccccctacaa
      961 actataaata cttttatgtc actgattcaa tatacctatt tttctaatca aaaaattttg
     1021 agacatattt aatattataa gtatcattaa aattgagatt tatctccata atctgctcga
     1081 tataaaccat ttattccagc agttcttcca atttcactca aatttcttat taattcctct
     1141 tgatattcag tgaatcttat tttattagta taagcagtaa aatatagata attcaccaat
     1201 ctatttatta aaggaccggg atctaattca tatttttgtt tttctaattt taaatatacc
     1261 ttttgtaaaa cctcaagaat atcagaatat ttaggatctt tacttataga tatatcatta
     1321 tataattcat gaacaatttg cttagcattt tttttcattt tagcacttcc ctggaattgc
     1381 tccacctaaa aaaccaccta tagacattcc tgcaatacaa gtagttgcct tggcccaatc
     1441 gaccgtacat ttatttttag tgcaatacac tccatttcca taatattttc cactatgagt
     1501 ggtaccccca tagataagtt gtgtctcttt aatagacaaa atttttaaat gtttcataat
     1561 ctaatcaccc ctaaattaat gtttttagac tattattcta acattcatta aatttcaaaa
     1621 aacaattcct tcttgaaacg tagcaaaata attcctgaat aagaatttaa tcacttttaa
     1681 gtatataaac tatgtatatt ctttaatatt ttttgagaat gcacttcgta cataataaac
     1741 tttaatcaca ttagtctttc atacctacga tcgatctctt tgactcgcac ccataaattc
     1801 ataatagttt gtcggcgtct ttggacgttt cgaagatgag ctacgcattc tcttttttta
     1861 tagacaaaaa agatagagac gaacatggag cttccaaatt tggatatttg atccaatttt
     1921 ggaagctcca tttatttcgt gtctgtcttt ttattttttc cagctttcga ctaattcttg
     1981 gttctctttg atccagtctt ttgctgcttg ctgtggatct tttccattgt tgatttccaa
     2041 cataactttt tccatatctt tttctgacca atggaaattg tctaatactt tgtaagcttc
     2101 tggctggtct tctttcaatc cttttcgaac catcgtatgg atctgctctt cgcttcccat
     2161 tccgttttca ggatcttcta agtatttcag atcatatttg gcaaacatcc aatgaggtgt
     2221 ccatcctgta actacaattg gttcgtgttt tttgatcgct tgacctagtg caacggtcat
     2281 tgctcctgaa gaagatgtct ctactttcca gtctttcaag ttgtcatatt tttggatcgt
     2341 gttttcagca gccgtaacta caccagctcc tggctcgatt cctatgattt tcttacctgc
     2401 ctgatcggtt aactcatcga tcgaatttac atccatgtag gctggcactg ctaaaccaac
     2461 ttttgctcct gtcaaattgg cacctaggtc ttcgacttga tctttatatt gtgcatattg
     2521 tgaaccatga gttttaggta gccaagctga aaccatggca tctgactcgc catttgaaac
     2581 agatttccac atgattgaat tatctaatgg cgtcatcgtt acatcatagc ccatttgttt
     2641 caatacttca ccgactacat ttgttgaagc aacttctgta tcccattcaa cataagataa
     2701 gttgatacgt tttgtttctt tggcagatga aaaactccct ataccaatcg cacctaagat
     2761 cactactact acagcaccaa tgatcagtcc ttgacgtttt ttacttttct gtgttgttgc
     2821 tcctgcagct tttttattgt ttggctggtt caaatgctgt gtaaaacggt caatgatgat
     2881 tgctaagata actaatgcaa ccccgttgac aaatccattc ccgacttgtg ctctttgcaa
     2941 tgctgataat acgccgcgtc ctaagccggg tgcaccgatc atagaggcaa taacgaccat
     3001 tgaaagtgac agcattgttg tttgattgat ccctgccatg atcgtacttt tagctaaagg
     3061 caattctagt ttgaatagtt tttgacgact tgtgctcccg aatgaatcag aagcttctac
     3121 taattcttta ggaacttgtc gaatccctaa attcgtaaaa cgaacggttg gtggcagagc
     3181 aaagatgact gaggcaaata cccctggaac catcccgata ccgaaaaatg caactgctgg
     3241 aatcaaataa acgaatcctg gcattgtctg cataaagtca aggatcggtt tgataatact
     3301 ttgggcttta ctgctttttg ccatcaagat ccctaatggt accccaatga tgatcgagat
     3361 cacactagat aaaagaacaa gtgtgacagt attcatcaaa tcattccata gaccttggtt
     3421 ataaataaac catagaccga ttaatgtgaa caacgtaagt cctggacgtt tcttagaaat
     3481 aaagaaagct gctaccgtca ataacaagat aaacaaagga gctggaatca ctaacagtaa
     3541 atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt aaaaatgaaa ataggccaga
     3601 aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag tttgccactg gtaattgata
     3661 gttcaataga ttattcatgt tcactcacct cgcttgtttc tgctaatgct tcaatcacac
     3721 tacctcggat gacgacacct tttaattttc catcctctac aactgcaagt ggagtcggtg
     3781 agtcataaat aatattgaaa atatcactta ccaccatgtc tttcccaatc accgtcacat
     3841 tttcatctag gaagtcgatc aatggacgtt tttgcttcct agcttctaac gcttgatctg
     3901 cagtgatgat ccctttcagg tgtctctttc tgtcaacagc catcagcatg ctgacttctt
     3961 cattacgcat ccttgtcaat gcaacgtttg gtccatcaga ttcgatattc gtcgtcaatg
     4021 caggtaccat gatgttttga gccgtaagca ctttggaacg atcgacttct tccacaaatt
     4081 cacgtacata atcatttgct ggattggtca atatttcttc acctgttccg atctgcatga
     4141 tttcgccgtc tttcatcaag gcgattctgt ccccaatccg taaagcttca ttcaaatcat
     4201 gggtaatgaa aataatcgtt ttttgaacat tggcttgcag gtccagtaat tcgtcttgca
     4261 tttctcgccg gatcagcgga tcaagagccg agaacgcttc gtccatcagc aagatctcag
     4321 gatcatttgc taaagctctg gctaggccaa cccgttgttg catcccacca gataactgac
     4381 taggatattg gtctttaaat gaaagcaaac tagaattttc taatgctttt tctgcttttt
     4441 cttgtcgttc ttctttaggt acaccacgga cttctaaccc gtattctgta ttttccaaga
     4501 tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat attgatttta tgacgacgga
     4561 cttctcgcaa ttcttctttg cttaacttcg atacatcttc tccatcaata tagatactgc
     4621 ccgaagttgg ctcaatcaga cgattcaata gacggatcaa tgtggatttc ccactcccgg
     4681 aaagccccat gatcacgaag atttctcctt cttttacgtc aaaattcacg tcatacacac
     4741 caaccgttgc accggttttc tttaaaatct ccgttttcgg ctggtgatca ttcatcatat
     4801 ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa atgttttact tctactttta
     4861 ccaaacaaat tctccttttt tatgtaaatt catttttctt tctgaaaaaa gtgaccacac
     4921 tatgttaaca tcatgtagtc actttgccaa aaatgaaaga aatttgcttg tccaaactga
     4981 aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc aagttaatat ttttttaata
     5041 tttcacacaa tcattctgca agctgatgct ccgggtaaaa aaagtttgtc atacgctgta
     5101 gtgtatattc atttccaata aaatgaacga tgtcccctgc cgtaaattta gcataggggc
     5161 caggtgaaac aagcagttct tcattatgag aaattgcgat aactgtggcg ccagtcgcct
     5221 gccagatatt caactcgcta attgaacggt ccagatgcat tgctttttct gataaagata
     5281 attcaaacgg aagtaaagga ttcatcttat gtattcgctt cgtttgattg accagctgat
     5341 ccaataattg agaaaaatga ttcagctcat cttgttgctt ctccacactt gccatgattt
     5401 ctgcttttgt atcttggatg gattgtacat cttgatattg caataggaaa tcttttgctt
     5461 tttccttcga agcaacaaat gccccacttc catgccgtac ttccatgatt ccaagatcta
     5521 ccagcacatt gatagccttg cgggtagtct caggagaagc attgtacgta ctggcaagtg
     5581 ttgagcgggc atggatcttc tcacccactt tatatcggtt ttcaacgata cgttcggcta
     5641 aatcaacagc aatctgctgg tagcgcgggc ggacgatttt tttctttcca gacattttct
     5701 ttcctctttc tttttttact ttccctcatc ttactatgct ctagcaaaat aagcaaaaaa
     5761 ggggagaata gcctcttgca acgactgatc atcgcttgat ctgttccttt tttactgggc
     5821 aagctgtttg acagtcatca caattcttac cggattttaa acgagtatag acaattgcgc
     5881 ctgcacttcc aaagattaac acacttaata agaatgtcgc catcctattc ctctccttct
     5941 aatgacaata tagtataaaa ctggttcttg gattttggct gacggaacaa aacataaacg
     6001 agtcctatca tcagtaaaat agcaagatac gtccctaacg ctacctgtcc accctccaat
     6061 aagtgtccca actgataaat cacaaaacta actaaataag ctaaaccgca ttgatagcct
     6121 atggcgatcc aagtccattt cttggcattc atttcccgat ggatagcccc aattgccgca
     6181 aaacaaggcg cacaaagtaa attgaaggct aacaatgaat aagctgctgc gggagtatat
     6241 gtgtgttgta gcacttgcca gatttctctg ccattttcgg atacttccgc atgaccaaac
     6301 aaaataccaa atgtgccaat aatattttct ttagcgacca atcctgtaat cgctgcgact
     6361 gttccttgcc agtttcccca accaagcggc tgaaaaacaa ctgccaggat tcgcccgaaa
     6421 aaggctaaga tactttggtc ttcagaaaca ggttgagcat aaaaattata agaagagctg
     6481 aaccagatca cgatactaga aacaaagata atcgttcctg cttttttaac aaaagacttg
     6541 ctgcggtcat aggtttgtct taggaccgct gaccattgag gaaaatgata tgcgggcaat
     6601 tccatgataa aaggagctgt atctccagca aacaaacgtg tttttttcaa gctgattcca
     6661 gagaaaataa ttgcagtcat accaagaaaa taggcagaag gagctaccca agattgatga
     6721 ggaaaaaagg cccctgctac taaggcaata atcggcaact ttgcagaaca aggcatgaag
     6781 gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt caatcgtgcg actggccatg
     6841 acgcctggca ctccacatcc tgtagcaatc agcataggaa tgaaggattt tcctgaaaga
     6901 ccgaatttcc tgaaaagtcg atccatgaca aaagcgatcc tcgccatata tccgcagtct
     6961 tccagaaagc tcaagcataa aaacaatata atcaattgcg gtagaaatcc taacactgct
     7021 cccacgcctg cgatgatgcc gttcaagatc agctcctgca tccatcccgc tacttgccat
     7081 tcagctaaca atctgccaac atgttctggt aaccatgtac caaacaatgt gtcattgatc
     7141 caatctgtcc ccattgttcc tactgtttga atagccagat aatagatcag ccacatcaca
     7201 aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa tctgatcgat tttgtcactc
     7261 atcgataatt ggaaagaatc atttgacaca acacagagtg tacatagttg ggtaataaat
     7321 tcataacgtt cattgaccag aatcgtctca ctatcgtcgt ccaatagttt ctctgttaac
     7381 tggatcagtt gctcgatctc tttttgctga gaaactgcta tctcgtaatc ctctcttacc
     7441 ttctgatctc gttcaaaata tttcattgca taccagcgtt tcttgccgct ttgttctcct
     7501 agtacatctt caatttcatg taaagctgct tctaaacgtg gatcatatgt acagggaaca
     7561 ggaatagaag gccgcttttt tgcagactcc tgaatctttt cgattagttt ttcgattcct
     7621 tgctttttca atacgctgat tggtaaaact ggtacaccaa gaccatagga aagcttttct
     7681 agattgattt tttttccttc ttttttgatg acatccatca tattcagacc aatgatcata
     7741 ggaatgtcta aatccaataa ctgcgtagtt aaataaagat ttcgttctaa attggtagca
     7801 tcaataatgt tgatgatcag ttcaggctgt tcctgaacca aataatctct tgcaatcact
     7861 tcttctggtg tataaggaga caaagaatag atgccgggta ggtcctgaat atgaatgttc
     7921 ggattttttt tcaacgatcc ttcttttttt tctactgtta cgcccggcca attccctaca
     7981 tattgattcg aaccagtcaa gagattgaac aagctagttt tcccgctgtt tggatttccg
     8041 actaacgcga tattcattct ttttcctccc tttgtatcaa gattagttcg gcctctgact
     8101 ttcgtaacgt cagttcatac cctcgcactg tcagttcgat tggatctccc agaggggcta
     8161 gttttcggat aactattttt acatttcgcg tcaatcccat atccattaac cgtcgcttca
     8221 ctgcgccttg tccgtggatt gctacgacga tcccagtctc tcctgctttt aattgatcaa
     8281 gagaagtcca gctttcagag gaagcagtct ttttattgac aaagatttga cctagtattt
     8341 cccgacttac tgccactcga tgacttttta ccagtaaaat ggcattgttg cctgataaat
     8401 tgacgacgac gattttactt ccggcaacga tcccgatatt ttgcaaatgg cgttttgttt
     8461 tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt ctgctttgcc tgcgctaagg
     8521 tctgcatatt gattcctctc ttgtgaaata aatcatgtat taaaatcatt cattatatgt
     8581 tctaattttc ttacgtgtta cgcaaataaa tgagaatcat tctcacatag aatactctcg
     8641 gtaattgaga atgtcaatta tacttgtgat attatgagcg aatctttagt attgatagat
     8701 aaaaatgaga aaaaaatatt tcttcatagt tttccacaat tttttctcta ttaaatttgc
     8761 tattttccca cacgatactt gttttcagct cttttttcaa aaacacaaaa cttagtattc
     8821 cacagtttcc acaacacaat atgtagtgtt tgttgtttga cttttttact aagagtttta
     8881 tactatctat tatagataat aagaatgtgt tagaggaggc actcaccatg aacgtaaaaa
     8941 tcttttctaa aaataattgt atgcaatgta agatggtcaa acggttttta acagaaaatc
     9001 aaattgcttt tgaagaagtc aatatcgatg aacagcctga agcagtagac tggttgaaag
     9061 cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc agaaacagtt atcggattcc
     9121 gtcctgatca gttgcgtaaa cttgccagct aacatgacta aaagaatcaa aacgtgatac
     9181 atggatttga agggcttgag acagcaccag tttttgtcgc aaacttccaa atcctttttt
     9241 aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca tcaaatacga aatgaaggtt
     9301 gggcgtacgt atgagtctta aaaatttaca agatgtcagt tatttcaagc tgaataatga
     9361 aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag gataaagaag ctttagttgc
     9421 cttttttgag gaaaatgtca aaccaaatac gatgactttt ctatctatta tggaaaaaat
     9481 caattacttg atcgatcaag aatatataga agcagaattc attcaattgt attcaccatc
     9541 atttattgaa aagctatatc aatttttgga cgagcaacat ttttctttca aatcttttat
     9601 ggctgcttac aaattttatt ctcaatatgc gttgaaaacc aacgacggtt ccgcttatct
     9661 ggaaacttat gaagatcgag tagccttcaa tgcgctttac tttgcgaacg gggatgaaga
     9721 attagcactt gctcttgcag acgaaatgat ccatcaacga tatcagccag ctacaccttc
     9781 tttcttgaat gctggacgta aacgtcgggg cgaattggtg tcttgtttct tgactcaagt
     9841 cactgacgat atgaactcta tcgggcgctc aatcaactct gctttgcaat tgtcccgtat
     9901 tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa gccggcgctc ctatcaaagg
     9961 atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa ttattcgaag atagtttcag
    10021 ctattctaat caattaggtc agcgtcaagg agctggcgtg gtttatttga acgttttcca
    10081 tccagacatt attgctttcc tttcagcaaa aaaagaaaat gcagatgaaa agattcgcgt
    10141 aaaaactctt tctcttggcg tgatcgtacc tgataaattt tatgaattga cacgtaaaaa
    10201 cgaagatatg tatttattta gtccatatag tgtcgaaaaa gaatatggtg tcccattctc
    10261 ttatgtaaat attacagctg agtatgatca tctagttgca aatccaaaca ttcgcaaaca
    10321 aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa ttacaacaag aatctggcta
    10381 tccttacgta atcaatattg atacagcgaa ccgcgagaat ccaatcgatg gcaagatcat
    10441 catgagtaat ttatgttcag agattttaca agttcaaacg ccttctgtga ttaacggcaa
    10501 acaggaatat gaagtattag gaacggatat ctcatgtaac ttaggctcga caaatatcgt
    10561 gaatttgatg caaagcccta actttggtca atctgtgcgt acgatgactc gtgcattgac
    10621 atttattaca gatgcatctg atatcgatgt cgtaccaccg atccaaaacg gaaataagct
    10681 gaatcatacg attggattag gtgccatggg cttacacact tatcttgcaa aagaacaaat
    10741 ggaatatggc tcagaggatt cattagactt tacaaatatt tacttcatgc tgttgaatta
    10801 ttggacactt gttgaaagca acaatatcgc tcgtgaaaga aaacaagttt tccataattt
    10861 tgaaaaatca gcttatgctg acggcactta tttcgaaaaa tacgtaactg gagaattcca
    10921 gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt tttatcccaa ctgctgaaga
    10981 ttggaatgct ttgaaacaag cagtaatgaa agatggactg tatcatcaaa atcgcttggc
    11041 agtagcacca aatggctcaa tttcatatat caacgatacg agtgcaagta ttcatcctat
    11101 cactcgcatg atcgaagaac gccaagagaa gaaaatcggt aaaatttatt acccagcgcc
    11161 ttatctatcc aatgagacga ttccgtatta cacttctgct tatgatatgg acatgcgtaa
    11221 agtaatcgat gtttatgcaa tggcacaaca acacgtcgat caaggcatga gtttgacttt
    11281 attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg aagacaacaa cgaagcaaac
    11341 gacacgtgat ttgaatattt taagacatta cgctttccac aaaggaatca aatccattta
    11401 ttacgtacgt acctttactg atgatgcaga agaaatcgga agcaatcaat gtgaaagctg
    11461 cgtgatctaa tatcatagat agattcgctt attcatattt taattaaagg agcattctca
    11521 tgtcagaaac atattatgaa gcaataaact ggaatgaaat tgaagatatc attgataaat
    11581 ccacttggga gaagctgaca gagcaatttt ggctagatac acgtatccca ctttcaaatg
    11641 acctagatga ttggcgtaca ttgtctcaat tggaaaaaga tacagtagga catgtatttg
    11701 gcggtttgac actgctagat actgtccaat ctgaaagtgg catggatcag ttgcgtaaag
    11761 atgtccgcac tccccatgaa gaagccgttt tgaacaatat tcaattcatg gaatccgttc
    11821 atgccaaaag ttattcttct atttttagta cactgaatac gaaaaaagaa atcgaagaaa
    11881 tttttgactg gacaaatacg aatccttatc tgcaaaaaaa agctgagcgt atcaatgaaa
    11941 tctacaaaca cggtacaccg ctagaaaaga aaattgccag cgtcttttta gaaacattct
    12001 tgttctattc aggtttttat actcctcttt attatttagg aaacaacaaa ttggctaacg
    12061 tagcagagat catcaaacta atcatccgag atgaatctgt acatggaacc tacatcggtt
    12121 acaaattcca attaggattc aacgagttac ctgaagcaga acaagaaacg ttaaaagact
    12181 ggatgtacaa tcttctttat gagctttacg aaaacgaaga acgttataca gaagaattat
    12241 acgatccaat cggctggaca gaagaagtca agacattcct acgctataat gccaacaaag
    12301 cattgatgaa cctagggatg gatccactct ttccagatac tgccaatgat gtcaacccaa
    12361 tcgtaatgaa cgggatttca acaggcacaa gcaaccatga cttcttctca caagtcggaa
    12421 acggctattt gttaggtcat gtagaagcga tgaaagacga cgattatttg attggattgg
    12481 aataaggatt tattttaatt caaaaatttt tcgaaaaata aaaagagttt agcgtacaga
    12541 tattttaaat gtacactgaa ctctttttta tgcttattca cttgtttctt ccagcacttt
    12601 tggactgtct aacttttccg aaacatgttc cattcttctc aataaataga ttttccggat
    12661 atttaatatt atcgtcttaa tcactgcata gacaggtatg caaatcagca tgcctaatat
    12721 tcctgcgaca cttcctgctc caatcaatac caagatgatc gtcaaaggat ggatattcat
    12781 tgatttccca aataacaacg gcttgatcaa atttccatct aattgttgga tcactaaaat
    12841 cgacaacgcc atatacaaag cttgaaatgg agagataaac aaaccgacaa tgattgctgg
    12901 taccgctcca ataaaagggc cgacatacgg gataatattc gtgattccgc aaatgaccgc
    12961 aagcaacaag ctataaggct gatcgaatat tctcattgcc aaaaagctca ttacaccaat
    13021 aatcaaagca tctaagatcg tactgctgat ataggcagat aatgtttcgt ttaattcttt
    13081 taccgtttgg cgtaactctt tacgaatcgc tttcggaaag aagtgagaca acgcatcaat
    13141 aaatttgtgc ccatccttga acataaatag caaaataaat ggaacggtaa ataacaaaat
    13201 aaaaaattgc atcataaatg aaatgatttt tgagacactt gcagataaac tgactaatat
    13261 gatattgaac aaattactaa gagataaatt tgctgatgct aattgttctt ccaaattgaa
    13321 atttttaaac tcttcccgct gcgccaattc atttaaccat ttgcttgttt catctgcata
    13381 cattggcaag ctctgggtca attgaattgt ctgttccatt aactgaggca ctacattcat
    13441 gacagttaaa acaactaaaa tgattacgac caaaaacgtc aataaaaaac cgaacactct
    13501 cgggatccga tgcttttcga gaaaaacaac gattggatcg aacatataat agataaaacc
    13561 agcaacaagc aatggtaaaa aaactgccga aagcattttc aatattggac taaaaatatt
    13621 cggcatttgt aaaataaagt acacgccgat gatcgtgagt actagctcca ctgtccagaa
    13681 aaataatttt gattgtttaa aacgtgattg catacatctc ctccaaggtc ctttgatact
    13741 tctattctat catttaacaa agagttacgc gctgagattt tcatttgctt aaaaaaaaca
    13801 aaaaaaataa acatatttta atgagtagac gagtacttaa acaaaaacac ggtttggagt
    13861 agtgacaaaa attatactgc agtccctttt tccaaataaa cggtgttcaa aagcttgcat
    13921 cttcgtaata agcccagcca aacgaaaaat ataaggaact atttttattt ggctgggctt
    13981 attacttgat gtaggacatc tattttacaa cctcagatca tttattgcgc aatactgact
    14041 aaatgcaact ttgtgatatt tttgtgtacg gacaactcct cgatcacatc ggcataagtg
    14101 agcccttttg gaagatcaat cgtatagata tttttgtaca ctcggtagtc atcaacgaat
    14161 tttacgtcaa aattcacatc ttcgattacg atatttttct cttcaaaata atcggtaaga
    14221 aaatctttgg tttcttttct atggataaat tggatttcta actttttagt cgtaggtaca
    14281 tggatcacgc gtttcactac tgtgagtgca atagcaatcc cgataaaact gatgatcgca
    14341 atggaatagt aacccatgcc gatggcgatc cctaatccag caattgccca taaagaagcg
    14401 gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga tcgttcccgc gcctaagaaa
    14461 ccgacaccac tcaccacttg agcaatcagc cttgcttcat ctgaacgaat cgttccagca
    14521 agctgtggat actctaaagc atctcttaat gcaccactag cgatctcaac ttggatcaat
    14581 gcaatgatac atgctcccat acaaactaaa atatgcgtac gcatacctgc tggacgattt
    14641 ttatattgtc gttcaaaacc gattgctccg ccaaatagca tagcgatccc taaacgtaaa
    14701 ataatctctg gaattgtcaa taccatatca ttcatctaaa caccctccag tccaatatat
    14761 catgtctgaa aggagcagac aaaaaaagtg caatttttac ctcttacgaa aaaaggactg
    14821 tgacaaaatt tttgtcatag tcctttttat gaataaacgg tgttcaatca gctgaccctc
    14881 ggtattactc aacaaacggc aaaatatggg gagctatttt tcctttgtct tctctaatac
    14941 tcaggtcaaa acgctgattt cacaacctct ttagcctact attgaaaatt acaacttact
    15001 tgctgcttct tcatcgatga tcacaacaac attgtcgtga ttttgcaata cacttgctgg
    15061 caaatgattc gttacaggtc cttcaatcat ccctttgatt gcatcagctt tgctttcgcc
    15121 aaatgccatc aagacgattt ctttactttt cataattgaa ccgattccca ttgaaacagc
    15181 ttttgttggt acatcttctg ctttatcgaa gaatcgtttg tttgcattga ttgttgattc
    15241 tgttaagtca actactgaag tcgttccgtc aaatgaagca ccaggttcat tgaaaccgat
    15301 atgtccattt tgcccgattc ctaagatttg gatatcgatt ggatgtgcat cgatgatttg
    15361 atcatacttt ttgcatgctg cttccagatc ttctgctttt ccatctggta caaacgtttc
    15421 tttgaatggt tttttatcaa ataattggac atccatgaaa tgacggtagc tttgttcatc
    15481 tgaaccgcct agtccaacgt actcatctaa gtttacagaa gtcatttcac tgaaatccaa
    15541 atcgcttgaa gtcatttctt tatataaagt aactggtgtg cttcctgttg ccaatcctaa
    15601 tacttttgca cctttgttca taccttcttt gatgatttca aatgcttttt ttccgccttc
    15661 ttcagcgttt tttacacgaa tgatttccat agtgattgct cctttatatt ttggtatagc
    15721 ccaattatag cattcttttt ttctttttac aataattagt atagaccaaa ttgcattttt
    15781 ttcattttct tgacaataaa taggaatccg tgttaggttt cataagatgg ttcaatcaaa
    15841 caatccatgt tgcatgaaaa ccaagcaagt gattcactgt ggacgcttta tgtctgcagt
    15901 ttttttatga aaaaggagtt attatgagta tactgacatt aaacaacatt acccagcaat
    15961 ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt aaatgctggc gaacatcttg
    16021 ggctgattgg acaaaacgga gccggtaaaa gtacattgat taaaatcatc actggtgaga
    16081 tcttaccaga tgacggacag atccattggc aaaaaaatat tcacaaaggt tacctcgatc
    16141 aatatgtgga agtcgatgaa acattaacaa tcgaagaatt tctaaaaact gcgtacgcaa
    16201 aagaatacga aaaggaagaa aaaattgctt ttctttacca aacatatagc gagaccttcg
    16261 atgattcctt gctggaaaaa gctgggaaac tccaaacgga attagaccaa ggagtgtttt
    16321 atcaaatcga caccttagtt gctgagatga gcagcgggtt ggggatcgat gtcttaggtt
    16381 tagattcact gttgaaaaat ctaagtggtg gacaacgttc aaaggtgatt ttagcaaaac
    16441 tacttttaga aaacccagat gttctgatct tagatgaacc aaccaatcac ttagatgatc
    16501 agcatatcca atggctgacg cagtttcttc aggattttga cggtgcgtat atcgtgattt
    16561 cccatgacca agaatttctt gatcagatca ccacccatat cgcagacatc gagtttggaa
    16621 agattactaa gtatactggc catctaaaac aagcattgaa acaaaaggaa caaaatcgcg
    16681 aaagctattt gcgccaatat catgctcaac aaaaacaaat cgaaaagacc gaagcttata
    16741 ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa aagccgacaa aaacaattag
    16801 ataaaatcga acggctgacg cctccagctt cagcaagtaa agctgtcttt gattttccat
    16861 tctcgcctat tgtcacgaca ttagcagtgg aaacaactga tttagtcatc ggatatgatc
    16921 gaccgctact tgcacccatc aatttgacca tccgctttgg tgaaaatatt gccattcgag
    16981 gcttcaacgg gattggaaaa tctactttat taaaaacact gatcggtgag attgaaaaaa
    17041 taagtggcga tttccacttc ccagataaca caaaaatcaa ttacttttca caagatctgt
    17101 actgggaaaa tccattagaa acaccactac aatatttaag cagtcaattt ccaaaagcga
    17161 ctatcaaaga attacgtcgc caactagcta aagcagggtt agttaatcaa ttagcaagtg
    17221 aacctctcac tacattaagt ggtggggaac aaactaaggt gaaacttgca cagttgacaa
    17281 tgaaccaagg taatttactg attctcgatg aaccgacgaa ccatatcgat caagaaacga
    17341 aagaaagctt acaagacggg atacagaaat ttccaggaac agtaatcatc gtctctcatg
    17401 aacaagaatt ttatcaagat cttgtgcaac gagtcattga gattgaacca aaataaacgt
    17461 ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt ttgtcacaac tccttcttct
    17521 gaataaatgg tggccaatta gctgaccttc ggtattagct caaaaaacgg caaaatatga
    17581 ggagctattt ttatttggct attcttatta cttgatgcag aacagctttt cacaacctct
    17641 tttcgtctat tttttatttt cgagatttct ctttccttta aaaacaactg tttgaaagaa
    17701 gaacgcacat ccttgaaaaa caagcccgct aaccgttaca cctaaccagc catactgctg
    17761 ccacattgtc gtccctagca atgaaccagc cgcacctcca ataaagtaca taaacataaa
    17821 tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg tttgattggc
    17881 gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca aaatggccaa
    17941 atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa gtcccatata
    18001 cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg cagcaactgc
    18061 tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat tgtaggcggg
    18121 actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa acatgaaaaa
    18181 tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg gcaaactttt
    18241 aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg gcattttcca
    18301 gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga tcatccgcca
    18361 agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc ccacgagtaa
    18421 accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc
    18481 gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag aagcgcatgc
    18541 aaataacgta aacgaaggcg caaaaaaagc ggccaacaga gagacggcag ataacgctgc
    18601 cattcgaatg atcaattttg gacgatccac cacgtcccct aaaggaacta aaaacagtaa
    18661 ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac
    18721 agataaacta gctgctactt ttgttccaat cggctggatg taatacatat tggcaacaac
    18781 aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtt ttttttcatt
    18841 actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa aagattggaa
    18901 atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc cttgtttttt
    18961 cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac ttgcaaccaa
    19021 cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg catacaacac
    19081 agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag gcaatttcat
    19141 cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat agcctaacat
    19201 tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat tcgtttcggt
    19261 tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt taccaagagc
    19321 attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac cagcaatgac
    19381 tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca atacgatcaa
    19441 gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg ccttactcgc
    19501 tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc gatagcccac
    19561 taccagtatc agaatgattc ccaacgagat caatatcaat cggattattt tctttttcat
    19621 atttcctcct gaaaaaaatc tatattcatt aaataatagt ttaacataaa aacaaaaaag
    19681 caccgaaatc caacatagga tctcagtgct tttaaatagt aaaaattatt taacagttac
    19741 agatgcgcca acttcttcaa gtttagcttt caattcttca gcttcttctt tagatacgcc
    19801 ttctttaact ggtgcaggag cgccatcaac tacagcttta gcttctttca agcctaaacc
    19861 agttgcttca cgaactgctt tgataacttt aactttttgg tctccagctg cagttaattc
    19921 tacagtgaat tctgtttgtt cttcagcagc agcagcgcca ccagcagcag ctacagctac
    19981 aggagcagca gcagatacgc caaattcttc ttcaatagct ttaactaggt cgttaagttc
    20041 taggatagtt gcttctttta actcagcaac gatgttttca atgttcaatg ccattataat
    20101 ttcctccgtt taattgttct tttttattta atgctttatg cggcaattaa gctgcatctc
    20161 catcttcttt gtctgccact gctttgacag cgtaagccac gttgcggact ggcgcttgta
    20221 gtacagatag caacatagat agaagtcctt cgcgatttgg caattttgcc aatgctgtga
    20281 tttcttccaa tgaagagact ttaccttcga tgataccgcc tttgatttct aatgcttttg
    20341 cttctttagc aaattcgtcc atgattttcg ctggtgcgac tacatcttcg ttactgaatg
    20401 caacagctgt aggtccagtg aatacttcgt ccatgccttc aaggccagct tttttagctg
    20461 cacgtgaaag aatagaattt ttgataactt tcatttctac gttagcatca cgaagttgtt
    20521 tacgtaaacg tgttacttca tcaactgtta atccgcggta gtcaacgact actactgatg
    20581 cagctgctgt aaatttttcg cttacttcat caacgattgc tgcttttttt gcgataattg
    20641 cttcactcat ttttgagttt cacctcctga attttgatgg aagagttttt tgcactgaaa
    20701 aggcaagtca cttttatagt tactttgctc aaccagtatt attcatcacc tattcacttc
    20761 gttcatagtg attctactcg ctttgctcga aaggaaactc ctttcgcttt gctcaaccag
    20821 tattgttcat catctcttca cttcgttcat agtgattcac aataaaaaac tctatgccac
    20881 cgcagacata gagggactat tgaatatatt caataaacgt cctcggtagg aaattaaggc
    20941 ttgatgccac ctactgtctt cggtacaagt taattattaa cctcaactag cttagcatgc
    21001 taaactagtt gagtcaagaa ttatttttca gttggatcaa tcaacattct aatatttaat
    21061 tagaaagaag cttggtcaac atggatacca ggtccaaatg ttgttgtgat cgttacattt
    21121 ttcatgtatt gacctttagc tgctgatggt ttagctttta gcaatacatc atggattgct
    21181 gcaaagtttt caactaattt ttcatcgtca aatgaaactt ttccgattgg tacatggata
    21241 tttccagctt tgtccacacg gtaagttact ttaccagctt tcacttcgtt tactgctttt
    21301 gttacgtcca ttgtaactgt accagtttta gggtttggca ttaagccttt tggtcctaat
    21361 actcgtccta gacgacctac agtagccatc atatcaggtg ttgcaacaac tacatcaaag
    21421 tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt cacctacgaa atctgcacca
    21481 gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa ctaaaacagt ttgtgtttta
    21541 ccagttccgt ttggtaatac aacagcacca cggatttgtt ggtctgcttt tttagggtct
    21601 acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt ttgcaatgtt tgtttctttt
    21661 gctaaagcta ctgcttcggc aacaggataa actttacttg aatcaacttt ttttaatgca
    21721 tcttgcattt ttttgctctt tttagccatt ttggttcctc cttgattgtg gttataacgg
    21781 ttgtacctcc cacgtgttct atatctttca atatagagcc aactgagaag ctgctgtctt
    21841 agggtgtata gattattcta cagtgatccc catgcttcgt gcagttcctt caaccatgcg
    21901 cattgctgct tcaacgttag ctgcgtttag gtcttccatt tttaattcag cgatttctct
    21961 tacttgatcg ctagaaactt tcgcaacttt atttttgttt ggttcgcctg aacctttttc
    22021 gattttagct gcttttttca atagaacagc agctggcggt gtttttgtaa cgaatgtgaa
    22081 tgaacggtct tcgtatacag aaataacaac cggaatgatc aaacctgctt gatcagctgt
    22141 acgagcgttg aattcttttg tgaatcccat gatattgata cccgcttgac ctagtgcagg
    22201 acctactggt ggagctggag ttgctttacc tgcaggaatt tgcaatttaa cgattttttc
    22261 tactttcttt gccacgagac atacctcctt aagtccgtga tgtggttaat tggagatgca
    22321 tatttctcct cccacgtttt atctgatctg aaaaaatcca gacaaataat gtgcaaaaca
    22381 atgcacactg gtacagtata gcaaatttat ttgttttttc aagatttttt tctgctattt
    22441 ttacattttg cttgaataaa cgctaaattt attctaaaaa aacaacaatt tttcgttctc
    22501 atttcttctt ttatgttaca cttgcgctag catggttcaa cagatgaaag caaacaataa
    22561 cacggttttt tagacccttg tacaaagatt cctacataac aaaatgaggt ggagaaaatg
    22621 aaagatttaa ggtatagaag tgtctttgat attattggac ctgtgatgat tggtccaagt
    22681 agctctcaca ctgccggagc tgctagaatc gggaaaatca tacgtagtat atttggcgaa
    22741 cagcctgatt ctgtagatat ttatctatat gaatcatttg caaaaaccta tcgggggcac
    22801 ggaacagata ttgcattagt tggcggcctg ttagggatgg aacccgatga tgaacgttta
    22861 gctgattctt tgaaattggc atatgaagcc ggtatggaag tcttgtttgt tcctaagagt
    22921 gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa aaaaaggaac acgaaagctt
    22981 tcagtcactg gcatttcgat cggcggtggc aatatccaga tttctgaatt gaatggattc
    23041 aaaatttctt taagcatggg tactcctacc ttgatcgttg tccaccaaga tgttccaggt
    23101 atgattgcta aagtgactag tatactttct gagaccaata cgaatatcgg cactatgaca
    23161 gtgacacggg aatcaaaagg tgaaaatgcc attatgataa ttgaagtaga tgatcctcaa
    23221 gtagaagaaa cggtaaaaag actcaaacag ctgccaaata tcgacagcgt aaattatttc
    23281 gattaaggag ctaaaaaaat gtttttatcg attgaagaat tagtagatca agcaaaagct
    23341 tatccaagtg tagcagaatt gatgattgca gttgaaatgg aaatgagtgg ccgttcccgt
    23401 agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa tggaacaatc aatcaacgag
    23461 ggaaaagaag gtgttacctc cgtcacagga attaccggtg gtgatgcaaa aaaattagat
    23521 gattatttaa atgaggcaga ctttttaagt ggagaaacaa tcttaaaagc tgtacgcaat
    23581 gctgtagctg ttaatgaagt caatgcaaaa atggggttga tttgtgctac accgactgcc
    23641 ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc gagatcggct tgatttgaca
    23701 catgaacagc aattggaatt cttatttacc gctggcgctt tcgggttagt catcgcaaac
    23761 aacgcttcta tcagtggtgc agaaggggga tgtcaagcag aagttggttc tgcaagtgca
    23821 atggcagcag cagctttggt ttgtgcaaaa aatgggactc ctcatcaagc agcacaagca
    23881 gtagccatta cattgaaaaa tatgatggga ctgatttgcg atcccgttgc aggcttagtt
    23941 gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc aagcttttat ctctgctgat
    24001 atggcattag cgggtatcga aagtgtcatt ccgccggatg aagtcgttgc tgcaatgtac
    24061 caagtcggac gtcaaatgcc gtcgattttc aaagaaacag cagaaggtgg cttagctgtt
    24121 acgccaactg ctaaacgact tgcggaagag attttaaata agagggcgtg ataaagctcc
    24181 ttaatttcga gtattaagag attagttatc ttcataacaa aaaacgaaga gaggactaat
    24241 tgcccttctc ttcgtttttt attaatccat caatttcgta ttttttactt catcgatcgt
    24301 ttttgttcct gcaagttgca tcgtgatcga caattctttg ttcaggtgtt caaaaacaga
    24361 tgttactcct tctgccccac ctagattcaa cccataaatc actggtcggc caatcgcaac
    24421 taagtcagca ccacttgcca gagctttgaa gacatgctct cctcgacgga caccagagtc
    24481 gaaaatcact ggtacacgtt tgttgactac ttcggcaatt tttggcaata cttcaaagga
    24541 cgcaggacct ccatctagtt gacgtccacc atgattggat acccagatac catctgcccc
    24601 tgcagagata gcgattactg catcttctgg cgattgtatt ccttttacga taactggaag
    24661 atgggtgatt tctttgattg ttttgatgtc atctggtgtc aagccttgtt ttgcagctgc
    24721 ataaatttct gcgattcctt ttccttcgcc attaccactt gcactttgtt cgctatacgc
    24781 agctaagtta ggcattggta atggaaattg gaattgattg atgacgtctt cttcacggta
    24841 accaccgagc gttgaatctg ccgttaaaat gatagcttta gcccctgctt ctactgcttt
    24901 gttcaaaatg aactcattga atctgtcgtc tttactcata tataattgga aaaactgcgg
    24961 tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta tttgcataag tgctgatgga
    25021 aaagattgat ccagcagcag ctactccttt agctgtatca gcttcccctt tttcatgtgc
    25081 caatccttgg gccgctgatg gcgcttggat aatcggtgta tctaattcaa ttccgaaaac
    25141 agatgtatgc aaatcggctg agtcgattcc gcgtaatatc cgtggcatga ttttctttgt
    25201 attaaaagaa gtcgtatttt ctttcatcgt ccactcatct tcagaaccac cgcgaatata
    25261 gccaaaagct cctgcttcca tgcgctcttt tacacgacct tctagttagg caatgtttac
    25321 aatatcgatt ggatgttctt cattacttgt atgataagtc atagtagatc cttctttcat
    25381 cttcttgatt acatataaat catactaaac ttacaaaaag taagcaaaca tttactctca
    25441 gatactttta tcgctattct ttccaaataa atcgacttac aagctgatct acttcttggt
    25501 tttcatgaag catactatga gaggcatttt cacctgtcac tactttttcc tctatttcat
    25561 ttccgcgttg cttcaacaac gcatagaccg ctaatgcact gtttaacggt actgttccat
    25621 cactcaagtc tgtttcatct agttgaccag caatcaagaa aaatcgagtg gttataggaa
    25681 tattaccgat catttgctgg taatcttgat agcggttaga aacgacagca ggcccctttg
    25741 ctaactcatc tgtcagagac tgcgcactat catcgacaaa atcattaaaa ggcgcgccaa
    25801 ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg tccatacgtt cctaaatagc
    25861 gtagtgcact aactcctccc atggaatgcc ccactatgtt gactttatct atgccgtagt
    25921 tttctttcag atacaaaaga caagttttga tccattctgc ttgattccac tcgttatttt
    25981 tattatcttc aaacaatact tggaccatcg gattgtctgc ttttccagac aaatctcctg
    26041 ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa ttcttttact gcttgctgat
    26101 ttttttccaa acgccgcaac atccctttaa aggaattttc tgtcccgctg tagccgtgga
    26161 aaaaaagtgt tggaatagtg gttttttcta tcactttact ggattctgcc gttttttctt
    26221 tatcagaaac ttcagtagta tttatcttcg tcgtctggca accacttaac gcaaacaaac
    26281 tgataaggac taaagctgct gcagttgcac gtttgcataa atttttgatt ggttctgtca
    26341 tacacctctt cctttccata tagaaaaaag aaagacttga aaggccaatc atatgatcga
    26401 catttcaagt ctctttatta atcgatttta tctacttgat cgaaatcaag ttctgtactg
    26461 gtttcacgtc caaacatatc gatgttgact ttcaattttt ctttttctcc gtcaacttct
    26521 gttacttgtc cttctaatcc agcaaatgcg ccttcgatga ttttcacgac ttcgccaact
    26581 tctacttcta gatcattttg tcgcaggctc ataccgatag aacgcaagat atgattgact
    26641 tcttctggaa gtaatggtgc tggtttgctt ccggctccgt gtgaaccaac gaatcctgtc
    26701 acaccaggtg tgttgcggac aacataccat gaagcgtctg tcatgatcat ttcaacaagt
    26761 acgtatcctg ggaatgtttt atgaacgatt tctttttctt tcccattttt gacttcagtt
    26821 tctttttctt caggaacaac gacacggaaa ataaagtcgg ccattttcat actttgcgca
    26881 cgagattcga tatttgcttt tactttgttt tcatatcctg aatatgtatg caagacatac
    26941 cagttttttt caaaagattc cattgaaggc tcctcatttc attgtaaaat aaaaaaacct
    27001 cagtacaccc gaagtttttc tttcgcttca ttatagcact aaaagtacgt tgtggctagc
    27061 gagttatttt aaaatccagc caaataagct ttggatggct gtatccatta cgtaaaacat
    27121 caatccgaaa ataatagaag tttcgatgac tactaacgta tctttacgga gttgttttct
    27181 gcttggccaa gttacttgtt tgatttcatc ttttacgctt tttagaaatt tcatgagaca
    27241 acctcccttg ctactgttta tttcgtttct ttatgtaatg tatattgatt acaatattta
    27301 caaaacttat tgatttccaa acgttcgccg ctttttcctt cactgactga ttttgtgtag
    27361 ttgcgagacc cacaaacgga acaagcgagt gctgcttttt tctttgccat ttacttctgc
    27421 ccccttgctg agaatcagac ttatacttca taacagtatc acgtaacttt ttacttgtca
    27481 atttgtgaaa taaactatct attgttttgg ataaaattat gatatgatta atttgaaatt
    27541 aagctaaaat gagacaattg gaggtatagc gatgctatta aaaacacttg tatataagaa
    27601 aaaagatctg acaaccgtta cagagactag tacattagaa gaagcattaa aaattttaga
    27661 aacttccgga taccgttgtg tgccgatctt agattcttca ggtaatattt tccgtggaaa
    27721 catttacaaa atgcatatct atcgtcataa agcaaatggc ggtgatatga cgcttcctgt
    27781 cactcatctt ttgaaaaacg ctacaaagtt catttacctg gatacttctt tcttcaaagt
    27841 attctttacg atcaaagagc ttccttatat tgctgtcctt gacagtgaaa atcattttta
    27901 tggtattttg acacatagta cgttgcttaa tatgctctct caatcttgga gcgtagaaca
    27961 aggaagctat gtactgacaa ttgcttccac aggaaagcaa ggagatttgg ctgctattgc
    28021 aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt actcttgata tcgaaaaaga
    28081 cgaatacatt cgacgtacat tgatcacgct accggctaac actgaagcga aagtatgtga
    28141 aacgatcgtc aaccacttag agaaaaagaa ttataaagtg gtagaaattg aaaatttgca
    28201 aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag ctaatctgct ttctt
//
LOCUS       DAZULG010000022        27472 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000022 DAZULG010000000
VERSION     DAZULG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.022"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..964)
                     /locus_tag="ACG9CY_000917"
     CDS             complement(20..964)
                     /locus_tag="ACG9CY_000917"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4937446.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1260..2204)
                     /locus_tag="ACG9CY_000918"
     CDS             complement(1260..2204)
                     /locus_tag="ACG9CY_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4937447.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2425..3639
                     /locus_tag="ACG9CY_000919"
     CDS             2425..3639
                     /locus_tag="ACG9CY_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4937448.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3661..5676
                     /locus_tag="ACG9CY_000920"
     CDS             3661..5676
                     /locus_tag="ACG9CY_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS4937449.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5759..7132)
                     /locus_tag="ACG9CY_000921"
     CDS             complement(5759..7132)
                     /locus_tag="ACG9CY_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4937450.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7158..7442)
                     /locus_tag="ACG9CY_000922"
     CDS             complement(7158..7442)
                     /locus_tag="ACG9CY_000922"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4937451.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7444..9459)
                     /locus_tag="ACG9CY_000923"
     CDS             complement(7444..9459)
                     /locus_tag="ACG9CY_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4937452.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9638..9943)
                     /locus_tag="ACG9CY_000924"
     CDS             complement(9638..9943)
                     /locus_tag="ACG9CY_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS4937453.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10032..11063)
                     /locus_tag="ACG9CY_000925"
     CDS             complement(10032..11063)
                     /locus_tag="ACG9CY_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4937454.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11270..12145
                     /locus_tag="ACG9CY_000926"
     CDS             11270..12145
                     /locus_tag="ACG9CY_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4937455.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12297..13139)
                     /locus_tag="ACG9CY_000927"
     CDS             complement(12297..13139)
                     /locus_tag="ACG9CY_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4937456.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13231..15108)
                     /locus_tag="ACG9CY_000928"
     CDS             complement(13231..15108)
                     /locus_tag="ACG9CY_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4937457.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15105..16526)
                     /locus_tag="ACG9CY_000929"
     CDS             complement(15105..16526)
                     /locus_tag="ACG9CY_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4937458.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16529..19918)
                     /locus_tag="ACG9CY_000930"
     CDS             complement(16529..19918)
                     /locus_tag="ACG9CY_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4937459.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20175..21566
                     /locus_tag="ACG9CY_000931"
     CDS             20175..21566
                     /locus_tag="ACG9CY_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4937460.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21639..22286)
                     /locus_tag="ACG9CY_000932"
     CDS             complement(21639..22286)
                     /locus_tag="ACG9CY_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4937461.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22294..22980)
                     /locus_tag="ACG9CY_000933"
     CDS             complement(22294..22980)
                     /locus_tag="ACG9CY_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937462.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22986..24059)
                     /locus_tag="ACG9CY_000934"
     CDS             complement(22986..24059)
                     /locus_tag="ACG9CY_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4937463.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24281..24955
                     /locus_tag="ACG9CY_000935"
     CDS             24281..24955
                     /locus_tag="ACG9CY_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4937464.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24960..26300
                     /locus_tag="ACG9CY_000936"
     CDS             24960..26300
                     /locus_tag="ACG9CY_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4937465.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26449..27435)
                     /locus_tag="ACG9CY_000937"
     CDS             complement(26449..27435)
                     /locus_tag="ACG9CY_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS4937466.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         7996 a         5208 c         4999 g         9269 t
ORIGIN      
        1 agttcatatt cattgtttct tataatacag tgttcaatac ttctcttaaa gcacttgttg
       61 atttgctgaa cattgctttt tcttcttcgt tgattgataa ttcaacaact tcacgaacac
      121 cattttcgtc aacgattgat ggcacacctg taaagatatc tttttcgtcg tattcaccag
      181 ttaaataagc agatacaggc aatacagctt gttcgttatt caagatagct tttacgatac
      241 gtgcagtact cataccaata ccatagtaag ttgctttttt acgatcaatg atctcataag
      301 cagcattacg gactttatca gcaatcacat ctaattcatc ttgggcaata cgatggtctt
      361 tttctacaat ttcaaacact ggtttaccac cgacagttgt atgtgaccat gcagcaactt
      421 ctgaatcacc atgttcacct aagatatagc cgtggacgct gcgtgggtca acttttaatt
      481 tcaatgcgat ttctttacgg aaacgagttg tatccaatgt tgttccagta ccgataacac
      541 gtgatgttgg cagaccagat tcgttccaag cgatatatgt caaaatatct actgggtttg
      601 aagcaacaac gataataccg tcgaatcctg atcccatgat ttcttttacg atttggcgca
      661 tgattgacgc atttgtttta actaaatcta aacgagattg accaggtttt tggttgatac
      721 cagctgtgat aacaacgata tcagcatctt tacattcttc atagccacct gaccatacag
      781 ccacattttc atcgccccaa gccataccat ctaaaaggtc caatgcttcc ccttctgctt
      841 tttcttgatt cacatcgatc aatactaatt cattagagat tccttggttg atcattgcat
      901 aagcgatact tgttccaaca aaacctgtac cgacgattac tactttacga cttgtttttt
      961 tcatgaataa acagctcctt ttcataacta tttgttactt tcatgaaaag tatagtcttg
     1021 tttattcatt ttatcaacat gttaattata atagttgtta ttataatttt tgatggaaac
     1081 gaattcctga atacgcataa cagataaatg acaaatgttt acacctatat cccctgataa
     1141 tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa ttcgctgcaa cgatataata
     1201 agacagcaat cgcacctaaa acaattttcc ttcatatgct ttagtgaaag attttcttct
     1261 taatgtttat ttctccattc attgggagta catgttgaat acttcttgaa taatttgaat
     1321 aaagaagtcg tttgtttata tccgatacga ttagcgattt cttccaacga aaaactcgtt
     1381 tcttgaatca gttccaacgc aaagtcgaat tttttcttta gaaccagttc ttgaaaggaa
     1441 gcatcaaaat acattttcaa tttattacta agatagaccg gattgtagcc aaaatgagct
     1501 gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga tataatctaa tactctaagc
     1561 atctcatttt ctgcgcatcc atctgctgat gaaaagcttt gttgttctaa taaatgagtc
     1621 aattcgatca gtaatagtga tgtatagaga tttaacgaac ggatcttatg actgtatgta
     1681 tcaaaataaa tcaacagcat attttcaatc aaatgcgtca aacgacttgt ttttccgccg
     1741 cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg atgcatttat caaaaaagaa
     1801 gaaagtagat tatttgtgct tgccaaatga ttcaatatct ctgtagataa ggtttcttct
     1861 tttaataaga tgttgattaa aatatcctct tgccctgtgt attcgattct ttgagtagct
     1921 tctttatcaa tgatcaccac gtcactttct tccatctcta tcctttctcc attaataatt
     1981 tgtaaacatg taccgctata catataattg atttcaacaa aatcatgtgt atgctcggga
     2041 acttccgcat atcgacggtg tttactgata tgaatatttc cctgttgaaa tagatcccct
     2101 cggtggattt ttggcggtgt ctgaagatgg taagctaagt ttaaatcatt gatattcatt
     2161 ccttgttcac gctgcagagt ttcgattgat tcttcgttac gcaaaaaaaa tgtctaattc
     2221 agctagttcc aattgactcc ctcctttttg taagcggttt ctttcgctta ttagttcacg
     2281 ctgtactgct attatatgca aagctatttt tggtttcata aagactttct atggttggga
     2341 catgaacttc ctgtgattcc gctattgaga gaataaccac ttcatactat atttttgata
     2401 agcgcttaca aaaatgaaag gaacatgctt atgaataaaa agatgatgaa attttctctg
     2461 ctgtcagtct ctcttatttt gacttctgct ggtgcagtca cgtctgcaat tcccagtatg
     2521 tcagctactt atgccacgcg ttctttatct gctgttgaat tattaacgac tgctccctct
     2581 tttatggtca ctatttttgt tttgattagt agttttattg ctaggaaaat cggacaaaaa
     2641 caaactgtga tcatcggatt agtgattgca tcgatttgtg gtatcctccc cctcttctca
     2701 caaagttaca gtattgtctt agcttcaaga gctggactag gtattggctt tggattgatc
     2761 aattctttgg cagtcagcat gattgctgcc ttttatcacg gagatgaacg agcctctatg
     2821 attggttttc aaagtgcatt tcaaggattt ggtgcagctt taatgacatt tgttgcagga
     2881 cagctagtac agttcggttg gcaatatacc tttttagtct acgccatcag cttacctatt
     2941 cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca atcgcatggc
     3001 acagccccaa caagacaagt aatcaaaacg aatcctaaaa tgattagtta tggtattttt
     3061 ctcttgctgg tcatcgtggt atataatgct gtatcgatta aattagccac agtcctgacg
     3121 acttattctc taggaaacga aactaatgca gcgaatatct taacaatcat gcaattggca
     3181 agtatggctg ccggagtttg ttttggctgg cttcagacaa aagcacatcg ctatatgctt
     3241 acactttctt tactactgat gggaatcggc tttgtattga ttgccactgt gataaacctg
     3301 tatgtgattg ggtttggtgc gatcctaaca ggaatttctt tttcgttatt cattccttac
     3361 ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg agagtttcaa tacttcaatc
     3421 ttattggttg gcgctaattt aggtactttc attgcaccat atggtttgct ctttctctct
     3481 tttgcttctc ttgggagtaa aacacttgct gttttcgtca atggcagtat cttgctgttg
     3541 attagcgcgc ttattagcct tctagtagta cacaggacga atagaaagac agtgataaga
     3601 gtagatgaag aaataaaaga acaattgaca acaaaataaa tttgatggag gtcgtgtaaa
     3661 atgacaaatg aaaaacataa agatagatgg ctttggtatc ctggagactt tgagatccgc
     3721 catggactgc tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc agcgtactgg
     3781 tacatggatg attgccatcg gaacgtcaag tttaagagat attactttct tgatcaacct
     3841 tccatgttta aagtgacgat ccaaggagtt gggtatgtag aaattaatgg acaaaaacat
     3901 ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa aaattcgtat ctttgtagga
     3961 catacatcag ggctaccagc gatgtttatc gagggagacg aagtaaaatc agatattggc
     4021 tggacagcaa gtaattttat tgaagaatat ccagctggct ggagtccgct ttatgtcgat
     4081 atcgcaaaag atccaaatca aatctattat caaaaagaat gcgtccatcc agtcactgaa
     4141 acggagattg gcggaggaat cttattcgat tttgggcgag cagtgaatgg ctgtgtagaa
     4201 ctaatatctc tggatgacca tatcgaatct atcactctat gttatggaga gtccgatgta
     4261 gaagctatgg atatcgatta ttgctactat aagcaaatag gaatgacaag aaaaatgaaa
     4321 gctcgtaaac gcgcttttcg ctatttgttt ataccagagg tttctactgg aatggttcaa
     4381 cttcaagttt ttcatgaatt tttatctctt tcgaaacaag ctgctttttc aagcgattct
     4441 tctttactga atcagatctg gtcagtatca gaagaagcct tcaagttatg tagtgatttg
     4501 ttttttatcg acggtatcaa acgagatcgc tggatttggt ctggagatgc gtatcaatca
     4561 ttcatgatca atcaatatct tttttttgac gaggaaatca ataaacgaac gatattagca
     4621 ttaagaggac aaaatgaaat caagcagcat atgaatacta tcgttgacta ttcgatcttg
     4681 tggttgattg gtatcgaaaa ccactatatg atgacgaaag actatgaatt tttacgcatc
     4741 gtctacccta agatgcaatc attaatgaga tacttgatgg agcaaacgaa tgaattaggg
     4801 tttatttatg ggagagcaaa agattggata tttattgatt ggtctgaaat ggataaagaa
     4861 ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct atcattccat gatcacttgc
     4921 ggagatatct tagctgaaac atatggagag aacgaaaatt atcatactca tctctatcaa
     4981 gaaaaatatc aactattgaa acaaaaccta tttgactact tttgggatga agaacttggc
     5041 gcttttatcg attgctatga atctgggaga agaaatgtga cgcgtcatgc gaatattttc
     5101 gctgttttat tcgattttgc taacaaagac caacagcaat cgatcttaaa gaatgtctta
     5161 ttgaatgaca ctatcacaca gattactact ccatatttca aattctatga acaagatgct
     5221 ttatgcaaac tcggacagac gaatatcgtc taccaaacga ttcttgatta ttggggaggg
     5281 atgttatctc atgatgcggt aactttctgg gaagaatacg accctactca aacaggaaat
     5341 gaaaagtaca gtatgtatgg agatccttat ggaaaaagct tatgccatgc atggggagca
     5401 agcccaatct atcttttagg acgctatttt atcggtttgt atccaacaaa gccaggttat
     5461 gaagaattta tgatacaacc taatctagat tcatttgaaa cactgaattg tacgctacct
     5521 atcaaagatg gaaagatttc gcttgaaaaa acaaaagata agatcgtgat tacttctgat
     5581 agaacaggcg gaacagtgaa aatgaatggt aaaagttatc ctcttctagc aaaacagcca
     5641 cttgtcttaa caagctattc aaaaataaat gcataaaaca aaaaactgct tgcgacatat
     5701 cagaagactt taaatcttct tctgatattg tccaagcaat ttttattttg aagtttgctt
     5761 aattctgttc tttagaaaca atgtttgttc tttttccggc aaaaagaaag ctagataata
     5821 cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg tattctccta
     5881 gtttcccaag tacaagtcct ggtactagat aatcagtatc ggcaaatgta gaagaagcga
     5941 ttttaagatc tcctaatact ggaaggataa ataatggcag ccaactaatc aataagctat
     6001 tgaaagcaga acctaatacc gcccctcttc taccgccagt cgagttgccg aagacaccag
     6061 aagcagctcc gcagaagaaa tgtcctacta ccccaggaat gataactgta gtttgcaagc
     6121 ctatcatagc aaacatggat agtgttccta caaagaagct aacaaaaaag ccaattaata
     6181 cagcattagg tgcatatgga aaaacgatag gaacatctaa tgctggttta gaatcgggta
     6241 cgagtttttt agcaatccct tggaatgctg gaacgatttc tgccaacacc attcgaaccc
     6301 cttgaaggac gataacaaac ccagctgtga atgttccagc ttggatcagt gcaaagatta
     6361 cagcgttgtc tccattgctt agttctcttt caacaaatcc aggaccagct aacaaagcaa
     6421 gtgcgacata tacgatcgac atcaagagca tgatcgatac agtagaatct cgcaaaaagc
     6481 tgaaactttt aggaatcttt atgtcctctg tagaacggtc tttgtttcca aaaaactttc
     6541 cgatccatcc actagaagca taaccgatat ttccggtatg ccctaacgca acttgattgt
     6601 ttccagtgat gttcttcata aacggttgac aaagagcagg cgtaatagtt aatagtgttc
     6661 cttcgaagac cccgcctaat aagatcatcc acaaatttgc agtaaacccc gcgcttacta
     6721 aaatgactgc tgttagacaa gaaacataaa gcattgcttg acctgtaaga aaaatgaatt
     6781 tatatcttgt gaatctagca agtaaaatat tcacgatcat ccctactagc atgatcaaag
     6841 cagtaggtgt accgtattgt tgcaaagcaa tcgccactac tgcttcattg ctaggaacta
     6901 ctccttgagt atgcagagct gtttggaaca tcgaagcaaa aggggttagt gaattactga
     6961 ttaaaccagc accgccgatc agtactagga atccgacaaa tgttttgata gttccttgga
     7021 tcacttctga aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc aaagaaacga
     7081 ggatggaggg ctgactgacg attgacacaa acaaattcaa caatttatcc attgatagtt
     7141 cctcccttga atcattttta ttcttttgac aagatcttcg ttactttttc ttttacttct
     7201 tcgctgtcaa tgatcgattg caaaggtacg agcttttcat caggtataga agttagcgta
     7261 tctttcaaag aatgctcaat gaagaaataa tcagccatat cgctagatac acttccaaca
     7321 tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag tgcttgtttg
     7381 atgttcatct ctaccataaa acttgttcct aatccacttt gacatactgc aaaaattttc
     7441 atatcattcg tctccttttc ttatgatcgc taatatttct tcttttgttg atgcgtgtaa
     7501 taaatcttga agattttgtt tattagaaag tagctttgtt aaatttgcca aagcccgaag
     7561 atgtgcctca ttatcgatag cagctaaaca gatgaacaaa ctaatgggat gggctggatt
     7621 atcaagcaga tccacaggtt gttgaagttt cagcaaagac atccctactt cgtttacgcc
     7681 atcttctgga cgagcatgag gtaatgcgat gtgatcgcca atatggataa acgcaccata
     7741 ctgctccacg cgctcgatca tagctttgat ataactttct tctattttat tttctgataa
     7801 tagcggctgt gcggcaaatg tgattgcttc ttgccagttt tttggttgat ccgataattg
     7861 gatcatttct tctgttaata attcagatag cataggacgt tgatcctcct tcttttctaa
     7921 aagtttgttc attttacgat ttaatacttg atagagcttt tcttcgtcta cgccttcttt
     7981 taattcgata taaggcagta gcgccgacaa caattcttgg gcagaaggca ttgaaaatcc
     8041 tggtatcagc caatctcttt gtacttgatt gatcagctga tttttttcca agtcagtcat
     8101 gattggatgc agaacataga gagacttttt cttatcagta agtgtgagag gtacagtaga
     8161 aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg aattagtctc
     8221 tgtaaaaaag atcatcggga ataattgctg aagttcactt tttaagatca atgaagagct
     8281 aatgccactt ggacagacga tcacggctct tatttttcta tttctttctt ctgcgtccgc
     8341 ttttctgatc tctccgccaa atagtatagt gaaaaaacca atctcttctt cagggatgct
     8401 cttccctatt cgtctttcta acggaagtaa tgcttttctt gtcatttcgt agatggaagc
     8461 atattgatgc ttaatttgat cgatcatcac attaggtaaa taaaaagaat aggaaatacg
     8521 aaaataggca gggaccaagt gataaaagac attcatcaat aattcacgaa acgaagcaaa
     8581 ttctacggcc atcaggaatt ccatgcgatg gatgacttct gaagaacatt ccagcaaaaa
     8641 atcaagtgca ggctcctgga catctccttc aacgatcgat gctaagcaaa gagagaaaaa
     8701 tataatctcc gaattttgat gttcggaata gccaaggcca gtcaatactt gtcgactaat
     8761 gtcagtgact gcaagagaag ccaacagttc agcatctttt accagttcca tggatgtttt
     8821 tccaattcga atggttgtca aactcaaata ataagctaat tcatccattc gatttggtgc
     8881 gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa tctctaactt tggcgtaata
     8941 atcaatggaa agggttgcgg ttaaatacgt gagcccaaat cttccagcaa gtgactgcat
     9001 caaacgagca gtgcgatttt ttgcttctct tcttaatatt gcttctgacc ctagcagcct
     9061 aaaaccagtt ttcctagtat acgacagctg acaatcttcc acttctaatt catttcttat
     9121 ctttttgata tctgacagga ctgtcccttt agaaacctga agaaaatctt gaaaatgaaa
     9181 aacagaaaca gacgttgcag tcgaataaac catcaaataa agaagatctc ttcgctcttg
     9241 ttcggtatac acagcttcaa atcgagtaat atccaaaaat ttttctgccc agtcgattgc
     9301 tcggaatttc tttatacgga tacgggtatc atccacatat tcggcaaaag gagttaattg
     9361 tttgatcgcc tcgcttgtat ttacttcgat gatctctgct gctttttcta gactgatttc
     9421 tcttggtgtt ttcaggagat aactaatttt gtagttcaca gcgctgttca cctcctactt
     9481 atattctagt atggtagcgc ttttaaatcg aggggtgttt ggtacaactt acagttggat
     9541 tttttttacg actgtaccga acgatttcta tcgaatggat acagaaaaaa agcaggtgac
     9601 caaatgtctc ctgcttttat gaattgattt cgtccgatca atgatgatca tgtttatttt
     9661 ttgaggtcag ttcttcaaat ttttctttga catcttcaac cgctttttcg atatcttttt
     9721 caaagtgatt gggctcatta ggctttgttt cctcttctac agaatttgaa ccagataaag
     9781 ctgtttcttt attttctgtt tcaccagagt ttgtgccagc taatgattct tctttgtctt
     9841 ctgtttcacc agaattagtt cctgctaaag ccacttcttc ttctgtttta ggggccattg
     9901 aacctcttaa tcgtttgtgt ttttcaccgt cataatttgt catgttggtt cctcctagta
     9961 gttctatact ttttcatcgt acaataagac agtttatgcg tcaaaaaaca aacccgattc
    10021 ttacaaatat gtcatatttt tccttctgat tcttgaatgc ttttgattcc ttcttccaaa
    10081 attttcattt gtaagattgt ttcttcatct ttgatgactt tttctgcgcc attaactaat
    10141 gtctcgtaaa tcccttcgta gacacggctg taatcaccaa cctcggaaat tactttttct
    10201 tcatgataac tcccttgttc atctatatag gtcaatacac cataatgttc gggcaaatcg
    10261 ataccaaagt cttcatgatc aggcatgtaa aacattttca gatgttcttc ttgtcgatct
    10321 tttgtttgtt tcacgaacat cccttttttt ccgtaaacga cgaaacttgg acgttctttg
    10381 atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa atccagatcg
    10441 aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct gatatcatct
    10501 ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc atataaatag
    10561 ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa ataatcataa
    10621 tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt ttggactgtc
    10681 aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc tttttctttt
    10741 gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt ctctactagt
    10801 acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg actaggtgta
    10861 gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg atcagtatat
    10921 tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt tttgatattg
    10981 attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact ctttccattg
    11041 ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt tcagtatatc
    11101 ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc agaaaaacac
    11161 ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat caaaaagata
    11221 gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca tgcttattga
    11281 agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg tcgattacat
    11341 ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa aagctacgta
    11401 tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg gatttaaaga
    11461 attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca atcaaacgat
    11521 tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa agaaaatggc
    11581 ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg aagatttaga
    11641 tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa aaggtgattc
    11701 tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt ttttgatcat
    11761 ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg aggattgcgc
    11821 catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga cctatttgaa
    11881 gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa tggccaaaca
    11941 ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag tagcgacttt
    12001 ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga tatattctca
    12061 aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc aaaaaggcct
    12121 tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag aaagatagtg
    12181 tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt tttcaaacct
    12241 cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag gaatagctaa
    12301 cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag gaaaatcagt
    12361 atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac tttctgtact
    12421 tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag taagcgatga
    12481 ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc tttttcgatt
    12541 ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg ataagcgagt
    12601 gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg taagtctgtg
    12661 aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt attttctccc
    12721 cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag cacagtattc
    12781 gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat accgatcgta
    12841 tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt aaagggatct
    12901 gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat gttttcttgg
    12961 attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg agagatgatc
    13021 tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag tatccctatt
    13081 cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt tggtttcatt
    13141 tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt gcgtaatccg
    13201 ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc gtctagcaaa
    13261 gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat agattccctt
    13321 accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag gagaaatcaa
    13381 ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt ttgttaatgg
    13441 aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt ttagacctgt
    13501 cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt tcgccgatac
    13561 ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt cactatcttg
    13621 atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt caccatctac
    13681 tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt gatcatttgt
    13741 atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg ctttttcgtt
    13801 caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag gaatataatt
    13861 cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat ttactgggtc
    13921 gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat cattatcaaa
    13981 tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt tttgcgttcc
    14041 ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa ttttttcacc
    14101 gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag acgtatcatt
    14161 tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag gcgtgattgt
    14221 ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat tatattttcc
    14281 aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg caaagtcatt
    14341 gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt gatcagttgt
    14401 ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg gtgttccttc
    14461 ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag taccgatttt
    14521 tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat ggatagtatc
    14581 tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct catatacagg
    14641 gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt ctttgatcgt
    14701 gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat ttccatcagc
    14761 atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg cttgttttgc
    14821 tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga cgttataaac
    14881 tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt ctttcccgct
    14941 gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga tgacttgagc
    15001 tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta atggtaaaat
    15061 aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt tttcactcct
    15121 gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc aacaacgcct
    15181 aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt ttctttttta
    15241 ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt tgaaatggta
    15301 aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc ttttaatcga
    15361 taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga aatcaggtga
    15421 agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg gaggtaattt
    15481 cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg tttatttgtc
    15541 tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt actgacacca
    15601 aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc tgtactgctt
    15661 agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa cgaagggatc
    15721 gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc tgtcagcttt
    15781 tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc atccgcacta
    15841 tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt atgcaaggaa
    15901 taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac cttctcaaaa
    15961 ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt tgaaagtttt
    16021 tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt ttctttgacg
    16081 acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc ttgatcttta
    16141 gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt tgttgcttct
    16201 gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc ttcttctacc
    16261 gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac ttggaatgtg
    16321 acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc atttggatga
    16381 tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg aaccatttct
    16441 gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa gaagagacac
    16501 atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt ttcaaaaaaa
    16561 gataccaaat agaaaacgca caaccaatca tccctactag atagattcct aaacggccaa
    16621 ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa gaaatctggt
    16681 tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact tgaattgacc
    16741 catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct tctggtgtaa
    16801 aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag gtatctgttt
    16861 cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg aattttgctc
    16921 cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc
    16981 gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt cccgtgtaaa
    17041 tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta tcatatccgg
    17101 caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca aaactggcaa
    17161 gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt gcttcttttt
    17221 tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct tgcatcgcat
    17281 acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac tggatcgtat
    17341 catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa gcgatcttga
    17401 tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt tctgtcagcg
    17461 tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt ccgtcgccat
    17521 gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg ccaataagat
    17581 ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg aattgtttag
    17641 tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca ggtaattctt
    17701 tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt gataatatct
    17761 cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt tcccacgtat
    17821 ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa gctgcagcat
    17881 ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca gctagattgt
    17941 tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct ttagcagatg
    18001 gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt gtcctgccgt
    18061 tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt ctcacttggt
    18121 aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg atggtatttc
    18181 cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc ttgactgaaa
    18241 gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt ccattgctta
    18301 cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct gttgcatgat
    18361 cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt cgcatacgag
    18421 actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg atgccaagac
    18481 catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt gcgataaacg
    18541 tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt cttgagatta
    18601 gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa taattgctgc
    18661 ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca tccgtcagca
    18721 aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca tctcttagtg
    18781 ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt acttgatttt
    18841 tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat ccttcgcttg
    18901 agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct aaagtatcga
    18961 caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc atacttcctg
    19021 accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca tttccacgag
    19081 cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac tttcgtatcg
    19141 catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc cgatcattag
    19201 gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta ttgccttgat
    19261 gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa gtgccctgat
    19321 catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca gctggcaagg
    19381 ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat gattcttctt
    19441 ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct gtctcatcaa
    19501 taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg
    19561 gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa
    19621 ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc acatcactta
    19681 agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg ttattcgcat
    19741 ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca gctgaaattg
    19801 tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc acaaagtttg
    19861 ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg gtcgtcattt
    19921 ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat taaaataaac
    19981 aggcgttaca taattttatt tattttaata tgataattat tatacgaagg gcgctaaagg
    20041 gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc ttcgattaat
    20101 gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg ttgttggaat
    20161 taaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac aagtggatga
    20221 ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta aggtatatcg
    20281 aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa aaattggggt
    20341 atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa atgtcgcaaa
    20401 gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa acgattgagt
    20461 attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg aaacaatgca
    20521 atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt cagttaacta
    20581 gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta agttactacg
    20641 gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt atggtcaaga
    20701 ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct ttctttatta
    20761 tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa tggaaagctg
    20821 gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc ttgaaaaatc
    20881 tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt gtcaatacga
    20941 ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata cgaaaaaata
    21001 tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa tatgttgttc
    21061 aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg actcatttga
    21121 cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt ctatatgctg
    21181 gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg atttattatt
    21241 tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat tatacttctg
    21301 ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat attgaagaat
    21361 atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct attactgaag
    21421 atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat caacccattt
    21481 tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca aatgaagaag
    21541 aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata aaccaccctg
    21601 ataaaaattt caggacaaat acacatagct gtttcttttt attgtattcc ttcagcaatc
    21661 caacgagatt tatagcgaaa gtcttttaac aactcttctt ttgtccaatt atttgcaatg
    21721 agatccgtca aagtgttcat tacgagggtc atggaaaagt ataggaaact aactgcttct
    21781 tccgacgatt gaatggagaa atgattgatt cttaataatt ccagccattt ttcgttcatt
    21841 ggagaatcag ccgatagttc tctacgtttg gttgtctgct gatcatttcg ctgggttaac
    21901 aagtataacc tttgccaata gatactttcg tgatctaagt cgcagcacca ctctaaatat
    21961 ttttcgatcc ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa gatatcttga
    22021 tggactatgt gagaactgct tttgatcgta tacaaataag catcttccaa atcttgaaaa
    22081 tatttataaa aagcaccgcg cgacatcccc atcttgtcaa cgatttctga gactttgact
    22141 tggctgatgt gctgtgagca aaatatctcc agtaataggt catcgattct ctgtttcttt
    22201 gcgttcgata aattgaaaaa agtttctttt ggcatccaca tctctccttt atctctgatt
    22261 atacgtggat agcccttcat tgtcatgaag gattcattcc ttctttttct cttcttctgt
    22321 caagtaccca tcttccattc gataaacgcg atcactccat tgaatcatac ggacatcatg
    22381 agtaaccata atagtcgctt tttgtttctc atgagcttct ttaactagta atttgacgac
    22441 ttcatatgca tgatctgtat ccaaactagc tgttggttcg tctgctaaga tcaagcttgg
    22501 ctcattatat aaagcgcgag caattgccac tctctgtctt tctccaccag aaaggtcttt
    22561 aggataacta tttttaagtg actggatatc taaagattgc agcaaggaat cgatttttgt
    22621 tgaatcttct ttttttgaag taacttggtc aactagttca aattgttcct ttaccgttaa
    22681 aaaaggaatc aaattagagc tttggagaat aaatcctatt tctttaaagc gtaaatctgc
    22741 tcgttttttt tctggaagct cagttagtga gtgaccgttg atcactattt ctccggaagt
    22801 cggtgtttgc aatcctcctg aaatggtaag gaaagtactt ttacctgaac cagaaggacc
    22861 aatgatgctg acgaattctc cttcctttac ataaaaatca attgctttca aagctgagac
    22921 ctttgtatga ccagaaccaa atgttttggt aacttttttc atcgttaata tttttttcat
    22981 ttttattaac ctcctattgc tgtaattggg tctactttgg ttatcgtacg tatcgaaaac
    23041 aatccgccta gcattgcgac aatgatcaaa atcccactat atagcaacca ttgtgaccaa
    23101 aaaacagcaa aaggcatcgc gcttggtaaa atcagactag tcaaataagc aaacaaaaga
    23161 gcaagcagaa cgcctaccac cccaacaata aaggactgtg ctactaaaga tttagcgatg
    23221 aagaaatttt tgatcccttg tgctttcatt actccaaaaa tagcagtctt ctgaagtgtc
    23281 atcacgtaca tgaaaatacc gaccacggca gcaaccacta agaacaaaaa gtatatcatc
    23341 gcatttaagg tcatattttg ggcagaatag ccaggaatac tttcaatgaa tgtcccaata
    23401 gttaataact gtagaccctt cgatgctttt tctttcgaaa ttttagctga atcctttatt
    23461 acgatcccat tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc atacttcaaa
    23521 gcagtccacg tatctaggga cgcatagatg acgggagaaa ctgtgtaata agtttcagga
    23581 aagataccga caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt atctccaatt
    23641 ttatagcctg aatcagccag attttgagag atgatgattt cattctttgt tttaaattca
    23701 tgccctttcg tgagttttgg taataaaaat gcttctttcg ttgtgccgaa aactgtaaca
    23761 ttttctttgt tttttccttt gattgctccg ctgtacaatc cgattggtgc tttctctcct
    23821 ccttcaatgt atttcagatc tcctcttgtt aattgtgaag ctgcaaatac ttgatttgca
    23881 tcctccgaca aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt aaactcttct
    23941 gaaagtccat tcgctagtcc tgacaacatg aatacgacat acgcaatcaa gaacataata
    24001 ccgacgatca agccatatcg taatttagaa tagcgaattt ctttgatagc tagaaacatt
    24061 tgtttttcct ccctatgata gattgatagt gacaaaatgt cacttgttga atagtgtaac
    24121 tctaaagtga caatttgtca cttgtgttgt ttttttctta tcaataaatt gtctaagaca
    24181 tctttcgata aaaccttaac atgagtcata aaaaatttat ttaagcctta tttatgcttt
    24241 ccatgataaa ctaaagtgac caaatcaatg aggtgggaaa atgattaaac tattagtagt
    24301 agaagacgat aaagtattat ccaataatat aaaagaaata ctcgctgcaa tcggcgagat
    24361 aacacaagtt tatgatggta gtgaaggact gttcgagttg gaaagtggag tttatgatct
    24421 tgcaatcttg gatttgatga tgccggaaat gaatggatat gaagtgttag caaaaatacg
    24481 gaaagaaaaa atacagactc cagtattgat tttgactgca aaagatagtt tggaagataa
    24541 gattgaagga tttgaacgag gtgcagatga ttatctaaca aaacctttct atcgagaaga
    24601 actgatcatg cgcgtgaaag cattattgaa acgctcgcta ggtctctttg atgaaaatgt
    24661 tctgaagtat aaggatatca gttgtgattt aaaaagcaat gaagtcttgt ataaagaaca
    24721 agttttgccc atacaaggaa aagagtttga attgctcgtt tattttttac aaaataaggg
    24781 aatcattttg acaaaagagc agatatttga ccggatctgg ggatttgatt ctgatacgac
    24841 gattacagtt attgaagttt atatgagcaa cttacgaaaa catctgcgaa gtactggatt
    24901 ggataaagaa atcaaaacct tacgtaatgt cggttatata ctgcaaaggg tgtgatgcaa
    24961 tgaatggaga tattacacga aagcaacgta gtcgcttttt tgcgacaaat gtagcagcat
    25021 ttgcttttat cttttttctt ttgggattca ttactttaca gatattaaac caatctgcat
    25081 acagagaaac agatctgtca ttagagaaaa taaattttga ttctcgaaca gtacaattag
    25141 agatacaaag atatcagcaa ggcgatccct ttttaaaccc gactcaaacg gaacaaaaca
    25201 gaacaaacat ggaacagcca ttaatggaac catcaaatga ccgttttaac actcaagtga
    25261 tcttgtggtc taaaaatggt gagatattga ataaagcagc tattggtggg cgtttcaacc
    25321 aaatcaaaga attaaaaatg gatacgaaaa atctgaatac catccaaaat attgaattaa
    25381 atgaagacag tggctcatct gccctctctt tccgttcgat tacgaaaaaa gcggagacga
    25441 atcaagatgg cgttgcctac atccaaattt tagaaaatac aaatcaaatc aagaattcat
    25501 tagcgacttt tcgcacgatt atgattttgt gtatgattgt tttttggatt ctctctattg
    25561 gtatcagtta ttacttgtct agtctaagca tgcgccctat tattctttct tggagaagac
    25621 aaaaagaatt tgttgagaat gcttctcatg aattgcgcac accgttaacg attattcaaa
    25681 atagtttgga acatcttttt accaagccta atcatactat tattgaagaa tccgaaagca
    25741 tcgctcaagc attgtcagag acgaggcgcc tcactggatt gacttcggat ttgctgacaa
    25801 tcgctcggaa tgattctgat cagcagctgc tttcaaagca aatgatcaat acacaagaat
    25861 atatcgaaca acttgttaaa ccatttcaag agatggccat catagatgga aaagagttta
    25921 ttctccataa cgatgcaaca ttagaagtac caattgatga aaaaaaaatc catcaagttt
    25981 tggttatttt gctggataat gcattaaaat atacgaatcc agaagataag atcattttgg
    26041 attcatctgt taaccataaa gaatggctga ttcacgtaaa gaatactggc ccttctatca
    26101 gtgaagaaga tcaagcaagg atttttgatc gattttatcg ggaagatcaa tctcgggcga
    26161 aagagactgg cggatacggg cttggcttgg caattgcaaa acaggtaatc gaacagcatc
    26221 atggacagat ttctgtatcc aattatcttc cacaaggagt cgattttaaa gtccaattgc
    26281 cgttgaagcc taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa aatcttgtca
    26341 cagccctttt ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg aaataagccg
    26401 aaatttcttt aagctgacca cttctgtcac aacctcttta gcttacgctt acgatttttt
    26461 attgcggtct tcaatgtgta catcaccgct tgtgtcaata tccaattctt ctcggctaac
    26521 atcttctgtc actttttcaa catcttcatg ttcttcttta tgaatcccaa cttcttcacg
    26581 aataactgta tgtttgctta catctacttg ctcttctttg attgggatag taatcgtgtc
    26641 atcttccaat ttttcgtctg ttccctgtga agattgatct gttactggtt tgcgttcgat
    26701 gacgatttct tcacgtttga ccggtacttc taccgtttca gtatcgttga ccacatgttt
    26761 gtggatgtcg acttctccag ttgtcacatc atgtgtattt acatctaatt tttcttcttt
    26821 tagttgaatt gtatcatcat ctagatttgg tttgcgatcg atgtcttcag tcttgcttgt
    26881 acccattgta tcagtctcgc caggtacgtc catcggatca gttcctggtg tcatattcaa
    26941 tggatccgtt gtattttgaa agcctgtcgt gccacctacg tccattggat ctgttccagt
    27001 gccagtttct aatccagtcc ccattgtgct tgctgggcta acagtatctt gtgtatccat
    27061 tgtatcttct gttgtttctg gctgacgata gccttttact gtgatgacaa attttccatc
    27121 agaaatatct cctctatatt gatataaagg atcattttct ggtgtagccg tctcagaatc
    27181 ataggtatca aaagaaaaag tatcttttat tttttcccat aaagaacggt cttcttcacc
    27241 atccatccga ttttcatctg tttcaacatc tataccagac aatccgtcaa atcctagtct
    27301 agatgctgct tcatcagtag tataaataac gatatcatct cgttcatacc cttcgttacg
    27361 caaccgttca attactgata atgcttcttc cctcgtacta tagcttccga tgaccgttaa
    27421 atttttctct tccatgtaaa ttcctcctag caaatagatt tatgattggt at
//
LOCUS       DAZULG010000023        26112 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000023 DAZULG010000000
VERSION     DAZULG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.023"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..272
                     /locus_tag="ACG9CY_000938"
     CDS             <1..272
                     /locus_tag="ACG9CY_000938"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="catalyzes the conversion of dihydroorotate to
                     orotate in the pyrimidine biosynthesis pathway; subclass
                     1A is a dimer formed by two identical PyrD subunits each
                     containing an FMN group; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS4937467.1"
                     /translation="IKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASM
                     LQIGTELHKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            407..1858
                     /locus_tag="ACG9CY_000939"
     CDS             407..1858
                     /locus_tag="ACG9CY_000939"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS4937468.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            1879..2715
                     /locus_tag="ACG9CY_000940"
     CDS             1879..2715
                     /locus_tag="ACG9CY_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4937469.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            2715..3374
                     /locus_tag="ACG9CY_000941"
     CDS             2715..3374
                     /locus_tag="ACG9CY_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4937470.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            3500..4189
                     /locus_tag="ACG9CY_000942"
     CDS             3500..4189
                     /locus_tag="ACG9CY_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937471.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            4195..5151
                     /locus_tag="ACG9CY_000943"
     CDS             4195..5151
                     /locus_tag="ACG9CY_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS4937472.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            5195..5707
                     /locus_tag="ACG9CY_000944"
     CDS             5195..5707
                     /locus_tag="ACG9CY_000944"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4937473.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(5963..6511)
                     /locus_tag="ACG9CY_000945"
     CDS             complement(5963..6511)
                     /locus_tag="ACG9CY_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4937474.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(6600..7781)
                     /locus_tag="ACG9CY_000946"
     CDS             complement(6600..7781)
                     /locus_tag="ACG9CY_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS4937475.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(8017..9525)
                     /locus_tag="ACG9CY_000947"
     CDS             complement(8017..9525)
                     /locus_tag="ACG9CY_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS4937476.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            9684..9875
                     /locus_tag="ACG9CY_000948"
     CDS             9684..9875
                     /locus_tag="ACG9CY_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937477.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            10078..10995
                     /locus_tag="ACG9CY_000949"
     CDS             10078..10995
                     /locus_tag="ACG9CY_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS4937478.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            10988..12061
                     /locus_tag="ACG9CY_000950"
     CDS             10988..12061
                     /locus_tag="ACG9CY_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937479.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            12209..12727
                     /locus_tag="ACG9CY_000951"
     CDS             12209..12727
                     /locus_tag="ACG9CY_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937480.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            12708..13100
                     /locus_tag="ACG9CY_000952"
     CDS             12708..13100
                     /locus_tag="ACG9CY_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS4937481.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            13176..14111
                     /locus_tag="ACG9CY_000953"
     CDS             13176..14111
                     /locus_tag="ACG9CY_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4937482.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            14244..15023
                     /locus_tag="ACG9CY_000954"
     CDS             14244..15023
                     /locus_tag="ACG9CY_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4937483.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            15016..15333
                     /locus_tag="ACG9CY_000955"
     CDS             15016..15333
                     /locus_tag="ACG9CY_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4937484.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            15577..17187
                     /locus_tag="ACG9CY_000956"
     CDS             15577..17187
                     /locus_tag="ACG9CY_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4937485.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            17344..18192
                     /locus_tag="ACG9CY_000957"
     CDS             17344..18192
                     /locus_tag="ACG9CY_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4937486.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            18423..18902
                     /locus_tag="ACG9CY_000958"
     CDS             18423..18902
                     /locus_tag="ACG9CY_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS4937487.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            18935..21163
                     /locus_tag="ACG9CY_000959"
     CDS             18935..21163
                     /locus_tag="ACG9CY_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4937488.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            21292..22935
                     /locus_tag="ACG9CY_000960"
     CDS             21292..22935
                     /locus_tag="ACG9CY_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4937489.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(23008..23553)
                     /locus_tag="ACG9CY_000961"
     CDS             complement(23008..23553)
                     /locus_tag="ACG9CY_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS4937490.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(23576..24061)
                     /locus_tag="ACG9CY_000962"
     CDS             complement(23576..24061)
                     /locus_tag="ACG9CY_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4937491.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(24051..24863)
                     /locus_tag="ACG9CY_000963"
     CDS             complement(24051..24863)
                     /locus_tag="ACG9CY_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS4937492.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(25002..>26112)
                     /locus_tag="ACG9CY_000964"
     CDS             complement(25002..>26112)
                     /locus_tag="ACG9CY_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS4937493.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         8531 a         4770 c         5218 g         7593 t
ORIGIN      
        1 atatcaagcc gactgctttg gcgaatgttc gcgcttttta tacacgtttg aaaccagaaa
       61 tccaaatcat tggtacaggt gggattcgga caggacaaga tgcatttgaa catctgctat
      121 gtggcgcaag catgttacag attggtaccg agcttcataa agaagggccg gaaatttttt
      181 ctagaatcat aaaagaatta acgcagatta tgtcagaaaa aggatatacg tcaattgacg
      241 aatttaaagg caaattacgc accatttcat agcaaagaga cgaaggtcat gattgatctt
      301 cgtctttttt tataaaaaac actcgtctgt tcattcctgt gcaagcgtac tcattgtatg
      361 ttagacgaac ttgtgggaaa ataacagagg caaaaaggag gataatatga atgatgcaac
      421 agaattagca aaagagctta aagaaaaaga atttagtttt caagaactag ttacggatat
      481 acagaaaaaa ataaagaagc aaaatcatga attgaatgcg tttgtcgcgc ttgaaccatt
      541 cgagagtaat tcccacgagc tattgattcc ccagtcagaa aaagggccat tggctggtat
      601 cccatttcct ttaaaaatgc tcggtcaaga aaaaaaggga tggctagcta cttcaggatc
      661 tcgtttattt gaaacacacc gagcttctca tacatctaat tatgtgcggc aagcagaagc
      721 gataggtcta gttccttttg gacaaaccaa tgcgccagag tttggtttca aaaatattac
      781 agacccagtc atctatggcc cagcaagaaa cccttggaac cttgaatgta caccgggcgg
      841 atcaagtgga ggagctgctg ctgcagtttc atcaggaatc gttcctttag ctggtgctag
      901 tgatggcggt ggttctatta gaatacccgc ttcgttttgc ggattgattg ggctgaaacc
      961 ttcgcgaggg acgatgccag ttggtccata tgcttggaga ggatggcaag gagctgctat
     1021 tgatttcggc ttgacagttt ctatgagaga cacggaggct ttattttatg ggatgagaag
     1081 tatccattcc ggtgcgccat atcaagtatc gccagttgag tggcaaacac atccaagaaa
     1141 aaaacggttg aagatagctt tttgtgttga ttcaccgatc cactcacaga ttagtgaaga
     1201 ggcagtactt gctgtaaaaa aagctgtaga tttccttgga aaagaagggc atgatttgac
     1261 agagattcct tacccactag atggtcgtaa actgatcacg tcttattatc agatgaatgg
     1321 cgcagaaaca gcagcaatga tccgctcgat tgaaacaggc atccagcgag ctgtcagaaa
     1381 agaagaactt gaacccattt cttggacttt gttccaatat ggtcagaagc tctctgccgc
     1441 tacttacatc gattctctgc atgtatggga tgaagcagca attgtcatgg aaaacctaca
     1501 tcaagaatac gatctattcc tttcaccaac gactgcattt accgccccta aaatcagcga
     1561 agatttgcaa agtgatgcga taagagcaca gatggaacac atcagtgatt gttctgaagt
     1621 agaaggttta gacgttgttt cttccatgtt tgaggagagc ttgaagatca caccttatac
     1681 acagttggct aatctgactg gacaaccagc aatcagcttg cctactcatt tgacaaaaga
     1741 aaacatgcct ctaggaatcc aatttatggc tgccagagga agagaagatt tgctatttca
     1801 aatcggctat ctatttgaag caaatcatca gttccattta ccaaatccat ggaaatagca
     1861 gaaacaagga gaagaagaat gaagaaaaaa agattgtttg ctctaataac tagtgccttt
     1921 tttattttaa gcgcttgcgg tacagctgcc gctaatcagg aagataacag ctggagtaat
     1981 gtagaaaaaa agaaagaatt agtagtagcg acttctggca cactgtttcc cgcatcttat
     2041 tataatgatc aaaatgaatt agttggttat gatgtagatt tggcaaaaga ggtggctaaa
     2101 cggttaaatg tcaaaatctc ctttaaagaa tacaacgtgg atggacaaat cacttctgtt
     2161 tcaaaaagag aatcagattt tgctgcgaac gattttggta tcacaaaaga aagagaaaaa
     2221 aaattctccc tttcagagac tttcaaatat tcatttgaca gtatgatcgt acgaaaaaaa
     2281 gatgattcag gaatccactc acttgaagat cttaaaggga aaaaagcagc tggcgaaccc
     2341 aatactagct atatgcgttt agcggaaaaa cacggagcta aattagtcac atatgacaat
     2401 gccacgaatg atcagtattt aagcgatgta gcaaatggtc gaacagatgt tattctaaat
     2461 gattattatc tgcagaaaat ggccgtatct gctttaccag atatccctgt aaaaatactg
     2521 gagaacgttt atttcaatga aaacgaaagt ggttttttat tgaaagaagg aaatgatgct
     2581 ctgaaaaaga aaatcgatgg cgtattaaaa gagatgaaag aagatgggac gacaaagaaa
     2641 atatctgaaa aatattttca aactgatgtt tcagtcaaac cgaaagaaac gatggctgag
     2701 tcggtgagtg agtgatgtac tttcctctaa ttgatttttc cctcatgtgg acttcacttc
     2761 ctttcgtact gcaaggcttg gtatacacat tagggattgg cttcgtgtcg tttcttttag
     2821 gaaatctggt agggttgctt ttgacagttt taggtctttt ggattggctg cctctgaatg
     2881 tatttattcg tttttattta tcgttcttta gaggcattcc cgcactagtc ctgctgtttc
     2941 tgttatattt tgggttacct tatcaattaa gtgccttaac ggcttcagtc atctgcttta
     3001 cgattaccag cagtgcattt attggtgaaa tctatcgagg atcactagca ggcgtttctt
     3061 caggtcagtg ggacgctggt ttagcttctg gtttttcttt ctttggggta ttgaaatata
     3121 tcgttttgcc gcaagctttt cgaatctcag taccggcact cagtaacgta gctatggatc
     3181 tagtgaaagg tacttcgtta gcagcaacaa tcacagtgcc agaaatattt caaaaagcaa
     3241 agatcatagg cggtcggaca ttcgattatt tttctctcta tattttagtt gcactgattt
     3301 attgggtcat ttgtatcggg atcgaaaaag tgcaacagta tttagaaaaa cgttctatgc
     3361 atcagtcaat ctgaaaaata gaaatggtgc tagcgtggct ggcaccattt ctattttttg
     3421 tttcttgtat attctgccta gtattttaaa accatttcat gtaaaatgta aatcgaaaat
     3481 atgttcggag gtagaaaaca tgaagacccc gctaaccatc gaaatagaat cctcgttata
     3541 ccaatattgc atcgagcaag gcggcttggt tgtagaagaa gtcacgatgc ctgatgacca
     3601 aggaattgta gatacgctta gctgcttgac aaaatttgat ggaagccgag aatggcgatg
     3661 ctatgagctg aaagtgacaa aagcagattt tcgctctaca gccaaactgt catttgttgg
     3721 acattataat tattttgttt taccaagtaa actatatgaa gaagtaaaaa ctgaaatccc
     3781 tacaggaata ggtgttttag tgtatcgtcc gtacacggtt gaagaggaga tgcctgcctc
     3841 tggtacattt ctgattgcga aaaaagcagc aaagaaagag cttgctgtac cggaagaagc
     3901 tttgacaaac cgcttcatgg cttctttatt cagggaagta agaaaagcaa agcaaatgga
     3961 gcgaggcgta aaatattttc caacagatca attgtacaaa gaattgaaaa gacgttcaga
     4021 aaatcaagat ccattttcta acgaacgata ttatgaacgg tttttagagg aaatagctag
     4081 tcaggcaatc aatgagttgc aagaagaagt tatcgcgctt cagcatgatt atgaatattt
     4141 gaggcaacaa cagcagatcc gtcggttgcc tacagagcca ttggagtgaa aaatatgtac
     4201 tatccttatt taagaggaaa acaatttgac ctattagcat taaaagaagc actgtcgcga
     4261 ggattgctct ccaataaaat ccaaccagtg atcgaaccag tacgtgattc cgctacgctt
     4321 aaaaatgtga tcgaattatt tcaaaaaaag caccatcgaa tcgctgtgat ccaaaatccc
     4381 caagtaggac aattcaaatt attcgatcag catgtgcaca gttggacagt caaagaaaat
     4441 agctctgtcg tccctgcaca gatcttaacg ccagaaaatc tcagtgaagt actggattca
     4501 cctccagcgt ttcttgtttt cgatggacag cactacccaa aagatacaga ggtgtggaaa
     4561 cagttggcag gagttgattc gaaatttttg ataccagata ctagtcgctt tcgcatctgg
     4621 ctgcccgaaa acaaaattgt gatccgtgac tcttttcaaa cgagaaagca tgttgaaagt
     4681 tatgcagata aaacagatga cttttttagt gatgactatc tttttttcca cgcagacgga
     4741 tatattggat tttctgattt cacgatcgaa ggtagccgtt atttcgataa agggttccct
     4801 agccgagcgc ttgctatcca tctaacgtat attgatgctt acggcaatat acgcgtgaaa
     4861 cattttgtct ctgacagtaa cgactctgct aaagaccaag cattgaagtt tcttgaagca
     4921 ggagataaaa tgaaaaaatg gatcatgcgt caccatcatc aactcttgat tacttcagga
     4981 atgattgaat tattggctct ttaccagcaa caaaaattcc ctggactggg agtgttgaaa
     5041 aagtggtcac ttatgcatca tttggaactt attagtcacc tgctagatca tccaaaagat
     5101 tggcttaaat attattcaaa agttcaggaa ctatatgaag tgatccaatg attagataat
     5161 gtaaatgaaa aaggaataga aaagaggaaa ctggatgtat attcggcaaa caaggaaaga
     5221 agaagtagaa gaagtggtag aaatcattaa tgccgcagcg gatttcattg cttcgcaagg
     5281 ctctcctcaa tggcaaaacg ggcagaaacc aacaaaagaa acgattttga acgatattga
     5341 aagaaaagaa agctatgtat taatagtgga agatcaaatt gctggaacag ctgcattagt
     5401 ctcaagcgtg gatcccgttt acacaaaaat agatggtagt tggaaagaat caactggtcc
     5461 ttatttatcg attcatcgtg tcgctttagc agaaagtact cgaggaaaaa aactagcaaa
     5521 accgttctta gaacatttgt tgacgattgc agccagcaaa aatatccaag atatccgtat
     5581 cgatacttat ccagctaatg aaccgatgga aaagaccatc tattctgctg gattcactta
     5641 tcaagggatg attgaatttc cttttgaaca cggcgaaaga aaagcatatc aaataatcat
     5701 caattgacta taaaatcatc tgagcaaaga taaagacatt aacactactg aaaaaaacaa
     5761 tcatatgata gccaagaggt tgtaacagaa gtggccagct tcaaaaaaca agaaagaatt
     5821 tccgaaaatg cttctcaaat ttttgtggaa ttttagcgta ttttacgaaa tagctgcttt
     5881 ctgaaactcc cttcatcgag aggtcgtgac atagcttttg tcacgacctc tcggcttatc
     5941 ctacgagtat tattcgatac atttatatca ataatccttt ttttatttta tctgcagctt
     6001 cttggaacgc tccatcgttt gacacgacta aaagtgaggg gatagctttc aaacgtttca
     6061 actgtgctaa atcttctaca taaaccgcca gtctggctga aatctgttca gatgaatctc
     6121 cacgtttttt caggcgcgat tgcaatgttt ctttagagat cgtaataaag ataggacgga
     6181 ctaccgtccc gaaatactca tataaatgcc aaaaaccatt tgctgtcaaa acggcgtaac
     6241 aatttcctac tgtcagtttc tttttaattt ctgctttact ggaaccatat ttatgttgag
     6301 catattggtc ccactctgca aactcatttc ccttgatcat ctgattgaat gtattgtcag
     6361 aaacaaaata ataatcctct tgatcttttt ccccaggtct tggtgatcga ttcgtgtaag
     6421 taatgatctt ttgttccggc tgaaaaatag cttctgcgag tgaagttttt ccagaaccag
     6481 aaggaccgat aaatacatag caataattca ttgtcatttc tcctttgatg attgaacaag
     6541 tgaaaaagct ttggaaaata tttccaaagc ttttaaaagt aactttgctt ttttgtatat
     6601 tataccagca agccagtcac aatagcactt aaaaagctga ctaaagtcgc tgttagcaaa
     6661 attttcaatc cgtgttttgc gacaagattt cctttttctt catttaatcc cttcatcgca
     6721 ccagaaatga tcccgataga agagaagttg gcaaatgaaa cgaggaagac agatataatt
     6781 gctgttgtac gctcagtaaa atggaaatta ccatttgcta attctgtcat agcaacaaat
     6841 tcatttgtaa ccagtttagt cgccatgata ctaccggcat ccactgcttc tttccatggg
     6901 attccgctga tgaaggcaag aggggcaaag atgtaaccta ggatctcttg gaaagtgatg
     6961 ccgaatatcc cgtggaagat agcattgatc atggcaatca aagcaacaaa accgataagc
     7021 atagcagcaa ccgtgatcgc cacatggaaa ccatcaagaa tgtattctcc caacatttgg
     7081 aaaaatgtct gttctttgtt ttcttcaatg atcagctcat cttctttttc ttctaataca
     7141 tagggattta caattgaagc cagaatataa ccgccaaata agttcaaaac aagggcagtg
     7201 atcacatatt tagaatcgat cagtgccata taagatccga cgatcgacat agaaactgta
     7261 gacatagccg atacactcat ggtgaacaaa cgtttctcag ataggaaggg taattccttt
     7321 tttatggaaa taaaaacttc cgattgtcct aaaatagcag aagctactcc attataagat
     7381 tccaatttgc ccatcccgtt gattttactt aatcctagac caacagcttt cataaataat
     7441 ggaagaatct tgatatagcg tagaataccg ataatagcag aaataaatac aattggcatc
     7501 aatacggaaa gaaagaaagg tgcacttcct ttatttgcta caccggcaaa aacaaagtca
     7561 acaccttgcc cagcaaaagc aagaagatga tcaaacattt ttgccaatac agaaacaacc
     7621 gctgtaccaa aagttgtacg taataatacg aaaccaagaa caagctgtaa aacgaacatc
     7681 acaattagtg gtttcaattt gattgctttt ttgttactgc tgatcaaaaa agcaagccct
     7741 aatgtgacaa ataaaccaat aatacctaat aaataactca aataatcact cctattccaa
     7801 attccgtttt attttacctg ttttgcaaat aaaatactaa tctttagtga aattttcatt
     7861 acttttttag agtgaaacgc attcattttc cattagaaaa taaagcgaac aaaataaaag
     7921 gtttaaaaag tgtaacgtct tagtaaagtc tataaaaaaa cgaaactatc gattaagcta
     7981 atagtttcgt tttcaacatt tataagtaaa ctttttctat tggtaatata gattttcgga
     8041 agggtagaca ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt gttcatcatt
     8101 tttattgacc atcacagtag agtgggcttg tgctccctca agttcagcta gtttatcata
     8161 agcaacttga gcgattggat tcgttactgc actgattgat aaagcgatca agacttcatt
     8221 gatgcttagt gcatccaaag aggaatgaag tgtctttgat ttcaatcctt gtattgtttc
     8281 aagaactaat ggtgacaata aaggaatgtc atctgcgata tgggctaaat gttttactgc
     8341 atttaagata actgccgctg acgcatccat caatgaactt gtacgcccgg taatgatgac
     8401 ttcatctggt aattcgattg caatcacagc agaaggatct tttgtaggac tttgcgcttg
     8461 aatcttatct gcatagtcat gggcagggac taccacgttt cggtcttcag gacgtaaacc
     8521 gacttcttcc ataatcagtt tgattcgatt aactgcttct tcgtcgatca atcctttttt
     8581 gaagtcgcat tctgtttgga aataacgacg gatgatttct tgtttagagg cttcttggac
     8641 aacggtatca tccacgatcc caaatcctac acgattcaca cccatgtcag taggagattg
     8701 ataaacagat tctttaccag taattttttc aataatacgt ttgattacag gaaatgtctc
     8761 aacatcccga ttatagttca cagctacttt attgtaggca tcaaagtgga aggaatcgat
     8821 catattgaca tcttttagat cgactgtggc tgcttcataa gcaatgttca gcggatgttt
     8881 caacggaaca ttccatactg ggaaagtttc gaacttggag taaccagctg catgtccttt
     8941 ttggctttca tgatacagtt ggttcaagga agtagcgagc tttccacttc ctgggccagg
     9001 agctgtcact acaacgattg gtttagttgt ttcaatatac tcattttttc caaaaccatc
     9061 ttctgagacg attttttcta cgttgactgg atagtcttcg atttctgagt gtttatagac
     9121 cttgatgttt cgtctttcta gtttattgat aaagactttc gtggcaggct gtccactata
     9181 gcgagtgata acgacactgt tgattaataa gccataacca cgtaattcat cgattaaccg
     9241 caagatatcc atatcataag taatcccata atcaccacgg atcttattac gttcaatatc
     9301 tccagcatag acgcaaatca gtatttcagc ttgatctttc aatttttgca gcaatttgat
     9361 tttcgcatct tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt caactagctt
     9421 cccaccaaat tctaaatata atttgtcata gtggttcact cgttccaaaa tatacgctga
     9481 ctgctcttca atatattttt ctgaatcaaa accaattttt ttcactaggt tccctcctta
     9541 agtactaaag cattttttca tttttttgag gattcgtcaa ctggaagagc gaaattcctc
     9601 aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat agttattcat tggtatgata
     9661 gtttttgata aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac gcatataaac
     9721 actatatccg ttcgattttt accggttatg aaagaatgct tgaagatccc gaattggaac
     9781 aattgacgtt tacttatcta aacgaagaaa cgcagctaac tcgtgatgag catcagcgca
     9841 ttcatttcac aacaagagat ctgcctagta aataaaaaat aagataaagg tcatcggatt
     9901 cgattctgtc ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt accaaatttt
     9961 ggtgcagcct gttttatgga gcattgcccg ctctttttta taacaatttt ttacagaaaa
    10021 tggtactatt gaagtattaa taagtgaaaa acagatgagt aagaggaggc aagtagtatg
    10081 aaagagccag atacaaatgc aaattatgag ccagacgaac tagaggagac gaatgcggat
    10141 caggatacag gacaagaatc gtcttctttt tacgatatca aagaaaaagc aagcatgaat
    10201 ctttcccaat tattagaaga cttacaggct tttgaaaaag ctgtccgcga agaagatatc
    10261 cctgagatct accgtttata caatggccga ttgaatgaag aattaaaaag aagctcaaat
    10321 gaaaatcacg agatcgatca attgctgatg cgtaagatcc atgatcgttt tttacaggaa
    10381 tttcctttta tggaacaagt agaaaacatc tcgccaacga tgagctatta caaaatcggc
    10441 acttattacc atgatcggcc cacggtcggt attgatgcaa gtctgccaga aatttttgtt
    10501 ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac cggagtcaac ggactatgac
    10561 aaaaaaatca atgagctaga cgcaaaggtg atcactgctc agacagaaat tgaacgttta
    10621 gatgaacaaa taaaagaaat caatcgacaa atgactgatt tgaacgacaa taaaggattc
    10681 ttgaatcgta aaaaaatcga ggaagaaatt caagaattag agaagaagaa gcaagtacta
    10741 tctaatgaaa aattaggctg gcttccttat attgagacgc ctgaaacaat ccagcagcaa
    10801 aaagaagcat tgaagcaaga agcaagagca gaccagcttc gtgcagccat cgtagaaaaa
    10861 gaacaaaggc aaatcagacg ctatttcggt agcaaagagg gattcggtca agcaatccat
    10921 gaattcttga tgaattatct tggcaatgag aatcctaata atcaatctaa tgaaggaggt
    10981 tccgagtatg aatgaacacg atgaagcaga atggacaaaa aatgaagaac aaacagaaga
    11041 tcatgaagaa acgaacactt cattggaaga acaaacaagt aatcctctgg atgacacaga
    11101 agaaatccct atgctagagg aaacaaaaat acacgaaaaa gaaaaagtaa ctccgacgaa
    11161 tacgaatact gcagaaaaca cccatgctta cgaaataact gaaaatcccg ggtatttaga
    11221 aggaaaagag caagatcatc aagatatcgt cagtttgatt gaaacagcgg aagcagaatt
    11281 agcggattta cgtatgagaa aagatttgat cgaatcagat tttccacaat tattagaatc
    11341 atatgatcgt tttatattga atgaagaacc aatctccacg atcggaaaga caagcctgat
    11401 cgaatacttc acgtatgaat tactgaaacc gttgaatgat attggtctgg aacggtcaaa
    11461 ttcagcttat gacacatgga aaacaagaca tttttcgatc aattatacgt tgaatggaca
    11521 aaacgaatta gtcttttcct ttgtattacc gaccatcgat caacgatacc aagtggaatc
    11581 gatgagtctc ctaaacgtat caccagagac aatggaaatc aatgtccaag atgaccgggt
    11641 gctttctttg atccgacact ggtcagtgga tcgagtattc tctgcagggc agatcacgat
    11701 attcaatcac aaattgaatc aattattagc ccatgcaaga acacttggtt ttaccgttaa
    11761 tcagacatta ctagacaata cgaagccact tcgtttgaat cttcagagcg aatttgaatt
    11821 gacgaatgaa gtattggatg atatttttat cgtggcaatg aaaaatccat cctacgatat
    11881 ggaaaagatc aacgaaacta cttatcaagt acgattagat aaaggacagt ctttaacgat
    11941 aacaacaaac gaaaaagatc aaacagtctt gtcgatctca tctggagact acccacgatc
    12001 tgtcattgat ttctttatca attacgaatt cttggtccca ttaatggtga gaaagtcctg
    12061 attggataag ttggattacc agatgtcatt cagataattc tgttccaaag gacttgcggc
    12121 agttatccag aataattgtc acaagtcttt tctgtatgta ctaaatggac gtgaataact
    12181 tgctgatgaa ggagggattt cattaggaat gtccacaaat aaaatagcac agcttagctt
    12241 attgtctgct gcttgtgtag caggtaggat cgcttttcaa tttattccga atttccagcc
    12301 aatgactgct atttttttat ttatagtact ttacttaagc ttaaaagatg cgttaattgt
    12361 catgagtctt tctattgcga tcagcagttt ttattttggt gttggaccat gggtcatcgg
    12421 ccaatggatc agctatactg ttgttctgag tttgttttca atcgtctgct tgagaaaaac
    12481 agtgcaaagg aatctatggt taaagggagt ctgttttttt ctggcgggca tggtttacgg
    12541 aataggtatg accgtttttg atacgctgtt atatcgtttg cctcaacctt ggatctatta
    12601 ccttcaaggg gtgtcgtttg atttgatgca cagtataggc aatgttgtgt tctttcttgt
    12661 ttttctgcca gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg aaaaaaataa
    12721 tttatagttt gcttgcaatg agtgtcagta tactcatttt gacaagttgc agtacaacct
    12781 atacacaatc ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa gcaacaatca
    12841 cgttacaaga aaatggtcaa aacttttcgg aaaaagaagt gttcttcaaa aaaggcgatg
    12901 atttactgag tattttgaaa cgaaattttg aagtcaaaga agataacggc ttcatcactt
    12961 cgcttgaagg gcactcacag gatgagaaaa atcaactcta ttggatgttt actgtggacg
    13021 ggaaaagtgc tactactggt gccaaagaga tcaaattgaa tgatggacaa tcggtcattt
    13081 tcaatttgtc taaactataa gtttcataaa aaactgttaa agggcgaata ttgccataat
    13141 tcagtgagtt cagttgatga aagaaggaaa ataacatgga aaattttaaa aaaagcggaa
    13201 tgttttccgt aattgctgct ctaggggcta atattctagt tgctatcagc aaatttgtcg
    13261 gctatgcaat cagtggcagt gccgccatgc taaacgaaag catccacagt atcgttgatt
    13321 gcagcaatca gattttgctg ttattcggtg ataaacaagc caataaagga cagagcgagt
    13381 tgcatcaatt tggtgaagga cgagcaaaat actttttcag tacaatcgtc gcaatgatgc
    13441 ttttcttcgg cggtggggca cttggggtga tggaagcttt tgaaaagttg acgcatccat
    13501 cccatgaagt gggtaatccg atcattgtta tagcaatact gttatttggt ttggtaatcg
    13561 aaggaagttc gttacgcgtg gcaatgaaag aaatcaaaga attgaatact gaaaaacttt
    13621 ccacgatgcg ctttttgcgc gaaagtcgac acagtgaaat actgattatt tttacggaag
    13681 acttgtgtgc tgtaatcggt ttggtcttgg ctttagcagg aacaatattg acttatgcaa
    13741 ccgggattgc tgcatttgat gcgatcagcg gattgctgat cggtttcttg ctgatgggag
    13801 ccgccatttt cctagcaaaa gaattttata gtctgatcat cggtgaaagc gtgacagcaa
    13861 gcgatttatc aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg ctgatcgata
    13921 taaaaacagt tcatttaagt ccaacggaga ttttagttgc agcaaaagca gatatcgttg
    13981 gtaacaaaga gaatgaagct tactcggtca tcaatgatat tgaaaaaaat atgcgtcagt
    14041 ctcttcctga caagaagatg tatatataca ttgaaacaga taaatatgac ccaaattatt
    14101 ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat gagaaaaata taatcttgtt
    14161 tttaaataaa acggctacaa tttagcgaat ttatgttaca atgagtccgc tgtttatttg
    14221 ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg cttcaagctt
    14281 tgcatgcagt gatgccactt tgtataagct atataccagt aggattagct tgcggagtat
    14341 tgttgcaaaa agttggattt gatcctctct tatcaggact gttatcgatt ctggtctttt
    14401 ccggtggcgc gcagttttta gttgcatcta tgcttacgac gcaagcatca tttgcaacga
    14461 cattattgat ggttttcttt ttagaattac gctatacatt attaggagca agtcttgccg
    14521 gttttatgaa aaaagaaaag cggacgtttt tggctgtctt ttctcaatca ctgaatgatg
    14581 aaaactatgc ggtgaactac ttaaagtttt caactgaccc ttcctggaat aaaagaaagg
    14641 ctttatatgt caattggttc tcaatgactg catgggccgg tagcaatatg atcggtaatt
    14701 ttttcggctc ggtgattcga gtagatgctg accttgtcca ttttgctttg acggccatgt
    14761 tcattttcat gtttatcatg cagatgaaaa gcgcactact gatctttaca ggtttattct
    14821 caggcgtatt aggggtcgta ttcatggtat tattccaaaa tactttcgga ttgattgcag
    14881 caacagtcat tgcttcattt gccggattta tgctggagaa ggcattcaaa aaagaaaaag
    14941 ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac ttcccggaac
    15001 aggaggccaa tcaccatgac taaactatat ttgctacttt tagttgcttg tctgttcatc
    15061 gtggcatata tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa agtgccatcc
    15121 tggttcagtg actggatgaa atatgtaccg gtggcattat ttgcggcgtt agcttttaaa
    15181 gatgttttca ttactcatga acatctggat attgcttgga atatcaaaat agctgcaatg
    15241 attctagtag caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt tttgacagga
    15301 cttgcgtctg tctttttgct gtctatgctt taaactgttt tattttatta aaaacagaga
    15361 ttgcgacata agttgttcaa actgatctat ccgaactgca tttgcaacag tcaattctgg
    15421 atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc aagctctttt
    15481 ttattttagg aattagagag ggatttgtcc ttctatgatt ttttgctata atagggaaga
    15541 tgtttatttt atgaaagaat agttgaggga atttaaatga ataaaaaatt gatgcaagga
    15601 acattttggc taacctttgc taatcttcta tgtaaagttt taggggtagt ctatttgatt
    15661 ccctggctaa gtatgatggg gaacaatcaa gatggtatgc ttgctacgac attgtataat
    15721 gttggctatc ttccttatgg attgttcctg atgcttggta cagtcggatt tccaaatgcg
    15781 attgcaaaaa aagtagctgt agccacaaaa gaaggagata acgctgcttg tcggctgatt
    15841 tttagaagta cgatcaatat catgtttgtc atcggtatcg cctctgctgc actgatgtat
    15901 ttatttgctc ctttactagc cggaatcagt ccgattgcta acgttaataa tgggatacta
    15961 gctatcagaa gtttatgccc atctttgatt gctatcccaa ttcttagtgc aatgagagga
    16021 tatttccaag gaaaaaatga tctgcggcct tatggaactt ctttgattat cgaacaggct
    16081 gtgcgagtga tcgttatctt ggctggtact tattacttgc gcgtattgac tgacggaacc
    16141 atattagaag ctgtattgat cagtacagtc gcttctttct tcggtgggtt agctgcgatt
    16201 atccatatgt atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga ttttttgatt
    16261 tccagccgtt atttcgaaag agaaaaccga tctgcctctg tctctatcat tcgggaaaca
    16321 ctaccgttta tttttgtcgg ctcagtcatt actatcgttc aaatgatcga ccaagtaacg
    16381 atgaaaccac tgcttcactt tttccgtcca gagatcgcag atcagcaact ggaattctta
    16441 ttcagccgtg cttctgtcaa tccaaataaa ttgacattga tcctgatctc catggtagga
    16501 acagtagcta taagcagctt gccaatatta agcacgatga agaaaaaaga tcggatacaa
    16561 atcgaaaaaa cagtaggaga tagtttttcg attgcgttat tgattcttct tccttcattg
    16621 actggaatgt ctttattagc tggaccacta tacacattgt tttttggcta tgatcctgaa
    16681 agtgtcggat atttccaaat ggctttgcta gcttctttgt ttttctcttt attcacgatc
    16741 ctatctacga tgcttcaatc tttgaatcac catcgttttg cgatccgatt aactgtagaa
    16801 gcaattatcc tgaaagtcat cttccaagta atcggattag gattagttgg cggatacggg
    16861 atgagcttat caaatggctt agcattcggt attgtctttg ttcgcggcta tcactttatg
    16921 tgcaaagaat accgaatagc acctttagca aagatcagca attttttctt aaaaactttc
    16981 cgctccaccc tgattatgct tgcagtatgt tttgccgttt tctttttgct gaatcagcga
    17041 ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg tggctgtagg aagtattggc
    17101 ggcttagtat ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt gaaaaatgtt
    17161 aaacatcata gcagaagcaa agaataggtt aactaatcgt tttttgctgt cacttgaaaa
    17221 acggcaactc gtatgattac gcttgtcgtt tttctatttt ccaattttag tttcatatct
    17281 aatcttactt atgatatagt tataaggtct ggaaaaattc tttgaaacga ggaaattaaa
    17341 cacatgaaac ttgcagtagt tacagatagt tcggcatatc tgccggaacg tatcaagaac
    17401 catcccgact tattcgttat tccaatccct ttgattatgg atgggaaaat ttataacgaa
    17461 ggaatcgata tcgaagcgga tgaatattat ggacttttaa aaaacagtaa agattttcct
    17521 actacttcac aacctgtcgt aggggaagtt ttgggtttat atgaggaatt gaaagagaaa
    17581 gggtatgaca cgattttaag tatccatctt tcttcaggga tttctggctt tgtcaacaca
    17641 ttatttgcga tgaaagatga catcagcgga gtaacggtca ttccttatga ctctaagatc
    17701 actagtatgc cgatgggaca catggtagaa gctgccttag acttaaatga tgaaggtcag
    17761 tcccttgatg aaatacttgc acatatcgat cgaatccgag ataataccta cgcttatctg
    17821 attgtagatg atttgaacaa tttagtacga ggaggacgtt tgacaaatgg tgcagcgctg
    17881 attggcagct tattaaaaat caaaccgatc ctgacatttt ctgaagggaa aattgtttta
    17941 ttcgaaaaaa tccgttcaag taaaaaagca tttgctcgag ctgaagaaat tattggtaga
    18001 agaaataacg aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa taatttagaa
    18061 gtagcgattg cggagaaaga gaaattgcag aaaaaatatc caaatgcaga aatcgaaatt
    18121 ggtcatttcg gaccagtcat tggtacacat ttaggtgaaa aagccatcgg cattgccatt
    18181 tcagcgcaat aattcctttt attcaaaaaa cataccaaaa atattccaac atcatcttgg
    18241 actttctaca tgttactaat gagcgtgtaa aagaacgaga tggtgttttt atttctatca
    18301 atgaagctct ttttctttgc aatcctcact tcaatcctct ataatgtcgc atatagttta
    18361 gtaaatgagt aaactactaa tttacttatt tactaaaaat aaaaggatga ggagttaaaa
    18421 taatggaaat accaacaaat ttaaaacata aaccagtaat cgttgtagaa gactatgata
    18481 aagttgatgg aagaaatgca ttaaatacgg atgcaaaagg gctttcactg ggccttgctc
    18541 agtggaatga tcgtggaaaa gtcgatatct caggcaaggt atggcgacat actggagaaa
    18601 aatggtcacg acagtcggaa gaattaccaa tcacacgtat tttagattta gcgatcttag
    18661 ctgcacaagg aagtttatac ttccaagatg cttatcgtta tgaaaaattt tatgatccag
    18721 aaaatccagt cgtagacatc attggattac aagggaaccg aatgacggta gaagtagata
    18781 ccaaaaatcc aaagatcgat gaagacatca ttttgtatta cgatacacta caaaaagacg
    18841 gagaactgat tggggaacga ttccgtattt taaaacgtct tttagatgag ctaggatatt
    18901 aatagacaga gatgatcaaa aggggaagag aagcatggag aatttctcac taacagaaaa
    18961 ccgaaccttt tcacggctag aaaagcaaat gatctggaag aaaccgaaaa gccatcggac
    19021 aagtgcagaa gaatggcgga tcgcctccga aatcaaaggg aattggcaag atcctgaaat
    19081 gaaaatcagt aacgtacttt tggaaggaga tgccgggtcg gggaagacac aattagccaa
    19141 agcactatct gctgatctgc aacttcccta tacaaaagtg acctgttttg cagatatgga
    19201 caaatcagat gttttcggag ccttattacc tgtagtagat tctaataagg aacaagacca
    19261 agaattacta gaagctattt accaaacaga tacacttgaa gctgtactga cactgatcca
    19321 gaaccattac ggcattgatc ctttttctgc taaagaaaaa ttagcagagc tagttcaaag
    19381 gatcgaagct gattatccag atgcaatcca ttacagctat tacccttctg aaatcgttcg
    19441 agcgattgaa aaaggatatt tattagagat tcaagagccg actgtcatcc gagatgcctc
    19501 tgttcttgtg gcacttaact cagcgctcga accaaatggg atgttgaatt taccgacagg
    19561 aatcattcgt cgccatccag attgcgtcat tgtgatcact acaaatcgaa attaccaagg
    19621 aaatcgccca ttaaatgaat ctttaagaga tcggatgcaa catgcggaaa aaatggatct
    19681 tcctgcctta gaagtcatgg cagaacgtgc gatggcgaaa actatgatcc aaaaacaaga
    19741 acttctctta aagatggcag agattatccg tttgttggat gaaactgcaa aagctaacgc
    19801 catcaaaggt gtagcaggaa tgcgttctta tttttattgg gcaaatacct ataagcaagg
    19861 acaagattta ctccagtcga tttatccaaa agttttgtat aaattatcca ctgattcaga
    19921 tgaattggca attcttgaac aagctttgtc tgacagtgga ttgctactgg aacttcagga
    19981 catattgaga aaagaaaaat ggggtcatcc tcaatctgag catacaaaag gaagaaccat
    20041 tagcgccgaa gaagcaaatg aacgaaacat cactgaaaat gctgaagaaa ttctgcgctt
    20101 agatgaaaag aaatcgttgt ctgctgaaga accaaatgag ggagaagaga aacaagaaga
    20161 aacaatagaa aaaaacgaga ttcctcctca aatgcaaaaa gaacgcagta gcgatatcga
    20221 aggaaaaaat caaaaagaac aacaaacagg agatgattct caagaaatgt ctgcttctga
    20281 catggaagca catgataagg aaatcaagaa gcaactgaat aaagaagcgc gacaaatcat
    20341 gaaggaaacg attcatgaaa aagaaggtct catcgttcac cgaccagctt tccaaggaaa
    20401 aacgccggaa gcacgtgagc tacacatgca agtaatgcca atagtagaat cacttagccg
    20461 gcaaatttta gagttactgg ataatgaaca aacagagacg taccaaaaag gaaaatacga
    20521 aggtcagcgg ttcaatgcaa gcaaagtagc atatggtgat ttgagaaatt ttgacaaaaa
    20581 gaacccgcca catgagcaac cttctttagc ggtagctgta cggatcgatg aatctgggtc
    20641 aatgatacgg gatgatcgga tcgaagcggc aaaaaaagca gcgattgcca ttgccgaatt
    20701 ttctaagaaa gtcgatatcc cattgttgat ttacggagat acagcagacc gttcgtcaag
    20761 agaaaagact tctttgtttt cttataaaga atttgaagac ggatttcaat ggttaaatga
    20821 aaaattagtc actatgaaac caaggcaaaa caatcgtgat ggtgctgcac ttcgtttagc
    20881 agcagaaaaa ttaaacagac aatccgcaac aaccaaactg ttgcttaata ttagtgacgg
    20941 tcagccaaaa gcgttgccgg attatacagg caaaaaagca aaagaagata tccagtctgt
    21001 tttgaaagaa tatgaacgtc aaggaatcct atttgtctcc gcagctattg gccaagacaa
    21061 agaagaaatc aagtcgatct acggtgaaag tcgttttgta gatattactg acttgaatga
    21121 atttcccaag caaatgattc aactgattgc ccgctatcta tgataaaata aagctcaacg
    21181 aacgcactta gcagattcgt tgagcttttc gtttctagtt accggtaaca aggatacttc
    21241 taaaatagaa tcggttataa tcaagaggga ccgttaaagg agtgaaacga tatgatacaa
    21301 gaacaaaaat ggtggaaaga agcagtaggc tatcaaatct atcctgctag tttcaaagat
    21361 agcaatggcg atggtcaagg agacatcaat gggatccgtg aaaaacttaa ttatttgaaa
    21421 gatcttggta tcggctttat ctggatcaat ccgatctatc aatctccatt tgtcgataat
    21481 ggttatgacg taagtgacta tcaagatata gaagagagat tcggaacgat ggaagatttt
    21541 gatttattgc taaaagaagc acatgaatta gggatcaaag tgattatgga tcttgtcatc
    21601 aaccatagtt cggatcagca tcaatggttt gaagaatcga aaaaatctaa agaaagcccg
    21661 tatcgcgatt attacatttg ggtcgacggg gtagatggca aagaaccgaa taattggaca
    21721 tcgattttcg gcggttctgc ttgggagtat tctcatgaaa ctggacagta ttatctgcat
    21781 gtttttgcaa aagaacaacc agacttaaat tgggaaagcg agaaattgaa agaagaacta
    21841 ttcaatatga tccgatggtg gttggataaa gggatagatg gatttcgttt agatgcgatt
    21901 tctcatgtaa aaaaagatga atattcagta aaagccacag agaacccttt ctcacccttt
    21961 caaaatgtct caggaatcga agaacatttg accgatttga aacatgtttt tgaagagtac
    22021 gatatcatga ctgtcggaga agcaagtggt gtcactgcag aagaaggacc acaatgggta
    22081 gggaaagacg gttatttcga tatgatattc gaatttgatc atatccatat ctggcagcaa
    22141 gaaaaagaag ggcaattaga tgtccttaaa ttgaaacttg cattaagcgc ttggcaaact
    22201 tcattggatg gtatcggatg gaacgcttta tatatggaaa accatgatgt tcctcgtgcc
    22261 gtttcagtgt ttggagatac tcgtcccgat ttttgggcta tgtctgcaaa agcaatcgct
    22321 atgatgtatt tctttttgca aggtacgcca tttatttatc aaggacagga aatcggaatg
    22381 acgaatatgc cctttgaatc gatcgatcaa gttgatgcag tagattcgaa aagactttat
    22441 aaaaggctgc ttgcggaagg taagacaaga gaagaagcat tggatatcat tcgtgaaaca
    22501 acgagagaca acagcaggac acccatgcaa tggacttcgg aacaatatgc tggtttttct
    22561 acacatgaac cttggctgat cactaatcca aataccaaaa cgatcaatgt agaacaacaa
    22621 gaatatgaac cagagtcggt cctgcaattc tataagaaca tgatccgcat tagacaaacc
    22681 aataaaggtc tgatatacgg ttcttataaa gaatatcttc atgaacatcc acaactttat
    22741 gtctatgaaa gatatttaga agatgaacaa tatcttatta tggtaaactt aacagaatca
    22801 cttgctgact atgaacttcc aaaagaagca gatcagtcct ggacattact cttgtcaaat
    22861 tcctctagcg gagaatttga agctaaaggc atactagcac cttatgaagc tcggttatat
    22921 aaaacaaaca agtaaaactt cctcgttaaa gttcattagt tggactttgc gaggaagttt
    22981 ttcagattgc tctgtcaatt actcatttca ataattcgct ctaggaccac cgatatattt
    23041 aggacgagag cgaagggctg tcacgtgagc ttgtccaagg gttttcaaac gaggtctgac
    23101 aggtacttgg acatgtttga tatgcatacc gattgatgtg tcaccgatat cgataccggc
    23161 ttgtccaact acgtgttcca cttcgacagg atcattgaac tggtcaaaag cagcaacaga
    23221 acaagcacct ccagcatgta gtgcaggaac aacagagaca atttcgaaat tttttgcttc
    23281 tgccactgtt cgttctacta ctaatgctcg gttcaaatgt tcacaacctt ggacagctag
    23341 cgcaatttct ttaggatcca acaactcttt taacgtacgg atgatccatt gaccaacttc
    23401 cgcactggaa ttttttccaa tatgtcctcc aacgacttcg cttgtgctgc atcctaaaac
    23461 aaaaagatca ccttttttca agtgtgcctc agccaatatg tcattaacta tttcagtcaa
    23521 ttggtctttt aattcttttt ctgacaactt cattttctaa aaccttcttt ttcaattaat
    23581 attgcttccg tgcttgaata cgttgaagac ttgcagttat aggaaaggca agtcccattc
    23641 caatcagatt ttggaagatg tttcctggta tagaagccac tccagccggc caatcatata
    23701 aaatccaacc ggcgatacca tagccgatga tcataactaa gcttcctaat aaaagcccta
    23761 atccccacat acgagtagaa gatttgctga aagaaccagc aacatatcct tgtaacccgt
    23821 ggatcactaa tgaaaaaact gcccactgcg gatatcctga aatcaaatcg atcaatagac
    23881 cactggaagc accaacaact aatccacccg ttggaccata aagaaaagca gcggtataga
    23941 tcccggcctc gcataatgtc acaaatccat tcgtagccgg tacagggatc aaaattgtta
    24001 atgaaagggc aactgtcaac gcaatcagca tagcatagat cgttaattgc ttagttttca
    24061 tttctcattc ctcctttttg aataccgcca gaccagacac ttccggtatg atcgttcatc
    24121 agcacgccat tagaaataca atgggcaaca aattttttac tttctgctac tgctttggta
    24181 aaatctgccc ctaacacaag attcgctgtt attgcagaag aaaaggcaca accggccccg
    24241 tttactgttg tagtgtcgat ttttttatga gaaaatacct cataatgatc aattgaatag
    24301 agaagatcca cagcttcatc tccagccatc cttgtgccgc ctttgatcac aatgtttttt
    24361 gttcctgaag cagtgagttc tttagcgaga tggatcattt ctgttaacga agagggaaga
    24421 gcttgaccag aaagtaaagc agcttctgcc aaattaggcg taatcatcgt aacaagtgga
    24481 aaaagttctt cgatgagctg cgaaatatac aattgattga tcacagcttc agtttctttg
    24541 aatgccaaaa cagggtctaa aatgattggg atattttgtt ttgtttttat gaagtctcga
    24601 acaaccatga ttgcttcact tgaattcaat aacccaattt tgataccatc taatgaagtc
    24661 attttatcaa tcgaaactaa ctgtcgttta agcaattcca aagatataga ttccagcata
    24721 aatcgctcat taatatctag tgcccctata gaagtcaagg cgcttagtcc gaatagacca
    24781 aaattctcaa aagtttttaa atctgtttgg attcctccac cagcaaatgg atcagaaccg
    24841 ccaattgtca agattctttt cataacattt tcctccatga ataagagtat aacgaaattt
    24901 tcaaaaaaga aaaacagcca attggcatat ttttatctca tccaatttaa aaaaacaaaa
    24961 agaaaagaaa gaccaaaatg gatctttctt tccttaaata gttaggactt ttgtgatttt
    25021 aattctttct cgatttgaag ccgttgcact ttcattgtag ctgttcgcgg gattgcatca
    25081 aaatcacgga tgatagggac gttcaagcga ggaagttcat ggacttgctc ccaccatcga
    25141 ttccaatcca tttctttatc aggagctaaa gcaataatcg gttgtggaga atgattttta
    25201 tcccttacaa tcactacttc tgccaaaaaa tctaacgaat ctaataaaaa gtcttcaata
    25261 gctaatgtac tattgatcgt ttcgatcaaa tccacttgcc gatctttcaa aaataggtgg
    25321 ccatgcttat ccataaaacc gtaatctccg ctatcccacc aatcgccata aacattttcc
    25381 tggaaccgtg catcttcctt gtaataagtc aaagcccgtc ctttagacag taattgtata
    25441 tgaccatctg tgttttcagg taggacattc ccaactgaat ctgtaatccg agcgcttgtc
    25501 atgtcttcca gtccaacgcc catatctctt gcatcacttg ttttcaaact ctctagtgta
    25561 tgagccttaa gaatcattgg tccgcattca ctttggccat atacttgaag aaataccgca
    25621 tcctgtgcca tcgacgttcg caaaaatgtc ttcattgtct ggttgtttat agcatcaaaa
    25681 gtggagtgat agtatcggat acccgaaaaa gcttctggat gctccttagc agtaaaacgc
    25741 cactgaacaa agttattcgg atgggtctct agtgcaattg gccggtgagc ttccaacata
    25801 tgaacaactt ttgaactttg tgcgtttgct aaaggcatca taggaaatcc catggacatc
    25861 aaagaagaga taccaatatt gaatctggaa tgcactggag agatgtgaaa agcgattaat
    25921 ttcttttccg aaatttttgt gaaaactgtt ttttgccatt tcgtccgcca tcccatagaa
    25981 tgagcagaat gacaaataag tttagggata ccagttgtac cagaagtatg ggtcatataa
    26041 gaaatttcat ttttgtctaa aaatgattga gaaacagaca aagcaggttg ttccattaaa
    26101 taaagaatag ga
//
LOCUS       DAZULG010000024        25720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000024 DAZULG010000000
VERSION     DAZULG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.024"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..2215
                     /locus_tag="ACG9CY_000965"
                     /pseudo
     CDS             156..2215
                     /locus_tag="ACG9CY_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
     gene            2298..2867
                     /locus_tag="ACG9CY_000966"
     CDS             2298..2867
                     /locus_tag="ACG9CY_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4937494.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3042..3848)
                     /locus_tag="ACG9CY_000967"
     CDS             complement(3042..3848)
                     /locus_tag="ACG9CY_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937495.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3986..5500
                     /locus_tag="ACG9CY_000968"
     CDS             3986..5500
                     /locus_tag="ACG9CY_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS4937496.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5903..6886)
                     /locus_tag="ACG9CY_000969"
     CDS             complement(5903..6886)
                     /locus_tag="ACG9CY_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS4937497.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6891..7538)
                     /locus_tag="ACG9CY_000970"
     CDS             complement(6891..7538)
                     /locus_tag="ACG9CY_000970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4937498.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7623..8255)
                     /locus_tag="ACG9CY_000971"
     CDS             complement(7623..8255)
                     /locus_tag="ACG9CY_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4937499.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8271..8822)
                     /gene="coaC"
                     /locus_tag="ACG9CY_000972"
     CDS             complement(8271..8822)
                     /gene="coaC"
                     /locus_tag="ACG9CY_000972"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS4937500.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8819..9556)
                     /locus_tag="ACG9CY_000973"
     CDS             complement(8819..9556)
                     /locus_tag="ACG9CY_000973"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS4937501.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9553..10542)
                     /locus_tag="ACG9CY_000974"
     CDS             complement(9553..10542)
                     /locus_tag="ACG9CY_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4937502.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10800..11342
                     /locus_tag="ACG9CY_000975"
     CDS             10800..11342
                     /locus_tag="ACG9CY_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4937503.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11359..12444
                     /locus_tag="ACG9CY_000976"
     CDS             11359..12444
                     /locus_tag="ACG9CY_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937504.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12456..13262
                     /locus_tag="ACG9CY_000977"
     CDS             12456..13262
                     /locus_tag="ACG9CY_000977"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4937505.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13263..14090
                     /locus_tag="ACG9CY_000978"
     CDS             13263..14090
                     /locus_tag="ACG9CY_000978"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4937506.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14087..15160
                     /locus_tag="ACG9CY_000979"
     CDS             14087..15160
                     /locus_tag="ACG9CY_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4937507.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15588..18284
                     /locus_tag="ACG9CY_000980"
     CDS             15588..18284
                     /locus_tag="ACG9CY_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4937508.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18349..18849
                     /locus_tag="ACG9CY_000981"
     CDS             18349..18849
                     /locus_tag="ACG9CY_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4937509.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18861..19355
                     /locus_tag="ACG9CY_000982"
     CDS             18861..19355
                     /locus_tag="ACG9CY_000982"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4937510.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19376..20185
                     /locus_tag="ACG9CY_000983"
     CDS             19376..20185
                     /locus_tag="ACG9CY_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4937511.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20182..21003
                     /locus_tag="ACG9CY_000984"
     CDS             20182..21003
                     /locus_tag="ACG9CY_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4937512.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21054..23153
                     /locus_tag="ACG9CY_000985"
     CDS             21054..23153
                     /locus_tag="ACG9CY_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS4937513.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23150..24268
                     /locus_tag="ACG9CY_000986"
     CDS             23150..24268
                     /locus_tag="ACG9CY_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4937514.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24307..25185
                     /locus_tag="ACG9CY_000987"
     CDS             24307..25185
                     /locus_tag="ACG9CY_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4937515.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25331..25528
                     /locus_tag="ACG9CY_000988"
     CDS             25331..25528
                     /locus_tag="ACG9CY_000988"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4937516.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            25512..>25720
                     /locus_tag="ACG9CY_000989"
     CDS             25512..>25720
                     /locus_tag="ACG9CY_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937517.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSI"
BASE COUNT         8133 a         4471 c         5429 g         7687 t
ORIGIN      
        1 cataagttat tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa
       61 tagtccatct tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt
      121 catgtttaga caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac
      181 aactaaataa acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg
      241 ttaagtttat gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac
      301 ccgttcaggc aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg
      361 attcccctat cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg
      421 cattgacttc ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc
      481 ctaacaaaac attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg
      541 atatgactga tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg
      601 ctttgacaga tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt
      661 attcacagtt tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg
      721 atctggtaga aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt
      781 caaacaatgg gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct
      841 attttgatcc aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg
      901 atcaaacagc tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta
      961 aagatcttgt tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca
     1021 aaatgaacgg gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata
     1081 tagactcgaa aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg
     1141 ctgcttcatc gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag
     1201 cagaaacaca tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt
     1261 ctgatcgagt gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta
     1321 tttctgattg gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg
     1381 aagtacttca ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta
     1441 cttatgttta tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa
     1501 acgaaaaaat aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg
     1561 taccgttaaa ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga
     1621 ccaaacaaac aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag
     1681 aactccatgc tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag
     1741 aacaaaaaag acaagaagaa agcttgtcag aaaaaaacaa caaatggctc atgcagtttc
     1801 tccaacaggg aaagtaaaac aaacaagaag cctagagtta gcaagaatat ctagaaaaat
     1861 ctcagaatct aataaaacga taaggcaaga agctcatttg gccccacaag tttctggatt
     1921 ggaaaatgaa attttagagc ctaaaataaa acaacaatca gaaaaagaat ttgttgctaa
     1981 acagtcggaa gatcagaagg aatcagatga ccagaaggaa acgaagaaaa aacgcttgtc
     2041 aactattgat tccaccgatg agcatctgca gccgcaagta ccacttccta gtccgggaac
     2101 acccgcacct ggtggaggta ttccttcttc tgctttagga gatttcatgc gaggtatggc
     2161 aggaacatgg gtatacgcta atcgaaatga atttgacgca gatgactggt tctgatttat
     2221 atctctatct gttatagtca aataagtagc tgagtcatat gttatttggc ataatggaga
     2281 cgaggaggga tagaaaaatg ttagataaaa cgttacctta cgcagaaatc tggatgacaa
     2341 gaccacttca acaaaatgtt ccaatcacgc ctttagcgga aggttttgaa tttcgatttt
     2401 atcaaaaaga agatgaatct gcttgggcgg agattgagac ttccgttggg gaatttcaaa
     2461 cgacagatga agcacttgag tattttcaac aaaactttgc tccttatcct gaagaacttg
     2521 aaagaaggat gttttttgta gagacaaaaa aaggagaaaa agtagcaacc tgtaccgctt
     2581 ggcgaaaaaa atcaagagag caagtgtatc cggtttttca ttggttagca gtgaaaccag
     2641 attaccaagg aaaagggctc gcaaaagcat tgactgccag agtcttgcaa gaatttccta
     2701 ccctccatac ggacggacca atctatcttc acacacagac atggagccat caggcgattg
     2761 ctctttataa aaaaatgggt tttactttta ttgctgaaaa tctagacggc acacctaatc
     2821 cagattatga aaaagttatg caggttttaa gtaaattaga taaataacga aatggtgaaa
     2881 taagtcctca gtattgggat aaaaaacgaa atagtcattc ctcatatctt acttttttga
     2941 gttaatacta tcggataact aatggcatgc catacattca aaaaagaagg ggcagtgaca
     3001 aaagtattgt cactgcccct tctcttgttt tttagcatgt tttatcgttt gattttttta
     3061 ggaataaaat tcttgatgaa cgtaaatttt tggtaatcgt ttcgactata gaggtaacca
     3121 aatacactga aaacaactgc tccaataaaa ttaacaaata aatccttcat agtatccatg
     3181 attcccacgt ctaagtaacc attttggatg attgttttat ctgttttacc atctgcattt
     3241 tgactttcaa tgattgtccg agtgatatct ggaacatgat agacagtgtt tccgtcttta
     3301 cttaactcca cactattgat ttgctggatg atcgtatctt tttgcatatc gaattgcagt
     3361 aatttatctg ctgaaaattc cacaaactcc cacataacac caactgtcat cgaaaaacag
     3421 aaggaaacaa ctgctagtaa taaaggagtc aagttcatgg tttttgtatt tttattgagc
     3481 aaataaacaa gggaaaagcc gacaccccca gctaaaaatc cgctgaatgc atgtagaaaa
     3541 gtatcccaca ttgaaaaata gccataaaaa tcacttaact ctcctaaaac tgttgaacta
     3601 aaaataaaag ataacaaaat gatttctagc atatttggaa tctgtatctt taatatcttt
     3661 tccagaaaat aagggatttc aaataaaatt aatgtcagta ataaaagcat tgtatttccc
     3721 cagtgttggt tccagacctg atccactagc ataataacga cgatgatacg taataaaata
     3781 tagattccgt ttgtgattct acctgcagtt tttgtactgt tcgtaaaaaa ggttttcaaa
     3841 tgattcataa aataactcct tcatgtatat ccttaattat actaagaagc gtgtgtttac
     3901 caaagctata tgacttaaag aaaaacagag ttgatattgt gaaaaaaatc acttaatata
     3961 gtataaggag aaaaggggga tacaaatgaa aaaattagtt gctggtttat taacagccgt
     4021 tgcacttctt gctatgagcg catgcggcgc agatcagaaa aatacagcgc agacaagttc
     4081 tagtacgaag gaatctgccg aagtatcatc aggcgcttct gaacaagtat atacagatcc
     4141 aaaagaaatg aaagaatctt atgatgtcgt gattattgga gctggtggtg cagggatggc
     4201 agcagctatc gaagcaaaag atgctgggtt gaatccagta attttggaaa agatgcctgt
     4261 tgccggagga aacacatcta aatcatcaag cggtatgaat gcttcagaaa caaaatttca
     4321 aaaagaagaa ggaatcaaag actcgaatga tgccttctat gaagaaacgc taaaaggtgg
     4381 tggaggaacg aatgataaag aaatgcttcg atactttgtc gatcactctg ctgatgcggt
     4441 cgattggcta gatcaaaacg gtatcaaact ggacaactta acgatcactg gagggatgag
     4501 tgaaaagagg acacatcgtc cgtctgatgg ctcggctatt ggtggatact tagtggaggg
     4561 gttattgaat aacgtccatg agcgggaaat ccctattttt gtcaacgcag atgtgacaga
     4621 cattaagaaa aatggggaaa cggtcaatga agtgactgta catgttcaag gagaagaacc
     4681 aaaagaggtc accggaaaag caatcgtagt taccacaggc ggttttggtg ccagcaaaga
     4741 atttattgaa aaatatcgtc ccgatttagc tgattatatt acgacaaatc aagaaggatc
     4801 aactggagat ggtatcacca tgattgaaaa attaggtgga caaaccgttg atatggataa
     4861 aatacaaatt catccaactg tgcagcagga tgagggggtc ttgattggtg aggttgttcg
     4921 cggagaagga gcaatcttgg tcgatcaaga ggggaaaaga tttgtcaatg aaatggatac
     4981 aagggataaa gtatctgcag caattacagc attgcctgaa aaatcagctt atttgatttt
     5041 tgaccaaggc gtgcgtgatc gtgccacagc tattgaattt tatgaccaaa aaggatatgt
     5101 cacaactgga aagacaatgg aagaattagc agaaaaaatc gatttaccaa aagaaacact
     5161 agcaaaaaca gttgatggat ggaatacgga tgttgcggag aaaaaagatg ctcaattcaa
     5221 ccgtgccaca gccatggaac atgacttatc gtctccgaat tattatgcga tcaaaatcgc
     5281 accaggaatc cattacacga tgggcggtgt gaaaatcaat acgaacactg aagtattaga
     5341 taaagaaaac cagccaatta gggggttata tgctgctggt gaagtcacag gtggcttaca
     5401 tggaaacaac cggatcggtg gaaattcagt tgcggagatc attatttttg ggcgacaagc
     5461 gggcatacaa gcagctcgat ttgcttcgga aaatgaatga aatagaacgt aaataaaaag
     5521 aggttgtgaa atcagcgttt tgagctaata ccgagggtca gctgattgaa cactgtttag
     5581 tgaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaacag
     5641 cttcttatgt ttttaacttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt
     5701 ttattcggaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     5761 cgtataaacg gcggaagcag aagaaattcc ttcggaaata agctgaaatt cacaaaaatt
     5821 tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc
     5881 ctcttttttg atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt
     5941 tgtttgcttc catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa
     6001 tgattggccg aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg
     6061 cgagctctgc tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac
     6121 gtttcccaat caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa
     6181 tcaagaccca acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc
     6241 cgatgaaatc caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt
     6301 gaaagtcttg ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga
     6361 gtttttcttc tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt
     6421 ggatcgctgc tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt
     6481 gtaattttcc aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag
     6541 cagcagaaac atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag
     6601 catattcggc ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag
     6661 tggagatatc acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt
     6721 agggaggttt caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg
     6781 tatggacttt gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt
     6841 ctaatacatc tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc
     6901 tttgcccatt cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc
     6961 gtacgagaaa tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga
     7021 gtgatcgttt ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca
     7081 tcgttagtca atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat
     7141 tcatctgcat aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt
     7201 tcatctgcat aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg
     7261 atcactgttt tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg
     7321 gcagcaaaaa tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa
     7381 gttaaatcaa aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca
     7441 tctctcaccc ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg
     7501 ccaatttgtc cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca
     7561 aatctttcca ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac
     7621 tatcagttag taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca
     7681 gtcgcaagac ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata
     7741 gctgaataaa cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa
     7801 aagtagatca tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag
     7861 aataaagaaa cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa
     7921 taaggaacca ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt
     7981 ccatgttctg ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg
     8041 ataactgtgc tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt
     8101 cttggtccta aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt
     8161 ggaccgatag ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac
     8221 atcgcagtta atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt
     8281 ctttcattaa gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct
     8341 tttccaaaat ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca
     8401 gatctcaatg tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg
     8461 gcaggggcaa tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga
     8521 tcgtctgcaa tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga
     8581 aacaagtcag cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc
     8641 atgacgtctg tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa
     8701 tgtgtgctgc ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt
     8761 gtaagatcag cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc
     8821 attttgattc ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt
     8881 gagcttcttc cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga
     8941 tttccgtcaa atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg
     9001 cgactgccag aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg
     9061 cttttggctg ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca
     9121 caagatgctc ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg
     9181 caaaccaatt gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt
     9241 cttctgaaaa actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat
     9301 cgtagtgttc ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga
     9361 tcgtataaat cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt
     9421 aatcgattgt ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc
     9481 ctgtcgaatg atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg
     9541 tgattagaat cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc
     9601 agcaagtagt ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag
     9661 ttcatctgca atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg
     9721 tacattgaca gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt
     9781 cgcgtttagt ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat
     9841 caatggaaga agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg
     9901 ctgttttcct aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa
     9961 agcacctaga ctttttagcc atgtgacaga ctcttctttt ctggaaaagg taatgattga
    10021 ctggaatcct agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc
    10081 agtaacgaca acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa
    10141 catagcagta aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg
    10201 tagaggaact aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt
    10261 gacgccaagc ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt
    10321 actttggacg acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac
    10381 ctgaccagac tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt
    10441 taccaagact tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa
    10501 aacaggttcc tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt
    10561 aaaattttct tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt
    10621 agacaaaaat taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa
    10681 gaaatattca gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact
    10741 aatacgagtg gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta
    10801 tggaaattgg tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac
    10861 taggcgaacg cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt
    10921 caccatcaat ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat
    10981 tttttcatca agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt
    11041 atttcgatga agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag
    11101 aaatggagcc tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt
    11161 cattatctga aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga
    11221 cggtctatgt ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat
    11281 taaggaatca tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt
    11341 aatatagggg gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc
    11401 tatgatgatg aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat
    11461 acactgcttg gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc
    11521 accgaagcta cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct
    11581 aataagcgac aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt
    11641 tttgataacg ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag
    11701 aaggtaaaag atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt
    11761 gaaatgtctg gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg
    11821 aaagtattgc tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg
    11881 cagtatgaac tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat
    11941 gaccaagaag aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc
    12001 gtccaaagtg gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat
    12061 tttgttgggg agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt
    12121 gttggtaagc aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt
    12181 gttttacgcc cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg
    12241 gtcgatactc agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa
    12301 aatgaatgga tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc
    12361 tttgagccag aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca
    12421 cgtcttgaca gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt
    12481 ccgtccctta tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt
    12541 atcaatcttt ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta
    12601 cgtcggggac atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt
    12661 ttttcacgct gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc
    12721 aattatggct gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg
    12781 catttattgg gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga
    12841 tcgggccgaa acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg
    12901 agattccatt tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga
    12961 tcagtgctag tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc
    13021 cgttatcatt aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt
    13081 tattcatgct gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg
    13141 aacagcattt cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa
    13201 tcattgctat gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat
    13261 aaatgaaaaa atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg
    13321 caccgatctt ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta
    13381 caggatttac ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg
    13441 tattgaatac gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat
    13501 ttggggctat gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca
    13561 tgaataacat cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct
    13621 ttacactact aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca
    13681 gcattccaat cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg
    13741 tcgatgcggc tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc
    13801 cttatctttc tccaggaatc atagctggat attttatggc atttacctat tctcttgatg
    13861 attttgcggt tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct
    13921 attctcgtgc aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct
    13981 tgttctccat gatattggtc attggatact actttattag taaagaaaac cgtccaaaac
    14041 ggctgaagaa aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca
    14101 atctctgttt ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact
    14161 tgaaaaaaca agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta
    14221 tatcgatcct agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga
    14281 aacatttgat tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga
    14341 catcgctata ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc
    14401 attagatcat tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga
    14461 ttttgatcca gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata
    14521 taacgataaa ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga
    14581 attaaaagac agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa
    14641 tagtttaggc tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa
    14701 attgaatcat ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat
    14761 gatcaatgaa gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg
    14821 ggaaaatgaa catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa
    14881 tatcgttatg ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat
    14941 gctagaaccg gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga
    15001 agaagcaaaa gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga
    15061 tgacacgatc tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta
    15121 taatgaccta ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat
    15181 ggggacttat caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa
    15241 tcttctacca aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac
    15301 gtttttctac cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag
    15361 taaatctaag attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac
    15421 ttaacccttc tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc
    15481 tatgaaaaaa cataaaaatc atattagata aaaactaaat tggtagataa attattttgt
    15541 cagatatctg aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat
    15601 atagaataga catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg
    15661 aaaaaatgaa aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga
    15721 atatgtcttc tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg
    15781 ccagccatta tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc
    15841 ctgttttgtg gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg
    15901 aagcctttca ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta
    15961 tagccgctgt caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg
    16021 ttggaaaaag ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg
    16081 aaaaagatgc accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc
    16141 tttcggcaac actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg
    16201 atggtttatt ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat
    16261 cgaaagaaaa tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg
    16321 gtgaaaatgt aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag
    16381 gtgaaaatta tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa
    16441 cttttgaaga tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg
    16501 aagcggatgt tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat
    16561 ctcatccctt tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg
    16621 atgtgtatag tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc
    16681 agttaaatat gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga
    16741 aaaatcaaaa gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa
    16801 aaattactca gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg
    16861 aagtacagca tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat
    16921 tgatgattca gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga
    16981 cgacgagtgg acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata
    17041 agacagactt aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact
    17101 tatgtaatca gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa
    17161 gactgcttat tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc
    17221 tgttgcttgc tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat
    17281 gcggcaatta tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg
    17341 tcgaatcgac aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg
    17401 tagatatggg ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt
    17461 tacttcttct gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga
    17521 tgaatgcgcc atttaaacag attgcggagg atgcagcaga caagtattca atcaatgttc
    17581 aatactttga aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg
    17641 ggatttcgga caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag
    17701 ttttcacgaa ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt
    17761 ttgacaagac aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta
    17821 atattaatgt ttatgacatc ttagacggtg atggagcagc caatttggag catattttga
    17881 gtcctgaaat gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt
    17941 cgttggaagg aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag
    18001 tggaaacagt catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa
    18061 aactaaatct ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag
    18121 aaaaatctga agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt
    18181 ctagaagtat ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc
    18241 tttcgctgct ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg
    18301 tctaatctgt tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca
    18361 atgtttttaa aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt
    18421 aaccatatga agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc
    18481 agtattcaat tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat
    18541 gagtgtgatt ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag
    18601 atttttgttt ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt
    18661 ttggagaaag agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa
    18721 atagtattgg caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc
    18781 caacaggaat taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat
    18841 tttttttagg aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg
    18901 gacaagtagc gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg
    18961 atgaggcggc taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg
    19021 tcaaagtcat tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga
    19081 aggacatgcg cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag
    19141 ctttcccaac ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat
    19201 cggaagataa gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact
    19261 taaaagaatt agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa
    19321 aacgttcagc tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta
    19381 tgatgcattt gtagtcgctt tggccgtttt tattggagtt gctggacatg aaatgttcgg
    19441 gatgtccatg ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga
    19501 tttgcagacg gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa
    19561 tatcggaatc tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc
    19621 aatcaaaatg ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt
    19681 aggattacag ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt
    19741 tttcaaattt gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg
    19801 gtttgtccac tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga
    19861 tgttgtcaaa agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc
    19921 tggaaatctg ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa
    19981 tattgctgtg tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat
    20041 tgcaattgcc gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact
    20101 aaaaaatata tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca
    20161 agaagaggag gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa
    20221 ttggttcgga agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg
    20281 atgggcggcg gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt
    20341 gaagaagatc gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct
    20401 tgcgcgtcct ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct
    20461 ggttttgacg tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga
    20521 attggcgatt cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac
    20581 ttagccaatg ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct
    20641 aattacttga tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt
    20701 gaaaagctgt atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt
    20761 ctgatcatgg taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt
    20821 tcaatgaagg gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga
    20881 attgtaccgt tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc
    20941 atcaaaatta tttttggaat ggtcgtcttg ggaattttat tgtcattact tggaatcgca
    21001 tagtaggaag ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta
    21061 ttaatgaagt aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa
    21121 atgtgactac ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga
    21181 ccttcgatgt aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa
    21241 cgttgacctt ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata
    21301 aacagctctc tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc
    21361 atttgtcaga atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat
    21421 atattcatca ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta
    21481 ataagcgttt aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc
    21541 ttcaagtttc agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg
    21601 tgttgatttc tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg
    21661 ccattgcttt tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata
    21721 tcgaaaaatg gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa
    21781 tgccaaacga tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt
    21841 tcggtatgaa tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta
    21901 atcgaatttg gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg
    21961 agttatgtca aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa
    22021 aatttacagg aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta
    22081 gtctttattt tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac
    22141 tgaatgcttc aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc
    22201 tttttccatc tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca
    22261 atctttgtct ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac
    22321 tgagtcttag caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac
    22381 aaatgactat cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga
    22441 aaacaaccta ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt
    22501 taagtgatgt gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag
    22561 acactacgtt gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca
    22621 ccatggcctt ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca
    22681 cttggggatt agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc
    22741 tgcacaaccc tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag
    22801 acggttcgtg gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag
    22861 actttggctg cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc
    22921 aatatttcgg attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa
    22981 cgttttcttg gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat
    23041 ccgagttgag tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa
    23101 ttcttcacgt agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact
    23161 aaaaaatatt acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac
    23221 aggcgagaaa ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg
    23281 tggcgcaatt ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg
    23341 aataattcgt catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt
    23401 acgtgattta atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc
    23461 tttaggggat ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat
    23521 agtggttaat cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc
    23581 taaaattacg atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa
    23641 ttacgctttg gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg
    23701 ggaacgcatt accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg
    23761 ctattacgtg ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg
    23821 cgagtttaaa ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc
    23881 agaagattat tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca
    23941 tgcgaaaaac tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac
    24001 aagagatcgt tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat
    24061 gccacgacat ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt
    24121 atcggtcact ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga
    24181 tgttgctagt gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt
    24241 cactgatcga gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa
    24301 cctatgatga tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt
    24361 actgaggaat taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca
    24421 atgaagcagg tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc
    24481 ggctgctttg gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca
    24541 acaccaaaac ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat
    24601 attccgattg aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt
    24661 tctggcaaag ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt
    24721 gccttgttca atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg
    24781 atcaagcgac atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta
    24841 gaggggatgg cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta
    24901 ttggctgacg attcgttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat
    24961 acaacactga tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa
    25021 cacatgaaga aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata
    25081 ccaccaattg atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc
    25141 ggattagcat tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca
    25201 ttgatactca tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa
    25261 agcgaaaatg aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca
    25321 ctgaaaggaa atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag
    25381 gatattcact ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt
    25441 aatagttata gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct
    25501 tggcttaatg tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta
    25561 attctattat agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta
    25621 tacattttaa atattttatt cctatttcta ttgctaaaaa agtagaaatt ctatatttac
    25681 ctttaactct cgcttctctt gcaaatgtag ttagcatttt
//
LOCUS       DAZULG010000025        25680 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000025 DAZULG010000000
VERSION     DAZULG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.025"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            279..1127
                     /locus_tag="ACG9CY_000990"
     CDS             279..1127
                     /locus_tag="ACG9CY_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS4937518.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="ACG9CY_000991"
     CDS             complement(1298..1639)
                     /locus_tag="ACG9CY_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFS4937519.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="ACG9CY_000992"
     CDS             1725..2432
                     /locus_tag="ACG9CY_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4937520.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="ACG9CY_000993"
     CDS             2419..2742
                     /locus_tag="ACG9CY_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4937521.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="ACG9CY_000994"
     CDS             complement(2831..3868)
                     /locus_tag="ACG9CY_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4937522.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="ACG9CY_000995"
     CDS             complement(4315..5298)
                     /locus_tag="ACG9CY_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS4937523.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5714..6484)
                     /locus_tag="ACG9CY_000996"
     CDS             complement(5714..6484)
                     /locus_tag="ACG9CY_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4937524.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6477..7412)
                     /locus_tag="ACG9CY_000997"
     CDS             complement(6477..7412)
                     /locus_tag="ACG9CY_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937525.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8006..9655)
                     /locus_tag="ACG9CY_000998"
     CDS             complement(8006..9655)
                     /locus_tag="ACG9CY_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4937526.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9850..10746)
                     /locus_tag="ACG9CY_000999"
     CDS             complement(9850..10746)
                     /locus_tag="ACG9CY_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4937527.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10739..11419)
                     /locus_tag="ACG9CY_001000"
     CDS             complement(10739..11419)
                     /locus_tag="ACG9CY_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4937528.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11420..12202)
                     /locus_tag="ACG9CY_001001"
     CDS             complement(11420..12202)
                     /locus_tag="ACG9CY_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4937529.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12195..13112)
                     /locus_tag="ACG9CY_001002"
     CDS             complement(12195..13112)
                     /locus_tag="ACG9CY_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4937530.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13115..13399)
                     /locus_tag="ACG9CY_001003"
     CDS             complement(13115..13399)
                     /locus_tag="ACG9CY_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937531.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            14187..17414
                     /gene="fss3"
                     /locus_tag="ACG9CY_001004"
     CDS             14187..17414
                     /gene="fss3"
                     /locus_tag="ACG9CY_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS4937532.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            17526..17840
                     /locus_tag="ACG9CY_001005"
     CDS             17526..17840
                     /locus_tag="ACG9CY_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4937533.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17853..18227
                     /locus_tag="ACG9CY_001006"
     CDS             17853..18227
                     /locus_tag="ACG9CY_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4937534.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            18237..18581
                     /locus_tag="ACG9CY_001007"
     CDS             18237..18581
                     /locus_tag="ACG9CY_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS4937535.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            18650..19666
                     /locus_tag="ACG9CY_001008"
     CDS             18650..19666
                     /locus_tag="ACG9CY_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4937536.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            20260..22134
                     /gene="ltrA"
                     /locus_tag="ACG9CY_001009"
     CDS             20260..22134
                     /gene="ltrA"
                     /locus_tag="ACG9CY_001009"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4937537.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            22236..22616
                     /locus_tag="ACG9CY_001010"
                     /pseudo
     CDS             22236..22616
                     /locus_tag="ACG9CY_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            22661..23803
                     /gene="dcm"
                     /locus_tag="ACG9CY_001011"
     CDS             22661..23803
                     /gene="dcm"
                     /locus_tag="ACG9CY_001011"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFS4937538.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            23793..24082
                     /locus_tag="ACG9CY_001012"
                     /pseudo
     CDS             23793..24082
                     /locus_tag="ACG9CY_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24338..25522
                     /gene="mobT"
                     /locus_tag="ACG9CY_001013"
     CDS             24338..25522
                     /gene="mobT"
                     /locus_tag="ACG9CY_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4937539.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            25519..25632
                     /locus_tag="ACG9CY_001014"
     CDS             25519..25632
                     /locus_tag="ACG9CY_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4937540.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         8057 a         4994 c         5032 g         7597 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa
     5521 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     5581 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac
     5641 aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt
     5701 cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg
     5761 cgtacaaaac aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga
     5821 tcgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg
     5881 aaacagcttg ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag
     5941 ttaacggctg caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa
     6001 tcccgttgag cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga
     6061 ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag
     6121 agccgacgag aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg
     6181 taggaacagg tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg
     6241 acgaagaaac attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga
     6301 tgaattccat atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga
     6361 aaataacaaa atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac
     6421 gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa
     6481 acatttttct cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa
     6541 cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag
     6601 attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat
     6661 gtacaagcat atccttcaat cacaggtttg gtatcatatg gcgctaaatc aaagataaat
     6721 gtttcaaatt gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc
     6781 gattggatga ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc
     6841 aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc
     6901 aactcgatgt caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc
     6961 aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca
     7021 tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct
     7081 ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa
     7141 gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc
     7201 ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta
     7261 ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct
     7321 ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt
     7381 ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat
     7441 tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca
     7501 cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact
     7561 atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga
     7621 catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc
     7681 cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag
     7741 ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg
     7801 tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt
     7861 attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat
     7921 tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc
     7981 acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga
     8041 acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca
     8101 gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga
     8161 ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa
     8221 tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc
     8281 accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct
     8341 catctacgaa gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg
     8401 atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg
     8461 aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct
     8521 gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg
     8581 aacccgtgac taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca
     8641 atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga
     8701 tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt
     8761 cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg
     8821 agataaatgg cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc
     8881 cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct
     8941 gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg
     9001 ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat
     9061 aatagaccgt attgaatact aagccaccga ttgctagata gattcctagt acttgcccta
     9121 aaagaagcaa acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc
     9181 gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt
     9241 ttcctaaacg agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa
     9301 aagcaattag tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat
     9361 acaacgcttc tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca
     9421 tatccaagtc ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct
     9481 ccgtgagcca cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt
     9541 ttccccaatt actacgaact gtttccttta ctttcaaacg tgaaaaaatg tctaaaacaa
     9601 ctaataaaat aacaactgca ataataatta aaacgataaa ttctctttgg cccatatccc
     9661 tctccgttcc ttgatttgtt agttttagta tagcactgtt gcgctatttg aaaagggggt
     9721 acaaaaataa ctgtcttcgt tacctaattc gtatcaatca caccaaaata aaaacacctt
     9781 ccaaaaaaga agatgtcttt aaaacgagat acaatttttt gtcattcact tcaggtaaat
     9841 tagagccaat taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct
     9901 agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc
     9961 gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc
    10021 aacagaggtt tgcgctcctg gtttgatagg attggccact gttaatcgaa tgaatgaatc
    10081 ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat
    10141 attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg
    10201 ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac
    10261 ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc
    10321 tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt
    10381 gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac
    10441 ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag
    10501 tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaccca
    10561 tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg
    10621 accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc
    10681 cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca acagccactt
    10741 aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta
    10801 gtttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt
    10861 cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa
    10921 aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta
    10981 catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt
    11041 ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaac agttcttcga
    11101 tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc
    11161 tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg
    11221 ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag
    11281 gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc
    11341 tctgttcaag aacctcctcc agtaaagaat gaacagcttc atcgtcttcg ataatcaaaa
    11401 tagtttccag caggaacacc tacttaatgt cagatttttg gaaaataaac attcctagag
    11461 ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag
    11521 ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa
    11581 attccaagcc tttgattttt gcgaaaacac cgggaacgaa ggccgttccc atgatataaa
    11641 gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa
    11701 cagataatgt cgctaatatc agcagaagca atacaggaaa tacttttaga acataatttc
    11761 catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaatcatgg
    11821 ctagccccgt aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg
    11881 acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt
    11941 gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag
    12001 ccattttttc caccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt
    12061 ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa
    12121 gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt
    12181 cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc
    12241 agtttttgcc caactaggtg gataccatcc acaggaactt ttgcctctac caatgcttga
    12301 ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct agattggaca
    12361 aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg
    12421 tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc
    12481 aagtgtcctt catgcaaaat accgtagtga gtggcgatct gtgacaattc atctagcaag
    12541 tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc
    12601 atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct
    12661 ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc
    12721 tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct
    12781 ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct
    12841 ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc
    12901 gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc
    12961 ataaaggtag acttacccgc tccattctcc ccaatcaaac catagatcat tccacgtttg
    13021 atttcgatcg atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt
    13081 gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgataagtg
    13141 aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt
    13201 ccaattgaat ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt gtctgccaat
    13261 caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta
    13321 tcgttgacat cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat
    13381 aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt
    13441 ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct
    13501 tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca
    13561 aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca
    13621 caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt
    13681 tatagatcga atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa
    13741 aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct
    13801 cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt
    13861 aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggtttt ttcttcgtat
    13921 cattgaataa gtttgaaaag ccttgattta taaaggtttt tccgtaaata tattttgtta
    13981 tatttgatta aatttgaaat gtttgtgttc taaaatgtgt tctaatttaa aggcagtata
    14041 tttcgtattt tatattaaat ttttaggctc attcattctc aaatccattc tatttagtca
    14101 ataaatattt taaaaagact ttataaacaa agatgttcaa gcgagcatct tatttttttg
    14161 tgcctaattt gaaaggagaa catttcatgt ataaaaaaca aaaaaagaaa ttatgtgtat
    14221 taactatttt gagtttggct gttcaatcaa ttgtaggagc aattgctcct acgattgctt
    14281 ttgctgatga aattacccat ccacaaacgg taaccgtaga actagattta gcacatcaat
    14341 atgctgttga aggaactttt agtgatggtc gtccaatgtc agaagtcact gttccgcatt
    14401 atgcagttta caatggtgtg aagcaagata ttttctgtgt tgaaccagga gtcccgattt
    14461 acaatgagtt cactcctgga tatgagaaga acccgttacc agatatgtcc gataaagcca
    14521 aattagtttc cgttttatgg aaaaaagtag gtacagatgc agatactcat attgtggcac
    14581 agaagatgat ctggcaagaa gttaatgggt atacccttca ctcaataaaa cgatcagatg
    14641 gtagtgccgt aaatatcgca gccattgagg caaaaatcaa tcaagccatt gccgattatc
    14701 agaagaaacc aagcttccat aatagtacgg ctaaaacagt attagggcaa tcgactactg
    14761 tgacggatac gaataatctg aatttgtcag agttcgatga ggttgtggaa aataccgcaa
    14821 acattgatta tcgtgtgaat ggcaatcagt tgataatcag cccaaatgcc aattcaaaag
    14881 aaagtggtgt gttgacgctt aagaaatctg ctggtaccgg aacacctgtc gcctataaaa
    14941 aggctggcca acagacttta atggccgggg cgatcgataa gccgaacact tatacggtca
    15001 aaattgatgt ggaaatggag ggttctttga agattaaaaa agttgataaa gaatcgggtg
    15061 atattatacc aggaacggtt ttccatttag actttggaaa aactttaccc gcaaaagacg
    15121 tgaccaccga taaagaaggc attgcgacct tagatgagat tcctcatggt acaaaggtaa
    15181 ccattactga aaaatcggtg ccggcgcctt atacgattga taccacaccc atgactgcca
    15241 caattaaagc aggtgaaacc atttcagtga cttcaaaaaa tacgcgagaa aaaggtcaaa
    15301 ttattctgga taaaaccgga attgaaacag ggagtgatct ttggaacgat aattactctt
    15361 tagcgggcaa tacgtttgcc attcgcaagg atagcccaac tggtgaaatc gtccaagaaa
    15421 tgaccacgga tgaaaaaggt catgcagaaa caccaaaaga gattgccaat gcgttggaat
    15481 tgggaaccta ctatgtgact gaaaccaagg ctagtcatgg ctttgtgaat actttcaaac
    15541 cagtaaaagt cgaattgaat tatgtcaatc aaacagtggc tcttgttacc agtaacgtaa
    15601 aagggcaaaa ccaagaaatt actggtgaaa ccactttgac aaaagaagac aaagataccg
    15661 gtcatgaaac tcaagggaaa gctgagttta aaagagctga atacaccttg tttactgtta
    15721 aggatggaaa agcggtcaaa tggaccgaga actttaaacc agaattgttg aagggaacga
    15781 aagcttctga tgaaaccgta actttgactt tagatgaaaa gaatcaagtt gccgttaaac
    15841 acttagcgat taatgagtat tactggcaag aaaccaaagc gcctgaagga tataccttgg
    15901 atgaaacaaa gtatcctgta tccatcaaaa aggtggatga tagcgaaaaa aatgctgtga
    15961 ttactcgaga tgttgcggca aaagaacaag tgattcgctt tggctttgat ttctttaaat
    16021 ttgctggatc aactactggc actgccgaaa ctggcttcaa tgatctaacc tttaaagtgt
    16081 cgccattgga aggaaccaat gaaatcaccg gtgccgaaga tgaagcgacc acagcttata
    16141 acgagcaatt agggtttgat ggttatggca agtttgaaaa tcttccttat ggggattatt
    16201 tacttgaaga agtagaagcc ccagaaggtt ttcaaaagat tacgccatta gaaatccgtt
    16261 ctacttttaa agaaaataaa gaggattata ccaagagtga ttatatcttt actattaccg
    16321 aaaaagggca aaagcaaccg attaaaacgg taacggtccc ttatgggaat ctaaccaatg
    16381 agaatttctc agtcagtttg aaccgtttga tgctttatga tttgcctgag gaagaagata
    16441 gcttaacttc tcttgcgact tggaaagaag gagataaaga attgactact cttgattcta
    16501 ccgagctagt cgataaattg agctataatc tacatgaaat caaagaagac tggtatatcg
    16561 tagctcaagc catcgatgta gatgctacaa aagctgccca agaaaaagat aaaaaagcca
    16621 aaccggtggc gattgcggaa acaaccgcaa ctttagcgaa taaagagaaa acaggaactt
    16681 gggagattct gcataaatta actgtcgaac aagttttaga caagaccatt gtcttattca
    16741 actatgtgta tgaaagcaaa gaagcctatg aagctggtga tgagccggta gcgaaggatg
    16801 ctagcttgaa caatcaagcg caaactgtta attgtatggt ggaacgtcat gtttccatcc
    16861 aaaccaaagc gcacttagaa gaaggttctc agacctttac tcatggtgac gtggtagata
    16921 tgtttgatga tgtatccatt acccatgatg tactagatgg ctcaaaagaa gcttttgaaa
    16981 caattctgta cgctttattt ccagatggta caagtaaaga aatttggaaa tctggcaaga
    17041 ttgattacga agtgaatgac aaagaattta ccaaaaccgt acttgcggaa aaagtggata
    17101 caagtaaata tccagaaggt acaagtttta ccttcaaaga aattaactat gacaaagatg
    17161 ggaatatcaa tggaaaacac aatgaagatt tgaaagaaaa atctcaaact ttaacgccca
    17221 aaaaagttcc gacaacacca agtacaccag aacaatctga aatgccaact gttccaagtg
    17281 gctctcaaga atctagtcct acagtgaagg cattccctca aaccggggag aaaacttcca
    17341 atgtgctact gttcattggc tttagcttga tctttgcgac ggctggctat tatttttgga
    17401 atcgtcggaa ctaaggtgat aagatgaaac atgtaaatag tagaaaacaa tacgcgcgac
    17461 cgcctcctaa aaaagtggtc gcgctatttt tgtattcaaa actaacaatg aaaagaggaa
    17521 aaaatatgga attgaaattt gttgtaccag atatggtcga gacgttcgga aaactaacat
    17581 acggtggtga aggtgaagtt ctatcggaag ggtatggtcg aaatactaca attgttggtc
    17641 gaagttacca tctgttttca aataaacaac gggccgatga tattgaagtg gtggtagctg
    17701 cggaagccgg cgaaaaggat tttgaccaag atcaaccact aaaagctgta aatccccact
    17761 taattgccaa aggctatgaa attgagaatc gtgggtttac cgattacgtg ttgtatgttg
    17821 atgatttagt gaaagcatag gaggaaaaga agatgagatt aacagaaggt attgtcgtag
    17881 attcaggatt aacctttggc aagttgcgtt tttccgcatt acgtcgagaa gtacgcaagc
    17941 aaaatgagga cggaacggtt agtaacgagg taaaagaacg gacctataat ttgaaatcct
    18001 ccgcgcaagg tcgaatgatc caagtcagta ttccagcaaa tgtaccctta cgcgagtttg
    18061 cttatgatgc agaggtagag ctggtcaatc caatcgtgga tacggtcgcc aattatgttt
    18121 ttcgggaagg aaccaccgtt aattggttta tcaaggcaga tgatttagtc ttgaaacgac
    18181 aactgaatca aggaaaacct acgaaccaaa atgaaggaaa gaaataggtg aagaatatgg
    18241 aaattaatgt tgaacatatt ttagattgtc tcgatcaatt tggcaaaggt gagctaacgg
    18301 aggaacaact cactaacgca ttaacctatg atgaaaaaat gtttctgatt atgcatcaag
    18361 ggttactgga agtaggtaac gatgccaaag aagactttga tgtactggat tggttagggg
    18421 aagaagagtc tttctttatg gttgtcgagg taaatgaaag tttatgtcgg caggcagaat
    18481 ctgttttgga agaaatcgat gtggagatgc cggatgccat cgaaagattt ttaaaacaat
    18541 tagtggaaac gaaacaactt cccgtagtag taaatgacta gcataacttt ttgaaattgc
    18601 ccaatgaaat cctattgggc tttttcgact ggaggtggaa accatcaaaa tgtacaaagg
    18661 acatcgcata cgcgcaggag atcaacactt gatttatcac tttgttcttg gctggctaat
    18721 ctttttgttc attggttgga tgagcgtctt ttatttacaa gaacttagac aatttgatat
    18781 ttctaaggta tcattttcca ctagtgaaat tggctggtct atgaaggatg taatctgctt
    18841 gctaggaagt ctcgctcttt caggaagcat gatgttactt tatatacact tttttcagga
    18901 tcattggcga agtctttggc atcgacaaaa gttagctcgg atgattctag aaaaccattg
    18961 gtatgaagta aagcagactc aaggtgaagg ttttttcaaa gatttgaata gtagttggac
    19021 caaagagagt atcagttact tccccaaaat ttattatcga atgaaagatg gcttactttc
    19081 tattcgcgtt caaatttcac tgggaaaata tcaggatcag ctattgaagt tggaaaataa
    19141 gttagaaagc ggactgtatt gtgagttagt agaaaaagaa ctcaaagact cctatgtgga
    19201 atacaccttg ttatacgata tgattgccaa tcgaattggg atagaagaaa tagtggcaga
    19261 aaatggggcg ttgcgattga tgaaaaatca agtatgggcg tatgattcct tgccgcatat
    19321 gttaattgct ggcggcacag gtggtgggaa gacctatttc ttactcacca ttattcaagc
    19381 actattgaag tcagatgcag aattgtttat cctcgatccc aaaaatgcgg acctcgccga
    19441 tttaggtacg ataatgcctc acgtatactc ccaaaaagaa gaaatttctg aatgtgtgga
    19501 agatttttac gaacgcatga tagctcgtag taggtcaatg aaagaaatgc ccaactacaa
    19561 aacaggagag aattatgcgt atctcggact tccgcccaac tttttaatct ttgatgaata
    19621 cgtggcttat atgggtgcga atcgttttcc tacaagcatc gaatagatgt gaaatggaag
    19681 cctattgaga ttatcaatag tgacaactaa tgttttgaga gatggaatga aagccttcaa
    19741 gttgagctga aacatccccg ctaatactcc tacatgctct aatcaatgaa gtagattggt
    19801 tttggtatga agctaggtga agtcgtctga aatctgccta agtgcttgag cataaggctg
    19861 actatagggc gggcggctga aaaagataac tatggtaaga atgtctgaaa atgactgacg
    19921 aatctgcgaa tgtacgggtc taaaaggctg atcttattga aagataggaa catacggacg
    19981 tatggtgaga taccgacaag taaggttcca tgatatgaaa atcgggatag ctaacgaaga
    20041 agctataatt tcacaggctc atagtagata cctaaggtta tacatgatag ctaaaaacat
    20101 tggaacgatg taagcagaaa actatcaatt aaatgactac ttctagatag gtgcatataa
    20161 gtaataacga aattgctttg tttctgtgaa agtaagggca cagtaccgat gaagcgtcta
    20221 acaaatgtgg agggatagcc ctaagtctat ttaataacaa tgaatgttga aacagatgaa
    20281 gttcaaggat ctggtaagaa cgtgggaaaa tccgttatgg aggtgccacg cttgaacaca
    20341 aaaatacgtt actgggaata ttatgatcta caagaaacat tcgatcagct atttactcaa
    20401 agtagaaacg ggaaaaagtt ttatcaatta tatgagttaa tcatttctga aaataatatt
    20461 cttttagcat accgtactat caaggcaaac aaggggtctt cgacaccagg aacggattcc
    20521 ttcacaattg ataactataa ggaaatgaac caagcagagt ttatccatct tattctcagt
    20581 catttagaaa attacaaacc aaaatccata aagcgagtaa tgatacctaa acccaatggt
    20641 gagaaacgcc cattaggaat tccatgcatg attgatcgaa tgatccaaca gatgttcaag
    20701 cagatactgg agccaatatg tgaagctaag ttttatgaac atagctacgg tttcagacca
    20761 ttgagaagtg caaagcatgc tctaggtcgc attatgtatc tcataaacat cagtaaaatg
    20821 cactatgcag tagacattga tatcaaaggt ttctttgata atgtaaacca ccgtttactg
    20881 ataaaacaat tatggaatat aggaatttgt gacaaacaag tcttagctat tctttcaaaa
    20941 tctctgaaat cacccattca aggagaaggt atcccgagca aaggaacgat ccaaggaggt
    21001 atcatttctc cactattatc taatgttgtt ctaaacgact tagaccattg ggtctcaaag
    21061 cagtggcata cctttgaaac aaagtatccc tatactaaag gttacaataa atttcgtgct
    21121 ctaagagata caaatctaaa gcaaggctat attgtcaggt acgcagatga ctttaaaatc
    21181 atgactaatg attatccgac agcattaaaa tggttccacg ctgtcaagct ttacctaaaa
    21241 gacagactta aattagatat ttccaatgag aagtctaaaa tcgtcaattt gcgtaaatgt
    21301 aagtcggaat ttttaggatt cgctatttgc gtgaaacaaa aagggaaaaa atgggtttgt
    21361 aattcacgta tttctaacaa aaaaaaggat cagattaaag aagagattaa acaaagaatc
    21421 aaagatattc aaaagagtcc tacagcacaa aatgctctcc tgtttaatgc tctcgtgctc
    21481 ggtgttcata attatttcag gtatgccact catattaatt tagatttgaa taggatgaac
    21541 tacgttcttt cattcactac ttacaaccga ttatcaaatt gtagtacaag atgttacccc
    21601 aacaaagctt ccccaactta caaaaagttt tactcaacaa cgatgaaaac gtataaagta
    21661 gctggagtct atttgtaccc tctttgcgac gtatctacaa aaacaattta cggatttaat
    21721 acggaggata caccgtttac tccgtttggt cgtcaaagat tggagcacaa aagcctacag
    21781 tctcttgtgt tccaagaatt aagaaaattg atggaatcga aaataccgaa tagaacggtt
    21841 gagtatttag atagtagaat atctcgttac tcaatgaaat caggcaagtg tgagattaca
    21901 aaacaatttt tgccagcaaa agccgttcac tgtcatcact atttgccaaa aagtcttggt
    21961 ggagacgaca aattcgataa cttgagaatt attcataagg atatccatct tctgatccat
    22021 acaacaaata aaatgataat cgatcactac gtgcatgaac tgaaactggt gccagagcaa
    22081 atagcaaaaa tcaaccttta tcgaaagatg tgtaatctgc aaaacattca atagaaaata
    22141 aatagatgga acgccgtatg aggtgaaagt ctcacgtacg gtgtggagcg ggggaaaaga
    22201 tggagattat atcaaattct tacctatcgc tatagatgtt aacgacaaaa gaaagtgcgg
    22261 tgattttgaa taagcttaaa caaatcgtga tgttaggccg tcaatctggt ttctttctga
    22321 ttctagcttg tcaaaggccg gatgcgaaat atctaggaga tgggattcgc gatcagttta
    22381 acttccgtgt ggctttgggt cgtatgagtg agcttgggta ttccatgatg tttggagaag
    22441 tcgataaaaa tttttttatg aaacgcatca aaggacgagg ttatgtagat acagggggca
    22501 gtgtgattag tgagttttat acgccacttg taccaaaagg gtatgatttc ttaagggaaa
    22561 ttagcaacat tgttggatta accgttcata cggaaagaga aaataatagt gtgtagccta
    22621 ggaatatcaa taatcctggg cttttttatt gaggtgattt atgacatttt tagatttatt
    22681 cgccgggatt ggtggctttc gcttaggtat ggaacaagcc ggtcaccagt gtatcggttt
    22741 ttgtgaaatt gatgaatttg ctcggaggag ttataaagca atccatgata caaggaagga
    22801 ggtggaaatg catgacatca caagtgtatc agacgaattt gttcgatccc tcggaccagt
    22861 ggacatcctt tgcggcggct ttccgtgcca agctttttca attgcgggaa agcggcaagg
    22921 attttccgat actcgaggta ctttattctt tgaaatcgct cggttcgcca ctcttctcca
    22981 acctaagttt ctattccttg agaacgtccg gggattgctc aatcacgaag gaggggctac
    23041 gttcgagacg atcctccgaa cgttggatgg attggggtat gatgtggaat ggcaagtgct
    23101 taactcaaag gcctacgttc cccaaagccg tgagcggatc ttccttatcg gacattctcg
    23161 agatgcatgt accgaacaag tatttcctat cattggatcg tcttcaacat ctgatcaaaa
    23221 catcagaaac ttggtaaata tcaatccttc caatcgagga atgggtggcc aagtctatgg
    23281 ttcagatagc gtagcaccaa ccttgaccgg tgatgaagga attaaaattg ccttaccggt
    23341 aaaggatggg atttctgtag cgggtatgtt gtcgggaaat tttgaacaag gcaataggat
    23401 ttatgaggta accggaacag cccccacttt gtcaacgaaa caagggggaa cgaagattat
    23461 gattcggaaa aatgaaacag aatatcaaga ggtgaagcca ggggacagtg tgaacctggc
    23521 ttttcctaat tccacaagtc gccgaggtcg tctgggaaaa caaagtgtgc ataccttact
    23581 aacaggagac caacaagcgg tagtcacgga tcagtaccag attcgaaagt taacacctag
    23641 ggagtgctgg cggcttcagg gctttccaga ttgggctttt gatcgagctt ctcaagtcaa
    23701 ttcagatagt cagttgtata aacaggccgg aaactcagta acagttccgg tcatttttga
    23761 tattgctaga agattaaagg gggtaaaagc atatgattta tgacgagttg attggagaaa
    23821 tctactggat gattggcaag attcaggcag atccgaaatt ggaagaagaa ctgtaccggc
    23881 tcaattttga tattcgcaaa aacggggtga aagtccaggt gattcgtatg tagtggatga
    23941 ggaaacggat gctcgaatgg agctcaatca agtcatcggt gaattggaac gaattgcgga
    24001 ccttgccaaa gaacctgata tccgccaata cttgtttgag ataaaagcag agttggaaat
    24061 tgagggtatc accgcggagt aatagggaaa taacccactg ataggcactg accgcggggg
    24121 atttgcgaag cagaaatcct ccgtggtgct gtgcctgttc atggacggcg gcagccggcc
    24181 atgatgtctc accccccgta tctaacaggg gggtacaaaa acacaaaaaa caagcaagta
    24241 accataaaaa atgcttgttg tatcaaggat attaagtaat ttgtataggt gttaactcat
    24301 ttggaaaagt taacaccttg aatttggagg tgaaaacttg gcgcaacgaa atttagatta
    24361 tcggttatta aaagaacgac gcaacgaata tggcgtttca caaaataaat tagctactgc
    24421 ttgcggactt agccgcccat acctaaatca gatagaaaac ggtggcgtaa tcgcctctac
    24481 aaagaccatg agaaaaattt ttgatcaact ggaaagtttc aatcccgatt tacctttaac
    24541 gttactattt gactatgtaa ggattcgctt tcccacaacg gatgtgcgga aaattattca
    24601 agaaattctc catttgaaat tcgagtatat gcttcatgaa gattacgcct tttactccta
    24661 tcaagaacaa tatgtcatgg gcgatattgt agtgatgtta tctcatgaag aagataaagg
    24721 tgtccttttg gaattaaaag gtcgtggttg ccggcagttt gaaacttttt tactcgccca
    24781 aaagcgtagc tggtacgact tttttgaaga ttgtctaaaa gccggtggcg tgatgaaacg
    24841 cttggattta gcaatcaatg atcgagtggg gctactggat attcctgatt taacgaaaaa
    24901 atgtcagaag gaagaatgta tctccttgtt tcgtaccttc aaaagttacc gttccgggga
    24961 actgttgaaa gctgatgaaa aggatggtat gggcaatacc ttatatattg gcagcctcaa
    25021 aagcgaggtt tatttttgtt tgtacgaaaa agattatgaa caatatatca agttagggat
    25081 tccattggac cagaccgaaa cgaaaaatcg ttttgagatt cggttgaaaa atgaccgtgc
    25141 ttattatgcg attcaagatt tgttaaaagg tcgtagcatt gaaagtacca cgttttccat
    25201 tatcaatcgg tatttgcgat ttgcggataa agtagatgga aaacggcgaa ccaattggcc
    25261 cttgaatgaa caatggggtc gttttattgg acggaatcgg aaagagattc aattaacttc
    25321 tgaaccgaaa ccgtatacca ttgaacgaac attaaattgg ctaggtagac aagtcgcccc
    25381 tacgtggaaa atggctaagg agttggatcg attgaatcaa acaacgtata tccaggatat
    25441 ggtacagaat gcgcgactct cggaccggca taaaaagatt ttggagcaac aaagtatggc
    25501 aatcgaaaac ttgattgtat gaaaagagga attttgatgg aaattgtaaa agatatgtta
    25561 ttggtacttg gtggcagtat gcttggtgtt accgtgatgt gcttgatgaa tgcaagtgca
    25621 gcagcggatt gagcaatgga aaagaaagga aagtgacaaa atgaatttcg gacaaaattt
//
LOCUS       DAZULG010000026        25482 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000026 DAZULG010000000
VERSION     DAZULG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.026"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(157..1110)
                     /locus_tag="ACG9CY_001015"
     CDS             complement(157..1110)
                     /locus_tag="ACG9CY_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4937541.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1133..1888)
                     /locus_tag="ACG9CY_001016"
     CDS             complement(1133..1888)
                     /locus_tag="ACG9CY_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937542.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(1885..2844)
                     /locus_tag="ACG9CY_001017"
     CDS             complement(1885..2844)
                     /locus_tag="ACG9CY_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4937543.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(2837..3787)
                     /locus_tag="ACG9CY_001018"
     CDS             complement(2837..3787)
                     /locus_tag="ACG9CY_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4937544.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            3937..4389
                     /locus_tag="ACG9CY_001019"
     CDS             3937..4389
                     /locus_tag="ACG9CY_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS4937545.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4478..6745)
                     /gene="gshAB"
                     /locus_tag="ACG9CY_001020"
     CDS             complement(4478..6745)
                     /gene="gshAB"
                     /locus_tag="ACG9CY_001020"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS4937546.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(6932..7930)
                     /gene="ftsY"
                     /locus_tag="ACG9CY_001021"
     CDS             complement(6932..7930)
                     /gene="ftsY"
                     /locus_tag="ACG9CY_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS4937547.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(7941..8750)
                     /locus_tag="ACG9CY_001022"
     CDS             complement(7941..8750)
                     /locus_tag="ACG9CY_001022"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4937548.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8759..12340)
                     /gene="smc"
                     /locus_tag="ACG9CY_001023"
     CDS             complement(8759..12340)
                     /gene="smc"
                     /locus_tag="ACG9CY_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS4937549.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12360..13046)
                     /gene="rnc"
                     /locus_tag="ACG9CY_001024"
     CDS             complement(12360..13046)
                     /gene="rnc"
                     /locus_tag="ACG9CY_001024"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS4937550.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13394..15175)
                     /locus_tag="ACG9CY_001025"
     CDS             complement(13394..15175)
                     /locus_tag="ACG9CY_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4937551.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15201..16115)
                     /locus_tag="ACG9CY_001026"
     CDS             complement(15201..16115)
                     /locus_tag="ACG9CY_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4937552.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16142..17104)
                     /gene="opp4B"
                     /locus_tag="ACG9CY_001027"
     CDS             complement(16142..17104)
                     /gene="opp4B"
                     /locus_tag="ACG9CY_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4937553.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17104..18048)
                     /locus_tag="ACG9CY_001028"
     CDS             complement(17104..18048)
                     /locus_tag="ACG9CY_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937554.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18049..19053)
                     /locus_tag="ACG9CY_001029"
     CDS             complement(18049..19053)
                     /locus_tag="ACG9CY_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937555.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(19321..19563)
                     /gene="acpP"
                     /locus_tag="ACG9CY_001030"
     CDS             complement(19321..19563)
                     /gene="acpP"
                     /locus_tag="ACG9CY_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4937556.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(19685..20686)
                     /gene="plsX"
                     /locus_tag="ACG9CY_001031"
     CDS             complement(19685..20686)
                     /gene="plsX"
                     /locus_tag="ACG9CY_001031"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS4937557.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(20735..22771)
                     /gene="recG"
                     /locus_tag="ACG9CY_001032"
     CDS             complement(20735..22771)
                     /gene="recG"
                     /locus_tag="ACG9CY_001032"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS4937558.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(22888..24570)
                     /locus_tag="ACG9CY_001033"
     CDS             complement(22888..24570)
                     /locus_tag="ACG9CY_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS4937559.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(24622..24984)
                     /locus_tag="ACG9CY_001034"
     CDS             complement(24622..24984)
                     /locus_tag="ACG9CY_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4937560.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            25254..25442
                     /gene="rpmB"
                     /locus_tag="ACG9CY_001035"
     CDS             25254..25442
                     /gene="rpmB"
                     /locus_tag="ACG9CY_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS4937561.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         6945 a         5593 c         4492 g         8452 t
ORIGIN      
        1 aagatttctc aaataaataa aaaccatccg taaaatcatc aatacgattt tacggatggt
       61 ttttgataac ttctttgagg ataggctctt gctatttaag aatgtaagaa acattattgt
      121 tacatagtat gagagtttaa tcctctgtta tttatgctat ttaaatgctt gattcacatc
      181 ttcgatcatc agtttcattg attctaaacc gcccccgctt aaataccaga catctggttc
      241 aagggagatg atctgctgat ttttaccagc attcgtttgt gctaccagtt cattggcaga
      301 aatatcatct ttgctgtcgt ctccaccaat agcttttgtt ctgtctacta caaataagat
      361 atccggattt ttttccagca catattcata agaaacactt tgaccgtgag tggatgcttc
      421 gatttgatca tctgcttgtt caaagccaaa taaatcatgg atgaaaccga atctagaacc
      481 agatccgtag gctgataatt gcccttcatt cacaagtgtg actagtgctt ttttgtcagt
      541 agcttctgct ttctttttcg tctgatcgat tgcactagat aattcctcta attgagtttt
      601 agcttcttct tgcttaccaa agatctcccc taaagcagtg acattttgct ggaaagattc
      661 ccatggattt ttcgcatcta aagaaaggaa aatggtcgga gcaatttgac ttagatcttt
      721 ttgaaaatct ctttgtcggc cagaaatgat gattagatct ggctgaagct gattaatttt
      781 ttctaagtct ggttctttta ttccacctgc tgattctact tttttatagg atgaaagata
      841 ctcaggaagg ctgcttgtag ctgctccaac taccttatct ccaacaccaa gagcatccat
      901 cgtatctaaa gaaccattgt cgaatacgac gactttttct ggattatttg gcacctcgat
      961 ttggtctcca ttactatctg ttatcgtcaa tgtagtttca gtttgtgtgc tgcttttttt
     1021 tgtgtctgcc tgactggtgt tgttgctatt tccacatgct gctaaaccaa atgatgctag
     1081 aatcaagata cctactacta attttttcat ctcttctctc ctaatgttag ttttagaaat
     1141 aaagacagaa tctgcggcct tcaatctcac aaatccgtat gtccatttcg tataattcat
     1201 ccaatacatc tttttttatc atttcttggg tcgttccttg cgtaaataac tgtccatctt
     1261 tcattgcaac aatttcgtct gcataacttg cagcaaagtt gatatcatga agaacaataa
     1321 caattgtttt cccgatttct tgtactaaat tttttaaaat ctgcatgatt tgaaccgcaa
     1381 aattcatatc tagattgttc aaaggttcat ccagtaaaat ataatccgtg tcttgcgcta
     1441 gcaccattgc aatatacgca cgttggagtt gaccgcctga aagagtgtca atcgattgat
     1501 ccgctatttc ttctaaccca aggtgttcca gtgattcctg aacgatttcc tcgtcttttg
     1561 tcgtcaattt tcctttcgta taaggaaatc gtccaaattc tactaattct ttcacggtga
     1621 tttggacatt tatttcattc gattgtcgta ggatcgataa ttttttggct aattcattgg
     1681 atttccaagc ctttacttcg ttatggtcta aatagatttc acctgtatct tttggtatca
     1741 aacgactgat catcgacaac accgttgact ttccagcacc atttggaccg ataaaagcag
     1801 tcaacttctt ttcaggaatc gttaactgta catctttaac cactgatttt ccatggtaca
     1861 gtttattgac cttttgtaaa tcaatcatag gatactcttc ctttcgcgaa tcaattttgt
     1921 gacaaaataa attcctccga caaattcaac gacgacactc actgttgtat tccagccgaa
     1981 aatctgctct actgcgagct gtcctcctgt cagaaacagc atagctacca gaaagcttcc
     2041 gataaatagc tgaccatgac gataagtaga aaagacacga taactgatat ttgcgacaat
     2101 aaaccctaaa aaagtaactg gacccacaag agccgttgat gtccctgtca aaagactgac
     2161 acaacataaa atcaaaaatt caaaacgatc tgtgtctatc ccgaggttta ctgcttgttc
     2221 atttccgaga tggaaaacgt ctagcacatg tcgatatcga aataacaggt accagcagcc
     2281 gataatgatc aaagaagcag gcaaaagata ctgactctgg atattgccaa agcttgcaaa
     2341 taatctgcct tgaagcaaat catattcatt cggatccatg atgacttgta aaaaggtact
     2401 gacactacca aaaagcgtac ctaaaatcat ccctgacatc agtaataaaa aaagattcgc
     2461 tctcattttt ttcaaaagaa agaaaaataa caatagactc agcccgacca tcagcccaac
     2521 gttcaataag aaaaaaactg tacctgtctg ctgaagcatc gtcactcctc cgataaagaa
     2581 gaaaaggacc gtttggacaa ggacatataa cgactcaagt cccaatactg aaggagtcaa
     2641 aaactggtta tgcgtaattg tctgaaagct gattgtggca ctgctgacag ctagcgagac
     2701 aaggataaat gcaaaaattt tcttactcct caatgcacat gcaaaatgcc agtttccata
     2761 tgtatggatc gtcaagtaca ttcctgtaac aaataaaaca agaacaagca taccaataaa
     2821 aatagatggc ttttttttaa gcatatcgtc gtccccttat cagaagataa aggaagatag
     2881 ccccaccgat gattccaacg atcacactga ctggtatttc ataaggaaaa atcaacacgc
     2941 gagcgacaag atcgcaaaat actaagaaca aactacctgc taaagcagtt acgccaattg
     3001 ttttgtgcac ttgatctcca tatattctag agacaagatt cggaatgatc actccgataa
     3061 aaggaatgct tcctaccatg atcaacatga ctgcactagc caatgatact aagccaatgc
     3121 ctaaaaactg catcttctgg taatctattc ccagatttac tgcaaacgat tcgcccagac
     3181 caatgatcgt aaatcggtaa gcaaatagcc aaaccaagag caacaatgga acaacagcat
     3241 acaataactc ataatttcct cgtatcacag tcgcaaaatt cccttgaagc catgaagtca
     3301 tattttgtac caactggaat tgataagcaa aaaaagtagc aactgaacca atgatattcc
     3361 ctaacatgac tccaatcaac ggaagcatca attgattttt ctggaggaac aatcgcgtaa
     3421 gggataaaaa aagcaatgtt cctgcaaaag caaagcaaaa tgctgtcaag gatcggacaa
     3481 tcaaggatgc accaggtagg aaaatcatag ccactaaaat acctaaacgc gcactatcca
     3541 tcgtgcccgc agtcgttggt gaaacaaatt tattttgggt caaatgttgc atgatcaacc
     3601 cacaaatact actggtcgct cccacaagga tcaaacaaat cgttcgtggc aaacgagtca
     3661 ctagcaagat ttgtaagctt tgcccatcta actggagcag ttctgacaat tgtacattcg
     3721 atactccgat gaacaatgaa atactactta aaataactaa taatgctact agtcgtccgt
     3781 agcgcatcca atccaccttt ctaattgaga atgattttca ttcttatttt aagtgaatcg
     3841 ttgaacaaaa gcaaccgctt tttcatatgt tttaggtatt attgggaata tgctataatc
     3901 gtaagaaaga caaagagaag aaggagggta ttttttatgg cacaagatga tgtaaaaagc
     3961 catttggata agggacttta tggtacacct ttagtgaacc ctgaagaaca gcataaatat
     4021 ttagggacat tcagggaacg ctgctatctt agtatgactg ttgctgaaat gatccaacca
     4081 aaaaacaaag aaaacttttt aaaagaactc aaaaaacatc cagatgcttc catactcttg
     4141 aatggggctc tacccctttc tgtccaaaac caatatatcc agctagcaac gaaaaacaat
     4201 agccgtttca ctgtagtcaa cgattacgtc actgcaaatc ctgaggaatt cggattgctc
     4261 ttaacagcaa aagaagcagt aaatgaacct gtgattgata tcgaaaaaaa atacccagaa
     4321 gaaaactctt cgccagctga aaaagaaaaa ccaaaaaaga aaaaaagctt ctgggatcgt
     4381 ttattttaaa acgaaaaaac acataaattt ttacatgcaa aaaaagcttt tgatcagatt
     4441 gacatcaaat aggacaatct gtcaaaagct ttttctttta aaatacttca ggatataaca
     4501 atttcaatac attcatcgtc aatcttctgc cttttcctgc aaaaggataa acatgcatat
     4561 gcatggctgg attgaaattt gcttcgatga tcccatatgc ttttttatca gttgtaggat
     4621 cgatttcttt atctggtata atcaaatcga taccactaat cttagctcct agtgcttcta
     4681 ccgcagaaac ggcgattttt ttgtaagctt cagaaaattc atcagtcata tcgatcgaat
     4741 caccacctgt actgatattt gagttctccc ttagataaac aatttgattg gcttggggaa
     4801 ctgattcgat cgtcaaccct tgttctttca acatcaattg ttctaattca cctaattgga
     4861 tcaattctaa aggtgcacga tgattcgttc cacgcaaagg gtcactattt ttttcttcaa
     4921 caagttcttt cactgttcgg atactgtcac caataacatt agcagggact cgcaacatga
     4981 tggcttggac ttctccgtcg atcacaaaga aacgatattc cgttcccggc ataaattctt
     5041 ctactaaaac cgaactgtct tcacgaaaag caatggctaa acctgcttga taatcttcca
     5101 gactagctcc ttccttgaaa atcgtgatac ctaacccgta attggttgat ttaggtttga
     5161 tcacaaatgc ctgctcggca aatctcggat aagcttttac cgcttcttcc atggatgaaa
     5221 attctgcgcc tcctggtacc cggaacccag cttcttttaa gacttttttc gttactgttt
     5281 tgttttccat gataagtggg acgatatagc tgtccttact tgtcatgttt gcatttttca
     5341 catattctac atggtcttgg aattgcagac gcaaaaattg atccgactca tctaatactt
     5401 tgacttggac acctttttgg atggcatcga acataaatat ctgagtagaa agttccattt
     5461 cgcgaaaacc ggccaactga tagggacgtt catggctttc tttataatat tgatgtccca
     5521 atgccacagc aacttcttgc tgactcgata gctgtgcttc ttgatagagt ttcgctgcta
     5581 acgtttgttc aggatgatcc atttgagtca atgcttcctc tactaaataa agagaatcca
     5641 accaatccaa ttcaaccagc atttgcttca ttccttcaag aatttctcta ccttcttgat
     5701 gaaattctgt ttgatctgat ggttgttcta gtgctacttt gttatttcga gtcgtacctt
     5761 ctgctaccca ttggtcacag tcttcttttt cttctgtcca taacatgtac atcaggaata
     5821 attgtaagaa ccgaacttgc tctggattga tacccaatct tgcataagga ttcaaatcga
     5881 tattccgcaa ttcaatataa cgaacacctg ttgttgctaa atctgcgact ttctttccgc
     5941 ctcggaaacg aagcggtgta taaaattctt tctcctccga aagtttccct tcttcgatca
     6001 ttcgctcgat atctgctaaa tactgtttca tagaagcata ggaaactttc acatttggat
     6061 gattcgtata gcccagcgca ctgttgcgaa tactgcgaac aggttcttgc ggatgtgatt
     6121 catctaaaaa gtagttcttc tcactcattg gtgaagcacc gaataaataa gtgatcatcc
     6181 agcgatagcg cataaagttt ctcgctgttt tcaaataaag ttctgttttg aattcggaaa
     6241 aatcttgaaa ttcttcctga tggctgaaca atgttcttag caactcatcg ccaaattcaa
     6301 aattgaaatg aataccgctt accatctgtt ttcgcttccc gtattttttt gctaaataac
     6361 gtcgatacaa gacatcttcg aaatttttta attttgcaat aatgatctct tcgtcttttt
     6421 ccggtaaggc aggtggcata cttaatggcc agatcatctc ttctttcggt atagaacgag
     6481 cagtcacatc ataaacagca gcgagatact gaaataattc gggaatagaa tcggtaacag
     6541 gggtgatcat ctctgtttgt gtttcgctaa aatctgtttg gatatagggg tgataggatc
     6601 gatcgccaaa gattgctgga tgatcggttt ttgctagatt tcctgcaaga tcaacccgct
     6661 gtccttcccg ttctatacca aatcgagctt ggtcgataaa agggcgcgca ttgacatgca
     6721 ataataattg cttaaaattc atcattaagt gacctccaat tcatatgaat ctaattataa
     6781 tcaaaaagtc taagaaaaag aacgaaattg tttgagaacc gcttacaaat aagtataaaa
     6841 atgaaggcat caaaaacaca gctatgttaa aaacaatcat tcaaacaagg agctgcaact
     6901 tttgtcacag ctcctgcttt ttagacatgg gttattcttc ttttagtaat cctttgaaca
     6961 atccaacgac aaaatcattt ggatcaaacg gctccaagtc atcgattcct tcaccaagac
     7021 cgaccaattt cactggtaaa tgcaattcat tacggatagc aagtacgata ccgccctttg
     7081 ctgtaccatc taattttgtc aaaacaagac cggtcacatc tgttgtttct ttgaattgtt
     7141 ttgcttgaac catcgcgttt tgaccagtgg ttgcatctaa tacaagaagt gtttcttggg
     7201 gtgcttctgg taattcccgc tggattactc gttttatttt ttctaattcg ttcatcaagt
     7261 tcactttatt ttgcagtcgg cctgcagtat caactagtaa aacatctgcc tgctctgtct
     7321 ttgctctttc caaagcatcg aatacgacag ctgctggatc gcctcctgca ttgccacgaa
     7381 caacttcaac accagcgcgt tcgccccaga caactaactg gtcgattgct ccagcgcgga
     7441 aggtatctgc tgctgccatc aatacttttt taccttcgtt tttgaactga tgggcaagtt
     7501 taccaatact tgttgttttc cctacaccat taacaccgac aaacaaaata actgttagtc
     7561 cgtcttcttg gatatttagt tcatttactt cattgattcc ttccgcttca tagagatcca
     7621 caagtttctc gatgatcgca ttttggactt ctgccgattt tttcgcattt ctgagtttca
     7681 cttcttgacg taatgcttcg gtgattcgta tcgctgtttc aaaacctaca tctgcaccga
     7741 taagtgtttc ttctaattct tcaaaaaagt cttcatcgac tgttcgaaaa ttggcaaaca
     7801 actcattcat ccgttcacca aatgttttac gcgtcttttc taaacccttg tcgtacttct
     7861 cctgtacttc aacttcttct gttttctctt cgccagtaaa agcacgcttg attttatcaa
     7921 aaaatcccat ttgactcccc ctactctaat acaaattttt ggatcacttt ggctacgcca
     7981 ttttcttcat tgctggctgt cacgaaatct gctgcttctt tgacgaaagg cacagcattc
     8041 tccatcgcta cgcctagtcc agcatattgg atcatcggta aatcattttc ctcatcaccg
     8101 atcgccatga tttcttcttg tttcaaacca agatcttttg ccaataaaga aataccataa
     8161 gcttttgtaa caccttttgg cataaattcc aaaagcatac ttcttgtttt gatgatctca
     8221 tacatttcat ggaattctgc tggtatttct ttgattttag gatctaattc ttcttggtca
     8281 taagcgatta ctgccttatt aagcacaaat tcatctgtaa tatcgcttat atccgctggt
     8341 tggaattgca gcagcttatt caacacattg tacaaggatt gcttccttgg ggcactaggc
     8401 agctgtaaaa ccacattgtc agacaaaaca tctagtggca gatccaattg ctgagccagt
     8461 ttgtataagc gttggatatc tgatacttcc atcactttct tttccatgat ttcaccagta
     8521 tcattttttt gtaccaaacc gccgttgaaa gtgatgctgt agtcaccttc atctaccagt
     8581 cctaattctt gcagataatg cgccatcgca gctaaggggc gtcctgtaca tagaacgatc
     8641 ttgacccctt gctgttttgc ttgtgctaat gcttgtttat tctcatctga aatcagcttt
     8701 tgttcattta ataatgtacc gtccaaatcg attgctacta atttgatcat tttcgttcct
     8761 acactttcgt ttcgttgatt ttcgcctctt cattgatatc ttccatccgt acagaaacga
     8821 tttttgatac accggattcc tgcatcgtca ctccgtacag tacatctgct gcgaccatcg
     8881 ttcctttgcg gtgtgtgact acgataaatt gtgtgtcatc ttggaaatcg ctcaagtaac
     8941 gaccaaaacg tttcacgttg gcttcatcta acgctgcttc tacctcatct aagatacaaa
     9001 acggaacagg acagacacgg ataatggaaa agagcaacgc gatggctgta agtgctcgtt
     9061 ctcctccaga aagcaaactt agactttgca gttttttgcc aggaggttgt acttcaatct
     9121 caatacctgt tttcagcaaa tcagttggtt ccgtcagtac taattcagct ttaccgccgc
     9181 caaacatatt tggaaagaca attttgaatt cttgacggat agcttcaaat acttctttga
     9241 atcttgtccg cacttcatca tccatctcat ccatcgtttc aaacaattgg tttttcgctg
     9301 ctaacagatc atctcgctgt gttgctaaga atgtatgtct ttcactgact tgttcgtatt
     9361 gttcgattga atttaagttt actggtccca gtttttcaat ctgttctttt aaggatgaaa
     9421 cctttgttct actgtcttcg atatcggttg tttcttgata gtcttgggaa gctttctcaa
     9481 atgtcagctg atattctgac tgcagatagg tcaaatgatt atctaaaatc atttcagctc
     9541 ggtctttttg gacttctaat ttggattgtt ccgacagtcg tgctttttgc tcacgattac
     9601 gctctgccaa gactgcttca agcttatcga tctccttttg ccttctatct cgctgctctt
     9661 tggcttttgc caactcggct tttaatgttt ctcgctgagc agataattcg ttaatttgtt
     9721 tttccaaact ttcttctgtg acttcatgat cagaaaagtc tgctgtcaat gtcgccaact
     9781 gcttttctat cgcttcttga cgttcggttg cctctgcttt ttggactcgg gcaccgcgga
     9841 gctggatctg cagatgatta aactgctctt tcaagacagc aagatctgct tgttcttgtg
     9901 ctttttgact ttgtacttgt gctcttctgg cttccatctg gtcgctttct tgacttaacg
     9961 atttgatttc ttcgtcgatt ttttgtcgtt gcgattcaag atctgtttgt ttgtctttca
    10021 attcgttcgt ttgcttttcg taatcttcga taaactgctg cagttcacgt gtctcaaaag
    10081 ttgagatttg cttttctttt tcaaaacgtt caagttcatt tatgatgttt tgtagctgat
    10141 ttgtcgcttc ttgttcttca aaacgtaatt gttcgcctcg tgtacgcaaa acttcttgtt
    10201 cttcggctaa acgagctgtt tcttgttgca gctcctgaac ttttttttct tgtgcttgga
    10261 gttgtttatc tgcttcttca aattcactag tcaactgttt caattcttga ttttgtacaa
    10321 acaaactgcc ttggtttcct cgtttattcg ctccacctgt catggatcct ccggcattca
    10381 tcacatcacc ttctaaggaa accactcgat attgatagcg gatcgtttgc gcgattgcat
    10441 tggcactagt taagtctttg gctaaaagga tcgttcctag caggttttga accacagttt
    10501 ggatttgatc gggataactg acttgttcac tggcaatccc tataaaccct tctacagctg
    10561 ctgcttgtgt caatatatga gccggtaatt gtcttggttt gattgtcgtc agtggaagga
    10621 atgttgctct tcctcctctt tgctgtttca aatacgtgat ggcttgccga gcatcttttt
    10681 cattttcaac gatgacatgc tgggctgccc cacctaaagc tgcttcgata gctaacgtga
    10741 aatctgcagg aacatcgatc agttcagcca ctgctccgac aatacccgaa agctgctgtt
    10801 tatgctgcaa gaccaatcga accccttggt aaaaaccaaa atagttttct tggatttctt
    10861 gaaggctttt cttacgagca cgtagctgct ggacttgatt cattaattga tacattttag
    10921 gctgttcttt tgctaatgct tcttggaact ttttcccgtt ttgttggatc atttctaact
    10981 tctcttttgt ttcagttaaa gatgatttta gctgcgcctg cttttgtgtc aattcttctt
    11041 tttgtaagat caatcggtct actgatgctt ctacttccga ttgcttggct aacgtctgct
    11101 tgctttttgc tgtttcttgt atatattgac gttctagata cttcagttcg tttcctacag
    11161 ctgcttgttc ttgcatcaaa tcaacatatt gatctcttaa ttctgccaat aattctttcg
    11221 ttgatttttg gtatttttct aattcttgct gtgtttttat gagatttgct tcagcttttt
    11281 ggatctctgt ttctttttct gctgctgctt tcatcaagct ttcttgaagc ttttcaaaat
    11341 gtttaacctt tttttgtgct tctgctaatg aagtttgata ttcttgcgag cttttttgtg
    11401 tatgctttgt ccgctcctgc aaaacatctt tttgtccttc tgtctgcttc agtttttccg
    11461 aaagatctaa taatacttgc tgattttttt cgatcagacg atcggcttgc gcattttctt
    11521 tacgctgctt cgccaaaatg gattcttgtt cttggatcga ctcagatagt ttgcctaatt
    11581 ctagattgaa tttcgcaagc tgtgcttgtt tgttgtccca atcttttttt gctgtcttga
    11641 tttcagcaac catcaaagag acatcggttt gagtcagtgt ttcttttagc cgtaaaaact
    11701 cctttgctgc ctcactctgg gcggccaaag gagttaactg ttcttccaat tcgtggatga
    11761 tgtcttggac gcggctgagg ttgtcttctg tttcaaaaag cttctgttct gctttctttt
    11821 tccgctgctt gtatttcaag acaccagctg cttcttcaaa gatcccacga cggtcttccg
    11881 gtttactact gaaaatcgct tctacttttc cttgagaaat gattgagaac gattcttttc
    11941 ctaatccaga atctaagaac aattcttgga tatcttttaa tctgcagctt tgtttattga
    12001 tgaaaaactc gctttcccca gtccgtcgat aacggcgggt tacactgatc tcattgtact
    12061 ccaatggcag ataatgatca ctattgtcta aaatcactgt gacttctgca atattcagct
    12121 gcttacgggt atcggaaccg gcaaagatga tatccggcat ttttccgcct cgcagacttt
    12181 tagcagattg ttctcctaat acccagcgga tagcctctgt aatattgctt tttccgctgc
    12241 catttggtcc aaccactgct gtgacgctat tttcaaaatc aatgactgtc ttgtctgcaa
    12301 atgatttaaa accggctatt tcaattcgtt tcaaatacaa agctttccct cacttacttt
    12361 tacagtgctg ccaatgcatt ttccgccgca gcttgttctg ctaatttttt cgatttccct
    12421 tgtccagtgc caatcaattg atcatctaca taaacttcga tccaaaatac tcgttcatga
    12481 gctggacctt cttcattgat caatcggtat tcgattgata catcacctga tttttgcaag
    12541 acttcttgta gttttgtttt atgatccatc tcatgtgaaa aagcacctgc tttgacttta
    12601 ggaaaaatca ctttttcgat gaatgcatgg gtcgtgtcga atccctggtc aagatataac
    12661 gcgcctaaaa aagcttcgaa taggtcgcaa agcaatgctg gtcgcgtacg tcctcctgaa
    12721 ttctcttctc ccttgcccaa caaaacatat tgatcaaagt gacattcttt ggcaaatttc
    12781 gacaagctgt cttcgcgaac gatcgcagct cgtgtttttg tcaattttcc ttctggcaaa
    12841 tgaggatata aacggaataa atactgagaa accatcagtt ctaaaacggc atctcctaaa
    12901 aattcaagtc gctcattatc cgataattgc agattgcgat gctcattcac ataggatgaa
    12961 tgggtaaaag cttgttcaag caaatttaga tcgtgaaaaa cgatgttata gttttctttt
    13021 agttcttttg tcagctggtt gtccattttc cacattcctt ttatggatat tttttcgata
    13081 cttccttatt atactggttt tttgtcaaat ttgaaatggt tcggtcaaaa ggtaagtaaa
    13141 gctttaataa tcctagtgat tgacgagaag ttgtgaaaaa ttcgtttagt ttcgagtatg
    13201 aagaaaaaat tttgtgaaaa tggctttcca tatttttgca taaatttgac ttaataccga
    13261 gaaactggac tttgaacacc gtttattgga gaagtcatga aatcagcgcc ttgacctgaa
    13321 tattagagaa ataaacagaa aaatatcaaa aaaccgaaaa aggacatcgc cctttttcgg
    13381 tcatttttag tgattatgaa cgtgaatctg ctgtaacaga aacatttgcc catgggtttg
    13441 gtacatcata agaccaatcc cagtctttga cacggtcgtt gactggcaat acttcattac
    13501 ggaacaacgt tggaatcgca aatgcttctt caaatgcata ttgttgccat ttttgatatg
    13561 cttctttttg gtactcttca tcaaatgctt tgcttgaact gatatcgttg atcaattttg
    13621 tattttcttc tgattcataa cgagtgtagt tgaacgcagc atttggtccc catagtccac
    13681 ttggatttgg atcagttcct gtattccaag cagcttggta aacatcaatt tcaggatcat
    13741 cgttttctaa tttatcatag aaagaattga attcgatcag acgacctgtt gtgtattgga
    13801 catccaaacc aatttctttc cattgttgga tatagtaatc tgccaatggt tgcgctgttt
    13861 ccccaccgga catcgaagca aagttgattg ttagtttatt tccatctttg tcttctctta
    13921 aaccatcgtc atccacatct ttgtagccag catcatctaa tagttttttc gctttgtcta
    13981 gatcttgtgt gaatcctttc atctcatcat catggtatga ctggaatact ggaggaatca
    14041 atgaagtcgc attgcttctc aaacctgcat agaaacgttc acctactgca ttattatcta
    14101 atgcatagcc cattgcttga cgtaatgatt tatcagccat ttttgaatct ggattgtatt
    14161 ccacactctt tgtatctgca ttccatttac ctaacttgaa tcctagataa gtataagaag
    14221 tttgttcacg gcctaagttt gtatagccag caatttcttt ataggtatcg taagtatctg
    14281 ttggcatttg gaaaatcata tcatataatt tagagttcaa tgcttcagtt gcacttgcca
    14341 tcggtacaga tttcatcgtg atctttttca atttaggcgt accattccag tagtgctcgt
    14401 ttggcaagta ttctactgat tctcctgcaa cgatattact catgtaataa gggccaaaag
    14461 tcactggatt tttacgtact tgatcgcttg attccatatc tttgattgct acaccttcta
    14521 acgtatgttt tggtgctgca tatgaccaga taccaccacc cgattgaagc atgcttgggt
    14581 ttacttcttt atattcgatt tcaactgttt tgtcgtctat ttttttgatt ccggagattg
    14641 tgtcggcact tccatcatga tattcttcca tacctacgat gttacggaaa gtgtcgtcat
    14701 aacggatacc agtgtaatct ttattcccaa tgatttcgta agggaaaatc acatcgtccg
    14761 ccgttacatc ttcaccatct gaccatttga cgttgtcttt tactttgatt gtcgcttttt
    14821 tcgtatcttc atctagatca agcgttgcag ctccgtcgtc tgtgattctg aaatcgtcat
    14881 cgtttgcaaa tagcggttca tgagaaggag ccataaaagc tgcatcatat gaatcttggt
    14941 aaaactccca ctggaacaat cctttaaact gactgtccgt tgcgaccgca gcttctaatt
    15001 cgccgtcttc gattgcatca tcattatttt ttgttgcaat tggaaaagtt aattcctctt
    15061 tgttattgcc gttgcctcca gagttgtttc cagaggtatc tgtacctccg cccccgcatg
    15121 ctgcaagaac gacagcagac atcagcgctg caaatccaaa aatcgttttc ttcattccta
    15181 tttccccctt cgcttctata ctagcctaac cgttggcgcg catctgcaga acgcttgaat
    15241 gcttgtccaa cataattgat actcaacatc aaaactaaaa taaggattga tgcgggtaac
    15301 cagatccatt gccgattcac taagacttct ccgttacttg cataaccgat caatgtacct
    15361 aaactaggta catttgccgg caagccgaaa cctaagaaag tcaaagttgt ctcgataccg
    15421 atgtttgccg caaagttcat cgtcaaatta gtaatgatta atgaacttag attcggcatg
    15481 atttcgcgga acatgatttt aaaatcattg gttcccattg tttttgaggc actgacataa
    15541 tctcttcttg cttcagataa tgtcttgctt cggaataatc gggctttagc tacccaataa
    15601 aatgcactca tgatgaatat aaaggaccag acattgtatc tggaaataat cgatacgaag
    15661 acgataatga tcagcatgat cggtagaatc atgataaaat caacgatccg catcaagatg
    15721 ttgtcaatga tccctccgta gtagccagag atgatcccta atccaacccc gatgatcgaa
    15781 gtaaggatcg tgatcgcaaa tccgatcgta acagagtttc tagcacctat gatcaattgt
    15841 cctaatacat cacgtccgcc ttcatctgct cccagaatga acttttgtcc ttgtgtcgaa
    15901 actgttcctg gtgcagcgta tttgtcgaag atactaacat acatcacttt gtcttgatcg
    15961 gtcagcatcg caccgataaa gatcactaat aataaaacca ctagcaaacc taatgaaaaa
    16021 atagcaattt tatctttttt aaattctcga gcaatcattc gtatccccat aggaggaata
    16081 ctttcttgaa ctgctgtatt gtttttttca ctcatcgatc cattctcctt tctgattggt
    16141 tttattgaat ccgaatacgc gggtcaacga tactcatgat gatatctgaa agtaatgtcc
    16201 ccactagcgt cgcgattcct aaaatcaata ctaaggatgt gatcacggta tagtcccgtt
    16261 ggccgatgga atcaataaat agttttccga ctcccggata accaaaaatc ttttcgataa
    16321 ctacagaacc agcaatcaac gaagtgattt cataccccat ttgagaagcc atcggcaacg
    16381 ctgcatttcg gaaaatatgt cgactgaata ctttattcgt cggtacccct ttggaacggg
    16441 ctgttctgac aaaatcgagc gatttcgaat cgattacttc acttcgcaga tattggattg
    16501 tgacggcagt cgccaaaaat gcttgggtaa ttgatggtaa cagaatatat ttcaaacggt
    16561 ctatccattg agtgataaat ccaccttgca aacctagtgt ttgtgatcca cttgttggga
    16621 accagcctaa acgataacca aaaacaaata gcatgatcaa gccgaagata aataaaggaa
    16681 cagcaaaact aaagaagttg tagattacga caatcttatc caagattgaa ttttgatacc
    16741 ggccagcaag catccccaga ggcacagcaa tcaaataagt aaggacaact gtcaagatcg
    16801 atagatataa agtgttgatg atccgttcgc cgatcaagtc agcaacagag cgtttgtata
    16861 agaaactttg accaaaatcg ccttgcaaag catttccgat ccaacggaag tactggacat
    16921 accatgggtc attcaagcca gctgcttgtc gcatctgttc gatcactgcc ggatcctgat
    16981 ttggattgat caaaccggta aatggatccc ccggcatcgc ttgggctaat aagaagatca
    17041 gaacacttaa aataataact tgcgggatca tcaataaaac acgtcgcaga atcgttttcc
    17101 acattagctt gcacctccat ctttcaacgc cactttgtgt gtagttgtga tcgtacgcaa
    17161 atcgtagact cgaccagtcg catcatagta ttctttttgg ttttggatat actcttgttc
    17221 tactcttcgt cgattttctt tgtggctttc cctattttct acatcgattt gcggaatagc
    17281 tgaaagcaga cgtttcgtat agatatgtcg cgggtcatta taaatgtctt ctcttgtacc
    17341 gatttctaca aatcgacctt tgtacataat tgcgatattg tcgcacatgt gttttactac
    17401 acctaaatca tgtgaaatga ataaatagct gaggccgtat tcttgctgga ttcgtttcat
    17461 gaaattcaat acttgcgctt gaacagatag atccagagca gatacaggtt catcggctac
    17521 gatcagtttt ggattggtag caacagcacg ggctactcct agacgttgac gttgtccgcc
    17581 tgaaaattca tgaggatatt tatatagcgc atcttccggc atcccaacga tatctagcag
    17641 tccttttacc tttttctttt cttcttgatc gcttaaccgt tcaaaatttc ggatgggttc
    17701 agcaatgata tccaacactc gctttttagg attcaaactg gacattgaat cttggaagat
    17761 catctgcaca tccttgttat aacccatttt tttgcggttg cttcgcttcg ttacatcttt
    17821 tccttcatac atgatttgtc cggctgttac cttttctaat cccacaacgg cttttcccgt
    17881 agtagatttt ccagagccag actctcccac taaaccatat gtctttcctt tttcgataat
    17941 aaaatccaca ccatctacag catatacatg atccgttact cgattgaaga atccactgcg
    18001 aatagggtaa tgaactttca ggtctttgat tgtgattaat tctgtcatct acacctctcc
    18061 tttttcgtct ctaaagtgga aatgcttata gcaggagcaa cgtaccaagt ggttcggtgc
    18121 gacttcatga aggataggct cttcttcatg atcgcttgct ggtatccatg gaatacgtgg
    18181 tgcaaaccga caacctttac gaggcatgtt tttcagagaa ggaacgacac cttcgatcac
    18241 atggagttct gcatcatgat ttccttcttg tggtatagag ttcagtaaag agcgtgtata
    18301 aggatgctta ggatgatcaa acagttcttt tacacctgct acttcaacga actgtccgcc
    18361 atacatcact gccactcgat cagccatttc tgctacgact cctaagtcat gagtgatcaa
    18421 gatgatccct gtttttgttt cttcctgaag gtctttcagt aaatcaagaa tctgtgcttg
    18481 gatggttaca tctagggctg tcgtcggttc atctgcaatg acgattggag gtttgcaggc
    18541 gattgcgatt gcgatgatga cacgttgtct cataccgcct gatagttcat ggggatattg
    18601 gcgtccaact ctcgcaggat taggaatccc tacttgttct agcaattcta gtgcacgagc
    18661 ttgacgctgt tctgcattca tctttgtgtg ataggaaagt ccttctttga tctggtcttc
    18721 gattctcatg agaggattta atgcagacaa cggatcttgg aaaatcattc cgatatcatt
    18781 tccgcggatt ttattataga gcgtttcgtt caggtcgatt aaattcagat cgttatataa
    18841 aatctcacct gtaatttttg tgttattagg atcaagtaat cccataatcg tagtagccaa
    18901 ggtacttttc ccgcacccag attccccaac gattgctaaa atctcgtttt tgtctaaagt
    18961 aaatgaaacg tcatctactg catcgtaata gtcatctttg agtcggaatc ctgtatgtag
    19021 gttctgaact tctaataatt ggttatcgga caatcgaata cccctctctg cttcctaaat
    19081 ggaagttttt cttaataaaa aaggacatta cacagaataa agcgttttat aacaaaggtt
    19141 attcttatct ttgtgtaatg aattattaat aattatatag aaaatcgctt tactttgcaa
    19201 cataatttct gtttatcgcc agaattatca gaaaaatgac ttttacgtgt caaaaagatt
    19261 aaaaaaagaa ggataaaaag ctgtatgcag ctttttatcc ttctttgctt ttatttgaaa
    19321 ttatttaaga tggctgctga tataatctac tgcgccaccg actgtttcaa tttgttcagc
    19381 gtcttcatct gaaatttccg taccgaattc atcttcaagc tctaaaacga attccataat
    19441 actaattgaa tcagctttta aatcatcttt gatgttcaat tcatccgtca ctttatcggt
    19501 atctatctca aaatgatttg aaataatttt cgctactttg ttaaatactt cttcgcgagt
    19561 caactttttc acctccaaga atcatacact tgtcaattac ctacaccatg gctaatttta
    19621 ctggcatttt tcaggaatgt ccaccttttt ttcaatggaa cggattgctt cagatatcca
    19681 aatactattc ttcgcttttt tcaaattgtt cgactaattg tccaactaca tctgtctcaa
    19741 gcattgtgtg gatttggcgg atcgtatagc gaacagcatc ggggcctgtt gctccatgag
    19801 ttttaatgac tggtgctttc aagccaaaca aaacggcacc gccatgtttg gaataatcca
    19861 tttctgcttt caaagaacgt aagccgtctt ttaaaagcat agcgcccatt tttcctttga
    19921 tcccttcatt tagaatagca gattttaata gattcatcat attcattgct gttccttcaa
    19981 tagatttcaa aacagcgttg cctgtaaatc catctgtgac cactacatca gccacaccat
    20041 tgagtagctc acgagcttct acgtttccaa taaaattcaa gcttgtttca ttctgaagga
    20101 gttcataggc ttttttcgtc acttcactac cttttgttgc ttctgttccg ttgtttaaga
    20161 gtgcgacacg aggacgtccg atacctcgga ctttacgtgc ataaaaagaa cctaaaatac
    20221 cgtattgcaa caaatgttct ggtttgtttt ctgcgttagc gccaagatcc agcatatcga
    20281 aaccggcacc ttctttccct actactggca aggtagacat caagccaggg cgttcgatat
    20341 ttttgatacg tcctacgatg aacaaccctg ctgctagtag cgcgcctgta ttccctgctg
    20401 aaaatacagc atctgcttca ccgtttttca ctgcttgtgc agcaaggacc attgatgcag
    20461 tcttttttcg tcgaattgct tttactggct catcatcact attgattttt tcgtctgtat
    20521 ggataatcgt gatgtttttt tcatttgtta agtatttctt gatttctgct tcttttccgt
    20581 aaagtaagaa ttctacttct ggaaaatctt gctgcgctaa agccacgcct tctacaattg
    20641 cttgaggtgc gtgatctccg cccattgcat ctacggcaat tttcattgtt tccactcctt
    20701 tttccgtcat cagtataaag tatagcacag tctgctaatc gaaataacga ttatcctgat
    20761 tttctatttg gaggctttgc gctaaaggct gatactcagg aagcatctgc caatccttta
    20821 tctgccaaag ggctgcagcc tcttctctgg ctacctccaa aacatttcca tccgtcacca
    20881 agtcagcaat cgcaaattga ggcagaccag attgacggaa gccaaatact tcaccaggac
    20941 cacgtaattc cagatctttt tcgcttagta caaaaccatt ggtggtctct gtcatgattt
    21001 tcatccgttc cacacctagt tcatttttag gattagcaac taagatacag taggaagcat
    21061 cacttccgcg tccaacacgt ccacgaagtt gatgcagctg tgccaatcca aatcgatccg
    21121 catccatgat cagcatgacg gtcgcgttcg gcacgtttac ccctacttca attacagtag
    21181 tcgaaactaa tatctgcatc tggttgtctt tgaaggcttc catgattact tctttttcct
    21241 gattcttcat tttaccatgt aacaagccaa cttcatattt tggtgcaaat agttcacgaa
    21301 gtttttcata gatttccact gcatttttta catctaatgc ttctgattct tcaatcagcg
    21361 gacagatgac atacatttga tgtcctcttg ccagttcttt tttcgtccaa tccagtacac
    21421 tgtccaattg aggtgttctt acccagcgtg tttcgatcgg gatacgtcca gctggaagtt
    21481 catcaatgat agatacatcc atctcaccat atgctgtaat agctaatgtt ctaggaatcg
    21541 gagtcgctgt cataaataag acatctggtc gccagccttt ttcacgaagt actttccgct
    21601 ggttcacgcc aaaacggtgc tgctcatccg taataactag acctaaattc tgaaattcga
    21661 ctccatcttg gatcaatgca tgtgtgccga tcacgatgtt aatttcccct tgctctagtt
    21721 tttcaaggag ctcacgacgt tcttttgctt tcgttgaccc cgtaagcaat gcgatggtta
    21781 cttcattggg atcaaacagc tgttgcaagc tttccatatg ttgttgtgcc aagatttctg
    21841 taggcaccat caacgcacct tggaaacctg ctgtaacggt agcgtataac gcaatcgcgg
    21901 caaccactgt ttttccgcta cctacatctc cctgcaaaag tcgctgcata tgctgcggac
    21961 tgcgaagatc ccgacagatt tcattcgtta cacgtttttg tgcatttgtc agttcaaaag
    22021 gtagcttttg agtaaattct ttcaattttt ggacatcata aaggatctcc aaaccattcg
    22081 tttctgcttt ttctgctttt ttcaatcctt gaagacgcat ttgaaataaa aagaactctt
    22141 caaaaacgac acgtcgtttg gcttgatggt tttcttcgtg gtcttttgga aaatgcatac
    22201 ttcttacagc agaagctcgg tcaagcagtc gatatttctc cacaaggttg tttggcagat
    22261 tttcttcaat caatgtgccg tattcttgaa accctttttt gattagatca actaatgtcg
    22321 tttgacgaat tgatttgttg acgtgatata tcggcgaaaa gtcatccatt gtttgagaac
    22381 ctaaaatctt catcccgttc aatgctttac gctttgcatc ccatttaccg tatacggcga
    22441 tttcctccga taaaatgact ttttctttta gataaggttg attaaaaaaa gaaacgttga
    22501 acacatcatg ttcttgcatc atacgaaatt gcaatcggct ttttttataa ccaaatcgat
    22561 tcatcacagg aggcgagact acgattcctt tcaaagtaac cttttcttga tcttggatct
    22621 cgtttagatt tctttcctga atatcttcgt agcggaaagg ataataagtc agtaagtctt
    22681 caatcgtttg gatgcccaag ctggcaagac tagctgctct tttttctccg actcctgcca
    22741 gcatagtgat tggatcatct aaagaacgca taaatttttc ctccctccct taacaaaaaa
    22801 aggacttggt cagcgatcat acctgatcac taaccgaagg ccttttatga atgaatactg
    22861 tgttagaaag atattctgac acagaaatta ttcagcagaa aatagataag gatagacagg
    22921 ctgatctcct tggtggatct cgatttccaa atcaggatgt ttttctgtta gtttctcagc
    22981 aaattcattt gcttgttcaa ctgttccttc atcacctgcc aagatcgtta cgatctctgt
    23041 gtcctcattg atcatttgtt ctagtgtact taacgctgtt gctgtgatat ctgggttaga
    23101 gatcaggatt tttccatcga tcatgcccaa aaagtctcct tctttgatct ctacaccgtc
    23161 aattgaggta tctctgatcg cgtgagtgac ttgaccgctc acaacgctag acaacatttc
    23221 tgtcatcgtt tctttgtttt cttctaaaga agcttgatca ttgaatgaaa gcatagctgt
    23281 caatccttgg gaaatcgttc ttgaaggaac aactgctact gggatatctg ctacttctgc
    23341 tgcttgatca gctgccatga agatgttctt attgtttggc aagacaatca ctttatctgc
    23401 gtttacttct tgaatcgctt taacaatgtc ttccgtactt ggattcattg tttgcccacc
    23461 actgataaca taattagcgc ctaaactttc aaacagctct tggacgcctt taccagcagc
    23521 gatcgctacg ataccatacg ggatacgtgg tgtttctttt ttcgcaaact cgttcacttc
    23581 ttcatcgtgt tccaaaatcg tctcatgctg caagcgcatg ttgtcgactt tgattttgac
    23641 taatgaaccg aatttttgtc cataattcaa gacttctcct gggttttctg tatgcacgtg
    23701 gactttgatg atctcgtcat cattgaccac aagtaaagaa tctcctatac catctaaata
    23761 gttacggaat tcttcgtaat cgaatgtact atcgactgta ggtccttctc cgatttttac
    23821 catgatttct gtacagtaac caaaatggat atcttctgtc gccatttgac cttggacact
    23881 gcgatgatgt tctgcattga ccatatcatc catttctgct ggagaaggtt catatgtttc
    23941 atccgcttgg aattctccat tcaatgcact taaaaagccc tcataaacaa acagcagtcc
    24001 ttgccctcca ctgtctacga caccgacttc ttttaggact ggaagcaagt ctggagtttt
    24061 agctaaagca cgctttgccc cttttacaac agctgtcatt acttcaacga catcatcagt
    24121 actttttgct ttcttttctc cagctttcgc tgcttctcta gctactgtca aaatcgttcc
    24181 ttctactggt ttcattactg ctttatacgc agtttcaact ccatgagtga aggctgcagc
    24241 aagatcggaa gcatttaatg agacaacatc tggaatttgc ttagaaaatc cacgaaacaa
    24301 ttgagacaaa ataacgccgg aatttccgcg cgcacccatc agcaatcctt ttgaaagcat
    24361 tgctgctaat tctcctactt tttctgaggt tgaatctact actgccttag ctccactagt
    24421 catagataga ttcatatttg ttcccgtatc gccatctggc acagggaata catttaatga
    24481 attgacgtat tctgcattta cttgcagacg attcgcacct gcctggacca tttcctggaa
    24541 ctggcttgca cttatttctg ttacactcac ctagataatc ctccttcaga tcgactgctg
    24601 agttaccagc cagtctcctg cctagtctgg cagtacacga acaccttgta caaagacatt
    24661 aactgaattg gcagttacgc ctaataatgc ctctagattg tatttcactt tttcttggac
    24721 gttgcgggaa acttcagaga ttttcgtacc atagctgata atcgtataaa catctacagc
    24781 aataccatta tcttcttggc ggacaactac tcctcttgag taattttctt tacgcaaaat
    24841 ctcagtaata ttatctttaa tttgattttt gctggccatt cctacaacac cgaacacatc
    24901 tgtcgcggcg ccaccaacaa ctgttgcaat tacatcattg gtaatttcaa tggttccagc
    24961 tgacgtttga atttttacag ccatgaatga gcctccttaa cgggtaaaaa actcccattt
    25021 ttcttctatc atattttatc atagcgcaac ttgaaataaa aggcagttac ccaaagtact
    25081 caaaaacctt gcaaaatttc aggtataacc ttgtttaatt tacaattatt cttctcaatt
    25141 gtcaattgta tctatgaaaa tgtacttgca aatttccatt ctatatgata tgttagtctg
    25201 gtatgagctg aattattcta ttgctccaaa tcaaggcaaa ggaggaaatt accatggcaa
    25261 aagtttgtta ctttacaggt cgcaagacaa aaagtggaaa caaccgttca cacgctatga
    25321 actcaacaaa acgtactgtt aaacctaact tacaaaaagt tcgcgttatg gtcgacggaa
    25381 aacctaaaaa agtttgggtg tcaactcgtg ctttgaaatc aggaaaagtt gaacgcgttt
    25441 aattgaatga tacttggacc gttcagatag tgaacggttt tt
//
LOCUS       DAZULG010000027        25166 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000027 DAZULG010000000
VERSION     DAZULG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.027"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            516..659
                     /locus_tag="ACG9CY_001036"
     CDS             516..659
                     /locus_tag="ACG9CY_001036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4937562.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            705..1238
                     /locus_tag="ACG9CY_001037"
     CDS             705..1238
                     /locus_tag="ACG9CY_001037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937563.1"
                     /translation="MANEKATMTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQL
                     SWAAIWGKHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAR
                     EVGQQLGLTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGV
                     KRFGDGIHDHMGALFLA"
     gene            1382..2671
                     /locus_tag="ACG9CY_001038"
     CDS             1382..2671
                     /locus_tag="ACG9CY_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4937564.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            2756..3628
                     /locus_tag="ACG9CY_001039"
     CDS             2756..3628
                     /locus_tag="ACG9CY_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4937565.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            3618..4433
                     /locus_tag="ACG9CY_001040"
     CDS             3618..4433
                     /locus_tag="ACG9CY_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4937566.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            4449..5573
                     /locus_tag="ACG9CY_001041"
     CDS             4449..5573
                     /locus_tag="ACG9CY_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937567.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            5551..6354
                     /locus_tag="ACG9CY_001042"
     CDS             5551..6354
                     /locus_tag="ACG9CY_001042"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4937568.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            6814..7077
                     /locus_tag="ACG9CY_001043"
     CDS             6814..7077
                     /locus_tag="ACG9CY_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4937569.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            7061..7396
                     /locus_tag="ACG9CY_001044"
     CDS             7061..7396
                     /locus_tag="ACG9CY_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4937570.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(7575..8789)
                     /locus_tag="ACG9CY_001045"
                     /pseudo
     CDS             complement(7575..8789)
                     /locus_tag="ACG9CY_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            8990..10264
                     /locus_tag="ACG9CY_001046"
     CDS             8990..10264
                     /locus_tag="ACG9CY_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFS4937571.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(10647..11375)
                     /locus_tag="ACG9CY_001047"
     CDS             complement(10647..11375)
                     /locus_tag="ACG9CY_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4937572.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(11353..12927)
                     /locus_tag="ACG9CY_001048"
     CDS             complement(11353..12927)
                     /locus_tag="ACG9CY_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4937573.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            13156..14094
                     /locus_tag="ACG9CY_001049"
     CDS             13156..14094
                     /locus_tag="ACG9CY_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4937574.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            14106..15017
                     /locus_tag="ACG9CY_001050"
     CDS             14106..15017
                     /locus_tag="ACG9CY_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4937575.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            15041..16513
                     /locus_tag="ACG9CY_001051"
     CDS             15041..16513
                     /locus_tag="ACG9CY_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4937576.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            16564..17175
                     /locus_tag="ACG9CY_001052"
     CDS             16564..17175
                     /locus_tag="ACG9CY_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4937577.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            17205..19118
                     /locus_tag="ACG9CY_001053"
     CDS             17205..19118
                     /locus_tag="ACG9CY_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFS4937578.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            19246..22227
                     /locus_tag="ACG9CY_001054"
     CDS             19246..22227
                     /locus_tag="ACG9CY_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFS4937579.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            22224..23885
                     /locus_tag="ACG9CY_001055"
     CDS             22224..23885
                     /locus_tag="ACG9CY_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFS4937580.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            23925..24857
                     /locus_tag="ACG9CY_001056"
     CDS             23925..24857
                     /locus_tag="ACG9CY_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS4937581.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         8107 a         4053 c         5142 g         7864 t
ORIGIN      
        1 ctgtctgtga ggacagcttt ttttgtgggc taggcatagc aaaaaggggt agtgacagtc
       61 cactgggagg gtgcacatcg accaatcgct agagaaacgc aaggtggtat ttgtgagaag
      121 atgacggaag aaatcctgta tcaaagactt ggttcaacga gcagaaatct gatagtaagt
      181 ctcggtcatt caatgggaat aagccattga acaccttgat ggtatcctgt aagaaagaac
      241 ctcaaggact ttcagcaaga agaaggaata gctgaaaaca aaaaaaggtg tagtaggtta
      301 aaagcgggga atgtagaact acttaattgg tgaaactagc tagaagggca aaatcgacga
      361 ttcatgagtt cgtaggaagg agcatgcaag atttacaact agtttactga aaaaacgcag
      421 ggaatttaca agaaattaac aagaaattaa cgcttcatcg gtatagtgga cgtgtgggag
      481 aaaatcacgg aattaaagga ggggtcaaaa tgaccatgat cactgcacac tctggtagtg
      541 acaatacgga ggagaatagc tgggcatttc ttcgcttttg tttggatcgc cagctttgtt
      601 ttgaagtaga tgtgcgtcag caaacagatg gtacgctaat actagcccat gacaggtaag
      661 aagttgtccc agattcggta acccttgagc aggtgtttca agcagtggcc aatgagaagg
      721 caaccatgac cattaactgt gatttaaaag aagagggatt ggaaatggcc gttttttcgc
      781 tggcacaaac ctatcaaatc tggccacaaa ttgttcttag tggctatgta caattatctt
      841 gggctgcgat ttggggaaag catatatttt ataatattga aaatcagttc gaagggaaaa
      901 aacttgggga tattaccgaa gcagatttga tcgatacctt acagaaatta gcctatcaaa
      961 gcgttcaaat cgtcaatatt catgaaggat ggttaactaa gttggcccgt gaagtggggc
     1021 agcagctagg attgacattt tctgtatgga cagtcaatga actagaaacg attgagtacc
     1081 ttgcgcgctg tggtgtttat aatataacct cacgttgtgc atgggcctat ttatggaaga
     1141 aaggagcaca gaataaacta ttttataatc aaaacagagg agttaagaga tttggagatg
     1201 ggatacatga ccatatgggg gctttattct tagcttgatg gatatgtcca gtgaccctgt
     1261 accccgaaat gcacaaattt cgaattattt acagaaaatt aactagaaat ttacattggt
     1321 agttcataat atttcttgat tagaaaaatg gccataaaga aattaggagg aagttaatgt
     1381 tatgttaaag aaaaatcttg ttataggggt tgtgtctgtt ttaagtgtca tctctttggc
     1441 agcttgtggt agtgggaata ccaagacaac aaaaaaatcg gagggtcgtg taacgataga
     1501 gtattggcat gttaatgcgg ataccctagg tggaaaaaca gtgaatgaat tagtagataa
     1561 atacaatcaa tctcaagaca aagtgaaggt tgtagcaaaa tataatcctg atatgtataa
     1621 aggcttgatg caaaatttac aagcttctgt tgcctccaaa gaaataccgg acgtagtaca
     1681 agtgggatgg gcttttaaag attattttgc agaaaatttc aaatatacta atcctcaatc
     1741 tttagtagaa gaagtggatc cggataatgc tgattatttt aaagatcatt ttttagacaa
     1801 tatcatggca ctgtctgaaa aagatggtaa acaagtgggt attccttact ccattagtaa
     1861 tccggtttta tatttaaatc aagatatatt aaaagcagcg ggcttggatg aaaatggtcc
     1921 aaaaacttgg gaagaagttc aaactttttc taagataata aaagaaaaaa ctggaaagta
     1981 tggcgtgtat attcaagaac ctgctgataa ttgggcacaa caagcaattt tagaaagtaa
     2041 tggggcaagt gtaataaaag atggcaaagc tacctttgct tctaaagatg gtatcgctgc
     2101 ttatgaatta tatcaaagga tggtaaaaga ccagacggca ttacacacta cgtgggaaca
     2161 aggtgtgcaa agctttattg atggtaacgt cgcgatggtc tataccacta ttgctcaaag
     2221 ggacaacatt caaaaaaata gtacttttac tgctaaagca gttgagtcac caagttggga
     2281 agggaaaaaa gttaaactac ctgctggtgg cgcaatgtta gctgttacat cacaaacgga
     2341 taagaaaaag gaagcaactt gggatttctt aaaatatctt tatagtgttg aatctttagc
     2401 taaatggaca gaaggtactg gttatttacc accacgtaaa gatgttgcag acgcccaaaa
     2461 tggattaaaa gctttcttag ctgaaaatca gatgatgata ccagcaattg atcagatgga
     2521 taagatggtc agttggagtt catttcctgg agatgctgga ttagaagctg aacaaaaatt
     2581 gttggatatg cgggataaaa ttttatcagg taattccgtc aatgaaacga tgaaagctac
     2641 tcaaaaggaa attaatgagt tactcaaata agcaaagcaa gcagacgaca gcgcctgtaa
     2701 gaaaggaaaa acaaggtcgt ctgcttttga cataaggatg aggaggctgt cggaaatgaa
     2761 gcgctatgta ggttatttgt atctggcgcc agcaattatt ttgttgggcg tgtttgtttt
     2821 ttatccattg atttatacga tctatttgag tttttttgac tggaatatgg tggcccccac
     2881 taaagaattt gttggctttg ccaactatgt aaaggtgctg actgatccag tctttcataa
     2941 agtgttgaag aatactttgt cctataccgt tctatttgtc ttgctgagct gtgtgttccc
     3001 atatattaca tcctttttat taggaatagt tatagaaaaa gggcagaatt tgtataaacc
     3061 attatttttt attccagctg tgatttcttt ggtagtggcg gggatggttt ttacatggat
     3121 attaaatccg gtatcgggac caattgctgt cttgttggga aattttggtt taagactgcc
     3181 tatgtggatg aatttacaag gttgggtaat tgctgtgatt gcaggtatta ctgcttggaa
     3241 agtttttggt tataatttca ttgtcatttt tgcagcaatt gtgggaattg atcaagagat
     3301 gatcgaagca gctaagttgg atcgtgtacc catctggcgc atttttttta caattgtgtt
     3361 gcctatgagt agttcgacag gactatattt attggtgatg accattgttc aaggtctgca
     3421 gtatgtgttt actccgatta agatcatcac tcgtggtgga ccgaataatg gtagtgcaaa
     3481 cgtgatctat tatgcttatc aaaaggcttt tgaaatgtat aaagtcggag aatcttcagc
     3541 aatttcagtt ttaactttat taatatttgc tgttttctta ggggtaatat ttaaatttgt
     3601 ggaaaagggg gtttactatg aaaattaaac gctggttgcc acatctctta tttatgttca
     3661 tcataatcat tattatctat ccattattgt ttattatggc caattctttt aagccattga
     3721 aagatgccta tcagacgatt actagtttga taccacagcc gtttaccttg gaaaattttt
     3781 gctatttgtt cgaaaatctt ccaattatca aaattacgag aaatactttt tttattgcta
     3841 caattataac ggttgttaag ttaggcgtag ctttttttgc ggcatatagt tttacttatt
     3901 gccgaatcaa agggaagaaa atctggtact ttatcatcat tggtacgatg ttcattcctt
     3961 ttactgttgc gatgattcct aattatttgt tgattgcaaa acttaattta attgatacta
     4021 cttggggtgt gataatacca caatttgctg atgcgatggg cattttttta ttgacgcaaa
     4081 caatgcggga aattccagtg agccttctag atgtggcggc attggataac atcaaacaaa
     4141 caaccgtcat gagtaaaatt gtttttcctc ttactagaca tgcgattact tctaccggta
     4201 tttggttttt tattacctct tggaatgagt atgtttggcc ggtattaatt ttacggacag
     4261 tagataatta tactttgccg ttggcgatgc agacctatat tagttctgaa ggtgggacaa
     4321 attttaccgt agcaatggcg attagtttaa ttacaatgtt tgtaccgtta gtcttgtatg
     4381 caatttttca aaaatatatt atcgggactt ttgtttctgc aggtattaaa taaattggag
     4441 gacaaatcat gggagaaacg cttgaattaa gaaatatcgg aaaaaaatat ggagaacata
     4501 ccattttaaa agaaattgat ttagctgtgg ccgctggaga acgaattgtg ttactgggac
     4561 catctggttc aggaaaatct actttgctga gaatgattgc tggtttggaa aaaattacga
     4621 gtggagagct ttttttaggt acacaactag caaataagct accttgtgga gaacgagaga
     4681 tcgcaatggt ttttcaaaat tatgctttat atcctcacat gactgtaaag gagaatatta
     4741 tttttgcatt aagggcgaat aaaattaaga cagctgacat tcagcaccgt ttggaagaag
     4801 ctttgaagat gttaggttta acgccatacc aagatcgact tcctaaagat ttgtcaggag
     4861 ggcaacggca gcgtgtagca ttggctcgtg cggtggttaa acggagtaat tattttttat
     4921 tagatgaacc gttatcaaat ttggatgtgc gtttacgatt ggatgcacga aaagaattgg
     4981 taaaaattca tcaacgttat aaacaaactt ttgtctacgt cactcatgat caaattgaag
     5041 cgatgacgtt agctgaccgg attgttttat tgaatgaagg tagaattcag atgattgata
     5101 cgccgaaaaa tgtttatacg aaacctaaaa atacttttac tgcaaatttt attggttcgc
     5161 caggcatgaa tattatgcca gttacttatg atcaaggaac attaaaggta gccagtcaat
     5221 ttttaacatt gaatccagct tggcaaaaac aacttcttca ggttagtggc tcattgtatt
     5281 taggcataag accagaacac gtaacctttt caaaagaaac aagcgattta cacggtcaag
     5341 tgaagtatcg tgagctatta ggtcaaagtt atgcgtacac cgttaaagtc gatggtatag
     5401 aaatgattgt ccttaatgaa aaacctactt ggcagattgg tgatgcagtc aatctccgca
     5461 tagaacctga aagaatgcat ttttttgatt atcagacgca agaaaattta ggatatcctg
     5521 attattttat tgaagaaaga gggaatagcc atgcgctttt tgcatctctc tgatatccat
     5581 tttagagaaa actatcaaac taacttgttt actgctgaac tatttccaac aattaaaaat
     5641 ccacagttgg cattggaaaa attgttacaa gaactctcgt taacagtgta cgattttatt
     5701 ctattaacag gtgatttagt acatgaaggg gggatttctg attatcaagt cttacggaaa
     5761 atgttggatc aatatttccc aaccacacct tactattttt gtcgtgggaa tcatgatcgt
     5821 cgcagtgttt tctttgaagg aatgagtgtt gaaagcaatg aaacgggaga gtatatccat
     5881 tgtacgaatc aaaatgggtt acgaattatt tccttggatt ctgctcaaga tacccagcac
     5941 gaaggaaaaa tatcgatagg acagatgatt caattacaag agtggttggg tacacctgct
     6001 gaaaagggaa cgattttatt ggttcatcat ccattgtcgt gggaagatcc atctattgta
     6061 actgaagttc cggaaggttt tgctgaattg attgccgaaa gtgatatttt aggtattttt
     6121 gtaggacata tccatcaagg tagcagtgct gaatttgctg gtaaaaaaca gtttatggct
     6181 gaatctttat catttggtgt agatgaatac ccagactatt ctctttttac caatagaaca
     6241 ggattcacta gtggcagttt aattgacgga aaaattttta cttatcagca ttatttaact
     6301 ccacatcaaa tagaaatagg gcgcatgaaa aaaccaatta ctggtaatca ttaaaaatta
     6361 ttgattaaaa ataaagactt cattcacttt ttagccgtgg tgctcaatgt ctggttccac
     6421 ttgaagaact tggaaaatcg ctatcaataa accactatcc ctccaattac aaaaagggtg
     6481 gagaaagttt tccaagaacc atatcggtct tcgtggttgt gccattcgtt ttttaccaga
     6541 gcataccaca tctttgtgga gttttcagac acgtcctagt taatttggtt cttttaggag
     6601 tcttttcctg ttctgtgtgt cgttgagtgt tcgcttcaat tatcgctgtg gcggattttg
     6661 aaacttttct atctatcaag gccatttagc actatatcct ttccttatat ttagataaat
     6721 acactgcatt tcaaggagtt caaaagtagt tgtattaatt ccatttgaac tctttccgtg
     6781 atataatgtg tttgtcagaa agaggtggag aaaatggcta agttacaaat ttctatggac
     6841 gaaaaattga aaaatgaagc agaaagcatt attgatgaac taggcatgag ccccaaaaca
     6901 gccgtcaccg ttttttataa gcaaattgtt gagcaaggaa aaatcccatt ttctattgaa
     6961 ttgtcggaaa aaagtaaacg aatccataat attcaaagat tagcggaaaa attgcctgtc
     7021 caaaaactag acactgacga aaaaattgaa gaatggttca atgaagatga atactaatga
     7081 tatagcaacc gtctacgttg ctttcacagc tgcagagggc ggtaaacgcc ggcctgtcta
     7141 tattctaaaa aatggactgg acacaatttc gtttctgtca attacttcaa aatatcatac
     7201 taaatccgat aaaataaagc tgaaatacgt tgaaataagg gattggaaac aggcaggact
     7261 aaaaaaagaa tcttggattg acgtaggaac tctaaacgaa ttacccaaaa acaacaaaaa
     7321 ggtcaaatgg aaaaaaattg gggaattaag tccaagcgat ttacagcgac tggaagaacg
     7381 catggaagaa ttataataat actaaaaaga agggctcttt gtcaataagg actgatgggt
     7441 tgtacaaaat caaatctggg tcagaatgaa cctcattctg gctcagattt ttggctcttt
     7501 gtcaataagg actgatgagt tgtgcaaaat caaatccggg tcagaatgaa ccacattctg
     7561 gttcagattt tttgttatct tacattgatt aattgattga tcaaaaagat tctaattctc
     7621 atgttctcga atgatttaaa tccgtaggat actcgtttca ttgtctttat gtgggtattc
     7681 ttcgcttcaa tttttccatt ggaataagga tagatcattg cgctggtgat accttcttca
     7741 taggtcagaa ggttttgaag cttctcccga aagccgtcat ctaacgtttc gggaagttct
     7801 gccaataagg agaaaaataa gtcagggtct ttgtctcgaa aggcttcgac taattcatga
     7861 aaaaagggat acgcctcctt tagaggcgca gaaaactcaa gcaatcgatc aatcatcatt
     7921 gcttcagtga gaaatgggta ttttggtgct cggaaacttt tccatgtttt gtactcataa
     7981 tggttgatgc ttgcacgatt ttttagcaga aagcgccagt tctttttcag tttttctgcc
     8041 tgacttttct gtcctgcttt acgaagctca ttcatttcac ggatacgcaa ctcattgagt
     8101 gcctgattca tgtgtttgac aatatgaaac cgatcaatca ctactttcgc atttggcaga
     8161 acacgtttgg taagctggaa gtaagcggcg ttcatgtctg tgactaagaa ttctacttct
     8221 tctggattgg tgcagcctaa gaaatagctt gttaatcgag gtaatttacg cgtaggcaag
     8281 acagcaatta agtttcctgt ttcaccatct gcgcaaataa agctcatttt atcttctatg
     8341 gaagcgtgcg aacgaaattc atcaaccatc aatactctgg ggagaatctt cttagaaggc
     8401 tttggtaaat agcttttaaa ctctttcaat gtacgagtaa cggtagtcaa agatacctga
     8461 caacttttcg caataaaaga caaagatact ttttcagtca gtaaagaagc aattttatat
     8521 cgaacatgat ctgcgattga atggctgagt cggacaaaat aactttgagc cgtccaatgg
     8581 gtacgacagt ttttacaggt atagcgttgc ttttttaggc gcgtaaccaa aggcatatga
     8641 ttgtattgtt caaaacggac aatcgtttcc ttcttcccat ttttcactat aattgctttc
     8701 ccgtttccat ctaccacagt agaaccacaa cttctacaag cacgaggagt aggcgagaga
     8761 acagcatcga cgaccaacgt ctttttcttg gctctttgtc aaataggact gatagagtga
     8821 tataagaaca tctaaggtag aattgatttt gcttctataa tattttgtgt tggtggaaaa
     8881 tcgaaggcga ttttctacta acactttttc ttcgtttcaa ctcaaaaaga gactcacttt
     8941 tggtaaaatt tagtcgacca aaacgtcatc ctaccaaagg agtctctcaa tggataatca
     9001 tactagaaaa ttactcggat taacagataa acatttattt tttgatgaag aatggctgat
     9061 tgaaaaagaa tataaaggcg ttcaagcgca gtttattaaa ggaaaattgg atgactctcc
     9121 ttcctattgt gaacactgtc gatcggttcg tattatccgc aacggttcct atacgacacg
     9181 cactcagata ttgaaagtca aagaaaagtt aactatttta gaattaaaac gtacccgttt
     9241 tctctgccat gattgtggaa ggaccttttc tgcgaaaacg gacttagttg atgaacatca
     9301 tcaactaaca aaggaactga agcaggcaat tctcatggaa ctgtacgaaa atcaatcacg
     9361 taaactaatc gctaagaagt acttcgtctc ggatgggacg gtgacacgga tcttgcgtga
     9421 agcaacgaag cattaccagt cacgtatgaa cttccttccg acggtgttgt gtatggatga
     9481 gttcaaatca atgaagtctg tttctggttc catgagtttt atttgtgttg atgggacaac
     9541 ccatcagcta tttacgatcc ttgaagatcg cagactttac aaactaactc aatacttcct
     9601 gcactttcct agaaaagccc gtttaaaggt caagtaccta gtgatggaca tgaatgccag
     9661 ctattgtcag ttgcttaaaa cagtctttcc aaatgcggag atcgtaactg accgctttca
     9721 tattgtccaa cacatcaatc gcagcttcaa tcaattacgt gtccagatca tgaatcgttt
     9781 tcgaacctca cactctgaaa atcagaagaa gtaccgtcgg ctcaagcgat attggaaact
     9841 cttactcaaa gattccacga catgggagcc actcaaacgg cattatcatc gactgtttaa
     9901 acgaccaatt agtcaaacag aaatcgttga tgagctgtta agctacaacg aagaattgcg
     9961 aactgcgtat catttttgtc aattattacg ctattatttc gttaaacgag gtacggaagg
    10021 ctttcaagaa acccttaaaa caatttcatc tacgttacct cagtcgttta aaaagaaatt
    10081 cacgatcttc cattgctacc aatcgggtat ttcaaatgcc tttaaggtgt cccactcaaa
    10141 cggcgtcact gaggggctga ataacaagat caagctgatt aaacgaattg cgtttggtta
    10201 tcgcaacttt tataactttc gggcacggat ctatttacaa caaggtctga tctttgaaaa
    10261 ctaaaaaaat atgcggcgca cagaacgcgc cgcatatcgg actaagtttt attcaaattt
    10321 gtcttcacca acacgatttg acgaagagcc tatttaaata actacatcca tttattctat
    10381 cttctaaaat agcctttttc atttcagtta aatagttcag cgccattttt gcactcgact
    10441 tgcatagtag ttttttccat attcctcaac ctcagcgggg gttgaagaca taaggaaaat
    10501 cctatgcgtt atgctcaact ggatctgatt aaatattcta atgtattttt ctctaccatc
    10561 attaaatggt taatagccaa aaatcaccac ttttcaatgg ctccttgttg tataagtata
    10621 actagaagat gaacacgtct gagaaattat agaagctttt tacggtaatt actggggctg
    10681 tgtccagtag tttttttgaa gagaacagaa aaatagctca cgtgatctat tcctactttt
    10741 tgactaattt catcaataga taagaatgtg tctttcagta gagcacttgc tcttttgatt
    10801 cgaaagtcta gcaagtaatc atgaaaggaa taacctgttt cttttttgaa ggcccgtcct
    10861 aaataagcgg gatggtaatg gaattgtgta gctacatctt tgagcgtaag cttttcacta
    10921 taattgtttt gaacgtaatc aatcacttgt tgaattgact cattaaaaga atcatcagtt
    10981 agagagtttt gattagctgc tgatttacgc attttttctt cttgtactaa cttaactaaa
    11041 taaggaaaat caatgggttt cgttatgtaa cttttaatag ataaatcaat cccctttttt
    11101 gcatactgaa aattttggtg ggcactaatg acaatactag taaagtagcg attggatgtt
    11161 tgttgcaaag attcgattaa ttcgagtcca gaaattttcc ccatcgtaat atctgtgata
    11221 agtaaatcaa agcgctggtt ttgcaggttt ttaaatgctt cgtctccgtc tgtgtagcag
    11281 tgatcgataa tgatgtcggg gactttcttc aaaagataat gccttatgcc ttctacaatt
    11341 gtacaatcat cgtcaacaat tgcaattctc aacatgcata ttcccccctt tttgtatttt
    11401 gatagtaatc gttgttccga cgtgtagttg gctttctata acaaaacgtg tatctccagg
    11461 atagagatgc tttatacgtc gaaaagaatt tgagatgccg atattctcac tataaggaat
    11521 atcatcatta agtttttgtt gaatatgctc cagcttgtct tggtccattc ctttcccatt
    11581 ttcaacgatt tttaacacat aatggttggt tgtttcagtt gcatagacag tcaaacgatt
    11641 ttcctttttt gatgaatcga agccgtgttt aatataattt tcaacaaatg gctgtaaaga
    11701 aagttttggt aacattaatt gatcaatatg atcaatcagc tgatagtcaa agttatgtcg
    11761 ataacgacgt tgatgtagtt gcaaggagag gtcgatatta attaattcct ctttcaaatc
    11821 aattatgtcg ggctgatatg tattattgcg atacaacttg ctcaataaat atatactgtc
    11881 gctgatttct tcgtcatcat tcaatatggc agtcattcga ataaattcca aattattaaa
    11941 tagaaaatga ggctcgattt gatattgaag tgctgcatac tttgcttctt gcttcaacca
    12001 attattttca taaagtgttt tttgatatgt agcattatct aaataaaact gattgatatg
    12061 catagcgatt tctttcaaat caccgacttt gttttcgata ttgattgact tgtagtctcc
    12121 tgtagatgaa tgatttaagg aagtaatgat ttcctgatac tgctggatat agttacgaaa
    12181 acttctattt aataattgat gaatagcaag aataaacaaa ctcattaata agaaaatcat
    12241 gattgccaga cctaataagg tttgtgacga ataatttgga ttttcatata cttgtaatgt
    12301 gtggttaccg atagctatct gttgcgtttt tttccattta gaaggcgaga tgtcttcaga
    12361 taaattgacg gaatcaatac cattagacaa agaataattg taatgtgttg gaaagagact
    12421 cttttttagc gcgttgatcg aaacgtctag aatcaatgta gcatcgggca tgtcctcact
    12481 aatgaaactt aatgaacgaa taaaagaaat tttttcgttt tttgtaggtg cgtaggcact
    12541 taagtatttt tcgtaatcta atgagataga tatattttta acttcatcat cttgcacaag
    12601 tttttttaag ttatctacta aataggtagg ggagcgatcc tttgacaaaa tatcttccat
    12661 gtattcagca ggtgatgaga ggtagtagtc attaaggttt tcttggaaat cataattttg
    12721 atagatctct gtcacgatac cggagagaat acgattatat gtttccatat agtgggtgat
    12781 ttgctgaatg ttttgcaggt tttgattttt ttctttttca atcacattga tataggaaaa
    12841 gaacaatgtg aaacaggcaa gtaaagtaat aaaaaaaatc aatatgtatg taaaacgcat
    12901 aataactttt gataaatcgt ttttcattca aagtcctcat tttgataatc tctaatttta
    12961 tgaatcatta tagcacatat tacaagtgat gtaaacgctt tgcaaacagg gctacttatg
    13021 attgtgaata cagtttccgt ttaaaaacta ttctattgta gaggtttaat aaactaaaaa
    13081 taatgtataa aaaattaatg taagcgatta accaagtggg tataatcaag gaaacgctga
    13141 gagaggagag acaaaatgaa gctaaagaaa gggttattag gaaatctaaa aaaaaaccga
    13201 gcattgttta ttatgctttt accaggtgcg ttatggttat ttatattttg ctatatacca
    13261 atgtttggag ttgtaatcgc ttttaaagat ttcagaattt catcaagggg attctttaca
    13321 agtttgttca acagtccttg ggttggctgg gaaaatttca aatatctctt tgcttcgcaa
    13381 gatgcttgga tcattactcg caatacggtt gtttataacg ttgtaggtat tttgctaagt
    13441 actttttgta cagtgtcatt agcaatcatt ctgagccaaa tggcgaacaa acggttattg
    13501 aaaaagatgc agacatttat gatttttcct aattttttat catgggtagt catcagttac
    13561 tttgtttacg catttttgag tacggataaa ggattaatta atattttact aagcaatttt
    13621 ggtgtggaac ccataaattg gtatagtgaa ccgaagtact ggccttttat tattgttttt
    13681 acgagtttat ggaaaggcgt aggctatgga tccatcattt attacgcgtc aattatgggg
    13741 atcgatacta gttattatga agctgcttta gttgatggtg ccagtaaatg gcaacagata
    13801 aggaatgtag tcttgccaca tttaagatcg attattgtca ttatgttgat tttgtctgtt
    13861 ggtagtattt ttcgagctga ttttggattg ttttatcaat tgccaagaaa ttcgggggct
    13921 ctttatgatg tgacgaatgt attagatact tatgtctacc gtggattaat gaatagcgga
    13981 gatatcggaa tgtctacggc agcgggatta tatcagtcat cagttggctt tgtcttgatt
    14041 ttgattacta ataagatagt caacaagtta gatcctagtt cagcactatt ttaggaggaa
    14101 tcaaaatgat atcaaaatta aaacggcaga tgtacttttc gctgccagtc gaacggtttt
    14161 tcacaatatt actaatctta tttactctta gctgtatttt tccctttatt ttcgtaatcg
    14221 tcatttcatt aacagatgaa aaagcattac aactcaacgg ttacgaatta ttgccggctc
    14281 aatggagcac tgatgcatat caatacttaa tacaagatgg cggacagtta cttcggtcat
    14341 taggcgttac aattatgatt acagtgattg gaacgttgat tactgttttt atgacgggaa
    14401 cctatgctta tgttttatca agagcctctt tcccttaccg gaagttcttt actttttatc
    14461 ttttttttac catgttattt gcgggaggga tggttccttc ttacttagtg atgacgaaga
    14521 tgcttggctt aaaaaataca atttgggctt tgattctacc tcttgctttt agcccataca
    14581 atgtcattat tttaaggaca ttctttaaga agtcgattcc agaatcgatt atcgaatccg
    14641 ctaaaatgga tggctgtagt gagtttcgag tttttttcca aatcgttttg cctttagcga
    14701 tcccaggggt tgcaacaata ggtttatttt caagcttagg ttattggaat gactggttca
    14761 acgcattgct gtatattgac accaacaaat tgattccatt gcaatatttg ttaatgaaga
    14821 ttcaaaattc aatggaattc ttagcaaata ataatgacat tacattagcg cagcaacaag
    14881 caattcaaaa tagccttcct caagaatcaa cacgaatggc aatggtcgta gtagctactt
    14941 taccgattgc aattgtttat ccgttcttcc aaaaatactt tgttcaagga ttgacgatcg
    15001 gtggagtgaa agaataaaaa agaataggag atgttaaaag atgaaaaaga acatgcagaa
    15061 atatttgatt ggtggtgctt ctgtactaac ccttggccta ttaggtgctt gtggtagcag
    15121 taatggcgaa gcaaataatg aaacaggaac cacaaaagtt acttggtatc aggttggaga
    15181 agaacccaaa gattttgatg aggtcatgac aaaagtcaac gaaaaattaa aggaagaaat
    15241 caatgtagag ttggatatgc ggctgattcc ggacggggat tatcagcaaa agcttggtgt
    15301 gatgatcaat tcaggagaag aatatgatat ttgtttcgtc aatggcacag attatgtgaa
    15361 ttatgggaac aaaggtgctt tcattagtct caacgatcat ttggataaag agttgaaaga
    15421 ttatgcagct gaattgaatg aaggatttat tgaaggtggg gcaattaatg gcgaaactta
    15481 tggattgcca gtcaataacg atattgccac aacggaatat tggaccttta ataaagattt
    15541 ggtagatcaa taccaattgg atgtagagaa tgttgaaggt gtagaaacat tggctccagt
    15601 attagaaaag tttgcagaaa aaaatactga tacgaatatt acgccaatcg gtatgacccg
    15661 taccagtgtc tgtattcctt atgacaatat tatcggtggt ggtgtaccgc tagcggttgc
    15721 atttgaaggc gatacacaaa cagttgttaa tacttatgag actccagaaa tgatgactta
    15781 tttgaaggct atgcatgagt tttataaaaa aggatatatt ttgaaagatg cagccactgt
    15841 tacgacagat ccatttcgaa ttgatagtaa aaactggttt ttaggtcgta actcaagtgg
    15901 ttatcaacca ttagcacaaa aaaaattaga agctctagcg ggacatggat taattttaaa
    15961 accagctgga aaagctttta aaacgactgg atttgcaaga gtggcaatgc atgcggtatc
    16021 tgcttcaagt aaacatccgg aagcagcctt gaaattgttg aattatgtga ataccaatca
    16081 agaagtagcg gatttaatgg tttatggaat tgaaggaaaa cactatgatc gcaatgaaga
    16141 tggttcgatt actttaacaa actctgatga ttattatcaa ggcgcttggg cattggtaaa
    16201 tacttatcta gtttcaaaaa cttcaccgat tacagaagaa agtaaaaaac tagaagaaga
    16261 aagtaaaaag tttcaaaatg aagcaccgat gtctcctatt ttaggtttct cttttgacgc
    16321 ttctgatttt cgaacagaaa ttgcaagtgt acaaaatatc gtgtcacaat atgaagtact
    16381 tttaagctcg ggaacagttg accctgaaca atatgtacca gagttgttag aaaaactaga
    16441 aaaagctgga ttggataaaa ttcaggcatc tgctcaagaa caatacgatg aatggcgaaa
    16501 atctaacaaa taatttttta ctaaatgaaa agaatagact aagcagttag tggggggatt
    16561 caaatgaacg aaaacaaatg gaccaaagga tttaattggg tttttcaatt gttgttacta
    16621 aactttactt attttatact aatttgttta ggatttggaa ttttaggttt tttccctgct
    16681 acgatagctt tgattgctgg cgttgtgaat cgaaaaaaat atggcttgca agagtctgtg
    16741 aagttcatga gtaaaatcta taagaaaaat tttgttagat ataatctata ttgctggtca
    16801 gcaactttgt tgttggccgg caatattact acgatctggc tttgttggca gcaaagcgca
    16861 ccattgtttt atttattaag tacattatta tcggtaatta cactgttcct agtctttgca
    16921 atgttgaatt gggtcgtgat caaaagtctt gggttgtgga tgatgggaga ccgactgatt
    16981 gactatttgc ttttcacagc aacgatgttg ccaacggtag tcatgcaagg cttggtgctt
    17041 ggaatttatt tccgaatatt gatgattttg cctgtgattt ttctgtttgg cggaacagct
    17101 gtaacggtaa tattgattag taaattaact gaaagagcgt tagcaaagtt gccttgtcag
    17161 atacaatcgc actaattttt tcacaaaaag gagtatttct ttctatgaag ttaactgata
    17221 aacaagtagc taatatagcc tttatccagc aacttgagat tccattgaca caaaaagatg
    17281 aagaacaaat agacttgatt tttactgaca caaaggctaa tgtgcgacaa atcaagatta
    17341 ctaaaaatga gcagcaacga atttgcttgt cttattcaca tgaagacttg ctgtatcgag
    17401 gattgtttat ctgccttcca cgttttttag ctcaaggagg agagatgcca tacattgaag
    17461 aaacctcgat cattgagacc agttattcat tagatgcttc tcgtaatggc gtgatgaata
    17521 aagagactct aaaaaaattc atttcttact tagcgttgat ggggtacaac acattgtata
    17581 tttacacaga agatacctat gaagtagaag accttccata ttttggttac cttcgcggag
    17641 cgtatacaaa agaagaagtg aatgaaattg ttgaatttgc ttctggtttt ggcatagaag
    17701 tagttccatc gatccagacc cttgctcact tgacccaatt tttgaagtgg tatgctcaac
    17761 aagaactgat ggatgatcaa aatactctac tgatcggtga agaaaaggtt tatgcggcaa
    17821 tcgaaaagat gattattgcc tgcaaagaaa tgtatcagac agagcgtatt catttgggaa
    17881 tggatgaagc ctatcaagca ggtctgggac gatatttaag cctgtatggg tattgtgatc
    17941 ggacaacttt aatgattcaa catatggacc gtgtgttatc aattgttgat aaatataaga
    18001 tgaaaccaat gatttggagt gatttcattt ataagctatt agatgaaacc ggtaaagata
    18061 ggtattacaa tcctgatttt aaactcaaag cattagcagc taaaggatta ccagcaggat
    18121 taacctatgt tcattgggac tatggttcag atgaagtttc ctcctatgag aaagtaattg
    18181 ataaccattt gaaattttgt gacattgatc attacgtttt agctagtggt gcccatatct
    18241 gggggagact agccccgaat catgggaaaa cagaagcaat tgtttctgca gctgtaaaag
    18301 cttctaaaaa taaaggtgta cgttctgtga tgttaactac ttggggagat gaagggcagg
    18361 aaacagagca ttggcattcc ttaattgggg cattaaaatt agcagattat gtctattctg
    18421 aagaagagtg gaatgatttt gaggaaatct acgatcgttt attaggaaca ggtagttatc
    18481 aattaatgtc cgaattacga tatttcgatg aagtggatac cattacaaat aacaacttac
    18541 aaatggcttc cgtgagtaaa ctgctacttt ggcaagatcc gttagttggt atatatgact
    18601 ttcatgtagc cgctcataac aaaattgcca ctagaacaat tggtacttat tatcatgagt
    18661 tagctcagaa attagatatg aataaaaatt ggaatccaaa acaaccaatt ttaacaacta
    18721 ttcgtcaacg atatatactt ttagctgaaa ttttagcaca aaaggcagaa ctaggcatct
    18781 atttacaaca ctgctgtctg gtagaagacc ttgcaggatt aatggaatta gcgaatcggt
    18841 gtgaagcact agccaatcga gtaaatgaat tggcatctat tcatgaaata atttggtatc
    18901 aaacatacaa aacctatggt tgggaagtat tagaacaacg atatgctgca ttagctcatc
    18961 gtctgaaaac cgttgcgcgt cgtattaaaa atcatcttca ggggcaagat gccctagagg
    19021 agcttttgga agaacgatta ccgttttgtc aatatcaagt gccgcttgaa atttctggct
    19081 ttaattatcg gcaaagctcg gtgtcaggct acaattaacg aaggaatctt cccgttgaac
    19141 ggaccacttg ctcatctaat gggaaagtgg ttcgttttct attcataagt agcttaccac
    19201 taactctttt attaagaaga gaaaaaaagt aggaagtgag aaaatatgtt atcggactat
    19261 tatcaaaatc tgtcagttat aagtgtcaat acagaaccat tacgtaatta ttatattcct
    19321 tatgcaagta aagaagaagc aaaacaagag ttagaaaggg aaaaaacgag ccagtttatc
    19381 gatctcaatg gtacatggga ttttcagtat tatccttctg tccaacagtt accggaaaat
    19441 ttttttcaag aggcctttac tgaaagctat gatagaattt ctgttcctgg agtttggcaa
    19501 acagagggat ttgacttacc tcagtatatc aatgtatctt atccaattcc ttttgatccg
    19561 ccttatgtac ctaatgatac cccttgtggt gtatatcggc gtcagtttca aattgagcaa
    19621 acagatgcac gcttttactt aaatctagaa ggagcagata gtaattgttt tgtttggata
    19681 aatagaaact ttgtaggcta tgaccaagtg agtcatagta catctgaatt tgatgtgacc
    19741 aagtatttac aggttggggt taatgaaatt caaattctag tgatgaagtg gtgtgatggt
    19801 acctactttg aagatcagga taagtggcga atgagcggtc tatttcgtcc agtgtatctt
    19861 cttcaacgac caactatgca tctgacaagt tatcaactac ggacgttgac tgatcctaaa
    19921 aaaggaagcg gaacattaat ggtttctttt caacgattta ctcctattga agtatcgtta
    19981 cctatttctt acgaattatt ccatcctgat ggacgtctca tagcgaaagg ggagtgttac
    20041 tcggaacaac aaataaattt ggatgttgaa gattgccttt tttggacagc agaaacgcct
    20101 aaattatata cattactttt aacatgtaat ggtgaatatt ttaaagaaaa agttggcttt
    20161 cgagcactga cgatcaagaa tcgtaagtta ttgctgaacg gaacacctat taaattatat
    20221 ggagtcaact atcatgacgt gaatcagcaa ggcacgccag tgatgacccg agagcagtta
    20281 cgtgcagatt tgttattaat gaaaaaatta aatgtgaata ctgtacgaac ggcacattac
    20341 cctaaatcac cagattttta tgaattggcg gatgagattg gtctgtatgt aatcaatgaa
    20401 gcagatttgg agtgtcatgg tgtggttaat ctatatgata ctgaagcgaa ttatcaacta
    20461 attgctaatg atccgacatt cgaaaagccg attatggacc gagtgatccg ttctattctt
    20521 ccttatcaaa atcgaagctc tatcattatg tggtctatgg ggaatgaatc tggttatgga
    20581 gtaaattttg aaaaagctca agctttagca cggaaattgg atgattcacg gttgattcat
    20641 aatgaacgct cgattgaacc agttattgac caagtgaatg attttagtaa cttggatgtt
    20701 attagtcaaa tgtatccccc actggaagat gttcatcgtc attgtcaaag aagtgatact
    20761 gaaaaacctt tttttatgtg tgaatatagc cacgctatgg gcaacggtcc tggagatctt
    20821 aaaacgtact tcgatttgat tcaacagtat gattcctttc ttggcggttg tgtctgggag
    20881 tggactgatc atgcaatcaa tagaggaact ttagaaacgc catcttattt atacggtggt
    20941 gattttggtg aaaaatatca cgacggcaat ttttgcgtag atggtttatt gaccccagat
    21001 cggcaattaa cagtaggtgc attggagtac cgtaatattc attcgcctat tcaactgatt
    21061 gctgtagatg aggagctgca agaagtaacc ttgagcaata taactgcttt tacagccggt
    21121 tcagcattta acctttctct tgaatggtat atagatggaa aaaaaacagt ttatgaagta
    21181 atgaatcctt tcatacttga gcctggcaaa agttgttcat tgcccatcac agtacctaca
    21241 acgattcatg gtgctcatac ggttgctgtt tcgctatctg tttataaaaa tgatgagctt
    21301 tatggacagc aacagctctt tcttaaaaaa gaaaaaatga gattgccaag aactgctcat
    21361 cataatgatg caccacaaat ttcagtaaaa ggcacagagc tgataatcaa aggagaatcc
    21421 tttatttata tatatcaatt agcaaaagct gcgttcactt ctgtaaagat caatcaaaag
    21481 gaacttttgg aacgtcctac tcaattttca atttggcgtg caccaacgga taatgatcgt
    21541 aacttagtaa acaaatggaa agcagctgga tatgctgaaa tgtctttaga aatcagaaaa
    21601 aaagactgga aattcgtaga tgatcatctc ctgatcgaaa cgtcttatcg aggagttatt
    21661 ccaggattac agccaatttt tacaatgcac atacagttga aaataaatgg tgcaggcgta
    21721 atcaaccaaa aagtatcagt ggagaaaaat ccgatttttc cagatttccc tcgctttggg
    21781 atggactggc cgttgaatga tactttttcg aaggtttctt attgtggcta tgggccgtat
    21841 gaaagttatc ctgataagca ctatgctgcg atgtttggta tttatgagat ggagattgag
    21901 gaatttgatc agccatatat caaaccacaa gaatatggta accgctggca atgtgattgg
    21961 gtatcaatca aaaatcatca aggatctacc atgacggttt atggaaactc taatttttct
    22021 gctctgcctt atagtcaagc tcaattaacc gaaaagtcac accattatga attaataaaa
    22081 gaaaatagaa cttggttcaa tttaagttat aaacaaaatg gtatcggctc acacagttgc
    22141 ggtccttatt tagaagatcg tttccgtttt atggaagata cttttgactg gaatatagtg
    22201 attactttca aaggagaaaa tagatgaaag aaacaaagat caatcaagtc aaaaaaaaat
    22261 tatctcgtta tcaagaagtt gtttatcgaa accattgctt aaaatggtca aaaaataagc
    22321 tgaatgttcg ttttaatgga ttattttatc gtttcgaaca gttagaagaa gtctggaaac
    22381 cggtaacgaa tttccaatca tgtcctgtac agctcataaa tgatccaaaa gaaacatacg
    22441 atttatgtat aacacacctt tataaccagc atttgaaaaa tgacggattt tatttaaagg
    22501 ttactaatga caatcaactt acgattgaat cagcaaacct tcgagggttt caatatgcta
    22561 tggaagcctt attagagagt tttttttcgg taggagatca attattatta ccggaattga
    22621 ttctgaaaca tgaaccatca gtgaaaattc gaggaatcat tgaagggttt tatggtatcc
    22681 cctggacaca tgaaatgcgc ctctctcttt tttcatatat gaaagataac caattgaata
    22741 cttttatgta tgcccctaaa gatgatgaat tattacgtaa aaaatggcgt gaactctacg
    22801 atgcgcaaga attagacaaa tttaaggagc ttttatctgc tgctgaggct agcaatatcg
    22861 atttttggta cttaattagt cctggaaatg atattgatat tacgtgtgaa gaagacattc
    22921 aagttcttct gaaaaagtta gaacaactga ttgagcttgg cgtatttcaa tttggtctat
    22981 taatggatga tattgactat caattaaaag gtgctgctaa acgccgtttt cgagagccag
    23041 cattcgctca tgcctattta gtgaatcgag tagaggaata tcttagcact cggttgtcca
    23101 atcatgaatt agtcatttgt cctacagaat atgataatcg tcatggttct cgctatttag
    23161 caacgttaag tcaagaaata ccagaacaat taccgttctt ttggactggc ccaagcacgt
    23221 tagcggcgtc aatttctaca gaagagctgc aggaaatggc ttctgtctat caacggccga
    23281 tgctgatttg ggataatatt ccggtaaatg attatttaga ggataaagaa ctattgttca
    23341 tgagtccata tgagaatcga acaccgaatc tttctaagga gagataccag gttacaggag
    23401 tagtttcgaa tccaatggct caattagaag cttcaaagtt tacgatcaat agtatggcta
    23461 attacttatg gaactgtgag cgttttgatc ctttggaaac ttggacgtca gtcgttgaaa
    23521 aagtagttgg aagtaaacta caaccagctt atcttacctt aaccaatgct tttccaaatc
    23581 attacacaag cagtctgctt tcagatgaag aattactgct agccaaagac ccagaatggg
    23641 tcacaaaaga aatggctgct ttagtccaag cagtaaaatg gatccagcaa gaacaacata
    23701 catctagatt gagaacagaa ttggagcctt ggttacaagc aatcacagaa agttggactt
    23761 tctggcaaga atggcaatta aaaaaagatc caaaagaagc agaagaatgg ttggctaaga
    23821 aaaaaacaca ccgcatagga cgtgatttag taactgccca tttagagcaa attatcaaaa
    23881 gttagatatc ttaaaaaata aaaagcaaga aaaaggagaa caaaatggtc aagagattaa
    23941 ttagtgcaaa ctacagtgat gtcaagaaaa tgacgtcaaa agagttgaag caatccatca
    24001 aagcgagtga aggacgggtg attgtttgtg aaacggttgc taatactctt ccacaatctg
    24061 gagaaattac gaatgcagaa gttgcgtcag catttggtgc agatttgatt cttttaaatg
    24121 gtttcgattg ttatcgtcct gcagtttttg gactaccaaa tgtgacgatt gagaaggttt
    24181 ttgatctaca gcaaacgatt gaaaacccga ttcctaaatt aaaagaacta gtcggtcgtc
    24241 ctgtgggaat taatttagaa cccattgccc ctaaaacaaa tatgatgagt gagaaagtag
    24301 aaatcagcca aggaagagca agctctgttg aaacgataca aaaagcagaa gaactaggag
    24361 tcgattttat ttgctttacc ggtaatccag gcactggagt aaccaattta gaaattacta
    24421 aatccgtaaa aatggcaaaa aagtattttt cgggattgat tattgctggg aaaatgcata
    24481 gcgccggctc agaagagcca gttgtgaata aagaggcgat tatttcttat gcggaagcag
    24541 gcgcagatat tttactagtt ccttcagtag ggtctatccc aggcttttta gagcaagacc
    24601 taattgaagc ggttcggttg gcaaaaaaat atgatttgtt aacgatgtct gcaatcggta
    24661 ctagtcagga aagtgcccga ccagaaacat tgcggcaatt agccattatg aataaaagtt
    24721 gtggtgttga tttacaacat attggagatg ctggatacgg gggattggca ccagcagaaa
    24781 atattttcga aacctcggtt gcaattagag gaatgcgaca taccatcaac tgcgttagcc
    24841 gctcgatcaa tcgctaacaa aattaaataa aaaatggtta aattgattat ttaagtggga
    24901 acatacttga gatatcagct aacttctgtc actttaaaaa agggactgtg acagaagtga
    24961 aaatagacaa aaaatatccg aacaagagac taggatagca gaaaaactat cgttttgcaa
    25021 cgaggaccaa ctggaccgta ggagcaattc tcttattgtt cggatatttc gcttgttcaa
    25081 ggacttatgt ctgtagtgcc cttttttggt aggaaaaatg gtagggaacc atatcatttt
    25141 taatgaaaat caagcatttc aaaagt
//
LOCUS       DAZULG010000028        25123 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000028 DAZULG010000000
VERSION     DAZULG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.028"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACG9CY_001057"
     CDS             6..245
                     /locus_tag="ACG9CY_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937582.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACG9CY_001058"
     CDS             312..521
                     /locus_tag="ACG9CY_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937583.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACG9CY_001059"
     CDS             581..1252
                     /locus_tag="ACG9CY_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4937584.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVLAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACG9CY_001060"
     CDS             1305..3281
                     /locus_tag="ACG9CY_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4937585.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDITQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3296..4048
                     /locus_tag="ACG9CY_001061"
     CDS             3296..4048
                     /locus_tag="ACG9CY_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4937586.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4064..4819
                     /locus_tag="ACG9CY_001062"
     CDS             4064..4819
                     /locus_tag="ACG9CY_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4937587.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            4832..5092
                     /locus_tag="ACG9CY_001063"
     CDS             4832..5092
                     /locus_tag="ACG9CY_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937588.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5089..7179
                     /locus_tag="ACG9CY_001064"
     CDS             5089..7179
                     /locus_tag="ACG9CY_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFS4937589.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7206..7670
                     /locus_tag="ACG9CY_001065"
     CDS             7206..7670
                     /locus_tag="ACG9CY_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937590.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            7667..7909
                     /locus_tag="ACG9CY_001066"
     CDS             7667..7909
                     /locus_tag="ACG9CY_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFS4937591.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            7927..8229
                     /locus_tag="ACG9CY_001067"
     CDS             7927..8229
                     /locus_tag="ACG9CY_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937592.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8299..10362
                     /locus_tag="ACG9CY_001068"
     CDS             8299..10362
                     /locus_tag="ACG9CY_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937593.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10362..12974
                     /locus_tag="ACG9CY_001069"
     CDS             10362..12974
                     /locus_tag="ACG9CY_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS4937594.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            12971..15502
                     /locus_tag="ACG9CY_001070"
     CDS             12971..15502
                     /locus_tag="ACG9CY_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS4937595.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15552..15884
                     /locus_tag="ACG9CY_001071"
     CDS             15552..15884
                     /locus_tag="ACG9CY_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937596.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            15885..16478
                     /locus_tag="ACG9CY_001072"
     CDS             15885..16478
                     /locus_tag="ACG9CY_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937597.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            16641..18665
                     /locus_tag="ACG9CY_001073"
     CDS             16641..18665
                     /locus_tag="ACG9CY_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFS4937598.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            18665..19864
                     /locus_tag="ACG9CY_001074"
     CDS             18665..19864
                     /locus_tag="ACG9CY_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4937599.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            19880..20524
                     /locus_tag="ACG9CY_001075"
     CDS             19880..20524
                     /locus_tag="ACG9CY_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937600.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            20535..21341
                     /locus_tag="ACG9CY_001076"
     CDS             20535..21341
                     /locus_tag="ACG9CY_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937601.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21363..21593
                     /locus_tag="ACG9CY_001077"
     CDS             21363..21593
                     /locus_tag="ACG9CY_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937602.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            22107..22415
                     /locus_tag="ACG9CY_001078"
     CDS             22107..22415
                     /locus_tag="ACG9CY_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937603.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            22417..22839
                     /locus_tag="ACG9CY_001079"
     CDS             22417..22839
                     /locus_tag="ACG9CY_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937604.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            22859..24448
                     /gene="mobP2"
                     /locus_tag="ACG9CY_001080"
     CDS             22859..24448
                     /gene="mobP2"
                     /locus_tag="ACG9CY_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFS4937605.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            24460..24795
                     /locus_tag="ACG9CY_001081"
     CDS             24460..24795
                     /locus_tag="ACG9CY_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937606.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            24936..>25123
                     /locus_tag="ACG9CY_001082"
     CDS             24936..>25123
                     /locus_tag="ACG9CY_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937607.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         9386 a         3904 c         5155 g         6678 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtacttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcagaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgagtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ctatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttattggaa tgttaggaat cattgcaggc tgctttcagg
     1381 gatatcgagc atatgcagaa gatatcacgc aaaaaacgcc accagaaaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgactgaag
     1621 attctgatct aaccaacgcg aaagtggacg aaatcgttaa agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacaa acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagattac ttttgctgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gactgataat acaggttcag gttttttaaa agacattcac ttatatccta
     1921 aaaatattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcacagctc
     1981 aaagcaaaca aacaccttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attaggagat cttgtatcgg atgatcaagg aaagctgact gctactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcaga agttgtagtg ggatcagata
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacaattca tatcccaaca gacattgcag
     2401 gcggagcaga cggtatcaca gtaaacggag tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg ggatacagca ggacaaggac tttcttatgt cgctgctaaa gcaaatatca
     2521 aagtaacaaa taaagatgga tctgtggtat taaaagaaaa tacagattat aaaatccaaa
     2581 attcagaaaa tggctttgtg atcgatttta tcgtgaataa tggccaagta agtgatactg
     2641 tagcaagttt acacggacaa gatcttcaaa tgacttataa catgtatgta aatgacagcg
     2701 cagctgttgc caatccatta acaaatagcg tggatttcgt ttataacaac aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggt gcaaaattct
     2821 taaaagtcga tagcggacta tttggaacag gaatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgctgc taaaaacgca gaaggaaaat attatggtgg attagttgat acggataaag
     2941 acggtgtcaa agaagcagta tgggtagacg atgtagcaaa tgcagcgatc ctaaaatcag
     3001 ataaagaagg tcactttgaa atcactggtt taactgaagg tgaatattca ctggaagaaa
     3061 caaaagcgcc tgaaaactat caaaaattaa cgaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaacaa tcaaaaagct tccgtaccaa
     3181 tgaccggtag caacggtttt caaacgtatg tattaatctc ttgcttgttg ctaggagcag
     3241 gggcactttc tgcagttgtt tactttaaga agaaagccta gaaggatcaa aaccaatgat
     3301 tatcaggaag cctaagaaaa agcgaatcat gggtaaatgg atcattgctt tctggttact
     3361 ttcagctgta ggtgtcttac ttttgatgcc tgctgaagcg agcgtggcga aataccaaca
     3421 aaatcagcag attgccgcaa tcgatcgaac aggaacggca gcagaaacgg attcttcttt
     3481 ggatgttgct aagattgagt taggcgatcc tgttggtatt cttacaattc taagtatttc
     3541 tttaaaacta ccaatttatg acggaacgtc cgacaaaatt ttagaaaatg gtgtaggtat
     3601 tacggaagga actggtgata ttacaggagg aaacggtaaa aatcctttaa tcgcaggtca
     3661 tagtggactt tataaagata atctttttga tgatttacct tcagtaaaaa agggagaaaa
     3721 gttttatatc aaagttgatg gtgagcagca cgcttatcaa atcgatcgga ttgaggaagt
     3781 gcaaaaagac gaactacaaa gaaactttgt cacttactta gaatcaaatc ccaatgaaga
     3841 tcgagtaacg ctaatgacat gtacgcctaa ggggatcaac acacaccgtt ttctagtgta
     3901 tgggaagcga gtaaccttta caaaatcaga attaaaagat gaagaaaata agaaacaaaa
     3961 attatcttgg aaatggcttt taggaagtac tgtttttctt tcagtcatga tcattggttc
     4021 gctatttgtt tataaaaaga aaaaataaag gagggtaacc aacatgagaa atagaaaatg
     4081 gctatttgtt tgtgcagccg ctcttttttc tttcactgcc tacacacagt tgacgtttgc
     4141 agacagacaa gaaagcacgg atagcacaga acaaacaatc caaacgtcag atagcagcac
     4201 gattcaaagc agcgatgaaa ccgtcgattc gtcaacagtt gattcttcag aagaacctaa
     4261 aattgctgaa gacgatcaaa tagtcaaaca gaccattcac gttcaaaaga ttatttcttc
     4321 agaacaatta gacaaccagg ggcatgaaaa acaagaagga gtaaatggcg cgaagttttc
     4381 tgtttatgat gtatcggaca ttttacaaaa gatggatgtt aaggatctca ctactgacca
     4441 aattgaatcc caattaaaaa atcgagtgaa aaaactatcc agtgatcaat tgaagctagt
     4501 ttctaatgaa gaaacaaaga caattgatca acaaacaggt gtctttgaat tctcggtaga
     4561 agtacaggca aatcagaaac aagcttatta tattgtcaat gaatcaagtc ctgaaaacat
     4621 ttctaatagc gaggatattt tgctattaac acctgtttct gataaaaacg gtgaattctt
     4681 aaaggatgtg tggatctatc ctaaatcaga agcttcgcaa cctaaagaag aagtaaaaaa
     4741 aatcgtttct actggcgtaa agaaaaactt ctttgagaat tgttgggatt ttgttactca
     4801 cttgttttct agagattaag gaagaaataa aatgaaactg atcaaattat tattattcaa
     4861 tttttcttgt ttaaccgcag tctttttgta ctgcggttta ttattttcta aactatctat
     4921 tttgccaagc taccaactat acctaatgag gattttaaac ataccagctg cttccatgcc
     4981 gctagatatt atggaaaagc tcattaggaa tccattattt actacgatca atttgatcgt
     5041 tattgtcgga atttcactag tcatttataa aaaagagggg atgaagggat gaattttaaa
     5101 cactcgtgga caagacggtt aaaatatgtt tttcccataa tgatgatgtt aggtacattg
     5161 tttgggttaa aaacagccaa tgtctttgct gaaaaagtta ttgtcgatcc gtcaaatcca
     5221 atccagaacg taaagaataa ttttattatt ccaaaagatg ttcctaatgc aaaaacaaat
     5281 attactgtaa acggagcgtc aaaagtttac aaatatcatg ttgatgccaa caaaggtggt
     5341 tttacagacg actacgtagg attagtggag ggaaacaaaa atatacgcta tccttctttc
     5401 caaaaagaat acggtgaaac aaaccttgtt ttagaaggcg taacaaccaa caccaaagtc
     5461 aatattgact atggaaaaat cggtacgtat aacgggaaaa aagtcaatat caagctagtt
     5521 ctatctaata ttcacttata ctcggatacg ttgccgtgga atctactaga taacaactat
     5581 acaaaaactc attttcgaga cgatggctat aaaaatacga atggtgcaat gagtaaatct
     5641 aaaaaaagaa cagtactttg gatctcagac aatcttttct caggaattgt ttaccattca
     5701 acacaaatga atgtgcagct agtagccaca tatgaggacg gtagccccgt tcaattttca
     5761 ggagatacat ttatcagttt taactctttg aaccctgcag gaggaaaaag cacggatctt
     5821 aaaggagaat atgcccacta tgacaaaatg aatacgacag attggtatgt cagaaaagat
     5881 acggttcttt cagaatttaa atctttctac aacaatctga acgtggtagg tggccaccca
     5941 ggaggaagct ctaagctgac acaagcggat aatgatttca ataatttgca tgataaatta
     6001 ggagatccga agtttggtca aggtactgtg tcctttaaaa tatctgaagc aaatccgacg
     6061 tttgtgattg gttcttctaa tgttcaaacc tggtttactt tatcttctgc gacaatcttt
     6121 agtgttgttc ctgatcaacc tgaaaaaaca ggggttgata aaaatggaaa taacgtgaac
     6181 gataaaatgt tgcaagtagg agacacgatt caatatcgaa tcaaacagaa ggtgaatcga
     6241 ttaggagtgg atttactagc ggtttacgat cgatttgagt taatcgataa tttaccaaaa
     6301 gaagtaaatt acgtcgatgc acacgttgaa agtggtacaa ataaaaaatt cgatgtatct
     6361 ggtgaagtga cctatgacaa aacaaaacac caggtaaaat atgcagcgaa agctgataca
     6421 ttaaaaaatc gaatgaagta taacggcgaa acttacgaat tagtgatcaa tgtaaaagtc
     6481 aatgaattgg ccaatcaaaa tagtgtagcc aaaaatcagg gaacgtcgat cattaataaa
     6541 gtagaaaagg atacaaatat cattaccgtt tatttcccta aaattccagt gaaagaagta
     6601 caacaaaatg gaaaagatgt aaacggacga aatgacggta agaagggtac gccgacagct
     6661 cctttaaacg caggatctga agtacaatat ctagtcacac aaaaatggca cacaaaaggc
     6721 gtagacgctg cgtcagatca ctacaaacaa tttagtattc aagatccaat tgaagctcgt
     6781 ttgacatata aagaaggttc tgcgcaagta atagataaga gtaaaggaaa agatattact
     6841 tctgaaggca cactaaccta tgatagcaat tcaagaacgc tgaagtggga agcttccgct
     6901 gacttcttaa gtaacaatct tttagatgga cgagaaattc agctgatatt tacagctaaa
     6961 actccattac aatcagaaaa aaatattgat aaccaagccg tagtagcagt agataatgtt
     7021 tctaataaaa caaatgttgt gacaattggg gttgatccta acttgccaca ggtcattgtt
     7081 cctaaaacag gttctacaca tttagtaacg atttcagcgg tcagcttact attacttgtg
     7141 ttagcaactt ttagttatgc ggttctgaga tacatttaaa ataaaaaaac gaaagaatga
     7201 ataaaatgaa aaagttaata atagtagtta cttccttgat tgccatgatt gttattttag
     7261 tcggatgtag taatgaaaaa aataaacaaa ctaatcaaga taatggcgta ttgaaatcag
     7321 gaactatgtg gaaagaagaa gtaggtggtc ttgtttataa tttgaaaatt atagacgaga
     7381 ctacctggga atactctgag agtgtatggc atccagatcc tgtacaaata acagtcaaga
     7441 ggcaaaaaga ctataaaggt ttagaaagat acaaaatagt agattcagct ggtgttaggg
     7501 aatttataaa caaatctgat tctttattta ttgttgttcc ctatgagaag aatggcgtaa
     7561 aaaaaataat ttttcttgga tctagcaagg atgaaaagcg aacgaaagag aaattgattc
     7621 acgatggatc acaatccaat aaatataagt tacaaaagac tagcgagtga ttgctagtct
     7681 ttttatttgt cctgaaggag gaatcacagt ggcaaaagct aaaacgccat ttgaaaaagc
     7741 cgcgtctaaa atcttagaac gccgcggtgt atctgaaaca cagtggaaac aagaagtcct
     7801 tgaaaaagca caatttgacc tattacgggg aaaagacaag gacttagaag gatatatcta
     7861 tcaacgtgaa cgtgaaaagc ttgttttcga tgaaattatt aaacactaaa aggagcgaac
     7921 aattttatgt ttgaatcaat tacaatgaat tttttaacac aaccgaaagt agtagctgcg
     7981 gttgatattt ttagagcatt acaaactgcc gcaactagtt taaaaaacaa tctgatgatt
     8041 tttgctgctg ctgcggccgt tgcttccatt gttattgggg gtcttttctt cctatttgga
     8101 cgaagaggag ctgaaaatgg gaagatgcac gtaggctatg tattgatggg ggtaggagtt
     8161 attttagcag cagcttctat tgtggtttcg ttatttggat tgtttggaca aacacctcaa
     8221 ggattataag aaagtaaaaa agtaggagta agaagtagtc gatacttttt actccttttt
     8281 tatgaaagga gaatactcat gtactttacg attgcaggca ataccaaaac agataaagag
     8341 ttaataaaaa aagcaacgaa ggaagtagga aagcgtactt cttttttacc gaaaacaatt
     8401 aaatattctg tcattgaaac ggaagaacag ctacatgtgt tagggaaagt actggaacac
     8461 gaaagtcgat cttctgttaa gttgactctc acgttacatg acacagaagg aaaaatagag
     8521 aaagctggta cagaatacgt ttttggccca ctggattatc aaatttcaga aggtttctat
     8581 cctacattga tggatttaat ccgccttgat gtgtttaaag aaacggatga aactatgagt
     8641 gtggaagagt cttatcgaaa cgaaaaactt ttacaagttt tagaagaaga gttagagagc
     8701 gaccaaagaa aagtgaatca agggagtgaa gtcgaagtaa atgaagaagt accatgggaa
     8761 cgaccaatat ttccaaagaa ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa
     8821 gctgatattc cgactcagaa atcgatattt gaggaagaga acgatgtgat gggtcaattg
     8881 gaggtagatc cctttgaaga aacaatggag gatgaagaga cggccgaagt agaagttgat
     8941 ccagttgaag cgaatgaaga gtcaacatgg actgaagggg attctcagga atatattgaa
     9001 ccacttatca gtgaagaagt agaaaaaaac gaaatagaga tacaaaaggc aactgtattt
     9061 gattatattt tggatcaata tccgccagag catgaatgga tgaaagaaaa aaacaaatta
     9121 tttttaaatg agctttattc gaaatatcaa ttaccccaaa cctatcaagc atttttagag
     9181 agtaaagaag aattaatctc acagggtaaa ggcacacttt tagaacagtt agagtctgtc
     9241 caatcaacaa attggcatga gcttgcacaa gaagatttat tagccacctt tgaagaacgt
     9301 caacaagaaa cagatcaaga aattaatgag tatgcgacgg aacagtcgtc taattgggag
     9361 aaagcaaaag aggaactaaa taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa
     9421 attaaagagc gatacgaaaa gaaacgcgac aatgcgtatt ccttgtgtgt agcaaagatc
     9481 aataatttta ctcagacgaa tcaagaagcg ttatcgaaag aaaaagaaaa cttgttaaaa
     9541 gaacgtatta aagaacgaaa agaagaattt taccgaggtt tgcgtacaga taagctaaag
     9601 attagcaaag aattaaatga acaactgaat aagctatatc aaacaacgac tgaagttctt
     9661 atggaaaaat acgaaaagat ccagcaagaa ttacaagtgc aaattccaaa atggaaaaaa
     9721 gagtatgaaa ttcaacaaca agaattgaaa gaacaagaag aaaaacaacg gcaaaaagaa
     9781 aaagagcgcg aacaattaga actaaaaaaa cgtgaacagg atcttgcgga acaacaaatg
     9841 gcaatagaaa aacaacggtt aaaccaagaa aaggaaaaag aagcagctgc tcgtaaggaa
     9901 cgtcaagaac aaatggatac attgcgacaa gctcagttaa attatctagc acaacctcaa
     9961 ccaacgcctt cagtagctgc tgttcaaacc ccaatgaaca accaaacaaa tctaactcct
    10021 tcaccacagc gttcctttaa cggatggatg gtgggatgta ttgcaagttt aagcttgctt
    10081 ctaggtggag gaacagtatt tgcttttaat catatcaatc aatcttcaca ggctgattct
    10141 gtggccacaa cgacgtcaat caaggaagaa gcccaagaac aagcaagaaa agagtatgaa
    10201 caacaattag aagaattatt gaatcgtacc aatcagtcaa caacagagtc aagcatggaa
    10261 aatagcaaag agagtgattc tagtaaggct aaagaaggat caagcgattc aactgtacaa
    10321 tcaaacactt ccgaaagttc agaatcaaat gaggtgaaat aatgcgtgtt agagaaacaa
    10381 cgaagcgaaa agacaaacga ttcaatttag aaaagaatag gtatctttct catttctatc
    10441 gctatactcc ctcaggagaa gagctaccag aaaaacatga caaacgcatt tcgaaaaaga
    10501 ttttttatag tggacttgta tacctccctt tagagtattt agtaacaaga gtaccagaag
    10561 ttttcaaccg attggaaatt gttaagggaa gcttacaagg aataaatctg tttcaattac
    10621 aggaaatgcc acaggtcttt cattttgatt cagctggttt ttggaatcca tttactccta
    10681 caactagttt gggtacacgt ttagcaattt cgacagtttg tgtggttcca gctgtactta
    10741 tgggattgaa aatggacatg tggtttcgtc ctctagcaga tggacaaaaa ggggataatc
    10801 gattaatgac cttaaaagaa atccaacaga tttatccgca aatacctgaa acaaaaacta
    10861 cttttccagg ttatggaggt attccaatca cacattacaa aaaatattgg tacattcaaa
    10921 cagatactgt taatacgtgt attgttggta cctctcgatc agggaaaggt cagattgaag
    10981 tgttagccac cattgacaat ttatctcgtg cagaaaaaca aagttcaatg gtcgttaatg
    11041 atccaaaaac agaattatat gttggtagta aagatgaact agaagctaga gggtatgatg
    11101 tatatgcctt taatatcacc gatccactgc aatcaatgag tgataatcca ttggcactca
    11161 tcgtgaaata ctggaaacga ggagacatag atactgcaac acagttaacc aatactttca
    11221 caaatacgat ttattatgat gccaacgctt cagataacaa atactttaac gaaaacgcgc
    11281 agaaagctgt caatgcgctt atttttgcgt tgttagaaca agctgatcaa acaggagatt
    11341 actccaaatt gacaccgaat aatctcgttg agcttctttc tgagattggc ggttttaatt
    11401 attatccaga tccagacaat gaattcaaac agttgaatgc cttagatgaa tttatgaatc
    11461 agttgccacc tggaaacatt gcaaaaaaac aatatggggc aacgaaaatt ggatctgata
    11521 aagcgaaggg aaatatttta tcaacggcaa tcacgggatt gaatccattt actctaacga
    11581 aaattgcaaa gatgacttct caatcaacga ttgattataa atcggttggg tttccaaaat
    11641 acttagattt gaaattacat gaaagtcttt taaatcaacg agtcgtagta gaattttatc
    11701 ataagggaaa gaaatttcat gaagaacgtg tcaaagtagg ctataaagga ttttgtgaaa
    11761 tcaattttga ttgtcattta gttgcaggtg atcaagtttg tttaaaatac gatcatcaag
    11821 gaaaagaata tgtggcttgg tatgaatttt ctcaaagagt attgaaagac gaagagggta
    11881 atatcgttta tcggaaaaaa cgaggagaaa cccatttgcc agaattagaa aaagaagccg
    11941 ttctaacatt agatgaggag aaaagtaact tattagtaga gtctattcgg atgcactata
    12001 ccgataaacc aatggctatt tttatgctta cacctgatta tgatccatct aaccatatca
    12061 ttgttagtat ttttcttagt cagttatata aagaattatc tacgcaatgt gttaagacta
    12121 aaggagataa atgtcaccgc aggattcatt ttattttaga tgaatttggg aatatgccac
    12181 caatggataa tatggaagga atcatgacgg tcacagctgg ccgaaacatg ttgtgggatc
    12241 tgtttattca atcttatcag caactttatg cgaagtatgg agaacaaaac gcagcgatta
    12301 tcaaagaaaa ttgtcagaca catatttata ttatgtcgac agatgataat accatcgaag
    12361 aattttctaa aaaagtgggg aataagacgg ttgaacaaga gaacagtacc atcaattcat
    12421 taggtatgaa tactcatatc aatcgcaatg ttgattcaga tcgtatctta actcctgaaa
    12481 gaatttctac ttttttagaa ggtgaaacga ttgtactcgc tcctttaaaa cgacaagatt
    12541 tgaaaggtca taaaatacgg ccgtatccta tttttaatca tggggaaacg aatcttcctt
    12601 atgcttatca atatattgaa gatttcaatc catcgtggga tatcaatgat atagacatac
    12661 attgtacaca tgcggacttg gatcttaagg ctttacaaat tgattatgat tatttcctac
    12721 cagatgaata tgctagaaat ctctatcatg gactgcatga tcaattagaa gaaaaaaacg
    12781 aagaaaatat gttttataaa atcgttcaat ctcttgagaa tgagacggct aaaaaattgt
    12841 taattcaatt ttacgaagaa caagcagcag atcgacttca agaatatgtt gaaaagttga
    12901 tcaaaaataa agaaatttct gaaatgattg ggaaacggtt gttattggaa tctgaaaagg
    12961 tggtgaaaga atgatcattg gaggaatttt ttctggcata gccgactggt ggtcaggctt
    13021 attcggagaa ggggatgcaa accccaaaac cgtaacgaaa gtcttacaag attatggtaa
    13081 ttatctgcag tatcaagatg tatttagttg gatttggaac acttttctgt ggggattgat
    13141 taaaggatta tataatctca atactttact agaaaaaacc atttatcaaa gctttgattt
    13201 aaaagactta ttgaacgccg ctggggtaaa tgcactattt caaagtttga ttagtaaggt
    13261 attggctctt ttttgtgtcg tcacattaat ttatttaggc ttgaaatttt ttatgagtaa
    13321 acacccacca aaagtcaaaa atatcttggt taatttattt atggccatga tcttgatttt
    13381 tggtgggagt tctttaatcg atgaagggtt aaatatcagt aaaagctttt acggagacgt
    13441 cactgcagca aataaagcag ataaaacggg ttcacctgct tttcaaatta ttcaaaataa
    13501 tgtgatcgat gtcagtacat tactagcaac tgacccgaat aaagtagaat ccctgccaac
    13561 agacaaaaga aatgctttaa cagcgaaaaa ttttaaaaca gcgaatatca atgggattat
    13621 tactcctaaa caagctaaag aaatggcgga taaagataag agtggtttat cagatttaca
    13681 aaaggatcgg ttaagagcgt tacaataccg attggaacta gatgataatg ggaaagaaat
    13741 tcctgttcaa attagtgacg aaggcttagc aaaatatgtc tatacttcag ggtatcgtcg
    13801 atacagtagt aaaccaggta ttatcttagc tggtgaatct tctttggcgg tggcctattt
    13861 atttatccta tttacgatcg ttacttgcat tattgagctg gtatttaaaa aattctattt
    13921 agtgattgcc gcgtcaacgg actttgagac aggccaacgg atgaaaagtg cgatagaaga
    13981 tatttctcaa tccttcttgt tattggcgtt tacgatttta gaattacgaa tctatacatt
    14041 aatgctttca ggtatcggtg atttgcatgc ggcaggaaag atcaatggat tcttgtatgt
    14101 tgttgcttta attgtgctaa ctattgcgct attcaaaggc tctcaatcgg ttacgaagat
    14161 tttcggtgtt gatacgagtc tgaaaaacgg cagtaattct cttcttagca tgtttgcctt
    14221 aggaaatatt gcgaaaaatg tgggtagcgg agctaaaaac cttgcaagtg ctggtaagag
    14281 tggtttaagc catttaaatg atattcgtaa aaatggattt ggccatcgaa aagatgttac
    14341 ccctgataac ggggaagaac aatcagacga taaagctaca actgtaccta atagtaagaa
    14401 aacagtttcc gaaatgttcg ggaaagcgaa aaatggtgtt tctaaagccg cagaaggtct
    14461 tggttacgtc aatgaacgtg ggttaaaagg aacagctgaa gatcttacag aaaaagccaa
    14521 agacacctta gaagagaaat tagacggcac gagagctaaa gaattggctg aagctgtgaa
    14581 gaatccatcc gaagcactag aaaaacaaaa agaaaaagct tcagaagtca aagaaggact
    14641 aaaagatgcg tatggtgaag gacaagttaa tgcagcagta aaagctaata caacaaccga
    14701 aaaagaaaat caagaaggta tagtgattcc ttcagtcaaa gatcaccaag aagcgttgca
    14761 ctctaatgaa aattcgattg aagagaaaaa ttccatagaa gaaagaaatt cagaagaagg
    14821 aaaaaatgga cagttaccaa aaactgatcc agaacaatat cgctataaac ctaacagtga
    14881 ccttgaaaat gagaagcgaa tttcagattt atcaaaagta aatcaagaag acaaagcaga
    14941 aaatgaggga cagttaccaa aaactgatcc agaacaatat cggtatagat ctgttaactc
    15001 cgaaaatgaa aaacgcattt cagatttacc aaaacgaaat caagaagtac aaacggatga
    15061 ggattccatc gttctgccaa atgataatgg aatcgaagtt ggaactgaaa aatcaacgaa
    15121 aattggttta cctaaggcac aaaatttacc aatgaaagat agtcgtttga atcaattcaa
    15181 ggagaatgat caaagctttg ttcaccaatc aactgcaggt actgttcaaa tgaaaccaag
    15241 tcgtttgcct gaaaatggaa atctgacaac tgtttcttac aatttatctg aaagtggtga
    15301 gacagggaat gcgtccgttc aaaatgttgt gacaagagaa gctgcaccaa aacctattca
    15361 aaacgttcat acaggttaca agccagcaaa tgtggcattt actgcaccaa caaaaataaa
    15421 tgtgacaaga gaaactaaaa acaggaacaa tgcattgaaa gaacaactat tgagggatat
    15481 caaacaggat atcaaaccat aaaaggccta caagttaggc cttttattta tatgaaaagg
    15541 agaaatcaat tatggataaa tatataaaca tgaaaaaccc tccaagattt agtggagaat
    15601 ggaagttttt cgcttttttc tttttagatc tattaacgct tggtggctgc tttttttggg
    15661 cgaaaatggt caataatctt gtccacttac cactcttttt tgcactcttg aattatggct
    15721 tatggattgc gatagcgtta gtgattgttt ggcgacccta taataaccca ggtcaacgac
    15781 aaatttttgc gattatcgag tctatttttt ttgcggacaa gaaccattat catgcgttag
    15841 atcctaaccg aaattatcat ggtacgtata aggaggatga ataaatgcga gtcaaaggac
    15901 aaaattcagt tgaaaggaaa tccattttgc agtttggaaa accagaagag cgctctatct
    15961 tggacttatt tccgtataaa tcctgtacag aagagggagt cttaattact agagaagatc
    16021 attttcaacg ttattatcgt gtagtttcta cagacgtgga aggattaaat gaacaagaga
    16081 aactagaacg aatgaatcag ttgaccaccg tcatgcgtac gtatgtgcca acaattaagt
    16141 tagttagttt gaccacagag acggatctaa cggaacaaat cgtgcaaaag cggcaattat
    16201 tagataaaaa tcgtatggaa cagtccttag gaagaaatct acaacgatta aaaaaatatg
    16261 aaaagaaatt agtcgaagaa atccaggaat tgaaacgagc agaacaagaa cgtcctgact
    16321 taagcttctt tttcgtcatt gaaggaaaga caatcaagga tttacaaacc agaaaccgtc
    16381 agttaatgcg atctagtggc attttagggt taaaaccact ctacaaaaaa gaattgatca
    16441 agattctgta tcgaatgaac aatatgaatg atgagtagga atggagaaga gagaatgaaa
    16501 tatccgttga tatggttaga aaaagatgtt ccgaaggaag agaccactag aaaatttcct
    16561 cacactttaa gcctgggtaa gttgggaaaa gggcaatctt ttaaccaagt aaagaatcaa
    16621 acaagaagag gtgactgtac atgttagaag caattaaaac agctttttca ccacaaaatg
    16681 atcaaaaagt acttgatcca ttaaagcaac taggaaatcg ggaaatagaa attttaaaat
    16741 ccaaagggta tgatttagat tttttaaaac aagtgatgcc acaaggtggg ttaaattttg
    16801 atgaagaata tgtgacgtat ggtaatggtt attcacgatg tgtgacagtt tatcactaca
    16861 caaaaaatcc tacgcttttt tggttggcca atctcatgaa caatcctcat acagtggcca
    16921 cactcgatgt tagcacagat gaaaagcaaa aagtgattga tcaaatcaat cgtgccttag
    16981 atgaattaaa tgatcgatca gataatgaac gaaaagcgac tgaccgaaat aaaagcgcag
    17041 aagaatacct ggaactagcg gaatatgctc gtaaattgac ttctaatggg gaaatttcta
    17101 agcaagtcaa agtgagagta catgtttacg gaaacacgat tcaagaagta gatgaacgag
    17161 cgcaggaact gatcgaaaat ctaaaaggga tggattatcg tgcggtgact tatctgcata
    17221 aaaccaaacg agattggcaa tcgatgttta ccatgttcga tgaacaagct gaatggttcg
    17281 gaaatatcaa agggcaatca ctaccatcgt taaacattgg cggaggtttc ccgttccacc
    17341 accaaacgct aaaagatcca ggagggacac aatatgggga aaccatgaca ggcggcccat
    17401 ttgtctggaa tgcgacgtat aaaaatagtg tacggttgtc tactaatacc ttgcttctag
    17461 gaatgatggg agctggtaaa tcgaccgttc ttaaaatgat cgcggaagcc catttagctg
    17521 caggagactt tatttggggc tttgaaaagg gaaaagactt tattcctttc ttaaaggaat
    17581 ataacggtat tatggtgcgt ttagacggat ctgacggaat gatcaatcct ttagagatat
    17641 ttgccacacg gacgtatgat gaagcatcca gtttatatga cgatggatca gtcaaagatt
    17701 tgaagatcaa tgaagcagcg tcctatcaaa cacacttaga caaagtggtc tatcaggtac
    17761 agttagtgag tcctcagcta aaaggaacga tgaaagcaga atttaagacc tacttaaatc
    17821 gtttttatga agagtatgga acagtaccac gcggctttac ttcctcaaat tcgcgttcaa
    17881 atacggagac gcaagtcaca ggtaaagatc cagaagctta tccgacgttc aaagaatttt
    17941 tagacttttt aggacaactg gaattaccag gtgcttccca agagaaaaag aatcgaaaag
    18001 aagaaatgga atccatcgta gaatccttat gtgagacgta tggcatgatt tttgatgggc
    18061 atagtacgat tcgacacctt gaccaacaac agttggtctt ttttgacatt gactctatta
    18121 caggcttaga tcgaggggta caacaatgtc aaatgtacat ggctatgacg ttgatttgga
    18181 atcaggcatt gattcaagga agaagacaac gacgtttaat taaagaaaaa caaatggaag
    18241 aacaagactt caaacgcttc ttggcgatga ttgatgagtg ccataatgcg attaatccag
    18301 attttattga aacggtaaat tatgtcacaa actttcaacg agaaatgcgt aaagtagaag
    18361 caatgactgt tttagctaca caatccccac aagaaatggt gccagaaaat atgtcttctg
    18421 ataacttctc gaaactgaag aaagtctttg aattttcttc tacgaagatc tttatgcgta
    18481 tggatgaatc tattttacgt catatagaag gattagtggg taaatcaatg acgaactcag
    18541 aattagaatc cattcctcaa caaaatcaag gagacgctgt gatcaatttt ggctcaaaag
    18601 aaaccattcg tgttcatttt agtccgaaca aacgtcaatt aaaattgttt gatggaggaa
    18661 aataatggaa aaccaaaatg aaagtttaat caaacagtat gttaagcgtc gtgcaaagcg
    18721 gcgcttattt ttgtgggtgt ttggtacttc tgcaggtttg attactatat tgatcactgt
    18781 gtttgtgacc ctttttctaa tacttgcggc aggttctatt gataattctg attcaagtag
    18841 tggtggcgaa gcttttacag gagaatattc ggaaggctta cccatttata aagaaatcaa
    18901 aggaagaggc cctttttctg atgagattgc gcaatatgct gtaggtgcag cagtgaagta
    18961 taaattatta ccaagtgtga tcctttcgca atacggttat gaatcagcgt ttggtacgtc
    19021 ggcgtccgca agaaatgatc tcaattattt tgggattacg tggtttgatg ggtgtctgtt
    19081 tcctaaagga actgcgcgtg gaattggtgg cattgaaggt ggctggtaca tgaagtttcc
    19141 caactcaaaa gctgccttct cctattacgg ctttatggta gccacacaat caaattttaa
    19201 tgcttgtgtc ggaaataaaa gccctggcgc aagcttattg atcttaggac gaggagggta
    19261 tgccgccgca ggaattactg aagacagtgc ctattataaa aattgtatgt ctattattaa
    19321 tacaaataaa ttgatggagt acgatgagtt tgcgattaaa cactggagag aaggcggcgg
    19381 aactggtggt acgattacag gaagctgggg caatccattc ccaggaagta gcttagataa
    19441 aaattcgttc agtggaggac aactttttgg gaaaaatcca ggaggagaat tccgacctaa
    19501 cgggttccat gatggactag actttggttc agtcgatcat ccaggaagtg agattcatgc
    19561 ggttcatggc ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt tgggtgcgtg
    19621 tgtgatcgtg attaattacg atggcttgaa tatggtgtat caagaatttg caaatagtac
    19681 aggaaattct cgagtgaaag taggcgatca agtgaaagtt ggtcaagtaa ttgggatacg
    19741 agatacagca cacttgcatt taggatttac tcgaatggat tggcgtcaag cacaaggaca
    19801 tgcattcatt gatgatggta catggatcga tccgttacca tttttgaata gcagcaaaaa
    19861 atagatcagg agaaaaaata tggaaaataa aaccttaaaa atcgtcttaa cagctgcaat
    19921 tagcgttagt ttaatcggca atattgcgtt gttttggcag cttaaagaag aacaagagac
    19981 aaatacctat aaaattgaag aagtggctaa aaacgcgtct gataagctca aaaaaaattc
    20041 ttctgaggca caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga aaaaccaaga
    20101 aatcgatcag ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa ctgaagcaca
    20161 aagaaagacg gcagaagagt tcggtcaatt ggccattgat aaatcattaa gcagaaatga
    20221 attaacggaa aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat taagtcctgc
    20281 agacgatgat caccaacatg acgactacgg aagttactct tttacattag gtgacgtgca
    20341 aacttacgct caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat ttgttgatta
    20401 tacaatcggc aatcctcaat ttaaagatgt caaaccacaa aaaataaaag gtggattaac
    20461 tgtcactgaa aaacaagtag atggtcaatg gaaagtcaca gactttagtt atttcactcg
    20521 ttaggaggaa gaaaatgagt aagaaaaaag gagcaagaca acgttttaaa aaacgaaaaa
    20581 gttatcttag atggctgggg atctttatga ttctcggctt tttgattctt gctagtccga
    20641 tttacacaca taaaaaggta gacataccag atgaagtaag cacgaacgca tttttaaaca
    20701 cgaaggaact gacgatggtt tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat
    20761 ttgcgatcga cgatgaagat cagagcttat taaaagaact agctaatctt aattatcgtg
    20821 tagaagtaaa gacagctaaa ggggattatc aagcaataaa agccaaagtg atcaaggtgt
    20881 ctgatgatta ttttgtagtg aagttagcac atgtaccaga agaatatatc gccatgcgtt
    20941 taaccattat tcctgaaaaa attgatccaa aagtggatat gcaagaacca caagatctga
    21001 tttattacgt tcacgaagat aaggttaaag atcaagtgaa agatgaagat tatgaacaac
    21061 atgcgatcaa ttacaaagtg aaaggataca aaaaagaaca aaaagcagca caaaaacaaa
    21121 tggaatcctt ccaagcaacc attgatttga atgaagaatt agtgaagaag ctaaaagatc
    21181 aattgcctta tcaagtcgca gatgatcagg aaaatactga aaacaagatt aatagctacc
    21241 agcaagaaat tgaaacatcg aaaacgcaaa tgaaagatca agaagagatc attcaaaaac
    21301 ttcaagaaaa aattgatcgt atgacaaaag aaaagattta gaaggacagg tggagtgaaa
    21361 aaatgaagcg aacaaacttg gctgacaaag atactttcgc tgagcagaaa gtattgatta
    21421 aagcctatct caaagttgta aaagctaaag aaatatgtcg agaagtgaat ttatctaata
    21481 caacaatatc tgagttgaag aatggtcgaa agaatttaga tactttaaaa ttcagatcat
    21541 ttgaagctct atatgtagct gctaaaaaat attttacagc tgataacaaa taaaaaacag
    21601 gagtatttta tgtgaaaaaa gtatataaaa aatgcttatg aatcagctat atcaccttaa
    21661 ccatttgtta atgagatggg agaaaacatt aaccacgatt ctaacgcaaa catcaagcac
    21721 ataaaacatt taacactcct cctttctggc tgaagatttc aaccaaacag gaagacagcg
    21781 tatcaattat agcatacact ttttcttaaa ttcatttttc atatgacaaa atatttcctt
    21841 ctaatcgaca aagttagaag gattttttat tgtctaaata cacaaataac cttaatttat
    21901 aaagagcata aggaaacgtg ggaacacttt ttaaaaaaag tgttccaata ggctgtgcct
    21961 attacgtttc tcaaaaataa ttgtgggaac aaaatattta aaaataggat agtgaaagga
    22021 tagcaaagga tagtaaaagg atagaagcag gatagcgaaa aggataggct ccttttcgct
    22081 atccttttta aattggagga attaatatgg aagtaagaat aagaaatgta gatgagaaaa
    22141 tcgtaagaga aattgatcga agatgtgacg aactaagtaa ccgaacgggt aataaatgga
    22201 gtcgtaacga ttatttaaag ctactcgttg aaaatgattt tgatcgtcct ttgatggaat
    22261 ataaacaaga taaatttgat caattattaa atcagttaat agataaattt tcttttgttc
    22321 aaagtcataa tacaaaagta ttagaagaat atatttgtca gaataatcaa attatcgaac
    22381 ttttaattga acaaagtaga ggaggagaag tttaacatgg ccaaagttat agacaagcat
    22441 atttattttt atgaaacaca tatagatgtt ctagatgaaa ttattgcaac tcatgcagag
    22501 gttaaaaact atagtgaagc cgttcggttt ctatgtatga attatgattc agaagtggag
    22561 aaaaaagtaa attccaatca agcaaagttg aatgcaatgg gaaaagaaat ttctatttta
    22621 accgaaatgg tttcagagtt tgcggatctc catttgaaag atacagggat tttaattggt
    22681 acaaattcaa ctgtttacca agaagcaaaa gagcgtgtgg aaggcaagat aaaagcaaat
    22741 caaacgaaga aatattctcc taagataaag aaaacaataa gcgaatcagt gactacaaaa
    22801 agtgaagtat ttgatccaac aaggttctta aaacgataaa aattagaagg agaatgtgat
    22861 gagtaaatca cctgcagtca ttctcacttg tcaatttacc ttgcccaata aaaaatcttt
    22921 ttcaacatac attgattata tgacaagaga aaaagcgctg gaagaaaagg aacgacgtac
    22981 gcccgaggaa gaacaggaac tgcgtctaat caagaatgct ttagaggatt tttatattcc
    23041 tgaaggagaa actttttcgg agagtaagac gggtaaagaa ttcagcgaaa cgtcgaatga
    23101 agcgcaatcg ttgatgaaac atggaatcaa ttttgacaag ttagaagaac aagattttac
    23161 caaatatctt tcttatatga cacgtcacta tgcgttagaa caaaagaaag tactgacgca
    23221 tagtgaacaa aaagaatacc aacgcttgaa ccaagcaata aaaaaatatc aaacagagcc
    23281 acacaaaaaa gcttcaggaa atcttcctgg agttttttct atggcttcag acgaagtgaa
    23341 aggaagtgat ctgaaggaga tcaagcaaac cttccaaaga ggacaacaaa aaggctcaat
    23401 catttatcaa gatgttgtct ctcatgatca ttcgtatctt gaaaaaatag gattgtacga
    23461 tcccaaaaca gatactttag atgaagaagg tttaaaagca gctggaagaa aaatgatgga
    23521 gacattgttc gagaaagagc aactgaatga aacaggctac tggatggcaa cgattcaccg
    23581 aaataccaaa catatccata ttcattttgc agttgttgaa aaaaaaaata cgcgtaaacc
    23641 atacactgag attgaaaatg gtgtgagtta tatcgtaccg aaagggaaga gaaggcaagc
    23701 aacattagac gaaatgaaga ctaattatgt acatgagtta gaacgttttt cttatgaaaa
    23761 aaatcgaatg ggtatggaga aacgaaattt attgacacgg aaaagtgatt tgcgtaacac
    23821 cttaaccaaa gtagtgaaag agccagtacg ttatgatcga cacgcaatga gcttattaac
    23881 tgaagtatat aaaaacttac ctaaaaagcg ttcggaatgg cattacgggg atgaaaatcg
    23941 aaccaaactt tcaccataca caagagagaa actcgatgag ttaacccaat atgtgttaac
    24001 aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagcaaa
    24061 aagtctttat ggggaaagca agagagaatc aaaggatgca gaaaaaaatg ctctctttga
    24121 tttaaaaaag cgcacaggaa atgccattct aacagaactg aagaaacagg atagtcaaat
    24181 aaaaccttta atagaaagga tggatcagat gcaatctatt tacaatgaac gatcctttcg
    24241 aacacttaaa cagttgccaa cgaatgaata caaatatagt tacgaagaat tcatgaagcg
    24301 caggaaacaa ttccaacaaa gagttgtcac gcaacggagt ttgcgtcaat taaatcgaac
    24361 gattaatgcc catgaagaaa aataccacgc aagtaaagca tacgaagagc aacaacgaag
    24421 aattcaaatg gaacaagaaa gacagtagga ggaatagcaa tgaaacaata tttagatcaa
    24481 tggaaagtta tagaaggatc attaagagaa gaacgaatcg aacaacttcc cgattgttta
    24541 gaaaaagagc atttgtttca gataagagaa atgttgataa acgaacagtt tgatccaaat
    24601 caatttttag tcgttgagta cccagctact ggtgtgtact gttgtaatca tgtgaagggt
    24661 gaaaaatatt ttatcattca agaatatgag ggcaagttag cgccttatta tactacttgg
    24721 gaaatgaatg aagaggggat caataacttc ccgtgtaaaa gcattgaaga gtctatttca
    24781 ctcacagaat gttagttgtt aagtgaataa aagaagtaaa agagtcctga acgtgattgt
    24841 ccaggactct tttttttcac tgaattgttt tccgccaatt cattgagtgg tgcgatttcc
    24901 gtctacatta tatcaaaaga aaggaggaag aagaaatggc ttattcaacg attggagttg
    24961 ttgttgaaaa aagcagagac aatcttgttt ttgtgacaga aattcaaaca ggcagagctt
    25021 ttgtagtcac agacaaagcg gcaaaagcat atcaaagtgg cgatatttta acgttaaatc
    25081 tgactacgaa gacgttcgtc gatgcggcag aagattatcc att
//
LOCUS       DAZULG010000029        24355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000029 DAZULG010000000
VERSION     DAZULG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.029"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..881)
                     /locus_tag="ACG9CY_001083"
     CDS             complement(33..881)
                     /locus_tag="ACG9CY_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4937608.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1105..3045)
                     /gene="thrS"
                     /locus_tag="ACG9CY_001084"
     CDS             complement(1105..3045)
                     /gene="thrS"
                     /locus_tag="ACG9CY_001084"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS4937609.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3382..3561)
                     /locus_tag="ACG9CY_001085"
     CDS             complement(3382..3561)
                     /locus_tag="ACG9CY_001085"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS4937610.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3608..5431)
                     /locus_tag="ACG9CY_001086"
     CDS             complement(3608..5431)
                     /locus_tag="ACG9CY_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4937611.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5709..7988
                     /gene="helD"
                     /locus_tag="ACG9CY_001087"
     CDS             5709..7988
                     /gene="helD"
                     /locus_tag="ACG9CY_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS4937612.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8081..8728)
                     /locus_tag="ACG9CY_001088"
     CDS             complement(8081..8728)
                     /locus_tag="ACG9CY_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937613.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8907..9626)
                     /locus_tag="ACG9CY_001089"
     CDS             complement(8907..9626)
                     /locus_tag="ACG9CY_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4937614.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9757..10908)
                     /locus_tag="ACG9CY_001090"
     CDS             complement(9757..10908)
                     /locus_tag="ACG9CY_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS4937615.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11125..11865)
                     /locus_tag="ACG9CY_001091"
     CDS             complement(11125..11865)
                     /locus_tag="ACG9CY_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4937616.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11869..12192)
                     /gene="rsfS"
                     /locus_tag="ACG9CY_001092"
     CDS             complement(11869..12192)
                     /gene="rsfS"
                     /locus_tag="ACG9CY_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS4937617.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12249..12842)
                     /gene="yqeK"
                     /locus_tag="ACG9CY_001093"
     CDS             complement(12249..12842)
                     /gene="yqeK"
                     /locus_tag="ACG9CY_001093"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS4937618.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12826..13476)
                     /locus_tag="ACG9CY_001094"
     CDS             complement(12826..13476)
                     /locus_tag="ACG9CY_001094"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS4937619.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13486..13797)
                     /gene="yhbY"
                     /locus_tag="ACG9CY_001095"
     CDS             complement(13486..13797)
                     /gene="yhbY"
                     /locus_tag="ACG9CY_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS4937620.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13813..14922)
                     /gene="yqeH"
                     /locus_tag="ACG9CY_001096"
     CDS             complement(13813..14922)
                     /gene="yqeH"
                     /locus_tag="ACG9CY_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS4937621.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14919..15446)
                     /locus_tag="ACG9CY_001097"
     CDS             complement(14919..15446)
                     /locus_tag="ACG9CY_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS4937622.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15626..16411)
                     /locus_tag="ACG9CY_001098"
     CDS             complement(15626..16411)
                     /locus_tag="ACG9CY_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS4937623.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16404..17273)
                     /gene="accD"
                     /locus_tag="ACG9CY_001099"
     CDS             complement(16404..17273)
                     /gene="accD"
                     /locus_tag="ACG9CY_001099"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS4937624.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17274..18653)
                     /locus_tag="ACG9CY_001100"
     CDS             complement(17274..18653)
                     /locus_tag="ACG9CY_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS4937625.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18661..19080)
                     /gene="fabZ"
                     /locus_tag="ACG9CY_001101"
     CDS             complement(18661..19080)
                     /gene="fabZ"
                     /locus_tag="ACG9CY_001101"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4937626.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19105..19581)
                     /gene="accB"
                     /locus_tag="ACG9CY_001102"
     CDS             complement(19105..19581)
                     /gene="accB"
                     /locus_tag="ACG9CY_001102"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS4937627.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19584..20819)
                     /gene="fabF"
                     /locus_tag="ACG9CY_001103"
     CDS             complement(19584..20819)
                     /gene="fabF"
                     /locus_tag="ACG9CY_001103"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS4937628.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20838..21575)
                     /gene="fabG"
                     /locus_tag="ACG9CY_001104"
     CDS             complement(20838..21575)
                     /gene="fabG"
                     /locus_tag="ACG9CY_001104"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS4937629.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21587..22504)
                     /gene="fabD"
                     /locus_tag="ACG9CY_001105"
     CDS             complement(21587..22504)
                     /gene="fabD"
                     /locus_tag="ACG9CY_001105"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4937630.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22611..22835)
                     /locus_tag="ACG9CY_001106"
     CDS             complement(22611..22835)
                     /locus_tag="ACG9CY_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4937631.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22888..23850)
                     /locus_tag="ACG9CY_001107"
     CDS             complement(22888..23850)
                     /locus_tag="ACG9CY_001107"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS4937632.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23860..24300)
                     /locus_tag="ACG9CY_001108"
     CDS             complement(23860..24300)
                     /locus_tag="ACG9CY_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4937633.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6664 a         5425 c         4245 g         8021 t
ORIGIN      
        1 ctaaaaaact tatataaaaa atagtaaggt ttttatttcg aatccataaa atattcttgg
       61 attgcttctc gtgttttttt cactttttcg ctaagctttt cgtttttagc catatagaaa
      121 taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg aattagttgc cgcacttcga
      181 ttcgtgattt cttttggtct agatgtttgt acgagtacat cggctatctt gctaagcggg
      241 ttatttccaa attgcgtcaa cgcaatgatt tcgatattca tgcttttcgc cagctcggct
      301 aatgcgacta catctgcact tctacctgag tttgaaacaa gccaaagcac tttctttttc
      361 tcattgctga caagttgcgg gagcaagacg tggatatctt tttcaaaaat gattggctta
      421 ccgacacgag accatttttg cagaatatct tctactacta aagaagaggc gccaacccca
      481 tataaaaaaa cagtctctgc cgattccaaa cactctacga ctttttggat cgtttcttct
      541 tctagaatat ctactgtttc ttttatcgct acttgtgcat tgttaccaag tttgtttttg
      601 atagagtaaa aggactcatt tgcttctata tcactaaaac cttcatcgat tccagtctgt
      661 agttctgttg aaatcgtgat tttgaagtcc gtcagacttt gaaaaccgat ttttttggaa
      721 aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg ctaattcatt agctgtcatt
      781 gtagggacct tctcaatatt accgataatg tagttggcca actttttttc tgctgaagaa
      841 taatcttcca tcatactttc aatacgacta attacgctca taagttcctc caccctttta
      901 atgatagtct atgtaagtat accatatcag acccttattc acggtcgtat actggataaa
      961 aaacaaaaaa acaatccgcc caacagaata tcttatccca tcaatcccac agattctatt
     1021 gagcatctgt gtctttgagg aatacctgtt atctattgtt cggattgctt tctatctact
     1081 acttttttac aggaaattta cctattagtt ttctctgctg taatttttga cttctgcttc
     1141 catagcatca ataaagatat tcaaatcttc tacagctgtt tctttacttc cataacgacg
     1201 tacgttcact gttgcatctt ccaattcctt gtctccaaca actaattggt aagggatctt
     1261 ttgtgtttga gaggcacgta tcttatagcc cattttttca ttacgatcat caacttctac
     1321 acgcaacccc ttcatttgca aacgttcttt gatttcataa gcataatcgc catgtgcatc
     1381 aacagaaaca gggatgattg ttgcttgtat aggtgccaac catgttggaa aagcgccttt
     1441 gtatacttct gttaaataag cgacaaaacg ttccattgta gaaacaattc cgcggtggat
     1501 aactactgga cgatgcgtat tttccccgtc ttctccaaca tatgtcaaat caaagcgctc
     1561 tggcaataag aagtctaatt gtatagttga taatgtttct tctgtaccaa gagctgtttt
     1621 gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg ccttctgctt caaagtaatc
     1681 tagttctaat tcatccatag cggctttcag cattgcttgg gctttttccc acattgcgtc
     1741 atcatcaaaa tatttatctg tattgtttgg atcgcgatag ctcaagcgga aacgataatc
     1801 tgtaatattg aagtcagcat atactgccac catcaattca agcgtacgtt taaattcatc
     1861 tttgatttga tcaggacgaa caaatgtatg accatcattc aacgtcattt ctcgtacacg
     1921 ttgtaaacct gacaaagcac ctgatttttc atagcgatgc atcattccta attcagcgat
     1981 acgaattggt aattcacgat aagaatggat cgtgtttttg tagaccatca tgtgatgagg
     2041 gcagttcatt gggcgaagaa caagcatttc gccatctccc atatccattg gcgggaacat
     2101 gtcttcttga taatgatccc agtgtccaga tgttttgtac aactcaacat ctcccattat
     2161 tggtgtatat acatgttggt aacctagact aacttctttg tctacgatat aacgttcgat
     2221 cgtgcgtcgg attgttgccc cttttggaag ccagaatggc aaaccagaac ctacttctgg
     2281 ggaaaccatg aacaagtcaa gttcttttcc tagtttacga tggtctcttt cttttgcttc
     2341 ttcacggaga cggatatatt ctttcaaagc tttcttgtca aaaaaggctg tcccgtatac
     2401 acgttgcatc atttgattgt ttgagtttcc acgccaataa gcacctgcca ctgataataa
     2461 tttaaatact tgtatacggc cagtggatgg aacatgtggt ccacgacaca aatcgatgaa
     2521 ttcgccttgt tgataagcag taatcacttc ttcttctggc aattcactaa tcagttctac
     2581 tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt tttgaaagaa cacggcgttc
     2641 gatcggcaga ttttctttga cgattttcat catttctgct tcgattgccg gcaaatcttc
     2701 tgcagttact tggttttgtc cattatctgt atcgtagtaa aaccctgaat cgatagctgg
     2761 tccaacgcca aagtggatat ttggatagag gcgacgcatg gcttgggcca tcaaatgagc
     2821 tgctgaatga cggactaagc caagtgcatc ttcatggtct ggtgtgatga tctcaatact
     2881 tgcatcttcc tcgattggac gagtcaaatc aacgagtttt ccgttcactt taccagctaa
     2941 agcttttttt gctaagcttt ttgaaatgct ttcagcaatt gccaatgttg ttgttccaga
     3001 ctcaaattct ttgactgcac catctggaaa agtaattttt atcataatag tttcctcctt
     3061 gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa aaaaatccta gcatctcttg
     3121 aaaagagata ctaggacgaa aagttcgtgg ttccacctaa tgttcaaacg caaatgcgtt
     3181 cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca agggtttcga aagtggtcat
     3241 cctttggttt tctcctggaa aagtttcagc ctagcttttc ctctcttatc gaagactctc
     3301 aaattcagtt ggctttctca tcaatcaatt atgtgtttat ttaaccacta ataaatagaa
     3361 aaatcaagtg ggaaactaaa cttatttttt ccattcccca tttacaccgt atgttccttt
     3421 tttcatctca ttgatgatga catggatgtg ttcttttggt gcctgcgtgt ttttatgaac
     3481 agcttccgtg atatctttca tcatattttc taattgttcc ggactgcgac cttctactaa
     3541 ttcgacatgt acgaatggca taatgctctc tcctctttct gatcgttctt ttttcaactc
     3601 gattctatca tactatgttt gtcttgggta ctgaatcagg aaaaatggct ggatcttttt
     3661 ttgctctcaa ggcactcaaa atagagaccg tatccacgac ttcttgcaaa acagctccga
     3721 ataatgcggg gattagtccc gtacttgcaa ttaacatcaa aaaaatacaa ataaagatgc
     3781 caatcagtac agactgtttc gctattctca tcgtatctct tgaaatcact actgcttttg
     3841 ctacacgcaa gagatcatct ttcaagatga ccgcatctgc actttcacta gcagctgtag
     3901 aaccatgagc ccccattgca ataccgatat ctgcagcagc aagtgctggt gcgtcattca
     3961 cgccgtctcc taccatcgca acaggtcttt gatctttagg gatactttct aaaatggtca
     4021 gtttatcttg aggcaagcta cgggcatgta cagaatctaa ttggactttt tctgcaatcg
     4081 tttgagcaat ggcagatcca tccccggtga tcatcatcaa atttttcaca tcaagttttc
     4141 ttagctcctt gattgtcttt gctgcttctg ggcgaatctg atccgaaaat aggatcgttc
     4201 ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt ggacgtttct atttcttcag
     4261 ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt attgactact gcttggattc
     4321 cttgaccggt cacttctttc agttgactaa caggatacag ttggctgttt ttcacgttgt
     4381 tcactaacga acgagctaag atatggctag attcctgttc tgcactagct gcatacagaa
     4441 gtagctcttc tttttttatt ccattgactg gctgaatcgc ctctacttcc aatatccctt
     4501 tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt acgtgcgagc ttttcaatcg
     4561 ttgttccagt ttttacaacg atcccgtttt tacttgaacg gctcattcct gcaactaaag
     4621 caatgggcgc agctagaatc agtggacaag gtgatgccac taccagtacc tcagcaaatc
     4681 gtaaagggtc ttttgagaac caccaagaaa taccaccgat caaataagca atcaacgtga
     4741 aagggactgc ataacgatca gctaaacgga caaatcgggc aggcttttct tttgattctt
     4801 cgactagttt aataatcatt tgatactggc tgtctgcggc acgtttttca gcgatcattt
     4861 ggatcgatcc gtctccattt aaagaccctg acataatctg atctccaggt ttcttggttt
     4921 ccgggcggga ctcacctgtc aaagaagatt catcgaccga agattctcct ttggtcactc
     4981 gaccatctgc gggtacaagt tcgccaggct tgactagtaa ctggtcatca atatttattt
     5041 catcgattac tatatcctgc aattttcctt ctttgaatcg atgggcaatt cttggggtgt
     5101 tatctaacaa agactgcagt tctttacccg ctcgtttatt cgcgtaatcc tctagactat
     5161 ctccgcccgt aagcatgatc aatatcatca aactagccca gtattctttg acggctaatg
     5221 ttgcaaggat tgcggtaatc gctaatacat ctacgccata tcttcctgaa cgtaacgtct
     5281 tgatcatttc caaaaacatc aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa
     5341 atgcaagggc cggctggtga aaaacaaatt cacttaataa agctatccca ccaataagca
     5401 aagtaagtaa aaattttttg aaatgtgtca ttgttttttc tcctctttta tgtgacttag
     5461 agaggttagt aataaatttc cagctgcctc tttcatctta agcatagtcc ttgactagca
     5521 ctttttaaaa caataggcct taattgaaaa caactatcac tatcagcgtt tccaaaattc
     5581 atcagttcta caaacaaaaa tattcagcaa ggattacttt ttcgattcat cagttaaaaa
     5641 gtaccttttt tcgaaaaact atgctatact tttaattcgt tatttacggc ggaaggagca
     5701 aacatgcgat gaatgagaaa caactagaac aaacgcacgt ggatgaaacg atcgaactaa
     5761 tccagatcga acaaaatatg ctggatacaa aacaacaaga attgactcag gaaatgaatc
     5821 attcgacaaa agacacagct aatcacaaga ttcgagctgg ttccaatgaa gctttttatg
     5881 aatctgctgt tgaatatcgc caacatgagc aggaattgct tttgaaatac catactcttg
     5941 aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa agatagtcct tattttgccc
     6001 gtatcgactt taaagaagac cgtgaaacag aaacattata tctaggtatc gcttctttaa
     6061 gagatctggc agaagaaaca atcgttattg actggcgcgc accaattgcc aatctttatt
     6121 atgaaggtga gattggtccc gcattttatg aaactgaaac agatagaata gatgtcgatc
     6181 tgcagttgaa acgacaattt aaaatccaag aaggacgtat tttatctatg gtagatacgt
     6241 cggaagtaat caatgatgac tttttgctag agatattgga tgaatcatca agcaaccata
     6301 tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc aatcattcgg gacacaacaa
     6361 gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac
     6421 gtattgcttt tctgttgtat cataatcgca agtggctcga ggctgaaaac gttcttctat
     6481 tttcacccaa ccatttgttt tctgattaca tctcgactgt attgccttca ttaggtgaaa
     6541 gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca gctgctgcca gagttttctt
     6601 tactagatga acagcaacaa gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag
     6661 tgatgaaaag cggattgacc ttagtcgatc agattgatcg ctatatccaa ggcatcacaa
     6721 actacggtcc cttgttccgt gatatgaaaa taaatggccg tacgatccta tcaaaggaat
     6781 cgattcgcca atggtataaa gaaacaaatg aacaattacc tttacatcat agactttctc
     6841 tattacaaac gaagctcctg aaaaaattag gcggtctgca aaaagacgaa acacgtcagc
     6901 aatgggtcaa agatctggcg gaagaacagc tccaagaact atacgcaaca gatccaaatc
     6961 ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa actggccaac caaatcgtac
     7021 gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta tcaatttgtc aatataacga
     7081 aacaatatct ccactttcta caaactgtgc cactttctct tctaacacga cataaaatca
     7141 gcgagaccag gtggcaggaa catttgacaa ctgttcgtac acacttgcgt caaagagaat
     7201 tgcgtcaaga ggatgctgta ctattctttt tactgatgcg ccgaacatat ccagtgacgg
     7261 ttacacacaa agctcgctat atctttattg atgaaatgca ggatttcgca ccatcacaag
     7321 tggcattgct gcaagccttg ttcccaaaag caaatttgac tttctgtgga gatctaaatc
     7381 aaaatgtctt tggaaatgaa acgatcgttg gatcattgga agaccttttc gtggaccgtg
     7441 aagtcactcg tttccaatta acgacaagct atcgctccac aaaagaaatc actgattttg
     7501 ctaatcattt tattgtgaaa gaacatcaag tagaaacaac agcaagaaaa ggagaccttc
     7561 ctttgctcat ccaaggaaca acagatgata tcaaattgaa ttggttagca gataccattc
     7621 gaacgactca gggaaaagcc cggtattggc gtacagcaat catagctaaa acacaagaag
     7681 aatgcgaagc tctttatgct caactgccag acgagttgaa acaacaagtc cagctgattt
     7741 taaatgagag tgactttatg aagcgttcca ttattttact tccagccttt ttagcaaaag
     7801 gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc agaacatttt tcttctcagc
     7861 aagacaaatt gatcctttac accatgtgta cacgtgcgat gcatgaattg acccttttta
     7921 cctccaataa tgaaagtcct ttgttaaaca atatggatat ttcatcttat gagaaaaaag
     7981 tactgtaagt aaaaagacaa ctgctatcat taatgttagc agttgtcttt ttatttacaa
     8041 taataattgc agaaaaagta attcaatcat ttaaggtcat ttattctata tataaatcca
     8101 aatctgtttc aggatatttt aatttgacaa gcgaaataat cattttcagt tcttccatgt
     8161 tttctggata ttctgggtca aataccttgc tatgacggcc aatcccgcca ttgtcgatca
     8221 atcggtaaat tcgattccat tcaatgaatt ggtcaattga ttggaaattg ctgattggat
     8281 agccgccaca atccaaaatc tctgatacac gaaatgctag gtatgcacta tcgatcactt
     8341 tatttttttt cgtgtctatc ttctcatttc tttcgataag aattttttca ggacgatgtt
     8401 tcaagctcca ctctaatagt agaaagaaag cctccatccg atactcttct tcaaataacc
     8461 gaaaggctga atcagaacga cgaaaaagca gcggaatcag ataattttgt ctataaatca
     8521 gcattggtgc gcggtccacc atacactcta aataacggtt cagataccct gtaccgacat
     8581 agtttcctaa tcgatagcct tcaacgccga atcttgcgta tttttgcgct aattttgaac
     8641 aatcagttac gttcacagct aactcttcta ctttgcggta accgcttaag atacgttcaa
     8701 aaggttctaa agtgattttt gcttccatat tcttccccgt ccatcttcca catacttcat
     8761 atcatttcac atgatactta tgctctcatc ataccacagg ttgaattaga aaagaaaata
     8821 aatcacacaa aaaagcaaga caatctgtta gtgagattgt cttgcttttc gaaaaagact
     8881 gaatactttt cgttaaaact ggttgcttag tcttctaatg ccacattatg gaacacatgc
     8941 tgtacatctt ctagatcttc cagtgcatca atcatttttt caaatgtttc ttgatcttct
     9001 ggagatagtg ttacctcgtt ttgagggatc atttggatct cagcgattga aaaatcagtg
     9061 attccttttt cttttaatgc ttcttgtata ctatggaaat ccgctacttc accgtaaacg
     9121 atgatttgtc cttcttcttc cattacatcg cgaacatcga tgtctttttc cattaggtat
     9181 tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg
     9241 taagaaaccg ctccggaaac acccatgttc ccgccatttt tcccaaatgc tgcccgtacg
     9301 tctgctgctg ttcgattcac attgttcgtc aatgtatcaa caatcaccat tgagccgctt
     9361 ggtccaaatc cttcatagcg caattcagag tatgtttcat ctcctgcacc ttttgctttt
     9421 tcgattgctc ggtcaataat atgttttggt acattataag ttttagcgcg atcaatgacg
     9481 aaacgtagtt tttggtttgc atgcggatca ggatcacctg attttgctgc cgcgtagatt
     9541 tcaatcccaa attttgcata aatccggcta ttattagcat ctttagcagt tttctttgct
     9601 acgatatttg cccatttacg tcccatattt tcacttcgct ttctgatttg atagtttcat
     9661 aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg attctgtgaa gctttttttc
     9721 tttcttcctt cattcttctc atttttagtc aattatctat actcggattg ggatttgccc
     9781 gaaattctgt tccggaatat tgcctcccaa ttgatagatt tgatcacttc taactaacag
     9841 ttccgcttct tgtgcctctt tttttccgat tcgggaataa atagaaatct cttttttttc
     9901 ttttaagaat cttcttccag catcggtaaa tccaagtagt cgaagataat ttttattttg
     9961 ttggacagga atctcttgct ttttgatatt cagcaagaca taacaagata atcgttggat
    10021 ccgtgtccat gtatatcttt ttgtttttac ctttgacaca aaatcctcaa aactatcagc
    10081 agttgcagct gctgttttca tgcgcgattc tattccttcg gtcatctgat agatatctgt
    10141 cagttccagc aaagtagaag ataacaaccg ataacgcaaa tacggccaat aagattccca
    10201 agtctgcagc gaacaagttg aaagatcttc ttctgtctga ggcggcacaa gtccagctat
    10261 tctttttcct tcttctaacg catggcgaat ggctgttgca ctggctatcc tctgatctgt
    10321 gatgattttt tcatgatagc ctgcactttt acgtcggatc ggatgtaatt ccatagggtg
    10381 gacatacttg gcattttcct ttgcataact taatcccaag atatgatttg gcgaagaaaa
    10441 atcaagcgta atatcgggat acagactgcg aaaaacctca gtcatttttt gcggataact
    10501 atacgtttga tcagacagtt cttgaaatgc ctcttctatc ctatcttgat tctcttggac
    10561 aaagcgtccg aaactgctgt aatcaaacga agtctcttct tctgttccaa aacacaaact
    10621 gtcacagtcc agtgtttgca agatttttac tgcaccttta gcaaaatagt cagctgactg
    10681 aaccgcccat tcgacgggaa gctccactac taaatccaca ccattttgta aagcttcatt
    10741 cgcgcgatgc catttatcga tgatagccgg ttcgcctctt tgcaaaaaat tcccgctcat
    10801 tacagcaatg acgacctctg ctcctgttat ttttcgtgcc atttccacat gatagcgatg
    10861 tccattgtga aaaggattgt actctacgat aatcccgcag cttttcatgc gcatcctcct
    10921 tcatagttat cgataaaaaa gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa
    10981 tacccgaact atataaacta ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact
    11041 gcaagagcaa tgcctatatc gttcggatat ataatttatg ttgaagattt attcttttct
    11101 tgttaactcg agcaggcaaa accgctagtc tttgtatgct acaaagaacc agcgctttga
    11161 tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct
    11221 gttttccagc attcttaaat aactttccaa tggataggta cgctcttgat gtagttcatc
    11281 ttcacgaata aatcgaccag acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc
    11341 gatgctgtgt ttctcgcttc ctggataact atcccacaaa aaagcaaaat tatccccttg
    11401 ataatgatag ctatattctg gaaaaacctc atctacttga taggtggagt gtacgtcaaa
    11461 taaaaatcgg ccattttctt ctaacgcttg atagacttca tcaaacactt gctgtacctc
    11521 ttgtctattt ttcatatagc acagcgaatc agaaaaacag gtaattgcct gatattgtcc
    11581 gatttctgat aaatcgagca tattgccttc cacaaattgg atcgatgcgt cttcttcata
    11641 tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg tctaaccctg tcactttata
    11701 tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg catgccagtt ccaaaatatt
    11761 ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac cattgctgat aaagtgtttc
    11821 gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa gccatatgtt aatccaacca
    11881 ttcactaaga tcgactagcg gagcatctga ccatagtttt tccagattat agaaatttcg
    11941 ttctggcgca tggaaaacat gtacgatcac atctccaaga tcgatcaata cccattttcc
    12001 gccttctttt ccttcaatac gttttacttc atatttgttt tcttcttctt tatcaatgat
    12061 ttcttctgta attgcgttga tttgacgttc gctatttgct gaacagatca tgaaataatc
    12121 tgccaacaag gaaacatttc gaacgtctaa agcgataatg tcttctgccc gttttgaatc
    12181 tgctgcttca atcgcaattt ttaattgttc tttactatct atgatgattt cctcctaata
    12241 tgagcagctt atttttttgc tacccagtaa ttatatgttt cgattgtttt tggataaatc
    12301 tgttgttctt gcgcaagcaa atgggccaat gtatgttttg tttcaaatgc tacagcttcg
    12361 tctagatcag cccatgcaat ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt
    12421 tcaatatagt ctgctacata aatgattttg tcaaggagac tcatcttagc agccccagtc
    12481 gtatgcaccc ggattgcatg aagaatctct gcatcggtga ttcctaattc ccgctctaca
    12541 aacgaagcgc caacaagacc atgccagatt gcattattat aattcaataa ctccggatca
    12601 aaaccgtctc ttacgattac cattttgaac tcctcatctg gtcgttcttt tgcataatca
    12661 tgtgtcaatg cagcaatact agctttttca ggagaagcac cgtatttttt ggcaagagcc
    12721 acggctgttt cttctactcc aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt
    12781 attttttgca tcaggttttc tctagaataa gcagtatatt cattactata gtccatattc
    12841 atacaaccct tcattttgaa tataatcaat tactttatct ggaacaaggt agcggataga
    12901 acagccttct ttgatctttt gacgtatttt cgtggaactg atgtcgatct caggtacatc
    12961 tacccaaatt acaggatatg gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa
    13021 gttgaccata gaagtcagct catcgatttt ataccatttt ggcaaatatt ctaccatatc
    13081 cccaccgata ataaagtaat aatctgtatc cggattgttt tgcgtcaatt ccttcatagt
    13141 atcataggtg taacttttcc caccacgagc taattcaatc gtttcaatct gcaaaaaagg
    13201 attgtcttcc accgctaatt ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt
    13261 ctcatcaaca tgaggtggct gataagaagg catcaaaaat acgcggtcaa gacctaaatt
    13321 ccggccagct tgttcagcca tcactaaatg tgcaaggtga acgggattga atgttccgcc
    13381 caagatccct acttgctttt tcttctctaa gaattgaggc atttcttcag gaaataattg
    13441 tgaacgaaca aatgtcttag cttgcgtatt catcatggtc actccttata ttgcttttac
    13501 ttcttttgat agcctttgat acttttcttt tgtggaaggt ttgtataata caataacacg
    13561 tccaatgatt tggacgattt cgcaatctat ttctttttct aatacttgag ctgcttcttc
    13621 tgcaatctca tctgtatttt gaagtaacga tactttgatc agttctcttt tttctaaagc
    13681 ttcttcaatt tgaacgatca ttgctgaatt gatcccacct tttccgattt gaaaaatcgg
    13741 ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt tttcctctta aactcataat
    13801 tttccttctt ccttaaatca atgctttgcg tttcaataca tctacaccct ttggcgccca
    13861 gcccgcaatt actactggcg ctgttacagt gatccagcct agaccggcaa aaacgatgtc
    13921 tgttttttct ttcacagaaa actcaaaacg aacaagctcc ggaaatgcct cgacttcatc
    13981 aggacgagga ggctgtaata atccacctac atgtttttga taaaattcgt ctgctttttc
    14041 cagtttcgtt cgatgaatat ttacatcatt tgacacataa gcaataaatg aagcacgatt
    14101 gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag
    14161 ctgataaact ttaggtttga tttcttttaa cggtgcaatg atccgcaaat cttttttacc
    14221 taagtagtgc gccatttgat ggcgatggat aattcccggt gtatcaatca aaaaatgccc
    14281 gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat
    14341 tacatcttgt actcccgctg tatttttgat gatttgattg ataagcgtcg attttccgac
    14401 atttgttact ccgacgatat agacatcccg tccatcgcgg tattcttcga ttttttctaa
    14461 tagttccgtc atctcatgag atttcttagc actagttaat aatacatcta ctggacgaat
    14521 tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat ttagtttttt tcaatgattt
    14581 aggtaatata tccactttgt tcccaactaa aagtactgga ttgtcaccga caaaacggtg
    14641 tagtcctggg attagtgaac cattgaagtc aaaaatatcc accacattga cgatcaatgc
    14701 atctttttgc cctaattcat tcagtaagcg caaaaagtcg tcatccgtca atgagacatc
    14761 ttgggtctca ttataatggc gtaatctgaa acaacgctga caatagactt ccccggaagc
    14821 taaacctttt tccaaagccg tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg
    14881 tataacggca ccacagccga tacattgtaa ttcttctgtc attcgattcc acctcgccag
    14941 cccatatcag ggtgttttgc taatagatag gccataattc ttttttccca aaaacgatta
    15001 aattgtgtct tccaactatc tgttgcgata atgggtttta caaggatgct acgtatccct
    15061 gcacgattgg ctccacgtat atcagtcata atctgatcgc ctaccattac tatctcatca
    15121 tctgctaaat ccaattgttt ctttgctatg ttgatccctc ttgctaaagg tttcattgct
    15181 cgtgcaacat acaaaagatc aaatttttca atcgctcgcg cgacacgttt tgaattattg
    15241 tttgaaacaa caacaacagg gattcctgca tttttcattt ctaaaatcca agtaagcaac
    15301 tcttctgttc catctggatt attccatgca atcaacgtat tgtctaaatc ggtcaatact
    15361 gctttgatgc ctagattctt taattgggca ggtgtaatct gatagatggc ttctaccatc
    15421 catgttggtt tgtaatttga aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac
    15481 ataaagtact cctcaacccc gtattatact gcatatcagc aaaaaaaact acctctatac
    15541 tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac caaaaataaa gattgatcac
    15601 agaaaaattt agaaacaaaa aacaactaat attggcggaa cctcttgtat cgtttttcca
    15661 gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct ttcaatcaac gcttttttca
    15721 taatgtgtgc gactttatat cgtggcaact gctcgccgtt gaacgtttca ggaataactc
    15781 gatcaacgat tcctaactcg cgtagttctg gcgcagtaat tttcatcaac tcagctgctt
    15841 ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa tccttctgga gaaagcacag
    15901 cataaatcgt ttcttccagc atccagactt catccgctaa acctagagca agggcaccgc
    15961 cacttccacc ttcaccaatg atgatggcga tgatcggtac ctttaaatca gacatttcga
    16021 gcagatttct ggcaatagcc tctccttcgc cgcgctcttc tgctcctaca ccgcaatatg
    16081 cacctgctgt attgatcaag gtaatcaccg gtctattgaa cttttccgct tgtttcatca
    16141 gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc aaaatttctg cggatatttt
    16201 cctgcagatc ccgtcctttt tgaataccga tcactgttac cggtttgcca tctaatcgag
    16261 cgattccacc aacaatcgct tcgtcatctg caaataaacg atcaccatgg cattcctcga
    16321 aagaatcgaa gatctcttca aaaaaatcca agcttgtcca tcgttcttta gctcttgcta
    16381 actgaacgac atcatgtgct gtcttatcca cgatctatcc atcctttctg tgtatgcaat
    16441 tcaagaagcg tatggatttt ctgtttcaat tccatgcggg gaacaatctg atcgacaaaa
    16501 ccatgagata ataagaattc agccttttgg aaatcttctg gaagttcctg cttgatcgtc
    16561 tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt ggggttctgc tagaataata
    16621 tcgccttcca ttgcaaaact ggcagttact cctccggtcg tcgggtccgt taaaacagtt
    16681 agataaaaaa gaccttcatt gctatgccgt ttgacagcag cagagatttt tgccatctgc
    16741 attagagaaa agattccttc ttgcatccgt gcgccacctg aagctgtgaa caagacgact
    16801 ggaagacttt cctttgttgc tcgttcgaat aatctggtga tcttttctcc gacaatcgtt
    16861 cccatacttc ccatgataaa attaggatcc atcactccaa taatgaaggg aatcccctga
    16921 atggttgctt taccagtgag gactgcttca tgaagtcctg ttttatcttg cattttttta
    16981 attttttccg tatattcggg aaatgctagc ggatctttgg ttacaagatc cgtatcccat
    17041 tcttcaaaac ttttttcatc aataatcaaa gctagacgct gccatgcacc gattcgaaaa
    17101 gcgtatccgc aatgagggca tactttttct tcaccgatgt ctttcgtata aaggatatgt
    17161 ttgcaatttg gacactttgc ccacatgtta tcaggaacag atggcgagtt agcaggtttg
    17221 ctctctctat taggattgat ccgaatatat ttctttttct taaacagagc catctatgcg
    17281 cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt cttcttgtaa aaatgctgtg
    17341 ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat ccatctgaaa ttctgcgttc
    17401 gtgatgattc cttctgtgat aaattcactt aaggctcgct gcatcttcat taatgcttca
    17461 aaacgcgtat ttccatggac gattattttt gcaatcattg aatcataata tggcgggatc
    17521 gtatatcccg ggtaaactgc actgtctact cgcagtccca tcccacctgc tggaagcagt
    17581 aagttttgga tcttccctgg agaaggagca aaatggaaag caggattttc tgcattaatc
    17641 cggcattcga ttgcgtgtcc ttggaatact acttcttctt gacacacatt caaaggctca
    17701 ccggctgcta tctccacctg tttttttact aaatcgattc ctgtgaccat ttctgttact
    17761 ggatgttcca cttggattcg agtattcatt tccatgaaat aaaaatcgcc ttcttcatcc
    17821 atcagaaact cgatcgttcc ggcattttca taattgactg ctttagctgc tcgaacagca
    17881 gcgtctccta gtgcttctct ttttgtttgt gagatcacta cagaaggaga ttcttccagt
    17941 actttttgat tgttgcgttg taaagaacaa tctcgctcgc ctaaatgaat gacatgacca
    18001 aatcgatcac ctaagatctg aacttctata tggcgagcag gataaatgat tttttccaaa
    18061 tacatatcat catttccaaa tgccgctgct gcttcttgct gagctgaaga aaaatgctgt
    18121 gggagctctt cttttgattg aactttccga atgcctttcc cgccaccacc tgcagctgct
    18181 ttcagcatca ctggataacc aattctgtct gctatttcca atgcttcttc tactgtagat
    18241 aatacaccag tacttcctgg aatgactggg acatttgctt cttgcatcaa ttgacgcgca
    18301 ttgatcttat ttcccattgc atcgatcgtt gcacttttag gaccaataaa tgtgatattg
    18361 cactcttcac acatggaggc aaatcggcta ttttcagata aaaaaccaaa tcctggatga
    18421 attgcttcgg cttttgtcac gattgcagcg ctcagtactt cctgaacatt cagatatgaa
    18481 tcaacggctt ttgccgggcc aatgcagatt gcttcatctg ctaactgtgt atgaagtgct
    18541 tcggcatctg cttctgaata tatcgctacg gtttggatcc ctagttcgcg gcatgcacga
    18601 atgatacgta cagcgatttc accgcggtta gctatcaata ctttagaaaa catatttcac
    18661 ctatccaatc ataaaggtta attccgcttc ggctactttt ttaccgtcta ctttagcgat
    18721 tcctttgcct attccggctg aagctttcac tttcaatatc tcaacttcaa ggtataaggt
    18781 atctcccggt gtaactttct tacggaattt tgctttgtcc aatccgccga aataagccgt
    18841 ctttcccttg aattgttcaa gagaaagtaa tgcaactgct cctgcttgag ccattgcttc
    18901 aacaatcaat acccctggca taacaggttc ttctggaaaa tgtccttgaa aaaagggctc
    18961 attgatcgtt acatttttct tagcaacgat ccgttctcct tcaaccattt cttccactcg
    19021 atcaatcaaa agcattggat aacgatgtgg aatgatttct tttatttctt gtatgttcat
    19081 tgttttccct ctttcctgct ttgcttattt ttgctgaatc ctaaagagtg gctgtccata
    19141 ttcgacaact gactcgtttt ctacaaggat ttctgtgatc actccgtcgg cctcactagt
    19201 tatctcattc atcagtttca tcgcttcaat aatgcagata acttctcctt tggcaacttg
    19261 atcacctact tttttgaaag ctggtttgtc gggcgctggt tgcagataaa cgattcccac
    19321 gattggcgaa acaacctcga ttccatcaaa tactgccgat tctttttctt tcattatttc
    19381 tggagcagtt gttgcttgtt caggtatgac attgactggc tccgaaactg gcaacgtttc
    19441 gtttgtttga accatattca tgcctcgact tgttttattt ttattcatat acaattcaaa
    19501 ttgaccttca cgcaaatcga attccgttag cgaggattga tcaaattgac tgactaattc
    19561 tctgatttca ttgatatcca aaattattcc tcccagcgtt tcatgcacag aaccgcatta
    19621 tgtccgccaa accctaatga attactcatt gcataagtaa aacttgcagg tttgctttcg
    19681 tttttgacaa ctgtcaagtc aatctcagga tcttgttttt ctacattgat cgtaggtggg
    19741 ataaattgat gttctaatgc cagcaaagtt gccaaggctt cgatcccgcc ggctgctccc
    19801 aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag tatctctgaa attttcacct
    19861 aatgcgattt ggattgcttt tgcttctgcc tggtcattgg ctggtgtact tgttccgtga
    19921 gcattgatat atccgacttg ttccggctgt acaccagctt cttccatcgc aagcttcatt
    19981 gctttagatg caccagaacc atctggagta ggagctgtca tatggtaagc gtcacagttt
    20041 gcaccgtatc caacgatttc tccatagatt ttggcaccgc gagcttgtgc atgttctaaa
    20101 gattcaagaa ctagaacgcc tgctccttca cccatcacaa aaccattccg attttcgtca
    20161 aacggaatcg aaccacgatt aggatcagtt gattctgaga gtgctgttaa agctgcaaat
    20221 ccagcaatcc ccatctcaca aatcgttgct tctgttcctc cagcaagaat gacatcagaa
    20281 tagccatgtt tgatgttacg gaatgcttcc ccaattgaat tcgttccgct agcacaagct
    20341 gttacgatag ctgtacaaat accttttgca cctacacgca aagcaatatt tccagctgcc
    20401 atgttggcaa tcgccattgg tacgaacatt ggagaaactc gttttgcacc tttttcatgt
    20461 agacgaataa cttggttttc gattgttgga agaccaccga tcccagatcc gatgatgacg
    20521 ccaaaccggt cttgattcat tttttcttta tctaaaccgc tcatctccat tgcttctaaa
    20581 gcagcgtaga ttccatagat cgaaaacata tccattcgtt tgctatcttt tttaataaaa
    20641 tatttatcgt aagggaaatc tttgacttca cccgctaagg taatccctgt ttctgaagca
    20701 tcaaatttag tgatcggtgc gatgccattc gtcccctctt ttaagctatt caaaaagctg
    20761 tctgcgtcat ttccaatagg tgaagtgact ccatagccag taattaccac tcgattcatt
    20821 tgtttgctct ccttttctta tccgtgcatg accaatccgc catcgacatt gatgacttgt
    20881 ccggtgatat atggattttg tgctaagaaa actgctgttt gtgcaatatc ttctacttgt
    20941 ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct tgactttgtc tgctaaaact
    21001 tccgtcatat cagttgtgat gaatcctggc gcaatggcat tacatgtaat gcctctagtt
    21061 gcagcttctc gtgccacaga ttttgttaat cctactacgc cagctttact agctgcataa
    21121 tttgcttgac caatatttcc aatcaaccca gatacactag agagattaat gatggcccct
    21181 tctcgctgct tcatcatttt tttcaatacg tgctgcgtca tattgaaggt tccaatcaaa
    21241 ttgatatcca gacatttttt gaaatcttct gcatccattc gcatcacaag tttgtcattt
    21301 gtgattccgg cattgtttac taacacatgg atcgatccaa gcttttcttc tgcttctttg
    21361 atcatttgtc ccgctttctc ataatcagag atgtcaccgg aaactcctac gcattttaca
    21421 ccgaatgctt cgatttcttc tatcttttct tttggaatct ctccgcgacc attcaaaatg
    21481 atatttgctc cagcttttgc aaaagctaag gccatcgctt tgccgattcc tcttgtacta
    21541 cctgtgataa atacattttt tccttttaca tccataacgt tcctccttat agtattgcta
    21601 ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac tggtattgtt ttatcgattt
    21661 ttttcataaa tccgcttaat acttttccgg ggccgacttc gatgacttgt tcgatgccga
    21721 ttgtttttaa cttatggata ctctcataga aacgaacagg ttgcatgact tgctgttcaa
    21781 gtagtcccgc gattgtttct ttcttcatta tttccgttgt tgtgttgctt atgataggaa
    21841 aagaaggttc agagaattga atctgactta ggtccttcgc cagttttttt gctgccggtt
    21901 ctaaaatagc tgtatgaaaa ggcccgctga catttagagg gatcattctt ttgaaccctt
    21961 tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc ttcgccacca atcacgatct
    22021 gctgaggtgt attataattt gcaggagata cgatgccgta cttgctagct tcatgacagc
    22081 tttcctcgat cgtttcaatc ggcgcattca tcactgcaac cattttcccg cttccagctg
    22141 gggcagcttc tgtcatataa gcgcctcttt ttgctactaa cgcaactgct tcagtgaagg
    22201 atagcgcatt actagctact aatgccgaat attctcctaa actcagtcct aaagctgcat
    22261 ctgggatgat tccatgctcc ttcaataagc gataatacgc gatgctgact gttagaatag
    22321 ctggctgtgt atattgcgtc tgatccaaac gttcattttc tgtaaagcaa agttcagcca
    22381 tatcgtagcc gagaatctca cttgcttcat caaatgtctg ctttacaacg gcttcttcat
    22441 ataaatcttt tcccatacct tgatattgtg caccttggcc actaaataag aatgctgttt
    22501 tcatgttttt ctccaatata tccagaatat atgtttgatt cttcctattc aaaaaggccg
    22561 agacattcct atttcaggaa atgtcccagc ccccaaccta attttaatga ttatgcagtt
    22621 tgtttttcta cgtaagtcac taaatcttga accgttttga ttccgtcttc tgtttcgatt
    22681 ttcacgtcaa attcatcttc aatttcattg atgatttgga ataaatccaa gctgtcagct
    22741 tctaagtctt cttggatgtc agtagtcaat ttgatttctt cagtttcttt tcctaattct
    22801 tctgcgatga tttcttgaat tttttcgaat accataataa tatattcctc cgatattgtt
    22861 tgctttttat ttttataatt taacagtcta caactccatg agaatacttc cccatgttaa
    22921 acctccgcca tagcctgtaa atattacttt ctgctgtttt cctaaggtga tctttccact
    22981 agaaactgct tcatccaata agatagggat gcttgcagca gatgtattcc catatttatc
    23041 catattggtc aggaactttt cttgcggcat ctttactttt cgggcaattt tctcaataat
    23101 tctgaggttt gcctgatgca aaagcagata atcaactggt ttgtctttca tgacttcttg
    23161 gatattgttt gcaacgtctc gtacggcaaa atcaaaaata tctctgccag tcatttgcaa
    23221 ataatacgaa ccttctgaat cttctgaata gaacggattt ttattttgct ggtagccagc
    23281 ggtcaatgag ctacttctgg taccgtcagc tgctaatttt tctttcagca attttttctg
    23341 tggattcgct tctaagagaa tcccacctgc tccatcgcca aacaacacag cagtcgtacg
    23401 atcagaccaa tcgattagct tagatagtgt ttcaccacca atcaccagtc ccttagttga
    23461 gccgcagcgg atcatttttt cagccatgct taacgcataa acaaatcctg agcacgcagc
    23521 actgatatca aaagcgatag catgagttgc tcccaatttc ccttgtactt gtgctgctac
    23581 agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa ataaaatcca aatctttagg
    23641 gtttagattt ttctgtttta tcaatttttc cgccactaaa atgcataaat cagaagtctc
    23701 ctgatttgtt acacatcttc gttcacgaat acccgtcctt gacgaaatcc attcatcaga
    23761 ggtatccatc atcttggaaa gatcattatt agtaataatc ttttcaggaa ggacgctggc
    23821 tgttgcagta atcattccaa attgttccat taaatatcct cacttatagt cttgcaagaa
    23881 gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt tcctccgtag ccattccgtc
    23941 taagatgttc ttgaccattt cacgatgaaa atgctgatgg acccgaaata gtaactttcc
    24001 ttttttcgtt agtcccaact ttacaacgcg gcggtcatct tcactgcgga tacgttcgac
    24061 atagcctttt tttactaact tgttaatagc agtggtcaag gtcccaacag taattgatag
    24121 ttctttggct acttctgaag ttgttttttt cttgtacatc ccgattgcct cgatcgtgtg
    24181 catctctgtg attgacagat ctttgaattg agattttttc agctcagact cttcgattgt
    24241 caaaatatca ttgaagacac taacaagata atcatttact gtttttaaat ctggttccat
    24301 gttccaccgt ccactaatta gtttgattat caaatctttt gatgttcaaa ctata
//
LOCUS       DAZULG010000030        24257 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000030 DAZULG010000000
VERSION     DAZULG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.030"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..995
                     /locus_tag="ACG9CY_001109"
     CDS             138..995
                     /locus_tag="ACG9CY_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS4937634.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1110..2030
                     /gene="pstC"
                     /locus_tag="ACG9CY_001110"
     CDS             1110..2030
                     /gene="pstC"
                     /locus_tag="ACG9CY_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS4937635.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2030..2914
                     /gene="pstA"
                     /locus_tag="ACG9CY_001111"
     CDS             2030..2914
                     /gene="pstA"
                     /locus_tag="ACG9CY_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS4937636.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2929..3735
                     /gene="pstB"
                     /locus_tag="ACG9CY_001112"
     CDS             2929..3735
                     /gene="pstB"
                     /locus_tag="ACG9CY_001112"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4937637.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3751..4509
                     /gene="pstB"
                     /locus_tag="ACG9CY_001113"
     CDS             3751..4509
                     /gene="pstB"
                     /locus_tag="ACG9CY_001113"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4937638.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4522..5199
                     /gene="phoU"
                     /locus_tag="ACG9CY_001114"
     CDS             4522..5199
                     /gene="phoU"
                     /locus_tag="ACG9CY_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS4937639.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5437..5736
                     /locus_tag="ACG9CY_001115"
     CDS             5437..5736
                     /locus_tag="ACG9CY_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937640.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5777..6469
                     /locus_tag="ACG9CY_001116"
     CDS             5777..6469
                     /locus_tag="ACG9CY_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4937641.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6466..7233
                     /locus_tag="ACG9CY_001117"
     CDS             6466..7233
                     /locus_tag="ACG9CY_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4937642.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7333..8901
                     /gene="liaX"
                     /locus_tag="ACG9CY_001118"
     CDS             7333..8901
                     /gene="liaX"
                     /locus_tag="ACG9CY_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS4937643.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9198..9518
                     /locus_tag="ACG9CY_001119"
     CDS             9198..9518
                     /locus_tag="ACG9CY_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS4937644.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9518..9868
                     /locus_tag="ACG9CY_001120"
     CDS             9518..9868
                     /locus_tag="ACG9CY_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4937645.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10035..10973
                     /gene="hprK"
                     /locus_tag="ACG9CY_001121"
     CDS             10035..10973
                     /gene="hprK"
                     /locus_tag="ACG9CY_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS4937646.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10988..11818
                     /gene="lgt"
                     /locus_tag="ACG9CY_001122"
     CDS             10988..11818
                     /gene="lgt"
                     /locus_tag="ACG9CY_001122"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4937647.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11862..12887
                     /locus_tag="ACG9CY_001123"
     CDS             11862..12887
                     /locus_tag="ACG9CY_001123"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4937648.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12902..13840
                     /gene="galU"
                     /locus_tag="ACG9CY_001124"
     CDS             12902..13840
                     /gene="galU"
                     /locus_tag="ACG9CY_001124"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4937649.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13965..14405
                     /locus_tag="ACG9CY_001125"
     CDS             13965..14405
                     /locus_tag="ACG9CY_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS4937650.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14408..15010
                     /locus_tag="ACG9CY_001126"
     CDS             14408..15010
                     /locus_tag="ACG9CY_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4937651.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15078..16184)
                     /locus_tag="ACG9CY_001127"
     CDS             complement(15078..16184)
                     /locus_tag="ACG9CY_001127"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS4937652.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16394..17395
                     /gene="ccpA"
                     /locus_tag="ACG9CY_001128"
     CDS             16394..17395
                     /gene="ccpA"
                     /locus_tag="ACG9CY_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS4937653.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17731..20076)
                     /locus_tag="ACG9CY_001129"
     CDS             complement(17731..20076)
                     /locus_tag="ACG9CY_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS4937654.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20355..21257
                     /locus_tag="ACG9CY_001130"
     CDS             20355..21257
                     /locus_tag="ACG9CY_001130"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS4937655.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21470..23245
                     /locus_tag="ACG9CY_001131"
     CDS             21470..23245
                     /locus_tag="ACG9CY_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS4937656.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23502..>24257
                     /locus_tag="ACG9CY_001132"
     CDS             23502..>24257
                     /locus_tag="ACG9CY_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4937657.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         7748 a         4334 c         5089 g         7086 t
ORIGIN      
        1 tagcatagaa acttttaatg agatagtttt ttttacaatt atgagaattt attttgttat
       61 ttaatgattt tgattcgttt tcatcaagta atgaaaatga taaaataaac atactttagg
      121 attaaaggag atttctaatg aaaaaactta aattattctt accaataata ggattagcca
      181 ttcttttatc aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct
      241 cttctgcttt acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg
      301 gaaaattcat caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag
      361 gagcagtcga catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct
      421 cagcattagt cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata
      481 ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa
      541 actggagtga agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa
      601 gcggaacccg tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac
      661 aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca
      721 gctatgttgc tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag
      781 aaccgacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt
      841 acacaagaga tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg
      901 aagtacaaga taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg
      961 aacgtgattg gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta
     1021 gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta
     1081 tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa
     1141 aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc
     1201 tgatcgtgtt ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct
     1261 tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc
     1321 aaatcggagc aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta
     1381 ttgtgacctt tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca
     1441 tgacggagat ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg
     1501 taggtattcc ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca
     1561 gaagtatttt tggcgggaca gggtttggta tcttagctgg tacatttgtg ttgttcgtta
     1621 tgatcttgcc gactgtcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact
     1681 atcgggaagc ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc
     1741 tacgagcagc cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg
     1801 gtgaagcctt agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggactta
     1861 ctactcctgc ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg
     1921 gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat
     1981 tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa
     2041 acgctcagat aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat
     2101 tttagcagct ttactacttt atatcttagt tcggggaata ccgcatattt catggtcctt
     2161 cttgaccgaa ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa
     2221 ctcgatttat ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg
     2281 tatctatctt tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat
     2341 tgaaatcttg agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt
     2401 tattcaattc cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa
     2461 cttgcctctt ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg
     2521 agaagcagga ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc
     2581 agaagcgaca ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga
     2641 agcagctgca ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg
     2701 gaatccatta agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt
     2761 ccatatttgg aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg
     2821 cgcttctgcc gtgttgatca ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg
     2881 caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat
     2941 aactggaatg aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg
     3001 gatgatctac atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt
     3061 gaaaaaaata agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt
     3121 cgttcgttga atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg
     3181 tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt
     3241 ggcatggttt ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt
     3301 gctttgaaac agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc
     3361 ttgaaacaag ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct
     3421 ttatctggcg ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat
     3481 atcttgctgc tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa
     3541 gaaacacttg taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa
     3601 caagcagctc gtatcagcga ctatactgcc tttttctatc taggaaaagt aatggaatat
     3661 gacaaaacta acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc
     3721 ggtcattttg gttaagacgg aggcctcaac atgacaaaag aaattatttc atctaaagat
     3781 cttcatctgt attatggaaa gaaagaagca ctaaaaggga tcaatctaag tttcaatcaa
     3841 ggggaaatca ctgcgatgat cggtccttct ggttgcggga aatccactta tctgcgttct
     3901 ttgaaccgga tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa
     3961 gacaaagaca tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg
     4021 gtattccagc agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg
     4081 aaattaaaag gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag
     4141 gcagcgtctg tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc
     4201 ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc
     4261 ttattggatg aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg
     4321 ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct
     4381 tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga atacaacgat
     4441 acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga
     4501 tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc
     4561 cataatcagt tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt
     4621 gcatacatca aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc
     4681 aataacatgg aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca
     4741 gttacaactg atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt
     4801 atggccgatc atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga
     4861 ataccggaaa tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac
     4921 aacgtcctag ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg
     4981 gatcaaagag tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag
     5041 gaaaatcctg agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa
     5101 cggatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa
     5161 atcactgagt taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg
     5221 tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt
     5281 aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac
     5341 cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca
     5401 caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc
     5461 actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc tgctttatat
     5521 gaagtacctg tcatcgagat cgaaaaagta cgaataaaag aggatcatca attgattaca
     5581 ggaagactga tgaacacgga tcaaaaagga gaatcgattt atttatcggt tccttatcaa
     5641 aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat
     5701 ggagatgaat ggcagctagt agcaaaaaaa aaatgatggc tggcttttct tcttcacaag
     5761 tatctccatt ggcgccatgt tgcttattgg cggaaaacag ggtctatttg ctttaggaag
     5821 tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg
     5881 gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt
     5941 ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt
     6001 ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg
     6061 ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact
     6121 gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga
     6181 attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt
     6241 gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc
     6301 agttccatcg cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca
     6361 gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat
     6421 ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt
     6481 atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat
     6541 attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat
     6601 cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc
     6661 ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc
     6721 tttttatttt gtttcctact attgcttcct atcactgatt ttttagcgat ccaaggtttt
     6781 acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga
     6841 ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga tgctagtgta
     6901 gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa
     6961 ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt
     7021 ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt
     7081 ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt
     7141 accacattgt ctgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt
     7201 acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt
     7261 tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag
     7321 gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt
     7381 tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa
     7441 ataaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa
     7501 ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa
     7561 agagaaaggc aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg
     7621 aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca
     7681 cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg
     7741 acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct
     7801 agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt
     7861 aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat
     7921 tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga
     7981 tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac
     8041 aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag
     8101 catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga
     8161 aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt
     8221 tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt
     8281 aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc
     8341 tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg
     8401 attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg
     8461 gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga
     8521 aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg
     8581 acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa
     8641 gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca
     8701 gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg
     8761 tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt
     8821 cgtgtttcag atggtgaaat cacgcaagtg caactttcaa cgacaacagg aagtatctat
     8881 ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga
     8941 agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg ccgataggct
     9001 atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag
     9061 gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt
     9121 ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag
     9181 tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atccaataaa aatgttgttt
     9241 tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg
     9301 tgctttatgt attcctgagc ttagttgctt ttggaagtcc aatcatttta tacattttat
     9361 tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat
     9421 attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag
     9481 cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt
     9541 agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag
     9601 cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa
     9661 accgattttg acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt
     9721 tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag
     9781 tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat
     9841 tgtcagtgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt
     9901 ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt
     9961 tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa
    10021 atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt
    10081 tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct
    10141 cacgcccagg actagagttg actggttatt ttaattatta ttcccataat cgtttacagc
    10201 tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca
    10261 tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccgg
    10321 ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca
    10381 tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc
    10441 gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg
    10501 acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggt catcgattga
    10561 tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc
    10621 ctaaaatcct ggaacatcta attgaaatcc gaggaatcgg gatcattgat gtaatgaatc
    10681 tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag
    10741 catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg
    10801 cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca
    10861 tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa
    10921 catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag
    10981 tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta
    11041 ctggtatgca ttgatcatcg tatcaggtat cattatcgcc atgtggctta gcagcagaga
    11101 ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc
    11161 tatctccatt attggtgctc gcttatatta tgttttattt gatcttcccc agtatatagc
    11221 tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg gcggactgat
    11281 tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt
    11341 tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt
    11401 catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt
    11461 accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct
    11521 ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc
    11581 acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg
    11641 tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc
    11701 tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa
    11761 aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt
    11821 tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg
    11881 ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg
    11941 acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc caacacacga
    12001 atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg
    12061 aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt
    12121 ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta
    12181 aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag
    12241 cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcga
    12301 aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac
    12361 aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag
    12421 agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag
    12481 ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt
    12541 taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga gtaggcgatc
    12601 tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac
    12661 aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata
    12721 ctactcaagc agcgatggaa ctagcccaaa taatggaaat cgaaatgccc atcactcaaa
    12781 caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt
    12841 tacgtgatgg gaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta
    12901 tatgaaagtc aaaaaagcag ttattccagc ggcaggatta ggaacaagat ttttaccagc
    12961 aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat
    13021 cgttgaagaa gcgttgaaat caggaatcga agacatcttg atcgttacag gaaaagctaa
    13081 acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa
    13141 aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat
    13201 ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg
    13261 aaatgaaccg tttgtcgtta tgctaggcga tgatatcatg gaagatgaag taccattgac
    13321 acagcaatta atcgatgatt acgaacgtac tcatgcttct acgatcgccg ttatgcaagt
    13381 tcctcatgat gaaacttcaa aatacggaat tatcaatcca ggagaagtat tggaagatgg
    13441 gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt
    13501 agctattatt ggacgttact tattgactcc agaaattttt gatgtattgt ccgagcaaca
    13561 accaggagcc ggaaatgaaa tccaactaac tgacgcaatc gatacattga ataaaacaca
    13621 acgcgtattt gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca aatttggttt
    13681 tatgaaaaca agtattgaat atggtctaac acatccagaa gtaggtgcac ctttacgtga
    13741 gtatatcctg tctttaggta aaaaattaga agcagaagga agcaagatcg atcataatcc
    13801 aaaaggaata acagaaatca aagaagaaaa gaaaaaataa cccttttttc taacgagctt
    13861 cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa
    13921 aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa
    13981 ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt
    14041 tgttgcaagt ttctaaaact ttagaaaaag tagatcagac tgtagcagaa gcaaatacga
    14101 cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa ggcttgctgg
    14161 tcaaaagtaa tgaactgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt
    14221 tcacagcagt tgctgattta agtgaaagtg tatcagaact taatcattct agtaaaaaca
    14281 tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa agcgacggtt gccggaaaag
    14341 tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa
    14401 aataaaaatg gctaaaaaag gtggattttt attaggtgca gtagtcggag gaacagccgc
    14461 agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa gaacttcggg atgaattagc
    14521 tgcccaagcg gatgatttga tggatatggc ttcagattac acagatatgg cgaaagaaaa
    14581 atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg
    14641 tgatctagca ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag caggaagtga
    14701 atctgacgat atcttgttaa atcttaaaga acaatcagct gacttatcta atcgtttcaa
    14761 aaaaacggca gaagatatga aaaaacctgc agatgaatta ggaagtattg ctaaagatac
    14821 ttcagaagat atcatgattg atgtaaaaga cgctgctggt aaagcaggag atactgtcaa
    14881 tgaaggaatg tcagaagcaa aaccagttgc agatgacaca aaagaaatga tgaaaatggc
    14941 aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat acaatgacag attcatctac
    15001 aaatcactaa aatcactaaa atcacaaatt tatagaataa aaaggtggga gcggactagc
    15061 tctcaccttt tttactctta tttttctaaa actagtaatt cttttggtgt agtcgtgaat
    15121 ggcacacagc catcgcttgt cacatataca cagtcttcga tacgtacccc tactttttca
    15181 ggaatataga ttcctggttc aagagagaag cacatccctt cttcgattac taagtcattt
    15241 ccttctacaa gggaagggaa ttcgtggacg gttgttccga tcccgtgacc taaacggtga
    15301 ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca
    15361 cctgctgtca cacctggacg gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg
    15421 ttgtatattt tttcttggaa gtcggttggt ttttgataag caattgtacg agtagcatca
    15481 ctgcaatagc cattccaaac aacgccaaga tcgaataaca caagttcatt cggtgaaatc
    15541 ttcgtgtttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg
    15601 tcgaaagaca tttgtgaaac accttgtttt ttcaattggt attcgatttc tgcaatgatc
    15661 gcttgttcag tcacgccttc tttcactgct ttaaagccaa tctcaaatgc aacatcggcc
    15721 catttaccag cttcgatcag tttgtctttt tcctctagag ttttgatcag ttgtagacgt
    15781 tggatgatgg aagctagatc agtgtaagaa tccgttcccg tcaagatatc agagatagca
    15841 tcgaagcgct cgacagtcaa ggcgttcttt tcaatcccta cattgccagg ctgaccatag
    15901 cgagcacgaa gttcttttcc gatgatctcc caagggtttt cgctgtctaa atagccacgg
    15961 acatcatacg cccaactact gttttgagca tcttcaactt ccaaagcagg agtgaaaagg
    16021 aaaggatctt gttcaagagg gataaaaaga gctagtacac gctcatgagg atcactttcg
    16081 tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc gacttggtgt
    16141 tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc
    16201 taaattaatt ttgtattcaa taattgtacc actgatttat taaaagacca acgttctaaa
    16261 aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttcttgagat
    16321 tatttgatat tctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa
    16381 taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa
    16441 tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac
    16501 aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaacg cagttgcgcg
    16561 tggcttggca agtaagaaaa caacaacagt tggggtaatc atccctgatg tcagcaatat
    16621 gttctttgct tctttagcac gtggtatcga tgatgtggca acaatgtaca aatacaatat
    16681 tatcttagca aattcagatg gtaacgacca aaaagaagtc aatgtattga ataacttact
    16741 ggctaagcaa gtggacggcg tgatcttcat gggtcaccat atcacagacg aaatccgtgg
    16801 agaattttca cgttcaaaaa ctcctgttgt attagctggc tctattgatc cagatgaaca
    16861 agtaggtagc gtaaacattg attatgtatc agcaacaaaa gatgcagtaa acaaattggc
    16921 aaaaagcggc aataaaaaaa tcgcttttgt cagtggtgcg ttgatcgatc ctatcaatgg
    16981 acaaaatcgt ctgaaaggct ataaagaagc attaagcgaa aataacctac catatagcga
    17041 aggattgatc tttgaagcac aatacaactt caaagatggt ctatctctag tcgatcggat
    17101 ccataatagc ggtgcaacag ctgtctatgt ttcagatgat gaattagcca tcggtatttt
    17161 ggatggttta ttagaccgtg gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa
    17221 caattcacta ctgacagaag tagctcgtcc tcgtttaaca agtatcacac aaccattata
    17281 cgatttaggg gcggtatcta tgcgcctatt gacgaaaatg atgaataaag aagaagtcga
    17341 agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatctacaa aataataaaa
    17401 ggacaaccat tcagaggttg tgagcctgcc gtttaactct gcgttacaag gagcaatttc
    17461 aaaaaacagc atttcatgtt tgatgaatat tgtgactcgt gattcggagt tggcaggaga
    17521 acaccgttta ttcgggaaaa ggggttatga caaaattttg tttaatcctt tttttttgtc
    17581 ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa tccgtgtata
    17641 gatgctgcat tatgttcacc accatcaaaa aaataaggga ctgtgacaat actattgtca
    17701 cagtccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg attctttggt
    17761 tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat agttttcatc
    17821 ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg cccatagcga
    17881 ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt ttccaggtgt
    17941 ttgaccagta aatccagata cttttttctt catcacatca gatgataatt cgaatttctc
    18001 atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat tggcaagata
    18061 attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt gccctgccca
    18121 gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt attcattggt
    18181 agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc ttgagatggc
    18241 atctttgaat ggtgtagtga tttttgcatt gatcacactg cgcatcatgt cattcatgat
    18301 cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat aaattacatt
    18361 tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc cgccgtttgc
    18421 taaagcctga aatccattgg tctgttgaag cgtcgtgaca tctactgtac ctaaaggagc
    18481 agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt agtgctcgta
    18541 agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat aagccgggat
    18601 attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc cttcgttagt
    18661 agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg cataatctga
    18721 tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa caagaactgg
    18781 tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt gattctgact
    18841 gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca tcaatacatt
    18901 tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat tttctacgcc
    18961 attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat atcctttatt
    19021 ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat cagacatatc
    19081 agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat tcatcacagt
    19141 ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa agtcttttgt
    19201 aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt cacgatacat
    19261 actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt ttagcgcacc
    19321 tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga tataagcagc
    19381 ttgcggcagc gtcacatctt ttgcatctac accaaagatt cctcttgcag cttcctggac
    19441 tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca aatacgtagc
    19501 aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga tctcgtttgc
    19561 tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt gctgtgtcaa
    19621 agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc gtaaaacagc
    19681 tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga taattgcagt
    19741 tttcaataaa ggtgaaattt tatcagatga aatagtcgtg cgcatcagat ctgtctggat
    19801 ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa tgtttcctag
    19861 ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat aggcaaaata
    19921 gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag taataaaaac
    19981 agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt ctttttttct
    20041 tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt
    20101 tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt ttttggcagg
    20161 aatttctttt ttaaaagagt taaaacgtga gaaaatgatc ttttttataa aagagttctt
    20221 aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga aaaatcaatc
    20281 attttctttg tatatcatct taccaatata taggaattgt tagtaacaat tgcctttcaa
    20341 ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt
    20401 taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc tatacaggcg
    20461 cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt
    20521 acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc attaatttgg
    20581 ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga gaagagattg
    20641 ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct catgtagggg
    20701 caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag
    20761 aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa
    20821 catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa ttatccgtta
    20881 cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac ttttcaggtg
    20941 taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc catatcaatg
    21001 attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt tttggaacaa
    21061 tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat atttcgaaaa
    21121 tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca tatggaaaag
    21181 aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag ttattagcag
    21241 aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca ggcacattgc
    21301 cttgtccttt tttgtttaag aaacaaagga gtgctttttg cagtcttttt catcatgcat
    21361 cttctccccc ctctttttaa atttagacaa acagggtata ctggtagaaa agtaaaaaaa
    21421 cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga tgctgaacac
    21481 tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc ttggcatagc
    21541 cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg gaacagacat
    21601 gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt tggacggtaa
    21661 atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca gcttattttt
    21721 agtacttgca cataaactgc atctttctta tgcagttttg ttcggcatgt tcagtatttt
    21781 tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt atcttctttt
    21841 aagtttctat ttttattttt tgttttctcc gattagtttt acacatgaat attccactcg
    21901 aacgtatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt gtttactagc
    21961 tatttattac cgtaaatata gttccagcaa aaaaatgctg ccgtggtttt tagggctagc
    22021 tgtttctttt cctttctttt ggttcattcg cgaagattcc atctggcttc ttccttttac
    22081 tgtagtcgcg ttaggcatca gtttcgtcca gatcttgcta ggacaagact tttctttcaa
    22141 agaagcaaag aagttaaaag aacgaccatt tctcctttcg aaacaagaat ggctgaaact
    22201 ttcattgttt ttactccctc tagtcggttt ctccatcgtc tatcagtgga tcgaatataa
    22261 aaacgagaca acatatagta ttgccgccat caatgaccgt tcgagtggcg aatttggacg
    22321 tttgatgaaa cagctgatcc gaatagatga cgaaacgaac ttaaataaag aaaacagtga
    22381 gatatgggtc tcgcatgctg cattaaataa agcattagac gcatctccta catttcatgc
    22441 tgtttctact cgtattgacg aattgtatac gactcatgcg tggacccaag gtggaaaagt
    22501 aaaagaatta ccaggagata tcattttttg ggcattgcga gatgtggtaa gcgaatctgg
    22561 ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca atcaagaatt
    22621 agaagaagcc tatcaaaagg gaactatcaa aaagaaaaaa gaattctatc tgatgggatc
    22681 aggggatgga aagacgatga atgatctgcc aatcgtattt gcttttctga aatcaggata
    22741 tgcctatgca tggcgataca aagatttcca tcaagggggg aattatagtt tcggtacgca
    22801 agaacaagtc gaacaggccc aagaattatt gaatcttcca ctaatggata attggatcga
    22861 tagcagtgat ccgagaacac ctgatttcca gttgacaaaa acagcaaaag tcgcgaatgt
    22921 catgataaaa atttaccaag ttctggccat tcccgtgctg attgcttcaa tagcagggat
    22981 attacttatg atactaggaa ccgcatttgc aaaagctgat cgtgcctatt atagttcctc
    23041 tttgattgta gtcaccggat taatactgac acaattggtt tttcttttcg gcgtctcgtg
    23101 gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca ctggcgcagg
    23161 cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta aattgaatct
    23221 gaaaaaagct ttatcttcca aataaaaaca tactaagatt agaggagaac atcattgacg
    23281 aatgatgttc tcctctattt tctattttta gattatagtg aagagtgaaa gaaagttcga
    23341 acaaagcctt gaggactatg agtccctatc aaaaaccgaa cttctttact gtcattttag
    23401 aatcaggttt acgtatgttg gggccatgag cccgtgtata ggaaattgga atcgaaatga
    23461 agcagtaaag ttctttcaaa acttaaaaag gaggaaaaca aatgccttac attcttgcat
    23521 tagatgtttc aatgggacac agctacagtg tcctatacaa agacgacacc tgcttatttg
    23581 aagacgagat cgaacacact caaagaggat ttcatgcatt actcgaagag atccagcact
    23641 tgcctgaacg tccattgatt gtttttgaag caacggggat ttattccaga ccagtcgaaa
    23701 agttctttca agatcatcac ttttcttact gtttgttaaa cccgttagaa gcgaaaaaac
    23761 agatggaaga aaacacctta tgcagttgga aaacagataa atcggatgcc catcggctcg
    23821 cacagactca tcttaaaaat caacgtcagc caaaagaagt acaaagcaat ttttacttag
    23881 agatgcgcga tttagcccgt ttttatcaag aaattgagaa gaaaatcact cgtctgcgca
    23941 tggatttaca caactgcctt cagttgactt ttccagagct tgaacagttc tttttaaaca
    24001 ggctgacccc ttacgcattg atattgatcc gtctttttcc ccatccagat tttgtattgg
    24061 catctactcg gacaaaaata aaaaataaac tgatcaacga aacacggaaa aaaatctccg
    24121 cgaatcgtgc cgagcaaaag gcagatcaaa tcatccacta tgcacagtgt gcctatccag
    24181 ctgttgaaaa agacagtatc cactgtcaaa aaacgatcta ttacgctgaa cttctccaag
    24241 atttactcga acaaaaa
//
LOCUS       DAZULG010000031        24147 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000031 DAZULG010000000
VERSION     DAZULG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.031"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG9CY_001133"
     CDS             133..3660
                     /locus_tag="ACG9CY_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS4937658.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG9CY_001134"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG9CY_001134"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS4937659.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG9CY_001135"
     CDS             7448..7789
                     /locus_tag="ACG9CY_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4937660.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG9CY_001136"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG9CY_001136"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS4937661.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG9CY_001137"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG9CY_001137"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS4937662.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG9CY_001138"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG9CY_001138"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS4937663.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG9CY_001139"
     CDS             13000..13737
                     /locus_tag="ACG9CY_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4937664.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG9CY_001140"
     CDS             13751..14566
                     /locus_tag="ACG9CY_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4937665.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG9CY_001141"
     CDS             14579..15226
                     /locus_tag="ACG9CY_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4937666.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG9CY_001142"
     CDS             15242..15898
                     /locus_tag="ACG9CY_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4937667.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG9CY_001143"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG9CY_001143"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS4937668.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG9CY_001144"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG9CY_001144"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS4937669.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG9CY_001145"
     CDS             18240..18758
                     /locus_tag="ACG9CY_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4937670.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG9CY_001146"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG9CY_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS4937671.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACG9CY_001147"
     CDS             complement(19645..20565)
                     /locus_tag="ACG9CY_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS4937672.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACG9CY_001148"
     CDS             20691..21743
                     /locus_tag="ACG9CY_001148"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS4937673.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG9CY_001149"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG9CY_001149"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS4937674.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG9CY_001150"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG9CY_001150"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4937675.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ACG9CY_001151"
     CDS             complement(23383..24144)
                     /locus_tag="ACG9CY_001151"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4937676.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
BASE COUNT         7989 a         4217 c         5105 g         6836 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataac
//
LOCUS       DAZULG010000032        24083 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000032 DAZULG010000000
VERSION     DAZULG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.032"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..696)
                     /locus_tag="ACG9CY_001152"
     CDS             complement(334..696)
                     /locus_tag="ACG9CY_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937677.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="ACG9CY_001153"
     CDS             complement(770..1150)
                     /locus_tag="ACG9CY_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS4937678.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1232..2407
                     /locus_tag="ACG9CY_001154"
     CDS             1232..2407
                     /locus_tag="ACG9CY_001154"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS4937679.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2484..3068)
                     /locus_tag="ACG9CY_001155"
     CDS             complement(2484..3068)
                     /locus_tag="ACG9CY_001155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4937680.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3269..4267
                     /locus_tag="ACG9CY_001156"
     CDS             3269..4267
                     /locus_tag="ACG9CY_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4937681.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4379..4888
                     /locus_tag="ACG9CY_001157"
     CDS             4379..4888
                     /locus_tag="ACG9CY_001157"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS4937682.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4918..5577)
                     /locus_tag="ACG9CY_001158"
     CDS             complement(4918..5577)
                     /locus_tag="ACG9CY_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS4937683.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5579..6343)
                     /locus_tag="ACG9CY_001159"
     CDS             complement(5579..6343)
                     /locus_tag="ACG9CY_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS4937684.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6351..7028)
                     /locus_tag="ACG9CY_001160"
     CDS             complement(6351..7028)
                     /locus_tag="ACG9CY_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS4937685.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7065..8450)
                     /locus_tag="ACG9CY_001161"
     CDS             complement(7065..8450)
                     /locus_tag="ACG9CY_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS4937686.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8660..8744)
                     /gene="ffs"
                     /locus_tag="ACG9CY_001162"
     ncRNA           complement(8660..8744)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG9CY_001162"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="ACG9CY_001163"
     CDS             complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="ACG9CY_001163"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS4937687.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9389..10246
                     /locus_tag="ACG9CY_001164"
     CDS             9389..10246
                     /locus_tag="ACG9CY_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4937688.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10327..10662)
                     /locus_tag="ACG9CY_001165"
     CDS             complement(10327..10662)
                     /locus_tag="ACG9CY_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937689.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10729..12099)
                     /locus_tag="ACG9CY_001166"
     CDS             complement(10729..12099)
                     /locus_tag="ACG9CY_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS4937690.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12177..12713)
                     /locus_tag="ACG9CY_001167"
     CDS             complement(12177..12713)
                     /locus_tag="ACG9CY_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4937691.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13022..14503
                     /locus_tag="ACG9CY_001168"
     CDS             13022..14503
                     /locus_tag="ACG9CY_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4937692.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14606..15313)
                     /locus_tag="ACG9CY_001169"
     CDS             complement(14606..15313)
                     /locus_tag="ACG9CY_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS4937693.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15392..15730)
                     /locus_tag="ACG9CY_001170"
     CDS             complement(15392..15730)
                     /locus_tag="ACG9CY_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS4937694.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15841..16803)
                     /locus_tag="ACG9CY_001171"
     CDS             complement(15841..16803)
                     /locus_tag="ACG9CY_001171"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS4937695.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16818..18149)
                     /locus_tag="ACG9CY_001172"
     CDS             complement(16818..18149)
                     /locus_tag="ACG9CY_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS4937696.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18157..18837)
                     /locus_tag="ACG9CY_001173"
     CDS             complement(18157..18837)
                     /locus_tag="ACG9CY_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4937697.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19056..19535)
                     /locus_tag="ACG9CY_001174"
     CDS             complement(19056..19535)
                     /locus_tag="ACG9CY_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS4937698.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19662..21341)
                     /locus_tag="ACG9CY_001175"
     CDS             complement(19662..21341)
                     /locus_tag="ACG9CY_001175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4937699.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21889..23391
                     /locus_tag="ACG9CY_001176"
     CDS             21889..23391
                     /locus_tag="ACG9CY_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4937700.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23404..24066
                     /locus_tag="ACG9CY_001177"
     CDS             23404..24066
                     /locus_tag="ACG9CY_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937701.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
BASE COUNT         7223 a         4944 c         4080 g         7836 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa cataatacct
     3121 aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta
     3181 taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct
     3241 gatatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc
     3301 aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat
     3361 cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat
     3421 ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa
     3481 acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg
     3541 tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat
     3601 tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc
     3661 atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa
     3721 agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat
     3781 tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt
     3841 atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc
     3901 aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat
     3961 tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct
     4021 ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac
     4081 ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact
     4141 gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa
     4201 tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc
     4261 tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt
     4321 tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat
     4381 ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt
     4441 aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt
     4501 gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa
     4561 cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc
     4621 gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt
     4681 gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt
     4741 gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt
     4801 agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc
     4861 agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta
     4921 gacgagttct ttcttttttg tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc
     4981 aaaaaataat tctaataata caaaaaataa gataaagcta tgcatgaaaa attcttctgg
     5041 aagtacattg atgattggat cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa
     5101 cgtttcatga aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac
     5161 aactggcagt aacatgccga tttgaaacgg acgaatcaac cgccaaagcc gatcattctt
     5221 ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca cagcataatc
     5281 cactaaaaac aaaattttca cttcataaaa gtgatgggct ccgctagcag aaactgggaa
     5341 atctggcaag ctgaggatgg ttttgaaggg attattgaga taattcatta ggtgatcaaa
     5401 atttttcagt aacgtctctt gatctaaaga agtataatcc aaaatttgca aatgtccgat
     5461 atcaaatacg tatagtggtc gaaagttgat cgtcacacca actgccagac tgatcagtgt
     5521 cagaaatagg ctgaacaatc cagcatattc catccactgc catctcgaat ttttcatatt
     5581 agaagctcca ttcatccagt gattgtttca cataggtagc tgggataggc aacccaggta
     5641 catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt atctatatta ttgcgtatcc
     5701 cagcttggat atctgtttca tagttatctc caaccatgat tacttcttct ttttgcagcc
     5761 ctaatacttc aacggcttta tccataatga tggcttctgg ttttccgatg tagatcggcg
     5821 gcgtttgggt tgctgtctct acaaaagaga tgactgaccc tgctccagga agaaggccgc
     5881 gttcagtagg tatattctta tctgggttcg ttccaatgaa agtagcacct ttttgaatag
     5941 caagtgttgc tttaactact ttttcatagg tcagataatt atctaagcct accactacat
     6001 agtcaggagc ctcttcttcc catgtaaatc cggcagctaa aatcaagtca atcaatcctg
     6061 cttctccgat aacatagact tttttccctt ttccatctgc tttcataaaa tcgattgttg
     6121 ccaaagtagc tgtgtagact gtctctggag ctacatgaat gtcaaactca tctgctagac
     6181 gattagctac tgtttctggt gacttagtcg tgttattggt aacaaacaag aaaggtaatt
     6241 ttcttttttg caattcttcc acaaatcgtt taccggccgg tatgggttgt gtcccacgat
     6301 aaatcgttcc gtctaaatca attaaatatc ctttatagtc cattcatcca ctactcttcc
     6361 ctttttctga tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg ttcttgtttt
     6421 tttacttttt cagaggtttt tgctgactct gtttttcgat ttttaataac cggttttttg
     6481 cgattagatg cgactggtcc ttttttctca tcgatatacg cacgattatg gttttttttc
     6541 ttttttcggc gctgagttgt cttttctttt ttaccgccga ttctctcgat gacaaaatac
     6601 gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct
     6661 ggatgcgccc gtcgattatc cgcttggaaa aatcctttta ggcgtagttg ttcataaccc
     6721 caatctccta cgatataatc atatcgggct agtacatcac tgtaacgttc tccaagcttt
     6781 tctgcattaa atccttcacg ataatcggac acaagccgat aatgtctttc acctatcaaa
     6841 aaattactgt catctaacag tgtcactagc tcgcctttga ctttttctgc tttaggctcc
     6901 tcaacgattt cttccaaagc agctgtcagt tcttcggtga ctgttgtttc ctgttcagct
     6961 gatgattttt tcttagcaat ttctgtttta tgctcaacgt tctgctgttc attttgtttt
     7021 gcagacatgc gttcaccacc tatctttttt attatacctt gtttttattt gattgggtag
     7081 tgagctttca agtaatctcc taccacactt cggataaatt cagggaacaa aaattcattt
     7141 tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta
     7201 aaagtataat ttttattcag atcgattggc ttgttcttcc aaaagacttg atggttaata
     7261 gaatcatatg tgattccatt atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt
     7321 cccttcatag gatagttacg taaaaagtta cgattttttt ctatttctaa aactaagcgg
     7381 atcagatcat ttccacccaa tgtgacattt aacaagtgca tcggatgcgg caacgcagtg
     7441 tgaagctgat cctgattgac ccgccctttt ggaatttcag ttaaaaacag accactgttt
     7501 aatattgctg cttccgtctt tcctcgttct ttcaccgcct caaaggctac ttggataaac
     7561 gaatgctcat tgaagatatc tagactcaga gaaaatggca aatctgctac ttctttttct
     7621 tttaataatt catgcccacg ttctagataa ctatttattt cttgttcatc ttctgtaaaa
     7681 gctgtcattg tttcggtagg gatggctttg gctgaatgct taaggatttt tttctcttca
     7741 tcgatggtta aatggacttc accgacatac tgcccgtact ttccagcggc tgctatttgg
     7801 acatcattga ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc taaaataaca
     7861 tcaattgctg gtatttcttt tgcaatttgg aaatcatcct gaatcccgag atgactcatc
     7921 aaaactaaaa catccacttg tgaacgtaaa tcttcgacta attctggcaa aatatcataa
     7981 ggattccgta tgtcccagcc attcggatta taagtaagcg gaaatggagc agtaaatgca
     8041 attagtccga ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc
     8101 ggtggctgga gcgtattttt atcgaataag ttatccagga tgatatcaaa ctcagcatga
     8161 tcgtataaat gattcaactc ttctttggaa tttcctacgc cttcattgtt tcctatcgtc
     8221 gctgcatcat aaccaacttc attcatcagc aagatatttt cctgtccatt cgttgcttct
     8281 gacaatggat gccaacgatc cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt
     8341 tctttctttt cgttttctcg tttacgttga tccaaaaacc ttctgatcct tggccaattt
     8401 tctaaatgag aatgcagatc attcgtatgt aaaatcacta ttttttccat gtaccctctc
     8461 gcctctcttt atctttatca taccataggc agctttttct tccattgaaa caagctctat
     8521 ttcagagttt ttcctgcctt gaagcaaatt gtataagtac aatattcgat aaatcagtct
     8581 ggctaaaaaa tcaggaacat tcatttttac agtactataa aaataaaaaa aacagacccg
     8641 aaagtctgtt gtcgtttcat tataatcaga cacatggcgg aacctgctta gtgctgcttc
     8701 cttccagacc tgacacgctt cacaagccca ccattgtcct agcctttcgg caaagaatag
     8761 tataccttat ttttcattcg ttgaccagtc tttttttaat ttcttttttc gctggcggag
     8821 ctttcggaaa aagtcagtca aaatcatccc acattcgtct tctaaaatgc caccttctac
     8881 atatgcttgg tggttaaacc gctcgtcctc caaaagattc atcaaggtac cggctgttcc
     8941 gccttttgga tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc
     9001 gctgcacata ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca agcgccagct
     9061 tcccactttt tcacaggctt ccttgattgc aagcatttct gcatgtgtag tagcatcttg
     9121 cgtggtttct ctaaggttat atcctcttcc aatcactttt ccatccaata caaccactgc
     9181 tccaatcgga acttcatgaa gcatttctgc tttctttgct tctgccaatg caaggcgcat
     9241 ccatttttct ttttctactg catcaagatt tgtatttacc aattaccctt ccctgccttt
     9301 gttttttctt gttttacttt agcatatgat aaaaaggctg taactaactt ttttttctat
     9361 ggtaaaataa atacaagagg tgaacaagat gaaaatggat aaattacttg ctctttaccc
     9421 acaagcacag aaaaaaaact ctccagcatc agaagcggat atcctttctt ttgcaattga
     9481 tgatgctttt atctggatca aacaagattc attatctcaa caagaaacca gccttttgaa
     9541 agcgttattc cctgtaatca acgatcaaaa aaaacaccca tggtatcact atctttttaa
     9601 agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa
     9661 aggagaattc ttaaaaaaag aatggcaaga tactattttg gaattatttc gctattctgc
     9721 tgatttcttt ttttatacag acacagatgc gctcttagtc gaacaaataa accgtgatta
     9781 tcttgatgct actgaaatca acggtatttt tctatcttta gatgctgatt ttgatacgac
     9841 aacgtctgta ttcgttggtt ctttccattc acctggcacc gacctcgtcc ctttgtttgc
     9901 tgaagaaaga aaaatttttt taacagagca aaacaatctt tatactcaca gccgtacttt
     9961 ttctatgcaa gatgtggctt tacattacta tacaaaagat actgtaaaag atagtaagtt
    10021 gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt
    10081 atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac
    10141 tctaaaatat cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa aaaaagccaa
    10201 cgatttactg ttttgctatc ttttattgct tcatgaacat aattaaagat agattaaagg
    10261 ggttgtgaca ataattttgt cacaacccct tatttcgaat gaatagtgtt caaaagctag
    10321 tttctcttac atcatttttt caacaaaatg cattagccgg tcttttacat cttcggttga
    10381 atctttgatt gaatcaccat aaacagagat tttctttttt cctgatttga tttttgctaa
    10441 cattcccatc aaagaatcca agtcactatc tgaaagacca tctacgattt ctagtaattt
    10501 ttcatttcca tcaaatttgt catagacgaa tttttttact ttcgtccgag tagtcatatg
    10561 atggatcttt tccattactt ttcccgatac aacgactgct gtcgctacac tggcagcggc
    10621 tgctacgctt aatccgattg ttacttttgt tgatgttttc ataagtatca gctccttttt
    10681 atctatctcc atcatagcat agacaaaaaa ataaacgaac taatccactc agtgaaatct
    10741 caatccttta gggaaaaagt tctttactgt tccttttact tgtttcccaa atccgacagg
    10801 tatcttatct aatactagca aaacccatcc ttgattccca tcacgttgaa atgtttctcc
    10861 tgctgtgtaa gattgccatt ccttttgagt gatcggcaag caaggtatat tctctatctt
    10921 ttttgtagca agcgctaatg catagctagg ttcaaatcga ttctttttga aatctcctaa
    10981 atgcaaacct gtacgtacga cttttaaacc atcgagagaa ggggctaact caggaacttc
    11041 ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct tcataatgaa aatcatttga
    11101 aaactccgtc cagagttttt cctgctcttt tgtcatttgc actttacttt tttttcgtgt
    11161 cttcttttct ttatgcatct gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc
    11221 ttcaccctga tctttatgag gccatatacg tattgttttt tccaaaccag ctacgcttcc
    11281 ccattcagaa cgtcctgaac taacggattg agtcaaagga atctcctcaa tcgttactgg
    11341 ataattttct acaagccagc taatgatctc ttcattttct tctggtgcaa acgtacaagt
    11401 cgaataaatc agttgtcctt tattttttag catttttatt gctgaagaca aaatctcttg
    11461 ctgacgtttt tgacagtata gcggagattc ttctgtccac tcttttattg catttggatc
    11521 ttttcggaac attccttctc ctgaacaagg agcatcaacc acgatccgat caaaaaagcc
    11581 cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc
    11641 ccatcgttct atattttctg ataaaatctt cgcacgtttc ggaaagatct cattcgtcac
    11701 taataaccct ttccccttca tttgagcagc taattgggtt gatttaccac cgggagccgc
    11761 acacaaatcc aaaacttttt caccgggttt tgctgcagca gctgttccaa cgatcattgc
    11821 acttggttct tggctatatt catacccagc ttgatggagg aaagactttc cattgacagt
    11881 gccaagaaat ccctcattgc tgtagggagc gggttgtaat tcttcgctat ggtaagtttg
    11941 gaccatgtcc aatcctgccg gttttagtgg attccaccga aatccttttt tcacgcttcc
    12001 ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac gatatttttt
    12061 gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt
    12121 ttacaatctt ttttacgaaa aataaaggtg gctgatctac tttttcatgt tgctgcctaa
    12181 ggtgactgtt cctttaagat attttccaag tattcatccg tcacctcgtt tttatcccaa
    12241 acagcgttca tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa acttcccaat
    12301 gcattaagcc ataccaattc aagcgacttt tcttctttga tcttcgtgtg acctttgaca
    12361 tatgagaaaa atggccgctt aaatagcgaa tccatgtcta aatcagattt tgcaatgatt
    12421 tcacgaatac tttcatggat agaaaaaaaa tctttcaata agtagacaat gcttaaaaca
    12481 tcatcggaac tgcattcagc taagtagttt tcaaaataaa cagtgatttc ttctaatatc
    12541 ttctctttta acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg
    12601 cagaactctg ctaactctct tgcagtaatt gacttcattc catattcttc cacgtatacc
    12661 atacttttga acagcaaata ttctttacta taatttgtta ctctccccat cattcttacc
    12721 tccgtctctt ttcatctacc cataaataaa taacataaat aattctaaaa atattattga
    12781 taattatcaa aacatattta gttatatcca ttaaaaatca atttttccgc cttaaaaaca
    12841 agaatatctt gacaaaatac cctaggaaat aaacaataga ataaacaagg taacaaaaaa
    12901 attaaataac gttatataaa aaagaaggcg acactctgtg agaataacac agactgaaat
    12961 gactttccac tcacagtttt tgtctaacta tatacaaaaa cagaataaaa aggaggtatt
    13021 tatgctaatt gaagcaagaa aaaaagagtt acttgttcta caaatggtca ttcaaaaaaa
    13081 taattgttct ttacaagaat tatccaatga cttacatatt ccaaaacgga caatcaaaga
    13141 actgatccga aaaataaatg tatcactcaa gcaattttta ttgattgatg aatttattta
    13201 ttctaatcat aaaggagaga tccaaaccaa agaaaccgat caaactaaaa agttagctgt
    13261 tttttctaag ttaaaattat gctacctaaa agaatccaat cgttttaact atttgatgtt
    13321 actaatcaat tatccacaaa ctgctgtacc aaaaaaatat ttacttgaac aactttatat
    13381 atcgccctcc tatctcgaaa aactgacaaa acagctcaat cattccttaa taaaattcca
    13441 tttacggatc ctatctgtaa aaaattgctg tactcttgaa ggaaatgagt tatctatccg
    13501 tctctatctt tacttttttc tttcagatac atttggaggt aacgattggc cattttcctc
    13561 acttgaatta aatgcactaa aaatgaacag tacagatgag caaatagaaa aaatttcaaa
    13621 acaagaaaaa aatatctact tgctgttaac tttattcata ttacgaaaca aacaacaggc
    13681 atattttcca aaaacttctt ctgaaatcac tgaattgatg gacttattgc agcatacaca
    13741 tgattattca gaatacttta cttctttttc agtgagtcgc ccttgtgaag agacagaaac
    13801 agagagagcc tactttaatt attttttcta ttgttttttt ccaaatcaaa tctcttttcg
    13861 acaaaaacag gagattggtt ttttattttc cacaagcaaa aaccttattt gtcgacaagt
    13921 cggtcaattg atgacttact taactcaaca gtttcctctc aaccatttag aaaataaaat
    13981 accaatttat ttttattttc ttgtgttggc agttgttttt ttcgaactgt tgggtgaatc
    14041 cagcagtagt ttttctacac tttgctttcc agaagccacg tccaatcatt ctagttcttt
    14101 tatacaggaa aagaaattaa gagaaaaaat cgatacaatt ttttcaaaaa atggggaaag
    14161 aaaccaaatc agtcaatacc ttagtcaatt gatccaacac ttactacaaa cagaaacgaa
    14221 agagcagtta ctcatccatt tgcagataac caaacagttt acaggaaatt atcatcttca
    14281 aagccatttg cagcagttat ttcgcaaaga tgctgttaaa attaccgcat attcagaaca
    14341 tgctgatttg atcattacag atcagcatga gaaaatttca gatgaaacgg agactttcta
    14401 tctggataca acgcacaata cagtgttatt tggcaagtta ataaaagcgg ttcatgaact
    14461 gtatatgaaa aaaggttatg agaacaaaac aaagaggatg tgacaaaatt cttgtcacat
    14521 cctcttattc caaataaatg gtattcaatc agctaactct cggtattagc tcagatcaaa
    14581 gcgctgattt cacaacttct ttattttaat ctcttcggtt gccgccgaac aataagatca
    14641 agcgcaacag ctgcaggaat gtcgaaagcg ctgctgcaac ataagtcagt gctgctgcaa
    14701 ataatacgtg tcttgccatc ggtacttctt cttccgtcag tagtccgcct tcactcaaaa
    14761 tttggattgc tcgccgagac gcgttaaatt cgactggtaa tgtcaccaat tggaaaagta
    14821 atgctaaaga aaaagcgaaa atcccgatat tgattaacgt agagttccag ctaagcaata
    14881 cacctaccat aatcaagggg aacgaaatca tcgaaccaaa gttagctgct ggaacaagtg
    14941 ctgcacgaat cttcaatggg acataacccg aagcatcttg taccgcatgt ccgcattcat
    15001 gagctgctac acctatcgca gcaactgaag tagactgtgc agttgcttga gacaaactca
    15061 acatcttatt acctgaattg tagttatctg tgagatctcc ggcaatttgt tgaacaccga
    15121 cattatttat ctgttgactt tgtaatatat attgtgctgc ttgtgtacca gtcacattgt
    15181 ttttgcttct cacttgatcg tattttcgga aagtactatt gacgtacgct gacgcaattc
    15241 cggaaataac taatccaaga atgaccaaaa gaaatgtcgg atctaaaaaa ccatacattc
    15301 cataaccata cattttcgtt tcactccttt tcgtttactt tattttatca ttagtagtag
    15361 gaaaagaatg tgaaaagacc ttgaatattc tttaattctt tttcacaaat tccgatttca
    15421 gtttcatcgg tccaaatcct tctaccttgc aatcgatatt atgatcgcct tctactaaac
    15481 ggatgttctt gacttttgtt ccttgtttaa tggcacctga cgcacctttt actttcaagt
    15541 ctttaatcac tgtcacgcta tcgccctcta acagacggtt tccatttgca tctaatacca
    15601 ctaatccgtc ttctatgaca gtttcttgtt cttctttgtt ccattcgttc ccgcattctg
    15661 ggcaaatcaa caagcctcga tcttcatatg tgtactctga gccacattct gggcaatttg
    15721 gtaatgtcat cttctttctc ctttaaaatc tttagtgagc actattgtaa cggaaaaaga
    15781 agatcttgaa aagatacatt tgtgattttt actacaaaag gcacgttttc gaacgaagta
    15841 tcattctgtt tttctgctct tatacgattg gcatatatct tggatctctt tatagatttc
    15901 ctctacttct gctaaatctt ttgcattcgc accacttgct aatggatggc ctcctccatg
    15961 atggcgttta gccaattcat tgatgatcgg ccccttagaa cgtaaacgta cgcggtaata
    16021 ccctgaaggc tgctgaacaa aaatccccca agccagcact tcgtcaatca ctccaggaag
    16081 tgaaacgatc gaagctgtct ctgaatcatc gattccatat ttatcaagaa tttcttgcgg
    16141 aaggattatt cttcctgcac cattttcatc cacctggata ttttgataaa gataaccgga
    16201 aagtttagcg actttcatcg gcatttgttc aatctctctg ttcaaagcac ttgcatcaaa
    16261 tggataattt ctcaatatcg aagcaatctc taatgtacgg gacgtcgtgg agggatacaa
    16321 aaagcgccct gtatctccga cgattcctgc ataaagtaac cgtgcagcct taacgttcat
    16381 ctttaaagat tcaggaaaca tcaaagcaaa ttctgcaatc atctcactac agctacttgc
    16441 ttttgtattt acccacacaa gatctccata agggtcatca ttgggatgat ggtcgatttt
    16501 gatcagtttt tctgccaact gatatctttg atcactgatt ctgggtgaat tagctgtatc
    16561 agtcacgatg actagcgctc cttggtagaa gacatcttca acttcttgca tttctgctaa
    16621 aaagcgaagc ccttcaatcg tttcacctac ttgacgtatt tctttttctg ggaaactttc
    16681 ccgtaatagt tcggctaatc ctacttgtga tcctaatgca tcaggatcag ggcgcaaatg
    16741 cctgtggata atgattttat cgtatttttt tattgtatct actatttctt gctgtactgt
    16801 catttcactc ctgctttcta agaacgttcc attacttgac aaacaacgat tgctttggaa
    16861 acgatggtgt tttcaataaa cacttcgata tccagtttag cggaacgtct gccgatttcc
    16921 aatacacgtg gacgaatgtc taattcgctt tccaattgga tcagccgcaa ataatgtagg
    16981 ttcacttgtt caatcaagat atttcgtttt tggttcatca ccattgtttt ttgtgcgaca
    17041 tcagaaatga tttcgctaag tacgccaaaa gaaatggttc caacgccatt gaccatttgt
    17101 ggtgctacta cgaatttgaa cttagggttt tccaaacgat ttccttcacg gtcagtatca
    17161 atcgtatgga tatttccaga tatctgatcc gaaatcgtat ctgcaatctg cggttgtcgc
    17221 tggacaagct gcatcgcttt cattacatct tgccgggtaa tcaagccttc caatgtcaag
    17281 tcatcggaaa cgaccggcat gacttctagc ccatcccaga tcatttgatg gctaacagag
    17341 gcaatactca ttgttttctt tacactgatt ggttctttcg tcatcacccg atcgatgatc
    17401 tggtttggcg atttccctaa aacatctttt gccgtaacaa tccccatcaa acgatgatgg
    17461 cggttgacga ctggaaaacg cgaatgctgc gtttcttctg acaaacgctg ataatcagaa
    17521 atcgtatctg tctgctgcaa ataatgagtc ttttcagctg gcatatagat atcactgaca
    17581 agcatgatat cttttttgat caattggtcg cttaacgctc ggttgatcat ggttgcgact
    17641 gtaaatgtat cataagaggt gcgtaagatc ggcatttcaa gatcatcagc tagctttgca
    17701 atctcagcag tcgtattgaa tcctcctgtg atcaatactg cggcgccatc ttccaatgct
    17761 aatttttgga cttccgcccg gttgccgacg atcattagtg atcctggcgt aatatagcgt
    17821 tccatggcat gtttagtcat cgcgccgatg acgaatttat tcaagacctt gtctaatcct
    17881 gccgccccgc ctaaaacgtc cccttcaatg atcctgacga cctcgccaaa tgtcaaacgt
    17941 tcaatatgtt tttttaattt gcgttcgata cggatggtcc ctactcgttg aattgttgaa
    18001 actagtccga cattttctgc atctttgatt gcacggtacg ctgttccttc actgacattc
    18061 aattcttttg cgatgcttct tacagaaatt cgattgccaa caggtaaact ttcaatatat
    18121 tctaaaatca aatcatgttt tgtcgccatt ttctgcctac acttccagcg tttccgagac
    18181 gctcattatt tttccttcac tatcttccag taactctaca aaagattcgg gatcttgttc
    18241 aatgacaggg aaagtattgt aatggattgg aactactttc ttggcacgaa ttgtttgcgc
    18301 agcctttgct gcatcttctg gccccatcgt atagttatcg ccaatcggca agaaagcgac
    18361 atcaatatcg aattcttctg caagcaatcc tagatcactg aaataagcag tatcccctgc
    18421 atgataaatc gtttttcctt ctgcttgaat aataattccg ctaggctcgc ccatatactg
    18481 aacgataccg tctacttcat agcttgaact atgctgtgca tgtacgaatt ttaccacgcc
    18541 gaatggaaac gcatgttttc caccgatatt cataccgaat gaatctactc ctctgctgtt
    18601 cgcataatca gcaatctcaa caatactgat aattgtggct tgattttctt ttgcaatagg
    18661 aatcatatct cctatgtggt cactatgacc atgagtaatc agcaaccaat ctgtctttac
    18721 ttcttctgca gttaagtctg tcaatggatt tcctgtaata aacgggtcaa tcatcacgcg
    18781 ttggccattt tccatttcaa ttgttaaaca ggaatgtcca tgatacgtaa ttttcatatt
    18841 ttacacctcg cgttttctct gtatcttttt tattaaaaac gatttcatca cttgcacatc
    18901 aatgaaaaaa tatagctgct aaatatattt tcattggatt gatagtacag tatgtacaaa
    18961 gcaattgtaa cagataatac aggatgcacc aagtaccaag cagatataat ctcaagtttg
    19021 aggtctctgc tctgcttagc ttctcttctc gtcccttaac taattaaatc agcgtaaata
    19081 ggatggacga tcttttctaa atcggctact tttatccgaa ggcttctccc gatttcaccg
    19141 cttgacacaa ttacttcttc tttttctttt aaagaactgt ctgctaaaaa acaataatca
    19201 ggatgtgcga gttttattcc aataggggtg tttgctccgt ggggatatcc tgtatgcatt
    19261 aatacaaaat ccattccagg aaaaccaact ttacgatctt tcgtcacttg tctcgttttt
    19321 ttgtaatcta agtgatcgtt caaaggaatc aatgcgatga tcaccccagt tttgtttcct
    19381 ttcactacga gagttttaca tatcaaagct ggatcaatcc ctcttttttg tagttcttct
    19441 gtgaaagatt cttctaccac atgactaaaa tcaagtggtt gatagctgat cttttgttct
    19501 tctaaataac gttcgacttt tgtttgactc atcattgatt cactcccttt attctatcgt
    19561 ttattataaa gcgagggatg ctgtattcaa aaattatttg tttttcgcaa aaaaagagca
    19621 agacgagcac tctatagaga ggttcatctt actctttttg tttatttttt atttcgacgt
    19681 tgagtagctt ccataatgac tggcaagatc actgggcggc gttttgtttg ttcaaataag
    19741 taacggccta attgatctcg gatgtcttgc tttaatttgc tccactcaaa atcattgctt
    19801 tctaaatgtt tctcaacgat tgtttcaatc atattgctgc tttctttcat cagatcacgg
    19861 ctagctttta cgtaaacgaa tccccgtgaa gtaatttgag gttttgaaat gattttcttt
    19921 tctcgccgac tgatagtgac aaccgctacg aaaatcccgt cttctgacaa gatttttcga
    19981 tcacgtaaaa caatgtttcc gatatcacct atgcctaatc catcaatcat tacattttct
    20041 gcacttgtag ttcctgcaac actcatacgc ttgttcttat attccagcac gtctccgcgt
    20101 ccagtaatat agatattttt gtatggcatc cctactgcat gagccaaatc tgcatgagca
    20161 gctaattcac gatactctcc ttgaactggc acaaaatatg ttggtttcat caaattgatc
    20221 attaattgca agtcattagg attggcatgg ccagaaacgc gcatattctc agagatcaac
    20281 ttcactgttc caccagctcg ataaatgata tcttccgttt tcgccacgat ggtttccatc
    20341 gcgatgcttg gcgtagtagt gatataaacc aaatctcctt cttcaatctt gacattacgg
    20401 tgtgtaccgt ttgccatttt ttgcagagac ttgatgggtt cacccattcg gccagtttct
    20461 aaaatgatca gttcttctgg atcatagttt ttcatgtctt tcaacgtaac aagcagatct
    20521 tcacttggta attgaagttt ttctaaacgc atcgctgtgc gaataattcg ttcgaaatct
    20581 tgaccagtca agacaacttt tcgttgtgct ttttctgctg catccaatac ttgttgcact
    20641 cgttgcaaat tacttgctac acaagcgacg atgatacgcc cttcccaata tttgatcgta
    20701 tcaaatactt catctgctat ttgggcttca gaagctacag gctgaggatt ttctgcattt
    20761 gccgattcac ttagtaaggc taagactcct tctttgccga tttccgccaa tcgtgcataa
    20821 tctgtttgat acattgggat agccgtttgg tcaaatttaa aatctcccgt ataaacgata
    20881 ctaccttctt ttgttttcag attgatcccc actgaatctg gtatcgtatg agtggtacgg
    20941 aaaaagctca ctactgtttc accaaagtct atttctgtat gctcatctac cacatggaaa
    21001 tccttaaatc ctttggctgc ttcattacga ctgacattca attttgctaa ctcgatcgtc
    21061 aactctgtcc cgaatactgg tacagaaatt tttgacaaca agtaaggaag tgccccgatc
    21121 gcatctgcat gtccatgtgt taaaaagaca ccagctacac gatcaatgtt ttcttctaaa
    21181 tacgtaaaat cagggattac gacgtcgatc cccaacaact cattttcagg atattttagc
    21241 ccacaatcta aaataaaaat atcctcgccc acttctgcga catacatgtt ttttccattt
    21301 tcacgaacgc cgcctaatgg gataatttta atggtactca caaacttcac ctcttcttat
    21361 gacatccaag tttaaagatg tcgattttct tctttatcta aatatatgag ccattccaat
    21421 gaagcatatg caaaatcact atttctcaac ttatggagaa tcagcaggaa aattaccagc
    21481 acatccgcgt tctgacagtt ggatgggcat tttattttgg atatacttca ttaacaaaat
    21541 ggatcttttg cgaaacgcta tagccagaat acttatataa gtattcagct gttccccatt
    21601 attatacaac aaataaacta aatcgctagt atcagcaatt gttagaacaa ctatgcacgt
    21661 aaaaatccct ctgtagcatc taggatttct tttgctattc ctctttcatt tcttcttcgt
    21721 cggtttgaaa catacttttt tcattttacc acattttttg caataactat agtgactttt
    21781 tatatataca gaacctcatg ttgatggtgt gcaaacagca taagatttaa gctagcttta
    21841 agataggttg tctattatca aatgcatcta ctaggaggga tgcaaaatat gaattttatg
    21901 aaacgtgcat ggaaaagtac aaaagtaaaa tggggacgtt cattgttatt gtttgccgtg
    21961 ttcacagcca ttcttatttt tgtgctagct ggcctcacga ttcgaagcgc tgcagaagaa
    22021 gcagcgaatc aagcacaaaa agaagttgga gcaacagtga ctttaagtgc caatcgagaa
    22081 gcttcattta aaaaacagga acaaacttct tcaagcggtt ctaccactga agaacgtcca
    22141 gatccaggca gtttttctct tacaccagtt tccttaacgg acgcggaaaa aatagctgaa
    22201 ttagataatg taaagagtta ttcctttgaa agttccgcct cagctatggc aaaagatgga
    22261 ataactgcca tcactagttc tgattcaagt gaagacagtt cagaaactac tgatgcaggc
    22321 ggtgaacctg ctggaggaat gggcgggaat ccgcaaatga ctcaagcaga ctttcaaatc
    22381 agtggtgtgt cagcaactgc acaaaatagt aatttttctg atggaacagc aaaaatcacg
    22441 gatggtgaag gaatagatga cagcgacaaa ggcacaaaca atgtagtcat tgattctact
    22501 ctagcagaaa caaatgacct ggcagtcgga gatactttca caatcacgag tactgaagat
    22561 gaagatacca cttatgaaat gacgatcaaa ggaatttatg aatccagcga aacaagcggc
    22621 agtatgggta tgaaattcaa cttcatgaat ccgtcgaata cactttacac ttcttacact
    22681 tttgcaaatg aactaaatgg gacatctgac gataacacga tagattctgc tatttacact
    22741 ctatctgatc cagataaaat ggatgagttt ctcaaagaag ctgagaagct gatcgacaca
    22801 gatacattta gcttacaatc aaatgattcc atgtatcagt caatgttgga acctttaaac
    22861 aacgtggcaa gtttttctaa aaatgttgtt ttactagtgg ctgtagccgg tattatcata
    22921 cttactctga tcattatgat taccattcgg gagcgtcggc atgagttagg cgtcttgcta
    22981 tctttaggag aatccagaag taaaatcatt ctccaattgt ttaccgaagt ggctatttgt
    23041 atgattcttg cattaggagt tgcttcgctt agcggaaacg tcgtcgcgaa tgccgtggga
    23101 cagcagctac ttgaccaaca gacagaaacc acgacacaaa accagcaagc tcctggcggt
    23161 ggacaaatgc caggtaacag acaagaacaa ggcggtcaac gcgatggtca acaaggaacc
    23221 acgaataatc catttgctgt ttctgatcaa gtcagcgaat tggaaatcaa tgtcaaacct
    23281 gcacaattgg gactattagc cggagtagca tttggaatca gtctgttttc tgtcttttta
    23341 gcttctatag ggatattaag attaaaccca agaaaaatcc tattaaacta aaggaggaat
    23401 ttgatgttac ggacagaaca attgggattt tggtatcaag atccgcaaga tgcgctttat
    23461 aaagatgtag aactgaattt cgaagctgga aaaatgtacg ctattttagg agcaagtggt
    23521 tctggaaaaa cgacttttct ttcgcttatt tctggactag atgcaccaaa agaaggaaag
    23581 gtatactatc aagatcgttc actgaacaaa atcgggttaa gaagttatcg taaaaatgat
    23641 gtctcgatta ttttccaagc ttacaacttg cttccttatt tatcggctct agacaacgtc
    23701 atagcagcaa tggaaatcac taagaccaag caagagaatt atcgacaagt tgctttagat
    23761 cgactggctc gtgtaggaat tgatgaaata ttagcaaaaa aacaggtgac caaactttcg
    23821 ggaggtcagc agcagcgagt cgctatcgtt cgtgctatct gctgtgaaca tcaactaatc
    23881 gtagcggatg agcctactgg aaatctagat gaaacaactt cacaagatat cgtcaaactt
    23941 ttccaagaaa tcgcccatga acaaaatcgc tgtattatca tcgtgactca tgagattgaa
    24001 gtagcaaaaa gctgtgatga agtctttgaa ctaaaaaata aaaaatttca aaaaatcgaa
    24061 gtttaaaaaa caggataatg atc
//
LOCUS       DAZULG010000033        24035 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000033 DAZULG010000000
VERSION     DAZULG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.033"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACG9CY_001178"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACG9CY_001178"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS4937702.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACG9CY_001179"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACG9CY_001179"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS4937703.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACG9CY_001180"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACG9CY_001180"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS4937704.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACG9CY_001181"
     CDS             complement(3373..4680)
                     /locus_tag="ACG9CY_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS4937705.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACG9CY_001182"
     CDS             complement(4683..5264)
                     /locus_tag="ACG9CY_001182"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS4937706.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACG9CY_001183"
     CDS             complement(5836..7128)
                     /locus_tag="ACG9CY_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4937707.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACG9CY_001184"
     CDS             complement(7125..7748)
                     /locus_tag="ACG9CY_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937708.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACG9CY_001185"
     CDS             complement(7751..8587)
                     /locus_tag="ACG9CY_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4937709.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACG9CY_001186"
     CDS             complement(8604..9491)
                     /locus_tag="ACG9CY_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4937710.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACG9CY_001187"
     CDS             complement(9507..10790)
                     /locus_tag="ACG9CY_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4937711.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACG9CY_001188"
     CDS             complement(11473..12156)
                     /locus_tag="ACG9CY_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4937712.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACG9CY_001189"
     CDS             complement(12174..12791)
                     /locus_tag="ACG9CY_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4937713.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACG9CY_001190"
     CDS             complement(12801..13625)
                     /locus_tag="ACG9CY_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS4937714.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACG9CY_001191"
     CDS             complement(13618..14178)
                     /locus_tag="ACG9CY_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937715.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACG9CY_001192"
     CDS             14352..15308
                     /locus_tag="ACG9CY_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4937716.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACG9CY_001193"
     CDS             complement(15613..16569)
                     /locus_tag="ACG9CY_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS4937717.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACG9CY_001194"
     CDS             16673..17275
                     /locus_tag="ACG9CY_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4937718.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACG9CY_001195"
     CDS             complement(17363..18862)
                     /locus_tag="ACG9CY_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS4937719.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACG9CY_001196"
     CDS             complement(18878..19402)
                     /locus_tag="ACG9CY_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS4937720.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACG9CY_001197"
     CDS             19700..20449
                     /locus_tag="ACG9CY_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4937721.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACG9CY_001198"
     CDS             20440..21225
                     /locus_tag="ACG9CY_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4937722.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACG9CY_001199"
     CDS             21194..21910
                     /locus_tag="ACG9CY_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4937723.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACG9CY_001200"
     CDS             complement(22012..23031)
                     /locus_tag="ACG9CY_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS4937724.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..>24035)
                     /locus_tag="ACG9CY_001201"
     CDS             complement(23050..>24035)
                     /locus_tag="ACG9CY_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS4937725.1"
                     /translation="IILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRAFMQDIN
                     TELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFFGTPIVS
                     AIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIATFAGLFI
                     WLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLAIGATLG
                     EVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIVIMMATP
                     AATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7108 a         5191 c         4199 g         7537 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataa
//
LOCUS       DAZULG010000034        23751 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000034 DAZULG010000000
VERSION     DAZULG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.034"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..671)
                     /locus_tag="ACG9CY_001202"
     CDS             complement(60..671)
                     /locus_tag="ACG9CY_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4937726.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(794..919)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1001..1717)
                     /locus_tag="ACG9CY_001203"
     CDS             complement(1001..1717)
                     /locus_tag="ACG9CY_001203"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4937727.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1723..2292)
                     /gene="scpB"
                     /locus_tag="ACG9CY_001204"
     CDS             complement(1723..2292)
                     /gene="scpB"
                     /locus_tag="ACG9CY_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS4937728.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2276..3046)
                     /locus_tag="ACG9CY_001205"
     CDS             complement(2276..3046)
                     /locus_tag="ACG9CY_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS4937729.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3043..3414)
                     /locus_tag="ACG9CY_001206"
     CDS             complement(3043..3414)
                     /locus_tag="ACG9CY_001206"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4937730.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3433..4320)
                     /gene="xerD"
                     /locus_tag="ACG9CY_001207"
     CDS             complement(3433..4320)
                     /gene="xerD"
                     /locus_tag="ACG9CY_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS4937731.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4416..4889)
                     /locus_tag="ACG9CY_001208"
     CDS             complement(4416..4889)
                     /locus_tag="ACG9CY_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4937732.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(4994..5857)
                     /locus_tag="ACG9CY_001209"
     CDS             complement(4994..5857)
                     /locus_tag="ACG9CY_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS4937733.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6053..8152)
                     /locus_tag="ACG9CY_001210"
     CDS             complement(6053..8152)
                     /locus_tag="ACG9CY_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS4937734.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8372..9481)
                     /gene="rpoD"
                     /locus_tag="ACG9CY_001211"
     CDS             complement(8372..9481)
                     /gene="rpoD"
                     /locus_tag="ACG9CY_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS4937735.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9505..11346)
                     /gene="dnaG"
                     /locus_tag="ACG9CY_001212"
     CDS             complement(9505..11346)
                     /gene="dnaG"
                     /locus_tag="ACG9CY_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS4937736.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11528..13843)
                     /locus_tag="ACG9CY_001213"
     CDS             complement(11528..13843)
                     /locus_tag="ACG9CY_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4937737.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14028..14888)
                     /locus_tag="ACG9CY_001214"
     CDS             complement(14028..14888)
                     /locus_tag="ACG9CY_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS4937738.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14947..15579)
                     /locus_tag="ACG9CY_001215"
                     /pseudo
     CDS             complement(14947..15579)
                     /locus_tag="ACG9CY_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            15746..16507
                     /locus_tag="ACG9CY_001216"
     CDS             15746..16507
                     /locus_tag="ACG9CY_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4937739.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16533..19124
                     /gene="mprF"
                     /locus_tag="ACG9CY_001217"
     CDS             16533..19124
                     /gene="mprF"
                     /locus_tag="ACG9CY_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4937740.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19312..20499)
                     /gene="tuf"
                     /locus_tag="ACG9CY_001218"
     CDS             complement(19312..20499)
                     /gene="tuf"
                     /locus_tag="ACG9CY_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4937741.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20867..21223
                     /locus_tag="ACG9CY_001219"
     CDS             20867..21223
                     /locus_tag="ACG9CY_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4937742.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(21464..22468)
                     /locus_tag="ACG9CY_001220"
     CDS             complement(21464..22468)
                     /locus_tag="ACG9CY_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS4937743.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            22634..23485
                     /locus_tag="ACG9CY_001221"
     CDS             22634..23485
                     /locus_tag="ACG9CY_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4937744.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            23502..23747
                     /locus_tag="ACG9CY_001222"
     CDS             23502..23747
                     /locus_tag="ACG9CY_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937745.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         6588 a         4961 c         4154 g         8048 t
ORIGIN      
        1 catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct ttttgatatt
       61 tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa ccatggaaga
      121 agttgcgcat gcagcactaa aaagacagca ctcacgatga atcctttgat caagttgaaa
      181 ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg tgttgcgttc
      241 agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat actcataaaa
      301 atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc tttatgctgt
      361 ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga taagaaacta
      421 gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg taatactgaa
      481 cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct gatcaatacc
      541 gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg aaaagcaaaa
      601 tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt ctgtacacgg
      661 gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt ttgacgtttc
      721 tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat ttgatcaagc
      781 gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg gaatttcacc
      841 gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg ggaatttcac
      901 cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat acatatctat
      961 tcaaaaaacg caacatctaa cacttataaa aagtaagtgt tcattttgag tggttcagca
     1021 aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg ccttgtaaag
     1081 tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact gcttgaagca
     1141 tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata ctgtggtttg
     1201 tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt ccttcgatct
     1261 ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt acctttgcaa
     1321 cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa tctccatcat
     1381 ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga tagatacgtt
     1441 cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct gcaacagcag
     1501 aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga taaattggga
     1561 cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc cttacaactt
     1621 gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga cgagaagtga
     1681 tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc ctttattcat
     1741 tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca tttcttgtgt
     1801 atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc cagttgttcc
     1861 atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca gtttatgggc
     1921 agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga tttcagctct
     1981 tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt gagataatgt
     2041 gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt tagtcgttaa
     2101 tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt gagtgtaaga
     2161 ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta acagtccgct
     2221 taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt caagttcact
     2281 taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat tcatagtttt
     2341 ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc ataaaagtag
     2401 tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag ccatgcgcat
     2461 ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg tcatcgggcg
     2521 tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc tgcatggcac
     2581 ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca tccgctaacc
     2641 catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct ttttctgtca
     2701 gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc aattcttctc
     2761 gaggatcttc ttcccataat tcttcatcgt ctgtgacgaa ttcttggaca ggaagcaatg
     2821 tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct gcaacagcta
     2881 gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc gtagcgatcg
     2941 gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga tcgagaggac
     3001 cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt tcttcttttt
     3061 ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga tggagacatc
     3121 cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac cgcgataaga
     3181 cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt aaataccaat
     3241 aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat aatcgtcttc
     3301 agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt tcttatggaa
     3361 actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta acatcttctc
     3421 accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca ttcttttctt
     3481 cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca attcctgtac
     3541 tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc gcagcgtatg
     3601 tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat ttttccagat
     3661 tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt cagatgcgtc
     3721 ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga tcgcataatc
     3781 acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca gtccaagaga
     3841 aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc cagtagcata
     3901 catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat caggtgcttc
     3961 aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg ctttcttcgg
     4021 cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta aaaactgatg
     4081 gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt tttcttcatg
     4141 caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg aagcaatttt
     4201 ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt gtcgtgtatt
     4261 ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt gctctttcat
     4321 aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga tagtcttttt
     4381 ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt tcttttttct
     4441 ttttcatagc attcctgaca gattccatgg aacgttagcc gatgatcttt tactaaaaaa
     4501 tgataacgtt cttctactac ttgctccact tcaagtaaaa gatcctcttc tacttcctcg
     4561 atcgtgccgc agttcaagca tagtaaatga tgatggaaat gtttggcgcc ttcttttctc
     4621 aaatcataac gggctaatcc atcattaaaa cttactttat cgacaatttt caattctgtc
     4681 aagatttcaa gggttcgata aacagtagca agtccaattt ccggactctt ttttttcact
     4741 aaaaagaaga tttcttctgc agataaatgc tctttctcat tttccaaaag cacaagcaga
     4801 gtagcttctc tttgcggtgt cagcttaaac cccgcttcat gtagctgctg ttttgttttt
     4861 ttcatcgccg aaatagcatt catgaccatt tactcaaccc caacaattta taataattac
     4921 aaataaaaaa agatataaca ctatgttaat taaaattatt ataagctagt taagaaaaac
     4981 atgcaaggga ttctcaatta gattttttga ctagttttgt cacaccgttt tcttgtgtaa
     5041 tcaatccttg tttcaaaaga ttacctaatg cgcgcttaaa tgctcctttg ctgataccaa
     5101 aagctttcat gatttcttct ggatccgatt tatctgtaaa gttgatttgc tgatctgcag
     5161 aacgttctaa aatggttaaa atcatttgtg catcatctga tatagcttca tatgctcttg
     5221 gcttcaatga aagattcagt acaccgtctg gtcggacacc aatcactcga ccttttatct
     5281 gctcgcctag acgaggttca ttatatcgtt cagaaggatg gataaaccca agatagaaat
     5341 cttccgttaa aacgtacgta cctgttaatt tcaagcggta tgctgttcct atcacatctt
     5401 tgtttttcat ttcttctgtt ccatatttgc ttaaagattt gaatattttt tcatctgcta
     5461 aactgcccca aatacggtct tttgcatcaa ctctaaggga aatcatcaag cgatcccctt
     5521 ttttaggcca tagctctgtc attgtcggca attcatcaag agatacgaca aagtctttat
     5581 ctggaagacc gatatcgaca aacgcaccta aatcttttcg tgtagcagta acgattccaa
     5641 aagcgtaatg accgatacgg cttttaggaa tttgtgtagt gatccgattt tcatgttttt
     5701 gattttggta accgaatcct tcaactgctt caccgatttt gtgttcacct tctgatttgc
     5761 ttaagccaaa ggtttgtccg tttttttgga caaagtagtt tttttcattt tcatcaataa
     5821 taatacctgt aaatatagtt gctaataaat cgttcatttt tttctccctc tatagtgaaa
     5881 ggttttctta atacatttct ttgctatcat aacataatgt tatctacttg aatagctgag
     5941 ccttggtttg gaaaggtgaa aaaaggagta tgacaacagt tgtgtcatac tccccttttt
     6001 cgaataaacg gtgttaaacg gcaggctcac agcctctttc atttgcctaa aattaattgc
     6061 tcccatttcc tccgaaccat ccagttttac agttgatggt tacgatgtaa aatggcaaag
     6121 cactttctcc aatgcctttc atcaatgcag attttgttga agtggcatat agattataat
     6181 agccttcccc attttcgatt ctggcttttt ctaatctgta acctccttca acgatatatc
     6241 cacgttggat acatgtagca atcactttgc tttggacacc tggtgcccac gtccatgctg
     6301 gattagatgc atcggcaata tcacttgctc tggcagattt gattggctga ttcgctgagt
     6361 ttgaagagga aggggtcgag gatgccgctt cttgctgtgc tgcttgtgcg accgcagctt
     6421 gttgagcggc ttgagctgca gctgctgctt gagcggcttg ttttgcttgt tcttcagctg
     6481 cttttttcgc ttcttctgct tttttctttt cctcttcttc ttttttctct ttatcagtga
     6541 gcatcttatc tacttgtttc aattgttcat tcaactgagc agatagatct gtatttttca
     6601 ctttttctac tgcttctttt gccgtcttgt attggtcgcg tgttgctgaa tccactactt
     6661 ttccgtcttt aaagactata gctgtttttt ctttagctgt ttgcaattca gcatattgat
     6721 ttttagcttc tgtttgtgct tgttgaatca attttccaaa atctgtgtta tccgtttcga
     6781 ttgcttggat ctctttatct gctttcaact tgatgtcatc tgctagatga tcatcgacga
     6841 tggcaggtgc tatgaaccac tcattcactt tttggatatc tcggatctta gattggatat
     6901 cttggtaagt tgtttctaac tctgaatatc ctttttcatt cttaagactg gataaatcat
     6961 tttttagttt tgataaatct tgatgaatca tatctgcacg aataaattga tggtgatcat
     7021 ctgtataaaa ggcagcaagt ttctcatgaa gttcttccat ttgttgctgt ctttttgctt
     7081 ctgctgctac ttcggcttct actttttgat gatggtcata ataagcccag cttcctccac
     7141 cgagtagaag tactgctgct aaaacgagat aaggtacttt ttttgatttt ttcttttgcg
     7201 ctttatctgg atctgttttt tctaagtttg attcttgatg tgttttttca gtatctggca
     7261 cgattgttat tgatttcgct tctgcttcaa acagcttagg ctggttctct tctttttcta
     7321 tttttgcttc aagatcagta ccctctttgc ttacagattg attttcagca tcggattcac
     7381 tatttatttc atcgggctct gttgactttt tgtctgtgtt ttcgagtatt tcttttttct
     7441 ctgcttccgg cagtttattc gttttttcct tttctaattg tttttcagta aatacttcag
     7501 ttttttctgt gatatccgtt tttacgtctt ttatttttac tgtttcttta cctgtggcgt
     7561 tttctctatc agagtctttt tcagtttctt gttccttttc tggctctact ttgttttcct
     7621 gtgcaatttt ttcactgatc attttgagca gctctttcgc gtgctcttct tttgtatctt
     7681 cacgatgtgc tttgatataa gcggatagta tcgaattttc ttcctcatta tcgacgattt
     7741 ctttcagttc agctactctt cttttttgtt ttgattcaag cgtttcttct tgattctttc
     7801 ctgattcttc cgaagtctca tgaacagttg ctttcttctg ttcatttttc tctatattag
     7861 ttacttgatc gtgagcaggt ggttcttcgt cttctggttc tgctttttcg aataattcca
     7921 ttacagattc tagaggtaaa tctttcaatt ccgaccattc gattggatcg tttaattctg
     7981 tatcaggaaa gactgcatct tcagttgatt tatcctgcat agctgcatct gtatttgttt
     8041 cttcaattga ttcttttatt tgctgatctt ctgcagttat ttgatgaccg cagtctggac
     8101 agatagtatt ctgctccatt tttttcgatc cgcaatttgg acattttttc atcacttagc
     8161 gatcggctcc tttactaggt ttacgctacc atttaatcta tcatattcta tacgtcacgc
     8221 caatagctta tttttaggat ttacttaaaa ataatgaatg aatttccttc ctatagaaaa
     8281 aaaagcttga gactgcgtag tctcaagctt tttttctttt aaaataaacc aaacagtttt
     8341 actgatttct cttctggtca atagtccacg cttattctaa gaaatctttt aattgttttg
     8401 aacgagaagg atggcgcaat tttcttaatg ctttagcctc gatctgacga atacgttccc
     8461 gagtaacacc aaatactttc cctacttctt ccaatgttct cgtccggcca tcatctaaac
     8521 caaaacgtaa gcgaaggaca ttctcttccc gatcagtcaa tgtatctagt acatcttcca
     8581 attgttcttt tagtaactca taagctgcgt gttctgctgg acttgttgct tcctggtctt
     8641 ctatgaaatc acctaagtga gagtcatctt cttcaccgat tggcgtttca agggaaacag
     8701 gttcttgcgc gattttcaag atttctcgga ctttttctgt tggaagatcc atttccgcac
     8761 cgatttcttc tggcgtaggt tctcttccaa ggtcttgcag caattgacgt tggatacgta
     8821 tcaatttatt aattgtttca accatatgaa ctggaatacg gatcgtacgt gcttggtcag
     8881 cgattgctcg agtgatggct tgacgaatcc accatgtagc ataggttgaa aacttaaatc
     8941 ctttgcggta gtcaaatttt tcaactgcct tcatcagtcc catgtttcct tcttggatca
     9001 agtcaaggaa ctgcatgcct cgtcctacat aacgtttagc gatacttact actaaacgaa
     9061 ggtttgcttc tgccagacgc tgtttcgctt cttggtcgcc ttcttcgatt ttcaatgcta
     9121 acgctacttc ttcttccgca gttaaaaggg agacacggcc aatttctttc agatacatac
     9181 gaaccgggtc attgattttt acaccggttg gcgcagaaag atcttctgtt tttgcttgtt
     9241 cagctttttt ctcagcactc ttcaagctgt gaattgatgg atcaccattt tcatccacca
     9301 cactgatacc tgcgtcttca atcttttgga tcaatttgtc catttcatct gcattcaaag
     9361 tgaatggtgt agcaagctga ttggataaat catcatatac aacttgtcgt ttgggtttgt
     9421 tttctttgat gaaagctgct actgctgctt catacgtttt tttatctgtt ccatttgcca
     9481 taaaagaagg ccccccttga gtagctattt ggcttgtttt aactgtttcg ttaaattgat
     9541 gatttcaatc gtcaattcaa gctcaagctg tttgtttcca gttcgtctgg cttcttgctg
     9601 catttgtttt tttaatgtta tttcatcagc taagccagat ttgctgatca catctagcaa
     9661 gtcttggatt tctcgtggcg aactttcttc tgccaccggt aaatattcga tttccaccac
     9721 caaccgcctt aaattttcat cttgcaaaaa gtctaaaaac tctgcaaggt tgaattcata
     9781 atgttgtgag acatatgcat cgaataaaaa ataaacttct gcatatgcat catgaataaa
     9841 ctgaaattct cgttctttta aaatattacg gacagaaact tctttaaatg cacgatataa
     9901 tagaatctgc tctgcctttt ctacttgcgt caaaggtctc tttttccgtg cagtttcttg
     9961 tctcgtaacc tgagtgtgtt gactttcctg aggttcttga cgattttgct gatttgtcct
    10021 tcgaagatcg cgcagttgtt tttgtatcgt ctctctgccg atctgaaact ctgttgacag
    10081 ttgagataga tacatatctt gttcgattgg tgaaacaact cggctaagtt caactagtaa
    10141 ttcttcgagg tattcgatct tgtctttttc attatctaaa tttttccctt gttttaaata
    10201 acgtgattta aaagtaaaga ctgtttcgcg cccatgagcc actaattctt ggaaagatgc
    10261 ttctccatac ttacggagat attcatcagg atcaagcttt tctggaacga ctacgacaga
    10321 aagtgctaat cgactgtttt gccgaagcaa ttcaatccct cgattggttg cttcaaaacc
    10381 tgcgttgtct ccgtcatagc aaaagaccaa ttctttcgtc agcttttcca tttgttggat
    10441 ctgttgagct gttagacttg tccccataga agctaatcca gttttgatac cagcctgcca
    10501 tgcagcaatc acatccataa acccttcgaa caacaaaatt tctccgtttt tccggatatc
    10561 gcttcttgct ttatcaagat taaataaaac ttgtctttta ttgaacagtt ctgtttctgg
    10621 actattcaag tatttgggct gatctttttc ctgccctttt tcaggtgcaa gccagcgtcc
    10681 tgaaaaaccg atcgtctggc cttgagaatt acgaagtgga aacatcactc tttgataaaa
    10741 acgatcagac atacggccat catcatgcgt gacaaacaat ccagagcgtt ctaacaattc
    10801 atttgaaatt gattcattct tgaatacttg ctgaagaaaa gaacgctcat taggtgcaaa
    10861 acctatgttg aattcttcaa ttaattctaa agacaaccca cgactttgca aatattctaa
    10921 agctggctgt ccagcttttg tatgcaatag catgtgatga tagatttccg ctgctttttc
    10981 atgagcagca atcagtttcc ccgtagcaga ttcaggattt tggacagcta tagtacgctg
    11041 acgccattga tcttctaacg gtatctgttc gaagtctgcc acttttacca ctgcttctgg
    11101 aaaacttaga ccttctagtt cttgaagaaa agtaaacacg tttccgcctt taccacaacc
    11161 aaagcaatga aaaatctgct tgtcttctgc aacagaaaaa gacggtgtac gttcttcatg
    11221 aaaaggacac agccccaagt aatttttacc agactttttc aactggacat attgtccaat
    11281 cacctcaacg atattcgttt gactgcgtac tgtttcgata acctcttgag gaatccgttg
    11341 agccattttt tcaccccctg ctctattgat atcttttggg tttaacagaa gatatttcag
    11401 gagaataacc ggtaaaaaat cgcactaact gaaatcagtg cgattttaaa ggacacaatt
    11461 atacatgttt gattgtatca tatttcattg ctttttcaag tataacacat ttctatctca
    11521 ttaagcatca atttgctact cttttcagta actgattttg aatatcagcc ttccagtaca
    11581 atactcgaag aacaatattt acgagcatga atacaaagaa gtatcctacg ctctctccca
    11641 accctaagtt tacttccaaa attccatgga acagcattgc agctacatat atccctacga
    11701 tcgtcgttac tgctaggaag atgcgcaatg ggttgaaggg ataacaagct tttacaactg
    11761 ccatgcaact gatcccaatc agcaagtaat acatcaaagt agtagtttca gcatttcccc
    11821 atgcgaatgt ttttccaagt agatacaccg caatgatatt cactactaca agcaaggcat
    11881 ttggtaaagc attaataagt gctgtaggca aataacggta agtgattttt cttttatctt
    11941 gttcaaaaga taaaaagaag gaaggatacc cttcaatcgc taaatcaatc agcgtgattt
    12001 ggatagggat aaacggaaat ccaattgctg taacggcaca tataatcgat aaaataaatg
    12061 aataaatcgt tttgataaaa aagatgcctg agactttcgt cacattgttg accactcggc
    12121 gtccttcaaa caggacttct ggcaatgtag taaaatcaga gtccaacaat accagattcg
    12181 caatctgacg tgttgctcca tcaccttctg ccatagcaat gctgcaatct gcttctctta
    12241 acgccaaaac atcattcaca ccgtcgcctg tcattgcaac cgttctgccg ctttctttca
    12301 attcatttac cagcaatttc ttttgctgcg gactcactcg tccaaaaacc gtatagcgat
    12361 gagcagcttc tcgcacttcc atttcttcag taatttgaga aagatcgaca tacgcatcat
    12421 aattcggtaa tcctgcacgt ctagcgatat tggagacagt aaaaggattg tctccagaaa
    12481 tcactttcag atccacacct tcctcatgaa gataagctag tgtttcttga gcgttttttc
    12541 ggattggatc atcgatttca aacagagcaa ccggctcaac aaatgctggt ttttcttcgt
    12601 tcaattgctg atttggagcg atccccaaca taagtacgcg atagccattc gcttgggctg
    12661 tttttagact gtccggaagc cgatcttctt ccaccaaacg ttcaggcgct cctacgtaca
    12721 cgactcccag ttctggaaat tccatcgcgc cccatttgcg atcagaagaa aatgcgactt
    12781 tgtttgtcac atcataccga ttagaaagct caaaataatc tctcatagcc cgcatagtga
    12841 tgttgttatc actgctttct gccagatacg tccccataat ttctgggatg gctttctcgt
    12901 acagcggatg tagtgcttct acccgctgga tactcatttt cccttcagta atggtccctg
    12961 ttttatccaa gcataatgta tcgacatggg ccagcgtttc aattgaatac atgtcttgaa
    13021 caaggattcg tttttttgcc agtttggtta cccctgtcgt taatgcaata cttattagca
    13081 ataccaaacc ctttggaagc atgcctaaca aagctgctgc agatgcaata acagaaattt
    13141 tgatagaagc atcgcggatg aacagtgctt ccaaaaatag tatgatcccc aaaggaataa
    13201 tcacgaaact agtgaatttg gatactttgc gaatcgaatc tacaagttca gaacgaatag
    13261 ctttgtgagt ctttgcctcg ctagtcaagc ggacagcata attatctgca cctactcgga
    13321 taacttcagc catgacagaa ccgcttgtca aaaagcttcc agaaagcaat tcagctccag
    13381 tttctttgac aatcaaatca gattcgcctg tcaaaagagc ttcatttgct tctgcccgtc
    13441 cagatataac ctgtaaatcg gctggtatct gttctcctgc tccgattttt accaaatctc
    13501 ctaataccag ctctttagta gaaatcgatt gttctttacc gtcccggatc actagtactt
    13561 ctgattggga aacaatcgat aatttccgta caagatttct tgcatgtatt tcttgataaa
    13621 ttccaataac gatattgacg acaataattg ctaaatacgc catgttggaa tacgcaccta
    13681 caaataacag acaaatccca ataccaaagt tcagaaaatt gaataacgtc aatatattat
    13741 cgctgaatat ttgttttgta ctttttgatg catcttcctg gtagtcattt tgctggccag
    13801 aagccatttt ctgaactacc tcttgctgtg taagtccatt cattttcttt ttctccattt
    13861 tccgccaact ttcaaaaata atcacgtgtt taagtctagc gtgttccaag attaaaaaca
    13921 acttgttttt ataaaattta tagaaaaaca cacgaatgat tttacaaaaa agatgccaac
    13981 ctttttgttt gaatcacccg tgtgctgttt ttattatcaa tctatcttta cttatattgt
    14041 tcctccaaac ggctcatcca ccaacctagt gctgtaccgg ctataaaaag aacaggacaa
    14101 gctagtaccc cagcaactcc caatcccgcg taatcggtag gaatgatcat aatcgttgaa
    14161 gcaaaaacta cacctaaaat gaaatggaat agcttgctat atgcctttga gaaaatgtaa
    14221 tccatgattt ttgacaatcc aagaacacaa agaatcccac caattccgat cggcacaatc
    14281 acttcaagtc gtacttcttt aaatccgtca gccatcgctt tatacaatcc catatacacc
    14341 aaaaaattag aagggctcaa tcctgggacg atcataccca aaccaatcaa tccgccggca
    14401 atcagccatg ttccaaaatt ctgatcgact tgcccgtcaa ataattgagc gccgtataat
    14461 aaaaacaaat aagccagaac aaaagtaaca accatgatga tcacatcaga agtatctctt
    14521 ccttttttcc ctgcttcttt ccataacgca ggaacagttc cagcgataca tccgacaaaa
    14581 aaccataata tcgttgtttc atacgcacct aataaaaaac tgactaaaaa agaaagtaga
    14641 aagatgcctg tcactccgcc tagaccaaca ggcagaaaaa acaaaacatt ttctttgaaa
    14701 ttttttgtaa ggtgggctaa aaaagaaatc agccgctcat acatgccaaa gacagctgct
    14761 aatgcaccac cgctgactcc tggcaagata aatcctgaac cgataaacat tcccttcaca
    14821 aaccgtaaca tccagtcttt ggttgtgtac cccacttctt gattttccat tttttgcctt
    14881 ttttccacta tttgcgtact ccttctttca tttaatttta ttcatgattg aacatttcct
    14941 gctattttac ttaaagactt tttcataggt ctctattatt ttttcattca ttttgacaat
    15001 atcaggataa aacatgttac ctccattatg atagagatag ccgccattgt aaagcaagga
    15061 ctggatccgc caatatcggt attgtttccc ttcctcattt cctaaaagtg ggctcagcac
    15121 atctttagaa tacgcttctg ctaattctac tgtgttttct ccgccatgat tttttacata
    15181 ggcaatataa tctttcccaa aattatatgc ttggacagct gtccataaat cgcatccttg
    15241 tttctttgct tctactatca tttccgccaa atatttgaca ccttgatcaa tactgtcagc
    15301 tggattatca atgatctcag cttctccatg cagactttcc gagctctgca tgagatctgt
    15361 cccttttcct tttgattcgg tcaatattat cgccttgatc aaattctcgt agttttccat
    15421 atgataattt tttgcagctg ttgttatttc tatttgatag ctttccactc gttttaccga
    15481 acgataagtt cttcctcata aaaacaacac cgcaccaata accgtcagaa tgatcatgta
    15541 aaaaagaata cggataatag ataatcgttt tcgcttcatt tcatcaccta gttattttga
    15601 catctttttt tatcttacta tttctttatc ttcttggcta tttcttcctt gatttctgtg
    15661 tacgccttaa gaaaaaatga attttcctgt attttttgac atcttacgaa aatgatcgtt
    15721 gtttttcagt aaaaacaatt gcccaatgac tgatataatc gttatacttt acacagtagg
    15781 tgattatgtg aaaaaagaaa agaaatatgc aaaaatgcca gatgggagcg aaatttacta
    15841 tgaaaagagc ggtcaaggct tccctctctt tttactgcat ggcaatgacg gcagcggccg
    15901 ctttttttca gaacaggtcc ctgtactcga acgctactat accgtgtatc tagtagatag
    15961 ccgaggacat ggtcgttcga caaacgaagc atctatgttg aattttcagt taatggccga
    16021 ggatctgaat acgatcatgt tgctggaaaa aatcgatcag gcagattttc ttggatttag
    16081 tgatggagca aatctagcac tggtatttgc cagcagcttc cctaaaaaag ttcatcgatt
    16141 gattttaaat tctggaaaca cgcttgttaa aggcgttcgt ttttctgcca gagtcatcag
    16201 taatattcat tatgcatggg tatggttact ctctttattt cgcccaagtc ttcgcaaaaa
    16261 tcttttagtt atcaaacttt tgctgcatga catcgggctt acagaaaatg atttaaaaaa
    16321 aatcaactcc cccactttga tcatcgtggg aaaaaaagat gtaatcaagc taaaacattc
    16381 tttgtatata gctaaaacta taccaaaagc ttcctttgtg cttgtgaaag aacaaggaca
    16441 tgaattagct agaaaagatc ctgaacgctt caatcgagaa gttcttcaat ttctttctga
    16501 aacataacta ttaggaatag aaggtgattt atttgttaaa aaataccata caatggctga
    16561 aaaaccacct aggactgttc aaaacaatct ttttgatttc tgtcattgtc attattgtcg
    16621 gtgaattgat gtccatcggt aaaactctat ctatccagca attaggagaa acgtttgcca
    16681 ctatacctgt atggaaaagc ggactaatgc tattgattgg tcttatctct gtgttaccta
    16741 tggttggtta tgatattatt ttgaatcgga tgcttgaaca aaagcagaat cctcgttacc
    16801 tgtttgagac aagctggctg atcaatacga tcaacaatat tgccggtttc ggcgggtttg
    16861 ttagtgtggg gcttcgctct gaactatatg gacaaaagaa agagagcaga aacgtgatcc
    16921 aagcattatc gaaagttttc ttgtttttga tggctggact ttctatttac agtctgcttt
    16981 cttttttact tgtagtcttc acgccagttg ctcctttttt gaagcaatat tggatatggt
    17041 tgatcggcgg cggtctatat tttcctgtcg tctttttatt tactactttc aaaaagaaag
    17101 gattccttgg tggtacctca cttaaaactc aaggacagct attgctagtt tcattgttgg
    17161 aatggaccgg tgtcctgttt agtttccttt ctgttggtta tttaatggaa atccggattg
    17221 atctgtggca aacaattcca ttattcattg ctgcttcagt gataggaatc gtgtctatga
    17281 ttccaggaga aattgggagt ttcgatgtaa tgatgattat cgggttgtcc gctattggtg
    17341 tgccaagaga aatagtcgtt gtgtggatct tgctctatcg cctgttctac tacatcattc
    17401 cgtttttgat tggaatcgtc tttttcttca aaaatatcgg ttccactttt gatcagcgtt
    17461 attctggtat ccctaaacaa ttagcaacag aaattgcgca taaaatcgtg gttgtcttat
    17521 tatacttttc tggaatcatg ctagtacttt ctgccactat cccgcaagct tttacagagt
    17581 ttcgctggct tcatagtttg aacccattga aatttcattt tattatccaa tttcctagta
    17641 tactattggg atttctttta atcattatgg gcagagggat cgcagcacgt gtgaaacgag
    17701 catatttgcc tacaatcttc ttgattgttc tagctttact ttatgtttta ttaagtgatt
    17761 ttagtttcac accggtggtt ttcttgtcta ttctgcttct gatcattctt gcttctaaaa
    17821 acgaattatt tcgggaacaa ttgatctatt catgggagtg gcgtacgatc gatggaatca
    17881 ttattggtgc actgtccttg ctttacatcg ttattggggt atacaatctg ccggatttcc
    17941 ctcatcggcg tcaccatttt atttcctttt tcttatttcc ttctgaaaag atttggtttt
    18001 cgggattgtt ggccatcatc gctgttagtt tcatgatcgt tctatttgtt catttccttc
    18061 aaggagaaaa gaaacagatt ggcgaagcct ttaatgaaga aaaggccctg aagatcctta
    18121 caacttatgg aggaaactcc gatagtcaat tgatcttctt aaaagataag cggatgtttg
    18181 cctatgaaaa agacggtgaa ccaactgttc ttctccaatt cgcttgcttc aataataaat
    18241 gtattgtaat gggtgatcct tctggtaaga aagaagattt tccagaagca atcgaagctt
    18301 ttatcgaaga aacagatcga ctatgctatt taccagtttt ctacgaaaca agcgaagaga
    18361 ttgttatgat tttgcatgaa ttcggctacg atttcattaa gatgggggaa gaagcttatg
    18421 tagacctgaa tagttttaca acttcaggga aaaaaatgaa gggcactcgt gctgtcttga
    18481 atcggatcga acgagaagga tttacatttg atgtcttaca accaccattt tctactgagc
    18541 aaatgagtac tttcaaaaat atctctgata attggttggg ttcacgaaag gaaaaagggt
    18601 tttctttagg ctttttctct gaagattatc tacaaagagc gcctattgca gttgtcaaaa
    18661 atacccaaga agaaattgtc gcttttgcaa cgatcatgcc tacttacacg aacaataaag
    18721 taggaacgat tgatctgatg cgatatgatt ctaaaaaagc accttctggc agtatggatt
    18781 tcttgttctt gcatttgttt gcctatatga aagaagaaca tatccaatgg ttcaatttag
    18841 ggatggcccc tctttcaaat gtcggaactt ccagaaagag ttttttacag gaaaggattg
    18901 catacttggt ttacgagttt ggttctcatt tttattcttt ccatggatta agagaatata
    18961 aaagcaagta cgctacaaac tgggtctcac gttatactct atattctaga gatagttgga
    19021 ttgcatatgt tatgattgct ttgctgatta ttgataatgc gccagttgag cagacacaag
    19081 gtagaataag tggtttgaaa aaatggatac gaagaaagta ttaaaaactg cataccgata
    19141 gatgaacact ctcatgatga ataaaagaga ctgaagtaaa acgaaagttc atgcttctac
    19201 aatctcttct tatacaattg aagaacaaag agcggtccaa acagaaatcc tgtttggacc
    19261 gctcttttat tttaaacatt tttcattacc aaaatattcg agtcgcgttt cttatgcttc
    19321 gatctctgta acgatacctg aacctactgt ccgtccgcct tcacgaattg agaatgtcgt
    19381 tccgttttca acagcgattg gatggatcaa gtcaacatca atcgtcacgt tgtcaccagg
    19441 catgaccatt tctgtatctt ctggcaatgt gattgttcct gtaacatcag ttgtgcggaa
    19501 gtagaactgt ggacgatagt tgttgaagaa tggagtatga cgtccacctt cttcttttgt
    19561 caacacgtac acttctgctt tgaattttgt atgtggagta atggaacctg gtttagcaag
    19621 tacttgtcca cgttcgatat cgtcacgttg gatcccccgt aacaacacgc ctacgttatc
    19681 cccagcttcc ccgtaatcta acgttttacg gaacatttct acacctgtta caactgcttt
    19741 ttgtgtttca ggtttgatcc ctacgatctc tacctcatca ccgacacgaa cagcaccacg
    19801 atcaatacga cctgaggcaa cagttcctcg acctgtaatt gagaagacgt cttccactgg
    19861 caatagtaat ggtttatctg tatcacgttc tggtgttggg atatattcat ctactgtatc
    19921 catcaattcc ataatagcag cttcagcatc tggatcgcct tgcaatgctt ttaatgcgga
    19981 acctttgatc acaggagtgt catcgcctgg gaaaccatat tcgctcaata actcgcgaac
    20041 ttccatttct accaaatcga tcaattcttc atcatcgact aaatcaactt tgttcaagaa
    20101 aacaatcagg tattttacac caacttggcg tgacaataaa atgtgttcac gtgtttgagg
    20161 catcggaccg tcagttgcag aaacaactaa gattgcacca tccatttgtg ctgcccctgt
    20221 aatcatgttt ttcacatagt ccgcgtgtcc tggtgcatca atatgcgcgt agtggcgttt
    20281 ttctgtttca tattctacgt gtgcggtatt gatcgtaatc ccacgttcac gttcttctgg
    20341 agctgcatca atactagcat agtcttgagg atttgctagg ccttttttgc ctaatactgt
    20401 agtgatcgca gcagttaaag tagttttccc atggtcaacg tgtccaatcg taccaatgtt
    20461 aacgtgtggt ttacttctgt cataatgttc ttttgccata atttacaaac ctcctaaata
    20521 atatagatca ctgaaaaatt tcagctctca cctttttatt atagtctttt tttgactaaa
    20581 tcaaaaagaa tacgtccgac ttactttttg taagattcga actaagctta ttatacgtca
    20641 atattggtca aagtcaacta agagcactcg tctgtcttat acgcgaaaaa agtacctttt
    20701 ataaataaaa aaatgtgttt tttgagaaaa acttcaagat agtccttaac tttcttttga
    20761 agcaataaga acaaaatgag gaaaagttta aaattcagct gttgccaaca actttcaata
    20821 gacgaaatct tctatttcgt taataataag gaaagaggtg aagtacatga aaaaattaat
    20881 tggcgtactt gtcgttttag gcatatttct cggcggaagt tttgcagctt atcattattt
    20941 ttacggcggc gaagcttatt atacaaaaat cacgacgaat ggcgaaatgt cagctaccca
    21001 agcagataac ggagagaaat tcacgactta tacctacaag caagcggctt ataataaaga
    21061 tggtgaaaaa aaacaggtga cgcttcgcga agaaagagaa catccattaa agatgaatgc
    21121 gtatcttaaa ctcaaagtca atcctcgtaa aggtgtatta agctgggaag aaataaaggc
    21181 ttctgaagtg cctaaaaatg cggcagaaaa aatcgataat tagtaaatgg tcttaaaatt
    21241 ttattagata aaaagaggct gggacagaag cgtttaactc caagaaataa gaaggagttg
    21301 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    21361 tctcacgctc tttttttgtt atattcgtct tttccgatcc ttctcttgac acaattcatt
    21421 taactgatca ctggtacttg aacacctaat attctagtca tttttataaa cgattcaata
    21481 cttcatccac atttttgatc atcacggata tcgtaccatc cggattattc acaaattcaa
    21541 tcaaatctgg gttgcgatat acatctactg gtacgatcag ttcgatcccg tttgatagct
    21601 tgaatttttg ttttccaaat tttttctcag aaatttcttg tacttctttt acaggaggtg
    21661 cttctgggac aaatccttgc tcttgtactt cttctttaaa agccattttt gccgtgatgt
    21721 tttccttgaa gactttttcc gcaatcatct catgatccaa ccgacctttt tcttctatcg
    21781 tatcatagac agcttctttc aaatcagcca tgatatcaaa ttcttctgtc tcaaattttt
    21841 ttccaagatg cttggctact ttttggatca cttttacatt ttcttctaaa gaaggtgcag
    21901 gaacactttc gatcactctt ccagaaaaat aaagctcttt tttgccagaa aactcatatt
    21961 ttttttcaat taattcgtag gtccaatcag tcaaattcac agcgaatgct tcatctgctt
    22021 tttgtgtttt cgcccctaga attgctcgat tcaaaatcaa gcggttgtcg atccctgctt
    22081 cgtctgcttc aacaaagtga gtgtaagcct cacgataatt gactttcaat aaagataagt
    22141 acatcactgt atctaattca tacaagacaa caaatacatc tgcactaggt gcttcttcac
    22201 tttcttggta gatatcgaac caccgctgaa cgattgcttc acttgcttcc acaaaatttt
    22261 cttgagcgat tttaagtgat tcaacgatca tgctttcttc tgctagttgt ccagtttttg
    22321 tttgagcaga agagattttt tggatcttct tttgaagaaa atcacgaata tattcttttg
    22381 tcagatccag ctctttttgt gaaaagactg gagaaccagt ttctctgtca ataatatgca
    22441 gaattgcttt ttttaaatag atatccattt tctcattccc ttctgtctta gtttacggaa
    22501 aaaaaatcaa aaataccaga atcaaaatca ttttctttta aacaaaaatt tcaagagtag
    22561 aaacaaaatg gattcttttt ttcgaaaaat gcgctacact tatagttgaa cgaataaaaa
    22621 aggagctgaa agtatgaatt cattaacaag cacttatcgt ctatcaaatg gttacgaaat
    22681 cccggtagta ggattcggca catggcaaac acctgatggg gatgtcgctg tttcctctgt
    22741 taaagaagcg ctggctgcag gttatcgcca tatcgatact gcccaaggat acaagaatga
    22801 agaaagcgtc ggccaagcga ttaaggaaag tggaattcct agagaagaga tattcttgac
    22861 aacaaaatta tggaatgcaa accacagcta tgaacttgtg atgtcttctt ttgaagaatc
    22921 attgaagaag ctccaaacag attatttaga tctgtttttg atccactggc caaaccctgt
    22981 tgcttttcgt gataattggg aacaagcaaa tgcagataca tggcgagcat ttgaagaact
    23041 gtatgaagca ggtaaaatca aagcaatcgg tgtgagcaat ttcttacccc accatctaga
    23101 tacactagca aaaacagcaa aaattatgcc gatggtcaat caagttttcc ttgcaccagg
    23161 cgaattacag acaactgtag ttgagtatgc aaaaaaacat gatatgatcc tcgaagcata
    23221 tagtccatta ggcactggta aaatcttcga tgtgcctgaa atgaaacaaa tcgctgaagc
    23281 acatgacaag acgattgcac aagttgcttt gcgttggtcg ctgcaacacg aattccttcc
    23341 tcttcctaag tcagtgacac caagcagaat caaagaaaat actgaattat ttgattttga
    23401 attaacagaa gaagaaatga aacaaatcga tcagttagat ggtgtagtcg gcaaagcaaa
    23461 agacccagat actacccaat tttaagtcga aaggtgattt tatggatcca atcgaacgct
    23521 tgaattctct ctcggaagaa gttacccaga cttttcattc cgattttgtc tttttgatcg
    23581 atgcagagaa aatccaacat tttcctgcaa gaaactggac acatgatcaa atcatcgaag
    23641 aactgaaaaa acggttcgat cattcgctaa tggtcaaacc ctggcacgaa catgaagtca
    23701 tttattcacc tgaactacct gtttttgcct tgataccgaa aaaatagaaa g
//
LOCUS       DAZULG010000035        23654 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000035 DAZULG010000000
VERSION     DAZULG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.035"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..602)
                     /locus_tag="ACG9CY_001223"
     CDS             complement(27..602)
                     /locus_tag="ACG9CY_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS4937746.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(607..1581)
                     /locus_tag="ACG9CY_001224"
     CDS             complement(607..1581)
                     /locus_tag="ACG9CY_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFS4937747.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1605..1649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1864..2832
                     /locus_tag="ACG9CY_001225"
     CDS             1864..2832
                     /locus_tag="ACG9CY_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4937748.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2929..3222
                     /locus_tag="ACG9CY_001226"
     CDS             2929..3222
                     /locus_tag="ACG9CY_001226"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4937749.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3340..3576
                     /gene="secG"
                     /locus_tag="ACG9CY_001227"
     CDS             3340..3576
                     /gene="secG"
                     /locus_tag="ACG9CY_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS4937750.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3679..4434
                     /locus_tag="ACG9CY_001228"
     CDS             3679..4434
                     /locus_tag="ACG9CY_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4937751.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4437..6794
                     /gene="rnr"
                     /locus_tag="ACG9CY_001229"
     CDS             4437..6794
                     /gene="rnr"
                     /locus_tag="ACG9CY_001229"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS4937752.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6816..7280
                     /gene="smpB"
                     /locus_tag="ACG9CY_001230"
     CDS             6816..7280
                     /gene="smpB"
                     /locus_tag="ACG9CY_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS4937753.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7582..8301
                     /gene="atpB"
                     /locus_tag="ACG9CY_001231"
     CDS             7582..8301
                     /gene="atpB"
                     /locus_tag="ACG9CY_001231"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS4937754.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8354..8569
                     /gene="atpE"
                     /locus_tag="ACG9CY_001232"
     CDS             8354..8569
                     /gene="atpE"
                     /locus_tag="ACG9CY_001232"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS4937755.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8660..9184
                     /gene="atpF"
                     /locus_tag="ACG9CY_001233"
     CDS             8660..9184
                     /gene="atpF"
                     /locus_tag="ACG9CY_001233"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS4937756.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9171..9713
                     /locus_tag="ACG9CY_001234"
     CDS             9171..9713
                     /locus_tag="ACG9CY_001234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS4937757.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9736..11292
                     /gene="atpA"
                     /locus_tag="ACG9CY_001235"
     CDS             9736..11292
                     /gene="atpA"
                     /locus_tag="ACG9CY_001235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS4937758.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11306..12208
                     /locus_tag="ACG9CY_001236"
     CDS             11306..12208
                     /locus_tag="ACG9CY_001236"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS4937759.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12231..13637
                     /gene="atpD"
                     /locus_tag="ACG9CY_001237"
     CDS             12231..13637
                     /gene="atpD"
                     /locus_tag="ACG9CY_001237"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS4937760.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13651..14073
                     /locus_tag="ACG9CY_001238"
     CDS             13651..14073
                     /locus_tag="ACG9CY_001238"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS4937761.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14542..14772
                     /locus_tag="ACG9CY_001239"
     CDS             14542..14772
                     /locus_tag="ACG9CY_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS4937762.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14869..16170
                     /gene="murA"
                     /locus_tag="ACG9CY_001240"
     CDS             14869..16170
                     /gene="murA"
                     /locus_tag="ACG9CY_001240"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4937763.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16183..16359
                     /locus_tag="ACG9CY_001241"
     CDS             16183..16359
                     /locus_tag="ACG9CY_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4937764.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16494..16790
                     /gene="yidD"
                     /locus_tag="ACG9CY_001242"
     CDS             16494..16790
                     /gene="yidD"
                     /locus_tag="ACG9CY_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS4937765.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16854..17321
                     /locus_tag="ACG9CY_001243"
     CDS             16854..17321
                     /locus_tag="ACG9CY_001243"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4937766.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17406..18290
                     /locus_tag="ACG9CY_001244"
     CDS             17406..18290
                     /locus_tag="ACG9CY_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4937767.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18404..18640
                     /locus_tag="ACG9CY_001245"
     CDS             18404..18640
                     /locus_tag="ACG9CY_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS4937768.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18728..18808
                     /locus_tag="ACG9CY_001246"
     tRNA            18728..18808
                     /locus_tag="ACG9CY_001246"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18762..18764,aa:Tyr,seq:gta)
     gene            18815..18886
                     /locus_tag="ACG9CY_001247"
     tRNA            18815..18886
                     /locus_tag="ACG9CY_001247"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18847..18849,aa:Gln,seq:ttg)
     gene            19012..19182
                     /locus_tag="ACG9CY_001248"
     CDS             19012..19182
                     /locus_tag="ACG9CY_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937769.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19402..20109
                     /locus_tag="ACG9CY_001249"
     CDS             19402..20109
                     /locus_tag="ACG9CY_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4937770.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20168..20812)
                     /locus_tag="ACG9CY_001250"
     CDS             complement(20168..20812)
                     /locus_tag="ACG9CY_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS4937771.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20939..21226
                     /locus_tag="ACG9CY_001251"
     CDS             20939..21226
                     /locus_tag="ACG9CY_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937772.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21334..21654
                     /locus_tag="ACG9CY_001252"
     CDS             21334..21654
                     /locus_tag="ACG9CY_001252"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4937773.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21735..23048
                     /locus_tag="ACG9CY_001253"
                     /pseudo
     CDS             21735..23048
                     /locus_tag="ACG9CY_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23108..23464
                     /locus_tag="ACG9CY_001254"
     CDS             23108..23464
                     /locus_tag="ACG9CY_001254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937774.1"
                     /translation="MSKSRLPQTCSVCRAQFHLLEAKGCAGCQKPIHLKYEETKKKAN
                     TSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWINRYKFMGDIRLAGTFAYKWTQ
                     IKKRLPGIYLLSNTFI"
BASE COUNT         7820 a         4125 c         5092 g         6617 t
ORIGIN      
        1 tattttgcta atccagctct gtttattcag cctatttttc tttttgaata tatcaagcgt
       61 tgtggcaaca tagtgatgac taatgaaaca acgacgatag acaacaaacg atcaaggtag
      121 tctgtacctg cctgtaccaa gatcacactt gttgtctgac ttaaacctaa cccatgtaat
      181 agctgaacga tcatactgga tcctgaggag gtgatcccgc caaataactg aacggtaatg
      241 atcgaagaaa cgatcgttcc aggtaccgac aaaatcaaag cgagtaataa cagtttcaag
      301 ctgtttttat ctttgccgag atatcttggc aaaatcaatc cagcaagcaa gcctgtgaga
      361 atttggattg gcgaataata cagagaaaaa atatctgtag tcactccact taaaataccg
      421 ctggacaatc ctgtcagcat tccaggtaca ggcccaagca atgcaccagc aaaaatcgtt
      481 ccgatagaat cgagatagat tggcaaacga agccataaag caatcgttcc tccaatataa
      541 ttgatcccaa tacacaaagc catcactgta agtacgcgag ttgccatttt gtttttattc
      601 attttgttag cccatcttta acctttccaa atctttcata atcgtttctt cttgtgcatt
      661 taaaagaacg gttaaaaact ttcggaaaaa aagacgagta tctacttttg tcataatttt
      721 actgttagca ggtttctgcc aaaattcata acgatcaacc aatgtttgtc cacgacttat
      781 tccttgagtt tccacagcag tgaagctttt gaaaccttca cagatggcct cctcgataaa
      841 ataagcaaca gctaacggat cattgatgac acatcctaaa attctttctt gtttccagtg
      901 gaagtcaaaa taaaaacgag tgatcgcttt tagatactct ccctcttcag gattcatttg
      961 acagcagtat tcgaggatag tcggtgtcaa gacgatttct cttgtcacat ctaacccaac
     1021 catttctatc gtttgtttga gattctcgaa tacataagaa gctgcttcag gatcacacca
     1081 gtaattgtat tcagcaactg gcgaacagtt accatggctt ttgtaagttc cacccatcga
     1141 gacaaaacgt tccatatgtt tcccaatatt tgggttgact ttcaaagcag aagcgatatt
     1201 ggtcaaagga cccaaagcaa tgactgatgt atttgtcttt tctttgaaat aatcagccaa
     1261 aaaatcaacg gcatggagag gttctgcctg ttttctcaag atcatcggga aatttgtttc
     1321 ccctaaacca tccataccat gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt
     1381 ttccattcct tgataaacag gaatgtctaa acgatcacag cgttccaggc atttcagcac
     1441 attctgcgta cctatatctg cagggacatt cccacaaact acggtaatag caactacttc
     1501 gatatcaggg gatttgacag ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca
     1561 atcaataatt acttttcgca ttatttccaa tctcctttct ttagagatac ttagtttttt
     1621 attctgggaa gtttccgaac cagtccaaga cgagcgtctt gattataatg cacgaaatta
     1681 gtatactggc ttttattgcg aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa
     1741 ctttaagtaa atgatacaat gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat
     1801 cctatggatc ggtacattgg ataatagata gattatagtt tggcagaaag cggcgggtgt
     1861 acaatggtta ttttttattt cattcttatt ctgataatta tagggatcag ttaccatttt
     1921 ctttcttatt tttatgcaaa gaaattagta agagcgggag tacagagagg atatgactgg
     1981 tatgaagaca ctggtcatcg tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa
     2041 cgacaaaaat tagaagaaga gtgtgaaagc ttttgggagc agggaacacc caaaacaatc
     2101 aaaagttatg acgggttgaa actagctggt cagatgttca tccatccaaa taagcagaat
     2161 aaatgggtca tttgcgtaca tgattaccga agcacaggga aaagagacat gtctcatata
     2221 ggtaaaagat atgcagagaa aggattcaac gttcttgtac ctgatttacg tgcgcacgga
     2281 gaaagtgaag gagaaatcat cggaatggga tggctagatc ggctagattt aattgcctgg
     2341 atccaactca tcttagatga acagccgaat gcgtccatta ttttacacgg cggatcaatg
     2401 ggagcttcta cgatcatgat ggcaagtggt gaaaaacttc catcagcggt caaaggattt
     2461 attttagaca gcggatatgt ttctgtttat gcagaattcc gatatatgtt aagtaaaatc
     2521 actgtctttc ctaaaaaaat ggtcatgaga tatgcgaatc attatgccca gaaatacgcc
     2581 ggttactcct taaagcaagc atcagctacg agacagcttg gcagcaatca tctgccttta
     2641 ttagtcatcc atggtgaaag agatcatttt gtccctacag aagcagcgta cacgattcaa
     2701 aatgctacgg caggggataa agcactgctg cttgttccgg aagcagaaca cttggaagca
     2761 tcgatgaaag atccgaaaac ctattggaca gtgatttttt catttattga acaaagaata
     2821 acgatgaatt aattttctct tcttttttgt aagtaattcg aggataaata attgtttata
     2881 gatcaggaga ctgctaaaat aaggtaggaa taaaaaaata tcagttattt gaggagtgcg
     2941 gcatatggaa atcaaagaag aaaaaatcgt cttgctttat tcaatgatga acaacaagag
     3001 atcggagaaa tgacatggtc agatgcgggc ccagacatca tgatcgttga ccatacattt
     3061 gtagatccag catatcgtgg tcaaaagtta gctgaaaagc tagtgtatac tggagtagaa
     3121 ttagcaagac gagacgggaa aaagatcatt cctttatgcc cttatgcaaa aaaagagttt
     3181 gaaaagaaac cagaatatca agacgtctta cgtcagtcat aagaataaca gctgaaagtt
     3241 ttgaactttc agctgttatt ccatttatat agagttgtca cggactttta tctatgataa
     3301 agttaaagag acgatatctt ttgggaagga ggctgcgata tgtataattt aattttggga
     3361 atcgtgatcg ttttatcgat tgttatggtc attgcgatta tgatgcagcc aagcaagcaa
     3421 aatagtgcag cgagtgcatt taccggcggt gcggatcaac tattcggcaa acaaaaagct
     3481 cgcggctttg aagctgtcat gcagcgatca actgcagtta tgggagctat ctggatgatc
     3541 ttattatttg tcctagcata tttatcttcg aagtagttga ataagcctct cgcaaaacgg
     3601 cgggcggctt tttttattgc aaagagaacg cttaagaaga agttatgaaa gaaactatgg
     3661 taaaatgagg cgaaagaggt gagacgagtg agacaattac caaaacccct ttatgcaaaa
     3721 catggaaagc gagcggtttt gctgcttcac gcttactcag gaagtccaaa cgatgttcga
     3781 atgctcgcac gatttttgga aaaagcagat tatacagttt atgcgccgct ttttaaagga
     3841 cacggaacaa tggagccata tgatatcttg caagaaaaag cagaaagctg gtgggcagac
     3901 actaaaaaag ccatccattt tttacagtct gagcagtttt ctgatattgc cgtattagga
     3961 ttatcaatgg gcggcatctt tgctgtacga gcactagaag aagaatcagt gattggcggc
     4021 ggttttttct gttcgccttt gtctcctgta aaaacaaacg tgcctgaaaa ctttgaaaaa
     4081 tacgtacgtc aagtgctgaa gacagcaggt aaatcagaga aagaaatcaa tgaaaaagca
     4141 gttgcttacc gctcgttagc tgaacaacaa ttaatggata tacaagatca ggcagcaatc
     4201 gtagaaagta ggttgtcaga tatccaacag ccgatatttt tagctcaagc tgggaaagat
     4261 gaaatgatcg accctaatgg cgtatttgaa actgcacgca agttgagccg tcagcgagtg
     4321 acacttcaat ggtatcctga aagtggtcat gtgattacag ttggtacagc aagacgcgaa
     4381 ttagaaaaag acgtcttgga atttttagaa aaattacctt ggaatgagga ataaatatga
     4441 caaaagaaac gataaaagag aaaattctct ttttcatgga aagcagtcgc aaacgcagtt
     4501 tttctatgga agaaatcgct gaaggtttag ggttccaaaa aagcgatgat tttaaattac
     4561 tagtacaaac gattgctcaa atggaaagag aacagagtat agttttcaat aaaaaaggaa
     4621 aagtgaaact tcctttaaaa ccaatcctga tcgaaggaac atttcgagca aatgaacgag
     4681 gatttggatt tgtaacgatt gatcccgaag aagatgacat ctatatccca aaagaagcga
     4741 ctggttttgc aatggatgga gacaccgtag caatcgatat catgaagcga gcaaatacag
     4801 atatggatcg cggagcagag gggaaagtag tcgagattcg agaccgggcg actactcagt
     4861 tagcagggga attcgttgct tattcagaag aagaaataca agaaacagat ctttacggcg
     4921 ttgttatccc gaaagataga aagctcaacc aatttaaagt atatgcagca gcagagggag
     4981 ttcgtccagt tgacggaagt attgtgctga tcgaactgac ccattaccct gaaaaagggt
     5041 atgcgactag tttagaaggg ataatcaagc aagtgattgg tcataagaat gaccctggca
     5101 tggatatcct ctcaattgtc gtggctaatg gtatccctac gaaatttcct gatgatgtat
     5161 tgtctgaagc agatgaagtt ccagatcaaa tcagcgaaaa ggatataact ggacgtagag
     5221 atttaagaaa tcagctcatt gttacgattg atggagaaga tgcaaaagat ctagatgatg
     5281 cagttacggt taaaaaatta gataacggta attattttct tggagtacac attgcagatg
     5341 tttcttacta tgtgacacaa ggcagtcaat tggacagaga agcatatgaa agagggacaa
     5401 gtgtatattt gactgatcga gtaattccga tgatcccgca acgtttatcg aatggtatct
     5461 gttcactgaa tccccatgtt ccaaggctga ccatgagttg cgaaatggag attgatccaa
     5521 atggacaagt agtctctcat gatattttcc caagtgtgat ccaaacaaca gaacggatga
     5581 cctacacagc tgtcaatcaa atcttagaag atcaagacga agaaacgatg caacgctatg
     5641 ctcagctcgt acctatgttc caagaaatgg gtgaattgca ccgaatcctt gaaacgatgc
     5701 gaatcaaacg tggcgcgatt tcttttgagg atcgcgaagc gaaaatcctt gttgatgctg
     5761 aaggacaccc aaatgacatc cagctaagaa gcagaggaat tggtgaacgc ttgatcgaat
     5821 ctttcatgct tgcggccaat gaaacggtag ctgaacatta taataaatta aatctgccgt
     5881 ttatttaccg aatccatgaa cagccaaaag aagaaaaaat gcagcgcttc tttgattttg
     5941 cttcagcttt gggcattctg gtccgaggaa cgaaaggaac gatcacacca aaagatttac
     6001 agcatgtgat cgaaggagta gaagacaaac cagaagctgc agttatcaat actatgttgc
     6061 taagaagcat gcaacaagca cgctattcag aagacaattt tgggcattat ggcttagcgg
     6121 cagattacta cacacacttt acatctccta ttcgacgcta tcccgatcta atcgtccatc
     6181 gtctgatccg ttcttacagt gaagataaaa gtgaaagcaa tcaaagttcg tgggctgcgg
     6241 aacttcctga aattgcagat catagttcta agatggaacg tcgtgcagta gaaacagaac
     6301 gtgaagtaga ttccatgaag aaagcagaat tcatgatgga caaagtggga gaagaattcg
     6361 atgggatcat cagctctgtt gtgaaattcg gcctgtttat cgaactgccg aatacagtag
     6421 agggattgat ccatatcaat gaattgaaac aggactactt ccattttgtt gaaaatcatt
     6481 tagctcttgt aggtgaaaga actggtctga cttttaaaat cggacagaaa gtgcgagtaa
     6541 aagtagtcaa agctgatcca gatgaacgag cagtagattt tgaacttgtt tccgcagaag
     6601 aggttacagc gttggaacgg ccaaaaacac gaaatggaaa atcaggaaaa cgcgaaggaa
     6661 aacaagcagg aaaacgccca gaaagaaatg gcaagaagaa caaacgatat agcgatagta
     6721 agcaaacgtt cggtaagaaa aaagggaaga agccatttta caaaggtgtt aaaaagaaga
     6781 aaaagaaaaa ataaaatgag ttggaggaaa cagctatgcc aaaaggcgag ggaaaattaa
     6841 ttgcacaaaa caagaaagct cgccatgatt attcgatcat cgacacgatg gaagcaggga
     6901 tggtcttgca aggaaccgag atcaagtcga tacgaaacag ccggatcaat ctaaaagatg
     6961 gatttattcg cgtccgcaac ggagaagctt tcttgcataa tgttcatatc agtccttatg
     7021 aacaaggaaa tatttttaat catgatccgt tgcgcacgag aaagttatta ttacacaaaa
     7081 aacaaatcat ccggcttgaa aatgaattga aaaatactgg aatcactgtt gttcctttaa
     7141 aagtctatat tcgtaacggc tatgccaagg tattgattgg tctggcgaaa gggaaaaaat
     7201 cttatgataa acgggaagat ttgaaacgaa aagatatcga tcgacaaatt gatcgaacat
     7261 taaaaaattt ctctagataa tttcattatc ctttttcgca tattcggtgc ttttgctttt
     7321 acttaatgat aaaatttgtt agcatttaat ctgataaaac atgattatca taggtttttt
     7381 cttagatcag gagcaccgtg tataaagaaa aattcgcttc gtttttgact gaaataaaaa
     7441 agtgtctaat tttcatgaaa atgttttgaa ttttctgttt catggtggta tgataccgtg
     7501 tggttagaaa ccgattggaa aaaaccagat acgcacatga attgatttta tgtgaatttc
     7561 ggggaaaaga ggtgaaatgg attggatgaa cgctcgctaa cgttccacat tggaccaatt
     7621 tggtttgatg gtacagtttg tataatggtg ttactgacct gtttgattgt tttcttctta
     7681 gcttactttt ttacaagaaa tcttaagttg aaaccaacag gcaaacaaaa tgcgatagaa
     7741 tgggtcattg attttagtcg aggaatcgta acagataatc tgcctagaaa agaattgagc
     7801 aatttccatt tgttggcatt tacattgttt ttatttgttt ttgttgcaaa caacatcggt
     7861 ttagtaacaa aaatcgtttt acctaacgaa acaacacttt ggaaaagccc gacagcagac
     7921 ccgtttgtta ctttaacttt ggcatttata atgatcttac ttactcactt attcggggtg
     7981 aaaaagttag gcttcaaagg atatttcgta aattcgttct tgaaacccta tagttttatg
     8041 tttccgatga aactgattga agaatttaca aacttattaa cacttgcttt acgtctttat
     8101 gggaatattt atgcggggga agttttgcta accttgattg caaaaatgat gacaagctta
     8161 ggctggttct cattaccatt agcaattcca ttagagatgg tctggatcgc gttctccttg
     8221 tttattggga gcatccaagc atttgtcttt gtcactttgt caatggttta tatgagccat
     8281 aagattgaag tagaagaata atactcggtt aaaaaacaaa ctactattta tctattttag
     8341 gaggaaaaca attatgaatt atatcgcagc agcaatcgca atcatgggag cagcaatcgg
     8401 agccggttat ggtaacggtc aagttatctc taaaacaatc gaatcaatgg ctcgccaacc
     8461 tgaaatgtct ggtcaattcc gtacaacaat gtttatcggg gtagctctag ttgaagctgt
     8521 tcctatctta ggggtagtta tcgccttgat tctagttttt gccgtttaat tgaatcagta
     8581 ttaaggcaga aacaacatcg tttgttactg cttttttaca tggaaactag aaatactcca
     8641 aagaaaggaa ggctcgccta tgctgaatca attggcaatt gcagaagttg gcaatgcgat
     8701 gttaggtaat atcatcgttg tcagcggctc atttttgata ctactggctc tcttgaagca
     8761 ctttgcttgg ggaccaatca gcgatatttt gaaaaaacgt gaagacaaga tcgccaatga
     8821 tttagattct gcagaacaat ctcgcatcaa ctcagcgaaa atggaacaag aacgcgaaca
     8881 acaattgtta gcctctcgtt ctgatgcagc tgatatcatc aaaaatgcga aagaaagtgg
     8941 agaattaagc cgccaaaata ttttgaagga tgctcaagaa gaagcagctc gtctaaaaag
     9001 caaagcccaa gctgatatca ctgtagaacg tgattcagcg ctgaactctg taaaagacga
     9061 cgttgcagaa ctctctcttc aaatcgcgga aaaaatcttg aacaaagaat tatctccaga
     9121 aatgcatgaa tcattaatca atcaatacat tgaaggtcta ggttcttcaa atgaaactag
     9181 ataaatatac agtaggaaga cgttacggga aagctttatt tgaactggcg atcgattcaa
     9241 atcaagcaga agaaatctat caagatctcg taagtttacg tcaaatctat gaacaagtac
     9301 ctggattagg gaatatgtta agtgatgttc gtctagaacc tcatgaaaaa cgagaaatca
     9361 tggacaagct tgtcagcggt tatgatggga tcgttaaaaa ctttttagaa gttgtttatg
     9421 agtataaccg aatggatgac cttttgttca tgatcgaaga atatgaacgt cgatatgatg
     9481 aacatcaagg gttactattg ggaagtgtca caacagcagt cccactttca gacgaacaac
     9541 gaatacaatt agaaaagaat gttgcaaaaa caatggacta tcaatcagtt gaattaaaac
     9601 aaatcgttga ttcttctatt attggtggcg cgattgttga agccaaccat cgagtaatcg
     9661 acggaagcat tcgcacgcaa ttagaaaaaa tgcgtaatca attaaacaga tagtacaaag
     9721 aggtaggtga atcgaatggc catcaaagca gaagaaatta gtgccttgat taaagaacaa
     9781 attgaaaatt atcaaaacgt gctttcagtc gaagaaatcg gtaccgtaac ttatgtaggt
     9841 gacgggatcg ctcgtgcaca tggtttagaa aacgcgatga gtggtgagtt gcttgaattt
     9901 tcaaacggct catacggaat ggcgcaaaac ttagagtcaa atgatgtagg tatcattatc
     9961 cttggcgatt tcgagtccat tcgtgaagga gataaagtaa aacgaacagg taagatcatg
    10021 gaagttccag ttggagaggc cttgatcggt cgggtagtca atccgctagg tcaaccaatc
    10081 gacggactag gtgaaatcgt tactgacaaa gctcgtccag tagaagcgat ggcaccaggt
    10141 gttatgcaac gtaaatctgt taacgaacca atgcaaacag ggctaaaagc gatcgacgcc
    10201 cttgtgccaa tcggacgcgg acaacgtgaa ttagtcatcg gtgaccgtaa aacagggaaa
    10261 acttctattg cgatcgatac gatcatcaac caaaaaggtc aagatatgat ctgtatctat
    10321 gtagcaatcg gacaaaaaga ttctacagtt cgtacacaag ttgaaacatt gaaaaaatat
    10381 ggcgcaatgg attacacaat cgttgtgaat gccggtgcgt ctcaaccagc accattgctt
    10441 tatatcgcac catatgctgg tactgcaatg ggtgaagaat tcatgtacaa cggtaaacat
    10501 gtattgatca tttttgatga tctttcaaaa caagctgttg cttatcgtga actttccttg
    10561 ctacttcgcc gtccaccagg tcgtgaagct tatcctgggg atgttttcta cttgcactca
    10621 cgtttattag aacgtgcagc aaaattaagt gatgatttag gtggcggttc aatgactgcc
    10681 ttaccatttg ttgaaacaca agctggagat atctctgctt atatcccaac aaacgttatt
    10741 tcgatcactg atggacaaat cttcttagaa agcgacttgt tctacgcagg tactcgtcca
    10801 gcggttgatg ccggactatc tgtatctcgt gtaggaggtt ctgcgcaaat caaagcaatg
    10861 aaaaaagttg ctgggacttt acgcctagac ttagcaagtt accgtgaatt agaagcattt
    10921 acacaattcg gttctgactt ggatgcagca acacaagcaa aactaaatcg tggacgccgt
    10981 acagtagaaa tcttgaaaca aaaattgcat gcaccgctag ctgttgaaaa acaagtagtg
    11041 attttgtatg ctttgacaca tggattctta gatagtatcc ctgtagacag tattttagac
    11101 ttcgaacatg aattattcga atatctggat accaatcatt cagatatctt tgaaacaatc
    11161 cgtacaacaa aagatcttcc agaagaagaa gcgttgaaca gtgcgattca ggaatataaa
    11221 gacatgttct tagctacaaa aggaagtaat tcctctacag aagataaatt gaaatccatt
    11281 caaaatgcat agtgaggtga gatgaatggg tgcttcatta aatgaaatca agacacgtat
    11341 cgcttcaacg aaaaaaacga gccaaatcac gcgggcaatg cagatggtgt ctgcttctaa
    11401 gttgacaaaa tctgaagctt cttcacagaa gtttcaggtt tatgcaacaa aagtaagaga
    11461 aatcgttacg catttgactg ctactcagtt aaatgatatt gcctcagaaa atcctagagg
    11521 ggatatcaat tacaacagca tgctgctcag ccgtcctgtg aagaaaactg gttatatcgt
    11581 catcactgca gatggcggtt tagtcggtgg atataacagc tcgatattga aacaaacgat
    11641 gtctatttta gaagaagacc accaatcacc cgatgattac gtgatgatcg caattggcgg
    11701 aactggtgca gacttcttta aagcacgcgg tatcaatcta gcatatgagc tgcgcaatct
    11761 atctgatcag ccaagctttg atgaagtccg caaaattgta aatatggcaa caactatgtt
    11821 ccaaaacgaa gtattcgatg aattatacgt atgttataac catcatatca attcgctaac
    11881 tagtcagttt cgtgtagaaa aaatgctgcc aatctcagac ttagatccag aagaagcaac
    11941 gactttcgaa caagaatata tttttgaacc ttcaaaagaa gaaatcttgt ctcagttatt
    12001 gcctcagtat gctgaaagtt tgatctacgg tgcgatcgtt gacgccaaaa cagcagaaca
    12061 tgctgctggt atgacagcaa tgaaaacagc aactgataat gctgctaata tcattgatga
    12121 gctgacggtt tcttataacc gtgcaagaca aggggctatt acccaagaaa ttaccgaaat
    12181 tgttgccgga gcttcggcac tagaatagta ttgagattgg aggaaaaaag atgagttcag
    12241 gcaagattgt tcaagtaatc ggtcccgttg tcgacgtgga attttcttta gaccaatcct
    12301 taccagatat caacaacgca ttagttgttt ataaaaatga cgaaaataaa tcaaaagttg
    12361 ttcttgaagc agcgttagaa ttaggggacg gagtgatccg gaccattgcg atggaatcaa
    12421 cagatggttt acaaagagga atggaagtca ttgatacagg caaagcaatc tctgttcctg
    12481 taggtaaaga aacattaggt cgcgtattca acgtactagg agatacgatc gatttagaaa
    12541 cacctttccc agaagatgcg gaaagaagcg aaattcataa aaaagcacca gcctttgacg
    12601 aattaagtac aagtacagaa attttggaaa cagggatcaa agttatcgat ttgcttgccc
    12661 catatttaaa aggtgggaaa gtcggactat tcggtggtgc cggtgttggt aaaaccgtac
    12721 tgatccaaga actgatccat aatatcgccc aagaacatgg tggtatttct gtatttaccg
    12781 gtgtaggtga acgtactcgt gaaggtaatg acttgtatta tgaaatgaaa gattcaggag
    12841 ttatcgaaaa aacagccatg gtgttcggac aaatgaacga accaccaggt gcacgtatgc
    12901 gtgttgcttt gactggtttg acgattgcgg aatatttccg tgatgtagaa ggtcaagatg
    12961 tactgttgtt tatcgacaac attttccgtt tcactcaagc tggatctgaa gtatcagcct
    13021 tgttaggacg gatgccttct gcggttggtt atcaaccaac attggcaaca gaaatgggtc
    13081 aattgcaaga acgtatcaca tctacgaaaa aaggttcgat cacatcgatc caagcaatct
    13141 atgtgccagc cgatgactac actgacccgg cgccggcaac agcattcgcc catttggatg
    13201 cgacaaccaa cttggaacgt aaattaacag aacaagggat ttatccagcg gtagatccat
    13261 tggcttcttc ttcaagtgct ttagcacctg aaatcgtggg agaagaacat tataaagtag
    13321 caacagaagt ccaacacgta ttgcaacgat atcgtgaatt acaagatatc attgctatct
    13381 taggtatgga tgaattatcc gatcaagaaa aagtgttagt ttctcgtgcc cgcagagtcc
    13441 aattcttctt gtcacaaaac tttaacgtgg ctgaacaatt tactggtctt ccaggttcat
    13501 atgtccctgt agaagaaaca gtgaaaggat tccgcgaaat cttggaaggc aaatacgatg
    13561 atcttcctga agaagctttc cgaagtgttg gccgaatcga agacgtcgta gaaaaagcga
    13621 aaacactagg ctactagaaa ggggtgcccc atggatcaat atttaacagt aaatgtggta
    13681 actcctgccg gtttggtcta tgatcaccat gcagctatcg tcgttgcacg tacgacagct
    13741 ggtgagcttg ggattttgcc taaacatgca ccaatcatcg ttccattgac gattgatgaa
    13801 gttcgtgtaa aacgaacaga ttcagatacc cacgtcgatt ggatcgctgt taatggcgga
    13861 atcatggaag ttcgtgataa tgttgtttca atcgttgctg atagtgcgga acgtgaacgt
    13921 gatatcgatg tatctcgtgc ggaacgtgca aaacaacgcg cagaacgcca aatcgaagaa
    13981 gcaaaagcaa aacaagatac gaacgaattg aaacgggcaa ctgttgcgtt gcaccgagca
    14041 atcaatcgta tcaaagtatc taagcactca taaaatgaga tgcggatgtt tatccgtgtt
    14101 taagaacagt aaaaagaggt tatgacagaa gtggtcagct tcaagacata agaaggaatt
    14161 tacgaaaatt gcttctcaaa tttttgtgaa atctcagctt atttttcgaa gaagctactt
    14221 ctggaacacc gtatatcaga ggttgtgaaa tcagcgtttt gtcctgagta ttagagaaac
    14281 aaatggaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagatga
    14341 ttgaacaccg tttatttgga ataagggact gcgacaagac ttttgtctca gtcccttttt
    14401 ttgcatattt ttagcattta tttcctaaaa aatgtaaggg gattcctata tttttaagta
    14461 tacttgtttt tccttttata cattttatgg tattatcatt ttattagtta ttataaaaca
    14521 atcgttagaa aggacgtcat tatgcaagta tacggaattg atgcggtgct tcgaatcgtg
    14581 agtcatttgg cgtttatcta cttagccttc tggtctttac gttcgttacg cattgaaact
    14641 tttttcagac caatgcataa tacgcaaatt agaatggcaa tcgtactttt ttcaattttt
    14701 ttagggtaca ctgcaagtaa ttttttctta gaggttatcg ctttatgccg aaatcttttc
    14761 gttagcctgt aaattttaaa aaaaggtact attttaaaag caaggatgtt ataatagaaa
    14821 agattttgag taagtgtatg ctcattggaa ttttttggag ggaacaacat ggaagagatc
    14881 atcgtaagag gtggcaatca attaaatgga acagtaagaa ttgaaggagc taaaaatgct
    14941 gttttgccta ttttagctgc tagtttgtta gcagaagaag gtattacgac attagacaac
    15001 gttccgattc tttctgatgt cttcactatg aatcaagtga ttcgtcactt aaacgtagat
    15061 gtagattttg atgaacaaaa gaatcaagta acaatcgacg cttcaagaca attagaaatc
    15121 gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta
    15181 cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat tggtaaacga
    15241 ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat tcaaaaaaac
    15301 ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt agatttccct
    15361 agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc
    15421 attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt gaataaaatg
    15481 ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt ggatcattta
    15541 catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt tatggttgct
    15601 gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca taaccgccca
    15661 ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg cggagtccgt
    15721 gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca tcctggtttc
    15781 cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg tacaagtgtg
    15841 gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg ccgtatgaac
    15901 gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga attgcaaggt
    15961 gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc tggcttgaaa
    16021 gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata ttataatttc
    16081 catatcaaac tacagcaatt aggtgcagac gtcgaacgtg tagacatgga tcaaacttct
    16141 gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa ccaacgatat
    16201 atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct tctttttgtg
    16261 gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa agtattccag
    16321 ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat cacagtggtt
    16381 ctagtcgata tgaagtgagc catgaagcag aacatgataa aagaaaacag aagggaacgt
    16441 tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa ttgatgaaga
    16501 agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca tttccaccta
    16561 gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa acccatggtg
    16621 cgctaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct tttgtcaaag
    16681 gcggtatcga ttatgtacca ttgacattcc gattaacgcg aaataagaaa gataaaaaag
    16741 aaccagatta taaaaaggtt gtgaaaaata cgggtagcac tgagcgataa agaaaaagag
    16801 gaaatttatt gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg
    16861 aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag
    16921 aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa
    16981 caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag
    17041 taaccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga
    17101 agctttcaca gctagcttca aaccaaactc ggatcattat acgtactggc actactagta
    17161 gcgctgcgtt acacctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact
    17221 attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag
    17281 tgattttagc aattcactta cctaaaaata gctttaacta ataaactttc aattcgagaa
    17341 aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata
    17401 tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg
    17461 atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga
    17521 aaacgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg
    17581 gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga
    17641 ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt caactcccag
    17701 taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc
    17761 gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca
    17821 cgaaaggaca aataaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct
    17881 tagatggcga taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt
    17941 gtgaacgcaa aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag
    18001 aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg
    18061 gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt
    18121 ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat
    18181 tcgatctgca tgatttcgaa ccgcagattt tattatgtga gtacagaaac gatgctaatc
    18241 ttataggtgc agcagcaaac tatctcgctg tacgtcaagg agagaattaa gagctttaga
    18301 gaaggtgcag cttgcataaa agaagagaat gaaatgaata ggagattttt acctaaggaa
    18361 tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa
    18421 taaagatatt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga
    18481 agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct atgggccaaa
    18541 agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc
    18601 gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt
    18661 caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa
    18721 acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg
    18781 ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa
    18841 gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt
    18901 aagctctcgt tagttcgaga gctctttttt tttgctttgc taccttgttc cttgaagatt
    18961 ctgctgaatt aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg
    19021 aaagaaaaag tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac
    19081 aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc
    19141 ccaacgaatc atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat
    19201 aagtcggcat tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa
    19261 aaaattgttt ctgacaatag gagtagggag aacgatgtgt gtctaaatcc tctgttgatg
    19321 gatagaaacc tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa
    19381 acgaaaggac gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac
    19441 caattaaaaa atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa
    19501 agagaattag cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac
    19561 ttggcagatg aaggaatctt agaaagaaag atcggttcgg gtacgtatgt tgcgcgagaa
    19621 aaagtacaag aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcacaaaat
    19681 cgagtaccgt ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg
    19741 gaaaaactaa acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca
    19801 gatgatgtcc ctatttgttt tgaagttgcc agtgttccac aaaagctgat cgatggctac
    19861 agcaaagcag agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag
    19921 atcggtccag cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat
    19981 ttagaggtaa aacgaagaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac
    20041 ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg
    20101 gaaaaataaa aaacaggtga tccctgattc cttattagaa ataaaagaga atcagaatca
    20161 cctgttttta tatatgatca gttatcagtt gctcgtgata agaaacagaa ccctcttcaa
    20221 attggacttg tccgttcagc agctcaatga tggattcttt aaagtggtta gcactttttt
    20281 catctacagc acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat
    20341 gttggagttc aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga
    20401 tctcctgttg caaggtacct tctacgattc caatgtcaga caatgcttga gagaccgcat
    20461 gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga
    20521 ctactgcagc tacattgatc agttgatttt ttttgaggac ttccaacatc ggtacgcccg
    20581 ccgttccact tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga
    20641 tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaat
    20701 tttttgcttg gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga
    20761 tctcaatctc actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac
    20821 ctcttttttt cattttcttt attcagtata aataaagttc taggttgttt caacactgta
    20881 acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg atctggggat
    20941 gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc
    21001 tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc
    21061 caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg
    21121 tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata
    21181 tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta
    21241 gaaaacgttg acaaatttct ttctttttat ataataaagt aacagacgcg aagaaaacgt
    21301 ttgcgtagtt tgttttaaaa aggagagaaa aatatggcta aaaaaacaat catgcttgta
    21361 tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca
    21421 aaaggaatcg actctgatat tttcgctgtg tccgcttctg acgcagataa caatcttgca
    21481 aataaagacg tggatgtgtt gttattaggc ccacaagttc gctttatgaa agctgatttt
    21541 gagaaacgtc tggaaccaaa aggcattcct ttagatgtca tcaatatggc tgattatggt
    21601 atgatgaatg gcgaaaaagt gttagatcaa gcaatcgcgc tcataggtga ataaatagaa
    21661 cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc gtatattttt
    21721 ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga gatattaaag
    21781 caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca gtggaacgtt
    21841 tgggcaactg gattctttgt catcgctgtg gaatgaggac gtccgtcatt gttggacagc
    21901 ttgcagatta cggttatttt tgtacacatt gtctatattt agggtgatgc gacactcaac
    21961 aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc tttttttgga
    22021 caggacagct tactgaaaaa caaacggaaa tcgcaaagag aatactttgc cattcagaaa
    22081 aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg ctttatccta
    22141 ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg
    22201 tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc
    22261 tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt acgattcatc
    22321 agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc gatgcgtttc
    22381 cttataacgg ggatgctggg ctagctcacg ctgtgcaaac agcaatcaga tcaaacggcc
    22441 gattcatcta cttaagtgcc acgcctgatg agaagttact aaaggaaatc aagcagacat
    22501 ttgagctcca caaacttgcc ttgcgctttc atcggcgctt gcttcctgaa cccgtgcttg
    22561 ttttcatgaa taattggcga caaaaatgtg gaaacaaaaa gaaaatcaac tctttggtgc
    22621 ggatcattca gcaactaatc aaagaaaacc gtatcctttt attttgtcca agtattacga
    22681 tgatgcatcg tttaaaaatc acactgcaag aagcttttcc agaatatgag ataacagatg
    22741 tttcttcaca agacgatcaa cgggcaaaga aagttcagaa aatgcgagaa gcaatatacg
    22801 agatactgtt aaccacaatg atcctagaaa gaggagtcac atttgaaaga atctctgtca
    22861 tcgttttagg agcagatcat ccagttttta ctaaatcctc tttggtgcaa atcgcaggaa
    22921 gagctgatcg taaaggtcct tatacgaaca gtcgagtcta ttttttctat gaagaaaaaa
    22981 cttcagctat tcgaaaagcc tgtcaggaaa tcaaagaaat gaacgaagaa gggaagcaga
    23041 tgctgtgaga tgtagttgct gcggtaaaga catcattcaa aatttaacat tcagcgagat
    23101 catcggagtg tcgaaatcta gacttcctca gacatgttca gtttgccgcg cacaattcca
    23161 tttattagaa gcaaaaggtt gtgcaggttg tcaaaaacca atccatttga aatacgagga
    23221 gacgaaaaag aaagcaaata ctagtttcgt ttactgcaac gactgtcaag aatggaagaa
    23281 gaaatatcct gcatatgatt ttcaacaccg cgcctttttt acgtacgatg aaaagatgca
    23341 ggcttggatc aatcggtaca agtttatggg agatatccga ttagcaggga cttttgcata
    23401 taaatggact cagataaaaa aaagattacc cggcatatat ttattgtcca atacctttat
    23461 ctgaaaaaag gtggctagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa
    23521 aaattccttt tcagatgctg ctcaaaagag agcaacatct gcttgctcaa gcaaaaaaca
    23581 cgcgaaagga aagattggag atgcctcagc cttttgagct tgccgtacct ctttctaaga
    23641 tacaacataa aaaa
//
LOCUS       DAZULG010000036        23426 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000036 DAZULG010000000
VERSION     DAZULG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.036"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1712
                     /locus_tag="ACG9CY_001255"
     CDS             51..1712
                     /locus_tag="ACG9CY_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4937775.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1782..2129)
                     /locus_tag="ACG9CY_001256"
     CDS             complement(1782..2129)
                     /locus_tag="ACG9CY_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS4937776.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2213..3154
                     /gene="opp3b"
                     /locus_tag="ACG9CY_001257"
     CDS             2213..3154
                     /gene="opp3b"
                     /locus_tag="ACG9CY_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4937777.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3155..4198
                     /locus_tag="ACG9CY_001258"
     CDS             3155..4198
                     /locus_tag="ACG9CY_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4937778.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4204..5274
                     /locus_tag="ACG9CY_001259"
     CDS             4204..5274
                     /locus_tag="ACG9CY_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937779.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5264..6217
                     /locus_tag="ACG9CY_001260"
     CDS             5264..6217
                     /locus_tag="ACG9CY_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937780.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6229..7239
                     /locus_tag="ACG9CY_001261"
     CDS             6229..7239
                     /locus_tag="ACG9CY_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4937781.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7524..8345)
                     /locus_tag="ACG9CY_001262"
     CDS             complement(7524..8345)
                     /locus_tag="ACG9CY_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4937782.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8442..8720)
                     /locus_tag="ACG9CY_001263"
     CDS             complement(8442..8720)
                     /locus_tag="ACG9CY_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937783.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8988..10997
                     /gene="metG"
                     /locus_tag="ACG9CY_001264"
     CDS             8988..10997
                     /gene="metG"
                     /locus_tag="ACG9CY_001264"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS4937784.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11476..12063)
                     /locus_tag="ACG9CY_001265"
     CDS             complement(11476..12063)
                     /locus_tag="ACG9CY_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937785.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12238..13560
                     /locus_tag="ACG9CY_001266"
     CDS             12238..13560
                     /locus_tag="ACG9CY_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4937786.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13715..14491
                     /locus_tag="ACG9CY_001267"
     CDS             13715..14491
                     /locus_tag="ACG9CY_001267"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4937787.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14484..15053
                     /gene="rnmV"
                     /locus_tag="ACG9CY_001268"
     CDS             14484..15053
                     /gene="rnmV"
                     /locus_tag="ACG9CY_001268"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS4937788.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15046..15930
                     /gene="rsmA"
                     /locus_tag="ACG9CY_001269"
     CDS             15046..15930
                     /gene="rsmA"
                     /locus_tag="ACG9CY_001269"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS4937789.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16381..17010
                     /locus_tag="ACG9CY_001270"
     CDS             16381..17010
                     /locus_tag="ACG9CY_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937790.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17134..18243)
                     /locus_tag="ACG9CY_001271"
     CDS             complement(17134..18243)
                     /locus_tag="ACG9CY_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937791.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18452..18874
                     /gene="mgsA"
                     /locus_tag="ACG9CY_001272"
     CDS             18452..18874
                     /gene="mgsA"
                     /locus_tag="ACG9CY_001272"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS4937792.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19061..19159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19197..19733
                     /locus_tag="ACG9CY_001273"
     CDS             19197..19733
                     /locus_tag="ACG9CY_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4937793.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19773..21536)
                     /locus_tag="ACG9CY_001274"
     CDS             complement(19773..21536)
                     /locus_tag="ACG9CY_001274"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937794.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21539..23254)
                     /locus_tag="ACG9CY_001275"
     CDS             complement(21539..23254)
                     /locus_tag="ACG9CY_001275"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937795.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7545 a         4290 c         4771 g         6820 t
ORIGIN      
        1 cttcagaaaa tattaattat tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga
       61 aatttacatt tggtttggtt gcggtatgcg gccttgtact tgctggatgt tatggcggca
      121 gttcagccac aagcagtagt tcagcaaaaa gcggttcagc agatggagga ggcgtattta
      181 acttagtcgt gcctcaagaa atgcctacag ctgacttatc agttgcaaca gatacaatca
      241 gttttacagc attgaataac gtttatgaag gaatttaccg tcttgatgaa gacagcaaac
      301 cacagccggc aggagctagc gagttggcag aagtcagtga agacggatta acttacaaaa
      361 tcaaattacg agaagacgca aaatggtcaa atggtgatcc tgttacagca gcggattacg
      421 tttatggctg gcaacgaaca gtagacccag ctacagcttc tgaatatgct tacctgtatg
      481 cgccagttga aaatgcagaa gacattacag ctggaaagaa agataaatca gaactaggga
      541 tcaaagcagt tggtgactat gaattagaaa tcaaattgac gaaacaaaca ccatatttcc
      601 aatatctgct tgctttccca tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg
      661 gggatgccta tgcatctgca agtgataaag ctgtttataa tggaccattc acattagaag
      721 attttgatgg accaggaaca gatacagaat ggacttataa gaaaaatgat cattactggg
      781 acaaagatac agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa tcttctactg
      841 cattgaactt attcaaagat ggccaagcag atgacgtgat cttgtctggt gagttagcac
      901 agcaaaacgc aaacgatcca gcttatactt ctgttaaaga agcgcggaca agctatatcg
      961 aattcaatca acgtgaaaag aattcaccat tcaacaatgt gaacctaaga aaagctattt
     1021 cttattctat cgatcgtgaa gctttagtaa aacaagtact aggtgacgga tcagttgtct
     1081 ctacaggatt gatcccatca gatatggtaa aaagtccaac aacgaacgaa gactttgcaa
     1141 aagaagctgg aaaattagtt tcttatgaca aagataaagc aaaagaatat tgggaaaaag
     1201 cgaaaaaaga attgggcgtt gattctttag aatttgattt gatggcttct gatgatgatt
     1261 catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca agaaaacttg gatggcgtaa
     1321 cagtcaaacc aacacctgtt ccattctctg tacgtcttga ccgctcaact tcgggtgatt
     1381 ttgacgcagt actaggcgga tgggctgctg actatgcaga cccaagcagc tttactgatt
     1441 tgtttgtcac aggtaattca tacaaccgtg gtcgctggag caatgcagaa tatgacgaag
     1501 cagttaaagc agcttcatca acagacgctg gcgatgaaca agcccgttgg gatgatttcc
     1561 aaaaagcaat caagatcatc tctgatgata tgggtgtcgt tccggtgtac caaaaagcag
     1621 aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat
     1681 gggattacaa atggacatac gttgaagaat aatcgttaat accgtgagca tgagaaaaaa
     1741 cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca cttatcctaa caaagaaaaa
     1801 agcatgaaaa tagcagaaag agcaattagt attccggcaa tgatcagcca aaagctgtac
     1861 ataccgtagc tggcagacga gagcgtggaa aattctggtt ttttctttcg gttccttgtt
     1921 cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa
     1981 aaaccgccga taatcagaaa cggcaatgac cataagaata gatcattaga taattgaatg
     2041 agtgtaaagg aatggttgat gagatttttt attaaaataa ccagaatcag tagaaaagct
     2101 actgcgtagg ggatagattt ttttttcatt tgattgctcc tttatttttg gttcattatc
     2161 taataatgta gcgaaacatc tgtcagaagt caaaagctag ggggaaacat tagtgaatag
     2221 ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc atcacgcttt ggttaattgc
     2281 aacaatcacc tttttcttga tgcaattgct gccgggcacg ccatatacga atcaggaacg
     2341 tttgagtcct gaaacaatcg agatgctaaa taaacaagtc ggtttggata aaccagttat
     2401 cgtacaatat gggatttatc tttctaattt gcttcaagga gattttggaa tttccttcca
     2461 atttaaaaac cagccagtcg cacatttgtt agctggaaga atcggaccat ccttgcagtt
     2521 agggttacag gcaattatct ttggaacagt cctagggacg attttaggaa cgatctctgc
     2581 catgaagcaa aatacatggg cagatacatc ttctacactc gtagcgatat taggtcgttc
     2641 cattccaaac tttgtatttg ctgttttatt acaatatatc tttgcaatca aattacaagt
     2701 tttgccaatc gcaaaatggg atggctttgt gtacacaatc ttaccaacaa ttgcgttggc
     2761 catgtcgccg cttgctgact ctgctcggtt tatccgtacg gaaatggtgg aagtactgca
     2821 tagtgattat gtggaattag caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa
     2881 acatggtctt cgaaatagtt tgattccatt gatgacttta ttaggaccgt tagctgtagc
     2941 attgatgacc ggatcactgg tggtagaaaa tatctttgct attcctggta tcggggaaca
     3001 attcgttaag tcaatcacaa caaatgatta tcctacgatc atggctgtaa cgatccttta
     3061 ctcatttatg ctgatttttg ttattttgat cgtggatcta ttgtacggtc tagttgatcc
     3121 aagaattcgt gtttcagaag ggagccgagg ataaatggaa atgataccaa agcgttatga
     3181 aacagttgca gatatccctg cggatgaatt tcttccatta caaaaaaata ccgaagaaga
     3241 acgtgaacag attactgcac cttctttgaa ttttatccaa gattcatggc gccgtttaaa
     3301 gaaaaacaaa gctgccgtta tttcaatggt tttactattg gtcatcattt tgatctctgt
     3361 gatcacaatc tttgtttctc ctcatgatcc aactgcccaa aacgtggctt atatcaattt
     3421 gcctcctcgt attccgggaa tcaatatcga tggattgaat ggaaaagcaa tggttggtgg
     3481 tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg
     3541 aacagacggt ttgggacgtg atgtattaag ccgcttattt atgggaatga gaatctcgtt
     3601 gctgatcgca tttattgctg cattactaga tgtaacgatt ggtgtagctt acgggttggt
     3661 ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc
     3721 aggaattcca aacctcgttg tcatgatttt gatgttggtc gtttttgaac caggaatttt
     3781 ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca atggcacgta tcgttcgtgc
     3841 acaaacaatg aaattaaaag atcaagaata tgttttagct ggattgacat taggtgaatc
     3901 aagaatgaaa attgcattca aacatatttt gccgaatatc tctagtgtca ttattattca
     3961 aatgatgttt agtattccat ctgctatttt cttcgaagcc tttttaagct ttatcggttt
     4021 aggtcttacg cctccgtcag cttcattagg aacgatgttg agcgaagggt acaagacatt
     4081 cctgtatctg ccttatctat tatggattcc ggcagtaact ttatcggtta tcatgattgg
     4141 ctttaactta cttgctgatg gattgcgtga tgcctttgat cctaaaatga aagagtgaag
     4201 acgatgacga aaattttaga agtgaaagac ttagaaatct cttttgatac gtatgctgga
     4261 aaggtacgag cgattcgcgg tgtcaatttt catttgaata aaggagaaac attggcgatc
     4321 gttggggaat ctggaagtgg aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca
     4381 agcaacgcca atatagatgc tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa
     4441 tcagaaagag agatgcaaaa aatccgtggt aaagaaatag cgatgatttt ccaagatcca
     4501 atgacctctt tagatccgac gatgcccatt gggaagcaag tcgctgagtc ccttagaaag
     4561 cataataaag tttcaaaaaa agagggacaa aaagctgcat tagaattgct taaattagtt
     4621 ggaatacccg aagctgagaa acgtatcaac agttatccgc atcagttctc aggaggtcag
     4681 cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc cggaaatcct gattgctgat
     4741 gaacctacaa cagcgcttga tgtgactatt caagcacaga ttttggagct gctgaaagaa
     4801 attcagacaa aaatcgatac atcaattatc tttatcactc atgaccttgg tgtagtagcg
     4861 aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg
     4921 gaagaaattt tctacaaccc gcaacatccc tatacttggg gactgcttgg ttcaatgccg
     4981 acattagata gtgcgaacga tcgattgtat gctattcctg gttctccacc ggatttattg
     5041 aacccgccaa aaggagacgc attctatcca agaaatgaat tcgcgatgaa aatcgatgca
     5101 gaacaagaac caccattttt tgaactatca aaaacacaca aggcagcaac ttggctactt
     5161 gctccgcagg cacccaaagt gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt
     5221 aaagagaaac aaaaacatac ttacggtcaa ggaggaattg ctgatgccaa ataaactact
     5281 tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt
     5341 aaatgacatc agctttcaca tttatgaagg agaaacattc ggtttagttg gagaatctgg
     5401 aagcggaaaa tccactactg gccgtacgat cattcgcttg aatgaaccta ctgatggtga
     5461 gatattattc gacggtcaag atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt
     5521 ccggcatgat gttcagatga tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa
     5581 agtcagagat atcattgccg aaggaattga tgttaatgga ctagctaaat catctgaaga
     5641 gcgtgcaaaa aaagtcgatg atctattgag aactgttgga ttgaatccaa gccacggtac
     5701 tcgttatcct catgaatttt caggtgggca acgccagcgt attggtattg cacgagcatt
     5761 ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt tctgcactag atgtgtcgat
     5821 ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa gaacatcaat tgacctatct
     5881 atttattgca catgatttgt cgatggttaa acatattagt gaccgaatcg gtgtaatgca
     5941 taacggcttg ttattagaga tgggaacaag tgatgaaatc tataatcacg gtgttcatcc
     6001 ttatacagaa agtttgttat ctgctattcc tctaccagat ccagaccatg agagaaaacg
     6061 cagaagaatc aaatatcaac ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat
     6121 cgcaccagaa cattttgtct atgcaactga gcaggaagtc ccatattacg caaaaaaact
     6181 aaaacgacaa aaggaatctc ttctggttgc taactaggag ggacatagat gctaaaagct
     6241 tttaaattcc gtatgtatcc gacagaagaa caaaaacagc aattgattcg tacttttgga
     6301 tgtgctcgat ttacctataa ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca
     6361 ggagtagcca atttttcgct cacacccgct acattaaaaa aagaataccc attcttaaaa
     6421 gaagtagaca gtcttgcttt agcaaatgca cagcttaatt tggatcgggc ttttcgcaat
     6481 tatttcaagg gacgtgccag cttcccaaag cttaaaacga aaaaaagtat gtggcaatct
     6541 tatacgacca acaaccaaac ccgtacgatc tatctggaaa atggctactt aaagttacca
     6601 aaacaaaaag aactaatcaa gataaatcag catcggccag ttgaaggatc gattcgctcc
     6661 gctacgattt ctgctcgata taatgaagaa ttttatgtcg cactactttg tgatgtttcg
     6721 ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg cctatcatcc gaagacattg
     6781 atccaaacat ctcgacctct agaagtaact ttaccaaaat ttcaccagac agaagaaaag
     6841 ctgcaacatg cacagcggaa attgaatgtg aaagtccgct ccgctcacca tagaaagatc
     6901 aggttagatc aggcaagcaa ttatcagaaa caaaagagaa aagtaatgga tctttatctg
     6961 aaacaaaaga accagcgaga agattatctt gaacaattat ctgggaaatt ggtcaaacaa
     7021 tatgactact tatttgtaga atcttttcca aaagaagagg cacatgctga tttttctatt
     7081 cacgactggc ataaattaat aacaaaattg cgttataagt cccaatggta taataaaaaa
     7141 tttcttttga tcaataccga cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta
     7201 gtagaaaaaa tgggcagaca ttcggttatc aagggataaa aaatgaatga aactgatagg
     7261 agcgttaggg ttagccttgt taataaaaag tcttaatgac ttttgtttct aggaattttt
     7321 cttttgaaga aaagctcact aattatgaca aaccaataaa acaatagcag aaaaagttcg
     7381 tacgagcttt ttctgctatt gttaattttt tattcagaaa gtttacgaat acatcaaaat
     7441 tctgaaacaa aaaacgtaac tcacaaagag ttgtttttgc gttttttctt gaatgattag
     7501 gtctgaatct tttgctcaca accttattca tggtgccccg gcttgagctg atagattttt
     7561 gaaggacggc cgattcctac aggacgttca ccgatctcat cgaaatattg gagcatagct
     7621 ttcttaaaat tggaatgatc gatcgtttta taatcgatcc ctaagaattt agcaaatact
     7681 tttcgagctt ccgtaatcgt gaaatcttta cctaaaactt gtagaacttg cggctcgtgt
     7741 tccattttat tgactacacg gttgaatgct ttcaagatga tttgactatg gtcaaaagcc
     7801 aaagtatctt tgccagaaga ctctccagtg actaaatcaa gcgagatagc cacttctccg
     7861 ctagataaat ggatcttgtt actatgacgt tctagtgtga accagcgtac ttccttggca
     7921 tcatctccgg cgattagcgg ttcttctcca ataaaggcta agtagctgac tgtgacgacc
     7981 cagcctcgcg gatctcgatt aggcgtactg aatgtatgca gttgctcaat attttctttt
     8041 gaaataacca caccagtttc ttcctttgtt tctcttaaga cgctttctcc agttgactca
     8101 tccttttgga caaagccacc aggcaatgcc catgaatttc gataaggatg cccttttctt
     8161 tggatcaaca aaactttcag ctgatcttct tctttgttat agcacatcag tacgatatcg
     8221 acagtcaaag agggcttgtc atattctgga cgttcctgtt tttgatacca gttaagaaat
     8281 tcttcttcac ttgctttatg ttcataatat aatttttctt cttctttaga agcaaaacgg
     8341 ttcatctgac cacctccatt gacagtaaaa gtacttttta ccttattgta gcagactctg
     8401 aaaaaaaacc aaggatttac ttgaaaaatc taggttatca tttaaatatg aaaaataaat
     8461 ggaagtatga gattgaataa aaagaaaagc agataaaacg gcaatacttt ataccgctct
     8521 tcacgaatgg caaagaagcc acaaataacg atatatacgt caatgataaa acgcaaaatc
     8581 caagctgctg tcataaaact gttcaaatgc ccatggaggt taaaataacc atacatacga
     8641 atataaaaat agctgaatat taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa
     8701 aaaagatgtt tcttctgcat gattgttcca gtcccttact aagatagata gtctttatta
     8761 taccacgtgt gaggggagaa ataaaaagcc gttttttagt agatatgaaa caaaacaaaa
     8821 acccgcattt attcaaccta ttcatccttt taaaggatag attgaacgta gatagaagtc
     8881 gtttatccag tctttttcat tttgaaaaaa agctaacagc atgctttttt tatggtatac
     8941 tagcaaggaa acgaattcgc atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg
     9001 aaacttttta tatcactacg ccaatctatt atccaagcgg caaacttcat atcggcaact
     9061 cgtatactac gattgcatgt gacgcaattg ctcgttataa acggttgatg ggctttgatg
     9121 tgttttatct cactggagtg gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac
     9181 taggtgtaaa accgcaagaa tatgtagata aaatggcggc tgatgtcaag aaattatgga
     9241 aaacgcttga tatcagttat gataaattca ttcgcacgac agatgattac cataaagcag
     9301 ctgttcaaaa aatatttgat cgtttattag aacagggaga tatttactta ggtgaatatg
     9361 aaggctggta ctctgtttct gacgaagaat tctttacaga aacacaactt gcagaagtgt
     9421 acaaagatga cgaaggtaac gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat
     9481 tggtaaaaga agaatcatac ttcttccgca tgagcaaata cgcagaccgt ctattggaat
     9541 actatgaaga acatcccgaa ttcatccagc cagaatctcg taaaaacgaa atgatcaaca
     9601 actttatcaa accaggatta gaggatcttg cagtttcacg aacaactttt acttggggag
     9661 taccagtcaa gagtgatccg aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt
     9721 atattactgc actaggatat ggctccgaag atgaaagtct attcgaaaaa tactggcctg
     9781 cagatgttca gatggtcggt aaagaaatcg ttcgctttca tacgatctat tggccaatca
     9841 tgctgatggc ccttgatttg cctctaccta aaaaagtatt tgggcatggc tggctattga
     9901 tgaaagacgg aaaaatgtct aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg
     9961 aacgttatgg tttagatgca ctccgttatt atttgcttcg tgctgttccg tttgggtcag
    10021 atggtgtctt tacgccagaa gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc
    10081 tagggaactt attgaatcga acgattgcta tgatcaacaa atattgtgat ggactcgttc
    10141 ctgactatgc ctcacttgtc actccatttg atagtgaact gtctacgacg gctgctaacg
    10201 ttgtcggacg ttaccatgat gcaatggaaa aaatggaatt caatacagca ttggctgaaa
    10261 tctgggtgct gatttctcgt gcaaacaaat acattgatga aacagaacca tgggtattag
    10321 caaaagacga agaaaagaaa gctgaattga atagtgtcat gatccattta gctgaatctt
    10381 tgcgtatcgc agcgatatta cttcagccaa tcatgacaga aacgccagag aagatcttca
    10441 atcaactagg attggactct gaaacaatga atcttgaagg attacacttt ggcgaatttc
    10501 cttcaggaac aaaagtagtt gcaaaaggta caccaatctt cccacgttta gatatggaag
    10561 aagaagttgc ttttattcaa gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag
    10621 tcaaatggga tccagaagaa acagaacttg tttcaacgaa agaaaaacaa atcaaatttg
    10681 atgtatttga aaaagtagaa ctaaaagtag cagaagtcat caactgtcaa aaagtagaag
    10741 gggcagataa gttgctgcaa ttccgactag atgctggaga cagccaagac cgtcaaatct
    10801 tgtcaggaat cgctgaattt tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca
    10861 ttgtagcaaa tctgaaacca cgaaagatgc gtggacaaat cagtcaagga atgatccttt
    10921 ctgcagaagc accagatggc tctttacaag taatcgaagc accaaaatcc atgccaaatg
    10981 gctcagaaat cgcatagaaa ttcaaagggg gtatgacact agttgtgtca tacccccttt
    11041 ttccgaaatt tgagaagcaa ttttcgtaaa ttccttctta ttacttgaag ccaatcatat
    11101 aatacagaaa cagagaacta tccaaaataa atcctagaaa aagaagtacg agcttcgctg
    11161 atttttgtta atttagaata atactaatag gaatcgtaaa aacagaaaaa aagcataatg
    11221 ataaagggtc gggcagattt taattgattc atctggtact cttttttatt gtccttacat
    11281 tcataaagtt tgaatgcaat gactttatta catctatatt tatatctaac agtagcgttg
    11341 atgtgttaat tataagttct atcactttat gttcaattag tttgtgctct tatatgtgtg
    11401 attgaaaata tagataaaaa aacaatcatc ggctttgact tcgatgattg ttttctttat
    11461 tttgtaaaaa tcctatcact tgattttatg gtaacctttt atttttgtct tcaaacggct
    11521 tggtaagaga gcagtaagca gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg
    11581 tccgatacca taatgttgat agagctttgt caacatcacg tgtaacaaga tcaaccctaa
    11641 aagcaaggag ccagctataa accaatgatt atcccaatgc atgacttcac taagtgccat
    11701 cactccaaaa ggatagaaat aaaaatagat cccgactaag gtcatccgcc agttctttgt
    11761 aaatgttccg atattcaata aaaagaaaat cgctgaaagc aaaataccga taggcggaaa
    11821 aagcccaacc aaaaaggaat acaacatccc ccacaagatc atcacaggac ctttgatcaa
    11881 tgcaacgatt gcgatgaaca aagtcagcaa tattagtttt tgtgataacg ttgcaacagt
    11941 cattaatcca aataaaatta atgtgaacca atgccaatgg gactgctgtt gttctttacg
    12001 ggaaaaatcg acccggttgg ataaataatc tgctaatttc ggataagaac gcttaaaatc
    12061 cataaactgt tcctccttcg tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa
    12121 tacatccgtc ttaagaggga gtttttcact gaaagtggag aaaaaagaat ggtaaattaa
    12181 aatattctca gttatactga aaattatacc aaatttcaaa gaagaaagag gatgggaatg
    12241 aaaggcaaac taaaacgaga aatcaatctg tttggcgcat tagctaccgt tatgggaacc
    12301 gtgatcggtg caggcgtctt cttcaaaact gctgctgtta ctgctagtac tcagtctaca
    12361 agtttgacat tgctagcttg gcttttgggt ggtttcttaa cgatctgtgc tggattgaca
    12421 gtggctgaat tagctacagc aatcccggaa actggtggag ctgtaaaata tattgaagct
    12481 gcgtatggaa aattgcccag ctttttatta ggatgggcgc aaagcttgat ttactttcct
    12541 gctaatattg ctgcattatc gattatcttt gcgacacaat tgacgaactt attacaatta
    12601 tctaccgatt atcttttgtt gattgcagtg atcaccgctg tttctgtgac agggttgaat
    12661 ttgttaggga caaaagtcgg tgcttctgta caatcagtga cgttgattgt taaattaata
    12721 ccaattgctg tgattgtcat atggggatta ttgaccccag gacaaggaac tgtccagtta
    12781 tttccaatcg aagctggaaa agatgttact tttgtcgaag gattgagcag tgcgttgtta
    12841 gcaacgttgt ttgcttatga cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa
    12901 cggccggaaa aagatttgcc taaagcaatt attcttggtt tgagttttgt aacagtcgtt
    12961 tatctattga tcaattttgt ttttttgaaa actttgccga ttgatcatct tgccggtaat
    13021 ttgaatgcag cttctgaagc atcagacgtc atttttggag gaatcggtgg aaagctggtc
    13081 actattggga tcttgatctc ggtatacggt gcattgaacg gttatacact aactgggatc
    13141 cgcgtgcctt atgcgatggc tttagaagac gagctacctt tcagtaaaca gctgacaaag
    13201 ctttcaaaga agtttacggt tccttatgtt cctgcggttt tccagttagc agtagcttgt
    13261 attatgatga gccttggttc tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg
    13321 ctattcagtt tactgatttt tatcggtgtc tttattttaa gaaaaaaagc gccggaattg
    13381 ccacgtccat ataaagtacc actttatcca atcgttccga ttattgcgat tctcggtgct
    13441 attttcattt taggtatgac gatgatgacg caaacgaaat tagcattgat cggtataggt
    13501 gttaccttga taggtatccc agtctattat caaaaaaaga aaaaacgctc agaagagtaa
    13561 aatatttcaa caacagacag aaaaagtacg aactaggtcc tctagatctt cgtgcttttt
    13621 ctatttcaat aaaacaagtt cttctatttt gatttttgct acttctgcaa gtagtgctta
    13681 tgtgctaaag taagaatgaa aaggagagat aaacatgata tttgattctc atacccattt
    13741 aaatgctgaa cagttcaatg aagatattcc agaaacgatt cagcgagcac aagaaatggg
    13801 ggtaacgaaa atggcggtcg tgggcttcga tacgccaacg attgaaaagt ctttgcaatt
    13861 aagccatgac tatccaaata tatacagtat tatcggctgg catccgacag aggctggcag
    13921 ttatacgaaa gaaatcgaaa ataaattaca agaacaattg actttaccaa aggttgtagc
    13981 attaggggaa attggcttgg attatcattg gatggaagac ccgaaagaag tccaagataa
    14041 agtatttcgc agacagattg ctatcgcaaa agaaatgaac ctaccaatca gtattcatac
    14101 tagagaagca cttgaagaca cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg
    14161 tggaatcatg catagtttca gtggtgatta tgaatgggca aaacgatttc ttgacttagg
    14221 gatgcatatt tcatttagcg gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc
    14281 tgcagcaaat gtaccaatcg aacgattact tgtggaaaca gatgcccctt atctggcacc
    14341 agttcctttt agaggaaagc gaaatgaacc aggctatact cgttatacag tagaaaagat
    14401 tgctgaactt cggaatcttc cgacagaaga agtggctgcc caaacatgga caaatgcgca
    14461 ccgattattt ggtctaagcg aacatggata agatgaagat ccaagagatt atagtcgttg
    14521 aaggaaaaga cgatacaaga aggctgcggg aagtagttga tgcagatacg atagaaacaa
    14581 tagggtctgc aatcaatgaa gagatcctga tgcaaattga acatgcccaa gagactcgtg
    14641 gagtgatcgt ttttaccgat cctgattttt caggagaaaa aataagaaaa atcattatgg
    14701 aagctgttcc tcaggcaaaa catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa
    14761 agggaagcct tggtgtcgag catgcaagtg atgctgctat tttagaagca ttgaaaaagg
    14821 tcgtgacgcc agacatttcc caaacggctg tttctgaaat cactagacaa gaccttttag
    14881 actatggttt aatagctggg gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt
    14941 tgcgcatcgg ttacacgaac ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta
    15001 cgccgaaaga actcgcccaa gcaatgaaaa tagtggagga acaacatgag tgaatatcgt
    15061 gaaattgcga caccctctag aacaaaagag attttattga aacatggttt ttcatttaaa
    15121 aaaagtttag gacaaaattt tctgactgaa ccaaatattt taaggaaaat cgtagaaact
    15181 gcgcaaatag atgaacatac gaacgtgatc gaagttggac caggaatcgg tgcattgacg
    15241 gaacaattag ccaagcatgc aaaacaagtc gtagcatttg agatcgatga tcgtttgatc
    15301 cctgtattgg ctgatacgat gcaaccctat gacaatgtga ctattgtaca tcaagatatt
    15361 ttaaagaccg atttgtcaac agctgttaga gaaacatttc atgaggagct tccgttaaaa
    15421 gtcgtagcga atttacctta ctatattacg acaccgatca tgatgcattt tcttgaatca
    15481 gatttggttg tggatgaact agtcgtcatg atgcaaaaag aagtagctga tcgtatctct
    15541 gctgaaccag gaacgaaagc ttatggttca ttatcgattg ctgttcaata ttatatggaa
    15601 gccagtttag cattcatcgt accaaaaaca gtatttgtcc ctcagccaaa tgtagattca
    15661 gcgattttga aactgacaag aagagatata ccggcagtcg aagtgacaga cgaaaaagca
    15721 tttttccgat tgacaaaagc ggcatttcag caaagaagaa aaacgctttg gaataacttg
    15781 cagcattcgt atggcaaaga tgaccagaca aaagcatggt tagcaaaaag cttggaaaca
    15841 gctggaatcg atccaaaacg tcgtggggag actttgtcat tacaagaatt cgccgcatta
    15901 agcaatgcga tgtcagaaaa tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa
    15961 agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tgtttttcgt
    16021 ttgtttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggttgtg
    16081 ggcctgccgt ttaactctaa gtcacaagga acaatttcaa aaaacagctt ctcatgtttt
    16141 tgagttagtt ggtcttatta cttgatgcaa gcttttgacc actattatgc cgaacaagga
    16201 gatgtgacac aaattttgtc acatctccta tttttgtttc ttgatcaaaa aattcttata
    16261 cttgatactc acactcattt tgtatggatt tagaaaagaa tatgtgtctt gacagttatt
    16321 agtaagtgag ttatactaag tcgacattta tgtgtcaaga catattagga gagagaaaaa
    16381 atgagaaaaa attcagttcg aaaactaacg atttctgctt tattgattgg gatgggggtc
    16441 attatcccaa tggtcatgcc taaaatcatg attccaccag cttcctttac tttggcgtct
    16501 catgtgccat tatttattgc gatgtttttt acaccaggag ttgcagtggc tgttgcgcta
    16561 ggtacgacat ttggtttctt tttaacaacg ccggttatca tcgcattgcg agcattgtca
    16621 catcttgtgt ttgcgttgat tggggcttgg tacttgcaaa agcatccgag tatcgtttta
    16681 aaaaatggac agtttacttt tgctaattgg cgtttccaag ggttcaattt tgtgatcggt
    16741 gtgattcatt ctttagcaga aatgctagta gtcagcatct tttactttat tggtaatatg
    16801 cctgaaacat attattctca aggatatttt tatacagtat tcatcttgat ggggattggt
    16861 gggcttatcc acagtttagt tgattataat attgcttatt ttatagcagg aacactcagc
    16921 aaatattttg atatccccgt atttacggca gcaaaaaaag aaaatcggaa agagtcgatt
    16981 caagggtacg cgaagaaaac gacatattag acatgatctc tcaaaaaaaa aactctcatc
    17041 tagtaatgga taataaatcc tctaagatga gagttttttg tcctaaaaag agctgcacca
    17101 acatcttctg gcacagctct aacagggggc aatttatgga agtttgatga cttgttctgt
    17161 ttctttatca aagaagtggc ctttgctgat gttgaaggca aggtcgacta tttcgcctgg
    17221 tttatggaaa tctcttgcat ctacttttga gatgaactca gtagaaccag tttttgtgta
    17281 aagcatcgtt tcagctccaa gtaattcgga aacgacaact tctgatttca ctacggcttc
    17341 aggtgaagct tcaagggcta cttgttcact gtggatgtct tctggacgaa taccgaagat
    17401 aacttctttt ccttcataac ctttttcaac taatactttg ttttttcctt caggaatacg
    17461 aagcttaagc ccatgtccat ctgtgataac accatttttc aatgtcacat taaagaagtt
    17521 catggcagga gatccaataa agccggcaac gaacatattg acgggtgtat cataaacttc
    17581 tttcggtgta ccgatttgtt ggataaatcc gtctttcatg ataacgattc ggtcagccat
    17641 agtcattgct tctgtctgat cgtgagtaac atagatggtc gttgtttcaa gacgctggtg
    17701 aagtttggca atctcagcac gcatggctac acgtaattta gcatctaaat ttgataatgg
    17761 ctcatccatt aagaagactt ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg
    17821 gcgttgtcca ccagataaag cagctggttt tcgtttcaag tattctgtca gacctaagat
    17881 ctctgctgca ttgtccacac gttttttgat gtctgcttta tcatatttac gtaatttcaa
    17941 gccaaaagcc atattatcaa aaacagtcat atgaggatac aatgcgtagt tttggaaaac
    18001 catggcaata tcacggtctt taggtgccac gtcattcatg acttttccgc cgatatttag
    18061 ttctccttca gagatatctt ccagtcccgc gatcattcgc aaagtagttg actttccaca
    18121 accggaagga ccaacaaaaa cgataaattc acggtctttg attgatagat taaaatcagt
    18181 tacagaataa ttttctgcat tgtcatattt tttatatacg tttttcagag ccatttctac
    18241 cataatacgt tccacacttt cttttttagt tttattaact atttaaagta taaatgaaag
    18301 cgttaactaa cttcaatgtc atgttgaaca agaatttttt cttttttttg tcaatgtgct
    18361 agttttaagc tttaataaat atccataaga aataaaaact gatacactgg agtaaataag
    18421 tatgttaaca atatcaaaag gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg
    18481 aaaaaaacct atgatggtga aattagcaac agcgtataaa acaatattgg aaaaacatga
    18541 actttttgct accgggacga ctgggcagag gatcacggaa gcaaccggct tgccgatcca
    18601 tcggtttaaa tccggaccgt taggcgggga tcagcaaatt ggagccatga tttcggagga
    18661 tcagttagat ttggtgattt ttcttcgaga cccgctggct gcacagcccc atgaaccaga
    18721 tgtaacagca cttatccgtt tatctgatgt ctatgagatt ccgttagcta cgaatatcgg
    18781 aacagctgag gtgctactaa gaggactgga agccggcttc ctagattttc gaagaatcgt
    18841 ccatgaaata gacaagcatc cactttcatt ttgatggata agcgtatgga aaaataacgt
    18901 ttaatgttcg cctatatgta gctataaaag ctaaacaaac tgataatatg cgatataata
    18961 cgaacgtatt ggataatata aacaataata ttagtttttc gtatttgcag taaatgtgaa
    19021 aatatgctat agtttctgtg ggaaaagatc ccaaaaataa tacagagtag aaaataaagc
    19081 gttaagtgct aagaggatgg gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt
    19141 tattttcgca ttcgctgcat gatacgatgt agcagctctt aaaggagatg ctaaaaatga
    19201 agaaacaccg gttagacgca cgtatgattg ctattatggg cttattgatt gcattgatgg
    19261 tcactttgtc ccgactggta gcaatcgaga ccccatttat taaaatcagt gtcacattta
    19321 ttccacaagt gatcatgggc attctttttg gtcctttttg gtcagggata ggcgcagttt
    19381 tagctgattt agtaggaatg gccctcttct cgaaatctgc attctttatt ggatttacat
    19441 tgaatgcatt tattgaagga gcaatctacg gattcttttt ctatcgaaaa gagatcactt
    19501 ggaaaaatgc cattctggct acgcttagtg tcacgctgat catcaactta tttttgacac
    19561 cattatggct tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc
    19621 gcctgttaaa aacagtaata tggttaccga ttcaatcaat tgccatttat tacgtaggac
    19681 gtagtattcc ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa taaaaaacaa
    19741 gcagtcttcc catcccagaa gactgcttgt ttttattgtg ctgtgaattg tgcttgatag
    19801 agccgtcggt aataaccatc tttttgaagc agctcatcat gggtgcccat ttcgacaata
    19861 gctccctgat ccatcactaa tatctggtcc gcatttttga ttgtggacag gcgatgggca
    19921 atcacaaaac tggtttttcc ttccatcatt gctaagaagg cttgttggat ttttttctct
    19981 gtcaatgtat cgactgagct ggtagcttca tctaagatca acataggggg attactgatc
    20041 atcgttcgag caatcgtcaa cagttggcgt tgtccatctg atatctttaa cccttgaccg
    20101 ccaatcttag tatctaattt ttccggcaaa cgttctacaa attcatacat ataagaggct
    20161 tttagggcat cattgatttc tgcgtcactt gcttccggat tcccaaaacg aagattttct
    20221 cgcaatgtac tgtcaaatag ccaagtatct tgtaacacca ttccaaaact tttacgtaat
    20281 gtatcacgtt ggatagagcg gatgtcatgg tcatcgactg taattgttcc ttttgttgtg
    20341 tcataaaaac gcatcagcaa attaacaagt gtggattttc cggcccctgt ttttcctact
    20401 atagcaatgg tttgacctgg ataagcagta aaattgaaat cttcgatcaa acgctgcttg
    20461 gggtcataag aaaaagaaac atgatcaaaa gagacttttc ctttgatatt tttgggttcc
    20521 aaggcatagg catctggcaa atcaggcttt tcttctggct gatcgatcaa atcaaatgct
    20581 cgttctagac cagaaaaagc ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat
    20641 ggtttagaaa actgacttga gtagatcgta aagctggaaa tcacaccaac agtgattgac
    20701 gaaccactct tcaatgccaa taggccgcca actaaaccaa tcgataaata agcaaggtga
    20761 tctacaaaac gagaagacgg gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt
    20821 tcatatagat cagcatttag cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa
    20881 gctttcacga ttttctggtt accgattcgt tcggtcacaa agcccgagat atccccaata
    20941 atcctttgct gtctatcgaa atttgtttgt gaagcacgag caacgatcca actcacaagg
    21001 aaaataattg gtgtagaaca aagaacgacc aatgtcagaa gtggacttag acgcatcata
    21061 aaaataaacg aaagaatgat gattgccact ccagaaaata attgattgaa ggccgcagaa
    21121 attgcaatgg aaatattgtc catatcattt gtaaatcgac tgacgatatt tccgtgggag
    21181 ttttgatcat aataacgaag tggcaatcga ttcagatgag caaatgcatc ttttcttaat
    21241 tttgcaacgg ataagtaagc taaccgattg cttaacactt ggatgagcca ttgactgact
    21301 acgttgatta aaacgatacc accgaataga ataagtaaat ggaacagtcg attgaagcgg
    21361 acctgtcctt ctccaatcat ttgatcgata ctttgaccaa tttgtaacgt catcacaact
    21421 gttgcaatcc cattgatgat tcctagtatc acagctagcc aaatgtcttt tgcataggca
    21481 gtaaggtaag gaatgaatcg gcggagcgtc tgcaaggata atttttttac tttcataact
    21541 attcttcctc ctgtgatgcg acgatttcct gatattctgc tgattgagca agtaattctt
    21601 catgggtacc ctgagcagca atcttacctt gatccatcac taagatatga tgtgcttctt
    21661 gaatagagcg gacacgttgg gagataataa tcactgtact gccaagataa gtctgtagcg
    21721 ccttacgcaa atccaagtct gtttgataat ccaacgcact taaagagtca tctaaaatca
    21781 gtaatgctgg cttggcaatc aaagctcttg caatcgtcag tcgctgtttt tgtccgccag
    21841 agaaattttt cccattttcc atgacttttg tatctaaacc atttggaagc tgacggacaa
    21901 attcttctgc ctgagcgatt ttcagagctt cccaacattc tttttctgtg gcgtctttct
    21961 taccccattg aaggttttca cgaatagtcc cggtaaatag tacagaagtt tggggaacaa
    22021 gagcaatttg ctgacggaga tgatccagct gccaagaacg aacatccatt ccgtccatta
    22081 taactgtacc ggaactgaca tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc
    22141 cgcttccggt tggaccaatg attccaagga ctgtatttcg cttcagctga aatgtaatat
    22201 tttggagaga aagaccagaa tcaggagtat aacggaagtc aacatggtca aaagcgataa
    22261 ttgcttcaga agaaaaatcg ggagtagtac tgacctcagc ttcaagaatc gaattttctg
    22321 tgtctagcac ctctttcacc cgatttcctg aagcttcggc acgtgtgaag atcacgacga
    22381 ggtttgaaac aacgatcagt gccaaaagca tttgattcat ataattgatc aaagcaagaa
    22441 cttgtccttg ttgcaagcta ccaatattta ctttgatccc gccaactgtc agcaaacaga
    22501 tgatcccgac attcatgatc aaggttgtag caggtgataa tagggcagag atattggaaa
    22561 cacgtaaata attgttcgct agatcatttg tgcttttatc gaacttctct gtttcttttt
    22621 ttgtctgcgc aaatgcgcgg atgacacgaa caccgctaag attctgacta acgtgttcat
    22681 tgagctgatc tagtttttct tgcacttttt ggtagagagg aacggttttt ttgatgataa
    22741 aaaataaaat gattgagaaa ataggcaata atgctaagaa aaacagacct acttcccagt
    22801 cgatcacaaa agccatgacc actgacccga tacttaaaaa cggtgctctg atcaataaac
    22861 gaatgaccat tgctaaagct agctgaagct gattgatgtc gtttgtcatt cgggtgatca
    22921 aggtatctgt tccaaaatga ttcaactcgt tgtgagagaa agtattgatt tttttgatta
    22981 gctgattacg taactctgta ccaaatcctt gtgaagctac tgaagcatag tattggcaaa
    23041 tgatagcaca aatcagacca atcacagaca tagcgaccat ccataagccc atttcaatga
    23101 tttttcctcg gtcattcatt tttagtccat tatcgatcaa ggaagccatc attaaaggca
    23161 ggacaagttc gaagacagct tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac
    23221 gatagtcttt tgcataacgt aataactgaa tcatatgatt cctcctatta tttatttgtc
    23281 cacaattgaa aaaacaagta ctaccacatt gtagcttagg aaatttagaa tgaaaagaga
    23341 acaagccttt tcataaatga aagttttatt atgaagaagc tttagaaatt gaatagacta
    23401 ttttttttaa aaaaaagaaa atgaga
//
LOCUS       DAZULG010000037        23166 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000037 DAZULG010000000
VERSION     DAZULG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.037"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG9CY_001276"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG9CY_001276"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS4937796.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG9CY_001277"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG9CY_001277"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS4937797.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG9CY_001278"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG9CY_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS4937798.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG9CY_001279"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG9CY_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS4937799.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG9CY_001280"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG9CY_001280"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS4937800.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTDPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG9CY_001281"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG9CY_001281"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS4937801.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG9CY_001282"
     CDS             complement(8053..8523)
                     /locus_tag="ACG9CY_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS4937802.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG9CY_001283"
     CDS             complement(8569..8994)
                     /locus_tag="ACG9CY_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS4937803.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG9CY_001284"
     CDS             complement(9061..9330)
                     /locus_tag="ACG9CY_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS4937804.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG9CY_001285"
     CDS             complement(9341..10936)
                     /locus_tag="ACG9CY_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4937805.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG9CY_001286"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG9CY_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS4937806.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG9CY_001287"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG9CY_001287"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS4937807.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG9CY_001288"
     CDS             15402..16376
                     /locus_tag="ACG9CY_001288"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4937808.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG9CY_001289"
     CDS             16546..17946
                     /locus_tag="ACG9CY_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS4937809.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG9CY_001290"
     CDS             complement(18024..18746)
                     /locus_tag="ACG9CY_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4937810.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG9CY_001291"
     CDS             complement(18825..19331)
                     /locus_tag="ACG9CY_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4937811.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG9CY_001292"
     CDS             19454..21253
                     /locus_tag="ACG9CY_001292"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS4937812.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACG9CY_001293"
     CDS             complement(21321..22895)
                     /locus_tag="ACG9CY_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS4937813.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6353 a         5027 c         4228 g         7558 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggatcggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaata
//
LOCUS       DAZULG010000038        23009 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000038 DAZULG010000000
VERSION     DAZULG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.038"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..2606
                     /locus_tag="ACG9CY_001294"
     CDS             135..2606
                     /locus_tag="ACG9CY_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4937814.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="ACG9CY_001295"
     CDS             complement(2709..4445)
                     /locus_tag="ACG9CY_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4937815.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="ACG9CY_001296"
     CDS             complement(4442..5146)
                     /locus_tag="ACG9CY_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4937816.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="ACG9CY_001297"
     CDS             5277..5780
                     /locus_tag="ACG9CY_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFS4937817.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="ACG9CY_001298"
     CDS             5740..6078
                     /locus_tag="ACG9CY_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS4937818.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="ACG9CY_001299"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="ACG9CY_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS4937819.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="ACG9CY_001300"
     CDS             7629..8207
                     /locus_tag="ACG9CY_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS4937820.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="ACG9CY_001301"
     CDS             8211..8732
                     /locus_tag="ACG9CY_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS4937821.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="ACG9CY_001302"
     CDS             complement(8811..9365)
                     /locus_tag="ACG9CY_001302"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4937822.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="ACG9CY_001303"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="ACG9CY_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS4937823.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="ACG9CY_001304"
     CDS             9905..10156
                     /locus_tag="ACG9CY_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS4937824.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="ACG9CY_001305"
     CDS             10281..11072
                     /locus_tag="ACG9CY_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937825.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="ACG9CY_001306"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="ACG9CY_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS4937826.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="ACG9CY_001307"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="ACG9CY_001307"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS4937827.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..12997
                     /gene="rplS"
                     /locus_tag="ACG9CY_001308"
     CDS             12650..12997
                     /gene="rplS"
                     /locus_tag="ACG9CY_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS4937828.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13370..14032
                     /locus_tag="ACG9CY_001309"
     CDS             13370..14032
                     /locus_tag="ACG9CY_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937829.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14111..17338
                     /gene="fss3"
                     /locus_tag="ACG9CY_001310"
     CDS             14111..17338
                     /gene="fss3"
                     /locus_tag="ACG9CY_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS4937830.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17452..17766
                     /locus_tag="ACG9CY_001311"
     CDS             17452..17766
                     /locus_tag="ACG9CY_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4937831.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17779..18153
                     /locus_tag="ACG9CY_001312"
     CDS             17779..18153
                     /locus_tag="ACG9CY_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4937832.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18163..18507
                     /locus_tag="ACG9CY_001313"
     CDS             18163..18507
                     /locus_tag="ACG9CY_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4937833.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18578..19930
                     /locus_tag="ACG9CY_001314"
     CDS             18578..19930
                     /locus_tag="ACG9CY_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4937834.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            19990..21132
                     /locus_tag="ACG9CY_001315"
     CDS             19990..21132
                     /locus_tag="ACG9CY_001315"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS4937835.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21157..21411
                     /locus_tag="ACG9CY_001316"
     CDS             21157..21411
                     /locus_tag="ACG9CY_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937836.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            21667..22851
                     /gene="mobT"
                     /locus_tag="ACG9CY_001317"
     CDS             21667..22851
                     /gene="mobT"
                     /locus_tag="ACG9CY_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4937837.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            22848..22985
                     /locus_tag="ACG9CY_001318"
     CDS             22848..22985
                     /locus_tag="ACG9CY_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4937838.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7644 a         4091 c         4955 g         6319 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    12961 cggaaaagct gctcgtatca aagaaatcag aagataaaca cggaaaactt ccttggaaat
    13021 ctgaaatttg tgaaaagtcc ttgtatatca agggcttttt cttttttctc tatcacagtt
    13081 ttaagccatt tttggctgtt ttggaccatt tttcaatcat attcccttaa aaattgtcct
    13141 agattgtccg acattgacta tcagtgtcgt caaaataggg caaaatttag tagtcagctt
    13201 gacgacaaat ataatacgaa aaataccaaa acatttaccg aatttagcaa cttttgaacc
    13261 caagtttctc aaagggtcag ctattagcaa aaaagacaaa acatgtactt ttctactaaa
    13321 aagttgatgt gataggcttc tatctaaata actattagga gtttttatcg tgaagcaaaa
    13381 tgatggacgt atcatttgga aaaatcaaag tgagttaaaa ctgattttaa caattaatga
    13441 attcatcgaa aaacacggaa ttacatctag ccgtcaatac cagaaaaagc tatcagaaaa
    13501 tccaaattct gcaccaagta tgtggttcat taacaaaaaa tatggatcat gggagaattt
    13561 attgattagt ataggacgag aaaatactgg gtatggaaaa tgggctagaa tgtcagaaca
    13621 agaattattg gaaatagtag aagcctttat taagtgcgag aagataacct ctcaacgaat
    13681 gtacgaacaa aaatcagtag ggaagaatat tccttcactt agtaccatta aaaagatgtt
    13741 aggagatata cgtccacttt ttaaagaaaa aaatgatgga tctcgattta ctgatttcga
    13801 actgctgtta gaactaaaaa atgaaataat ccgattaaaa ctacaggatg atttatcaat
    13861 gacaaagttt cgaaaactag ttcaatcccc aaaactacca tcagttgata caattatgaa
    13921 aagaacgaac aaaaattggg aggaattgat ggcagagatt ggatttgatt atcgaagaat
    13981 caaaatttat aagcagagaa acaacctatc caagacaaag aaaactaaat agtatcactc
    14041 taaatcagac gttcaaaatt aaaatttgga cgtctttttt attacccaaa aacgaaagga
    14101 gatttatttt atgaaaaagc caagatttaa gaattggcgt ttgctcgcaa cactgagtct
    14161 attatgccaa acaattggtg gatcgcttgg cccaacgatt gcctttgctg atgaaattac
    14221 tcatccacaa acggtaactg tggaattgga tttggctcac cagtatgctg tcgaaggtac
    14281 atttagcgat ggtcgtccca tgtcagaagt taccgttccg cactatgcag tttacaatgg
    14341 tgtgaagcaa gatattttct gtattgagcc aggggtgccg atttacaatg agttcactcc
    14401 tggttatgag aaaaatccat tgcctgatat gtcggagaaa gcgaaattag tttccgtttt
    14461 atggaaggaa gctggtacgg atgtggatac acatattgtg gcccagaaga tgatttggca
    14521 agaagttaat ggctacacgc ttcactcaat taaacgttct gatggtagtg cagtaaatat
    14581 cgcagccatt gagtcaaaaa tcaatcaagc cattgcggat tatcaaaaga aaccaagctt
    14641 ccataactcc acagtgaaaa ccgtgttagg tcaatcgact actgtgacag atacgaacgg
    14701 cttgaattta tcggagtttg atgaagttgt cgagaatatc gcaaatattg attatcgtgt
    14761 gaacggcaat caactagtga ttactccaaa tgccaattca aaagagagtg gtgaattaac
    14821 cctaaagaag tcagctaata caggaacacc ggtagcttat aaaatggctg gtcaacaaac
    14881 tttgatggcg ggggcaattg ataagcccaa tacctacaca atcaaaattg atgtggaaac
    14941 tgagggttct ttgaagatta aaaaagtcga taaagaaaca ggaaatgttg taccgggaac
    15001 cgttttccat ttagactttg gaaaaacatt acctgcaaaa gacgtgacca ccgataaaga
    15061 aggcattgcg acattggatg agattcctca tggcgcgaaa gtaaccatta ctgaaaaatc
    15121 agtgccagct ccttatacga ttgacactac accaatcacc gcaaccatta aatctggtga
    15181 gactatttct gtgacttcaa aaaatactcg cgaaaaaggt caaatcattt tgaataaaat
    15241 gggggtggaa acaggaactg atctttggaa cgacaaatac tcattagccg gaaatacgtt
    15301 tgccattcgc aaagacagtc caaccggtga aattgtccaa gaaatgacca cggatgaaaa
    15361 aggtcatgcg gaaacaccaa aagagattgc caatgcgttg gaattgggaa cctattatgt
    15421 gaccgaaacc aaagctagta atggctttgt gaataccttt aaaccagtaa aagtcgaatt
    15481 gaactatgtc aatcaaaccg tggctcttgt taccagtaac gtaaaagggc aaaaccaaga
    15541 aatcactggt gaaaccactt tgacaaaaga agacaaagat accggtaatg aaactcaagg
    15601 taaagctgag tttaaaggag ctgaatatac tctctttact gcaaaagatg gtcaagcggt
    15661 caaatggagt gaggctttta aaccagagtt agtgaaggga acgaaagctt ctgatgaaac
    15721 ggtgactttg actttagatg aaaagaatca agttgctgtt aaacacttag cgattaatga
    15781 gtattactgg caagaaacca aagcacctga aggatatacc ttggatgaaa caaagtaccc
    15841 tgtttccatc aaaaaggtgg atgataacga aaaaaatgcc gtgattaccc gagatgttac
    15901 ggcaaaagaa caagtcattc gctttggctt tgatttcttt aaatttgctg gatcagctgc
    15961 tgggactgcc gaaactggct tcaatgatct gacctttaaa gtatcgccat tggaaggaac
    16021 cagtgagatt acaggtgccg aagatgaagc aattacagct tataacgagc aactaggttt
    16081 tgatggctat ggcaagtttg aaaatcttcc ttatggggat tatttacttg aagaagtaga
    16141 ggctccagaa ggatttcaaa aaattacgcc attagaaatc cgttctactt ttaaagaaaa
    16201 taaagcggat tacactaaga gtgaatacat ttttacgatt accgaaaaag gacaaaaaca
    16261 accaattaaa acggtgaccg ttccttacga gaaactgacg aataaagcat tttctgttag
    16321 cttgaatcgt ttgatgctct atgatttacc tgaagaagaa gatagcttaa cttctcttgg
    16381 gacttggaaa gacggaggca aagaattgac agaccttgat tctaccgagc tagttgataa
    16441 attgagctat aacttgcatg aaatcaaaga agactggtat gtggtagcgc aagccattga
    16501 tgtggaagcg acaaaagctg cccaagaaaa agatgaaaaa gccaaaccag tggtaattgc
    16561 cgaaacaacc gcaacgttgg cgaataaaga gaaaacagga acttggaaaa ttttgcataa
    16621 attaaccgca gaacaagttt tggataaaac catcgtctta ttcaactatg tgtatgaaaa
    16681 caagaaaccc tatgaagcag gtgatgatcc agtagcgaaa gatgctagct tgaacaatca
    16741 agcccaaact gtcaattgta cggtggaacg ccatgtttcc atccaaacaa aagcccacct
    16801 agaagatggt tcgcaaacct ttactcatgg ggatgtggtg gatatgttcg atgatgtgtc
    16861 gattacccat gatgtactgg atggctcaaa agaagctttt gaaacaattc tgtatgcatt
    16921 acttccggat aatacgacca aagaaatttg gaaatctggc aaaattgatt atgaagtgaa
    16981 tgacaaagaa ttcactaaaa ccgtacttgc ggaaaaagtg gacacaagta aatatccaga
    17041 aggtacaagt tttaccttca aagaaattaa ctatggcaaa gacggaaaca tcaatgggaa
    17101 acacaatgag gatctaaaag aaaaaactca aactttaaca cctaaaaaag ttccgacaac
    17161 accaagtgca ccagaacaac cggaaatacc aactgttcca agtgactctc aagaatctag
    17221 tcctacagta aagacattcc cacaaacagg ggagaaaaat tctaatgttc tgctgtttat
    17281 tgggtttacc ttgatctttg cgactgctgg gtattatttc tggaatcgcc gaaactaagg
    17341 tgatgtgatg aaacaactaa ataggaaaaa ccaacgtgcg cgaccgcctc ctaaaaaaag
    17401 tggtcgcgct atttttgtat ccaaaactaa aaatgaaaag aggaaaagaa gatggaattg
    17461 aaatttgttg tgccagatat ggccgaaacc tttggaaaga taaggtatgc cggcgaaggc
    17521 gaagttttaa ctgaaggata tgggcggaat actacagtaa ttggtcgtag ttatcatttg
    17581 tattccagta agcaacgagc cgatgatatt gaagtggtgg tagctgcgga agccggcgaa
    17641 aaggattttg accaagatca accactaaaa gccgtgaatc cccatttggt tgccaaaggc
    17701 tatgagattg agaatcgtgg gtttaccgac tacgtgttgt atgtcgatga tttagtgaaa
    17761 gcataggagg aaaagaagat gagattaaca gaaggtattg ttgtagattc aggactaacg
    17821 tttgggaaat tacgattttc cgcattacgc cgagaagtac ggaagcaaaa tgaggatgga
    17881 acggttagta acgaggtaaa agaacggact tataatttga aatcctctgc gcaaggtcga
    17941 atgatccaag ttagtatccc agcaaatgta cccttacgcg agtttgccta tgacgcagag
    18001 gtagagttgg tcaatccaat catggatacg gtcgccaatt atgttttccg agaaggaact
    18061 actgtcaatt ggttcattaa ggccgatgat ttagtcttga aacgacaacc agatcaggga
    18121 aattctacaa accaaaatga aggaaagaaa taggtgaagc gtatggaaat taatgttgaa
    18181 catattttag attgtctcga tcaatatgga aaaggtgagc taacggagga acaactcact
    18241 aaagcattaa cctatgatga aaaaatgttt ctgattatgc atcaagggtt actggaagta
    18301 ggtaacgatg tcgaagaaga ttttgatgta ctgaattggt taggggaaga agagtccttc
    18361 tttatggtta tcgaagtaaa tgaaagtcta tgtcgagaag ccgaaaccgt attggaagaa
    18421 attggtgtgg aaatgccgga tgccattgaa gtttttttaa agcaactagt ggaaacgaaa
    18481 caactcccta tagcagtaaa tgactagcat aactgcttga aattggatca atgaaatcct
    18541 atgaagcttt tttcgattgg aggtggaaac cataaaaatg tacaaagggc atcgtatacg
    18601 ggcaggagat cagcacttgg tttatcactt tgttcttggc tggctactcg ctctgttcat
    18661 cggttggatg agcgtctttt attttcaaga gcttagacaa tttgatattt ctaagctatc
    18721 actttctact atagaaatcg ttcgttctat aaaagattta atctatttgc taggaagtct
    18781 cgttctttca ggaagcacga tgttgcttta tatacacttt tttcaggatc attggcgcag
    18841 tttgtggcat cgacaaaagc tagcccggat gattctagaa aatcattggt acgaagtaaa
    18901 acaaactcag agtgaaggat ttttcaaaga cctcaatagt agtcgaacca aagagacaat
    18961 cagttacttc cccaaaatct attaccgtat gaaggatggt ttactttcta ttcgcgttca
    19021 aatttcactg ggaaaatatc aagatcagct tttaaagctg gaaaagaagt tagaaagtgg
    19081 tctatattgt gaactagtag aaaaggaact caaagactcc tatgtggagt acactttact
    19141 gtatgatata attgccaatc gaattgggat agacgaagta gtggcagaaa gtggggcttt
    19201 gcgattgatg aaaaatcaag tatgggctta tgattcttta cctcatatgt taatcgctgg
    19261 cggcacaggt ggtgggaaga cttacttttt actcactatc atcgaggctt tattaaaatc
    19321 tgatgccgag ctttttgttc ttgatcccaa aaatgcggac ttagccgatt taggtacggt
    19381 aatgcctcat gtctattccc aaaaggagga aatttctgct tgtgtggaag atttctatga
    19441 gcgcatgatg actcgtagca aggcaatgaa agaaatgccc aactacaaaa caggagagaa
    19501 ttatgcgttt cttggacttc caccaaactt tttaatcttt gatgaatacg tggtttatat
    19561 ggaaatgtta acgacgaagg aaagtgcggt gattttgaat aagctcaaac aaatcgtgat
    19621 gttaggtcgt caatctggtt tctttctgat tctagcttgt caaagaccgg atgcgaaata
    19681 tttaggagac gggattcgcg atcaatttaa tttccgggtg gctttgggtc gtatgagtga
    19741 actgggatat tccatgatgt ttggcgaggt tgataaaaaa ttctttatga agcgtatcaa
    19801 aggtcgaggc tatgtagata cgggagggag tgtgattagt gagttttata cgccacttgt
    19861 accaaaagga tatgatttct taagggaaat tagcgacatt ggtggattaa acgttaatac
    19921 ggaaggataa aataatagta tgtagcctag gagtgtcaag aatcctgggc tttttttacg
    19981 aggtgattta tgacattttt agatttattc gctgggattg gtggcttttg cttaggtatg
    20041 gaacaagcgg gtcaccagtg tatcggcttt tgtgaaattg atgattttgc tcgccagagt
    20101 tataaggcaa tccatgatac aagtaaggag gtggaaatgc atgacatcac aagtgtatca
    20161 gacgagttta ttcaatctct cggaccagtg gatatccttt gcggtggatt tccgtgccaa
    20221 gctttttcaa ttgcgggaaa gcggcaagga ttttccgata ctcgaggtac tttattcttt
    20281 gaaatcgctc ggttcgccgc tattctccaa cctaagtttt tattccttga gaacgtccgg
    20341 ggattactca atcacgaagg aggggctacg ttcgagacga tcctccgaac gctggatgga
    20401 ttggggtatg atgtggaatg gcaagtgctt aactcaaagg cctacgttcc ccaaaaccgt
    20461 gagcggatct tccttatcgg acattctcga gacgcatgta ccgaacaagt atttcctatc
    20521 attggatcgt ctccaacatc tgatcaaaac atcagaaact tattaaatat caacccttcc
    20581 aatcgaggaa tggggggaca agtctatggg tcagatgacg tagcgcctac cttgaccggt
    20641 gatgaaggaa tcaaaatcgc attgccggta gatgatggaa tttctgtagc gggtatgtta
    20701 ctgggaaatt ttgaacaagg caatcgggtt tatgagataa ctggaacagc gcctactttg
    20761 tcaacgaaac aaggcggaac gaagatcatg cttcggaaaa acgaaacaga atatcaagag
    20821 gtgaagccag ggaacagtgt gaacctggct tttcctaatt ccacaagtcg tcgaggccgt
    20881 ctgggaaaac aaagtgtgca taccttatta acaggagacc aacaagcagt cgtcacggat
    20941 cagtatcaaa ttcgaaagtt aacacctagg gagtgctggc ggcttcaggg ctttccggac
    21001 tgggcttttg atcgagcttc tcaagtcaat tcagatagtc agttgtataa gcaggctgga
    21061 aattctgtaa cagttccggt catttttgat attgctagaa gattaaagga ggtaaaaaca
    21121 gatgatttat gatgagttga ttggggaatc tactgggtga ttggcaagat tcagtcagat
    21181 ccggaattgg aagaagaact gtaccggctc aattttgata ttcgcaaaaa cggggtgaaa
    21241 gtccctgatg atccgtatgt aattgatgag gaaacggatg ctcgaatgga gctgaatcaa
    21301 gtcatcagtg aattggaacg aattgcggac tttgctaaag aacctgatat ccgccaatac
    21361 ttgtttgaga taaaagcaga gttggaaatt gagggtatta ccgcggagta acggccaaat
    21421 aaaccactga taggcactga ccgcggggga tttgcgaagc agaaatcctc cgtggtgctg
    21481 tgcctgttta tggacggcgt tagccggcca tgatgtctca ccccccgtat ctaacagggg
    21541 ggtacaaaaa cacaaaaaat aagcaagcaa cgataaggaa tggctgtcct atcaaggata
    21601 ataagcaatt tgtgtagatg ttaactgatt tgaaaaagtt aacaccttaa atttggaggt
    21661 gagaacttgg cacaaaggaa tttagattat cggctattaa aagaccggcg taatgaatat
    21721 ggcgtttcac aaaataaatt agctacgact tgtggactta gtcgcccgta cttaaatcag
    21781 atagaaaatg gtggcgtaac cgcatcgaca aaaaccatga ggaaaatttt tgaccaactg
    21841 gaaagtttca atcccgagtt acctttaacg ttactgtttg actatgtaag gattcgcttt
    21901 cccacaacgg atgcgaggaa aatcattcaa gagattctcc atttgaaatt tgattatatg
    21961 ctccacgaag attacgcctt ttactcctat caagaacaat atgttatggg agatattgtg
    22021 gtgatgttgt cacatgaaga agataaaggc gtccttttag aattaaaggg tcgtggttgt
    22081 cggcagtttg aaactttttt actcgcccaa aagcgtagct ggtatgactt tttcgaagat
    22141 tgtctaaaag ccggtggcgt gatgaaacgc ttagacctgg caatcaatga tcgagttggc
    22201 ctgttagata ttcctgattt aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt
    22261 cgtaccttca aaagctatcg ttccggggaa ctgttgaaac ctgatgataa agatggtatg
    22321 ggcaatactt tgtatattgg cagtcttaag agtgaggtct atttctgttt gtacgaaaaa
    22381 gactatgagc aatatatcaa gttagggatt cctttagacc agaccgaaac gaaaaatcgt
    22441 tttgaaatcc gactgaaaaa tgatcgagct tatcatgcaa ttcaagattt gttaaaaggt
    22501 cgcagtattg aaagtaccac attttccatt attaaccggt acttgcgatt tgcggataag
    22561 gtggaaggca aaaggcgaac taactggccc ttgaatgaac aatgggggcg cttcattgga
    22621 cggaatcaga aggaaattca attaacttct gaaccgaagc cttataccat tgaacgaacg
    22681 ttgaactggc ttggacgaca agtcgcccct acgtggaaga tggccaagga gttggatcgg
    22741 ttgaatcaga caacgtacat tcaggatatg gtaaataata ctcgactatc agaccggcac
    22801 aaaaagattt tggagcaaca aagtatggca attgaaaatt tgattatatg aaaagaggaa
    22861 tcgtgatgga aattgtgaaa gatattttat tggtgtttgg tggcagtatg cttggtgtta
    22921 ctgtgatgtg cttgatgcat gcaagtgcag cggcggaccg agcaatggaa aagaaaggaa
    22981 agtgatgaaa tgaatttcgg tcaaaattt
//
LOCUS       DAZULG010000039        22852 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000039 DAZULG010000000
VERSION     DAZULG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.039"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACG9CY_001319"
     CDS             3..371
                     /locus_tag="ACG9CY_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS4937839.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACG9CY_001320"
     CDS             551..1492
                     /locus_tag="ACG9CY_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS4937840.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACG9CY_001321"
     CDS             1561..2292
                     /locus_tag="ACG9CY_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4937841.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACG9CY_001322"
     CDS             2436..4616
                     /locus_tag="ACG9CY_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS4937842.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACG9CY_001323"
     CDS             4636..5118
                     /locus_tag="ACG9CY_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS4937843.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACG9CY_001324"
     CDS             5134..5895
                     /locus_tag="ACG9CY_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4937844.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACG9CY_001325"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACG9CY_001325"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS4937845.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACG9CY_001326"
     CDS             7667..8152
                     /locus_tag="ACG9CY_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS4937846.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACG9CY_001327"
     CDS             8145..8828
                     /locus_tag="ACG9CY_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS4937847.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACG9CY_001328"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACG9CY_001328"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS4937848.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACG9CY_001329"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACG9CY_001329"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS4937849.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACG9CY_001330"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACG9CY_001330"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS4937850.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACG9CY_001331"
     CDS             13125..13457
                     /locus_tag="ACG9CY_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4937851.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG9CY_001332"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG9CY_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS4937852.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACG9CY_001333"
     CDS             14283..15656
                     /locus_tag="ACG9CY_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4937853.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG9CY_001334"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG9CY_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS4937854.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACG9CY_001335"
     CDS             16711..17151
                     /locus_tag="ACG9CY_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4937855.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACG9CY_001336"
     CDS             17295..17996
                     /locus_tag="ACG9CY_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS4937856.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACG9CY_001337"
     CDS             17993..19114
                     /locus_tag="ACG9CY_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS4937857.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACG9CY_001338"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACG9CY_001338"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS4937858.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACG9CY_001339"
     CDS             20493..21401
                     /locus_tag="ACG9CY_001339"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4937859.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACG9CY_001340"
     CDS             21436..21708
                     /locus_tag="ACG9CY_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS4937860.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACG9CY_001341"
     CDS             21719..22765
                     /locus_tag="ACG9CY_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS4937861.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7699 a         3983 c         5015 g         6155 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataatattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt at
//
LOCUS       DAZULG010000040        22688 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000040 DAZULG010000000
VERSION     DAZULG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.040"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..383
                     /locus_tag="ACG9CY_001342"
     CDS             3..383
                     /locus_tag="ACG9CY_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS4937862.1"
                     /translation="MFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEMHLVDPEKIGA
                     TGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVAAGKTAEGVEA
                     LLGYVRKKKAERVSVNEMEENQID"
     gene            376..1077
                     /locus_tag="ACG9CY_001343"
     CDS             376..1077
                     /locus_tag="ACG9CY_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS4937863.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1077..2663
                     /locus_tag="ACG9CY_001344"
     CDS             1077..2663
                     /locus_tag="ACG9CY_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4937864.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2682..2903
                     /locus_tag="ACG9CY_001345"
     CDS             2682..2903
                     /locus_tag="ACG9CY_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS4937865.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2900..3661
                     /locus_tag="ACG9CY_001346"
     CDS             2900..3661
                     /locus_tag="ACG9CY_001346"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS4937866.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4041..4550
                     /locus_tag="ACG9CY_001347"
     CDS             4041..4550
                     /locus_tag="ACG9CY_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4937867.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4625..5164)
                     /locus_tag="ACG9CY_001348"
     CDS             complement(4625..5164)
                     /locus_tag="ACG9CY_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS4937868.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5304..5915)
                     /gene="rpsD"
                     /locus_tag="ACG9CY_001349"
     CDS             complement(5304..5915)
                     /gene="rpsD"
                     /locus_tag="ACG9CY_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS4937869.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6177..7028
                     /locus_tag="ACG9CY_001350"
     CDS             6177..7028
                     /locus_tag="ACG9CY_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4937870.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            7056..7691
                     /locus_tag="ACG9CY_001351"
     CDS             7056..7691
                     /locus_tag="ACG9CY_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS4937871.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7711..8484
                     /locus_tag="ACG9CY_001352"
     CDS             7711..8484
                     /locus_tag="ACG9CY_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4937872.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8892..9689
                     /locus_tag="ACG9CY_001353"
     CDS             8892..9689
                     /locus_tag="ACG9CY_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS4937873.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9667..10080
                     /locus_tag="ACG9CY_001354"
     CDS             9667..10080
                     /locus_tag="ACG9CY_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4937874.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10064..12709
                     /gene="mutS"
                     /locus_tag="ACG9CY_001355"
     CDS             10064..12709
                     /gene="mutS"
                     /locus_tag="ACG9CY_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4937875.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12727..14835
                     /gene="mutL"
                     /locus_tag="ACG9CY_001356"
     CDS             12727..14835
                     /gene="mutL"
                     /locus_tag="ACG9CY_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS4937876.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14857..15417
                     /locus_tag="ACG9CY_001357"
     CDS             14857..15417
                     /locus_tag="ACG9CY_001357"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS4937877.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15502..17490)
                     /gene="treP"
                     /locus_tag="ACG9CY_001358"
     CDS             complement(15502..17490)
                     /gene="treP"
                     /locus_tag="ACG9CY_001358"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS4937878.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17713..18426
                     /gene="treR"
                     /locus_tag="ACG9CY_001359"
     CDS             17713..18426
                     /gene="treR"
                     /locus_tag="ACG9CY_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS4937879.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18503..18949
                     /gene="msrB"
                     /locus_tag="ACG9CY_001360"
     CDS             18503..18949
                     /gene="msrB"
                     /locus_tag="ACG9CY_001360"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS4937880.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19120..19722
                     /gene="ruvA"
                     /locus_tag="ACG9CY_001361"
     CDS             19120..19722
                     /gene="ruvA"
                     /locus_tag="ACG9CY_001361"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS4937881.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19735..20736
                     /gene="ruvB"
                     /locus_tag="ACG9CY_001362"
     CDS             19735..20736
                     /gene="ruvB"
                     /locus_tag="ACG9CY_001362"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS4937882.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20765..21343
                     /locus_tag="ACG9CY_001363"
     CDS             20765..21343
                     /locus_tag="ACG9CY_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4937883.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21395..21877
                     /locus_tag="ACG9CY_001364"
     CDS             21395..21877
                     /locus_tag="ACG9CY_001364"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS4937884.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21994..22368
                     /locus_tag="ACG9CY_001365"
     CDS             21994..22368
                     /locus_tag="ACG9CY_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937885.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(22481..>22688)
                     /locus_tag="ACG9CY_001366"
     CDS             complement(22481..>22688)
                     /locus_tag="ACG9CY_001366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937886.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7380 a         4066 c         4910 g         6332 t
ORIGIN      
        1 ttattttctt ttctgtatta ggagaaggca tacggatttt tgctcattta cctattcctg
       61 gaagtatctt aggtattctt tttttatttt tagcatttga aatgcatctt gttgatcctg
      121 aaaaaatcgg agcaactggt gattttttat tgaataatct agccatttta tttgttcctg
      181 ctggtgtagg tttattggaa tattttgatg atattgcttc gatatggcct gttttactgg
      241 gagcagtcgt cgtgtgttcc atcgtcacga tggttgcagc gggaaaaacg gcagaaggtg
      301 tagaagcttt gctgggctat gtccgtaaga aaaaagcaga aagggtatct gtaaacgaaa
      361 tggaggagaa tcaaattgac tgagtttttt gtgaatccat tatttggctt aagtttgact
      421 ttaggagtgt acgtattatc tgacaaattt ttgcggaaat ataaactgcc tgttttgaat
      481 cctctcgtat tatcgattgt tgttattatc ttatttttgc atttttctgg gatcagctat
      541 aaagactata atatcggggg caattttttg actatgctga ttactcctgc gacagtggca
      601 cttgccatcc ctctttataa aaacttccgt ttactgaaag agaattttta tcctgtaatg
      661 gctgcgattt tagcaggaat agtcgcaaat tgcttggtta gtgtagggtt cggttacttc
      721 ttctctcttc ataaagagat ggtgatctca ttactaccta aatctgtaac gacggctatt
      781 tctgtcgatt tatctcatac gatgggcggg gtgaatgccg tgacacttgc tattgttgtc
      841 tctacaggta tttttggttc gctgatcgcc actcatattt tccgcctatt caagatcgaa
      901 agtccagttg ctcgcggtgt ggcgttagga agtacttctc atgctattgg tacagcaaaa
      961 gcaatcgaga ttggtgaaat agaaggaatc atttccggac ttgcgatatg cgtcaacgga
     1021 atattgaccg ttttattgct accgttattc tttcagcctt ttgctggctt gttttgatgc
     1081 gaaaaccact attttatgct acaataacca aaacgttcga gcataaaaaa ggagggaaag
     1141 acgtgtattt gtcttccgag tatcttaaac aatttgataa agatctatat tataagattc
     1201 tcctcctaga aagttttgag gatcaagctt ggcataccgc tgcacaatta gcgcaagttg
     1261 ttcagctgga tgcccgcagc gtgtcgaaat acctgaacga gctgtctaaa aactatcagc
     1321 agtttagcgg taagacacac cctttgttta caaaaaatca ccgttctggt tataactttt
     1381 atgatacgct cgattcaatt gaacatgagc gttttttgat ctatctcgtg caaagtacgc
     1441 tgaaattcca gttgctgcac gacatttttt ttgaagaatt ccatacaatg taccaatttg
     1501 cgcaaaagca ttacattagt gaatctactg ctcatcgaaa aatcaatgaa tggaaacagc
     1561 aactgcaaac ttacggaatt cggctgcaac gaggtacata tatcgctcaa ggcgaagaag
     1621 aaatcattcg actttacttg catatgacat tctggcagtt gtttcgaggg aaaatctggc
     1681 catttgaaac gatttctcaa atggacgtaa agaatatggc agaacatatc atggcttttt
     1741 ttaatgttcg tctgaacgag atcaaaaaaa gacgattgga atatatgcta ggtgcctttt
     1801 ttttaagaaa atcacagaag cattatgttg tattgaatga gaaaaaaaga cggctgattt
     1861 ctgacaatct gttgttccag cgattttgcc aggtcatgga gccggtattt cctaattatt
     1921 ttcaagtaga agacgagtta ggcgcattat ttcttgtact gatgactcgt gaagaatatt
     1981 atagtgatcc taagatacgg aagaaaattt ttgattttca tcaacaagca aaaacaccgc
     2041 cctttacagc cctttcagaa gcaaaagccg ctctttctct ttatcaggaa gagcaggggc
     2101 tgccagcaga aaatctaacc ttcgaggcag aaaactattt attcagcagt cattttttcg
     2161 cctatctttt tcctaatgcg aaagaaacga ttgatggcaa tagtagtgat tttattaatc
     2221 atttagttat tgaaaacaag gaattgaaac aatggctcgt acattttttt gaaagtagac
     2281 acaaacatcc caatcatctg gcatttaaaa atcatgcgtt tttgatgggg cggtatctga
     2341 ctgtgtttaa aacattagga gcctttactc cacagcttcc aaagatcacg atccttttaa
     2401 tgacagattt cccattattt gaagagcaac tgttagagga gggattacgg acatttttcc
     2461 gtaatgagta tcagctgatt tttctgccaa cagattatcg ggggagagaa gttgatcttc
     2521 tgatttcgac aagtaaagtc caccgcaaac catgggcgga tcttgactat tttattgtga
     2581 ctgaagaact aaaattaatt gattacatcc aactatctca aaaatttgaa atgattcaaa
     2641 aacaaaaact attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt
     2701 taccagaatt gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt
     2761 atgttgttct cgtatccgat gatgaatttg atggctgtcc ggtgacgccg tacttattga
     2821 tcaaaacaag tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag
     2881 atgattgggc gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt
     2941 tatcacagga gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga
     3001 tgcttttgta tttggattag atcaaaagtt cggtaaaatg gaagaaattt ctttgcgatt
     3061 tcctgaaact tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc
     3121 ggtatcgaaa tgccaaaatc attttggtga agttacgatt ttactgaata cagcggggat
     3181 attggatgcc tacaaaccct tgatggaaac tgacgaatcg ctgtgggatc tgatttatga
     3241 gacgaatgtg aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgcca
     3301 aaaaagcgga acgatcatca atatggcttc gattgctggc ttgattgctg gtggaggtgg
     3361 gatcgcttat acaagcgcga aacatgcgat tgtcggattt accaaacaac tcgctctgga
     3421 ttacgcagga cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat
     3481 gaatgcggct gattttgaag ggaaaggtga gatggcagag tgggtggcaa atgaaacacc
     3541 agtcaagcga tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc
     3601 tcaagcaagc tacatacaag gaacgatcat cccaatcgac ggcggatggt tgttaaaata
     3661 accttgtgtt tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg
     3721 ataaaaaaag tcgatgactg ttcccgacat tcgatgccga acagccagtt caaaaaattc
     3781 tttttaataa tcgttaatat tcgaaactaa gcggcttttc acgacctctt gatataaacg
     3841 atattcaaaa gcttgcaact gcggtaacaa gtccagccaa atgaaaaata taaggagcta
     3901 tttttatttg accattctta ttacttgttg cagggcagct ttttcactac cttctgcgac
     3961 acttcagttg gaattttctt cccatccttt ggtcgcggtc attaccaaga taaaattatc
     4021 ttgcaaagga gaaatagaac atggaaaaaa ataatacccg ttcagccaaa cgctggacaa
     4081 cgacagatgt gactcggatg gcgctagtca ctggtttata tgtggcagtg acgctggttt
     4141 tgtctgtctt tagctttgga gtgattcaga ttcgtctctc ggagatgttc aactatctgt
     4201 ccttgtataa caaaagatac atcgtcgcgg taacagcggg agtagctttg gctaatattg
     4261 cttctcctct tggactgata gatgttgcag taggaagcgt cagcacgctg cttgtattgc
     4321 ttatcaacta taaaatcact agtcgcatca aaaacatgaa aatcaaaatg gccgtgacag
     4381 catgcgtatt tgctttttcc atgtttacag tagctggtca gctaacgatt ttatatcaac
     4441 tacctttttt cttgaattgg tgggtgattg ctttaggaga attggcatca atgattgtcg
     4501 gtgggataat tatttattgg atcggtaaaa aagtggattt gtcaaaataa agatctgttt
     4561 aatttaaaat agggcagcat ggaagagact gaatgttgag tcttcttcca tgctgctttt
     4621 ttgattaatt tgtatgaaga aatcgattta gccgtttttt catcaagata ccgatataag
     4681 ctgctccagt tgccttgatt gcttccggca gtaaaaacgg cagtagtcca ctggcaaatg
     4741 cagttgtaaa agtaagatca ccacttattt tcagccagat cgttccgcag atcaatgcag
     4801 caagagagcc cattagattg gcaaagattg catataagta atgagcatcc gttctttcga
     4861 tcatccaacc agttatccac gtttgtaaga gaaatccaat caaatatccg ccagttggcc
     4921 caaataaaac agctatacct ccagtcatcc ctgaaaatac aggaagacca atcaagccaa
     4981 gtaaaagata aactccaaca gatatcaatc ctagttttct tccgagaaaa gttgctgtta
     5041 atccaatagc aaatgtttgc cctgtcaaag gaaccaaacc aaaaggaatg gtcatctgag
     5101 aaaggacaga aataatcgcg gcaaattccg cagcaagtat caattctttc aatgttcggt
     5161 tcataacgcc ctcttttcgt taacttaata tttttatttg gttaacgaaa ttgtaaaaca
     5221 aagtgattac tttgtaaata gaaaatataa aaaaagacca gctatccaat agatagcggt
     5281 ctttttcaag aaagcttttg aaattataag ctttggttgt agtattcaac gacaagagct
     5341 tcgtcgattt ctggatatag ttcgtcacgt tctggtaaac gagtcaatga accttccaat
     5401 ttttcagcgt caaagcttac gaatgctggg cgtccaacag ttgcttctac agcttctttt
     5461 actgttacga tgttttgtga tttttcacga actgagatca cttgacccac ttctacgtgg
     5521 tatgaaggga tatcaacgcg tttgccatca actaaaatgt gaccatggtt aactaattga
     5581 cgtgcttgac gacgagtcgt tgcaagtcct aaacggtaaa caacattgtc taaacgttgt
     5641 tctagtaaga tcatgaagtt aacaccatgt ttaccttctt tgattttgct agcttttacg
     5701 aacaagttac ggaattgacg ttcgttcatt ccgtacatgt gacgaagttt ttgtttttca
     5761 gttaattgca tgccgtattc tgattgtttg ccacggctat taggtccatg ttgacctggt
     5821 gcgtaagggc gacgtgctaa ttcttttcct gtgcctgaca atgagatgcc taagcgacga
     5881 gaaattttcc atgatggtcc tgtataacga gacattaaaa tttcctccaa tgaatataag
     5941 tttttggagt aaaataatcc gttgaaaaat tcataatcat atagtttgtt cttcaatctt
     6001 cacctttgca gccgcggtta cacaatcgaa cccgagacgg ggcaacaaac tagggatgag
     6061 ttattatttt tctgctgcat tattttacac aaattacagt atatgataga tcatccacga
     6121 atgtcaagat aattagtaca taaaaacgct ttacaaacct aggctgtctt tataaaatga
     6181 gagggtatgt gcaaatggaa aaaagcgaag gaagtttaag gagaaaatca atgaatccgg
     6241 tatcaccatt atttgagaaa atcgatgctt caattgaaaa gaaggcgaat ctttttgaaa
     6301 acagcttttc acgttacgct gttcgagcgg ttctcgcctg tttgtttttg actttaggaa
     6361 cggctgtggc atttgcgatc gcgatgaagg gggaagatat cgcgcatggt ctaggaaaga
     6421 ttttatacgc atttatgttc agctggggtc tggtcatgat tctatacatg aacgccgaat
     6481 taggaacatc gaatatgctt tatatgaccg tgggtgtttt tcggcagaga cttgctttaa
     6541 gtaaggcagc caagattcta tttgcctgta tttttttcaa tatgattggc ggtatattat
     6601 gtggatttct aatttctatg actacgccat ttcaagattt aacgttggac aactttatgt
     6661 tagaatcaat agcagtaaaa ctagcaaaac cttcagcaac gattttggtg gaagggatgt
     6721 ttgcgaatat cgttgtcaat accgctgtac tgatcagcat gcggatgaaa gaggatgtcg
     6781 gaaaagtgat cacagtcgta ttcatcattt tcatttttgc ttttttagga tatgaacacg
     6841 tgatagcgaa ttttcctgcc tttacgctta cttactttgc ttctcacgga cagatggatg
     6901 gaatgacagt cagcaatgtg ttacataatt tgttttttgc tttggcagga aattatattg
     6961 gtggcggttt agtaatggga ctgggatatg cttggctgaa tcagtcaaaa tcatcctatg
     7021 tggactaaaa agtcgttcat taaaggggaa gtgccatgga agttttagtt tttccaaaaa
     7081 atgaggaaaa agcgttacaa atggaaaagt acatgaaaaa tcttttttcc tttgctggag
     7141 tacctaagcc ggaacgcgct cttttacaac aaccgttact ggatgagagc aaaaatttat
     7201 ctatttctga agttttagct tttgttcatt attattatga acaaatcgaa agagagtatc
     7261 agtatgtagc gatcgattta gtccagaaaa acatcaaacg gcttacattt gaagacttag
     7321 tcgtattgtt gccactagtt cagaaaaaag aatggtggga cagcatcgac tcttggagaa
     7381 aagtatacag cacatggtgt aaagcctacc cagagcagcg tgaagaagtc tttcaattgt
     7441 ttgaaaaaaa ggaagatttt tggctacgtc gaatagcaat cacgttgcaa ttaggttata
     7501 aacagcaaac agacacagga ctattgatcc gagcaataga cgaagatcgt caaacaaagg
     7561 aattctttat ccaaaaagca attggttggg cattacgcga ttatagtaag accgatgccg
     7621 attgggtaaa aagacagctg acaacagatt tgagtaagtt agctgttcga gaggcaagta
     7681 agtatttata agaaagcgag acgacaagag atgagacgca aattatttgc ttttgatatt
     7741 gatggaacgt tgttagattc aaacaaacaa gctttagata gcacaagaga agcattggaa
     7801 aaattacgca aagccggtca tctggtcacg attgctacag ggcggagccg atttcatgcg
     7861 caagagatca ttcgcgatct tgattttacg aactacattt tatgtaatgg agcggccggt
     7921 tttttagacc atgaacaagt atataagaat ttactagatc gcgaccagtt ggatgcgttc
     7981 gttcaagaag cgcatcatga gcagatcgat acagcttttg tcagcttaga caatatcaag
     8041 cgttcttctt ccaatgatgt aacggtgatg gaagaagcga tgcattcttt cggtgctgtc
     8101 ttgcctgaat tagatcagtt ttttgcagag gaacaagaaa tataccaagc gttagcattt
     8161 ttcgatcaaa catatgatgg gaagttctct tcatatgata aaattcgatt tgtccgctgg
     8221 catgagaaca gtgtagatgt cgtacctaaa aatggttcta aagcagcgac aattctgcac
     8281 ttagcagaac aagtaggcat ccctcatgaa gacatcatca gctttggcga tggacaaaac
     8341 gaccgtgaaa tgttgcgcat gtcaggaatt gggatagcta tggggaatgc tgtgccagaa
     8401 gtacaagcag aagctaagat ggtcactgat acaaacgatc aggatggaat ttggaaggca
     8461 ttgaaagagt taagcgtgat ttaaaagagg ttgtgaaaaa gctgttctgc atcaagtaat
     8521 aagcccagcc aaacaaaaat agctcctcct atttttcgtt tttctgggct tattaccgca
     8581 gatgcaagct ttcgaacacc gtttaaggaa gaggtcgtga cagaagtggt cagtttcaag
     8641 aaataagaag ggatttacga aaattgcttc tcaaattttt gtaaaattac ggcttatttc
     8701 tgaagaagta gatgaaacaa cgtttattcg ggaaatggga ctgtgacaag atgtttgtca
     8761 tagtcccatt tttttatttt atttttcaaa actaagaaat accaggaaag agttcctttt
     8821 gtttgacctt ttctttctta ttgaaaacaa ctataatata gagtgatgtt aaaagagaag
     8881 aggaatttag tgtgcgggta ttgtttattg gtgatgtagt aggttcaatg ggacgagaga
     8941 tgattactga atatcttcct agattgaaga agaagtaccg tccgcaagta acgattgtaa
     9001 atggtgagaa tgccgcttct ggacgtggaa tcacagaaaa aatctataaa aaatttttgc
     9061 aagacggcgt agatgtcgtg acgatgggga atcatacgtg ggacaaccgc ggaatctttg
     9121 agtttgtaaa tgaagctaaa aagatggttc gtccggcaaa ttttccggaa gggacgcctg
     9181 gacaagggat ggtctttgtc aaagtgaacc aagtcgaact ggcagtgatc aacatgcagg
     9241 ctcgttcttt tatggtggac ttggatgatc cttttcgtaa aatgaatgag ctggtagaag
     9301 aagccagaaa acgtacgccg atcatttttg tagattttca tggagaaaca acaagtgaaa
     9361 aacaagcaat gggctggttt ttagatggga aagtttcagc agtggtcggt acacatacac
     9421 acgttcagac aaatgatgcg cgaattttgc caagaggaac agcgtatctg acagacgttg
     9481 ggatgacagg tccctatgat ggtattttgg gtatgcgcag agaacctgta atcgaaaagt
     9541 ttctgactgc attaccaaaa cgttttgaag ttgtggaaca aggccgatcg attttaagcg
     9601 gctgtatctt ggaattggat gatacgacag gtcatgcaaa agaaatccag ctgattcaga
     9661 tcaacgatga tcgaccgttc atggagtaga aagagaggca gttttttgca gcaggagccg
     9721 gaaattcaaa aagaagtaag gaaactgaca aaacttttaa gagagaatga aacgatcatt
     9781 cgctacaaag aacttgaaga aaagatccag caaaaccagt atttagctga gttgagagaa
     9841 aaaatcaaac aagcgcaaaa agatgctgtt cattttgccc actatgacaa accagcagca
     9901 gaaaaagaag caatcaaaca agcggatcaa ttcatgcaag aatttgatca gcatccgctt
     9961 gttgtagctt atagaaaaca gctgttggaa gcagatgatc tgcttcatca tttaacaact
    10021 atgatacaag aagaaatcaa tggacaaata gaggaggaaa aacatgcctc aaaaaactaa
    10081 aaatactcct atgatggaac aatatctaag catcaaggcc caataccaag atgctttttt
    10141 gttctatcgt ttaggagact tttatgaact attttacgaa gatgcgatca aagcgtcaca
    10201 gcttttggaa ttaacgttga cgagcagaaa tcgaaatgca gaagaaccta ttcctatgtg
    10261 cggtgtacct caccatgcag cacaaggata tatcgatgcc ttgattgaaa aaggctataa
    10321 agtagcgatt tgcgagcagg tagaagatcc aaagacgact aaagggatgg tcaagcgcga
    10381 agtgatccag ctagtcactc ctggtacagt catggacagc aaaggattgt cggcaaaaga
    10441 caacaatttt ttaacagctg tagttagtca aggaaatgaa tttggctttg cttatgccga
    10501 tttaagtacg ggagaattga agacggctcg gttgcatgat gaagaagctg ttttaaatga
    10561 agcctctgca ttgcagacaa aagaactcgt attaggcagt gagatcactg atacccttcg
    10621 tgaacagcta agcgggcgtt tagggctagt cttttctcaa caaaatgaga tggaagaaaa
    10681 cgcggaattt agctttttga caagcgaact agtcgatccg ctggaaaaag aagcaaccgg
    10741 caaactattg acttatttat cggtgacaca aaagcggagt ttagctcata tccaaaaagc
    10801 agtagaatac caaccagatc attttttgaa aatggattat tattcaaaat tcaacctaga
    10861 attaagccaa tctattcgga ctgggaaaaa gcacggcact cttctatggt tgttagatga
    10921 aacaaaaaca gcaatgggtg gccgtttact aaaacaatgg ctagaccgtc cgcttatcca
    10981 ggcaaaacaa attaaagcaa gacaagaaat ggtcgcttct ttgctagaca gctattttga
    11041 acgtatcgat ttgcagtcag cattaacgaa agtctatgat ctggaacgcc tagcaggtcg
    11101 agtagctttc ggtaatgtca atgggcgaga cttgatccag ctgaaaactt ctttagaaca
    11161 agtgccaatg atcagagggt tgattgaagg aatcgatcaa ggtcaatggc aaagtctgct
    11221 ttcagaaatg cagccgatgg accatctcgt ccaattgatc gaccaagcta tccaagatga
    11281 tccgccgtta caaataacgg aaggaaacat catcaaagat ggattcaatg agcagctcga
    11341 tatgtaccgt tctgctatga gaaacggaaa gaaatggttg gcagaacttg aagcaaaaga
    11401 gcgtcaagaa acaggaatca agaacttaaa aatcggattt aaccgcgttt tcggttatta
    11461 tatagaaatt acaaaagcaa atcttggaaa tgcagaactg gagaagtatg aacgaaaaca
    11521 aactttagca aatgcagagc gtttcatcac accagaacta aaagaattgg agacacaaat
    11581 cctcgaagca gaagaaaaat ctgttgattt agaatatcag ttgtttttag ctgtgagaga
    11641 agaagtcaaa aaggctatcc agccgctaca agtacttgca aaagcaatca gtgcagtaga
    11701 tgtgctacaa agttttgcaa cgatcagcga acgctatcag tatgttcggc cagaacttgt
    11761 cagtgacaag catcagcttc tgatcaaaga cggacgacat cctgtagtag aaaaagtatt
    11821 aggacatcag gaatatatcc ctaacagcgt agaaatggca gaagacgaaa tgatcttgct
    11881 gattacagga ccgaatatgt ctggtaaaag tacgtatatg cgacagttgg cattaactgt
    11941 tctgatggcg caaatgggct gtttcgttcc ggcaaaacaa gcagttttac ctatttttga
    12001 tcggattttt acccgaattg gtgcaagtga cgacttaatt gctggacaaa gtacttttat
    12061 ggtagagatg atggaagcca atcaagccct gcgttatgct acgccaaaca gtttgatttt
    12121 gtttgacgaa ctagggcgag gaacagcaac ctatgacgga atggcgttgg cccaagcaat
    12181 catcgaatat atccatcgtc atgtacaagc aaaaacactc ttttctactc attatcatga
    12241 actgacggta cttgagaaag aacttccgca gcttaaaaat gtccatgttg gagccgttga
    12301 aaaagacgga gaagttgtct tcttgcataa aatgatggat ggacctgcgg ataaaagcta
    12361 cggcatacat gtggcaaaga tcgcaggatt gccttccaat ctactagaac gagcagcaga
    12421 tatcctgcat acgctagaaa atggcgataa cggacaccat actgttccag ctgaagtaga
    12481 agagaaaact aaaacagcca aacaggagca aataaaacca cctgcagaaa aagtaaatca
    12541 agaccatata caagaccgtg taaaagaaga aaaccaacag ctttctttat tcagtgaatt
    12601 atccgaaaat gaaacagaag tcattgaatc actgaagcaa ataaatttac tagaaatgac
    12661 gccaatggat gcactaaaca cattatatga attacaaaaa caactgtagg aagaaggcgg
    12721 atatcgatgg ccaaaatcca agaattatct gaacgtttag ctaatcagat cgctgcggga
    12781 gaagtagtgg agcgaccagc ttccgttgtg aaagaattag tggaaaatgc aattgacgcc
    12841 ggcagtactc agattgatat cttagttgaa gaagcgggac tgcggacgat ccaagtgatt
    12901 gataacggag aaggcatttt ggcagacgac gtagagaatg ccttcaagcg gcatgcgaca
    12961 agtaagatcc ataatcgcga cgatcttttt cgcatacgaa cgctgggctt tcgaggggaa
    13021 gctttgccaa gtattgcctc tgtttctgaa atgactctgg aaacggcgaa tcaggaagaa
    13081 aaacaaggca cctatctgtc gctgaaaggc ggagaagtta ttgagcatcg accagcacca
    13141 ctgcgtcaag gaacgaagat taccgtcagc aatctgtttt tcaatacgcc agcccgcttg
    13201 aaatacgtga aaacactgca cacagaatta gcgaacatcg gggacatcgt caatcgactg
    13261 gcattgagtc atccatcgat tgcttttcgt ttagtacatg atggaaacaa aatgctggca
    13321 actgctggaa atggcgatct gaaacaaaca attgcgggaa tctacggatt atctaccgca
    13381 aaaaaaatgc tgaaaatcga aggaaaagat ctagattttg agttatttgg ctatgtttca
    13441 ttgcctgagg tgactcgtgc cagtaggaat tacttatcga cgatcatcaa tggacgtttt
    13501 atcaagaact tttccttgaa taaagcaatc gttgcaggtt atgggtcaaa attaatggtc
    13561 ggtcgtttcc ctattgctgt attggaaatc aaaatggacc cgcttttagt agatgtaaat
    13621 gttcatccga cgaagcagga agtccggctg tcgaaggaaa aagaactaac agagctaatt
    13681 agtaacgcca ttcaaaaagc attgtctcaa gaaaatctga ttccaagtgc agcggataat
    13741 ctacggttta agaaaaaagt acctgatcag cctaaaacag aacaaatcga aatcgatttg
    13801 tcctacgctt ttgaagaaga aaaacctaaa aagccaagta gtttgaattt tgatcgacag
    13861 acaggtacct ttttcgtaga agaactaaag aacacatttg ttgacagaat tgttgataag
    13921 tctgtggaaa atgttgaaaa ctattctggt ttttctgaag aaaacaccac ttatccagaa
    13981 aaaacagcga aaacggtgga taagtttggt gaaaatgttg ataactttgt agaaaaaaca
    14041 gtggaaaaat ctgaaaagaa agaatttccc attgtggata acatggagaa aacaaatgaa
    14101 gaacaagcag ataaatccgt agaagaggat gaaaagattt acgaggaaga attgacactc
    14161 catcctgaat ttgatttcac tcaaggaaac gacaaaacat tagaacaagc catcaagaaa
    14221 ttagaaacgg aaaaaccagt acaacgtttt cctcagttgg aatattttgg acagatgcac
    14281 ggaacgtatc tttttgccca aagtagcgat gggttgtaca tcattgatca gcatgctgcc
    14341 caagagcgca ttaagtatga atatttccgg gaaaaaattg gggaagtatc tgatgatctg
    14401 caagaattgc ttgttccatt tgtgttagac tatccaaaca gtgatgcttt gaaattaaaa
    14461 gagcaaaaac acttgctaga agatgcaggg atttatttag aagaatttgg tcaaaacagc
    14521 tttattgtac gggcccatcc tacttggtat cccaatggag aagaagaatc gattattcgg
    14581 gagatgatcg atatgctgct gacaactgga agtgtgagtg taaaaaaatt ccgtgaagca
    14641 acagcaatca tgatgagttg caaacgatcg attaaagcca accattattt gaatgaagca
    14701 caagcacgtg tgctgctgaa ggatctggca cgatgcgaaa atcctttcaa ttgtccccat
    14761 ggccgtccag tattgattca ctttaccaat tcggatatgg aaaggatgtt caaacgaatc
    14821 caagacccgc attaagtcaa agagaagagg gaaaacatga agattgtatt agcatcccaa
    14881 tcaccaagaa gaaaagagtt attggctcat atcgtttcag aattcgagat tgaacctgct
    14941 gatatcgatg aaacgccatt tccagacgaa cagcccgtcg attatgttcg tagaatggct
    15001 gaagaaaaag cacgaactgt ctgggaacaa gatgaaaaag aagaccattt agtgattgcc
    15061 agtgacacga ctgttgttct caatcaagag atcatgggta aaccagaaga tctggctgag
    15121 gctgaattca tgctgaaaaa actaagcggg gaaacacata ctgtatatac agctgttgtt
    15181 ttaaaaacaa aggaacgaga agaacggata ctagcagaag cacatgtcac tttttatcct
    15241 ttgacagacg aagagatcaa acgctacttg gagacggggg attatgcgga taaggcaggg
    15301 gcatacggca tccaaaatgc tgcgagtgta ttcgtcaaag aaatttctgg agattattac
    15361 agtatcgtag gatttccgat tggtgctgtc aatcaggcac tgaaaacatt catttaaaca
    15421 aaaaagcgga ctatctgatt ttcgattgaa aatccatgat agcccgtttt ttttattgaa
    15481 aagattactt atctactctg actaagattt tacttcagct ttaccgactg cttcgcccgg
    15541 gcgaacaagt cctgattggt caagagaaag tcctgcaact ttttcattat tggtcaaagc
    15601 aacaataatg tcagttttct tgccagcttg ttctatttct ggcaaatcca tttcagcaat
    15661 cagggtctct ggtgtgacaa ggtatccttc tttcacgaat acattgaaag gaacaccctt
    15721 tagttcaaca gtatctaatc ccatgtgaac aagtacttcc agcccctcat tagaaagaat
    15781 accaatggca tgttttgttt caaagacact cgtcacttta ccgtttactg gtgcatagac
    15841 tttgccgttt gacggttcga cagcataacc gtcacccatc attttttgtg agaaaactgg
    15901 gtcatttact tcagttattt ccttgatggt ccctgcagcg actgcgaata atgtttcttt
    15961 tgttgcaact gccgtaccag ttgctgaaac aggttcgaaa gactgtggcg ttgctgttgc
    16021 gccatttgct gtttgaagtt gtacatcagc agcattttca ggaacagcag gatcaatctt
    16081 attcaaaatt ccctgacgtc taaagatgat cgtcaagcca aatggaatga tgacagcaat
    16141 gatcattgcg atgatgaaag gtacccatgt ttctgcttgg atagctagta ttcctggcaa
    16201 accgcctact ccgatcgcgt tagctcgaac acccatcaag ttagcaaaca tgccagctaa
    16261 tccagagccg accattgcag cgacgaaagg atagacgtat ttcaaattga ttccgaacat
    16321 agcaggttca gttacgccta agtaacaaga aatcattgct ggaatcgaga tttgttcttc
    16381 ttttttattc ccacgatgta agaagatgat ggctaagact gcagagcctt gggcgatatt
    16441 tgataaagca atcatcggcc ataaattcgt tgatccgaaa tctgcaatca gctgcatatc
    16501 gatggcattg gtcatatggt ggagaccagt aatcactaaa ggagcataca ggaatccgaa
    16561 tacggcacta aacaaccagc tgatagaaga cgtcaatcca gtatttacga tatttgagat
    16621 ccagcttccg actgtccagc cgataggacc taaaataacg tgtgcagcta aaacagttgg
    16681 taacaatgaa aacaatggaa caaaaatcat cgagatcgac tgagggatat atttgcggaa
    16741 gaagatttct agatacgcta acataaaccc tgccagcatg gctggaataa cttgcgcttg
    16801 ataaccaatc atctggactt gagcaaaacc aaaatcccaa aatggaatat ctgctgcggc
    16861 tgtggaagct acactataag catttaataa ttgcggagaa actaacgtaa ttcctaaaac
    16921 gattcctaat atttgtgtag tacccatctt ttttgcaata ctccaagtga tacctactgg
    16981 aagaaaatgg aaaattgctt caccgggtaa ccacaaaaag gcattgacac cattccaaaa
    17041 cttcgagtgt tcaacgattg ttccaccaag actttcaaac tggatacctt caagtacatt
    17101 tctgaatcca aggatcaatc caccgacaat gattgcagga atcagtggtg taaagatttc
    17161 tgctaaaacg gcaatagcac gttgaacggg atgaaggttt tgttttgcag ctgcttttcc
    17221 ttgttctttg gaaacccctt caactccaga tactgcaacg aagtcgttat aaaaagtaga
    17281 aacatcgttt cctatgatga cttggaactg tcctgcattc gtgaacatcc cttttacgct
    17341 aggtatatct tcgatcgctt tttcgtccgc ttttcctgga tcattcagga caaaacgcat
    17401 acgagtcgca caatgactaa ctgcagcaat attttctttt ccgccgatat catgtaacag
    17461 cttctcggca tctgcttgat attttgccat atcaataaac ccctttctct taacttgtct
    17521 atgcaagttg tttcttcatt tactatactt tttttcggag agaattgcaa gcgttttatt
    17581 aaaaagtacg agagaaaatg atatcgtttt aaaaagctta aaataagctt ttcttaaaaa
    17641 taggaacatt gaaatacaaa aagagctgta gtatagtgaa gaaatgatgg aagaacgagt
    17701 gagggaaaaa acatgaataa gttcaatgaa atttttttag atttggaaca gaaaatttta
    17761 gcgaaagaat atcctccgca ttcgctttta cccagtgaga accaattgat caaaatttat
    17821 ggtgtttcac gagaaacaat tagaaaagcg ctgaatcttt tgactacgga aggttatatt
    17881 caaaaaaaac aaggaaaagg ttcgatcgta ctggatcgga atcgcttcga ctttcctatt
    17941 tctggattga ctagctataa agagctacag gagacccaac gtattccaag tgaaacgatc
    18001 atccatacgt tagaagaaac agaagtgaca aaagcattga acgagatcac tggctgggag
    18061 atcggtgcgc ctgtttggca tttgatacgc gaaagaaaaa tcaatggcga agtagttata
    18121 ttagacaccg attatctatt aaaggaaatc gttcctattt taactccagc acaagcacaa
    18181 ggatcgatct atgaatattt tgaaaatgag ctttctttga cgattgctta cgcgcaaaag
    18241 gagatcacag tagaagaagt aacggaaatc atgaaaacag cgatggatct tcacgatgac
    18301 acttatgcag tagtagtcag aagcctcgtt catctggaag atacccgctg tttcgagtat
    18361 tctgagtcgg ttcaccgatt agataaattc cgtttcgttg agtttgcccg acgccgaaaa
    18421 gtgtaaatag ttcaaaagaa agcaaattgt tggcatctgt ccactttggc gctatgatag
    18481 gatcacaaag ggggcatttt taatggataa accaataaga ccagaaaaag aagaactgaa
    18541 agaaaagtta tcacccatgg cttatgcagt cacacaagaa aatgcaacag aacgaccatt
    18601 tagcgggaaa tatgatgatt tttacgaaaa ggggatctat gtagatatcg tcagcggtga
    18661 accgttattc agctctgcag aaaaatatga tgcaggttgt ggctggccat cattcagcaa
    18721 accaataacg aaaagacaag tgcgcgaaaa agccgatttt tctcatggca tgcatcgaat
    18781 agaagttcga agcaaagaag cagattctca tctcggacat gtgtttaccg atggaccagt
    18841 agatcaaggt ggtctacgtt actgcatcaa ttctgcctca ctgaaattca ttccatatga
    18901 aaagatggat gaattaggtt atggggaatt caaatcactt gtagaataat ggaaatacac
    18961 aaagagcggt acaatacggg gatttactcg atcgtccgct ctttttttat tcaagcgatt
    19021 gctatttgaa agaaacgctc cctagactcc tttttttcac aaggtgaaat gtgctacaat
    19081 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    19141 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    19201 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    19261 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    19321 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    19381 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    19441 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    19501 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    19561 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    19621 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    19681 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    19741 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    19801 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    19861 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    19921 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    19981 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    20041 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    20101 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    20161 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    20221 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    20281 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    20341 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    20401 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    20461 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    20521 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    20581 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    20641 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    20701 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    20761 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    20821 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    20881 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    20941 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    21001 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    21061 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    21121 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    21181 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    21241 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    21301 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    21361 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    21421 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    21481 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    21541 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    21601 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    21661 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    21721 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    21781 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    21841 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    21901 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    21961 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    22021 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    22081 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    22141 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    22201 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    22261 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    22321 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    22381 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    22441 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    22501 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    22561 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    22621 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    22681 ttaagttt
//
LOCUS       DAZULG010000041        22685 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000041 DAZULG010000000
VERSION     DAZULG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.041"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..858
                     /locus_tag="ACG9CY_001367"
     CDS             46..858
                     /locus_tag="ACG9CY_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS4937887.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            855..1655
                     /locus_tag="ACG9CY_001368"
     CDS             855..1655
                     /locus_tag="ACG9CY_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS4937888.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1816..3084
                     /gene="rseP"
                     /locus_tag="ACG9CY_001369"
     CDS             1816..3084
                     /gene="rseP"
                     /locus_tag="ACG9CY_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS4937889.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3152..4861
                     /locus_tag="ACG9CY_001370"
     CDS             3152..4861
                     /locus_tag="ACG9CY_001370"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS4937890.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5073..9431
                     /locus_tag="ACG9CY_001371"
     CDS             5073..9431
                     /locus_tag="ACG9CY_001371"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS4937891.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9576..10049
                     /gene="rimP"
                     /locus_tag="ACG9CY_001372"
     CDS             9576..10049
                     /gene="rimP"
                     /locus_tag="ACG9CY_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS4937892.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10072..11247
                     /gene="nusA"
                     /locus_tag="ACG9CY_001373"
     CDS             10072..11247
                     /gene="nusA"
                     /locus_tag="ACG9CY_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS4937893.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11269..11562
                     /locus_tag="ACG9CY_001374"
     CDS             11269..11562
                     /locus_tag="ACG9CY_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS4937894.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11559..11870
                     /locus_tag="ACG9CY_001375"
     CDS             11559..11870
                     /locus_tag="ACG9CY_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS4937895.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11883..14189
                     /gene="infB"
                     /locus_tag="ACG9CY_001376"
     CDS             11883..14189
                     /gene="infB"
                     /locus_tag="ACG9CY_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS4937896.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14216..14563
                     /gene="rbfA"
                     /locus_tag="ACG9CY_001377"
     CDS             14216..14563
                     /gene="rbfA"
                     /locus_tag="ACG9CY_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS4937897.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14720..15176)
                     /locus_tag="ACG9CY_001378"
                     /pseudo
     CDS             complement(14720..15176)
                     /locus_tag="ACG9CY_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15341..17703
                     /locus_tag="ACG9CY_001379"
                     /pseudo
     CDS             15341..17703
                     /locus_tag="ACG9CY_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            18071..18994
                     /gene="truB"
                     /locus_tag="ACG9CY_001380"
     CDS             18071..18994
                     /gene="truB"
                     /locus_tag="ACG9CY_001380"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS4937898.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18998..19939
                     /gene="ribF"
                     /locus_tag="ACG9CY_001381"
     CDS             18998..19939
                     /gene="ribF"
                     /locus_tag="ACG9CY_001381"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS4937899.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20291..21076
                     /locus_tag="ACG9CY_001382"
     CDS             20291..21076
                     /locus_tag="ACG9CY_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4937900.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21089..21940
                     /locus_tag="ACG9CY_001383"
     CDS             21089..21940
                     /locus_tag="ACG9CY_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4937901.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22064..22516)
                     /locus_tag="ACG9CY_001384"
     CDS             complement(22064..22516)
                     /locus_tag="ACG9CY_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4937902.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7698 a         3961 c         4958 g         6068 t
ORIGIN      
        1 aatacttaag atagtgggaa tcttttaagt ataggaggaa aaagaatgtt acgttttttt
       61 ccgcaaaaaa acaaatatat tcaagaaaaa agcgaatttc aatttaatac cgatggacca
      121 attcctcagc acattgccat catcatggac ggcaatggtc gttgggcgca aaaccgtcgt
      181 ttgccacgag ttgctggcca taaagaaggc atggaaacag taaagaaagt cacaaaaaaa
      241 gcatcaaggc ttggagtgaa agtgttgacg ctttatgcct tttcgactga aaactggaag
      301 cgaccaaaag atgaagtaag ttttctcatg caactacctg tcgacttctt tgatacgttc
      361 gtaccagaat tgattaaaga aaatgtcaaa gttcatgtca tgggttatga aaacgtattg
      421 ccagaacata cgcaagatgc tgtaagacgt gcaattgagc aaacaaaaaa caatactgga
      481 atggtattga attttgcttt gaattatgga agcagagcag agatcgtgac tgctgtccaa
      541 gaaattgccg aagaagtagc aaaaggagaa atacacgcag aagaaatcga tgatgaattg
      601 attgctaagc atttaatgac aggtttcttg cctaaagagt tacaagatcc tgagctgatg
      661 atccgtacaa gtggagaaga gcgaattagt aattttctgt tatggcagat cgcatatagt
      721 gagctgtatt ttacaaaagc gctttggcct gattttgatg gtgcgcatct tgaagaagca
      781 atcgcctctt atcaaaatcg agaccgtcga ttcggcggtg taaaaaaaac agaagatgag
      841 aaaggggatc agtcatgaaa caacgtgtgg tcacagcagc cgtggcgcta gtcttgttta
      901 ttccgattat atggatcgga ggcatagcgg ttgagcttac agcagcaatc ttagcagtaa
      961 taggagttta cgagctgttt cggatgaaag gattgacact cttaagtttt gaaggagttt
     1021 tatccgccgt tggtgcagtg ttacttgttt tgccaaaaga aagatggttt ttctttcttc
     1081 cagagaatgt aagtacattt atgttatttt atcttatagt tatgattctt ttaggaacat
     1141 cagttatttc taaaaatacg tatacgattg atgaagcagg ttttcctgtg cttgtcagct
     1201 tgtatgtggg aataggtttc cagaattttg tcaatgcacg aacagaaagc ttggctgttt
     1261 tgatatttgg tctatttatt gtttgggcaa ctgatatcgg agcatatatg gttggtcgac
     1321 gttatggtca gcataagtta agtcctgata tttcgcccaa caaaacgatt gaaggtgcgc
     1381 tcggtggtat tggtagtgct gttgtagtcg catttcttta tttattgcta tacccagcaa
     1441 aagaactatt taatcatggt atgtttgtca tgcttctgat gacgattatt ttttcagcag
     1501 tcggacaatt tggtgatttg gtcgaatctg caatcaaacg tcattatgat gtgaaagact
     1561 cgggaaatat cttaccaggc catggtggaa tcttagaccg tttcgacagt ttgctgtttg
     1621 tatttcctat catgcattta tttggcatta tttagtaagt atacctgcgt ttctttgtga
     1681 atttcataaa agacgcaggt ttttattttt cattatattg atactattgg tgtctttggt
     1741 atactatggt aaaattatag tgttttatcg ttgttttata aaaaacacaa aaaacaagta
     1801 aaggagaaaa aagctatgaa aacgattctg acatttatca tcgtttttgg tatattagtg
     1861 attgttcatg agtttggtca tttcttcttt gcgaaacgat caggaatcct cgttcgcgaa
     1921 tttgctatcg gtatgggtcc gaaaatttat gggcatcaag caaaagacgg gacaacatat
     1981 acgcttcgcc tattacctat tggcggttat gttcgaatgg caggcaatgg ggatgatgaa
     2041 acagaaatgg caccaggaat gcctctttct cttttactaa attctgacgg catcgtagag
     2101 aagataaatt taagtaagaa aatccaattg acaaatgcta tcccgatgga attgagccgt
     2161 tatgatttag aagatgagct gactatcact ggttatgtga atggagacga aacagaagta
     2221 gtaacctacc ctgttgatca cgatgcgacg attattgaaa acgacggaac agaaatcaga
     2281 attgcgccaa aagacgtaca gttccaatcg gctaaattgt ggcagcgtat gctgaccaac
     2341 tttgctggac ccatgaataa ctttattttg gcaatcctgt tattcatcat tctggcattt
     2401 atgcaaggtg gtgttcaagt gacgaacact aatcgcgtag gagagatcat gcctaatgga
     2461 gctgcggcag aagcaggctt aaaagaaaat gatgaggtag tcagtgtgga cggaaaagaa
     2521 atccattcat ggaatgatct gacaacagtc attacgaaaa atccggataa aacattagac
     2581 tttaagatcg aacgagaagg tcaggtgcaa tctgtcgacg tgacaccgaa atctgtagag
     2641 tcaaatggcg aaaaagttgg ccaacttggt ataaaagccc caatgaatac aggttttatg
     2701 gataagatca ttggagggac gcgtcaagct ttcagcggtt cattggaaat tttcaaagcc
     2761 ttaggatcac tatttacagg tttcagttta gataaattag gcggacctgt catgatgtac
     2821 caattgtctt cagaagcagc aaatcaaggt gtaacaactg ttattagttt gatggcgctt
     2881 ctttcaatga atctcggaat cgtcaatctg cttccgattc ctgccttaga tggcgggaaa
     2941 ttagtcttga atatttttga agggattcgc gggaaaccat taagtcaaga aaaagaagga
     3001 atattgacat tagctggctt tggattttta atgttgttga tggtgctagt tacatggaac
     3061 gatattcaac gattcttttt ctagaaaaag ttgacaaata gggtataata acaaacgata
     3121 actattttga ggaaataaag gagtatatag aatgagacaa tcaaaaatgt tgattccaac
     3181 attacgcgaa gttccaaatg atgcagaagt attaagccat caaatcttgc taagagctgg
     3241 atatatccgc caagtatcag cgggtattta ttcatatctg ccattagcaa atcgtgtatt
     3301 ggaaaaactg aaacgcatta tgcgggaaga atttgaaaaa atcggagcgg tagaaatgct
     3361 gatgcctgcc atcttgccag cagaactttg ggaagaatcc gggcgttacg aaacatatgg
     3421 accaaacttg tatcgtttca aagaccgcaa tgatcgaaaa atgatccttg gaccaactca
     3481 cgaagaaact tttactgatt tgatgcgtaa tgaaatcaat tcatataaga aattaccttt
     3541 aaatctttat caaatccaac ctaaatatcg tgacgaaaaa cgacctcgct tcggtttgct
     3601 tcgcggaaga gagtttatca tgaaagatgc gtattctttc catgcaacgg aagaaagttt
     3661 ggacgagaca tataaagatt acgaaaaagc ctacaccgaa gtattcaagc gttgtggctt
     3721 agaatttcgc tcgattatcg gagacggtgg cgcaatgggc gggaaagact caaaagaatt
     3781 catggcgatc tcagaaatcg gggaggatac gatctgttat tcgactgaaa gtgattatgc
     3841 tgcaaacttg gagatggcta caagctacta tgtatcaaaa aaatcgcatg aaacacagct
     3901 tgaattggag aaagtagcta cacctaacgt aaaatcaatt gacgaagtag cagatttctt
     3961 ttctgttgaa cctcaaaaaa tcatcaagtc tgtattgttc attgcggatg aacaacctgt
     4021 ccttgttttg gtacgcggag accatgaagt caatgacgta aaactgaaaa actttttatc
     4081 tgctgacttt ttagaagaag caacagaaga agaagcacaa aaatatctag gtgctgagtt
     4141 tggttctgtc ggaccagtcg gtgtttcaga tgacgtaaaa gtatatgcag accgttatgt
     4201 ccaagattta gcgaatgcca ttactggagc gaacgaaaca ggattccact ataaaaatgt
     4261 gaatccagaa cgcgatttca ctgtactttc ttatgaagat cttcgttttg tacaagaagg
     4321 agatccttca ccagacaaca atggtgttct tgcctttact cgaggaatcg aaatcggaca
     4381 tatcttcaaa atcggcactc gttacagtga atctatggga gcaactgtac tagacgaaaa
     4441 tggacgagaa aaatttgtga ttatgggctg ttatggtatc ggtgtaagtc gtttattatc
     4501 agcaatcgta gaacaacatt cagatgaaca aggaatcaac tggccaaaag gaattgcgcc
     4561 atttgatttg catatcgtcc aaatgaatct aaaagacgaa taccaaacaa acctgacgaa
     4621 tgaactagaa gaagcgatga caaaagctgg ctatgaagta ctggttgatg acagaaatga
     4681 acgcgcagga gtgaaattcg cggattctga tttgattggc tgtccaatca gaatcactgt
     4741 cggtaaaaaa gcagtggatg gaatcgtaga agtgaaaatc aagaaaactg gcgaaatggt
     4801 tgaagtacgt aaagaagaat tggtgaatac tttaccaatc ctcttgaacc aaggcgaata
     4861 ataagcgtta aaacacagaa aataaaaata gctgaatcag ctgtttttga caatctgatg
     4921 attcgctttt aaaattggaa ctgcgatttg aatcgcagtt ccaatttttt aatgtaaaac
     4981 tcagaaaaaa cgagtatact aaaaggtact gaaaaagaag aggataagat gcgatcaatt
     5041 gcattgtttc tcatcagaaa gggcgtatat ctttgtcaga aaaacgagat ttatttgaaa
     5101 aactgttgga acaaatccaa ctagaggaag cagaaaaaaa tcatccactg cttactgctg
     5161 gtgaaatgga tagtgtagtg gtccatcgta aatcgcgttt atgggaattt acgctgcatt
     5221 tttccaaaat tttgcctatt atgctatatc ggtcattgac tcagcatctg actatcgctt
     5281 ttaaagatat tgcacaagtc aaactgaata tcttagcaga tgaccagaca ttcgatgaac
     5341 agctcttgca agattactgg gcccaagctt tggaaaatca gcaatgcgat acaccactag
     5401 cacagcaagt actgaaaacg caggtacctg tgataaaaga tcgtaaagtc atcttgccaa
     5461 tcgacagtac aggagctatt tcttatttga agcaacaata cttgccatta gtagaagagt
     5521 tgttcgtcag ttacggattc ccaaaatttc gtatcgaacc agaagtggat gagcagcaag
     5581 cggaacgagt attgaaactt tttgaagaac gcaaacaaga acaggcagaa gcctttatga
     5641 agcaagcagc tgaaagtcta gttgtccacg aacaaaagaa aaaggaaaga aaagagcaat
     5701 cgccagctct tgaaggaccc atccacatcc agctaggacg aaatattcca gccgatgaac
     5761 caacgacacc aatgatcagt atcgttgaag aagaacgaag agtcacactt gaggggtacg
     5821 tattcgacaa agaagtcaga gaactacgtt caaaacgcaa gatcttgact ttgaaaatca
     5881 cagattatac ttcatcattt atcgtcaaaa aattctcgaa cggcgaaaaa gatgaacaag
     5941 tatttgatgc gatcagtgtc ggtagctggt taaaggtacg aggaagtatt caagaagata
     6001 cgtttgtccg tgatttggtc atgaatgcgc aagatatcat agaagtcaaa catacacctc
     6061 gcaaagatta tgctcctgaa ggggaaaaac gagtagagct tcatgttcat agcaatatga
     6121 gtacgatgga tgcaacaaac agcatctctg atcttgtggc acaagcaggg aaatggggac
     6181 accgagctat cgccattaca gaccacggtg gtgcgcaagc ctttccggaa gcacatagtg
     6241 ctggaaaaaa agcaggtgtg aaaatcttat acggcgtgga agcgaatgtc gtagatgatg
     6301 gtgtcccaat tgcttataat gatgcacatg aagcattgag tgaagccaca tatgtcgtat
     6361 ttgacgtgga aacgacagga ttgtctgcag tatatgacac gatcatcgag ctagccgcag
     6421 tcaaaatgta taaaggaaat gtgatcgaaa gctttgatga atttatcgat ccaggacatc
     6481 ctttgtcacg aacaacggta gatctgactg gtatcacgga tggaatggtt cgaggatcaa
     6541 agtcggaaga agaagtattg cggatgttcc ttgaattttc aaaagataca attttggtag
     6601 cccacaacgc ggcttttgat atggggttct tgaatactag ctacgcaagg tatggtattc
     6661 ctgaagcggc caatccagtc attgatacgt tggaattagc tagatatctg tatcctcaat
     6721 tcaaacgttt cggattaggt gtgttatcta aaaaatttgg tgtcagcttg gaacagcatc
     6781 accgagcaat ctatgatgct gaagcaactg gtcacttagc atggatcttt gtgaaagagg
     6841 caatggacaa tcataacatg ctttatcatg atcagctaaa tgagcatatc ggagaaggag
     6901 attcttacaa aagggctcga cctttccatg taacgatttt agcaaaaaat caggcaggac
     6961 tgaaagattt gttcaaactg atttcaatgt cgaatgtcga atacttcgaa cgcgtcccac
     7021 gaataccacg ctcccaattg aaaaagatgc gtgaaaatct attgatcggc tcagcttgtg
     7081 ataagggaga aatattcgaa gccatgatgc aaaaaggggt agaagaggca agaaaccgag
     7141 caaaattcta cgattatatt gaagttatgc caaaagcggt ctatgcgcct ttgatcgagc
     7201 aagaattggt aaaaaatgaa catgatttgg aagaaattat ccaaaatttg gtagaaatcg
     7261 gaaaaagttt ggataaaatc gttgttgcaa caggtaatgt acattatctg aacgaagaag
     7321 atgccattta ccgaaaaatt ttgatcaatt ctatgggcgg agcgaatccg ttgaatcgtc
     7381 atagtttgcc ggatgtccat ttccgaacaa ccgatgagat gctaactgca tttcactttt
     7441 taggggaaga aacagcgaaa gagatcgtag tagaaaatac gaataaaatc gcagatatct
     7501 gcgaagaagt gattccagtc aaagatgaac tttatacacc gaagattcca ggttcagaag
     7561 atgaaatatc agaattgagt tacacaaaag caaaacaaat gtacggagat ccgttacctg
     7621 agatcattca aaaacgatta aaaaaagagt tgaattctat taatggtaat ggcttctcgg
     7681 ttatttatct gatcgcccaa aaactagtgc ataaaagtaa tgaagatggc tatctagtag
     7741 gttcacgtgg ctctgttggc tctagctttg ttgcgacgat gacaggaatc actgaagtta
     7801 atccgttagc ccctcattat tattgtccag agtgccaata ttccgagttt tttgaagatg
     7861 gtacgtatgg ttctggattt gacatgccag aaaaacaatg tccaaaatgt ggtgcacgct
     7921 taaacaaaga cggtcatgat attccatttg agacattttt aggattccat ggagacaaag
     7981 tgcccgatat tgatttgaac ttctcaggtg attatcaagc agaagcccat aactatacga
     8041 aagtattgtt tggcgaagac tatgtttacc gtgccggaac gattggtaca gtcgccgaca
     8101 aaacagcata tggctatgta aaaggatacg aaagggacaa taatctgcag tttcgaagtg
     8161 cagaagtcga cagattagca aaaggcgcta ctggagtcaa acggacaacc ggccagcatc
     8221 caggggggat catcgttatt cccgattata tggatgtcta tgattttacg ccgatccaat
     8281 atccagcaga cgatcaaaat tcggaatgga aaacgactca ttttgatttt cactcgattc
     8341 acgataatgt attgaaatta gatattctag ggcatgatga cccaactgtt attcggatgc
     8401 ttcaagattt atcagggatc gatccacaaa cgatccctac ggatgacccg gaagtgatgc
     8461 ggatatttgc cggtcctgaa gtacttggag taagtcagga acaaatttat tccaaaacag
     8521 ggacattagg tattccagaa ttcggcactc gttttgttcg agggatgcta gaagaaacgc
     8581 atcctacaac ttttgcagaa ctactgcaaa tttccggact atctcatggt acagacgtat
     8641 ggttaggaaa tgcggaagaa ttgattcgac gaggagatgc gacactggcg gaagtaatcg
     8701 gctgtcgtga tgatattatg gtttatttga tccatgctgg attagatagc gggatggcat
     8761 ttaaaatcat ggaaactgtt cgtaaaggac aatggaacaa gatccctgat gaattgcgag
     8821 aaacctatct atcggcgatg aaagaaaata atgtgcccga ctggtatatc gattcttgtt
     8881 caaaaatcaa atatatgttt ccgaaagccc atgctgcagc ttacgtattg atggctcttc
     8941 gtgtcgctta tttcaaggtt tatttcccta ttttgtatta ctgtgcatac ttttctgtac
     9001 gtgcagacga ctttgatcta gtctccatgt gcaaaggaaa agatgctgtg aaacaagcaa
     9061 tgaaagaaat cacggacaaa ggattagatg cttccgtaaa agaaaagaat caactgacag
     9121 tgctggagtt agccaatgaa atgctggaac gaggattcaa atttggaatg attgatttgt
     9181 ataaatcgga tgccgttaac tttgttattg aaggtgatac gttgatcgca ccatttagag
     9241 ctgtcccaag tttgggaacc aacgtggcaa aacaaatcgt tgaagcaaga aaagacggtc
     9301 catttttatc caaagaagat cttgccaccc gcggtaaagt ttcaaaaaca ttgatcgaat
     9361 atatgaacga caatggcgta ttgaaagatc taccagatga aaatcaatta tcgttattcg
     9421 atatgctata agcatctgtt ttcctgtctg cttgtaattt taagcagagt atggtatagt
     9481 tcttataggt aatgatacta actgtgagtg agcggatttt tcgctcactc tttttaatgg
     9541 aattacgcac ttaatttttt aaggaggcga aacttttgag tagcgtcgtt gaaacagtca
     9601 cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa gtcgaatttg
     9661 taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc ggtatcgata
     9721 tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca gcagatccag
     9781 atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag cggccattga
     9841 aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc ttataccaac
     9901 cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct gaacagctta
     9961 cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga aaaaatattg
    10021 caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa aatgagtaaa
    10081 gaaatgttaa atgcgttaga cgctttagaa gctgaaaaag ggatctcaaa agaaatcgtg
    10141 atcgatgctt tagaagcagc attggtttct gcatacaaac gtcactatgg ccaagcacaa
    10201 aacgtcgaag tggaatttga tcagaaaaaa ggaaagatcc atgtctatgc ggtaaaagaa
    10261 gtaacggaag aagtgatgga ttcacagttg gaagtctcat tgaaagatgc acttttaatc
    10321 aatccagcat atgagatcgg tgatacgatc cgttttgaag taacaccaaa agattttggg
    10381 cggatcgctg cgcaaacggc taaacaagtt attttacagc gtgtacgtga agcagaaaga
    10441 acgattattt ataacgaatt cagtgcttat gaaaaagaca tcatgcaagg aatcgtcgaa
    10501 cgccaggata aacgttatat ttatgtcaat cttggtaaaa ttgaggcagt tctttctaaa
    10561 caagatcaaa tgccaaatga attttatcaa ccacatgatc gaatcaaagt ttatgtttcc
    10621 cgagtagaaa atacgtctaa aggtccgcaa gtatttgtta gccgcagcca tccagaccta
    10681 ttgcgccgct tatttgagca agaagtacca gaagtctatg atggaatcgt tgaaattgtc
    10741 agcatcgccc gtgaagctgg cgatcgttca aaagtggcag ttcgctcaaa tgatccaaat
    10801 atcgatgcag tcggtacatg tgtcgggcca aagggacaac gagttcaagc aatcgtcaat
    10861 gaattaaaag gcgaaaacat ggatatcgtc gaatggaatg aagatccagc tgtattcatt
    10921 gcaaatgcat tgaatcctgc acaagtcatg gatgtgatct tcgatgagac aaatccaaag
    10981 gcctgcacag ttgtcgttcc agactaccaa ttatcgctag caatcggtaa acgcggacaa
    11041 aacgcacgtt tagctgctaa attgactaac cataaaatcg atatcaaatc agaatctgac
    11101 atgacggaat tttatgaaaa tcaagaacaa caaaaagaaa cagaagaact gcatgatgaa
    11161 gcaatcgttc agtcagattt aacagacgat gaatatgaaa caattgcatt caacaatgaa
    11221 acggtcgaag aaaaaccaga agcttaactt tagtaaggag gcaaagagat gaaacaaagg
    11281 aaaattcctt tacgcaaatc cgtggtttca ggtgaaatga aacctaaaaa agagatggtc
    11341 cgaatcaccc gttcaaaaga aggggaagta tcgatcgatc caactggaaa aatgcctgga
    11401 cgaggagcgt atgtctcact tgagccggaa gaagtacaac aagcttggga taaaaaaatt
    11461 ctcgatcgag tactcgaaac aacattaaca gatgagtttt atcaagaact tcttgactat
    11521 gtcactcacc aaaaagcacg taaagagtta tttggaaaat gaatacagtt aatcggcaaa
    11581 aagccatgaa tctcatcggt ctagcgatgc gcgcgggaaa actaatcacg ggtgaagaac
    11641 tgaccattgc tgaaatccgc aaacaaaaag caaaattggt gtttgtggca accgatgcaa
    11701 gcgagaatac aagaaaaaag ataaaagata agagttcata ttatgaagtt ccttgctttg
    11761 agctattttc tgaagcagaa atcacgcaga tgatcggaaa acctcgcaag gtattcggaa
    11821 taatggatgc cggatttgcg aagaagacca aggagctaat tgaaggttag gaaggtgatt
    11881 gcatgggcaa caaaagaatc tatgaattag caaaagaact caaaaaatct agtaaagacg
    11941 tcgtggaaaa agcacaacaa cttggcatcg atgtgaaaaa ccatatgggt gcgattacta
    12001 gcaatgatga gaagaaattg caacaagcgt tcaaaaaccc aagcacgaaa caacaagctg
    12061 cacaacaagc aagcagaaaa ccagccaatc aacagccgaa cgatcaacgt aaaaaagaaa
    12121 caacgaacaa tcaacaaaaa aataaaaata accgcagtta tcaagatcgc ggtcaaggga
    12181 gcggtcaagt gaatcaagga aaaaaaccaa caacaaacca aaataatacg ggaaatacca
    12241 gcagccaaaa tcgtcaagga acgagccaag gcggcaatca aaaccgtact aacaataaca
    12301 acaaaccaaa caataacaat cgcggcaaat ttaataataa taaccgtaat cgttataata
    12361 aaaaaggtaa aaaaggcaaa cagcaacaat caactaaacc agctgtacca ccacgcaaat
    12421 tccgtgaatt gccagatgta ttagagtata ctgaaggtat gaacgtagca gatattgcga
    12481 aaaaaattca tcgtgagcca gcagaaatca ttaaaaaatt atttatgctt ggtgtcatgg
    12541 tcaatcaaaa ccaagcactg gataaagata cgattgaact gttagcgacg gactatggca
    12601 tggaaccaca agaaaaagtc caagttgaca ttgctgatat cgataaattc ttcgaggcag
    12661 aagaagtcga tccagataaa ttagtctcac gtccgccagt cgtaacgatt atgggacacg
    12721 tagaccatgg gaaaacaact ttattggata cattacgcca ttcacgtgta acaagcggcg
    12781 aagccggcgg aatcacacag catatcggtg cctaccagat cgatattgat ggtaaaccaa
    12841 tcactttctt ggatacacca ggacatgcgg cctttacaag tatgcgtgca cgtggagcaa
    12901 gtatcacaga tatcacgatc ttagttgttg ctgcagatga tggcgtcatg ccacaaacag
    12961 ttgaagcaat caaccatgcc aaagctgcag gcgttccaat catcgtcgct gtaaataaaa
    13021 ttgataaacc aggtgcaaac cctcaacatg ttatgcaaga attaagtgag tatgagttaa
    13081 ttcctgaagc atggggtgga gaaacgatct ttgttgagat ctcagcgaaa ttcggccaaa
    13141 acatcgaaga attgctggaa atgatcctgt tagtcgcaga agtagaagat ttaaaagcag
    13201 atccaacaca acgcgcaatc ggaacagtga tcgaagcacg tcttgataaa ggtaaaggtc
    13261 ctgtgtcaac cttacttgtc caagaaggaa cacttcatgt tggtgaccca atcgttgtcg
    13321 gtaatactta cggacgtgtc cgtgtaatga caaacgatct tggccgtcgc gataaagaag
    13381 caggacctgc aacgccagta gaaatcactg gcttgaatga tgtacctcaa gcaggtgacc
    13441 gtttcgtagt cttcgaagac gaaaagacag cacgtgcagc gggtgaagaa cgcgcgaaac
    13501 gtgcattatt agaaaaccgt gctgccacaa gccgtgtgac tttggataac ttatttgaaa
    13561 gcctgaaaga aggcgaattg aaagaagtta acgtaatcat caaagcagat gtacaaggtt
    13621 cagcagaagc actggctgca tcattgaaga agattgatgt ggaaggtgtc cgtgtaaaaa
    13681 tcgtccattc agctgtcgga gcaatcaatg aaagtgatgt aaccttagca gcagccagca
    13741 acgctattat catcggtttc aacgttcgtc ctacaccgca ggcaaaagtg caagctgata
    13801 ctgaagaagt cgatattcgt cttcaccgta tcatctataa agccatcgaa gaaatcgaaa
    13861 ctgcgatgaa agggatgcta gaccctgaat ttgaagaaaa aatcaccggt caaatgatcg
    13921 ttcgtgaaac cttcaaagtt tctaaagttg gtacgattgc aggtgcctat gtaacagaag
    13981 gatatattcg tagagatagc ggtgtccgtg taatccgtga cggaatcgta atctatgaag
    14041 gccaacttgc aagtctgaaa cgattcaaag atgacgtgaa agaagtcaaa atgggttatg
    14101 aatgtggtgc aatgattgaa aaattcaatg acatcaaagt agatgacgta attgaaggat
    14161 ttatcatgga agaagtcaaa aatgactaaa aactgataaa aggaggccaa tcattatggc
    14221 taactaccgt gaccgccgag tcggtcaaga aatcttaaaa gaagtcaatg atattttgcg
    14281 caaaaaggtc cgtgaccctc gtgtggagaa cgtaacgatc acagatgtga aagttacggg
    14341 ggacttacag caagcaacga tctattacag cattttatct gacttagcat cagataagaa
    14401 aacagcacaa gatggattga ataaagcatc aggattgatt cgtcgagagt tagggcacaa
    14461 tctaagtatt tataagacac ctgaattgtt ctttgaactt gatgaatcag ttgtctatgg
    14521 caaccatatc gatgaattgc ttcgcgactt gaacaaagac taatcaaaaa ctcccgcaaa
    14581 tcctgtgaaa tcaggggttg cgggattttt ttgttttcat taagacgtat tacctaagag
    14641 ttccatgact ttagtcttgc ggcgtgtcag ctaaccaaaa aagagaaaaa gaaaagtaaa
    14701 aaattcgact ttcttttttc tagtagtata taaatttttg catatagata aagatcaaga
    14761 taccgataac ggaagctaga ttgtaatcta ttttagtgac agtcaggtat agccaagtaa
    14821 ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg ttgacagtta
    14881 aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga tttggtcacg
    14941 atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac caattcacat
    15001 attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag gaattgctcg
    15061 atccagcccc agttttccag caatgtgtta actggcccag cgtcattcag caaattatgc
    15121 atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag ttttttggat
    15181 caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct gttcatttgg
    15241 ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat tgggagtatc
    15301 tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt ctagtaaaga
    15361 atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg ttgggcaact
    15421 ttatcttaaa gataacccct tactgagacg tacactgaaa cccgaagatg tcaaaaaaca
    15481 tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc atttgaaccg
    15541 agtcatcaat aaatatgatt tgaacatgtt ttatattgaa gggccaggac acggcggaca
    15601 agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc cagaagtaac
    15661 ggaagatgag acaggaatgc aaaaattatt caaacgattt tcatttcctg gaggcattgc
    15721 ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc ttggatactc
    15781 tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg ctgtcgtgat
    15841 tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa atgtctttat
    15901 caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg caaaaatagc
    15961 caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact ttaacggact
    16021 tggctgggaa cctttcttta ttgaaggaaa tgattcagaa aaattaaatc ctgttatggc
    16081 tgaaaaaatg gatcaagcaa tcgagaaaat taaatccatc caaaaagaag ctcgcctgaa
    16141 gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca caccaaaagg
    16201 atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc gagcacacca
    16261 agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac tccgttggct
    16321 gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag aagaaatcag
    16381 agaaatcgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca atggtggaat
    16441 cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat ttgaaaaacc
    16501 aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg agatcattga
    16561 gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa accgattgaa
    16621 tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag aaaatgatga
    16681 atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc aagatgaagg
    16741 gttcttagaa gggtacgttc tgactggacg gcatggtttt ttgcaagtta cgaatcattc
    16801 ttgcgagtgg ttgactctat gttgactcag cacttcaaat ggatgcggaa atcacatgac
    16861 ctttcttggc gaaatgatta tccttcgcta aatctcattg caagctcaac agtcttccag
    16921 caagatcaca atggatatag ccaccaagat ccaggaatat tgacacattt ggcagaaaag
    16981 aaggcggagt tcatccgtga atatctgcct gcggatgcaa atacattgct cgcagtcatg
    17041 gataaagcat tccgctcgtc agaaaaaatc aatttgatca tctcttccaa acatccgcgt
    17101 gctcaatttt actcagcaga agaagcagcc gtcttagtga atgaaggctt gaaaatcatt
    17161 gattgggctt cgacagcaaa agaggaggaa cctgaattgg tcattgctgc tgcaggaacg
    17221 gaatctaact tagaagcatt agcagctgta actttgctgc tggaagaatt cccaaaacta
    17281 aaaattcggt tcatcaatgt cgtagattta ttgaaattac gtcatccaag tcaagatccg
    17341 cgtgggttga gcgatgaaga atttgatcaa tattttacaa aagacaaacc gatcttattt
    17401 gctttccacg gttatgaaac attggtccgc acgattttct ttgatcgtca taaccatcat
    17461 ttaatgattc atggctacaa agaaaatgga gatatcacga caccatttga catgcgtgta
    17521 gtaaatgaac ttgatcgtta tcatttggca aaagacgcag ctttaaaaat caaaggaagt
    17581 caagcagaag attttgcgaa aaaaatggat cagaaattac aagaacatca aaactatatt
    17641 cgtgaaaatg gtattgatct gccagaagta ttggattgga aatggaaaaa tcttgaccaa
    17701 taaaatgata aaatcgatcc cgcaaaatag aattttttac attgattaag aaactgaaaa
    17761 aataaataaa aaaaacggaa aaaagactgg aagaatggcc agtctttttt ttcgtttttg
    17821 ggaagtgaaa accaaactat ttacctgtaa gccacccata aatccaatca aatggtgcaa
    17881 agaaaaaatc ttccattcta ttccctcctt taaaaaagca taacatgatt tctatttacg
    17941 tgagcatgaa ttttgttacg tttttattgc cgaaaaataa aaaccgttca ttatgatgtc
    18001 aaattcgtca gtaattgatg caatgtgtta taataattca gttaaatcaa aaatgaaatg
    18061 gagaaaagac atggacgggt tattaccttt atggaaagaa cgcggaatga caagtcatga
    18121 ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa atcggacatg ggggtacgct
    18181 tgacccagac gtagacggtg tgttgcccat ttgtattgga aaagccacaa aagttatcga
    18241 atatttgaca gattctggga aaatctatgt aggcgagata actctaggtt tcagtacaac
    18301 gacggaagat cgatccggag aacgggtaga aacaaaacca gtagatatgt ctttatctga
    18361 aaaaaaaata gatgaagtga tggcttcatt tattggtgag atcacccaga taccaccaat
    18421 gtactcagcg gtaaaagtca acggtaagcg gttatatgaa tatgcccgca ataatgaaac
    18481 agtggagcgt cctgttagaa aggctcagat ttaccgtttt atccggacaa gtgaattgac
    18541 aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa gtagaatgcg ggaaaggaac
    18601 ctatgtccgc acattagctg tagatacagg caagaaactt ggttaccctg cgcacatgtc
    18661 tgatctgacc cgaacagcaa gtgccggtat gtcccagaag gatgctctga cattagaaga
    18721 agtagctcaa gctgtgaaaa atgggacaat cgatgattgt ttgctgccaa tagaaaaagg
    18781 agtggaacgg ttcaaacgag tagatattac tgatgaagta tggcagaaag taaaaaacgg
    18841 tatgagactt gattatactg tatttgcgct cccagaaatg ccgtcagaag aaattgcttt
    18901 gttttataaa ggaaaagttg tcagtattta tcagccaaac ccgaaagaaa aaaacaaatt
    18961 aaaacctagt aaagtattga gaaatgagga ataacgaatg aatatcatca agatcagaca
    19021 tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt ttagtgctgg gatttttcga
    19081 cggcgtgcat atcggacacc agaaagtcat aaagacaggt atggaaattg cacaaaaaga
    19141 aggattgaaa ttagcattga tgactttcaa tcagcatcca tctattgtct ttaaaaagat
    19201 cgatcctagc agcgtgaaat atttgactag ccttaagcaa aaagaagaaa aaatggccgc
    19261 tttgggtata gattatttat atgaagttga ttttacttct tcttttgctc atttagcacc
    19321 gcaggaattt gttgatcaat acatcgtagg gttacatgca aaatatgctg tttccggttt
    19381 tgattatacg tatggaccaa aagacattgc agatgtcgct cattttcctg aatatgccaa
    19441 aggaagattc aaaatcgtca cagtttctaa agaagaagaa caaggtgaaa aaatcagctc
    19501 tacccgtatt cgtggattac tagacagcgg acaagtaaaa gaagcagcaa aactattagg
    19561 ttgcacatat gaggtagaag gggtagttgt ccatggcgat gcacgaggca gactactagg
    19621 attccctaca gcaaatgtca aagtaaagag cacggttcat ttgcctaaag aaggtgttta
    19681 tgtgtcggaa ataaaaattg gggaaacatg gtatccagcc atgggttcaa ttggacataa
    19741 tgatacattc ggtgaaggac gggagctgac tgtagagttg tatattttgg actttaatca
    19801 agatatctat ggtgaacatg tcgcggtacg ctggaatcaa tttttaagag atcaagtgaa
    19861 attttctagt gtagaggaat tgattgaaca actcaaggag gatgaacaag caacagctga
    19921 atatttttct cagcaataaa acattatgca taaaatctct tgtttatgta taactagcaa
    19981 ttacaataaa atgggtgata aataagccca ggaaaggggc tgtgacaaaa gtcttgtcac
    20041 agcctttttt tccaaataaa tggtttttca gaagtagctt cttcggaaat aagccgaaat
    20101 ttcttgaagc tgaccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
    20161 caattcatga aaacgattgc tagagcgtat catttgcttt agactatata tcttggtgta
    20221 cactcacttt aaggcggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
    20281 agtgggtact atgtacaagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
    20341 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
    20401 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
    20461 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
    20521 tacggcttta gaaaattttg ggacactgga cgtttgggtt aataatgctg ggatggaaaa
    20581 tcagcatcca acccatgaac ttagccttga agattgggaa agagtgttaa aagtcaattt
    20641 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga ataataaaaa
    20701 aggaaatatc atcaatatgt cttctgttca tgaacagatc ccatggccaa cgtttgctca
    20761 ttacgctgct tcaaaaggcg gggtaaaatt atttaccgaa acggtagcaa tggagtatgc
    20821 accgaaggga atcagaatca atagtatagg ccctggtgct atccgtacac ctattaatgc
    20881 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
    20941 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
    21001 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
    21061 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
    21121 tggctggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
    21181 attaggaact ggtattggag cggtgcttgt cggcttactc gttcaattat tcgcttctcc
    21241 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tttgggttat
    21301 tggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
    21361 ttcgactgca ttacagttag tcggaacctc gttgattggt gtcttcgctt ttggagaatg
    21421 gtctggaagt atggggaaaa tcattggagc agcagcaatc attcttcttg tatttggttc
    21481 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaattag
    21541 tctattagca tcaacaagta ttggttattg ggtatacagt gcattgccta aactagttga
    21601 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
    21661 ttatgtgatt ttccgtaaac caaccgcatt ttctgaagac aagagttgga aagcaacgat
    21721 cgttggagcc atctttagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
    21781 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
    21841 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
    21901 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaattt
    21961 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
    22021 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagggaaa
    22081 tatctgtctg gatcaataat ttttcattga tccattgact aaagtttacg acataattga
    22141 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
    22201 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggaattgat
    22261 tgattgctgt aaaatcacga tattgaaggt tgaaacgcat cgtgattggt gcatttagca
    22321 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
    22381 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
    22441 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
    22501 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
    22561 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
    22621 cagaaaattc taacttttat ttctctaaag aaattcgttc tctcctaaag tgatattata
    22681 tttaa
//
LOCUS       DAZULG010000042        22224 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000042 DAZULG010000000
VERSION     DAZULG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.042"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..655)
                     /locus_tag="ACG9CY_001385"
     CDS             complement(137..655)
                     /locus_tag="ACG9CY_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4937903.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(657..1274)
                     /locus_tag="ACG9CY_001386"
     CDS             complement(657..1274)
                     /locus_tag="ACG9CY_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4937904.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1271..2059)
                     /locus_tag="ACG9CY_001387"
     CDS             complement(1271..2059)
                     /locus_tag="ACG9CY_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4937905.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            complement(2121..3032)
                     /locus_tag="ACG9CY_001388"
     CDS             complement(2121..3032)
                     /locus_tag="ACG9CY_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4937906.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3073..4407)
                     /locus_tag="ACG9CY_001389"
     CDS             complement(3073..4407)
                     /locus_tag="ACG9CY_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4937907.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4615..5445
                     /locus_tag="ACG9CY_001390"
     CDS             4615..5445
                     /locus_tag="ACG9CY_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFS4937908.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5689..5938)
                     /locus_tag="ACG9CY_001391"
                     /pseudo
     CDS             complement(5689..5938)
                     /locus_tag="ACG9CY_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6129..6323)
                     /locus_tag="ACG9CY_001392"
     CDS             complement(6129..6323)
                     /locus_tag="ACG9CY_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937909.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6828..7013)
                     /locus_tag="ACG9CY_001393"
     CDS             complement(6828..7013)
                     /locus_tag="ACG9CY_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937910.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7863..8204)
                     /locus_tag="ACG9CY_001394"
     CDS             complement(7863..8204)
                     /locus_tag="ACG9CY_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4937911.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8204..8434)
                     /locus_tag="ACG9CY_001395"
     CDS             complement(8204..8434)
                     /locus_tag="ACG9CY_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4937912.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8721..9059
                     /locus_tag="ACG9CY_001396"
     CDS             8721..9059
                     /locus_tag="ACG9CY_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937913.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9264..9826)
                     /locus_tag="ACG9CY_001397"
                     /pseudo
     CDS             complement(9264..9826)
                     /locus_tag="ACG9CY_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(10558..11139)
                     /locus_tag="ACG9CY_001398"
     CDS             complement(10558..11139)
                     /locus_tag="ACG9CY_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4937914.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11322..11948)
                     /locus_tag="ACG9CY_001399"
     CDS             complement(11322..11948)
                     /locus_tag="ACG9CY_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4937915.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11970..13301)
                     /locus_tag="ACG9CY_001400"
     CDS             complement(11970..13301)
                     /locus_tag="ACG9CY_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS4937916.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13456..13857)
                     /locus_tag="ACG9CY_001401"
     CDS             complement(13456..13857)
                     /locus_tag="ACG9CY_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4937917.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13943..14149)
                     /locus_tag="ACG9CY_001402"
     CDS             complement(13943..14149)
                     /locus_tag="ACG9CY_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937918.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14244..15746)
                     /locus_tag="ACG9CY_001403"
     CDS             complement(14244..15746)
                     /locus_tag="ACG9CY_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS4937919.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15764..16219)
                     /gene="argR"
                     /locus_tag="ACG9CY_001404"
     CDS             complement(15764..16219)
                     /gene="argR"
                     /locus_tag="ACG9CY_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4937920.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16317..16934)
                     /locus_tag="ACG9CY_001405"
     CDS             complement(16317..16934)
                     /locus_tag="ACG9CY_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4937921.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17155..18528
                     /locus_tag="ACG9CY_001406"
     CDS             17155..18528
                     /locus_tag="ACG9CY_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4937922.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18603..19715
                     /locus_tag="ACG9CY_001407"
     CDS             18603..19715
                     /locus_tag="ACG9CY_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4937923.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19787..21424)
                     /locus_tag="ACG9CY_001408"
     CDS             complement(19787..21424)
                     /locus_tag="ACG9CY_001408"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS4937924.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21592..22200)
                     /locus_tag="ACG9CY_001409"
     CDS             complement(21592..22200)
                     /locus_tag="ACG9CY_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4937925.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6484 a         4531 c         3872 g         7337 t
ORIGIN      
        1 taaaatttta taacgttctc atttaacaaa acttaagtta ttaagacaaa tgaataaaca
       61 actacgcttt cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc actcatgctt
      121 tattcgtaca attaattcag ataacggcag cgcataggat gccaataaca cttcctaaaa
      181 tagctcctcc taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc gaataagcga
      241 tcaaacaagc aaataaaaag gctaatattc ctgcaactgg acttaattcg aacaacgcag
      301 ctactgataa aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac
      361 ttgtgtaaag gacaagccga cgattttttt cataaggtct tttccgtctt gtcgtttttt
      421 tgataaaaag caaaccaaca aaaaaataga tcaaactgcc gataagaatg gctagaccat
      481 attctctagt atcaggtatc aagactaaaa cagcagcaag gagcaaccaa ataaaaccac
      541 cattagagat attactgata aatttcaaaa aattgatttt tcgactatcc gttttccgtt
      601 gcttggcgaa cttgttgata atactatcat cgattttagc aatcaaacgc aacatgttat
      661 tcctcctttt taagaacctt ttcttgcgta cgatggataa aaagcaggac ttgaaaataa
      721 aacaagaaac tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca
      781 agaaggatca acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag
      841 aaaaatgcag taatccaaga agcttctttt tgttccagtt gaagcaagat tttgtaacta
      901 aagagaagcg cgcttaatag aagcaaaaac aacgtagtat acatcaatgt atagccaatt
      961 cccccacccc aatagcggaa aatctgataa tcgatcaata gaagaagaaa ataaacactt
     1021 gctaaactat cttgctttcc ataaagagag aagttctcac gaaacttttt ccagaatagt
     1081 gaaccgagct ttttctcttc attttttttg taatgataaa tcatatacag aactgttcga
     1141 cttgcagttc ctaatgtgaa aacaggcaag acgcacagga accatagtag ctgtaaatgg
     1201 ataagacgtg gaatccattc aatgatttct aagaaataat cattggcttt tttaccaatc
     1261 gatcgattca tcatcctttt gaacctcctg tcaaatccat cccttcaatg aaataacgtt
     1321 gtgcaaaaaa gaacagcaaa atcgtcggga tcaagacaag tgttgctcct gccatcagat
     1381 aattccattg tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt aacgtgtatt
     1441 tttcttgatc ttggatatat aacagaggac ctaagaaatc attccaagct gcgttgaagg
     1501 aattgatcgc aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa tgactccaaa
     1561 tatacaaatg attcgctccg tcgatttcag cagcttcgat caattcatta ttgatcgata
     1621 agtaaaattg acgcatcaga aatatattga acgcattgcc gaagaacgat ggaacgatca
     1681 ggggcaaata agtacctacc cagccgatct tggagaataa tacatattga gggatcatcg
     1741 ttacaaaccc cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc tttttcccag
     1801 gaaaatcaag cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca ttaccgatca
     1861 ctactagcac agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga aaggcggcaa
     1921 tcgtatccag atagttttcc catacgattt tttctgggaa aaaggtcgga ggataacgaa
     1981 cgatttcttc cattggtttc aatgaagtaa acaccatcca aagcaatgga atgatcaaaa
     2041 tgattcctat tactgtcatg aggacataac ttagtatttt tttgtttgcc gattcacact
     2101 ggtctttttg aaataatcca tcatttgccg tcacctccag aataatagac ccacttaggt
     2161 gcaagcttca aattgacaaa cgtcagaacc aacacgatca gaaacaggat cattgacata
     2221 gccatcgcat aacccatatc aaaagctacg aacgccttgt tccacatatg cagattataa
     2281 aaaagcaaag aatttgcagg tccgcctgtt ccatttttcg tcatgacata agcttcttga
     2341 aaaatttgga aagaaccgat cgtagaggtg atcacatcgt aaaaaataat aggagtgatt
     2401 aatggcaaag taatactttt gaactttccc caaacgcctg ctccatcgat ttctgctgat
     2461 tcatataaag atctaggaac attttgtaat gtagctaagt aaagtagcat tgctcctcca
     2521 gaactccata ttttcatcaa aatcaacgca ggtttcgtcc attgtggatc aaacagccaa
     2581 gctggtccag ggatatggaa ccagcccaga aatgtgttga tcaatcctgc agatggagaa
     2641 agcagctgca tccataagat gtagactgct actcctgata agactgccgg taaatagaaa
     2701 gctgtacgga atacactgat tcctttgatt ttttgattga gtactgtggc taaaaacaca
     2761 ctgaaaatcg tcgtcagcgg tacattgaag atgacgaaat aaacggtgtt gtataacgat
     2821 ttccaaaata aatcatcgtg aacgaacatt cttttgaaat ttgctagacc aatgaaattc
     2881 atcttggatg tcaaattata atcggtgaaa ctaccaataa ctgaaaaaag cataggccct
     2941 agcataaata ccaagaaccc gataataaaa ggcgagataa ataaataggc ccatcttgct
     3001 tcttttttct ttcggataga taaacgcttc aaattttctt gcctcctctg tacctagaca
     3061 gtcaaaaatg gattattgat tttgcttgac taagttttct actgcttttt gtgcatctgc
     3121 caatccttct tttggtgatt ttgttcctaa aagaatctgg tcaatctgtt cattcactaa
     3181 tgtcgtgtaa tccggagcat ctaaaggaac tggtgtgatc accgtatttt taaagttttc
     3241 gtccgccatt tgatagatca tttttccttt atccgttagg ttgtcattat tcactaaatt
     3301 ctcattggct gtcttgctcg ccatgatatc aaaacttttt tcgccgaatt tttcttgtac
     3361 ttctggagaa gaaagatatt tgatgaattc ataagaggcc tcaggatctt ttgctccgta
     3421 tggtacttcc aagacgaatc cgccacccca cgaccaatgg cctgaacctt cttctttttc
     3481 tgggagctgc atcacgccaa aatcaaaatc atccggtgca ttttccatca aactagagta
     3541 ataattgata ttttgggcac gcatggctac taatccggaa ataaatggat cagatacacc
     3601 agacccaaat tcggcttcta atttattgat cgtatttttc ccataataat cttgccagtc
     3661 ttggatccac tccaaggctt ccactttatt tttagtatca atcttgactt tttcatcttt
     3721 gtcaaaccaa ctagtcccgt tatctgcgtt tagcgcccat acatcagccc ctaaattcca
     3781 taatggataa aaaccgatgc gtgtaaattc ttttccttct tttttatcta gtttacgtgc
     3841 tgtttcttct aattcatccc aagttgtcgg tagatcttgt tcactgattc cagcttcttt
     3901 aaataacttt ttattataaa aaatgacttg tgtatctgta ttgaatggga tcgcataagg
     3961 atcaccttca tattctactg tatcccaaag ctgcggataa aaatccttag aagtgtcttt
     4021 ttctacatat ttggagagat tagttgcttg ctgtgcttct gctctttggg caacggaatt
     4081 tatgtcctgt acaataacat cgggtgcatt tccagctgtt attgcagata aagatttagt
     4141 ccagatgtca ccccatggct gatacttata atttacatgg atctcgtttt gtgattgatt
     4201 gaaatcctca atgatttctt cgatagcttc ttttcgtgca ccggatcccc aaaaggacca
     4261 gaaatcaata ttttttcttc catcacttgt tttttcagaa gaatttgctg cacttgaact
     4321 ggcatcgctt gcattacttg atccgcatcc tcctaataaa aacaatgctg aacttgccag
     4381 caaccctgta aacaataatt ttttcatatt ttacctccaa attttaatgt aaccgatgcc
     4441 ggttttaata taacgatatc attttgttct gaaaataaca atttataagg tatgattcta
     4501 aaaaaattat taatgatacc aatactttat ataattcgcg agcgctttct ttgaattttt
     4561 taatctttcg tagcagaatg agaatgtatt aaaaatagat taggaggaat tttgatgttt
     4621 agacaccaga aagaattgca gtttgaagca aaaccagatc gtccgaatcc attaatcgcg
     4681 aaatatctgc aggagctgat cggcggccaa tatggcgaga tgagtgtagc tatgcagtac
     4741 ctgttccaag gctggagctg ccgaggagaa gaaaaatata aagatatgct gatggacatt
     4801 gcaacagaag aaatcggcca cgtggaaatg ttggcgacaa tggttgctcg tttattagag
     4861 ggtgcgcctg ttgaagatca agaagcagcg atgcaaaaag atccaacaat cggtgccatt
     4921 atctctggaa tgaatccaca gcacgcgatc gtagctggct taggacctcg tcctgctgac
     4981 agcgtgggca atccatggag cggtgcctat atcattgcga gtggcaactt gttagcagac
     5041 tttagagcta atttaaatgc cgaatcacaa ggaagacttc aagtagcacg tatttacgaa
     5101 atgatcgatg atcgtggcgt aaaagatatg ctgtctgttc tccttgcaag agatagctat
     5161 catcaaaatc tatgggaatg tgcagtgaaa gaattagaag aaaaagaagg ttcaatctta
     5221 gtcccaagta ctttcccgcg tgacaaagaa cgaaaagaca ttgcttatga cttctataac
     5281 ttctcagagg gagaccaaag tagtgaaggt tcttgggcta gtggcaaagc actggatggc
     5341 gaaggcgaat atcaatggtt gaaagaacct aagatcgaag gcgctgcacc aaaattagaa
     5401 ccagtaacac caaaaatgta tgggacacct ccattcaaga aataaatatc tacccgtaga
     5461 aacacccgtt ttttgttcca cacgctttgt gagcaaaact gactaaagtc attaatcata
     5521 accacccttc aaaagggtgg ttttctctag agctataagc tcaaatcacc ggccagcgcc
     5581 taaagacgct ggctttcata ttttatgttc aagccatatt gcctttgcca gttttgccca
     5641 cctcttaaaa ggtttatgta ctactcctta gcccttaaaa ggatcgtctt actctcgtac
     5701 actcagctta tctaacgcac tatcatgttt ttcttgttct tgaatgtact ttttgatggt
     5761 cgcttcattt tgtcctactt ttgtcacata gtatccttcc acccaaaagt gtcgataccc
     5821 aaatttatat tttagatttg cacaatctct tgatgatctc ctgaatattg gctcgatatt
     5881 gattataaat tatttttctt ctatactttg gtgtgaatac aatgtggtat ttacacatct
     5941 actttgtacg ggctaaacta tttcccttta ttgctccacc cgcatagcaa gtggttttgt
     6001 atttcaatcg ctcggcgcgc tcaattaggt ctaaagacaa taaatctaaa aacccgctgc
     6061 ttcactgaca gaaacaacgg gttttcagaa aaaaatgaaa tgaaaaagat ctcttacggc
     6121 aatctatttt atttttttga ttttttataa atggaaaatg cttcaagaag tccaagaaat
     6181 aaagccgtat aaaaacaaga acctatgaaa tctttattta ttagatattc aacagaaatc
     6241 ccgataacac tagccaacaa tatatttatc agaagtgtaa aataaggatg ctttttatag
     6301 aatagaacta gtgttgtttt catagactct cctttcttca tatttccaat taatcacaaa
     6361 ttcacggcat tactagaaaa attaaagggg cgcagaagtg gaaaaatact cttcgacttt
     6421 ccctacaaac caggccatct tgtgtttaat aactatacca aatctaaggt acctctaaat
     6481 ctaaattcca tttgtggcgg tgacctccaa tttttaactc ttctatctcc catccagtat
     6541 acataacctt gatacttcga ccacttatat atcgaacaca caaataccta tccattgcag
     6601 gagtactacc taacttagtt gctacttgca tctaaataac agctgaaaag ataggactta
     6661 ctcttagtaa agtatctgaa ctattaattt ttaaactatc atttttatcc tcaactacat
     6721 gcacatctgt tgcaacttca ctttctgtct catccagata aatgattaag gaatcgggaa
     6781 ttgcaatatc ttcagctgac gctggaatag ttgagctaaa ttagtcatca tatctttatt
     6841 ataaaataac caacagcaaa aaatacaatg aaaaacaaac ttggaactaa tgtatcatta
     6901 ataaatgttg aaaaattcag caactcatta ctgtatgatt ttagcagccc aaccaacata
     6961 taccctataa aagctaataa gatggttagg aatcctttag aatttttact cattcataga
     7021 cacttcctta aataactttt attatagaag gtattgtaaa taataatatt gacaaaaagt
     7081 gactcaaaat aaaaaaaacc ttacattata ctggaatgaa ctgtattgat agtgtaccta
     7141 aaagctctac tgacataaga tcgtgtaatc ggtaaagtaa ttttattaat tatacgggat
     7201 gtaatcaatt agagaataaa ctaatcataa aaggtacttt tacattcata attgtcgctt
     7261 tgagattcta ctttggttca ctatctgacc caattgcaca ttttcgatca ttacaagtat
     7321 tggtgtcgtt agaacaacta aataaaaact agcggtagat ttttcagaat ctatgctaat
     7381 ttctgtatgg atgttccagt gatggcaaat ttctcttttc attgtttttc ttgtcgcatg
     7441 atatgccttt ttgtgttatt cagttaaact atagaaattc tttcctagac agatcttgat
     7501 cccaaatttt acgtttttga gcatcgatat agtttaaaat gacattactc atatctaaag
     7561 agtttagatt ttttaggact aaaactgtca gttgttcttt ttcttctttc tgttaaagta
     7621 ttgtcgcgac ttttcgtata cgttaagata tgtcgtgaaa aaacaagttt tgtgacacaa
     7681 aaaaacgaat gacttgagga aatcacctca atcattcgtt taaaatgcta tatattcttt
     7741 agtttaagat acggaaatta atgccatcga ccacactagt agaatgaacg acatttactt
     7801 ggcctttttt taatttcgtt atgtttagca tataaaaata ttatagagtt tacttacaat
     7861 gcttaaaaca tatactgtat aatctgatca attttagcca tgtcttgcaa tggtaagttt
     7921 tctatttttt tgagttttcg ttccttaaaa tcaagagatt ttaattgtgt aataattact
     7981 tgtcctttta tatctagctc ttcaggtaat gagtaacgtg tcggtaaatt ctttatagtg
     8041 gaagtaattg gacatatgac cgaaaacatt gttttacggt taaattcata acgagagacg
     8101 actagtcctg gtcgtctttt ttgaatttct tttcctacgg agggatcaaa atcaatccat
     8161 acaatatcac cttttttagg aatataaatt ctttcttctg acactataaa atttctctcc
     8221 cttcaggagt taaatcttcc cattcgtctt tctcataata ctcggcctct tccccaccac
     8281 taaatggatc ttcaattttt ggaactaacg taattgttcc atcatcagaa tacacaacga
     8341 tatattcttg gttttctgaa ggtttctttc cgttatcaga aggtaaagtt accactacag
     8401 aacttccttg taatctagat tttgtaacaa gcattatcat cactccctta ataaaagtat
     8461 actatataac actaagtaac gcaaaattcc cctttgtaaa ctatctagaa aattgttttg
     8521 tttaaagtat atcttattgg atataaatag aaataaattg tagccagttc attttttctt
     8581 taactaaaaa aataatccaa gaaacattga acctatggat ccaaaagcta cccaaatact
     8641 atagctaacc ccccgctacc ttcaattttg cagataaaac gctttgggag aaaatttcaa
     8701 agacaacagg ccatcctatc atgcccgatt ataaacaaaa agctggctat tccttttgct
     8761 ctgacaaatt tcaattggga aagttcacga aactagcagg tgaagaagtt tcaactgttc
     8821 ggatcaaaga atgcccgctt caaatagaaa cattggttca acgaatttca actagacaag
     8881 actttgctat tgtagaacgt gagatcgtag ctatttttgt agaagaaggg attttacatg
     8941 atccaactca tatcaatgtg gataaatggc aacctcttat ctataaattc agagaatata
     9001 caactgccaa ccagtcgtta actttgaatt ttagattcca agaattcaaa gagtcataat
     9061 gataaacatc gttaggtata aataaaaaag actcgcagca cgagtaaaaa acaaatagaa
     9121 gctatacatt agaaaggtcg actgtgaccc tatagatgca gtatatgcag cttatgagca
     9181 tttcttctgt tctctttgtt tttgggatct gtgctgaagt ctttttcgtt ttttgccgct
     9241 ctttctttga aaaatcgatt atgttatacc ttctccatta ctaattcagg catctgctga
     9301 gtaatgattt ttcgcgcaaa agaaatatct gttacccaag gttcgatttg ttcttcagtc
     9361 aagacaagcg gcattcggtc atggattggt gcgactgtgc ggttaggttc agtagtcatt
     9421 atgattgatt ctgccatttt ttcatcctga tggctacgat aaaaaccgcc aatatacact
     9481 attttttcct tatttgaaaa cataaatttg tctttttctt cattccactc attgggaaaa
     9541 cacaacgatt ttgcaaaaat ggttgatgaa aggtcttttt ttcttctact gtctctgcac
     9601 gggcattaat catcaattgt ccttttttaa atccggtaaa tccccacttc atgatttccg
     9661 gagtgaggat gttttcttcc tcttttctta acgtcacaac aagatttgac ggaaaaactt
     9721 ctccagctgc taattctgct gcctgcttcg gttgtttcgt ctgggcttct cgataataat
     9781 tctgaaggag ctctgactct agatcaaaaa aataacgacc acacatccta atccctccca
     9841 atcttttttt ctatcataca ctgcaacaag aagttcgcac caacaaaacg ctttcaattt
     9901 ttgtagaact aaaaaagttg tgacaagtga caaactctcc agtgaatggt attctcctgc
     9961 caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgctt tttgaaattg
    10021 ttccttgtga ctcggagtga aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa
    10081 gccagtttct cggtattaag ccaaatttat gcaaaaatgt agaaagctat tttcacaaaa
    10141 tttttcttaa tactcgaaac taagcgactt tttcacaacc tcttccttaa acggtgttca
    10201 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagcag ctatttttat
    10261 ttggctgttc ttattacttg atgcagggca gctttttcac aacctctcca ctaaacggtg
    10321 ttcaatcagc taaccttcgg tattagctca aaaaacggca aaatatgagg aactattttg
    10381 ccgtttgttt ctctaatact caggtcaaag cgctgatttc acaacctcta atacacggtg
    10441 ttccagaagt agcttcttcg aaaaataagc cgaaatttca caaaaatttg agaagcaatt
    10501 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct ctaattatta
    10561 aagaaccttt tgcgaagtta acaatctttc tttgatcaat tgaaaattct gcctaagtag
    10621 tgatagatcc tgtgtgactt tcaacaataa gatacttccc tggatttcag caatcaacgc
    10681 tttggcttga cggtctgctt cttcatcgga cagtttttct tcagaaagtg ccaggatatt
    10741 ttgtttcgta tacttgaccc attgattgac taattctagt agcaacagac ggtattcttc
    10801 atccactgta ctcaactcaa taatcaaatt acctacagga cagccatgat aagtgatctg
    10861 tgtctcatga tcagcgaaaa tccagtccaa catttcttct aatgcttctt ctggctgttt
    10921 tccagcattt aagatgtctt cgaacaattc tgtctgccat acgtccatat gcgcatggat
    10981 caccgctaga caaacttctt tttttgaaga aaagtaataa taaaattgac cttttcctgt
    11041 atcagaagct gctagaatat ctcggattga cgtttcatga tagccttttt tataaataag
    11101 atctttggtc gcttccaaga tcttttcctt tgttttcaat gatattctcc tttttcattt
    11161 ctcatagtcc tatattatac agtaaattca tcattttttc ttcttaatcg tttaaaaaag
    11221 actgtgccaa attatctgac acagccctca ctataaaact gttctcatat agctagatgt
    11281 tcattgattc aagaactgcg aggacaaaag caattcctat cttatgatag ttgctctaat
    11341 acttcctctg ttgtcatcac ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc
    11401 attgcttctt ggccaggagc accaattgca tctgtcgcta ccacaacatt gaatccactc
    11461 tctaccaatt cacgcatatg ggaatcgcta cacaagtttc cgttcattcc accaagaatt
    11521 actgtgtcga ttccattttt tctcaattgg agcgccaaat cattgctttc tggtccaaag
    11581 attttatgag gagagctgat gatcgtattt tcatccaaat aaggtttcaa ttcttcgata
    11641 aaatcggctc ctgaaccttc ttccactgat tcatattgac tttttctagc aaacatctgc
    11701 tgctcaagca tcatatgttc tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa
    11761 aaataatgag gagaaataaa gagtttgtat cctttttctt ttgcttgttt taccatttca
    11821 atcatatttt cgatcgtatt gtatttttct aagatatctt ttgtcaaacc aaagccaccg
    11881 cctgctggtt tcaagaactc gacttgcggg tcagtgagaa ctactgctgt atttttatta
    11941 ttgaacatgg tggattctcc attcgttatt tattttaata catcatttag agcttcactc
    12001 attgtcggat gagaatagat ttgatctctc aataacgtat aaggaagttt tccattcata
    12061 gctagcgaaa tcaagttgat gacttcatga gaatcttccg catagatact tgcccctaga
    12121 atcaagtcag tttcagggtc gactaaaact ttgaatacac ctttaggatc ttctaatact
    12181 tgtgctttag ggacaccgct tgtcatatac ttgaataatt tgtaattgac gccttgtgct
    12241 ttggcttgtt tttctgttaa acctacattg gataaagtcg gagtgataaa tacactgtaa
    12301 ggcacagctt tccggttatt cgttgttcgt ttattctcgc ccttcaactg atccataatg
    12361 attcgataat catctagaga gatataagtg aactgcaatc cgcctttgac gtctccaatc
    12421 gcccaaatgt tgtctgcatc tgtacgaagg tattcatcca cagcgattgc tccgcgatct
    12481 gttagtttaa catctgtatt ttctaatcca agtgatgctg tacttggttt tcttccagtt
    12541 gcagccaaaa cttttgacgc ttggattgtt acttcttggc catcttctgt aaaggtgacg
    12601 gcagctgttg attcttggtc tgctacttga tctacagata cacctagatg gaattcgatt
    12661 cccgcatttg tcatgtcttc taaaatcatt tgcgcgatat cttcatcttc acgtggaaga
    12721 aattcagaat gggcatcaag aacgacaact ttagatccgt attcattgaa cattgatgca
    12781 aattccaagc caatatatcc agccccgatg atcactaatt tttccggtaa ttgttcctga
    12841 tccatcgctt gcgtagaatc aatcaagtag cgacttgtat tcaatccttt gattggtaaa
    12901 atgattggtt cagaaccagt attgatgaaa atccgttcgc cttcaacaat cactttttca
    12961 ccattattcg tcacttcgat ttgatggtta gaaagaaaac gtgctgtacc gtccatcact
    13021 gtcccagttg cctcatcgct gatcatatga taatttttgg cgcgaagcat gcccgtcaat
    13081 ttttctttcc gagcaacagc ctctgtaaag tcgattcctc tttctccatt gaaaatcaat
    13141 gatttagaag gaatacagcc gatgttgata catgttccgc catacatacg tgttgacttt
    13201 tcaacgacta aaaccgattc accttttgaa gctaacgcct tcgctaaagt cttaccgccc
    13261 tttccaaaac cgatcacaat attttgatat ttttccatca tttttccctc gcttcttttt
    13321 tacctaccga ttggtacaaa aatatgataa ccgataattg tctattctcc aaatcatctg
    13381 ctttatttgt caaaaaaaac cagacaaaaa gcggtccttt cacgttttta gtctggttcg
    13441 atttccttta ttcttctaaa caatcgttac ttctacatcg atatttcctc tagttgcttt
    13501 ggagtaaggg cagaattcat gcgcatcttc tgcaattttt tgggcttctt cttcgctgat
    13561 gccatcgata tgcacttcta atactacacc gatctgatag ccgccatcat ctaaattata
    13621 aagagagaca cgtgctttta ctgtggactc tgctgtcatt cctgcttctt tgatcatatg
    13681 ttgtaccgcc ccattgaaac aagaagcata acctgctgca aataattgtt ctggatttgt
    13741 tccttctgca tggattcctg gaggagtgat tttcatatgc atcgagccat ttggtgcctc
    13801 gacttctcca ttgcgcccgc catggttgat cattgctgtt tcgtatgctt ttttcattgt
    13861 actgctcctt tcgattgacc atttgctact tggctataat tatagtgtat gacaagtaag
    13921 gcgctttcta caaaaattct gcttattcga ataccagaat gccgtcttct actatttttc
    13981 caaaaaacaa aaaaatacta ctcgtgaaca acagcgtcat atttgttcca aaataaatag
    14041 caatcttttc tacataatct tttatatgat tgattttaag atacatatat accagaaagt
    14101 tgaccccata aaccaatact gttaaaaaaa taaccatatt cgttaccatt ttttcctcct
    14161 tttgctggtc tccctaccag cactcgattt aaccatacca ctttttttgc cgtccaagca
    14221 actagccttt caaaaagact ggtttattct gctgttaatg attcaagatc agaaaaatgt
    14281 ccgaataatt ttgctaattt tactaacgcc aacttgttct ttccgaattc atcattttct
    14341 atctgtaata ctagtaacgg ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg
    14401 tatggctctt ctagatcata tcgaatacct tcttcatttt ccgggtcgaa gttcatccct
    14461 ttttcttctt ccaacagtcg cttcatttct tctaatatct tgtttccttg ttcgcgtggt
    14521 tcatcgctga acaattttaa tcggatttct tgtacttcta cgggctgttt caactctttg
    14581 atttgttcat ctaatgtgat accttttctt tttaattctg gcaaaagcat caggatcttt
    14641 gcgatgacat atgcaccatc atccagaaaa tagttttcct tgaatgcagc gtgtccgctt
    14701 gtttcgatgg ctagctgtgt atctttccca agacggttaa gttctaatgc tttattgatt
    14761 acatttcgat agccggaaat ataacgaact tggatgcctc ctttttcttc aatgaatctt
    14821 tgtagatgag aagaagtagg ggaattcgtc acgattcttg ttcctggatg ctcttttaat
    14881 acgattgtac ttaatactgc gatcaaatta tttcgattga tcactgttcc agaagcactg
    14941 acaactgcag aacgatccac atctgtatcg aaaatgatcc caagatctgc ttcatttgct
    15001 aaaacagcct gcttgatact ttccatcgct tctttattat caggatttgg gatatgatta
    15061 ggaaatcggc catctggttc caaaaattgg gaacctgttg aatccgcacc taagctattc
    15121 aatacttttt cagcaaagaa tccaccagca ccatttccgg catcaacaat gattttccaa
    15181 ccatttagtg gtttgctttc tagtgttcca atcccctttt taatttttgt gactaaatct
    15241 tgcgcataag gtgtcaaaag gtctgctttt tctacgcttc cagtttgttc gctttttatt
    15301 ttttgctgct gtgataggat aaaagcaata tcttcttttt ctgctcctcc ttctgaggta
    15361 aagaatttta ttccattata ataataggga agatggctag cagtcagcat aaccgcagca
    15421 tcgcacgaga actgttcaaa ttgagtggac ataaacatag caggcgtagt agctaattcg
    15481 aaatcgatga catgtatccc ttgttctttc agactttgga tcaacgcctc tttcagtgct
    15541 ggtccgctta tcctactgtc atgaccaatc ccaatcgtcc attttttatt tggatcggtt
    15601 gatttcttct ttttctttat ccagtttgct agtccccagc cgatcttttt taccatgtct
    15661 gtcgtcaagt ttacttcata tccttcaact ggtaatgcga ttcctcgaat atccgatcca
    15721 ttttgcagcg ctttcatatc tttcatataa aagtcctcct catttattcc ctcatttttt
    15781 ccagcttttc tgcaaatcgc tgtgcttctt cttctgagcg gcaaataata atgaatgtat
    15841 ctacaccggc catcgttccg acaacttcag ggtaagccac ttcatctagc caattcgtta
    15901 ccacatcggc accatttcgt tcagtcaata cgataaccat gaactgaaca gactggatac
    15961 gcaaaacctc gcgtctaatt gctgtacgca aacgttcttc atttaaagaa acagatggct
    16021 ggttaaataa tgtatagcgt actattttgt tttcattttg actttttact aacttcattt
    16081 ctcgaatgtc tcgagaaatc gttgcttgag tcacatcggc gcctgcttca tgcagccgcg
    16141 caagcagctc ttcctgtgtc ccaataggtt gttctgtaat gatctggcgg atcagtgctt
    16201 gtcgctgttt ctttttcata ttagacacct ctcaatgttg attatagcaa aagataagta
    16261 atatttcttc atattttggt attttttact tttatttttt gttttcattc aggttgctaa
    16321 cctttttcta ttttaacacg caaaatcgtc ttcatttttt caggtgctga ttttcttgga
    16381 tcgctgatgt aaatctcttt gtgttcttta gaagctcttt catagccttc ttttgcgatg
    16441 aatgcttcta atttctcaaa cgttgctggt tcttcggcat agggcccgac gtgtaagatt
    16501 tgggcacaaa gaccttcttc catctttttg aatactactt gttctttcaa gtcttccgga
    16561 agtttgctag aacgctgaat ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt
    16621 cggatcatca attgataaga aaaatgttct tttttcatca ctggttcatg caaatacttt
    16681 tcttgcagtc cccattgtcc ttccagtgga tagacagtat acatctgata atcaggaatc
    16741 agccaatttt ttttaggcga catgcgtatt gcatagctga ctgcatatag acaggctact
    16801 cttcgtttaa agtcttcctg attcgggtcg ccgatccctc tgatgctaaa gaatgatttt
    16861 ttaggaacct ctagtatttg cgggcttttt ttcatactat agctcttgtc tttacgccat
    16921 tcaatttttt ccatttttct tcctccgcga aattccattt agttatttat tatcatacct
    16981 ctttttgagt gacttggcta atcgtgttga cactattttt cagtttgtgc tagaataagt
    17041 gaagtaaata aatcgtacag ataacacgtt tcggcgtgtc gagagagagg cggtttctat
    17101 ttttataata gaaatcactc tcttttttta cgaaattttt tagaggtgat gtaaatgaca
    17161 aaacaaaaac agctgctttt agtacctgca gtaattgcaa cagggatgat gtcttttgcg
    17221 ggcgtattga tcgagacggc catgaatgta actttcccaa ctcttatcag acagtttgat
    17281 ttaacgactg cacaagtcca atgggtaaca acgatttatc ttttgatgat ttcgatcatt
    17341 gttccgctgt ctacttattt gaatcgcaat tttagtctca aaaaactctt tttagcttca
    17401 aatctgcttt tcttggcagg tataatgatc gatttcttct ctcctacctt catggtccta
    17461 ttgtttggtc gattgctcca aggtgcagcg acaggtatcg ctcttccgtt gatgttccat
    17521 attatcttga catttgcccc cctcaatcga cgaggcgcga tgatgggtgt cggtactttg
    17581 acaacggcca ttgctccagc aattggcccc acttatggag gcattatgac ttcacatttt
    17641 acatggaatc atatcttcct gtttcttgtg cccgttttgt ttctttcttt gattatcggc
    17701 tggtttgcta ttcccgagat ggcagtacaa aaaagtggtt atttggattg gataagcgtt
    17761 ttaggaattt gtcttttgtt tagtggaagt ctaacatttt tgagtacgtt gggaacagtt
    17821 tccagctgga ttgctttaat catcggcggt attggctggt tcctttttta ctatcgaagt
    17881 aaaaaagtgg agcatccact gatccgactg tctattcttt caaataaaac attccgcttg
    17941 tttttgattg gcttcttagt ttatcagttt ttattacttg gtgtatcttt tgtcttgccg
    18001 aatttccttc agatcgttca aggaagtagt ccttttgtcg ctggattagc gatgcttccc
    18061 ggcgcagcgg ttggtgcttt gctttctcct ttttccggga aaatgctgga tcaatatggt
    18121 ccgaagaagc caatttttgc tgggctgatt ctttctttga ttggttggct agcattattc
    18181 ctactcatag gacacgcttc agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc
    18241 ggtatcggat tatcttacag caatatgatg accaccggaa tgaatgcact gaatgaagaa
    18301 ctacaaggag acgggaacgc aatgttcaat accttgcagc agttttctgg cgctgtggct
    18361 acttcattag tagcggtcat tatcaattac gtccagcatc atacttccca taactatgaa
    18421 gtgtctacta cattaggatc aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta
    18481 agctttgctc gattcgccta ttatctgttc tttgctaaaa aagcgtagat tccatatacg
    18541 gcaggtgttg catctgccgt ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt
    18601 ttatggaaaa aattgccatt atcggtggcg ggatcattgg tatgacccta gccagttact
    18661 tagatacatc aaaattcaat gttgctttat ttgatgaagg gacaggacag gcaactaaag
    18721 ctagcgctgg gattatctcc ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc
    18781 tggctaaaga cggcgctgct tttttcccta aacttataaa agatttccaa cttgatcaaa
    18841 cgatttacgc acaatcaggg acacttttgc ttcgacctga aaaagattta gctgaattgg
    18901 ctgttcttgc cgagaaacga aaagaagaag cgccagaaat tggaaacatc gaatggcttt
    18961 cacctgaaca gacatctcaa gcacttccgt tattgaaacc agcagtttca ctgcgtatca
    19021 gtggtggtgg tcgtctggat ggaaaagcat atttagaaca tcttggtcag cgtttagcac
    19081 aaaaacaaat ggagcagatc caggcaaaag tcactttgca taagaagagc gattcttggc
    19141 tcatcaaggg accgtttgga gaatacacag ctgatcgtgt aattttgaca cctggcccgg
    19201 cacttaagcc tttattacaa gaattaggct atgctgcaga tatacgtccg caaaaagggc
    19261 agctcttagc atttcagaca tcttataaga tgagtcaaga ctggcctgtt gctatgcttg
    19321 acggagaagc tgatttcatt cctttcatgg atggaaagat ccttttaggt gctacccatg
    19381 aaaacgatca aggctgggat ttggctccta ctagcgaagc ttataaggga ttgactaaga
    19441 gtacagaaca gttcttgact gatccagatc agataaaaaa ctggccatac gagttccgtg
    19501 taggtacccg tgcttatacg tcagattttt ccccattttt cggcgtactg ccagatgatc
    19561 caagcttact ggctgctagc ggtctaggct cttctggctt gaccacaggt ccttatatcg
    19621 gctatctatt agccaacttc ttaaatactg gtcattggcc tacggaagat tatcaaaagg
    19681 acatctccac ctatattacg aaaaatccat catagaaaaa cagctttttc aagtaaggat
    19741 cccgagcgat attgcgcatg gattacttct tgaaaaagct gttattttag ttcaatttct
    19801 gaatcaaatt ctccaaactt tcttttgctt catctggcaa gagatctttt cctatctgag
    19861 cttcaatgaa tcgtaaacga atatcaagtc gttgtttcac cagattttta taggacgtat
    19921 cctcaaaaga aacaggaaaa tctggtatag gtaaatagtg gatgtcttca aatggaccaa
    19981 acgacttgaa ttctgccgtt tgttgcacga ttctttcaac gattgacaat gtcgtttcag
    20041 gtttgatttt tttatcatcg aaatatccag tattcaggac atagcaggct gtttgctgtt
    20101 tcaaaaacaa ctctcgaaaa gcttgatagt cttccctaag aggatagaca cggaaaggat
    20161 tagcaaatgg ttcaatgact agctcatcta gctgtctttt tgtagtgata ttttccgcgt
    20221 ctgatcgttt tgttgctaat gttgcaccaa aagacgctgc taaaactggg tcattgatct
    20281 tgataattgg cggaagcgaa tcatctttca tgatccaaaa aacagcatcg atcttttcgc
    20341 taagatgatc gactcggtcg ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt
    20401 ttcgtatatc tcctgttact aggacttttt ctcctttact tgtacatgtc acgcccacat
    20461 tctgacaagt aaggaaagaa gaaatggccg gatcatcaga cggatagtct tgtgttttat
    20521 caaagtaaga aggttccaag gcagtagcaa aaccattttt tcgagaaata acgaaagcat
    20581 cgtcatgcaa gatcgaaaca gagtgacctt ccgtatgttt tgctaaggta atcgttgatt
    20641 ttccagaacc ggacaaccca aatacagcca tcgtataggt ctggtcatta ctagcatatt
    20701 gtttcatccc accgtggcag gcaataaagc cattgcgatt tcccaatgcc caagcaagcg
    20761 tcaaagtcgc tttttttaat tctccaaagt aacgtaatcc tagcacagcc gctgtattat
    20821 ggagagcatc aaacaaaatg agtccatttg gaaatgtttc atgtgtccat tcgggatcac
    20881 aaaagatata aagatctccc tcatcaaagg aaacagactt ttgataagtc tctaagatct
    20941 tcgaattcgc tggctgaaaa ttcagcaaat aagaatacaa attgttctca aatccttctg
    21001 gaatcagcaa atgactttgc acagaaaatt cttcagatag gccaacgatt acttttgtat
    21061 ggtaaaactg gcgcttgcga ctttgataca cagcttctct taaaattttc tcgtatttgc
    21121 tttgttcctt gtctaagtcc tcaaagatcc gccgagcact tgctgttctt cccacaatgc
    21181 ttccatcatt gaatactagt gtcctaacat cagctggtaa atctagttct tcagcatgtg
    21241 agattgttaa atctgtgatg atcgttcctg gactcccttt tgctagttga taagctgtct
    21301 tcctatcaaa aacttcatga acattattac cgtaaaatgt cgtttctaca atcgatcgaa
    21361 tactggataa cagtggattt ccacttgtga tttcgtttaa ttcgagactc tcttttgtac
    21421 tcatggctcc tcctcattcc ctaaacaaaa aaacgttttc tttattatac attattcgag
    21481 tttttatttg tactgctgcg ctgtgggaag caaacgagtt atttttcaca caaaaaaacc
    21541 gagaacttga ataaacaagt ttctcggttt catcattttc cttttatcgg cttagtacca
    21601 gccgtttgct aaccagaatg ttttagcagc atcccatgaa ccataacgag atgttacata
    21661 ttgttctgct acacgttctt gattggcagc tgaatagtcg ccgtttaagt atgaagcatc
    21721 taattgataa cggccgatgt aacgtccgtt agtagctgtg tatgaaccgc ctgattcttt
    21781 ttgtgcgatc cattcttttg cagagcttga agttggcgct gttgcagcag ctggggcagc
    21841 aggagctact tcaactgttt caacaggtgc aacgtatgta tgttcttgcg ttgctgtttc
    21901 agttgtagat gctgtatttt ctgttgtttt gttttctgtt gttgctgaag aatcatttga
    21961 agttgggatt gttaattgtt caccaacata aatgcggttg atgtttgcga tcgaattttt
    22021 ttcagcaatt gcatcgatca ttgagttgtc tcctgcaaat ttttgtgaga tcttagataa
    22081 tgaatcacct gattttactg tataaacttc atcagcgtga gcatttgtag ttcctacaaa
    22141 cattgcagta gcaccagcag caaaagttgt acctaaaaca attttcttaa atgaattcat
    22201 attaaactcc tttttttctc cgtt
//
LOCUS       DAZULG010000043        21996 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000043 DAZULG010000000
VERSION     DAZULG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.043"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="ACG9CY_001410"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="ACG9CY_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS4937926.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="ACG9CY_001411"
     CDS             complement(2017..2919)
                     /locus_tag="ACG9CY_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4937927.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="ACG9CY_001412"
     CDS             complement(2916..3608)
                     /locus_tag="ACG9CY_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS4937928.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="ACG9CY_001413"
     CDS             complement(3651..4292)
                     /locus_tag="ACG9CY_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS4937929.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4330..4644)
                     /locus_tag="ACG9CY_001414"
     CDS             complement(4330..4644)
                     /locus_tag="ACG9CY_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937930.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACG9CY_001415"
     CDS             complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACG9CY_001415"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS4937931.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACG9CY_001416"
     CDS             complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACG9CY_001416"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS4937932.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5981..6913)
                     /locus_tag="ACG9CY_001417"
     CDS             complement(5981..6913)
                     /locus_tag="ACG9CY_001417"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS4937933.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6910..7686)
                     /locus_tag="ACG9CY_001418"
     CDS             complement(6910..7686)
                     /locus_tag="ACG9CY_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS4937934.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACG9CY_001419"
     CDS             complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACG9CY_001419"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS4937935.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10930..12006)
                     /locus_tag="ACG9CY_001420"
     CDS             complement(10930..12006)
                     /locus_tag="ACG9CY_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS4937936.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12011..13291)
                     /locus_tag="ACG9CY_001421"
     CDS             complement(12011..13291)
                     /locus_tag="ACG9CY_001421"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS4937937.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13310..14236)
                     /locus_tag="ACG9CY_001422"
     CDS             complement(13310..14236)
                     /locus_tag="ACG9CY_001422"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS4937938.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14249..15532)
                     /locus_tag="ACG9CY_001423"
     CDS             complement(14249..15532)
                     /locus_tag="ACG9CY_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS4937939.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACG9CY_001424"
     CDS             complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACG9CY_001424"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS4937940.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16320..17231)
                     /locus_tag="ACG9CY_001425"
     CDS             complement(16320..17231)
                     /locus_tag="ACG9CY_001425"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4937941.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACG9CY_001426"
     CDS             complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACG9CY_001426"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS4937942.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17697..18395)
                     /locus_tag="ACG9CY_001427"
     CDS             complement(17697..18395)
                     /locus_tag="ACG9CY_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS4937943.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18382..18768)
                     /locus_tag="ACG9CY_001428"
     CDS             complement(18382..18768)
                     /locus_tag="ACG9CY_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937944.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18915..20585)
                     /locus_tag="ACG9CY_001429"
     CDS             complement(18915..20585)
                     /locus_tag="ACG9CY_001429"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS4937945.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20768..21001
                     /locus_tag="ACG9CY_001430"
     CDS             20768..21001
                     /locus_tag="ACG9CY_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4937946.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21100..21531)
                     /locus_tag="ACG9CY_001431"
     CDS             complement(21100..21531)
                     /locus_tag="ACG9CY_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS4937947.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21610..>21996
                     /locus_tag="ACG9CY_001432"
     CDS             21610..>21996
                     /locus_tag="ACG9CY_001432"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS4937948.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHK"
BASE COUNT         6213 a         4919 c         3887 g         6977 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
     4501 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
     4561 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
     4621 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
     4681 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
     4741 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
     4801 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     4861 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     4921 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     4981 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     5041 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     5101 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     5161 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     5221 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     5281 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     5341 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     5401 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     5461 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     5521 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     5581 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     5641 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     5701 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     5761 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     5821 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     5881 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     5941 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     6001 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     6061 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     6121 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     6181 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     6241 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     6301 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     6361 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     6421 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     6481 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     6541 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     6601 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     6661 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     6721 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     6781 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     6841 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     6901 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     6961 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     7021 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     7081 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     7141 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     7201 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     7261 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     7321 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     7381 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     7441 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     7501 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     7561 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     7621 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     7681 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     7741 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     7801 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     7861 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     7921 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     7981 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     8041 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     8101 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     8161 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     8221 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     8281 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     8341 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     8401 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     8461 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     8521 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     8581 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     8641 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     8701 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     8761 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     8821 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     8881 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     8941 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     9001 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     9061 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     9121 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     9181 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     9241 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     9301 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     9361 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     9421 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     9481 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     9541 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     9601 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     9661 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     9721 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     9781 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     9841 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     9901 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     9961 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
    10021 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
    10081 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
    10141 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
    10201 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
    10261 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
    10321 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
    10381 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
    10441 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
    10501 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
    10561 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
    10621 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
    10681 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
    10741 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
    10801 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
    10861 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
    10921 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
    10981 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
    11041 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
    11101 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
    11161 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
    11221 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
    11281 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
    11341 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
    11401 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
    11461 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
    11521 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
    11581 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
    11641 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
    11701 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
    11761 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
    11821 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
    11881 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
    11941 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
    12001 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
    12061 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
    12121 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
    12181 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
    12241 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
    12301 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
    12361 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
    12421 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
    12481 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
    12541 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
    12601 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
    12661 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
    12721 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
    12781 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
    12841 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
    12901 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
    12961 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
    13021 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
    13081 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
    13141 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
    13201 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
    13261 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
    13321 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
    13381 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
    13441 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
    13501 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
    13561 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
    13621 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
    13681 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
    13741 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
    13801 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    13861 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    13921 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    13981 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    14041 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    14101 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    14161 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    14221 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    14281 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    14341 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    14401 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    14461 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    14521 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    14581 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    14641 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    14701 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    14761 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    14821 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    14881 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    14941 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    15001 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    15061 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    15121 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    15181 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    15241 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    15301 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    15361 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    15421 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    15481 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    15541 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    15601 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    15661 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    15721 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    15781 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    15841 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    15901 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    15961 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    16021 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    16081 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    16141 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    16201 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    16261 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    16321 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    16381 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    16441 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    16501 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    16561 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    16621 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    16681 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    16741 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    16801 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    16861 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    16921 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    16981 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    17041 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    17101 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    17161 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    17221 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    17281 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    17341 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    17401 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    17461 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    17521 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    17581 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    17641 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    17701 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    17761 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    17821 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    17881 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    17941 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    18001 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    18061 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    18121 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    18181 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    18241 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    18301 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    18361 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    18421 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    18481 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    18541 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    18601 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    18661 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    18721 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    18781 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    18841 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    18901 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    18961 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    19021 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    19081 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    19141 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    19201 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    19261 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    19321 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    19381 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    19441 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    19501 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    19561 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    19621 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    19681 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    19741 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    19801 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    19861 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    19921 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    19981 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    20041 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    20101 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    20161 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    20221 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    20281 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    20341 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    20401 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    20461 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    20521 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    20581 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    20641 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    20701 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    20761 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    20821 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    20881 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    20941 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    21001 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    21061 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    21121 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    21181 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    21241 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    21301 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    21361 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    21421 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    21481 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    21541 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    21601 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    21661 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    21721 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    21781 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    21841 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    21901 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    21961 ggagcagata atcttgcacg tcaggctctc cataaa
//
LOCUS       DAZULG010000044        21337 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000044 DAZULG010000000
VERSION     DAZULG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.045"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            263..964
                     /locus_tag="ACG9CY_001433"
     CDS             263..964
                     /locus_tag="ACG9CY_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4937949.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1573..2589)
                     /gene="tsaD"
                     /locus_tag="ACG9CY_001434"
     CDS             complement(1573..2589)
                     /gene="tsaD"
                     /locus_tag="ACG9CY_001434"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS4937950.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2586..3050)
                     /gene="rimI"
                     /locus_tag="ACG9CY_001435"
     CDS             complement(2586..3050)
                     /gene="rimI"
                     /locus_tag="ACG9CY_001435"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4937951.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3057..3599)
                     /gene="rimI"
                     /locus_tag="ACG9CY_001436"
     CDS             complement(3057..3599)
                     /gene="rimI"
                     /locus_tag="ACG9CY_001436"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4937952.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3583..4305)
                     /gene="tsaB"
                     /locus_tag="ACG9CY_001437"
     CDS             complement(3583..4305)
                     /gene="tsaB"
                     /locus_tag="ACG9CY_001437"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS4937953.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4496..5476)
                     /locus_tag="ACG9CY_001438"
     CDS             complement(4496..5476)
                     /locus_tag="ACG9CY_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4937954.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5500..6984)
                     /gene="galT"
                     /locus_tag="ACG9CY_001439"
     CDS             complement(5500..6984)
                     /gene="galT"
                     /locus_tag="ACG9CY_001439"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS4937955.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6996..7985)
                     /gene="galE"
                     /locus_tag="ACG9CY_001440"
     CDS             complement(6996..7985)
                     /gene="galE"
                     /locus_tag="ACG9CY_001440"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS4937956.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8233..8400
                     /locus_tag="ACG9CY_001441"
     CDS             8233..8400
                     /locus_tag="ACG9CY_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS4937957.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8462..10273)
                     /locus_tag="ACG9CY_001442"
     CDS             complement(8462..10273)
                     /locus_tag="ACG9CY_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS4937958.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10270..10635)
                     /locus_tag="ACG9CY_001443"
     CDS             complement(10270..10635)
                     /locus_tag="ACG9CY_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS4937959.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10798..11193)
                     /locus_tag="ACG9CY_001444"
     CDS             complement(10798..11193)
                     /locus_tag="ACG9CY_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4937960.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11211..12173)
                     /locus_tag="ACG9CY_001445"
     CDS             complement(11211..12173)
                     /locus_tag="ACG9CY_001445"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS4937961.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12173..12385)
                     /locus_tag="ACG9CY_001446"
     CDS             complement(12173..12385)
                     /locus_tag="ACG9CY_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS4937962.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12406..13104)
                     /locus_tag="ACG9CY_001447"
     CDS             complement(12406..13104)
                     /locus_tag="ACG9CY_001447"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4937963.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13124..13666)
                     /locus_tag="ACG9CY_001448"
     CDS             complement(13124..13666)
                     /locus_tag="ACG9CY_001448"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS4937964.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13798..14802)
                     /locus_tag="ACG9CY_001449"
     CDS             complement(13798..14802)
                     /locus_tag="ACG9CY_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4937965.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14799..15788)
                     /locus_tag="ACG9CY_001450"
     CDS             complement(14799..15788)
                     /locus_tag="ACG9CY_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4937966.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15785..16591)
                     /locus_tag="ACG9CY_001451"
     CDS             complement(15785..16591)
                     /locus_tag="ACG9CY_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4937967.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16757..17713
                     /locus_tag="ACG9CY_001452"
     CDS             16757..17713
                     /locus_tag="ACG9CY_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4937968.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17790..18308)
                     /locus_tag="ACG9CY_001453"
     CDS             complement(17790..18308)
                     /locus_tag="ACG9CY_001453"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4937969.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18396..18545)
                     /gene="rpmG"
                     /locus_tag="ACG9CY_001454"
     CDS             complement(18396..18545)
                     /gene="rpmG"
                     /locus_tag="ACG9CY_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4937970.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18773..19219
                     /locus_tag="ACG9CY_001455"
     CDS             18773..19219
                     /locus_tag="ACG9CY_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS4937971.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19414..21309)
                     /gene="asnB"
                     /locus_tag="ACG9CY_001456"
     CDS             complement(19414..21309)
                     /gene="asnB"
                     /locus_tag="ACG9CY_001456"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS4937972.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6419 a         4521 c         3678 g         6719 t
ORIGIN      
        1 tgaaaaaatt tttttgaata aattaaaaaa ataaattata ttatcgttgt tagcgtttac
       61 tatatgtatt attgttgttt tttatacaca aatcattcgc ttcaaaacaa attattcaat
      121 accattgtat attttttaca taataacaaa ttatatttgt aaagtattaa aacttgtttc
      181 taatgttggt atgttaaaac tttacaaatt ctttaaaaaa agtgacaata aatatagtga
      241 acaataagga gaggactttt taatgcgtgt taataaaatc gattttcact tttcaaaact
      301 cagatatcaa cctgattttg aacatttctc ggatgaagag ttccaacaga tcaaggaaca
      361 tactgttctt cgttcttata aaaaaggaca gatccttttc gacgatggtg atcccagaca
      421 acggttgtat atcttgacca aaggactcgt tcgtttagaa cgttatgatg aaagtgcaac
      481 ttattactac tatgactatg tgaatacaaa taaccttttt cctatgtcag gaatgtttgg
      541 agaagagacc tacacttata cagctcaagc gatgactgat attgaaacat tctaccttcc
      601 tgtttcctac tatgaaaaat tagcacgaaa aaatcccgaa cagttgatct atatgataca
      661 aaaaatttct aaagtagttg aaatacatga attgcgtatc caaatcggat tgacctctag
      721 cgcctttgac cgggtaaaac aaaatttatt cattttgaaa gaagaattgg gcgtaccaac
      781 aaaagatggt catatcacca ttccttatcc gatcacctta aaagaattag cagtgaatag
      841 cggcacgaca cgtgaaactg ctggacaagt aatcaaacaa ctacgtgatt ccgggctgtt
      901 agattatgac aaaaagcgtt ttactttctg taagacagct actactccag ctatgagaga
      961 ataacgcgaa caaaaataag cagtgaccct gataaatatc agggtcactg cttattttcc
     1021 tccctaaaac ttctgaaaga tccggcagtt ttagatcact ggacaagact caaagatttt
     1081 ctatgtgtgt ttcagttatg taatattcac tgtttgcaaa aaatactgtt agattaacta
     1141 gatgaagcgt cttgtcctca tccgatctag attattcgat attcctcgac aataaacgat
     1201 tactcagtga gggcaatcag aggaacggat aatgataaat aagctgtaaa aaatagcaaa
     1261 ataacaataa tgattaaaag tatgtaaaaa atagggcttt gacaaaaacg tgtcacagcc
     1321 ctatttcgat taaacggtgt gtacctgtca actcttcggt cacaagtcat aatgttcata
     1381 aacatgagga gctgtttttt gaaattgttc cttgtgaatc agagttaaac gacaggctta
     1441 cgaactcttg attaaacggc gttcaaaagc ttgcatacgc ggtaataaga acagccaggt
     1501 caaaaacatg agaagccgtt tttcctgggc tgttcttatt acttgatgca gggcagcttt
     1561 ttcacaacct ctttattcag ataaaaccaa gcttggatca gcattcaact gatagctagc
     1621 aaaatttctt ttttgcattt ccacatgtgc tgctgctcca atcattgcag cattatctcc
     1681 acaaagtcga agagggggaa tcactagttc tacctcaggg agtttagctg ccaaagcatc
     1741 ctgcaatcct tcacgcaacc cttgattggc cgctacacct ccagctataa tcagttgctt
     1801 gactggataa ttttgacaag ctcgaagtgt tttagtaatc aatacatcga tcacacttgc
     1861 ttgaaagcta gctgctaaat catttttgtc tagatcttct ccacgctgtt gtgcattatg
     1921 caccaaattg ataaatgcac tttttaatcc gctaaaactg aaatcataat tatcttcatg
     1981 gatcatcgct cgcggaaaat ggtaattatc ttttccctga tgagctagct gatcgatttc
     2041 ttttccgctt gggtaagaaa gacctaatac tcttccaact ttgtcataag cttctcctgc
     2101 tgcgtcatct cttgtctccc caatgatttc atatgatcca tcttcttgca tatagactaa
     2161 ttctgtatgt cctccgctaa caagaagtgc catcaatgga aactggaatg gtttaaccag
     2221 acgggctgca tagatatgcc ctgccatatg gtttacagga ataagcggca attggtgtgc
     2281 ccaagcaaaa gccttagctg cagaaatacc aatcagcaac gagccgacaa gtcctggtcc
     2341 ataagtgaca gcgactgccg ataaatcttc tgccgacaca ccagcttcta ccagtgcatc
     2401 ttccaagcat aaggtgattt gttctacgtg atggcggcta gctacttcag gtactacgcc
     2461 accaaaacgt ttatgactgt ttatttgaga agcaacgatg ttagataaga tctctgttcc
     2521 atttctaacg accgctacac tcgtttcatc acaacttgat tcaatcgcca gaatcaattc
     2581 ttcattcatt tcatcgtctc cgtttttaac tttctgctca taatgatcgc atcttccgtc
     2641 ggattgcgat aatatgattt ccgtttacct aatatacgaa acttttctga ttcataaagt
     2701 tttcttgctg cttcattaga tagacgtact tctaaaaaaa catgcagcga atcttttatt
     2761 tgttccgtaa tcagtaattg taacaaatgc cgtgcatgcc cttttccttg ttctgaaact
     2821 gcaacagcaa tatttgtgat ctcgacttca tctattactt tcgagtatcc aataaagcca
     2881 cagatctggt tatcttcagc taaaagcaga taatcagagt gaggctgttg gatgtctgcc
     2941 aaaaactgca ttttcgtcca aggtgaacca tgtggataag cattttcgct aatcatccat
     3001 acgcattctg ccaagtacgc ttcatcaaaa tctttttttc tgcaaatcaa ctaatctcac
     3061 tcattttcta aaagttttat catatccaac gcatcttctt ttgtttcagt gtaatatctt
     3121 tttttgatcg ttgttgcttg aaaccccaat ttccgataaa gtctttgtgc atcttgatta
     3181 ctcatacgca cttctaatga catcgtctca cagcgattca ttatagcaaa gttctcgatt
     3241 tcgtcaataa gcagcgaacc gattcgttta cgctgatatg ccggtcgaac ggcaatattc
     3301 gtgatatggc aatctaaacc gaacgctctt gaaccgataa aaccaatcat tcttccatta
     3361 tcaaagatac ctagatatag atgaggaagc ggtgacttca attctgatat aaaggcactt
     3421 tttgtccatg gcaattcgcc attatagact tctcgttcga catccagcag ctctttgata
     3481 tcttctgtag tcaattctct tagtacgtaa cgacgatcga tttgttcgac tgttttttct
     3541 gtataaatca ttttatcttg aaaaaagctt ctggcatacg ctctaaattt tttcaacata
     3601 actttcgtct ccaggtgtgt gcgtctccaa ccatttttct tctgcttcta ctcgttttaa
     3661 atagcgagga agaaaagctt gtttctctgc aactggttca actaaagcac ctaattgagc
     3721 cacagttaca ccacttggaa tatcccatgc tggaactgta ttaatcatcg cactaggcaa
     3781 tatttccttt atttcagaga agaatttgtg acaatcactc ccgacgaaat aaatagcttc
     3841 attcagcttt tgtagttgtg tgaacaaatc agaagcagaa atatgtacat cttcaagtac
     3901 agtttctaat ctattttctt tccagcggta tgccccagcg tatacatttt ttcttcgagc
     3961 atcaaacatg ggcacaataa tattatccac accgatgcaa ttagccgcga tggttttcag
     4021 actagatact cctactagct ctttatctaa tgtataagca atcgtttttg ctgtcgttac
     4081 acctatacga agccctgtat aagaaccggg accatcagaa accgcgattc ggtcaatgtc
     4141 ttttggactt atttgaagat ctgcaaacaa ttggtcgatt gcaggcatca gtgttacact
     4201 gtggttgcgt ttgatatttg tttgtatttg tccaagaatt tgttcgtctt ctaccacacc
     4261 gatcgccaaa gtttggtttg cagtatctat tcctaatgta atcatatatt tgatcctttc
     4321 tgctatgaca ttctcgcctt ccattgtagc atactttttt attctgagga tgaaaatacc
     4381 aaaggcaaaa ctagtacaat aatacatgat tatctgctgg cgggcaaatt ttgccccttc
     4441 ctgaaacaca aaaaagctgc ggtatcactc cattttccca cagcttttcc atcttttatt
     4501 tcgttgattc tcgaataatc aactctgttt ccacaataat ctttcttgaa accggtgctt
     4561 tggaattact taattgaaga atcgtctcca ctgctagctc tcccatccat tcggtataaa
     4621 cttttacagt agaaagcgag ggcgttacat atttcgctac actgacatca ttgaagccca
     4681 tcacagaaat atcttctggt accactaaac ctttttcttg aatcgctttc agtgcgccaa
     4741 tcgctaatgc atcattagct gcaaaaagcg catcaggaac atgcggattc gtttttaaat
     4801 attgcttcac tgcttgatat cctgactcga cagaaaaatc tgcttcgata attggacctt
     4861 cgtcgtagcc tttttctgcc aaaacttgtt gaaatgccat aattctggga tcttttagcc
     4921 gctgatggtt tttcttcgta tattctgttc cagcaagcat agcgatatgt tgcgctttat
     4981 gggagacaaa gtgttcaatg actttttgaa cacttgactt aaagtcgaca actagagagt
     5041 cgattccttg agaagtgccg tcagaatcta ccaaaagcaa ggcattttgg gatcctttca
     5101 tttctaataa ctgagcttcg tcgaattttc ctagtgcgat cgttccatct gccggaagat
     5161 cactaatgga atcccacgtt tctttcaata aagtaacgtt tgcttcttct gccttttttt
     5221 cgattcctag gcgaatagat aaataataaa gatcttcaag ctcttccgta tcgtcgtacc
     5281 actggatcag gcgaagcgtt tgcttagtat ttgctgcatt tttacgatgt ttcgtataat
     5341 ttaagcgttc tgctgcttca aaaatttttt gctttgtggc taagccaacg gaaagatcag
     5401 gatcatagtt gagaactctt gaaacagtag caggagaaac tcctgccaac tgcgcaatgt
     5461 ctttaattgt tgccatttgt ctctcctcct tttttacgtt tataaaatcg cagtaaatcg
     5521 ttcaaacgct gcctgtcctt tttcatctcg cttgaatact ccagcgtctt ccagtacacg
     5581 tgcaaaaacc tgacctacag atttttgtac gacatcggtg accgtatctt tcgtcaaatc
     5641 aggatgttct ttttttaatt gttctgccca cggacgatga tattcagcca tctggttttc
     5701 ttctcctaat aagaaatgct ctacttctac tagttctggt ttcaaacgag gtggtaaaac
     5761 agcaagcccc attacttcga tcaacccaat gttctccttt ttgatatgtt ggacatcttt
     5821 atgtggatga aaaatcccat caggatgttc ttctgaaaca ttgttgtctc gaaggacaag
     5881 gtccaactca aagtaattgt cccttcttcg tgcaattggc gtaatcgtat ggtgtggtgt
     5941 accatctgct gaaaaagctc tgatagatac tgtttcatcc gaatatgctt tccatttttc
     6001 caatactgag ttagctgctt caataagcaa gtttttctct gttgattgca aacggatcac
     6061 agacatcggc catttcacga ctcctgcgat gacttgcgga taatcttcta gtttgataag
     6121 tttttctatc ggtgcttttg ccataggaaa ttcatgacgt cctgcctgat aatgatcatg
     6181 agaaagaatc gatcctccaa caattggtaa gtcagcattc gaaccaacaa agtaatgagg
     6241 caagacttca gtaatccgaa gcagtctagc aaatgtttcc tttgtgattt tcatcgggcg
     6301 gtgttgttct gataaaatga tcgaatgttc attgtaataa gcataagggg aatattggaa
     6361 tccccagctt tcaccgtcta gattcattcg aataatccgg tgattggtgc gggctggata
     6421 gttcaagcga cctctatatc cttcgttttc catgcaaagc atgcaggtag gatagtctac
     6481 ttttaccgcc tcgcgttctg ctgcgatttg ttttggatct ttttctggct tagataaatt
     6541 gattgtgatt tccagttcac catactccga ctctgccgga aatttgatgt tttttgcgat
     6601 ggctcttgtt ttgatgtagt catttttctt acttaaatta aagaaatact cagttgcttc
     6661 ctctgggctt ttcgaatagt gctgtgcaaa gaatgcattg acgactgaag gtggcggtgt
     6721 gagaaaatcc attaattgcg cttcaaaaat ctcttgttct gctggcaatt ctgaaagaac
     6781 accattttct cttgccttag cgacaagctg atccaataag tctacagatt cttccttaac
     6841 tggtcttact tcaatgcttt ctagagcatc ttctcctacc atccctaaaa gacgattctg
     6901 taaatataag cggtccattt ccatccatcc gccagcttcg atcgcaagtg tggcaaaatc
     6961 acaaatcgtt tgactgattg acatatctca ttcccctatt cttcataacc atttggatgg
     7021 ctgacatgcc aatcccatgc tgttttgata atcgctttga tatctgtgta ttcaggtttc
     7081 cagcccagaa tctctctcgc tttttcactt gaagcgacta atcggctagg atctccagct
     7141 cttcttggtg ctatttctgc tggaatttct tttcctgtca cttctctagc tgcttctagc
     7201 atttccttga ctgagtatcc tttgctgctg cctaaattga agaagttgct ttcatttcct
     7261 tctttcaagt attccaaagc taaaatatgt gcagcaatca aatcttctac ttgaacataa
     7321 tctctgatac atgttccatc tggtgtatca taatcgtctc cataaacagc caaggctttt
     7381 ctttgaccaa gagctacttg taaaatgatt gggacaagat gcgtttctgg tgtatgatct
     7441 tctccgatag atgcatctgc ttttgctccg gctacattga agtagcgcaa agcgacatag
     7501 cgcatgccat atgcttggtc acaccacttc atcatttttt ccatcatcaa tttactttca
     7561 ccatacggat ttttaggatt agctggtgta tcttctgtga ttggagactc tttcggctct
     7621 ccataagttg ctgcagtaga agagaatacg attttattca cgttgaactc gtgcatgact
     7681 tcaagtaaga tttgcatacc gtatacgttg ttgttgaaat acatcaatgg tttttcgaca
     7741 gattctccta ctaaagagct tgcagcaaaa tggattactc cttcaatcgg ttccttttca
     7801 aatacggaac gtagaaattc tttatcccgg atgtctcctt cataaaaatg agcatctgga
     7861 tgtactgctt gtttgtgacc tgtgagcaaa ttatctacca cagctacttg ataccccttt
     7921 tgaactagct ggtctacagc gtgcgaaccg atataaccgg ctccgcctag tactaaaatt
     7981 gtcataaaaa acactccttt gattagtaaa attgtgtttg ttgattctat ataatttaaa
     8041 aaacgaagtt tacttttatg gtgaaaacaa acaaagtaaa atttcgtaaa ttcatatggt
     8101 aaatttataa taaaacattt agtaaacatt ttcaagggtt ttttgcaaag gtttacaaac
     8161 aaacgtaaaa ctagcctttt tttctctttt gttccataat agatataaca aataataaat
     8221 gaggtggatt caatgaaaaa atttgtatct ggaatattag taggaagtct ggcaacagcc
     8281 gctgccgttg ctggtttggt tgcttctgtg aaaaaaacag tgatcgatcc aattgacgaa
     8341 aaagaagcaa tgatcgaaga aaaccgtaaa aaagcaatgc gtaaacgcgt ttctcgttaa
     8401 taattattta aaaatacgca tgaagacttt ctaaaaaaca gataagtcat catgcgtatt
     8461 tttatttgtc agtcaatata agttgtgcta ttttatctgc ctgtctcaga ttccatggat
     8521 ctgtataagg tctagctact gctgcagtta gccaatccac accttcaaca ggtattccat
     8581 agcaataaaa gtcagactgg atttcccctg aagcatcaat cacgcgattc gtacctcgct
     8641 caacatctat agcttctgaa tgataacttc ctgtattatc tgaaaatgaa aaagggcgaa
     8701 caagtttgtt ttccttcaaa ttttttatga caggattact tgaacgagaa aagcttgttt
     8761 gtggcaatcg agcttcaatc aaatagcgac tgctgatttc tttatcttgt agagaaataa
     8821 catagcggtt gtgcttttgc tgaatatcaa atggcggttc gatcaaatga aaaatacccg
     8881 catctatcag tgctgctaat tctttcgtcc gaatgattgg cggaccgatc gttaaaaagc
     8941 tatttagatg agtaaaccat ccccatagta tttctttgta ttccttagca gaaaaaacgt
     9001 gccattcaat agcctgccgg attggatcac gccagtcttt taatgcatca aatgttgaag
     9061 caaatgctcc tgtgcaattg cctttttccg cttcttttat ctgcccttca ataaatattc
     9121 ggctagcttc tgaaaagggc tcattctgat aggcaagagg agtttctaaa tacgaccaat
     9181 cccatctata agggattagt gctggatgct ttccgagaca ctcctctgaa gagagggaga
     9241 gaaaatcatg ctcaaaattt ttctttggaa tcggcaagtg atattcttcg atgattttct
     9301 tgtaataaaa caactcaacg tcttttttca gatagtcaaa aaatgtttcg ccagtcaaaa
     9361 atcccgactg atgccattgc tccaggtttt cttttgttag taaccgtggc catgccattg
     9421 ctccgccttg cttttgattt cttcctcgag ggtaataagg gagccccctt cttgatccac
     9481 tgtatacaat cggttccttg cctgatggat gatagaccag ttttcccttt tcgttctcga
     9541 agcgccctcc tcttgatact gtaaacaatc cgacataatc aaaaaaactt aagcctagac
     9601 ctcttaaaaa gacattttct ttaggcggta tcttcgaaag atctacatct gcaggattag
     9661 aaggaagctg atacagtaaa acatttttct ttgcgtagtc tacccacact ctttcttctt
     9721 ctgtctcatc attttcccag tgaccgcttg ctaaaacaag ctgatcgata gaatagcttt
     9781 gctgatctgt ctgtaaaaga taaccatctt ttcttttgct tacttttttt actaaagaat
     9841 aaacaaactg aatagaagct ggaaattctt cttgcaactt tgagaaaaac cagcgttgat
     9901 agagtccgta taaacatcga gtagattgct gatctttttc cagacgattg atttcttcta
     9961 taattagttg cttatttttg gttaccgtct gttcgatata atctgtagca ggtcctttac
    10021 tccattgaaa caagttgggt ccctgtgtta ccaatcctcc actggacaat gtttcatctg
    10081 taaaaagcgt cacttgttga gaaacagaat tcattaataa ctccggtggc tgattcaacc
    10141 gccaaactcg acctccagga ccattgggat cgaaaagaaa aagttcaaga gaatgtctgt
    10201 cctgattatt atggagaatc ctttctgcta cacttaatcc tctaggtccg gcaccaatga
    10261 tccctatttt catttgcatg cctccatttc gataaatttt ttcaataaaa cagcatggtt
    10321 ctgatgttca ttttttgcac cataaacaaa agtaatgact ggatgttctt tcataagttg
    10381 actgagttta aaaatagctt gcttcttttc ttcttcgaaa atcaattctt gctggtattt
    10441 ctttgcaaat gaagcaaatc gttctggtac atggtcaaaa gttttgcgtg cttcattact
    10501 aggtgcaatt tctttcaacc acagatctaa ctgcgctttt tcttttgaaa ttcctcgtgg
    10561 ccagaatcta tccacgagta ttctatagcc atcctctttt tcagcatcaa ggtaaacacg
    10621 ttttaatttg atcatagttg tttccttctt tctacctttt cgcttagctt atatatgtag
    10681 ataatagcac aaaataaaaa aaatgaacgg cacaactatc cattgcgccg ttcatgtgat
    10741 cttggtcaaa atctaatgat tgattcggtt tttaaagctg tcatacaatc atctgtttta
    10801 tactttttta cttttgattt ttccagacca tccgtcatat ccacccttaa gtaaataaat
    10861 ctcttgataa cctgctttac gtaatttatt tgctgtacgg atgctcaaac ttttcttttg
    10921 atcgtaaata tagacaggct ggtcttttcg aatagatcct aatgaatctt tcaaaacagt
    10981 gtaagggatg tttcttgcac ctaaaatatg cccagcatca aacacatctt tttcgcgaac
    11041 atcgattact tgtgcttttc tcattccttc tcgaaattct tcttctgtca aagtcgttgc
    11101 tgaacgtttg gccattactc gtagatagac ttcccaaagg atgattgcaa gcacaatcaa
    11161 tagcaaaatg atattaatta cccataaaac catgtaatgc ctccgttttt ttaagcaaat
    11221 ttcaaggcta atgaggcagc accaatgaca ccagcttcat taccaagctc tgctaatttg
    11281 atttttgttg attctcttac ttgcgggaaa gtaaattctt taaaatattt ctctacacgg
    11341 ctacgtaaaa attctcctgc tgccgatact cctccaccta acacgatact tgacggattt
    11401 aatgtattcc ccaagtttcc gcaagctaaa cctaagtaga agcatacgcg atcaacaacc
    11461 atcaatgcaa acggatcgtc ttcttgagcc atttcaaaga tatctttact tgttacttct
    11521 tctccgttgt ctagcattgc tttcagtttt gaatcccctg cgtattcttc tgccaagaaa
    11581 cgacctaggc gtaccacacc tgtagcgcta gatacagttt ctaaacatcc gcgtttaccg
    11641 caagtacatt cgaacccttc aggatccaca gtgatatgtc cgatttctcc tccagctccg
    11701 ctcacgccgt gaatcaaatt tccgccagca acgatacctc cgccaacacc tgttcctaga
    11761 gtaacgaata caacgtctgg atcgttttct ccagcacctt tccatcgttc acctaaagca
    11821 gcaacattcg cgtcattatc gatagcgaat ttgattcctg ttcctgcctc gatcagtttt
    11881 ttcacaaatt gaacttctgt ccagtttaag ttatacgcac caataaccgt tccagcttcg
    11941 ctgtccacgc ttcctggggt acccatgccg attccgataa agtcttcggc tttcatatta
    12001 tataaattca aacgatgatt gattgattca atgatttcag gaacgatatg cttaccatca
    12061 tccaaaatat tggtatcgat gctccatttt tgctgaatct ctccttctgg tgttaaaatt
    12121 gcaaattttg ctgttgttcc acctaaatca atcccaataa tcttcttgtc cattacaaat
    12181 ttcctttctt tcggctttct tcaattcggt gctctctttt taatatgcgc cacgctgtca
    12241 tgtatgtatc atgatcaatc aaacggctat catacagatt acgcaattcg atagtcatta
    12301 attcaatgtc atagatgcgt gcacccacat agataaagat gccaaatttt ttgaacagct
    12361 gctgtacatc atataagctc tccatcttta agcacttcct tcatattata caagtaaacc
    12421 atattttttt aaacccagta aaatacatat tacaaaaaga aatacaaaaa ctagtgtaga
    12481 aagaatcctt tggtgtatcg aatagcttgt ctgttgtttc ggtagaccca aaacattccc
    12541 taacactaat ccgccaatta gcccaccgac atgtccccaa acatctacag aggtatccag
    12601 aagaccaaaa acaaatgtca acacaataaa taaaagaaaa tgtctgacta gctgctggat
    12661 cgctgggtta tgtttaaaat gaaaccctaa gataaacaac gcaccgaaca aaccgaatat
    12721 cgaagtactt gcaccaccag acagaacatt cgcttcgtta aaagcaaagc ttgctgtatt
    12781 tcccattatc ccgctgaaca tataaatcaa gaaaaaacgc caatggccat agattgcctc
    12841 gatttgctgc cccataaaat acagtatcag tgagttcatt acgaaatgca tcagtccaaa
    12901 atggatgaag attggtgtca ctaaacgcca ccattcattg aagtgaacaa gataaggacc
    12961 atacattccg gcataataag ggatctcaag tcgcggaaat aattccatca tgaggaagac
    13021 cgctgtctgt atccctaaaa aagcataggt ccataacggt cgtcttaacc atgcacgcaa
    13081 ttttatttgc tgttgataat tcatgaaaac ctcccatttc attttagtca ataaacaatt
    13141 gttggatctt ctgatcaaag ctctccggtt gccattgttc attcaattgt tcacgaaaaa
    13201 caaggctaca tgttttttta tgaaaattat ttaaataccg gtcgtaataa ccaccgccaa
    13261 aaccgattcg ataaccggct ctattgtaga taatacctgg tacaatgagg agatcgatca
    13321 tctccggcaa atagagcttc tctgattgtg gctcaagaac accaaaactc gaacgtacaa
    13381 gaacggactg ttcatccatt tcataaaacg ccatctgacg tttcggcaaa gttttaggta
    13441 caactatttt cttttgttct ttttctgctc gttggataac cggctgtgta tccagttcaa
    13501 attcattcga taaagtcgtc cctatcactt ttgctgactt ccagtactcg ctggcgaata
    13561 gctgtttata gattcgctgt tctttttcct ttttcttgcc acttttggcc agatttttca
    13621 gcttttcgat tcctttccgg cgtaatctta ctttcaattg gtccaaaaac tcacccttcc
    13681 ttctctcata aaatggtata ggaaaaaaag aaaaatagca atcgaacttt caaataaatg
    13741 cataaataga ataaaaagaa gacctagaaa tgattggcct tcctttttta atgaatttca
    13801 actgttcagc gcgtgttttt gtctgataaa aagtgcatac agaaaatatg gcgcaccaaa
    13861 aagggcaatc aaaattcctg taggtacttc cccacttggt agaatcactc tagcaaccat
    13921 atctgcacta agcagaaaaa tggaaccgaa caaaccgctc agaacaaagg tccagttatt
    13981 ttctttacgt acgactagct tcgcagcatg aggagctagt agtcctacaa aagataaact
    14041 acctgtcacc gctacactac cgcttgccaa agcaacagct aacaacaaaa ggattttttg
    14101 acttttagcc acgttaaggc ctaatccttg cgcgcgttct tgaccaaatg atagcaatac
    14161 aatcctctgt gcataaagtc ctcctagcgg aattaagata accgcccatg gaagcaatgc
    14221 ccaaacataa ggccagctcg caccccagat tgatcccgct aaccatgcat tcacaaatcc
    14281 ataattttcc ttagataatt ggatcgtgca tagcaatgtt aatgcagaaa ggccggcatt
    14341 aacggcaata ccggacaata agattcgatt catacccaaa aagccatttc gatcataggc
    14401 cgcaagatag acacagcaag cagctaaaac accacctaga aaggctacaa ctagcaatag
    14461 aaacggcagc gaatagtgcg aaaagaaacc taaataaagc atcactaaaa atcctgctcc
    14521 ggcattaatg cccaatatac tagaatctgc caattcattt cttgttactc cttgcaagat
    14581 ataaccagaa acgccaagac aagctcctgc taataaactg atcagtaatc gaggaactcg
    14641 aaattctcta acaatcagcg ccaaagaaga tgattctttc cctatgaacg tggcaaaaag
    14701 ctgatccagg taaataaaag gcgtaccaat cattaaacta cctatagaca ggatgacaag
    14761 tacaaaaagc aagaaataga acgtccaata tatttttttc accgttgttc cctccttact
    14821 tgtatcagta aaaacggaac gccaatcaat gcaatgacta atccaaaagg tgtttcaaaa
    14881 gggggattga tcatcctcgc tgtaaaatcc gccaacataa ccaatccacc gccaactaaa
    14941 aaagagacag gtagaacata acgatagtcc gttccaacca ttccgcgaac aactggcgga
    15001 accaacagcc caataaatga aacaggtccg actaacgaaa cggcaatggc tgctaacact
    15061 gcaactagac agaaaacaat caaccgcgtt cgtttaggat gacttccaag agctaatgca
    15121 tgtgtttcac tcaaatgaat caaagatatt gcttttccta atgaaaaaga aaccaaaaca
    15181 gcaagtatat ataaaggaaa caatactgct aattgtttcc atgtgatatt ggcagctccg
    15241 cctacaaacc aaaaagcaag atcctgctgc agattaaaga attgggtaag tgcttggctg
    15301 atagcagtaa aaaaagaact aatagctacg ccagctaaaa tcagctgtaa aggattcatg
    15361 cccattttcg aaaactgcaa aaaaccaaat acgagcaaag ttacagcaaa agaacctgcc
    15421 aatgacatta gaaacacaga aaaatcagaa gctttaggcc aaaaagcaaa actaagggcc
    15481 attgccaacg aagcacccgc gttgattcct agtaagcctg aatctgctaa aggatttctt
    15541 gtcactcctt gcatcaatgc accgctcaat gcaaaacaac tccctacaag aaatgctccc
    15601 gccacacgag gcaaacgaat atttcgtatg atttggtgac tttgttttga tctatcaaaa
    15661 tcaaacaacg cctgccaaaa atctgctttt tccacagtcg ctgcgccatt tgaaagagca
    15721 acaaaaaaaa taatcaaaag aacaaaacag accaaaagca ggagtctatt tttcttcata
    15781 gtcctcataa ttgatcctcc tgctcttttg gcaacaaatc ataggttaga aaaactggtt
    15841 tatttgttct tggaagcgtg acaatcgttg catcaatctc aaatattttt ttcatacact
    15901 cttttgtgat cacttcttca ggtgtacctt ctgtgatcaa cttaccgttt ttcattgcta
    15961 ataaataatc tgaaaaacgt gaagcatgat taatgtcatg aatcgacatc aagatcgttg
    16021 tttgtttcat ttgattgatt tcagtcaata actgtaggat ctctaattgg tgtgccggat
    16081 ctaaaaatgt tgtcggttca tccaagatca agatatctgt ttcctgagca agcgccatag
    16141 cgatccagac acgctgggtt tgtccaccag acagcgttgc gatatcccgc atacgaaatc
    16201 cagctagacc tgtagcatcc aacgcccact caattgcttc atgatcttgc gtgtccatgc
    16261 cactgaacag tttttgataa gggtatcgtc cgtaagatac tacttcttct actgtcatac
    16321 ctgtagggtg ttcactagtt tgtgctaata atgcaatttt ttttgcaagt tcttttgaac
    16381 tgtatgtaga gatatcagag ttattgacaa tgatctttcc tgatttcaca ggtagtatac
    16441 gggtaaaact tttcaataaa gtagattttc cacttccatt tggcccaatc aaactagtga
    16501 ttttcccttc aggaatcgta acagacatgt tttcaatgat ttttttgttt tcgtagccgg
    16561 ctgcaatgtc agctgctctt aatctcgtca tttcacccat cctttcgctg aaaatcattc
    16621 tcaattatga cgctgtttca acatttcgtc aagcgctact ttcatatcta tagccaaatg
    16681 aagcggttga cagcgttcga aatatcagga ataataacca atgagaaaga ttctcaatta
    16741 tgttaggagg aaagaaatga aattaaaaac aaaagtattc acattatcta gcatattgct
    16801 gctggctgga gtactgagcg cttgctcccc ctcttcagat aaagaaacag ctacaactgc
    16861 caattcgacg acttcatcca atagtactca tacagtcaca gatacacttg gacataaagt
    16921 agagattcca aacgcgccca aacggatcat cggcagttac ttagaagact atctgattgc
    16981 actaggggaa aaacctatcg cccagtggac tgttggcagt ggttccattc aagattatct
    17041 gcaagatgat ttaaaggatg tcccaacgat ttcttatgac cttccttatg agaaagtgtt
    17101 aagctttgag cctgatttgc tgttgatttc ttcttccgct actgtcgaag gcgggaaata
    17161 cgaacaatat agcaaaatcg cgccaactta tgttgtaaaa aacggaacag atgtcacatg
    17221 ggaagaacaa ctaaaagatg tcggcaaagc tttggcaaaa gaaaaagagg cagaaaaaat
    17281 catttccgat tatcaaaaat ccatcaagaa aactcgagaa gaactagcag ataaaatcga
    17341 gaacaagaca gctgcagtgt tgtgggtgac caacaactca gcctttatgg tcagcaaaaa
    17401 tcggtcaagt ggaagaatcg tatacgacga tctggaattt ggtgttcctg atctagtgga
    17461 agaggtctcc aaaaaagcca cttctgactg gtctgctgtt tcctctgaaa agctagctga
    17521 attagatgct gactacatca tccttgtgaa cagtgacaaa gatgctgcca tgttcaatga
    17581 accaagctgg aagaatctaa aagcagtgac agacaatcat gtactgcaat tcggacctga
    17641 atctagttgg ctatataacg gacctattgc cagtcaggaa atggtcaatg atatcaaaga
    17701 aaaactaaat tgattttaac taaaaaaagc atcttcacga ttagtcgtga gatgcttttt
    17761 ttcttattaa caaactagac gcatagattt tattcttctt ttgaagcaac cctatttttt
    17821 actctttccc atttttgaaa acgatgggca tatagatttt cttgatccgt tatcccctca
    17881 tactcctcta taagggcgaa gtctgaaaaa tcaatattcc caatatacgt atcgccttca
    17941 aacgtgctat cgatcaatgt tcgccagatc cgaccagtgt aaggaagaag ttcttgaaaa
    18001 atccgactac cacccgaaac atagatatct tctgagatcg tttgactata cgacaaaagt
    18061 tcatcgatcg aatgcattac ttccgcattt tctttcgtgc gaaaatttgg ttgcgttgtc
    18121 aacacgatga tatggcgata aggaagtgaa agatccccca ttccttcata ggtctttctt
    18181 cccatcacta aaattcgatc cattgtatgt tcgcgaaaga aacgcatatc atttggcaat
    18241 cgccatggta acaaaccatt tttgccgatc agaccatttt tatcttgtgc ccacatggaa
    18301 ataaacatat tatccgctcc ttatgatttt agatgttttg agataaaaaa agtctcaatt
    18361 ctttgaccag caaggaattg agaactttaa aaacattatt ttgtttcacg gtgcaaagta
    18421 actttgcgtt cgcgtgggca atatttttgt ttttccaaac gatcaggatt gttacgtttg
    18481 tttttgcttg ttagatagtt acgttcttta caagaagtac actctaaagt gatgtttacg
    18541 cgcatgtttt tccctcccat ttttacaaga atcctctaag atgactctca gacttgatta
    18601 tcttaccatg attttttcta gattgctagt gtttttcacg attttgttaa gtaaaactac
    18661 ttgacgataa atacaacggt tttacttctc aaacaaaatc tttttctttt tgtataaata
    18721 cccttacact tgtttatata gattatgttt tttaataaag ggggatacta atatgccatg
    18781 gaacatgaag gactttcctg catcaatgaa aaacttagat aaattgacaa gaaaaaaagc
    18841 aatcgacatc gcaaatgcat tattagatga agggtaccct gataaccgtg cgatcccaat
    18901 tgccattgat caagcaaaaa aatggtatga agatgctagt gaatcagaaa aacgaacttt
    18961 cgaaaaagaa aaaaatccaa gcaagacgga tgaacacgat acgaatcctc gtgctggaaa
    19021 actgcttgat tcagatgtga tcgtagaata tgaggaagaa caatggatcg tgaaatctaa
    19081 aggggcaaaa aaggcaagca accattttga tacaaaaaaa gaagcaatcg aaaaaggaaa
    19141 gcaagtagca caaaacaaag aatcaacttt gttcatttat aaaaaagatg ggacaaaaga
    19201 aaaagaaatc gattattgaa catatgtaaa ggggttgtga cgaaaatcgt gtcacaaccc
    19261 ccttatgttc gaaacaatgg tgttcctctg ccaactccga accataagtc ataatatcta
    19321 taaaacggga agagctgttt tttgaaattg tttcttatca ctcagtgtta aacggtagga
    19381 gaaaacctct tttttattga tatttgattt tcatcaaaca gtcacatagt cgattgcttc
    19441 tgcttgcgga atcgtttcat ctacaaagta gactttatac catgtcaaga aactgaagat
    19501 tgtccagatt tttctttgct cttctttacg tccttcgtga tggtcatcta gcaacttcat
    19561 gattttttct tgatcaaaaa attcttttgc aaaatctgct tcgaataatg ctttgacttg
    19621 ttcgtagccg tgttcttctt tcaaccaagc tttgattggc acagggaatc ctaatttcac
    19681 acgattcgac cattcttccg gaagatgttt gttcgcagct tgtctgaaaa tatcttttgt
    19741 gttgttttga ttcaataaaa acttacttgg tatttgttga gccaatttca tgacttcgat
    19801 atccaataaa ggtactcgga cttccaaaga acttgccata gaaagtttat ctgcctttaa
    19861 caagatatct tttggcatcc attgatgaag atcgacatat tgcatcttat tgacttcatc
    19921 tttgattcct tctgttttct cgtaatgaac agtcatgatt tcatccacag atggtgctgt
    19981 ttgatatttt ggtgttaaca cttctgatgc ttctgattct tcgaacactc gagcatgacc
    20041 gataaaatat tcttttgcag gagcaagtga ttcatataaa tggatccgtc catggaagtt
    20101 tttcattttt ccgattttac gtcctaaatt atagcgtgtt ccttttggta attttttcaa
    20161 tccttgagca atcacacgga tgaattttga attcgtatgg aatccatacg tttggtatcc
    20221 cgcaaacagt tcatccgccc cttctccaga ttggactacc cgcacacttt gtgaagcaag
    20281 atttgctaaa aagtacaaag gtacgcaaga aggatttgaa tcaggttcat ctaagtgata
    20341 ctggatcaaa gggaatgctt tgaacgacat gtctccgtcg atcactgctg ctgtattatc
    20401 taagtccaat agttcggtta gttttctggc ttcaatcgct tcattatagg ttttatcatc
    20461 aaacccgatt gagaaagaat gatctggttt caatacagag gttacataac tggaatcaac
    20521 cccgctggat aagaaagaac caacttctac atcactgacc gtatgtgctt caatagaagc
    20581 ttgaacggtt tcgtcgattt tttcgatcca ttcttgtcgg gaatgcgttt cttttgtttc
    20641 aaaatctgca tcccaatatt gctgaatgtc tatttttcca tctttatacg tataataatg
    20701 gccttcaggc aaacgataga catctttgaa gaatgtttcg ccgtttaatg gagaatattg
    20761 gaaagtcata taaggtttca acgcttcttt gttcaattct ttgttaaaat caggatgagg
    20821 cagaaaactt ttgatttctg aaccaaacat aaatgttccg ttcatttctg cataataata
    20881 tggtttgatc ccaaagtggt ctcttgcacc gaataactca ttcttttcat tatcccaaat
    20941 agcaaaagca aacattccgc ggatcttttg caataattct gttccccatt cttcgtaacc
    21001 atgcaaaagg acttctgtat ctgcatgtgt ttgaaaaacg tggccagctg caatcaattc
    21061 ttctctcaaa ggctggtaat tatagatttc gccattaaaa ataatgatct ttgtccgatc
    21121 ttcattgtaa ataggctgag taccgccctc taagtcaata atacttaatc ttctgaatcc
    21181 taaagcaaca tgtttatcga tatattctcc tgaactatta ggtccacggt gaacgattcg
    21241 atccatcatt gtattaataa ttgttttttt gttgtccttg tcgttaacaa aacctacaat
    21301 accgcacatt taactttcac atcctaatat aaaatta
//
LOCUS       DAZULG010000045        21007 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000045 DAZULG010000000
VERSION     DAZULG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.046"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..564)
                     /locus_tag="ACG9CY_001457"
     CDS             complement(76..564)
                     /locus_tag="ACG9CY_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS4937973.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(765..1031)
                     /locus_tag="ACG9CY_001458"
     CDS             complement(765..1031)
                     /locus_tag="ACG9CY_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4937974.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(1031..1294)
                     /locus_tag="ACG9CY_001459"
     CDS             complement(1031..1294)
                     /locus_tag="ACG9CY_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4937975.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            1815..1955
                     /locus_tag="ACG9CY_001460"
                     /pseudo
     CDS             1815..1955
                     /locus_tag="ACG9CY_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(2097..3920)
                     /locus_tag="ACG9CY_001461"
     CDS             complement(2097..3920)
                     /locus_tag="ACG9CY_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4937976.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(4518..7223)
                     /locus_tag="ACG9CY_001462"
     CDS             complement(4518..7223)
                     /locus_tag="ACG9CY_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS4937977.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(7529..8476)
                     /locus_tag="ACG9CY_001463"
     CDS             complement(7529..8476)
                     /locus_tag="ACG9CY_001463"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS4937978.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            8666..8935
                     /gene="rpsN"
                     /locus_tag="ACG9CY_001464"
     CDS             8666..8935
                     /gene="rpsN"
                     /locus_tag="ACG9CY_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4937979.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(9111..9473)
                     /locus_tag="ACG9CY_001465"
     CDS             complement(9111..9473)
                     /locus_tag="ACG9CY_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4937980.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(9484..10605)
                     /gene="alr"
                     /locus_tag="ACG9CY_001466"
     CDS             complement(9484..10605)
                     /gene="alr"
                     /locus_tag="ACG9CY_001466"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS4937981.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(10621..10971)
                     /gene="acpS"
                     /locus_tag="ACG9CY_001467"
     CDS             complement(10621..10971)
                     /gene="acpS"
                     /locus_tag="ACG9CY_001467"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS4937982.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(11101..12612)
                     /locus_tag="ACG9CY_001468"
     CDS             complement(11101..12612)
                     /locus_tag="ACG9CY_001468"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4937983.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(12934..14286)
                     /gene="murF"
                     /locus_tag="ACG9CY_001469"
     CDS             complement(12934..14286)
                     /gene="murF"
                     /locus_tag="ACG9CY_001469"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS4937984.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(14439..15515)
                     /locus_tag="ACG9CY_001470"
     CDS             complement(14439..15515)
                     /locus_tag="ACG9CY_001470"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS4937985.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            15711..17456
                     /locus_tag="ACG9CY_001471"
     CDS             15711..17456
                     /locus_tag="ACG9CY_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS4937986.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(17736..18173)
                     /locus_tag="ACG9CY_001472"
     CDS             complement(17736..18173)
                     /locus_tag="ACG9CY_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS4937987.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(18157..19530)
                     /locus_tag="ACG9CY_001473"
     CDS             complement(18157..19530)
                     /locus_tag="ACG9CY_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937988.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(19852..20340)
                     /locus_tag="ACG9CY_001474"
     CDS             complement(19852..20340)
                     /locus_tag="ACG9CY_001474"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4937989.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(20362..20964)
                     /locus_tag="ACG9CY_001475"
     CDS             complement(20362..20964)
                     /locus_tag="ACG9CY_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4937990.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6041 a         4486 c         3808 g         6672 t
ORIGIN      
        1 tggctaaaaa catatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa
       61 accggtacac gattcttatg gactgcatcc gtcgcaacta gacttggcga tacgatgatc
      121 cttcaccaga agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac
      181 attggcgtaa aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc
      241 cgaaagcgcc acgactttaa cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc
      301 tttggatacc cgctctgcta aaggaacgac actggtattg tatttcgtat ctgtatcttt
      361 tccaaaagac catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc
      421 cattgctttt ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt
      481 cgcctcactt tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc
      541 aattttactg attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca
      601 ttccattcct aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag
      661 aaatcatgca ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg
      721 ggagactctt tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc
      781 caggcagaat aaatttctac aactttattt tcctcatcaa ctttgtaaac cactcgatgc
      841 tgtctattca gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac
      901 tgcgtgggca tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt
      961 aaattcgttt gcttgatttt tcttaaatcc actttcgcag agtttttgat tgcgactgta
     1021 tagttactca tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt
     1081 ctttttcgcg gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt
     1141 gaatcgctct ccagtcttcc agcccaatca atacagcatt gtttccacta cgatcactaa
     1201 taatctcgat ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac
     1261 gagcagtgct aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa
     1321 ttatagcgta cttgattaag tacgtcaaaa aaatacaata aagcacttaa attcagcaaa
     1381 cttttcctct tttttacata gagaaaaaga ccccttttta gggaggatct ttgtccacgc
     1441 tcacttctta ctcctttacg actagcggta gccaaacgta aagtaattgg taacaacgta
     1501 tcatctttag caaaggtcga atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg
     1561 aaggttcctc gttgcagtgt gacttgattg atatcttgaa taacctgagg atacgcacga
     1621 aagatttgta acggttattc aaaactgatt ttggccgtca agtcttctac tgcctcaaaa
     1681 atgattgcct ctttcgtctc ctgtcattca tgttcactat cgatttgata cttgcttaga
     1741 ccgttgggtt tgtgaatcac caattaaaaa tggcactgag ttatggtaac aaaaaatggt
     1801 cacttgacga ggctgagatt cctacttatg aagacacatg gcacttaaga gtcgaggtaa
     1861 aggaagaagc tgaaaatata ggaggctgta cattatttgg caaaggattc ggaaattatg
     1921 atcaagacat caaaatcaaa atgttttaca gctaaatagg aagaataata acatttcata
     1981 cgctgttccc atctttaatg gcttatagag ggcaccttta gctgagaata agacaaaaca
     2041 gaccctctag cttcaattaa gagctagagg gtctgttttt gattttaaaa cttggtttat
     2101 ttcttcaaat ggtagctata ggtgctgaca acgatatttt cacgccaaga caatcgcaat
     2161 cttaaccgca aacttgtttg gttatggaga atattctgcc agcgtctgtt tggtacaaat
     2221 tgaggaatca acacagtagt tgtatagttt tgtttcgctg catttttact tacgatatct
     2281 acataacgga taattggatt ttcaatcgag cgataagaag agtggacgat tgaaaaacgg
     2341 acatctggga aatgcttttt aaattctgct tcgatttctt tttctttttc gacatcttcg
     2401 tctagcgaaa catgcatcgc aacgacataa tcaccgatcg aacgtgcgta attcaacgca
     2461 ccgatattta cccgtgtcac attcccgact aatacaagaa cggtattccc gtcataatca
     2521 tgtagttttg catcattttc taaacgaagc tgttctgcta cttttttata atgatcatgg
     2581 atcttataaa aaatgaacat aacgattggc ataatgatga agaaaggcca aatgtcccct
     2641 aaccgataga caaacaaaat cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg
     2701 gcaatcgatt tgcttaacca tctttttcct tcttttttcc atttgacgac catccctgtt
     2761 tgagataaag caaatggaat gaacacgccg atagagtata acggaatcaa gcgttctgtt
     2821 gagccatgga aaataaacag caatacgatc gaacctagcg ctagtgtgat aatcccattg
     2881 gaatagccta gtcgatcccc acgatcttga tacatgtgag gcatgaattt atcttttgcc
     2941 agattgtatg caagaactgg aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc
     3001 agcgctgtag caaattgaag gacgtaatac aaaataccat gtccaaagac agctttacca
     3061 atttgagaca aaacagtgac ttctttttca ggaacgatcc cataccaata gttgataaat
     3121 gtgatcccca cgaagaagaa acctaagatc aatgccatca tcgtcaatgt agcagcggca
     3181 ttttttgctc ttggcttctt aaagaatggt acagcattac taatcgcctc caccccagtt
     3241 aaagaggaag aaccagaaga aaatgctcgt aaaatcaaag cgatagaaac acccggcaca
     3301 gccgctcctg gaagtgcagt agcttgcaat ggcacagcac ctgtcacgat attatataaa
     3361 ccaacaacga tcagtaacga gatcacaagg ataaatgtgt acaccggaaa taataggaac
     3421 gatgcagatt ccctcagccc acgaagattc agcatcatca gtagcaaaac gattgtcaca
     3481 gaaatagcga cttgatgacc gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct
     3541 gctgagacag atactgctac tgtcaacatg tagtcgatca ataaagagcc accggaaatc
     3601 aagccagcat tctttccaag attctcactg ctgacgacat atgctccccc gccatgagga
     3661 taagcatgga tgatttgacg atatgatagc gttaatgaaa tcaataaaat aataacaaaa
     3721 gcggcaatgg gtagcgaata ccagatggct gcagcagaca aagcgactaa aacgacaacg
     3781 atttgttccg taccatatgc aatagatgat aacgcatcag acgacaataa tgccaaagca
     3841 gcaaatcggg ttaatttgtg ctcatcattt tcagcagatt ttaaaggctt acctactaac
     3901 aaccttttta agtaatccaa tttctaaact cctttacaca caattttact tcaagtttta
     3961 tagtcgaaaa tttatagtaa acaagatgtt atagtacgct cactgacctt aaaagtcaat
     4021 aaactttaag aaaataattg gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt
     4081 taatacagca aaaatctaac atttttttat gggctttttc tttgaattct ataataaaag
     4141 gatagaactt gacttagaaa atcaagttct atcctttttg atatcttaac ttttggaaga
     4201 gagatgtaag gctgctttct acccatcatt cttactactc gaagcatagc gcagttgtca
     4261 caacctcttg ataaacggtg tttaaaagct tgcatctgcg gtaataagcc caaccaaatt
     4321 aaaaacataa gaagctgttt ttatttggtc gttcttatta cttgatgcag agcagctttt
     4381 ttacaacctc ttggtacacg gtgttcaatc agctgaccct cggtattagc tcaaaaaaac
     4441 ggcaaaatat gaggaactat ttttccattt ttttctctaa tactcaggtc aaaacactga
     4501 tttcacaacc tcttttttta atgaaaaata tggctctcat gcgccgcttc tttgaatttc
     4561 gtcatttttt cttcgatgaa cggatagatt gccgttccta cgattccaaa gacaacaaac
     4621 aaaccgatca tgatccagat tgctttcgta gcatttggcc aataaattcc tcctgcagat
     4681 tcccgtaaca agtttaccgc atgagtaaat ggcaagaacg gattgatcat ttggaagaat
     4741 ttccctgaga cttggatcgg atagttcccg cctccgccag agatcgacag tactaagatg
     4801 ataatagcga tccctttacc gacattatcg aataatgcag ccaagacata gacgatcatc
     4861 atgaacgcta aggcgatcaa gacagcaaat gacacgctgt agaccggatt ttggacatct
     4921 actcctagta agaaaatatt ccctaatgta acgatcaagc tttgcgcaat cgccattgtc
     4981 aaaaatgtca gcatacgagc aacgaattgt tctcgtttcg taaaattgtt tcggtctttc
     5041 tttcctaaat agtattctgt cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga
     5101 catagtgcag tataaaacgg ggtgctagct gaaccgttat ttgcgattgg ataaagcgca
     5161 ttggtttgta gctcaacagg ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc
     5221 aataatttga tgacttcgcc tagatcgact tcttgttcgc cttttcggat cgcgtctgct
     5281 gctttttgga taccagcttt gatatttggc cagtcattgt tgatcaagtc cgcagcagcg
     5341 ttcacagctg tttcgacttc tggcattttt tcatttactg ttgtcaatgt accggtgatt
     5401 tcatttttta gtgctggcca atcattttgg ataaaatcag cggccaatcc taatttttgc
     5461 tgaatgatcg gtaattcatt gttgtatagg tctgcccctt cattgatgcc attgacgatg
     5521 gtatccatat ttccattcag cattgtattg gcatcatgga tttcttgtcc aattgcaggc
     5581 atttcttttt gatatttttc taaaattgcc actgcattcg ttacagttgc tttcgttgag
     5641 ctgagaagcg aatcaaagtc gatctggtcc gctttgctca gtgcgccttg tgcagtccca
     5701 atcgtatcga tgacttggtc tagtagagaa gtgacagtgg aactgatttg acctgtattg
     5761 ataccagcaa tgatgccatt gatattatta acaacggcat caatttgatc tagcaattca
     5821 tcaatagcct tcgtgtcttc attttgaaca gctgtgttca aagcagatag gcggttttgc
     5881 agattgctta gcaagggttt taattgattt agctgatcaa taattggctg taatttcgta
     5941 tttccagcac ttgtttgcaa tttagtcagg aactcgacca gtttatcgat tgcattttgt
     6001 tgttgagata ttccgtcaat aaagccgttc aatacattgc tgatgttttt cttttcatca
     6061 tcagtcagct taccatcttc aatagctgtt cttacagtat ctattaacga acctgtcgtt
     6121 gttgccactt gtccaattga cttcaatgta acatctacac tgtttgtgat gcttggtatc
     6181 gcatctttca gctgagaggc acctgatttt gtttggtttg ctagatcact tgcctgattt
     6241 tctaaatttt tcacatctgg caagatatct tgtacttgtt ggatgatcgt caagccttgt
     6301 tttgcttcat cgattccttg gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc
     6361 tctgctagtt gcttaccggc attttggatc tctgggattt tttcttgcaa atcaaggatg
     6421 atttttgctt gatctttcaa ctcaggcatt ttgtcattca atgcaacgac tttttcaccc
     6481 atctcatcta ctttagggat gtaatccacg aattcatttg ctttattcag tttttccttg
     6541 atttcaggta gttgactttg taattcgagg acttgttttg tgtagccgtc gatcgtgtcc
     6601 aaattttcat ctgttgataa aatcatatct ttaactttat tgatacttac taggttcgta
     6661 tcgatgtcgt aacctatctc attgaatact ttcaataaag tcgaactggc tgttttgata
     6721 aattcatttg tgatttgcga ttggatcgaa cttgctcctt tatcggtgat tttcggtgca
     6781 atcgcattga ttttttcatt taccgtatat tcgatttttg gtttcttgat atcaccacta
     6841 gtaaaactca gcagatcttc tgagaaatcc tttggcaagt aaatacctgc gtaatacttt
     6901 ccggaacgga ctccgtcttc taattgttct tttgaatcaa cgaattgcca tcctaattgt
     6961 ttatttttat gaagcgattc gatgacttgc tccccaatgg ccacttcttt accttgaaac
     7021 tctgctggct tgtctgcact gtaaacagca attggcagct ctccagtatt tccatatgga
     7081 tcccataatg ctttgatgtt aaaccacgca tatagcgaag ggatgatcat gatcgcgatg
     7141 atcagaagtg ttgcaatcgg gtttttgaaa atccgtttcc agtcaagcaa atataatcga
     7201 agcgtatttt tgattgactg catagttctc ccccttcttc ttttttcagc tgaattactt
     7261 taccatgtat ttcatacttt tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca
     7321 tatcacggca attgtctcat tgtaatctct tacatattga gaaataatta aggattacag
     7381 cctttacttt atcatgaaat aaaagggaaa gataatttta tgccaaagtt tttatctatt
     7441 atacgtatac gcaaaaaagc taaaacattt tttgtctttg cccctacaaa aatgtttcag
     7501 cttttttcgt tttttattcc tgtatttctt atttttcgaa tgacaggatg attttcccag
     7561 caggaggatg tccttcaact agctgatgtg cttggatgac atgcttaagt gaagcagaca
     7621 gattcattga aataaaaaca tgaaaggctc ctttttggta ggctttgatt atgccagcaa
     7681 aggcttctgc atctagatat tcctttcttg gaacaaacga ttcataaaag ccttcctttt
     7741 tttgccgcaa atctagatct gggaggtcat ttaatgcaac atacctgccg cctggtttca
     7801 tgatctggat gccggtttct cctttgatac ttcctttcgt cgcatcgatt acaaggtctg
     7861 cctgatcagc aaattgcaga cctggatttg ttttatcata agcagcaaat gctgaagctc
     7921 ctaatttttt taccttttcc tcatttttac tggatgcact ggttaagatt cggattccct
     7981 tttcatggag aagctggatc agacttgacc cgaccgcacc tgatgctcct agtatcatta
     8041 cagtatctgt cggctggatt tccgtcaaat ggttgattag attataagcc gtaattcctg
     8101 gcgtgaccat tccggctgct tcttcccaac tcattttatc tggaattttt gctacttttg
     8161 ctgatggcaa cacgacttct tctccgtaag tcccgccgac tgcatggaca gctactcgat
     8221 cacctacatg aacagttgtg acatctgaac caatctctgt cacaatacca gcgccatcat
     8281 tacctggtac ataagggaat ttcaatgttc gtatctcttt catttctcct aaacgcaagg
     8341 caacatcata aggattcaca ctgaatgctt ttatttctac acgaacatgt ccagaaccga
     8401 cttctctggg atgagcatcg atttcttcaa aaacatcttg tgcttctccg taaccgttaa
     8461 ttgcaaatct tcgcatggtc accatctccc tttttcttta agtttacgtt tatgccttta
     8521 ttttgacaaa aaaaatgtct tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt
     8581 ttttcgcgta acgtgttaaa ttatttatct aaaagcgtaa agattacgat ttacgctaat
     8641 aaaaaataat tggaggaatt tgttgatggc taaaaaatca aagattgcca aagcaaaaaa
     8701 acagcaagaa acgattgaac gctatgctgc tttacgccag gaattaaaag cgcaaaaaga
     8761 ttacgaaggc ttgcgtaaac ttcctttaga tgctcgtcca gaacgattga aaaatcgtga
     8821 tttaatcgat ggacgccctc gcggatacat gcgtaaattc gggatgtccc gtataaaatt
     8881 ccgtgaatta gcacataaag gtttgattcc aggagtaaaa aaagccagct ggtaatccaa
     8941 gtctggctgt aatgtaaagg aggagggcgc atggaccact ccttggatca tcaaaaaaga
     9001 tacgttcgct tcttctagca gaaatgctgg tactagaagc agacgtatct ttttttacgc
     9061 acagctggtc cttttttata tgtaaaggct gcatggttac ttcgctatat ttatttagta
     9121 acaggagtca gtgcttcgat acccacacta attttcaatg ctcggtcaac ttctgccata
     9181 atgggtcgat ccaaatggca gaccttttct ttcaatcttg atttatcgat cgttctgatc
     9241 tgctccaaca ggataacgga gtctctttct attcctgttc cagtagcttt gatcccaatg
     9301 tgagtcggaa gcttaggttt agccattttt gccgttatgg ccgcaacaat gatcgtcggg
     9361 ctaaaatggt tacctagatt attttgaata accaaaactg ggcgtgttcc gccttgctcc
     9421 gatcccacga ctggagaaag atcagcaaaa taaatatctc ctctttttac cataggatcc
     9481 tccttattct tgatattctc ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg
     9541 gatcgtacct aattgatctg ctatatcctg catagtgatt tctttgtctg catttttgcc
     9601 gataagcgtc acttttgttc cgacaggaaa ttcttgaggc aatcggatca tcagttgatc
     9661 catacataca cgcccaatcg tttcacagta gttcccttct accaatagag aaaaaccctg
     9721 catctttcgt ggccagccat ctgcataacc gatcggtatc gttccgatcc actctgcttc
     9781 aggagtgata tatgtttctc catagccgat tccttcacct gctgggagtt ttttgacttg
     9841 aatcaattca gaaaccagct caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg
     9901 cccagaggga ttaagtccgt acattgctac tccataacga atcatattgc cgattgtttc
     9961 atcatgccat aaagccgttg cactattgct gacatgtaca tatcgaggaa gttcgggaag
    10021 ttttttcagc acttctataa agcgttcttt ttgcagattc caatatgtat catctgcctg
    10081 atctgctgta gaaaaatggg taaataaacc ttcccattct aatacacgac tttctttgat
    10141 gaatgcagct gcctctttta cttcttcagg tgtacaaaat ccgatacgac ccatcccagt
    10201 gtctgctttt agatgaatcg aaagaggcgt ctctagctta cttttctcta aaacagcagc
    10261 tgctgcttgc aaccattccc gtgtacctgc agttactgat aaatcatgag caacagctac
    10321 agtcagtgcg tcgtaaggaa ccatattcaa gataaggatg ggctgagtaa atccagcttc
    10381 tctcaactcg actccttcat ctaaatttga aacacaaaag cccgaagctc cacctgcaac
    10441 tgctgcctca gctgtttcaa tagctccatg accgtatccg ttcgctttta ctactgcaaa
    10501 caattctttt ccttgcggaa gtcttgcaac ttcatttgct acattttcag ttatagcttt
    10561 tttatgtatc actgctttcg ttgggcggtg ccaagcgaca accatcgttt ttccttcttt
    10621 ctagtcttcc aaaataattt gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca
    10681 tacattgcct tcatgaggtg aacgcgtcac aacaggctga ccattttcat tttttaaaat
    10741 ctcgacatct tgaaaggtaa cttttccgat cccagttccc caagctttag agaaagcctc
    10801 tttgcaagcg taacgcccgc ctaaaaattc tacttggcga tgaaaaggca aggactggaa
    10861 tagtttcatt tcatcagagg tcaagattcg tgcaatgaat tttggttttt cttcgatcag
    10921 ccgagtgatc ctaggaagtt cgactgcatc gatcccaatt ccttttatca tatgcttttc
    10981 tcttttcaat aaatttagaa cactctcatt ctaccaaatt ataaccattt tgccacaaat
    11041 aagtccacaa ataacaaaaa tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt
    11101 ttaatcgtta tttgaacgga ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc
    11161 atctttttgg ctgttattat ttttgctata accgcctttt gatttgtttc cacgataatt
    11221 accgccgtca cgacgattac ggttgtttcc gccaccacgt ttgccatttt tattgtatcc
    11281 ttttttctgc attggcaatg ggcgttctgg tgtgatcttc actggcactg catcagctgg
    11341 atcttttgct gtcgttttta gcaaaagtgc aactaaatct tgtgcagaat attcttctaa
    11401 taatttatct gctgtttgca ggtatttatc tagaccattt tctgcaagtt ttgtttcgat
    11461 ttgttcaact gcagcaccta attgaccttt gaatgcttct ttttcggttg gtggacgtaa
    11521 agtcgtcatc cgttttttcg tcaagttttc aatcacatgc aagtagtcca tttcatttgg
    11581 tgtaacgaaa gtcacagaca taccgccttt tccggcacgg cctgtacgtc cgatacggtg
    11641 aacgtagctt tctggatctt gagggatatc atagttgtac acatgagtga cacctgaaat
    11701 atctaatcca cgtgcagcaa cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa
    11761 tgaacgcaat acgctcattc gtttttgttg agacaaatcg ccatggattc cttctgcacg
    11821 gtaaccgcgt gcttctaatc cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa
    11881 gacgattgtt aattcaggtg tttgaacatc aaacaagcga gtcatgatat cgaatttttc
    11941 gtattctttt gcgcggacat agtattgatc gatcaagtca gctgtcattt ctttcgcttt
    12001 gatcttcaca tgatgtggat ttttcatgaa tttaacgcca atattcttga tagctggcgg
    12061 catcgttgct gagaaaagta atgtttgtct ttgatcagga acttgtgaaa tgattttttc
    12121 gatatcttcc aagaagccca tattcaacat ttcatctgct tcatccaaaa ctaatgtttg
    12181 aacggttcca agtttcaatg tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc
    12241 cacaacgata tgtggacgat ctttcaagcc acggatctga cggccaatat ctgctccgcc
    12301 gtaaacggct tgtacacgta cttttttgtc gcgccctaaa cgatataatt cttcttgtgt
    12361 ttggatagct aattcacgtg ttggtgcgat aactaaacct tgtaattcgt gacgatctgg
    12421 atcgattttt tctaacattg gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc
    12481 ttgtccaata acgtcttttc cagctaatgc tagtggaatc gtttctgctt ggataggcgt
    12541 tgcttcttca aaaccagcgc gttcgactga tttcaacaat tctggtgata attctagttc
    12601 tttaaatttc aaatgaatcc tcctatatgg ttgttaactg aggatgtagc ttttttgcta
    12661 ccccgtcgat ttgttcggtc aaagtgtatt gtttcttttt gacagtggcc acgcacgttt
    12721 ttatccattc ttagcccctg tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta
    12781 gaaaagccaa acgggtcgcg ttcagcttta tcattattat gacatctggc ttcaacaagt
    12841 tgtttctatg gcaaaaccag tccaatttct cttcatcttt cggcaatagg ttattctaac
    12901 atagtttttc gttttctgca agtttacaga tttttatttc atttgctgaa gttgttctac
    12961 cacttcaaat aagcccatgc tgttgctgcc ttttagcata atactatctg ttgcttcaag
    13021 attttgtttg atagcatcaa tcaatttatt cttttctttt tcaaaataga acacaggtag
    13081 ttgggggtat ttttggttca attcttcttt tagtgcatgc atttgttccc cataaaggaa
    13141 aatcatggcg aatttctcag gatcgatatg ttcactcatt tgcgcatgca tatttaaaga
    13201 ctctttccct aattccagca tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag
    13261 atttttaaaa gtatctaata ccagtcccat ggctgttgga tttgcattgt agacatcgct
    13321 caaaatagct gcaccatttc ctgccgttag ccattcggtt ctgttttgag tgatagaaac
    13381 atgggcgagc ccttggcgta tctcaggtat ggacagtcca aagaagcgcc cgatcgtata
    13441 cgcaatcaaa gcattcgtta cattataact tccaggcagc gggatcgtaa atgtttctcc
    13501 ttctactaca aaatcggttt gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc
    13561 atttccaata ccgaatgttg tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa
    13621 cggttcatct gcgggcacaa cgagtaatcc gtctgataca agtccgtcag tgatttccat
    13681 tttggcttta gcgattcctt ctcgcgaccc aagattttct atgtgtgctt caccgatcat
    13741 tgtgattgct gcagcttttg gttgagccat aagtgaaagt tccgtgattt cattggcgtg
    13801 atccatgccc atttccaaaa cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta
    13861 aggaagacca atattattgt tgtaatttcc ttgcgtctta tatgttttgt atttttcaga
    13921 caagacagac gcggttagat ctttcgtggt ggttttacca ttgcttcctg taattgcaac
    13981 gacttctgcg cctacttttt ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg
    14041 atctgctaca ggaataactg gaaaattttt tggagcttgt tcaacaggca agctccaaaa
    14101 tgaagcagat gctccgtttg tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc
    14161 agcaagagga acgaataaac ttccttcggt tatcttccgg ctgtcaaatt cgacattggt
    14221 aatctttgtc agtgcagagc attcgctgcc cacggcttgt gcaatttctt gtattgttag
    14281 attcattgag atctctccta aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt
    14341 tcgcatgact ctcacttcca agtaaatttc tctattcgta tttagaagct agaatgaacg
    14401 cggttacgca atcactccag cctcttcttt atttctcttt attcattttt ttcaaaaaaa
    14461 gattgacgct gatggtatcg attcattcct aactggatca gttcttcaat caaatcaccg
    14521 tatttcaagc ccatattttc ccataagagt gggtacatac tgaactccgt aaatcctggc
    14581 atagagttta attcattcag gaataattca tttttatttg tcaaaaagaa atcgcaccgg
    14641 ctcaatccgc ttccacctaa catcgtgtaa gctaacttcg cgtactcttg cgctttttga
    14701 taaacttctt ctggcacttc ggctggaatc tgcatttcga ttttattatt gatatatttt
    14761 gcttcataat catagaatgc tacgtctttt acgacttcac caggcaaagt cgtccgaacg
    14821 tcttcatttc ctaatacagc aacttcgatt tcgcgcgctt caattccttg ttcaacgatt
    14881 gctcgagaat catactgata ggctgttgct aaagcatttt gcagctcttc tcggttttct
    14941 gcttttgtaa tgccgacact agaacccata ttcgccggtt tgacaaacat cggataaagc
    15001 aaagaacctt cacattgatc aaatactttt ttaggatttt ctttccattg attcttaagt
    15061 actggtacat aaggaacttg cggcacacca gcagcttgta aaatatactt ggtcatgatt
    15121 ttatccatgg cacatgcact ggtcaatacg cctgcgccga cataaggcat attcaatgtc
    15181 tctaagaagc cttggatcgt tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg
    15241 atggctcctt cttctttgat ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct
    15301 gtctgtccac ttgggtccca tgaaagatgc aagacatctt tgctggcagg tttttctgtt
    15361 agtaatggac ctttgaccca ttgtccttct tttgtaataa atacgagttg aacttggtaa
    15421 taattataat aaatggcatt taaaactgaa aatgcggaaa gaatggacac ttcatgctct
    15481 gcgctgcgtc cgccatatag taaagtaatc ttcaaaagat tgtcctcctg tattctaaat
    15541 cgttcagtga tttactctca tattttagca caaaacaaaa ataaacgaat aacttattta
    15601 taaaaaaacg agccagcttc taaaagaacg agcgtcattt attttttgtc agctagatta
    15661 tttgtcccgt tttttataaa aagttatgat aaagaaaagg agggatgatg atggcgcaaa
    15721 caaatccacg cagccgcttg gcagaaatca ttcgagtgtt catcaattac aatgtagtgc
    15781 caaactttgt acagcaaaaa aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc
    15841 ccaccttcat caagatagga cagatgcttt ctgttcgaga agatttgctt tcttctgcct
    15901 tcactcaaac attcaaaaca ttacaagata gtgtacctag cgacacattt tcaacggtca
    15961 aaaagacgat cgaaacagaa ctttctcttt cattatcaga tatattcgat gatttttcaa
    16021 aatcgccttt tgcctctgct tctatggggc aggctcatcg tgccaagctg aagaacggtg
    16081 acgcggtagt cgttaagatc caacacccta acatcgccga agagatccga cttgatcttc
    16141 aactatttga acgagcgatc cctttgatca aatacattcc tgaaactagc gtagttgatc
    16201 taaaaggggt actccaagaa gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag
    16261 aaagtcaaaa tggcgaacaa ttctatcaaa agaacaatgg ctggaaagag atccgcagcc
    16321 ccaagattta tgacgctttt tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta
    16381 aaaacttgaa tcatttaatg aacgccgaaa acaaaactga aacttttatc actggaattc
    16441 aaaacaagca gctgaaacaa gaagttgcaa agcttttagt tgagaacttt atgaaacagg
    16501 tgtttgatga tggttttttc catgctgatc ctcatccagg gaatctgcta ttccatgtac
    16561 taacaaaaga agaacagaca caagctagcc gaaaaactga aacagtccat gaaaaagagt
    16621 tcggttcctt tgcttttcga gcctccactt ctgcagaaga ccctgttgcg ccatatacca
    16681 tcaactacat cgattttggt atgatggggc atctttctgc aggacttagg caaaaactca
    16741 cacaagccgt tttagcgctg tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc
    16801 gactttgtca gcaagaaggc tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt
    16861 ttcttgaaca gtattatgat tctcctattg atgaaatcaa cttgcaagaa gtattcacac
    16921 atgtcgtgac gatttgtcat caaaataatc tgcagttcga tcgtgatatc acgcttttgc
    16981 tgaaagcatt tgggacatta gaaggggtga tccgcgtcct cgatcctgaa gtttccctca
    17041 tggaagtcgc ttcgcctttt gctcaacatt attttctcac tcatctggat gtagaagata
    17101 cattgaaaca aagcggatta gacttattgg aagggatgaa ggctgcgcct aagatccctc
    17161 aacagctcca ccatctatta gaaatgtgga cttcgggaca aggaaaagtc aatttggaat
    17221 tgaaaaagca agacaaatta ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg
    17281 gtatgatttt agctgcattg atcgtagggt cctccctgct tgtccaagca gcacctgtag
    17341 aaaacgcaga ggtcgtttcc ttattaggga tatttacgta tgctattgcc gcattcgtca
    17401 ttattttttt ggcaatcgat gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa
    17461 aggttgtgac agaaagaatc agcttcaaga aataagaagg ggttttacat aaattgtttc
    17521 tcaaattttt gtgaaaattc ggcttatttt tcgaagaggc tacttctgaa acaccgagaa
    17581 taaaaaatgt gaaaaagctg ttctgcaccc agtaataaga acaatcaaaa agggaacatg
    17641 gaacacattt atcctgtttc cctgttccct ttttgatgta ttcgagcata aataaaatac
    17701 cttcgttacc tactatcttc tgtacttggt aaaccttagt caattgcctt ttttaacgct
    17761 tgacgaaaag acatatgatc tttctccgta aagggttttg gaccctttgt ccttatgcca
    17821 gcttttctta actttccact atgataacgt tgttctagaa gcgtgccgat attcgcttcg
    17881 tcaaattctt ttccagagtg atggaacact cgcacaccct tcggcaatac aagtgaagct
    17941 aaaccataat cttgcgtgat caaaaagtca ttcttcttaa ccataccgac gattttataa
    18001 tccgcactat cagctccgcg gtctacgtag acgatttgga catgattagc gtattctttg
    18061 gtcgtataat ggtccacact ggtcacgata accacgtcaa gaccatagga ttctcctaat
    18121 tttatgacat cttcttttac tggagaacca tctccatcaa tgaatagcct cattcttttt
    18181 cgccttcttc cataaatgat aaacagttac taatccgctc agtaaaagac caacattgat
    18241 ccattttgcc aagataagca gttgtacact ttctatcggc actgattcat ggttcagcgt
    18301 gatcaataat agatggattg gctctcccat ttcttttgct aaaacaaaca aaagcgtcat
    18361 caataaaaac tggtggatca cactcccttt attttgagta gtatacttga caacgatccg
    18421 gcgatccatt ggaatagctg tatcagtttg ataatattcc ttatttagcc agctgcctgt
    18481 tactacgtgc cagaaactaa gcagaaagac acctatcgca aaaccatttg gaatcaagag
    18541 gaccaataaa gaagaactca gtccaagcaa ttgcatagcc aatggagacc atgttaaacg
    18601 tttcaaaatc catggtccga ctatttttgc tgcaatcaag cctgctgcat aaggcaaata
    18661 cataaaaatc gaaagttttt ctaatccgta cagtatgcca acataaaaac tgctaaataa
    18721 aaaaaggaaa ttccctagca ttccatttag aaaagtaaac agatttagcc agcttggcaa
    18781 cttccattcc ttcttctggt gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa
    18841 accataaata gcaagatcaa aaagcctttc atcgaataac aatcgggcat ttctcaaaag
    18901 aaacaataag ccaaagaata tcaaaaataa aacaagctct cgcgtagcta ccagatttct
    18961 tttgatgtct ttgaaatcca gttcgtaacg cggataatga ctaaccgtat ggtaggccat
    19021 gacgtaaaaa agtgtataaa tagtaaagct tgctgcaatc tgagtcggtg caggcaactc
    19081 aatcgcttga tacaggagaa atagccagat agctgcaaat aagtactttt tcccatccat
    19141 attgataaaa ccttgcgtct tttcgtgaaa attgaccgtg gtattagcag gtacaagcca
    19201 tgctgcactc agccctagca aaacacttga aaaataaaat aaaggaaaat aaaatacgcc
    19261 taatattcca agcaatgaac cagcaccacc aatcagcaga gcgatcatca gcaacgtgta
    19321 actatctaga ccaaatcgta gactgttgac taaaaatagt ccagtcatcc gaaaggcata
    19381 aaacagcgta aatggcaaaa tcgtcagcca attatgcgag tctgttagtc ccaaaaataa
    19441 aagataagga ataaaaataa ccgtgttact taagataaag ggcgttgttt gtgtaaaatt
    19501 acgcccaaat cgtttgagtt tttcgaacat gacaatggct ccttaaacag tcttataact
    19561 aatctgctct tcgattttag cattaaaggg gaaataaacc aattttacag aatatattct
    19621 tgtaaaaaaa aatacagaaa gaagcataag cttctttctg catttttacg gtacttgatt
    19681 actcaagcag acaagccctt ctccacaaaa gtcttgcccg acacccctgc atccggcagg
    19741 tgataaagat agaaatcaat tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc
    19801 agagtccttc tcgacccgtc gcataaattt actttaacac agttcctatg tttatgcgag
    19861 agcttttttc attttataat cgaccatttg aaatccttga ccgatatctg aaataagttc
    19921 ttctgtaatc tcaaatcctt catgcttata aacctcgatt gcttgatgat tcccctgatt
    19981 cactcgcaaa aagatctctc tttcctttgt ttgcgcagca agttcttcat accagcgaaa
    20041 aactgaagaa gtcaatcctt gaccacgtac tttatttagc aaatataatt tacttaaata
    20101 aatttttcca tttatctctt cataagcagt gtaaccgaca gtcttttctt cgctttttaa
    20161 taaaaagtag gacacacctt ctgtcagttc tttttgaatc tgtttttttg actgataagt
    20221 tttcagcatg taatccactt gtttttgacc aatgatcggc acaaaaactt ccggccatac
    20281 ttcttgtatg actgccatca gctcatccag ttcattttct gttactttta ttttttccat
    20341 acacccacgt ccttttttac attataaatg accttgcgct tgatggaata aaaagagcat
    20401 gatcatgcta tacacaccca ccgaccatgg tttcaagaaa atcacaatct gcataaattc
    20461 cctaaaagat aatttcgtca aaccagctac tagacagatc aagtcgtctg gcgcaaaggg
    20521 aagcagcaag gtaacaacta gtactttttt aatatctttc gtttttcttg tcagcaattc
    20581 atcaaatttc tgatactttt ttggcgataa aataagctgg acaaaggcta cgccatagta
    20641 acggacaagc aaaaatccga ttgcttcccc aatcactagg ccaatacaac tatataaaag
    20701 cccaaatcca ttgccaaaca tcagcatgcc cgctactgag gagatccctc ccggcaaaat
    20761 aggaatgatc acttggatca cttgcaacag aacaaatatc atcactgcat acttgccaaa
    20821 ttgctggata aacgcttgca aagacgtgct cgatcgaaaa atcccatgat gataagcata
    20881 aagaatcaac acaaagaaaa aaaacagacc aataatgggc atgatctgaa gacatttttg
    20941 taaaactgat ccttttttag acatctatat tccccacatc ttttttcatt tattaaaact
    21001 gcctcct
//
LOCUS       DAZULG010000046        20423 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000046 DAZULG010000000
VERSION     DAZULG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.047"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="ACG9CY_001476"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="ACG9CY_001476"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS4937991.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="ACG9CY_001477"
     CDS             1011..2258
                     /locus_tag="ACG9CY_001477"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS4937992.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="ACG9CY_001478"
     CDS             2334..2678
                     /locus_tag="ACG9CY_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937993.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="ACG9CY_001479"
     CDS             complement(2795..3121)
                     /locus_tag="ACG9CY_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4937994.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3736..4938)
                     /locus_tag="ACG9CY_001480"
     CDS             complement(3736..4938)
                     /locus_tag="ACG9CY_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4937995.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5055..5570
                     /locus_tag="ACG9CY_001481"
     CDS             5055..5570
                     /locus_tag="ACG9CY_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4937996.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5769..6215)
                     /locus_tag="ACG9CY_001482"
     CDS             complement(5769..6215)
                     /locus_tag="ACG9CY_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4937997.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="ACG9CY_001483"
     CDS             complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="ACG9CY_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4937998.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8094..8723)
                     /locus_tag="ACG9CY_001484"
     CDS             complement(8094..8723)
                     /locus_tag="ACG9CY_001484"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4937999.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8707..9171)
                     /locus_tag="ACG9CY_001485"
     CDS             complement(8707..9171)
                     /locus_tag="ACG9CY_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4938000.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9658..10926)
                     /locus_tag="ACG9CY_001486"
     CDS             complement(9658..10926)
                     /locus_tag="ACG9CY_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4938001.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11157..11894)
                     /locus_tag="ACG9CY_001487"
     CDS             complement(11157..11894)
                     /locus_tag="ACG9CY_001487"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4938002.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11961..12530)
                     /locus_tag="ACG9CY_001488"
     CDS             complement(11961..12530)
                     /locus_tag="ACG9CY_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS4938003.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12658..13530
                     /locus_tag="ACG9CY_001489"
     CDS             12658..13530
                     /locus_tag="ACG9CY_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4938004.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13872..14744
                     /locus_tag="ACG9CY_001490"
     CDS             13872..14744
                     /locus_tag="ACG9CY_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4938005.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14741..16699
                     /gene="nagE"
                     /locus_tag="ACG9CY_001491"
     CDS             14741..16699
                     /gene="nagE"
                     /locus_tag="ACG9CY_001491"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS4938006.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16950..17801)
                     /locus_tag="ACG9CY_001492"
     CDS             complement(16950..17801)
                     /locus_tag="ACG9CY_001492"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4938007.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17924..18391
                     /locus_tag="ACG9CY_001493"
     CDS             17924..18391
                     /locus_tag="ACG9CY_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS4938008.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18654..19136)
                     /locus_tag="ACG9CY_001494"
     CDS             complement(18654..19136)
                     /locus_tag="ACG9CY_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4938009.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19236..20099)
                     /locus_tag="ACG9CY_001495"
     CDS             complement(19236..20099)
                     /locus_tag="ACG9CY_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4938010.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6140 a         4204 c         3703 g         6376 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
     3421 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
     3481 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
     3541 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
     3601 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3661 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3721 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3781 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3841 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3901 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3961 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     4021 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     4081 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     4141 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     4201 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4261 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4321 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4381 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4441 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4501 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4561 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4621 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4681 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4741 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4801 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4861 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4921 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4981 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     5041 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     5101 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     5161 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     5221 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5281 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5341 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5401 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5461 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5521 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5581 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5641 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5701 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5761 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5821 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5881 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5941 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     6001 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     6061 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     6121 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     6181 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6241 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6301 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6361 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6421 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6481 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6541 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6601 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6661 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6721 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6781 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6841 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6901 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6961 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     7021 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     7081 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     7141 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     7201 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7261 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7321 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7381 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7441 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7501 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7561 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7621 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7681 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7741 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7801 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7861 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7921 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7981 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     8041 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     8101 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     8161 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     8221 aactgatctg cttcgactcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8281 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8341 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8401 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8461 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8521 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8581 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8641 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8701 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8761 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8821 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8881 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8941 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     9001 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     9061 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     9121 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     9181 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9241 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9301 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9361 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9421 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9481 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9541 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9601 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9661 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9721 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9781 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9841 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9901 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9961 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
    10021 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
    10081 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
    10141 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
    10201 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10261 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10321 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10381 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10441 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10501 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10561 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10621 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10681 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10741 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10801 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10861 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10921 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10981 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct
    11041 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    11101 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    11161 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    11221 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11281 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11341 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11401 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11461 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11521 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11581 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11641 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11701 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11761 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11821 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11881 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11941 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    12001 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    12061 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    12121 tttttcttga tgtggattgg ctttactaac aagcctcttc gttcttttgg atcggttaga
    12181 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12241 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12301 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12361 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12421 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12481 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12541 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12601 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12661 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12721 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12781 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12841 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12901 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12961 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    13021 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    13081 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    13141 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    13201 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13261 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13321 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13381 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13441 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13501 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13561 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13621 atttcttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13681 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13741 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13801 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13861 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13921 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13981 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    14041 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    14101 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    14161 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    14221 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14281 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14341 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14401 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14461 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14521 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14581 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14641 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14701 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14761 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14821 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14881 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14941 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    15001 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    15061 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    15121 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    15181 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15241 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15301 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15361 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15421 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15481 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15541 aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg
    15601 tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca
    15661 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15721 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15781 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15841 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15901 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15961 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    16021 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    16081 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    16141 cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa
    16201 caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat
    16261 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16321 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16381 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16441 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16501 atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16561 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16621 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16681 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16741 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16801 caactccgcg gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac
    16861 tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact
    16921 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    16981 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    17041 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    17101 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    17161 tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    17221 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    17281 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    17341 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    17401 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    17461 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    17521 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    17581 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    17641 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    17701 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    17761 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    17821 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    17881 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    17941 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    18001 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    18061 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    18121 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    18181 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    18241 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    18301 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    18361 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    18421 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    18481 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    18541 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    18601 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    18661 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    18721 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    18781 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca
    18841 acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc
    18901 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    18961 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    19021 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    19081 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    19141 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    19201 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    19261 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    19321 gcttcgacta acccataatc tgaagaagga agccattctg catagatttt cccccaagta
    19381 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    19441 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    19501 tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt
    19561 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    19621 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    19681 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    19741 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    19801 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    19861 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta
    19921 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    19981 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    20041 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    20101 tttatcttcc tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    20161 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    20221 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    20281 aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc
    20341 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    20401 gaacgctgct tggatttttc ttt
//
LOCUS       DAZULG010000047        20336 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000047 DAZULG010000000
VERSION     DAZULG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.048"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1443)
                     /locus_tag="ACG9CY_001496"
     CDS             complement(<1..1443)
                     /locus_tag="ACG9CY_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4938011.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYS"
     gene            complement(1501..2058)
                     /locus_tag="ACG9CY_001497"
                     /pseudo
     CDS             complement(1501..2058)
                     /locus_tag="ACG9CY_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(2206..2372)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(2597..2803)
                     /locus_tag="ACG9CY_001498"
     CDS             complement(2597..2803)
                     /locus_tag="ACG9CY_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938012.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2819..3433)
                     /locus_tag="ACG9CY_001499"
     CDS             complement(2819..3433)
                     /locus_tag="ACG9CY_001499"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS4938013.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(3580..4263)
                     /locus_tag="ACG9CY_001500"
     CDS             complement(3580..4263)
                     /locus_tag="ACG9CY_001500"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4938014.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(4372..4830)
                     /locus_tag="ACG9CY_001501"
     CDS             complement(4372..4830)
                     /locus_tag="ACG9CY_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS4938015.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4914..6677)
                     /locus_tag="ACG9CY_001502"
     CDS             complement(4914..6677)
                     /locus_tag="ACG9CY_001502"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS4938016.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6898..8358)
                     /locus_tag="ACG9CY_001503"
     CDS             complement(6898..8358)
                     /locus_tag="ACG9CY_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938017.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(8566..9492)
                     /gene="glsA"
                     /locus_tag="ACG9CY_001504"
     CDS             complement(8566..9492)
                     /gene="glsA"
                     /locus_tag="ACG9CY_001504"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS4938018.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(9515..10942)
                     /locus_tag="ACG9CY_001505"
     CDS             complement(9515..10942)
                     /locus_tag="ACG9CY_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4938019.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10973..12187)
                     /locus_tag="ACG9CY_001506"
     CDS             complement(10973..12187)
                     /locus_tag="ACG9CY_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS4938020.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            12537..13616
                     /locus_tag="ACG9CY_001507"
     CDS             12537..13616
                     /locus_tag="ACG9CY_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4938021.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(13917..14159)
                     /locus_tag="ACG9CY_001508"
     CDS             complement(13917..14159)
                     /locus_tag="ACG9CY_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4938022.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(14191..14748)
                     /locus_tag="ACG9CY_001509"
     CDS             complement(14191..14748)
                     /locus_tag="ACG9CY_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4938023.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(14761..14949)
                     /locus_tag="ACG9CY_001510"
     CDS             complement(14761..14949)
                     /locus_tag="ACG9CY_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4938024.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(14962..15528)
                     /gene="amaP"
                     /locus_tag="ACG9CY_001511"
     CDS             complement(14962..15528)
                     /gene="amaP"
                     /locus_tag="ACG9CY_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4938025.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            15781..16269
                     /locus_tag="ACG9CY_001512"
     CDS             15781..16269
                     /locus_tag="ACG9CY_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS4938026.1"
                     /translation="MTRFYLFIKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNF
                     DWQTTLFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGF
                     AFFGLLPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDL
                     TV"
     gene            16278..16505
                     /locus_tag="ACG9CY_001513"
     CDS             16278..16505
                     /locus_tag="ACG9CY_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938027.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            17141..17512
                     /locus_tag="ACG9CY_001514"
     CDS             17141..17512
                     /locus_tag="ACG9CY_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938028.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(17768..18010)
                     /locus_tag="ACG9CY_001515"
     CDS             complement(17768..18010)
                     /locus_tag="ACG9CY_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4938029.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(18042..18944)
                     /locus_tag="ACG9CY_001516"
     CDS             complement(18042..18944)
                     /locus_tag="ACG9CY_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4938030.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(18957..19145)
                     /locus_tag="ACG9CY_001517"
     CDS             complement(18957..19145)
                     /locus_tag="ACG9CY_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4938031.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(19159..19722)
                     /gene="amaP"
                     /locus_tag="ACG9CY_001518"
     CDS             complement(19159..19722)
                     /gene="amaP"
                     /locus_tag="ACG9CY_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4938032.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(19760..>20336)
                     /locus_tag="ACG9CY_001519"
     CDS             complement(19760..>20336)
                     /locus_tag="ACG9CY_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4938033.1"
                     /translation="LPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAEL
                     PLEQVKDTFMINIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASI
                     ANFTIGLAKQLAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGE
                     LAPVYVFLASNKASYVTAQIYGVTGGEAINL"
BASE COUNT         5756 a         4223 c         3546 g         6811 t
ORIGIN      
        1 ggaatagatc aaagcagttt ttaagtctac ttgtgataca tcactactga cacgcgttgt
       61 tttaaaagat ccccaagaac tgtctttttg ccatttaagc ccgttgatcg tcaacacttc
      121 atttggatcg atagtgccgt tgtcgagacc gattgctgct gtaatcattt taaacgtaga
      181 tccaggagca taacctgtcg caaatcggct gatgaatgga tgttcaggat tttcttcata
      241 agctttgtaa tcttcttgtg agatcccgtt tgtcattttg tttggatcat agcttggaga
      301 gctagcaagc gcaagaagat caccggtttt tggcgttgtc gcaacagttg atgcagcttt
      361 tcctcctaga ctgtcaaaag ctattttttg tgccttcgca tcgattgtca atttgatatc
      421 tttgccattt tgaacttcat gttcgatcag aacttttttc tctacaccgt ctgcatctgt
      481 aatgcttaat ttcccacctg ttgtcccacg aagatcctta tcaaaagcca tctccaaacc
      541 tgagcgaccg atttttccat tactgcttaa ttctggattc ttatcaatat cttctgctgt
      601 gatatctccc acgtaaccaa tcagctgagc agctgcttca cccaaaggat aatatctgcc
      661 gtctacttct tgaatggtag ctccagctgg aagttctggc gttgctccat caatgatttt
      721 caatgggaca aagtaatcgg gttgtaccca gctttgtgaa atcgcctgat tgatagcatc
      781 ttctgttaag tcgaaggagg aagcaatcgc tttgatattg gctgtttttt cgcctccatc
      841 tccaagttta cttggaacga ctcctaattg ttttagtttg cctgttgttg ctaatggttc
      901 accatttcga tctataatat tccctcttgc tgcttcttgt gtggtcaggc ttactttgtc
      961 atttccttcc atttctggaa aaaccaagtt gggttgccag ttgatcgtgg tttgaccatc
     1021 atttctgtct aatgttcctt tataagaaag atctttcaat tcacctaatg aggtattcat
     1081 ctttgcttta tatgaaaaac tgtaagtaga atcgtctttt ttatctactt ttagattcga
     1141 tatctgaagt cctttgacat cagcagcacc gtatatattc tggtatttat ctaagatctc
     1201 tttttcagat aatgcacttt tatttgccgc cttttttgat gtcatttctg cagctttgtt
     1261 ataatctccc ttgttcaaag cttggacaaa ttgctcaact gtcttttctc cagcttctac
     1321 tgcttgggtt tcttgatagt gctggtagta aaaataaccg ccacccgcag ctgctattaa
     1381 gatcactgcg ccggcaatat aagcgcctgt tcgatttttg ccgtgcttgt cacttctttt
     1441 cattttttcc tccttctctt gcttgtcatt cctttatttc actatttata cctgtttatt
     1501 ctaacatatg tctttgagga aatccatacc acaatccaga agcacacttt ctggaaggtt
     1561 cgtttggata ctaccaagta atttcccaac cgctttgggt agcttgaata ttgaagttac
     1621 gtctttcagc tgctgagaga tagctttgat cacttgctgc tgccttctga tcctcccgaa
     1681 gtctccttct ttgtcttccc gaaatcgagc atactggagt aaagtattac catccatcac
     1741 ttgctctcct ttgtttatat atacgccatc taaatctaga gatttttctg catcgatctt
     1801 gactccacca ggaaacagtt cattaacgca atcgataaaa tcttgaaatg tgatacttgc
     1861 ataataggga gcttcaaatt tgaaattttc ctttaatgtt tgatttaata aatcaactcc
     1921 cccataagag taagctgcat ttatcttatt cattccgtaa cctggaatat cgacatatga
     1981 atctctcatg atagagatca gttttgcttg ttgtgttttc tggttgtatt gtgcaaccag
     2041 aagtgaatcc gagcgacctc caccaatcga tcttttttca ccgcatctcc tcctcacaca
     2101 gcaatccact tgtcaggtga ggagcgagtt tgtctcatca aataagtcag tcacaaaaat
     2161 ataaagaaaa ggtcagaaac cttttcgaac aagaattctg acctagcatc atctcatcgt
     2221 ccgagtttta gcactataca acgtaaagaa aacattctta gctattggaa aaagccttaa
     2281 tccaacagtt tctgttttac ccattggcga ctttcgacgt ttctgggcaa cagcctatgt
     2341 ttgtatagga gcctcaccta acaaggttat tactatattc tatttacgag ttcatgatag
     2401 acttttccta atgattaggc aatggaattt gaaatgaaat agtgaaattt aacaatcttt
     2461 aatatttttt agaaaaaaat aacaaataag ttgaaagaat cccgttgcaa attctttcag
     2521 ttgcaccagg attctttgat aattttttag ttttatgtgt ttttttatat gttttttcac
     2581 ttttcggaat aaatatttat ttgttaaagt aattaaacaa tccgctaatc gtttgatctg
     2641 gcatttctat ttccttacca tctaaagtaa cagtagtaac gacaaaatgg tcttgcaatg
     2701 gcggaacttc taagtactgc ccttgcagaa aatcattcac tgtttcatat tcttttttca
     2761 tttcttttga atctgtcgat ggtttatagc ttatttttaa cataactagt gctccttttt
     2821 attcgggacg tgcaatcgtg aaaatttctc ctattgctgc ataatcattt cctgccaaac
     2881 ggcttacagc attcaatttt cttggatcga tccttcctgt cttatcatca tagactgttt
     2941 catttaaatg atatttctgt acttctagca aaaataaatc agctgttact ttatttttat
     3001 tccgtatctc gacatgttca tataaggatg tttcaaaacg aacttttgct tctgtcaatg
     3061 ctggtactga aatgattact gaatcagtta aagtcaagtc agtcaggtac agttcgcttt
     3121 cctcattagg cagattggct gctgttttat ttacttcttc tacgttgtcc tcatcaagga
     3181 tatggataac agcttctctt gtctcaagaa tattctttgc tgtgtctttt actgcatctt
     3241 gttttctctg gatcgccaag gataagatgg gtcgattcga actgacgata ttgaagtaac
     3301 tgaagggtgc gatattcact atatcttttg ctgatttagt cgttactact gctactggtc
     3361 tagggatgac tgaaccaatc agcaacttgt aattttcacg ttctgtcaat tctgaaggac
     3421 tccgttcaaa catggtttcg ccttctttct ataaaattcg ttaaaaagca gatcttgaag
     3481 acaaaatgaa aagtaatgct tttttcatct tcttagaatc tgctttaaac tatctatcaa
     3541 tttttctttt ttgtcaagaa tctgtgccgt gcgtgttttt tagttatttg ctttacttgc
     3601 ggcttcgaca tatcttttag tgatcaattg cggacgggtg ataatagatc caactacgac
     3661 tgaaaaagca cctaattcat atgctttctc tacttgttca ggagagtcag tatgtccttc
     3721 cataattact gggatatcga cttgttctat gatttgattc aataaatcaa aatcaggtgt
     3781 ctgcgtcttt cgtgaatatt ctgtatagcc tgctaaagtc gtagacaaaa gatcaaaacc
     3841 atcttcttgc gcttgaagcg cttcctcaaa agtcgaaata tctgccatag ctaaccgacc
     3901 gtgttcgtga atcatttgga caagttcagc agttgttttt tctgtcggac gtttacgaga
     3961 agttgcatcc agtgcaatga tttctgcttg cgtctccaaa agttcttcca cttctttttc
     4021 tgtcgcagta ataaacactt ctgacccatc gtaatctctt ttgataatcc ctattaccgg
     4081 taaatcaacg attcgcttga tttcattgat atcttctgtc gtatttgctc gaataccaac
     4141 tgctccgcct tcttttgcag ccaaagccat tctccccata ataaaagaac tgtgtagcgg
     4201 ctcgttttcc aaagcttgac aagaaacgac gactccgcct ttgatttgtt ccagtgtatc
     4261 cacaatatct cctccagtaa tgttatataa aaatacagct tcatataaaa atgtaaccct
     4321 tttctattca ggagacaata atgagctatt tctttcagca gacctttctt actaaaagaa
     4381 aattacttca aattcttcgt atacgaccag cttctcttct gtaaattcta tgttcaacgc
     4441 ttctttataa gcatgtttaa aaaactcagc atggactttc cgccaataag ctaatgtccg
     4501 atccccttct ccttctttcc aagcatgctc tttcgtgact tcactaaaag gcaagatggt
     4561 cacatttata tttttgatga tacaaactgg ttcatttttt ccattcagga ccacgtctaa
     4621 ttgaccaact tttggtaaag gctcattctc ttgtttatac agatcgtaac cacttgatgt
     4681 accagtcttt atctttttta caactagttc gctcaactcg tctgccattt tttctgtatc
     4741 cccaaatgaa aagaaaccta atttttctga ttgataacta ggatattttt cttggaactt
     4801 tttgacatat tcttctactt gctgatacat aacatccacc cctttgcttt ttataatgaa
     4861 aaaaccatcc agatactagt ataaacaatt atctggatgg tgagcataat gaattattga
     4921 atattcaaga cacgcagact atcttcaacg atttgttttg gtgttaaatg ataacgttca
     4981 taaagctgcc ctacaggaac gctatccgtg aattctttct ctgcaccaaa atttaatacg
     5041 ttcattgaag aatcaccata ataacgactg atattttcgc caaatcctcc tgacaaacta
     5101 ccatcttcta atgtgatgac taattcgtgt gtttctttta atttatctaa tgtttctacg
     5161 tcattttctg tgatcgttcc tggattgatc aacgttgctt cgattccttt ttttctaaaa
     5221 gcttttactg tttcttttcc taattcaaga aaaccgccta aagctaaaat tgctactttt
     5281 cctccattat ttactgtttg ataggctgga atactataat catttgcgat agctgtacca
     5341 tgttctacac catgtgtagg aattcggatt gctacaggct gcgctgtttg ttcgaacgcc
     5401 caatctagca tagagatcat atcctctttt gttgctggag caagatattt gatgttcggg
     5461 atacttgata agtaaggaat gtcaaatgtt ccttggtgtg ttcggtcact agctgagatc
     5521 gttccgccac cgactaaaat aatcactgga ttctcgttga tcgctaaatc atgtgataat
     5581 tgatcatatg cccgttgcaa gaaagtactt gattggaaga cgactggacg tactccctga
     5641 gaagccaaag cagaagcgaa agtgatagag tgctgttcag cgatccccac gtcaaaataa
     5701 tgatctggat gtttttcttg gaatgttttc agtccaaagg cgcctggtat cgctgcattg
     5761 actgcggcaa taggagctgt acttgtttgc attttcttat ccaaataatc catgatgatc
     5821 atattaaatg tttctccaga agttacttgc tttgcctccc cggtggacag atcaaatggt
     5881 acatgccaat gatatttcat tttattatcg attgccggct gataaccgcg acctttttcc
     5941 gtatggacgt gaagtacaat cggatgatca atatctttta cttcttcaaa taaacggatc
     6001 accgtttcca gatcgtttcc attatccacg taacgataat caaatcccat tgctttaaat
     6061 aggttattgc tgcttttacc gttggtttga cgaagctcag ctaagttttt atataaactg
     6121 ccatgattct catcaataga catctcatta tcgtttacta atacgattag attggattgt
     6181 aaaacaccac cattgttcaa tccttcaaaa gctaagccac cacttaggga gccgtcaccg
     6241 ataaatgcta ccacatttcc tgattctttc ttcagatctc tcgctttagc taaacctgtt
     6301 gctaagctga tagatgtaga tgtgtgcccc actgtaaaat agtcatgttt actttcatgt
     6361 tggttggaat atccgctcac ttcacggtaa tgtccttttt caaaaccatt ttttcgacca
     6421 gttaaaagct tatgaccata agactgatgg gaaacatccc aaacgatttt atcctttggt
     6481 gaatcaaata cataatgaaa cgcaatcgtt aattctacta cacctagatt cggaccaacg
     6541 tgcccaccta catcgctgtc cttttgcaag attttttggc gcatctcttc tgccaactgt
     6601 ttcatttcct caacagataa ttttttgaca tctgcggggc tattgattgc atccaataca
     6661 tgtaatactg tactcataat acattctctc ctccgtacta tcttctttta atgaaaatag
     6721 atttgttaca ctttatctat tctactactt agagttgact ctaagtcaat agcattttaa
     6781 aaaaatttca gagaaagctt gttatataag tagatcttcg cacgaatcag gactcaaata
     6841 aaaaccttct acagacaatt aaaaagcttt gtagaaggtc tcctaattaa ggtctcatca
     6901 agaaatatgt ttttttaata ggtgaagttc ctgagcaata tgatacaaat ggatgtttgc
     6961 accttttaat gagattggat agatctttgt cttttcagtt tctaatccat tttgatagct
     7021 agcagcatcg cacaaaacca ctcttttttt cgcacataga cattcatcag gcaattcttc
     7081 taatgaacga aataaaatag gatttatttc agcgatgcct tcttccgcat acaaatgaaa
     7141 ttgggttcga taaaaaaatt gtttgactga actttctgaa acaacaaaaa tttctatttc
     7201 ctgtctcccc gcttgttcac tgttgatcaa agaaagactt ttgacgaatt ttcgaatgaa
     7261 attttcattc catcttagtt ggatttgatt tttctttttc cactcttcca atatttctac
     7321 aatttttccg tacagaagtt gttctttttc tgatagaaga tagatccttt ccggtaaaaa
     7381 tacttgtcca tctgcccaca acgtacggac aaattgtaaa aaggattgct tgaaaataaa
     7441 attttctgca tcgtcgattt cagcgatact aataatctgt aaaatcaatt cattcagtga
     7501 taggtcattg agaacaaaat ttttataaac aacttcgaaa tcttttctta acagctccat
     7561 gttctcattg gtataatttc tactgttgaa caaagaaaaa atataataga catcttcttc
     7621 ttgtaagtct agaattcctt tttgctcttc caatccttga cagactaatt gaaataacgg
     7681 cttcgtttct gcgcttttct tatcttcttc tttcagtcca aggggaaatg cctttcttcc
     7741 gattgcgatc tctattcctc gtatgataaa ggacttttca tcttccgaat atcttctttt
     7801 aaaaaaatga gtctctacat aatcaatcaa ctcgttacaa gcttcttcta catacggatt
     7861 tttattttca tagccttccc aattgacata acgtactaaa gctagcaaga tgttccgcga
     7921 gtcaaattct gggataatga tttctttatt tttcaaataa tcattttctt taaaaaaatc
     7981 caatacgatt tttttgattg tatatacttt gctcaaagaa ataaattcaa gatcagccag
     8041 ttctgtactc gtgaatatac cattaaaata ataacttaaa aactttaaaa aataagattg
     8101 accgtacaga aactgtgtga gctcagaaag agcaatacca tgtttgaata cgagtttata
     8161 cttatgcttc tctcttactt gttcttttat acaatattct agacactcgg ctagactttc
     8221 tagatcgtga aggatcgttg gtgtagtcac ccttagagaa atagctgttt gttcgatgtc
     8281 tatttcttgg aattcaaata attgttcaca cagatacact tttctaaata tatttcgctc
     8341 gatgtaattt tccagcattc ctgatacacg cctccagttt ttcttatgat tttatttaat
     8401 catatctgta aatcgtattt ttgtttcctt tttatttaga aaagcccttt caaaaaccaa
     8461 taaaaaaaga aactgggaca aaagtcaaac aagctcacaa tttcagcctc attgtaggta
     8521 gctttgagcc gtttggactt ttaccccagc ctctttcaag aatggttatt caaagatgtc
     8581 agcgtctagt tctttgacta catctttcaa caattggata ccagctgcgc tgttaccaaa
     8641 tggatctagt gccgggctga agacacctat gccgtaacga tttggcacag ctgccatcaa
     8701 cccgccgcct acaccgcttt ttgcaggtac accaacatgg acggaaaact ctcctgattc
     8761 atcatacaaa ccagcagttg tcataattga tttgacgatt gtagcacttt cttcagtaat
     8821 gatttgtttg ccattccaag gagcaattcc tttatttgcc agcacagcag ctaattttgc
     8881 tatgcctgta gcggtgacat tgagggagca ttgtctgaaa taagcatcga gtatctccgg
     8941 tacgtctcct tcgatcattt gattaccttt catatagtaa gcaagagagc gattgatatc
     9001 acctgttctt gattcagaat gatagatttc ttcattcaat gtgatattag gatcatcaca
     9061 gatctctttc ataaatgaca agattcgctc aaatttttca tctgcatctt taccggcaat
     9121 caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga ttcattggat gtttgcgatg
     9181 attgatttcc atattcatga tagaattgaa ggcaaaaccg gttggttccg tgttgattgt
     9241 ttgaaagact ttttctattc cgttgtcttt gattgctaat aacaatacgg gaacttttga
     9301 catgctttcg atggcaaagc gcacatcggc gtctcctccc tcaatcagtt gttttgtatc
     9361 caaatcataa atcgccatac ctaattgttt gggatccact tcagctaatg ctggaatgta
     9421 atccgccact tgtcctgttt gataatagtg gaaatttttt ccaatcaatt gttctaattc
     9481 ttttacttcc atttgaataa ccagctttca ttttttattt ttctgcatgc tggaatttat
     9541 gacggcatgc gtagatcgta aatggaataa ctaagacgat aagataagaa acaatcagaa
     9601 tcgtttcata aatcccatgc tgtccagctg cgatactagc aggtggaaca aatgagatac
     9661 aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac gattactttt acaacttttc
     9721 cgcctggaac ctgataggct ctttcaagac tgtcatcttt aaaaaccagc ttcaaatagc
     9781 ccgcgaaaaa cataaagtac aacatcaggt agattacaac agtcaatgac atcgctgcaa
     9841 gaaatgaaag attattcccc ccgccgctta atgtaaagac ggctgcccag attgttgcaa
     9901 cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg tgtgttttct tttgcaaaat
     9961 attcaggaag taatccttct tctgctgcta cgtgaagtgc ttttgaagga gaggttaccc
    10021 aagaagcaat ttctccgatg acacctaaac aaacaagaat cgcaagaatt ttcgcaatcc
    10081 atgcaagatt tggatcaatg tgtcttaata ggaattctaa cgattgaatc acccctgtat
    10141 taagagacaa accatgttga ggtactgtag tagcgacggt caatccgcca aaagtatcta
    10201 aaatgattgc cagaataacc aatacaaaca ttgctaatgg ataatccttt ttagggtttt
    10261 ccatttcatt tgcatgactg gcagatgttt ctacgcccat atatgctaaa ataaaggaaa
    10321 caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat caatgttttc atagacagta
    10381 tcgtttgtag tggagcgcct gaagcgacat aatggatccc taagccaaac aagactaatg
    10441 ctggcaacaa gataccagct acaaaaccga tccgagcaat caatgaagtg tatttggttc
    10501 cttttaactg tgagagtaaa atagcccaga aaataatcaa taggattgcc aatttcagat
    10561 atttattgtt ttgtatagca ccaatacccg ttgcatagga taatgcacca gttaagaaat
    10621 acagcatggt caagtagcca actgttatct caaaccattg gaaaaagatt gcggcaaaac
    10681 cgaatcgttt tcccaatgtg cgactgaccc aagtatagac accgcctttt tcccatcctt
    10741 tcaccgttgc catctctgct gcgcacaatg ccacagggat aaaccatagc aaaccgccta
    10801 ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa tgtgggatat tcatataatg
    10861 aaacgaccat tgagatggtc atagccaaaa agccaaagaa cgttaatttt tttgcaggaa
    10921 ttttcgtttt gctttgttgc atgaatcttc ctcttttcta tttgaatttg tttcaattga
    10981 gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg ttcttctttt gtagtagtga
    11041 tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt cagcacatag gcatacacgg
    11101 cagtaatgat gacaccaatc acttgaatcc ctaagaagta ccagctgctt ggattcgtta
    11161 gtaaatggct gttagatgca aagatcccta tcagaatcgt acctgtgaat ccgcccatgc
    11221 catgaacacc ccacacatca agtgcatcat cccattgttt ctttttacga aaatcaacag
    11281 cagcatgaca gacgataccc gcaatgatcc caatcagcat ggcacttttt gcttctacat
    11341 aaccggcaca aggcgtaatg gctgccaatc cagcaacgga tcctgttagt acgtcgacaa
    11401 aatcaaagcc atttcctttt acttttgcaa tgatcagcca agtaatcatc gcaaatgcaa
    11461 gagcgatgaa tgttgaacca aatgcattga ctgcttgttc atctgctcga agtgcaccac
    11521 cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt accgatagca gctgccataa
    11581 gactgctatg ctgacttctt tctcctaaag gaatctttct ttgaccaagt gtgaggatac
    11641 atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc tccaccagcg aaatctctaa
    11701 atcccatctg atccaaaaat ccgccacccc atatccagtg gcaaacaggg aaataaatca
    11761 gcagattcca aaaaataaca agtataatat agccttttaa attcaatctc cctgcaaaag
    11821 ctcctgtcat caaaggaacg gtaatcacga taaacatcag ttggtaaaga aagaacatta
    11881 aaaatggaat cgttgcacca tgaaacatgt tggggaagct tgtaacatgt ctcatcaaaa
    11941 agaaatctgc cgggtttcca ataacaccgc cgatatcccg tccaaaggcc aatccgaatc
    12001 caccaaatac ccacataatc gttgttatcc caacggagag aaaagattga gacatgatcg
    12061 ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa agctagacca ggagtcatca
    12121 ggcaaaccat tgctgtacat aaaatcatga atgctgtatc tcctgtattg ataggtaatg
    12181 tattcatttt tttcgcctcc tttctacgat tacgagtata aaagaaggtg tagttgtttt
    12241 tttcttctgt ttttttgaat taatacttgc atttataaaa gacagacaat taataaaagc
    12301 ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa gacaaaaaat aaacggtctt
    12361 ctttttatag aaaaccgttt attttcgtct ttttttattt ttaaagatat tttttcataa
    12421 ggaataaaga ggcggatttt ataattaact tagccatttg gaaataaaaa aacgccacgt
    12481 gaattccatg ttatattgaa gttaccacaa ctctcaatag acaaggatga attcacatga
    12541 cgcaaaacca tgataacaca gtttcacgca agggaaaaca cctaacctac tcagaacgtt
    12601 gccaaatcgc catcctgaaa aaggaaaact attccaaccg tcaggttgcg gatgtcctgg
    12661 gctgcgtgcc gcagaccata aacaacgaaa tcaaacgcgg gaccgttact cagcttaacc
    12721 gccagaatca gaacgggaag gtctacgact attatcatga cgtctacgac gccgatgccg
    12781 ggcaggcggc ctacgagaag aaccgcttga actgcggcag acggccctta tgggccgaca
    12841 cggacgcctt cattgaatgg gcggacgaca tgatgctgtt ggaaaaatgg tccccggacg
    12901 tggtggtggg ctttgccaga aaacacgaat tgttcgaccc ggccattatc cctgggacca
    12961 ccaccctcta tcagtggatc gaccgtggca tcatgaaaac gaagaatatg gacctgctag
    13021 aaaaactgtc ccgcaaaccg aaatcgtccc gtcatggcaa ccgcccgaat aaacgggtgc
    13081 tagggaaatc cattgaagag cgcccggagg cggttggtac ccggaaaacc ttcggccatt
    13141 gggaagtcga cacggtcgta ggaaacaaga ccaagtcgga tgccgtgctg ctgacgttgg
    13201 tggaacggca gacacgcttc gaggtcattc tgaagctggc cgggaaggat gccgaatcgg
    13261 tcaaccaagc ggtccgacag ctgagagaac gggctggtgc gtacttcggc cagctgttca
    13321 agacggttac ctcggacaac ggctcggagt tcgcgggact gcacgatgcc ttgcaggatg
    13381 tggcaaacat ctacttcagc cacccctatg cttcctggga gcgcgggacg agcgaaaacc
    13441 aacacaagct gattcgccgt ttcatcccca agggcagccc aatcggcaac gtgagcgaaa
    13501 accaaatctt gcggattcag cgttggatga acgattaccc gcgaaagatt ttgggctatg
    13561 caacgccgca tgacacgttc gtccaggcat ttaggcagga acggttgacg gcttgacacg
    13621 tgggcccttg acaacgagcc tccctcggca tggcttgtgt ctggaaagct ggccggggcc
    13681 agccagtcgg gagactacca tgcctcagct cattgtcaag ggcccactcg attagaaaaa
    13741 ttagacggtt ggtggtaacg agcaaccggg tcaccatggg tggctaactt aaacttgaaa
    13801 tttacgtaag gaataaagaa tacttcatat aaaatatata aaaaagaacc gcagcgagtg
    13861 tcgctacggt tctcaaattt gttcttcatt tcaacataag tttttcttca atttgcttat
    13921 aatttacgtg caaagaatga aacgactaat actaaaatga ttgcaccgat caatgatgga
    13981 atgatagcca ttcctgcaat tgttggtccc caagttccta gtaaagcttg tccaattgct
    14041 gaacctacta aaccagcaat gatgtttgcg atgatcccca ttgatgatcc tttattcgtg
    14101 attgctcctg caattgctcc aatgataccg ccgacgatta atgcccaaat aaatcccatt
    14161 ttaaattcct cctaactgac ttcttacttc ttattgtaca cgtggttcgt tattttcttt
    14221 gactttttgt gcacctttgc ctacagcaga ttttgcttta tctgtttggc gtgaagcaaa
    14281 ttgtcctgtg gattttgctg cttctgttac tttgtcttgt actgtttctt gatctttttg
    14341 gtattcttct tgtgttttga tgtctgcaac attcacattc acttcgataa catctaaatg
    14401 tgtcatattg tttacttcag tactgatcaa ttctttgatt tggttgtaaa tatcttcgat
    14461 gtctttaccg tattctacga tcacatctag atccacagct acttgtttct tacctacttc
    14521 tgtattgata cctgctgtta cgttatctgt gttgactaat ttttcagcca tatttgagaa
    14581 aaatccgcca tctaatgtca atagaccatc tacatctttc aatgcataac cgatgatttt
    14641 ttggatgact ttgtcttcat aagtcaattc accattcatc atgcggtttt ctcctttttc
    14701 atttgccaaa actgatccat ttgttgtatt catgtttttg ttttccatct ttgtttcctc
    14761 ctatgattta aaaaattgat caactattcc tgactgtttc aaataaaaac cgattcctgc
    14821 gccaattgct gtcatgatta tgatcaacaa tgtcttgaaa aatccaatcg ttaaccataa
    14881 aacagctagc agtagtccaa gacccccaaa gataataggg agcttgtatg ctgaaaatag
    14941 ttctttcatt atttggccct cctattccac tcgagagtgc ttgtagtctc ttgtatcttc
    15001 ttgttcttca taaccgctgt atgtgactgc cactttgacc gtttcttttg ttccgagtac
    15061 tttagttact tcttcttcga tatcttgcat caatacacct gttttcccta ctaatgaaga
    15121 agttcgtttc aagtctccgt tgacatggat atggattcga tttttcgttg aacgtatccg
    15181 tactttaggt gaatgaatga actcttcttc gtgtaaatgg gaacgtacca acccttcgat
    15241 cgcttttttg tcgattgtta gtttgccatc ttcccgtttc ataacgaaag ttcctcttga
    15301 ctttggatag aaaagaacga ctagcaagat gataatagca agaacggcaa atactacagc
    15361 tgcccagaaa atatattgtt gcatgtagta attcgttaaa gtaaagaagc gaaatcgttc
    15421 caattgttca ggcataatat acggcgcatt gatcaacgca atcgctagaa atactgaaag
    15481 tagcagtaaa ctcatgataa caccgattat ttttaacccc ttattcattt tccacctcct
    15541 ataaacttat ttttaccctg taaataactc taccgacaat cacagtatag atagcgaaca
    15601 gttagcatag catcagtaaa taaagcgcta ccaaacattg ctggatcata acggcgtttt
    15661 attgccagtc tgatactgaa ataacaattg cttacttatt ctctatttac ttttgattgc
    15721 tggctatact taaaggatat tattcaagat taaaaattgc aaagattatg ctcagttatt
    15781 attacaagat tctatttatt tattaaaaca ttgctattaa aatttgtcgt tttaggtata
    15841 agtgccgtat ttattttgtt cggcgtactt ttttatactc agatcgctac aagcgaaaaa
    15901 ggattcaatt ttgattggca gaccacatta tttgtttctg tcatttactt tgcccttctc
    15961 ctagggatcg ttattgctta tttcttattg aaactattga aaaaaatcga agataaccaa
    16021 gctttttcca ctcaaagctt aaacatacta aaaaaaatcg aacgaaccat tttgtttatt
    16081 ggtttcgcct tttttggctt acttcctaaa tttatgaaag tagcggattt agacgatgct
    16141 ccaggtttga tgttggtagg aatcgctgtc gtattcattc cttttgcagt atacacatta
    16201 acaagcgtgc tggaaaaact gcttgaacat gcgattttac ttaagacaga tcatgatttg
    16261 acagtgtgag gtgcgacatg gcaattatcg ttaatttaga tgtagtatta gctcaaaaaa
    16321 aaatgagtgt gactgaattg agtgagaaag tgggcattac gatggcaaac atctctattc
    16381 taaaaaatgg aaaagcaaaa gccatccgtt tttccactct cgaaaaaatc tgccaagttt
    16441 tggagtgtca accaggtgat ttgttgacct ataaaaatga ctcgaatact ataccagaag
    16501 aataaaataa agtagaacaa acagtaaata aaaaataatc tgaatgatag atgtgtgaga
    16561 cttttagaag ttatttacgt ctattattca gattttttat ttcgattgaa taccgtttta
    16621 tctataaata agatatgtac tgactcatac aaagggggcc ttttcttctc catgtactat
    16681 cacttttcct gatacaactg gtttcctgtt acgttgtagt tggatctgct tttagtataa
    16741 tactttattc attccaccag actgatagca gtttgagcag tctaaataat catgtagtat
    16801 tacaggccat tctgtttcat accttgaagt gcttaaaact tcagctaatg gcccattgtg
    16861 ttaactctta ctccattctt ttttagtaag ctcccatata tactgtaaag ccttctatat
    16921 attatgtcat gttgcttttt aacatttact atgtagtttt gataaaatga tttgaataac
    16981 agtgatggac aattcttttt tatttttaac attattttga taataatgat tatttattta
    17041 aaaatacatt agataacatt tgttacgaca cgaaaaagct tcacaaacaa atatcgttat
    17101 tttaaaatga gggcgaactc acacaattag gaggaatagt atgccattta ttgtttactt
    17161 ttttctttca ttactcgtga tctatattcc attaccaaca tttttcctgc tcgtcaatcg
    17221 tctagcagag caacatgata cgattgctgc tcttacgaaa ctttgtttac ttctatttgt
    17281 ttgtctagat tactttacga gctattattg gctaaaacag acaagagtaa aaaagagata
    17341 ttatttcttc cttgtctttt tcaatctctt tgagtttctg atccattgct ttttacttca
    17401 tcagtatcat acgatccatt tgtggatcat ctgggttttc aaagtggtcg tattattttg
    17461 tatgttcacc tttcctatct caagaaaatt tagggaggca ttgttttatt aaagaagctt
    17521 ctctgtatca ataaagacac atttaaggaa aaacttttaa aaatctaatt gggaatttgc
    17581 ccgcataaat aagaggctgt gacaaaagtc ttgtcacagc ctcttattcc gtataaacag
    17641 tgttcaaaag cttgcatctt cgataatatg cccaaccaaa tgaaaaatat gagaagctat
    17701 ttttgtttgg ctgttcttat tacttgatga agaacagctt tttcacgacc tctttttaaa
    17761 agtcttttta cagttttcga gcaaagaatg atacgattag tactaaaatg atggcaccaa
    17821 tcaatgatgg cacgattgcc attcctgcaa tacttggtcc ccaagaacct aataatgctt
    17881 gaccaagtgc tgatccaact aaacctgcta cgacatttgc aataatcccc atcgaagaac
    17941 ctttattcgt gattgctcct gcaattgctc cgataattcc tccgacgata agtgaccaaa
    18001 taaatcccat gtttcgttcc tcctaatgtt ttacttcctt attactgaac ccgtggttct
    18061 gtattttctt ttactttttg tgcgccgctt ccagctgctg attttacttt atctgtttga
    18121 ttcgaagcaa attcaccggt ttttttcgct gcgtcagtta ctttgtcttg caccgtttct
    18181 tgatcttttt caaattcttc ttttgttttg atgtccacta cgttagcgtt gacttcgatc
    18241 acttctaaat gtgtcatatt ttttacttct ttgccgatta cttcttgcat ttcttcaaag
    18301 attttttcga tatctttgcc atattccaca atgacatcca tatcaactgc tacttgtttt
    18361 ttaccgactt ctgtttcgat cccagccgtt acattatctg tattcacaag ttttcctgcc
    18421 acattagaga agaaaccacc gtttgcagat aataaaccgt ctacttgttc aattgcgatt
    18481 ccgatgattt tttgcacaac tttatcttca taattcagct caccgcgcat tgaatcttcc
    18541 tgttcagaat catgctttcc ttggttattt gtatgatcag aaccaagttt tccttgtccg
    18601 tttgtgtggt cagaactaag tttttcttgt gctttcgcat ggtcagaagg atgtttttgc
    18661 tcttccacag gtttagaagg atcgattgca tcatgttgtt ctggaacttt agattctgga
    18721 gttcctggat gatgaggtgc atgcttttct gcatcatgtt tcttctcttc tactccttgt
    18781 tgaacgttag ggactactgg atctggatgt gcagatactg gtgctgtttt gttcttttca
    18841 tcgttcacac catttgattg gtttggtgtc gttgggtctg gatgtgcaga cactggtttt
    18901 tggttgttca ttttattgtt attatttttg ttattgttat tcatggatgt ttcctcctaa
    18961 gatttaaaat agtgatctaa gattttcgtt tgtttcagat aaaaaccgat agcgacgccg
    19021 atcgctgcca tgatcaagat caataaggtc ttgaaaaagc ccacggtgat ccataaaacg
    19081 gctaatatca atccaagtac cccaaagatg atagggagct tgtatgtcga aaatagttct
    19141 ttcatttttg gtccccccct attctactcg tttgcggtca ttgcttcgtt tagctggttc
    19201 ttcaaagcct gtgtatttca ccgatacttt cactggctct tttgttccca atatgtgttg
    19261 tacttcattt tcgatttctt tcatcaatgt tcctgtttta ccaatgagtg ctgaagttcg
    19321 tttcaactcc ccttttacat tgacggcgat cttgtttttc gttgctttga catttacttt
    19381 tggtgaatgg atgaattcat cttcatgcaa gtgagaacga accattcctt caattgcttt
    19441 tttatccaac gtcaactccc catcaccatg tttcagtaga aagtttccga ttgtttttgg
    19501 ataaaacagg aaaacaagta acgtgataat tgtcaaaata gcaaatgcaa cggcaagcca
    19561 aaatacatat tgttgcacgt atgaattagt gatggaaaag aatcgaaact tctctagctg
    19621 aaaaggcaat ggataggttg cacttaatag tatcgcaaac aaaagcacag atagcaaaag
    19681 taatgttccg atcactccga tcgtttttac acctttattc attttcgcac ctcctcatag
    19741 gggtgaaacg atcaacttct tataagttga tcgcttctcc accagttact ccgtaaattt
    19801 gggctgtaac ataacttgct ttattcgatg ctaagaaaac ataaactggt gctaattctc
    19861 ctggctgacc tgcacgttct agtaaacttt gctgaccaaa ttcagggatc gaatcagaag
    19921 gttgtccttc gtctagttgt aatggtgtcc agattggacc aggtgctaca ccatttacac
    19981 ggattccttt aggtgcaagc tgttttgcta aaccgatcgt aaagttagcg attgaagctt
    20041 ttgttgctgc gtaatctaat aaatgttcgc ttggattgaa tgcttgaaca gaagttgttg
    20101 ttacgattgc tcctcctgct gggatatgcg gaacagcttc tttcactaaa gcaaacatag
    20161 cgatgatgtt tatcataaat gtgtctttga cttgctcaag tggtaactca gcaatagact
    20221 gacaagaaat ttgctgtcct gcgtttagta ccatcgtatc tagcccgcca agttcactca
    20281 cggctttatc tatgatttct ttaggtgctt gttcgtcacg aagatcaaaa ggtagt
//
LOCUS       DAZULG010000048        20263 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000048 DAZULG010000000
VERSION     DAZULG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.049"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..2173)
                     /gene="glyS"
                     /locus_tag="ACG9CY_001520"
     CDS             complement(98..2173)
                     /gene="glyS"
                     /locus_tag="ACG9CY_001520"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS4938034.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2175..3092)
                     /gene="glyQ"
                     /locus_tag="ACG9CY_001521"
     CDS             complement(2175..3092)
                     /gene="glyQ"
                     /locus_tag="ACG9CY_001521"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS4938035.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3476..4288)
                     /gene="recO"
                     /locus_tag="ACG9CY_001522"
     CDS             complement(3476..4288)
                     /gene="recO"
                     /locus_tag="ACG9CY_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS4938036.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4431..5330)
                     /gene="era"
                     /locus_tag="ACG9CY_001523"
     CDS             complement(4431..5330)
                     /gene="era"
                     /locus_tag="ACG9CY_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS4938037.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5345..5740)
                     /locus_tag="ACG9CY_001524"
     CDS             complement(5345..5740)
                     /locus_tag="ACG9CY_001524"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4938038.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5724..6200)
                     /gene="ybeY"
                     /locus_tag="ACG9CY_001525"
     CDS             complement(5724..6200)
                     /gene="ybeY"
                     /locus_tag="ACG9CY_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS4938039.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6217..8409)
                     /locus_tag="ACG9CY_001526"
     CDS             complement(6217..8409)
                     /locus_tag="ACG9CY_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS4938040.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8433..9404)
                     /locus_tag="ACG9CY_001527"
     CDS             complement(8433..9404)
                     /locus_tag="ACG9CY_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS4938041.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10110..11489
                     /gene="brnQ"
                     /locus_tag="ACG9CY_001528"
     CDS             10110..11489
                     /gene="brnQ"
                     /locus_tag="ACG9CY_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4938042.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11645..12091)
                     /locus_tag="ACG9CY_001529"
     CDS             complement(11645..12091)
                     /locus_tag="ACG9CY_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS4938043.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12118..12294)
                     /gene="rpsU"
                     /locus_tag="ACG9CY_001530"
     CDS             complement(12118..12294)
                     /gene="rpsU"
                     /locus_tag="ACG9CY_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS4938044.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12456..12884)
                     /locus_tag="ACG9CY_001531"
     CDS             complement(12456..12884)
                     /locus_tag="ACG9CY_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4938045.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12976..13836
                     /locus_tag="ACG9CY_001532"
     CDS             12976..13836
                     /locus_tag="ACG9CY_001532"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS4938046.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13920..14270)
                     /locus_tag="ACG9CY_001533"
     CDS             complement(13920..14270)
                     /locus_tag="ACG9CY_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4938047.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14286..16013)
                     /locus_tag="ACG9CY_001534"
     CDS             complement(14286..16013)
                     /locus_tag="ACG9CY_001534"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS4938048.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16226..17152)
                     /gene="trxB"
                     /locus_tag="ACG9CY_001535"
     CDS             complement(16226..17152)
                     /gene="trxB"
                     /locus_tag="ACG9CY_001535"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS4938049.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17297..18730)
                     /locus_tag="ACG9CY_001536"
     CDS             complement(17297..18730)
                     /locus_tag="ACG9CY_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4938050.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18730..20133)
                     /locus_tag="ACG9CY_001537"
     CDS             complement(18730..20133)
                     /locus_tag="ACG9CY_001537"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4938051.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
BASE COUNT         5463 a         4402 c         3619 g         6779 t
ORIGIN      
        1 aacattgaat taataatagt tatttatatt tataacaaat aaaccagaga aatagcctat
       61 tttgtgcctg ttctctggtt tatttacata ttagaaatta ttttgtgatg atttgatcta
      121 aacttgctag tgacaaagcc atggcagcaa tttgacttaa ttgcgttaat cggttcttac
      181 gtactgctgg ttcttctgcc atgaccatcg tctgatcaaa ataattttcg atcagtgggc
      241 gaaggcttac aagctgatca taattttcgt tgatcgtttg gtcagcaaag cttgttttca
      301 ttgcctctac tgctttgtgc agttctttct ccgcttcgtt ttcgaacagt tcagggtcta
      361 cttctgattg tttttcaagc tccgcttttt tcgctaagtt cacgacacgt gtcaaagctt
      421 ccatcgatgg tttgaagtct tcatctgcaa gatgctgctt gaacaattgt gctgctgcaa
      481 atacttttgt cagatctttt tgttctgcat tcaaaacagc ttcgatgaca tcatgacgga
      541 tattcttcgt tgtcaataat tggcgcagac ggcctttgat aaagtcgatc acctgttgtt
      601 gtcccgctga caagccaatg ccaaaacgat ccgtatcttt actgataact tcactaatcg
      661 tttcttgcag aacagacaaa ggaaaggccc attttttcga ttcgatgatt cgaacgacac
      721 catatgcttg acggcgcaaa gcatatggat cgtttgagcc acttgggatc atcccaactg
      781 caaagaaaga aaagatacta tctaatttat cagcaatcgc taaaacagaa cctactgtag
      841 tctcaggtaa atctccttca ctggatatag gcatgtaatg ttcacggatc gctgttgcta
      901 cggctggttt ctcgcctttc agcagagcat atttttctcc catgatccct tgtagttcag
      961 ggaattctcc taccatgttg gttactagat caaatttata gatttcagca gcgcgttgca
     1021 gatccatcaa ttcttcctca gatagtccta cttgttcgcc tatgatgcga gcgatgactt
     1081 gtacccgttg cattttctca tagatgctac cgattttttc atggaaagta acagttttta
     1141 atttttcaac gtattggtcg attgaagctt tcttgtcttc attataaaag aattccgcat
     1201 cttctaaacg agccgtcaat actttttgat tgcctcgtac cacattttcc aattttaccc
     1261 gatcgccatt acgagcagaa ataaaatgag gaagcaacat accttgatcg ttccgtactt
     1321 cgaaataacg ttgatgttct ttcatagaag tgactagtac ttcttcagga acagatagat
     1381 atttttcttc gaagtcgcca gaaaagacag ttggatattc gactaaattg ttgacttctt
     1441 ctagtaaatc ttcatctaga tcaatgatcc agtgattttt ttcggctaat tcgtttgctt
     1501 gtgtgatgat catttctttt cttttttgcg gtgaagcaat aacaaattgt tcattcaatt
     1561 tttcttcgta ttcttttgca tgcgtaaatg tcacatcttc gcctaagaaa cgatgacctc
     1621 ttgaagctct gtctgttttg acatccaaga tctcaaacgg gattatttcg tcatctaaca
     1681 atgccacgat ccagtgaata gggcgaatat attcaaaatc gtattctgcc cagtgcatcg
     1741 ttactgggaa agtgaggctt ttaatgacat ctgctaaacc tgtcaatact tcttttgctt
     1801 ctttgccgtg agtgtatttc gttacataga catattccac gccttttaat tctctaaaag
     1861 tgatagcatc tgtcgtcaga ccttgtccgc ggacaaatcc ttcagctgct ttcgtccagt
     1921 ttccatctgc gtccaaagca atttttttgg ctggtccttt gacttcttct tcgctgtctg
     1981 cttgacgttc tggaatgttt gttactttga ttgctaagcg gcgcggtgta gaaaacgttt
     2041 cgatcgtatc ataagtcaat tggttttcat ctaaaaattt tgctgttttt tcttctaatt
     2101 gtttcatgct tggtgtgaca acatgagcag gcatttcttc cagcccgatt tcaagtaata
     2161 agtcttttgc catcttattc agcctccttc atgttttctg gatttgctgt gtttttattc
     2221 aataaaggga atcctaattt ttcgcgttct gccacaaaga ttttggctac agcacgagcc
     2281 atgttgcgga tacgggaaag ataacctgct cgttctgtta cggatactgc accgcgagca
     2341 tctaataaat tgaatgtatg gctgcatttc aaaatataat catacgctgg atgaacaaga
     2401 ttttcatcga tacagcgttt agcttctttt tcaaaacggt caaagttgct tagtagcaag
     2461 tcttggtcac ttacttcaaa agaatatttg gaatgttcat attctggttg tttgaagatc
     2521 tcgccatatt tcacaccatc agcccattcc agatcataga cactttcaac ttcttgaata
     2581 tatgaagcaa gacgttctag tccataagtg atctcagaag taactggttt acaaggtagt
     2641 cctccgactt gctggaagta cgtgaattgt gtgatctcca tcccatcaag ccatacttcc
     2701 caacccacac cggcacaccc catcgatggg ttctcccagt tgtcttcaac aaaacgaata
     2761 tcgtgttcta atggatcaat tcctaaaaga cgcaagcttt ctaaataaag ctcttggata
     2821 ttttcaggtg aaggtttcat tacgacttga aattgatgat gctgatacaa acggtttggg
     2881 ttttctccat aacgcccgtc agctggtcgg cgggatggct ctacataagc agcattccat
     2941 ggttccggtc cgattgcccg taagaatgta tatgggctca tcgtacctgc tcctttttct
     3001 gtgtcataag cttgcatcag catgcagccg tttgatgacc aaaatttttg taatgttaaa
     3061 atcatatctt ggacggtcaa ttttttactc atctgttttg cccctttcaa tttttaagcg
     3121 ttgctgtcaa taagaataca ggaaagaaaa cgacaaaaag tccctatgtc tgattacaga
     3181 catagggacg aataatttcg cggttccacc ctacttccga ataaagattc ggcagcttca
     3241 tttatcgtac tcgcaagtgc ctcttcaaaa atcgtttggg tccggcttcc actctcccga
     3301 actcgctaaa ccacgtgttt tttactgatc ttggtcaatg tacgtattca ttttgcagtt
     3361 ttcttgttta tataatagat cctctcagtg aacgatctat ttaatctacg gttaataata
     3421 gacggaagtc cctgtatttg tcaaatagtt ttgatttttt tgttgtatct ttcatttatt
     3481 cttctttttc aggtatctct ttttcaggca tatttttctc tggcgtcttt aatacgtgtg
     3541 cccaagactt catttgatca atgaactttt tgctttttag atgaataccg acatattcat
     3601 catacaactg gtcaatgagg tcacggattg cctgcttcgt ttcttctttc aagtggattc
     3661 cactgatctt atcataagaa atagccgaaa acattcgaac gaaataaaca gcgcgaggat
     3721 ctgcatgata acggtgttcg tccattggcc agtgtttctc gcatatcaca ccgccagatt
     3781 tggaagaata gtcaaatttt ccttttgttt ccccgcatac gacacagtgc gtccaaattg
     3841 gcgcgattcc gaagcgttgc agtatttgaa cttcaaagat attcgtgatg atttctgcat
     3901 catctccttg atccatccgc tgcagagctt gttgcgtaaa ttggaataaa tgcgggtcat
     3961 attgatgatc ttcgattgct gcatctacta gatttagcaa ataggtacca taaccgctca
     4021 aaaagatatc ttcttggatg ttccgaaacg catggacgtc tttgacactg cttaaaaacg
     4081 acaaaccttc acttcgaaaa ttaccgatat agactgcttc ggtaaagggg agcaaagccg
     4141 gagctaatgg ctgattttgc cgatggattc ctttgatata aaacatcatt ttgccagaag
     4201 attccgtaaa gattttgact aatttatctt tttctttgta attacggcta gataaaatca
     4261 agcctttcga ttctgcaagc tggctcatat tctccctcct aaaaaataaa tctggaaaag
     4321 cagattctgc cgatccagat tatttatgtt tcgatcttcc gccgtttttc tcaacgtgcg
     4381 gatttttatc cagtttattt ctatacttaa cctataagaa gttcagaata tcagtattcg
     4441 tcttttcggt agccaaagtc ttgcagatag acttttttgt ctcgccagtc tttttggact
     4501 ttgacccaaa gttctagaaa tactttgtct cctaaaagat tttcgatatc ctttcttgct
     4561 ttcgttccta cttgcttcag catttttccg cctttgccga taatgattcc tttttggcta
     4621 tctctttcga cgatgatcgt ggcctggata tggattttat cattctcgtt tcgcttcatc
     4681 gaatcaacaa cgactgcgac agaatgaggc acttcgtctc gtgttaaaaa caagactttc
     4741 tcacggatca attcagaaac gataaagtat tcaggatgat cagtcacttg atcatctgga
     4801 aaatattgtg ggccctctgg catttcactg actaatacat ccattaagcg ttcgacattg
     4861 tttccttctg tagcagagat cggaatcact tgtgcaaaag gcatctgcgt cgaataatct
     4921 tcgatgattc ccagcaactc gtctggatgg atcttatcga ttttattgat aatcagataa
     4981 actggagaat gcacgttttt tagacgttca ataataaaat cgtctccccg gccgcgtttt
     5041 tgatcagcac tgatcataaa taagactgca tccacttctt tcaacgcact gtaagctgct
     5101 tctaccataa aatctcctag acgatgcttt ggtttatgga ttcctggtgt atcgataaaa
     5161 atcagctgag cttctggtgt agtatatact ccttggattt tatttctagt tgtctgtgcc
     5221 ttgtcactca taatggcaat tttttgaccc acgattcggt tcaataatgt ggattttcct
     5281 acattcgggc ggccgacgat tgcgacaaac cctgatttat gttctggcat atacgttctc
     5341 ctttttaatt aaacaacaaa tgatatattt tagggacaaa taaaatcaac ccaatgatga
     5401 cagcaaaaca agcggtcact aaaactgctc cagctgccat atctttgatt tttttcccaa
     5461 tcggatgaaa atgaaaatcc gtaaacatgt ccactacatt ttcaaaaaca gtattgatga
     5521 tctctactat ccaaacgaga aaaacagcta aaagcagcca taaccactct atccgatcaa
     5581 gctggaaaat cacgcccata aataaagcaa ggataccgaa cgcaacatgt ttcctcatat
     5641 tgcgttcttc ctcaaaaacg gttttgactc cttggacagc aaattccaca gacatcagga
     5701 aatgtttgtt tttctctacg tttttatctt ttgagtccat aggcatccaa gatctctttc
     5761 tgtaaaccaa acatttcttt ctcatcttct ggtgtcatat ggtcataccc gttgatatgc
     5821 aagaatccat gtaccgctaa aaagccaagt tcccgatcat atgaatggcc gtaagcttct
     5881 gcttgttcag cagcacgttc aatagaaatc atgatgtctc ctagtacacg aggcaaagga
     5941 ttctctccat cttcaaaaat cactgggatt tcgtcttcgc cttcttcttc cagagcaaaa
     6001 ctgatgacgt ctgtcggtgc atcttttcca cggtaatcac gattgattac ttggattgcc
     6061 gcattgtcca tgaaagtaac agacatttct gtttcctctg gcagctttaa ataagatgct
     6121 gcaaattgca gcaagtcatc gatttctttc attttctctt ctgagacttg gtttgtttca
     6181 tccataaagg taatatccat tatacatcgc ttctttctat tcttcttgtt cttcctttat
     6241 cttctcaact tctgttttca ttttaggata tttgatccga gaatggaagg tgctacttaa
     6301 accgctgatc aatgattttt ccacttttcg gatttctttc aaggtcagtc cactatcgtc
     6361 taattgacca tcggaaatac gctcttcgat aagattatgg acaaaacttt cgattttttc
     6421 gctcgtcgga tgatccattg cccgtactgc agcctcacaa ctgtcagcaa tattgacgat
     6481 ccctgcttca cgactttgcg gttttggtcc aggataacgg aattgctctt ctgttacttc
     6541 cggattgcgt tctttcgctt tgatgtagaa ataacgcatt agcgtggttc catgatgctg
     6601 tttgcaaata tcgatcacca tttgcggcat attataatct tccaatattt tggctccatc
     6661 tgtcacatgt ccaaagataa tatgtttact gtcttctggc aataagaaat tgtgcgggtt
     6721 ttccgctcct gaaggcaagt tctcaacgaa aaagctagca tgtttgatct tgccgatatc
     6781 atgataatag caagctactc gagttagcaa agaacgtccg ccgatttctg ctacagcgtt
     6841 agcacttaga ttagctacca tcatactatg atgataagta cctggcgctt cttccagcaa
     6901 ctgtttcaat aaaggatgat ttggattgct gagttcattt aatacaatta cactgtcatc
     6961 attgaccatc aattcaatat atgggtgtag accgatcgtc agcaagaatg agaagagtgc
     7021 accagtgaac ccgcagacaa gcattaacca agtcgttgca tcaccaaagc tcattccttg
     7081 atagacgatc aaaatgacat ccatcatcac aggaaaaata atgacccaca tggcagcaga
     7141 taatccttgt tctgagatcc gtttgcgtct gacaatcgtt gccatgagcc cagagaaaac
     7201 ataagacatc aagataatcg ttagaaagtt cgtgccaatc gaatcataaa aaatgaaaag
     7261 tgcagatact gcttggaaaa taccagccat gatccctgct cttcggttca agaaaaagct
     7321 taaaacaagt ggtgcaaaag cagcgggata aaacagcggg atataagtag cttgttccgt
     7381 ttggaataat tggaaaaatt tcatcagcac tatgctgact gacatagctg tcacatagaa
     7441 aagcacgtat tttgttcttt ctcctgcatt ttggaattga agtgaattat aaatcagtac
     7501 gccgatttga agcaaaacag ctaaaaccat ggcaatgagt gggaaaatcg aagtcgtttg
     7561 attcgtcaat cctagtaatt ccaatttttt catcgctgaa gcatcaattt gatttccttc
     7621 gcgaacaatg atttctcctt ggaagatcat cactggttgt acagcttctc gtgcagattg
     7681 ttttaattct tctgtctttt tctcattcaa tacatcattg actacaatgc cttgatccac
     7741 taagtagcga atcatttgtt gctgctctgt tgtgacattc aaatactgaa tctgatcttc
     7801 tgcttcttgt ttaaaagctt ccaattcgct ttcacgaact tgtttactca tctgttcgtc
     7861 aatgagcttc aaactttcat ccctaaccgt gtgaatttcc gtttcagtca tttcaaatat
     7921 cgtcgaataa aaattgttcg gcagtttttg gtaaaacgca acgttttctg cattcacgtt
     7981 ttcaaaattt ttcttcagtg tagcaatccg ctcttctgta gtaggtttcg ggatattttc
     8041 atcttccttc gccttatcta ccttgtcatt ataagatttg ttgatggtat cattcgtctt
     8101 atcgatcaat tcgaataatt gcttgatccg attgttctgg tcatctgcca gatctttttg
     8161 ataagtatat tctggagtca ctgcttctgc tgccagtttt cttctttgtt cggtttcttc
     8221 cgtgttctcg atcgttttat tcgcacggat actctcttct gctacttggc cttctctgaa
     8281 atcattggcc ttttgtttga cactactaaa tgtaacgccg cagacaatga taaaaaacaa
     8341 gaacagaagg aaaggcaatg ctgcctttcc gagtttttct tgtagtttct gcagtgtttt
     8401 attcaacatg tgcttatcct cctaagcaaa gcttattctt gatgaagatc cgccttttca
     8461 taagctttga tgatttcggc aactacaggg tgccgaacaa cgtcacttgc ttcaaaatta
     8521 acaaaatcga tttttttgat ttgctttagt gttcgttctg catgaacaag accacttgtc
     8581 gtccctcgag gcaagtctat ctgacttgta tccccattga caatcatttt tgattgataa
     8641 ccgagacggg tcaagaacat tttcatctga gcgactgtcg tgttttgtgc ttcgtccaag
     8701 ataacgaatg catcatccaa tgtccgcccc cgcatgtaag ctaatggtgc gatttcgatt
     8761 acgcctcgtt ccatcaatcg gttcgtatga tccaatccaa aaatttggta taaagcatca
     8821 taaacaggcc gcagataagg atccactttt tctttcagat ccccgggtag aaagcctagg
     8881 ttttcccctg cctctacagc gggccgcgtc aaaatgattt tttgcacttc gccttttttt
     8941 agggcagcaa ttgctaatac tacggcaaga aacgttttcc ctgttccggc tggccctaca
     9001 ccaaatacga tgtcatgttt ggctaccgat tcaacgtatt tcttttgacc gctgtttttt
     9061 acccgtatag gttttccatt acggtctttg acgatctctt cctcatacat ctcaacaaaa
     9121 taatctaatg tacctttttg ccccattttc aatgccgtca cgacatctgg tgtacttacg
     9181 tggattcctc gtttgatcaa ttcctgtaat gtccgaatga ctgaggcagc caaggaaatc
     9241 tgggattgtt caccaatgat ctgaatgatt tctccacgtg tatggatggt tgtttccgtt
     9301 gtttcctcga tcagttttat atgcttgtcg tgtgcaccta aaagcatgct gacatcatca
     9361 gcagctgtca atctaatctc taaagaacta gactcttccg tcaaaaatag acatcccctt
     9421 tttttcgatt cattctttcc tattttacca tactcagaag aaactatata gcaaataaga
     9481 aaaggttgta aaagagtcgt ttaaccatac gtaccaagaa acgttaaaaa atagctgttt
     9541 ccattcgcta aaggtctcgt tttgataaaa agaatctgag aatgtaagtt atatgaaagt
     9601 ggagaacaaa atattccgtt tcattcagag gtattctcta ggtatttttc ttgtttcaaa
     9661 aaaagaaaac aaaatagtta aaatattctt gcatttcaaa aaaaacaggt gtattattac
     9721 gtcaacaaat aaacatacag caaagcgtcg aggaaaacag taggctattc aacgatttat
     9781 tcccagtgag tcatgtaagt gagaaatgac aataaatcga atagacgaac atcatttccg
     9841 agtcaatgtc gaaaacgtag atcattgtgg ctgcctccat tatcaggcta gcggataact
     9901 ttatcaaaaa gcgtccgtga atgaagaagc ttttttaagc gaaaaaaggt ggtaccgttc
     9961 tagaatctag agcccttttc ccaagtggaa aagggctttt ttttatattt taaggtggtg
    10021 gttttcatgg aatctgatat tagtggcatt actaattgct gcaacgttta tatcgtcaat
    10081 caaaatgata attattggag gaaaaattta tggaagaaac aaaaacttat tcatggaaac
    10141 aactattgat ggtcagttca cttatcttcg ggatgttttt cgggtcaggt aatctaatct
    10201 ttcctgtcca cttaggtcaa atggctggcg caaactgggt cagtgctggc gtaggatttg
    10261 ccatctcagg cgctcttttc ccattactag ctatcttagc tgtagtagtg acaaaaagtg
    10321 atggtctgta tgacttagct aaacctgtcg gaaaatggta tgctgccttg ttcttaatcc
    10381 ttgttcattt gacgatcgga ccatttttcg gtacaccacg gacagctgct actgcttttg
    10441 aaatggctgc taaaccattt ttgcctgaaa aatataattc attaggtatg tttttattct
    10501 ctgctctttt ctttttactt gcttatttcc ttactgtcca tcccacacgg ttggtcaaat
    10561 acgtcggaaa gtatctaaac tccttgtttc ttgcattatt agcaatcatt tttgtcgttt
    10621 ctttattcca cccaatggga aatctaaatc aatctgctgc agatacttac caaacaaacg
    10681 ctttaacaaa tggtgtccta gaaggttata acacggttga tgcagtagcc ttgcttgcct
    10741 taagtgtcac ttttgtccat gcagtccgtg accttggttt caaagataaa aaagtcacag
    10801 aattaacagc aaaagctggt agcctgagcg ttgcgctaga agtcatgatc tattttggtc
    10861 ttgttcttct cggtgcgatg agtttgaata agatgtcttt atcagaaaat ggcggaactg
    10921 cactttctca gatcactaat ttttatttag gtaacttcgg cgctagcttc ttaggagtga
    10981 tggtcacatt aggtgtcttc actaccgcta tgggactcgt tgtttctttt gcacaagatt
    11041 tccataaact gttccctaaa gtcagttata tgacatggct gcggttaacg actttcgtct
    11101 cttttgtcgt tgcaaatgct ggtttggaca atatcatcca atggtcttta ccagtattga
    11161 tgcttttgta tccattgtcg cttgcgttga ttttgctttc cttaacagca aaatttttcc
    11221 aaaagacacc atttgtctat caagtaacga tgttgtttgc tgctgttccc gccgtattgg
    11281 atatgctggc aaattcgcct gctttagtga gtcaacaaag agttgtagca agcatgctcg
    11341 aattttatca tcatcacgtt ccttttgctg cattgggact tggctggatg gtcccaacgt
    11401 tacttgggta tgctggcagc ttactcttct attacgccta tcgcctatca gggtataaac
    11461 aagaagcaaa tgaactacca gaagaataac cagatcaaac acaatttaac ataagaaata
    11521 ttaaacagga aaaaaacagc tagatgaaag aatcctattc tttcgtctag ctatttttta
    11581 gatctctttt ttacgagcac aaaaaaactg cggacaatga ttgtccgcag tttaaatgat
    11641 aaaattaaga ttgtaatagt tctttcacaa tggcatttac ttggtttcca tctgcttttc
    11701 ctttgacttt aggcatgatc gcacccatga ctttgccaaa ttctttcatt gatgaagcac
    11761 cagtttgcgt gattgcttct tgaacaagtt gtcgaatttc ttcgtcggaa agttgttccg
    11821 gcatgtattt ttcgactatc acaatttcac tttttacttt ttcagctaaa tcgtcacgtc
    11881 ctgcttcttc aaactcatgc aaagaatcac gtcgctgttt catttcgcga gacaacaccg
    11941 ttaattcttc ttcttctgtc aagtcgtgac cttctttaat ctgctcgttt tgtatagatg
    12001 acttgatcat tcggatgact tgtaaagatt ccttatcttt cgctttcatc gctgttttca
    12061 tgtcatcgtt taatctactt agtagtgaca cactaccaac tccaattcat cacagaacta
    12121 gaattttttg cgttttctag ctgcttcaga ttttttcttg cgtcttacac ttggtttttc
    12181 gtagaattca cgtttacgag attcttgtaa agtacccgct tttgacacgg aacgtttgaa
    12241 gcgacgaaga gcatcgtcaa gtgattcgtt tttgcgaacg actgtttttg acatgttaat
    12301 tccctccctc cgagcttaaa aagtaaccgt aattgttatt gaaatatatc tcaaaaaaca
    12361 agactgtcct gtaaatatta tatctaatgc tgttttgacc gtcaaggaaa tcttgctttt
    12421 ttttaaaatt atcaaatgtt attttattta aatccttatt gacaattttc acatcttccg
    12481 aaaatctcga atctatgccc ttcgatcgta catccaccga gctgttcttc gaaataattc
    12541 attggacaca tatggatttc tttcgttgtt ccgcacaccg tacagataaa gtgatggtga
    12601 tggccgattt ccccttgaca gcaatgaaaa cgaaacttca tctctccatt taaatcggtt
    12661 tcttccagta aatcgatttc tgcaaaatca tggagatttc gataaatcgt atcgtaactt
    12721 actcctttga attcctgatt catatagtca aaaacttctt tagcggatac gtagcgattg
    12781 cgcttcacta aataagttaa caatgcttct ctttttttag tatatttaaa accgttattt
    12841 ttcaatgttt ctagtgattg ttcgatcaac gtctgttttt tcatgactta ttcctccaaa
    12901 tcaaaatgat tacgaagtac acattctaga gtatggtata ataaacataa aaaaaatgca
    12961 agtgaggttt tatccatgtc ttccgattct acagaaatcg ttactttttt tgtcatctcc
    13021 gactctgctg gagaaactgc gacaaaacta gcgcaagcca ctatggccca atatcctagc
    13081 gttgagttca atttattccg cagaacattt gtgacagatg agaaaacatt gatgcaggca
    13141 ctgcaagatg cactagaaga aaaagctctt gtcctgcaca cattgatcaa tcaagaattg
    13201 atcgagatga cgcgttcttt ttgtgaagaa catcaattat ttagttttga tgtgatgact
    13261 ccgccgatat cagaaatcga gcgcttaact ggtatcaagc ccattcggca acctggcgcc
    13321 cttcatttat tgaatgagaa ctatttcaaa cggatcaaag caatggaatt tgctgtaaaa
    13381 tacgatgacg ggaaagatcc tcgtgggttt ctagaagcag atgtcgtttt gctaggcgta
    13441 tcccgaactt ccaaaacgcc tttgagcttg tttttagcta acaaaaattt aaaagttgcc
    13501 aatctacctt tgatcccaca agcacatatt cccaaacagc tttgggagat cgatcctaaa
    13561 aaaatcgtgg gactgacgaa taacccagat attttaaaca atatccgtaa agaacggatg
    13621 cgctcttacg gattaaatcc tgatactgct tattccgata tcgaaaaaat acgagctgaa
    13681 ctcgattttg cgaatgatct ttatgaaaaa ttgggctgcg tagtaatcaa tgtcgcttct
    13741 ttatctattg aagaaacagc ttcgcttatt ttgaatgctt tagacctaga agaccatagt
    13801 tattatggaa ctgaaaccac cgaagaaaca gactaacaat ttttctttaa aaaaactgga
    13861 ctgtgtcagc gaagaagatt cgttgacaca gtccagtttg tctctatttg cacctatcgt
    13921 tattgatgtt ccacatgttt gagtgtctgg ttcaaaatat ccagtacgtg agaatcatca
    13981 agactataca aaatcgcttt tccctcacga tgggacttga cgacatggtt ttctcttaat
    14041 aaacgcagtt gatgcgatac agcagattgt tccatttcca ctgcttggat gattgctgtc
    14101 acattttgtt ccccgtcttt cagatacagc agaattttga gacgagtggg gtcactcaac
    14161 attttgaata gttggctgac tttctctact tttgtttttt gcgtgtctgg caatccattc
    14221 acctccaact tgaaaaaaga ctgacttcta aagtttacca ctttttccat cagtctcaag
    14281 tttttttatt cttctgtcat cttttggata atcgtttcat aagcggctac tttttcagct
    14341 gcgtcttctg aagagtttcc ttttacaccg atataaaatt tgattttcgg ctcagttcct
    14401 gaaggacgga tagcgatcca gccattttct tccagcgtat acttcaatac gtcagatggc
    14461 ggtgtcgtta gtttttctac gtttccttca cggtctgttc tcgtcaactc tttgaagtct
    14521 tctgtttgga tgacttttgc tccggcaaat tctgttggtg cttcttcacg gaatttttcc
    14581 atcaaagcag agattttcgc agaaccttct tgtccgctca ttgtcacaga aatcgttttt
    14641 tctgcgtagt agccataggt ttcaaaaatc gtttgcaagc catcatacaa cgttttgcct
    14701 tgttttttat agtaagctgc tacttctgct aataagagca atgcttggat cgcgtctttg
    14761 tcgcgaacga aaggtttaac taaataaccg taactttctt cgaatccgaa cataaaagta
    14821 tgggaatgat cttcttcaaa ttgttgaatt ttttcagcaa tgaatttgaa tcctgtcaat
    14881 acattgaaca tggtagcccc atagtgttcc gcaattgctg taggtaattc gctagaaacg
    14941 atcgatttca ataccgctgc gttttcaggt aaagtgcctc gcgattggtg tgcttccaaa
    15001 atatattgag caaacagtgc tccgatttgg tttccggtca atacttcata ggagccattt
    15061 ggcaaacgaa cggctgcacc taaacggtct gcatcaggat ctgttgcaat cagcaaatca
    15121 gcgccttctt tctcgccaag tcgtatcgca tactcaaaag cagagtgttc ttctggattt
    15181 ggtgatttga ctgtagtgaa atctgggtct gcgacagctt gttctggaac tagtacgaat
    15241 tgttcgaatc cagcttgttt caacgcacgt tcgcccaaca tttttcctgt tccgtgaagt
    15301 ggtgtgtaca caagtttcaa atctttcccc attgtttcca ccaattcatg atcaatcgtt
    15361 actgctttga tttcttctag ataagcggca tccacttctt ctccgatgat cgtgatcaat
    15421 ccgctatgtt cagcttcttc gtcagataat acttcaactt ttaacggatt gctgacttca
    15481 cggacatatt tcgtcaatgc atctgcgtct gcaggaggca tttgtccccc atcttctccg
    15541 tagactttat atccgttgta agctgctgga ttgtgggatg cggttaccat gattcccgca
    15601 aacgtcttca aatgacggac tgcaaaagaa agctctggtg ttggacgtag actttcgaat
    15661 acataagatg ggatattgtg atgtgctaat gttttggctg cttccatggc aaattctggt
    15721 gattgatggc gtgagtcata agcgatagcg acacctcggc gttttgtttc ttcgccttgt
    15781 gtatccatga aacgtgcaag tccttctgtt gcttgtctta ccgtatagat gttcattcga
    15841 ttgatcccag gccctaagat tccccgcatg cctgcagtac cgaattccaa cggtgcataa
    15901 aaagcatctt caagtttttc aggatcttca cccagatcat ttaattgttt ttttaagttt
    15961 tcttctaaat tttcttcatg tacccattgt tcatacactt gttcccaaga catgaaaata
    16021 acacctcttt ctgaaatatg gttcttaata agtatagcat tcacttatta gatatgaaag
    16081 tatgaggacc aaaaaacgct taaatcaaga aaggattctt ttatcaagca cggttgcaag
    16141 tattcgcata aatcggattc agtagatttc acgcgaaaaa aaagagtaaa agaacaatta
    16201 aaccgttctt ccactctttt taaacttatg cagttgtttc ttctaaagat tccaagtatt
    16261 tatacacttg ttgtccagcg atcccaccat caccaacagc agtagtgatt tgtcgcaaat
    16321 ctttttcacg aacatctccg atggcaaaaa cgcctggtat attcgtcttc atttcgttgt
    16381 ctgttggaat ccagccagct tcgttcgtca accctgcttc tctaaatggt gcagttaatg
    16441 gatccagtcc aacatagatg aatactccgt ctgctgccaa atcaaactca ttgccggttt
    16501 tgacattacg tgctttcaca cctgtcacta ccatttcatt cccaacgatt tcgtcaacga
    16561 ctgtatccca cataaaggaa attttttcat tcgcaaatgc acggtcttgg atgatctttt
    16621 gtgcacgtaa ttcatctcta cggtggacga tgactacttc agaagcaaat tgtgtcaaat
    16681 agatggcttc ttctactgct gagtctcctc ctccgatcac tactagacgt ttgttacgga
    16741 aaaacgcacc atcacaaact gcacagtaag aaacgccacg tccagcaaat tcttcctcgc
    16801 caggtacgcc taatttacgg tggatacagc ctgttgcgat aatgactgtt tttccttcgt
    16861 aagttttatc ttcacaaata acttctttgt ggctgccgtg atccttgata tcttgcacga
    16921 ttccataagc attttctgtc ccaaatcttg aaacaccttc atacatctta taagctagat
    16981 caggacccat tattgaatcg aaaccaggat agttttcgat ttctgccgta ttgttcattt
    17041 ggccgccagg ggcgccttgt tcgatcatca atacggataa gtttgaacgt gaagcatata
    17101 aagcagcagt catcccagca ggacctgcgc caataacgat tacatcatac atatgtagat
    17161 tctcctttac ttaatcaagt aagcatactt tacagccaat gcgaaaaaaa gtctattgtt
    17221 ctgcttgcgt ttctcgaata gctttattat actcgaaaaa tccgctgaaa aacaatagac
    17281 ttttataaat cattgatcaa tcattcgaaa gattttctcc atttgaatca attacttttt
    17341 tataccagtt gaatgatttc ttcttcgaac gggctaatgt gccatttcct tcgttgtcac
    17401 gatcgacata aatgaatccg taccttttct tcatctcacc tgtaccagct gatactaaat
    17461 caatacatcc ccaagtcgta tagcctaata gatcgactcc atctaattct actgcatctt
    17521 tcattgcctg gatatgtgct gccagatagt cgattcgata gtcatcttcc acataaccat
    17581 tctcatcggg cgtatcgact gcacctaagc cgttttctac aataaatagt ggtttttgat
    17641 aacgatcata aatatcattc atcgttattc tcaagcctaa tggatcaatt tgccaacccc
    17701 attcacttgc ttccagatat ggatttttga cagaagcaaa gatatttcct gcagtctgtt
    17761 ccaagagttc gttgtcagta gtcgaaactc gagaagaata ataagaaaag gagataaaat
    17821 caacagtatg ttctttcagc aatgcggcat ccccaggttc cattttgatc tgaatgccat
    17881 ttcgttccat ttctttcaat gcataagcag gatattctcc tcgtgactgg acatcaatga
    17941 agaaataatt ttctcgatct gattttctag aagcccaaac atcttcagga ttgcaagtgt
    18001 atggataata tgctcctgct gccagcatac agcctacttt gttctccgga tctacttcat
    18061 gagcaatctt cgttgcgatg gcacttgcaa ccaactcatg atgtgccgct tgatatttga
    18121 tttgttcttc attttctcct tcttcaaaat acagccctgc gcccatgaat ggtgcatgaa
    18181 ggatcatatt gatctcattg aatgtcaacc aatatttgac aagtccttta tagcgattga
    18241 agatgactcg gcacagattt tcataaaaac caacgagttc tctggaacgc cacgcaccat
    18301 attctttgat caaatgcatc ggacaatcaa aatgtgtaat cgtcacaagc ggctcgatcc
    18361 cgtacttttg acattcttta aagacatcct cataaaattt caaaccagat tcatttggct
    18421 ctttttcatc gcccattgga aaaatacgac tccaagcaat ggacatccga taagtcttga
    18481 accccatttc tgcaaataaa gcaatatctt ccttataatg atgatacata tcgatcgcaa
    18541 tttttgctgg ataaaaatga ttttcatcaa attcaaacat tttctttttt ccagtaatga
    18601 caggaaaacg gtcttctccg attggcacaa catctacgtt cgctaaccca cgtccgcctt
    18661 catcataacc gccctcgcat tgattggctg ctgtagctcc gccccataaa aaatcttttc
    18721 tgaatcccat catacttcct ccattacatg tgtattgctt gataattctc ttgccagttc
    18781 atcttggact tcgattcctt ctgcttttaa ccggctgatt gtttccttga attgcggtaa
    18841 atactgctga tgtgcaataa acaactcatt catgatccgt tttgctgtct ttcctgatcg
    18901 tataagcgga ttgatcgtaa acgcttgtaa agctgttcca taatcaccag tcacagctgc
    18961 ttcgatcgtc agcaattcca tattcttcat tacttgcaac cagcctcttt cagcagcagg
    19021 aagggaacca aacgctacat ttcgtgcgcc ttgtgcgcca acataggcag tgacttctac
    19081 tacacaatct gccggcagat caggaacagc tccatcattc tttgtagata ccacgatctg
    19141 tgtttcttta tttccataga tcgaagcaat cgtttcacag gcagcatctg aataataagc
    19201 accacctcgt ttttgcaatt gttcaggttt atgatccaat tctggatctt tgtataattc
    19261 aaacagctct gcttctgttt ctttcacttg ctgcgctctt gtaccaatcg tttgatattc
    19321 ttctaaagca tgttccagca tttcttcttc acggtagtaa tagcggtggt agccacaagg
    19381 gatcatgttc atttgtttta attgttcttt gaaaaacggg atatcaaaga tatttttcgg
    19441 caatccattg tcttcatcgt ataaacgatc aatcaattct gccgtgcggt catttccttt
    19501 attatctgct actttgtgcc aatggaagtg atttagaccg gcaaacttgt agatcagctc
    19561 ttcttttttc ttgccaagca ttgttggttc tgtcatcatc gccattactg gcacattgca
    19621 taatcctatc actttatccc atttgccata tctgataact gcttctgtca ccatcccgct
    19681 tgggttcgcg aaattgatca gccaagcatt tggacataaa cgcttcatgt cttcaacgat
    19741 tgccaaaacc actggaacgg tacgaaaagc tttgaatatc ccgccagcac catttgtttc
    19801 ttgcccaatc atcccataat aactagggat ccgctcgtct ttgattcgtg catctaattg
    19861 accgacacga aactgagtcg taacaaaatc cgcatctttc aatgcttctt cccgatccag
    19921 agttgcatag attttgactt catacggaga agcatcccac atacgctgag acatctgact
    19981 gacgatctca agcttttctt ttccttcttc tacatctacg agccagattt ctcgtatcgg
    20041 taattcctca tatcttttga taaatccttc catcaactcg ggggtgtaac tgctgcctcc
    20101 accaatcgta gcgattttga ttccttttgc catgttctca ctcctttact ttttacactt
    20161 ttattatctt ttttgtaaac aaaacaaaca atagaaattc gtttctttcc agtaacattt
    20221 acaaaagtta gtgttttcta ctggattttt gtaaatatct tgt
//
LOCUS       DAZULG010000049        19789 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000049 DAZULG010000000
VERSION     DAZULG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.050"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1053
                     /locus_tag="ACG9CY_001538"
     CDS             <1..1053
                     /locus_tag="ACG9CY_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4938052.1"
                     /translation="AKQRYHCRGCHTYFTANTYIVDRNCFIAKQVRYKILEELTEKQA
                     MTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWLPRCLLMDEFRSLKNQVGPYSFSCMD
                     GDTGKLLDILPSRKKKALVSYFMQFERRARLKVKVIVTDMNVSYASLIKECFPKAKLV
                     VDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQAKYYRQLKALSRLLIKRQDKLVYDKW
                     TKWRNFGWAYLTESEVVDRLLSISDELRIAYFYYQEILQAFHDKEADTFFKLVRTMPN
                     SVPKELHSIKKAFINYESGIRLALELPYSNAKIENLHTHIKALKRVAYGFRSFRKMKT
                     RIFLLNNLITYESKNI"
     gene            complement(1141..1644)
                     /locus_tag="ACG9CY_001539"
     CDS             complement(1141..1644)
                     /locus_tag="ACG9CY_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4938053.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(1657..1703)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2241..3206
                     /locus_tag="ACG9CY_001540"
     CDS             2241..3206
                     /locus_tag="ACG9CY_001540"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4938054.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(3305..4477)
                     /locus_tag="ACG9CY_001541"
     CDS             complement(3305..4477)
                     /locus_tag="ACG9CY_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4938055.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(4464..5609)
                     /locus_tag="ACG9CY_001542"
     CDS             complement(4464..5609)
                     /locus_tag="ACG9CY_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4938056.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(5623..6547)
                     /locus_tag="ACG9CY_001543"
                     /pseudo
     CDS             complement(5623..6547)
                     /locus_tag="ACG9CY_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            6664..7743
                     /locus_tag="ACG9CY_001544"
     CDS             6664..7743
                     /locus_tag="ACG9CY_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4938057.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            7740..8368
                     /locus_tag="ACG9CY_001545"
                     /pseudo
     CDS             7740..8368
                     /locus_tag="ACG9CY_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(8478..8705)
                     /locus_tag="ACG9CY_001546"
     CDS             complement(8478..8705)
                     /locus_tag="ACG9CY_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4938058.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(8808..11396)
                     /locus_tag="ACG9CY_001547"
     CDS             complement(8808..11396)
                     /locus_tag="ACG9CY_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS4938059.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(11416..12075)
                     /locus_tag="ACG9CY_001548"
     CDS             complement(11416..12075)
                     /locus_tag="ACG9CY_001548"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4938060.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(12124..12627)
                     /gene="trmL"
                     /locus_tag="ACG9CY_001549"
     CDS             complement(12124..12627)
                     /gene="trmL"
                     /locus_tag="ACG9CY_001549"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS4938061.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(12848..13714)
                     /locus_tag="ACG9CY_001550"
     CDS             complement(12848..13714)
                     /locus_tag="ACG9CY_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4938062.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            13823..14464
                     /locus_tag="ACG9CY_001551"
     CDS             13823..14464
                     /locus_tag="ACG9CY_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS4938063.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(14526..15887)
                     /gene="mgtE"
                     /locus_tag="ACG9CY_001552"
     CDS             complement(14526..15887)
                     /gene="mgtE"
                     /locus_tag="ACG9CY_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS4938064.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(15905..16804)
                     /locus_tag="ACG9CY_001553"
     CDS             complement(15905..16804)
                     /locus_tag="ACG9CY_001553"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4938065.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(16806..17603)
                     /locus_tag="ACG9CY_001554"
     CDS             complement(16806..17603)
                     /locus_tag="ACG9CY_001554"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS4938066.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(17581..18285)
                     /locus_tag="ACG9CY_001555"
     CDS             complement(17581..18285)
                     /locus_tag="ACG9CY_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS4938067.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            18416..18991
                     /locus_tag="ACG9CY_001556"
     CDS             18416..18991
                     /locus_tag="ACG9CY_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS4938068.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            19109..19786
                     /locus_tag="ACG9CY_001557"
     CDS             19109..19786
                     /locus_tag="ACG9CY_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS4938069.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         5909 a         4218 c         3536 g         6126 t
ORIGIN      
        1 gcgaaacaac gctaccactg tcgtggctgc cacacctatt ttacggcgaa tacctacatt
       61 gtcgatagaa actgctttat cgctaaacaa gtgcgctata aaattttaga agagctaact
      121 gaaaaacaag caatgacgac aattgctaag cattgcggcg tttcttggtc gaccgtttct
      181 cggacactcg cttctctgat ccccatgaca aaagtaaaaa gaaattggtt accgcgttgt
      241 ttattaatgg atgaatttcg ttcactgaaa aatcaagtcg gtccctactc cttttcttgt
      301 atggatggcg atactggaaa attactggat attcttcctt cacggaagaa aaaggctctc
      361 gtttcttatt ttatgcaatt tgaacgacgc gcacgattga aggtaaaggt catcgtgacg
      421 gacatgaatg tttcttatgc ctctctgata aaggaatgtt ttccaaaggc aaaactcgtt
      481 gtggatcgtt ttcacattgt caaacatctc attcgtaaat ttgaggatat tcgcgtacga
      541 atcatgaaaa gctttgatcg aaacgatcca acacaagcca aatattatcg acaattaaag
      601 gcgttgtctc ggcttctgat caaacgacag gacaagcttg tttatgacaa atggaccaaa
      661 tggcgcaatt ttggttgggc gtatctcacc gaaagtgaag tggtggatcg tctgctttcg
      721 atctctgacg aattacgaat cgcctatttt tactatcaag aaattcttca agcgtttcac
      781 gacaaagagg cggacacttt ctttaaactt gtaagaacga tgcccaattc cgttccaaaa
      841 gaactgcatt ctataaaaaa agcttttata aactatgaat ccggcattcg tttagccctc
      901 gaattgcctt attcaaatgc caaaatagaa aatctgcata cccacatcaa agcattgaaa
      961 cgagtcgctt atggctttcg aagctttcgg aaaatgaaga ccagaatttt cttactaaat
     1021 aatctgatta cctatgaatc aaaaaacatc taaagtagaa aaggaattct accttagatg
     1081 ttcttatctc actctatcag tcctatttga caaagagcct tcttttttcg ctttttataa
     1141 ttagacacgc ttttcaaaat caagcgcgcg atctaaataa tacatcaatg gtgctgaaac
     1201 agccatgacg ataaactcac ttaatgcaag cgtgccatag gttgtccaaa atgcttggcc
     1261 acctgttgga actaacatat aagcaatcag tcccatgctg atggtaaaga agatcgtgtt
     1321 caatactaaa cgcatcttta catttttcac tttgttttgc aataaagctg tcaggcctaa
     1381 agaaagcaat gtttggccgc caccaaaaac cacatccatg ataccaaaac caaagaaggc
     1441 attatagacg atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa agacaactaa
     1501 atgattcaaa ctttctgaaa tacgaaactg gatcggccct gaggatacag gcgcaactaa
     1561 aatagttaac gctagataaa gagccatgat gatgccattt acaacgatta cggataaccg
     1621 ttgtctgctt ttcgtttttt gcattttctc tcctcttttc tcctagtttt tttaacgcgg
     1681 gatggttgat gaaccgcggt tatgtgaaac aaacaataat tataacagac ttttcattta
     1741 tttcaatccg tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta aacgtttgct
     1801 aaaaatcgtg ggatatggta taaagtaagt gtaaggggtc ataagaattc ccagtaaagc
     1861 agacgaagta ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat aaaaaaggat
     1921 gtgggaattt gagcaacctc ttactagaga acggtctagc aagcagctga agatctaagt
     1981 ggacatgccg aaccttggaa cttctcctta cgtaagtaat ggatcatgcg cttgctaggc
     2041 ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg ctataataac
     2101 caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat tcaaaaaata
     2161 gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata tcttgtttta
     2221 ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat ccatcggcag
     2281 gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa aataagcata
     2341 ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt gctgaaactt
     2401 tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc gatactttcc
     2461 cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat actttctatc
     2521 agatggaagt agaattccca gttgcttata ttccagcagg ttctggagat gattttgctc
     2581 gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta gcagctcaat
     2641 caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag
     2701 ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca aatcattctt
     2761 ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta ttctctattc
     2821 tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga ggaaaacaat
     2881 taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt ggcggaggaa
     2941 tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac
     3001 gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa aaacaattga
     3061 aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca acaacaccgc
     3121 aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc acaataacca
     3181 caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta catctggaac
     3241 accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt tttttactat
     3301 gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta cccaaaataa
     3361 attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg aaccaaaaag
     3421 attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta ttttgaacaa
     3481 accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag tattctgctg
     3541 gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg atcccaccgc
     3601 tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg atcggcaata
     3661 aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag gtgtggatca
     3721 ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa ctaatcaaac
     3781 taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg tttcgtttac
     3841 tgatctcttt gcgattaaat gctagattga tgacgctgaa accgctaaaa atggatagaa
     3901 acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc atccgctgac
     3961 tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt gtctgttcca
     4021 tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt
     4081 tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt atcttgatcg
     4141 tcggcatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt aagataatgt
     4201 ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca tccaatgtaa
     4261 cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc gactcttctt
     4321 cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca atattttgcg
     4381 caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc gatttatttg
     4441 ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct ttccatccaa
     4501 gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt ggtataaaaa
     4561 ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa gttgacgaga
     4621 tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg ttccgacccc
     4681 atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct atccttttgc
     4741 accagattca tttttggtat caaattacca accaatgtac ctaacaaaat cgcacacaac
     4801 gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc tacttgatag
     4861 ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt aaagcttgct
     4921 gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa gcgaatacca
     4981 tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat cgtcccgtag
     5041 agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat attacgacct
     5101 gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg cgcgagatca
     5161 acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa aaattgatcc
     5221 atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag cgaaacagat
     5281 gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc attcgcttct
     5341 acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac ttgttctaag
     5401 actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat tccagtatta
     5461 atcgtttcga gattgccacc ttgatcaatc tcgccaaagg ccgccataaa catcaatcct
     5521 aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag cagtattttt
     5581 gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc tcaaatcctt
     5641 gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta tttttccgta
     5701 actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat ttagttcagt
     5761 agttgttact tggagctgac cttttttata agcgattcca tcagaagcta ttgttcgctg
     5821 acaacacgtt ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca
     5881 tatcttttag agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga
     5941 tctggtcgca aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc
     6001 cttgtcgatt cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag
     6061 ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt
     6121 tcaaccgacg tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta
     6181 atcccactaa ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat
     6241 ataaaccgca aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt
     6301 cttgaggaac gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc
     6361 cgaacaattt aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag
     6421 tcgatttccc actgccgttt ggtcctaaca gacctaagac ttctccttct tgtacttgca
     6481 gatcaaaatg attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga
     6541 taatcatcat gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag
     6601 aaaagaaact agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct
     6661 gctatgcata aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt
     6721 ttgctggtaa gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta
     6781 ttcactggtg tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta
     6841 tttcttttat gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg
     6901 atcgcccctg aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg
     6961 acggttggta atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc
     7021 ttttacttac gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc
     7081 aaagatactc attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg
     7141 tcacaagaaa cacaattagc cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac
     7201 atccatgaca atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa
     7261 atgatcaatc aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac
     7321 actgggatgg atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt
     7381 caacactcct tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac
     7441 ttgtttgaag gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc
     7501 ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg
     7561 ccggcctttt atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct
     7621 cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat
     7681 attttccaag aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa
     7741 tgataacagt agtcattatt gagatgatcc atttgtgacg aaatcgttac aaaccatttt
     7801 agaaagcacg aaagagatcc gtgtgttagg cattggtcat tgtgccaagg atgcgctagt
     7861 cttatacgaa acccatcgtc ccgatgtttt gctgacggac atccgcatgc ctaaacaaac
     7921 gggcatcgat gctgccaaag aaattttggc taaatttccc aaagccatcc ttttgctttt
     7981 gacgacattc aaagatgaag aatacatccg cgaagcattt tccatcggtg tcaaagggta
     8041 tttaatcaaa cagaacttgc aagcgattat tccttctgtc aaatccgctt ataatggaca
     8101 ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga ataaaccgac
     8161 gattgatagt catgcttatg cttcttttag tgaacgtgag cttgcaatca tcaaagaagt
     8221 agctgccggg aaaaacaata aagaaattgc agatgccttg tacttgagtg acggcactgt
     8281 tcgcaattac atcagccaat tactagaaaa gctagattta cgggatcgca cgcaattggc
     8341 tatctatttt tatcagcatt tgcaataagt cttatctaac acaaaaaaac ctgaatgatg
     8401 ctccttcgca tcattcaggt ttttttcaca tcttctattc aattgtcccg caaaccattt
     8461 cctcacgatt gaaagaatca aggattcgca gggctgccag gattcgttgg attgttcggc
     8521 gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt ctagttttga ttcttcacga
     8581 tcgtctcgtt ctacatcagg actttcattc aatttgaatt ctttttccat ctcatctaac
     8641 tttttaaagt cattgccaga ttcttttgct aatttcgcta atttatcttc ggtttcttta
     8701 gccataaagt aacgcccccc ttttgatttc agcttagcat atcacactga aaaaacaaaa
     8761 gaaaacgcta accaacgatc atttttagct atttctcatc tttaaaatca agcatttttt
     8821 gccggtataa aatcgtaggg agaactgttt tccataccaa tcatcggatc tactaactgg
     8881 ttatcgatca cttctgctgt aagaacgaaa tctttagaaa cagcaggttc tacctcttct
     8941 ttcttttctt ctccaacaaa tgaagaaaac agatctggtt ccactgtttc cattacttgg
     9001 tttttcgttt cattcgtccc actgtcatct tctacaggta gcttttcttt tacaacccgt
     9061 ggctcttttg tatagttccc atcttcaaat ggatcatttc cgaccaaaga atcttcataa
     9121 gctgctagtt tggtccggag agtgatattc attttatcat tatccatgat ccttgatttc
     9181 aacgtagtca tccggcttgc tgattcattc gctacacaag tgacataggc ttgctcttcc
     9241 cctaacaaaa tcaacagttc cttactgcga gtcactgccg tgtagagcaa attacgctga
     9301 agcatccgct gatattgatg gaccatgggc aaaataacca tcttgaattc actgccttgt
     9361 gatttatgga ttgagcaaca ataagaaaga gtgattttat tccattcgtt tcttttataa
     9421 gtgacctcat ttgcatcaaa ctggataact aattcatcca ctttatcttc agaatctttt
     9481 gccaagatga tacccgtgat ttctcccata tctccattga atacattcaa ttcgggagta
     9541 ttgactaatt ggaggacttt gtctcctatc cgataaacgg tgtcgttcca atgtacttcc
     9601 ttttttccag gtttccctgg attgaatatc tcctgcatca ttttatttaa cgcatcgatc
     9661 cctgcaggtc cccgatacat tggtgctaat acttggatat cttgtgcacg gaatccctta
     9721 tttttcgctt tttccacaat tttagagatc aagggttcaa tttgataagc atcacttcgg
     9781 aaataggaac gatctttttg attctttgtg aaatcttttg gcagacgtcc ttctttgatc
     9841 tcatgggcaa gaggaataat actagaacca tcaccttgcc gataaatctg attgagttcc
     9901 attttgggaa tttgttcgat ttccaaaaga tcatggagta cttgaccagg accgacagag
     9961 ggaagctgat ctttgtctcc tactaagata acttgcatat tcgtcggaat cgctttgaat
    10021 aatgtgttag ccagccatgt atcgaccata gacatctcat caacaatcaa aagtccgcct
    10081 tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc cagaagacga
    10141 tggatcgtac tggcaggcag acctgtcgtt tcgttcattc gtttagctgc tcggccagtt
    10201 ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt ttgaaggatc caattccagt
    10261 ccgttcaact ctgcaaaaag attgacgatt ccgttgatca cagtagtttt ccccgtacct
    10321 ggtcctcctg ttaaaataaa tagaggggaa cggattgctt cagcgatagc ttgttcttgc
    10381 gaaggtccat actgtatgcc gaatattttt tcgatacgcc gaatattctt ctgcacttct
    10441 ttttcaggat aatggatttc ttttttacga ctgagaaggc gctggatcga attaccgatc
    10501 ccccattcag aaaagtacaa actgttttcg taaagattag tctcttcttg ttgtatcttt
    10561 ccttcttcaa caagttggat aattacagta gcaacctgat caggctggat ttcaattggg
    10621 cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta
    10681 tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc
    10741 ggtgaatctg ctgcaatacc gatttgttcg gctagattat ctgcacgttt gaacccgatt
    10801 ccttctatgt cttcgactag ctggtacgga ttttcttcga tgatatccaa agactcgttc
    10861 ttataggcct ggtagatact gaaagctaac tgactaccga acccgtaacg attcagtccc
    10921 atgatcactt gatccatccc gtggtttagc cgaatcgtct cagcaagcat ttctcttttt
    10981 ggcttcgtca aaccggagat ttgttttaat accgaaggat cagcaatgat tttttcgata
    11041 gcatcttctc ccagcaattc tacaattttt tctgctgttt tcttcccgat tcctgggaat
    11101 ttttcgctgg aaagatacgc tacgatccca tttgcagttg ttggcttttc ttgctcatat
    11161 ctttcgagtt taagctgcct tccgtattta ggatgatcca ctaattctcc ataaaaacga
    11221 taagtctcat tttcttgaat atcgccgaaa cttccggtga ctacaatctc tttttcacgg
    11281 taatcagctg tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt actaggattt
    11341 tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca tttcttctgg ctgcatcgga
    11401 agccgcctcc ttcttctata gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt
    11461 ttggttccat actttcagtt catatggcaa taatctgcaa ggctcttcag tctctaaaat
    11521 cgtcaaacta ctattgaaca atccacccat ttcacgcaac tcacttagct cttttcctgt
    11581 catccattga atggctgcag ttaaagaagc tccatgtcct acgaataaaa gtgggccatc
    11641 tttatgttgg gcaaccgcat ctgctacaac agtttcgata cgtgtgattg ccgattcaat
    11701 cgtttctccg tgaaaaggag aaggatcata cagatctaaa tgattacgca aattcggcag
    11761 attatccgga tatttctggt acatttcatc gatcgactga ccttctaaag tacccagccc
    11821 tagttctctt agcttatctg tataaaaaat ctctaccgga cgagcaagct ctttgttgat
    11881 accttctgct gtatcacgtg ctcgctttgc tgtactggaa taaattgctt taaaaggaac
    11941 atattttacc tttttcccaa atgctttgat ttcttcgtaa ctttgtggca aaagcggaga
    12001 atcgccattg ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc catgtcgggt
    12061 aaaatatagt tccatacgca gcaaactcct gtctttcttt tattttactc tataataaac
    12121 tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat tctaaattag ggaatccttg
    12181 ctggcgaagt gcttcataca ctatcattgc agctgtattc gataaattca atgagcggac
    12241 atgctcatca ttcataggga tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc
    12301 tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc tcttctccat cagcaaaatt
    12361 ttcgtcacta tatactttat gggcaaattt tgagatcaaa tgtagtttac gattacctaa
    12421 aaatgttaaa aaagcttcta aattactgtg gtaagtaata tttacatcat tccaataatc
    12481 caaccctgcc cgttttagat gtttgtcatc agttgaaaaa cctagtggct cgattaaatg
    12541 aagcggagta ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg cagggattaa
    12601 tggttcaaat aaaacgatat gattcatcag gatcattcct ctcatattta tgttcgtttc
    12661 tattttctga caatttgatt tttgcataaa aaaaaacgta tcaactcggt gtcagtcact
    12721 gcgagtcgtt acgttagtga agctcttctc actaactttt atcagaacag gaacctgata
    12781 aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc tcaacttttt tttgcaacct
    12841 ctactcatta aaaactgcta gaaaaacgtt ttctcttagt tccttttaat aattctatgt
    12901 caattgtaat cttcttcaaa ggattcttat ataagtctgc tttgattcgc tcttctacct
    12961 gccattctaa aggtgacatg gccaaaagat ctttttgatt ttcctctggt atctcaaatt
    13021 cataggttac acgctgacgt tcagttaatt tcattgcttc agcaaacttg gctactacac
    13081 gttcattgga ataactctgt ttttcaggtt gattcggata aaaagcctga cgtagttcct
    13141 ttaaataatt ttcttgtggc accaccttca acacttctcc atcttctgag agtacacgtt
    13201 ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag
    13261 caaacggcaa attggtcaaa tcagctacac accaaaatgc ttcggcagat tgttctgtag
    13321 ctgcgtagac tccttctttt gaaatatcaa atccaacagc attttgaatc tttttcaatt
    13381 ttgaaagttc attcaagaag ctaccttctc cgcagccaac atctaatagc gagacttctt
    13441 caggtaaatg ctgcgcaata gtctcaataa cgggctgata catgccagat tggatcattc
    13501 tacgccttgg aacgaacatt ccttgatcgt attctgtttt aaccgcatgg gataaaaaat
    13561 aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt tttgcatatc aagccattat
    13621 cagacttttt tacttcttct tggcaaaccg gacagcggaa cagctgatga ttttctgcca
    13681 aaaactggcg tcctcggtca atttttttta acatagttcc ccctcatttc tgttttatct
    13741 taccgttaga tgctcacttt ggcaactgct aaaaatatga tacaatgcct agaagaaagt
    13801 gaaagataaa ggagaagatt agatgatcaa agaattttgc gctgaaaact acacatctat
    13861 tcctctagca attgctagag gagccgatcg gatcgaattg tgtgacaact tagcggtcgg
    13921 tggcacaacc ccgagtactg gtgtgatcga agaaacgtta agttatgctg gggaaaaaga
    13981 tatccccgta atgacgatta ttcgtcctag agggggcgac ttcgtctata acgatatcga
    14041 attgaaaatc atgcatactg atttgattga agcaaagaaa ttagggacgg atggcgtagt
    14101 atttggttgt cttactcctt caggctggct agacgaagag gccttagaac tattgatcga
    14161 aacagcagaa gggttgcaga tcaccttcca tatggctttt gacagtattc ctgaagatcg
    14221 gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt gaccgtatct tgactcatgg
    14281 cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat tgattgccta
    14341 tacagatggc cgcttaatca ttttgcctgg tggtggtgtc actgcagaga atgcagcaca
    14401 tgtagctaaa gaattgaacg taagcgaagt acacggaacc aaaatcgtgg atcttcagtt
    14461 ataatcttat aaacaaaaaa acatttgcaa caggcataat gcttattgca aatgtttttg
    14521 tttttttaca taaaatagcc cataaataaa gaagcaatat tgaaataaat cagtacactc
    14581 gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc aaatcctaga
    14641 cgatccatta gcatcgggat taaactacct gcaagattcg ccactgtgat tgcaaatagc
    14701 atagccatcc ctatgacaaa ccccaagata aaattatgtt tccaaaaacc tacgattaga
    14761 aaaatcgtga tccccgtaac agctcctgtg acaacgccgg tcaagacttc agccaaaaca
    14821 agtcgaacaa atccatgatc tttctcatca gaaatcgcaa gtcgacgaac agccacagct
    14881 agcgactggg ttccagcatt accggcagtt cctgtaatca gcgaaatgaa gactgctaaa
    14941 atactcgctt cacttaccaa gtcttcatag tgactgatta aagaagcggt cgacatacct
    15001 aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac tggattttca
    15061 ttaatttctt ccacattgac cccggcaagt cctgaataat cgctagcagc ttcatcatca
    15121 ataacgtcga tgatatcatc gactgtgacg attcctaaca aatgatcatc ataatcggta
    15181 acaggaagag ctaagaagtc ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg
    15241 cctacatgaa ctgaaagcac tcgttcgctc atcagatcag aaatcattgc gtcgtcatca
    15301 ttcacgatta ggtcgcgcag agaaataacc cctactagct gaccttcttg attaacgaca
    15361 tagatgtaat agattgtttc agctacatct gcttcgttct tcagcacgta catcgcagag
    15421 cgaacagttt gattggctac gatcgaaaca aattcagttg tcatgatcgc gccagccgtt
    15481 tcatcctcgt aatggagaag ttccttgatt tcactggcat cgtctgccga catcaaactt
    15541 aaatatttag caatttgctt tttatccagc gtattaagca agtcaacagc attatctgta
    15601 tacatctcag aaagcatatc agctgcatag ctaggacgca tttcatctag gtattctttc
    15661 atatgttcgt tgtcttcttc aatcacatcg aacatatccg ctaattcttt aggcgacaga
    15721 taggaataga tcagctgcct gttctcttct gtaagagatt ggtagaattg cccctgctca
    15781 tagatatgca tctctaaaaa actgtttctg aatgcctgaa tattattttc ccttaaatct
    15841 tgagtcaact gggcaaatct ttcctctaat tcttgatttt catccaaacc aatctcacct
    15901 cgccttattc ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa ggctgcttga
    15961 actccagcaa ctcttctgta aatggatggt aaaaacgcag atcataacaa tgaagggctt
    16021 gtctttttat tcccagatcc attcgcccgc catacatatc atcaccgagc agggcacagc
    16081 caattgcgga aaaatgaact cttatttgat gtgtccttcc tgtatgcagc tggatatcaa
    16141 ctaacgcgat ttctgttagt cgcttctcta tccagtactc agtctgtgcc gaacgtcctt
    16201 catcagagac cattcgcttc aatagcgaag aataatctcg ggcgatcggc aaatcgatat
    16261 gtccatgtgc cgataaatct gaaagccttc cactgactaa ggcctgatat tttttcacaa
    16321 acttcttttc acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa
    16381 gcatcaaacc agatgtatcc cgatctagtc gcgtcaccac atggattact tggttttcgt
    16441 aaccttgttt tttataataa gcctttacac gattagccat tgtcccattt ggatggtatt
    16501 gggcaggaat agacgaaacg cctgctggtt tatttactac cagaacgtgt tcatcttcat
    16561 acacgatatc gatcggtgtt tcatccaaaa gtacggtttc atgttctcct tcagcaggaa
    16621 tcacaatggt taccttatca tccttagcta gagaaaacaa gacattttct acttgatcat
    16681 taactttgat ttgaccgcct tgaaatttta ttttcgctaa cagacccttt gagatccctt
    16741 tttcttttaa aaaatatttt acttgctgag aatgatcttt ctcataatcc catgtaaact
    16801 gcatatcaat cctcactaat aaaggcatct ttcacacgat gccaaaaatg catatgtcga
    16861 taagaagcaa aatggatccg ttcttctgct attcgataat aaattgagcg gatatttgct
    16921 tttgcgacat tcaattgatc aaccgttaca agataatctt ctgtattttc caattttatt
    16981 tctagccatt catcatgggc aatcacgatc ggagaaccca aagtacgaaa cactcgatta
    17041 ttcagtgaag caatctctgc caactgaaag gcattgatac taggatggat cacagcaccg
    17101 ccgacacttt tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct
    17161 ctaaaacttt caaacagctc gtttttgatg tatacatcgg ctaccatcgt acgattggcc
    17221 cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc atccaaataa
    17281 ctgatccgaa catccagcaa gggataacta acactttgtt cccggttcgt tcgtaagctg
    17341 tctactagtt cttccagctc gtaatcccgc caatccgtgt aaaaccctaa atgacctgta
    17401 tgcacgccca agaaacgcac atcattcaga caatgactgt agtgatggaa cgccgacaat
    17461 aatgtgccgt ctccgccgac tgagatgacc aattctggct catgatcatc tcgttgaatc
    17521 cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt tgcttcttta
    17581 ttatgaacga tcgctacttt catcccctgc ctcctctctt tcgctttctt tcttcaaaag
    17641 cgttcgatag tagacatcat ctgattttcc tcttccatgg gagaataacc gctgtgcttc
    17701 ttggatttct tcacggatac tggacatctc ttcatccaac tgataggcag cttctgccgc
    17761 acgctgaagt cgttcactca tttcttctgg aaaaacgcct tgatatttat aattcagcga
    17821 atgctcaatc gttgcccaga aattcatcgc aagagtccga atctggattt ccgccaaaat
    17881 tttcttctca ccgctgatca gctgtacagg atactcgatc accagatgat aagaacggta
    17941 gccacttgct tttttatttg tgatgtaatc tcgttcttgg agaactttca tgtcttttct
    18001 tttacggatg atctcaacaa cttgatggat atcttctaca aactgacaca tgatccttaa
    18061 cccggcaata tcagacattt cttcttctaa acgtcccatt ggaatatgcc gtaattgtga
    18121 ttttgttaca atactatcga ctggcttgac tctcccagtg acaaattcaa tcgggacatg
    18181 tcgattttgt tcacggaact gtttgcggat cccccgcagt ttcactttca attctgatac
    18241 agtttgttca tatggtgcta aaaatgcttc ccaatctcgt tccatagctg atacctctac
    18301 ttcattaaca ttcattgttt attctaccat atatgcaaag ttttttcgta ctcttgattt
    18361 atgcattgac caaaaaaaga aaattaggca aaatagacaa aaaggagtcg atcaaatgac
    18421 acaaatgctt gaaatcgaat ataaatctat gctgacaaag caagagtacg agatattcat
    18481 cgctcactat caattaacag ataaagattt ccgtgtacag actaatattt atttcgatac
    18541 cgttgatgaa cagctgaaga aacaaaattg tggcctacgg atccgtttcg ttggtcatca
    18601 agctgaatat acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa cggatacctt
    18661 tataacagaa gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt
    18721 ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca ttaatccaaa ccggaaagct
    18781 tacaacaaaa cgagctgaat tccctattga agaaggactt ttagctattg atgaaagctg
    18841 gggagacaat ctacacgatt ttgaactgga actagaggta ggggatgcat ctgtgggaaa
    18901 aattgctttt ataaattttt taaaacgttt ttctttacct tatcgtcctg ctaagaacaa
    18961 gatccaacgt atgcttgaag ctaaaaatta gaaacctttc attttcacaa ggcacttgag
    19021 ttcatttttt tacttttttc tgttaagtta taggatgtga aagaatctag tcactagttg
    19081 tatagaatct attttggggg agattcatat gatcgaaatc tatttatttg ttaatccttt
    19141 aggggaatac tgtttcgaaa tggaacaaca gttattacaa tttatcgaag aagagtacgg
    19201 gacaccatca aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat
    19261 tggggatgtc atgcaacaaa aaggaatttc tcagaacaat cttgaggaac gtaatcactt
    19321 attctcaatg acttattctg cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa
    19381 aaaaggccgc cgttttttga tcaaattgca agaagcagtc ggctgtaatg acataccata
    19441 ttctcaagaa cttgctgaat ccatatttga agaaatcggc ggcgatttga atatgtttaa
    19501 agaagatcgt cagtctgact tcgttaaaga agtattcttt tctgatcaaa aaattgctcg
    19561 ggaaatgggc atcacaaaac acccttctgc agtcgtttac aattatgcct gcgaccgtga
    19621 ttttggtgtt ttagtagaag taaaaggtac aagtagcctt tggagaatca cagagctgtg
    19681 taaaacaaca gataactcat atcaagtttt taatggtgaa catttacata caaatgattc
    19741 tcagttccat tcaagaacta gccatttaca cttgctttct aattaaaaa
//
LOCUS       DAZULG010000050        19778 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000050 DAZULG010000000
VERSION     DAZULG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.051"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..884)
                     /locus_tag="ACG9CY_001558"
     CDS             complement(<1..884)
                     /locus_tag="ACG9CY_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS4938070.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLIDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIF"
     gene            1069..1908
                     /locus_tag="ACG9CY_001559"
     CDS             1069..1908
                     /locus_tag="ACG9CY_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4938071.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2104..3078
                     /gene="sgrA"
                     /locus_tag="ACG9CY_001560"
     CDS             2104..3078
                     /gene="sgrA"
                     /locus_tag="ACG9CY_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS4938072.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            3376..3732
                     /locus_tag="ACG9CY_001561"
     CDS             3376..3732
                     /locus_tag="ACG9CY_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938073.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            3777..4892
                     /locus_tag="ACG9CY_001562"
     CDS             3777..4892
                     /locus_tag="ACG9CY_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938074.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(4918..5358)
                     /locus_tag="ACG9CY_001563"
     CDS             complement(4918..5358)
                     /locus_tag="ACG9CY_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4938075.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5518..6024
                     /locus_tag="ACG9CY_001564"
     CDS             5518..6024
                     /locus_tag="ACG9CY_001564"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4938076.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            6192..8105
                     /locus_tag="ACG9CY_001565"
     CDS             6192..8105
                     /locus_tag="ACG9CY_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4938077.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            8110..9111
                     /locus_tag="ACG9CY_001566"
     CDS             8110..9111
                     /locus_tag="ACG9CY_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4938078.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(9313..9519)
                     /locus_tag="ACG9CY_001567"
     CDS             complement(9313..9519)
                     /locus_tag="ACG9CY_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS4938079.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(10165..11013)
                     /locus_tag="ACG9CY_001568"
     CDS             complement(10165..11013)
                     /locus_tag="ACG9CY_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4938080.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            11177..12079
                     /gene="gnd"
                     /locus_tag="ACG9CY_001569"
     CDS             11177..12079
                     /gene="gnd"
                     /locus_tag="ACG9CY_001569"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS4938081.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            12081..13082
                     /locus_tag="ACG9CY_001570"
     CDS             12081..13082
                     /locus_tag="ACG9CY_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS4938082.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFNVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMCEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            13079..13363
                     /locus_tag="ACG9CY_001571"
     CDS             13079..13363
                     /locus_tag="ACG9CY_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS4938083.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            13396..14193
                     /locus_tag="ACG9CY_001572"
     CDS             13396..14193
                     /locus_tag="ACG9CY_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4938084.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLSSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            14207..15040
                     /locus_tag="ACG9CY_001573"
     CDS             14207..15040
                     /locus_tag="ACG9CY_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4938085.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            15058..15915
                     /locus_tag="ACG9CY_001574"
     CDS             15058..15915
                     /locus_tag="ACG9CY_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4938086.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            15937..16446
                     /locus_tag="ACG9CY_001575"
     CDS             15937..16446
                     /locus_tag="ACG9CY_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4938087.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            16483..16884
                     /locus_tag="ACG9CY_001576"
     CDS             16483..16884
                     /locus_tag="ACG9CY_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938088.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            16996..17349
                     /locus_tag="ACG9CY_001577"
     CDS             16996..17349
                     /locus_tag="ACG9CY_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938089.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            17383..17826
                     /locus_tag="ACG9CY_001578"
     CDS             17383..17826
                     /locus_tag="ACG9CY_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4938090.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            17843..18928
                     /gene="uxuA"
                     /locus_tag="ACG9CY_001579"
     CDS             17843..18928
                     /gene="uxuA"
                     /locus_tag="ACG9CY_001579"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4938091.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            18921..19634
                     /locus_tag="ACG9CY_001580"
     CDS             18921..19634
                     /locus_tag="ACG9CY_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4938092.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         6756 a         3046 c         4145 g         5831 t
ORIGIN      
        1 ttgaatatat tttttacata aggatcttta atagttacat tgtctttttt catcacttta
       61 cggattgttt tatccatgaa ttccgtatca agagtttttg cttcggtagc aattttttct
      121 agctcagatt tgtatttgtc tttattagaa cctttatcct gtttttttac catttttact
      181 agataatagc tttgctgata agtcgttgga tctgttacag gaatgatatc agaaacttcc
      241 ccgtttttta gtttaaatgc tgcttttttt acttcagtag gaatctctgg attggttgaa
      301 tcaaaattca tttttccatc agtttccttt aatgttttat tttttccata agcttgaaca
      361 agcttggaga aattttctcc ttcatttgct tctttttttg ctttatctgc ttcttcttta
      421 gtcgaaaata gaatgatctg tgtactaact tctggataga attctttcca agcagtgtct
      481 agatctttat ctattaattt tacattggct ttcaaaccgt attggaaagc cagcatctgt
      541 ttaaagagtt tcttatatgt ctcttctgtt tggccaactg cttttaattg ttccttgtag
      601 tttggaccga gttttttgat ttgttcatta tattcttttg ttacttcctt tgaagaaatc
      661 tcttttccgt atacatcatc aaatgctttg aagacaatca ttttttttaa tagtgtttga
      721 ttatttgatt taacttttgg gttatcataa aaatcctgaa ctgtaagtgt tccaccttta
      781 aacgttgcaa tcgtttcatt ggacttgcta caacctgaga atagtaaaat tgcacatgct
      841 gaaaggcaca taattagtag ttgttttttc aaaaaatttc ccatcctttc cttattaaaa
      901 acagtctaac agaagaatta gggagacagt ctttcatttt cgtaaggttg ctgtagatat
      961 tcttagttcg taaaaatcta ttctctctat gatggagaac atttacatag ttttcacgag
     1021 acaagaaaga aaagggttgt taaacttaat ataaaggagg gatgaaagat gatcaggatt
     1081 attcggttga ttggatctgt tttaatagtc ttagttggtt ttgttttagg aattcttgtc
     1141 aataatgcac ttgcggatat gtttattgga aatagtgaat ggattggagc aattgccggt
     1201 acggtcggac atttagctgt ttttttacta gctttatttc ttgctagtaa ggtcgaagga
     1261 aaaagaatag aagattatgg tgtttctgtt cgaggaaaag actgggggta tttagcggga
     1321 ggtctcgttg ttggaattgg agtgtttctg cttattacta gtccattata tttgacgggg
     1381 gcgtatcggt tagattcagg aaacgcaaac attgtgccgc tgataacgag ttttatttta
     1441 tttattgcag tgggggcatc agaagagctt ttattccgtg gcttttttca gcatcagatg
     1501 cttagatttg gtccgttaat agcaatgatt ggatctgctg cattatttgc tcttttgcac
     1561 ggattgaatc ctaacatgac atttctggca gttttcaata tctttttagc aggttgcttt
     1621 ttcagcgcac ttatctacag tacaggaagt ttatttacgg ctattggtgc tcatattact
     1681 tggaattgga cacaaggagc tattttgggt ataccagtca gcggaacgca agaacaaggt
     1741 tttttctcca ctctgatcca atcgcaaaat acaataatta ctgggggaaa ctttggggct
     1801 gaagcatcta tatcaactac attattcttg ttagtcttga ttgttggttt gttactctat
     1861 gcatataaaa caaagcggat gaagaaatac actgctgttt atcaataaaa gatgaagaaa
     1921 gcaaggctga tatcggtctt gcttttttaa gtctgtcatt gattaaacga aaaagtaaac
     1981 aaattcaagc gaatacacga catcttttgt tagattttca caactgactg tcgtgttctc
     2041 atcattttca ttttatataa ttcctttatc aaatagaagg taattatatg gagagtgata
     2101 gaaatgaaga aaacagcgac cgtattattt gttattcttg ggctgagtat aattgcagca
     2161 atgattggaa tcagtacttc tgcaaatgaa cgggcaaatg agaatctttc atttactgtg
     2221 aaaactgatc ggattgttta tgacatgaca caacaagtca ttactattcc agtcaaacca
     2281 aacaaaagcg tgaatgctag tgatgtacat gctgttctaa catatggttg ggatggtaat
     2341 ggaagttcag aaaaagttat tggggaagtt tatttaaaag acgtacaatg gaccgctggt
     2401 attgagtata caataatgat ttctgcagaa ctatcgattg atgagatcaa gagtaaagat
     2461 aaagttgatt tgattgtttt ttatgatgga cagatgacga tcacggagaa tttgaaacca
     2521 agcagttgga cagttgttgg accgccaagc agtacggata gcacaagtag tgaaagcagt
     2581 acagaaaaca cgagcggtga aagcagtaca gagagtacga gcagtgaaag tagcacagaa
     2641 aacacgagca gcgaaagcag tacggagagt acgagtagtg aaagtagcac aggaagcacg
     2701 agcagcgaaa gcagtacgga gagtacgagt agtgaaagta gcacaggaag catgagtagt
     2761 gagagtagca caggaagcac gagcagcgaa agcagcacag gaaaagtaaa taatgaactt
     2821 gatttggtac ctatcatacc aactaaggaa caaaagagaa atgcagggag caacctaaaa
     2881 agtgtgctaa aacagcctga ttttgtttct cgtggaattg atcatcaaga aaaagcgaaa
     2941 gaaacagata atagaaaaag cgatttacca gaaactggta gtagaacctt gaacaagcta
     3001 attacatgga tgggtgtgtt actcattttg attgtaggag caagctattt tcgttctctc
     3061 ttccatcgag tgaaataaag aataaaagca acagatctaa aattgaataa aacgatagat
     3121 ttttgagtga atatttcatt cgagagtgct gtattgcctg gcgaagttgt agctttgcca
     3181 gatctatttt tgtttactaa catatttgag aaacaggaaa taacaggaac acaaatggaa
     3241 aataagagca agaataattg acaatgcaca ctgcgcatgg tagtatctct gaaaaatcag
     3301 taacgaatct atttgtacgt tattcatgta gctgtcggaa taagtctaaa taatagataa
     3361 aaaggagtat gtactttgtt tgaagaattt attgatatca atgaaaggca agtatatcaa
     3421 tttttgaact attgttatga aagagacgag aaattatatg tggtgaagga tatcgcttta
     3481 gatctaaact acaccctggc taagatgaat tcggtgattc aacaagcaga atcgttttgc
     3541 gaaaggtatc cagaatataa attatctttt ctttcagaaa acaagatgat aaaagtcgag
     3601 ttttcttctc agtttttact gagtaaagtt tattctatac ttttagaggg aacgattggt
     3661 tatattttac tggattcact ttacaaaggt acttatcaat ccttagaaaa tcttagtcaa
     3721 aaaattatat aagtttgcgt acgattcaaa gaaaattaag agaacttaat actattttga
     3781 aaaactatca tttatcttgc agcttgaaaa aaggtaatcc gatagaagga gaagaatacc
     3841 gtatccgata cttttttcac ttgatgtatt ggcagtcatt cgacgaaaat cattctgata
     3901 tattatcaag tataaaaaat aaacgtcaac tattagaaaa atttgcgaaa catgcgccat
     3961 atacgcgtag tatagataga aagaaatgga tcagcctact aagaatttcg ttgcaacgaa
     4021 tgaaacaagg ccactcgatt acgtatgtcc cagaagagat gaacaaattt gtgcatccat
     4081 tgataacaaa agaagagttt acgcattaca tactagagcc tttttttgaa gcaaacttct
     4141 ggtcgatgaa tcacatcact ccaacagagc aagcttacct ttattttatg tttggtgtaa
     4201 tgaacaccta tttggaagaa gatttgttgg atactccaga taaagtacaa gaaagttggg
     4261 gctggattga atcgtctatt gtagaaaatt ttgaaaaaaa cttcaagatt tgttttacaa
     4321 cttcagaaaa aaattatcta tatgtcaatt tggcaatgat ccatttgtat tcaagggttt
     4381 ttgggacaaa aaagaaggtg gatgcttttg gaaaatcagc agaagacaga gattttcagc
     4441 atatatttcc agtgatgtat ccaatcatgc aacagttttt tagtggggta gctcgcagaa
     4501 ttccagagct taatacttat tatgaaaaga acagacggtt gatttttcaa tactgtatgt
     4561 tagtcaggga tatttttata aaacacgaac aacctgtcat attctatatt caatcaaagt
     4621 atggtaaaac acaggaatta tgggcaaaga aaaggatatt atctatgact gaacgcccaa
     4681 taaaatatag tgcatccgct gatcagttac ctgatattgt cattagcgat tatcctatcg
     4741 acaaaaaggc atatgatcaa acgaatacac agatatttta ctggaatgga caacctacaa
     4801 aaaatgattg ggcgcgtctg aaagctgaaa tcataaaaat caggaatgag aaaaatagat
     4861 ccatattgga aaaattggat ttcgttaatt aagaaaagca gattgtttat gccaaaatta
     4921 agacattgca aaaagattat tttctttaaa atcgacaaga tgaccagaaa gcaagagttc
     4981 gttgagctct tgctttcttt tagttttaga aaggaacatc ctgattttat cttcgctaaa
     5041 tccagtcaaa agttttgcag attggtcgta gataatcggt tttttcaata aagaagggga
     5101 tgtacgaatc tcaatatata gttcactcaa actgagttca tctattctgt gaacaagatt
     5161 tttataacta atagaatgag tagagagcat ttcggtcacg ccgttgtttg tcaattttag
     5221 taagtggata aattcatttt tggttagtgg ttgacgaatt aagttctgtt cgataaacgg
     5281 tatatcatgg acgataagcc aacgttttgc gcgtcttgaa gacgagcagc cagaacctgt
     5341 atacaattta atcatcatac attcctccaa tagctaagta tttatgaaat aataacatga
     5401 agaaatagtc gaaaagtgcc atttagaggt gtaaaataag aaaatgttgt cacgtataat
     5461 ggaactagct agataagaaa tactattaaa tcaagaaaaa agaaagaagg cacaagtatg
     5521 tcaaacacag ccgacgcaat agtagtgatc gatttacaaa aaggggtatg ttttgattct
     5581 tcgccgatac acgaattaga aacagtgaca gcattagtga ataaaagaat caaaacatac
     5641 gaaacgattg ggaaaccagt tattttcgtt caacacgaag atgctcaatt agtgccacaa
     5701 acaaaaccat gggagctatt gtcggaatta tctattcctg agcacagtta ttttgtgaga
     5761 aaaacccatg cgaattcatt ttataagact aatttgagtg ttttattaac tgaattggac
     5821 tgtcattcga tcgaattatg tggggcacaa gcagagtatt gtatagatac gactgtgaag
     5881 tttgctcatg gattaggata ccagcttatg atgtgtagag gagcaacgac aacttatgat
     5941 aatcctttta tgaaagcaaa ggaaacaatc gctttttacg agaaaatttg ggataaacga
     6001 tttctgacat ttttggagca ataagttgtt tcttcgttta aaaaaagcag aatggatact
     6061 ggtttatcca ttctgcttta ttacgtaaaa tgagaaaata atcctgttct atttttatgg
     6121 gataaaccat ttattttttg tataaaaaat gtttgcgttt acatataatt ctcctagaaa
     6181 cataaaaagg agtgaatagc ttgagaacaa aacaagtatt aaagggagcg ttggtcatct
     6241 tatttgctgt aagtacaagc gtcccgcaat ggacagtata tggagagagc atttcttccg
     6301 aatcggaatc tcgggaggta gaaaccacag aaactactga tgctggaaat gaaacgaaat
     6361 cgagtacttc acaaacagaa acaaatactg aaacaacggt tgagacatct gttactagca
     6421 cagatcaaga aacgaccgct accacagata accaacaaac agaagaagca acagctacaa
     6481 aaatgtcaaa cgacgaatcc tcttctgcta aagtaagtga caaagagaat gggacagaaa
     6541 atgatgatat cggtaaaggt gaaaatttca atggtaaaga atatttgaat tacttcggta
     6601 aacagatgac agaaatactt aatggaccaa cagaagacat caagtcaaaa ttaatagagt
     6661 tattagtcac tgagcttgct gttaatgatt atggtgtaga taaagagatt atcaaacatc
     6721 tgattgctac ttatctattg tacgatatcg cttttgcaaa aaacagtgtg ttgatgtcga
     6781 tgcttggagg tacagatgat gggacaacgc ctacagaaaa cacaggcttc tatccaaata
     6841 caaaacaact tcatcaaact gtactaaaca aacaagatgg caaaatgatg gatgtatatg
     6901 catattatgt cgatcaaggt tcagataaaa cagtcatgat ccatggcgga tttcgaggga
     6961 attggaataa cgggatcgtt acagaagaat acaatgattt ttataaggcc gggtataatt
     7021 tattatttgt tgattccaga gcgactggta atagcggagg cgattatgtg acctatggac
     7081 aatatgagtc agatgacgtc ctttactgga taaatcaaga agttcgagag cggccatctc
     7141 aaaagatttt actttatggt ggttcgatgg gagcagctac gatgatgtct gtattagcga
     7201 aagatattcc tgtcaatgtc aaaggaatta ttgaaaattg cggatttgca tcaattgatg
     7261 aacaactacg ttttacgtac agccagacag tagcccccgc actaccggat gccataaaaa
     7321 atcagttaga catcattggg gatcaagagc atgaagattt atttatgggc cttttgaaac
     7381 aatactattt tgatcaggag atgcatttgg atacaaaagc tgctctacct acgattggaa
     7441 tgtcagattc attgcctaaa ttgattatcc atggaacagc agatgatgta gtacctgtgt
     7501 ctaacgcgca aaaattatat gagttatcag gagggtataa agatctttta ttagtagaag
     7561 gagcagggca tggaaaggca caggaagttg accatgctgc ctacacgaaa catgtaacgg
     7621 actttttgaa agtagtgttc catgatcaga taaacgtaaa gtatgtggat gaaaacaata
     7681 aaagtctact caaagaccag gacgaaatcc agctttacgg agcttatgga gaaaattata
     7741 tgacagagca aaagacattc gaaggatacg aattagcaaa tgttgaagga ccgactgaag
     7801 ggatttttaa tgagacgata ccaactatta ttttctatta caaaaaaatc ccagtagctc
     7861 caccaaaaaa gcaagatccg acagagaatg cagacaataa gggaaagaat gaacctcgag
     7921 aggatcaatc aagtgatgat acgcaaccga ttcagatgac agtgaaagta caaaaaaaga
     7981 cagatgcaaa aaaaagaatg ttgccgaaaa ctggagaaaa gaaacattcg cttctcattt
     8041 attcaggtgg atttttatct gttagcacga ttatcacatg gatttggcga agaaagagag
     8101 ggtaaatgaa tgaaatggtt caagcgaata gtgattgcag taccagttat catcgtagtt
     8161 attttagtca ctgtattttt gattaatcaa atcacaccaa agccgattag cttgcttgtt
     8221 agaaaacaat ttgacacaag tgaaaaagaa caagtatatg cccgaccaga tgatttccag
     8281 caaaaaatcg aacaaatcga tataaaaaaa gaaatatcct atccttcaaa gtacaaaaat
     8341 aataatatgg atatctacac accgaaagtg gaaaagaaaa aattaccgat tcttttctgg
     8401 atgcatggag gcgcatatgt aggaggggat aaaaatgatt gtcgtgatta tttggaatat
     8461 ctatgttcag acacgcagca aatcatcgta aatatagatt atgaacgatc accggaagca
     8521 aaacatccta caccagttat ccaattgaac gaagccattc aagcagctaa aaaagagata
     8581 aaagatcaag cagattggtc gaatatttcg attggtggaa attcggcagg tgcacaaatt
     8641 agtggagaat atttgttagc acttcaaaat gaagaaatta aaaaagctga tcatcttgat
     8701 ccgactttgg agaacaagca gattacgaag tttatttctt tgtctggtct gttggatcct
     8761 tctgcactta ctcaagtaag tgataaaatc tctagctttc tttatgaaaa atgtggatgg
     8821 gcttactttg ataataagga ctttgaacaa tcagagaagg tcaaaagttt agccttggtc
     8881 agacatgcgg atcgttggac gcaaaacaca ttcttaacgg atggaaatac aaatacgttt
     8941 acgaaacaaa tggaagaagt aacttctgta tttgagaaag cgggggtgaa agtcacaaaa
     9001 gtagattatg cggagaaaaa tgctgttttg aaccatgaat atcagtttga tttcagcaat
     9061 aaacaagcgc aagaaacata tcaaaagtta gtaatgtttt tcaaggagta aagtcttatg
     9121 atgcaaaaga ggttgtaatc tgtcacttag ctccaagtca caaggagcaa tttcaataaa
     9181 caagcttttc atggtttatg aatattgtaa cttgtgactg aggagttaga gatggcaagg
     9241 ggcacctttt attcgaaatt tgggaatatc acacgattat tgtcacgatc tcttttacgt
     9301 aaattttata agtcagattt gttgttttga tcgtttaaaa aaagcaacaa atccgaagcc
     9361 tgcggaggca agaagggaca aaaagccgag tggtaagcaa aagaatgctg gttccactaa
     9421 tattccgttg ttattgatat aaggctggga acaaagatga atagcaaata aaatcaatgc
     9481 actagcgaaa aaaagcaaag aaatatggat agctttcata agcagcacct ccttttttat
     9541 aattgtatga taaatagatt aagaatctat aaatgtgaca agtttgttag gatgtgaata
     9601 aaatgatcat ctagcgaact tgaaacaaag atggctaatg ataagcaatt aatgattcag
     9661 caatcagata ggtacaaagt ttaccatcat acgtctagac caataataga aaatatggga
     9721 atataataga aaagatttag gataccccca aaagaatttc cccaaaaatt cgatgtatcc
     9781 taaacttttt tattttacat aacaatcata tcaactgtga aataaaatta caaatgataa
     9841 cctaaaatga ggccaaaagc cagtgttatc aaggaaatac ttaattcacc aaaatttttt
     9901 ttaatttttt agaaaataag aaacaaaaat aataaaaaaa agaaaagata aattttttac
     9961 tactgtatac taactttgtt atcgcatcta acaagtaact cgaataatac caatacaacc
    10021 ttctccaaag atgcgatgac atacgttcta acccaccgac tattgatgtt ctgtaccacc
    10081 cttgcagaac caaaaaagcc actaccaaat atttggtagt ggcttttttt gttttatgga
    10141 tttttcgctt tttctgttat ggtgctattc tttgatttca gaaatagtat gtcgaacttt
    10201 agcgatagaa cgacgtgatt ctttttcgtt gtaaaacatc aaaatcacat atagagagtc
    10261 caaaatgctt agttgagaaa tacgggaagc taaagcttca gatcggaatt ctgtttcttc
    10321 agaaatagaa ataaaaacga catcgccaag tttagcaagg gaagaattag cttggctagt
    10381 aataacgatt gtttttgctc cagtttttct tacggtttca gcaatatgga ttgtttcttt
    10441 tgattgccct gtgtgagaaa tcaaaacagc acagtctttt tccgtcaata aagaagcttc
    10501 catcaattga atatggtaat ccgtgctgtg agaagttgcg ataggggaac gcaaaaattt
    10561 atgataagcg tcagttgcta caatctctga accgccaaca ccgaaaaaat agacacgttt
    10621 agattcattg ataatagtag ccgctttttt tagatccttt tgttgtaaca acttttttgt
    10681 gtcatttaaa gttgaaatac tagactcgaa caccttttgc gccatagtga gctcagtatc
    10741 tgttttctga atattttcgt ggatagaaat agcagataga tcattttctt ggatcaacat
    10801 ggccatttta aaatctttaa atccattata accaagtttt tttgtaaatt gaaaaaaagt
    10861 agagtcagca atgcccagtt cacttgctaa attgctaata ggactatgac ttactttgct
    10921 tggattttct aaaatataat cggcgatagc ctgttctttt gggctgaagt cgttgtacat
    10981 tgttttgatt tgtagtctta tggagacgct catgagaatt cctcattttc ataataagat
    11041 tatcattttt tccgttctca ttaatgttaa agatacaagg agagtaggaa aaaatcaaac
    11101 aaaaaatatt tttgtaaaat ctatttacaa aaaaattttt ttgtataatg agggtataag
    11161 aaatggaggc gctatcatgg aaattggtgt aattggtttg ggaaaaatgg gattgaattt
    11221 agctttaaac ttaaaagagc accatcacaa agtacaagga ctcgacattt caagtacagc
    11281 tgttagtcag gcaagagaaa atgacattcc tgcttatcaa gagatctctg aatttctagc
    11341 tacttttgag cagaaaaaaa tcatctggtt aatgcttcct gctggagaga taaccgaaaa
    11401 catgttaaga aaactttttc caaaactcgg accaggagac attattattg aaggcggaaa
    11461 ctcttattat aaagattcaa tccgtcgagc agaagagttt cagaaaaatg aaatcggtta
    11521 cttggattgt gggacttctg gaggaatcga aggagcacga catcaagcct gcttaatgat
    11581 tggaggagat aaagaaactt ttctatccgt ggaacaatta ttcaaagatg tagcaattga
    11641 aagaggatat ctgtatgctg gtgcatccgg aagcggacac ttcttgaaaa tgatccataa
    11701 tggaatcgaa tatggaatga tgcaagcaat cggtgaagga ttccaacttg tacaaaaaag
    11761 tgagtatgct tttgacttgg aacaagtcgc acgtgtttgg aatcatggtt cggtgattcg
    11821 cggctggctg atggaaatca tggaagatca gttaaacgaa catcccaatt taagtaatta
    11881 tcggggaatc gtttctgcat caggtgaagc aaaatggacg gttgaaacag cgctagaaat
    11941 ggctgttcca gttccagtaa ttgcaactag tttgttcatg aggaatcttt cacaggaaga
    12001 agatagtttt tcagcaaaag tagtttcagc actaagaaat gggtttggcg gacatgaaat
    12061 agttgaaaag ggtgagtaaa atgccagatt atcatatagc aatcgttaat tccagcagtt
    12121 tcggaagagt ctttccacac cacttggaaa gattaaagtc agttggagac gttcatttct
    12181 ttaatgttga tcaagagatt ccaggaaaag aactagcgga gttacttaaa ggattcaata
    12241 tcattattgc aagtgttacg ccattttttc gacgagattt ttttgaaaac aaagatgaac
    12301 tgctattgat ttcccgacat ggtattggtt acaacaatat tgatttaaaa gcagcaaaag
    12361 aacaccagac aatcgtatct atcatacctg ctttagttga acgaaatgct gtagcagaaa
    12421 ataatataac taatttgttg tccatgcttc gacgcacgac acaagcacaa gcaagagtca
    12481 aggacgatcg atgggaagat cgggcggagt tcgtcggtag gacgctgttt aacaaaacag
    12541 ttggtgtgat tggtgcagga aataccggaa gcggagtaat cgaaattttg cgtaacggtt
    12601 ttcgctgtga tgttctagct tatgatcctt acaaatcaga tttatacatc gaaagctttg
    12661 gtgcaaaaaa agtagaactt caagaattac tggaacaatc agatatcatt tgtttatgtg
    12721 cgaatttgac agaagagaac tatcacatga tttctgataa agaagtcgat cagatgaaag
    12781 aaaatgttta tctatctaat agtgcaagag gcgctctagt tgatgaagaa gcgatgatta
    12841 atggtctgaa aagcggaaag atcgcaggat atgccacaga tgttttagaa gaagaaccgg
    12901 gacgtcaaga tcatccgtat ttagcgtttg aacaagtagt gattacgcca catatatcgg
    12961 cctacacaag ggaatgttta gaagcaatgt gtgaaaaatg tgtcagagat gtcgaacaga
    13021 tcagtcagca ccttcttcct gaacgttctg tccagacaaa gagcaactat ataaaaggat
    13081 gatgaagaaa atggtgaacg gtttaaaagt gaaaacaggc cctcaatttt atttatatga
    13141 agaaggtggt atttcaaaag tttctgatct cttgaaatca tacggcgcga aaagagtact
    13201 ggtcacccac ggaacggtat catgggaaaa ggcgctacct aaattagtat ttttgaatga
    13261 cgaaacgatt caattttttt atcatcgtta ttctggagaa tgtagttatg ctgaagccag
    13321 acgaatagct acaatcatca aaaaaatgaa atcgatttcg tagttggcgt tggcggagga
    13381 aaactgaccg atctagtggg ttatagtgcc aatcttgcaa gaatcgattt cggagtcatc
    13441 cctactttag ctagtaattg tgctccatgg acacctcttg ctgtcatgta taaggagaat
    13501 ggacaagcag aagggaaaac agaacacttt tacagacaag ctgctttttt tataacagat
    13561 ccccaattag tgattgattc ccctgttcca tatttcattg ccgggttagc agatacacta
    13621 gcaaaatggt atgaatctga gctcatctta gaacaagatt ttttgcaaga tgaatcattt
    13681 ttgaagctag cacaagatac tgcgaagatc tgcaaagaag agattttgaa tcattctgct
    13741 aaagcaatcc aagatatggc acaaagaaaa ctgtcatcgg agttcaaaca gctttcagag
    13801 attatctttg ctgttgctgg tttagttgga ggattaggag ataaatacgc aagaaatgtg
    13861 gcagcacatg ctatgcatga cgctatcagt aaatatattc cggaaagcca tcgttttcta
    13921 catggagaaa aagtagcgta tgggattttc tatcaactgg cactagaagg tcgctgggaa
    13981 aaaatagacg agttatatcc tttgtatcgg gaattagaac tgccggtttc tctagaacaa
    14041 atgggattat ttccgaaaga taaacagata atagataaaa tggctgaatt tattgattcg
    14101 aaagagaaag tgcatttgat tcctatagag gtaacacaga aatcactgat acagtcaatg
    14161 atggaactgg aaggttatat cagaaagatc tgaaggagga ggacacatgg aaacattaag
    14221 tgtagtacaa agtttgttga ttgctttatg ggtagccgcg gttatgtccc gctggttagg
    14281 tggaggagcc acgttgacct tacgtttttc tcctttgatg actggattag tcgtcgggtt
    14341 gatcatgggg gaagttgcac aagcgatgat tgttacagcc gctttgcaaa tgatttatat
    14401 gggggttttt tctccaggag gatcaatgcc agctgaacca tctattgcag cagctatcgc
    14461 agtaccagtc gcattattag gggatctgtc gccagaagca gcgattgccg ttgcggtgcc
    14521 agtcggattg ctcggaagtt acttgtatca gtttcgtttt ttcatcaata cttttttagg
    14581 taaatataca gacaaagctg tagaagattt gaatacaaaa gctatggccc gatcaattat
    14641 tctttatcca acggttgcct catttttatt attcgttccg cttgttttca ttgctttata
    14701 tttaggggcg ccattgatcg ctgatgtaat caaagcttta gagggaacag tcgttatcca
    14761 cattcttgaa gtagtaggtg gagggttagc ggcaatcggt atcgcgacaa ctgtatacgt
    14821 tatcggtcga aaagattatc tggtcttttt cttccttgct tattttatga gtgttgtttt
    14881 gaaatcttta gatattacga tggttactta tgccattttt gggattatta tcgctttgat
    14941 ctttgtccag tcgcaaaaag ggaaacaagt acagcctgcc acaagcaata acaattcagc
    15001 atcttttgat gacgaagatg actatgatga cggattttaa tttaggagga atgggaaatg
    15061 gctgaaataa tggataatca aacaggtgct cctgaagaaa tcacgaaaaa agatgtaaca
    15121 aaagcctatc tgagatggca ttttgcaaac gaaattcctc actcttttga acgttacttg
    15181 gcgccatcac tactatacgg aatgatgcca atcttgaaaa aactttataa agatgaagac
    15241 tctttaaaag cagcatataa acggcagtta ctgttcttca acactcaatt aagctggggt
    15301 ggcggtgtga ttactggcct aatgtcttct atggaacagc aaagagcaga agaagagtat
    15361 gaagaaaaag agatcttgat gcaggatgac ttgatgtata acacgaaagc gggattgatg
    15421 ggggcactag caggaatcgg agatgcaatc gattcaggaa ccgtccaata tatttttatc
    15481 gcaatcgcgg taccttgggc acaacaggga agtcctttag gcgcactttt tccttttatc
    15541 tgctttgggt tgtatcaggt tttactaggg gtatttttcg caagacaagc tttcactatg
    15601 ggccgtaatg ctacaggcct aaagcaaaac actggtgttc agacagcgat tgaaatgcta
    15661 tcagttttag ggttgtttat gatgggggtg cttgcgggaa actatgtaaa agtagaatct
    15721 tcattgaaat ttgcgatatc cggacgtgag tttgttatcc aggaaattct tgatcaaatc
    15781 gtgccaggga tattaccttt agctgtcgta atgggtgtgt acttgttcta tacgaaaaaa
    15841 ggattgaaag tcacacaagc attattatgg ctgactggaa tattaattgt gctagcagga
    15901 atcggtattt tataaaaaga agggaacgtg aataagatgg gtgtagtcaa tttagcgcgt
    15961 gtggatgagc gtttgatcca tgggcaagta atggtaacgt tatctcaaaa aagtggagtg
    16021 aattccattt ttgttgtaga cgaagttgta gcaaaagata aatttatgag agatctttac
    16081 aaaaatgcag gaagccgtac aggacaaaaa acaattgtca ttacaccaga gaaagctaaa
    16141 ttctattggg atgaatatca atttaaagaa tacaattgta ttttgatcgc aaaaacagtc
    16201 caaacgattt acgacttagt aaaacacgga atccccatgg aagaattgaa tattggaggg
    16261 attgctcaaa aaaatcctga tacagatctt ttagtgacga aatctgttta tttaaataaa
    16321 tcagatgctg aaaaattgaa agaaatgaat gaacaatatg gcgtaaagaa tatttatttt
    16381 caagccactc catctgcacc taaaacagga ttaaaagatg tattgaacaa gtttgatttg
    16441 tcataaacaa atgcttagaa aaaagataaa gagggcgaaa aaatgaagaa gaacgagctg
    16501 tttcgtgatt gggaatttcg ttatcgctat atttaccgta agcgccgtac aaaaaagagt
    16561 aaacagcgtt ttctttcagc attagtatca gatatttaca gtatgagaac agatgtgaca
    16621 gtcattgctt acgatacgct agcctaccga tcaaaaaata tttatgttgg agatatcgaa
    16681 aaagcagaga aagttatctg tacttattac gatacacccg ttcatgcatt aggaagttat
    16741 ttcatgtttg attggaaaga ccaaaggaaa aaaacaatct actctatatt attgtccttc
    16801 attttgttgt tcagtttagg ttggtgggga atgatgatat ataacaaaaa tcctcatcac
    16861 gtattcgatc tgctatccgt ataaacaggt atcactgtac tagcatttgg gagctacttt
    16921 ttcttattgg gaaaagcagc tcgcggatgg cctagcagac aaacttttat tcgaaatact
    16981 tcatccatat taacgatgct agaaatgatc cgtacgattg atgatccgag tgtagcgtat
    17041 gcgtttgttg atgaaggctg ttatggagaa aagggcttag acagcgtgag atcgggtatg
    17101 aaaaaagagg ctattctttt ttatctagac tctgttgggg cagatacacc gctacagttt
    17161 tcgggaaatt atttttcaaa taaagaacag tggctgaaac aagttgataa gttgaaagaa
    17221 aaaaacgtca attatatttt tagtgcaaga aaaaagcaag cgcagttctt ctatttgacc
    17281 aaaacagatt tacgagggaa aacatttaat tggcaaaatg ccaatcagat tattgctctt
    17341 tttcgataag taaaaagtgc aaaacaagga gtgagaagac taatgttgga aatcatgatc
    17401 gttacacatg gtaagttgag cgaaggactg aaagatgcgg cggaagtaat tgtcgggaca
    17461 tcgagccata ttgaaacaat gtgcttagag tctggggagg acatccaaac attcggagag
    17521 aaggtcaaag aaaaattaac tatgatcaac acagcagaac ccatgctagt atttgttgat
    17581 ctaattagcg caagtccgta caatcaagcg ctcttggcag tcaatgaact gactgaagaa
    17641 aagaaagcag ccacttatgt catagggggc gtgaatctgc caatgttgtt agaagcaatc
    17701 aatcatcaga tgcttcacac accgatagaa gaagcggtgg aacaaattgt caaacaagga
    17761 aaagataatt taactgtttg gcatgcttca gcggaagaaa aaaaattaga tgaggacgat
    17821 ttttagaagg aaggctgaaa agatgaagtg gggatttaga tggtatggtg aaaaagggga
    17881 ttcgatccca ttaaaatatg ttcgccaaat accgggaata aatgggataa ttggaacttt
    17941 gctgactaaa atgcctggag atgtttggga gatttcagaa attgaggagt tgaaagcatc
    18001 tgttgagagc caaggactgg aactgcttgg aattgaaagt gtagcgatcc acgatgcaat
    18061 caaggcaggg acagatgaac gagattacta tattgataac tatatacgaa caatcaaaaa
    18121 cttgtctcga tgtggggttc gattgatttg ttatagcttc aaaccaattt ttggatgggc
    18181 gaaaactgat ttgtttaatg aaaacaacga tggaagctat tctcttttat acgatcagaa
    18241 aatcgtcgat cagatggagc caggagaaat gtatcaattg atacacagcc agtcaaaggg
    18301 attcaaattg ccgggttggg aagaagaacg gttgaaaaaa ttcgctcagc tggaaaaact
    18361 gtatgaagga atgaccgaag aagaattatt tgaaaatctg acttattttt tagaaaaagt
    18421 gattccagtg tgcgaaaaag cagatgtaaa aatggctatc catccagatg atccgccatg
    18481 ggaacttttt ggttttccta gagtgacgaa aaatcttggc gacttgaaga aaattctaaa
    18541 tgcagtagat tctccatata atggactaac gctgtgcaca ggatctttag gggcaaatcc
    18601 ggaaaacgat ttagttgaaa tcatccatga ggtaggttcg cgcatccatt ttgtccactt
    18661 cagaaacgtg gagtttttag gagaaagaaa gttcaaagaa acggcacatc cctctacaga
    18721 aggctccctt gatatgtatc atattatgaa agcgttggtg caagaagggt tcgacggtcc
    18781 tatacgccct gatcatggta gaaccgtgtg gggtgaagtg gctatgcctg gatatggcct
    18841 gtatgatcgt gcaatgggac tcacttacat gcaaggtcta tatgaggcga taagtaagga
    18901 aaaaccactg ggagagcatt atgaataaaa tcaagctctt catctttgat atggatggcc
    18961 ttttattaga aactggacgt ttagcttatc gtgcttatgt aaaatcagca caaaagtatg
    19021 attatgaaat gcgtaaggaa gtctattatt tactaactgg ccaaacagaa atggcgataa
    19081 gagaacaaat gggcattttg tacggagaag atgttccata tatcaaatgg cgagaagcaa
    19141 tcaatcaata taaagaaaaa atcgtgaaag aagataagag agtatatacg aaaaaaggag
    19201 cagaagaaat tttatctttt gcaaaagaaa gaggaatcca tacaatcgtg gcttcgtcta
    19261 atacacgggt aaaagtagaa atgtatttaa aaatggaaaa tctatatgcc ttgtttgatc
    19321 aaatcatttc aggggatgat gtaaaaaaag gaaagccgga accagagatc tttttgaaag
    19381 cttgttcaaa aatgaatatt cctccaagtg aagcacttgt atttgaagac tcgattgcag
    19441 ggatcgaagc agcaaggcga gcgggcattt tgtcttttct catcaaagat catttgatag
    19501 ggttgcccga tcataatggg aagcataagt tgaaagtaga tgtaagttta tatgaatcca
    19561 ataaagcaga ctatgaattt tatgacctgc atcaagcaaa acgattttta gaaagtaaag
    19621 ggttgtatct ctaataaaga ggactatttt ctagtcctct ttatttattg tcctgcacga
    19681 ctgagggaac agatgaaaaa gaagtaattt tttattttga agtatctacc ttatttgtag
    19741 tgaacaattt agaacaaaca caaaacgcat ctactgtt
//
LOCUS       DAZULG010000051        19575 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000051 DAZULG010000000
VERSION     DAZULG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.052"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(540..1565)
                     /locus_tag="ACG9CY_001581"
     CDS             complement(540..1565)
                     /locus_tag="ACG9CY_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4938093.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1840..3525
                     /locus_tag="ACG9CY_001582"
     CDS             1840..3525
                     /locus_tag="ACG9CY_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS4938094.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3539..4822
                     /locus_tag="ACG9CY_001583"
     CDS             3539..4822
                     /locus_tag="ACG9CY_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4938095.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4892..5761
                     /locus_tag="ACG9CY_001584"
     CDS             4892..5761
                     /locus_tag="ACG9CY_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4938096.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5758..6594
                     /locus_tag="ACG9CY_001585"
     CDS             5758..6594
                     /locus_tag="ACG9CY_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4938097.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6620..7687
                     /locus_tag="ACG9CY_001586"
     CDS             6620..7687
                     /locus_tag="ACG9CY_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS4938098.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7684..8511
                     /locus_tag="ACG9CY_001587"
     CDS             7684..8511
                     /locus_tag="ACG9CY_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4938099.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8556..9581
                     /locus_tag="ACG9CY_001588"
     CDS             8556..9581
                     /locus_tag="ACG9CY_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4938100.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9578..11761
                     /locus_tag="ACG9CY_001589"
     CDS             9578..11761
                     /locus_tag="ACG9CY_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS4938101.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11761..12891
                     /locus_tag="ACG9CY_001590"
     CDS             11761..12891
                     /locus_tag="ACG9CY_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4938102.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12888..13565
                     /gene="pgmB"
                     /locus_tag="ACG9CY_001591"
     CDS             12888..13565
                     /gene="pgmB"
                     /locus_tag="ACG9CY_001591"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4938103.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13649..14176
                     /gene="msrA"
                     /locus_tag="ACG9CY_001592"
     CDS             13649..14176
                     /gene="msrA"
                     /locus_tag="ACG9CY_001592"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4938104.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14377..15063)
                     /locus_tag="ACG9CY_001593"
     CDS             complement(14377..15063)
                     /locus_tag="ACG9CY_001593"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS4938105.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15143..16681
                     /locus_tag="ACG9CY_001594"
     CDS             15143..16681
                     /locus_tag="ACG9CY_001594"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS4938106.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16801..17268
                     /locus_tag="ACG9CY_001595"
     CDS             16801..17268
                     /locus_tag="ACG9CY_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS4938107.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17802..18230
                     /gene="rplM"
                     /locus_tag="ACG9CY_001596"
     CDS             17802..18230
                     /gene="rplM"
                     /locus_tag="ACG9CY_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS4938108.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18246..18638
                     /gene="rpsI"
                     /locus_tag="ACG9CY_001597"
     CDS             18246..18638
                     /gene="rpsI"
                     /locus_tag="ACG9CY_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4938109.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18726..>19575)
                     /locus_tag="ACG9CY_001598"
     CDS             complement(18726..>19575)
                     /locus_tag="ACG9CY_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4938110.1"
                     /translation="NTEIISDELTKKSKEYANIKIIKGYVRSMFDIAEILNYIEFNRT
                     TKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMI
                     TLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTL
                     LSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRHPDL
                     VHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIVDKAVFEAFQR
                     GLNKCD"
BASE COUNT         6338 a         3533 c         4152 g         5552 t
ORIGIN      
        1 tctaaaataa tttgtctaac tagaagaaaa gtgggaaagg atcttaacaa catcggttcg
       61 tatccaatta aggtatcgga acgatcatct tccaagagaa gtcaatcaac aaaagtaaag
      121 atagatcatt ttttgaatgc aaaataaatt gatgggaaga tagagcggtt atttttaata
      181 ttttgctagc tattattgaa taattcctcg aataaaacaa aaccagtaga catgtctgtt
      241 attctattta tgttttagag ataagtatcc ccaaataaca tggattgaaa taagggtaaa
      301 tgtgtttaaa aaaacgaact ataaaaactt atacacaacg tggttaaaaa tagttttttc
      361 ttccctgtgg aaaaaatagt ttaacacaaa cattatagtt atccacagct atctaaaaca
      421 gatggaaaat aagaaaaatt cgctaaaata ccgggagggg gtaacgaggg atgagtgatt
      481 gaaaaaagaa cagagtaact gacttatcct cagtgtttct gttcttttat aattctgttc
      541 tacatttctt gttgttggat cgaatctgtg agagtaatct cggttgaaag aatgattctt
      601 ttttcttcag ggtattggtg gtggattagt tggaataaaa gttctgtggc tttgattcct
      661 aactgatttg tcggaatgtc tatccgagtc aactgtggcg aaaataggga ggacaataag
      721 ggatcactga atcctgcgat cgaaagatca tcaggtatct gctttccaga ttgttctgcc
      781 actttgtaaa tactcaatga ttttaatgca tcggtagaaa aaatcgcagt tggtctttct
      841 cgtagcttca ttaaatcatt cattgctgcc atgctatctt ctaagcgaaa gccgccgtct
      901 ataatccatt cattacggat atctaggtgg tgatcaaata agcattgacg gtaaccattg
      961 agacgatcat tggatacata aacttctgtc ggagcatgga gacagccgat atgacggtgt
     1021 ccattcttga tcagtgtttc caccaaatcg taactatcct ggaaattatc cgtgtccact
     1081 gagtagatgt tcgtgtagtc gtttccgact tttccgatta ccacaatagg aatattgtat
     1141 tgatccaatt caaaaagaag tgtttcatcc atcgtagagc ttaaaataat aattcctcgg
     1201 atcatttttt cttggatctt tttcgcgcat ttttctagct cttcttctgg tgtttttgcc
     1261 gtttgtagaa tcaagtcgaa gttattttgt tcagaaacgg cagaaatgga ttgaataaca
     1321 tctgaaaaaa aaggattgcc ataggtcgta tgagaagtac gatgagataa tgctagaatc
     1381 gcgtcaaaac catttcctga cagtcctctt gcaattttgc tcggttcata attcaattct
     1441 ttgattgctt gaagtacact tgcttttgct ttatcagaga tatttggctg gttatttaat
     1501 actctggaga cagttgattt tgagacatta gcttttttcg caatgtcata aatagtaggt
     1561 ttcaatttta tatgcacctc ctttttggga gcgttttaga ataattccat agtacaatag
     1621 acattctttt ttgtctatga ttttgaaaat ttaggaatag cttgcatgat catgagagac
     1681 catatttttt taagaagcta ttaattattg atacataagc tttcttattt gtatttttgt
     1741 gtatatcaaa attatttaaa aaaatgctat tgacagtaag cgttttccat aatatgatca
     1801 aatcgttggg aacgctccca aaaaagaaag gggaatccga tgtatacaaa acttttacaa
     1861 gcgatttatc gcgactcaga tttgtctgag aaaaccgcct ctatgattga acaaaaagtc
     1921 agtgtttatc agcagaagaa aggccaggca ggcaaactgc attgggacga agaagatatt
     1981 ttcttgatta cttatgggga tcaatttttt gaagagcagc aaccaacttt gcgtacattt
     2041 aagaaatttt atcaaaagta tttggcagat acatttagag ttgtacattt cttgccattt
     2101 ttcccatata catctgatga tggtttctcc gttgtaaatt atcaggcaat caacgaccgt
     2161 ttgggcacat gggaagatat agaagaaatg agaagcgaaa cacggttaat gttcgacttt
     2221 gtctgcaatc atatgtctgc cgaaagtact tggtttcagg aatatttaaa cttgaatgat
     2281 acgtacaaag atttttttat tgatttgcca ctggatacag atacaagcag cgtgactaga
     2341 ccaaggacca gcccgttact tacaacgttc attgacaaaa acgggaaaga aaaacacgta
     2401 tggacgactt ttagcgaaga tcaagtagac ttgaatttta aaaatccaga agttttattg
     2461 aaaatgatcg atattctttt gttttactta gaaaaaggcg cttcatttat tcgcttggac
     2521 gctgtgggat ttttatggaa agaactagga acttcttgta tacacctgcc agaaacgcat
     2581 aaaatcatcc aattattccg tgcaatcgct gatgaagcag ctccaggaac ggtattgatc
     2641 actgagacaa atgtgccaca caaagataat atcagttatt ttgggaatgg cgagaatgaa
     2701 gcacagatgg tctatcagtt ttcgctacca cctttgaccc ttcaagcgat tcgttcgggt
     2761 aatacaagta agttgaaaga atggttaaaa accatcgatt tcgcatcgaa aaaaacaact
     2821 ttttttaatt ttttagcctc ccatgacggt atcggattga atccggtacg aggtattctg
     2881 ccggaaaaaa cgatccttga attagtggaa cagctgaaga aagagggtgc ccgagtaaac
     2941 tacaagaaaa acccagatgg aacgaccagt ccatatgaga taaacgctac gtatctagat
     3001 gcgttaagtt ctccaacaga tacgacggaa tggagaagca aacgcttttt agctgctcat
     3061 tcatttttgg taacgttacc aggcgtgccg gcaatttata ttcaaagtgt gctgggaagt
     3121 cgaaatgatt acaaaggcgt agaaaaaaca ggagagaatc gaaaaatcaa tcggcaaaaa
     3181 tttttgttag cagatattga gaaagagttg cagacagatc cattaagaac agcagtatat
     3241 ggtgaattga cccagatgct atctattcga aaaaaagaaa aagccttcca tccgaatagt
     3301 ttgatttact tactggactc agcagattct tttatcagtt ttattcgcga agcgaaggaa
     3361 gagaaagtct tagttatcca taatttttcc aatcaatcag caggttatct tttaccaaaa
     3421 ggagattacc tagacctgtt ttctggacaa attatccatt gcagtcaaga acgaaagttg
     3481 atattccagc cgtatgaata tatgtggctg aaaataataa aataagaggt ggaaaaaaat
     3541 gaaaaaaatt attgttacta cctgtctgct agctactgga tttcttctta ctgcttgtgg
     3601 tgaaagtcaa agctctaacg aatcacaaca gacaacgatt caattcatgc actcttctgt
     3661 cgaacaggaa cgattaagtg tgatcaatga actggttgct gattttgaaa aagaaaatcc
     3721 agacatcaaa atcaagcaag tgccggtaga agaatcagcg ttcaatacaa aaatcgttac
     3781 gttagctcgt tccggtaaat taccagaagt aatggaagtc agtcaggatt ttgctaaagt
     3841 gatggataaa gatgaactga ttgatcagac ggcagtccaa aacgttatcg aggaagctgg
     3901 agaagataat tattatgacg gagccaaaaa ccttgttcgt tcagaagatg gaaaaagcta
     3961 tattgctgca ccaattagcg gctgggttca aggtatttgg tatgacaaag aaaaattatc
     4021 agaagctggt ttttcagaac cagaaaattg ggatgatatt ttaaaaattg ctcaacattt
     4081 cacagataaa gagaataaac aatatggaat tgctatgcct actgctgaag gaacaatgag
     4141 tgagcaagcg ttctctcaat ttgctttgtc aaatggagca aatgttttgg atgatgaagg
     4201 cgaggtaacg atcgatacgg aaaagatgag agaagcattg tcgttttatc aaaacttatc
     4261 ccaatacacg atgccaggct ctaatgatgt gacagaaatc aaagatgctt tcatgaatgg
     4321 aacagcacca atggcaatct attcaactta cattttgcct tctgtttatg aagcggggaa
     4381 ctcagaaaat atcggttttg ctatcccaac acaaaaagat caagctgtct atggcacagt
     4441 atctggattg acgatttctt ctggtctaga cgagaaacaa aaagaggctg ctgaagcatt
     4501 cacagctttc ttatctcaac cgaaaaacat ggaaaagtgg gtcttgatgt ctcctggtgg
     4561 tgctcaacca gtgaataaac aagtagtcga actggatggt tacaaagaaa atgaagtcat
     4621 caagtctttt ggcgagctgc catctgagat tgctagtgct tttgatgaag tacaagtctt
     4681 tggactagtc ggggaaaaga atttcacgaa aatgggagat atcacaagct caggtgtgat
     4741 cggaaaagca gtgaaccaag taactgtcgg aaacgaagat gtcgatcaag ctttagctga
     4801 cgcacaaaaa aatacgaaat aacaagaagt tctgccaatg gtgttcgaaa aaagtagata
     4861 gctattggca gattatcagg gggaggtttc aatgaatcgt ccaaggaaac attctgactt
     4921 aaaattagcc atgggacttt tagcaccaag catgctttta ttagctgttt tagtgctgat
     4981 tccaatgctt tccaatattc agatcagttt tttcgatatg ccgctgaatc ctaactcaga
     5041 cgccgttttt atcggattaa gcaattatct tgaaatttta agtgatagtg aattttatcg
     5101 ttctttaggt ttaacagttg cttacacatt cttagttgta gcaggcagta cagctttagg
     5161 attagccgta gctattttgc ttaaccgcaa gttcagattt aggaaaacgg tgcgttcact
     5221 gattttatta agttatgtta cgccatctat ttctttgatt tttgcttgga aatatatgtt
     5281 caataatagt tatggaatcg tcaatgatct gttagtagat aggttgcagc ttttcaacga
     5341 agcaccgctg tggttcgacc agccagttgc tagcttcttt atggtcgttt tgtttgctat
     5401 ctggagatac tatccgtatg cgtttatttc atttttggct attttgcaaa cagtcgacaa
     5461 aacgctttat gaagcagctg aaatggacgg tgcaaatgct tggcagaagt tcaagatcgt
     5521 gacactgccg gcaatcaaac cagtactagc aactgtgatt gttttacgat ctatctggat
     5581 gttctatatg tttacagatg tctatctact gacgaataaa gtcaatatct taggcgttta
     5641 tttgtatcag acagcctttg cgttcaatga tcttggaaaa gcagcagcta tatcgattct
     5701 attgttcatc attattgcag cggtcgtctt gctctcacga aagaaggtgg acttgaaatg
     5761 aaaaataaaa agagcaggaa aatcttattt tacgcgacga ttttgttgct attagctgtc
     5821 aatttgtttc catttttgat catgctgatg acatcattca aggattcaca agaagcaatt
     5881 gcaacgacac aaactatttt tccgaaagaa tggacttttg aacattatca ggatattttc
     5941 aatgctgaga tttttccatt catcatgtat ttcaaaaata gtttagtgat ctccttgata
     6001 gcctccgtaa ctgcagtagt gattgggatc agcggggcat acgctttgtc aaaactacgc
     6061 ttcaaagctc gctcgaaaat caatgctagc ttttacaccg tttatatgtt ttccggtatt
     6121 ttgatcgttg ttccattatt taaaatcatt tccggtctag gtttgtatga taccaataca
     6181 gcactgatta tcacgatggt cgttcaaaca cttcctactg cgatattcat gctaaaaagt
     6241 tattttgaca cgattccgac tgatattgaa gaagcagcaa tgatcgatgg tcttagccgg
     6301 atccagatca ttattcggat cattatacca ttggctattt ctggaatcat ttctgtcttc
     6361 gtttattgct ttatggtttc gtggaatgat tatttatttg cttcgatttt cctgtcgacc
     6421 tccgaaaaat ttactttacc aatcggtttg aacacattat tcagtacacc agactacatt
     6481 tggggacgaa tgatggccgc ttcattggtg accgctttac cagtcatcat tatgtatgcg
     6541 ttatctgaac gatttattaa aggtgattta gtcgaaggcg gcgtaaaagg ataaaaagaa
     6601 aagaaagagg aaagaaaaga tgaaaaaatt agttgcatta gaaccaagaa tagccggatt
     6661 caaagattac gaagaccgtg agatcgaaaa gaacgaagta cgtatccgag taattttcgc
     6721 tactccaaaa ccaggaacag aaattgtcga ctttagagga ttaagtccat ttatcgatga
     6781 agaattttca gaagaatggc gcctattctt gccaagagaa gaaggaagcc aacgcggtgt
     6841 ggtatttggc gaatttcaat taggaaatat gattgtcggt acgatcgaag aaacgggcag
     6901 tttggtgaca gagtatcaaa aaggagagtt agtctgcact tatggaccca ttcgtgaaac
     6961 gctgattaca aacggtgttg ataattataa actacgtaaa cttgatcggt tggatcaatg
     7021 gaaaaatgca gtatgttacg atcctgctca attcgcttta ggtggtatcc gggaagcaca
     7081 tgttcgagca ggtgatgctg tagcaatctt cggtttaggc gctatcgggc aaattgcgat
     7141 tcaattagct aaaaaagcag gtgctactac catcgttggg atcgatccca tagcgcagcg
     7201 tagagaatta gcagaaaaat acggtgccga ttacacgcta gatcctatga aagcagatgt
     7261 cggttttcgt ctaaaggaat tgacagataa acgtggagta gattcagtta ttgaaacgag
     7321 tggcagtgct gccgcattac aagcttcttt aagagggatc ggctatggcg ggatcatttc
     7381 ttatgttgcc tttgctaaac cttttccagc tgggtttaat cttggtcgcg aagcacattt
     7441 caataatgca aaaatcgtct ttgctcgagc atctagtgaa ccaaacccag attacccaag
     7501 atggaaccgt aaacgaatcg aggacacatg ttgggaatta ttgatgaacg gttacttgga
     7561 ttgtgaagaa atcattgatc cagttgtgcc atttgcagaa agcgcagaag cttacatgcg
     7621 ttatgtagac cagcacccag aagaaagtat caaaatgggc gttcaagtcc gaggaggaga
     7681 acaatgaaat tcggtacgca agatcaagca tttttcccaa gagacatttt agaaaaattt
     7741 agatttatca aagactgtgg atttgaagaa tacgaaatcg acggtaagtt gttaatagaa
     7801 aatatcgaca cagtcaaaca ggcaatcaaa gaaacaggga tcccagtagt gacagcttgt
     7861 aacggctacg atgggtggat cggtgacttt atcgaagaac ggcgtcttaa tggtgtacgt
     7921 caaattgctg agattttgcg tgcactaagc gaggttggag gcaaaggaat cgttatccca
     7981 gcagcttggg gaatgttcac ttatcgatta ccgccgatgg tttcaccccg aagcaaagaa
     8041 ggagactata aagcagtcag cgaatcactt cgttatttag ataaagtagc cgaagaaaca
     8101 ggtacggtta tttatttgga gcctttgaac cgataccaag accatatgat caatttgtta
     8161 gcggatgcgc gtcattacat tgactctaat caattgaaac atgtgcagat catcgctgat
     8221 ttttatcata tgaatattga agaagatgat atttcaaaag cattgactac gcaaaaagag
     8281 tatgtgggtc atatccattt agcagataat catcgctacc aaccaggatc tggcgcaatt
     8341 gattttactc gccattttca aacgctactt caaaatgagt atgaaggtta tatggtatat
     8401 gaatgtcgtt tgcgaggaga aaatgaagtg aaaatttatc aggaatcatt gcaatacttg
     8461 aaaaactgtc tggaaacagc gcgtcattta aatgatttag agaaagaata gagaaagaat
     8521 aacaataagt aaataagcaa aggagagaga aataaatgaa gaaagtagca atcataggcg
     8581 ctggacaagt agcagaaaaa gtacatgctg cttattatca aaaaagatca gatgtcttag
     8641 aattggccgc tgtggttgat ccagtcagcc agcgagcaga agacttttgc cgacgtacag
     8701 gattttctca tgcgtataca tcagttacac aaatgctgaa tgagatttct cccgatatcg
     8761 tgagtgtctg tacagccaat cgctttcact tcgaacatgt attggctgcc ctcgaagcta
     8821 atgcttcagt gatgtgcgag aaaccaccag ctatgacagc tgatcaagca agaaagatgg
     8881 cagagacagc cgataaaaac gggcagattt tggcttatgg attccagcat cgtttttctg
     8941 acgaagcaac aatgatcaag aatcatcttg atgttctcgg ggatatttat tacgtagaag
     9001 cgaatgcgct tcgtcggtct ggtgttccag gctggggaac gtttatcgac aaagaacttc
     9061 aaggaggcgg gcctttgatc gattatggca ttcacatgct tgatacagcg atgtatatta
     9121 ctggatttcc taaagtaaaa agcgttcggg cgtacagctt ccaaaaaatt ggtacgaaaa
     9181 aaaattcagg gaaattcggg aaatgggatc caagcaaatt cacagtagaa gatgcaattt
     9241 ttggcacact ggaattagaa aacggcaaga tcatccaact gaatacttcg tttgctttga
     9301 atatcagaga agacaaactg aatgtggctt tttgtggcga tcaggcaggt gctaatctgt
     9361 atccaggcga actattcgaa gatgtagatg gaaagctggt ttccttacaa aagaccacgc
     9421 cagttaatga cccacatgaa ctaagtatgg ctcattttgt gaatcgggcg ctcggtgacc
     9481 agactcagat gattgcagat ggctggcaag gatatgtcat ccagaatctg gtggaaaatt
     9541 tatatcgttc ggcagaaaca ggagaagtta ttaccttatg aaagtagaag aaaaacagtt
     9601 tgatttagag tatatcaata agtatgcgag tcagatgacc attggaaatg gattttttgg
     9661 tatcagagga agccaggagg aagactatcg gaatcaagta aggggaatgt ttgcagctgg
     9721 tgtatataac cgacctttag gagcagaatc agccgagtta gtgaatctcc cagatgtcat
     9781 cagatgggaa ataaaaatcg acggtgaaca tttttctctt gagacgatga aagttcttgc
     9841 atacgagcgg gcactagatt tagaaatggg agagcttaaa cgctatgcgc tcattgaaac
     9901 tgctcaagga aaaaaagtgg aagtcattac ttgtcgtgtt gctgatcaaa ataatctcca
     9961 tcaggcagcc ctgaaagtgg tcatcaagcc gttgaccgaa gactgccaag tagatgtgaa
    10021 aacgggtatc gatggacagc aaaccaattt tggtacacag cagttggttg aaaaagaact
    10081 gcgagtattt ggtgacgatt tgatcagtgc atcctaccag acaacagaaa gtaaaattga
    10141 tattgggatt gcagctaaat tcaataaagc aggtgtattc caagcaaaaa atcgccggat
    10201 caccgcttcc ttttcagaaa agatcagaaa aaatacgtcc tttacgctgg agaagatcat
    10261 cgtgctctct acctctcttc agcaaacaga cccgtttaag aaaagtattg cccaagccca
    10321 gaaaagcgtt agttatacag aaattttaga gcagtcgatg tcttattgga cagatttttg
    10381 gcatcagcat cggataacga tccaaggcga ttcttctttc gatcagttag cgatggactt
    10441 cgctgcttac catttacaga ttatgactcc ccgcaatgat ccaagtgtta gtgtaggggc
    10501 gaagggacta actggcgaag gttataaagg gcatgttttc tgggatacgg aaatttttat
    10561 cctgcctttc tttttatatc agtttcctga agtagcaaag aatttgctgc tgtaccgtta
    10621 tgatcgatta gaaggcgcac ggcaaaaagc aagagattat cactatcaag gtgcactttt
    10681 cccatgggaa agcgcacaca ctggtcagga agagacaccc aaatttgctg cattgaatat
    10741 caaaaccgga acaagacaag aagttgcttc agctgccgca gaacatcata tcagtgcaga
    10801 ccttgcttat gccgttcaag agtatgtcca agcaacagag gatcaatcgt ttttagaaac
    10861 aaagggaaaa acgctcattc atgaaacagc agctttttgg ctgagcagag ctgtaaaagt
    10921 cgatgatcat ttagagatac acagagtcat tggtccagat gaatacacag aatatatcga
    10981 caataatgca tatacgaact atctggcgca ttataatgtg gccttagctg ttaaatatca
    11041 agctggcgat ccgctttttt tgaaagaatg tcaaaatttt ttagataagt tatatttgcc
    11101 agtgcctaac aaagaacagc tgattccgca agatgacacg tatctcagta agccagttat
    11161 tgatttgacc acttacaaag aacgtcctgg cagccagtct attttacttg attatgcccg
    11221 tagcgaagta aatgaactcc aagtcagcaa gcaagcagat cttgtcatgc tgttatactt
    11281 attacctgag tatttttctc aaccaatcgt ttctaagaat ttgcattact atgaaaacaa
    11341 aacaatccac gactcatcgt tgagtaaagc gattcacgcc atagaggctt gccgtatcca
    11401 agaaatggaa tgggcgtatc atttattcca agaagcgtgc ctgattgatt taggaccaga
    11461 tccgcattca tcagatgacg gaattcatgc tgcttcatta ggagcactat ggctagcagt
    11521 tatatttggt ttttcaggta ttacagtaac gaatggaact ctttctattt ctccaagact
    11581 tccttcagca tgggaaaaaa tttcttttcc ttttatttgg aaaaatcaca ggttgctatt
    11641 tgttttgaca aaaaaacaaa tagtaataaa aaaagagacg aaagaaacat tacctatttt
    11701 tgtcaatgga gaccaatacc agctggaaaa agaactgatc ttggaacgga ggggaaaata
    11761 atgcgtattc tgattgcgcc agattctttc aaagaaagtc tgacagcctt agaggcagca
    11821 caagccattc aatcaggttt tcaagaagtt tttgttcagg ctgaatatga tctgctacct
    11881 ataggcgatg gaggagaagg aacggtttca tcattagcag acagtttaag gttgaagcgg
    11941 caaacagcag tcgttactaa tgcatttggc aaaaacaaag aaatccagta cgcttcagac
    12001 ggtcaaactg cgatttttga aatggcagaa atcgtcggat tgaaagatgt cccactaaac
    12061 caacaacagc cacttaggtt atccacgcga ggagtaggag aactgatacg acaattgttg
    12121 caacaaggtg ttcgacacat catcatcggc gttggcggaa gtagtaccaa cgatggcggc
    12181 atcggcatgg cagaaggtct aggttatgct ttttacgatc aagataatca aaaagtagaa
    12241 gcactgggag aaaatctagg taaaattgct acagttgata aaacgaacgt gcctattgag
    12301 ctgattaaag ccaaaatcac tgtcattact gatgttacta atcttttatg cggaaaagaa
    12361 ggcgcttctt ttgttttcgg accacaaaaa ggcttgaaag aaaatgaatt agcagtggct
    12421 gaccaagcca tgcatgattt ttatatgaga tttgcccctc aagtaatcga ggcatcgggc
    12481 agtagtgctg gtggtggaat gggtgcagga ctgttagcat ttgcttcagg gaaaatagtc
    12541 tctggcatcg actacgtatt ggatttactg gagttcgata aacgggtaca gcaagcagac
    12601 ctcgtaatcg taggcgaagg acgtctagat ggacaaagtc taaaaggaaa agcgccagtt
    12661 gggattgcta gaagagtacc agagggcgtt cctgtgatag ctatttgcgg aagtgttggt
    12721 aaaggaagcg aaaaagcagc agatgttggc atttcggccg tttttccgat ccttcctgca
    12781 gctgtgcctt tacaagaagc tttacagcaa gccactgata atcttcatcg cacagctaaa
    12841 aacatcgcat ctttacttca agtcgttcat tttcaaggag gtaatcgatg aaaatcaaag
    12901 cagcactatt cgatttagat ggcgttcttg tagacacagc tcgttatcat tacgaagcgt
    12961 ggctggtttt agctaaccag ctgtccattc cattcactga gaaagaaaac gagcaactga
    13021 aaggaatatc cagaaccgag tcactagagc gattgctgtc atttggaaaa atggaacaga
    13081 aattctcaga aaaagagaaa tcagcctttg ctgagcaaaa aaacaatcta tatcttcaag
    13141 cgattcaaaa aatggatgaa acatccgttt tgcctggtgc aatagctgtt cttgagtact
    13201 tgaaaaaaac aaacatcaag atcggattgg gatcagcaag caaaaatgca cgcctcattt
    13261 tagaaaaaac gaatttgaca agttattttg atgtccttat tgacggcacg caagtatcaa
    13321 aagcaaaacc tgatccagaa gtttttctca aaggagcaca gcaattgaat gtaccgccaa
    13381 atgcatgctt ggtcattgaa gattcagaag ctggttgtca agcagctttg gctggtaaca
    13441 tgcatgtatt gggaataggt gaaaacataa atcttccttc tgctgaatat gtgattcctg
    13501 atttaaccgt ttttgatcaa gtacgttcgt tttggcattt atcagaagcc gtatcacgta
    13561 tataatagtt tccgtttttt ctgtcacaag caaaacgtta tcctcctttt ggaaatcaag
    13621 gtatgatgag aaaaaagggg gattttatat gacaaaaaaa gcagttttcg caggcggatg
    13681 cttttggtgt atggtcaaac catttgaaga gcagccaggg atcatttctg tgacatccgg
    13741 ttatactggc gggcatgtac caaatccaac ttatgaacaa gtaacaacag gcacaacagg
    13801 gcatacagaa gcagtagaga tcgaatatga tccagaagtt atttcttatg atcagttggt
    13861 ggaaatctat tggcagcaaa ccgatccgac tgacgcatta ggacagttcg ctgatcgcgg
    13921 tgattcttat cgtccagtga tctactataa tgatgaagaa gaacgaaata ttgcagagcg
    13981 ttcaaaacaa gcattgcaag acagcgggcg attcgataag ccaatcgtga caaaaatcga
    14041 accaagaagc gaattttatc ctgcagaaga ttaccatcag gattattaca agaaaaacaa
    14101 gctccattac aatctttatc gagaaggttc tggacgtgca ggtttccttc gtcgacattg
    14161 gggcgtcaag aaataagaag ccgaaaaagc tacttatgga acaccgtata tcagaggttg
    14221 taaaaccagc attttgagct aatactgagg gtcagctgat tgaacaccat ttagtggaga
    14281 ggttgtgaac ctgtcgccta gctccaagtc acaaggattt ggtaagagaa cactatatat
    14341 cagaggttgt gacaagtcgt ttgtcacaac cttttttcaa tacctaagat gaaggacaat
    14401 cagcaacgca ctgctcaaaa aaggaatgaa aggcagcttt tccaactttt ttttatgaca
    14461 gataaagtaa acagaaaaaa cggatattcc aaacagacta gcaagaaaca agatctgaag
    14521 caactcttct agagacaagc caccactcca agaaagcatc aacagagtat cgccagctcc
    14581 gagataggat tgaccatagc gaaggcacac accgcctacg ataaacgtta acagcacact
    14641 ttgccattgg aagacgccgg cctgcaacca aaaaatccat aaaattgccc aagaacagaa
    14701 tagcgtgtga gaatggacca tgaagtaaaa taaatccatc aacgaaaata gcaaagccga
    14761 aagcatccat atgaacgtcg aaaaatttct tgcggaagaa aaagtaaaga accaggcaaa
    14821 aagaccacca cataaggctt ctgccaacaa atagtagaag ggcaaccgac acctgcaata
    14881 gcggcagcga aatcgttgga cgatgatcga gagaatcgga atcaattcat accaagaaag
    14941 cgaacgacag catttgacac aatgagatct aggataaata atagagtgac ctaacggaat
    15001 ccgctgggcg gttaaacata aaaatgatcc gatacagcaa ccaatgataa aataaagtaa
    15061 cacgccatct cccccttttc aaaaccaaga tacgcaagct taaactaaat ttttgtacat
    15121 acataaaatt tgaggtgaaa aaatgaacgt ttcaatcgga ttagatatcg gaaccacaca
    15181 aaccaaagca gttgctttca cagatgaagc ccaagaagca gcatccgctt attttcgcta
    15241 ccctttgatc caagaaacac aagggatggc agaacaagat caggaattga tctttcaagg
    15301 tgtctgcacc gttataaaag aagtaattaa acagttgcct tcagacacaa tcattcaatt
    15361 tgtctccttc tcctctgcga tgcatggcct gatattgttg gatgaagaga aaacaccgtt
    15421 atctcgaatg atcacttggg cagataaccg agcagcggac gaagcagaaa aactgaaata
    15481 taccgtgctt ggcaaagaat tttatcggca gactgggacg ccgattcatc caatgacacc
    15541 attgaacaaa atcaagtggc tgcaaaacag ccaacctgaa ttattcaaaa aaactgctta
    15601 ttttgtcggt atcaaagact atatctttta tagattgttt ggcgaactga tcagcgacta
    15661 cggaacagct tcaggaactg gatattttga tatccatgaa ttccaatggg cagaagaagt
    15721 gcttgactac ttgaacattc gaatagagca attgccgcaa gtctttcctt ccacctatca
    15781 agtaacaggt ctaccatcaa aaacagcagc agaactaggt ttgccaaatg atatatcatt
    15841 tgttttggga ggggcagacg ggccgctttc taatctaggg ttaggtgctt ttggtgatac
    15901 gatcgccgct ttgaccgtag gaacgagcgg agcacttcgt tttattacta ctcagcctca
    15961 gttccatcca gaaatggaga cgttttgtta tgttctggat cagaaacatt gggtagtggg
    16021 aggagcgact agcaatggtg ctggcatctt tgattgggca gggcatacat ttttacagga
    16081 agtcgttcaa tctgctaaag aaaaaggaga aaatccatat gatgcactgc tttcggctat
    16141 cgcttttgtg ccagcgggtg ccaatggtct gatttttcat ccctatttac ttggtgagcg
    16201 agcaccgctt tgggacgcag atgcgtttgg cagttttatc ggtttacagc ggcaacatac
    16261 agaaaaagaa atgatgaagg cgattctaga agggatctgt ctcaaccttt atcggatttt
    16321 aaaaggagtt tgccaggaaa atcagctcac agagatccgt gctacaggcg ggtttgctca
    16381 atctccgatt tttcgtcaga tgatggctga tgtactgggt tacccgcttg ttttccctac
    16441 tgtcacagaa gcatccgcat taggagctgt gattttaggc tggagaagca tcggaaagat
    16501 ccaaagtatc agtgaagcac aggaattgat agaattgaaa gagcaagttg gagtacaaga
    16561 aactgatcat aaaaaatatc agaaaatcta tccattattt gtttcgacac aaaagcattt
    16621 agcacagact tatcagcaat ttgctcaact gcgagaagaa ttagctgatt ctgaaaaatg
    16681 attggcttta cggataaaaa agcgaactga aaatcgttct caggtaaatg attttgttgt
    16741 acttttcatt gaaagtctac tattatttta agtgtaagaa aatgttagga ggcaagaaag
    16801 atgaaattcc aacaaaccaa agaagtatta aatcaattag ttgcagatct aagccaaatg
    16861 tctgttgttg ttcaccaaac acattggtac atgagaggac caggtttcct tacattgcac
    16921 ccaatgatgg atgagttcat ggatgatttg aacgctcagt tagatgaagt atccgaacgc
    16981 ttgatcacat tagacggttc accttattct acattgcgtg aatttgcaga taacacaaaa
    17041 atccctgatg aaattggccg ctgggatcgt actattccag aacgtttgga aacacttgtt
    17101 gcaggctatc gttatctagc agatctttac caaaaaggaa tcgaagtttc tggagaagaa
    17161 ggcgacgatc caacacaaga tatctttatc gaattcaaaa cagccacaga aaaacgtatc
    17221 tggatgatcc aagctcattt gggcaaagcg ccagagatcg acgcgtaaaa actgttgttg
    17281 aacttgaccg aaaaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa
    17341 cgtcaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt
    17401 gaacaccgtt taccaagagg ttgtaagcct gccgttcagc tccgagtcac aagaaacaat
    17461 tttaaaaaac agcttcttat gttttatgga tattgtgact tgtgaccgag gagttggcag
    17521 gagaacaccg tttattcaaa ataaggggtt gtgacaagac ttttgtcaca gcctcttttt
    17581 gtttttcatt ttgacaggaa acgacccttt tatataaaag aaagaaaaga agtaaagttt
    17641 catataaatt tttcaatcaa gcagaaaaac gttgacattg actagattag cgggtaatat
    17701 gttaaatggt attgtttgcc ccacaaagag ccccggaaac tcgaagtgta tgtcaaacat
    17761 cagaacttaa attggaggaa aataacgtgc gtacaacata tatggccaaa ccaggcgaag
    17821 tagaacgtaa atggtatgta gtagacgcaa ctgatgttcc attgggacgt ctttcaacag
    17881 ttgttgcatc tgtactacgt ggtaaaaata aaccaacttt cacacctcat ttggatactg
    17941 gcgatttcgt catcgtcatc aatgcagaca aagtgaaatt aacaggtaaa aaagcgactg
    18001 acaaaattta ttaccgtcac agcaactacc caggaggatt gaaatcaatc actgctggcg
    18061 aattgcgtgc taaaaattct cgtcgtttga tcgaaacttc tgtaaaaggt atgcttccta
    18121 aaaacacttt aggccgcaaa caatttacaa aattgaacgt atacggtgga gcagaacatc
    18181 cacatgcagc acaacaacca gaagttttag acatcacaaa cttaatctaa taggagggaa
    18241 tttcattggc acaagttcaa tatatcggca caggccgtcg taaaaacgca gttgctcgcg
    18301 tacgtctagt accaggaact ggcaaaatta ctgtaaataa aaaagatgtt gaagaataca
    18361 ttccacatgc tgacttgcgt gaagtaatca atcaaccttt cgctgttact gaaacaaaag
    18421 gcgcatatga cgtatttgtt aacgtaaacg gcggaggata cgctggacaa tcaggagcta
    18481 tccgtcatgg tatcgctcgt gcattgctag aagttgatcc tgacttccgt ttagctctta
    18541 aacgtgctgg cttacttact cgtgacgcac gtatggtcga acgtaaaaaa ccaggtctta
    18601 agaaagcacg taaagcttca caattctcaa aacgttaata tcctttatat atcaacgttt
    18661 ctggcacttt tcaattattt ggaaagtgct tcttttgttt ttaagaacac atttaagaac
    18721 acatatcaat cacacttatt caatcctctc tgaaaagctt caaaaacagc cttatcaact
    18781 atatctggtg tgttcacata tatctttgtt gtagaaatat ctgagtgtgt taaagcattt
    18841 gaaatttgat tcatatctgc tccaccttgt ctagctatag ttgcataagt atgacggagt
    18901 ttgtgtggag ataaatgaac taagtcagga tgccttcttt taattgaatt gattcgatag
    18961 tttaggtaat cggcatgaac aggacaattt acttctccag atggtttagt ataagtaaag
    19021 agatactgat ctaacgtttg cttaattttt aatttcaaca attgatcagc ttgtaccgat
    19081 ttccattcac ttagcaatgt cattacaact tctggtactt gaatgacggt atcttttcga
    19141 ccttttgtaa acttttttct ttgatattta tctaaagtat gtttaagacg gaccgtttgt
    19201 ttttcaaaat caatatattt ccattgcaaa gcgtaagtct cactttttct atcaccaaga
    19261 tacagggtaa tcataaaaag tgtgtaatca tgaaaagtaa gtaaatgatt attaaaatca
    19321 tcatttactg cttctatcca attagtaagt tcttttgatg atagtgcttg cttaccttct
    19381 tgtattcttt tttcttctaa agcatttttt ttaggtgcag tgatactttg aataatttta
    19441 gtagttcgat taaattcgat ataatttagt atttctgcaa tatcgaacat ggaacgaaca
    19501 tatcctttta tgatttttat atttgcatat tctttcgatt ttttggttaa ttcatctgat
    19561 attatctcag tattg
//
LOCUS       DAZULG010000052        19506 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000052 DAZULG010000000
VERSION     DAZULG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.053"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG9CY_001599"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG9CY_001599"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS4938111.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACG9CY_001600"
     CDS             complement(1172..1867)
                     /locus_tag="ACG9CY_001600"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS4938112.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACG9CY_001601"
     CDS             complement(1880..2596)
                     /locus_tag="ACG9CY_001601"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS4938113.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="ACG9CY_001602"
     CDS             2926..3531
                     /locus_tag="ACG9CY_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS4938114.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="ACG9CY_001603"
     CDS             3856..4179
                     /locus_tag="ACG9CY_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS4938115.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="ACG9CY_001604"
     CDS             4361..5731
                     /locus_tag="ACG9CY_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS4938116.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="ACG9CY_001605"
     CDS             complement(5861..7162)
                     /locus_tag="ACG9CY_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4938117.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9383)
                     /locus_tag="ACG9CY_001606"
     CDS             complement(7392..9383)
                     /locus_tag="ACG9CY_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS4938118.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDKVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="ACG9CY_001607"
     CDS             complement(9527..9793)
                     /locus_tag="ACG9CY_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4938119.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="ACG9CY_001608"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="ACG9CY_001608"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS4938120.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="ACG9CY_001609"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="ACG9CY_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS4938121.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /gene="def"
                     /locus_tag="ACG9CY_001610"
     CDS             13137..13700
                     /gene="def"
                     /locus_tag="ACG9CY_001610"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4938122.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="ACG9CY_001611"
     CDS             complement(13953..14474)
                     /locus_tag="ACG9CY_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS4938123.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="ACG9CY_001612"
     CDS             complement(14477..15319)
                     /locus_tag="ACG9CY_001612"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS4938124.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="ACG9CY_001613"
     CDS             complement(15312..16037)
                     /locus_tag="ACG9CY_001613"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS4938125.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="ACG9CY_001614"
     CDS             16213..16509
                     /locus_tag="ACG9CY_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938126.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16765..16939
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17077..18669
                     /locus_tag="ACG9CY_001615"
     CDS             17077..18669
                     /locus_tag="ACG9CY_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4938127.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="ACG9CY_001616"
     CDS             18682..19422
                     /locus_tag="ACG9CY_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4938128.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5579 a         4001 c         3702 g         6224 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttt atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaa
//
LOCUS       DAZULG010000053        19461 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000053 DAZULG010000000
VERSION     DAZULG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.054"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..251)
                     /locus_tag="ACG9CY_001617"
     CDS             complement(87..251)
                     /locus_tag="ACG9CY_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938129.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            546..1874
                     /locus_tag="ACG9CY_001618"
     CDS             546..1874
                     /locus_tag="ACG9CY_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS4938130.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2076..2909
                     /locus_tag="ACG9CY_001619"
     CDS             2076..2909
                     /locus_tag="ACG9CY_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS4938131.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2925..3662
                     /locus_tag="ACG9CY_001620"
     CDS             2925..3662
                     /locus_tag="ACG9CY_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS4938132.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3817..4785)
                     /locus_tag="ACG9CY_001621"
     CDS             complement(3817..4785)
                     /locus_tag="ACG9CY_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS4938133.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5017..5850
                     /locus_tag="ACG9CY_001622"
     CDS             5017..5850
                     /locus_tag="ACG9CY_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS4938134.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5884..6723
                     /locus_tag="ACG9CY_001623"
     CDS             5884..6723
                     /locus_tag="ACG9CY_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4938135.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6749..7309
                     /locus_tag="ACG9CY_001624"
     CDS             6749..7309
                     /locus_tag="ACG9CY_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4938136.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7508..9229
                     /locus_tag="ACG9CY_001625"
     CDS             7508..9229
                     /locus_tag="ACG9CY_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS4938137.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9394..10536
                     /locus_tag="ACG9CY_001626"
     CDS             9394..10536
                     /locus_tag="ACG9CY_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4938138.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10552..11763
                     /gene="thiI"
                     /locus_tag="ACG9CY_001627"
     CDS             10552..11763
                     /gene="thiI"
                     /locus_tag="ACG9CY_001627"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS4938139.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12336..12983
                     /locus_tag="ACG9CY_001628"
     CDS             12336..12983
                     /locus_tag="ACG9CY_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4938140.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13112..13328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13376..16021
                     /locus_tag="ACG9CY_001629"
     CDS             13376..16021
                     /locus_tag="ACG9CY_001629"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS4938141.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16331..17644
                     /locus_tag="ACG9CY_001630"
     CDS             16331..17644
                     /locus_tag="ACG9CY_001630"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS4938142.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17634..18287
                     /locus_tag="ACG9CY_001631"
     CDS             17634..18287
                     /locus_tag="ACG9CY_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4938143.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18342..19034
                     /gene="radC"
                     /locus_tag="ACG9CY_001632"
     CDS             18342..19034
                     /gene="radC"
                     /locus_tag="ACG9CY_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS4938144.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19184..19384
                     /locus_tag="ACG9CY_001633"
     CDS             19184..19384
                     /locus_tag="ACG9CY_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4938145.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         6555 a         3284 c         4125 g         5497 t
ORIGIN      
        1 tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac
       61 gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa
      121 taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc
      181 ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc
      241 aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt
      301 attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc
      361 attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac
      421 atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga
      481 gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg
      541 attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa
      601 atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg
      661 aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga
      721 aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc
      781 gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt
      841 ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa
      901 aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg
      961 gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga
     1021 tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac
     1081 tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta
     1141 ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag
     1201 gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg
     1261 accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt
     1321 atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag
     1381 agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa
     1441 ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg
     1501 atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg
     1561 atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa
     1621 gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa
     1681 aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga
     1741 gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga
     1801 aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg
     1861 gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg
     1921 cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta
     1981 taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac
     2041 gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg
     2101 atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc
     2161 gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat
     2221 tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata
     2281 cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg
     2341 gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc
     2401 cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg
     2461 aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag
     2521 aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg
     2581 tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga
     2641 gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt
     2701 atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat
     2761 ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga
     2821 tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc
     2881 aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata
     2941 ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt
     3001 cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca
     3061 gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta
     3121 atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata
     3181 agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa
     3241 tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag
     3301 agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg
     3361 aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta
     3421 atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact
     3481 ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg
     3541 ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac
     3601 atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat
     3661 aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat aagaagaaat ttacgaaaat
     3721 tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag
     3781 cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa
     3841 ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact
     3901 attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata
     3961 atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt
     4021 ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa
     4081 agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg
     4141 catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg
     4201 ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg
     4261 acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg
     4321 tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca
     4381 acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact
     4441 tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa
     4501 aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga
     4561 tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg
     4621 tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc
     4681 tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt
     4741 catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt
     4801 acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat
     4861 cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa
     4921 aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa
     4981 agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg
     5041 tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg
     5101 tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt
     5161 gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga
     5221 atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat
     5281 tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa
     5341 ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag
     5401 tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa
     5461 tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat
     5521 gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt
     5581 gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg
     5641 cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa
     5701 gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt
     5761 ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga
     5821 ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag
     5881 tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa
     5941 gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt
     6001 gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat
     6061 atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac
     6121 ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa
     6181 atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg
     6241 atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa
     6301 aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata
     6361 ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta
     6421 gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc
     6481 ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa
     6541 atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta
     6601 ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg
     6661 actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat
     6721 tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga
     6781 aacattccac aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa
     6841 tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac
     6901 ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa
     6961 agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt
     7021 agatgcactg acagatatcc tttactttac ttatggttct ttttcattac taggaatagt
     7081 gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg
     7141 aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga
     7201 atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc
     7261 taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca
     7321 ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg
     7381 catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt
     7441 ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga
     7501 accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat
     7561 tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt
     7621 agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa
     7681 aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa
     7741 tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct
     7801 caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat
     7861 ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac
     7921 ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga
     7981 aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat
     8041 caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt
     8101 tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat
     8161 gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga
     8221 aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct
     8281 tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa
     8341 aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt
     8401 atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac
     8461 gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga
     8521 tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca
     8581 aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca
     8641 cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga
     8701 tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa
     8761 agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt
     8821 agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac
     8881 tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt
     8941 caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga
     9001 tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca
     9061 tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata
     9121 tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc
     9181 tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt
     9241 tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt
     9301 gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata
     9361 atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca
     9421 accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg
     9481 aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga
     9541 aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga
     9601 acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat
     9661 catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa
     9721 gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac
     9781 gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat
     9841 gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa
     9901 attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg
     9961 acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc acggggaact
    10021 ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa
    10081 gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct
    10141 ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta
    10201 gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac
    10261 tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat
    10321 gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat
    10381 atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt
    10441 cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc
    10501 aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac
    10561 agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt
    10621 atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc
    10681 catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt
    10741 ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa
    10801 gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa
    10861 gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa
    10921 ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg
    10981 cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat
    11041 gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg
    11101 ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa
    11161 attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca
    11221 aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca
    11281 tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt
    11341 actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc
    11401 attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct
    11461 atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa
    11521 atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat
    11581 tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat
    11641 caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag
    11701 atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta
    11761 taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag
    11821 ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta
    11881 tcaagaagtt atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag
    11941 agatcaagct acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt
    12001 tgtgacagaa gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa
    12061 tttttgtgaa atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga
    12121 ggttgcgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
    12181 atattttacg tttggttgag cttattaccg cagatgcaag cttttgaaca ccgttttatt
    12241 tgtctcacac ccttttcctt cctacttgta accttgctcc cgtgttagaa acatgttaca
    12301 ctaaatatgt gaaattatta acaaggagcg tcgttatgcg agaaaaaaca gatacactgc
    12361 caaaagcaac tgcgaaaaga ttacctttat atttacgcta cttgaagatg ctggatgact
    12421 caggtataag tagaatcaaa tcaaatgagt tcagcgaaat cacccaaatt ccttctgcaa
    12481 cgatccgcag agacttctcg caattaggcg agctaggcag aagcggatat ggctatgatg
    12541 tcccttttct tatcgatgta ttcaataata tccttaatac taaagaagaa aaacgaatcg
    12601 cattagtagg atatggaaat cttggcaaag cattgaaaca caacaatttt agaagaaatg
    12661 aaaatctaaa tatcgtgtgc gtctttgata atgatccagc actgatcaat cgagtaattg
    12721 atggagaaat gatctatccg attgatcgtt tcgcggagat agccaaagca aaaaatgtga
    12781 cagtagcaat ctctacggta ccaagtaaat attcacaatc tgctattgat gaaattgtca
    12841 aaggaaatgt aaccgctata cttaattttg cgcctgacag agtaactgtc ccagcttatg
    12901 tcaatgttca atacattgat ttgacaaccg agttgcagac gcttatttac tttgatgaga
    12961 attactcaga agttttttct taggaaaatt tgagtaggct tattgatctt gcgagaactc
    13021 gtcgtaattc tttcaaaaat tttttagtac taaatgaagt ggttttttca cgaccacttg
    13081 ttgactaaaa aaaaaaacca ttgtagaatg aagcataacg ctgaacaaga ggagtaactg
    13141 gcacattcat tcttagagag agaactatct gctgaaaggt tctcaatgac aaccagtgaa
    13201 ggtagcttgg gagtttctgt cttgaatgga gtagagatag acgagtcgtg atcgttaact
    13261 cacttgagag gcaataatga ttgattattg caatttaggt ggtaccgcgt aaatcatacg
    13321 tcctagaata cgaaagtatt ttaggacgtt ttttagttta aaggaggaaa acagcatgac
    13381 ggaagaaaat aacctatcaa caaaatataa tccgcaagaa gttgaagctg gacgttatca
    13441 gcattggtta gaacaagatt tgtttaaacc gagcggagat aaaaaagcaa aaccttattc
    13501 aatcgtaatc ccgcccccaa atgttactgg aaaacttcat ttagggcatg cttgggatac
    13561 gaccttgcaa gatatgctga ttcgtcaaaa gagaatgcaa ggatatgata ctttatggct
    13621 gcctggtatg gaccatgcag gaatcgcgac acaagcaaaa gtagaagcga agcttagaga
    13681 gcaagggatt tctcgttatg atctaggtcg tgaaaagttc atcgatcaag tgtgggaatg
    13741 gaaagaagag tatgcgagcc acatccgcga acagtgggct aaaatgggct tatctttaga
    13801 ttacagtagg gaacgtttta cattagatga tggattatct gaagcagtac gtaaagtttt
    13861 tgttacctta tacgaaaaag gtttgattta tcgtggtgaa tatatcatca actgggatcc
    13921 tcaagcacga acagctctat ctgatatcga agttattcat aaagatatcg aaggcgcctt
    13981 ttatcatatg agctatgaac tagcagatgg atcaggcgta gtagaaattg cgacaacccg
    14041 tccagaaacg atgttgggag atacggcgat tgctgttcat ccagaagacg agcgttatca
    14101 agcgctcatc ggcaaaaaag tgattctacc tttagtcaat aaacaaatcc caatcattgc
    14161 agatgaatat gtagatatgg aattcgggac aggtgtagta aaaatcactc cagcacatga
    14221 tccaaacgac tttgaggtag gtaatcgtca tgatcttcca cgcgtaaatg ttatggatga
    14281 tgatggtacg atgaatgaac tagcaggaaa atatgctgga atggaacgtt ttgctgctcg
    14341 taaagcaatc gtgcaagatc tgaaagaaat cggtcgattg attaaaatcg atacgatgat
    14401 ccacagtgtg ggtcattccg agcggacagg tgtcgttgtt gaacctcggc tgtctactca
    14461 atggtttgta aaaatggcgc cgttagcgaa aaaagccatt gataaccaag aaacagatca
    14521 agcagtggaa ttcttccctc caagattcaa tcaaacgttt ttacgttgga tggaaaatgt
    14581 ccatgattgg gtaatctcac gacaattatg gtggggacat caaattcctg catggtatca
    14641 caaagaaaca ggtgaaatgt atgtagggat ggaagcacca gcggatagtg aaaactgggt
    14701 acaagatcct gatgttttag acacttggtt tagttcagct ttgtggcctt tctcaacaat
    14761 gggctggcca gatacggaaa gcgaagatta taagcgatac ttccctacta gcactttagt
    14821 aaccggttat gacatcattt tcttctgggt aagcagaatg attttccaaa gcttggaatt
    14881 tactggcgaa cgtccatttg agaatgtctt gattcatgga ttgattcgtg acgaacaagg
    14941 gcggaagatg agtaaatcat taggtaatgg aatcgatccg atggaagtaa tcgaaaagta
    15001 tggagcagat gctctccgct ggttcttatc gaatggttct gcaccaggac aagatgtccg
    15061 cttcagctat gaaaaaatgg atgcttcttg gaactttatc aacaaaatct ggaatgcttc
    15121 acgtttcgtt atcatgaata tcgaaggaat gaccgtagaa gagatcgatt tatctggaga
    15181 aaaatcagtg gctgaccggt ggatccttac tcgtttgaat gaaacgatcg aacgagtaac
    15241 tgagctattt gatcgttttg aatttggtga agcaggtcga caactgtata actttatctg
    15301 ggatgatttt tgtgactggt acatcgaaat gagtaaagaa gtactttatg gagatgatgc
    15361 tgtttcaaaa caaaccaccc gtagcatctt agtccataca cttgatcaga tcctacgttt
    15421 attgcatcca atcatgccat tcgttacaga agaaatatgg gaacatattc ctcatcaggg
    15481 aaattctcta gtagtggcag aatatcctgt tgtacgtcca gagtttgatg atgaaacagc
    15541 ttctaaaggg atggaagtat tgaaagaatt gattcgagcc gttcgaaata ttcgttcaga
    15601 agtgaataca ccattgtcta aaccaattac gttgctaatt aaaacaaacg atgctaaaat
    15661 cgatcaattt ttagttgaaa atacaagcta tatcgaacgc ttctgtaatc ccgaagagtt
    15721 gacgatatca agtgagatca ctgcgccaga tctagcgatg tcagctgttc tgacaggtgc
    15781 agaaatcttc ttgccgttag caggattgat caatattgaa gaagaaatca aacgattaga
    15841 aaaagaactg gaaaagtgga caagcgaagt aaaacgtgtc caaggaaaac tttctaatga
    15901 acgttttgtt tctaacgcgc cggaagaagt agtagaggca gagcgtgcaa aagaaaaaga
    15961 ttatttagag aagcaaacgg ctgtcaaaga acgaatcgaa agtttgcgta ctattcaata
    16021 agaatagaaa aagagagtgt gaaatcagct agctattact aaaagctagc tgattgaaca
    16081 tcgttacagg aaagagtttg agcctcactc ttagcttcga ttcataagga gatgtgacat
    16141 aaattttgtc gcatctcttt tttagtctac tcttattatg taggagaagg agggaggtgg
    16201 atcatgtatc aagagtcaat aaagtagttg ataaaatcgt tcctctctct gtttcttttc
    16261 tctttgtcat acttttattt ttcttatgtg ttctatttgt tataataaaa gagaaatggg
    16321 ggaattgttg atgacgattg aagaagcaat agaatggatc catagtcgat taccatttgg
    16381 ttcacgtcct ggccttgacc gagtcgaagc tttattagaa gtggtagggc atccagagag
    16441 aaaagtaaaa acgatccata tcgctggaac aaacggaaaa ggttctactg tagcttattt
    16501 gagaagccta ttagaagagg ctgggctgac agtaggtaca tttacttcac cttatatcga
    16561 aacgttcaat gaacggatcg caatcaatgg caaaagcatt ccagacaatg atttgatcca
    16621 gcttgtaaaa aaatatcagc cactgatcaa acaacttgat caatttgaag cagtagctgg
    16681 tattactgaa ttcgagacat taacagcaat ggcatttgat tattttttga tgaagaaagt
    16741 ggacgtagcg attatcgaag ttggcttagg cggcttattg gattctacca atgtagctgt
    16801 accaatgctg acgggaataa caacgattgg acttgatcat accgaaatac ttggagatac
    16861 aattgaagaa atcgctgcac aaaaagctgg aatcatcaaa caaggcgttc cagtagtaac
    16921 aggaaatatt gccccagaag cttttactgt catccaacaa acagctaaag caaaaaatgc
    16981 accgctttat gtatacgggg aaaattataa aataaactat ctgcatcctg atgagatgtg
    17041 gggagaagta tttgaatttt ttaatgaaat aggaaagatc cctcaattaa ccactccgct
    17101 tttagggcaa catcaagctg aaaatgcagg agtagccatc gaattgtatt ttgtttactg
    17161 tcaattggaa aagctgcctt tccgagaaaa agatgttttg aagggattga agaaatctcg
    17221 atggccagga agaatggagc gtttgagtaa cgagccatta gttgttttag atggtgccca
    17281 taatacgcat gcaatgaacc ggcttgttga gaatttgaaa aaagaattca aaggatatca
    17341 aatcaacatt ttgttttccg ccttacgaac taaaaatgtt agagaaatgc tagaacgatt
    17401 gcagcaagta ccaaatgtcc atatttattt gacgaccttt gaacatccaa aagccatcga
    17461 attaagtgaa gtatcagatt tagcaagtga gagagtatca atcgtttcat tgtggcaatt
    17521 cggattagca gatatattgg aaaagatgac aaatgaagac gtattagttg tgaccggttc
    17581 actttatttc atttcagaag ttcgccattt attaatacaa ttaggaggag atcatgaagt
    17641 ataatgcagt gatttttgac atggatggtt tgctgttcga cacagagtta gtgtattacg
    17701 aagccacaca agtagtagca gacaagatgg gttttcctta tgataaagac ctgtatttaa
    17761 aattcttagg tgtttcagat gaagaagtat gggcgaatta tcatatgatt ttctcggaat
    17821 ttggcaaaac aagtgtacaa agattcatag atgattcata ccaggaaacg cttgaacgtt
    17881 tttcgcaagg agcggtacaa atgaagcccg gtgtgttgga actcctctct tttttagaag
    17941 aacatcagat tcccaaagtt gtcgcttcca gtaaccaacg agcagtcatt gaacttttat
    18001 tgaagaagaa caatctgttt gaacgatttg agacgattgt ttctgcagaa aatgttcaac
    18061 gagcaaaacc agacccagaa attttcttgt tagctaatca gaagctaggt acagcgaaga
    18121 aagaaacatt gattttagaa gattcacaaa atggtatttt agcagcttct gcggctgaga
    18181 tcccggtagt catggtacca gatctgcttc caccatccca agaattgatc aaaaaaacag
    18241 ctggggttgt ttcttcttta catgaaatcc ctgcattgtt aaaataaaaa tccatctctt
    18301 ttcgtatctt attttagata agatagaaag gagatgtttt tttgggaaaa cgattgatca
    18361 aagaagtacc tactagctct ttaccaaggg aacgtatgga aatttatggc gcggaagctt
    18421 tatcggatca ggagcttttg gcaatcttac tgcgtaccgg tcaacaccct tatagtgtga
    18481 tgtcgattgc tggaaatctg ttaaaaacgt ttggaggact ggcctcttta cgacaggcta
    18541 ctttgcatga gttagaagag attcgaggga taggacgcgt gaaagcaatc gaaatcaaag
    18601 ccttgatcga attaggtcga cgaattcaga cggatcatga aagagcggct ccaccggttc
    18661 gtaccagcta cgaattggcc cagcaattga ttctggaaat gaaagatcac aaacaagaac
    18721 atttggtttg tatttatttg gacacgaaga atcaagtgat cttaaaaaaa acagtgttca
    18781 tcggttcgct gaatcagtca gtcgctcatc ctagagaaat tttccattat gctgtacgtt
    18841 attgtgcagc aaggatcgtt ttagcgcata atcatccttc tggtaatgtt attccatcgc
    18901 agcaggatat gaattttacc aaaaggatac aaaagtgtgg agaaatgatg gggatcactg
    18961 tactggacca cttgatcatc ggacgcaaac gatattttag tttgagagaa gaaggaatga
    19021 tggaagagaa gtaagttgct ttcttgcaaa aaaaatgaag caagtgtaca attatattgt
    19081 aattttgttt gggaatagaa gttgaaacat ctgttgcacc tctatcgtac acgagcaatg
    19141 ttacaattta aattagtgct tttggcacga ggaggatatt attatggaaa acggaacagt
    19201 aaaatggttc aacgctgaaa aaggatttgg atttatctca cgtgaagatg gaagcgacgt
    19261 attcgttcac ttctcagcta tccaaggtga cggattcaaa acattagaag aaggtcaagc
    19321 agtgactttc gacgttgaag aatcagaccg cggcccacaa gctgcaaacg ttgtaaaagc
    19381 ttaattttat ctgtaaatca aattgatggt agacatcaat ttttacgaga gaacaaagcg
    19441 agtaacaaaa atacctgcca c
//
LOCUS       DAZULG010000054        19248 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000054 DAZULG010000000
VERSION     DAZULG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.055"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..283)
                     /locus_tag="ACG9CY_001634"
     CDS             complement(<1..283)
                     /locus_tag="ACG9CY_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS4938146.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNT"
     gene            complement(309..740)
                     /gene="ruvX"
                     /locus_tag="ACG9CY_001635"
     CDS             complement(309..740)
                     /gene="ruvX"
                     /locus_tag="ACG9CY_001635"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS4938147.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(737..1003)
                     /locus_tag="ACG9CY_001636"
     CDS             complement(737..1003)
                     /locus_tag="ACG9CY_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS4938148.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1257..1982)
                     /locus_tag="ACG9CY_001637"
     CDS             complement(1257..1982)
                     /locus_tag="ACG9CY_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4938149.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2348..2418)
                     /locus_tag="ACG9CY_001638"
     tRNA            complement(2348..2418)
                     /locus_tag="ACG9CY_001638"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2384..2386),aa:Gly,seq:ccc)
     gene            2535..3287
                     /locus_tag="ACG9CY_001639"
     CDS             2535..3287
                     /locus_tag="ACG9CY_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4938150.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3303..3782
                     /locus_tag="ACG9CY_001640"
     CDS             3303..3782
                     /locus_tag="ACG9CY_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4938151.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4033..5613)
                     /locus_tag="ACG9CY_001641"
     CDS             complement(4033..5613)
                     /locus_tag="ACG9CY_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS4938152.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5606..6349)
                     /locus_tag="ACG9CY_001642"
     CDS             complement(5606..6349)
                     /locus_tag="ACG9CY_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938153.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6525..7301)
                     /locus_tag="ACG9CY_001643"
     CDS             complement(6525..7301)
                     /locus_tag="ACG9CY_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4938154.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7549..8385)
                     /locus_tag="ACG9CY_001644"
     CDS             complement(7549..8385)
                     /locus_tag="ACG9CY_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS4938155.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8396..9325)
                     /locus_tag="ACG9CY_001645"
     CDS             complement(8396..9325)
                     /locus_tag="ACG9CY_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS4938156.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9517..9870
                     /locus_tag="ACG9CY_001646"
     CDS             9517..9870
                     /locus_tag="ACG9CY_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4938157.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(9944..10600)
                     /locus_tag="ACG9CY_001647"
     CDS             complement(9944..10600)
                     /locus_tag="ACG9CY_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4938158.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10826..12760
                     /locus_tag="ACG9CY_001648"
     CDS             10826..12760
                     /locus_tag="ACG9CY_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4938159.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12846..14069)
                     /locus_tag="ACG9CY_001649"
     CDS             complement(12846..14069)
                     /locus_tag="ACG9CY_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4938160.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14063..15934)
                     /locus_tag="ACG9CY_001650"
     CDS             complement(14063..15934)
                     /locus_tag="ACG9CY_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS4938161.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(15955..17073)
                     /locus_tag="ACG9CY_001651"
     CDS             complement(15955..17073)
                     /locus_tag="ACG9CY_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4938162.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17156..17989)
                     /locus_tag="ACG9CY_001652"
     CDS             complement(17156..17989)
                     /locus_tag="ACG9CY_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4938163.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(17982..18899)
                     /locus_tag="ACG9CY_001653"
     CDS             complement(17982..18899)
                     /locus_tag="ACG9CY_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4938164.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19126..>19248)
                     /locus_tag="ACG9CY_001654"
     CDS             complement(19126..>19248)
                     /locus_tag="ACG9CY_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4938165.1"
                     /translation="QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         5983 a         3843 c         3210 g         6212 t
ORIGIN      
        1 atgtattgaa tacttcttcg atcatatccc attctgcgtc tgtttcgatt tgttctaact
       61 cgccttctgt tccatcttcg ttttcgatgt atgcataagc ttgtaattct acatcttcat
      121 cttctgaaac accagctgga tataaaagaa catagttacg tccaaattct tcttgtccgt
      181 caactgttag caagatttcg tataatgttt catttccttg gtcatccacc aatgtgatgt
      241 gttcgtgtcc ttcgtgttcg tgggaatggt tatgttctgt cataagatta cctcttttct
      301 ttttattatt aaaataattg cttacctgag ccgtctaaat aattttgtaa aatcatcaca
      361 gctgctaatt tatcgatgac tttttttcgt ttcgctcttg aagtatttgc ctgctctact
      421 agcatacgtt ctgcctgaac agtggttagt cgttcatctt gataatcaac cggcaaggag
      481 aatagttctt tgatcttctc tccatatgac atggaagctt ctgcccttgg tccgatggaa
      541 ttgttcatat tcttaggtaa tccaactaca aaacggtcta cttcgtactg cgccactaat
      601 tccttcaacc gatcaaaacc aaactcttct tctgcctcat tgattcgaat gatttctact
      661 ccttgagctg tccagccaaa gggatcgctc acagcaacgc ctactgtctt agaaccgaca
      721 tccaagccca ttactctcat aaattcactc catgatttga gaggtagtct ttgacaatta
      781 cttccatgat ttcatcgcgt tcgtgacggc gaatcaaatt tcgtgcatca cgataacgag
      841 gaatgtaggc cgggtctcca gaaagcaagt agccgacaat ctgattgatt ggattgtagc
      901 ctttttcttc caaagcccga taaacaatcg ctaatgtttc gctcacttct tttttgcgac
      961 tatcgtcaaa accaaaacgt acggtttcat ctgtaaaacc catgattcta cacctctttc
     1021 ccgcaaggta attccagctt accttctaca cccttatttt actcgaagaa agggaaaact
     1081 acaaacaaat attccaaact tctgaataaa ttaagaaaca cgatttgtct gatttttcct
     1141 tttttctgtg gctttttcaa aagccctctt tccataaagt atacgctata tttctttgaa
     1201 agtatgtttt tacacaattt tttcataaag ttcataaaaa attcgaattg ctgctactaa
     1261 tttttcgttt tcgttgcttg ccctgtctca taattgatgg tgtagccatc ttgaacattg
     1321 tagataaaga cattgaattc aatttttttg tcttcaatgc tttctgcttc caactgcaca
     1381 cctcttgcta ccagttcatt tccctcgaaa tgaggagtga cacgatagcg tacatgatga
     1441 tttgtttcct tgatatatgc cgcaatatca ttttcgtggg caagcatgta cggagcattc
     1501 aacgaacgtg tccctgtcat taagttttta atgttattat tttgaccagt caactggaaa
     1561 ccaatcaaat ggctgcggtt gtataaccat tgtccgtcac ttaatttctt ttgtttccaa
     1621 cctgtcggat caatatacaa aggttctctc ttttcagtag gaaacaagtc ttttcccaac
     1681 atcgcattgg cttgtcctac tctgttcaat ttgtctatat ttgaatagct ttcccatgtc
     1741 cctttggaaa gactaagatc ctcttttgta aactccggct gattcccatt gatttctatt
     1801 tcttgatgtt ttccatcata ttctggcaca tccgctactt gagaagcttg cggtgtggaa
     1861 tcaaaaatgg ttgtaatttg ttggaagata tctgaatctt tttgtgtggt ggaaataccg
     1921 agtgcccctg caatcacagc agcgacgatc cccacgatcg ccttggttgt ttttttgctc
     1981 atttctttac tcctttacta acagttccct aaatcttaac atatgttccc ctataaataa
     2041 atactggaaa gaaatagaaa cagtaaaaaa ataaaaccag tttttagacg gcttaagaat
     2101 acaaaaaaag agacggattc atcgtctcta ctcaatcaca aaacactatt tacaaggtat
     2161 ttattatcat tcaaaaaatt tttcttaata ctcaaaacta aacggcttct gtacgacctc
     2221 ttggtaaaca gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa gtcaaaaaca
     2281 taagaagctt ttttgcttga ctattcttat tacttgatgc agggcagctt tttcacagcc
     2341 tcttttaagc gggtgacggg aatcgaaccc gcgacatcag cttgggaagc tgggatttta
     2401 ccactaaact acacccgcga tacaaaaaca gtatacgcct ctttcactcc ttcctcaagt
     2461 ttttttattc tttacgcttc cgatatgttt tgtagtaaaa taaaactaat caatcaatta
     2521 ttggagaaga tgctatgacg ggaaaagaat tagatctgtt gctggacact tgtttactcg
     2581 ctgggaaaat catgatggaa agcaatgcgg agatgtatcg ggtagaagat acgatgagtc
     2641 gaattgcttt agcttctgga aatttccgtt tggtcagcta tgtcactcaa accggtctct
     2701 ttattggtct tgatcgtacc tcgaccatcc gaatggaaca aattaccaac cgttcgatta
     2761 atttagaaaa agtagtaaaa gtaaatgatc tttcacgtaa atatgttgct tcagagctaa
     2821 cattagaaga actttactgc gaactgcaag caatcgaccg agaccgttct ttctttcctg
     2881 tctggttgca aatcgttagc gcggcagtga tcagtggaac aattatggtg ttatttggcg
     2941 gaaagatgac cgatttacct gtgactttag tcattggagg attgggatac gcagcttatc
     3001 tatatagttt gaaatttttt agaatcaaat ttttatcgga atttttatct tctttattga
     3061 ttggcatagc cgctatctac agcgtacgtt ttggcttagg aagcaatcag gatctaatca
     3121 ttatcggatg tgtgatgcct ttagttcctg gagttcaaat tacgaacgct atccgtgatt
     3181 tgctagctgg acattatgtc tcaggagttt ctcgcggaac agaagcgatg atgactgcta
     3241 cgatgatcgg ttttgcgatt gcttttattt tccagctttt ttattaggaa aggaacacaa
     3301 gtatggggac gatatttatt cagttttctt ttagtttttt agcgactgcg gcatttgccg
     3361 ttattacaaa tgtacctagg cgttcattga tttgttgtgg tctttctggt gcttttggct
     3421 ggatgatcta ctggatctgt gttcaaatag gcggatctgc tgcttttggt tcattgcttg
     3481 gttctttagg agtagcattc atcagcgatc ttttctctaa acgtttgaaa atgcccgtaa
     3541 cgattttcaa tattcccggg atggttcctc tagtccctgg tggtttagct tatcaagcgg
     3601 ttcgaaatct cgttactgaa tcctatcgag aagcggctca ttatgcggtc caagcaatta
     3661 tgattgctgg agcaatcgct ttaggtcttg tattatcaga agtctttaac cataacatcc
     3721 gtaatttcaa agtaaagcgt gacgcaatca tgatgaaacg aaaaaaacgg ctttttaaat
     3781 aaacgaagtg taacaaagaa aaggttgtga aagagacgtt cagccttgag cgatgacaaa
     3841 atatctcaca aacattgcga caagtaaccg caggagcaat aagaagtaat tatgagtatt
     3901 aaaaaaagtt ttgtaaaaat agcttctcac atttttacat aaaattgact taatactgag
     3961 aaactgactt ttgaacaccc tctatctgta aaaaaggggc tgcgacatga tttttgtcac
     4021 ggcccctctt ctttattcaa attttttcca aaacgttcgt tgataataaa cgatccatag
     4081 agcggaaagt attagaacta aaccgatcac aggaatattc actaaccaaa atggataaaa
     4141 caaagccaac attccgtata agacgataag aataatactg atgatcaatg aacccatcat
     4201 agttgcgact aaacccaaac ttcctgaacc tctgttaaat aattggttaa tattggtcca
     4261 gttcagcagc agcaaccgat aatctctagc gaaataacgt agtgacaata agtagcagcc
     4321 gaataaagaa ccaaatagca agctagggat aaaaaacaaa ggcagctgaa aagacagtcc
     4381 gcttaataat gcgatcccac cagttaaaaa actctgcaat atccatccga atctgaactt
     4441 ttcttttaaa tattggttca tagagattgg caaagaacgg ataaacagaa aattttcttg
     4501 atccaaagaa ataagattgc tgataaaaga agtctgatta actgttaaga aagccaatgc
     4561 gatccctacc aggaaaacaa caccgattaa cttattatct aaatcagtta attttatttc
     4621 gcctcctagg gcaaaagcaa gaataaaaat gattggagtt aacaaggaag aagaaagaac
     4681 ttgcataatc aaattaggtt cttttactaa ttgagcattg taactaaata aaagctgatg
     4741 cacattttgg ttctttttat gggttctttt tattttgaac tcactgtttg aatcagtcag
     4801 ttgctctgta agctttggta ctatcagctg ttttacagca aggaaaaatc ccagagccgt
     4861 gatagctagt attccccaac ccaataaacc ttgttgagaa agtggctgac tggcagcatc
     4921 atagaatggc aataatatcg aaatcggatg ccgatctgtt aactcatttt caatagtccc
     4981 cgtttgatga ttcatccaca tgattccagc aaatgcgatc agcatcgagc tgccaagcaa
     5041 gatactagaa actgtttttc tatgtttttt aaacactgct gttcgagcca aaccaaaaac
     5101 aatcaaacta gccagacaaa aaagaataat caaaaataga gaaaacaaaa ggattgagat
     5161 aatgatagta agcaaaatcc ctagatcagc tcgccatccg gttaaaataa acatgactag
     5221 aagcggaaaa acaaaaggaa tgatggtcaa agcaactacc aaaatctttg ctgaaaaaac
     5281 ctgttcttgt ccaaaaggca gaggcaaaaa tacttgcagg tcatttcctt caaaaaagat
     5341 attataaatg actgatatac tttgagttaa acctaacata ccaaaaagtg ccacgtaata
     5401 agtaaagaaa cctggcaatt taacaaagtt catagcaaac atagcaaatc cgtaaatcag
     5461 taaaaaaatc agccctgaaa aaagattttg agacaaaaga tagatagtta atcgctttcc
     5521 cttttttcct ttctctcttg ctttattcgt gagctgtgga ttaacatata aaagattaac
     5581 tcgaattaat tctaataatt gtcgcttata catacggtcc atctcctgtc gcttgccttc
     5641 cagccatttt caaataaatc ttttctagcg tttcatcagg tgagccattc aataattcac
     5701 taactgcccc ctgataaatc aattcacctt tcttcaaaat agccagttta tcgcataact
     5761 gttgtgcggt atccaataca tgtgtagaaa aaatgactgt ttttcctttt gcggcatgag
     5821 ccttcatcat ttctttcaaa tcaaatgctg cttgaggatc aagtccttgc aaaggttcat
     5881 ccaataccca aatatctgga tctggcagca acgtgccgat caaaatcgtt ttttgacgca
     5941 ttccatgtga aaaacttgcg attgtttcat tttgatgact atacatatca aaaagcgctg
     6001 tcaattctgc taaacgcttt tctttttttt gtgtgtctaa ttcataggca gctgaaatca
     6061 agtcccaata ttctccagcc gtcaattgta aaaaaatatc tggggaatca ggcacataac
     6121 cgattttttg tttgatactc aatcgatttt ccgtcaattt cattccatct actaaaatcg
     6181 ttcccgatga tggttcaata atactaacta aacttttaat cgtagttgat tttccggcac
     6241 cattatgtcc taaaaatcca aaaatttccc cttgtcttat cgagagagac aagtctttta
     6301 atgcttgttt tttcccgtaa cttttactta cattgatgaa ttcaatcatt tctgtactcc
     6361 ttttgtttgt tattactttt tattgtaccc ttcgttattg gtggagtaaa tgtcaaaaag
     6421 agtgcataac aaaaaaggat agaaacgact aggctgctac ttgggttgtt ctcttctaca
     6481 ggaaaacact cttctaacag gtaagatatg agtctttgtt ttctttaaaa tgattcattt
     6541 tctatctttt cttgcataaa attctttagt ttatcaatac ttctctttcc catctctagt
     6601 tgttctccat ttgcaaatgt tactatttcg tttactctgc taatagatgt aatgttttca
     6661 gtattaatga tataagcttt taaatcttta cataaatact catattcgag tgatttttta
     6721 tattttttaa ttgtgccgtt tacgagaatc tcttcatctt tcaatactag gcctatttta
     6781 tttttataac ctggcatgct cattagataa aggatttcat cgtaatggat atagtgatcg
     6841 ctattaccat aacggacagt tgtccacctt tttttcgtag catgttgaac aggatagttt
     6901 tctttttctt tcaataagta actttggatg ctttttttat ttttttcata agtgaattct
     6961 tttatcaaat aaccactagg cgtaatcttt cgattaataa cagttattgc tttattcgga
     7021 tgagacgtta gataaacaat tttacacttt tcattctttt ctctaagcat ttcgcctagt
     7081 tcgataccgc tataataggc gttcaaatcg atgtctagga aaaaaatatc tgaggaatag
     7141 atttcattat ttttaatgaa ttcaacaaat tgtataacgt tcaatccctg aacgatctca
     7201 aatttccaag gacttaataa aattctggat tcttccaaaa tttctactag cattttttca
     7261 aaaaccaaac tatcatccaa aataaatata cgcgttacca taaaaagtac ctccactcga
     7321 tattttacta tcaaaatcat ttcatacaac ttttcaacat acatgttagg aataaattgt
     7381 aattctcttc acaatctttt ccaacacctt tgaaaccgat atcaaatact ttcaggatgc
     7441 tgcttctaaa ataaatatca aacaaatgaa tacatcatgc aaaaaaagcc agacaaccga
     7501 cgtccttgcg tgtcagctgt ctgagctatg tttatttatt ttgttctttt aaaaagaacg
     7561 atacacatac tgatttttcg gagcagagac tttctcagcg tttgtaacaa ctacagatgg
     7621 gatttcacgc ttgaatggtt gataatagtc tgattggatg gttccttcta ttttatacca
     7681 ttcatcattt ttaaccgtta ctccttccgg catatgcgtc aacaaaccaa agactcctga
     7741 atctgccaca caatggataa tgccgaatcg gaacaagaag atatctgtat tgctatcgtt
     7801 cggtgtttgg taaacaaatc cttcatagct gatttttttg ccaatgaatt cgctagggta
     7861 attataaatt agctccataa cttctaaata gttttcatct gtgatcttga tagtttgctt
     7921 atccccatac ttttctttca actcattcat ttggtctaag tagtctgatt tgttgaaata
     7981 aatactggta tccggcttta agtattgtgt ctgcatctcc ggatccccta ctgattcttt
     8041 acttactggg aaattaaatc ctttggcttc tacgatcgta gtgtcgagac taactgttgg
     8101 aaacaacgtc cccacgatta ctggcaaagc tagcagtaca taagcgattc cacgttgata
     8161 aggttttgac aacccatggt catgatcgtg ctcgtgtgcc ttttcttctg cttttccgtt
     8221 tttgacccac aaatacagct gaactaaagc caaaagagca gataaaatca ttgaaagaat
     8281 agctaagtag cgataatgga cattgatata ttgatcgagt ttgccagaaa cttgcaagta
     8341 catcatcaat tcgaaatacc cagataaaat caaaaaccgc agcatatttt ccctcctaaa
     8401 taaataatgt gaatccaata acgctaaggg caatcagtcc gatcagcgat aaaataaagc
     8461 gtcctgtaaa atatcgtttc atcatcagta gatttttgat atcgaccatt gggccaaaga
     8521 ccaaaaaggc agctacaggt gctgtgccga acagacttaa caaagaagat cctataaacg
     8581 catctgcttc tgagcaaaga gacatcgtaa aggccaataa cagcatgatg ataatggcta
     8641 aaatctttgt tgagccgagc gttagcataa ttctagttgg taaataagtc tgcatcgcag
     8701 ctgcgattaa tccgccaatg atcaaataac gacctgtatc aaaaaattcg tcgattgaat
     8761 gtgtcaacac atgcccagtt tgatggatca gctgattttt tggttcattc ttctcacttt
     8821 gttcttcttc atggacatga tgatggtgat tttctgctac tttagacgtg attttttctt
     8881 tcaatacagc gtctttttga aaataggcaa gccagacacc aatcacaagg gcaacaaaaa
     8941 agctgcctat cacccgccag cttgccatct gccaagaatt gccaaatgcg ataaaagtgg
     9001 aaaaaatcac gattggattg atgatcggag cagtcagcat aaaagcaaac gctgtgtgaa
     9061 ctggtacgcc ttttttaaca aattgatgaa cgatcggtac gatgccgcat tcacatgaag
     9121 gaaagaaaaa tcctaagatc gaaccagtaa aaatagagaa gatacgattt tcaggaagcc
     9181 atctcttcac acgttctggg gtcaaaaata cttgtaaggc gcctgagata agacagccta
     9241 ataatacgaa aggtaatgct tcaataacga tcgataagaa aatcgtactc atctgcaaaa
     9301 ccgaatttgg aataaaagaa aacatgagtt tcctcctttt tatttctagt tcattcgctt
     9361 tgcaaatata cgcttttatt caaaaaaaac aagttgctct gtaaattttt acacaactct
     9421 tattttttac caaagcactt caattgactt tttctgttat tcccattaaa ttaaggtctc
     9481 atacatatca cttctatcat aaaagggaga ataaaaatga aacaaaccac taacttgact
     9541 gaagttcaag acatactgca acagtcatcc gttgccgtca tttatctttc gatgcctaat
     9601 tgcagtgttt gtcacgctgt tcgtccacga ctagaggaac ttctcacaca ttatgatatt
     9661 ccggcacttc atttggatgc atttgaaaca ccagaagtag ctagtaaatt tgaagtgctg
     9721 actgcgccag ttgtcctgat ttttcatcaa ggaaaagaag tctttcggca agcacggttc
     9781 atcgacttta aaaaaatccg ttatctacta gatgaactga cagcagaaga tgattccctt
     9841 tcttatgaag aaatcttccg aacaaaataa tcgttgtcca aacagaaaaa agcgattgaa
     9901 caagtgaaag ttttgccttc atttgttcaa tcgcttttta tttttattct gctgtatttt
     9961 cggaatgcgc atttgcgtca gaatgcaaga aatagatcaa ggtttgcaat tcattcgtca
    10021 aatcaacatt ttggatcaat acatctgacg gagcattcaa tcgagcagcg gtaaagttca
    10081 agattccttt gattcctgct tccgttaact gattgactac ttcttgtgct ctttcagcgg
    10141 gtatcgttaa aattgctact tcgatttgtt ggatcttgat ttgttctgac atatcttcca
    10201 ttggataaac ggggataccg tctacgatgc gcccaacgat ttcctcattc acatcaaaag
    10261 cacaactgat acgtatgcta ttactttgat ggaatttgta ttttagtaaa gcacttccca
    10321 agttccctac accgatcagc gcaacatttg tcaattgatc gtcgtttaat gtctttgcaa
    10381 aaaagtgcat aagatcttct acatcataac catacccacg ttttcctaat tcgccaaaat
    10441 aagaaaaatc tcgacggatc gttgcactat ctacttgaac ggcttcactt aattcagttg
    10501 aagacacttt tttctttcca gcattattta aaatccttaa atagcgataa tacaaaggca
    10561 gtcttttagc tgtcgccttt ggaataatat gatctttcac gaaatgacct ccacgtattg
    10621 tctaattaca tctttgattt ctccatcata gcagtattta taggaaaaaa tcaattccat
    10681 tgctcagata ttgtgaaatt ttattcttta ttcatttatt tatcatttct gcaaatctcg
    10741 cagtataatg gaatttatgg tagactacta attgaaaaat agttttttat ttgaggagaa
    10801 ttcacaaagt agaaaagagg attccatgat attattacaa gccaatcaga ttgctcgcca
    10861 ttttggtgca gaagtcttat ttgaaaacat acatttagaa atcagtacag gcgctcgtgt
    10921 cgctttagtt ggcagaaatg gcgctggaaa atcgacttta ttaaagatca ttgctggaat
    10981 tgaagcacct gatgaaggaa agatcgctaa aaataaaaca gcgactctcg gatacctcgc
    11041 tcaggacaca ggacttgatt ccaatgaaac agtttggaac gagatgctga aagcttttga
    11101 acaagttaga caaatggaag agcggatgcg cgagctagag attttcatta gcgaaggtac
    11161 gccagacaca cctgcttata gttcttattt aaaagaatac gaccggctgc aacacgattt
    11221 ttcagatcga aacggctatg gttatgaaaa tgaaattcgt tccgtattgc atgggtttaa
    11281 attcgattct tcattttatg atcagaaaat cgatacttta tcaggcggac agcgcacacg
    11341 tttagcttta gctcgcatgc ttttgcaaca ccctgacatt ttgattctag acgaaccaac
    11401 gaaccattta gatatcgaga cactttcctg gcttgagaat tatctgcaaa gctatactgg
    11461 tgcattattg attgtttccc atgaccgtta ttttttagat aaagtagtca atgaagtcta
    11521 tgaattaagc agaagaaaaa tgcgccatta caaaggtaac tattcgaaat atctagattt
    11581 gaaagcagaa cagttagcaa gtgaatggaa ggcttatgaa aagcagcaaa cagaaatcca
    11641 taaattagaa gacttcgttg ctcggaacct tgttcgcgcc tctaccacaa aacgagcgca
    11701 aagccgcaga aagacattag aaaaaatgga ccggctggat cgtcctcaag gggatgaaaa
    11761 acaagcaaac tttctattca gtatagaaaa gccttctgga aatgtagtct tgcaaatcga
    11821 agatgccgct atcggatatg ataatgtcac cttatctgaa ccaatcaatc tagatatccg
    11881 tcgtcaagaa gcgattgctc tagtaggtcc gaatggtatc gggaaatcga cgttgctgaa
    11941 atcaatcatt caccagatcc cttttatccg tggaaaagaa acgctaggga caaatgtcac
    12001 catcggctat tacgatcaag gtcaagctga tctgcataca aacaagacga tcctagcaga
    12061 attgtgggat gagcatccta ccactccaga aaaagagatc cgaagtgctt taggtgcttt
    12121 cttattttct ggcgaagatg tagaaaaacc aatccctctg ctaagtggcg gtgaaaaagc
    12181 acgggttgct ttagccaagt tagcgatgaa caaagaaaat tttttgattt tggatgaacc
    12241 aacaaaccat ttagatattg acaacaaaga agtattagaa aatgcattga tcgattatga
    12301 cggcacactg ctttttgttt cccatgaccg atatttcatc aatcggatcg cgacaaaagt
    12361 cgtcgaactc tctgaaaatg gcagcaaagt ctatctagga gactatgatt attatttaga
    12421 gaaaaagcag gaagaagaag aaatcgccga attgttggca caagatgagc cagaatctgc
    12481 tcctgctccc acgaacaaat ctgattatta ccaaagcaaa gaacagcaaa agcttatccg
    12541 tagccttcaa cgaaaaatta ctcaggttga agaagaaatg gcgcggatcg atgaatggat
    12601 cgatcagttg gaacaagaaa tggtcgcgcc agaaaacctg gaagatcacg taaaactcaa
    12661 tgagctgaac caagatttag aagctgctag acaagatcaa gaaacaaaat tgacggaatg
    12721 ggaagaacta agcttggaat tagaagaata tgaaaattaa cgaaaaaacc aaaacagaaa
    12781 ccattcatgc tgttttggtt tttctttttt tattttatca aacaaaaatt ccatatttac
    12841 ctttttcacc aataagaaga ccaattacta gtcattctca ctaaagcttc taaataatag
    12901 taatcacccc aaatacaagg tgtatttacc ccattatttt tgggcttatt ataaacagac
    12961 tgtgttaaaa agccatcttc ttcttccgtt gtagcatatc tttctttcaa ttgtcgcata
    13021 attttccaaa cagcagactg gtataagttc ttttctgttt tgtctaatag acctgattct
    13081 attaattcta ataatccaca aaccatgatc accatactag atgtatcttt ttcttctctg
    13141 ctagaatcat caaagatcaa atcccaataa caaatggtat cttctggcaa ccgattcata
    13201 aaataattgg ctaatatttt gcttaaatca aaatattttt gttctttcaa ataacgataa
    13261 cttaatgcaa aaccgtaaat cccccatgct tggcctctag cccaacagga attatcacta
    13321 taaccttgat gcgtacttcc atatcttggt tttccatctt tgacatccat ataaaatgta
    13381 tgataagttg aaccatcttc tcttactaaa taatctgctg cattttgaat atgagcctga
    13441 gcagcttttt tatatttttg atttccagtt tcttcactcg cccaatacaa taatggaaga
    13501 ttcatgcaac aatccatgat cattctacct tgttgtgaag gatcactagg atctccccaa
    13561 gcttgtataa ctcctgcctt ctcccaaaaa cgctgaagta atgtttctgc agcatcaatt
    13621 gaagcttcct tcatttttcg atttcccgtt attttataat ctgctacggt tgataaagaa
    13681 tacaaaaaac caagatcgtg atgattagtg ataatttgac agtcaatcct gtggataaaa
    13741 tctatatctg ctttttcagc tgtttgttga aataaaggat cacccgtctt ctcaaataac
    13801 aaccaattaa ttcctgtcca aaatccttct gtccaaccaa tattgtctct taatggataa
    13861 cgtcctttta taggacactc gcttgggaat tgatactgac tgttactgat acgtttgatt
    13921 aacctctccg tgtgtctgcg acaacgctcg atctcattat tccattcttt ttccattaat
    13981 tctttataac attcatttat ccatttttct ggatgtatta tctcttcaaa catcacttta
    14041 tcaagcattt ccccaccagc ctttagcata attttagtct ttttttattg tttttatttt
    14101 ggatagttag cccggttgtc ccatcaaaat caattttggg acgattgttc catacttcta
    14161 aactataatc ccgatcttga tgagcaacca ctcctgttcc gagccaatgg gttccttttc
    14221 caaatgtacc ctctaatcca ggaataaaag tgttttccca tgtcatcaag tttgtattgg
    14281 gcaaacccat aatagctgtg gattttcgtt taatagtatc gtcacttaaa cagataatac
    14341 ctgaaaagcc agcttcactc accctatagt tttccttgtc ttcttctatg attattcctt
    14401 ctttctcagg agaacattca atagcaaacc ctgtctcata tagaacatat tctgtatcta
    14461 attctacttt gtgtacgcga atctgccagc cttcaaaagg aataagccaa gttctgattt
    14521 ttgcctgtaa agtaggttcc catgtagagg caagtacctc tttcttacat acaatgtctc
    14581 gtacattatt tcgaacgatg aactgtccat aggttttgat gctctcattc ttaaaactgg
    14641 tcgtgatata tgtgtccgct cgctgaatcc ctagcattga atccattgcc atttctgctt
    14701 tcaattgatt cgctcgcggt atcgaaaaac caaattgact agaataagca aattttaaat
    14761 atttttcatt ggtcaatgta tggaaatttg gccctaattg tcccgcattc aaaaaaaatg
    14821 tatgtccatt atcatgttgt atgagcaaat tggcttctga aagaagttta gttccttcac
    14881 gaataggcat aggcatttct ttagccgtcc aatactcatg gtcttcaggt agcgccaaca
    14941 ataaaaaaat cttattcagc cataatggag ataacgtact attatagggt tcagtcaatg
    15001 tcaattgagg gtaggtataa ccaatattca ggataccttt ttcatcaaat atttcttttt
    15061 gcatccacca tcttatattc cgattgacta accccttgat ttcacctaat ttaaaaggca
    15121 aaagatcggc ccacaacata gctgaccaaa agcttgaaac tgcaaaacga taaatcatgc
    15181 ttcgaccaaa aggcacattt gcaccatctt ccgcaaaaaa atgaatatag tctctagaaa
    15241 aatcaatagc tcggttctta tatatctggc taatgggctt ttctggttct agtacgctat
    15301 aaatcaaacc ataataatga aatgcaaaag gattataata atcttggcga cctcttgaac
    15361 tgtctcgata ccagccattt tgaagataac aattttctat attatcaaat gcttctttca
    15421 gtgctgattc tttaactgga taaccaaatt tttggataat tgttccgaca attatcttaa
    15481 aaaattgcca attaccatct gcacattcat attcaaaaat ttgcgagaac caatgaataa
    15541 tctggctttt ttctcctgta tctaattttt tccataataa ttgttcatga tgatataacg
    15601 ctaataacat tgcgggcatt tctaccatac gctggtctct ttcatgtatt tctccccaat
    15661 aatcgggatg ggaaggatct gttccagctt ttattcctga tagaattaat ttcaaccatc
    15721 gttcatctaa ttggtcaatt gcgggccccg ctccccataa aacccgagag aatccttcca
    15781 ttcctgcaat tttatttcca tatcctaccc cttgatgtat ataatcaatt tgtgcgccac
    15841 ttgctcgata aaacggctgg agtggttgat ttatcttttt aaataagtca aagtaatcct
    15901 gtctggtcct taatagtggt actggatgtt tcattgtatt ttctcctctt tttactacca
    15961 aatagctaat tccgactgtt ctaatcgaag taagccttca ataaaaaaat aatcagaata
    16021 gatcagtggc acattataag tcagttctct atggtatgct tctgctccgc ctccaatgat
    16081 cccatcttca tccatattcc aatttgcata gtgcttttct gtcttttgga gtatattgat
    16141 agcagcttca tgatacattt ttgatcgtac agaggaaaca tatcctgcta attccagaag
    16201 accgcatgca gcgcatactc cagcactggt atcaatattc gaatctttta cagcaaaatc
    16261 aatgagagga atataaccag ttttttcgat tttctgaata aaatttactg ctatcttttc
    16321 tgctatagct agatagtacg gattttttgt gtaacgataa cttaacgcga atccatataa
    16381 agcccatgca tttcctctgc tccaacaaga attctcattg tatccttgcc cgccaagtaa
    16441 ccgaatatat ttccctgatt ctgcatcaaa ttctgcgata tgcccggttg atccatcttt
    16501 cctcactaga tacttagctg ttgtatctgc atgctgccga gcaattttag agaacctcgg
    16561 atcaccagtt tccctcgaag cccaatacag taatggtaaa ttcatcatac tatcaataat
    16621 catccaacca ggtttatggt cattccatgc tactaaatag ttccctgaaa tattatagcg
    16681 tccagcaagt accgtagctg catgtaaccc tagttgtcga ctatgtttgt tttgagactg
    16741 ttgataatgt gcaatagccg atggtaagaa taaaaaacct acatcatgat gaacgtctga
    16801 aaaatggtac agggcagatt ctaataattg ttcttgttgt attgctgcct ctttgaactt
    16861 ttctcgtttt gtcttgtgaa agagctgcca taaaatacca ctccaaaaac cattggtcca
    16921 ccagtcgatt cctctgtcct ttattacgtc tgtatatttt ccgttgtaag aaatatatgg
    16981 aattttattc cccattctgt ctaattccgc atctatcttt tcagaagcta cattcaaaat
    17041 ttttcttgac cattccttcg tctgttcatt catggatatc ccgccattct tttaattttt
    17101 atgaatgtgg agttgctcct agaacgaact agatggttaa cattcgttat tttttttact
    17161 tatttgcagc tccaatgtca ttctccttaa atttagactg cgcccacaca taaatcaaga
    17221 ccattgggat cactgaagtt gctaaaatta gcataattgc gccataatta attggctcac
    17281 ctgtactaga aaccgtcata tttttgataa ttaagggaac cgtatattta tctttatctg
    17341 aaagcatgac taatggaaat agatattgat tccaacttcc cataaagata agtaaaccac
    17401 tagtaataat cgtaggtgat aaaatcggta cagcgatttt cgtaaaaatc acccactcat
    17461 tggcaccatc gattctggca gcctccatca gtgctgtagg aaattgactg ctatattgtc
    17521 gcatcataaa cactgaagtt gtactggcaa tcgaagggat gatcacacct gcataagtat
    17581 tcgataatcc taaacgactc ataatgtaaa ataaaggaat caatgtggta aaaaaaggca
    17641 ccatcctaga tagcataact gtattgaaca acagtttatt ccccataaaa cgatactttg
    17701 ctaatgcata acccgccatt gtagaactgg ctgtagctga aactgtactg attacagcaa
    17761 taacaatcga attccaaata atcaagggat aattatatct cgccatgatg gcttgccagt
    17821 tttttaatag catatcccca aacttgaaat tgattccgcc acttaaaatt tcttgattac
    17881 tttgcgttga agctacaatc atatataaat atggcatcaa aaatattaag gcaccaaaca
    17941 caataatgac gtacgtaaaa attctgcttt gtatcttttg tttagtcatt tttatcacct
    18001 ccgaatttaa attgaatagc cgataatata acagatacaa acaccatcgt ccaggcaata
    18061 gcagaggctt ttccaaaatc aaccatttca aaacttgttt tatataaatt gactcctacg
    18121 accaaagctg aattttctgg tccattggaa gtcccaaaga atatatttgg gatattgaaa
    18181 ttacccaagc cattgatagt ggctaaaact gtggccatta aaatgattgg ctttagcatg
    18241 gggatcgtta ataaaaagaa ttgccgaaat ttcgatgctc catctatttc agcactttcg
    18301 tatatctctt tagaaatccc ttgaagacca gctaaaaata aaatagtgtt atatcctaca
    18361 ctaagccaaa tcatcgtgat gatcattgat attcgtatcc aaaaaggatt tgttaaccac
    18421 tgtacaccgt taattcctag cgcattcaaa aatccattga ataacccagt cggattgaaa
    18481 aaagtcatga atacggaagc tacagccacc gttgatgtaa tggttggaat ataatagaca
    18541 gttctaaaaa atccttttcc ttttaactgt gctgaattca ataaaacagc aaagtaaata
    18601 gctaatagca tcgttattgg aattgatcct gccatgataa tcaaaacatt cgtgtaagat
    18661 ttaataaaca atggatctcg gaagatagct agataattgt caaatccaac aaattcaaat
    18721 gagtagccaa ttttcataaa actaaaagca aacgagataa tgatgggaat caaaatcgtc
    18781 acaataaaga aaataacaaa aggtgtgacc aatccataag cacttttttt atgagacgta
    18841 tctgttgctt ttttcttgct gttctttatt gtcttgcttg gtaacgaaga ttgcatcatc
    18901 gttaagtcct cctaatattt tttaatcacc agttaatttt acgccgtact tatgagcaaa
    18961 attttctgct tcttttagat atgaattttc aaaatcacct gtttgccaat agttatatgc
    19021 agcggtagtc aaataagacg tagcatctga ataagtggct ggtaaaacag tatttgatgt
    19081 attttcattg attttattca atagttcata gtatttttgt cctccttagt agtcaaactt
    19141 tttctcagct gcttctgttt gataggccaa tgagttagat gtagccacgc ccagctccag
    19201 tgcgttggct aagcattctt catctgtcag cataaatgta agaaattg
//
LOCUS       DAZULG010000055        18640 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000055 DAZULG010000000
VERSION     DAZULG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.056"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1268
                     /locus_tag="ACG9CY_001655"
     CDS             <1..1268
                     /locus_tag="ACG9CY_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HFS4938166.1"
                     /translation="IGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKEN
                     QFVHKDDVLVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSF
                     GYSNQLKSMLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQV
                     QVAWSNNQSLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEV
                     EQYQSEKAKLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKS
                     LDDQLSKGILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANE
                     STYIKEGMKVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPF
                     RYGLTGELSLIVGEKTYWQQIVDVFLNKK"
     gene            1301..2335
                     /gene="sppA"
                     /locus_tag="ACG9CY_001656"
     CDS             1301..2335
                     /gene="sppA"
                     /locus_tag="ACG9CY_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4938167.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            2337..2858
                     /locus_tag="ACG9CY_001657"
     CDS             2337..2858
                     /locus_tag="ACG9CY_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4938168.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            2855..3241
                     /locus_tag="ACG9CY_001658"
     CDS             2855..3241
                     /locus_tag="ACG9CY_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4938169.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(3737..3809)
                     /locus_tag="ACG9CY_001659"
     tRNA            complement(3737..3809)
                     /locus_tag="ACG9CY_001659"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3774..3776),aa:Lys,seq:ctt)
     gene            4190..4759
                     /locus_tag="ACG9CY_001660"
     CDS             4190..4759
                     /locus_tag="ACG9CY_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS4938170.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            4746..5606
                     /locus_tag="ACG9CY_001661"
     CDS             4746..5606
                     /locus_tag="ACG9CY_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4938171.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            5796..6500
                     /gene="yycF"
                     /locus_tag="ACG9CY_001662"
     CDS             5796..6500
                     /gene="yycF"
                     /locus_tag="ACG9CY_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS4938172.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            6505..8340
                     /gene="walK"
                     /locus_tag="ACG9CY_001663"
     CDS             6505..8340
                     /gene="walK"
                     /locus_tag="ACG9CY_001663"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS4938173.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            8337..9653
                     /gene="yycH"
                     /locus_tag="ACG9CY_001664"
     CDS             8337..9653
                     /gene="yycH"
                     /locus_tag="ACG9CY_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS4938174.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            9654..10523
                     /locus_tag="ACG9CY_001665"
     CDS             9654..10523
                     /locus_tag="ACG9CY_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS4938175.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            10578..11387
                     /locus_tag="ACG9CY_001666"
     CDS             10578..11387
                     /locus_tag="ACG9CY_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4938176.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(11490..12119)
                     /locus_tag="ACG9CY_001667"
     CDS             complement(11490..12119)
                     /locus_tag="ACG9CY_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4938177.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            12289..13581
                     /locus_tag="ACG9CY_001668"
     CDS             12289..13581
                     /locus_tag="ACG9CY_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4938178.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(13657..14319)
                     /locus_tag="ACG9CY_001669"
     CDS             complement(13657..14319)
                     /locus_tag="ACG9CY_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4938179.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            14544..14828
                     /gene="groES"
                     /locus_tag="ACG9CY_001670"
     CDS             14544..14828
                     /gene="groES"
                     /locus_tag="ACG9CY_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS4938180.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            14879..16504
                     /gene="groL"
                     /locus_tag="ACG9CY_001671"
     CDS             14879..16504
                     /gene="groL"
                     /locus_tag="ACG9CY_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS4938181.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(16593..17732)
                     /locus_tag="ACG9CY_001672"
     CDS             complement(16593..17732)
                     /locus_tag="ACG9CY_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4938182.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKMIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYGEGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLSNRAKELIESVIA
                     DNILAGRPTNPDQYIFISTNGTPLTLHSFNAVLHKVEEELELEKNLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKKAKQQVIDKLNRL"
     gene            complement(17843..18427)
                     /locus_tag="ACG9CY_001673"
     CDS             complement(17843..18427)
                     /locus_tag="ACG9CY_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS4938183.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(18554..>18640)
                     /locus_tag="ACG9CY_001674"
     CDS             complement(18554..>18640)
                     /locus_tag="ACG9CY_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFS4938184.1"
                     /translation="IQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         6217 a         3058 c         3830 g         5535 t
ORIGIN      
        1 ttattggatt gtttttaacc tttgctaaaa aagaggttgt tattcggaca acggctcaat
       61 tgaaccccga aaaaatagag aagttacaag tgccattaga agctaaaata atggaaaact
      121 tcctaaaaga aaatcaattt gttcataagg acgatgtgtt ggttagattg gattgttctt
      181 taatagagaa tgaaaaggct cagatagaac aagaaaacca acgtattact caacaaataa
      241 agatggctca gctatttatt gaaagtataa gtaaaggaaa aaatttgttt tcaacggatg
      301 acagttttgg ctacagtaat caattaaaga gcatgttgtc agaaaaagaa tcactccgct
      361 acgctttgaa gcaaagtgaa ttaaatgatc aaaagcaatt agaagtatac gaaaagacaa
      421 aaagacaact agaaaaacaa attgagagtt cagatagtaa attacaagaa tggcaacaag
      481 tacaggtagc ttggagtaat aatcaatcat taaaagattt ttcaaaagaa atgatggcaa
      541 actatgagaa ttggcaagaa caactaaata atgtttctga tgatcaaaaa aatcaagtga
      601 aactgacaat ttcagcaagc ataaatgaac aaattgagca actaaaaaaa gaagtagaac
      661 agtatcagtc agaaaaagct aaattagtta aaccaactac ttctgagaat gacagaatta
      721 gtcaaacgga aaaaggaaag caagagctag aacaagcaat aacaactgta aaacaaacag
      781 ttacagagct aaatgaaaaa caagagaaga accagtcagt tatcaaaagt ttggacgatc
      841 aactttcaaa aggaatacta aaagctcctg tatctggaac ggttcattta aatgaagaaa
      901 caaagggaca agcagaacta gccaaaggaa cagtgcttgc agaaatttat ccgatccacg
      961 aaaaatcaaa aatgaaattt acagcacttt tacctgcgaa tgaaagtacc tatattaaag
     1021 aagggatgaa ggttcatttt aaattggatc aaaaaggtgg ggctccgatt acgatcgacg
     1081 gaactttaga tgaaatttca gaaaattcta ctgccactga gaaaggtgtt ttttatgcag
     1141 tgaaaggaac gttaaaaaca actcaatcgt ttccgtttcg ttatggatta actggagagt
     1201 tgtctttaat tgtaggagaa aaaacatatt ggcaacaaat tgtcgatgtg tttctaaata
     1261 aaaaataatt atttttacta agaatatagg aggagtttat atgaataaaa gaagatggat
     1321 tgctgtggga gtagcgtgcg gattattgtt actatccatt atcgtcgcgc taattccagg
     1381 aaaagataaa gaggaagcat caaatacaac cctaacagga ataaataaga ttttttacgg
     1441 atctaacgaa atcacagaag aaacgttaga agaaggcgca tcaaataaaa agattgtcaa
     1501 actatccgtc aatggagtta ttgcagatac aggtgaaagc aatcttttta gtagggaaca
     1561 atataaccat cagaattttt taacacagct aaagaaaatt caagaagaca aagcggttaa
     1621 gggagtgtta ttagaagtaa attcacctgg gggaggaata tatgaaagtg ctgagattgc
     1681 taaagaaatg gcaaaaatta aaaagctaga tattcctatc tatactgcat ttaaaaatac
     1741 agcggcaagt ggagggtact acatttctgc tggttcagat aaaatatttg ctacggaaga
     1801 aacaacaact ggatcaattg gtgtcattat ttctggttta aattattctg gcttattaga
     1861 aaaacttggc gtaactgatg cgacgtataa aagtggtgca ttaaaagata tgatgtcacc
     1921 tcaacacaaa ccttcagagg aagagaataa agttatacag gaatttgtga tgagtgctta
     1981 tgatcgtttt gtcaatgttg tagcaaaagg aagaaatatg gatactaatg cagtgaaaga
     2041 acttgcggat ggaagaattt atgatggtaa ccaagcagta gaaaatgggt tggtggatca
     2101 aattggttac tctgaagatg cattagattc cctaaaaaaa gagaagaaat taactgatgc
     2161 gacggttata gaatataaaa atgacacaac tggctttgct tcttcttggt taggtaataa
     2221 gattgctgaa tggcaagggc taaaggcaac tacatcagat cgtgtaattt cagtttttga
     2281 aaaacttgga ttttctgata ctccaaaacc aatgtattac tatggagggc tataaaatga
     2341 ataagaagag tggcattgaa actttgccta attatgtatt tgcgggattt tggttacgat
     2401 tttttgcttt tcttattgat ttaatttgta ttagcatcat cacccatgct actataggtc
     2461 tcttttacaa aatcatggga ctggaatctg acaaatccat tattactttt tatggtatag
     2521 cgtcattagc tatttattta acctattttg tacttttgac taagctgaat tcaggacaaa
     2581 caattgggaa aatgattttt gggataagag tagtgagctt tgaggaaaaa gagctaagtt
     2641 ggcaaactat ctttattcgt gagtgtatat gtaggtttat attaaaaaat tttgtatttc
     2701 aaattgggta tttggtggct gctttcacgc ccaagaaaca acatattggg gattattttt
     2761 ctaacacgtc agtggttgtt attcgttcat tagaagctat taactatggt caaaaaattt
     2821 ctaattctaa aacacttaat ctggagagaa ttaaatgaat aagagaaaaa cagagtttat
     2881 tttgatttta attagtggaa taacagggat tttatgtagt cttttagcat tgatgggtgg
     2941 tatgtttcta tcttctattg attcaactac tttaaatact acagaactaa ccagtaagga
     3001 tatagatatg ttagtcaatc taagcgggaa tgttgtttta tttggaggaa ttaccctagt
     3061 tttttcgata atgattttca taataggttt tttagtaaga aaaaatcaaa aaacagtggt
     3121 ctatggtatt agtttactca ttttatctat tattccattt tttcttttaa cattattgtg
     3181 ggtaattcct ggagttttag gtattattgc gggattgatg cttctaataa gaaaatctta
     3241 attaggcaat gtgtagacaa cgtgaaacga ttaatcaatt aaaagctaaa tacgttttga
     3301 gaaatgataa cttgtagtcg atggaagaca tgtagataaa aaaactacgt gtcttttatt
     3361 tattctaatt ttttcgaaaa aaagacactc tctgattgtt gtattaatgg aaaaggggaa
     3421 agcagcaagt aaatttagtt tttctattaa atattcttca ctttttaacc ttttatagta
     3481 cttagattta atcctatatg tttagagagt tcattggcag catgattttg caaagcttct
     3541 cgaattattg ttatattaga aactttactg ttcataatta tgcttctttt ttaactcaaa
     3601 gtccaaaggg ctacagtgga ctttgagtta aaaataaaaa gccctagtta ttttgaaaaa
     3661 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
     3721 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
     3781 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
     3841 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
     3901 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
     3961 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
     4021 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
     4081 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
     4141 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
     4201 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
     4261 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
     4321 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
     4381 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
     4441 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
     4501 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
     4561 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
     4621 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     4681 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     4741 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gttccagaag
     4801 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     4861 tatatacaga taaaattgac attacccctg aagagatcta tcaacgtttg ccagaggaaa
     4921 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     4981 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     5041 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     5101 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     5161 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     5221 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     5281 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     5341 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     5401 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     5461 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     5521 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     5581 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     5641 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     5701 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     5761 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     5821 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgacgttt
     5881 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     5941 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     6001 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     6061 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     6121 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     6181 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     6241 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     6301 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     6361 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     6421 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     6481 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     6541 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     6601 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     6661 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     6721 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     6781 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     6841 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     6901 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     6961 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     7021 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     7081 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     7141 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     7201 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     7261 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     7321 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     7381 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     7441 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     7501 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     7561 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     7621 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     7681 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     7741 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     7801 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     7861 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     7921 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     7981 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     8041 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt gattttaagt
     8101 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     8161 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     8221 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     8281 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     8341 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     8401 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     8461 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     8521 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     8581 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     8641 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     8701 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     8761 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     8821 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     8881 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     8941 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aagaagtata
     9001 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     9061 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     9121 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     9181 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     9241 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     9301 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     9361 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     9421 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     9481 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     9541 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     9601 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     9661 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     9721 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     9781 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     9841 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     9901 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     9961 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
    10021 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
    10081 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
    10141 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
    10201 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
    10261 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
    10321 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
    10381 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
    10441 aagtggtcca aattgagagc gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
    10501 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
    10561 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
    10621 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
    10681 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
    10741 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
    10801 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
    10861 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
    10921 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
    10981 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
    11041 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
    11101 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
    11161 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
    11221 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
    11281 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
    11341 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
    11401 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
    11461 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
    11521 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
    11581 gtactgtcgc tagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
    11641 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
    11701 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
    11761 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
    11821 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
    11881 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
    11941 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
    12001 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
    12061 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
    12121 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
    12181 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
    12241 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
    12301 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
    12361 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
    12421 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
    12481 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
    12541 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
    12601 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
    12661 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
    12721 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
    12781 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
    12841 gcggtttttc aacatttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
    12901 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
    12961 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
    13021 gtcttgcaag cgaattcatt agcaaatgca gaaatcttag cctcaggaca agagttgacg
    13081 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
    13141 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
    13201 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
    13261 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
    13321 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
    13381 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
    13441 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
    13501 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
    13561 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
    13621 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    13681 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    13741 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    13801 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    13861 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    13921 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    13981 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    14041 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    14101 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    14161 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    14221 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    14281 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    14341 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    14401 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    14461 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    14521 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    14581 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    14641 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    14701 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    14761 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    14821 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    14881 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    14941 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    15001 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    15061 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    15121 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    15181 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    15241 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    15301 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    15361 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    15421 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    15481 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    15541 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    15601 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    15661 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    15721 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    15781 cgatctaggt ttagaattga aagatgcaac aatcgaaaac ttaggaaatg cttctaaagt
    15841 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    15901 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    15961 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    16021 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    16081 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    16141 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    16201 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    16261 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    16321 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    16381 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    16441 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    16501 gtaggaatta aaagagaacc cttatattac agggttctct tttctttttt tgttttattt
    16561 taagtaaaag gggcaaaaaa ggggcagaaa tatcaaagcc tatttagttt atcaattact
    16621 tgttgttttg cttttttggt tacgtgattg taaatagaca gagttgtatt tgcatcagaa
    16681 tgtcctacac gctccataat ggctttaaga ggtacgccta attcagataa tagcgaaaca
    16741 tggctatgtc taaatatatg cgatgataga tttttttcta attccaactc ttcttctact
    16801 ttatggagta cagcgttaaa tgagtgcagc gtaagcggag tgccatttgt agatataaat
    16861 atatattgat cgggattggt gggtctacct gcaagaatat tgtcagctat cacactttca
    16921 atcaattctt ttgcacgatt ggataattgt acttcacgtt gtgagtaagt attttttgga
    16981 gttgttttta tggcgttatc catttttaca gatgtgtaat ctaaggtccc attaatggaa
    17041 attttaccct ctccatagtc cttcatttgc aaagccagca attccccata tctcaaacca
    17101 gtcaaatata gaaattcagc tattatgccg tgtagttttc tgcgaggatt ggaatataac
    17161 tgtttcagta tttgatcaat ttcttctttc tccagatatt ttttatccat agaaagcttt
    17221 cttttttctt cttccacttt tttaggatgg attttaactg ctagagcagg gtttctttga
    17281 atgtattttc tatcaattgc atagtttagc ataacggata gagttgtttt tgtttgcttt
    17341 gtgtagttca gtgagagatc accaaaagtg tacatatctt caatcatctt attaatgagt
    17401 gtctcatcaa tgtttctaac gattgtatca tcgcttatgt gcttagaaac atgtttcatc
    17461 atcattggaa ccttcaaata gctagtacgt ttaacatgct gcttataata ttcataccat
    17521 tctttataca gctcaccaaa agtgatatct gatttattgt aattttcgag tgcttcttta
    17581 atttttttat ctagaatttt ctgagctttt ttccacgctt gtggtgaatt acttgtaagt
    17641 gttgtagatt tttttcgtgt tttttctgta taaggatcta catatctttc aataaacttg
    17701 aattggccat ctttggtttg ttcaacccac acttttaaca tctcctatca tttgctataa
    17761 taggcataac aaatagacct atataggttt gttttctaaa agcacgctct tactctggac
    17821 ggtggggcgt gttttttatt tattatacag ttgctttaaa aatagctgct ttttcaaatg
    17881 atactaaagg agagaagtgg atttctattt ctcccttttg gtttaacgtg aaatgagcag
    17941 ttacatccat ttgttttcct ggagcaacag atcccattgt attttcgttg gcatatgtct
    18001 ctgatttttt gtcatctggt ccatatactt ccacatctgt acctacaggg atatctgaat
    18061 cgccatcatt ttttacggta taagtaattt ttactacttg tgcaggttgg ttttcttcaa
    18121 attgatttct ttcatcagtt agttctacac tatttagtgt atattcagca tcaccaactt
    18181 ttacagtgtc accaatctta tagaaggtat cgcttttttc ttctttagaa gatgaagcag
    18241 aggttgaaga tttagttact ttttcgccac cattatcact tgctttatta ctatctgatc
    18301 caccatttaa agcagaacca ataattataa ttaaaattac tgctaatacc caaaaccata
    18361 cttttttgta aaaaggttta ctaactttat acatttttcc gtcttgaccc ataacttttt
    18421 ttgccattta aatattcctc acttcttgtt ataatatatt tgcgatctca gaaatgaggt
    18481 atgagtccgt gtgccagcac gggctttttt aatgttttgc tgaaatcggt ttttttaata
    18541 attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta aaatagcatt
    18601 ggcagacagc ataacaagtg tatctgagct tattctgaat
//
LOCUS       DAZULG010000056        18522 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000056 DAZULG010000000
VERSION     DAZULG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.057"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACG9CY_001675"
     CDS             complement(<1..272)
                     /locus_tag="ACG9CY_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938185.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="ACG9CY_001676"
     CDS             complement(309..446)
                     /locus_tag="ACG9CY_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4938186.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..879)
                     /locus_tag="ACG9CY_001677"
     CDS             complement(448..879)
                     /locus_tag="ACG9CY_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938187.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(893..1387)
                     /locus_tag="ACG9CY_001678"
     CDS             complement(893..1387)
                     /locus_tag="ACG9CY_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938188.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(1387..1836)
                     /locus_tag="ACG9CY_001679"
     CDS             complement(1387..1836)
                     /locus_tag="ACG9CY_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938189.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTMVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(1840..2457)
                     /locus_tag="ACG9CY_001680"
     CDS             complement(1840..2457)
                     /locus_tag="ACG9CY_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938190.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(2457..3365)
                     /locus_tag="ACG9CY_001681"
     CDS             complement(2457..3365)
                     /locus_tag="ACG9CY_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFS4938191.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(3378..6137)
                     /locus_tag="ACG9CY_001682"
     CDS             complement(3378..6137)
                     /locus_tag="ACG9CY_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS4938192.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMDVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(6134..6838)
                     /locus_tag="ACG9CY_001683"
     CDS             complement(6134..6838)
                     /locus_tag="ACG9CY_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4938193.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(6850..9159)
                     /locus_tag="ACG9CY_001684"
     CDS             complement(6850..9159)
                     /locus_tag="ACG9CY_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4938194.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(9354..9818)
                     /locus_tag="ACG9CY_001685"
     CDS             complement(9354..9818)
                     /locus_tag="ACG9CY_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938195.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(9818..10444)
                     /locus_tag="ACG9CY_001686"
     CDS             complement(9818..10444)
                     /locus_tag="ACG9CY_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4938196.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(10451..10816)
                     /locus_tag="ACG9CY_001687"
     CDS             complement(10451..10816)
                     /locus_tag="ACG9CY_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS4938197.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(10806..11144)
                     /locus_tag="ACG9CY_001688"
     CDS             complement(10806..11144)
                     /locus_tag="ACG9CY_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938198.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(11134..11460)
                     /locus_tag="ACG9CY_001689"
     CDS             complement(11134..11460)
                     /locus_tag="ACG9CY_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4938199.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(11441..11719)
                     /locus_tag="ACG9CY_001690"
     CDS             complement(11441..11719)
                     /locus_tag="ACG9CY_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4938200.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(11721..13085)
                     /locus_tag="ACG9CY_001691"
     CDS             complement(11721..13085)
                     /locus_tag="ACG9CY_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4938201.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(13098..13667)
                     /locus_tag="ACG9CY_001692"
     CDS             complement(13098..13667)
                     /locus_tag="ACG9CY_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFS4938202.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(13639..14826)
                     /locus_tag="ACG9CY_001693"
     CDS             complement(13639..14826)
                     /locus_tag="ACG9CY_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4938203.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(14890..16617)
                     /locus_tag="ACG9CY_001694"
     CDS             complement(14890..16617)
                     /locus_tag="ACG9CY_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4938204.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(16614..17066)
                     /locus_tag="ACG9CY_001695"
     CDS             complement(16614..17066)
                     /locus_tag="ACG9CY_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS4938205.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(17178..17558)
                     /locus_tag="ACG9CY_001696"
     CDS             complement(17178..17558)
                     /locus_tag="ACG9CY_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4938206.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(17555..17941)
                     /locus_tag="ACG9CY_001697"
     CDS             complement(17555..17941)
                     /locus_tag="ACG9CY_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFS4938207.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(17943..18221)
                     /locus_tag="ACG9CY_001698"
     CDS             complement(17943..18221)
                     /locus_tag="ACG9CY_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938208.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            18284..>18522
                     /locus_tag="ACG9CY_001699"
     CDS             18284..>18522
                     /locus_tag="ACG9CY_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938209.1"
                     /translation="MLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGVSSYSA
                     FDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEG"
BASE COUNT         5145 a         3994 c         2859 g         6524 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataatg gaacgaaaac cattacttgt tcgttagtga aacagcccca gtcatacatt
      421 agtttgatat cttcatatga atacatgtta cttcccacct tgtaattgtt ttttgatttc
      481 ttcgatttcc ttggtatttt gtacagaagc aagcatcgtt tttgaattca actgcgctaa
      541 actttctgac ttttctttca ataagacatt ttcttgcttg attgctacat tgtttagcat
      601 tgctttagaa tttagctgtt tcaattcatc attttctgcc ttcagcgttt cgtataatgc
      661 cttaatatta gccaattcat ctacgctatc aacagcattc ccttttttct cttcttcagt
      721 agctaactca acccactgtt ttttatcaaa atcaaatctt ggtctccagt taggaactgg
      781 tggtttaatc tctgtacagt tttcagggat attttcttga ttgttcaaaa tgatttgctc
      841 gaatccgtaa ggcttgattg atttgtaaac tactttcatt agatttcctc ccttaaattg
      901 aataagtgat aacaaatgaa taagctgatc cataacttga atttcttctc catttaatgg
      961 ctccgtctgc gccaatagat agttgagcac tgttcaaagt agaacggtct attgacccaa
     1021 ccagttgttc aaaactaatt ggtgtccgat agccttctgg aacagttaaa ataaccgaat
     1081 cattcccgcc actacttttt ccgtttaaag ccacaaaata tatagaaact gtttttcctt
     1141 cacgataaag ctttgctgtc cctgtattac cgttagtgac aaccagattt accacttcat
     1201 atctgttatc atcagtcgtc aaaacacgtt tattattgat tgttacagaa cctgaaaaat
     1261 tcttatgtcc tgcaatggct tggtctccat ctgtctgcac caatgcttct ttaccatcaa
     1321 tagcttctac atgcgttttt aagtatttag caactccgtc ttcttttaat tgaacaatat
     1381 cagccattaa accgtcccca ctttctcaaa tgtaatattc gctaatccat caagtttcac
     1441 tttatcggtt gctgacatta aacctgctgt cgtagttgta gcattacctg gatttttctg
     1501 tgctccagct gcaattccat ccaacttagt tttatctgtg gacgacatca acccatttgc
     1561 tgtagttgta gctacagctg tagttgtggc atttattcca ggatcacctt tatctccctt
     1621 tggtaaaaca aaattaaatc tagctgcaga tgatgttcct acattcgtaa cagaagcggt
     1681 tgaaccacta gaaacggttc ctatggtaat tgttgctgct tggccaggat cgcctttatc
     1741 tcccttcacc attgttggtt tgccttctat agcattccaa tgagtttgtg gataaacctg
     1801 tacattgctt tgttttattt ttacgatatc tgtcattctc tatacctccc caattttttc
     1861 aaaagtaaaa tttggaattc tttcgtttgt gtaattttct gcttgattta cagcttcttg
     1921 gaatttttga tctacatatg actgattaac accaccagtc ccactaccac ctgtagaact
     1981 aattgttcca tcttctgcaa ttgatatatt tgcacctgct tttaatattt ttagagattc
     2041 taattttcct tttaattctt cagagaaatt gaagtctgtt tgcttagttg cagataaaac
     2101 accttcttca gtaacttcta agagttcccc aacttttata cctcctaatt gttcaggagt
     2161 agcaatcggc aaaatatatg ttccaccttc tccatttacg atcttttgaa acatttcagc
     2221 agtaagaata ccatcgcttg tctcgcttgc ataaggtagt tcagtaattg cattctctaa
     2281 tcctaaatca gctttcgtta aaatgactgc accatattta ccattaaccg aaagaacttt
     2341 tgattgtcct gatatcattt tttctaagcc taaaactgca gagacatgtg taattggcat
     2401 aaattgacgt ttaacaccag attcatcagt ttccatcatt ctctttactt taaccatcaa
     2461 atcaccccaa ctttctcaag agtgaaaaca ttctgttttg gatcatcaac agttgcaata
     2521 atcaaagctc cttcttcaat tggaaattct actgtttcaa ttttttcgac ttcgtgatta
     2581 tctgaaaaga gatcgtcttg aagaatatca gtcacttcaa tttcaccgta ttcaatcgtg
     2641 aaaagtgctg ctttcatttt ttgatacaaa taatttagat cggataggaa acgttcagaa
     2701 atcgattcat ggcgtacacc ttgaagatct acacgtgcat ccattaattc ggctaacatt
     2761 actccacctg gatcgataga cttcaaaata tccttgattg attcaaacca tgacaagtaa
     2821 tctgattctt gtccttctct ccaatcttgg aaactattct cctgttcctc tctccagcga
     2881 tcaaattctt ctttcctttc attcatccag tccgtaaaat cgcctttatt ttcattaata
     2941 aaatctgtca tatcagcaat taaatcttca attgattgcc aataagaacc catttctcct
     3001 tctgtttttg aaacagcatt gatgacaaaa taagagaagt cttgagttgt tccaatcaag
     3061 tcttctcctt taaatataat gaaattggct gtttgtcgat gcaaacactg catggaatat
     3121 ttatcaaaaa tatatttgat tttccctttt ttagcatcca caatttttgt ttctaattgg
     3181 actggatatt ttccgccaac aactgattca aaatatacct tacattctga taaatcatat
     3241 ggaagaccat tttcgacaat tgtagcttcc ataacttcag tgtttttatt gccttgtcga
     3301 acttgaatca tccccacgta attatagggt tctgttgtac ttaatatgac attccacttt
     3361 gccattaaat cacctcccta ttttggtggt atgacaatgg aagaaattgc acttgcacta
     3421 taatactgtc gatctaactt tccacaaatc atacctaact ctgtgttttg ttcataagtt
     3481 tgcatacgac cattagctaa gcctcggata acgccagtat gtccataagt tccatctgca
     3541 aaccatgaac ccacttgtcc acctcttgcc caattaataa ttgcaccaac tactaattga
     3601 tcatatctag ggttttgaat cactttccaa ccaacagcag accaatcata ggcaatacca
     3661 atatcagatg ctgcagaagt atttcctatc acatgagtta aaccatattt tgttccagca
     3721 cccatgccac aaccacccag atatcctgaa tattctgcag acaagccata acactgacca
     3781 ttgccaattc gttgtccaat caaagactct aaatgtttta gtcctgcttc tccagtagct
     3841 cctcctggtt ttaaatcttt aaatttgtta taccagttaa ctgcataatc ttgacgttca
     3901 ggatgtgtcg ctgccggacg ttcatagttt cgttcaaaag catatgctgc ttgtcttgga
     3961 tcagtacagg ccttaaatcc atcaaccgtc gtaggttgta ctacgtccat ccattgtcca
     4021 tttgtaaatg tccaaataag caatcgaact tgtgcatcta agctcgttat tggttcttta
     4081 atacctgcag cattaaataa gttttgaaca taaacttttc catcccatgt tgctggacca
     4141 acaagaggat atgaagaacc atcccactga actaatccgt atgctggtcc acctatttga
     4201 acagtatcag gatcaaaagt tcctcctgtt tcttgttgaa tgttccccaa tattccacat
     4261 gcagattgtt tcgtaaatcc gttattacac aaaatatcgt atattctcca agctctcttt
     4321 tctgcatctg tttttaattc atcaggatac ccaccaggtg tttctcctcc gccagatgga
     4381 ccgccatttt gtcctggtat cacttcttta cctctaacag taagttttcc ctgaatatct
     4441 aaatcaccga aataatatgc ttttccgttt cctaataaca caaatccttt ttctgcagtt
     4501 ggagaaatta aaatgtattt tccatcgcca tttgtacgta ttaccaaaga attttcctca
     4561 agaggtgtag gtgttacagc atttggaaat ggatttcctg ctgaatctgt tgttccgatc
     4621 gttccaatgg attcttttct attccagaat tccatacctt ttttggttag ctccatgatt
     4681 tttttgtttt cattccaaat ttgaagcgtg ccttttacaa gttttaacac atcaccataa
     4741 gcattaaagg aagtttcgaa tacttctgca tttattgttc ctactttgat gaagtcagca
     4801 acaatttctc cttttgatgt gatagcaata ccgaacgggc catttactcc gttgtctgaa
     4861 taacctaagc catttaaatt ccaacgccac actcgctttg cattcgccac atttggagta
     4921 tccatgataa gaatctctga tggagctttt tctggacgaa aaacgacatg cccaccagaa
     4981 ttaccagtga tccatgccgt cgcattcaac acattttgaa ctaacgtttc tgttcgattg
     5041 tcgattttct ttttcaattc ttgtgcttga gtatttactg ccgaagtgta cagtgataaa
     5101 tcattaccta aaacaatatc cttatatttc ccaagcgttg ggaaataagt gtattcgacc
     5161 atacgctctt ttatctcgat atctagctct ttcgccctaa catgagcgac atcaccaaag
     5221 tgtaaagacg ctagctcttg atacatgtca ccgtattcga gtgtgtgttc caacgccacc
     5281 atactgactg tatgagtagc ttttggctca tggatacgat cattgtcaaa taaggttttg
     5341 ccccatttga tcaattcatc aactgtttta caatctccgt tttcacgttt ggcaatacgt
     5401 cgattctcgt tggttactcc atcaatttct aaataaccat attcgatagg ttctttgtct
     5461 tcatcataat cgttatcagg aacaccgcca accaaataca aactattgac aattgattct
     5521 tcatcaattt cttcttcgat tgcttctaaa ttgatgccaa aatcaatccg aaaaccatta
     5581 tctgatccaa tctgttttac cagtttcaaa tcatagttat ccatatcaag ttccgcgcta
     5641 gtcacgccag tcaaattttg attaccgtta tttgaaccaa tgattgcttc aactggcgcg
     5701 acttgtcttg ctgtaaattg gtgcgttgtc cctacattcg aaagatagtt aaatttttga
     5761 tcaaatgcca aactgttttt aagattggtc atgatctgag acccattgcc gttatccgta
     5821 aacgacttga caataaagtt cttattggcc ataaaaccaa tatgccgagc tgttacagaa
     5881 acggaggtca aattcttttt gacattataa atttcaaaat attgccatga tccatctggt
     5941 accttcgctt ttataaaatt tccttttttt aaataagagc gatactgtcc gcttcttgaa
     6001 tagttaccat aaaaacgata ttggccattc agcacacgat tgatttctgg caaatcttcc
     6061 caatcaacta acgacgctcc gttcacgctc aaatcatctg gcattttttt ataagcataa
     6121 ataaattctt gcgtcacaga tacacactcc tattccaaaa ccgcacttct ttaaattttc
     6181 cggatatttt tacctgattc cattcaggct gcaatactgg ccaatcacca cgcgtaaata
     6241 aattcaaacc ttcttgtatt gcttttccaa gctgagtatc aaccacgatt gttgcagcta
     6301 acgtattaag tattgttagg cttttatcac cgacagcgat cgttatatct cctccgttcg
     6361 attctatttc cagataggga tgtgcaattt cgtcaccatg atcaaaaata tccataacac
     6421 tagaatgaaa aattttaggt gcttcaccga tttttctttt gagtggctga caacgaaaag
     6481 taacatcgaa tgtataaaag aagccccact cgttttcgaa agggacttct ttatccatgc
     6541 tgcagatagc ctctagatac ttgtctggat cattatgtgt gattaattgg cttttaccag
     6601 tgagccatcg tttgacttct ctcagtttcg aatgtggaat agtgatccct tctatttcca
     6661 aatcaaaagg ttcatagtca tcaaacgtct ccgtcaattc tccgcttcgg ccctgaatcg
     6721 tgtaagtttc gtatcgcttg ttcggcataa tatctggaag ctctgcctca atgatgcaat
     6781 tcatatcaat tactgcattc cgatttttcc aaataaaatt tggttcatct ggattcataa
     6841 attgtcgact caaataggaa cacctcccaa atcacgaata gcctgtttgt ttgctttagc
     6901 aaatttacga ttcatacgat ctaactcaga tggattgttt gcatcgactt tgccaatgtg
     6961 tatatgctgt tcaatgctac ctccagaaac tcttccactg atccctttgc gtttttcttc
     7021 gtctgataat ggagtaactg tggttttacc attcttggca gtcagtaact caggtccagc
     7081 ttcaccaaca atcgcttgtc cattgattag atgaccgcct tgtgctaaat aaggcagttt
     7141 tgaaatagaa aatgattttc cacctacacc aggaacccat tttggtatct ttatattatt
     7201 caatccacca ataaatccat tgatcaatcc aatcatggca ttaataggag ctttccctac
     7261 agcaacaata ccatcgaaaa tacctccaaa aatatcaact agaccttgcc atgcttttga
     7321 ccaattccct gtaaatactc cagtgataaa atctagaaaa ccactgaaaa ttcgttttcc
     7381 agcattataa aaattattga aatttgctat aacaccatcg aaaaatccac caaaataacc
     7441 acttaaaaaa ttaaatattt caacagctac atctgaaaca cctttgaaga aagaattcac
     7501 tccatcatga aaccatttaa cgttattata tgcccaaatt agtccagtaa ctaatgcgcc
     7561 aatagctata actgctaatg caaaccagcc tcccgacatt ccaaataaac cagcaagtcc
     7621 ttgccaaact cctataaaac ttttaacttc cccaacgatt tttgtaacag atcccataag
     7681 tgaaccaaga acgattagta ctggtccaac tgctgccgct ataccagcaa ttgttatgat
     7741 ccaattcttt gtatcttgat ctagagaccc ccaccattta gagaattcct gtaataagga
     7801 agttgctttt tcaaaaaaag gcaaaaggct tacttggact gcttcaccta cgtcagccat
     7861 tgctaatttt gcattgttca tagcttgatc ggcttgatca attgggtcga gagtagcatc
     7921 gaatgtgtct cctacagctc ccccactttc tccagcagtt tttgctaaat cttccaggtt
     7981 caaagtacct ctacgaattg catcggccat tctaggaccg cctttagtcc cgaatacttc
     8041 tgctgctgca ttgatggctt ctgtttcaga acctgcattt tttaccttgt cttgtagttc
     8101 ttctagtcct tgacttaaag attttccgtc tttcgcataa gccactgttg ctttagataa
     8161 actacttaaa gctgcgcttg aatcaacacc agattgctca aattgtccaa gtaaagtgac
     8221 cccttcacca aagctcaacc ctaattgttt aatttgtggt gcgccatcaa ttgctttttg
     8281 catcaagtcg tctacagatt gaccagtatt ctgcgatgtt ttcgttgtta catcaaggac
     8341 agaattcaaa tcatcatatt ctagtccata agcatcaata gcttgtcgag cagatattgc
     8401 tgattgcgaa acatctgtat cattgatttc agcgtactgt aggagataat tggttgaatc
     8461 ttccagtttt ttatccatga atccaaattg tgtatttacc tcaccaatag cttccccaac
     8521 cgtttgtaaa ggtaaatgag tatttgaacc aacgtttttg aaagactgtg aaagtctatc
     8581 agcttgatca cctgttgctc cggtttttgt aatgatggta tcaagtgcct cgtcaacttc
     8641 accaaatgct gcaagtcctg ctgctcctgc cgctacaata ggcgcggtta ctccaacaga
     8701 cattttttca ccaacgcctt ttactttctc acctgtttct tcgattttct ggagtttttt
     8761 tgctgtatca acagaaacgt caccttgttc tttaagtgca tcgttcgtct gttctaatgc
     8821 tgtacgaagt ttattttctc cggtttcaga attgagtaat tgtttataaa gtttttctga
     8881 ttcagcagaa tattcaccgg tttctttgac agatttttca tattcctctc tcagcatttg
     8941 agatcgttgt tcagccaatc ctaattgttt ttctaatttt tgtttagccg ctcgcaattt
     9001 ttctgtttga gttgcatcct tgtccatcgc agacacttgg tttttgtact cggcagcggc
     9061 taagttcatt tctttgttga tttctttgat tgttttcgaa taactgactt cgccattcgt
     9121 tttaaaatta agaacgacat cagattcttt accagccatt tatcttagcg ctcctttcta
     9181 ccaccaaggc gatttatcca tagttacaga ttgaggtggt tcaaaatcgg tgttttgttg
     9241 taaccactgt aaataagatt tgagccacaa gttaggtgta gactttaaaa agaaactctc
     9301 actccaattc aatagagtga gagcaacgta aatataaaaa ctccaaggag ttcctatctc
     9361 ttccgattct ttttgttttt ctttcttttt gcttgcgctt tttgaaagtc ttgtggcttc
     9421 ttggattttt ttaggtcttc cacctgaaat gtctggctag caaaaatttc catacaggta
     9481 ctataagcag acaacacctc tccactcatt cccaaaaatt tgaaaatagt ttctggatct
     9541 tcctctaaac caccagtacg cagcatggca taaatcaagg cacgcatgat ttttagatca
     9601 ctaggcgata agttagcaga agaaatttgt ccttcttttt tagatagcat ggcgttcata
     9661 tcagattcaa attgcgaata atctccacca tacacatctg ctataaattc catcgtttgc
     9721 atagtaaatg aaatagggaa ttctgcgcct tgaataatta caacagcaga attttttaag
     9781 tcttcaacgt gaattccata atcagataac cgagccatta acccgcacct ccaactttag
     9841 ccaatgtttt ccactgttct tcatcgtata caggttgagc aatgaattta ttaaataatt
     9901 ctagagaagc tccgtctcta tttgaatcaa aacttgcata catcacatta ttgtatttca
     9961 gtccgatgga aaccagattt gcagttacat cgtcaatttt tgtttcttcc tctgcagtgg
    10021 catactcttc gtcaatcaca ttggatagtt gtgtattagg ataccaaact gcttttttac
    10081 cgccattttc aatatttcca ataaatccaa aggcgaagta cggaaattct cgtgcggtat
    10141 ttttgctaaa cgttactccg cttttagcaa ctaatccttt gatttcatcc atcacttcta
    10201 tcggaatacc cacgtgatcc aacgcaagtt catgttttgt ttctcgcgac acacgacgaa
    10261 acattttgct ggatgcccat ttttctaatg ctgttccatt ccctttgaca ccgatttttg
    10321 tggcaattgg caatcgtaaa acttcactat aagtcggtgc agttccaaca gaatctggcg
    10381 ttgccatcat cgcaattaaa atgtcgtcca atccttcaaa ataaaaagta tcttgttttc
    10441 ccaattaaaa tcacccttcc cataaatcaa gtatttttct agtcataatt tcttcaattt
    10501 tttctttgtt ttgttcatac gtaccacttg caaaatgttg ggcacgttga tttgttgtgc
    10561 cattttcggc aaagcgccag taaaaggccg tctcttcaaa ttgaactttc acttgatcgt
    10621 tttcaactac tacttttatt tgatcgcgca tatgtttctt ttttaagagc gacttaggaa
    10681 tattaggaag caactgcttt agataaaagt tagctgcttc ttctaaagat tccaacgata
    10741 attttttcgg atcaactttt gaaaggtttc ctaaataatc cgatatttca gaaaaaccat
    10801 tcttattact tggcattttc cacacacctc acatatgtgt aatagttggt tacggtatcg
    10861 tcgttctcat caccttgtat acctgtaaaa tctgaatatg gaatgccagc attttgcagc
    10921 gcttgttcaa taacaactaa atcctgttct gttccaagtg taaaaaaaga gacttggtaa
    10981 taaggtaatt ttttatagac tttaccggat gccatttttt tgctgttact cacatttgag
    11041 tacacaatgt atggatacat cgtccctaat ctggctttgt ctctgaacac tgctaacttt
    11101 gttgatttca gcgctgtttt caattcatca aagctaatcg acataagcta aactcaactc
    11161 catttctctt gcatcgggat tcgtataaat gcgagtaatg ttatacgtta cagaatcaat
    11221 tttgagcgca cttaatttct ctgtgatgga tttatcccat ctgactttaa ttcgtctgac
    11281 aacgtctgtc ttggcttgct gtgataaata tttttcttga gaagtcacac cgagttcttc
    11341 atagaatatt agacgcttaa attcgtaaat tgtagttgga cgatcgtttc cgtctgttcc
    11401 tgttttgatg tctagcaatt cggctttcca tctgagatta ttagtctgtc tcttcggcat
    11461 tttgaatcgc tccttgcacg ataaatggcg tcatggcatt catagctttg tcgagttcat
    11521 cctctgaaac tctatattca taggcaatgc cggcaaccat caaaataaga tattcttgtt
    11581 ggccaccagt tgctgttttg acataatctt ttgccatatt taaataaaaa gagagcaaag
    11641 aatcatccat gccctcttca aaatgaatat gtgatttgaa tttttcctct aaagacaatt
    11701 ctttagtttg ctcttccatc ttaaccacca actggttttg taatttcgta gcgatatact
    11761 gccggttcaa atggagaata aaccaattga ccatctagca agttgtaaat ttggaatcca
    11821 atttgatttt taccagagaa tttttcaaca agtttttgaa tttccaaggc accaataact
    11881 tcttgaattt taaatgcaga aaaatcgcca aaatataaaa ctggcgtgtc tggttcaccc
    11941 tttttatctg ctgcatctgt ccaatccaca ggatagccaa ctaattggta accaattcca
    12001 ccttctgctt gtgtgaatgg ccgcaacaaa ggaaatccat catctgtttt cattttttca
    12061 atggcagtca aagcagctcg attaataata aagcgtccct ttttcatcac ttctgtcact
    12121 ggtgtatttt taaattcgat taatgcatca tataattttt gcccagcacc tgaagcagtt
    12181 agatctaaag gttttttaaa tgctacagcc tttttggcta atgcaccagg attttcattt
    12241 ccagcgtcat caccattgaa catataattg atttctttac gcacataagc ttttttcaat
    12301 tcttccacaa caatatcttc aactggaaca ccagacattt ttaataattt tttagttact
    12361 gttgccaaag catcgaattc ggcaggatta agcaaaattt catcaaactg aatagctgtt
    12421 tcagcaatat cagttgaacg ctctttcttg tttacattcg catctgcttt cttcacaaga
    12481 attggatatt tgacatctcc tgatgttcgc accactgttc cgtatttacg aagtaaattt
    12541 tcttcttgag cataagtaat aacttcagat gcaattactt ctgggacagt aactgaaccg
    12601 ttgccagctt caatccctaa agctcgagct tctgcttcag aaatatttcc agctacaaaa
    12661 ttagcaaatg cttttcgtag ttgttgatct ttttgtttct tggtcatttt tgcacgtgcc
    12721 tccaatccgt tcttaattga tccaagtaat ccatctcgtt gctgttgagt aatcattcca
    12781 gaacgatttt ctgaactatc ttcactgtct gaatcttctt cttgatcgtt gtcttcacca
    12841 gaacggcttt cgtctgaatc cgtactattc gattgatcat ctgtattgtc gtctgtttca
    12901 tcagttccag aatctgcgcc taattcgtct ttaattccgt tcaattcatc aataacactg
    12961 tcaatttctt ctttcacggc ttctaaatct gcttcgcgta attctcctga ttcaactttt
    13021 tcacgtaatt cagtcaatcg ttgctcacga cgtgccttca tttttttcaa taattcttta
    13081 tccatgtatt tttcctccta cgcttctagc gcttgattaa tttttttgat taattttttt
    13141 ctttcttcga cgtttttttc taattcatca cgacttctta atgcagcttc tgtatcttcg
    13201 tatgctggca aaggcacaat agaaacctcg tataattcca cttcatggat tgttcgaagc
    13261 attggttcag aattgtagtc ccacgtttct tcagtcggca caaaaccaaa actacattgg
    13321 ttgatgtctc ctcgttccat agatttaact aagtcccttg ctaccgttgt gtccggtaaa
    13381 tcaacttcga atttcaaacc acgttcatct tcttcaaggc gaagggttcc gcttttagta
    13441 cgtcctagca cattggacca atcgtgattg aacaaacaac gaacatccga attactaatt
    13501 gcctttgaga atgcaccagg aacaatcact tcgcttaagt catcccataa aagtgtttga
    13561 ctgttgaata cagcagcgta gccgctaatg gtcctcgtat gagtttcttc atcggaacgt
    13621 gttgaaagat tggtgatgtc aatcgtgcga atttccttct tcttcatttc catcacctcc
    13681 tttcaagtct tgatcattcg ttggtaagga atcatctgtt gcatttttct ggccaatcct
    13741 agataagtca tttgaaatat agatagcttg tgtttctgga gtattctgtt taggaaaacc
    13801 aagcatttct gccacgttat ctggacttgt aatcccggta cgaacgatgt tgtagccaat
    13861 atttgttttt gttgagtaag gaacaaaatc caaaatatta attttccatt ccactctata
    13921 gccagaatta ggcataaaaa aaagagcggt gtaatgttcg ctcttgtttt tcaatattgg
    13981 tttgattgct ttgttgtgca gatacatcat cgctttttca atatctgtct tcatcagcga
    14041 ttgatacgta tcaacattga ttcctagaaa ttttcctaag tcttttttgt aaacacctaa
    14101 ataattcaaa attgccgcgt catcaacagg actttttaat gtctcgatgg aatatccttt
    14161 tcccaaagga atcatcttaa cagaatgatc actctcattt tgcgttcctt ccagttgatc
    14221 caatatagct ttgacaattt ttgtttgggc gctattattt ggattgatgt gggcgtctag
    14281 tttaagcatg aacgcaagta aaccgccttt agtatatttg tccgtcaaaa ctttttcagc
    14341 gctcagaacg ccttctagag tgtttcttgc aagatcaatt attccagcac cttttaatga
    14401 atcagttccg atgttcttaa tgtgtcgaat catttgacca ggtatttttt gaccattcat
    14461 ttcaaattct tcttgaagtc gttcattgat tttagttgtt acaccgtacg ccaaatgaag
    14521 ctgatcccta tccgttaatg ggaatgtctc accattgatc aataaagtat ttgtttccaa
    14581 tttggtaaat tcgaatcctg tcaaataatc attgggactc ttcaaaatat ttagtaagtg
    14641 atgatctttg acttcgttgc cgtcgggacc aataacaatt ggtgtggcca atgccacctg
    14701 attagatatg tcttgaacta attcataaac atcagaagat tccatgatag atgaatccgt
    14761 cacatatctt tgaccataac gtgtgtagtg tccaaaaata tcctcgatat atccacgttt
    14821 ttccataaaa gaatagactg cattcgataa ccgatcacgt aatttcaatt tttttcaccg
    14881 cctttctatt tatctataga tggaacttag gtaatcatct aactcatctg aatcaatatc
    14941 tgtcatttga ttcatcgttt ccttatgacc acacaaaaac gccacgaacc catcaatctt
    15001 tttctttgat tgacgtttac ttggcgcttt ttgtccattg atgttagtaa ttgctacaac
    15061 gttcaaagtg caataaagga ataatggatt atcaaattga attcgtttct cataaaacaa
    15121 ccgttcgaca tcgtcaaaag gagcattcaa tactttagga tattgagcaa cttcaacgca
    15181 ttccaatcct aagttttcta atttctcaac aagtttgtca ctcatcgctg gatcataatt
    15241 cacttgctga atatcatata aattcatgca gtcctctatg tactgcaaaa tctgatcttg
    15301 atcaatcatt tttccgtcgc aaaattcaac gaaaccttgt tcagccaaat cactgtaagg
    15361 aacattatct tctttttctc gaaactctaa atcttcattc ggaataaaat aaagttgctt
    15421 tactttaaga accgctttac catcttcatc ccatgttggg aagtttaaag atacacaggt
    15481 caaatctcgt gtacgtgata aatctaaacc gatataacat ggttcgccac ttaaatcacc
    15541 aagttcttgt gtggtaacta aacatggctc tacttgatcc tgttcaaaga aattatccgc
    15601 gccgttcaca aatacatcca agtgtttcgt tagaaattca gctttggaat gagctgaccg
    15661 ttgcgctgtt ttaaatgccg attctaaagc agataaatcg actgatattc cccagttagg
    15721 attgcacatt tcccagactt ttctatccgt ccaatcataa tttttatttg gctcatagat
    15781 tagaacaaag tttgaatcat tatcatcacg tttcaacact tcttttgctt cgcgatatac
    15841 acgcattcca acagacgacg aacctttccc agcagttgag atattaaaca tcaatggctg
    15901 tggtaaagat atctgagcag acttaaaatt gtcatactgt tccattttct cctgtttatg
    15961 caattcatcg ttcaatacaa aatatggatt ggatccctcg atattatcaa tattcttggt
    16021 ttgaacaata aatttatttg agtaagccat atcttcatga atatagtcat aagtaatact
    16081 agaaacagtt ccctttggcc ccctaaatat tttagttcct tcaagtagga ttggattgtt
    16141 taagattgta actgcaaaag gtttggcagc atattgcgct tgagcaaaat cagatgcgca
    16201 agcatagcaa tcaactgata acgccccttc tccgtacatt gcatatccta atgaaccgac
    16261 agcaatcaat gttttaccat tcttttttgg aatctggaca taagcctcac gagtaacgcg
    16321 tacaatttgg cctttttcat tttctttaac ccaaccataa atccaagaat aaataaattt
    16381 ttcccagggt tctaaaagaa acggttttcc caccatttct ccttttgtgt gaacaataaa
    16441 tgattctacc caatccatca tttcattcgc gcgatctaca tcaaaccaaa tatcttttct
    16501 ttttttccac tgataccagc gatctattgc taaacgaacg gtcttaggat acttcttagg
    16561 tttctttctt acttcttttg caaataaatc agcataattt acaccaggtt caatcatttt
    16621 tcagcacctg ccttcttacg ccatttattt ctgtgcgctg ctagttcatc tactggcttt
    16681 acttctgggc gtgtaatttc ttcatctttt cttgcggtcg atcctccagc aattagtcta
    16741 ccagtttttg ctttgtttgt taatcctaat aaatctagag ctttcatctt cttatctgcc
    16801 caagtttcta cttgttgtgc caatggatgt tttgattgat tagtagctcc tgatttattc
    16861 gtgaattttt gcgtctccgg aaatcctttt tccttccaca ataaatattt gtgttggtaa
    16921 atttcaaaaa tatccaagta tgattcgatt aatggatcaa gagtgatggt gtacaaatca
    16981 gacaaattca ttatttctaa gatacgagct ttttcagcac ttactttttc atcaataatc
    17041 gctttacgtt gcgctttagt cgtcattttt gtatacaccc ccctttgttt tttgaaaaat
    17101 ttgacctaac gatgcgcgtg actccccgct accctatctc cccacgcgaa aaaattttga
    17161 aaatgaatag gggggcttca attgaaatac gaaggaaaaa cttttttgtc ttccgtttcg
    17221 ttttcaacaa ttggatgaca ttttgaacat aatagcatga gattgtttgg atcaagctta
    17281 agcagttcgt tgtctttgat tggtacaatg tgatggacgt gtgctctctt tccaaatatg
    17341 aactgaccac accgctgaca atgaccacct tctctttcat aaataaattg acgcatatct
    17401 ttccatgctt gcgttcgata gaatggtttg ttctcatgat gataaacaga ctttgcttgc
    17461 tgcttcttct tgcgtgatct gcttgatctc ttgtgttcag tacagtagat accctttgct
    17521 attttgttcg tgcatccgtc aaactgacag tatttcattc tacttcacga ataagattta
    17581 tgatatcgcc ttttgcacgg atagcaccag gaatatcaat accatgtttc ttagcatatg
    17641 cacgcaattc ttttgcagtc atattgtcca gttcatccgt atcttcagtg gactgatcat
    17701 tagtagcttc gtctccatca aaattagcag cagtgtttcc atcgctatca agaatttcac
    17761 tgctatgagc tatgagttcg ccattgacag caacaaatga tttgccatca ctcaaaattc
    17821 ctttttcttc ttttacttca aaatttggtt cttgatcttt cggaacaact actgtccttt
    17881 tcttttctga atcccaatac tctgttccac taatggatgt tctaattttg atcattgcca
    17941 ttttaattct ctcctttgta atttgtacgg attacttttg cgcccattcg ctcataccat
    18001 tcaatcgttt cctgcagatt gggtagagtg tgtgacaata aagaaataac taacgtattc
    18061 attccagttc tgtaatcatc attctccact tgcacatgtg catgattacc attccaattt
    18121 tctctgtacg gactattctc tgcttcaatt gctttcttat aaactttagc caaacttgat
    18181 tctacttcaa tactaagtac cgcttcatga aaatcattca tttgtttact ctcctatcag
    18241 atattttgtg gtatgattta taaaaaagat gcgaggtgtt ttaatgcttc aagaagttaa
    18301 tattaagtat cgtgatcgtc aaaatacaga gacaatttat ttgccaaata gttgtccgca
    18361 ttgcggaaat ataatgactc cgtatgtgta ttttggggtt tcatcatata gtgcgttcga
    18421 taatgataga atctttggcg tattagtaca gtgtataaat gatgagtgca acaaatttta
    18481 tagtttacaa tttataaata cgtttaatga agatgaagga ta
//
LOCUS       DAZULG010000057        18148 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000057 DAZULG010000000
VERSION     DAZULG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.058"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..1012)
                     /locus_tag="ACG9CY_001700"
     CDS             complement(65..1012)
                     /locus_tag="ACG9CY_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4938210.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            1406..2698
                     /gene="hisS"
                     /locus_tag="ACG9CY_001701"
     CDS             1406..2698
                     /gene="hisS"
                     /locus_tag="ACG9CY_001701"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS4938211.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            2713..4482
                     /gene="aspS"
                     /locus_tag="ACG9CY_001702"
     CDS             2713..4482
                     /gene="aspS"
                     /locus_tag="ACG9CY_001702"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS4938212.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            4591..5466
                     /locus_tag="ACG9CY_001703"
     CDS             4591..5466
                     /locus_tag="ACG9CY_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4938213.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            5732..7615
                     /locus_tag="ACG9CY_001704"
     CDS             5732..7615
                     /locus_tag="ACG9CY_001704"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4938214.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(7690..7974)
                     /locus_tag="ACG9CY_001705"
     CDS             complement(7690..7974)
                     /locus_tag="ACG9CY_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4938215.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(7964..8698)
                     /locus_tag="ACG9CY_001706"
     CDS             complement(7964..8698)
                     /locus_tag="ACG9CY_001706"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS4938216.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            8827..9450
                     /locus_tag="ACG9CY_001707"
     CDS             8827..9450
                     /locus_tag="ACG9CY_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS4938217.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            9642..10763
                     /locus_tag="ACG9CY_001708"
     CDS             9642..10763
                     /locus_tag="ACG9CY_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4938218.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            10770..13898
                     /locus_tag="ACG9CY_001709"
     CDS             10770..13898
                     /locus_tag="ACG9CY_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4938219.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            13917..17111
                     /locus_tag="ACG9CY_001710"
     CDS             13917..17111
                     /locus_tag="ACG9CY_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS4938220.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(17257..17964)
                     /locus_tag="ACG9CY_001711"
     CDS             complement(17257..17964)
                     /locus_tag="ACG9CY_001711"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4938221.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         6049 a         3228 c         3844 g         5027 t
ORIGIN      
        1 aggaagatca aacagtgctg atcttccttt tttcatatta ctataaattg ataaggtcaa
       61 ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata atcttcattg gataaatgca
      121 aatctaaagc ttggacattg tcagatactt gctctggacg cttagctcca ggtataacta
      181 cagacactcg tggattcttc atgtaccaag ctaaaacaag ttgcgcagga gtggcttggt
      241 atcttttagc aatggggcga agtacatcga cagcggttaa aatttctctg aaacgttctc
      301 cttggaaatc tggatttctt tttcgaggat ctccttcacc aaattgtttt tcctcaccaa
      361 gttcatattt tcctgtcagc aaaccggaag caagaggaaa atagggaaca aaactgattt
      421 tgtttttttc aagataaggg aataattctt tttcagcctg ccgatggatc aaactgtatt
      481 tatcttctac tacatcgaca tatccatctg cattcgcttc tttcaattgt tctaacgtga
      541 agtttgacac accgactgct cggatttttc ctgcttcttt taattcgtgc aatgtagcaa
      601 ctgattcatt ttttggtgtg ctttcatccg gaaagtggat ataaaaaatg tcgatgtaat
      661 cagtttgcag acgttttagt gcatcttcca ccgattgttt gagaaactca ggagaattat
      721 caaatgttcg ccctttattt ggtacatgag cagcttttgt agcgatgacg acacgtgagc
      781 gatcatattc tttttccttc agtacctcgc caatcagttc ttcggaacgg ccgtttccgt
      841 aagcataagc ggtatcgatc aaattgatcc cgctattcaa agctgtacga actacttgtt
      901 ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc tccaacggca tttgctccta
      961 atccaagctg ttctgcgtag acttgtgaat gtccgattct tatttctgcc atagctgatt
     1021 cctccttttt gtctagtcta aaacaaaata ccaaaaagct gaactaaaac gtttttgtga
     1081 ggggaaatag tcagatatct cttgactttg agactggttt ttagtaggtt ataagtaata
     1141 tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt acaagagagg atcatcgagg
     1201 ctgaaaatga tctaaagttt cattagccta aagacactct ggagatggac gagtgaaaag
     1261 ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa atctttcaaa attaggtggt
     1321 accgtgtatg aatttcattc acccttgttc atgatgaaca agggtgattt ttttatttac
     1381 atagctaaga aagggagaga aaactatgag tttccaaaga ccaaaaggaa caaatgatat
     1441 tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa acagcacgtt tattattcag
     1501 agattatcag tataatgaaa tgcgtacacc gatttttgaa cactatgaag tgatatcccg
     1561 aagtgtaggg gatacaacgg atatcgtttc aaaagagatg tatgattttt atgacaaagg
     1621 ggaacgccat gtgacgcttc gaccagaagg tacagcacca atcgttcgtt cttttgttga
     1681 gcataaattg ttcggcccag agtatgcgaa accgttcaaa actttttata tgggaccaat
     1741 gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa ttccatcaaa tcggagtaga
     1801 agcatttgga tcagaaaacc ctgcaactga tgtggagagc atggtgatgg cacttgattt
     1861 cttcaaacag ttggggatca cgcaaatccg gctagtcatc aattcattag gtgataaaga
     1921 aacgcgtcaa gcctatcgtc aagcattgat cgactatctt ttaccttttg aatcagagct
     1981 gagtgaagac tctaaacgcc gccttcatga aaatccatta cgcgtattgg acagtaaaga
     2041 caaacgagat caaaaatttg tggcagaagc tccatctatc ttagattatc taagcgagcc
     2101 tgctcaaaaa cattttgata ccgttgtttc catgctggat gcattggctg ttccttatga
     2161 aatcgacagt aatatggttc gcggactaga ttattacaca catacgattt ttgaaatcat
     2221 gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc gcaggcggac gttatgacaa
     2281 tctagtggaa gaactaggtg gtccttcaac acctggcttc ggatttgcaa tgggtatcga
     2341 gcgattactc ttgactatgg aggcagagga agtagttatc cctgcattta atgagttgga
     2401 tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa gcattgaaag tggtccaagc
     2461 gattcgaaac tttggttttt cagctgaccg cgacttcatg aaccgtaaag caaaagcgca
     2521 atttaagaca gcagataaag cgaatgccaa attagtattg gtgatcggtg aagatgagct
     2581 tgctagtcaa acgattaatg tcaaagcaat ggcaagccga aaagaaaaaa gttttccatt
     2641 agaagagata tatgaacatt ttgatgaagt ctatgatgaa atgactttat tcgagtagga
     2701 ggaaaaaaat aaatggcaca aagaacgatc tattgcggat tagtttccga acaacaatta
     2761 gaacaaacag ttacattaaa aggatgggta caaaaaagac gagaccttgg gggagtaatc
     2821 tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg tctttaatcc tgaaatcaac
     2881 aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat acgtgatcga agtgactggg
     2941 gaagttagaa gacgcggaga aaatgcagta aatcctaaaa tggccactgg tgaattcgaa
     3001 gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa ccccgccatt tcaaattgaa
     3061 gatgatcaag caatcagtga cgaaatacgt ttgaaatatc gttatttgga tctgcgtcgt
     3121 ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa caaaagcgat tcgtcattat
     3181 ttagatgata atgactttct cgatatcgaa acaccttacc tagggaaatc tacaccagaa
     3241 ggcgcaagag actacctagt tccttcacgt gtccatgctg gacatttcta tgcgttgccg
     3301 caatctcctc aattgtttaa acaattgctg atgaatgctg gattcgaccg ttattatcaa
     3361 atcgttcgtt gtttccgcga tgaagattta cgtggagacc gtcagcctga atttacgcaa
     3421 gtagatattg aaacaacgtt cttaacagca gaagaaatcc aaacttacac agagggattg
     3481 attgcgaagg taatgaaaga agttcgcggt atcgaagtca cattgccatt tcctcgtatg
     3541 acctatgatg aagcaatggc tcgctacggc agcgacaaac cggatacacg ttttgatatg
     3601 gaattgatcg atctaagcga tacagtcaaa gaagtagaat tcaaagtctt ccaaatggct
     3661 cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag gcgctgcaga tcgctactca
     3721 cgaaaagaca tggatcagtt aggtcaatat gtaggacaat tcggagctaa ggggcttgca
     3781 tggctgaaag tagaagaaga cggattgaaa ggtccaattg ctaaattcat gggagaagca
     3841 acagaagcaa tcattaaagc aacagatgca aaaccaggtg acttgctgat gtttggcgca
     3901 gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc ggacacgttt aggaaaagaa
     3961 ttaggactaa ttgatgaatc gaaattcaat ttcttatggg taactgattg gccacaattt
     4021 gaattttcag aagaagaagg tcgatatgtc tctgctcacc atccattcac gatgccaaaa
     4081 gaagaagata tcccgttatt ggctgaagac cctgcaaaag tctatgcaga agcttatgat
     4141 atcgtcttga acggttatga actaggcgga ggttctatcc gtattcatac tcgtgagctt
     4201 caagaaaaag tactagaaac tctaggcttt acaaaagaac aaatggaaga acaatttggt
     4261 ttcttgttaa cagctttaga ttacggattc cctccacatg gcggtattgc attaggattg
     4321 gaccgttttg caatgttatt agccggagaa gataatattc gtgaagtcat tgcctttcct
     4381 aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa gtatggtttc gcctttacaa
     4441 ttgttcgaat tgaatattga tgtaacgaat atagaagaat agccgataaa aaggtaaaaa
     4501 ggatttgtcg aatacgcttc ttttgacaac cttgatttaa ttgaaaaaca ctttataatg
     4561 aagccataac atataaagcg gggcttttcc atgaaaaaaa cattcgaaaa ttgggcttat
     4621 catgattaca cgaccaagct ttcggtatct atcgtttatg cgttgatcgc atcagtcgca
     4681 ttgaatttct tttatcagcc aggacatatt tattcaagtg gtattactgg tctagctcag
     4741 attttgacga ctttgtcaaa atcactgata ggattcgaag taccggtatc aacaactttg
     4801 tatttactga atataccttt gtttgtattg gcgtggttaa aaatcgggaa gaagtttaca
     4861 attttcactt ttatcacagt cactttgacc tccctttgta tccatatttt accggaaaca
     4921 gtcctatcta ccgatccaat catctgtgcg ttgtttgggg gggctgtgat gggactaggt
     4981 attgggctag cgttgagaaa cggcctatca tcgggcggtc tagatatcgt gagtatcacg
     5041 attcgtaaaa aaacaggaaa gtcgattggt tctatttcga tctacttcaa tgcattgatc
     5101 gtttttgttg ccggaattct atttggctgg caatatatgt tttacagtac gttgtctatt
     5161 ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac aaaagaagat gcaggtaatg
     5221 atcgtaacga aacatccaga aaaagtagta aatgaaatcc aaaactgtat gcgtagaggg
     5281 atcacgatca tcaatgaagc agaaggtgct tataagcatg ataagcagac agtattgatg
     5341 acgatcgtta cacgatacga actgcctcta cttagagaag caatgaaagc ttctgatcca
     5401 caagcttttg taagtattgc agaaaacgtc cagatacttg gtcgtttcta tgaggatggc
     5461 atttagatcg agtttgacaa aaattcatga aatactgctg tagacagacg ttcgtttgtc
     5521 tgcagttttt ttctaccata gtagaatcag caaagaaaaa ctgctttttt atttgaaatt
     5581 ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt tatgggatga tagaaaaaaa
     5641 tagtagagaa aggacacgat tctacttgct attttcactt aatacccgta tattacgaat
     5701 gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa caaaaaaggt taaaaaacag
     5761 ccgctacatc actgggtttg atgggctccg ttcattagct gtggtgggtg ttatcttcta
     5821 tcaccttctg ccaactagta tgaaaggcgg ctatttgggc gttccgattt ttttcgtcgt
     5881 atctggttat ttgatcacgg atctgctgcg gcaggaatgg gaacaaaatg gaaaaatcaa
     5941 tatttggcaa ttttatgtca gaagaatgaa gagactctat ccggcacttg tttttatgct
     6001 tgtgacagct tcggcttata tcactttgtt tcagcgaggg ctgttaaaca atcttcgagg
     6061 agttgtggtc agcagcttac tgtatgtcaa caactggtgg caaatcaata acggtttatc
     6121 ttatttcgat cgatttgcta atgagtcacc gtttacgcat atctggtctt tagcagtaga
     6181 aggtcaaaac tatttggttt ggccgatctt gtttgtattg ttgatggtat ttgttcgtaa
     6241 aagaaaatgg atcttttata ctgtgttagg tacatccttc ctctccgcca tcttgatgat
     6301 gatcttgtat acaccaggag gcgatccaac ccgtgtttat tacggtaccg acactcgtct
     6361 gttttctata tggttgggaa gtgcgcttgc gtttgtttgg ccaagtacgc gtttaaagaa
     6421 aaacatcccg aaacaagcga aaaatattct gaatatcgct ggaggaatct cgcttttggc
     6481 actgattctt tgcttcttct ttttagatga tcattacaat ttcatctatt acggaggaat
     6541 gctcctagtg agtctgttat gcgtagtgct agttgcagtg acagcacacc ccggagcaag
     6601 tttgaatcga tggctgacaa atccagtatt tacttggatt ggcaaacgaa gctatggcat
     6661 ttatctgtat cagtttccag ttatgatttt ttatgaagca aaggtgactg atattgccga
     6721 tcatgttttg ttgcatactt tagtggaaat cacattgatt ctaatcatca gtgaactttc
     6781 ttatcgatat gtggagcgtc cgctagcacg ttttgattat ggtcgtacat tccaagtgat
     6841 gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa ccatgggttg ttccaggcac
     6901 attacttgta gcaattgcac tcgttggttt cgttacggca ccgaaaaacg ctgtaacagc
     6961 cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa aaattagcag aagaaaccag
     7021 aaacaattca gaaaacacca ctagttcggt tgaccaagcg atccttgata agtacgagtt
     7081 gtccaaggca caagggaata aggcacaaaa attagaacta acggcatttg gtgattcagt
     7141 tatgttggat gctgcaacgg atctacaaga actttatcca aaagtagtgg tagatggaga
     7201 tgtcggacga caattatatg ccagtgtacc gtacattgaa aaattaaagg aacaagattt
     7261 gttgaaagac actgttctga tcggactagg aacgaatggc tcattctcgg aagctcagtt
     7321 tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac tgggtcaatg tccgagtacc
     7381 gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa cagatgacga aaaaatacga
     7441 caatatgacg ctgattgatt ggtacagcta cagtaatgac catagtgact ggttttatga
     7501 tgatcaagtc catccaaatc ctgaagggat gacacattac attcacttag tcagcgaagc
     7561 cttgctagga ggaagttcaa gtacgaacag cagtgagaca aacgtgacag aatgatagct
     7621 acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag tttttttata tttaccgtgt
     7681 ttttaacatt catatagatt caggaagcaa aacggttgag tgtgtctgta aataacgttc
     7741 tttttgtttt cgggtgagtt ttttgaatgc agcttctgct tttgttgctt cgcttcttga
     7801 agaaaatact tctgcatgga tcatcttaac aggtcttctt ttttgaagac gagtatattt
     7861 agcaccagta ccattgttat gttcctctaa tcgtctagaa agatcggttg tatatccacc
     7921 ataaaatgtc tgatctttac agaataatac gtaaaaataa tgttcatttg ccatagagca
     7981 tctcacgaat ctctgcagtg tattctccat ccatattgta agtgatcaat ggtggagcga
     8041 ctttgaatcc gtctaatttg ccttctttga ttccttcaac taacaaaata tttgcctctt
     8101 ttccaggttt tgggtaaaca aattggatcc ttttaggtgc aattcgatag tcttccatga
     8161 cctgaatgat ttctaagaac cgatcaggac gatggaccat agtaaagcgt ccatttgttt
     8221 ttagtagctt acttgaaata tcgatcactt cttctaacgt cgtatgaatc tcatgacggg
     8281 cgattgccag gtgtttatta gggttcgttt tatttgttgg cagccctttg aaataaggtg
     8341 gattacagac gactaagtca caagaattat gtcgaacgac agataagctg tctttcagat
     8401 cgatggtgtg gacagtcatt tgttcattta aatggttaag cgcgatacta cgtcttgcca
     8461 tgtctgctag acgaggttga agttcgatag cgtcgatagc cgcctgggtt cgtttactta
     8521 aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac aatcttccct tttttaggta
     8581 cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa agaaaacact ttggaacttt
     8641 ggataatctt gatatcttct gcgtataatt ggtcgattcg ttcgtcttcg tgaagcatgg
     8701 attccctcca aaatgaaaaa aatagtcgtc tgtattatac tatttttatt aaaaagaacc
     8761 aaggtggctt gtcaaacctc tattttttat gcatactatg accataagaa gggaaaggaa
     8821 gagactatgt tttatcgatt tatgcgcggg gtagttcgag tactattagc cttagttaat
     8881 ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag atgagaatta catactagtt
     8941 gctccccatc gtacttggtg ggacccatta tatctggcgg tagctgcagc acccaaggaa
     9001 ttcagcttta tggcaaaaga agaactgttc aaaaatccag ttctgcgatt tatattaact
     9061 cgatccaatg cttttccagt taaaagagaa aatccaggac caagcgtcat taaaacacct
     9121 gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt ttcccagtgg gacaagacat
     9181 gctacagaac ttaaaggcgg aatggcattg atttccagaa tgtctaaagc gaaaatcgtg
     9241 ccagctgtat atcaaggtcc gttgacactt ggggatctgt tcaagcgtca gagagtgaca
     9301 gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga aaacggataa agaaggtatg
     9361 gcggagatcg aacgacgaac acaagcagct tttgacaaat tggacaaaga agtgaaccct
     9421 gatttcaaat atgaagtcaa aaaaaaataa aatgataagt ttatgcgtgg gcggtctttt
     9481 gaatctactg tcctgttcag ttcagacttt ttcaatgaaa acgagggaga gacgtcaagt
     9541 ctgccctctt tttgcgttcc aatatgtaaa atagaagaca ggtgtcatgt aatggatcaa
     9601 agtgttataa taattagaag gacatttttt gaggtgattg aatgcgtttt ttacatacag
     9661 ccgattggca tatcggaaaa aaactacaag gttatgactt gattgaagac cagcgtcatg
     9721 tactatcttc tatactagat atcgcgaaag aagaaaaagt agaggcaatc gtgattgccg
     9781 gagacttata tgataggagt gtcccagctg ttgatgccgt agaattatac aatgaattga
     9841 tggtcgattg gaatttaaag gaaaagatcc cgatttttgc tatatcagga aatcacgata
     9901 gcagtacgag actttcagcg ggtacgccat ggttcagtta ttcgaactat tttttgcgaa
     9961 cacagctagc agaagcattt gaaccagtcg agtttaaaaa cgtacaattt ttctttcttc
    10021 cttactttga accaattgca gcgagattat attttgaaga tgacaatatc cgaacgatca
    10081 agcaagcggt agccaaggtg atagataaaa tgcaggagct atttgattca gaaaaaaaac
    10141 aagtattgat tagccatttt tttgtaatgg gaagtgtaaa atctgattcg gaaacgaaaa
    10201 tcgaagtagg cgggttggat tcgatccctg gtgaactact tacaccgttt gattatgtag
    10261 cactaggaca tttgcataat caagctgctt tgagacagga taatgctcgc tatagcggtt
    10321 ccccattgaa attctcttta tctgagatca accaaaaaaa aggcgtatgg atcgttgacc
    10381 tgacaacagg cctggagttg aattttaggg agatcgaacc cctgcatgat attgaagaaa
    10441 taacagcgag tttcaaagaa ttgctggatc ctgcctttta tcagtccgtc aatcgggaga
    10501 attacatcta tatctatctc actgatagaa cgatcatccc gaatatgatg aaccagcttc
    10561 ggcaaatata tcctcgtatt ttggggattg aacgggcaaa cggtcgagca gaacaaacaa
    10621 agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa attagcagaa gcatttttta
    10681 gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa atggctcgga gaaacgttgc
    10741 aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa aaaattacaa cttaaaaatt
    10801 ttggtccttt tatcaatgag accgtagatt tttcacgttt gacagaagca ccgctttttt
    10861 tgatcagcgg gaagacagga gctggcaaaa cgacgatttt tgatgggatc accttcgcac
    10921 tttttggtga aacttccgga agacttcgct ctggaaaaga gatgcgttct ttgtttgcta
    10981 caccagacga agaaacaagc gtgacttttt cttttgaaca tcagcagatg agctatgaaa
    11041 tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg aaacggaaca aaaaaacagg
    11101 cagccaaagt aacgttgacc atttttgatg ggaaaggaaa agaaataaaa caaatcacaa
    11161 aacgtacaga agcagatcag ttgatcagag aactgatgaa tctcgatgcg aaacagtttt
    11221 ctcaaatcgt tttactaccc caaggagaat tccgtaattt tttggtttca tcaagcagtg
    11281 ataaagagac tgttttacgt catttattcg gtactcaatt tttccaacag ttcaatgagc
    11341 gattaaaaga aaaagcaaaa caacagcaac aaaaattgga tcatttagag caagagctgc
    11401 atcttctgca aaaaaggttt gttttagtca aagaagagga accagcagca gggtttgaag
    11461 ccgttttagc ccaatggaca aaccatcagg caatactcga aaaacaaatc acggaagaaa
    11521 aagaagctct aacggaactt caggaaaaac aaaagcaact agaaaattcc tactatcgtt
    11581 ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt aataaacaag caaaaggaat
    11641 tagctgctca aaaagacgaa atagaggaaa agaaacgatg gattgggtat tatgaattta
    11701 cgcaacgatt agtcgaacca atccagcgcc tacaagaaat aaaagttgaa aaaatagaac
    11761 tgtcacaaaa aagtagtgag caaacaaaga aactagcgat gactacgcag aattatgaag
    11821 agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac aaaaatcaaa gaagctgctc
    11881 aaaaactgca aaacaaacag atgatggtgc caattgtcga atccttacgt agtaaaaaac
    11941 aagacgcaga acaattaaca atttcattgg ataaagaaga attgaatgcc gagaagctgt
    12001 atcaaaatct cgctatctat caggaaagaa ctcgaattct tcaagaaaat ttactggatc
    12061 aggaaaaact gcaagaagat cgtcttgctt ttggacagtt aaaatatcta gatgaaaagc
    12121 agcaagaaat agaaaaacaa aaagcaaaag acactgggtc gctcgcacag ataagagaag
    12181 aactcaaaaa agcaactgaa gaaataacaa aaaatagtca gctattgaaa gaacagacag
    12241 aacaagcaga acagctgaaa agccaatggg caaagcagca aattgctcgt cttcagcttt
    12301 ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac ggaacacccc atacaacaat
    12361 taaaacacga acagccaaat aaggaagata tcttaaaagt agaacaacaa ctggcagaag
    12421 ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa aagtgaagca catgaaaata
    12481 atcttcaaca acaattatca gatttagaac aaaaaatcat ggcaacgaat atacaattgg
    12541 ctgaacaaga agaacacttg caacaggctt tgaaaaaggc atttcctgag ataacagaac
    12601 aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt agatagacga aaaagcaaga
    12661 tggccaatgc aagaatagag ctggaagaaa taagacaaca agcagaaaag ctcgaacaac
    12721 aattagctgt ctacaaacag cagcagacag aaaaaaataa tcagctccag caactgcaag
    12781 gagaaatcaa aagtttagaa caacaattgc aagatacgga tagtaactgt ttagaagagg
    12841 agatacgacg acttgaaaaa cagttagaag aagactcgaa tcaactagct gttgagcaga
    12901 aagcaggagt gcaattgttg caagatcttc atacgcttcg gacacagctt gatcttcaac
    12961 atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga acaagaaaga atagctaaaa
    13021 agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat ctgtgcattt gcacaacagc
    13081 aagcacaata taaagaaaac caacgtacag ttacttctta tcatgaagag caattagtga
    13141 cagctgaccg gctgcgtcaa ttagaaaata tccagctaac tgagacattc ccagatacag
    13201 aagagatcag ccaacagtta gaagaaataa gacgtatgat cgaaataaag caagaaaaga
    13261 tatacggttt acaagaacaa aaaattagta atcaaaaaat ctacgatgaa tgtctttcga
    13321 tttatcacac aagccagacg aaattagatg agcttacgca attacagcag ctttcgcaaa
    13381 cattcaacgg agaaaatcct aagaagacta gtttggaacg gtatgtgctt caggtttatc
    13441 ttcaagaagt gttacaagta gcaaatgatc atctccaacg tttaacgaaa aatcgttatc
    13501 aatttgagct agcggatacc attggcagtt atcgagggaa aaccggatta gaaatcaata
    13561 tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac cctgtctggc ggtgagagtt
    13621 ttattgcagc actttcttta gcattagctc ttgccgatgt gatccagaca caagctggtg
    13681 gtgtagcaat cgatgcattg tttattgatg aaggatttgg gtcattagat gaagaagctt
    13741 tggaaatggc aatggaagct ttagaaacga tcgaaagtga aggaagaatg atcggcatca
    13801 ttagtcatgt aagagaattg aaagaacgag tattacaaca aataagaatt gatacagaag
    13861 gtagcggaca aagcaaaata cgctatcaac ttggataaaa agggagattg gtaactatga
    13921 aatggagtat tccagaaaaa gtcattgcac gtggacgtca atacttgcaa gaaggccgag
    13981 tgttatcggt tgtccctgac caagaagaaa acgtgtggca tgcagaagtc ttgggcagtg
    14041 aattgtatat ggttgatttg gatggtacag caaaagagat agattactgc caatgtccct
    14101 attgggagga acataaatat tgtaagcata cagttgctgt ggaactttat ttacggtcga
    14161 aaaatctatc tcgtatcatg aaaaaagacc agccaatgct agaaccggta aaagcaacaa
    14221 gtgagggaga aatcattgca aaaggttttt ctcgtctaaa aacacaaaac aatcctcaag
    14281 cggtgcaacc tttgattatt gaataccaag tagaaacgat tgagactaat cagtatcacc
    14341 cagagctttc tattttggca ctttacttgc gtatcggttc attggcatca ccgaaaaaaa
    14401 catatatcgt aaaaaacatc tatgattttt tacaagctta cacagaaaaa gaaacttaca
    14461 ctgttaataa acagtaccag tttcgtctag ataaacgggc gtttcaaaaa gaagatcaaa
    14521 aaatactcac gcatcttgca ggaagtgctc agacgcatca attattaggt acgaacggct
    14581 tacaagtcaa aggaaaacta gataaaagat atctgcttct cccagtagaa caagcccgct
    14641 tcctgttaga gcagatgaat cagactacac gtttgcatct gcagataaat gaacaaaacg
    14701 atcaaggaat acaattttcc gatcaaaaca aacctttatc attcaaagtg gaaaaatttg
    14761 gagaagatta tttgctgcaa gcattgaatg attttgattt tttctttatg cattatcagt
    14821 ggggagttat ccaagggaca ttttattctc tgacgaaata tcaacaaacg gtttatcaaa
    14881 cttggaaaca attgattcgc cgattggaga aaccagaagt agtgttcaag aaaaaagaat
    14941 taccgtcttt gtttaaagag attatccctc tgctgtcaga gatcggcgac gtagctattg
    15001 cccctgcagt agaagaggaa gtagcagata tccccgtcga atttattttc tttttccgaa
    15061 aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta tggcgaagtc atctattcga
    15121 ctgacccgaa gcatgaggtc tcgacagaac aggcttttca ggtgttacgt gacacagtag
    15181 aagagaaacg agtacttgat ctattcagaa tgtatcgcta ccagaaaaat gaaactggtt
    15241 atgaacgtcc attgccaagc ggggaagaac tatatgcctt tttccgcaca gaactatctg
    15301 tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt gcgagagttg tatttagatg
    15361 cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg ttcatggctc gatattcgat
    15421 tcgatgtgac agggatagag gagcaagaaa tcgatcatgt cctgcaaagc ttgctgagaa
    15481 atgatgcttt ttatacatta gaaaatggtc aggttctttc ttttgattca gaagaatttc
    15541 aacaaacaag ccaagtgttg caacaattac gggaatcgat acgtacagaa gaaggaacga
    15601 tccatgtacc taaaaatcaa ggattgatca ttcaaaatca attggaaaaa agcaatgcta
    15661 cttttagtga atcgttccaa acgatggtac aagatctcat tcatccagaa agataccagg
    15721 cacagcttcc caaaggattg aatgcaacga tgagagatta tcaaaagcaa ggttttcgat
    15781 ggttgaagat gttaggtcat tatcagtttg gcggtatttt agccgatgaa atgggacttg
    15841 ggaaaacact acagacaatt gcctttttac tttcggaaaa agaagaacgg aaaagcttct
    15901 ctgctttgat cgtagcgcct gcgagcttaa tttacaactg gcaggcagag gtgagaaaat
    15961 tcgccccttc tttaagtatc caagtaatca atgggaataa aaaagaacgt gaagaattac
    16021 tggctaaaga cacagatatc cgagtgactt cttatgccag cttaagacag gatctagcta
    16081 attatcagtc gcaaaaaatt gattatttga ttcttgatga ggcacaaatg gtcaaaaata
    16141 gcagtacgaa aactgcacaa gcattgagag aattagctgt acctcagcgg tttgcactga
    16201 gtgggacacc aatcgagaat aatttagaag aattgtggtc attgtttgca acgattatgc
    16261 caggattttt cccgactaaa acaaaattca gggaacttgc cgcagaagaa attgcgcaaa
    16321 tgattcgacc gtttatttta cggcgagata aacaaactgt gttgaaagac ttgccggaaa
    16381 aaacagagat gaatctatat tctgcactta cagaagaaca aaaaactgtt tatttagctt
    16441 acttgcgtca aatgcgtgaa gaaatcacat caatggattc tgaagcattc aagaaaaacc
    16501 ggatcgggat acttgctggt ctgacaagac tgcgacaaat ttgctgcgat cctcgtctgt
    16561 ttattgaaga ttaccaaggc ggttccggca agctcgaaca agtaaaagat ctactggtag
    16621 ctgccaaaga gaacaaacga agagttttgc tgttttctca atttacaggg atgctgacta
    16681 tcttgcaaga agagttagcc gagctaggga tctcgacatt ttatttaaga ggcagtacga
    16741 aaccacaaga tcggttaagt atggtagacg catttaatgc tggcgaaaaa gatgtttttc
    16801 tgatttcctt aaaggctggg ggaactggat tgaatctcac gggagcagat acggtcatct
    16861 tgtatgacct ttggtggaat cctgccattg aagaacaagc tgctggacgt gcacatcgga
    16921 tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc tgaaggaacg attgaggaac
    16981 gcatggattc actccagcaa gaaaaacgcg aactgttcca aaaagtgatc caaggtaatg
    17041 aagaacagtt gacgaaactg actgaagaag acatccgcat gattttaagt gtaggagaaa
    17101 tcgaggagta agagactcga aaaagcgttc agtaatgggt aagaaagcct ttctaaagaa
    17161 gcgattgttt atttgattta aaaaatagca aggaggctgg gattccattt tatcccagct
    17221 tccttgctat cattttttta tttctatttt taaaggttat acaaagattg tcttcattgt
    17281 ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa attggagtct gttcaatttt
    17341 gcgttctcga tggttgaacc aaagcgcttg ccagttagca ctttttgcac ctagaacatc
    17401 attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga agcatatgaa attctttctc
    17461 agctagctga aaaatctctt tttctggttt ttggaaacct gttgcttgag aaatgagcat
    17521 attgcgggaa ggaatccaat tgttcaattg aagttgcttc agtttttttg tctgatggtc
    17581 agtcggccca tttgtgataa ttcctaatgg gacattcttt tccttcaaga aatcgagggt
    17641 ttttttgacc tcctcatgca agcagatatt atcaagttct tcctcgtaaa ttttttgaaa
    17701 taagaggcca tcttcttccg taatatgagg gtagtctaaa tcttttagtg attggctgat
    17761 ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc attacttttg ggaagttttc
    17821 atcactatga tggcggaacc ggatatatag tggatgcata tctgcatctg aaacaagcgg
    17881 aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga tcatacatcg tatcatcaac
    17941 atcaaacaca actgatttaa tcaaaatttt acacgccttt cggttttcat tgattttttc
    18001 acaaatcctt gactattgta acagaaaaaa ttgtctaaaa aagctgataa ataaaggttt
    18061 ttcttactat atttctattt ccttgacaga ataaggtttt tatatttcag tttttttcac
    18121 taaaaatcgt gaaacacgtc aataagtt
//
LOCUS       DAZULG010000058        17906 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000058 DAZULG010000000
VERSION     DAZULG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.059"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..636
                     /locus_tag="ACG9CY_001712"
     CDS             133..636
                     /locus_tag="ACG9CY_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4938222.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="ACG9CY_001713"
     CDS             763..1800
                     /locus_tag="ACG9CY_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFS4938223.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="ACG9CY_001714"
     CDS             1815..3512
                     /locus_tag="ACG9CY_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFS4938224.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="ACG9CY_001715"
     CDS             3505..3966
                     /locus_tag="ACG9CY_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFS4938225.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="ACG9CY_001716"
     CDS             3979..4839
                     /locus_tag="ACG9CY_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938226.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="ACG9CY_001717"
     CDS             4877..5266
                     /locus_tag="ACG9CY_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4938227.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5253..7700
                     /locus_tag="ACG9CY_001718"
     CDS             5253..7700
                     /locus_tag="ACG9CY_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4938228.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="ACG9CY_001719"
     CDS             7705..9813
                     /locus_tag="ACG9CY_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4938229.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="ACG9CY_001720"
     CDS             9810..10814
                     /locus_tag="ACG9CY_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4938230.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="ACG9CY_001721"
     CDS             10833..11744
                     /locus_tag="ACG9CY_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4938231.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="ACG9CY_001722"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="ACG9CY_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12406..12621)
                     /locus_tag="ACG9CY_001723"
     CDS             complement(12406..12621)
                     /locus_tag="ACG9CY_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938232.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13089)
                     /locus_tag="ACG9CY_001724"
                     /pseudo
     CDS             complement(12880..13089)
                     /locus_tag="ACG9CY_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(13392..14831)
                     /locus_tag="ACG9CY_001725"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="ACG9CY_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="ACG9CY_001726"
     CDS             complement(15379..16050)
                     /locus_tag="ACG9CY_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS4938233.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="ACG9CY_001727"
     CDS             complement(16399..17850)
                     /locus_tag="ACG9CY_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4938234.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT         5832 a         3012 c         3466 g         5596 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgatttacc tctctttttt ctctttaata
    17881 gtgttttcta ttaaaaatta tgataa
//
LOCUS       DAZULG010000059        17152 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000059 DAZULG010000000
VERSION     DAZULG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.060"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG9CY_001728"
     CDS             complement(24..722)
                     /locus_tag="ACG9CY_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS4938235.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG9CY_001729"
     CDS             complement(715..2067)
                     /locus_tag="ACG9CY_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS4938236.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG9CY_001730"
     CDS             complement(2290..3690)
                     /locus_tag="ACG9CY_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4938237.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG9CY_001731"
     CDS             3851..4588
                     /locus_tag="ACG9CY_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS4938238.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG9CY_001732"
     CDS             4713..5192
                     /locus_tag="ACG9CY_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS4938239.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG9CY_001733"
     CDS             5189..5956
                     /locus_tag="ACG9CY_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS4938240.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG9CY_001734"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG9CY_001734"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4938241.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG9CY_001735"
     CDS             complement(6859..7461)
                     /locus_tag="ACG9CY_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4938242.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG9CY_001736"
     CDS             7567..7797
                     /locus_tag="ACG9CY_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938243.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG9CY_001737"
     CDS             7788..8036
                     /locus_tag="ACG9CY_001737"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4938244.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG9CY_001738"
     CDS             8142..8726
                     /locus_tag="ACG9CY_001738"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4938245.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG9CY_001739"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG9CY_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS4938246.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG9CY_001740"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG9CY_001740"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS4938247.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG9CY_001741"
     CDS             10627..10806
                     /locus_tag="ACG9CY_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938248.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG9CY_001742"
     CDS             10828..11853
                     /locus_tag="ACG9CY_001742"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS4938249.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12044..13288
                     /gene="glyA"
                     /locus_tag="ACG9CY_001743"
     CDS             12044..13288
                     /gene="glyA"
                     /locus_tag="ACG9CY_001743"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS4938250.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13441..14388
                     /locus_tag="ACG9CY_001744"
     CDS             13441..14388
                     /locus_tag="ACG9CY_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS4938251.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14522..15151
                     /gene="upp"
                     /locus_tag="ACG9CY_001745"
     CDS             14522..15151
                     /gene="upp"
                     /locus_tag="ACG9CY_001745"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS4938252.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15290..15532)
                     /locus_tag="ACG9CY_001746"
     CDS             complement(15290..15532)
                     /locus_tag="ACG9CY_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938253.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15653..15725
                     /locus_tag="ACG9CY_001747"
     tRNA            15653..15725
                     /locus_tag="ACG9CY_001747"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15686..15688,aa:Thr,seq:cgt)
     gene            complement(15844..16482)
                     /locus_tag="ACG9CY_001748"
     CDS             complement(15844..16482)
                     /locus_tag="ACG9CY_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS4938254.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16605..>17152)
                     /locus_tag="ACG9CY_001749"
     CDS             complement(16605..>17152)
                     /locus_tag="ACG9CY_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4938255.1"
                     /translation="ILSQKEVIENQIICHPIIYKSIFKYAFFASNDFQRMEITDCIFE
                     NCDFSGCILTKSMLHRVKFVNCKLTGTKLMESYIGNTLFENCKMDYVNLSGSNIKEST
                     FEHSVLSSADFVDCSLTKIMFSTNDLENASFFDTDLKNIDLSSNSFEMIEVRSEHLKN
                     CTLNQLQALGFARKFLQIKVV"
BASE COUNT         5851 a         3040 c         3415 g         4846 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaattgtagg aaaataaaaa ggagctgttg tttgtggtag attacaaaac
    12061 gtttgaccct gatctttggg ctgcgatcgc caaagaagaa gaaagacaag aacataattt
    12121 ggagctgatt gcctctgaaa actttgtctc cgaagcagtg atggctgcac aaggaagtat
    12181 cttgacaaat aaatacgcag aaggttatcc agggcatcgc tattatggtg ggtgtgaatt
    12241 tgtcgatatc gttgaaaatt tagcgattga ccgtgcaaaa gaattattcg gagcgaaatt
    12301 tgcgaacgtc cagcctcact ccggctctca agccaacacg gctgcttatc tcgctttggt
    12361 agaaccaggg gacacgatct taggaatgga tctttccgca ggcggacatc taacacatgg
    12421 atcaccagtc aacttcagtg gcaaaaccta tcactttgtt gcttacggtg ttgatccgac
    12481 aacagaagtt attgattata atgtggtacg tattttagct agaaaacatc aaccaaaatt
    12541 gatcgtagca ggtgccagtg cttacggacg aacgatcgac tttgcgaaat tcagagaaat
    12601 cgcagatgaa gtaggcgcaa aactgatggt agatatggca catatcgcag gattagtagc
    12661 agcaggactt catcctaatc cagttcctta tgcagatatc acaacgacta ccactcataa
    12721 aacattgcgt ggccctcgag gcggcatgat tttgacaaac gatgaagcat tagcaaaaaa
    12781 aataaatagt gcggtatttc ctggtattca aggcggccct ctagagcatg tgattgcggg
    12841 taaagcagta gcattcaaag aagcattaga tcctgcattt aaagaatata gtgaacaaat
    12901 catcgccaat gcaaaagcaa tggtcaaagt tttcaatcaa gcaattggaa ccagagtcat
    12961 ttcaggggcg acagataatc atctgatgtt gatcgatgtt cgcgagttag gtataaatgg
    13021 aaaagaagca gagagcattt tggacagtgt gaatatcact gtaaataaaa actcgattcc
    13081 ttttgaaaca ctaagtccat tcaaaacaag cggtattcga atcggcacgc cagcaatcac
    13141 tactcgcggg ttcaaagaag aagatgctgt aaaagtggct gaactagttg tcaaagcatt
    13201 acaagcaaaa gatgataacg ctcaactaga cgaagtcaaa acaggtgtac gcgaattaac
    13261 tgaaaaattt ccgttgcata aaaaataaaa atttaaattc cattagtaaa cagaaaagtt
    13321 caatgttggg cttttctgtt tattttttgg gaaaacggat tctttgacaa tatcggtgat
    13381 gctctctagt atatgaggtg taattttagt cttcacacta aatctcaggg aggaaaagtc
    13441 atgactgatc gtatctcgtt agaaaaggaa gcagtagcat tcagtgatgc aaatgcgcct
    13501 catccgcgta tctatgaact agcaccggaa gaaggaagag ttttactaga agaagtacaa
    13561 gaatcacctg tgaacaaata ttccgtcgat attgaagact tgatggtgga tactggcgaa
    13621 tggggaaaac tcaatattcg tctcgtacga ccagaaggga atatggataa attaccagtt
    13681 attttttata ttcatggagc aggctgggtt tttggcagtg ctcaaaccca tgataaattg
    13741 atcagagaac ttgcggttcg tacgaattcc gtcgttgtct ttccagaata tagtcgatca
    13801 ccagaagcaa aatatccaac agcaattgaa caaaattatg ctgtgttgca acagctgaat
    13861 aaattagcca agtcaaaaaa tctggatctc gagaatctaa tcgttgcagg agattcagta
    13921 ggtggtaaca tggctacagt catgacgatc ttaacaaaag aacgaaaggg cctcccaata
    13981 tccaagcaat tgctgtatta tccagtcaca aacgctgatt ttgatacaga ttcctatcat
    14041 gaattcggag aaaactattt tttgacaaga gaaggaatga tgtggttttg ggaccaatat
    14101 acgacgaatg aagaagagcg tgctgaaatc acagcttcgc ctttgagagc aaatataaat
    14161 gaactaaatg atcttccttc agcaatgatt ttaacgggag aagcggatgt attaagagat
    14221 gaaggtgagg cttatgcgcg caaacttcgt gatgcagggg ttcctgttac acaagtacgc
    14281 ttccaaggaa tcatccatga ttttgtcatg gtaaatgcta tggatcagac aaaggcaacg
    14341 cgtgcagcaa tggcattatc tacttcttgg atcaagaaaa aagaataagc tttgcttgtc
    14401 cgcaggaaaa cttcttttca aaaacggatt ttcttaggaa aaatggcgag tttgactagt
    14461 caatcacttg attttgtttt acaattaaaa agaaacgaca gaacaaagga gagcttaaaa
    14521 aatgggcaaa tttcaagtga tcgatcatcc attgattcaa cacaaactaa ctattattcg
    14581 ggacaaaaac tgcggaacaa aggtttttcg tgaagtagtc gatgaaatcg ctatgttgat
    14641 ggcatatgaa gtatcacggg atatgccatt ggaagacatc gtgatcgaaa caccgatcac
    14701 tgaaacaaca caaaaaacgt tgtctggtaa aaaagtagcg attattccaa tcttgcgcgc
    14761 aggtattgga atggttgacg gtatcttgga attgattcca gctgctaaag tgggacatat
    14821 cggtctttat cgagatgaag aaacattgga accacatgaa tatttcgtca aacttccaga
    14881 agacattgat gcacgtcagt tgtttgtagt agatccaatg ttggcaacag gcggatcagc
    14941 aatcatggca atcgatgcat tgaaagaacg tggcgcgtcg aacattaaat ttgtttgttt
    15001 ggtagcagca cctgagggag taaaagcttt gcaagaagcg catcctgata ttgacatcta
    15061 tacagcttct ctagatgaaa aattgaatga acatggttat atcgttcctg gactagggga
    15121 tgcgggtgac cgtttgtttg gtacaaaata agtaaatacg aaaaagggtg tgacacaact
    15181 tttgtcacat ccttttttgg tcatttttaa cgtttgatgc agaacagctt tttcacagct
    15241 ttcgtttgca tataaaaaaa ccatcccaaa aatgcagaga tggtcaaatt caaatctttt
    15301 ttagttcttt gtagttaacg attactacac ccttactttg cgtcatcaag ttcgcatttt
    15361 ctgcagtttc atcaatctca acaacagcgg aatttttcaa ttgctttgaa atcgttccag
    15421 tttgcgactt gccgtgaatt gtgaacatta cgtgagtatt gatcttgaat ttttcaccaa
    15481 tttccgatgg tacgatagtt gtatcaaatt ttccaaaagt tgccattttc ataaaacctg
    15541 cctttcacat caattatacc acgttttatg aaaaatatca tgaaattaaa ctaattttcc
    15601 atcggactct ttacaatcag ccaaatagag tatataattt cttttggata aggccagctt
    15661 agctcagttg gtagagcaac gcactcgtaa cgcgtaggtc gtaggttcga tccctatagc
    15721 tggcattaaa agagaagaca gatatatcaa ggatttttaa tccattgata catctgtctt
    15781 tttgtttaaa ggaaataaag tatgctttct tagcaataga gcatgtatta tttctataaa
    15841 aatttaggag aataaaacga ctagcaatat agctgtgaaa tcaaatatca catgaactgc
    15901 ccaacttgta atgatcgagt tgcttttaat ggcagcttgg ttgaataaca atcttgccac
    15961 cccttgtatc aaaaggatgt gtgctacagt gtgtaaaata tttccattat cataagtact
    16021 gtaatggata aatccaaaga tgattgctga caagaaacta gcgacaggaa tgctgaactt
    16081 actagaaaaa atagcgagaa aatacactga gaacaattct tctccaagta tcatgatagg
    16141 caaaataata aataataacg ggctgaaagc ctcatttatt ggattgcctg ttaaacccca
    16201 tttcaaaatt tgttgcaaaa ataccgagac tgcggcactg attactacat tgataaaaaa
    16261 atatttggct atatttttga caggagcgac aggtttcgag aacatttttt tcgtatcttt
    16321 tttaccaaaa acaagtgtaa atgctataaa tccagtgata gttaaagaaa aaatgctaat
    16381 aatggatggc gcaaatagca accctaacaa accgagtaca acgacaatag tgcccaatat
    16441 ccctttttta agttctattt tttttcctaa aatgttattc atgataataa ctcctactat
    16501 tcagtttttg aagatagagt cagtatacat gaagagttaa aataggcaat gtttttttat
    16561 ggatatttgt attgctaaaa atttatctta tatattcatt ttgattatac tacttttatt
    16621 tgtaaaaatt ttctagcaaa accaagtgct tgcaactgat taagcgtaca attttttaga
    16681 tgttccgagc gtacttcaat catttcaaag gagttgctac ttaaatcgat attttttaaa
    16741 tcagtatcaa aaaagctagc attttctaaa tcattagtag aaaacattat tttagtgagg
    16801 gaacagtcta caaaatctgc gcttgataag acagagtgct caaaggtact ttcttttatg
    16861 tttgaaccag ataaatttac atagtccatt ttacaattct cgaataacgt gttcccaata
    16921 taactttcca tcaatttagt acctgttaat ttacaattga caaatttcac gcgatgcaac
    16981 atgctttttg tcaatataca gcctgaaaaa tcacagtttt cgaatataca gtcggtaatc
    17041 tccattcttt gaaaatcatt ggaagcaaaa aatgcatact tgaaaataga tttgtaaata
    17101 attggatggc agattatttg gttttcgatg acctcttttt gtgaaaggat at
//
LOCUS       DAZULG010000060        16961 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000060 DAZULG010000000
VERSION     DAZULG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.061"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(284..2176)
                     /locus_tag="ACG9CY_001750"
     CDS             complement(284..2176)
                     /locus_tag="ACG9CY_001750"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938256.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2173..3900)
                     /locus_tag="ACG9CY_001751"
     CDS             complement(2173..3900)
                     /locus_tag="ACG9CY_001751"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938257.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3916..4566)
                     /locus_tag="ACG9CY_001752"
     CDS             complement(3916..4566)
                     /locus_tag="ACG9CY_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS4938258.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4770..5066
                     /locus_tag="ACG9CY_001753"
     CDS             4770..5066
                     /locus_tag="ACG9CY_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4938259.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5238..5858
                     /locus_tag="ACG9CY_001754"
     CDS             5238..5858
                     /locus_tag="ACG9CY_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4938260.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5950..7116)
                     /locus_tag="ACG9CY_001755"
     CDS             complement(5950..7116)
                     /locus_tag="ACG9CY_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4938261.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7128..7481)
                     /locus_tag="ACG9CY_001756"
     CDS             complement(7128..7481)
                     /locus_tag="ACG9CY_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4938262.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7609..8631
                     /locus_tag="ACG9CY_001757"
     CDS             7609..8631
                     /locus_tag="ACG9CY_001757"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS4938263.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8650..8811
                     /locus_tag="ACG9CY_001758"
     CDS             8650..8811
                     /locus_tag="ACG9CY_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938264.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9070..9504)
                     /locus_tag="ACG9CY_001759"
     CDS             complement(9070..9504)
                     /locus_tag="ACG9CY_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938265.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9614..11473)
                     /locus_tag="ACG9CY_001760"
     CDS             complement(9614..11473)
                     /locus_tag="ACG9CY_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4938266.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11474..11614
                     /locus_tag="ACG9CY_001761"
     CDS             11474..11614
                     /locus_tag="ACG9CY_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS4938267.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11591..12751)
                     /locus_tag="ACG9CY_001762"
     CDS             complement(11591..12751)
                     /locus_tag="ACG9CY_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4938268.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13058..13969)
                     /locus_tag="ACG9CY_001763"
                     /pseudo
     CDS             complement(13058..13969)
                     /locus_tag="ACG9CY_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(14135..15328)
                     /locus_tag="ACG9CY_001764"
     CDS             complement(14135..15328)
                     /locus_tag="ACG9CY_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4938269.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(15412..15612)
                     /locus_tag="ACG9CY_001765"
     CDS             complement(15412..15612)
                     /locus_tag="ACG9CY_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4938270.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(16036..16269)
                     /locus_tag="ACG9CY_001766"
     CDS             complement(16036..16269)
                     /locus_tag="ACG9CY_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938271.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(16276..16707)
                     /locus_tag="ACG9CY_001767"
     CDS             complement(16276..16707)
                     /locus_tag="ACG9CY_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4938272.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         4770 a         3705 c         3182 g         5304 t
ORIGIN      
        1 aagtaataag aacagccaaa caaaaatagc tcctcctatt tttcgtttgg ttgggcttat
       61 taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtaaaat cagcggtttg
      121 acctgagtat tagagataca aaacggaaaa atagcttctc atattttgcc gttttttgag
      181 ctaataccga aggacagctg attgaacacc gtttattcgg aaaaagggac tgtgacacga
      241 cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa gcttcttctt
      301 cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc aatgtatcgt
      361 ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct gcatcgcgta
      421 ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg cggttcatcg
      481 ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca tcaaggatca
      541 ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc tgcccttgcg
      601 aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat ttacgaacaa
      661 aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt
      721 tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct tgcagtacca
      781 ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac
      841 gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc
      901 ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc aagttaaaat
      961 cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt
     1021 atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct tcttcatcca
     1081 acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc atcaaacttg
     1141 caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct tgaacatccc
     1201 ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg aacacatagc
     1261 ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat tgagctttcc
     1321 aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct ttttcagtat
     1381 gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg ttattcaata
     1441 aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct acgatcatca
     1501 cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc cagctgattg
     1561 aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat tgagtcaaat
     1621 tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac atgatatccc
     1681 catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct tccaaatcat
     1741 gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt
     1801 tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc gcgattttat
     1861 caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga gcgccagcca
     1921 ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt tggatttgga
     1981 agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata cgtttagaca
     2041 tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca ccttttacac
     2101 cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga gagggacgtg
     2161 tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga tttcttgata
     2221 agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt ttccttcatc
     2281 tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa ctaaaacggt
     2341 gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc
     2401 caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta atgcccgtgc
     2461 aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt ccacatggct
     2521 atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt
     2581 ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc ggatcgttcc
     2641 agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat tatattgagt
     2701 catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt catataatcg
     2761 agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga ttgcgacgat
     2821 ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg ctccatgata
     2881 tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt
     2941 aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa tacggtcagc
     3001 tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa agctgatcaa
     3061 gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag tcatatctgc
     3121 tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga tcaatgtcat
     3181 cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg tataatcttt
     3241 attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct
     3301 gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct tttgcatact
     3361 tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca ttactgggat
     3421 gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta tgctcgcgcc
     3481 aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa ccatttggat
     3541 ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt tatcaaacgc
     3601 atcttttgtc agactctctg attttttata gacatccgta cgcaattgct tacccagttt
     3661 ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac tgatcaaaga
     3721 aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt gcgctacccc
     3781 tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta cttggatcac
     3841 catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga tttttagcat
     3901 ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt tcaaattgaa
     3961 ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg
     4021 cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca attcatgatc
     4081 attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg catcttgttt
     4141 gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag cgacttttct
     4201 tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat tctcgccttc
     4261 caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata gatcaccatc
     4321 ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg tctcaaagta
     4381 ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc tagggatatc
     4441 cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag aaaactcgat
     4501 tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt
     4561 cggcatatgg atcctccttt ctttcaagat gacacattgt catatgacgc tgtgtcatta
     4621 taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat catccgatac
     4681 attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc ccattaaaat
     4741 agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc caagacttca
     4801 ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc ttagattaca
     4861 caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca agacaattaa
     4921 aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg cgatcagaag
     4981 cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg
     5041 aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga ggctgtgaca
     5101 aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta ttcttcaacc
     5161 ttatcaatat gacttgatag ccttttcttt ttctcatagt tataacaatc atagcaataa
     5221 aaggagaagg taagcatatg gcatctgttt tagtagtcaa aggtcatccc ctaactgcag
     5281 aagaatcgcg cacagttaag gcattgacgt cttttttaac aagttataaa gaaaatcatc
     5341 cagatgatga agtgactgtt ttggaacttt atcgcgatga tattccggaa atcgatgaag
     5401 aattattatc aggttgggaa gcattacgtg ctggtgcaga attcacttcc ttatcagaaa
     5461 gtcaacaaca aaaaattgct cgtttcaatg aattgactga tcagttcctt gctgctgata
     5521 aaatcgttat cgcaaacgcc ttatggaatc taaatatccc aacaaaatta aaagcttggt
     5581 tcgataccgt caatgtagct ggaaaaacgt tccgctatac agaaaacggg ccagaaggat
     5641 tagtgacagg taaaaaagca ctgcacatcc aatctaatgg cggtgtatac aacggacaag
     5701 actttgcctc tcaatatgta aaaggtattt taaactttgt aggcatcgat caagtagatc
     5761 agctattcat cgaaggaatc gattatgacc ctgaccgtgc agatgagttg atgcaaaatg
     5821 cattagataa agctgctgct ttaggtaaat cattttaaaa attgagataa atgcatacaa
     5881 aagagacaga ggaaaaaatc ttcaaagcag catttgtacc ttggatattt tcccatctgt
     5941 ctctttttgt tattctttct gctttttcaa cggattttct tcataaagag aagagattgt
     6001 tgtgtaccat tcaaaaacat gagtaatcac tactttagct gctgcataaa caggaatccc
     6061 caagatcaca ccgatgattc cgaacaattt cccagaagtc agcagaacta ataaaatcgt
     6121 tactggatgg attgctaact gattccctag tactaatgga gagatcaagc gtccttctat
     6181 tgtctgctct actgcaaaaa cgatcagtac tttgatgatc atgaatggac ctgctacaat
     6241 ggctaaaaag atagccggaa tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa
     6301 aaaacccgca ataatcccca acgtgacagc atactctaaa ccaatgaccg caaaaccaat
     6361 cataaacatg attgccacag caaaagccac agttagttgt cctcttatat aagaagaaac
     6421 ttgactattc atttcactca gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa
     6481 tttgacaaaa taaggtgcta attgtttacc gtctttcaat agataaaata agataaacgg
     6541 catcgtcagc agagccacta aaatagtcgc taccgcgccg acaaaacttc caagactctg
     6601 aactgtactc ttagatatat cttggatcat atcgcctgcc gaattcatga acttatcacc
     6661 tgctgtttcc aactgttcgc gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa
     6721 tttattatca atcgtatcta catattgagg gaagttactg atgaagctca ctgtttgttc
     6781 ctgaatctta gggatgatca ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa
     6841 cagcccaatg atactgtaaa gccttggtac ttttcgcttt tcaaagtaat caactaccgg
     6901 attcatcaag taatacaaga ttcctgctaa aatgataggc agacctacga ttgctaaaaa
     6961 ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat aaaaagacga tcagcaatat
     7021 taacaataca acaagtaagg ctgtgactac ttgattattt aaaaaccagc gccaaaacca
     7081 cgataccggt ctgttcttct tattttttcc ttccataatt cctcctgtta gttataaaaa
     7141 atttggcttc ccaaaccaat agcaggcagt ctatgcggat gaagatagat tccatttgca
     7201 atttgtcctt cttctggaca agcatcaaaa atcaatgata cgtgagctac tgtctctaaa
     7261 ttcccattgg ccatcggacc gacataatcg atcgtatata cctgatcatc aaaagcaatt
     7321 gttccgcctg ctttcaaaga aaaaggttcg tcttttgata cttcttgaat caccgaatat
     7381 tttcgtaagg cagctgttgc tgttttatcg aacaaaatca gcatgggttc ttccttgttt
     7441 attgcttctg tcccaatttc tgtaatcttt gcctgaatca ttgcactctc tcctttaatt
     7501 gctttcaaaa acagtataac acattctcat tctttagccg tgtggaataa ttatgtgata
     7561 gaataaaaac gaaaactatt tcattacaat taggaggaat cagctcatat gatcgaaaag
     7621 ataattttag gaacctatac gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat
     7681 acagaaaaag aagaattagc caacctttca ttagctgttg aagaaaatag ccctacctat
     7741 gtagcaaaaa gtaaagctgg caatctttac acagttacta gtgtggatgg attaggtggt
     7801 gccggtgcgt acgataagga ttttcatttt ttgaatgcgg taacagagtc tggcgctcct
     7861 ttgtgttatg tagcggttga tgaagcacgt caattactat acggggcgaa ctatcataaa
     7921 ggtgaagtaa atgtctacca tatattagga gatggcgaac tgaaagcagc agattcaata
     7981 ttccatcaag aagcgacagg tcctcataaa aaccaagatc atgcgcatgt ccactatacg
     8041 gatctgactc ctgaccaacg attagtcgta tgtgatcttg gtacagatcg tgtgtatact
     8101 tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta ccttcactgc agagccagga
     8161 acaggccctc gccatcttgt ctttcatcca aatggacaat tcgcttattt atttggtgaa
     8221 ttagacagca cagtccgcgt gctttcttat aataaagaaa caggtgcttt tgaagaatta
     8281 cagaaaatat cgaccttgcc agaagaattc gacggtgaaa atggaggggc agctatccgc
     8341 atttcaaatg atggcaagtt cctttatgct tctaaccgag gacataactc gattgctgta
     8401 ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc aaatcatctc aacagaaggg
     8461 gattttcccc gcgatttcgc actagatcca agcaataatt ttgtcgtgtg tgccaatcag
     8521 aacaccgata atctgacatt atatcgccgt catcctgaaa caggattact ggaattactg
     8581 aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag
     8641 gagaatgaaa tgaatcagca agacgtatta gacagactac gggaagaact aaatattcct
     8701 ttctttgatg ggaaaatcga agataaagag tatagcgaag aggaatatca gaaactcaaa
     8761 gccgatttac agaactactt tgaacaatat gtacagaatg tagaaaacta atcagctttc
     8821 catactatct gaaaagaaca tcaggacaag acttttccgt ctatcctgat gttcttttct
     8881 acaaataaaa aaccgccgag atggttagac tcgacggaaa aggagtattt actcgatttt
     8941 gagtaagtac aaggttgttg ttggttatag gtatgtcatt atcataaagg attttgatca
     9001 ctctatcata aaggtaatat tacatttata ttagataact ctagttttta ttgttatcaa
     9061 agatttcttt tattttttca acaacaaatt agtcattttt tggatggcag ctgtcaattg
     9121 gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat agatcttcat ctacccctga
     9181 ttcaagatcg atactttgga tcatttcttc acagcctgct acatatgaaa gataagcttt
     9241 ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga cgcaaagtac tgactttctt
     9301 aagcattgcc cgatattttt ttgttccttc ttcaaataac aggatgatct cttcaagagt
     9361 cgatttgtcc agttcacttg tttttccttc atcaattgcg tttctgatca cttcatattt
     9421 cggatgcatt ccttctccga ttgcttctgt ttccttgacg atttgatcga tggactgcac
     9481 ataatagcca atattcggta acatgttcgt tttcctactt tctccattgt tgttattatt
     9541 tattctactt gtttttatcc tcaaatgaca agtacttgaa aaaatttaat ggtttctata
     9601 cagctcgcca ttcttattcg tgctcaaaga aaccatctgc taaactatat tgtttccctg
     9661 cgacaatgtc atattggatc aattgatatt cgtcgtaaat ctcttgttgc gagcgattga
     9721 attgcgcttc tttataccac tgtccgtttt gatagtacag ttctcgttca aacgccggaa
     9781 gttcctgttc taatgataaa agcagctgat aaaagccatt tgttgtctga ttcgtctgtt
     9841 ccattagatt cgctgcaaaa taaaacggac tggtgattgc atcgtgagtc gtttgctttt
     9901 ctaattctcc tttactgtcg aacataagaa attgtgtttc atgcaatgtt tgtttgtcat
     9961 tttttgcttg gatcgtatct gaatagatcc ctggaagatg atccccccaa aaaacaacta
    10021 aagtccttct agaaagattc ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat
    10081 aggaaatatc ttgcagataa ttctctaagt caagtgtccc acttccatct gaagtttttg
    10141 caggatagtc tagattatca tactttccat cgtagggcat atgtgtctgc atcgtgacta
    10201 aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt cattacttct ttgtaagctg
    10261 actcatcaga aatatatgga ttattctcta tcgtatccgt ataggtcatc gtacgctcgc
    10321 tgatgaactg gtcaaatcct aatgtctgat aaacatcctc tcttttatac atcgaagtat
    10381 tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt taacgctgaa acgatagaag
    10441 gaagctgatc catttttggt acaagcatcg tgtaaggggt ggtgagttgc gcattaaata
    10501 acgccataga aaaaccagtc aatgcttcaa attcgatatt tgctgttcca ccgccatagt
    10561 tttgagaaag catattgcca ctatatgttt gatcagctac ttcataataa tctgccaatg
    10621 gctcaccaga tacttctacg ccgtttaatc gactaggatc tgagaaactt tcactcatca
    10681 cgaacacgat gttcggcgac tcttcctcta cagttttatt tttttcatcg gctagcttct
    10741 ggtatttttc agtgatttct ttgatctttt ctttcgaata tccttcaggt tcatccattg
    10801 gttctacctt cagattgtac aaaaacccac cgataaaacc agtgttatag tagttcattt
    10861 tctggctgta aggtatccat agtgccgttt tattatatgc ttttcgcaac aaattatcgg
    10921 gattgttgaa attactgata taccctagca gtccaattgc cacaagcagt gtcgttaatc
    10981 ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa catgtaccag caaaacaatc
    11041 ctatcacggt tcctgcggca gctaagatca ctgtaaacag catagtgccg gtcatatctt
    11101 tcagcaatcc gatttcagtg atcatcttca aatcgtctgg ataaatcggt tcttgccgat
    11161 aactcatttt catataattt gcaaaaccta atacaccgat ggctacgaca tacgtaaggg
    11221 aacctaaagg gacagatcct gctagtgcaa tcagaaaaat aagaataatc agcaagacga
    11281 gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta
    11341 cggatagatc attttgacac cactgcaaaa ataaattact aaaaacaaca agaaaaaaga
    11401 tactgatgat tgctgctgtt ccttttaaaa tctttttctg tttggtcgaa agtctgctta
    11461 tatatctttt caaatgcttg ctccttcatt tctatcaata aaagaatgtt gcttctctta
    11521 ttttagttgt acgaaattcg aaagtaaaga acggaaagaa gaattaatga aatccaaata
    11581 ttctctttct ttatggcctt tctggtttct ttaataagcg gacctttcgt actccagaaa
    11641 ttttttctag ctgttgtttc acttgattga gctgttcaaa atcagtactg tctatgtcaa
    11701 tcaatatgta gacatgctca ttcttatggt tacgcgatat atctgcgatc gctagctcgt
    11761 tctcattcag cagagaaaat atctctgcta atatattggt tttcttttga taaaatatag
    11821 tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg ataattgacc gcttctctaa
    11881 ccgtaccgaa caaaagataa tcttttaagg cttcaactgc caaacgtccg ctgtctgcca
    11941 aagcttcctg agtcgatccc cctagatgag gcaacataag tatacggtca ttttctaaga
    12001 actcttcttc tggaaaatca gtcaagtatt tagccagatg ttgtttttcc aatgcttgca
    12061 taacagcata tttatcaaca atttcacttc ttcctacatt gataagtaca gcatcttttt
    12121 tcattttttc tatattcgcc tgatcgatca atccttttgt atcttctgtc aacggcagca
    12181 tgactacgat gtaatccgag gtagaaagaa ctgtttctaa atctgcttgc cgaacataat
    12241 ccaactgggc atcgcggata gaataaccca agacatccat ccctaagcta tagcaactta
    12301 gcgctacttt tgtgcctatc gctccaagac caagaagacc gatggttttg ccttgcaatt
    12361 ctcgtcccac ataagcactt cttttgtttt ctgcctgttc taaaatatta ggacctgtta
    12421 gtgtttgtac catacgactt gcttcgatta tcggacggga agataagagc aaacagcaca
    12481 aaacaagctc ttttactgca ttagcattta cacctggtgt attcatgacg atcgtcccgt
    12541 tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac accagctctt gaaattgcta
    12601 gcaaatctgg tgtaagccat tcatctttca ctttcgtact acgaataact attccttcgg
    12661 ctggcaaatc atctattatt ttgaaatatt ccccttgtaa cttttctatc tcttgttctg
    12721 gaatagcatc aatcaaatga acgtgataca taaaaccccc tccttactaa caagtatctc
    12781 gaaatttctg caattagaca actgtttacc atttgacttc ctagaagcaa aaagagcagt
    12841 tagagcagag taggctgagt gataaaaata aggctatgac aagttactgc cataagcctt
    12901 attttcgaga gcaaaatatt gagggtgaga tatcttgata aatggtgttc cgttagctga
    12961 cattgataat aagactaaat ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt
    13021 atttcttaaa actaagtggc tttttcacaa cctcttttta tctaataacc actgtttcca
    13081 actcaactaa ttttgcccac gtttcaattt gatctgttgt tagatttagc gagacaacgg
    13141 tatgatgacc gccacctgcc tgaatccatg aatggacacc ttcatggaaa ttcggtttag
    13201 ttttccataa cacacgtgca acaggtaaat taggtgcttc agtcgttggt tcaaaggctt
    13261 ctacttcatt gatcagcagt ttataatgcg taccgaaatc tgccatcgat acgactaccc
    13321 cttctcctgc tttcccatca aatacaagac gcgccggatc ttctctatct cccattgata
    13381 aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt cggatctact tccatcatat
    13441 gggattccaa gattgcttct tggccactag ctagctcgta agtataatct tccatgaatc
    13501 ctgtatcttt ccctcgagcc atgattttca gcaaccgatc aatcgctgca gttttccagt
    13561 cgccttcccc agcaaaaccg tatccttctg ccatcaaacg ttgtacagct agtccaggta
    13621 gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa agcgttatag cctccttctt
    13681 ctaagaaacg gcgaataccg atttcctgct gagcttgtac ttttacatgt tcctcccatg
    13741 tcttttcttc ataatcacca tagtcgaagg tatacaattc cttgtactct tcaaataatt
    13801 gatcgacttc ttcgtctgat acgcgatcga ttacttgtac aagatctccg ataccaaaat
    13861 aatcaacagt ccaaccaaat tgaatctgtg cttccacttt gtctccttct gtgacaccca
    13921 cgtttctcat gttgtcacca aaacgagcca cttttattcc aaaattatat gggattttag
    13981 attatctttt tagaagttct tgaatgtcgg tatagcccgt gtttccgggc ttttccggca
    14041 tttttgatat tgttagatac ttacaaatgt tcttattacc tctcaccatt tccccctcaa
    14101 aagtagtaaa tggagtagta aaaccggcct tgtgtcaggc tgccagccgt tccatttctg
    14161 cccttgcgga atcgaatgtg gcgtgtgcat aatagttcag cgtcatggtg atattggaat
    14221 gtcccataat gtactgcaag gctttggggt tcattcccgc attggctaac cgggtgcaga
    14281 atgtatgacg catagcatgg ggcgtcgtcg ttttcggcaa cgcttcttca tggctcttgt
    14341 tgtacttctt cacaagcctg ccaaacatcc cgccataatc taccgccgtc atgggatagc
    14401 cgttctgttt gagaaacagg aaattggcat acccgtcaat aatgaacggc tttgcacctt
    14461 tgcggttttt tatcactcgc tggaacgcct gatacacttc ctcattcatg gggatttttc
    14521 tgacgccgct ttgggtcttg ggttcgtcaa tatagtaacc gacgccggta tttctcaaaa
    14581 gctggtggct cacgatgatt acccggtttt caaaatccaa atctttgtcc gtcagtccgc
    14641 acagttccga aatgcgaagc cctgtcccca gcagaataac gattgcgtca taatgctttt
    14701 ggtaaacctt atcaccttga ataaaggaca gaaaactttc ttcctgtgct ggtgtaagag
    14761 gcacctttgg ttccgtatcg tcgtccagaa cgtcactaag ctggaagtca aacgggtttt
    14821 ttcggatgca atcgtcctgt atggcggtgt aaaaagccgc tttcagggaa cgcttgtcgt
    14881 tattgatggt cttataagaa aggccctttt ctttcatccg aagcgcccat tcctttgcgt
    14941 cggacagctt cacactgtca atcgggcagc tccccagccg atcttgttcc agaatacgca
    15001 tcagccgttc ccggcccagt ttggtgttgt gcttcacatt gccccggtgc ctgatctgct
    15061 tggcgtagag ctggcagacg gtcattttct tgccgatggt gtctatcccg tcgtcaaggt
    15121 ctttctggat ttctttctcc ttttcccgga gtgaaatatc ctcccgttta cctgccgggg
    15181 tcttatccgt aggcactaac ttccatgagt aaacaaactg cggttttcca aaggtatcaa
    15241 catatttgta agcatatcgt ccgtcttttc tctggctctc tccagaccgc aaaacgcgat
    15301 ttttgctgtc tcgtcttttt tctgacatct cgtcatgctc ctttccatga cggaaagagc
    15361 cgcatatatg cttataaatt attataacat atctgcggcc ctttttcact attagattgt
    15421 gtccaaagtg tcaattattt tctcaaactg cttgcgtttg atctggatgc ggttgccatt
    15481 cataatgacc cagccagagg ccggattttc ctcggccagc ttacgcaatt tgttttctcc
    15541 gatacggaaa tatttagacg cttcctcaat cgtcagcgtg tatttttccc aaataggcac
    15601 atcatggttc ataatgcagc cccctttcac aaattcaaag gtttgtgtga tttaggggca
    15661 tagacggacg gctgctggca cagctccacg ggactttcac ccccgcgtgg ttctcacgcg
    15721 gccctaccca ttgcctgcga cgcttttaac gctcggactg tggctataag ggagtatcat
    15781 agtcctgcct gtacgtcgtc gcccgcaagc cgccacgctc acattgcggc gtggtgttcc
    15841 tcgctccgct tttcccgtag ggaaaagtat catggcgcac cagccgtccg gctcggcaca
    15901 gacagaatgt gtccgtatgc cctatgatgc gccgccgtta tcttgcgggc atatttatca
    15961 aggtgcgatc aggccaccgg gaaaacaccg aaacggggaa agggtaagcc gtttcatgct
    16021 gccggggccg gttcgtcagt tcgtgacgat agcgtggatg agcttgattt ccagacggcg
    16081 cttcatggcc tcgtccagac acacatgcgg atagccgttt tcgtcataga gcgtccgggt
    16141 acacagcttg ttcatgtaac cgctgtaata ctgcaaaacg cgctctacgg ctccggcatc
    16201 cccagcgcgg gcggcctcga tcaccgaaag gggcaaaagc tcaatgccct tgtagttcgg
    16261 gttcttcatc cgcgtctaac ctcccttcgg catcaacgcc tttagctgtt tccgcagctc
    16321 cgataaggta tttgtcctgt gccgctgaac ggcactccgg gacattccca caaggccgcc
    16381 gatctcacca tctgccatat ccagaacaca gtgcaggatt aaaatactct gctccatgct
    16441 gggcagcgcg gcaaaggctt cggcaacaag ctcattgttg atatgtaggt catagccatg
    16501 cgacgaaaac gtaaagctgt cgctggggta gtgatctact gtacagagct tgtccaaatc
    16561 tgcctgtgac aaagagctta gtgacacttc acggtcacgg cggcgcttca tttcagccaa
    16621 gtagcttttg gcctcgttac gcagtaccgt cttgcaaaaa gcatcgaaca tgcaccgcgc
    16681 ttcatagtcg cgggggatag catccatctt ctcaccccct ttctgtgggg gacttaggtg
    16741 gttctctccc tttccgctaa caagactttg acaaggccgc cagctacccg ctaaatacct
    16801 cctttttgaa aatttctcaa aaaatttttt tggggacagc cgggccgcaa aagacgacaa
    16861 aaatcgcccg gcaggtcgca aacctaccgg gcgagaaaat gctttgctat gctgctgatt
    16921 tacatggtat agtacatact cataaccgga atagccctgt g
//
LOCUS       DAZULG010000061        16082 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000061 DAZULG010000000
VERSION     DAZULG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.063"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            222..890
                     /locus_tag="ACG9CY_001768"
     CDS             222..890
                     /locus_tag="ACG9CY_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS4938273.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1090..1410)
                     /locus_tag="ACG9CY_001769"
     CDS             complement(1090..1410)
                     /locus_tag="ACG9CY_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938274.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1447..2829)
                     /locus_tag="ACG9CY_001770"
     CDS             complement(1447..2829)
                     /locus_tag="ACG9CY_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4938275.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2985..4172)
                     /locus_tag="ACG9CY_001771"
     CDS             complement(2985..4172)
                     /locus_tag="ACG9CY_001771"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS4938276.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4179..5210)
                     /locus_tag="ACG9CY_001772"
     CDS             complement(4179..5210)
                     /locus_tag="ACG9CY_001772"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4938277.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5256..6680)
                     /locus_tag="ACG9CY_001773"
     CDS             complement(5256..6680)
                     /locus_tag="ACG9CY_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS4938278.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6855..7760
                     /locus_tag="ACG9CY_001774"
     CDS             6855..7760
                     /locus_tag="ACG9CY_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS4938279.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7851..8528
                     /locus_tag="ACG9CY_001775"
     CDS             7851..8528
                     /locus_tag="ACG9CY_001775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4938280.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8525..8695
                     /locus_tag="ACG9CY_001776"
     CDS             8525..8695
                     /locus_tag="ACG9CY_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938281.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8809..9705
                     /locus_tag="ACG9CY_001777"
     CDS             8809..9705
                     /locus_tag="ACG9CY_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4938282.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9760..11130)
                     /locus_tag="ACG9CY_001778"
     CDS             complement(9760..11130)
                     /locus_tag="ACG9CY_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4938283.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11368..12114
                     /locus_tag="ACG9CY_001779"
     CDS             11368..12114
                     /locus_tag="ACG9CY_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4938284.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12111..12950
                     /locus_tag="ACG9CY_001780"
     CDS             12111..12950
                     /locus_tag="ACG9CY_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4938285.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12951..13901
                     /locus_tag="ACG9CY_001781"
     CDS             12951..13901
                     /locus_tag="ACG9CY_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4938286.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14042..14794
                     /locus_tag="ACG9CY_001782"
     CDS             14042..14794
                     /locus_tag="ACG9CY_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938287.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14813..15964
                     /gene="queG"
                     /locus_tag="ACG9CY_001783"
     CDS             14813..15964
                     /gene="queG"
                     /locus_tag="ACG9CY_001783"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS4938288.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5220 a         3001 c         3232 g         4629 t
ORIGIN      
        1 caatttcgta aaacaaccgt aacggttgtg taaaaatatc attttagagc ataaaaaaaa
       61 ggagtggatt tctccactcc aaaaaagtct aacttttggg ggtcactaca gtgtcacagt
      121 ccttttttta gtaaaaggat acatttcttc cttgtatatt ccatgcgtgt tcgtgagaaa
      181 gcatatataa tttaattaac agatagttag ggggagaaaa aatgattttt tcaattacaa
      241 tgaaactggt gttcgggttg atcggtttat tattagtggt acgtttgctt gggaagaaat
      301 ccatgtcaga aatcacaccg tttgatttag tctatacgct tgttttaggt gggatcttag
      361 aagaatcaac ctatgatgac aatgtccatg tagggcatgt cctatttgcc attgcgcttt
      421 gggcagtaat gatttatggt atcgaacgta tcgtccaaaa aaatgaaaag gtcaatcgtt
      481 gggtcaaagg cgagccatct gttctaatca aagacggcgt catcaacatg acagagttga
      541 caaataacca tatcgagatg gagcagttgc gagcaatctt aagacagcaa gaatgctttt
      601 ttcttgaaaa tgccaaacat gttatcctag agaatgccgg tcaaatgagt gtattgaaaa
      661 aatcagatga agacaaagct ttatcgatcc tgcttgtaga tgaagggcaa attcagcata
      721 aagtgcttca gtccaatcaa ttgactgaag catggcttat ggagaacttg aagaaagaag
      781 gctatgcaga tgtaaaacag ctgatttatg ttgaatggtc agaagaaaaa gggctctaca
      841 tactaaataa agaagacgtc atgcatgaat cttaccgaat agatggatag atagacagag
      901 gttgtgaaat cagcgccttg gcttgttact caaaactaag cgactttttc acaacctctg
      961 atatctaata tacgttgttc cagaagttgc ttcttcgaaa aataagccaa aattccacaa
     1021 aaatttaaga agcaattttc gaaaaatcct ttcttatttt ttgaagttga tcacttctgt
     1081 cacaacctct catgtcacaa accatttttt tgtttccttt aactcaatat accgtacgtc
     1141 ttcccacaat atcggctgat ggtcaatcat aaagtaatct tcataggctt caccaatgaa
     1201 gtgcccttcg acctcatcca agagtcttcc aaattcatct tttaaattga gctgaatgat
     1261 tactgcttta cgagataaaa aggcttctgt cagtatacta tcgatttctt gcaatgtcat
     1321 ttgttgttta ggaagaggct gtttgcgtgc ttcttcttca tttttttgat accgttgact
     1381 agttcttcca tcgcaaaagc agtcccccat tttaatccaa atggccgatc tcgatactcg
     1441 ttatagtcaa tgaatttttt tgctgtccgt ctcatctttt ccaccttcta atccactcat
     1501 accacctgca tgcccgccaa cgagagaact acgtgcgatg gcgcgtcctc cttgcaagag
     1561 actatgcgca tggatgattg attgaaagcc gtattttttt ctaatcgtat cgacaacaaa
     1621 atcaactttc gcttcattta tctgcttttc aggatcttca aaaaggttaa gctgtaaacc
     1681 tgtcgcgtat acgaggtcgg aacagttcac gcctacacgc cgaatatcct ggtagtggta
     1741 atgctggtgg aaaatggtca ataaagcatc tgcaagatct ctgctagcat ttgtcgcttc
     1801 gattttcatt tgctggtgaa acccggtctt gccatttgga tcaagatagc ctaaagagta
     1861 tcctacccat aagctgacta cttgtgtttt tgctgcagat cgtctcaagc gagtgccgac
     1921 ttggtcggcc atttctttga taacgatctc gatttcttct cgtcttgtat aatctctatc
     1981 taggatttga ctatttccga tactttttga ttttggtgta tattcttgtc ccaaaaaact
     2041 tcgatcgatg ccccaagcat gggcataaag ctgagtaccg atgaccccta gcttgctttt
     2101 caagtgataa taatccgcgt gagctaaatc ataaacagaa tgtataccca tttgtttcag
     2161 acgttttgct gttcgtttgc cgattcccca aaagtcagtg atatctgaga ttttccataa
     2221 cttttctggt acatcttcat aacgccattc agcaaacatt tgctttccgt cttttgagcc
     2281 attgtctaag gctagtttag caagtagcgg gttatcgcca attcctatag tcgtgtagat
     2341 tccggtctgc tcaaacacat ctttatggat cagtttagcc atgtcgtacg catttgtttt
     2401 accaaagagt gccatgctat ttgttacatc aaggaagctt tcatcgacgg aatagacatg
     2461 atggttcgct tcgtctgcat attttttata gatagaattg atttctctgt tcttttccat
     2521 ataataggcc atccttgggg caacaatata taaatcactt ggatagggga agggaagatc
     2581 tctggcacgg ctgatattgg tgatgccgta agcttttttt gctgcaggac tggaagccaa
     2641 gattaatccg ctaccccgtg tttctgtcga atcggacgga taggacatga ctactaattt
     2701 agttgtaagg ggatcaagat ccttttcaac gcactctacg gaggcgtaaa aagatttgca
     2761 gtcaatacat aaaatatctc ttgaaggttc tttttcataa acaaataccg gattttgatt
     2821 cgcccacata acaatcacct cttcaacaca attagaacac acgttctctt tccgtgtcaa
     2881 taaaaaacag caattttcaa atacgagcaa aaggctcaca tttggaaatt gctgagaggt
     2941 tatttttatc tatttgtttt tagctgagat cgttgatttt tttatcaaac atgaacagct
     3001 tctggatcga tctttgttgt cccatttcca atgtgtgcaa cataaaattc cggagcatag
     3061 ccgacaactt tcatgtattc atcatagaca ttgtttttga aggcaggtat attttcttct
     3121 ttgacaagag caatcgcaca gccaccaaat ccagctcctg tcattcgtgc acctaagaca
     3181 ccttcttgtt tttgagcagc atctactagt gtatctaatt cgatcccagt tacttcataa
     3241 tcatagcgaa gagagtggtg agaatcattt aacagttgac caaatgcttc taagttccca
     3301 gcttctaatt cagcttttgc cttcaaagtc cgctgatttt cagaaacggc atggcgagct
     3361 ctgcgtatca atgtatcatc gccgatttgg tcaatagttg attcaaattc ttcttcatct
     3421 aagtctccta aagaagagat aggcaaagtt ttttgcaaac gtgccaaggc ttcttcgcat
     3481 tcgctacggc gttcattgta ttttgaatct gccaattccc gccgcttatt cgtattcatg
     3541 atgacgattg cataaccatc tagtttcact ggaaccattt cgtatttcag cgtatttgta
     3601 tctaataaga ttgcatgatc tagctcaccg aagccgatgg caaactgatc catgatacct
     3661 gaattgacac cgatgaattc attttctact tttttacctg tttggactag ttccaagcga
     3721 tctgttttta aatcaaacag atcttctaat acaacaccta ctaataattc taatgaagcg
     3781 ctactggaaa gtcctgcacc attagggatt gttccttcca ctaataactc aaacccatga
     3841 tcgattgagt gtccagcttc ttttaattta aggaccattc cttttacgta atttgcccag
     3901 tttgcttttt tatcatattc tagctcatct aaagtaaaac taatgacacc gatatcgtca
     3961 aagtttgtgg aataaactaa tattttatta tcgtctcgag gtgctgctac accttgcgtt
     4021 ccataagtga tggaagcggg gaagacatgg cctccattgt aatctgtgtg ttcaccaatc
     4081 aaattgatgc gtcctggtga gaagtattgt gtagtttttt taggtccgaa tgtttcggca
     4141 aattttgtgt ttaagcttct tttgatttcc atgatgagtt attcctttct tgttttgatt
     4201 ttgaattctg ttactgtttc aaatactgat ccggctttta aatgaatcga accaaatgct
     4261 gagaagcgct ctgcacctgg agctgtttga gtctcaaatg tgatgccacc gtgatgtgcc
     4321 agttttcggt tgcgcatttc tggtgtttct gtgccgaaat ttgcagtgaa aataaccaca
     4381 ctagaagcat ctgtagcaat atctacttgg attcttttat cggggctagt caagcgagcg
     4441 gcttctttac ccaagccggt ttcttttaag aaaaatggat gatcgattcc gtcaacaaga
     4501 tttttttgat ccaaatcact agcaaaaacg gacgctaatt ttttaggtat ttgaaaatcg
     4561 aaagcacttc ctgcagcatt ttcttttacc cctataggaa tactatctgt tcgcaaagga
     4621 gcataagctt cgctattgag ccaaagctca tgttgatcga ttgactgaga agcatctccc
     4681 gtcaagttga agtaaacatg atttgtcgga ttgaataaag tatcttggtc actggtgccg
     4741 cgagaggtca ctcgccagat attatctttt gtcaaagtat agcggatttc cacatccatt
     4801 gtacctggaa aaccatgttc tccgtcggga cttgtagtag taaaaatgac agaagcttca
     4861 ttttcgccat tcaaaatgac ataagaccat ttttttaatt caaatccagg agcaccacca
     4921 tgcagattat gtccagtttt tggatctgtg gctaattggt aagtttttcc attgagagaa
     4981 aagcggccat tttcgattct ccctgcggtg cgaccgatag aagcaccgat atacggatct
     5041 ttttcgatat attcttcagc ggtgtcaaat cccaaaacta attctcgatc atttgatttt
     5101 aaactgacga ttctagcacc taaatcagag atagaaagtg ctagcttgtt tttgttctcc
     5161 aaggttatca agtgataacc ttggccaaaa tctttttctg taatcttcat tttcaagatc
     5221 ctttcttaat gggaaggaac tactgcatcg tgaagttaat cttccattgt atcagctgga
     5281 gccaatttgt tttccaattt ctcaacgatt tgtgcatgtt ttttctctgt caatgtaatc
     5341 ttccaagcaa aaataatagc ggagaaaatc atcaaagcag caggagcata gaatgcataa
     5401 gctttgaaag tcgttacgcc atgagcagtg atatcagcag cagtagcatt accagtcatc
     5461 ccagaagcga tagcgacaaa ccctacgatc ccgttagaca aagcaccagc gattttatct
     5521 agtaaagggc gaattgatag agtaacagct tcgttacgga taccattttt ccattgacca
     5581 tattcaacag agtctgtgat cgtcatcaaa gcggataaga aaatcaattg ttgagggaag
     5641 tagaacaata ctagcccgat gataacgatt ggcaaacttg aacctgcgat cgtaaagaaa
     5701 aagtaagaag aaatcatcat tgcgatccct ccaaggtaaa cgtagcgacg agagatgacg
     5761 cgtgtaagca ttgggaatag tggaacagct actaaaccaa gtaaggcagc aactagacca
     5821 acgatccaaa attcagttgg ttggccaagt acatatttga agaagtagaa taatacggca
     5881 gttgttgctg cattggcaat cgcataagcg atataagcaa gggcaagcca catcaactga
     5941 tcattttgag caactgcttt gaacacatct ttgatcccag ctttttcatt ttgctgacgg
     6001 atcgcagtat cgacttcttt tgtgcccaag gcagtgataa tcgcagtacc gcctgaaata
     6061 acagcagcaa tcacaccgaa ccagaaccag cctgaagcgc cttcaatatg tttatgagta
     6121 acaagatagg taaagaaagt aacgataggg acaacgacta gagtagtacc atttgcacct
     6181 agagaagagc cgaaacgagc aaacgtagca aatttttcac gttcctttgt ttccgtactc
     6241 aaagctggaa tcattgacca aaaagcgata tccttaaatg aataaaaact atccaataag
     6301 atgaaaacga ttgcaaataa tatgataaat aaagtcgtat tatgagtagc taagccaaag
     6361 aaattggtaa agattgctac caagagtacg gaactaacaa cgccgccgat aactaaccat
     6421 ggtttgaatt taccgtattt tgtacgagta ttatcgatga tgccaccaat caaaggatca
     6481 aaaataattt caactaagcg gatacctaca actaagcttg taactgtagc aatcattttt
     6541 gcattcgttg ccttatctgt tcctgcgaac atctggcttg tcacgaatac cataaaataa
     6601 gtgcttaatg taacatagaa tgtgtcgtgt ccaaaagcgc ctaaagcgta ggacacacgt
     6661 tggatcattt tgcttttcat aatctgtatt cctctcctgt tcaatatata atactaagta
     6721 aaccggttac aacgaataac aaagatcttt taatgactta aacttaacaa gaaatcgctt
     6781 cctttttaat atatttctgt gacttttatt taactttttg ttgcattata ctaaaggagt
     6841 aaggagggat agacgtggag caatcgattt ttttaaaaaa ggaacatcaa caagtagaaa
     6901 aagaattgta ttttgatttt tgtggtttct ctaaaacgat gccgtttcat tcttttggtc
     6961 ctgctattcg caatgattac atcctgcatg tcgtgatgga aggcaaagga tcttatcatg
     7021 tgaaaaatca acaattccaa ttgaaaaaag gtgatctgtt cttgattcgt ccaggagact
     7081 cgacgtttta cttatcagat ggagaagatc cgtggatgta ttgctggctt tcatttggtg
     7141 ggagtgctgc agaagaaatc attcggcagt cgcttttcag agaagaccaa tacacgatgg
     7201 tctcttctga catacaaaaa tatatcgaca ttattttaga atgcatgaaa gatacggaag
     7261 aaaccttgga aaatgaatta cagttaacag cattaaccta ccgtttttta tcagtccttt
     7321 taaaagatgg cggaaaggtc agcggtggtg tacaaaaggt tcattcctct ttagctgttg
     7381 aaacagtcaa atacatcgag gcacattatg ctgaaaaatt gtctgttgag gaaatcgccc
     7441 agcatttagc ggtcaatcgg agccatttgt cgcgagtatt caaaaatcat atgggtacca
     7501 gtatcaagga atatttgatc ggggtacgga tcaatcgagg ggcgtttctt ctttctctga
     7561 cggatgaatc cgtggaaagt attgcctatc aagtagggtt caatagtcta gtcgtattta
     7621 gccgaatgtt caaaaagagt accggagaaa cggctacgaa ctatcgtaaa cgtatgaaaa
     7681 acgaaaattt taagaatatt gctttggata ccttgaaaaa acagttggaa aaacaaaccg
     7741 cagtttcttg gacaacctga ttagtctggt taaaatgaca agcatcggca aaagaaactt
     7801 gtggcggtta tgctacactg taaagcgaag actaaaaagg aggaaaaggg atgcgtacac
     7861 ttatcatcat tagccatcca acgatcgaag aatctttaaa tcagcaattt ttcaaagaag
     7921 cttctgctgg tttaccagct acttggcatc atttggaatc aagttatcca gacggaaaaa
     7981 tagatataga gactgaacta cagctcttgg cccagcatga tcggatcatt ttccagtttc
     8041 cgttttactg gtatagttct cctgctcatt taaaaatttg gcaagataat gtcttggaac
     8101 gagcagcaca tgtattaaaa gggaaagaac taggaatcgt attgacaaca ggtgtggcgg
     8161 aaaaagaata ccaagctgga ggaaaagaag aatatacgat ttccgaattt ttgaggccgt
     8221 atcagcgaat cgcaaataaa ttccatatga cctatcttcc gccattcgtt ttagcacaat
     8281 ttatgtattt atcccaggaa aaacgttggg agaagctaat tgcttatcag cagtatctct
     8341 cattggaagg caaaccatcg ctgactcagc ggattgactg gtttattcaa agagtacagg
     8401 aaaatcagaa aatgcaggaa gaagaatctg aaaaacaagc atatatcgta gaagcactga
     8461 ctgaggcaaa agagcagatc gaagacttga cctttacact gcaagaaatg aaaggaactt
     8521 cattatgaca aatgattggc tgatccaaca attagatgaa ttaagcgaaa aatcagaata
     8581 tcaagaccgt gcgctcttat atgaaataaa gaaactgatg gctgagatag acgaacgaac
     8641 aatgcagctg caaggagaaa tcgacggaca atcttggaat catctcaact ggtgaaattt
     8701 tcttataaat aacaaagata aaaatgtgca cagcgacaat tatttgctac aataagaata
     8761 tgaagcgttt gataacgcta ttatacataa aaattggtgg tgaaaaaaat gtatgatgaa
     8821 aatgagtggg gagaaaatgt tcggatgacc cgaagaagac agcaaaaaca ggcagctcgt
     8881 aagcggatga tcatgattgg ggcagcagtt gccgtgtttt tgatcatagc aggaggagtt
     8941 gcgggtctat attttcatgg aaaaaattcg tcagaagcga aaagtacgac cataacggaa
     9001 acaacgacaa agaaaacaga gacgaaacaa acaagtgagt catcaactaa aacaaaagag
     9061 acacaaactt ctgaagaaaa aacagcaaat ggtttgccag agcaacttca agcaatcgta
     9121 aatgattatt cgaaaaagat cgaagaaaag acgcctgcat tgattgaaga ataccagaca
     9181 gaaattcagg gaaatcaaga gggaatagct ggtttatcag cagttgctaa ccaaaaagca
     9241 cgtgagcttc aagcaatctc cgacgaaggg atcagaaaat tacgtgcagc gtatcaggca
     9301 gcagaaaata aagacggtgt agacttagat actttgatca atcaattatc ggctaattat
     9361 acgaatcatg tcgctaagat ttccgatatt tatttgcaga ctagcgcctc tcttcaagca
     9421 gagtcaacaa gcacacaaga cacaacatct tctgattctg agacagcaga gtcggtagaa
     9481 gcttctgaaa tgcaagctcg aacaacacaa gacacaacag attcctctga aactaatacc
     9541 aacagcgcga cttcacaggc tgcgacaacc gtagttttgg aaggagaagg tccaaatcaa
     9601 atagccgaac gaactggcgt accagtagaa acgatcttgt cgctgaatgg gatgagcatg
     9661 gagaattatt tcctgactcc cggtgagacg ctaaaattga attgataaaa gcaataaaaa
     9721 agaacgaagc ttccactaag gtgaagcttc gttctttttt tattttccgg ttcttttttc
     9781 gcgtacaaag taatggatcg tcatgtaaag caagaaaacg agtccaataa tcaatgagat
     9841 tctcgtttca ggattgataa acatgaaaat caatacgata gctaaaaaag caagtgtcac
     9901 ataattcgta taaggagata gtggcatttt aaaaggatga tcatgcattt tatctgcgtg
     9961 cttctttttg aagcgaatct cgctgatcaa aatcactacc caaggaacca taccaggcaa
    10021 aatacttgag ctgtatacga aaacgaaaat attttctgac tggttgatga acattggtag
    10081 aagataattt aagatcagac caattaagat tccacctgag attgctacga caggataaaa
    10141 aggcgcaccg tgacgagata gtttcagaaa ttttttagac atctttccat ttatggcaag
    10201 tgtatagacc atacgacttg cgctgaaaat ccctgaattg caacctgata atgccgctgt
    10261 aatcacgaca aaattaatca gtcctgcagc aaatgtaatg ccgactttag aaaaagtttg
    10321 tacaaaaggt gaaccaatct cactcaattg attccacgga tagatactca caatgataaa
    10381 aatagcacca atataaaaaa tcaagattcg accgatagtg gactggacag cttcaatgat
    10441 cgtcttttta ggattctctg cttcaccagc agtcattccg atcagttcga ttccttgata
    10501 ggaagcaaca acgatcgata atgcaaagaa aaatcctttg acgcctccag tgaagaatcc
    10561 accatttttc caaagatttg aaatccctac tgcctgacca tgattaccaa atccaaataa
    10621 gattacccca agacctgcaa tgatcatcaa aatgatcgtt atgactttga tgatcgaaaa
    10681 ccaaaattct aattcgccaa acgccttgac agaaataaga tttgccagca taagaaacaa
    10741 gacgacaatg attccgggaa taattgccgg caaatcaggc caccaataat tcatataaga
    10801 tcctacagca atcacttcac ttattcctac gacaatatat tgaaaaatat tactccaagc
    10861 ggtcaagtag ccagccacag gatggatgta ctcgctcgcg taatttgcaa aagaaccagt
    10921 cgaaggatca acgtaaagca tttcgcctaa agcacgcata atcaaataca aaaagaatcc
    10981 tgcaatcgca taggctccca tcactgaggg ccctgtccaa cgtatggttg aggaggcacc
    11041 cataaacaga ccgacaccga tcgttccacc tagtgcgatc atttggatat gacgggcact
    11101 aagacttcga gttaactttt cttcttccat acaaaaactc ctttcgttgt tgagtcaaca
    11161 agaaaaaata atcaaacgtt tcaacacatg tttttcatta ttaggttagc cttattgaac
    11221 tcttgatgtg attctacaga aaattttcat aaaatgcgag gaaaattcga aaaaaacttt
    11281 ttcatcattc tacttgaaaa tgctttccat tttagttaaa ctatatttaa ataaaaagtt
    11341 aggtaaacct aaaaaggagt gtaagatttg gaacgtacgg ccattgtaat tcaagacctt
    11401 tcagccgctt atcaagggaa aactgttttg aaaactatca acttgacgat aaatccacaa
    11461 aaaattaccg gtattatcgg accaaacggt gcaggaaaat caacatttat gaaatctttg
    11521 ctagagttga taccagctac tactggtaaa gtcacctttt ctggacagtc ggtaaactca
    11581 gtccgtaaaa aaatagccta cgtggaacag cgaagtgaat tggatctttc ctttccaatt
    11641 gatgtattag gcgttgtact tttaggaaca tatccatctt tacgaattgg acaaagacct
    11701 gggaaaaaag aaaaagaacg tgcaagacaa gctttgaaaa aagtagggat ggaagaatat
    11761 gccaaaagac agatcagcga actatcgggt ggacagcttc agagagtttt tattgcaaga
    11821 gctctagccc aaggagcaga atggatcttt ttagatgaac cattcgtagg gattgatgcg
    11881 ttaagtgaac gaaagatctt tgacatcttg caggaattga agaattcagg aaaaacgatt
    11941 ttgatcgtcc atcatgatct tcataaagta gaagaatatt tcgatgaggt tattctttta
    12001 aataaacagc tgatcgcttc cggtccagta caagaagtct ttacatcgaa gaaccttcaa
    12061 ttggcctatg gtgagatcat cagacattta gtaaaaggag gagagaaaaa atgatccaat
    12121 cctttattga tggattaatg aattatcaat tcctgcaata cgctttggtc acttccatgc
    12181 ttgtaggact tgcttctgga gtcatcggtt cttttattat tttacgaggg atgtctttga
    12241 tgggagacgc tatctctcac gcagttcttc caggtgtagc catttcttat atgtttggtt
    12301 ttagttacat ctttggagcc actgcttttg ggatgctaac agcagcagtg attggtttcg
    12361 tgactcagcg gagccgattg aaaaatgata cagcgattgg aattgtcttt agctcctttt
    12421 ttgcattagg gattatcttg atttcatatg cccagagtgc gacagatttg tatcatattt
    12481 tatttgggaa tgtcttagct gttagggaaa gcgatttgtt gctgacagca ctcgttagtg
    12541 gaatcgtctt gatatttgtc tttttctttt ataaagaatt gaagatcaca tcattcgatc
    12601 cgacaatggc aaaagcttat gggctgaaca catcgctgat ccattatctt ttgatgttct
    12661 ttttgacatt agtggctgta gtcagtttac agacagtagg aacaatcttg gtgattgcca
    12721 tgttgatcac accagccgcc acggcttact tgctaacgaa ccatttactg aaaatgatca
    12781 ttacagctgc aggaatcggt atgctaagtg cagttgtcgg tgtgtttttc agttatagtt
    12841 acaattggcc atcaggagct acgatcgtgt tagcatgtac cgcatttttt atccttgctt
    12901 ttttattttc tccaacaaaa ggaattttat ttcagaaagg aagcagatag atgaaaacaa
    12961 aaaaatcact ctttttaatc ttggcagtta gtttcttggt attagccggt tgcggaaagc
    13021 aggcttctga ccaagcggat gaaggaagta aagaaaagtt gtcagtcgtg gctaccaatt
    13081 cgatcttggc ggacatggca aaagaggtcg gaacagatca aatagatatc cacagtatcg
    13141 taccagtcgg aacagatccg catgaatatg aagtactacc agaagacatc aaaaaggcaa
    13201 gcgatgcaga tgttatttta tacaacggtt tgaatcttga aacaggtaac agctggttcg
    13261 ataacttgat ggaaacggct aaaaaagaag aaggaaaaga ttattttgca gttagcaaaa
    13321 atgtagaacc tctttattta actagcggtg aagaacatac aaaagcagat ccccatgctt
    13381 ggctagacct atctaacgga ataaaatatg tggaggaaat cgcacgtata ttctctgaaa
    13441 aagatgcaga aaatgcgaca ctctataaaa aaaatgcaga agcatatgtg gaaaaactaa
    13501 aagaattaga tacacaagcg aaggaaagtt ttgcttctat cgaagagaac aaaaaattat
    13561 tagtaacaag tgaaggggca ttcaagtatt tttcacgagc atatgatttg ccagcagctt
    13621 atatatggga gatcaataca gaaagtcaag gtacgcctga tcaaatgaaa gcgatcattg
    13681 atcagataag agcaaaagaa gtaccggttt tattcgtgga aaccagtgta gactcaagaa
    13741 gcatggaacg ggtagcaaaa gaaacgggtt tgaaaatcta tgataaactg ttcactgatt
    13801 ccatagcaaa agaaggagaa caaggagatt cttactatca gatgatgaaa tggaatattg
    13861 aaacgatcca tgaaggtttg tcacaaacaa aagaaagctg aaaagtgcaa ggtctagctt
    13921 gaggagcaga ccttgcattt tttataagag atatgaaaaa aaattatgct gtttttcgtc
    13981 gtacagatac cttttttgaa gaaattccgt tatagttaga gtaaggaagg aaggtgtggc
    14041 tatgaatttt acacgtaaag ataaatatac gattaaagaa gaagtctttc gattgatcag
    14101 ccagcaggaa aataccgaca aggatcaaga ccttcaattt cttgaagcag aattagctaa
    14161 attattcata gcaaatcaaa atgagacaac agaaccagat gaagagctgt acataggcaa
    14221 atggttgttt gatgaatttc agcctttaga tgaaaatacc ggtatcggtt attttgtgtt
    14281 cctttctcca gtcggcttca cacctggcgt attagttaaa caaaacgacg aaataaccgt
    14341 caaagcatgg cctgcattat tttcagtcat caaagcagaa ggagtaatca aaaagatcca
    14401 gtcggcctat tataaaagtc aatttattgc caacagcaaa ttaatgaata tgttttataa
    14461 tcgtctagta gaaattgcta ggcctgggaa aatcccgcaa acattcacgt acacacagca
    14521 aaaaaatctc tatgaagaag tattgtattc ccgcgatctg tctgagtttt cgccatggga
    14581 agaggtaagg ctccatgaac tgttaagtac aaaaccagtt tcattgaatt tttttaatgc
    14641 attagataac aaaagaaaat taatttcaac aaattattgg gaagtatttg ctgcaagaaa
    14701 aaaatggatg gaagaaaaga agattgccga tcagcagatc cagtttgtcc agctgaaaaa
    14761 agaaaaacta tattatgggt tatacagccg ttaacagcaa gcgaggaaga ccatgacatt
    14821 aaaagaagaa atcatacaag aaagcaaacg attaggaatt gacaaaatcg ggttcaccac
    14881 ggcggaacct tttgattcat taaaagaatc gttggaagaa caaaaagcag ctggccatac
    14941 ttctggcttt gaacatccaa atatcgatga gcgtctttat ccagaaatga catttgatca
    15001 gccgaaatcg atcatttcca ttgcacttgc gtatcctact aggatccatg aagaagtgcc
    15061 tagggatgaa aaacgcgggc agtttgctcg tgcttcttgg gggatcgatt accatgatat
    15121 actcagagac cgtttgaacc gattgatcga attcatccaa acaagagcag aaaaatggca
    15181 aaaagaagag gaatggcgtc tagctccgca agtagatact ggagaactga tcgatgtcgc
    15241 tgtagcacag cgagctggtt taggatttat tggacgaaac ggattgttga tcactgaaga
    15301 gttcggttca tttgtctatc ttggtgagat cgttactaat attgtatttg agcctgataa
    15361 accaggagaa tttggttgcg gtgactgtat gcgctgcgtg actgcttgtc cgacaaaagc
    15421 attgcttggt gacggaagaa tgaatgccca gcgatgtttg tcttatcaaa ctcagacaaa
    15481 agggatgatg ccggaagaat atcgtaaaaa aatgcgaaat gtcatttatg gatgtgatat
    15541 ctgccagtta gtctgccctt acaatcaagg aaaagatttt cattttcatg aagagatgga
    15601 accaaaagta gacgaaatat atccaaaatt aaagccaatg ctttcgatgt ctaataaaga
    15661 cttcaaacag caatttgggc atttagctgg ttcatggcgt gggaagaaac ctttgcagag
    15721 aaacgcattg attgctttag ctaatctagg tgatcgaacg gcattgcctg agatttcaga
    15781 atgtttaaca gatgtccgcc ctgtcatcag aggaacagca gcttgggcta ttggacgtct
    15841 aggaacaaaa gaaccggaaa aatggcttga tgtattagat gaaatattag ctaaagaaac
    15901 agaagaagtc gttataaacg aattggaaca tgcccagaaa ttattgcgga gaaagttaaa
    15961 ataaaaaaat agttaacatt attcaaagtt tttgtccagt aattgttgta acaattactg
    16021 gtttttttgt attttttaca tttttttaag agaatttgat aaaaatcaaa aatttttttg
    16081 ct
//
LOCUS       DAZULG010000062        15883 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000062 DAZULG010000000
VERSION     DAZULG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.064"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..863
                     /locus_tag="ACG9CY_001784"
     CDS             111..863
                     /locus_tag="ACG9CY_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4938289.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            860..1774
                     /gene="pfkB"
                     /locus_tag="ACG9CY_001785"
     CDS             860..1774
                     /gene="pfkB"
                     /locus_tag="ACG9CY_001785"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4938290.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1792..3723
                     /locus_tag="ACG9CY_001786"
     CDS             1792..3723
                     /locus_tag="ACG9CY_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4938291.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3834..5393)
                     /locus_tag="ACG9CY_001787"
     CDS             complement(3834..5393)
                     /locus_tag="ACG9CY_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS4938292.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5548..7782
                     /gene="pcrA"
                     /locus_tag="ACG9CY_001788"
     CDS             5548..7782
                     /gene="pcrA"
                     /locus_tag="ACG9CY_001788"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS4938293.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7799..9835
                     /gene="ligA"
                     /locus_tag="ACG9CY_001789"
     CDS             7799..9835
                     /gene="ligA"
                     /locus_tag="ACG9CY_001789"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS4938294.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9941..10246
                     /gene="gatC"
                     /locus_tag="ACG9CY_001790"
     CDS             9941..10246
                     /gene="gatC"
                     /locus_tag="ACG9CY_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS4938295.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10246..11718
                     /gene="gatA"
                     /locus_tag="ACG9CY_001791"
     CDS             10246..11718
                     /gene="gatA"
                     /locus_tag="ACG9CY_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS4938296.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11715..13145
                     /gene="gatB"
                     /locus_tag="ACG9CY_001792"
     CDS             11715..13145
                     /gene="gatB"
                     /locus_tag="ACG9CY_001792"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS4938297.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13155..14210
                     /locus_tag="ACG9CY_001793"
     CDS             13155..14210
                     /locus_tag="ACG9CY_001793"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4938298.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14321..15694
                     /gene="rlmD"
                     /locus_tag="ACG9CY_001794"
     CDS             14321..15694
                     /gene="rlmD"
                     /locus_tag="ACG9CY_001794"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4938299.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15697..15825
                     /locus_tag="ACG9CY_001795"
     CDS             15697..15825
                     /locus_tag="ACG9CY_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4938300.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5397 a         2902 c         3481 g         4103 t
ORIGIN      
        1 gttttttatt tttttttgac tgatttataa tttttatgaa cgtttttgac tgatttatat
       61 tgcgagtaga aaaaaaatat gctattattt gtattgaggt gatcgtgaaa atgcttacag
      121 aggaacgtag acaaaaaatc ctagacttgc ttgaacagca gaaaatcgtg aaatcacaag
      181 atcttgtaaa cctttttaat gcttctgaat caacgataag acgtgattta caagagctgg
      241 agaatagtgg gtttctagaa agagttcatg gcggagctaa gaaagagcaa ttattgaatt
      301 ttgagcagaa tatgagtgaa aaatcactca aaaacgttca cgaaaaacag caaatcgcca
      361 aatcggcagc tgaaaccatt aatgacgaag atgtcattta tttagacgct ggctctacta
      421 cgttagaaat gattcctttt ctgcaaggaa aacagatcac agtagtgaca aattccgttc
      481 aacatgcggc aaatttagtt gatttggagc ttgctaccat tattttaggt ggaacgataa
      541 agctggcaac taatgctgtt ttaggatcta atgcgataga acaactaaaa aattttcatt
      601 ttaataaagc atttatggga atgaacggtg cccatcttga acaaggattc acgactccgg
      661 acccagaaga ggcagctgtt aaacgtttag ccatgaaaaa ttccgaagag acatttgtat
      721 tgattgatca taccaaattc aaccagttgt ccttcacctc tgttgcacct ttggaagcag
      781 ccacgatcat aaccgagcgt tgtccattag aattcgttaa tgaattccgt gaaaagacaa
      841 caattaagga ggcaatacaa tgatttacac agtaacactt aatccatcaa ttgattatat
      901 cgtacgcgta gatgggttaa aactaggcga tttgaaccga atgaaggaag acttcaagtt
      961 acctggagga aaagggatca atgtttcccg tattttgaaa cggatcgatt ctcaatctac
     1021 agcactaggg tttttaggag ggttcactgg cgaatttatt tcgaagtggc ttgaaaaaga
     1081 acaaatccag tctttgttca caaccgtaaa agaagataca agaatcaata tcaaactaaa
     1141 atcagatact gaaactgaaa ttaacggttt gggcccatct atttcagatg aagaaatcga
     1201 tgagttgaag caagcattag gaacagtagg acaaggagat atcgtcgtat tgtcaggaag
     1261 cgcgccggct agcctgcgaa aaggatttta tcaagaactg attgatatca ttcgagaaaa
     1321 aggtgcagat ttcgtgattg atacaacagg tgatgaccta aaaaatgcac ttgttaagaa
     1381 accattgttg atcaaaccaa acaatcacga attagctgag ctttacaata caaccttcca
     1441 atctgtagaa gaaatcattc cgtatggtaa aaaattgcta gctgaagggg cacagaatgt
     1501 gctgatctca atggctggag acggtgcttt gctcttcacg gaacaaggaa cttataaaac
     1561 gaatgtatta gttcgcccat tgaaaaattc agtaggtgca ggggattcaa tgattgccgg
     1621 ctttattggg gcttacagcc gtacgaaaga tccaatcgaa gcctttaaat ggggagttgc
     1681 ttgcggaagc gcaacagctt tttctgatga cttggcagct cgcgaattga ttgatgagtt
     1741 gctgccagaa gttgaaatca caaaaattga ttagtttcaa ggaggataat catgaatatc
     1801 aaggatttaa tggtcaaaga agcgatgatc atggatttgc aagcgactga taaaaaaggc
     1861 gcaatcgatg aaatggtcca aaaaatgttt gatgccggcc ggatttccga tatcgaaaca
     1921 tataaagaag gaattctagc ccgcgaagca caaacttcta caggtcttgg tgatggaatc
     1981 gccatgcctc acgcgaaaaa cagtgcagtt aaagaagcaa cagttttatt cgcaaaaagc
     2041 caaaaaggag tagattatga agccttagac ggacagccaa cttatctgtt tttcatgatc
     2101 gcagctccag aaggcgcaaa cgacacacat ttgcaagcat tagcagcact atctcgattg
     2161 ctgattgatg cagatttcgt aggaaaatta aaagaagcaa atacaccaga agaagtccaa
     2221 gacttgttcc aaacagctga agagcaaaaa gaagcagaag aaaaagcaga acaagaaaca
     2281 caagctgcag aagcttctgc tcatccagaa agaaaattcg ttgttgccgt tacagcttgt
     2341 ccaacaggga tcgctcacac ttacatggca gaagatgctc tgaagaaaaa agcgaaagaa
     2401 atgggtgtgg agatcaaagt cgaaacgaac ggttcagaag gaatcaaaaa ccgattgact
     2461 gctgaagaca tcgcccgtgc agaaggtgta atcgttgcag cagacaaaaa agtagagatg
     2521 aatcgctttg acggaaaaga actgatcaat cgtcctgtca gtgatggtat ccgtaaacca
     2581 gaagagctga tcaatctagc ccttagtggt actgcaccag tattccatgg tgatggaaaa
     2641 gaaagcagtt cagaagaagg aaatgcggat ggtactatcg gacaaagaat ttacaaagat
     2701 ttgatgaacg gcgtatcaca catgttacct ttcgttattg gtggaggaat cgctattgcg
     2761 ctttctttca tgatcgacca atttattggc gtgccacatg atcaattatc aaatctaggg
     2821 aattacaatc aagctgctag ctggttcaac caaatcggac aagctgcatt tggtttcatg
     2881 ctgccggtat tagcaggatt tattgcttca agtatcggtg accgccctgg tttgatcgtt
     2941 ggatttgctg ctggtgcatt agctaataca ggcggtgcag gattcttagg tgcactgatt
     3001 ggtggattct tagctggtta tgtcatcgta tttttacgta aattattcaa gaacttacca
     3061 aaatcattgg atggaattaa aacgatcctc ttttatccag tgtttggctt actgattact
     3121 ggtttcttga tgttgctggt caatgtccct atgaaagcaa tcaatgatgc attgaacggt
     3181 ttcttgacaa gtatgagtgg ttcgaatgct gctttacttg gtgcattatt agctggaatg
     3241 atggcggcag atttaggcgg tccaatcaac aaagccgctt atgtgtttgg tacagcaact
     3301 ttagctacga ctgttgcaac tggcggaagt gtcgtgatgg cttctgttat ggctggagga
     3361 atggtacctc cattggcaat tttcgttgct acacgtttgt ttaagaataa attcagcaaa
     3421 acagatcaag atgctggttt gacgaacatc gtgatgggac tatcgtttgt cacggaagga
     3481 tcgattccat ttgcagcagc tgacccgatt cgtgccattc caagttttat tattggttct
     3541 gctttaactg gcggattagt cggagctttc gggatcaaat tactggctcc tcatggcggg
     3601 attttcgttg tattcttact gagtcacccg gtcatgtatt tggtgtttat tgctatcgga
     3661 gcagtcgtat ctggtgtgat ttatggttta ttgagaaaag ctcctgcagc agcagaagca
     3721 tagaaataaa aaagaagagg ctggtttttc ccagtctctt ctttttttat agaatcatat
     3781 agaatcaagt agtgtagatg aatagttgtt gtaaaatagt ttttccatgt tcgttaaaat
     3841 gattgcttca aatttgtgat agaagattta gcctcgattt cctgcttgat tcttggcaag
     3901 tttgcttcat cagtcaagat aaccaaaata tctcctacat tcatgatcgt atcaccgtga
     3961 gtcaagattt cctgatttcc tctgcgtata gagattagca gcatattttt aggccaattg
     4021 aagtctcgta ccattaatcc gtctaaagaa ctctctgccg ttactggaaa atcaaaagtt
     4081 gtccttgcac cttttagtga ggcggtctga gtatttccaa gtagacgttc caataatgat
     4141 tcgtaaattg gattaccgcc gagaaggtca ttgacgatat aagcaacaag agcagccaat
     4201 cctaaaggca tcagatgatt aagagagcca accatttctg tcactaaaat aattgctgtc
     4261 agcggtgctt ttccaatcgc tgtgaaataa ccagccatgg caataacgac aaaatccttg
     4321 atgtagattg gatccatccc gaaatcaacc ataatgtttc cataaaagct accgattaaa
     4381 gcccccaatg tcaatatcgg aaggaaaatc ccccctggaa gaccaccacc gtaagaaatc
     4441 atggaaaaaa cgaatcgaag aacaaacaaa ccgataatca taatgattgg caaggaagca
     4501 tgtccaaaag ctaaaacgat ttgattgcct ccgcctagag tactaggcca taaataacca
     4561 attggcaaaa tcagcaagaa aggaacgaac ccgtgaaaat aagctggtaa aggcaatcgg
     4621 ttgtaaaatt ttggcaaagc cagcaagacc ttctgataaa taaaacctaa gatgcctaga
     4681 agtactccta agatcactaa ggaaccataa tatttcaacg gcaaagaagg aaggtcacct
     4741 agatataaaa caggtctcaa gccgaaaaag taaagagaga cgaagttagc agtgattgct
     4801 gaagcaaatg atgtcagcca aacaagtgga gaaaaggagt gatggatttc ttctaaaaca
     4861 aagagtaatc ctgcgatagg agcattgaat gctgctgcaa gtccgctact tgcaccgcta
     4921 gaaattaaaa tcttttcttc tgaagatgga ctccgaaaca attgtccagc tccttgtcca
     4981 atggatgcgc caagctgtat agaaggtcct tctctaccta gaaaaaggcc cgcaccaact
     5041 gaaagtacac cgccaatgaa ctttttccat aaaacagaga accagttcat tgataattcc
     5101 ccgcgaagct gtccctctac ttgtggaatc ccgcttccct tgatatccgg ttcatttttg
     5161 accagataac ccacgaaaaa tgctgcaagg atgcagcaaa tagctaggcc aattaagtaa
     5221 atcggctgtt cccgactcat ttcatagata cttcgaacta aatcgcttag cttttcgata
     5281 gataatcgaa acaggctaac gacgattcct gtcagcagtc caacaaatat tccttttaga
     5341 ataaaatata tctgagtctt atctaatttc ttgatttttc cttttttttc cattcaaacg
     5401 cctcgctttc ttttaattta gcgcgaaatc aacgaattga caaggaaaaa tccataaaaa
     5461 gaaacaaatg attttgaact agctagttta atgaaagcca agaaaatgct ataattaaca
     5521 agattgataa aagaaaggat gttgttaatg tcatccaaag ctgaattatt aaacgggatg
     5581 aacccccgtc agaaagaagc tgttcttcac actgacggac cattactgct aatggcaggt
     5641 gcaggaagtg gaaagacgag agtgctgaca caccgcattg cctatttgat tgaggaaaaa
     5701 gaagtcaatc cttggaacat cctagcgatt acttttacca ataaggctgc aaaagaaatg
     5761 aaagaacgtg tcaatgcgat tctcgcatct ggaggcgaag atgtctgggt atcgactttt
     5821 cactcgatgt gtgtgcgtat tttaagaaga gatgttgatt ttatcgggta taaccggaac
     5881 tttacaatca tcgattcttc tgaacagctg accttgatga aacggatact aaaagaactg
     5941 aacattgatc cgaaaaaata tgatcctcga agtatattag gaacaatctc tcaagcaaaa
     6001 aacagcctgc agacccctca ggattttgca aaaatgcaag gcagttatta cgaagagatt
     6061 gcggcaaaat gttatgctgc ttaccaaaaa gagcttcaat ataatcaatg tatggacttt
     6121 gacgacctga tcatgaatac gattcgatta tttgaagaac atcctgattc actcacatat
     6181 tatcaaaaca aattccatta tattcatgta gatgagtacc aagatacaaa tcacgcacag
     6241 tatacgctag tcaatttatt ggcaggtaga tttaggaacc tgtgcgtcgt gggagatgca
     6301 gatcaaagta tttacggctg gcgcggtgca gatatgcaga acatcttgga ttttgaaaaa
     6361 gattatccag atgctgcagt aattttgcta gaacaaaact atcgctccac gaaaaacatc
     6421 cttagcgcag caaaccaagt cattgaaaac aatagcaatc gaaaaccaaa aaacttgtgg
     6481 acagaaaaca aagaaggaaa caagatcact tactaccgtg cagataatga acgagacgaa
     6541 acccgtttta tcgtagatcg gatgcaagaa gagattcgaa gcaatcatcg gaattacggc
     6601 gactttgcca tcttgtaccg gacgaacgca caatctcgtg taatggaaga aactctgttg
     6661 aaagcaaata ttccttataa aatggttgga gggcacaagt tctacgatcg taaagaaatc
     6721 aaagatattt tagcgtattt gaatgtgctg gcaaatcctc aagattcaat cagttttgag
     6781 cgtatcgtta attcgcccaa acgaggcatc ggaccaggat ctatcgaaaa actacgcagt
     6841 tttgcttctc ttcatgaatg gccactgctt gaagcagcgc aaaatgtgga tctggcaaat
     6901 atcggtggaa aagcaggaca gcaattaggt gcatttggag agatgatcca agaagtcact
     6961 cagatgatcc catacttgac agtgacagaa ttgacgaaag aggttttgga tcgtagcgga
     7021 tatctagaag acttaaaaat ccaaaataca ttagaagcac aagcacgcat cgaaaacttg
     7081 gaggaatttt taacggtcac acaagaattc gacaaacaat tcgaacagca aaatgaagaa
     7141 gacgctgatg caccagaaga aaaactaacg gtcttcttaa atgatctggc acttgtttcc
     7201 gatattgaca atttagaaga agatgcttcc caagtgacat tgatgacttt gcatgcagcc
     7261 aaaggattag aattcccagt tgtattcctg attggtctag aagagggggt ttttccttta
     7321 tctcgagcat tgatggaaga aagcgaactg gaagaagaac gccgtctcgc atatgtgggg
     7381 atcacgcgag cagaagaagc gttgtattta acaaatgctt tctccagaac attatatgga
     7441 cggacgcaat ataaccgacc ttctcgtttt gtagaagaaa tcgatcagga gttactggaa
     7501 atcgaaggca tgcgtcctac gcctaaaaaa acacctgtat tcgctaaaaa aacagcatat
     7561 agctataaac agccagagac agcggttgtt ccctctaaaa gtgctactgg aggggaaaat
     7621 aacagctgga aacctggaga taaagtcaaa cacaaaaaat ggggtctagg aacagtcgtc
     7681 cgagtaagcg ggacgtcgaa ggatctagaa ctagatgtgg ctttcccaag tcaaggagta
     7741 aaacgtcttt tggctgcctt tgcgccaatt gaaaaagctt agtagtggag ggtaaatgat
     7801 ggaacatgaa atgacgattg acgaagctgc taaacgagca gaagaacttc gtacaagact
     7861 gaaccaatgg tctcgcgaat actacgtaga agataaacca acagtggaag actatgttta
     7921 tgataaagaa tacgcagaat tagtagcaat tgaggaacaa tatcctgatt tgatcacgtc
     7981 tgattctccc actcaacgag taggcggaaa agtattagaa ggttttgaaa aagtcactca
     8041 tgatattccg ctttacagtt tgaacgacgt tttcagcaaa gaagagctga tagcttttga
     8101 tcaacgtgta caaaaagcag tgggacgtgt agttgactat tgctgtgaac taaaaataga
     8161 tggactttcc gtttctttgc gttatgaaga tggaaatttc gtgagaggtg cgactcgcgg
     8221 ggatggaact gtaggagaaa acatcactga aaacctaaaa actgtccggt cggtcccaat
     8281 caaattaaaa gaaccaatga acatcgaagt aagaggcgaa tgtttcatgc caaaacggtc
     8341 atttgtccaa ttgaaccaag acagagaagc agagggaaaa gatatctttg cgaatcctcg
     8401 gaacgccgca gctggttctt tgcgtcagtt agattctaaa atcacagcga aaagaaatct
     8461 ggacactttt ttatacacag tagctgattt cggtcctatg caagcaaaaa cccagtatga
     8521 tgcgctagaa gagctagaaa aaattggatt ccacacgaat cgggaaaaac gtctatgtca
     8581 ttcgattgat gaagtgtggt catacattga agaatatcat gacaaacgtg tggatttgcc
     8641 ctatgaaatc gatggaatcg tcatcaaagt gaatgaattc tcgctacaag atcaacttgg
     8701 ttttactgtc aaagcaccga gatgggcagc agcttacaag tttccgccag aagaagttga
     8761 aacgctaatc gaaaacatcg aatggacagt cggtcgaaca ggggtagtaa cgccaacagc
     8821 tatcatgaca ccagtccgag tagcaggaac gacagtaagc cgtgccagcc tgcataacgg
     8881 tgattatatt aagttgaagg atatccgctt gaaagataca gttctgattt ataaagcggg
     8941 agacatcatc ccagaagttt cgcaagtcgt gttagataaa cgacctaaag acagcgaaga
     9001 ataccaattg cctacacatt gtccagtttg tggaagtgaa cttgtccatt tggatgaaga
     9061 agtagcctta cggtgcatca atcctaaatg ccctgctcaa atgaaagaag gtttgaacca
     9121 ctttgtttca agaaatgcga tgaatatcga tgggttgggg ccgcgcgtac tagaacagat
     9181 gtatgataaa aaactagtag cagatgttgc tgatctttac aaattgacag aagaagagtt
     9241 attgacgttg gacaaaataa aagaaaaatc agcaaataat attttgacag caatcgacaa
     9301 cagcaaagat aattcggtcg agcggttgat tttcgggtta ggaatccgac atgtcggagc
     9361 gaaagcagca aaaattttag cagaacattt cggtgatcta gaaacattga gtaaaagtga
     9421 ctatgagtcg attattgcgc ttgatacgat cggtgatatc attgccgaca gtgtagtcac
     9481 ttatttcagt aacgaagaag tccatgaatt gatgaatgaa ttaaaacaag caggcgtcaa
     9541 tttcgaatac aaagggcttc gaaatgccca attgcaagaa gtcgagtctc cttttaaaga
     9601 aaaaacagta gtccttactg gtaaactaac acgatttact cgtgaagaag caaaagagac
     9661 gatcgaaaat cttggcggga aagtgactgg tagcgtatcg aaaaaaacgg atatcgtggt
     9721 agcaggagaa gatgctggca gcaagctgac caaagcacaa gaattaggaa tcgaagtttg
     9781 gacagaagat caaatggcag atgcattggc taaaagtcgc tcagtagaag aatgaaaaat
     9841 gagttttttc agacaaattt caataaacaa tttcagacac ggcactcttg tgttaaaata
     9901 gagagcaaag tttttaactc gtttagaaag aaggggtagt atggcgatta gtgaagaaca
     9961 agtgaaacac gttgccaaat tagcaaaact tgcgttcgca gatgaagaac ttgcaagttt
    10021 taccgaccaa ttaggtaaaa tcatcgatat ggttgaaatg ctggaagaag tagatacaga
    10081 aggtgttcca tttacgtcga atgtggttga aacagtaaac gtgatgcgac aagatcgggc
    10141 gcaagctgga tggaaccgcg atgaattatt gaaaaatgtg ccggaaacag aagacggctt
    10201 tatcaaagta cccgcaatca tcgataatgg ggaggctggc gcataatgac agaattacac
    10261 aatcgttctc tagaagaact gcatcatatg cttgtatcga aagaaatcac tgtacaagat
    10321 ttgacaaaag caacatttga acgaatcaaa gaaacagagc ctgaggtcga agcattcatt
    10381 acattgaacg aagaaaaagc attggaacag gcaaaagctt tagatgaaaa agggattgat
    10441 gaaagcaacg tccttgctgg tattccaatt ggtatcaagg ataatatcgt aacaaaagat
    10501 atcttgacaa ccgctgcatc taagatcctt tacaattttg aaccaattta tgatgcaacg
    10561 gtcatggata aaatctatca agcagatatg atccctgttg gtaagctaaa catggatgaa
    10621 tttgctatgg gtggaagtac agaaacttct tatttcaaga aaacaaaaaa tgcttgggac
    10681 ccaacaaaag tacctggtgg ctcttctggt ggttctgcag cagcagttgc agctggacaa
    10741 attccagttt ctttaggtag tgatactggg ggaagtatcc gccaaccagc agcttttaac
    10801 ggcatcgtag ggatgaaacc tacttatggc cgtgtctcac gttttggttt gattgccttt
    10861 gcttcatctc ttgaccaaat cggaccaatg acccgtacgg tgaaagataa tgcattagca
    10921 ttgacagcta tcagcggtta tgacgaaaaa gacggtactt cttcaggcgt ttcagttcct
    10981 gattttgcag agggactgac aggcgacatc aaaggaatga agattgcttt acctaaagaa
    11041 tacttaggcg aaggtgtggc ccctggcgta aaagaagctg tattgaaagc agcagaaaca
    11101 ttccgttcat taggtgctgt tgtggaagaa gtcagcttgc ctcattcaaa atacggtgtg
    11161 gctgtttatt atatcattgc ctcatcagaa gcaagttcca acttgcaacg attcgatggt
    11221 atccgctacg gttatcgctc agaaaatgtg aaatcattag atgatgtata tgtcaactca
    11281 cgttcagaag gattcggtga agaagtaaaa cgccgtatca tgttaggtac attctcctta
    11341 agtgctggtt attatgacgc ttacttcaaa aaagcgggac aagtccgtac attgatcaaa
    11401 cgagattttg aaaaagtctt tgcagattat gacttgatca tcgggccatc ttcaccaaca
    11461 gttgcattcg gaatcggtga gaacattaac gatccaatca caatgtacat gagtgacatc
    11521 ttaacgatcc ctgtcaactt ggctggactt ccaggtatgt ctgtaccggc aggtttatca
    11581 gaaggattac cagttggatt gcagatcatc ggtaattatt ttgatgaaaa aacgatgtat
    11641 caagcggcat atgcgtttga acaagcaaca gaattccata aacaacaacc agctatttta
    11701 ggagggaaag acgaatgaat tttgaaacag tcattggact agaagtccac gtcgagttaa
    11761 aaaccaactc aaaaattttc tctccagcgc cagctcattt tggtgcagaa ccaaacagca
    11821 atacaaacgt gatcgactgg ggttatccag gcgtgctgcc agtaatgaat aaagctgcgc
    11881 tggaattcgg gatgaaagca gctttggcat taaactgtga aatctctaaa gatactcatt
    11941 ttgaccggaa aaattacttt tacccagata atccaaaagc atatcaaatt tcccaatttg
    12001 atcaaccaat cggtcatgac ggatggatcg aaatcgaagt agaaggtaaa aagaaaaaaa
    12061 tccggatcga acgtgtccat ttagaagaag atgctggaaa aaacatgcat ggtattggcg
    12121 gttattctta cgttgatttg aaccgacaag gcacgccttt gatcgaaatc gtttctgaag
    12181 ctgatatgcg ttcaccagaa gaagcatacg cttatctaga agcattgcga tcaattatcc
    12241 aatttacaga agtcagcgat gtgaaaatgg aagaaggctc tatgcgctgt gatgccaata
    12301 tttctcttcg cccttatgga caagaagagt tcggtacgaa agcagaattg aaaaacttga
    12361 actctttatc tttcgtgaaa aaaggtttag cttttgaaga aaaacgtcaa gcaaaagttc
    12421 ttctttcagg aggagaaatc caacaagaaa cacgccgttt tgacgaaaca acgaataaaa
    12481 cagttttgat gcgtgtcaaa gaagggtcta gtgattatcg ttacttccca gagcctgata
    12541 tcccgaaatt cgtgatcgat gacgaatgga ttcaaaaagt aaaagctagt ttgcctgaga
    12601 tgccagcttc tcgtcgcgaa cgctatatcc gtgagtttgg acttccagag tatgacgcaa
    12661 tggtattgac acttacaaaa gaaatgtctg atttttttga agctgttttg gaaaatggtg
    12721 cagatgccaa gcaagcatct aactggttga tgggtgaagt ttctgcttac ttgaacagtg
    12781 agaaactgga attagctgag acaaaattga cacctgagaa cttagcagga atgattcgtt
    12841 tgatcgccga tggaacgatc agctcaaaaa tcgccaaaaa agtcttccgt gaattgatca
    12901 caaatggcgg agaagcccaa gaagtagttg aaaaaaatgg ttgggtgcaa ttatctgatc
    12961 cgaacaaact actgccaatc attaatgaaa ttttggataa caaccaacaa tctgtcgatg
    13021 atttcaaaaa tggaaaagat cgtgcggttg gcttcctagt cggtcaaatc atgaaagcaa
    13081 ctaaaggaca agcaaatcct ggtgtcgtga ataagctact taaagatgaa ttagcgaaac
    13141 gatagaggct gaaaatgaaa aaagcacgcg taatttataa ccccacctcg ggaaaagaat
    13201 tattgaaaaa aaatttggcg gatatcctac aggcattaga agaagcagga tttgaagcca
    13261 gcgcctatgc aactacacca gaaccagatt cagccaagaa tgaagcatgt agagtcgcag
    13321 aactaggttt tgatctagta gtggcagctg gtggcgatgg cacaatcaac gaagtggtta
    13381 atggaatcgc tccgcttgaa caccgtccta aaatggccat catcccagct ggtacaacga
    13441 acgactatgc tcgagcatta aaaattccta gagataatat taaagcagca gcagaagtga
    13501 taaaaaaaca ccaaaccgta aaaatggaca tcggcctttc tgataacagt tacttcatca
    13561 atatcgctgc aggtggctat ttgacagaat tgacttatga agttccatca gaactaaaga
    13621 gtatttttgg ttatcttgct tatcttgcga aaggagcaga gatgctccct cgagtaaagc
    13681 caatcaaaat gcgcttgaag tatgatgaag gggaatatat cggaaatgct tcgatgtttt
    13741 tcctagggct gacgaactct gttggaggat tcgaacagat cgcgccagat gccaaattgg
    13801 atgatgggaa gttttcttta atcattgtga agacagcaaa tatctttgag atcttgcata
    13861 ttgcagcact catgctgaat ggcggtcgac atgtcgatga tccaagaata atctatacca
    13921 aaacaagcag tttgttcgtt gaaacagaag atgatatcaa tcgaccagta atgatcaact
    13981 tagatggtga atacggcggg gaagcaccga tgcatttcca aaatcttcat caacacattg
    14041 agttctttgc caataccgat cagattcctg atgaagcaat cacaggtaca gatgaagaag
    14101 aattagaggt tgtcagcaaa gaattcgtaa aagaagtcga acgtttaacg gatgaagata
    14161 tcgacggtga cggaaaaata gcagacgaaa cacctaatca aaagaattaa tcaaaagccg
    14221 gtgctgaacg tagtaaggtc agtgccggtt ttagttgtgt aattagctga tttatagtag
    14281 agttcagtag gattgaaaaa taggcttaaa ggagcaaaat atgacagaac agcaaatagt
    14341 aaagaaaaac gaacgatata cagtcgaaat cgtcgacttg agttatgaag gaatgggggt
    14401 agcaaaaatc gatggttatc cattatttat cgaaaatgca ctccctggag aagaagtaga
    14461 aatactagta gtaaaagcag gcaataagtt tggatatgga aaagtcgaaa catttattac
    14521 acaatcacct gatcgtcagc ctgtagaaaa caatgcatta cttcgcatag gtatcgcacc
    14581 tttagcccat ttgaagtatg aacaacagct tgtcttcaaa caaaaacaag tagagcatgt
    14641 aatggcgaag attgctaaga tgccagaagt tcctgtactt ccaacggtcg gaatggcaga
    14701 accattcggc taccgtaata aagcacagat ccctgttcgc cgtatcgatg gacagttagc
    14761 caccggtttt tacaaaaaga atagccatga tctagtccct atcgaagatt tttatatcca
    14821 agatcctgtc atcgatcaag caatcaaagt agtcagagat atcttgcaac ggttccaagt
    14881 acgaggatat aatgaagaaa aaaatgaagg acagcttcgt cacatcatta tccgtcgtgg
    14941 tcattattcc catgaaatga tggtcgtttt agttactcgt aaagaaaaat tctttaaatc
    15001 caaggaaatt gctgaagcta ttcatgaaga attgccagaa gtggtttcgg tcatccaaaa
    15061 tatcaatgaa gaaaaaacaa atgtgatttt aggagacaaa gaaaaagttt tatatggcag
    15121 agactatatc gaagatcagc tattaggaaa aacctatcga atctcagcaa agtcatttta
    15181 tcaagtaaat actcaacaag cagagtatct gtaccaaaca gccatcgagt ttgccgagtt
    15241 agataaaaaa gacattgtga tcgacgctta ttcaggaata ggtacgattg gcttgtcttt
    15301 agcagacaga gtggataagg tgtatgggat ggaagttatt ccagaagcaa ttgaagatgc
    15361 acagttcaat gctttaaata ataaaatcga aaatgttcat tatgaagtcg gaaaagcaga
    15421 aaaagttatg aaaaaatggc aagaaaaagg gatcaaacca tctgtgatcg tagttgaccc
    15481 tccacgaaaa ggattagatc atcattttat tgaatcagca atcgaaatgt caccagaacg
    15541 aatcgtctat atctcatgta atccggcaac ttttgcaaga gatgctaagt tatttgcaga
    15601 agcaggttat gccgtgaaga aagttcagcc tgtagatctt ttcccgcaaa cccatcatat
    15661 cgaattagtc gcttcattcg ttaaaggaga ataaaaatgg accaaaaaga aaaaatgaaa
    15721 gaattgatca aaaagaaaca aggtggaggc cgatccaaac aaatggaaac acctaagaat
    15781 gatcgtaaaa acatgagaaa aggccctaaa atctttaata aatagacgtg ctaaagaaag
    15841 actaagaagt gttaactttt gttattgttt cttagtcttt tta
//
LOCUS       DAZULG010000063        15138 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000063 DAZULG010000000
VERSION     DAZULG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.065"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACG9CY_001796"
     CDS             4..2376
                     /locus_tag="ACG9CY_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS4938301.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACG9CY_001797"
     CDS             2437..3897
                     /locus_tag="ACG9CY_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS4938302.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACG9CY_001798"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACG9CY_001798"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4938303.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACG9CY_001799"
     CDS             6128..6277
                     /locus_tag="ACG9CY_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938304.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACG9CY_001800"
     CDS             6352..6945
                     /locus_tag="ACG9CY_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS4938305.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACG9CY_001801"
     CDS             6958..7857
                     /locus_tag="ACG9CY_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS4938306.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACG9CY_001802"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACG9CY_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4938307.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACG9CY_001803"
     CDS             8723..8983
                     /locus_tag="ACG9CY_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938308.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACG9CY_001804"
     CDS             9428..9634
                     /locus_tag="ACG9CY_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS4938309.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACG9CY_001805"
     CDS             9655..9885
                     /locus_tag="ACG9CY_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938310.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACG9CY_001806"
     CDS             9901..10338
                     /locus_tag="ACG9CY_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938311.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="ACG9CY_001807"
     CDS             10331..11038
                     /locus_tag="ACG9CY_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938312.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11202..11402
                     /locus_tag="ACG9CY_001808"
     CDS             11202..11402
                     /locus_tag="ACG9CY_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938313.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11418..11597
                     /locus_tag="ACG9CY_001809"
     CDS             11418..11597
                     /locus_tag="ACG9CY_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938314.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="ACG9CY_001810"
     CDS             11922..12185
                     /locus_tag="ACG9CY_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFS4938315.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="ACG9CY_001811"
     CDS             12179..12529
                     /locus_tag="ACG9CY_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4938316.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="ACG9CY_001812"
     CDS             complement(12553..13167)
                     /locus_tag="ACG9CY_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4938317.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13481..13903
                     /locus_tag="ACG9CY_001813"
     CDS             13481..13903
                     /locus_tag="ACG9CY_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938318.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13913..14116
                     /locus_tag="ACG9CY_001814"
     CDS             13913..14116
                     /locus_tag="ACG9CY_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938319.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14327..14911
                     /locus_tag="ACG9CY_001815"
     CDS             14327..14911
                     /locus_tag="ACG9CY_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4938320.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5571 a         2451 c         3060 g         4056 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
    13501 atctttaggt attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc
    13561 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
    13621 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
    13681 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
    13741 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
    13801 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
    13861 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
    13921 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
    13981 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
    14041 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
    14101 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
    14161 ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca
    14221 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
    14281 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
    14341 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
    14401 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
    14461 agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
    14521 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
    14581 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
    14641 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
    14701 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
    14761 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
    14821 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
    14881 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
    14941 ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct actgataact
    15001 tattctcaaa tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa
    15061 aaagtccgta ctctaatttg cagtctctca ctcttttaga gtgagagttt ttattagatt
    15121 ttcaaattaa ttagtaaa
//
LOCUS       DAZULG010000064        15068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000064 DAZULG010000000
VERSION     DAZULG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.066"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..222
                     /locus_tag="ACG9CY_001816"
     CDS             <1..222
                     /locus_tag="ACG9CY_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFS4938321.1"
                     /translation="TEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIR
                     GLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            403..1362
                     /locus_tag="ACG9CY_001817"
     CDS             403..1362
                     /locus_tag="ACG9CY_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS4938322.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMRGKRKPNGHQEKRGKQQYQRSIHDRSDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            1684..1854
                     /locus_tag="ACG9CY_001818"
     CDS             1684..1854
                     /locus_tag="ACG9CY_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938323.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            1868..2470
                     /locus_tag="ACG9CY_001819"
     CDS             1868..2470
                     /locus_tag="ACG9CY_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4938324.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            2486..3163
                     /locus_tag="ACG9CY_001820"
                     /pseudo
     CDS             2486..3163
                     /locus_tag="ACG9CY_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3474..3971
                     /locus_tag="ACG9CY_001821"
     CDS             3474..3971
                     /locus_tag="ACG9CY_001821"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFS4938325.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            3972..4388
                     /locus_tag="ACG9CY_001822"
     CDS             3972..4388
                     /locus_tag="ACG9CY_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFS4938326.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            4408..5952
                     /locus_tag="ACG9CY_001823"
     CDS             4408..5952
                     /locus_tag="ACG9CY_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4938327.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            6095..6319
                     /locus_tag="ACG9CY_001824"
     CDS             6095..6319
                     /locus_tag="ACG9CY_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938328.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            6334..7203
                     /locus_tag="ACG9CY_001825"
     CDS             6334..7203
                     /locus_tag="ACG9CY_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4938329.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            7184..7918
                     /locus_tag="ACG9CY_001826"
     CDS             7184..7918
                     /locus_tag="ACG9CY_001826"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4938330.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7951..8859
                     /locus_tag="ACG9CY_001827"
     CDS             7951..8859
                     /locus_tag="ACG9CY_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS4938331.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            8856..9398
                     /gene="sat4"
                     /locus_tag="ACG9CY_001828"
     CDS             8856..9398
                     /gene="sat4"
                     /locus_tag="ACG9CY_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS4938332.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            9491..10285
                     /locus_tag="ACG9CY_001829"
                     /note="aph(3')-IIIa"
     CDS             9491..10285
                     /locus_tag="ACG9CY_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4938333.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            10561..10692
                     /locus_tag="ACG9CY_001830"
                     /pseudo
     CDS             10561..10692
                     /locus_tag="ACG9CY_001830"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            10768..11261
                     /locus_tag="ACG9CY_001831"
                     /pseudo
     CDS             10768..11261
                     /locus_tag="ACG9CY_001831"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            11431..12327
                     /locus_tag="ACG9CY_001832"
     CDS             11431..12327
                     /locus_tag="ACG9CY_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4938334.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            12426..12635
                     /locus_tag="ACG9CY_001833"
     CDS             12426..12635
                     /locus_tag="ACG9CY_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4938335.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            12652..12924
                     /locus_tag="ACG9CY_001834"
     CDS             12652..12924
                     /locus_tag="ACG9CY_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4938336.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12926..13789
                     /locus_tag="ACG9CY_001835"
     CDS             12926..13789
                     /locus_tag="ACG9CY_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4938337.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(14052..14789)
                     /gene="erm(B)"
                     /locus_tag="ACG9CY_001836"
     CDS             complement(14052..14789)
                     /gene="erm(B)"
                     /locus_tag="ACG9CY_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4938338.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14914..14997)
                     /locus_tag="ACG9CY_001837"
     CDS             complement(14914..14997)
                     /locus_tag="ACG9CY_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS4938339.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5344 a         2382 c         3087 g         4255 t
ORIGIN      
        1 acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca tcgtccagta
       61 aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat taaaggcatg
      121 gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt cgggttttcg
      181 gtctgtactg aaatcaaggt attattggga atcccagctt aaatcataga taccgtaagg
      241 gattttattc tttatttaaa actttgcaac agaaccaccc gcactatgcc attacattta
      301 tatctacgcg aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg gtacactcaa
      361 attgtatttc cgacgaaaga aaacaaagga gtgtaaccat aaatgaccta caaacatctt
      421 accatagacg aactgacaat gatagaatca tattatcttc aacataataa gccggttgaa
      481 atcgctaacc gaatgggacg tgctatacaa actatttata atgtagtcaa taagttcaag
      541 caaggcaaga ctgctcttga ttattggcac cagtataaag aaaataagaa aaaatgtggt
      601 agaaaagtca ttcaattacc tgctcatgaa gtagattaca ttaaagagaa agtcactctt
      661 ggttggacgc ctgacgtcat tatcgggcga aaagaaaggt ctgtttcatg cggtatgaga
      721 acactttatc gtttattttc taaaggaata tttgatattg acacactacc gatgagaggt
      781 aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac aacagtatca gcgctcaatc
      841 catgatcgat ctgataatta tcctgatttc aattctgagt ttggtcacct tgaaggtgat
      901 acgatcgttg gcattcatca taaaagtgcc gtcattactt tagttgaaag attatctaaa
      961 gtcattatca cgattaaacc caacggccgt aaggcattag atattgaaac tgcccttaat
     1021 caatggtttt ctcgcttccc taaaaacttc tttaaatcta ttacgtttga ctgtggaaaa
     1081 gaattttcta actggaaagc cattagtaac caacatgata ttgatatata ttttgcggac
     1141 cctggaacac cttctcaacg cccattaaac gagaattcta acgggattct gcgtcgtaat
     1201 ggactgccga aatcaatgga ttttagaaaa gtgaatcaga catttatttc cagtgtcagc
     1261 aatcaacgta atcatattcc aagaaaatca ttgaattaca gaacaccaat cgaaatattt
     1321 ttgagctatg tacaagaagc attttattct agcttaattt gacaaatcat actatgatac
     1381 gtgtttgttt ttctgtgctg tttagtgaat gattagcaga aatatagaga gtaagatttt
     1441 aattaattat tagggggaga aggagagagt agcccgaaaa cttttagttg gcttggactg
     1501 aacgaagtga gggaaaggct actaaaacgt cgaggggcag tgagagcgaa gcgaacactt
     1561 gatcttttaa gttgctatct ttttaaggtc aatagagtat acttatttgt cctaatatgg
     1621 ttttagcagt ataattgact tggtgaatag gtcatttaag ttgggcataa taggaggagt
     1681 aaaatgaaaa aatttattta tcgagtttta gaaaatgacg aagtggtggc tatttttaat
     1741 gagcaacaat atgcgcaaga ttttatcgct tacgaaaaga caatttctga taagcaattt
     1801 gaaattgaaa aagtagatat agctgattgg ttattgcaac cgagagaatt ttagaggttg
     1861 gttgaaaatg gctaaaattg gttatgcacg tgtcagtagc aaagaacaga acttagatag
     1921 acaactgaaa gcgttagagg gcgtttctaa ggtcttttca gacaaattaa gcggtcaatc
     1981 ggtcgaacgc ccacaattac aagctatgct taactatatt cgtgaagggg atatagttgt
     2041 tgttactgaa ttagatcgat taggacgaaa taataaagaa ttaacagaat tgatgaatca
     2101 aattcaaatt aagggggcaa ccctggaagt cttaaattta ccctcaatga atggtattga
     2161 agatgaaaat ttaagacggc tgattaataa tttagtgatt gaattgtata agtaccaagc
     2221 ggaatctgaa cgcaaacgaa ttaaagaacg ccaagcccaa ggaattgaaa ttgctaagaa
     2281 aaaaggaaaa ttcaaagggc gacaacctaa attcaaagaa aatgatccac gtttacaaca
     2341 cgctttcgat ttatttttga atggttgttc cgataaagaa gttgaagaac aaactggaat
     2401 taatcgccga acgtttagaa ggtatcgatc aagatacaac gtgacagtag atcaaagaaa
     2461 aaaacaatga aaagagggat agttaatgag tacggttatt ttagctgaaa aaccaagcca
     2521 ggcattagcc tacgcaagtg ctttaaaaca aagcaccaaa aaagacggtt attttgagat
     2581 caaagaccca ctatttacag atgaaacgtt tatcaccttt ggttttgggc atttagtcga
     2641 attagcagaa ccaggtcatt ataacgaaaa gtggcagaac tggaaacttg aatctttgcc
     2701 gatttttcct gatcgatacg attttgaagt tgcaaaagat aagggaaagc agtttaaaat
     2761 tgttgctgaa cttctcaaaa aggcaaatac aattattgtc gcaacagata gcgacagaga
     2821 aggtgaaaat atcgcctggt cgattatcca taaagcaaat gccttttcta aagataaaac
     2881 gtataaaaga ttatggatca atagcttaga aaaagatgtg atccgaagcg gttttcaaaa
     2941 tttgcaacca ggaatgaatt actatccctt ttatcaagaa gcgcaaacac gccaaattgc
     3001 cgattggttg atcggcatga acgcaagccc tttatatacc ttgaatttac aacaaaaagg
     3061 cgtacaaggt acattttcat taggacgtgt acaaacaccg actttatatc tcatttatca
     3121 acgccaggaa gcaatagaaa actttaaaaa agaacctttt ttcctgaata attcatagtt
     3181 ttactaatag tctttcctaa cattgattta atagtcttta aagtattctg cttcatcacc
     3241 cattaggtga tgaaaaaaga acccctttct gatttagcgg acaaatactt tgacctaatc
     3301 tcagaagata taaaagaaaa aataataaaa tatccgattt atactttgta taaagaacta
     3361 aagtagttag caaatcattt acagcaaatc actatgttcc acataccatt ttacatactt
     3421 accttatcca cgacacatgt tgagatagta tcgaagataa agaaagttgc tccatgttgt
     3481 ttttgcgaga tggggaaatc aaagccacat tgacaaccat gatgaataag cttgctttca
     3541 gtcataagct aatcctagaa ccacttttca agtcagttag ccaaatcgat gaagaaagcg
     3601 accgtgaaag aatggacgct attgataagc taatggagca actattggaa gaacgcaata
     3661 cccttattgc actgatgtcc aaaggtttcc tagaacctgc tctttttaat caggaacgaa
     3721 atgttttaga tagtgagatt aaaaatctta ctaccgaaaa aacaaacctg gtaacgaact
     3781 ctgcgagtgg ggttttgcga gcaaacgaga taaaggacct cattaattat gtgtcagcag
     3841 ataattttaa tggtgactac acggaagaac tatttgaaga atttgtagtg aacatcattg
     3901 taaattccag ggatgagctg acattcaatt tgaaatgcgg tctttccctg aaagaaaagg
     3961 tggtgagata aatggcatat attccatatg gatacaaaat tcaagatgga gtggttactg
     4021 tcgatgaaaa ggcagcaggt caagtaaagg tattctttga gaaatacata tcaggactat
     4081 cccttacagt ggctggcgaa caggcaggta ttgaaaagac acattctgtg atggggcgca
     4141 ttttgaaaaa cgtcaactac cttggaaatg atacgtatcc agcaattatt gataaagaga
     4201 tatttgataa agctgaagaa gttagagata aacgtgcaaa ggatttagga cgagtggtag
     4261 agcttgccgc tttcacctct ccccctccca aagaacgatt caaaatgaaa aaggcagata
     4321 ataagatgcc agtggatcct tttgaacgag cagaatactt atacagtctg atagaaagcg
     4381 aggaataaag tgacagagaa aaatataatg gttattcctg ctcgtaaaag agtaggaagt
     4441 acagccgcaa aagaaaagat aaagaaactt cgtgttgctg cctattgccg tgtttctaca
     4501 gaaacagaag aacaaaattc tagctatgag gttcaggtct cacactatac agagtttata
     4561 aagaaaaata atgaatggga gtttgctggc atctttgcag atgatggtat ctctggtacg
     4621 aacactaaaa agcgtgacga atttaatcgc atgattgcag agtgtatgga cggtaacatc
     4681 gacatggtta ttacaaaatc catcagccga tttgcgcgta acaccctaga ctgccttcaa
     4741 tacattagac agctcaagga taagaacata tcagtttatt ttgagaaaga gaacatcaac
     4801 accacggatg ccaaaggtga ggttttgctg actattatgg catctttggc acagcaggaa
     4861 agccagagcc tttcacaaaa cgttaagctt gggctacagt accgatacca acaaggaaag
     4921 gtgcaggtca accataagcg atttatgggc tacacgaaag atgaagatgg aaatttaact
     4981 attgtccccg aagaagctga gattatcaaa cgcatctacc gagaatacct tgagggtcag
     5041 agtctagtgg gcattggtcg agcccttgaa aaggatggta ttttaacagc agcgggaaaa
     5101 ccaagatggc gaccagaatc agttaagaag atacttcaaa acgaaaaata catcggagat
     5161 gcccttctgc aaaagactgt cacagtagat tttctgacca agaaacgagt gaaaaatgaa
     5221 gggcatgttc cccaatatta tgttgaaaat agccatgaag cgattattcc taaagagtta
     5281 tttttgcagg cgcaagaaga aattcatcgg agaagcaata tctacacagg agaaggcaag
     5341 aacaaaagaa tttacagtag caagtacgct ttaagtgcca tcaccttctg tggagattgt
     5401 ggcgatattt atagacgggt ctattggaat atccacggaa gaaaagaatt tgtctggcga
     5461 tgcgtgacta gaatcgagca aggtcctgaa gtttgtaaga accgaaccgt aaaagaagat
     5521 gaactttatg gtgcagtaat gactgcgatt aataagctac ttgcaggtgg gaacaatatg
     5581 ataaagacac tggaagagaa tattcatgct gtgattggcg aaaccacaga ataccaaatt
     5641 tcagagatta acaactcact agaggaaaaa caaaaagaac taatcaagct ggcgaacaag
     5701 ggtcaagact atgaccactt agcagatgag attgatgagc tgagagacaa gcgacagatc
     5761 cttttagtag aagatgcctc cctcagtggc gagaacgagc gaatcaatga gctgattgaa
     5821 tttatccgca agaacaaatt ccgcacctta gaatatgatg ataagcttgt aaggaagata
     5881 atccagagcg ttacagtcta tgaaggccac ttcgtcatag ccttcaaatc tggcatcgaa
     5941 atggaaatat gaaaaccaga agtaaatagc ccatgactct acagtagagt tgtgggtttt
     6001 cttttgcttg ctgattataa taatatttta ataactattg tgtttatcaa ccaaatggtt
     6061 tataatattc ttagcgtaaa cttggaggtg ctgtatgact acggcagaaa tgattaaaga
     6121 actgtgtgag caaatgaata taagtgtttc cgaacttgct agacgtattg gccagactcc
     6181 acagaatttc aataaaaaat tacaacgaga aacagtaacc ttggatgagt tgaaggccat
     6241 tgctgatgtg ctaggtgtca agtttgtgca ggcatttatt ttaccagatg gagaagaaat
     6301 aaaaatatct aacgaataaa ggaggatgtt tatatggtag gtaatgttat tcaaatagta
     6361 acagagaaat tatcatcttt gccttttata gaaggcatcg tattaggggg ctcacgtgca
     6421 agaggcaccc atacagaaaa ttctgatata gatatcggaa tctattacaa ttcagactca
     6481 tttgacctga cagcgattaa tcaaattgct acagagttgg atgatgagaa tagaaacaac
     6541 cttgttgtac ctcccggagc gtggggtgat tgggttaatg gcggtggatg gttagttata
     6601 aacgggtgtc atgttgactt gatattacgt gatataaaac gggtggaaca aataatcaaa
     6661 gatacggagc aaggaattgt tactgccaat tatcagaccg ggcatcccca tggttatata
     6721 agtgctatgt atcgtggaga attagcgatt agtaaaatac tatatgctaa gaacgaaagc
     6781 ttatgcgaat taaaaaagca ggcagaaatt taccctactg ctctaaagaa aagcttgatg
     6841 aactttttta tatttgaagc tgaattctct ttaatgtttg taaaagcaaa tgcgggggca
     6901 gaggataaat attatattgc aggccatgtt tttcgtatta tttcatgctt aaatcaagta
     6961 ctatttgcat gtaataatgc ttattgcatt aacgaaaaga aagctataaa actgcttgaa
     7021 acttttgaat ataaacctga aaaatatgcc gaaagagtta atcatatttt tgaagtactc
     7081 ggtctttctc tttttgaatg ctacgacatg accgagaaac tttataaaga agtgaatgaa
     7141 attgtatcgg agataaataa ctttttaaac gaggagagtt cagatgaaag aaaacaaata
     7201 tgatgataat atattttttc aaaaatacag tcaaatgagt cgctcgcaga aaggactggc
     7261 tggtgcggga gaatgggaga ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt
     7321 gcttgattta ggatgcggct atggatggca ctgtatatat gcgatggaaa acggtgcttc
     7381 ctctgtagta ggtgttgata tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca
     7441 ttttccacag attgaatatg aatgctgtgc catagaagat gtggatttcc cagaggagag
     7501 ctttgatgta atactaagtt cgcttgcgtt tcattatgta gcagactatg agaatttaat
     7561 aaaaaagata tataggatgc tgaaggctgg tggcaattta gtttttacag ttgaacatcc
     7621 tgtttttact gctcatggaa cacaagactg gtattataac gaaaaaggag aaatactgca
     7681 tttcccggtg gacaattatt attatgaggg caaacggaca gctatgtttt tggaagaaaa
     7741 ggttacaaaa tatcatagaa cactgaccac atatctaaat acactgcttt caaatagttt
     7801 tataataaat cagattgtgg agccacagcc gccagagaac atgatggata ttccggggat
     7861 ggcggatgaa atgcgacgcc caatgatgct gattgtatcg gcaaaaaaga agatgtaata
     7921 atatagaaaa aataaacgag gagtatgtaa atgagatcag aaaaagaaat gatggattta
     7981 gtactttctt tagcagaaca ggatgaacgt attcgaattg tgacccttga ggggtcacgc
     8041 gcaaatatta atatacctaa agatgaattt caggattatg atattacata ttttgtaagt
     8101 gatatagaac cgtttatatc taatgatgac tggcttaatc aatttgggaa tataataatg
     8161 atgcaaaagc cggaggatat ggaattattc ccacctgaag aaaagggatt ttcctatctt
     8221 atgctatttg atgattacaa taaaattgat cttaccttat tgcccttgga agagttagat
     8281 aattacctaa agggcgataa attaataaag gttctaattg ataaagattg tagaattaaa
     8341 agggacatag ttccgactga tatagattat catgtaagaa agccaagcgc aagggagtat
     8401 gatgattgct gcaatgaatt ttggaatgta acaccttatg ttattaaagg attgtgccgt
     8461 aaggaaattt tatttgctat tgatcatttt aatcagattg ttcgccatga gctgctgaga
     8521 atgatatcat ggaaggtcgg catcgaaaca ggctttaaat taagtgtagg caagaactat
     8581 aagtttattg aaaggtatat atccgaggat ttgtgggaga aacttttgtc cacctaccgg
     8641 atggattcct atgaaaacat atgggaagca ttatttctat gccatcaatt gttcagggcg
     8701 gtatccggtg aggtggcgga aaggcttcat tatgcctatc cggagtatga taggaatata
     8761 acaaaatata ccagggacat gtataaaaaa tacactggta aaaccggctg cctggatagc
     8821 acatatgccg ctgatataga agagaggcgg gaacagtgat tacagaaatg aaagcagggc
     8881 acctgaaaga tatcgataaa cccagcgaac catttgaggt gataggtaag attataccga
     8941 ggtatgaaaa cgagaattgg acctttacag aattactcta tgaagcgcca tatttaaaaa
     9001 gctaccaaga cgaagaggat gaagaggatg aggaggcaga ttgccttgaa tatattgaca
     9061 atactgataa gataatatat ctttactacc aagacgataa atgcgtcgga aaagttaaac
     9121 tgcgaaaaaa ttggaaccgg tacgcttata tagaagatat cgccgtatgt aaggatttca
     9181 gggggcaagg cataggcagc gcgcttatca atatatctat agaatgggca aagcataaaa
     9241 acttgcatgg actaatgctt gaaacccagg acaataacct tatagcttgt aaattctatc
     9301 ataattgtgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca
     9361 actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg
     9421 agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag
     9481 gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata
     9541 ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga
     9601 aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt
     9661 ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct
     9721 gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct
     9781 ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga
     9841 gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga
     9901 cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga
     9961 aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac
    10021 ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt
    10081 tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa
    10141 gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta
    10201 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta
    10261 tattttactg gatgaattgt tttagtacct agatttagat gtctaaaaag ctttaactac
    10321 aagcttttta gacatctaat cttttctgaa gtacatccgc aactgtccat actctgatgt
    10381 tttatatctt ttctaaaagt tcgctagata gagttctata ttagagatgt aaagagtttt
    10441 ggtgaagagg tttgtgagta tggcttttat aaccagggtg caatgagaag cactaacagc
    10501 agctagctta agaacgcata ggatatactt tttatggaag tcacagagga ggaataaaat
    10561 ttgaacagga ttaacagagt aacctctatt cttattcagc tgcaatcaaa aaaaataatt
    10621 cctgccaaag aaattgcaca gcgatttaat ataagcttaa ggacagttta cagagatatc
    10681 cggacacttg aagaagccgg aataccaatt ggatctgagg ccggaaaagg atattttctt
    10741 gtagagggct tcctacttcc gccggtaatg tttacagcgg cggaagtggg tgcattgatt
    10801 acagcaggga aatttttaaa ttgccatgga gatgaatcat ttataaagga ttttgattca
    10861 gctatgtata aaatcaaatc tattttgaag catggtgaaa aaaactatgc acaggagttg
    10921 gagaacagta ttaacgtgta cagcacatct ggacagaaaa atacattggc ggataacgtt
    10981 attgcagcaa ttcagactgc aatctgcaat aaaagggtaa tatcaattca atatcctgca
    11041 tcaggagggc aagaaccgga aagcagaatg atagagccta tatcactggg gttttatgaa
    11101 cagaactggt acttaatcgg ttttgcaggc taagaaatga atataggaat tttcgtgtgg
    11161 atcggattaa aagcatttgc atagaagaag agctctgtca aagccatgat ggctctttgg
    11221 aacaaatatt aaaacaaatg ctttcatata aaaaactata tgccctacaa tgccgttgta
    11281 aaaattgggg aaaagggata tattgatttt atatctttct caaaataaat ataaaagccc
    11341 tttaaagagg gcttttatat attaatcaca aatcacttat cacaaatcac aagtgattaa
    11401 tcacaaatca cttgtgattt gtgattctta atgatacaat attactatac aaaaaaagaa
    11461 tggggcgtag ttatggagaa ggaaaaacta aaaatacttg aagaattaag acgtatttta
    11521 aacaataaaa atgaagcaat tattatcttg aataattact ttaaaggtgg tgttggaaag
    11581 tccaaattat cgactatgtt tgcttacttg acagacaaat tgaatttaaa agttttaatg
    11641 atcgataagg acttacaggc aacattgaca aaagacttag caaaaacttt tgaggtagaa
    11701 ttgccacgtg tcaattttta tgaaggcttg aaaaatggaa acttggcttc ttctattatt
    11761 catttgactg ataatttaga cttgatccct ggcacgtttg atttgatgtt actgccaaaa
    11821 ttaactcgct catggacgtt tgaaaatgaa agtagattgc ttgctactct tttagcacct
    11881 ttaaaaagtg actatgatct tattattatt gatactgtac caacgccaag cgtttataca
    11941 aataatgcaa tcgtggcgag tgattacgtt atgatccctt tacaagcaga agaagaaagt
    12001 acaaacaaca ttcaaaacta tatttcctat ttgattgatt tgcaagaaca gtttaaccct
    12061 ggactagata tgattggttt tgttccttat ttagttgata cggacagcgc aacgataaaa
    12121 tcaaacctgg aagaactgta caaagaacat aaagaggata atttggtttt ccgaaatatt
    12181 atcaagcgaa gtaataaagt aagtacttgg tctaaaaatg gcattacaga acacaaaggc
    12241 tatgacaaaa aagttttgtc tatgtatgag aacgtatttt ttgaaatgat tgagcgaatc
    12301 attcaattag aaaatgaaaa agaatagaat cacaaatcac aagtgattaa tcacaaatca
    12361 cttgtgattt gtgataggtg atgataaaat aaatagtaag aagaaataga aagaagtgag
    12421 cgatcgtggg aaatttaggc gcacagaaag caaaacgaaa tgatacgcca atcagcgcaa
    12481 aaaaagatat aatgggggat aagacggttc gtgttcgtgc cgacttgcac catatcataa
    12541 aaatcgaaac agcaaagaat ggcggaaacg taaaagaagt tatggatcaa gccttagaag
    12601 aatatatacg gaaatattta cctgacaaac tttaaaagga gtgaaaatga aatggcagtt
    12661 acgtatgaaa aaacatttga aattgaaatc attaatgagt tatcggcaag tgtttataat
    12721 cgggtattaa attatgtttt gaatcatgaa ctagatacta aaaatactcg tttactagaa
    12781 gtgaatcttt taaatcaatt agaagtggca caagaagttg atttatttca acaaccattt
    12841 gaagaattac aagctattca tgagtattgg cggtcaatga atcaatattc aaaacaaatt
    12901 ttgaataaag agaaagtggc ttaacatggc gaatattact gacttcaccg aaaagcaatt
    12961 tgaagatcgt ttagaaaaga atgttgaacg actaactaaa aatagactag cggttgaatc
    13021 gccaaccgct tttttacttg gtgggcaacc agggtcaggg aaaaccagtt tgcgatcagc
    13081 aatttttgaa gaaacacaag ggaatgttat tgtcattgat aatgatacct ttaaacaaca
    13141 gcacccaaat tttgacgaat tagtgaaact ctatgaaaaa gacgtagtaa aacacgttac
    13201 cccttattcg aatcgcatga cagaagcact tattagccgt ttaagcgatc aaggctataa
    13261 tttagtgatt gaaggaacag gacgaacaac agacgttccc attcaaacag ccacaatgct
    13321 tcaatctaaa ggttatgaaa caaaaatata cgtcatggca gtaccgaaaa ttgaatcata
    13381 cttaggaacg attgaacgat atgaaaccat gtatgcagat gatccaatga cagctagggc
    13441 aacaccaaaa caagcgcatg atattgttgt caaaaactta ccgaccaatt tagaaaccct
    13501 tcataaaacg ggcttattta gcgatataag gctttacaat agagaaggag tgaaactcta
    13561 ttcaagctta gaaacgcctt ccattagtcc gaaagagacc ttagaaagag aattaaatcg
    13621 taaagtatca gggaaagaaa ttcaaccgac tttggagaga atagagcaaa aaatggttca
    13681 aaatcaacac caagaaaccc ctgaatttaa agcaattcaa caaaaaatgg aaagcttgca
    13741 gctacctaca ccaccaatac ccaaaacacc taaacttcca ggtatttaaa ccttaaaaaa
    13801 ggaaaagagt agttaccaaa aaacggtaac tactcttttt ttataaaaac atttcctcat
    13861 gttggggtcc cgagcgccta cgaggaattt gtatcgataa gaaatagatt taaaaatttc
    13921 gctgtttttt tctaatttca ttttgaggtg aattaattgg tagttattag aggtgcccta
    13981 tacctaataa tttatctaca ttccctttag taacgtgtaa ctttccaaat ttacaaaagc
    14041 gactcataga attatttcct cccgttaaat aatagataac tattaaaaat agacaatact
    14101 tgctcataag taacggtact taaattgttt actttggcgt gtttcattgc ttgatgaaac
    14161 tgatttttag taaacagttg acgatattct cgattgaccc attttgaaac aaagtacgta
    14221 tatagcttcc aatatttatc tggaacatct gtggtatggc gggtaagttt tattaagaca
    14281 ctgtttactt ttggtttagg atgaaagcat tccgctggca gcttaagcaa ttgctgaatc
    14341 gagacttgag tgtgcaagag caaccctagt gttcggtgaa tatccaaggt acgcttgtag
    14401 aatccttctt caacaatcag atagatgtca gacgcatggc tttcaaaaac cactttttta
    14461 ataatttgtg tgcttaaatg gtaaggaata ttcccaacaa ttttatacct ctgtttgtta
    14521 gggaattgaa actgtagaat atcttggtga attaaagtga cacgagtgtt cagttttaat
    14581 ttttctgacg ataagttgaa tagatgactg tctaattcaa tagacgttac ctgtttactt
    14641 attttagcca gtttcgtcgt taaatgccct ttacctgttc caatttcgta aacggtatcg
    14701 gtttctttta aattcaattg ttttattatt tggttgagta ctttttcact cgttaaaaag
    14761 ttttgagaat attttatatt tttgttcatg taatcactcc tgaagtgatt acatctataa
    14821 ataaatacag aagttaaacg atttgtttgt aattttagtt atctgtttaa aaagtcataa
    14881 gattagtcac tggtaggaat taatctaacg tatttattta tctgcgtaat cactgttttt
    14941 agtctgtttc aaaacggtag atgttttatc tacattacgc atttggaata ccaacatgac
    15001 gaatccctcc ttcttaatta caaattttta gcatctaatt taacttcaat tcctattata
    15061 caaaattt
//
LOCUS       DAZULG010000065        15066 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000065 DAZULG010000000
VERSION     DAZULG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.067"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(464..1030)
                     /locus_tag="ACG9CY_001838"
     CDS             complement(464..1030)
                     /locus_tag="ACG9CY_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4938340.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1030..1986)
                     /locus_tag="ACG9CY_001839"
     CDS             complement(1030..1986)
                     /locus_tag="ACG9CY_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS4938341.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1986..2642)
                     /locus_tag="ACG9CY_001840"
     CDS             complement(1986..2642)
                     /locus_tag="ACG9CY_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4938342.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDHWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3012..5030
                     /locus_tag="ACG9CY_001841"
     CDS             3012..5030
                     /locus_tag="ACG9CY_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4938343.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5100..5306
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5457..7871
                     /gene="leuS"
                     /locus_tag="ACG9CY_001842"
     CDS             5457..7871
                     /gene="leuS"
                     /locus_tag="ACG9CY_001842"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS4938344.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8219..9772
                     /locus_tag="ACG9CY_001843"
     CDS             8219..9772
                     /locus_tag="ACG9CY_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS4938345.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10153..11937)
                     /locus_tag="ACG9CY_001844"
     CDS             complement(10153..11937)
                     /locus_tag="ACG9CY_001844"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938346.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11952..13673)
                     /locus_tag="ACG9CY_001845"
     CDS             complement(11952..13673)
                     /locus_tag="ACG9CY_001845"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938347.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13773..14534
                     /locus_tag="ACG9CY_001846"
     CDS             13773..14534
                     /locus_tag="ACG9CY_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4938348.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            complement(14876..>15066)
                     /locus_tag="ACG9CY_001847"
     CDS             complement(14876..>15066)
                     /locus_tag="ACG9CY_001847"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938349.1"
                     /translation="MLEEKSLNTYNYFISKKMLSVVSPIAEESHSRDYLIKKYVYSIH
                     LFIFALLTYCNNVSNNVF"
BASE COUNT         4908 a         2759 c         2973 g         4426 t
ORIGIN      
        1 tattttttct aataaattct tccatcataa aaatcacctc aaaacgatta tacgaactta
       61 cgttcttgtt gtaaagcgat atttgagggg caaaaaaggg gcataatttg actgatatta
      121 agttagctat tgatatatga atgacataca aaaatataaa atcttttaaa aatgcttata
      181 tatcaatgtt tttgccattc ttttatatat ctgttatatg tcaaaaaaat gtgttcaatt
      241 tcaatttgta tttgaataaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
      301 agccaatcaa aaacagcttc tcatgttttt gatttggttg gacttattac cgcagatata
      361 agcttttgag caccgtgaat aaagaggtta tgaaatcaac gctctaacct gagtattaga
      421 gaaaaaaacg caaaacatat catttatgtt ttgcgttttt tatttatctt tttttctcaa
      481 tacaatataa aataggcggc tggttctttt ggttgataaa ccgataattc agcacattat
      541 atgtttcctg tggcagtttg gaacaaaaag aatcaaccat atccagttct ttttctccac
      601 cttcatgacc atagtagacg acaaggatca atcgtccgcg aggcacaagt cgtttcaata
      661 tttcttccat agctgtgcgt gtagtctctg gcatagtgat gattgcttta tcactttttg
      721 ggagataccc tagattgaaa atccctgctt tgagattttg ttgttcagct atcacttctc
      781 ctaacgtttc gtggccttgt agaaacaatg agacacgttc ttctaactga cgctgttcta
      841 gcctttcacg agtagcatcg attgcttgtt gctggatatc aaaactatag acatgaccag
      901 tttttccgat gtgttctgct agaaaaaccg tatcgtggcc attgcccatc gttgcatcta
      961 cgacgtgatc ccctggttgc aagatttctt gcagtaatgt atggctgaaa tgcaaagcag
     1021 tttgcaacat cagacgctca cctttttccg tacatcatat tttccttgga agctatctct
     1081 gcgaagaagt tcttcatcaa ttgcattcaa gacttcccat tttttcaagc tccacattgg
     1141 tccaatcaag gaatcccacg gcgcatcccc tgtcaaacga tggatgatga tctcttttgg
     1201 tatcatttcc agctggtcgc aaatgacatt gacatagtca ggacggctca ttaattgaag
     1261 gcgaccttca tgatagtccc gcaacattct cgttttgcgc atcagatgca tcaaatggag
     1321 cttgattcct tggatatctg aatctaaaat ggtgcgtctg acattttctt tcatcatttc
     1381 taaactttcg cctggtaatc cgttgatcaa atgcgtacaa acacggatat tatgtttgcg
     1441 taatttagcc acgccatcta aataagtttg ataatcatgt gctcggttga tcagttttga
     1501 agtttcttca aaagttgttt gcaatccaag ttctacccat aaatacatcc gctcgttcag
     1561 ttcagcaagg taatcaacta cttcatctgg cagacaatcc ggtcgtgtac cgactgataa
     1621 tcctacgaca cctggtaaat tgactacttg ctcaaatcgt tctttgatta tttcaactgg
     1681 cgcatgggtg tttgtaaaat tttggaaata aacgatgtat tgttggacat gaggccattt
     1741 ttgatgcatc atgtcgattt ctttctggaa ttggattgga agagggtctt caggtgcaac
     1801 gatcatgtct cccgaaccag atacactgca aaaagtacaa ccgccatggg caacagtccc
     1861 atctcgattc ggacagtcaa atcctccatc gatcggcacc ttgaaaattt tcccgccaaa
     1921 ctcatttcgt aaagcgtaat tccaagaatg atatctttta tttccttctg aataaggaaa
     1981 agtcattaaa agtccttctt tctgtaagag ttatctattt gttttctttt ttctctatac
     2041 actggataag tcaggcacag ccaagaaaga ctgagtaagt agccgccgat gatatcagta
     2101 ggaaaatgaa cgcccagata aatgcggctt atcccgacac ttactataat aaacatcatc
     2161 agcaattgaa gcaatgtgcg taaggttctt ttttgaacaa aagctggaag cagaaatagt
     2221 aaagaaccgt aaagaatcat actgcctgct gcatgtccac tagggaaact gtaacttgtt
     2281 tcaactacta agtgcgccaa tgaaggacgc tctcgcatga aaaatagttt gatcaccggg
     2341 ttgatgattc cagcaattac aataaatcca gaaacgaacc aaaatgtttc tgcccttttt
     2401 ttcttatacc acatccataa agccgcacaa gcaaataaaa tgatgacggt aagaggatta
     2461 ccgaatttag tcataaagag ttggtagctg ttcagatcag gataagaagc tctgacaatg
     2521 cctgtaaacc agtgatcgaa accagaaagc cacgacagat ggaactttac gatccagccc
     2581 aaaatgaaaa acaggacaag aaagaagcta ccagccacct gaaatgatgt tttatatttc
     2641 atacaattcc tcctttgaag caatgactta ttatagcatt tttggtccag cgaaaaaaac
     2701 ggtaaagaga taaaaccaat gtatgattcc tctttgaata accaatattc ttataaaaaa
     2761 aataactgta atcggaaaga cattttttgg taatgcaaat gaaatgttag ggaaataaaa
     2821 tcagtgtttt tcgaaatcct tttaaacaag aacgtagacg taggaagaac ttgaaaatga
     2881 acagaagttg ttcattacgg taaattacaa tatgattaca aaaaacaaca aagctcttaa
     2941 ataactttta ataaatatta agagaagtta taattattaa taatagataa attgaagttg
     3001 gaggatagaa gatggaaaac attgcaagaa aagaacggag acgtttagaa cagaccaaac
     3061 gttttcgaaa agcaaaacga ggggcagcaa tcgtgggaac tgcgatggta ggctgttctg
     3121 tcgctgcccc attagtacag ccggttcctg ttcaagcgga tgaaacacct gttcagttcg
     3181 gcgcaagaat caatacagcg gcatttatca cagaaattgc tacttatgct caaccaatcg
     3241 ctcaagccaa cgatctatat gcatcagtga tgatcgcaca agcagttgta gaaagcggtt
     3301 ggggcagtag tgcgttatct caagcacctt attataatct ttttgggata aaaggcagct
     3361 atcaaggaca aaccgtctac atggatacgc ttgaattttt aaataatcaa tgggtaaata
     3421 aaaaggagcc tttccgacaa tatccttctt ttgctgaatc tttcaatgac aatgcctatg
     3481 tacttagaaa tacttcattt ggcaacggtt attactatgc cggtacttgg aagagcaaca
     3541 cgaaatccta tacagatgcg acagcttgtt tgacagggcg ttatgcgact gatcctggtt
     3601 acgcaggcaa attaaataat atcatcgcaa cttacggatt gacccaatat gatacaccag
     3661 ctagcggaaa cgcaggcggt ggagcgacta caggtggcaa taatactgga agtgcaggta
     3721 gtactggcgg ttcaagcaat tctggtaata caaactcagg ttcagcaact tcgaccactt
     3781 atactgtcca atctggtgat tctgtttggg gaatctcaaa taaattcggt atcacaatgg
     3841 atcaattgat ccaatggaac aacatccaaa acaatttcat ttatccagga caaaaactga
     3901 caatcaaagg tagccaggaa aatggctctt cgacgaataa ttccggtaac aataccaact
     3961 cttcaggaaa tgctggtaca tcaaacggag gacaaacaac tggtgcgaaa tacactgttc
     4021 aatctggtga ttctgtttgg aagatctcaa atgatcacgg aattacgatg gctcaattga
     4081 ttgaatggaa caatatcaag aacaatttcg tttatccagg acaacagcta atcgtatcaa
     4141 atggcggttc ttctaatgct ggagcaaata ctggctcaaa tactgggaat acaagtggct
     4201 ctagttcgtc cagctcaagt tcttctaata cagcttcagg aacgaaatat acagttaaag
     4261 ccggagactc tgtttggagc gtctcaaata aatacggaat tacgatggct cagctaatcc
     4321 aatggaacaa tatccaaaat aactttatct acccaggaca acaactgact gtttcaaaca
     4381 gtggatcaac aaacagcacg tcaggtaata caggttcgtc aaccaataca aataacagca
     4441 gttcagttaa tactggcagc agttcgtctc aaggcactta taccgtgaaa gcaggtgaat
     4501 ctgtttggag cgtggcaaat aaaaacggca tcacgatgga tcagttaatc gaatggaata
     4561 atatcaaaaa caacttcatt tatccaggac agcaattgat cgttaaaggt ggaacttcgg
     4621 caaatacaaa tacaggttca actacttctg cagcgaaacc aaatacaccg aatacgtctg
     4681 ctacgacaag tacatcatcc actggtaata cgatgtatac cgtgaaagcg ggcgagtcag
     4741 tttggagcgt agcgaacaaa catcatatca cgatggatca gttaatcgaa tggaataata
     4801 tcaaaaataa tttcatctat ccaggacagc aagtgattgt gaagaaaggt tcatctcaat
     4861 caacaaacca acaatcagtt actggttcga aaacctatac tgtgaaatct ggagaatctg
     4921 tttggagtgt tgctgacagt catggaatta caatggctca actaatcgaa tggaacaata
     4981 ttaagaacaa ctttatttat ccaggtcaaa cgttgattgt agctaaataa aatagctgtt
     5041 cgaagaaata acgattgaat tccacaaaaa tatttgttac tataaggcta cttaaatatg
     5101 acaacacttg aaaaggaagt agtaaaaaga ctgaagtttc agagagcgca tggctggtga
     5161 gaatgtgcac ggatgctttt gaactcgcct ttgagttgcc cttgctgaat acccgagtaa
     5221 gcaagcggcg gtgacggccg ttacaacgtt cgaggccttt tcactaaggt aaaattaggt
     5281 ggtaccgcgt agattacgtc ctataaaaag agcaatcttt ttataggatt tttttatttc
     5341 ttttgtgaag tttgagaaaa caaagcaaaa ggaaataaaa ataaacagat tctgccggtt
     5401 ttttcgttat atttataata gaaacaagtg ttttcaatag aacaggagga cagcaaatga
     5461 gttacaatca taaagaaatt gagaaaaaat ggcaaaaata ctgggcaaaa acgaatactt
     5521 tcaacaccca tgatgatcct gaaaagccaa aattttacgc actggatatg tttccctatc
     5581 catccggtca agggctacat gtagggcatc ctgaaggtta tacggcaacg gatatccttt
     5641 cacgtgtgaa aagaagtcaa ggatacaacg tgctgcatcc aatggggtgg gatgcattcg
     5701 gtctgcctgc tgaacaatat gcgctagata caggaaacga tccagctgag tttacccaaa
     5761 aaaatatcga aacattccga cgccaaatca attctttagg atttagttat gactggaatc
     5821 gcgaagtgaa tacaactgat ccggagtatt ataaatggac acaatggatc tttacgaagc
     5881 tttatgaaaa aggtctagct tatgaagcag aagtagcggt aaactgggta cctgaattag
     5941 gaacagttat ttcaaatgaa gaagtgatcg atgggaaaag tgaacgtggt ggctatgatg
     6001 tagtcagaaa accaatgcgc caatggatgc tgaaaatcac tgcttatgct gatcgcttat
     6061 tggatgatct agaattagtt gattggccag aaagcattaa agaaatgcaa agaaattgga
     6121 tcggtcgttc agtaggagca aatattgaat ttaaagtggc tggtacggat aaatcctata
     6181 ccgtatttac gacacgacca gatactctat tcggtgcaac ttactcggtt cttgcacctg
     6241 aactagatct tgttagagaa attacgaccc ctgaacaaaa agctgctgta gaagcttata
     6301 tcgaagagac agctaaaaaa tcagatctaa agagaacgga tcttgcaaaa gaaaaaacag
     6361 gagtatttac tggtgcttat gcaatcaatc cagttaatgg caaggaaatc ccaatctgga
     6421 tcgccgatta tgtattatct tcatacggca caggagccat catggctgtt ccagcacatg
     6481 atgaacgtga ctttgaattc gctcaaactt tcggactaga aattcttcca gttatcgaag
     6541 gtggagatgt tgaaaaagct gcttacacag aagatggtac gcacatcaac tctgaatttc
     6601 tagacggcat gaacaaacaa gaagcaatcg ataaaatgaa tgaatggcta gaagaaaatg
     6661 gcgtagggaa aaaagaagtc agctatcgct tacgtgactg gctattctcc cgtcaacgtt
     6721 actggggtga accaatccca atcatccatt gggaggacgg aacagtcaca gctgtcccag
     6781 aagaagagct accattacgg ttgcctaaga caaaaaacat caagccaagc ggtactggag
     6841 aatctccatt agcaaatatc gaggagtggg tcaatgtcgt tgatcctgta acaggtaaaa
     6901 aaggccgtcg cgaaacgaat acaatgccac aatgggcagg aagctcatgg tattacctgc
     6961 gttatatcga ccctcataac aaaaaagaac tagcagatta tgaaaagttg aagcgttggc
     7021 taccagttga catctacatt ggtggagcag aacatgcggt acttcattta ctatatgcac
     7081 gattctggca taaattctta tacgatatcg gtgttgttcc tacaaaagaa ccattccaaa
     7141 aactatacaa tcaaggaatg atcttaggcg aaaacaacga aaaaatgtca aaatctcgtg
     7201 gtaatgtagt caatcctgat gatgtggtcg aaacttacgg tgcagataca ctgcgcatgt
     7261 acgaaatgtt catggggcca ttagatgctt cgattgcttg gagcgaaaat ggcttagaag
     7321 gaagccgtaa attcttggat cgtgtatggc gtttgatcgt tgatgagaac aataaaatgc
     7381 gtgaccgtat cactacattg aatgatggca agcttgataa agtgtatcat caaacagtga
     7441 aaaaagtgac agaagactat gaaaatcttc atttcaatac agcaatctcc caattgatgg
     7501 tctttatcaa tgaagcctat aaagtggacg ctctgcctta tgaatatatc gaaggatttg
     7561 ttcaactact cgcaccgatt gctcctcaca tgggtgaaga actatgggca atccttggaa
     7621 atgatggtgg tatttcttac gcaccatggc caacatacga tgaagcagca cttgttgaag
     7681 acgaagtaga agttgttttc caagtaaacg gtaaagttcg tgcgaaaagc aatgttcccc
     7741 gagacttagg aaaagatgag ctagaaaaag cagcattagc caatgaaata gtgcaagaat
     7801 atattgaagg caaaacagtc agaaaagtta ttgcggtacc aaacaagtta gtcaatatcg
     7861 ttgctaatta attggctctt tgtcaataag gactgatgag ttgtgcaaaa tcaaatctgg
     7921 gccagaatga acttcattct gtctcagatt ttttgttatt tcaaattgat taattgattg
     7981 atcaaaaagg ttttgatttt catgttctca aacacgttca atgtcttttt cttctgaatg
     8041 gtcttgtaag agatctctgt aatcatcaaa tttttgtcta ttaatcttag tattttttga
     8101 tgtataaact acctgaaatt gagagtacat accaaattca taaatttttt attagtggga
     8161 ctagtgattg tatggatttt gacgcataat gaatgaagta tgagaaaagg agagacaaat
     8221 ggatcaagaa ctatttatta tcgaagtatt atcagctaaa caaaaaatta tcgagaaagc
     8281 gaaaaaaaga gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt tctctggttt
     8341 gctttttttc ggagctgtat cgttcctata tcgccatatg agtgcagtag tccgttcagc
     8401 tgaagggatg caaaaattaa ataatttgta tcacttttcg tggggagttg gcggaataac
     8461 aattgttgtg cttattgttt ttcttctact tatttggctc cgttaccatc aattgtcgcg
     8521 tcatttcgat caatggctta gtcaaagagc acaacagttt ttacataata aaccaacttc
     8581 tcaagatgat gactatcttt attttgagaa gacttcgacg agtcaagctt ttcggataca
     8641 aaaaagtgat tgccagttat tgctgagatc acttgatgat ttacctatct acttaggcga
     8701 aagcaaatca aaattcggaa tgaacacatt ccatattttt ttactagaaa acgaacccag
     8761 atgggaaaaa agccagtctt cgcctggtgg atacctgaca actaagatga gtacattagt
     8821 cactggtttt ttcctcattc ttttagctgg aacaatctat tatgggagat acactcagcc
     8881 aaaaatgtct tctcatataa atgctgcttc tcacatgaca atgaactcaa ctaacgatac
     8941 gcatatacaa aagacaaatg aagggttgtt agctgatcct aagcaaaagg taaatttgac
     9001 aacacaaaag ataaatgatc tcaaacttga tacagagacg cactctctct atatgacaac
     9061 agatagtggt gctaattggg tatttgtgcc gattgattcg caatggttgc gttttggcga
     9121 atacaccctg acaacggggc tgattcctga aggttattgg atggacaaca catatgatat
     9181 ttccgccgat ttctcatggt ttatctattc ttctggggat gatgtacgtc tgctgacttc
     9241 aagagataat ggtaaaacat ggcaaacgtc tattatcacc caatcagttc agccgatacg
     9301 ttatcgaaaa gcggtatttt atggcaacga tggtctattt atctgtacat cagccagtgg
     9361 gatgtctgca gaaactttgt acatttacac tactatggat ggcggaatca cttggataaa
     9421 aagtggaagt acaatgatca atcagccagt acaaaatgtc agcttcgtta caacttcttt
     9481 aggttttgtt tcgacacgtg aaagtttgtt ttatacaaac aacggaggac gctcattcaa
     9541 agaatcagtt ctgtcgattc cggaaaacta tcagacaggg gggatagatc tttttcaatc
     9601 accaaatgaa gttactcaaa taagcgcgaa tacgttagag acaaaatttt atttgttgaa
     9661 aaaaggacca atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa ctgataacgg
     9721 ggaaacatgg caattcgttc agcaactatc gcaagtagaa caaggagatt aaagaagaga
     9781 gattgtgagc ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa acagcttctc
     9841 atatttgttg aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa gaagcgattt
     9901 tgaagtatag cttctcgtat tttacaagat cgtgtcttat tgccgatagg ttgtctcttg
     9961 aacaccgttt attcgtaaaa agggattgtg acaagtcttt tgtcactact taccgaacga
    10021 aactgaagga gctatccaaa aagtatcatt agtattattc tttgtgtttc tagtggcacc
    10081 actagtacat ttaaaaaagc tatcgatcat ggactaatca tacatgatcg atagcttttt
    10141 agataatgtt tgttacgcta tctgaaattg actattataa agactggcat aaagcgttgg
    10201 tgattggagt agttcagaat gtgttccttt ttcgacgata tcgccattct tcattacaag
    10261 gatcatgtct gcattttcaa tcgttgacaa acggtgtgca atcacaaaac ttgtcctatc
    10321 ttttgtcaca gcttccattg cttgctgaat caatgcttct gttcgtgtat ctacgctaga
    10381 agttgcttca tctaaaataa cgacaggagg attcgctaga atgactcgag caattgtcaa
    10441 taattgttgc tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg tatcatatcc
    10501 gtctggcagt gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt cgataatttg
    10561 ttctcttgtg gcatccattt tcccgtaggc gatattatca gctaccgttc cttcaaacag
    10621 ccaggtattt tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg aaagcttggt
    10681 aatatcaaga ccgtcgaatt cgatctttcc tttattcagt tcataaaaac gcatcagcag
    10741 attgactaaa gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg tttgttttgg
    10801 ctttactgaa aaatcgactt gattcatcaa tattttatct gatgtgtaac caaactgtac
    10861 atttttgaaa gctatttttc ctttcggttg ttctaaaagt cttgcatctg gaaggtctgg
    10921 aatttcatct gtttcatcta atatctcaaa tatcctatcg attgctgcca ttgcggattg
    10981 gatcgagttg atcacataag aagcggtaga aattggttca gaaatctgat tgatgtactg
    11041 aagataagct tgtaagaagc cgatcgtcac accacctgat agaacaagca tagcaccgaa
    11101 taccgcactt atgatgaagg cgagttgatt aatgaaacga atggccggat agatagcaca
    11161 gttcataaat tgagcacgtt taaaagcgtt gtattgcttt ttatttactt cgtgtatagc
    11221 agcttctgca tgagcttgct gattgaatgt tttgaccact agattaccag ctaagatttc
    11281 ttccattcga ttatttaaaa cacccagttc atactgattc tgttcagaaa tctgtttgtt
    11341 tttgttcgca atcttagttg taacaattga actgccaacg atcagcaata agacgagcaa
    11401 agtaagcttg ctttcgatat aaaacagcat cagaccagca gcagctattg ttacgacaga
    11461 agtaaaaaat tgattgatac ctgtcaataa cacttgagaa agttgattga gaccggtagt
    11521 tgtccgacta atgatgtcac ctacttgatg attgtcaaaa aacgacatcg gtaatgtttt
    11581 gaattttttt gtgatttctt ttcgcaccct caatgtgatc ttttcgctta acgctgccat
    11641 tgtccgttcc tgaataaatg aagtgatgct gcttaagact gaacagccga tcagcaataa
    11701 gacagggaac aacaatgctt gtttgatatc actgaaagaa agctgtccaa tacctgtttt
    11761 tcttatttct gataagagat catcgatccc gattcccatg atcaagggca tggctacgac
    11821 taataggttc ccgaataaac tgcatagaaa caatccgtaa aaatatttat gttcaggttt
    11881 gatcattttc caaaaacgta tcaaagcacg cgtgttcaac gtttttttct tttccatcgt
    11941 atcccttctt tctatattcc ttgtgatttt gcaaactcct gataagtttg gcaattagac
    12001 agtagttctt catgagtacc ttgtccaacg atttttcctt catctaaaac aatgatgtta
    12061 tctgcttgtt ggattgtact gaggcgttga gcaacaatca cgagagtttt ctccgacatg
    12121 tgtttatgaa gggctgctct taatgctgca tctgttttat agtctaaagc ggagaaacta
    12181 tcatcgaata catagatctc tgctggtttg atcaacgctc gagcaataca tatccgctgt
    12241 ttctggcccc ctgaataatt agaaccgcct tgagcaacaa agctgtccaa tccgtcagga
    12301 agggtgtcca ctagttcttc taattgagcg atcttgatcg ctttagacaa ctcttctgtt
    12361 gaggcatatt catttcccat ctgcaagttg cttaaaattg tcccgctgaa taagaaagcc
    12421 ttctgaggga cataactgat gtgggagcgg atctgttctt gagtcatttg agtaagagga
    12481 ataccaccaa gggttacttt tcctatcgta gggtcactaa gtctcaagag aagtttcgcc
    12541 aaactacttt ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc tttaggtatt
    12601 gtgaaatcaa tatcttctag aactggatca gagatttcag tatatttaaa tgtgacatgt
    12661 tcaaaagcag ctgcatttgt ctgatcaaag gttggttcag ctactgtttt gatcgtctct
    12721 tcctcaattt cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga ggcaaacgat
    12781 ttagggataa tgaccaatac catagctgcg atcatcaagt aagcaagtgt cagcatagag
    12841 tattcgacta cagcagtcac gacaccgatt tgtaatgctc cttctgctac aagatttccg
    12901 ccaaaccaca aaatagaagc ataaacgatt cccatgagca aaaaagcaat tggagtgacc
    12961 caagcaaata ttcggttcac tttgatcatt tgattagcat agtcagcaaa gctcttattc
    13021 gttcgctttt cttcatatga ttgattatca aaggcacgga tcatattaat cccagtaaag
    13081 aattctctca atgtgatggt gatccgatcc atttttggct ggatactcaa agataaaggt
    13141 gcccctcgtt tcatcagtcg ataaacaatc catacataaa aaacaacaga gatcaatgta
    13201 acccatgtta gtaatagaga ataacgaaac atcatgagaa tggtgaaaac gcaaataacc
    13261 ggtgctggta agatcatttg gcaaaataaa acaatcattt gctggacatt atcgacatca
    13321 ttcgtcattc ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt cattgacagc
    13381 tgttggaatt tacggaaata tttctcccgc gtttggaatc caaacctagc ggaagccaat
    13441 gctgaaaagt aactagaggc aactgcagcg attgttccta gaaacgctac aagcagcatt
    13501 tcaactccaa ttttttttat aactgaggct tcgccacttg ctatacctgt gtcgatcagc
    13561 tttgctacta attttggtac acctaatgta ccggcgattt ctaaacagat catcacaaag
    13621 gtaaggagag cgagtccttt gtttttctgt aagaatgttt ttaagataga catattattc
    13681 ctcctttttt cctgatactt ttcgattcta taccttcaag taacttgaag gtcaatagaa
    13741 aaaagaataa actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg attggccaag
    13801 tatcggaact atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa
    13861 tccgttcaag tagacaagaa gagaatgatt atcgattgtt tgatatcgat gatattttta
    13921 tgctttatga tatcgttttt tatagaaaat tagatatacc gatcaaacag atgaagaatt
    13981 tatatggaaa aacattgacg gagttatatg agacacttga tgaaacagaa agaagaatcc
    14041 ataaagaact ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa aaaaaacagt
    14101 tgaaattgat gatcgataca aacgaggaag aatttcctgt agaagagatc ccttttgact
    14161 gtatgatctc aacggagttt gaagatattg tggaaatcaa aaaatttcta cctaactatt
    14221 caagctttgg gatgatgagt atgccagctt ccagttcaca gacgatgtat ggatttttta
    14281 tcgaccctag tgaagtacat ttatttactc aagacgtgat ttgggagaaa aaagatacgg
    14341 ctgtttatcg tcgattctta ttgaaatccg agatgaacca tgcggaacgt aataatattt
    14401 tagaaatcag agaaagaatg tatgaaaaag gatggaaaac aggagaagtg atcggccagt
    14461 atctattgac gaatacggat gaaaacaata tcagaactga atattatcat gcatggatag
    14521 aaatgaaaaa ataggaaata tgtgtttaag ataactcgaa aatgatggtt gttttagtta
    14581 taaagaaaaa ttttctggtt cgattacggg tttaatgtgg gggattatta gtcgtggata
    14641 gatagaattt tagtttttta gaggaaatat gtctttaaaa atggaatggt tgcaattctt
    14701 agcgatatat acgatgtaat tgtcctctta aaaaagtatg gaaaaataat tgtattgggt
    14761 gttatgtata attcatggtg aactgagact aatgagaaga aaaattcata ttgttatgag
    14821 tgagattgta tcagatgaga atgataaatt aaataggtgc ataaaagcat tacacttaaa
    14881 atacattgtt gcttacatta ttacaatatg ttaataaggc aaaaataaat agatggattg
    14941 aataaacata ttttttaatc aaatagtccc tactgtgact ttcctcagct atcggtgata
    15001 ctaccgatag catttttttg cttataaaat aattataagt atttagagat ttttcttcta
    15061 acattc
//
LOCUS       DAZULG010000066        15047 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000066 DAZULG010000000
VERSION     DAZULG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.068"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..633)
                     /locus_tag="ACG9CY_001848"
     CDS             complement(52..633)
                     /locus_tag="ACG9CY_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS4938350.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(639..1898)
                     /locus_tag="ACG9CY_001849"
     CDS             complement(639..1898)
                     /locus_tag="ACG9CY_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4938351.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1900..2946)
                     /locus_tag="ACG9CY_001850"
     CDS             complement(1900..2946)
                     /locus_tag="ACG9CY_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938352.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3181..3456)
                     /locus_tag="ACG9CY_001851"
     CDS             complement(3181..3456)
                     /locus_tag="ACG9CY_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS4938353.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3699..5009)
                     /gene="der"
                     /locus_tag="ACG9CY_001852"
     CDS             complement(3699..5009)
                     /gene="der"
                     /locus_tag="ACG9CY_001852"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS4938354.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5191..6420)
                     /gene="rpsA"
                     /locus_tag="ACG9CY_001853"
     CDS             complement(5191..6420)
                     /gene="rpsA"
                     /locus_tag="ACG9CY_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS4938355.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6555..7235)
                     /gene="cmk"
                     /locus_tag="ACG9CY_001854"
     CDS             complement(6555..7235)
                     /gene="cmk"
                     /locus_tag="ACG9CY_001854"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS4938356.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7284..7919)
                     /locus_tag="ACG9CY_001855"
     CDS             complement(7284..7919)
                     /locus_tag="ACG9CY_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS4938357.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7985..9388)
                     /locus_tag="ACG9CY_001856"
     CDS             complement(7985..9388)
                     /locus_tag="ACG9CY_001856"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS4938358.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(9375..10406)
                     /locus_tag="ACG9CY_001857"
     CDS             complement(9375..10406)
                     /locus_tag="ACG9CY_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938359.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10437..10658
                     /locus_tag="ACG9CY_001858"
     CDS             10437..10658
                     /locus_tag="ACG9CY_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS4938360.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10823..11560)
                     /locus_tag="ACG9CY_001859"
     CDS             complement(10823..11560)
                     /locus_tag="ACG9CY_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFS4938361.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(11713..13053)
                     /locus_tag="ACG9CY_001860"
     CDS             complement(11713..13053)
                     /locus_tag="ACG9CY_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4938362.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(13290..14813)
                     /locus_tag="ACG9CY_001861"
     CDS             complement(13290..14813)
                     /locus_tag="ACG9CY_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4938363.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(14863..>15047)
                     /locus_tag="ACG9CY_001862"
     CDS             complement(14863..>15047)
                     /locus_tag="ACG9CY_001862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938364.1"
                     /translation="SSPFKRRKCLFIKKRKRYNEKEESFKNLKKEEGIGKNELFEQKF
                     YKMRVIIFVLILFLIK"
BASE COUNT         4263 a         3226 c         2504 g         5054 t
ORIGIN      
        1 gattccacat ccagctgacc tttgtctttt ttagtactat actattttta actaattgtt
       61 atgttcactt acccggttac ttttcttttg tgtctgcaaa aggatctttc ggttaacttc
      121 atcagataat ttcaaaaaag catccctatc tccatcttct agtgcttgat cgatttgagc
      181 ataaagcatt tttagttttg cctcttcttc tttttcttga aaatatcgtt caattaattc
      241 agtatcttct tcactgactg aagaatccca agaagaaaat ggattgtctt ctaagataga
      301 tagatattct cttgtctgcc aagcattttc gaatatacat tccacatata aaggcttttt
      361 ccaattcaaa cggatttcat gaaaaatctg gtctgtgtcc gtaaattctt ttccctgcaa
      421 aaacaatttc atgggttccc cttcaacaga tagatcactg atctgcagtc ctcgatccgc
      481 aaaattcgcc ccttcaacaa agtggacatt gcttaaaatc gcttcatgat tggcaagata
      541 attcaagatc caaaaaactt ctcttcggct aaaagataca tgattgacca tccacgttaa
      601 aaaattcttc ttttcccgta catcaattag catttttctc acctctctgt taggtcatct
      661 aataatgaca gtgcctccat atctcctggt tcaagtgtta aataataagt caataattct
      721 tgcgctcttt tcagctgccc ttcttctctt aagaaaaagg catactcttt caagaaatct
      781 ggttcatgtg atagttcatg ataagcctgt tcatagtgta cagctgcaac ggtaaagtct
      841 tctaattcat tataagcatg ggctaggttc cattctgcat aaggattagc agtttcttcc
      901 atctgattga tcacttgtat cacatcttca tatctttcat catccaagta aagattactt
      961 aaagtaagta atgtttcatc ttgtttgtct cctacttcta aagctttcag taaaagttct
     1021 tctgctcgct gtctatcatg cagacggaaa gcattttccg aggccaggtg ataaagttcg
     1081 acttggtaag gattttcttt gattccttct tctaacactg tttgtgcttc ttcaatcatt
     1141 tcttcttctt gaagagcttg tcctagatat agatataacg attgataatg cggattaact
     1201 tctcgaagtt cttccagata gttgattgcc tgctgatttt ctttcaattg aagattcgta
     1261 aaagctagct ggaacagtcg atcatctgtc ctttcttctt tcagtgcttc ttctaaaggt
     1321 acgatagctg cttcgaattc tccttgcata cttaaggatt gtcctaaacg ttcctgcatc
     1381 gagattcctg aaatttcatt tatcccgctt gccaacaatg tttcataaac cactgctgcc
     1441 tctttgaaac gatcaactga aaaatacaat tcagctagcg caaactggat caatggttca
     1501 tcaggaagca acccagctgc ttctttcaat tttgcttcac tcacttcagg gattccgata
     1561 acttggtaca aatcagctat cgccaataag ctttgcggat agtaatcact ttgcttatcc
     1621 actttttcca ggtattcaaa agctaagtcg atctcattat cttcgattgc aatttctgct
     1681 aacgggatgt tcagtccgtc attttctggg tattggttga tcagttgttc aaaaatctgt
     1741 tttgcttcga tcaaaaagcc aatagccagc aattcttcgc ctagttcagc taatatatct
     1801 gctgggtctt cttttaatgc tttttctaaa aagatttgtg cttgtgacaa gtcttcatct
     1861 gataaagctt gcaacatttt ttcgctattt gtattcattt caattccctc ttatctcact
     1921 gacgatttgt tgaaataccg tcattgttct caataattct ttttcgtttg gatgttggta
     1981 tccgtctatt atacctattt tttctaaaac aagtattttt ttgctttttt ctgccaaaaa
     2041 aagaactttt tccaaatact ccatctttga aatctgttcc agccagtgga ttggatagcc
     2101 aagcaagtcc agccacacaa aaaagttttt tagttggaaa tcaaaaggat atgcttggag
     2161 attccacacg aaatgaaaca tcattcctaa aaattttttc atactagcag aaagtagatg
     2221 gccattttcg gtcagataaa aagcttgttc aaacagttta ccataatttg acaggttttc
     2281 tgcctctaat tgataaaaat agatcatttc ttcgatcatc ccggcaaacg gccgagtcgt
     2341 taattgcttt ttatttggat agtttttata cagattttgc aaaaaacgat gatcacaaat
     2401 cattccgcaa cagataaaag tcaaaaagtc aatctgcttt ccgtccacct gccgctcgct
     2461 gattgtttga tcataaacga tgcgttctgg cagattgatg gtttgcagca ctacacgatt
     2521 tggtagctgc aaaatcgtcc gctgcggtgt taatgctcgg cttagagaac ggatcgatac
     2581 agggatcatc catagtgatg aatcaagggt tgtatgtttt tgaaaaaaac ctgccagatc
     2641 catgatccct tcatttccaa atccgatgat aaacatggat tgattttcgg gatagcgttt
     2701 gacgaaatca agcaggttct ttaattctgt taaatgattg cattgagtgt tggcacaaat
     2761 ataccagtca ttgtttgctt gatttgcaaa taacgggttg atcttatctg caaacaaatc
     2821 ataatagcgc tgattcgtta aaaataagat tggctgatca atggaaagtt ctcttagctg
     2881 actgactagc gtttctccgt aaaacacttt cgtcaacgca tcttctactt gataatcaag
     2941 ttccatacgc atccctcctc ttcttatttt tattttacca ttagtaagca aattctagaa
     3001 aagataagag caaagctcac ctcataactt gcaaataaat aaaaattaaa taaaatacat
     3061 gtgttttcta tgtatgacat acggattcgg aatctctgtc tcttatttat tccttttctg
     3121 ctaggaaata aaaagaaaag cacttgtagc cctagagcca caagcgcttt ttcaactgtc
     3181 ttatttaaca gcgtctttta atgctttacc tggtttgaat gcaggtactt tgctagctgg
     3241 aatttcgatt tcttcaccag tttgtgggtt acgacctttg cgagctgcgc gttcgcgaac
     3301 ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg ttagctaaag catcttgaat
     3361 agttgaaaat acagcatcta ctgctgcagt tgcatctttt ttagttaaat cagtagctga
     3421 agcaactttt tcgattaatt ctgctttgtt tgccatggat agattcacct cctccacaag
     3481 agatatgata gaaatatcat ttagttaaaa ctttctgagt ggtccagaag tttaaaaaga
     3541 aatattgatt aacttcaata ttctgttatc acgatatcat atgaaccgct taatagcaag
     3601 gtttttacca attttcacat gcttttttta gtcatagaaa cgtaacaaac gaaactgtct
     3661 ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct attttctcct acgagggata
     3721 attcggatgg acgtcccttc aaaagtaaat gcttttctga tttgattttc taagaaacgt
     3781 tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa cgacaaatgt aggtggtttg
     3841 acagcgactt gtgtagcata aaaaatcttc aggcgttttc ctttatctgt tggtgttgga
     3901 ttgattgcta cagcatccat aatgatatca ttcaacacag ccgaagagat acgaagattc
     3961 tggttcatac ttacttgttc gatcatttca ggcagacgtt ccaaccgttg tttagtcact
     4021 gcagaaacaa aaacgattgg agcgtaatca agataccgga attcatcacg aatctcttgt
     4081 tcgaaatcac gcattgtatt ggtttctttc tttacaagat cccatttatt cacgacgata
     4141 ataattccac gaccagcttc atgggcgtaa cccgctactc gcttgtcttg ttcccgaatc
     4201 ccttcttcag cattcagtac catcaaaacg atatcagaac ggtcaatggc acgcattgca
     4261 cgcatcacgc tgtatttttc agtattttcg tatacttttc cgcgtttacg cataccagct
     4321 gtatctatca ttaagaattt ctgaccactt tcagaaacga agtgggtgtc gattgcgtct
     4381 cgggtcgttc cttcaatatc cgaaacaatt acacgttctt ctcctaaaat cgcattgatc
     4441 aaagaggatt taccaacatt tgggcgacca atcaaactaa acttaatcgt gtcgtcgtcc
     4501 tcttcttcag tctcttcaga gaaatacttc actgcttcat ctaaaatatc accaatccct
     4561 agtccatggc ttcctgaaac aggataagga tcgcctagcc ctaaggaata aaattcatag
     4621 atatcgtttc tcatttctgg attatctact ttattgactg ctaaaatcac tggtttatta
     4681 cttctgtata aaattttcgc aaccatctca tctgcgtctg tcacgccttc gcgtccgctg
     4741 actacacaaa tgattacatc tgcttcttca atcgcgattt ctgcttgatg cttgatctga
     4801 tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt cgatcacgct gaattctcga
     4861 cctaaccatt caccttttgc ataaatacga tcacgagtta ctccaggagt atcttcgaca
     4921 atcgagatac gctcaccagc gatgcggtta aaaatcgtag acttaccgac gttcgggcgg
     4981 ccaacgattg caattgttgg atttgccata ttatttcctc ctcacaagtt tccaatctca
     5041 cttagtttaa cgaatgcatt gccaactttc aagcatagat tgtcaattaa acgaagtatt
     5101 tttattcctt attgaaatac taatcgcttt tcaaaagaaa aactgagaca aaagcagctg
     5161 ctttcatctc agtcacagat acaaaatcaa ttatttttca gaatcatcgg ttgctttttc
     5221 ttctacttct tctttcaacg catcacctaa aatgtctccc atagtaaatc cagtattttc
     5281 ttctggtaat tcataagact caacattttt tggttcttct tctgaagctg gtttttcttc
     5341 taaagctttg atactcaatg caatacgatg ttcttctgga tggatctcta atactttcac
     5401 tttcacttcg tcaccttcat gaagcacttc atgtggtgtc gcaatatgtt tatgtgaaat
     5461 ttgagagata tggactagcc cttctactcc agggaacact tcaacaaagg caccgaagct
     5521 tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact ggagcttttg tatcgatttc
     5581 tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa acacgttctt gttcaggatt
     5641 gatagacaac actttgacat ttacatcatc attgacagcc aatacatcac ttggttttgc
     5701 tacatgacta tgtgaaatct ctgagacatg gactagaccg tcaattccgc ctaggtcgac
     5761 aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg agaacatctc cctcatggat
     5821 agttgaaaga atttcttttt tcttttcttc tctttcgttt tccactaccg ctttatgcga
     5881 taagatcaaa cgattttctg atggttcgat ttcgatgatt ttaaagctca atgttttgcc
     5941 tttatagtct gaaaagtctg caacaaaatg atcttccacc attgatgcag ggacaaaacc
     6001 acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca tttgtaacag gtgcttcgat
     6061 gaccttgccc tcttggaaat ctctttcgat atcttcccaa acttttttag catctaaacg
     6121 gcgttttgaa agtagatagc tgccgttttc tttatctttg ccgatcgtac tgataacgac
     6181 taaatctaat acatcaccga cttttactac ttcgctgata tcttctaccg gcaaagttga
     6241 taattctttt gccggaacga ctccttcaac tccagcccct tcgattccga caatcacttg
     6301 tttgtcttca atgactaaaa cttcgccctt aacaacatcg ccaactttta cttcttgtac
     6361 gcttttcatt gcgtcttcca ttgactggtt gttctcttga ttttgatcaa attctgtcat
     6421 aacacttgtc ctcctaacca acattctgcc gtaagtacgg tactgttttt agtttaattg
     6481 attattcaaa aaaaataaag cgatttcgtt cgttttcctt gaaaaaacaa ttgtttttca
     6541 ttttttttct taatttaaaa aagaagataa ccttttttca aaacaacagc ttttattgct
     6601 gcaacgactt ctggaatcga tttgccagtc gtatcgatac gaacagcatc ctctgcttgc
     6661 ttcagcggag agacgtccct tgttgaatca taatggtctc tttcctgtat ctctttcgtc
     6721 agtgtttcaa gatcagtcgg aattcctttt tcttggttct ctttgaaacg tctttctgca
     6781 cgctcactta cgcttgctac taaaaagatt ttcacttctg cattcggtaa tacagctgtc
     6841 ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc ctatttttcg ttggacagca
     6901 actaattctt ctctgacttt tccatgtgca gagacttcag aaacagcttt tgtgacatct
     6961 ggttgacgga tttctaaagt cacatctttt gatgctatga atacatgctg tccatcttct
     7021 tgttgcgaaa aagagatagg atactttttg atcagcgaaa ctagcccttc ttcatctgaa
     7081 aaagatacat tgttcgtaat agctaaataa gttactgcgc gatacatggc accggtatct
     7141 gtataaatat aattcaattc ttttgctaat atcttggcaa cggtactttt cccagatgaa
     7201 gcaggcccgt cgattgcaat actgatttgt gtcatgatat aactcctttt tctcagaaaa
     7261 aaagccgcaa agcgcggcta ttattattta attcttaatt cttgaccagg gtaaagcata
     7321 aagttgtttg gatctaagcc attcaattca aacaattgat cagtggtgat ccctgcacgc
     7381 tcagccactt gttttggacc ttcgcctgct tgaacagtcg ttgttgaacc ggctgcagct
     7441 gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat cccctgcttg ataagttgcc
     7501 tcacttgagg aagtcggtgt tgtctctgct gcaatactgc tttctgacgt tacacctgta
     7561 tcttgagaca cagtagattc ttccgcgatt gtactagatt ctactgttga agtctcagtt
     7621 gactcctctt ttgttgtact tgtagaactt tctactactg atgtcgaagt ttcagaagaa
     7681 gttgctgctg ttgcattgtt gcttgatcca tttctgatcc agaaataagc acctgctgga
     7741 atagcgatac ataagaccag taaaatcaaa aatagcgaaa gaaataatgt atttcctttc
     7801 ttttgttgtc tctgagaact tcttgatgaa acttcatcat ctgtatcgta gattggttgt
     7861 tcccatggtt cttgtgtttc gttattttct gtagattgat gtctatcttt tctgctcact
     7921 gttttacctc ctaaactcta tcgtgtgtat tataccatag gaaaaagtgt cttgtcctaa
     7981 aaaatcaatg ttccatgttt tttttaaata aatttagtaa aatcgttccc caatgaagtt
     8041 gttcagtctt agaaaaaagc ttcctctttg gcagaagttc tgcttttgct ccatcaatat
     8101 cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa ataagctaag atgaattttc
     8161 tgcggcagtt ttcttcatta atatagcgaa gcatctgctg caggcggaat tctttttctt
     8221 tttcctgacg tttgagccgt tccacccaat tttctggttt ttcagactgc tggatcagct
     8281 ccatccattt tttttgcagc tcatcaaagg gagcttgctc tgtggcatat tccaaataaa
     8341 gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa gaaataatgg atacgttcat
     8401 ccccactttg ataaaaaaga attgctgcac tttcttcctg atctctgcct gctcgaccga
     8461 tttcttgtac atagttttct agactgtctg gcaaatcata atggaccaca taacggatat
     8521 cagatttatc gatccccata ccaaatgcat tcgtagcaat gagaaactgc agctgatttt
     8581 ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc aagaccaccg tgatagtatc
     8641 caactgtaaa acgttccctg aataaatgat acaatcgttc gacttctttt tttgttgcac
     8701 aataaatgat tgccgctcct tgtgctcttt ccatgaagtg ttcaagttct tgttcttttt
     8761 gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt gactgattgt ctgatctctt
     8821 ttggtggtcg atcaaatagt acatgacgta tatctcttgc tactaaatct gttgctgtcg
     8881 ccgttagtgc cagtgtgacc gggtttccta aaagctttcg gatttttcct aactgttgat
     8941 attctggacg gaaatcaact ccccattgag acacgcaatg agcctcatct actacatata
     9001 acgcaatatt ttgttttttc agatgctcta aaagagaagg attagtcaac atttcgggac
     9061 tcaaaaacaa aaatttatat tgcgatagat gacggaggac gtatcttctt tcagattgcg
     9121 aaagtaaact attgaaagca gctactcgtt tctcccctcg tttttgtaat tgcgtcactt
     9181 gatcttccat tagagaaagc agcggtgaca cgatgatgac cagaccttcc attaaatacc
     9241 ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg caaaatagcg agagtatctt
     9301 ctccatcaag caacgcctga ataatttctt cttggccggg acgaaatgaa tcaaatccaa
     9361 aatatttttt taattcattt attaacattc tgtatcgctt cctttctcaa tagatagatt
     9421 tgatacaaac gaaattctcc ataatctaat tgtccatcta cattccattc gctaaagcgc
     9481 cactctcgca cggaatcctt atttggttcc aaacgttcga ttgtttctgc agagagaatc
     9541 cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa taagatgatc tgttactgtt
     9601 cctcttttta aatgacggat tgccatgatc tggtcaattg tctgaccatt cataaacatc
     9661 tgcttggtga tcatcatgct ttgattgaaa ttttgctgta ataatgaatc gatcaaagcg
     9721 tgccaaagcg aatctggccg tttttcgatt tgtgctaaca aaaggtggat acatctgctt
     9781 tgttgcagat aaaccgctga ctcatcattg ttttttgcca gctgataagg aagcagccct
     9841 gttcgctcat ttccagataa ttggttagct aaaaaatctg ctgcttcttc tggtatctcc
     9901 gaaaacagct gcaccaaact ctcatacgca gtatcaatca aaatttcacg tggcaattgg
     9961 ctttcactaa gccatttttt tagttggaaa gtataaaaag gacttgtctc tgctggaaga
    10021 tagtcttgaa tacccgttgc cagattagaa attacttgaa cagcaaattt aatgagccgc
    10081 caactagttt cccctgttct gccatagcgg tcaaaacgca aaccagtatg ttctatttga
    10141 tgttcggaca acatatcagc ccctgcaggc gttagctttg cttcattttc atttatttcg
    10201 atccaacctt gctgcgcaat tttttgcaaa atttgataaa acttatcctg cttcaattct
    10261 ggaaaactgt tatggataaa caaaagttcg tgggtaaatc cataaataag tactgaagtc
    10321 gttcgttttc ccacaaggag ttggtaaaga gaactaatcc gcagcttgtg tcctgatgaa
    10381 aatagcgata aaataaaaac aatcatatta ttctccttaa gttaggatgg ttagaaatgt
    10441 cactactatg taaacttgta cctgaacgct gtattgcatg cggtctttgt caaatcgaag
    10501 cgccagatat tttcgattat gatgaagaag gaatcgtttt atttgcccaa gaaccagagg
    10561 ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt aaaaaaagcg tatcaaaaat
    10621 gtccagttcg agcgatttta ttagaaaata aaatataaat ataaacgaaa ctttaaaaac
    10681 gtacactaag ttctctaaac ttgaaaataa gccgggatct gtttctcttg ttccgtgaaa
    10741 tcccgactta ttttgaaaga ttcatttgct atctatagtt aattgactag ttttttcacg
    10801 gaccctatag gacccattta tattatttca actttttcag ccagttttcc aatgtattga
    10861 cgatcacagt ttgctggtct gcgttagaaa tttctgcctc tccatctcct ttttgctcac
    10921 catagctgcc aaaacctcca tgatttccgc cattgattga tacatactct gttctttccg
    10981 gtaaatactg cttagcttct tgataagctt cctgattcaa tactttatct tttgtcgccg
    11041 tgatcgataa aacaggaaca gctgattctg tcaatgttcc ttttttgtcg ggataacttg
    11101 ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt cttattgaca taacgacttg
    11161 ccattacgcc acctaatgaa tggccaccga tcacataatc ggaatttgga aaatcttctt
    11221 tgaccttatc cgctttgttt ccgcccatca ctgctagatc aagtggcatt ttgacaatag
    11281 cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat actataactt tctggctcta
    11341 cgagagcacc aggataaaaa ataagtaatg gagactttcc atgattcttt ttctctcctt
    11401 caaaaaataa gtaatcttct ttgttttttc cttcttttac caacacttgc gcttctgacg
    11461 atggttcgta cgtattttgc tgtatatagt aataaccggc tccaccgagt aatcctaaaa
    11521 tgatgactaa gccaattagt attcgtctaa atattttcac tttctcccac cttttctatc
    11581 tgagtgaccc ctttttctta atcaagtatg attatacctt aaaaaaataa gagagaaatg
    11641 caaactttcc agcatcaaat aaaaaaagac ggaatatcca tttgaatatc cgcccttttt
    11701 gtatgtttat ttttatttag cacataaacg atacacagct tctgcataaa tttccattgc
    11761 tttatacata ctacttaatt cccatcgttc attcgcctgg tgcataaagt caggagtatc
    11821 agggaacatc gcaccgaatg ccacacactg gttcatcgta cgagcaaaag tagcgccgcc
    11881 tgatacaaac ggttcagtca tgtctccagt aatatcgcga tatgtcccca atagattttt
    11941 cactaactca ctgtctagtg gcacgtataa tgaatcaagg aagtcaaatt cttcataagt
    12001 caacccatat ttttctgctg ttttagttaa tttagctact aaatcatctt tcttgaatgt
    12061 aacagggatt ctcatatcta caccgatttt tgtttgttct ggtgtgattt ccaaacttgc
    12121 aaagttcatc gttagctcgc cggattgttc atcttctgtt ttgcccacga cagcttcacc
    12181 agttgcattt tcttgaacta attttcctaa gaaattgatt gggccaaaat caaatacttc
    12241 tgaaagagca attgccagac gagaaatagc attcactcct tgtgctgcat ctttcgcgtg
    12301 gatactttta ccaagaacga cgatgccttc accttgttct tcgaaatcga attcgtgttt
    12361 tttcaatgct tcttttactt cagaaagctt ttctccagag taaggagctg catcaggaac
    12421 aacattcaac gctcctccag cttttacaga aaactcgttc gttcctggtc ctgtcaagta
    12481 agcttgcaat aagccttttt ctgcataaat taaaggaaat tcagcatctg gcgcaaatcc
    12541 ttgcgtaatt ccttcttctt tttcgttata tttttctaaa caacgccata aattttcttc
    12601 atctgtacca aagataaaac gaatacgtgt cttgaattcc acgccagcat caatcaaagc
    12661 tttgacggca tacattgccg caatagaagg acctttgtca tcttgggatc cgcgaccaac
    12721 taaccagccg tcttttatag ttggatcgaa tggttttgtt tcccaattat tttcgtcccc
    12781 tgctggaaca acgtccatat gacacaagat accgaataat tcatctccag agccaatctc
    12841 tgaataaccg taatagcctt ctggatcttt aaatgttcta aatcctagct tttcactgat
    12901 ttccaatact ttatccaaag cttcaatcac ttttttaccg aagggatgtc cttgtcctga
    12961 atctgcttca tctagtacgg aaggaattcg gatcaattcg ctcaatgatt ctaatgcttg
    13021 ttcatgatgt tgttctgtca caaatttttt catgatatta tcctcctaat atgttaaaaa
    13081 gggtcatgtc aaattcctcc gacacaacct cttcttctca aataatgacg ttttaagcct
    13141 gctccatcag caagcagaat gttcttaaca aatgatgaaa gaaaaaacac tcactgaatg
    13201 gagctattga cttatccacg tgcttatcga tagccgttta tcggtttctt cattcttatg
    13261 atagaccttg tacctgtatt tgtctaggtt tagaacattg ctgctacacc taggatcaca
    13321 cagatggcta caaataatat cgcaattaat ttaccggtaa atttccacca cactgaaaca
    13381 tcgaaacgag caattgctac agcacccatt acgatagcag atgttggtgt aattaagttt
    13441 actaaaccag atgcagattg ataagcagtg attactaaat ccttaccgac gccggcaaat
    13501 tcacccatag gtcccatgat tcccattgtt gcagcagcta agccggaagt tgaaggaatc
    13561 aagaatgaca tagggatata gaaaataaat gtaataatga tgaatacact tgaagataat
    13621 gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg tgattaatcc attgttcata
    13681 accacttgga taccacgagc aacagctact acgattgcta caccgatcaa atcagaagca
    13741 ccagctaaaa attcagagac aaattcagac tctttcatgc cataagtgat tgctactacg
    13801 atggacatta ctaagaataa catagtaatt tctgggaagt accagtcacc taatggaagc
    13861 atgcttttac ctagaaccgt acctagtacc ggaaggtttg ttaaccagtt tgtcaatgat
    13921 tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa tcaaactgat gatcatgata
    13981 ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt ttttcgtgat tacttcttga
    14041 cgaccgattg cttcaatatc gaaatgcttc atatcttctt tacgttggtt gtatactaat
    14101 gattttgttg gatctttttc gatctttgat gcataacgat acacatatac aatagaaata
    14161 gctaacaaaa taacgaacat caagaatcga agaccaatac cttcacccat cgaaatccca
    14221 acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg ttgaagctag acagcctact
    14281 tgagacccta ctaatacgat tgctacagct actactgtat caaagcctac agccatcatg
    14341 actgggatca gtaaagcata gaatggaatc gtttcttctg ccattccgta agtagagccg
    14401 cctaatgcaa ataacagcat caatactgga ataagcattt tttcttttcc tttgaatttc
    14461 ttcacaactg aagcaatccc ggcatctagt gcccctgttt tgttgataat acctaaaaat
    14521 ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag caggtgtttc tttagttagc
    14581 tcccccgctg gtacacctag catcccattg attggcgcca tgaaaatatc ccacagccct
    14641 tgaggatttt gttgtactcg ttcatatgat ccagcgacga tgttgcctgc atcgtctaca
    14701 ctatagtgcc cagctggtat aaaccaagta agtacagcga ttactgcaat aataataaat
    14761 aagaccgtat acgctgaagg catttgaaat tttttctttt ttgtttgttc catcttcttc
    14821 ttcctttctt ttccgttaga gacaacaatt ttggctctaa tgttatttta tcaaaaagag
    14881 tatcagaaca aaaataataa cacgcatttt gtaaaacttt tgttcaaaaa gttcgttttt
    14941 ccctattccc tcctcttttt tcaggttttt aaagctttct tctttttcat tgtagcgttt
    15001 cctttttttt ataaacaaac attttcgtct tttaaaagga gaagaat
//
LOCUS       DAZULG010000067        14428 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000067 DAZULG010000000
VERSION     DAZULG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.069"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1115)
                     /locus_tag="ACG9CY_001863"
     CDS             complement(228..1115)
                     /locus_tag="ACG9CY_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4938365.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="ACG9CY_001864"
     CDS             complement(1112..1981)
                     /locus_tag="ACG9CY_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4938366.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="ACG9CY_001865"
     CDS             complement(2118..3404)
                     /locus_tag="ACG9CY_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4938367.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="ACG9CY_001866"
     CDS             3677..4255
                     /locus_tag="ACG9CY_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4938368.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="ACG9CY_001867"
     CDS             4255..5184
                     /locus_tag="ACG9CY_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938369.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="ACG9CY_001868"
     CDS             5181..6917
                     /locus_tag="ACG9CY_001868"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4938370.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACG9CY_001869"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACG9CY_001869"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS4938371.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="ACG9CY_001870"
     CDS             complement(7813..8322)
                     /locus_tag="ACG9CY_001870"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS4938372.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACG9CY_001871"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACG9CY_001871"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS4938373.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="ACG9CY_001872"
     CDS             complement(9623..10714)
                     /locus_tag="ACG9CY_001872"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS4938374.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACG9CY_001873"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACG9CY_001873"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS4938375.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACG9CY_001874"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACG9CY_001874"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS4938376.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACG9CY_001875"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACG9CY_001875"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS4938377.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4108 a         3141 c         2608 g         4571 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgc
//
LOCUS       DAZULG010000068        14426 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000068 DAZULG010000000
VERSION     DAZULG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.070"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..825)
                     /locus_tag="ACG9CY_001876"
     CDS             complement(130..825)
                     /locus_tag="ACG9CY_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS4938378.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="ACG9CY_001877"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="ACG9CY_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS4938379.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="ACG9CY_001878"
     CDS             complement(2024..2215)
                     /locus_tag="ACG9CY_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS4938380.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="ACG9CY_001879"
     CDS             complement(2236..3126)
                     /locus_tag="ACG9CY_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS4938381.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="ACG9CY_001880"
     CDS             complement(3113..3880)
                     /locus_tag="ACG9CY_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4938382.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="ACG9CY_001881"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="ACG9CY_001881"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS4938383.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="ACG9CY_001882"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="ACG9CY_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS4938384.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="ACG9CY_001883"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="ACG9CY_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS4938385.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8451..8834
                     /locus_tag="ACG9CY_001884"
     CDS             8451..8834
                     /locus_tag="ACG9CY_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938386.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(9002..9760)
                     /gene="jag"
                     /locus_tag="ACG9CY_001885"
     CDS             complement(9002..9760)
                     /gene="jag"
                     /locus_tag="ACG9CY_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS4938387.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="ACG9CY_001886"
     CDS             complement(9778..10596)
                     /locus_tag="ACG9CY_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS4938388.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="ACG9CY_001887"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="ACG9CY_001887"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4938389.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="ACG9CY_001888"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="ACG9CY_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS4938390.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11725..13059
                     /gene="dnaA"
                     /locus_tag="ACG9CY_001889"
     CDS             11725..13059
                     /gene="dnaA"
                     /locus_tag="ACG9CY_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS4938391.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13257..14387
                     /gene="dnaN"
                     /locus_tag="ACG9CY_001890"
     CDS             13257..14387
                     /gene="dnaN"
                     /locus_tag="ACG9CY_001890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS4938392.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         3992 a         3042 c         2586 g         4806 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggagtccta tgtggaaaaa gttatgcaca gggattatcc acagataata aatggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggtgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctatat tttttatcca cagatgcaag aggaaatcta
    11521 ttgattttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt ttttgacaac
    11581 tgtgtttttc tcttttttct ttttgtcgat tgtgtggata attgtaatta aaaaattttt
    11641 ttcttcggtt tctgtggaaa actattgaaa aaaatgctaa attagtacta gttattcact
    11701 ttttaatgaa ggaggaggcc ttatatggta tccctcgatg ctttatggaa tgaattaaaa
    11761 gcaacatacc aaaaggattt atcgcctgct agctataata catggatcga aacagcgaac
    11821 cctcgaaccc ttgatcaaaa ccagctcgtt gttgaggttc ctagcaagat tcataaagaa
    11881 tattgggaaa aaaacctggc gacaaaaatc gttgaagtcg gttatatgtt atcaggaaat
    11941 gagattatcc ctcgctttat tactggcgaa gaagcagagc aagaagaagt tatagaagaa
    12001 aaaaatccaa aagttgtggc acctagtccg ctaaaaaaag ccatgctgaa ccctaaatat
    12061 acctttgata cgtttgtcat tggtaaagga aaccagatgg ctcatgcagc agcgctcgtt
    12121 gtagcagaag atccaggttc tatttataac ccgctcttct tttatggagg cgtgggactt
    12181 ggtaagactc acttgatgca tgcaattggt catcaaatgc tgcaaagtca gcctaatgcc
    12241 aaagtaaaat atgtaagcag tgaaactttt gctaatgatt tcatcaattc gatccaaaac
    12301 aaaacagcag aagagttccg tcaagaatat cgaaatgttg atttactgct agtggatgac
    12361 attcaatttt ttgcagaaaa agaagctacg caagaagagt ttttccatac tttcaatgcc
    12421 ttgtacaatg aagggaaaca aatcgtacta accagtgatc ggttgccaaa cgagattccg
    12481 aaactgcaag aacgcctcgt ttctcgcttt gcatgggggc tgtctgttga tattacaccg
    12541 ccggatcttg aaacaagaac agcgatttta cgtaaaaaag cggcggctga acgtctagag
    12601 atcccagatg acacactaag ctacatcgca ggacaaattg attctaacat tcgtgaatta
    12661 gaaggtgcgc ttgttcgagt gcaagctttt gctgcgatga atagtgaaga tatttcgact
    12721 agtctagctg cagacgcact gaaaacgtta aaatcaggaa aaggacatcc tcaattgtca
    12781 atcctgcaga tccaagaaga agtggcaaag tattatcaca tccaattgaa agatatcaaa
    12841 ggaaagaaac gcgtcaaatc tattgttgtt cctagacaaa tcgctatgta tttggcacgt
    12901 gagctcacag ataattcttt accgaaaatc ggagctgaat tcggtggcaa ggatcataca
    12961 acggttatcc atgctcatga aaaaatccag cagctgatgg attctagtgt cagtatgcaa
    13021 aatgaagtat cagaaatcaa aaacttttta ctaaattaat ttgtcgattc tatcaactga
    13081 aaatagcatg tggataaaag aaaaaaacgt tcaaaagtta tgcacacttt ttccacagct
    13141 gttttttcgt ttctctctta cgttttttga gttttccaca gaatcaacat gccctattac
    13201 ttttattatt ctttttaact aataataaat aaacaaacat tcaaaggaga tacatcatga
    13261 aagttacttt aaaccgagct agctttatgc aggaattgca aactgttcaa cgagctattt
    13321 caagcaaaac cacgatccct attttgacag gtgtaaaaat cacactgaca caagaaggtt
    13381 tgactttgac ggggagcaac gctgatatat caattgaaac ttttttgtct gttgaaaacg
    13441 aaaaagcaaa tatgcaaatc gaatctactg gttccattgt tttacaagca cgtttcttta
    13501 gcgaaatcat tcggagactt cctgaagaaa catttacttt agaagtttta gaaaataaac
    13561 aagtagcgat tacttctgga aaagcgaatt ttatcgtaaa tggattagat gcagataact
    13621 atcctcatct tcctgttgtc gaaagccata accagatgaa attacctgta cacgtattga
    13681 ctaaactaat caacgaaaca gtttttgctg tctcacaaca tgagagtcgt ccaatcttga
    13741 caggtgtgca tttcatttta tctgataatt ctttattggc tgtagctact gattctcacc
    13801 gtctaagtca acgcgtgatt ccagtagaac aagcggctga tcattttgat attgttattc
    13861 ctggaaaaag tttgatcgaa ttatctcgct cattaacaaa tgaagaagaa atcgtcgaaa
    13921 tcagcattat ggaaaaccaa gtgttgttca aaacagaaac gatgtatttc tattcccgtt
    13981 tgctagaagg aaactatcct gataccaatc gtttgattcc ttcaagtttt aatacagagg
    14041 ttgaattttc tgttcctagc tttttagcgg cgatcgaacg ggcttcttta ctttctcatg
    14101 aaggccgtaa caacatcgtt cgtttatcta ttcgtccaga tgcggttgtt ttatacggaa
    14161 attcgccaga aatcgggaaa gtagaagaaa gcttgagtta tacagcaagc agtggcgatc
    14221 cattagatat ttctttcaat ccggattata tgaaagcagc gttgcgtgca tttggagata
    14281 tgagcatcaa agtgaaattt atctctgcta ttcgtccatt tacattggaa ccaacggaag
    14341 atggcgttca atttattcag ctgattactc ctgtacgtac aaattaacaa tgattttcat
    14401 tgttcttgaa aatttaattg aaaaga
//
LOCUS       DAZULG010000069        14186 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000069 DAZULG010000000
VERSION     DAZULG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.071"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1345)
                     /locus_tag="ACG9CY_001891"
     CDS             complement(143..1345)
                     /locus_tag="ACG9CY_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4938393.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="ACG9CY_001892"
     CDS             complement(1348..1542)
                     /locus_tag="ACG9CY_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4938394.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="ACG9CY_001893"
     CDS             complement(1589..2437)
                     /locus_tag="ACG9CY_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFS4938395.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="ACG9CY_001894"
     CDS             complement(2642..2992)
                     /locus_tag="ACG9CY_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938396.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3170..4033
                     /locus_tag="ACG9CY_001895"
     CDS             3170..4033
                     /locus_tag="ACG9CY_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4938397.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="ACG9CY_001896"
     CDS             4657..5649
                     /locus_tag="ACG9CY_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4938398.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="ACG9CY_001897"
     CDS             5665..6549
                     /locus_tag="ACG9CY_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4938399.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="ACG9CY_001898"
     CDS             6598..8205
                     /locus_tag="ACG9CY_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4938400.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="ACG9CY_001899"
     CDS             8206..9690
                     /locus_tag="ACG9CY_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS4938401.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="ACG9CY_001900"
     CDS             9867..10859
                     /locus_tag="ACG9CY_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4938402.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="ACG9CY_001901"
     CDS             10911..11780
                     /locus_tag="ACG9CY_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4938403.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..13813)
                     /gene="guaA"
                     /locus_tag="ACG9CY_001902"
     CDS             complement(12200..13813)
                     /gene="guaA"
                     /locus_tag="ACG9CY_001902"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4938404.1"
                     /translation="MRRTERAAYRTKFYSNLSSPTRFDEEPKIIVLDYGSQYNQLITR
                     RIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVL
                     GICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVT
                     QAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTG
                     DWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVL
                     LRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDE
                     ATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLF
                     KDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANA
                     GLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKI
                     SVRIVNEVAHVNRIVYDITSKPPATVEWE"
     gene            complement(13820..>14186)
                     /locus_tag="ACG9CY_001903"
     CDS             complement(13820..>14186)
                     /locus_tag="ACG9CY_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4938405.1"
                     /translation="KRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQE
                     TLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYR
                     NFYNFRARIYLQQGLIFEN"
BASE COUNT         4342 a         2405 c         2927 g         4512 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tttggctctt
    13741 cgtcaaatcg tgttggtgaa gacaaatttg aataaaactt agtccgatat gcggcgcgtt
    13801 ctgtgcgccg catatttttt tagttttcaa agatcagacc ttgttgtaaa tagatccgtg
    13861 cccgaaagtt ataaaagttg cgataaccaa acgcaattcg tttaatcagc ttgatcttgt
    13921 tattcagccc ctcagtgacg ccgtttgagt gggacacctt aaaggcattt gaaatacccg
    13981 attggtagcg atggaagatc gtgaatttct ttttaaacga ctgaggtaac gtagatgaaa
    14041 ttgttttaag ggtttcttga aagccttccg tacctcgttt aacgaaataa tagcgtaata
    14101 attgacaaaa atgatacgca gttcgcaatt cttcgttgta gcttaacagc tcatcaacga
    14161 tttctgtttg actaattggt cgttta
//
LOCUS       DAZULG010000070        14129 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000070 DAZULG010000000
VERSION     DAZULG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.072"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG9CY_001904"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG9CY_001904"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS4938406.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG9CY_001905"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG9CY_001905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4938407.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACG9CY_001906"
     CDS             8118..9086
                     /locus_tag="ACG9CY_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4938408.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACG9CY_001907"
     CDS             9104..10009
                     /locus_tag="ACG9CY_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4938409.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACG9CY_001908"
     CDS             10006..10812
                     /locus_tag="ACG9CY_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938410.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACG9CY_001909"
     CDS             10969..11937
                     /locus_tag="ACG9CY_001909"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS4938411.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACG9CY_001910"
     CDS             complement(12254..12778)
                     /locus_tag="ACG9CY_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS4938412.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14129
                     /gene="scm"
                     /locus_tag="ACG9CY_001911"
     CDS             13065..>14129
                     /gene="scm"
                     /locus_tag="ACG9CY_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4938413.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4001 a         3048 c         2787 g         4293 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tcttttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 catcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggcgaa aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggagtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 atgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatca
//
LOCUS       DAZULG010000071        13959 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000071 DAZULG010000000
VERSION     DAZULG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.073"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACG9CY_001912"
     CDS             <1..135
                     /locus_tag="ACG9CY_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4938414.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG9CY_001913"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG9CY_001913"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS4938415.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="ACG9CY_001914"
     CDS             complement(2412..2663)
                     /locus_tag="ACG9CY_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS4938416.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="ACG9CY_001915"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="ACG9CY_001915"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS4938417.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="ACG9CY_001916"
     CDS             3613..3831
                     /locus_tag="ACG9CY_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938418.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="ACG9CY_001917"
     CDS             complement(4120..4632)
                     /locus_tag="ACG9CY_001917"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS4938419.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACG9CY_001918"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACG9CY_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS4938420.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="ACG9CY_001919"
     CDS             complement(7085..7528)
                     /locus_tag="ACG9CY_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS4938421.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="ACG9CY_001920"
     CDS             complement(7529..8317)
                     /locus_tag="ACG9CY_001920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4938422.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACG9CY_001921"
     CDS             complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACG9CY_001921"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS4938423.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="ACG9CY_001922"
     CDS             complement(9432..10019)
                     /locus_tag="ACG9CY_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS4938424.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACG9CY_001923"
     CDS             complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACG9CY_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS4938425.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="ACG9CY_001924"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="ACG9CY_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4938426.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="ACG9CY_001925"
     CDS             complement(12936..13871)
                     /locus_tag="ACG9CY_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4938427.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3863 a         3126 c         2454 g         4516 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAZULG010000072        13926 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000072 DAZULG010000000
VERSION     DAZULG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.074"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..910)
                     /locus_tag="ACG9CY_001926"
     CDS             complement(35..910)
                     /locus_tag="ACG9CY_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4938428.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1058..1390
                     /locus_tag="ACG9CY_001927"
     CDS             1058..1390
                     /locus_tag="ACG9CY_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4938429.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1502..2710
                     /locus_tag="ACG9CY_001928"
     CDS             1502..2710
                     /locus_tag="ACG9CY_001928"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS4938430.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2864..3319
                     /locus_tag="ACG9CY_001929"
     CDS             2864..3319
                     /locus_tag="ACG9CY_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938431.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3681..3956
                     /locus_tag="ACG9CY_001930"
     CDS             3681..3956
                     /locus_tag="ACG9CY_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938432.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3969..6047
                     /locus_tag="ACG9CY_001931"
     CDS             3969..6047
                     /locus_tag="ACG9CY_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4938433.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            6210..8093
                     /locus_tag="ACG9CY_001932"
     CDS             6210..8093
                     /locus_tag="ACG9CY_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4938434.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            8105..9052
                     /locus_tag="ACG9CY_001933"
     CDS             8105..9052
                     /locus_tag="ACG9CY_001933"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS4938435.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            9072..9599
                     /locus_tag="ACG9CY_001934"
     CDS             9072..9599
                     /locus_tag="ACG9CY_001934"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4938436.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(9662..10315)
                     /locus_tag="ACG9CY_001935"
     CDS             complement(9662..10315)
                     /locus_tag="ACG9CY_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS4938437.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            10447..11289
                     /locus_tag="ACG9CY_001936"
     CDS             10447..11289
                     /locus_tag="ACG9CY_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4938438.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11447..12343
                     /locus_tag="ACG9CY_001937"
     CDS             11447..12343
                     /locus_tag="ACG9CY_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4938439.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            12346..13059
                     /locus_tag="ACG9CY_001938"
     CDS             12346..13059
                     /locus_tag="ACG9CY_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS4938440.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            13079..13597
                     /gene="msrA"
                     /locus_tag="ACG9CY_001939"
     CDS             13079..13597
                     /gene="msrA"
                     /locus_tag="ACG9CY_001939"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4938441.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            13594..13821
                     /locus_tag="ACG9CY_001940"
     CDS             13594..13821
                     /locus_tag="ACG9CY_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS4938442.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
BASE COUNT         4800 a         2398 c         2874 g         3854 t
ORIGIN      
        1 aatctttcct catagcctat ctttgtcttt tttcttatag ttttttcagt aatcttgtct
       61 gtggcttcaa ataagtaaag ccttgccctt ccacttcatg cacattgatg attgaaagga
      121 aagctttttc gtcaatctcg tggacgatcc tcttagcttc agttaattca ctagagctaa
      181 cgaccatata gatgattttt tttgaattac cagagaatgc gccttctcca gaaaggaaag
      241 tcacacctct ttgcagaccg gtcatcagta gtggtgcaat ctcttcgctt ttgttggaga
      301 cgacaagtag accttttgca gaatatccgc catctaagat cgaatcaatc acacgactga
      361 aaacatacga aacaatcaac gtatacatca tacgtttcaa gtcgatatag gttaatgaag
      421 caagtaagac gatgatatca aatgccaata gggaacgccc catagtgatg ccgtaatttt
      481 gttctaaaat ccgagcgata atatcactgc cgcctgttgt tccacccatg cggtagacga
      541 tcccgcttcc tacacctgca actaaacccg caagtagaga aacgatcagt agatcatgct
      601 gtaaattgat ttctagtgga accctttgcc agatccataa aaagaatgac aaagaaagtg
      661 tacctagaac agtataggcc aaggaacgtt taccgaggat ctttcctcct agtaaaatca
      721 atgggatgtt gattaataaa gtcgtataag ctggatcaat accaaataaa gctcttaaga
      781 tcaaggtgat ccctgagact cccccttctg ctaaatgatt agcgatgttc aagtaaacaa
      841 gaccaaatgc ataaatactt gttcctataa tgataaaaag aacttctttt actgtttttc
      901 gtttgaccat atgaattcca gctttcaatt atgtattttc acagaatttc catttagaaa
      961 ttccttccta aatgtagcat ggaaattcga ttttctcaat tgctcttatc taggatttgc
     1021 gttaaagttt tggtaagata gttttggagg gaaaaagatg gataaaaagc gttctttata
     1081 cagtatgcag caggaagtgg atgaatatat ccagcaattc aaggtggggt atttctctcc
     1141 attagctcag atggctcgat taactgaaga agtaggtgaa ttggcaagag aagtcaacca
     1201 cacttatgga gaaaaaagca aaaaggcttc tgaaccagtt aactcggtag cagaagaact
     1261 tggtgatgtt ttattcgtta cgatgatcat ggctaattca ttgaatatcg acttaactga
     1321 agtttttgaa aaaaacatgg aaaaattcaa tcaaagagac cataatcgct ttgagcgtaa
     1381 ggacgagtaa acaagatgaa gtggtcctgg ccttgaagac tctgaaacgg acttgtcaaa
     1441 cagtcaccat gcaactgagt ctttatctca ggacaagaaa cacgcaggaa aggttggaaa
     1501 aatgaaatta aaacaattac ctctggaata ccaaaaggca attccagttc tgaaaaaatt
     1561 agagaatgca ggatatgaag cttattttgt aggtggaagt gttcgtgata tcttgctgaa
     1621 ccagccaatc catgatgtgg atatcgcaac aagcgcattt ccagaagaaa tcaaacagct
     1681 ttttccaaaa acgatagata ttgggatcga acatggaact gtgttggtat tagaaaatga
     1741 tgaacaatat gagatcacca catttcggac ggaatcaact tatcaggact accgtagacc
     1801 agatcatgtc gaattcgttc gttcgttggc agaagatttg aaaagaagag attttacgat
     1861 caatgcgttt gctttgaaag aagatggaga aatcatcgat ttgttcgatg gtctgcaaga
     1921 tctagaagaa cagatcttgc gagctgtagg caatcctcat gagcggttcc atgaagatgc
     1981 cttacgaatg atgcgcgggt tgcgttttgt cagtcaacta gggttcaaat tagaggaaga
     2041 aaccttctac gccatcaaag aaaaccacga gctattaggt aaaatatctg tagaacgtat
     2101 aaatgtggaa ttcatcaaga tgctcttggg aaatgaacgg gccgcgggaa tccatgcatt
     2161 tattgaaaca gagtgctaca tttgctgtcc cggtttgaaa gaagaaggcg aagcactttt
     2221 acgttttgct gaattaccat ccgtcccatt gaaagaagag agccaagcat gggcattatt
     2281 gatcagccaa ctggatctaa aagaaaatga gattcgttcc tttttgaaat cgtggaagtg
     2341 ttctaaccaa atgattaaag acgttcaagc acttgtccaa ggactacgac aacgagaaac
     2401 tggagctttg gaaccattcg aattgtactt cttaggcaaa aaacatgcac tgcaagtaga
     2461 agaattgatg atctattttg acgaaacacc tcagatggat gccgtaggag aatcctatga
     2521 tcgtttacct atcaagtcat taagcgaatt agcagtagat ggtaaaatct tgctagaaga
     2581 aactggaaaa aaacctggta agtgggttag tgaagctttg caattagctg aaaaagccgt
     2641 cgtagaacaa agaataaaaa atgataaaaa acaggttttg gattttttat cgaaagaaaa
     2701 attgatttaa aaaacactag actgtatctg ctcagctttg ttggctactt aaagtgggca
     2761 gatacgtcat tagaagaaaa aacataagat gtgaccactt acataaattt gtgctaaact
     2821 gaggtagtac aagaataaat tacatcataa aggagtcttt ttaatgagta aagaaatgac
     2881 agcgctaaaa ttttatttta gaaatggaga aacctggacg atcaaccgcc gtcatatcgg
     2941 cgatctttgg atcaaacaaa tcacaacaag ttttggacga atcaatggca gtgaatttgt
     3001 tgaaatccat ccgtgtgctg gatttaaaat cgaaatcttc catgaagggg acgcagttgc
     3061 gacacatgat atcaacctag gcggactgga aatgggcatg tttaatcgcg cattgaaata
     3121 cgaagatatc gagcgaatgg aaatccttta ccgaaatggc acacctgatc ttgtgtactt
     3181 cccatatcta gataaaggaa cagaaggatt agacaatcaa tatcaatcaa caaaaatcag
     3241 cgaaaaaaca ggtaacttgt atatcgtgat caatccagat caacgtgtag aagatgtata
     3301 cggggaattt ttcgaataaa tcgattttta cgcaaaaatg aggctgtgaa agagatgtgt
     3361 cttattacta ctgataggct gtctcttcca caccgtttat tcagaataag ggactgtgac
     3421 aagatttttg tcgcagcccc atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg
     3481 ggaaagtaaa catccttttg tcagaaactt ttatctttct taagttttac ttgatatctg
     3541 atactatttg gaatagaatg aggcgttgaa aaatttatgg aaatttctaa ctagcaataa
     3601 acgatgaaac aatgaaatat ttttcatcca aaaagaactt tgctataatg atggaagttt
     3661 gtgtatagga gatctggaaa atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg
     3721 atcgctatgg cgccctttcg atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa
     3781 gaaatatatt gatgtatcgt cagttggaac gtctgaaaca tgatctgctt cgtttggagt
     3841 caaaacgtgt acagtgtgaa ttagagggga accaaacaca ggttgaagta gtcgaaacca
     3901 aaattttaaa gaaaaaagaa caatttttaa aaatgcttac acaaaataaa aaataaaggg
     3961 gatggacgat ggaatttgct gagttagtta ttgtatttgc agtaacgatc accttatcga
     4021 acatagctag ccgcattttg ccaatgattc cagctccact gatccaaatt ttccttggag
     4081 tggtattagg tctgacagag tggggacaat caatcgattt tgaacctgaa ttgttcttag
     4141 ttatgattat tgcaccattg ttatttagag aaggagaaaa agcagatatt tcttcgattc
     4201 tcaaaaattt cgggacaatt ttgttcttgg catttggagg agtcattttg acccttgtcg
     4261 gggtaggtgc gacgttatca ttcctgttgc caagtgtccc acttgctgcc tgctttgctt
     4321 ttggagcagc gttaggtccg acagatgcgg tagcagtcag ttctctttct ggacgagtga
     4381 atatccctaa aaaggcgatg catattttgg aaggagaagg tcttttgaat gatgcatctg
     4441 gtgttactgc cttccagttt gctcttggtg cgctgatcac cggatctttt tcagcagtaa
     4501 acgcggggat gagtctagta gtttctagta ttgggggagc tttgatcggc tttttgcttg
     4561 tctggttcaa acaaaaaata attcatttga tcgaaaaagc atcagcacaa gatgtcacag
     4621 ggtatctgct gatcgaactt ttgcttcctt ttctagccta tgtattggca gaggcatttg
     4681 atgtatctgg aatcattgct gcagtggcag caggtattct ccaagcttct ggttttagga
     4741 aaatctctgt gtttgatgca gaattgtcga gcttgtccca cagtacatgg acaactattg
     4801 cttttacact gaatgcatta gtatttatat ttttaggaat cgaattgacg caagtgttct
     4861 cgccagtatg gggagacggt ttatatccaa acggtctttt attagcgatt atcgtattaa
     4921 tctctgtcat gctttttgtg atcagattca tttctatcag tcttttttat gtattcaaag
     4981 atggcagtaa aaagttcaaa aaacagttga acgagatctt gatcttaaca tttggcggtg
     5041 taaagggaac agtcagtttg gctacgattt ttattttgcc attttcaatc aataatatga
     5101 tgttttacca gcggtctttg cttttgtttc tgacggcggg tgtcatctta gtgactttgg
     5161 tcatagggat cattgtcttg cctatgctga cggaaacaga ggaagcgaaa agcacggatt
     5221 tgaatgcttt gatgatttta gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa
     5281 ttgatcgtaa tacaaaagaa tttttagcca ctgaagcagt gatagaaaat tatcaagaac
     5341 ggatacgtga tctgtacttg gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa
     5401 tacaagcact gattttgtcg atcgaacggg atggattgga tgaaagttat cgttcgggca
     5461 agctttcttc taatggttat cgtttttact cccgttttct ttctcgattc gaacattcta
     5521 tcacaagcca gattttgtct tttatcggct tttggttcat tgttgtccgt aggttaatgc
     5581 gaatcgttct ccatccgaaa atgttttggc aaagaagata tgctgatcgt cagacattta
     5641 ttagtgatga agatatccaa gaaatcagag agacatatca aaaaaatact cagttgatta
     5701 ttgaaagttt agataacttg actgatattt atgatgaaac gatggttcaa ttttttatcc
     5761 agcaacgcaa acttgaagga ataaaaatga actcggggaa tttgatttct tcgtggatga
     5821 tccagcaaga tagtctgttt acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa
     5881 tcattgatga atacgaagtt gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca
     5941 ggaatatcaa tttgttagaa tcttatacga tgaacaaacc atctgataac ttttctttcc
     6001 gatttgcgtt tcgagcaaag tcaaaaaaaa cgagtccaat aaaataaaaa ggtaaaaatt
     6061 ttcaagtttt ataaaaaaca aaacatatca gaacaggatc gaatcggtcc tgttcttttt
     6121 ttgagaacac aaaaagaaca aataccaaaa aaattttcct gaagttcact atttttatga
     6181 tacaatatcc aagataagga gggaacatgt tgaaagaatt aaaagtaact gacctcaaaa
     6241 aaacttatgg tgaaaaagat ttgtttgacc agatttcttt tttggtccac gaaaaagacc
     6301 gtatcgggtt gattgggacg aacgggacgg gtaaaacaag tctcttaaat attattgccg
     6361 gtatcgatag cagcgacggt gatcggcaaa cagtgtttta tccaactgat taccgtattg
     6421 gttatctttc tcaagcagct gaattttccg atgaattaac tgttctccaa gcagtctttc
     6481 aaggaaacag ccccttgatc caaactgttc gagcatatga ggaagcattg atcgaactag
     6541 gggaaaacgg agaagaccct gatgtgcaaa aacgttatgc taaagcagaa gaacaaatga
     6601 ataaagaaga tgcttggact acagatacca acgcaaagat tattttgcaa aaattaggaa
     6661 tcagtgaatt agataaaaaa atcagtactt tatcaggtgg tcagaaaaaa cgagttagtc
     6721 tggcgcaagt gttgattgat gaacccgatt tactattact agacgaacca acaaaccatt
     6781 tagattatga agcgatcgag tggttggaaa actatttgaa acaataccgt ggcgcactat
     6841 tgatggttac ccacgatcgt tattttctag atagagtagc aaaccgaatc ttcgaacttt
     6901 catttggaaa attatacgag tataaaggaa attatgaagc ttacgtctta gaaaaagcag
     6961 aacgtgatcg tgtagaagta gaacaagaag aaaagcgcaa gcggctttat aaacaggaat
     7021 tagcttggat gagggctggc gtacaggcac gtggcaccaa gcagcaagca cggatcaacc
     7081 gtttcgaaga tctaaaggag aacttgtacc aagtaaacca agaagatgat ttagagctga
     7141 acctagcgac acaacgttta gggaaaaaag tactggaaat caaagatggc agttaccgta
     7201 taaatgacca aacactgcta gaacatttag atttattgat tcaatctcgg gaaagactag
     7261 gtattactgg taaaaatggt gcgggaaaat cgacattgct taatattcta gctggcagaa
     7321 ttcccctgga tagcggtaca atgtctatcg gtgaaacggt tcatcttgcg tattatacac
     7381 aagaaaatga agaaatggca ccagataaac ggatgattgc ttacttacaa gaagctgctg
     7441 aagaagcaaa aacagcagat ggcagtcaaa tcggagtagc agaattactt gaacgatttt
     7501 tatttccaag atttatgcat gggacactga taggcaaact ttcaggcgga gaaaaacgcc
     7561 ggttattttt gttgaaactg ctgattcagc aacctaatgt tttattattg gatgaaccga
     7621 ccaatgattt ggacatagca acattgacga ttttggaaga ttattttaga agttttccag
     7681 gtgctgtgat cactgtttcc catgaccgtt atttcttgga caaggtagca gataagttat
     7741 tagtattcca aggaaacgga aaacaagaac tttattacgg aaacatgtcc agttatttac
     7801 tgaagcaaaa agagacccag cagcctgctg aaaaagcgaa accaaaaata cagagcaagg
     7861 aacctgctga aaagaagaaa ttatcttaca tggaacaaaa agaatgggaa acgattgaag
     7921 atgagattgc agaacttgaa gaaaaaatct cattgctcca agaagagatg aatcatcaag
     7981 gagataattt cactcgatta caagaattgc aaaacgacgt atctgaaaca gaagcacaac
     8041 ttgaagaaaa aatggcacgg tgggaatact tgagtgaatg ggtagaagat taggaggaaa
     8101 aaggatggaa caagcgtatc ttgacttagg aagaaaattg ttggaagaag ggcatcgtaa
     8161 agaagacaga acaggcacag gcacattaag cttattcgga taccaaatgc ggtttgatct
     8221 gcaaaaaggg tttcctttgt taacaacgaa acgagtacct tttggattga tcaaaagcga
     8281 attgctttgg tttttaaaag gagatacgaa tatccgctat ctattacaaa ataaaaacca
     8341 tatttgggat gaatgggcat ttgaacgcta catcaaaagt ccagactata atggaccaga
     8401 tatgactgat tttggccata gatgtttgac agatcctgaa ttcaatgaag aatataaaaa
     8461 gcagagcaag cttttttgcg agaagatcct aaccgatgat tcctttgctg aaacatacgg
     8521 cgatcttgga cacatctacg gctatcaatg gcgtcactgg gaaacaaaag acggtggctt
     8581 tatcgatcaa atcaaacaag tgatcgaagc aatcaaaaag actcctgatt ctagaagatt
     8641 gatcgtttct gcatggaatc cggaagatgt tcctagcatg gctttgcctc cttgtcatac
     8701 gatgtttcaa ttttatgttc aagagggacg gctaagctgc caattgtatc aacgcagtgg
     8761 ggatgtgttt ctaggcgttc cgttcaacat tgcaagttat gcgttgctga ctcacctaat
     8821 tgcacatgaa acaggtttag cggttggcga atttgttcac acattaggag atgctcatct
     8881 ttatatcaat catatcgaac aaatgaaaga acaattgagc cgagaaatac gcgcatttcc
     8941 gactttagtt ttgaatcagg aaaagcagtc agtatttgat tttgagatgg aagatatcca
     9001 attagaagga tatgacccac atccggcgat caaagctcct atcgctgttt agaatcagaa
     9061 aggatgagaa aatgttagct gcaatttggg cacaggatga aaacggattg attggaaaag
     9121 aagaccgtct tccttggcgt ttgccaaatg atctgaaatt cttcaaacaa atgacagaag
     9181 caaatacact agtgatggga cgaaaaacat tcgaagggat gggtagccgt ccgttgccga
     9241 atcgtcagac gattgttttg acgagagatt cttcctataa agcagaaggc gtgcatgtca
     9301 tgcatagtgt ggaagacgtg cttgctctgg aaaaagaaac agacggtatc tttttcatcg
     9361 ctggagggtc agctgtttat caagaatttc tgccattttg tacgatatta taccgtacag
     9421 tcatccatca tgtattcgat ggagatgctt attttccacc tgttgactgg tctgattggt
     9481 cactgatcaa tactagtcaa ggagaaatgg atgaaaaaaa tcgttatccg catcaatttg
     9541 aaacgtatca gcgtaatgag aatcctgtaa aaagcgggac aggcgaattg acgatataaa
     9601 aaagctatgg atagactagc tgttatttag agctgtccca tccatagctt tatttttttt
     9661 attaagtaaa gaataaaaca gagaagtaca taaaacctgc acctaacata acaaacaaat
     9721 gccaaacaac atgcatatag cggatagaat tcaagctata aaagaatgcg cctatcgtgt
     9781 aagaaagccc gcctgctacc atcaaaataa caccagtcat tccaagagag tgataaagag
     9841 gaacagcagc cacgacacag agccacccca taaaaatata gatgatcgta gatacttttg
     9901 atactctctc ccgtttatgc aacgtcaacg ctttatatat aatgccaaga atcgcaaaca
     9961 accagattaa tacaaataaa gtccaaccta gccatcctcg gatactcagc aaacaaaaag
    10021 gagtataact tcctgcaatc aaaagaaaaa tcgaactatg atcgaatact tggaacacct
    10081 tttttgcttt tgtaaaaatc aatgaatgaa acaaagtaga cgttaaaaat aaaagaatca
    10141 tcatcgatcc atagatagca taagagacaa tatggaggga ggaatgcaag tgggcacctt
    10201 ttactaaaag aaccactagt cccgcgatac tcaatccagt cccaattcca tgggtgactg
    10261 cattaaacac ttcatttaat atttcatacg ttcgattaaa tttcgttttt tccatgaaaa
    10321 cggctccttt actaggtgta attaatgaaa aatcattatt tgtctgttat actaaaaata
    10381 gaaattgtct tacatgtagt ttaacatgaa taaggcatct tgttaaagaa agagagagag
    10441 aaaataatga caaatgtaaa aatcgtaaca gattcttcat gtacgatgga aaaaaatgta
    10501 cgcgatgctt taggcattcg tgtcatgccg ctatccatca tgatcgacgg aacagtatat
    10561 cccgatgatg accaactaga cggcgaaaga tttatggaaa tgatggcgaa agcaaaaaat
    10621 ctgcccaaaa caagccaacc accaattgga gaattcatct cgatgtatga tgagctagga
    10681 gaagacggca gtgaagtgat ttcgatccat atgacaaaag gcttgagtgg aacagtagaa
    10741 gcagcaagac aagccagtaa tctaacaaaa acgaaagtaa cggttatcga cagtgatacc
    10801 actgaccaag gattatcttt tcaggtaatt cgagcagctg aattagcaaa agaaggtaaa
    10861 acagtagaga aaatccttga agaagtcaaa aaagtacaag acaatacaaa attatacatc
    10921 ggaatatcca cactagataa cttggtaaaa ggtggaagaa tcagccgaac aactggtttg
    10981 ctgtctagtg ttttgaacat acgcgtggtg atgaatttcg cccattctga attaatccct
    11041 gtaactaagg ggcgaggaaa gaaaacgttc gataaatggt ttaataattt gaaaaatgaa
    11101 ctgaaagaat tgccaaatgt gcgacaaatc ggtatttcac atgcggatgc aaaagaattg
    11161 gcgcaagaat tcaaagaggg tttgcaagaa ttattcccgg acatgcatat ccctgtactg
    11221 catacgaatc cggttatcgc tacgcatact ggtaaagacg cgtttgcaat catgtattat
    11281 gtagattagt cgaaaagagg ataagccctc agcctgaaag gcttgcagct tattctcttt
    11341 ttctttataa aattaggaaa ataattgttt tcttttttca taaagactac ctttttgata
    11401 aaatttttga taacattaga accaatcgaa tacaagaggt gaaaaaatga aattaacgaa
    11461 acgacatttg atcctcgttt tgttcccatt attaattgcg atcatcatct atgcaggttt
    11521 agctgcttct ataccgaaag ctggcccact aatgaaacca gtaactactg tgagagaaga
    11581 tccaaataaa aaagaaaaac ttcactacat agctatcgga gattcgctga ctgaaggagt
    11641 aggagatcaa accaagcaag gaggattcgt tccccttgtc gcaaatgatc taaaagaccg
    11701 ttataatttg accgcggttg aaacagaaaa ctatggtgta aatggtgaac gaagtgacca
    11761 gatcttaaag cgagtaaaaa caaaagaaga aattcgaaac aatatcaaaa cagctgattt
    11821 tatcacactg actgttggcg gtaatgattt gatgaaagtt atccaaaata atttattcgg
    11881 gttaacggtc aattccttta aaaagccact gaaaaactat caggaaaatg tcacagaact
    11941 attggaagaa tttcgatccc tcaataaaac cgcacctatt tacattttag gcatctacaa
    12001 tccattttat ttgaactttc ctgagatcac tgacatgcag acaattgtta acaattggaa
    12061 caaaggaaca gaaacggtga caaaaaaaat gccgaactgc tactttatcc caatcaatga
    12121 tttgctttat caaggattga atcaggaagt aggtgtcaca gaagaaggaa cagatcaaac
    12181 aactagcacg acagaaaatc taaatggcat caaaaataat gtgctctatg aagaagatca
    12241 tttccatcca aataatttag gttatcaatt gatggcaaat gcagtgagag atcagctgat
    12301 cgagacacaa aaccaatggt tagagaagga ggatgatcaa taaacatgaa aacaagatct
    12361 gacgtatctt caaacaaaac aaagaaaaca aattactgga aattcgcctt ccttatcctt
    12421 cttggggtag tacttggttc gaccgtattt ttaggcagcc gtatttttgc taatcgggaa
    12481 ccagaactcc cagaaatacc agctcttacg gaaagacaag gagatcctgt cttaacgatc
    12541 aattcaaata aagaaaaagt caatcaaatc atcagctttt tccttagtga gtatcaaaaa
    12601 gatagtgata ttgaatataa attttatttg gaaaatgaag cactactcaa tggtactttc
    12661 gaagtactag gttttcctat cgatttctat ttatattttg atccgtatgt gatggaaaac
    12721 ggaaatgtcc aactaaaagc tagaagtctt tctatcggaa cattgagttt gccaatcaaa
    12781 gatgtgatga atatgatcaa gagaaactat aaattaccag attggatcga agtcaataca
    12841 gatgatctga ctatcatgct taggttggat caattccgaa tgcaaaacgg gatgtatatt
    12901 aaagcagata aaatcgactt agtaaatgat gacattcggt ttagtctata tttgccaaaa
    12961 gatacctctg atgcttcaaa caactcaaat accaacaaaa ctagcaccag cagtactgaa
    13021 agcaagacaa aagaaaataa aaaatcatcg gatgagtagt attagagggg gaatcataat
    13081 ggaacgagca atatttgcag gaggctgctt ctggtgtatg atccagcctt tcgacacgct
    13141 tcctggtatc catacagtga tgtcaggata tactggaggg catgtaccag atccgactta
    13201 tgaacaagta aaaagccaaa cgactggaca tacagaagca gtggagatta tctatgatcc
    13261 aacattgatt tcatatgaac aattagtaga actttactgg cagcaaactg atccaactga
    13321 cgctttcgga caattcgagg atcgcggtga caattatcgc ccggtgatct tttatttgac
    13381 agaagaacag cggatcattg ctgaaaaaag taagaaacag ttagctgaaa gtggacgttt
    13441 cgaacagcca atcgttacaa aaatcgaacc agcccaacca ttttatctag cagaagaaga
    13501 ccatcaagag tattataaaa agcatcctga aaattttaaa agaaaccatg cacgccgggc
    13561 tgcctttatc gaagaaaatt ggaggaacga agggtgaatc gaagcttttt tcattattta
    13621 atgacactgc gaggcggtgg cacacatgat gctttatctg ttttcgctac agaagccggt
    13681 aaagacatcc aatttcctaa gcacagctct agctacgaag aaatttctaa ttatctggaa
    13741 ctgaatgtcg actatttgcc gtccatggat atctttgacg aagcatggga aaaatattta
    13801 gaaaataatc atgacatata agagatagtt ttgtacttta tgatcaaaaa agttttttgt
    13861 ccaatagcct aattgaaaga attattttta tttcagctat ctagtcgcat ttttttgata
    13921 tttttc
//
LOCUS       DAZULG010000073        13671 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000073 DAZULG010000000
VERSION     DAZULG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.075"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..1501
                     /locus_tag="ACG9CY_001941"
     CDS             74..1501
                     /locus_tag="ACG9CY_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938443.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1593..1958)
                     /locus_tag="ACG9CY_001942"
     CDS             complement(1593..1958)
                     /locus_tag="ACG9CY_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4938444.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1955..2320)
                     /locus_tag="ACG9CY_001943"
     CDS             complement(1955..2320)
                     /locus_tag="ACG9CY_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4938445.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2512..2760)
                     /locus_tag="ACG9CY_001944"
     CDS             complement(2512..2760)
                     /locus_tag="ACG9CY_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938446.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2759..2887
                     /locus_tag="ACG9CY_001945"
     CDS             2759..2887
                     /locus_tag="ACG9CY_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS4938447.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2944..4638
                     /locus_tag="ACG9CY_001946"
     CDS             2944..4638
                     /locus_tag="ACG9CY_001946"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS4938448.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4738..5034
                     /locus_tag="ACG9CY_001947"
     CDS             4738..5034
                     /locus_tag="ACG9CY_001947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938449.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5412..6545
                     /locus_tag="ACG9CY_001948"
     CDS             5412..6545
                     /locus_tag="ACG9CY_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS4938450.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6576..6896
                     /locus_tag="ACG9CY_001949"
     CDS             6576..6896
                     /locus_tag="ACG9CY_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS4938451.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6925..7389)
                     /locus_tag="ACG9CY_001950"
     CDS             complement(6925..7389)
                     /locus_tag="ACG9CY_001950"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4938452.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7520..8773)
                     /locus_tag="ACG9CY_001951"
     CDS             complement(7520..8773)
                     /locus_tag="ACG9CY_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4938453.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8802..9119)
                     /locus_tag="ACG9CY_001952"
     CDS             complement(8802..9119)
                     /locus_tag="ACG9CY_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4938454.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9145..9465)
                     /locus_tag="ACG9CY_001953"
     CDS             complement(9145..9465)
                     /locus_tag="ACG9CY_001953"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4938455.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9929..11344)
                     /locus_tag="ACG9CY_001954"
     CDS             complement(9929..11344)
                     /locus_tag="ACG9CY_001954"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4938456.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11361..12677)
                     /locus_tag="ACG9CY_001955"
     CDS             complement(11361..12677)
                     /locus_tag="ACG9CY_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4938457.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12827..13525)
                     /locus_tag="ACG9CY_001956"
     CDS             complement(12827..13525)
                     /locus_tag="ACG9CY_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS4938458.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4340 a         2779 c         2355 g         4197 t
ORIGIN      
        1 gatgatttca aaaagaaaac atgtcataat atttcccata ttgtgcaaaa aaatacaagg
       61 cggaggctct actatgattg aagaatatat tgagaaaaat attttaagac agctgttttt
      121 atgtgggcaa ttctatgtaa ataaagaagt caatctagaa aagctctcca atctcctgca
      181 tgtttgcaaa accactttac tcaatgatat caataacata aaaaaagaat ttgaagaaca
      241 gatcgcttat acacatcggg aaaaggaccg ttacacttta tatttttcag aacatatacc
      301 tcgatgcaag atcatgcagc aattgtctca aaattcgttg tttttaaaaa cttgtctgct
      361 ttatttggaa gaagatgaac ctgattatct tcaattaact gaatgtgaat tcatctctgt
      421 cagcaaagct tattcattaa aaaaacaagt attagcttat tttaatgact gcgggatcga
      481 aatcgaccgc tactcccctc gattcactga aatggaaaga cgtctgctgc ttcttaatgt
      541 gtcgtatcgt ttaggcggtt ttaattcttg ggagctacct gaatcgtttt ttgaacgtgc
      601 cgatcgtttc attgaatccg taacggaaaa tagtggcaga ttttatgata aagaaaataa
      661 agaaatctta tcgattggtt ttgctatcag tttcctacgc cagcaagtgt gtgctgtgac
      721 aatagattca aaatttatcg aagaaatcaa aaaacgtcct atttacaatt atgtcgaatc
      781 agcatgggaa aacactgatt tccaaaccta ttataaaaaa gaagaattcg cctttatttt
      841 gacactattc aatctttgta attatggttt ccattcttat cagttgattg cggaagactt
      901 tcaacagcta caccaagtgt ttattgacaa tactcccgaa ataaaagaac tagttgctac
      961 ctttgaatct cacttcaatc aagaattatt cggaaaccaa ccgttcgaac gtgcgctgat
     1021 tcatctgatg cgttcggctt gggataacta tcaattattc atgccagaaa aattttatct
     1081 tttaaatgag gaacagacaa atttatataa agaagtccag acaatttttt ccagctggtc
     1141 cagccagtta ccttatgatc tacggttgaa tcctaattgc atgcgggctt ttgtgatcga
     1201 attgagcgga attctccgtc tgacaaaaga acatctgact atctatatcg tcacaaattc
     1261 ggatgttcat tatctgattt atcgggaagc cttggaagct gtaactactt tcgattttca
     1321 agttgcacca acaatttatt caagtatatc tgatataaag aagtatgcgc agcagtcctc
     1381 aaaccgggta ttatgtgaac gtacattata tacaccagat gcggtccaat atgaaaatat
     1441 tattcctatt tcgatcaata cgatcgatcg agctatcatt tcagctgtgc aaaacaaata
     1501 aggggctgtg acaaaaatca tgtcacagcc tcttatttgt tgaagatgcc catttctgta
     1561 acaatctctt gatacacgtc aggctcacaa cctcacttat tttggacatc tacgggtccg
     1621 atctctttat ttaaaaaata agaaatgatg gttcggataa ctaccaacgc cgctagttgg
     1681 aaaatatcct gcaaggtagg tttgataatc gattcgatga tcccagctga aacaagaatt
     1741 tccaaactta gaagtatata gcctcccaac atctttttaa tagcattgtt ctcgctgact
     1801 cggtcgcttt gcacttttgt atggacaaat aaatatttca aaaaatcctt gattgataga
     1861 caaacacccc aaaccaataa acagatagca aagatatcaa gtcccagtac aagtttttcg
     1921 aaatatggaa gaagaaactg catcaactct gtcgtcatcc tacctctcct cttctttgtt
     1981 tttctttgtc ttctatttcc tgattcaaga aataagaaat gactgttcga atgataataa
     2041 taagtgcaag cttcaaaata tctgtccaag tcggcttgat aactgactcg atgatatcag
     2101 caacgactag tacttccaag cttaacaaaa tataacttgc tagcagtttt cttgtatctg
     2161 cattacgagt attacgttct gcatagtttt tcttgacatg agaccaatca aaaagtttga
     2221 atagaacaag aacgatcccg acaattaaga taccgataga acaaatattt aaaatagcaa
     2281 tgatccattc tagaaattca attaaatttg agcgaaacat atacgctacc tactttcttt
     2341 taatttgttc ctattctttt attgtaaaag aaagttacct tgttcgtcta tttaaaagtt
     2401 gttctaatga taacggttcg cttaaatttt tctttttcac tctcccattt ttctccctcc
     2461 atccataaat cgtggaagta tccaaaacaa cgtagcaagt taaaataagg cttagtccgt
     2521 catcagcttt tcaaagaagc actgtctcgg tgcttttttt gtacccaaat ttctaaaaat
     2581 agaaccatac acctcctcaa atcttttctt cttacctacc ctatttttat ttacaggaaa
     2641 atcagacact tcttcttttt tgtcaaaatt tcctttttct tctcgtccta tcatttggca
     2701 ttctaataaa aacgaactat ccttggatat agaaagagaa aacaaggagg cgtttttcat
     2761 gagtaaaaaa ttaatcaaag tcggcatcgg tttaggtttg cttgcccttg gcgctgcata
     2821 tctaggaaaa aagacaggac tattcgaaga tgacagtcat ttatatgatg aattcgaatc
     2881 aatctagcat cctatttagt tgaatcacta cggacatact tggaagacat tggaggaatc
     2941 acaatgagaa aaagtaaagc aataatgatt ggtgccggta ttgccaatat ggcagcggct
     3001 gtttatttga ttcaagaagg caaatggaaa ggggatcaga tcacctttta ttcattggat
     3061 gatcacggtt caaatgatgg tgcaccgacc gacactgtag tggatgaata ttggaataaa
     3121 aaccatccat tagaaaacca aaaaggctat attgctcgag gcggtcgcat gcttaattac
     3181 cgtacctatg tagatttgat ggatctgtta agtcgaatac cttctgttac tgagccaggg
     3241 atgactgctg aagaagatac gagagatttt gatgcaaaac accgaacctt tgacaaagca
     3301 cgtttgatgg aaggtggcaa aggaatcatt gatggcggaa aactgggatt caataataaa
     3361 gaccgtattc ttctgactaa actgattgct atgcctgatt cagaagaaga aaaactagac
     3421 aatatcacta tcgaagaata tttcaaagat gatccacact tttttgaaac aaacttctgg
     3481 tttatgtggg aaacaacatt tgctttcaga acacgtagtt ctgctcaaga attgcgtcgc
     3541 tatatgcatc aaatgattta tgaattcact caaatcgaac atctagtggg cgtcaatcga
     3601 acacgttaca atcaatacga aagtattatg cttcctttga tcgagtactt gaaagaacaa
     3661 ggatgcaaaa tcattttgaa ccgccgtgtc actgactggg aattcaaaga aacaccgatg
     3721 caagatgaaa tcactgtgac tggtttgaaa attaccaata cactgactca agaagaagaa
     3781 tacgttgtgg tcgatgaaga tacagcagtt attttcacta atgggtccat tacagattca
     3841 gctactttag gagattacga aacaccagct gtagaaaata tggattatgg tgcagcttct
     3901 agtctctgga aaaaagccag tgaacgtttc tacaacttgg gaaatcctga caaattcttt
     3961 gctgatcgag atgcaagcga atgggtcagc tttaccctga cgacgaaaaa ccatttgttg
     4021 ttgaatgaaa tcacccgtat cacgactcaa gaaccaggaa atgccttgaa ttcatttatt
     4081 tcaaccacac cgatcacgcc gttaggccaa aaagatgtca atatgtctat cgtcgtgcat
     4141 catcaaccgc attttacgtc tcagaaacca aatgaaacag ttatttgggg ttatttcctt
     4201 tatccaagac gacgtggcga atttgtggat aaacagtata ttaaaatgaa tggaaaagaa
     4261 atgctagaag aactgattgg acaactttca aaagtagatc ctggaccagt gaatattcgt
     4321 gaaaaagaaa cagaaatttt cgacagcgta ataaataata tccctgtcta tatgccttat
     4381 gcttccgcgt tgttcaataa tcgtgccaaa agcgatcgac caaaagtcat tcctaaacat
     4441 tcgaccaatc tggcctttac aggagaattt gtcgagcagc cttatcagat gatctttact
     4501 gaacaaagtg ctgtacgttc tggagaaatc gctgctttcc attttgcggg tattcctatg
     4561 tcccgtcttg tcaaaacacc gcgttacgac aaagatattc ctactctagc tcgtgccgca
     4621 aaaaaaatgt ttgaataatc aagaggttgt gaaaaagctg ccctgcatca agtaataaga
     4681 acaaccaggc taaaacagct tcttatgttt ttgatttggt tggacttatt accgcagatg
     4741 caagcttttg aacaccgttt accaagaggt tgtgaaaaag ctgccctgca tcaagtaata
     4801 agaacaccca agctaaaact gcttcttatg tttttgactt ggttggactt attaccgcag
     4861 atgcaagctt ttgaacacca tttaccaaga ggttatgaaa aagctgccct gcatcaagta
     4921 ataagagcag ccaagctaaa acagcttctt atgtttttga cttggttaga cttattaccg
     4981 cagatgcaag cttttgaaca ccgtttacca agaggttatg aaaaaggcgt ttagtttcga
     5041 gtatgaagaa aaattttgtg aaaatggttc ctcacgtttt tgcataaatt tgacttaata
     5101 ccgagaaact gatcccttct gtcataacct ctttttattt atgtctgttt ctttttgaaa
     5161 acaactattt tttcaacgtt tactctcttg ccaatccaac atcatctaac ggatacaact
     5221 atttcaatga ttaccattac actaaaaaat cggaaacaca cctctccgaa cacaaataca
     5281 taagactata gaatgtttca tttaagaaaa aaagcgtttc attatcaaaa aggtaagatc
     5341 tcctttaaaa tgaaattatc tgtagtacta ataatgcaga caattttatg aatctgaaag
     5401 gagcatctcc aatgaaagca gtcacttggc aaggaaaaca gaaaatggaa atcagaaacg
     5461 tagatgatcc aaaaatatta gaaccaacag atgcaatcat ccgtatcaca gcaacagcca
     5521 tttgcggctc cgatctgcat ctttaccatc atggtgaagc agttttggaa aaagggtatg
     5581 ttgtaggaca cgaaccaatg ggaatcgttg aagaagtggg atctcaagtt caaacattga
     5641 aaaaagggca acgtgtggtt atccctttta atattagttg tggtcattgt catttctgcc
     5701 tgaatcacat ggaaagccaa tgtgatgaat caaatccaga tcagttatat ggcgggcttt
     5761 tcggatttgg caagttgaac ggtaatcatt ggggtggtca agcggaatat ctaagagttc
     5821 cttatgcaga ctctacttca tttgttgtac cagataatga tcttcctgat gaaaaagttt
     5881 tatttctatc agatattttg cccacagctt attggagcgt tgaaaacgca ggagtaaaaa
     5941 aaggcgatac agttgtgatt ctaggttctg gtccagtcgg tctattcgct caaaaattcg
     6001 ctgttatggc tggagcagag cgcgtaatcg cagtagatcc agtacagcac cgtctagata
     6061 aagcggctaa ttacaatggg gttgaaactt atttcttaga agacacggca caagctggaa
     6121 cagaattgta tgaactggct aaaggtggcg cagatgtcat tatcgattgt gtaggaatgg
     6181 acggattaga gcctgtcaaa gaaaaagcga aaaatttagt aagcttacaa agcggcacaa
     6241 tctctccatt acagatggca tcgcaagctg tcaaaaaatt tggaacagtc caagtaactg
     6301 gtgtttatat gactccagct tcttcttacc cgctgcaaga attttttatg cgggatatcg
     6361 ctgtcaagca tggacaggca ccaattatcc atctaatgcc aaaaatttac gcaatgattg
     6421 cagaaggact attcgatcct acacaaatca ttactcattc gctgccacta tcagaagcag
     6481 cacaagcata tgagatcttt gacaaaaaag aagataaaaa tataaaagtt gttttgaaac
     6541 cttgaattca gtaaaaaata caggtgattt tctttatgcc caaaatcaaa cgaaataccc
     6601 atgagatcga aataggaaaa gaagaagata tcgaaatacg aggacgtcgc ttccgcttct
     6661 atttccaaaa tcgttacaca ttgatctcac tggctgtcga tttattaacc gggattttct
     6721 atatttttgg aagccttgct tccttaacta aaatccccga tcgatatggc acctattttt
     6781 atttggctgg tgctattttc ttgacgattc gtcctattct cagaatcgta aggaacattt
     6841 ttatttacga cgaaaaaaaa gcaatgaaga gaacaagcaa aaaaaaagag gcatgagaat
     6901 ttttcaaatc tcatgcctct tttttcaccc tatcgtatca ctttgataca gacgagcggt
     6961 cgtcccactt tcttccttga ccattgtcaa aaatgtccac tcgtatcgtt cataaaacgt
     7021 ggtatgatct gtaattaagt aaagtttttc gtacccaagt tgatgcattt cttttctgat
     7081 ttccgctaat atccgcttgg ctatcccctg cttacgcttt tccggttcaa caaacagggc
     7141 acaaagattt ggtgtcagat cagggcggtt atggaagtcg ttttcaatga cgcccgctcc
     7201 tccgacgatg gtttcttttt catctaagac cacataccat tgaggaatac tttccttatt
     7261 agcgattcca gtacgaatac tttcctcata cacaacagca ggaactcccc attttgatga
     7321 aaaccaattc gcgctttgtt ttactaattc tgggtgttct cttaatttga tccagttata
     7381 tgtaaccaat gtttaaacct ccgactaaaa taaaacgttt acaaaaatta tacaagtctt
     7441 tatgaaaatt agcaatgaca acaatagtga tgtaaaaaaa aaacggaaaa caatcacttg
     7501 ttctccgttt tttgattaat caagcatcta aagcttcttt tcttctttct tggatcgtag
     7561 ctaccttgac gaacggtaga tagatcaaaa tagaaagaac caaattgaca gcggctagta
     7621 caccacctgc aatacttttt gttgccaata caccgccaat aattggtgga gttacccatg
     7681 gaggcataac ggtagctgct ggcactaaac ctgtagaagt cgcaaagtaa gcaacactaa
     7741 ctaagaccat tggtgtcaaa ataaatggaa tgaacatgat cgggttcaac acgataggta
     7801 acccaaacat cgttggttca ttaatattga agacacctgg cgcaatcgac aagtttgtta
     7861 caaccatata tggtttgttt ttacgtccta ctaaataaat agctaacagt aaacctaaag
     7921 ttgcacctgt tcctcctaag ttgacaaagc tgtcaaagaa aggtttgttc acgataaacg
     7981 gtgcgctatg cccagctgcg attgcattaa cgtttgcttc aatggctggc gcattgattg
     8041 tttgcatcaa aggatcgacc atattcgctc cgtgtaaacc aaagaaccat aagaatggtg
     8101 tgataaatgc aagcagtaag gcagatggat aagagtttgc aagtcccata aatggttctt
     8161 ggaccactgt atagaaagaa cctacgatat catcaacacc gaatgcagca aaaattgcag
     8221 caactaaagc aaacgcagag atagtgatca ttgctggtag taatgaagca aatgcttttg
     8281 caactgccgg tggcacacca tcaggcattt tgatgatcaa tttttcattt ccgcttaaac
     8341 gtacaaagat ttctgttgaa ataatcccaa caataatagc aataaacaaa ccgtttgagc
     8401 tcatacctaa tgctccgcct aaagcgaaga aagagcctaa agaaatcaca cctgcggaaa
     8461 caccgtcttt tcggtagccg ttagctaaat gataagcaag caagaaagca atcaagacag
     8521 aaagaatggc aaatgtaccg ttccaagcag cagccccgaa tcctttccat gattcaccac
     8581 caaaaattga gttcatcaat tcttggaacc cgggaatcgg taagttattg atcattactg
     8641 ctaatgctcc taagatcatt aacggcatac tcaccataaa tgagtcccga atcgcaacta
     8701 aatgccgttg tgcaccaatt tttgatgcta caggtataaa atgtttctcc ataaatgcaa
     8761 caaatttatc cataaatctt ccctcttttc tttactgtag attacgcgtt caatcgacga
     8821 tacagatcaa tgatctcttt tgctaaatct gtaaaagcta tagatgtcat cagatgatct
     8881 tgactgtgga ccgtcaaaag cgtgacttca acgtgatttc cttgtgcttc ttgtgtcagc
     8941 atctctgttt gagagtgatg tgcttggact aaagattcct ctgcatcttt gattttttca
     9001 tctgctaaag tgaaatcacc ttttttcgca gcagcaatcg cttccattgc atcgcttttt
     9061 gcatttccgc caaacatgat cagacccatg atggcttcta gatttttttg atcttccatt
     9121 tattgtatcc tccgataatt tttattattt gtttattagt ttttctgctt ggtcgagtac
     9181 tttttcacca ttcatcatgc catagtcagc catattgatt acatctaaag gaatgccttt
     9241 aggtgctagc ttttcagcaa attgattttg cataaagcgt acttgcggtc ctaaaagtaa
     9301 aacgtctaca tttttagatt ctagttgatt atccgcatct gaagcagata cagcaaagat
     9361 gtctgcttcc attccttttg cttctgctgc cttttgcatt tttgtgacta ggagacttgt
     9421 actcattcca gctgaacata caagcatgat tgttttcttt tccattttaa acacgttcct
     9481 ttctatactt acacttgtat ataagcaagt agcgtgccaa ctttaaaagc gctttaaaaa
     9541 gttgatatca tcacatttaa ggtctatacc aagttgcata cacactaaaa aagaaagcgc
     9601 acactaaaca cacaaattaa tttttattac cttttttctt ttaacgtgca caaaaaaagt
     9661 tgtgacacaa aacttgtcac aacttttttc aaataaacgg tattcaaaag cttgcatctg
     9721 cggtaataag tccaaccaag ttaaaaacat aagatgttgt ttttactttg ctattcttat
     9781 tacttgatgc aggacagctt tttcacaacc tctcaataaa cggtgttcaa tcagctgacc
     9841 ctgggtatta gctcaaaacg ctggattcac aacctctttt atttcaaata accagtaaat
     9901 acaatgaaca tctacaaaaa acttctattt atttatagat ttcgtcaggc aaatccaatt
     9961 ctctcttatc aatcaaagtc tgataccacg aagcagattt tttcaatgtt ctgcttcgtt
    10021 catcgttcaa ttcgattcga actaatccat atcgattctt aaatgcattc attggtgaca
    10081 cacagtcagt gaatgcccat aacatatagc cttcgcaatt tgcgccttct tctgttgctt
    10141 tgatcaagta atgcaaatgc tgtccgataa attcgatacg gtaatcatct tgcacgattc
    10201 ctttctcatc catatactgt tcttcatatt ctcttcccat accgttttct gttatcagcc
    10261 aagggatatt atgataattt tcttttagat acatggctat atcatacatg attttcggat
    10321 agatttccca tcccctggaa ttattcattt tcttcccagg taaatcaaag acttcataat
    10381 atcgttcagg atggaaaggt gtctcttcat tccattgata acgaggggcg cgaacgcgtt
    10441 taggataata ttgattgatt ccaaggaaat ctactgtatg atgctgaatc gtttctaaat
    10501 cctctgttgc aggatcgaaa taaatcgatt gtttttgaca caactccagg aactcttctg
    10561 tgtagtatcc ttttaccatc ggatcaaaaa atactcgatt ataaaacaaa tcatacatat
    10621 aagcagcttt ttggtcttct ggagaattgg atcgagcata gaccatttca ggatttaaaa
    10681 tacagccaat ccttccacca taatttccgc tgcggtaaat ctctactgct ttagccgttg
    10741 ctaaaacttt atgataattc cacatcatcc acttttttgt gttttgttca tgcggccagc
    10801 gaacagcatc caaatagcaa cgcgtttggg gaacgattgg ttcgttgaat gtaaaccagt
    10861 tttttaccct atcaccgtaa cgatcaaagg caatttgagc atacttagca taaaaatcaa
    10921 ctacttttct tgaagaccag ccatcatatt tttccaaaag aaaagctggg acttcataat
    10981 gttccaaaca aatcatcggt tctacgtctg cttcgatcaa agagtcgatc atttcactta
    11041 tatgctgagc atattcttca tctactatac agttctcata atcagtaaaa aaacgagccc
    11101 aattgataga agtccgataa tgtgtcagat ggatggattt catcagatcc acatcctgct
    11161 ggtattgttc cataaaatta gttgctacgg ctgggccata cccattatgc caaacaaaat
    11221 gttcattttt ataccagcta tctataaacg aatcctgtcc ttcttttttt cctttccaac
    11281 cttccgtttg ccaagcagaa gatgcagcac caagaataaa attttttgga atagtaattt
    11341 tcatgatttc ctcctgtttt ttagatccca tcattttcca cacgatttgc tacttttaca
    11401 aatggcagat agatcaaaat agaaacaata atacaaacaa gttgcgtaat aactgcaccg
    11461 atatttcctg ctgttgccag ataagcattg acgatcggcg gcattggcca agggaccatt
    11521 acaactgctt tcgccgcaaa tcctaaagcg gttgcaaaat aaccaatcgt tccagtgata
    11581 agaggcgtta agataaacgg aatagctaaa attggattca gcatgatcgg cataccaaaa
    11641 ataactggtt cattgatgtt gaagatccca ggagcaaggg ataacttggt gatttgtttc
    11701 atatcttccc gttttccaac taacaatact gcaatcagta aaccgatcgt gactccagac
    11761 ccgcccatac tcatatacat atcccaaaat ggcatagtaa taatgttcgg tacttctttt
    11821 ccagcttcaa aagcttccgt atttacagca attgccgcta aaagcaacgg ttcccgtatc
    11881 ggcttcacca tttgatttcc atggatacca atcacccaaa agacttgcgc aataaacatc
    11941 aatagcaaaa tacccggtaa cccttgtacg acaccttcta atgggcgttg tacgacattg
    12001 taaatgatat cataaaggta cattcctgta agttctttga tgataaatcc cgcagccgcg
    12061 atacccgata cagtcagaat cgtcgggaat aaagcagaaa aactagcaga aacattcgtt
    12121 ggcacggtat caggcattct cagctgtagt ttcttctgtt tcctcaacca gcaataaagt
    12181 tcaatagaaa ggatggagac caacattccc aaaaataacc ctttcgtatc tgtatattgt
    12241 ttagccaata cgttttctac tataacagtc tgattttcat tcactaattc ataaacagtt
    12301 ggattaacag ataaataaga catcactgcg atgataccag ggaatacacc atccacttta
    12361 ttcagcttgg ctaattcgat ccctatcaga aaaacagcat agatggtcaa gaaacttagt
    12421 gtgacatagc tgatggcaga tgcgataggc tttaattctt ctaaaaaacg caagaaactt
    12481 atctgtgcta agccattttc cgagtcaaag accattgctg aaaacaatgt gccaaaagca
    12541 cccgtaataa ttaccggaat caatgcagca aatgcgtttt ttatcaccat gatatacctt
    12601 aaattattga ttctcgttgc cacgttccct aaaacattgg ctaatttatc cataaaactc
    12661 attccagatc ccctcattcc tgcttcttct ccttgacgtc atctcttttt gtctattagt
    12721 cagcgcttac atttcgatga acagaaacta tttactcctc ttttttacca aatataaaac
    12781 aaaaattcat ctgatgtatt tatcataaaa tacatcagat gaactgtcaa ttagtttctt
    12841 tttctcctat tattttctgt aattctctcc gatttattgc catatggaca gtcattgcat
    12901 caaacgcagc aagtgcattc cgcctttcga tggcctgaac gatctctgta tgaatatcga
    12961 tcgtctcgat tttcagatgc tggatatcat aatttttatt aaacaaagca attgattcat
    13021 taatgattgg gataatatgc agcaaggcta cgtttccgct agcttctgca atcatcgtat
    13081 gaaaatcgat gtctaattga acatgttctt gtgtgccttc tcgaaaagaa gtttcaatct
    13141 tttgcgttaa ttttttcatt tggacgattt gattcttcgt tgcatattcc gctgccaaac
    13201 ctgccatctt aggctccaat aaatatctca gttcaaataa gtctcgtatc atccgatcct
    13261 gatcttcaat caaagaaaaa ccgagtgggt cattggcaat ccctcgctta tcactgacaa
    13321 atgtccccgc tccttgccga acttcaagga tattcctgga agctagagcc cgcacagctt
    13381 ctcgcaccgt gcttcgcccc acctctaaat aatcggacaa ttcatattca ttcggcagtt
    13441 tatctcctac ttgcaactgc atttccttta aatatctaag gatcttgtca gtagttactt
    13501 ctactagggt tttattttct tccaaaccac atacctcctt ttcatctgat gtattttttc
    13561 gtaattatta taacacgttt gaatcatctg atgatttttt gtatacaaaa ttgattaatc
    13621 cgttcataga catacaaacg aagaagatat ttaccataaa aaagactagg a
//
LOCUS       DAZULG010000074        13622 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000074 DAZULG010000000
VERSION     DAZULG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.076"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..240
                     /gene="yaaA"
                     /locus_tag="ACG9CY_001957"
     CDS             <1..240
                     /gene="yaaA"
                     /locus_tag="ACG9CY_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS4938459.1"
                     /translation="KQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGEP
                     ENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            227..1351
                     /gene="recF"
                     /locus_tag="ACG9CY_001958"
     CDS             227..1351
                     /gene="recF"
                     /locus_tag="ACG9CY_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS4938460.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1348..3294
                     /gene="gyrB"
                     /locus_tag="ACG9CY_001959"
     CDS             1348..3294
                     /gene="gyrB"
                     /locus_tag="ACG9CY_001959"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS4938461.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3314..5785
                     /gene="gyrA"
                     /locus_tag="ACG9CY_001960"
     CDS             3314..5785
                     /gene="gyrA"
                     /locus_tag="ACG9CY_001960"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS4938462.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6021..6320
                     /gene="rpsF"
                     /locus_tag="ACG9CY_001961"
     CDS             6021..6320
                     /gene="rpsF"
                     /locus_tag="ACG9CY_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS4938463.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6370..6882
                     /gene="ssb"
                     /locus_tag="ACG9CY_001962"
     CDS             6370..6882
                     /gene="ssb"
                     /locus_tag="ACG9CY_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4938464.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6908..7144
                     /gene="rpsR"
                     /locus_tag="ACG9CY_001963"
     CDS             6908..7144
                     /gene="rpsR"
                     /locus_tag="ACG9CY_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS4938465.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7287..9260
                     /locus_tag="ACG9CY_001964"
     CDS             7287..9260
                     /locus_tag="ACG9CY_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS4938466.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9266..9718
                     /gene="rplI"
                     /locus_tag="ACG9CY_001965"
     CDS             9266..9718
                     /gene="rplI"
                     /locus_tag="ACG9CY_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS4938467.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            9992..11359
                     /gene="dnaB"
                     /locus_tag="ACG9CY_001966"
     CDS             9992..11359
                     /gene="dnaB"
                     /locus_tag="ACG9CY_001966"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS4938468.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12139..13431
                     /locus_tag="ACG9CY_001967"
     CDS             12139..13431
                     /locus_tag="ACG9CY_001967"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS4938469.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4531 a         2470 c         3000 g         3621 t
ORIGIN      
        1 aaacaacaag tcattctaaa tacggaattc atgacgttgg gccaaatgtt gaaagaagtc
       61 acagttatcg gtagtggcgg acaagcaaaa tggtatttgg cagaaaacac tgtgttagtc
      121 gatggcgaac cagaaaatcg tcgtggacgc aaactttatc ccggcatgat ggttgaagtt
      181 ccagaagtag gaactttttt tatggtaaaa aaaggaaacg acgataatga ggctgaataa
      241 gctgtattta aaaaattatc gaaattacga agagctgaac atcgaatttt ccaagaacct
      301 cgtcatcttt ttaggagaaa atgcgcaagg aaaaacgaat cttttggaaa gtatctacgt
      361 attagctatg acgcgaagtc atcggacaag tagtgagcag gaattgattg gctggactga
      421 cgatcaggcg atgattcaag gagagatcac aaaaggttca tccactcttc cattagaaat
      481 cttattatct aaaaaaggcc gaaaaaccaa gatcaaccat atcgaacaaa aaaagttaag
      541 tacttacgtg ggacaaatga atgtcatttt atttgcacca gaagatcttt ctttagtaaa
      601 aggcagcccg cagatcaggc ggaagttttt agatatggaa ttaggccaga tcaatccaat
      661 ctatctttat gatcttgtcc aataccaagc aattttgaaa caaagaaacc agtatttaaa
      721 gcaattgaat gaaaaaaaac aaactgattt gctttatttg gatattttgt ccgaacaact
      781 agcagcgttt ggcagcaaag tattgaaagc aagagcacag ttcgtcaagc gattggagtt
      841 ttgggctaat tctttgcatc agcaaatcac ccatcaaaaa gagcagctgg aaatcgagta
      901 tctcaccgca gtcgattctt tagaaacaca tacgcaagag caaatccaag aacaatttct
      961 cgcacttttg aaccaaaata aaaagaaaga cctttttcgg ggaacaacga cagttggtcc
     1021 tcatcgtgac gatctttcct tttttattaa tcagaaaaat gtgcagactt atggatcgca
     1081 aggacagcaa agaacaactg cactaagtgt caaattagca gaaatcgatt tgattaaaga
     1141 agagacgggg gaatatccaa tccttttgtt agatgatgtg atgagcgagc tggatgatag
     1201 tcgtcaactc catctgctag aaacaatcga aggaaaagtt caaacgttct taacaacaac
     1261 gacacttgac catgtaaaaa ataaaatgac tgttgaacct gagatttttt atgttcaaca
     1321 agggaaacta aaaggagaag aaactgaatg acagaagaaa gaagtttagt agaacgcgct
     1381 aaagagtatg atgccagtca gattcaagta cttgaaggac ttgaagccgt tcgtaaacga
     1441 cctggcatgt atatcggttc gactagttct gagggtctgc atcaccttgt ttgggaaatt
     1501 gtcgataatt cgatcgatga agtattagca ggttttgcaa cgaaaatcca tgtgattatt
     1561 gaaaaagaca acagtatcac cgttattgac gacggtcggg ggatacctgt agatatccag
     1621 gcaaaaactg gacgtcctgc agttgaaacg gtctttactg ttttacatgc tggaggaaaa
     1681 ttcggcggtg gtgggtacaa agtttctggt ggacttcacg gagtaggatc ttctgttgtt
     1741 aacgcgcttt ctacacagct tgatgtcaaa gtatacaaag aaggaaatgt gtactatcaa
     1801 gaatatcgtc gtggagctgt agtggacgac ttaaagatta tcgaacaaac ggatcgccat
     1861 ggaacaacgg ttcactttac gccagaccct gaaattttta cagagacaac tgagtttgat
     1921 ttttcaaaat tggctacccg gatccgtgaa cttgctttct tgaaccgcgg gatgcgtata
     1981 tcgattgaag acaaacgaga agaagaacca gtcatcaatg aataccatta tgacggcggg
     2041 attaaaagtt atgtcgaata cttgaatgca aacaaaaccg tgatttttcc agaacctgta
     2101 tatttggaag gtgaacagca agatatcgca gttgaagtct ctatgcaata tacagacggc
     2161 taccattcca atatcatgag ttttgccaac aatatccata cgtatgaagg cggaacacat
     2221 gaatcaggat tcaagactgc tttaactcgt gtgataaatg attatgcccg aaaacaaaag
     2281 ctgatgaaag aaaatgacga caacttgacc ggtgaagacg tgcgagaagg attgacagct
     2341 gttatttcta tcaagcatcc tgaccctcaa ttcgaaggac aaacaaaaac aaaattgggc
     2401 aattccgaag tacggacagt cactgatcgg ttatttagcg aacatttcat gaaattcttg
     2461 ctggaaaacc caagtgtggg ccgtcaaatt gttgaaaaag gattactagc ttccagagct
     2521 cgtctagcag cgaaacgtgc gcgtgaagta acgagacgta aaggtgcttt agagattagt
     2581 aatctgccag gaaaattagc ggactgctct agcaacgatc ctgaaaaatg cgaattgttc
     2641 atcgtcgaag gagattcggc cggcggatca gctaagcaag gtcgtaatcg agagtttcaa
     2701 gccatcttgc ctattcgtgg gaaaatcttg aatgttgaaa aagcaagcat ggacaagatc
     2761 ttggcaaatg aagagatccg gtctttattt actgctatgg gtacagggtt tggagaagat
     2821 tttgatgtat cgaaagctcg ctaccacaaa ttagttatca tgactgatgc cgatgtcgac
     2881 ggcgcgcata tccgtacatt gctgctgact cttttttatc gttacatgcg tcctattgtt
     2941 gaagctggat acgtctatat tgctcagcca cctttatacg gggtaaaaca agggaagaat
     3001 attacgtatg tacaaccagg aaaaaatgca gaagaagagt tgaaacaagt cgttgcttca
     3061 cttcctgcct caccaaaacc aagcgttcag cgttataaag gacttggaga aatggatgat
     3121 catcaactat gggaaacaac tatggaccca agcaatcgga tgatggcccg cgtcagtgtg
     3181 gacgatgcta ttgcagcaga tcagatattt gaaatgttga tgggggatcg agtagaaccc
     3241 cgtcgtgcat tcatcgagga aaatgcacac tatgtgaaga acttagatat ttaatcatga
     3301 aggaggaaaa agaatgagtg aagaaatcag agaaaacatc catgatgtca atctaaccag
     3361 cgaaatgaaa gactccttca tcgattatgc gatgagtgtt attgtcgcac gtgcacttcc
     3421 tgatgtcaga gatggtctga aacccgttca tcgccgtatt ttatacggca tgaacgaatt
     3481 aggtgtaaca ccggataaag cccataaaaa atctgccaga atcgtcggag atgtcatggg
     3541 taaataccat cctcatggag actatgcgat ctatgagtca atggtacgga tggcacagcc
     3601 attcagctac cgttatatgc tagtggatgg tcatggaaac tttggttctg ttgacggtga
     3661 cggtgccgcc gctatgcgtt ataccgaagc acggatgagc aaaatcgcta cagaaatgct
     3721 gcgtgatatc aataagaaca ctgttgattt ccaaagcaac tatgatgata cagaaaaaga
     3781 accagttgta ctacctgctc gcttcccgaa tcttttagtg aacggaacaa cggggatcgc
     3841 cgtaggtatg gccacgaata tcccacctca taacttgtct gaggtagtag cggctatcga
     3901 tttgctgatg gataatcctg atgtgacgac caatgaattg atggaagtat tacctggacc
     3961 agattttcca acaggtggtt tggttatggg aaaatcagga atccgaagag cgtatgaaac
     4021 aggtaagggt tctattaccg tacgtgcaaa agttgagatt actgagatgc caaatggtaa
     4081 agaacggatc atcgtgacag aattgcctta tatggtcaat aaagcaaaat tgatcgaacg
     4141 gatttctgaa ctgcacagag acaagcgtat cgaagggatc acagatttgc gggacgaatc
     4201 ttcccgtgaa ggtatgcgga tcgtcattga catccgtcgt gacgccagcg catctgttat
     4261 cttgaataac ttatataaat tgacatcctt gcaaaattca tttggattca atatgttggc
     4321 gatcgaaaaa ggtgtgccga aagttttgag cttgaaacaa attcttgaaa actatatcga
     4381 acatcagcgc gaagtcatca caagacggac agtctttgaa aaagaaaaag cagaagcaag
     4441 ggcacacatt ttagaaggtt tgcgtattgc tttggatcat atcgatgaaa tcattgcgat
     4501 tatccgcggg tctaaatcag atgacgaagc aaaagcgacg atgatcgaac ggttcgattt
     4561 atccgatcgc cagtctcaag ctattttaga tatgcgttta cgccgattga ccggtttgga
     4621 acgggaaaaa atcgaaaatg aataccaaga actcttaaaa ctgattgaag atttaactga
     4681 cattctggca agaccagaac gtgttacaga aatcatcaag aatgaattag cagacttgaa
     4741 tcaacgattt ggtgataaac gacgcacaga attactagtc ggtgaggtct taagtctgga
     4801 agatgaagat ttaatcgaag aagaagaaat cgttattaca ttgacaaaca atggttacat
     4861 caaacgtttg gcaaataatg aattcagagc tcaacgaagg ggtggacgtg gtgtccaagg
     4921 aatgggtatc cacaatgacg acttcgtcaa aaatctggtt tcttgttcta cccatgatac
     4981 attgttattc tttacgaata acggaaaagt gtatcgggca aaaggatacg aaattccaga
     5041 atacgggcgc acagccaaag gaataccaat tatcaattta ctaggtatcg attcgagtga
     5101 aagcatccag gcaatcattg cggtgacggg agaagcagaa gaaggccact atctcttctt
     5161 tacgacgcgt caaggtacag taaaacgtac ttctgtcact gcttttgcta acatccgaag
     5221 taatggtctg atcgcaattg gtttgaaaga agacgatgag ctcgtcaatg tgctactgac
     5281 agatggtcaa tctaatatca ttattggaac acataacggc tactctgtca ctttcaaaga
     5341 agaagcagtc cgcgatatgg gacgtacagc ttccggggtt agaggaatac gcctgcgtga
     5401 ggaagattat gtcgttgggg cagccttaat gaaggaagat caagaagtat tagtgatcac
     5461 agaaaaaggt tacggaaaac ggacaaaagt atcggaatat ccagtgaaag gccgcggagg
     5521 taaagggatc aaaacggcga acatcactga aaaaaatggt ccactagctg gattgaccac
     5581 tgtttctgga gatgaagaca tcatgctgat tacagataaa ggtgtcatta tccgtttcaa
     5641 cgtatcgact gtttctcaaa ctggacgttc aacattaggc gtacgtttga tgaagatgga
     5701 agctgatacg aaagttgtca cgatggcagc agtcgaacca gaagctgcag aacaaacagt
     5761 tgaagaacaa ggatcagaag aatagccatc aagactaatt gtgtaagcta gcaagaatga
     5821 ccgattaaaa aatctggtta ttcctgctgg cttttttgat tagttgaatt tctcttaaaa
     5881 tattcgaaac tgttcattct gtattgcttt tgaccaaaat agagtgtata atcagagctt
     5941 gtgagtagtc taattaatta gataactctc cttgctctta ggaacctaag agccgaaagt
     6001 ccacagggag gtgaatatca atggaaaaca cgaaatatga aatcatgtac attattcgtc
     6061 caaacattga tgaagaagca aaaactgctt taatcgaacg tttcgatgca atcttgaaag
     6121 ataacggagc agaagttatc gaatcaaaag attgggaaaa acgccgtttc gcttatgaaa
     6181 tgaacggtta ccgtgaaggt atctatcaca tcgttaaagt atcttctcca tcatcagcaa
     6241 atgctgtcaa tgaatttgac cgtcttgcta aaatcaacga cgatatcatt cgtcacatga
     6301 ttgttaaaga agaagcttaa ttgttgtttc atgtgaaaca atagaaaacg atgagaggag
     6361 atgactattt tgattaataa tgttgtatta gttggacgtc tgacgaaaga tccagatttg
     6421 cgttacacag caagtggtac ggcagtcgca acatttactt tggctgtgaa tcgtaatttt
     6481 acaaaccaaa gcggcaatcg cgaggctgat tttatcaact gtgtgatttg gcgcaaatca
     6541 gcagaaactt tagcaaacta tgctcgtaaa ggaacacttt taggtgttac tggacgtatc
     6601 caaactcgtt cttatgataa tcagcaagga caacgtgtat acgtgacaga agtagtagct
     6661 gacaacttcc aattgctgga aagtcgttct gcatctgaaa atcgccaaca aagcggcgga
     6721 ttccaaagtt ctggtcaatc agctggcgga tttggcggaa acaataactc taatcaaaca
     6781 tctcaatcat ccaacggtat gcctgatttc gaccgtgata cttctgaccc attcggctca
     6841 agttcaacga tcgacatttc agacgatgat ttaccattct aaacgaattt gataggaggg
     6901 aaatagaatg gcacaacaaa gaagaggcgg acgtaaacgt cgtaaagtag actacattgc
     6961 tgctaaccat attgaatata tcgattataa agatatcgaa ttactaaaaa gatttatttc
     7021 agaacgcggt aaaatcttgc cacgtcgtgt aactggtaca ggcgctaaaa accaacgtaa
     7081 aattactgtt gcaatcaaac gcgctcgtat catgggcttg ctacctttcg ttagcgaaga
     7141 ctaataaatc ctaggaattg aatgactgat ccttttgatc agctattcaa ttcctttttt
     7201 tctgtaaaaa taacctctat tcgtttagtc gctcgaatga cgaaagttat ggtaaaatca
     7261 aattagtcat atatgggagg aaaattatgg aaaataaaaa caatcattta tccccgaaac
     7321 ctctcttcct tatcctgttt atattaatcg aagtattggt agtcttaatt ttaccatttc
     7381 gttttgttag cgcttcggtc attctattga tgaatttatg gggtgttcgc tattggttga
     7441 aaatgaaacg ttggcttatt ttaaataaac gtgaaaaaat ccagcaggct tcgactgtag
     7501 ctgaaaccaa tctagcttat gtctcagaag tcatgcctgt aggagtgatt gcttatcagc
     7561 catcaagcca aaaaatcgaa tggctcaatc cattagcatt gaaaatcaaa gatcaaagtg
     7621 gattaacaga ggaagaattg ctagatactt tttttactgc aaaagaaaaa aagaaaaacc
     7681 aagtctatct agcagataaa acatttcagt ttgatatgga tacagtaaag ggtgttgttt
     7741 attttaatga tattacccaa aaccagttgt taaaactcca tcagacgaac agtcagccgg
     7801 tgattggtat tttatctatt gataactatg cagatgccat tgataaattg gatgacaaag
     7861 agatttctta tttgaactct ttcgtcacta cactagtttc tgactggatg aatgaatacc
     7921 atgtcttttt caaaagaatc aacgcagaac ggtttttctt cgtagcacag atgatcgatc
     7981 tgaaaaagat gatggataaa aaatttgatt tgctggatcg ctttcgagca gaagctgaaa
     8041 aacagagctc gcctatcacc cttagtatgg ggattgctta tggctcaaga accctcgatg
     8101 aaatcggtgg agaagctcag aataatttag atgttgcttt agtccgtgga ggcgaccaag
     8161 ttgtcgtgaa agaggtaaaa gaaggagcaa aacctgttta ttatgggggg aaatctgcta
     8221 agaatgtcaa acgcacgcgt gttcgttctc gagcgatgag taccgctttg cgtcggattt
     8281 ttgatgaaac aggcgatgtg tttatcatgg ggcacaagtt tccggatatg gatgctttag
     8341 gctctgcttt tggtgtcagt tgtctggcac gcttcaacca gaaaaaagct tacatcgtta
     8401 tcaacgagaa cgagatcatt ccagatgtag aacggtgtct tgaagaaatc cataagcatc
     8461 ctgaattaga aatgcaacta atctctccag aacgtgccat agagctgaaa aaagaagagg
     8521 acctcctcgt gatggtggat taccataaac cttctctatc gatctctcag ggcctatatg
     8581 agcaatttga taagattgtg atcattgatc accatcgtcg tggggaagag tttccaagta
     8641 aacctctgct gacttatatc gaatcttcag cttcttcagc ttcagagcta gtttctgagc
     8701 tgatccaata ccaaagcagc aaaacaaatc gcttagatcg ttttgaggcc acattattgc
     8761 ttgcagggat ggttgtagat acaaatagtt tcaatgtccg tacaactgca cgaacttttg
     8821 acgttgccag ctatttaagg tcttgtggag cagacgcatc cttagtacaa tatttattga
     8881 gctcggattt agcgacatac ttggagatca gcaatttgat tgctggaagc gactatgtga
     8941 cttctgatat tgttgtggcg gcagggacag acgaaaaaga gtataatagt gttatagcag
     9001 caaaaacagc agatacattg ctttcgatga tcaatatcaa tgcagctttc gtcattacaa
     9061 agcggacaga cggcttgatt gggatcagtg ccagaagtac cggcacgatc aacgtccaaa
     9121 tcatcatgga atccttaggt ggcggaggac acttcacaaa cgcagctact caactggaaa
     9181 atacgacagt tgaggaaaca aaacagaagc tatttgctgc aatcaaagaa aatatgaatc
     9241 aaatatatga taaggagtaa tccaaatgaa agtaattttt ttacaagatg taaaaggaaa
     9301 aggtaaaaaa ggggacatca aagaagttcc agcaggttat gcacaaaatt atctaatcaa
     9361 aaataacttg gcaaaagaag caacgaaaag cagtatcagt cagttagcag gacaaaagaa
     9421 agcacaagaa aaacacgaag ctgaaatctt ggaagaagct aaaaagttaa aagaattctt
     9481 ggaaaaagaa gatactgtag tcgaattgaa ggcgaaagca ggggaagaca gccgtttgtt
     9541 tggttcgatc ccttcaaaac aaattgctga agggttaaac aaacaataca aaatcaaatt
     9601 agataaacga aaaatcgaat tagaaaatcc gatccgttca ctaggctaca caaacgtacc
     9661 tgttaagctt catcatgagg tgacggctac gatcaaagtt catgtcgtac aagaataaca
     9721 aatcttgata gcgatgttca gctggtaagt ataaccagaa ggaaaaaaga caaaccgaaa
     9781 tttatctgag aaaacagctg cagcaatgaa agcaaaagct gcaaatcgat ttcagattga
     9841 tttcggtttc ttttttggga acagcaaaat agcataaaga aaacgagtat aataaaagag
     9901 aactattttt ttattacact ttcggtacga atgatgactt ttctctcatt ttcttgtaaa
     9961 atgaagcaat tgaagacaag gagcgatacg tatgaatgaa gtgtggcagg atcgaatccc
    10021 accgcaaaat attgaagctg aacaagcggt tttaggctct gtttttcttg acgcagaaac
    10081 gatcattgat gccatggagt acatagagcc aaaagacttt tatcgtcgtg gccatcaaat
    10141 tatttttgag acaatgatcg agttaaatga tcgtaatgaa gcgattgacg tggttactgt
    10201 caaagaccgg ctggaacaag ccaatttgtt agaagatgcc ggtggtttaa gttatctctc
    10261 agacttggca ttagcagttc cgacagcagc aaatatcgtc tactatgcga aaatcgttga
    10321 agaaaaatca ttgctgcgta cattgatcca gacagcaaca ggaatcgtta cgaaaggatt
    10381 cgagcaagga gaagaagttc aaacaatttt agatgatgcc gaacgcagta tcttggaggt
    10441 ttcggaaaaa agaaatcgaa gcggcttttt atctattgct gaagtactga actccacgat
    10501 tgaaaatatc gatcgactag ctcaaaacaa tgaagaaatc acaggtctac caactggcta
    10561 tcaaggtctg gataaaatga cagcaggttt ccagcccgaa gaactaatca ttcttgctgc
    10621 acgtccagcg gtaggtaaaa cagcgtttgc attgaatatt gctcaaaatg taggaacaaa
    10681 aacagatcgt tctgttgcga tatttagttt ggaaatgggg gcagaatctc ttgtaaatcg
    10741 gatgctttgt gcagaaggtt cgattgaggc cagccattta cgtacaggtc agctttcgga
    10801 agaagaatgg tcgaatctga tcgttgcgat ggggagtctt tctcgagcaa atatttttat
    10861 cgatgataca ccagggatca aaatctctga aatccgtgcg aaatgtcgga aactagcgca
    10921 agaaaaaggc aatctaggct tgatattgat cgattatttg cagttgattg aaggtaacgg
    10981 taaagagaac cgtcaacaag aagtatcaga tatctctcgc caactgaaaa aactggcgaa
    11041 agagctgaaa gtacctgtca ttgctctttc acagttatct cgtggagtag aacagcgaca
    11101 agacaaacga ccagtgctaa gtgatattcg tgaatccgga tcaattgaac aagatgcgga
    11161 catcgtagcc ttcttgtatc gagatgatta ttatgaaaga gaaggcggag atgacgatga
    11221 tggaaacgag ccaaaaaatg acaacgtcat cgaagtcatc atcgagaaga accgaagcgg
    11281 tgcacgagga acagttgaac tactgtttat caaagaatac aataagtttt cgtcgctttc
    11341 gccaagagaa gaattttaaa gaagaggttg tgagccttga gcaatcccga gagctctcgt
    11401 aagaagatgc aaggagtaac cgcaggaacg actagagtac ttaatttaaa cagatctcca
    11461 aagggattgt gacaaaagtc ttgtcacagt ccctttttcc gaataaacgg tgttcaatca
    11521 gctgaccctt ggtattagct cagcaaacgt caaaatatga ggaactattt tgacgtttgt
    11581 ttctctaata ctcaggtcaa aacgctgatc ttacgacctc ttttccttaa acggtgttct
    11641 cctgccaact cctcggtcac aagtcacaat atcatcaaac atgagaagca gttttttgaa
    11701 attgctcctt gtgactcaga gttaaacggc aggctcacaa cctctttctt aaacggtgtt
    11761 caaaagcttg catctgcggt aatacgccca atcaaacgaa aaatatgaga agctattttt
    11821 gtttggctgt tcttattact tgatgcagga cagctttttc acaacctctg attcacggtg
    11881 ttccaaaagt agcttcttcg aaaaataagt cgaaatttca caaaaatttg agaagcaatt
    11941 ttcgtacatt ccttcttatt tcttgaagct gaccacttct gttacaacct cttttttaat
    12001 aaccattcta aaaagataaa gttcgttttt taagcgtttt tataaaataa aaataaaaat
    12061 cattcgattt tatttgaaat atgtatctat tattgataca atggttcagg attaaataat
    12121 atagaacgag gtgttcacat gtcatctgta gtagtagtag gtacgcaatg gggcgatgaa
    12181 gggaaaggaa aaatcacaga ctttctcagt gaaaatgctg aagtgattgc acggtaccaa
    12241 ggtggcgaca atgcagggca cacaattaag tttaacggtg tgacttataa actgcatttg
    12301 attccttctg ggatttttta taaagacaag atcagtgtga tcggaaacgg cgttgtagtc
    12361 aatcctaaat cattggttaa agaattagct tatttacatg aaaaaggtgt agaaacaagt
    12421 aatttgcgta tttctgaccg cgcgcacgta atcttgcctt atcatatcaa attggaccaa
    12481 ttacaagaag atgctaaggg cgaaaataag attggtacaa cgattaaagg aatcggacct
    12541 gcctatatgg ataaagcagc tcgtgtaggt atccgtattg cagacttatt agataaagaa
    12601 atttttgaag aacgtcttcg tttgaatcta gatgacaaaa accgtcaatt tgtaaaaatg
    12661 tttgattcag aaccacttga ttttgacgat atttttgaag aatattatga atacggacaa
    12721 caaatcaaac aatacgtcac agatacttcc gttatcttga atgatgcttt ggatgcaggc
    12781 aaacgcgtgt tgtttgaagg cgcacaaggt gtaatgctgg atatcgacca gggaacttat
    12841 ccttttgtta cttcttctaa cccagtagcc ggtggggtaa caatcggaag cggtgtaggg
    12901 ccttcaaaaa tcaataaagt tgtcggcgtc tgcaaagcct atacgtcacg tgtcggtgat
    12961 ggaccatttc caactgaatt atttgatgaa acaggagaac aaatccgcga agtaggacga
    13021 gaatacggta cgacaactgg tcgtccgcgt cgtgtaggct ggtttgattc tgtagttatg
    13081 cgccattcta aacgagtttc aggtattacc aacctatctt taaactcgat cgatgtatta
    13141 agtggattgg aaacagtaaa aatttgtacg gcttatgaat tagatggcga actaatctac
    13201 cactatccag caagcttgaa agagttgaac cgctgcaaac caatctatga agaattgcca
    13261 ggctggtcag aagacattac aggatgcaaa acacttgcag aactgccaga aaacgcacgt
    13321 aactatgtac gtcgtatctc agagcttgtt ggtgtacgta tctcaacatt ttctgtcgga
    13381 cctgatagaa cacaaacaaa catcttagaa agcgtctggg cgcaaatcta aggataaaaa
    13441 cgacgactga atcaggacgt tcttaccaga gcgagtcgaa gtgtttatct atacgataat
    13501 aataacttga agaggtagtg aaagagacgt ttagccttat tgccgatagg ctgtctcttg
    13561 aacactgttt attcagaata agggactgtg acaaaaaccg tgtcacagtc cctttttccg
    13621 aa
//
LOCUS       DAZULG010000075        13212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000075 DAZULG010000000
VERSION     DAZULG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.077"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG9CY_001968"
     CDS             147..1016
                     /locus_tag="ACG9CY_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4938470.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG9CY_001969"
     CDS             1225..2250
                     /locus_tag="ACG9CY_001969"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4938471.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG9CY_001970"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG9CY_001970"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS4938472.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACG9CY_001971"
     CDS             3333..5036
                     /locus_tag="ACG9CY_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4938473.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG9CY_001972"
     CDS             5038..7071
                     /locus_tag="ACG9CY_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS4938474.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG9CY_001973"
     CDS             7082..7522
                     /locus_tag="ACG9CY_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4938475.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG9CY_001974"
     CDS             complement(7722..8849)
                     /locus_tag="ACG9CY_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4938476.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG9CY_001975"
     CDS             complement(8852..9529)
                     /locus_tag="ACG9CY_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4938477.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACG9CY_001976"
     CDS             9793..11559
                     /locus_tag="ACG9CY_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS4938478.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACG9CY_001977"
     CDS             11574..12011
                     /locus_tag="ACG9CY_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4938479.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACG9CY_001978"
     CDS             12024..13175
                     /locus_tag="ACG9CY_001978"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS4938480.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAZULG010000076        13082 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000076 DAZULG010000000
VERSION     DAZULG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.078"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..2196)
                     /locus_tag="ACG9CY_001979"
     CDS             complement(76..2196)
                     /locus_tag="ACG9CY_001979"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4938481.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2426..3604
                     /locus_tag="ACG9CY_001980"
     CDS             2426..3604
                     /locus_tag="ACG9CY_001980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS4938482.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3620..4378
                     /gene="aroD"
                     /locus_tag="ACG9CY_001981"
     CDS             3620..4378
                     /gene="aroD"
                     /locus_tag="ACG9CY_001981"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS4938483.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4401..4886
                     /gene="ybaK"
                     /locus_tag="ACG9CY_001982"
     CDS             4401..4886
                     /gene="ybaK"
                     /locus_tag="ACG9CY_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS4938484.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4957..6210)
                     /locus_tag="ACG9CY_001983"
     CDS             complement(4957..6210)
                     /locus_tag="ACG9CY_001983"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4938485.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6326..7675
                     /gene="gdhA"
                     /locus_tag="ACG9CY_001984"
     CDS             6326..7675
                     /gene="gdhA"
                     /locus_tag="ACG9CY_001984"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS4938486.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7787..9133
                     /locus_tag="ACG9CY_001985"
     CDS             7787..9133
                     /locus_tag="ACG9CY_001985"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS4938487.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9441..9827)
                     /locus_tag="ACG9CY_001986"
     CDS             complement(9441..9827)
                     /locus_tag="ACG9CY_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4938488.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10095..10244)
                     /locus_tag="ACG9CY_001987"
     CDS             complement(10095..10244)
                     /locus_tag="ACG9CY_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938489.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10706..10918
                     /locus_tag="ACG9CY_001988"
     CDS             10706..10918
                     /locus_tag="ACG9CY_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938490.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11196..12995)
                     /locus_tag="ACG9CY_001989"
     CDS             complement(11196..12995)
                     /locus_tag="ACG9CY_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS4938491.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4290 a         2353 c         2577 g         3862 t
ORIGIN      
        1 ttctaagatt tctctttacc aacatcattt gacaaagagc ctaattttgt cgctgctcct
       61 tttaaattta ttttatcatt cggaagaact tgtggtcgtt ccgtgaagtt cttggtacgt
      121 ttgcgtttta taaagatcaa cagttgattg tcccttgtgt tcactaaaga cactagttga
      181 cttgtcacca agagattttt cagcttcttt taatttctct aagccattct tgtagtcata
      241 atcactggta tcaattgggt ctaatccact attcgtatag aaccgaagaa gatcaccatt
      301 attgatttgg tcagaaacag ctaactgttt atcaactttt tctttcagtg aatcaatttc
      361 attctgtact tcctcagtag gttcagtaat agcttcacca gtttcgttac tatagatcgt
      421 accaccgtag taagtatatt ttggtgtaac aaaatcacca tcacggaaag aaacaagctg
      481 ttcatgatct ttcgagaata gatcttgtcc taactgaata taattttttg tatctacacc
      541 tagtaagtga agtaatgtag gcaaggcatc aacttgtccg ccgtaagtgt gattgattcc
      601 gccctgtgtt tgtccaggga tatggatcat ataagggaca cgctgcattt gtgcattgtc
      661 gtagtttgtc caagtagaac ttgtttttcc aactaattca gctagatttt tatttcttga
      721 attcgagaca ccgtaatggt caccgtaaag gacgatgact gaattatcat acaagccgct
      781 ttctttcaaa taattgaaga attcttgtac ggcggtatct aaataattgg ctgtcgcaaa
      841 gtaaccgttg atcgtttcat catttgtatt ggctaacggg aatcctgctt cgtcattggt
      901 gaattttgaa taaggataat ggttagatac agcaatgaat tttgaataaa atggttgttg
      961 cagacgttcc aagtattgta cggattgttg gaaaaatggt ttatcatgca acccgtattg
     1021 gaacgaattg tcatcattaa catcataata agaagaatca aagaaataat catatcctaa
     1081 attcttgtat gtctcgtttc gattccagaa tgtaccagcg ttcccatgga ataccgcact
     1141 agtgtaacct tgtgtttgac ttaaaatatc aggtgcagct tggaaagtat tttttccgcc
     1201 taattgtgtg aacaaggagc cttgattcaa gccaaacaat gaattttcca tcagcgtttc
     1261 cgcatcgctt gtttttcctg cttttacttg atggaaaaag ttgtcaaaac tgaacgtgct
     1321 gttgctgtgg taaagactat tgatgaaagg tgtaacttca agctggagac cattctcatc
     1381 tgtcaattta taatcgatca ggaattgctg gaagctttct aaatggatat aaatcacatt
     1441 ttttccttta gcgagcccga acaaatcatc atttggtgct gcataatgtt cttttacata
     1501 attttctact tctttcatat cattgggact tgcttctgcc cgcacttggt ttgtctggta
     1561 agtttgaaca ccgtcataaa ccatgaaagc attcaagcca agatatttga ccaaataatc
     1621 acgagagaat gttcgagtca gcaattctgg acgatcagct tccgccataa aaagatttcc
     1681 tgagaaaatc ataactgcta atgtagagat cgcaaaagcc attcttgctc ggatcgggcg
     1741 agggtccaac ttgatttttt ttgcaatcag cagaacagtc aagataataa aatcgatcca
     1801 atagatcaca tcatatgggc gaaacagtcg aatggcactt tctcccagac cactcgctac
     1861 tttcccagca cctaaaatcg tgttgatcgt aatgaagtcc gtgaattctc gataataagt
     1921 gacattcgaa aaaacaagca atgtcaaaag aaaataaatg accatcatcg tgatataagc
     1981 aactctaggg cgtctgatat aaagagcaat tccaagtaat aacaatgtag ttgcaatagg
     2041 gttgataaac aagataaagt attgaatgcc actttcgatt ccgagatgga aatcaactaa
     2101 ataggcaaaa atcgttttca accaaagtaa aacaactagt aaagaaaaaa atccgagcct
     2161 cttattcaaa aaggcaggtg tttttataca tttcaagata atcttccttt ctgcacttct
     2221 taatcacaga agtgtcatga aaccatgtta cacagctcac ttattctcat ttattttact
     2281 agtatgacaa tatagtgtca attgaaacca gaagtttatg ctgtttcatt aaggttttct
     2341 ttaagcttca tgaaaaaatg agcagtaaga caagtcctat gttttttgtt atacttggga
     2401 agagaaagct aaagcaggtg aaggaatgaa agtaaaagta aaaaaaagag caagcaatcg
     2461 attgaaaaaa ggatatccac tgatccaaaa agaagacctt gctcatcccc aaaaagaaac
     2521 cacgcaatgg gtggaattag ttgattcgca aaaccaattt atcgcaaaag gatatctagg
     2581 aaagcagaat aagggaatcg gttgggtatt gagccagcag aacatcccat ttgatcaatc
     2641 tttttttgaa gaattttttg tgaagtcaaa agaaaaaaga actttctttt tttcagatga
     2701 ttctactacc gcttttcgta tattcaatgg agaaggagac gggatcggcg gtctgattgt
     2761 tgattattat gcaggttatg ctgttttttc ttggtataac gaaacgttat atgcacatcg
     2821 tgaattattg attcaagctt ttcgtgaaat ctacccggaa atcgtaggag gctatgagaa
     2881 aattcgattt gaatcagtaa atctgcctga gagccagttc atttacggaa aagaggcacc
     2941 ggaacccttg atcgtaaaag aaaacggagt aaaatttgcc gtttatttaa atgaaggatt
     3001 aatgactggg atctttttag accaaaaaga agtacgggga tcattagcag aaggtttcgc
     3061 ttctgggaaa acagttttga acatgtttag ttataccggc gccttttctg ttgctgccgc
     3121 aatgggaggg gcaatcgaaa caacaagtgt cgatctagca aaacgcagtt taaaaaaaac
     3181 acaggaacag tttgaagtga atggtttgga tgtcgatcag caaaaaatca ttgttatgga
     3241 cgtattcgag tatttcaaat acgccaaaag aaaacaattt ttatatgaca tgattgtatt
     3301 ggatccacca agttttgcca gaaataagaa aaaagtcttt cgcgtagcaa aagattatgg
     3361 ggaactggtc aaggatagtc tggatatttt agcagaagac gggatactta ttgcgtctac
     3421 aaatgctgcg aatgtctcga tagaacaatt ccaaacaatg attgaagagg aattccatgc
     3481 caaaaatgtt tcttttgaac aacttgcttt atatcgtctg ccaaaagatt tccagacgac
     3541 ggataccttt ttagaaggca attatctgaa agtgtttgtt tatcaaatta aaaataacca
     3601 ttagaaggag cgatgcagaa tggtttgggt aaagggattg aatataggaa gcggtagacc
     3661 aaagatttgt gcgcctctta tcggggccgt ccgagaggaa gtattacgtg aagcagttct
     3721 tgcacgtgaa gcaggggccg atttagtcga atggcgagtc gatcattatc gtgaagtttt
     3781 ttcttttgat gaagtagcag aaacattggt tcttatccat gagatgcttg atggactgcc
     3841 gttattattt acgtttcgta ccttagctga aggcggagaa caagacatat cgatccgtca
     3901 gtatcgcgac ctttatgaac atgtgataca cacaggatta gtagatatgg tcgatatcga
     3961 gttgttcaaa gtagaaagtc taggaaaaag actgatagag gaaatcaagt ctttaaacat
     4021 tcctttaatt atcagtagtc atgattttaa agaaacacca gcagaccctg tcttgcttta
     4081 ccgattgaat gttatggaac attttggagc cgatatcgga aaactggcag taactcccaa
     4141 taatgaacga gatgttttgc ggttgatgga gttgactaga cgggcaaatg cttttgtctc
     4201 catgcctatt attacgatgt ccatgggaaa tcttggtaaa atttctcgtc tggctggcgg
     4261 aacgacgggt tccgttatga catttggttc cttaagcagc gattctactt ctgctccagg
     4321 acaattaccg gtcaaagcat tgaagcaaat tattcaatta ttaaatgaac cgttgtagaa
     4381 caagtttgag gtgatatttt ttggcaaaga aaaaaatagt aaaaacgaat gcaatccgta
     4441 tggtcgaaca aaaaaagatt ccctatacag agcacgaata tgagtgggac gaaagccacc
     4501 taagtgcgtc atcggtcgct gaacagcttc cagaaagcca atctcatata tttaaaacac
     4561 tggttgctgt gggcaatgtg acggggcctc ttgttgctgt gataccggga gaagccgaat
     4621 tgaatctaaa aaaattagca aaagtttcag ggaataaaaa agtagaaatg ctccatttaa
     4681 aagatttgga agcaacgacc ggatacatac gtggtggctg ctcgcctata ggtatgaaaa
     4741 aactatttcc tacttattta gatcaaattg cagaaagcta tgaacaaatc attgtctcgg
     4801 ctggcagaag agggctacag atggagttag cacctcaaga tatctgtgca ttaacgagcg
     4861 gacaatttgc agatataaaa caataaatca tacgtataaa agaaagaagg aggccgggat
     4921 gttgtttgtc cagtctcctt ctttcttttt tatacattat gctctttgcc cggcattcat
     4981 tcctgctaca tgccctgtag cgaaagccgc tgtgatatta aagccgccag tataaccatt
     5041 gacatccagt aattctcccg caaaatacag accatcaatg attttactct ccaatgtttt
     5101 aggataaatt tcctttagag aaacccctcc gccagttaca aatgatttct caataggaaa
     5161 agttttgttg attgatagct taaactcttt gcatagttga acaaaatctt gaatttgttt
     5221 ttgagaggct tgttgtcctg tcagatgatc catttcaagc cgctcaagaa agaaaactaa
     5281 caatcgctca ggcaatatac catgccaagc attgaccaag ttctttttgg ttgctttact
     5341 tttttctgtc aatatatgaa taagctcttg tttggtttgt gtagggaaac aatccagaga
     5401 aagtgtcact ggttctcccg ttttctctaa ttctttattg acaaaacttg aacaacgcag
     5461 tgcagccgga ccagaaatac cgaaatgggt gaaaagtaaa tccatcgtat gttcagtcaa
     5521 tacacgacct tttttattta aaacgcgtaa agtgatgtct tgtaaagaaa gcccttgaag
     5581 tgttttctcc tgaatatacg gttcatccga tatcaagggg gactctgtcg cataaagagg
     5641 cgtcaccgtg tgacccactc gcttcacgat cttgtaccca tctccagtag aaccagtaga
     5701 tggataagtc cgtccaccag ttgttaagat aatggcagga gcatggaact cttcgaattc
     5761 cgtgcgtata ccgtaaattt tatgttcttt gtggatcaat ttctctactc gagtagagaa
     5821 tagaagcgtc acatctagtt ctttcagacg attgagcaat gcttcgataa tcgtttttga
     5881 tttatttgtt acaggaaaca tccgtccatg atcctcttct tttaaatgta cgccttgtga
     5941 ttcaaagaag ttcatgatat cgaaattatt ccattgcgca aacgtactat ataaaaactt
     6001 tccatttcca ggaatatgtg caatcaaatc atcaacagga cgattgttcg taacattgca
     6061 ccgaccacca cctgtcatca gtagtttttt tcctgctttt ttgttttttt cgatcaagag
     6121 tacgcgtgcg ccttgttcag ctgcactgat cgctgccatc atcccgcttg ttccagcgcc
     6181 gactacgatc acatcataca gtttttccat tccctcacct caaggaaagt gtagcatatc
     6241 tgaagaaaga aaacagaaaa aatgttttga gccaaaagag aaaatgagct aaaatgaggg
     6301 aagataagca aaggagcgtg gaacgatgac aaacgcaaca gaatatgttc aagcaattca
     6361 agaaaagcta catcaaaagg atcaagggca agaagaattt ttacaagcaa tcgatgaatt
     6421 catgcccacg gttatgtctt ttttggacac tcatcccgaa tatatcgaaa agaatatctt
     6481 agaactttta acggaaccag aaagagttat tcaatttcga gtgccttggc aagatgatga
     6541 aggaaactgg cgagtaaata gagggtaccg tatccaatac aactcagcta tcggccctta
     6601 taaaggtgga ttgcgcttcc atcctagtgt caatctaagt atcttgaaat ttttggcttt
     6661 tgaacagatt ttcaagaata gtttaaccgg tctgccaatc ggtggaggaa aaggcggaag
     6721 tgacttcgat cctaaaggaa aatctgataa tgagatcatg cgtttttgtc aaagtttcat
     6781 gctggaattg tcaaaacata tcggcccatc gattgatgtt cctgcgggag atatcggtgt
     6841 aggagctaga gaaatcggct atttgttcgg cgaatataaa cgattgaaaa aatatgatac
     6901 tggtgtgttg acaggtaaac ctttggattt ttggggaagt aagatacgta cggaagctac
     6961 tggttatgga ttagtgtatt atgtcaaaca tctattgaac gaagaaaaag attcatttaa
     7021 tcaaaaaaca gtttttgttt ccggaagcgg gaatgtagcc atttatgcaa tcgaaaaagt
     7081 gcaagagcta ggcgggaaag ttgttacatg ttcagattct agcgggtaca tctatgatcc
     7141 agaaggaata gatgtgaaac tattgaaaga agtcaaagaa atcaatagac aacgtttgac
     7201 ggaatacgca aaccaacgtc cttctgctat ttatcatgct ggagaatctg tttggacatt
     7261 gaaagaaaaa gcggatatcg ctttgccatg tgctactcaa aatgaaatag acgaaagctt
     7321 ggccgaaatc ttagtagaaa atgggatcaa aatcgtagca gaaggcgcta atatgccatg
     7381 tacagtaaaa gctgtttctg ttcttcatga agcaggcgtg tggtattgtc caggaaaagc
     7441 tgctaatgct ggaggagtag cagtatctgc tttagaaatg agtcagaacg cccagcgatt
     7501 gacttgggaa agagagcaag tggatgagca gctggatcaa atcatggaaa cgatttacca
     7561 tacgtgtcgc caaacagcag ccgagtacgg cgacgaaaaa aatctgcttc ttggagcaaa
     7621 tgttgctgga tttgaaaaag ttgccaaagc aatggcagca gaaggtttag tttgaaaaat
     7681 gaaactttct tggaaatatt gtcagcattt tgtttatttg cttgaaaaat aataaaaaaa
     7741 aacgtaaagt attacctgta gagtaaacct ataaggagga gaacaaatgg cacacattca
     7801 ttttgactat tcaaaagtag caccgtttgt caacgaacaa gagttagggt acatgcaaag
     7861 ccaagtaaca gcagcccaca acgaattacg ggaaggtact ggcgcaggaa gcgacttccg
     7921 cggctggatc gatttaccaa cgaactatga caaagaagaa tttgcacgta tcaaagaagc
     7981 agctaaaaaa atccaatctg attctgaagt attagttgtt atcggtatcg gtggttctta
     8041 tttaggcgct cgtgcagcga tcgacttctt gaatcacaca ttcttcaatt tattagatag
     8101 caacacaaga aaagcaccac aaatcttctt tgccggaaat tcaatcagct caacatatat
     8161 tgctgacttg atcgaagtaa tcggtgatcg cgacttttca gtaaacgtta tttcaaaatc
     8221 tggaacaact actgaaccag cgattgcgtt ccgtgtattc aaagagcttc ttgttaagaa
     8281 atatggacaa gaagaagcaa acaaacgtat ctacgctaca acagataaag caaaaggtgc
     8341 agttaaagta gaagcagatg cagaaggttg ggaaactttc gtgattcctg atgacatcgg
     8401 cggacgtttc tctgtattaa ctgctgttgg tttgttacca atcgctgtta gtggtgcaga
     8461 tatcgatgca ttgatgcaag gtgccgctga tgcaagcaaa gcatactcaa gcgacaaact
     8521 agaagaaaac gaagcttacc aatacgcagc aatgcgcaac attctttacc gtaaaggcaa
     8581 agtaactgag ttgttgatca actatgaacc aggaatgcaa tacttctcag aatggtggaa
     8641 acaattgtat ggtgaatctg aaggaaaaga ccaaaaaggc atctatcctt caagcgcaaa
     8701 cttctcaact gatttgcact ctttaggaca attcatccaa gaaggacgtc gcaatatttt
     8761 tgaaacagtt gtgaaggttg aaaaacctcg taaatcaatc acaattcctg aacaagcaga
     8821 agatttagac ggtctaggct acttgcaagg aaaagaagtg gacttcgtaa atacaaaagc
     8881 atttgaagga acattgcttg ctcatacaga cggtgacgtg cctaacttgc tagtcaaaat
     8941 tccagaaatg gatgcttata cattgggtta tacaatgtac ttctttgaaa ttgctgtagg
     9001 tatttcaggt tacttgaatg gaatcaaccc attcgaccaa ccaggtgtag aagcttacaa
     9061 gaaaaacatg ttcgcacttc taggcaaacc gggctttgaa gatcttgcta aagaattgaa
     9121 cgaacgcctg taagataagg gtttttcgct agatttaaat aatcagtgat agaaacatgt
     9181 tcgctttaaa atgaaaactg gtacccattt aggtactcaa acatactgca tcgtttattc
     9241 agaataaggg gctgtgacaa gacgtttgtc acagtccctt gtttgtatag aaatcgattg
     9301 attgccttgc tctttagttt gttttaggac tttaagaaca gtgaggctga cttcgaaggt
     9361 taattctgtt tttttgtttt ctagaatcag taagcaaaaa agcactcact tcgagtgctt
     9421 ttttgcgata tattcttagc ttaagatagg cctaattttt cttttacaga tgagggtacc
     9481 ttatcgctgg taattacttt tttctctatg atccgctttt cgcttgcttt caactgcaag
     9541 atcgttcctt cggtgtaagc agaaccattg gcagagtcac cagaagtaat gacatcgaaa
     9601 tctaactgtc ttttttcacc gttttcagca taagcatcta cgttgtattt aaaacctctt
     9661 ccaattactt ccccattatc tgctttgata tcagtttctg caggcattgg agctttgact
     9721 accgcataat aatctttacc tacataagta gactgatagt agtcccaacc tttccatcct
     9781 ataaaagcaa tcaatatgat aacgatcaac cctaaaattt ttttcatgaa agatctcctt
     9841 ttttattttt attatattaa atattataca gataataacg gaatattatc atcgaaacag
     9901 ctaacaaaat tgtcataaaa aacgagatgg ttgatattag aacttgtgtt tttgtttagg
     9961 tctagagata aagagagaca atttccaaat atatgtaatg aatacaaaaa gacgtttgtc
    10021 gcggattacc gttacaaacg tcttttattg attttccgag atctcatcag tcgccatttg
    10081 gaatggttca ctccttatct agattcgttt aatgctcgga ctgcttgttc gattgtaata
    10141 ccaaacgcaa cgttattttc gttttgtgca tcaatcgcca taaaacggtt agaagttgta
    10201 tcaaaagcta cgcggtattg catatttgtt tttgtaaacg tcataaaatt catctccttt
    10261 gtaatgtaat ttagttcttg aaagtttcct ttaaactttc gaacttatac caagtttagc
    10321 atcgtacgtt ttttgatgca attaaaaagc atttatcatt ttccttattt attttttctt
    10381 aaatggttta gctattttct tttaatccga taagtagccc aatcatcaag gttacaaata
    10441 ctattgcttg aatataaatc atatgctcac ctctttataa ataacttagc aatataaaga
    10501 aaaaaacgca cgaacaacat aggaatataa acaaacgttt ttgtcacaac gtctaagatc
    10561 gtgttaaaaa atagttatct aaaaagcaga aagatactca aaataaaaaa taaaaaaatg
    10621 aataaaaacg ttttcaaata caaggtaatt tgttattata aaataaagaa aaaacaaaat
    10681 aaaaataaag aaaagaggga atgaaatggt aaaagcagaa gtaggaagat cttatatatg
    10741 caaaccgatc ggagtatctc atgaactaat cggctgtatt gaacgtacat atatcaacac
    10801 ggctgtcgtg tatgttgaaa gttatgacca acgagactat caacttatca atgaaagcaa
    10861 gtatcgcatg ctcgtaaaat tctgtgatat tagcgcacct actgaattgg tttcttaaac
    10921 agtatttttg agaattcgca agaactccct tataatataa ataaagacag aagggagcga
    10981 gaagatatgc caaaaagaaa aagttcaggg aaaatgggaa ttattcttgc agtgatctct
    11041 gcaattcctg ctattttagc tattgttcgt gcgatgaagg aatcaagaga aagttaagca
    11101 cacattatga gcgtcaaaca aaaaacgcat atacctgcgg tggcaggtta tgcgtttttt
    11161 gttgtataaa gacttttttc attaacgatt tttgattagc cgactcccaa aacaaaattt
    11221 agcagtttat ctgaataagt gatttcttca tctgtttgtt tcacactttc atttagtagt
    11281 cccatctcat cggttatgat accatccaca ccataaaaca tcatgcgact catggcatct
    11341 gaatcgttga ttgtccatac atatacttct tttccgtctt ggtgggcaga attaatgaag
    11401 ttacggttga tcgtactata ttccattgtc agaaaatcag caggcgtcac agggggccca
    11461 acaatattga aaggtaagat gtagcccaca taaaagtttg gttctgcttc ttttaaatct
    11521 gaaacggttt ggtacgtcaa actttgcaaa atatgatggt tctctaagat aactttgcgg
    11581 tatttatgga caaatcgttc gacaaggtca tccgaatctt ttttagaggt cttgatctca
    11641 ataagtaatt tttggtttag cttatgtgct ttttccaaat aagaatcaaa agaacaaaca
    11701 ggggcttgca ctccgttttc agtaactgta agatttgtca attctttcaa tgtaagatcc
    11761 tgtggtgttc catttacccc tgttaagtga cgaagatttg catcatgcat cactacgaaa
    11821 tgaccatctt ttgtttcctg aacatccatc tctacataat caggatgata gtctctgctt
    11881 gttttttcaa gtgcttctaa agtattttgc gccccatttt gggtactcac acctctgtga
    11941 gagacagtca ctggttttgt ctgaacagct gaagtcagat aatccatatt gtaaagacag
    12001 acgccaatac cgaaaaaaac tgcaaaaaga gtcaaaccag tgtttttcag cgcagagaaa
    12061 cgcaagttgg gggcttgtgg gataaaccac ttagggatct caggcaagaa gccttcgtca
    12121 tccatgaaat caacaatgat ataaaaaatc cctacggtag acatcacgat attgaataat
    12181 aaaatccctt gtaaaagcgt catagcaaac acagcgctga tcaatgcata gtctgggaaa
    12241 aattgttcaa ccatactttg tgccaaaata acaacgatat atcctaaaga agaaaggagt
    12301 aaaatcgtac cgccaatcac aataaattgg cccgtgatgg ccaaaagacg cgatttggtc
    12361 aatgaccagc tttcccgaat ggctgctcga aaagaacgat ctcgtagaat catttccggt
    12421 aaagcaaaaa tcagtcgaat cccaatatag ccaaggaaaa tataaacgag taaaaaggaa
    12481 ctgacgatga tccaacgatt agcaaaaata aaatccataa tgaaagcggg gatttttatt
    12541 tttgacagta gatcagaatt aaaacttaat ccgctgatcg gtagaatcaa taaaaaataa
    12601 gcaaggaaaa acaaaaaagt aatgggtctc actttcttca gctgaagtat cgtcaagtgt
    12661 aagagttgct tcaaagagat tggttctttc tttttaataa aaaaaacact catcaaaaga
    12721 aaagtaaatt cgaaataaac taacagtaaa atcgtcatca aaactacgat taacgagagt
    12781 agaaccccag gatgttgaga aaaaattgaa ggaatattgt cataagacaa atagtcaatt
    12841 gcccctcggt gtaaaataaa acgggtcgta ctgctcaata agggtaaaca aataaataaa
    12901 ataaagccat gcatcagcaa gacatcacgg aaataagctt gtgttccttt taaaaagtcc
    12961 caagcattac ctaagctacg tttcaaataa ttcatcctat cacctgctct tcacattgta
    13021 gcgcaaatct aatgaaaaag gaaagggtgc agtgtgtttt gcagaccctt tcccagattt
    13081 aa
//
LOCUS       DAZULG010000077        12913 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000077 DAZULG010000000
VERSION     DAZULG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.079"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG9CY_001990"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG9CY_001990"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS4938492.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACG9CY_001991"
     CDS             complement(1034..1954)
                     /locus_tag="ACG9CY_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4938493.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACG9CY_001992"
     CDS             2067..2819
                     /locus_tag="ACG9CY_001992"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS4938494.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3683..4216
                     /locus_tag="ACG9CY_001993"
     CDS             3683..4216
                     /locus_tag="ACG9CY_001993"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS4938495.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4706..5230)
                     /locus_tag="ACG9CY_001994"
     CDS             complement(4706..5230)
                     /locus_tag="ACG9CY_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS4938496.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5223..5696)
                     /gene="tsaE"
                     /locus_tag="ACG9CY_001995"
     CDS             complement(5223..5696)
                     /gene="tsaE"
                     /locus_tag="ACG9CY_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS4938497.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5819..6799)
                     /gene="pta"
                     /locus_tag="ACG9CY_001996"
     CDS             complement(5819..6799)
                     /gene="pta"
                     /locus_tag="ACG9CY_001996"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS4938498.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6952..7632)
                     /locus_tag="ACG9CY_001997"
     CDS             complement(6952..7632)
                     /locus_tag="ACG9CY_001997"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4938499.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7758..8627
                     /locus_tag="ACG9CY_001998"
     CDS             7758..8627
                     /locus_tag="ACG9CY_001998"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4938500.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8653..9168
                     /locus_tag="ACG9CY_001999"
     CDS             8653..9168
                     /locus_tag="ACG9CY_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS4938501.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9215..9778)
                     /locus_tag="ACG9CY_002000"
     CDS             complement(9215..9778)
                     /locus_tag="ACG9CY_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4938502.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9833..10012)
                     /locus_tag="ACG9CY_002001"
     CDS             complement(9833..10012)
                     /locus_tag="ACG9CY_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938503.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10089..10598)
                     /locus_tag="ACG9CY_002002"
     CDS             complement(10089..10598)
                     /locus_tag="ACG9CY_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938504.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10694..11074)
                     /locus_tag="ACG9CY_002003"
     CDS             complement(10694..11074)
                     /locus_tag="ACG9CY_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4938505.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11404..12153
                     /gene="pnuC"
                     /locus_tag="ACG9CY_002004"
     CDS             11404..12153
                     /gene="pnuC"
                     /locus_tag="ACG9CY_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS4938506.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12474..>12913)
                     /locus_tag="ACG9CY_002005"
     CDS             complement(12474..>12913)
                     /locus_tag="ACG9CY_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4938507.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         3733 a         2612 c         2321 g         4247 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta
     3061 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3121 ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     3181 ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa
     3241 tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac
     3301 cgtttattga gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca
     3361 aaatagttcc tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca
     3421 ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg
     3481 aaaatggttc ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca
     3541 tctaactcca aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat
     3601 aaaaaattct atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat
     3661 ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca
     3721 tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga
     3781 tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca
     3841 cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat
     3901 ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa
     3961 ggtcttagcg ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg
     4021 tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt
     4081 gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc
     4141 atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact
     4201 tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt
     4261 tcactccgag tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt
     4321 gactggtgac cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct
     4381 gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg
     4441 ttggacttat taccgcagat gcaagctttt ggacaccgtt taccaagagg ttgtgaaatc
     4501 agcgttttga cctgagtatt agagaaacaa acggcaaaat agttcctcat attttgccgt
     4561 tttttgagct aataccgaag gtcagctgat tgaacaccgt ttattcaaaa aaaatggact
     4621 gcgacacgac ttttgtcaca gtcccttcgt gtgtttttct gccttctcaa aaggatgaca
     4681 gtttctcact cttttcctat ttcttctatt caatcaaata actcatttta tatacatcta
     4741 aaaattcacc tagatctgtt cttgcccctc taggcatgac cgcttcgatt tggaagccca
     4801 tttttcggta aaggtgtacg gctctttcat tacgaacttg aacgtcaagc tccaatcgaa
     4861 aaagtacacc catttccttc gcccaactga tgatttcttc tagcataaga gtgcctagtc
     4921 ccattcccca atattcctgc agaatactga taccgacttc tcccacgtga gacaaacgaa
     4981 attgactgtc tgccttgaca gatgctgttc caataatcgt cccttcatag attgcaagca
     5041 gcaataaatt attattgctt tcgtatatat aatccaactc ttcttctaag gtttctggtg
     5101 gtaacaggag ttctgcttca tccaaaacta aaaattctgt ctccttatta acttgtccca
     5161 tcatcaaaag aatgtctttt gcatctcctg gcactgcttc ccgcaagatg atcccaactt
     5221 cttcactcat gattttcagc ccatttttct agaattcgct gtttgaaggt ttcactttgc
     5281 tccccgtatg agattagttt cacataccgc ttttctaaat ctgtatcact tttttctaaa
     5341 atcagttcca aataatcctc cggcagtgct tcttctaata gatttcccca ttcaacaacg
     5401 gaaagcccat caccttcaaa atattcatct aaccctaaat ctgctccgct agctgctacc
     5461 cgataaatat ccatatgata caacggaatc cgaccttgac tatattctct aataatcgtg
     5521 tatgtcggac ttttgatcat ttgcgttatc cctaatcctt gggcaattcc tttcgtcaac
     5581 gttgttttac ccgcacctag atcaccagtc aatatcagat tgtcacctgg cattgcggaa
     5641 tgaccaattg cctctgcgaa ttgttctgtc attgttaatc ctgatagttc gatcattttt
     5701 catcttctcc cgctcttctt tctttttgcc agtatatcgc gtaatgaagt ttttttaaag
     5761 aaaaaatgaa ctagtcaaga acagcagctg ctgtttttgg ctagttcgcg tgtatttgtt
     5821 aatcaatcaa tgtttgtgct gctgtgataa tcgataattt atatacatct tcttcatttg
     5881 ctccacgaga caaatcagaa accggtttgt tcaagccttg tagaattggt ccgattgctt
     5941 caaagttacc gaaacgctga gcaattttat agccgatatt tcctgactgc aattctggaa
     6001 atacaaagac tgttgcttga ccagctactt ctgaatctgg cgctttcagt tgtgcaactg
     6061 aaggaacata agaagcatca aattgcaatt cgccgtcgat catgtattgt ggcgctagtt
     6121 ttttagcgat tttagttgct tcaactactt tgtctacttc tggcgatttc gctgagcctt
     6181 ttgtcgagaa gctcatcatt gctacttttg gttcgatatc gaacaattct gctgtttttg
     6241 cagaatctac ggcgatttca gccaattctt gcgcagttgg attcacgttg atcgcacaat
     6301 ccgaaaagac atatttttct tgatctcttc cacgtaccat gatcatcgct ccgcttgtcc
     6361 gactgatgcc aggctttgtt ttgatgatct gaagtgctgg gcgaacagtg tcgccagtcg
     6421 aatggatcgc tccagagacc attccgtctg caatccccat atacgtaagc atcgtaccaa
     6481 agtaattcac atcttttaaa atcttttctg cttgttcttt ggatgccttt ccatttctac
     6541 gttcaacaaa agtatccacc atttcatccc acttatcgta attatcaggg ctgatgatcg
     6601 taaagctaga tgttttgatt cctctggcat gcgctgcatt tcttatttct tcttcgttcc
     6661 ctactaaaat gggttccaca agttcttctg ctttcaaccg ggcagctgcg cctaaaatac
     6721 gagcatcagt agcttccggg aaaacgattt tgatcccacg gcgaacaatt ttaaatctta
     6781 aactatcaaa caattccaca agatgacctc cagacgtttt tatatgtatt atcatacact
     6841 attccagaaa aaaagaacag tttattttat ctgtatcagt caaaaatgct ggaaaaatta
     6901 agtttttcac atacaaagcg aactgcacta taacaggaaa gcgctgtttt attacactgt
     6961 ttcaggcagt tgccaatcaa tcggttcttc tcctaacgca aggagcgcat cattcgtttt
     7021 cgagaagggt ttggaaccaa aaaagccacg acttgcagat aacggactgg gatgtggaga
     7081 tgttatgatg atatggcgac tcttatcgat cattttgatt ttttcttgtg ccggttttcc
     7141 ccacaagata aacacgattg gtttttcccg ttcatttaat ttttcaatga tcgtgtctgt
     7201 taatctttcc caacctttcc cgcgatgaga atacgcttgc ccttcccgaa cggtaagaac
     7261 ggtgttgagt aaaaggactc cctgtttagc ccatgacact agatttccat gttgaacagg
     7321 tgcgattcca agatcatcat aaagctcttt atagatattt cttaacgatg gcgggatctt
     7381 tacgccaggt tgcacagaaa aagataagcc atgagcttga ttttctccat gataaggatc
     7441 ttgtcctaaa atcaccactt taacttcttc ataaggagtc aattccaatg cttcatagat
     7501 atgatacata tctggatgga tcttttggga cgcatattct ttttttaaaa attctcttag
     7561 atgcagataa taatcttttg aaaattctga ggacaaaact tcttgccaac tgttatgaat
     7621 gatttctttc aaaaactcca cctcctaata aaaagaataa ctcacatttt cttcaatcac
     7681 aagggacaat cttcaccctc agcacttgat taatatggta aactaggaac aagaagcaga
     7741 aaggaacgat aatcattatg atcaaattaa ttgcatcaga tatggacggc acattattag
     7801 atgcccatat gagtgtttct cctgaaaatg ccgaggcgat acgttttgca aatgatgccg
     7861 gtgttgagtt catggtcgct actggtcgaa actatcaaga agctcgcgca gctttagatg
     7921 aggtaggaat cgattgtgcg atgatcactt tgaacggggc acaggtattt gataaagaag
     7981 gaaattcatt gtttaccgtg tcgattccaa ataatcaagc aatctctgtt ttagatattc
     8041 tagatgctaa cggtatttac tatgaagtag caaccaacga tggtctttat tctgaaaatc
     8101 aagcgaaacg aattgaaagt tttgcttcca tggtggctac ccatttacct caccttacgt
     8161 ataaaatggc gattgccatg gcctcagcta agctggagtt attgcacatc acatacgtgg
     8221 acagtattcg tgctattttg gaagaaaaga atctggaagt tttaaaaatt atttgttttc
     8281 atacagaagg gccagctgta ttaggtccag ttggaaagca gatttctctt ttaggcgatc
     8341 tagccgttac ttcctctggt caaaacaata tcgaagtgaa ccataaaaac gctcaaaaag
     8401 gaattgctgt tgcccatgtc gctcacgagc gtgggatttc tttagaagaa gtcatgacga
     8461 ttggcgataa cttcaatgat gtcagcatgc tgcaagtagc aggagtcagt ttcgcaatgg
     8521 gaaatgcaga aatcgaagta aaagattatg ccaaatatac aacggacaca aaccttgaat
     8581 ctggcgtagg aaaagccata ttgcgcgcga ttgcagaaga cttgtagttt tatctagaaa
     8641 agagggatat agatgtcaga atttttcatt caggaaaagc agcttggacg tgcaactcgg
     8701 acaattgtca aaaacgaaaa aggacattcc ctgtttttga tggttggtcg ctggggaaca
     8761 aagggcgatg cattgtctct ttatgccatg aatggcgatc tcgttgctca catcaagcag
     8821 ataagcctga cattcggtac gcgttttgag ctgtataaag agttcgaaaa ggtgggaacc
     8881 atgcagaaaa tattcaattg gcccggtgac ttttattata ttcgtagact tcactggaca
     8941 gtccaaggtg atatctataa tcatcaatat caaatccatc atttcaatca gcaaatcatg
     9001 aaaatggata aagcaacact tttttcagga gattactatg tattggatgt ctcaaatgat
     9061 gaagatgctc cagtttgtat ttgtattgct gcggttttag attactggct ctaccgtaaa
     9121 gacaaaagtc gcgataagcc tgtttcttac cgacttaact taagttaaaa agatgtacct
     9181 gaaatgcgga aatacgcatt tcaggtacat ctttttagat attcttttgc atatgatcat
     9241 aaagatgacc actgatcatc cgctgaccga caactttgaa gtcatggcgt tcgtataatt
     9301 ttttagcctt tggatttttt tcatccacac ttagaccgat gacggagcga ttagctttct
     9361 tcgccaattt aggtaatgct tctaataatc gcgaaccgat gccttgtcct ctaaaatctt
     9421 ctgatacact gatagagtcc aaataccatt cgttgggaaa cgtttcttta tcaatgaaca
     9481 aacgtgtaga cgcatctagc tgatatttct ctaagatagg taccagtggc tgatcgatga
     9541 ttgattcttc ttcatctgta taaccgtaag caactcctgc aactttaccg tctatttctt
     9601 ctacgatacc tcgtgaataa ctgtaacgat aatttggatc agtgattgct tcttctaaaa
     9661 tctctaaagt cttttgttcc cctgtctgct tcaaaaatgg cagttccata tcttttagaa
     9721 tcactaagat caatggggcg atttctggag catcttcttt tctcgcaaaa cgaatcatag
     9781 tttcctctcc ttttctccat tctaacatgc agaaaaagaa atcgcaaaat cgttatttta
     9841 aaaaatcttc aatcttttca gtaatgaaat aaccatgagt aacgattctt gccgcttcag
     9901 ttcggcaata agggcagacg actggcattt ctttatcatt atgttcgact acattattga
     9961 cttcttcgta tgcaaatttt tttccgcaat tagggcattt cattttttcc atgttatctc
    10021 aacttccttt catctttact ctcttagctt actcttatat tgtaaaaaag agaagcaata
    10081 tccttcttct atgcagatat ccatacctga cggtcctgtt ctccatctat taatcggact
    10141 ttataataag gacgttcatc tttgactaga agtgtccact ctttcacttt tgcttttttc
    10201 ccgtagtagc tgactgcttc cttcatcgct gcttgcggtt gtttgaactc ttccgttttt
    10261 tctatatcaa aaagatgttc tgcggccttt tccgtgtcat ttcgtacggt tgttttccca
    10321 gtggatggat tgactgctat gacttgtcct tcacaaaaaa actggtattc atatgttgca
    10381 ttcgtaggat tctccaaatc tttttcaact gcaaaatgaa tagcagatat gttatgttct
    10441 tttgtttcgt tttttacgat tgctgctgct tttttcaatg agatccgata ttcctggtca
    10501 ctaatatcat ccatgtgaaa agcggcattg agcagtcccg tcataccggt aatccctatt
    10561 acgatgaggg cagcaacacc aaacatcatt tttttcattc ttcattctcc tgtttctttt
    10621 ttttaaagaa tatcaagaaa tgaacgaatg aaaaatcgaa tgaaacgaaa aaaaagtgac
    10681 aattttgtta tctttattcg tgcagttcta atggcaatcc gtctggatca aagaaaaacg
    10741 tcattttttt tcctgtaaag gtatcttctc taatcggttc tgattcgatc cctaaggact
    10801 gcaattctgc aattgtttct tcaatatcgt ctactttgaa agcaagatga cgcaagccac
    10861 aagcttctgg atagtttggt cgttgtggcg cattagcaat tccaaaaatc tccaattcag
    10921 cacttcctag tttcagatct aatttatagt catctcgttc tttgcggtaa ttttctctga
    10981 ttacttcaaa tcctaattta ttaacataaa aatcacgtga tttctgataa tctgaaacaa
    11041 tgatggctat atgatggatc gttgtaaggt tcatatgagc actcctattc ttcgtttaag
    11101 ctcggatcta tattaatctc cagttccatt gtaaatgtgt cagattgaaa aaaatagctg
    11161 tttttccttt ttataagaaa aaaggcaaaa aaaacagaaa gtaggtcctc caagacactc
    11221 ttcctgttcc caatataata aatgaaagta agaaattata ttttcaagaa aacaaacaga
    11281 aaattatcat aacacaaatt atttataatt gaaagactag ttttttttca agaaaagcat
    11341 acaatagatt gtgttagtaa atacagaaca cactacattt agtgtcttaa ggagtcctca
    11401 tacatgaagt catattttca gtttttagct catcagctaa aaggctggcc gcaacaaaat
    11461 tattatcttt tctttttcag tcttggctgc caggtcatga ctcttgtcaa tagtcccatc
    11521 acttggctgt ccgttattac atttatcggt accacgcttg gtgtattgtg tattttatcc
    11581 atcaatgcgg caaaatcagt caacggtatt ttaggtattt tgtctgcgat ttgttttatt
    11641 atcgttgggt tttcagcaaa gaactattta agtatcggtg aacagattgc ttatatgatc
    11701 acattggata tccccgtatt gttaagtaaa gaatggaatc ataatatggc ttcaaaaatc
    11761 cgaaaattca ctggaaaaag ctggtttatc gcaatcatca gtactttcct tgtctatctt
    11821 gtttcaggat tcctgattgg tcgttataca gatgatcctc ggccgtgggt agatgccatt
    11881 gctttttcta ttagcttgac tgctggtatc atcagtttct tgcgttacaa caaccaatat
    11941 tactggtggt tagcttcagg gcttgctcaa atggtccttt ggtatatttc atttacgcat
    12001 ggatctgcaa gtttggcgat gttcgtgaac agctctgttt acttgctgaa tgatgtcctt
    12061 gcatttaccg tttcaccttg gtataaccaa aaagaacgtg aacgtatgca aaaaatggaa
    12121 gaagaatact acaacgcaca acaacacgca taaaaaaact gagcgtggga caaaagtaaa
    12181 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaatcagaag caactccttc
    12241 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
    12301 cttggagtta aacgcttctg tcccagcctc tatttcctac gattagtcaa gtaaaaggtg
    12361 caagaaaaat agctcgtaaa cgaaagcgac aaaatcaaag cacaccaaat aaagccccgt
    12421 tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt tagttaatgg
    12481 tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat agcgtgcata
    12541 catttcagaa gcttgttcat acaagcaact gtggcaacct tatctttctt agggataggt
    12601 tgctttttta atttgtagta ataatcgaca atatgattag gagctgcgcg ttgctggcga
    12661 atcatatttc gaatggtgaa gaaaataatt ttccgtgctt tgggattacc acgcttattg
    12721 atgcggtctt ggccagtata cttacctgaa tgataccgtc ggatgtcgat tccgacaaag
    12781 gcattcaatt gtttatgggt tttaaatcgt gtgatatctc ctaattcacc gagcaataaa
    12841 gcggctgtga gtgcgccaat cccagggaac gtttggtaca acaaaaagca aggagagcct
    12901 tcggcttttt tga
//
LOCUS       DAZULG010000078        12867 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000078 DAZULG010000000
VERSION     DAZULG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.080"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..1167)
                     /locus_tag="ACG9CY_002006"
     CDS             complement(19..1167)
                     /locus_tag="ACG9CY_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4938508.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(1289..1885)
                     /locus_tag="ACG9CY_002007"
     CDS             complement(1289..1885)
                     /locus_tag="ACG9CY_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4938509.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2085..4727)
                     /gene="alaS"
                     /locus_tag="ACG9CY_002008"
     CDS             complement(2085..4727)
                     /gene="alaS"
                     /locus_tag="ACG9CY_002008"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS4938510.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5115..5546
                     /locus_tag="ACG9CY_002009"
     CDS             5115..5546
                     /locus_tag="ACG9CY_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4938511.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5591..6940)
                     /locus_tag="ACG9CY_002010"
     CDS             complement(5591..6940)
                     /locus_tag="ACG9CY_002010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4938512.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7010..8173)
                     /locus_tag="ACG9CY_002011"
     CDS             complement(7010..8173)
                     /locus_tag="ACG9CY_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4938513.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8302..8625
                     /locus_tag="ACG9CY_002012"
     CDS             8302..8625
                     /locus_tag="ACG9CY_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938514.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8808..10157)
                     /locus_tag="ACG9CY_002013"
     CDS             complement(8808..10157)
                     /locus_tag="ACG9CY_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4938515.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10226..11179)
                     /locus_tag="ACG9CY_002014"
     CDS             complement(10226..11179)
                     /locus_tag="ACG9CY_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS4938516.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11281..12471)
                     /locus_tag="ACG9CY_002015"
     CDS             complement(11281..12471)
                     /locus_tag="ACG9CY_002015"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS4938517.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(12671..>12867)
                     /locus_tag="ACG9CY_002016"
     CDS             complement(12671..>12867)
                     /locus_tag="ACG9CY_002016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938518.1"
                     /translation="IQQVKLLRNIQRVKLKIADKRRWKFYVFLLVYAINKLNIQNTID
                     FNERASINGCVVFICPGRVK"
BASE COUNT         3695 a         2707 c         2219 g         4246 t
ORIGIN      
        1 ctttttatag tttctcattt aatataaaag ataattgtga tcaaacgtaa tttccaattg
       61 gtaatctccg attttttctt ctatttgact acgtaagatt ctttctagtt ctgtatcaac
      121 aaactggaat ttctcaggga ccactacatc aaattgcagc gtttttcctt gatgaattac
      181 tctaaaatca tgaatattaa gccctgtacc tgtttcaaga agaatttgct ttaactgccg
      241 caagatatta tagtattttt cgttatcaac aggcattgga tccaagtgac agacaagttc
      301 cacatccaaa gactttttaa agtctttttc tatcagatca ataatcttat gagccacatt
      361 caaattcatt gattcatcta cttcgatgtg gactgatgca aagcgtttat tgggtccata
      421 gttatgcacc aataaatcat gatagccaag tatatcatcg caagcattta aatgctcttc
      481 catttcttca atctctgctg gcgttgggcg acttcctaat agttcgtata caaaatttcg
      541 aagcatcgtc aatccactat aaaggatata taatgctaaa gcaaaaccta tatatccatc
      601 aatacgccat ccagttaccc attcgattgc tgctgaaagc aatacagcca gtgtagtaaa
      661 gacatcatta taactatctg ttgctgtagc tctcaatgtt tcagaatcaa tggattgcgc
      721 aataacctga tacattttgc cttgtgcaaa tttcaaaaag atggaaatga ttaggacaaa
      781 aaagacgatg ggtgttaaag agacattttc tggatggatg attttttcag cagaagactc
      841 taaaaattga aatcctacat aagtaatgat gatcgaaaca aataacccac tgatatattc
      901 gaagcgttcg tgtccataag gatgttcctg atctgctggc tttgcagcaa ttttaaatcc
      961 gatcaaagtc aatatggatg aagcagtatc tgatagactg ttcactgcat cagccataat
     1021 ggagacactt cctgaaaaaa gcccgatcat tagcttagag acaaacaaga tcagattcgt
     1081 aaccaatcct aagatacccg ctagtactcc tttttttgtc cgctgttgcg gtatttgttt
     1141 ctttttttct tcttgtttct tagccaagtt ttttacccct tctagtttgc ttgttgctca
     1201 ggtaaaaaga tggagaggac gattgttttc ctctcctctc ttcctttttc tatctatcgt
     1261 gcgtcctatt actattaaaa gatggtcatt attctggttt caacggtgtt gctgcaaata
     1321 acgcttttgc tagtaatggt gttatgattc ccgcacctat tgcttctggt atcccattga
     1381 ttgctgcaac ccctgctaag gttttaaata aaagagcaga gtctacacca taggcatctg
     1441 ccgttgcacc tgtataaaat aatcccatga atcctaacac taagactgta ttgaccaatg
     1501 aacccagtac ccctgcaaag gctgagctga tcgttattga tttatttttt tgataaatcc
     1561 agtggaaaac caagcctgct actaagccaa caagtactct gggaactacc gagataatcg
     1621 gattcgtgaa cactaatgta tcaaatggcg ttgttggcat ggcataggca cgaacaattg
     1681 tagcgattcc ccaaaacaag ccaatcacca tgccatcttt tgggcctaat actactgcag
     1741 caataattac tgtgatatgg ataattgtta aactaatgaa tcctaatgga ataaacccca
     1801 gccaaggaac catcgcttgt acgatgatga ttgctaacaa gatcgcacgt aagactagtc
     1861 gaaaggtctt atttctacta ttcatttttt cacccctttt tatttatact aatcgtaaaa
     1921 atttctgtca ctttcgtcaa ctaaagacca tccgaatccg accatttgat tagatgcgcc
     1981 atccaattga aaaaagtatg agggcaagac ttgtttgtct tcgcccccat aatcatgttt
     2041 cgttcaatgt ttgaactttt gtttttctgt caaacgtaaa cgccctattg ttgcgcaacc
     2101 cattctttcg cagctttaag tgcatctgga ataccagcag gatttttccc gccagcttgt
     2161 gccatgtctg gacgtccacc accgccaccg cctactttcg gtgcgattgc tttaatcaag
     2221 tcgccagctt ttagtccagc ttcatttgat tctttcgtca ttgccgctag cagactgact
     2281 tttccttctt gttcgtttgc taaaacaaga atatctgaaa tatttttttg tttccattgg
     2341 tcagctaatt gacgcagctg attcatatct ttcactttta cttgtgctgc aatgattgtt
     2401 tttccattga tcgtttctgg atctttgaat acatcaccag cttgttgatt cgctaacttg
     2461 ctagctaatt gttcattttc cttttgtagc tctcttactt gctgttgcaa ttgttccgct
     2521 tttgaaacaa cttctttcaa ttgaggtgat ttaacaattg ttgctacttg ttttagttgt
     2581 ttttcttctt cttcaagtag ttcgtacgct tctttacttg ttacagcctc gattcgtcgc
     2641 acgcctgcac caattcctga ttcagaaacg attttgaaga ttccgatgtt ttctgtattt
     2701 gctacatgtg tgcctccgca cagttcgatt gaccagccgc caatatttac tactcgaacg
     2761 tcatgtccgt atttttcacc aaacaaagcc attgcaccca tgtttttggc tgtatcgata
     2821 tctgtttcaa ttgtttcaac tgggatagct tcccagattt tttcgttgac gattctttcc
     2881 atttcagcta attcatctga agtgatttga ccaaagtggg taaagtcgaa tcgtaaatgt
     2941 ccaggagcga ctaaagaacc agcttgattt gcatgctcgc caagtacttc tttcaatgcc
     3001 ttgtgcagta gatgggttgc tgtatggttt ttgatgatcc gattgcgcat cgattgatcg
     3061 atcaatagat gataggtttg tccttcactc attggtgctt ttacttctac ggtgtgcata
     3121 aattgaccat taggcgcttt ttgaacatct gtaacaactg ctacgatttc gcctacttca
     3181 ttcatgatcc atccttgatc cgctacttgc ccacccattt ctgcatagaa aggtgtttga
     3241 tcaaagatca tttgagcttc accttctgcg acttcattca aaagagactc acctttaacg
     3301 atgaccaata attgactatc tgattcttgt tctgtgtatc caatgaattt actttctact
     3361 ttgatatctg tcaataaagc agattgtacc cccattgatt tttcagtact gcgggcagag
     3421 cgagcacgtt cgcgttgtgc atccatttct ttttcaaaac cagcatgatc aactttcagt
     3481 ccttcatctt ctgccacctc ttccgttaat tctaccggga agccatatgt gtcgtatagt
     3541 ttgaagatgt cttttcctgc tagagtcgtc ccgttttcgt tttttacttc ttggatcact
     3601 tgatttaaga tttccaaacc ttcattgatt gtttcattga aacgttcttc ttctgtacgg
     3661 ataacttttt caataaattc tttttgttga agtacttcag gataataact taccatgatt
     3721 tccccaacaa cagggactaa acgatataag aatgcttgtt caatacctaa ttttttccca
     3781 tgcatgacag cacgacgaag caaacggcgt aagacatacc ctctcccttc attcgatggc
     3841 aatgcaccgt cgccaatagc aaatgaaagg gcgcgaatat gatctgcgat tactttgtaa
     3901 gaaatatctg ttactgggtt ttcaccatat tttacttgtt tgcttagctt ttctacggcg
     3961 tggataatag gcataaacaa gtctgtctca aagttcgttg acgcatcttg aacgattgag
     4021 accatgcgtt ccaatcccat ccccgtatca atgtttttat gaggaagtgg ttcaaatgtg
     4081 ttatcaggct tatggttgaa ttctgaaaat actaagttcc agatttctaa ataacgttca
     4141 ttttctcctc ctggatagtt ttcaggatcg tcttctgcta aatcgttata agcttctcca
     4201 cggtcataga aaatttccgt atccggacca catgggcctg caccgatatc ccagaagttg
     4261 tcttctacat caataatgtg atctagtggc aggccaactt cttcatgcca gatgcgtttt
     4321 gcttctgtat cttttggata aactgttaca taaagttttt ctggatcgaa tgccatccat
     4381 tttggatcag tcaaaaattc ccatgcccag tggatggctt catttttgaa atagtcacca
     4441 atcgaaaaat tacccagcat ctcaaacatc gtatggtgcc gtgctgtttt accgacattt
     4501 tcgatatcat ttgttcgaat agatttttgg gcatttgtga ttcttggatt ttctggcaca
     4561 actgaaccat caaaatattt tttcaatgtt gcaacaccag agttgatcca caataaggtt
     4621 ggatcgttca cggggatcaa agacgcacta ggttcaatcg tatgtccttt agattggaag
     4681 aaatcaagaa acatttgtcg aacttgtgca ctacttaatt gtttcatgtc attacctgct
     4741 ttctataaat taaatacaaa aaaacaccat cacagccaag gacgaaaact cgcggtacca
     4801 ccttgattgc aatgatgtgt aatcattacc tcttaaactc gataacgctg agctgcgtcg
     4861 gtatttctac caaaatagaa aaagggagca atttgattag tgactattct tttttcagcc
     4921 aagaaagaat tttctgtgag tcttaaaaaa caaatcatat ccttttcgta cttaattagt
     4981 atacgcattc tattgatcac tgtcaatatc tcaattttta ttttcatttc cttttggtca
     5041 tactccacga tttttggtac gataagaata aaggaatcaa tacatctctg ctattcatga
     5101 caaggaggaa tatgatgaaa aaaatagttg gagcggatga atggatacgc gcagctagct
     5161 gctttttgag atatcaagtt tttgtattag aacaagggat tcctgcagaa ctagaattcg
     5221 acggaaaaga tatcccgcaa caaacctatt atctatttct tgatcaaaaa agaccagtag
     5281 ctactttgcg ttatgaaaag aaaacaccta cttgcttcaa gccggaacga ttttgcgtag
     5341 caaaagaata ccgaggaaaa gggttaggcg gtcagttgct tgaagctgct gaaaaacggg
     5401 cggtttcaga aggttgtcag actagttatg tagtagctga aatgacggct gtagattttt
     5461 atgaaaaaca agggtatcat acttgcacag aaccttttat tgaagatgaa gtactttgta
     5521 tcggaatgaa aaaaagtcta gcttaaaaac aagagattgt gacacgcctt ttgtcacaac
     5581 ctcttgtttt ttatttatgt gaatttttct tatttttacg agcagtacgg ctttgctgtc
     5641 tgcgttcgat tttgcgttct tttttatttt tttctgcaat tgcccaatcg atttttttct
     5701 tataaccagg cttgatcttt ttctttttct tcttcacaag accaatcagc gtgggatcta
     5761 atacatcttt tgatttttct cgtttggcac gtctattacg atcgtatgtt ttgacgattt
     5821 ccccgttttt gatttctttt ggttcaaatg agacgcccaa tttttctatc gcatcaatgg
     5881 cttgctcatc atttggcgaa tataacgtaa tggccgttcc ttttaagcca ttacgtccag
     5941 tacgtccgac acgatggata aagaagtcta gatcttctgg aatttctgca ttaataacat
     6001 gagagacacc ttcgatatcg atccctctag cagctaaatc agtcgctaca acatattgat
     6061 actccagatt ctgtacttgt cgcatcactc gttttctctc tcttggggtg atgtctccat
     6121 gaatctttgc tactttcaag ccttggtttt tcaagtaatc ggcaatttca tccacatgct
     6181 gttttgtatt ggcaaatacg attgctaaat aaggatgacc aattgtcagc agttgataaa
     6241 tcatttgatt ctcatcttta ccttttgttg aaatcagcca attttcaatg tcttcagaaa
     6301 taactgcttt tggtttgatt tcttcaatga ttggattttc catgtatttt cgtaagaatg
     6361 gacgaagttt ttctggaata gtggcagaaa aaactaaaaa ctgcagcttt tctggtaatc
     6421 gacttgcaat ttggtctact tcatttaaaa aacccatatc caatgtcata tccgcttcat
     6481 cgacaacaaa tgcaaatgcg gtgtgaatct tcagcgcttg ttcattcatc atgtctaata
     6541 ttctaccagg cgtgcctact acaacctgtg gctgttggtg atgcagacga tttaattgac
     6601 gctgtttgtc tgtaccgccg acaaaattcg agacacggat ctccggtgta gaaaatttag
     6661 cgatttgtgt agctgcttca tagatctgtg cagctagttc cctacttgga gctgtaataa
     6721 caacttgtac ttcctctttt tctgcattga ttttttcaaa caaaggcaac aaaaacgtat
     6781 gtgtttttcc tgtaccagtt tgagattgcc caatgatatt ttttcctttt gcaattaaag
     6841 gaatcaaacg ttcctggacc tctgtgggct gtttgaagcc ctttgcggtt aacgcttcat
     6901 tgataaacgg tttgaagcgg aatatatcaa aattattcat tcgtgcacct cttctcttta
     6961 aattatcacg actttggcat tataacacag acaaagcgtt tttgctcagc taatttcgag
     7021 aatcatcatc ctttgtttgg ccaatcgtgc taacagtata tgtgtgacta gctttttcat
     7081 aaaacagaat ccgttttttt ctcaaaaatg atagcaagat atttaaaata aaaatacctt
     7141 gtagtaacaa gacaccgaga gaaatgaata ctgcactcat ctgcaacacc gtattcgttg
     7201 tcgaattcat gatttgctga agcaactgcc ccaaccagct taaaccataa aaaagaaagt
     7261 acagataccc gtagcggatc atcaatgctt ttaaagaaat tcgttcgcgg tcttcttcca
     7321 ccaaacggat acgcacaatt tttttgccaa gtgtccatcc attggttata aacggtacaa
     7381 tcataaagta tacccctact tctaataaaa gccaccagat atgagaagta actgcccctg
     7441 aatcgaacgc aattttaaag aaaatcgccg ctaccaaatt aataagagat aaaaatgtcc
     7501 agtcgatcaa ataagcgacc aagcgtcgag tcaatgagac agtctttcct ttttcataag
     7561 aaagttcatc cattcgtctt cttgtaggaa gcataaatgt aaataacggc gtaacgacaa
     7621 aaccaatgat tccacccaat gtatttgtca ctaaatcatt cacatcaaat aagcggtatg
     7681 gtcttgggta gataaaataa agtccactta gctgcgtcaa ttcaaaaaat agagaaaggc
     7741 agaaactagc aatcatcgtt ttcataaaag aaagccggaa ataatatctt aaataaatgc
     7801 caaacggtat gagtaataaa acattaaaag ctggttccaa aaatacactc tgcttcattg
     7861 ccggcaggta ggtactaggt tcagacaaga ccaataccgt gttgtctctg aaatgttgcc
     7921 atgaagcaaa taaatgcagc tccatctttg gccctgtcat ttgggcaacc gtctcctttg
     7981 ccggaagtgg taagatcacc aaaaaatacg cacacaataa ataaaaaacg aaagaataca
     8041 aaataactgc ccttgttact agaaatgttc catactttcg atattcataa ataaaaaaaa
     8101 cagatgagat cgctaaggct aaaaatggaa aaacaatcag cgcaaaacga atcggttcga
     8161 tataacttgc catatgactc ctccttgatt ctttgtatct ctcttatctt agcagtcctt
     8221 tgctgtttca agcaagtaac gaggctatta tttctcattt ctttcaaagt aaagtaagat
     8281 aaacacaaag ggggtgtcga gatgctgaat atgtactttg tttttggcgt acctattttt
     8341 ttactgttcc tttatgctac gatcgcttat gtaagaaaaa gaactacaat tcattattta
     8401 ggatttattc tcctgattat ttcgggtttc atgctagtct tcaatcttca gacttggcag
     8461 caagcacttc ttgaaatgga taaaatgact ccacatgctt tgtctaaagt gcttggctac
     8521 cctgtctatc tgatttggct tcccattttc atttctggtt gcttggtttt attaaatatt
     8581 tatcgcggcg tacgccgaat agttcagtta cgcaaatcaa agtaaaagac agaaatcggt
     8641 tgagcccgat ttctgtcttt tactttgcta tttcatcgta gacaaaacga catttacgtg
     8701 atcaaaatta acgagtactt ttgacatttc ttccacgtac tcctcattta aataaaatat
     8761 tttgtttggc agagaagtgc agttctaata aaattcgaat gttttagcta tctacgtttt
     8821 aaatagagat aaatcgggat accagcaagt gtcaatacta tccccatcga agctaaaaag
     8881 gtctgagtga taagcgtgct tgatacgata aaagtccctc caatgattgc tactagtgga
     8941 ataaacggat acattggaac tttgtatggt cgaaacaaat caggttcttt tttacgaaga
     9001 agaatgacac caacaaagac catcgtataa aaaatccaaa tgacaaagat cagcatatcc
     9061 gttagtgtat caaaaccacc gatcatcatc atgccaatag cgatgactag ctctaagata
     9121 cctgcggcta caggaacttt agttttatca tgaagtttag caaagagctt gctaaaaggc
     9181 aggttgtttt cttttgccat aacataaggg agacgcattc cagtaagggt atagccattg
     9241 atggttccat aaacagaaat caagatacca actgtgacca gtcttccacc gaagccgcca
     9301 aaaatcattt ttgcaacgtc acttgccgca ttgctgtttc ctgcgactcc gtcgatagat
     9361 gcggttctta agaacaccgc atttactaat aaataaacga tcatgattcc gatgattcct
     9421 aaagaaattg cttttggcaa atctttcgcc ggctttttca attctcccga aatatttcct
     9481 acgtgtatcc aaccgtcgta tgcgaacatc gttgctagca agcccgctcc caaggctgag
     9541 aaaaatgaaa gattttctcc tgcttggatc ggaaacagtt ggaagtctac tccttcttgt
     9601 cggaacaaac cgaaaatgac gatcacaaac aaaggaataa gcttgcagac aagcgtaatc
     9661 gactgaaaag cccctccagc tttagaacct aaaaaattga tcaacaggat cgatacagca
     9721 gctgtaacag ctacgggaac aatcattgac tgggaaagac caaatagatt gacgaactgt
     9781 gtaccgaaaa tgatcgacaa ggctgctaca tttgctggaa aataaatcac gacttgcgcc
     9841 cagcccaata aaaaagctgc tgtatttccg taaatccgtt cgatatattt aatcatccct
     9901 cctgtttctg gaatagcagc tgccagttca gctcctgtta gtcctgcaca gacagaaatc
     9961 ataccaccga gaaaccaaga aaacatatgc aaactggcgg atcctgtaac ttctgctacg
    10021 cttgcggctt taaaaaatac ccctgcaccg atcactgtgc ccataacagt cgataaagca
    10081 gtaaaaaatc ccatcgtacg ttttaattca ttttctattt ttacttcttt tactggcgtg
    10141 gcttgatatt cttccatccc ttgtcccctt tcataccata gccaatccca aaagtataaa
    10201 aaactttttg gatgtggttc ttgttttata ccgtctctac caatttttct ttcagttttt
    10261 ccaacatatc ctttgtcatc acatctaagt cgtatttcgg cgaaaagccc cattcctctt
    10321 gcgcacaaga aacatctagt gaatttggcc aagattctgc tattgactga cgtacaggat
    10381 ccacatcata atccatttca aaatctggta tgattttttg aatagatgct ttgatctctt
    10441 ccgggtcaaa agacatcgcc gatatattga aggcatttcg atgaaccaat cgatcaggat
    10501 ctgcattcat gagttttata atggcatcga tcgcatctgg catatacatc atatccattg
    10561 atgtaccttg atcaatgaaa ctagtgtact ttccttcttt gattgctgaa taataaatat
    10621 cgactgcgta gtctgtcgta ccgcctcctg gcaaggttgc ataagaaatc aatccaggga
    10681 aacggacacc acgagtatct acgccatatt tcacataata ataatcacat agtagttctc
    10741 cagctacctt cgttacccca tacatggttg taggacgctg aagagtatct tgtggggtat
    10801 gatcttttgg agtatttgga ccaaacgcgc cgattgaact tggcgtaaaa aatttcagct
    10861 ggaattctct agcgacttcc aatgcattga ccagcccagt cacgttcaaa ttccaggcta
    10921 attgtggttt tgcttctgca acagctgaca aaagagcagc caaatgaatc aatgtgtcca
    10981 cttgatattg ttctgctatg ctgcgcattt tttcgtaatc cattacatct aggatctcaa
    11041 agatatcgtt tttctcaatg acttcaggca tgcgtatatc tgtcgcaatg acattttcac
    11101 tgcctagttc ttgacgtagc cgtgtgatca actctgaacc aatttgtcct aaacatcctg
    11161 tcaccaatac ttttttcatg atgatcctcc ctgatctttt ttactactat cttcagatga
    11221 ttaacaatat atgcgtgaac atgttctcta attttaaata gacgaaaaaa ctaaatttag
    11281 ttaaataatt tccatttctt ttcctatttt ttgataaact gctactgctt catcaagcat
    11341 ttccctcgta tgttccgcag tcggcatatt tctgatcctg cctgtcccaa gaggcacagt
    11401 tggataaacg attggtttag cataaatccc atgttcgatc agcttcttag agaaagtctg
    11461 agtcactttt tcgtctccca aaatgactgg agtaataggt gtttcagaca taccgatgtt
    11521 atagcctact tttttcaatt cttctttaaa ataattagca ttttcccaaa ctttttcgac
    11581 aagttccgga tgttcttgca tcaaagtaat cgaagccaat gcagctgcag ctgcacctgg
    11641 agttagcgaa gtagaaaata ggaatggccg acttctagct ttcaaccagt cgattagtgt
    11701 tttcgatcca gctacataac ccccaacaac acctatagct ttcgataacg tgcccatttg
    11761 catatcgatt ttgtcgctta atccaaaatg cttgacggtt cctgcacctt ttccagttac
    11821 gcctgagccg tgcgcatcat cgacgtaggt gatcagtcca tatttttcaa caatcggaat
    11881 aatttccggc aatcgtgcaa tgtctccatc catggagaaa acaccatctg tgatatacat
    11941 gattttttta tatttcttac tttctatcgc ttcctttgct tttttctcaa ggtctttcat
    12001 atcttgatgt ttaattcgga tgatttttgc tccggataag cggcaaccgt caataataga
    12061 tgcatggttt aattcatccg ataagatagc atcttctttt gtcattactg cagagatagc
    12121 gcccatattg cagttgaatc cagattgaaa ggcgattgct gcttctgtcc ctttgaattc
    12181 tgcgattttt tcttctaatt gctgatggat tttcagcgaa ccattgatcg ttcggactgc
    12241 tcctgctcct acaccataag tttcggttgc ttcaatacat gcttttttca attcttcgcg
    12301 attggcaaat ccaagataat tgttggatgc tagattgatc atcttttttc cgtctacgat
    12361 gatacaagca ccattttcac tttctagtat gtcgatcgta ttgtataacc ccttttcttt
    12421 cagttgatcc aattctttct ctaaatacat tgttagttct ttggaagaca ttgtctccac
    12481 ctccatattc atctgtcttg cagaacatac caaacatata tcgtgtcatt catctacaaa
    12541 gcaagtatgc tatatttttg ataacgctta cactgattat gagaattatt taaccacatt
    12601 ttttatttac agtatctttt tactttcaaa tgacttatat agtatgaaca attacctttt
    12661 agataatgat ctattttact ctacctgggc agataaatac aacacaacca ttgatagaag
    12721 ccctttcgtt aaaatcaatt gtattctgaa tatttaattt atttattgca tagaccaata
    12781 aaaatacata aaatttccat cttcgtttat cagcaatttt taattttact ctctgaatat
    12841 ttcttaaaag ttttacttgt tgaattc
//
LOCUS       DAZULG010000079        12735 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000079 DAZULG010000000
VERSION     DAZULG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.081"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG9CY_002017"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG9CY_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4938519.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG9CY_002018"
     CDS             complement(323..892)
                     /locus_tag="ACG9CY_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS4938520.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG9CY_002019"
     CDS             1078..1980
                     /locus_tag="ACG9CY_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4938521.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG9CY_002020"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG9CY_002020"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS4938522.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG9CY_002021"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG9CY_002021"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS4938523.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG9CY_002022"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG9CY_002022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS4938524.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACG9CY_002023"
     CDS             8548..8733
                     /locus_tag="ACG9CY_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS4938525.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACG9CY_002024"
     CDS             complement(8795..9991)
                     /locus_tag="ACG9CY_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4938526.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACG9CY_002025"
     CDS             complement(10161..11597)
                     /locus_tag="ACG9CY_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4938527.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACG9CY_002026"
     CDS             11890..12699
                     /locus_tag="ACG9CY_002026"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4938528.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2673 c         2250 g         4010 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgaca
//
LOCUS       DAZULG010000080        12640 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000080 DAZULG010000000
VERSION     DAZULG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.082"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1226)
                     /locus_tag="ACG9CY_002027"
     CDS             complement(<1..1226)
                     /locus_tag="ACG9CY_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4938529.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVP"
     gene            complement(1263..2129)
                     /locus_tag="ACG9CY_002028"
     CDS             complement(1263..2129)
                     /locus_tag="ACG9CY_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4938530.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(2218..3243)
                     /locus_tag="ACG9CY_002029"
     CDS             complement(2218..3243)
                     /locus_tag="ACG9CY_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4938531.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(3261..3935)
                     /locus_tag="ACG9CY_002030"
     CDS             complement(3261..3935)
                     /locus_tag="ACG9CY_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4938532.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYSYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(4119..5012)
                     /locus_tag="ACG9CY_002031"
     CDS             complement(4119..5012)
                     /locus_tag="ACG9CY_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4938533.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(5086..6783)
                     /locus_tag="ACG9CY_002032"
     CDS             complement(5086..6783)
                     /locus_tag="ACG9CY_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4938534.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVASNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(6776..8089)
                     /locus_tag="ACG9CY_002033"
     CDS             complement(6776..8089)
                     /locus_tag="ACG9CY_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4938535.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(8089..12030)
                     /locus_tag="ACG9CY_002034"
     CDS             complement(8089..12030)
                     /locus_tag="ACG9CY_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4938536.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(12139..12597)
                     /locus_tag="ACG9CY_002035"
     CDS             complement(12139..12597)
                     /locus_tag="ACG9CY_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4938537.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         3405 a         2909 c         2171 g         4155 t
ORIGIN      
        1 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
       61 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
      121 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
      181 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
      241 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
      301 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
      361 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
      421 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
      481 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
      541 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
      601 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
      661 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
      721 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
      781 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
      841 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
      901 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
      961 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
     1021 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
     1081 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
     1141 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
     1201 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
     1261 gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa aaggcaacca
     1321 acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct
     1381 aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat
     1441 ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt
     1501 ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc
     1561 attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg
     1621 atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa
     1681 tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac
     1741 gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc
     1801 cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac
     1861 aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca
     1921 acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga
     1981 ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt
     2041 tgttgattgg catcgccacc aattttacca cttactaaac caatactacc ccatgccaac
     2101 atagggacta atgctattaa aatttccata accaatctcc ttttatttca catgataaga
     2161 aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta
     2221 acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg tcccttttcc
     2281 ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata
     2341 aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt
     2401 atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag
     2461 gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata
     2521 tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa
     2581 aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc
     2641 ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc
     2701 ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt
     2761 tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg
     2821 aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata
     2881 atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat
     2941 cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata
     3001 attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct
     3061 caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc
     3121 aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc
     3181 aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt
     3241 catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata
     3301 tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat
     3361 gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt
     3421 aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa
     3481 tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg
     3541 tggctcatca tttcactaga taattctgtg tagctgtaag aacggcgaag taatgcattg
     3601 atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg
     3661 ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact
     3721 ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata
     3781 tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg
     3841 gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg
     3901 atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt
     3961 gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt
     4021 ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc
     4081 agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt
     4141 tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat
     4201 atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt
     4261 gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc
     4321 acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat
     4381 gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga
     4441 ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt
     4501 tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt
     4561 ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca
     4621 tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt
     4681 tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg
     4741 acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc
     4801 actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc
     4861 tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa
     4921 actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg
     4981 gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact
     5041 gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt
     5101 cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa
     5161 gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac
     5221 tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata
     5281 agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc
     5341 catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt
     5401 tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc
     5461 tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc
     5521 ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt
     5581 ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc
     5641 aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc
     5701 tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc
     5761 taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac
     5821 aaacccgttg cttgctactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc
     5881 ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat
     5941 tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat
     6001 aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt
     6061 ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt
     6121 catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta
     6181 tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg
     6241 catcataaca accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa
     6301 gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa
     6361 taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa
     6421 aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc
     6481 gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac
     6541 gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa
     6601 tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt
     6661 cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat
     6721 ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt
     6781 catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat aaaagaattc
     6841 ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac cattttcctg
     6901 attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt tcttttacgg
     6961 tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact ttcccggatt
     7021 ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct tttgcatcca
     7081 cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc tcgtaacctt
     7141 ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt tgtcctgtcg
     7201 taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc aaaggatcat
     7261 tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata tctaaataat
     7321 atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat ggttttcctt
     7381 tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt agataaccga
     7441 catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc gggtgataaa
     7501 ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc aatccaactt
     7561 ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg tttttcttag
     7621 gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc ccagttgtta
     7681 ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata ttgtttctgt
     7741 tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca gctttttttt
     7801 catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct
     7861 gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca tcattttcat
     7921 ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc aacacgaatt
     7981 ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg actgctgcaa
     8041 gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt accttgctcc
     8101 ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg ccatgaagaa
     8161 tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca acggattttc
     8221 aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa gttcatattc
     8281 taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg aaatctcaac
     8341 ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt caggttcttc
     8401 tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt caggtgactt
     8461 cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata gggaagaatc
     8521 gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac tattttgttt
     8581 ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa ttttcaaaag
     8641 ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac catcgatgat
     8701 ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt caatgataaa
     8761 cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat acccatttgg
     8821 tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt ggtcgtaatt
     8881 taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga tcccaggcaa
     8941 acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca gcgggaagga
     9001 agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc cttcatggtc
     9061 taattttcta actcggatct cccgataatc aaacgtattg taagcagtga atgctaccca
     9121 taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt cacctgtttg
     9181 cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg tgtcattttc
     9241 atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt ctgcatagtt
     9301 ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg gatgaataac
     9361 tttctcacca tccacgatta catatttccc gtcttcatct actgaataga acgtgagtct
     9421 ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg gatatccgat
     9481 ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga ttggcatatt
     9541 actgccttca attagtttat tgatattcat gtggattggc ttgaattcat ttcgcatttc
     9601 cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt gtacaacaga
     9661 aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg gtccgccgac
     9721 tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct catcttcgtt
     9781 ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat aacgtggtgg
     9841 tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat ctcctaaaat
     9901 cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg tttcgattgg
     9961 ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc cagccaacgg
    10021 ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt cgtaacgaat
    10081 ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat cctccaggtt
    10141 ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc ccgcatcaag
    10201 ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg gagaaaattt
    10261 ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt ctggtggggc
    10321 ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc cgttttctcc
    10381 tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg gtgagacatt
    10441 aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat
    10501 atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc gcggtgttac
    10561 gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag catctagtcg
    10621 ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac ttggcgggtt
    10681 ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg cagatgactt
    10741 ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac caacccagtg
    10801 agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat ttacttcatc
    10861 ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg cattagaagt
    10921 attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag caatcttatc
    10981 ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac ttgactgaat
    11041 cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt tttctggatt
    11101 tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa gccctagtgt
    11161 aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg
    11221 attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt tattgatagc
    11281 tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg ggtcagagag
    11341 cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag tttgactatt
    11401 ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa cgatatcgat
    11461 tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt aaagggttgt
    11521 tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt ctacccattt
    11581 tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt catttcccca
    11641 aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg atccattatt
    11701 agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac tatcatcgat
    11761 caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat tgctttgtgt
    11821 ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcgggtagc gatgctgttc
    11881 gttttcaact tctccgctag gatagatata ttcatagggg tcttgcgtag ctgttggccc
    11941 aagacgctcc aaaataccgt tgaataactg gcgttgaatg cgctggagtg cttgaccgct
    12001 gggtacgatt ggttcgtcca gcttttccat ctcattttct tcgctacgtt gaatttcagc
    12061 ggaatcttct gtatcagttt cattttcttg taattcttgg ttatctattc tttcatcaac
    12121 caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg tttcaaaaat
    12181 caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt cttgaccatc
    12241 gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac ctgttccacg
    12301 aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac gtattctacc
    12361 ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt cttctttttc
    12421 aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat cctcctttgt
    12481 ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact ggaaatatgg
    12541 tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc ctttcacatc
    12601 cttatactcc tttcaaaaat atttgtcttc tttcacaaca
//
LOCUS       DAZULG010000081        12243 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000081 DAZULG010000000
VERSION     DAZULG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.083"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..1268
                     /gene="mltG"
                     /locus_tag="ACG9CY_002036"
     CDS             93..1268
                     /gene="mltG"
                     /locus_tag="ACG9CY_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS4938538.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            1404..1883
                     /gene="greA"
                     /locus_tag="ACG9CY_002037"
     CDS             1404..1883
                     /gene="greA"
                     /locus_tag="ACG9CY_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS4938539.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            2052..2783
                     /gene="liaF"
                     /locus_tag="ACG9CY_002038"
     CDS             2052..2783
                     /gene="liaF"
                     /locus_tag="ACG9CY_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS4938540.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            2780..3847
                     /gene="liaS"
                     /locus_tag="ACG9CY_002039"
     CDS             2780..3847
                     /gene="liaS"
                     /locus_tag="ACG9CY_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS4938541.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            3854..4486
                     /locus_tag="ACG9CY_002040"
     CDS             3854..4486
                     /locus_tag="ACG9CY_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4938542.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            4626..5291
                     /locus_tag="ACG9CY_002041"
     CDS             4626..5291
                     /locus_tag="ACG9CY_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS4938543.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            5319..5612
                     /locus_tag="ACG9CY_002042"
     CDS             5319..5612
                     /locus_tag="ACG9CY_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS4938544.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(5680..6627)
                     /locus_tag="ACG9CY_002043"
     CDS             complement(5680..6627)
                     /locus_tag="ACG9CY_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS4938545.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(6805..7608)
                     /locus_tag="ACG9CY_002044"
     CDS             complement(6805..7608)
                     /locus_tag="ACG9CY_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS4938546.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            7754..8668
                     /locus_tag="ACG9CY_002045"
     CDS             7754..8668
                     /locus_tag="ACG9CY_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4938547.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            8716..9186
                     /locus_tag="ACG9CY_002046"
     CDS             8716..9186
                     /locus_tag="ACG9CY_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS4938548.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            9271..9879
                     /locus_tag="ACG9CY_002047"
     CDS             9271..9879
                     /locus_tag="ACG9CY_002047"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS4938549.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            10013..10213
                     /locus_tag="ACG9CY_002048"
     CDS             10013..10213
                     /locus_tag="ACG9CY_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4938550.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            10967..12193
                     /locus_tag="ACG9CY_002049"
     CDS             10967..12193
                     /locus_tag="ACG9CY_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4938551.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         4217 a         1860 c         2515 g         3651 t
ORIGIN      
        1 ttgatagaat atttaaataa aatcattcac gccaattcaa atatcatgta taatgaataa
       61 agttacgaac ttgatagtaa ggaggagttg ttttgtcagg aaacaaacaa cacccaagta
      121 aagataaaaa agaagccagt aaggcagcta cagcagatat aatcgacaag cggagaaatc
      181 tgcgtaaaaa agaagataag attgtgagga agatcatttt agtgattgct cttacattat
      241 tgattattgg cggatttctt ggatttaccg tgtatcgtta tgtagatagc gggttgaagc
      301 ctttagataa atcagatgac caattagtcc aagtcgagat cccgagtggc tcttctaaca
      361 agcagattgg tgagattttg gagaaggaca atattattaa aagcgggatt gttttcaatt
      421 attatacaaa atttaaaaat ttaactggct ttcaagctgg ctattatcag ttagcgccta
      481 atatgactct tgacgagatt ggcaaacagt tgcaagaagg cggtacttct gagccaacaa
      541 aagttgcaga tgggaaaatt gccattccag aagggtacga tatcgatcag attgctgaac
      601 gggtagcgaa ggtaactggt aaagacaaga aagaattttt agatttagtg aatgatgaga
      661 ctttctttaa cgagttgcac caaaaattcc cagaactgtt ggaaagtgca tctaaagctg
      721 agaacacaaa ataccgtttg gaaggttact tattcccagc cacatatgat tattacacga
      781 gtacttcgct taaggatcta gtgattgaaa tggtgaacaa gacgaatacg gtcatgcaaa
      841 actattattc agctatcaag cagaaaaatt tgactgtaca agaagtttta actctagctt
      901 ctctagttga aaaagaaggc gtaaaagaaa atgatcgtaa aaacattgca caagtcttct
      961 ttaaccgaat caaagcaaat atgccacttc agtcagatat ctctgtttta tacgcattag
     1021 gtgaacacaa agaattagtg acgtatgaag atacagctgt agattcacca tataatttgt
     1081 ataccaatac cggctatgga cctggacctt ttgacaatcc aagcgaagag gcaatcaagg
     1141 cagtattaga accagcagaa aatgactatt attatttcgt agcagatacg agcactggaa
     1201 atgtttattt cgctaaaact tatgaagaac atatggaatt agtggaaaaa tatgtaaata
     1261 attcttagaa aaacaggctc tagacattga tcatttttag aaaaacattt acatttgaag
     1321 aaaagcgtgg taatcttttt tggactatat tcctcaaaag attacctttt gcggtatttt
     1381 atcgaaaaaa ggagacgtta cacatggtag agaaagttta tccaatgact ctagaaggta
     1441 aagaaaaatt agaaaaagaa ttagaagaac taaaaacagt caaacgcgga gaaatcattg
     1501 aacgaatcaa aattgcccgc ggtttcggag atctttccga aaactctgaa tatgaatcag
     1561 cgaaagatga acaagcattt gttgaaggaa gaatcacaac aattgaaaat atgattcgtt
     1621 ttgctcaaat catcgataac gacggtgtag atgcagatga ggtatcaatt ggtaaaacag
     1681 ttacattcat cgaactacca gatggagaag aagaagagta cacgattgtc ggaagcgctg
     1741 aagcagatcc gttttctggc aaaatttcca atgattctcc tattgcgcaa gcattgattg
     1801 gcaaacgtaa aaatgatgaa gtaactattg ctactccagg tggcgatatg ttagttaaaa
     1861 tcgtcaaagt agaacaagcg tagtctgact gaaagatcta gagagttcat cttactgatg
     1921 atacactcta gatctttttc tctttgtgta ttttagattt caacaaacga tcttacatct
     1981 aaagtcctat agccaacagt taaaaaaata ggtatgatga aatgagaatg tgtcaggagg
     2041 gagaaagagc gatgaatagt tcttggcgtt tttttgtggt agttgaagca ctactgctgt
     2101 tatttgctgt ttggcaaatc gtaaacaata ctggactttt gcttttagtg atattcggta
     2161 ttttcaatat atatctagct atgcgcaaac atcctcgtac taaatttcaa aattttcaat
     2221 tgatattagg aagtttggtc atttttttta gcttagtcaa cagtcctgct ttatggatga
     2281 tggctgtatt tgccgtttta tttattggtc tgaaaggtgt agagatttca gggatagatt
     2341 taaccaaaaa tgctttttgg cgaaagaaac agatcatgat gatccaaaca gagcaattaa
     2401 aaacacataa taatgagcgg aaaagacagc aattattcgg taaccaacgt attggaaacg
     2461 acgtctatga atgggacgat atcaacatcg ccgtcatttc tggtgatact atcatagatt
     2521 tgggcaatac gcttttgcca aaagatgata atattgtgat tgtaagaaaa gggataggca
     2581 gaacacggat cttagtacct ttgggcgtag ccatccgatt agagcatgcg acattagtag
     2641 gaaatgtctt gtttgaagaa gagcaatttt ctttgaaaaa tgaacaaatc aaaatctaca
     2701 gcagtgatta tgatgaaaac cctcgtagat tgaagattat tactaatacc ttgattggag
     2761 atgtcgaggt gatacgaata tgatgggaaa aatatccaga gcgatgctag ctgtttattc
     2821 ggggattgcg gctttcctta ttattctatt ttcacttttc acttattttt atgccagcaa
     2881 tcaaagtcat tggtggggag aattactgcg tgcacgttta ttatatgttc cgcttatctt
     2941 ccatttgtta gccatttcct taggtgtagg attaattgtc tttctattat tatcacttat
     3001 tcaaaagaca aaatacggga aaatcgaaga aaagctgcgt gcactttctt ctggcaatta
     3061 tgaatccaaa ttgttgcttc ttccgatccc aagtgcatcg gacgatttat acatcaaaga
     3121 tattgataag gaaatcacta agataaaaga aaaaatgatt gaagtatcta gtgaattaca
     3181 aattgtaacg agccgtcccc aatatgtaga tggacaaacc aaagaagaaa ttttagaatt
     3241 agaaagacat cgattagccc gtgagctgca tgattcagtt tcgcaacaat tgtttgcagc
     3301 aatgatgatg atgtcggcat tgacggagca agcagaaaaa agcgagacac cagaaatgtt
     3361 tcgcaagcag ttgaaaatgg tagcagagat catcaacgct tcccagtctg agatgcgtgc
     3421 gctgcttctt catctacgtc cagtcaattt agaagagaaa agtttaaagc aaggtatcga
     3481 gcagttattg aaggaattgc aaaataaaat ccagatttcg ctgaaatggg atgtagaaga
     3541 tgtaaaacta tctagttcca ttgaggatca cttgttccga atcgttcaag aattgttatc
     3601 aaatacatta agacatgcta aagccaatga attagaggta tatttgcaca aaatagacaa
     3661 taatcttttg ttacgtatta ttgacgacgg aacagggttt aatatgaatg aaacgaaaac
     3721 gggaagttat ggattgaaca atatcaaaga acgagtagct ggtattggtg gaacagtaaa
     3781 aatcattagt tttaaaggtc aaggtacaag tgtagaaatc aaggtccctt tgatgaagga
     3841 ggcatagata aggatgataa aagttttatt agtagatgac catgaaatgg tgcgcttagg
     3901 cgtctctttt tatttatcta ttcaagaaga cattgaagta attggagaag ctgaaaatgg
     3961 acgacaaggc tatgagaagg cgatgacact tcgaccggac gtcattttaa tggacttagt
     4021 catggaagaa atggatggta tcgaatcaac aaaagcaatc ttaaaagatt ggccagaagc
     4081 taaaatcatt atcgtaacca gttttattga tgacgaaaaa gtttatcctg ccattgaagc
     4141 aggtgcagca gggtatttgt tgaagacctc taccgcacat gaaattgctg atgctataag
     4201 ggcaacgcaa cgtggcgagc gagtattaga accagaagtg acaacaaaaa tgatggaaaa
     4261 aatgagtcgg cgaaatgagc ctgtattaca tgaagaacta acgaatcggg aaaatgaaat
     4321 tttaatgctg atttctgaag gaaaaagtaa tcaggaaata gcggacgaat tgtttattac
     4381 actgaaaaca gtaaaaacac atgtttccaa tatattggct aagctagaag tggaagaccg
     4441 tacacaagcc gctatctatg cattcaaaca tggtttggtg aaataaaaac gatgataaag
     4501 aggataatca aaagctgaga aaataactat tatttttctt ggctttttta caataatgat
     4561 gcttgctttt gtttttgtaa aggctttgtt atactgagaa aagaagatac taaaggagga
     4621 aaacggtgaa acaaaatttt gcaattgtag gtcttggacg atttggcgga agtatctgcc
     4681 gaacactgat cgaagctggt caagaagtac tggctattga tagtaatgaa gatcgggtca
     4741 atgaatatat gaatatagcc actcatgctg ttgtggcaaa tgcacaggat gaaatgacac
     4801 ttcgatcatt aggcataaga aatttcgatc atgtaattgt cgctattgga gaagatattc
     4861 aagccagtat cttagttact ctgatggtaa aggaaatggg cgtaccaaat attttagcaa
     4921 aagcccaaaa tgagtatcat gccagagtac ttgaaaagat cggagctgat cgcgtcgtcc
     4981 atcccgagcg agacatggga ataaggattg cgcataattt agtgtcaaag aatattttag
     5041 attacctaga gttgtcagat aaatattctt tagcagaaat ccgagtctcc aatcctaaat
     5101 ttttcaacaa aacattagct gaattaaatt tcaggcaacg ttttgactta acagtagtcg
     5161 ccatacgaag agtagatcaa acggttatcg cttctcctgc agcagatgaa tatgttcgtg
     5221 aaaatgataa tctactggtt attggagaga caggcgatgt ggatattcta gacgataaga
     5281 tgaatcaata gaaagaaaag agaaagtagg ttgttgggat gaaattaacg gtaacgccaa
     5341 aagcacaaaa gtggtttgac tcagaaattg atctgccaga aacttacgga atacgttttt
     5401 ttggaaaagt ctacggcaaa acagaggttc atgatggttt ttcgattgga atgtcagttg
     5461 aacagccaga acatcctgta aagaaagtgg aaattgatgg gacacttttt tttatcgagg
     5521 aagcagatga atggttcttt aaaggatatg atttacttgt tgattatgat cccaacctgg
     5581 aggagccaac ctatcacttt aaaaaacaat aaaaaaagag agggtgacac aatttttgtc
     5641 accttctctt ttttcttttg aaaatattgt tttcagctat taatatcctg cagacaattt
     5701 tacttgattg gtttgaagct catgggtggc aaagtagctt ttcagattgg ttaaaaaaat
     5761 agccattaat ttcttttgaa aatcaggtgt caaacctgca atgtgggaag taatcagtac
     5821 attgtccatt ttccaaagag gatgatcttg tggaagaggc tcttcctcaa aaacgtctaa
     5881 tgctgcaaag gaaagttgct ggttatttaa agcctgcacc agatcttgtg tcttaacaga
     5941 agcaccacgg ccaacattga tgaatacaga ttttggatca aagtgttcaa acatatgctg
     6001 atcaaagagc ccagttgttt cctccgttag aggtaagata ttgactacga aatctacttc
     6061 aggaagaact tcctttagtt gatttaaaga aaaagtcttt tccattcctt ctgctggatg
     6121 accggatgta ttgattccat agcagttcat gtctaatgct ttgcattttt ttgcaatcat
     6181 ttttccaata tggccagttc caatgattag tatttttttc ccttggatag attggtaatg
     6241 gaccgatgaa cctaaccatt tcatgtcact ctgtgccttt tgtgcttgaa aaagaccacg
     6301 agaataccca aggatgattc ctaagacatg ctccgtaatc gattcactgt ggatgccact
     6361 accatttgaa agtaaaatat gtttctttga aaaagtctca aggggtagct gatcaacacc
     6421 agctgagata gattggaccc atttcaaggt agaagaggct aatagttttt cttggtagtc
     6481 tgagttccag cccaaactga tctctacatt ttgtagatcc tctggttgta attctgtttt
     6541 aaagatataa ccaggcgcta tagtatggat ttcttcgata aattcatcac gaaatggacg
     6601 atctgcataa atgattcttt ctgacatagt taatttcccg cctttcttta ctctaagtgt
     6661 agcgcataca agaagaactt tgaagcgcct tgcttgtttt ttgagaaaga aatatttttc
     6721 aaataaaaaa ggggctgtga caaaaaaatg tcacaacctc taaaagggtc tattgaagta
     6781 gaaataacga gttgtcttta tttcttaatt gatcgatatt tttgcccatc ctttgacagc
     6841 ttgagcaaaa ataaagattc ctgcaaaagc aatgaaggcg ctagtgtcaa aagaaggaag
     6901 aaagaccatc aagattgttg caattatagt aggcaatgaa attgaataga tcaatgtttt
     6961 taaacaatca agaaaagtag cttttgttag tcgaaagcgt gcatagagaa aagcagcaat
     7021 gttggtaaat agcaaagtga tcagcagatt cagaaaacta ggataaatag aaaatataaa
     7081 tgtcaggact tttatccaaa gtggtataga tgcttcactt aaaaaagaac ggagctgctt
     7141 acctgttaag ttttttaaag attcattggt gtagggaagt tctaattggt tattgttcaa
     7201 caaagcagaa gtcgtgcctg tattaggaag agctatgata agtttgtttt ttaacatccc
     7261 gacactaaca aaattcccca tcaaatcagt agagatgtcc tgttctgttc gttttccctc
     7321 tggatcaaat gtgaagataa tggagtttgt ttggtaaatg aatccttctt tttcttcggt
     7381 ttgaagctgc ccgttttgga ttgtaaaatc aggaatgcga gtagcaatct tttgcccatc
     7441 caattgaatg ttgtttaaga cttgaaaaac ttgaaaagag attggcaaag ccataattat
     7501 actcaaaact aaaagataga caatggcttt ccaaaatggg acatttctcg cctcctttag
     7561 gtctttgaat cgtataaagg aactgataat taattgtttt gttgtcataa gtactcctca
     7621 tctagtagtg tcatcacttg caattctagc gaagtatcaa gtgaaataca agtgctttaa
     7681 ggaaacagct gaatgattgg tgaaatatca aaaaaatgtt attcttgtct aaagactatc
     7741 caatataaat aaaatggagg aaggaatagt gcctacacag ccacagagat catcaaaaaa
     7801 actatttgga gtagtggttt tgaatgcggt gatcacttta tcagaattta ttggagggat
     7861 tatgtcaggg agtttgtctc ttgtttcaga tgctttccat aaccttagtg atacattggc
     7921 aattattttt tcttacgggg cacaaaaaat ggcccgaaaa gaagcaaata aaaaacgtac
     7981 gtatggctac cagcgtttag aaatattgag tgctttcatc aactcattca tattgatcat
     8041 attatccttg tttttaaccg ttgaagcatt caagcggttc acttcaccag aaaagataaa
     8101 tagtcatttg atgctaacag tagcagttat tggattgctc gcgaatttgt tctcagtatt
     8161 attattacga caagaagcag atgaaagttt gaatataaaa tctagttatt tgcatttgtt
     8221 aagtgataca ctgtcttcgg taagtgtgat tatcggagca attttgatcc gttttttcga
     8281 tatctactgg atcgatccag ttattacact gatcatttcg atatatatat tgatagaagc
     8341 gataatggtg atcaaaaaag ctgccgctat tttgatacaa agtgcacctg ctatcgacta
     8401 tgaaaaaatg gaacaagaaa ttgaagcaat cgatggggta aaggatgttc atcatgtcca
     8461 tatttggcag tatagcgaaa agataattat tttcgacgga catattgatt ttgaagatca
     8521 gctactaagc gagattgaaa agatatatcc tcgtataatt tctttattaa aattaaaata
     8581 tggtattaca catgtgacga tccaagcaga gacaaaggca aaggatcaaa aaaaattaat
     8641 ttttttaaat aaagatgaaa gttattgaaa tatagagtat gataaataag aatttttaat
     8701 aaggtaggtt ggctcatgaa gcagtatcga cagttaaaag gatatttaga agcaaaagga
     8761 tattgggaga tactagtcta tctctttttt ggtggtttgg ctacgattgt aaattttgtt
     8821 agttttgctc tcgcacgcca atattttgat ttaagtatgg ccttatccaa ttctatttcg
     8881 tggttttgtt ctgtattgtt tgcttttgta acgaacaaac tttgggtatt ccattcaaaa
     8941 tcaccgaatt ttacacatgc acttattgaa tgcggtaagt tcttcttcta ccgtatcctt
     9001 tcttacggat tggacatggg ggcaatggta ttgttgatca atgttatgaa cagcaatgag
     9061 tatgtggcga aaatcatcac tcaaattatt gttatccttg cgaactatat tttcagcaag
     9121 ttgtttattt ttaaagaaac tgaggttttt gaagaagaag taggtcaaag tgaaaaagac
     9181 gactaggcat aacaattgaa gaatttcctt ctctttgtta ctctttgtaa tgtagacatt
     9241 attaagaaaa cattgaggag gaattttttt atgacttaca cattaccaga tctaccttat
     9301 gcatatgacg cattggaacc ttatattgac gaagaaacga tgcatctgca tcatgataag
     9361 catcacaata cttatgtgac gaatttaaat tcagcaattg agaaataccc agaattaggc
     9421 gaaaaaacaa tagaagaatt attatctgat atggacgcta ttccaacaga tatcaagaca
     9481 gctgtacgta acaatggtgg cggacatgct aaccattcat ttttctggga aatcatggca
     9541 ccaaatgctg gtggcgaacc tacaggagaa ataaaagaag cgattaatga agcttttggt
     9601 gatttttctt cttttaaaga agaattcaaa aaagcagccg ctggacgatt tggttctgga
     9661 tgggcttggc ttgtaatgga aaatggaaaa ttagctatta cctctactgc aaatcaagat
     9721 tctccattga tggaaggaaa gacaccaatt ctaggtttgg acgtttggga acatgcttat
     9781 tatttgaaat ataaaaatgt ccgaccagat tatattgcag cattctggaa tgttattaat
     9841 tgggatgaag taaacaaacg tttcgaagca gcaaaataac catttcctcc gtcatatcag
     9901 tcttaaaaat aatttggaaa ataaaagagc gaaagcttgc tagcgttttc taaattgggt
     9961 ataatgttct tgcaaggtta cttgcaagaa cttttttgga ggatatacat ttatggaaca
    10021 cggaacggtt aaatggttca acaatgaaaa aggatttggg tttattacag tagacggcgg
    10081 agatgacgta tttgttcatt tctcagcaat tcaaggagac ggcttcaaga gcctagaaga
    10141 aggtcaagaa gtagaattca cgattgttga aggtgcacgc ggacctcaag ctgctgaagt
    10201 gactaaatta taataatttg ttgaataaag acacgacttc ttagagagtc gtgtcttttt
    10261 gtctatttat gaagttttta gattttttgg ctaagtaaat gcatttgttc acttgacatg
    10321 tcctaactaa ttatcaagca aggaacattt tcttcgttag taatggattt taaagaaatt
    10381 tcagtttttg gatgttgaat aaaagaccat ttgtgaaacc actagtatac atactcatta
    10441 ccatgtcaat tctattactt taaaagaaaa tcgttttact ttgaagagga gcatatctat
    10501 ccattaaaag atgatggtaa tgcaatcact agttcgagtc attttaagag ccatgcagtg
    10561 agctggaaat aaaacgaata gaagatgggt ttaacaaaag gaccagtcat aggaagaaat
    10621 tgtcgtgaaa tcgaatgata atctgaacgg taggcaaaga ctgttaaaaa gatgggaaag
    10681 gtatgtgcga tcattttact gcacgattat ttggaaaata ccttgttgtt cgctttttct
    10741 ggaaaaaata cataaaaaaa gataaaacga ttgcgaactg cgtgtattcg gtctttcgat
    10801 ctttttcacg gtatggtatg atagttaaga attttgagaa attttaaaaa ataataatcg
    10861 ttacacaaaa atgtttttta aagttttctt atgattaaaa agtgaatttg ctgattgaac
    10921 agttatatga gacaatattt tagaaaatgt gaaacagagg tgtattatga agcgatggca
    10981 aaaagttcta ttaaccttgt taggggtatt cgttttgctg attgccggat tatcagcata
    11041 cggaatcaga tttatgggag aagcgagcca aacagtaaac aaaatttcta aaaactctaa
    11101 tcgaatatcc tccaaacgtt ctgagagagt aagcattgat gacaaagaac ctttttcggt
    11161 tttgctatta ggtttagata ccggtggatt aggaagaacg gaacaaggac gttctgatac
    11221 gatgatggta gtaacagtta atccgaaaca aaaaaaatca acgatcatca gtttggaccg
    11281 agatatttat acgaatatcg taggttatgg aacggtagat aaattaaatc atgcctacgc
    11341 atttggtggt gtcgaaatgg ccatggattc aattgagcaa ctgctcgata ttcctattga
    11401 tcattatgta acgatcaatt tggatggcat ggaagatttg atcaatgcgg tcggtggcat
    11461 caaagtgaat aataaaattg actttacttt ggatggtgtc catgtaccag taggtgaaca
    11521 aacattgaat ggagaaaaag gtttagccta ttctcgaatg cgacacgaag atccagaagg
    11581 cgatatcggt cgacaacgtc gtcagcggga agtagtgacg aaaatcgtca ataaagtttt
    11641 aagtttagat ggcgtaagca attatagaaa aattttaaaa gcagttgaga acaatgtgac
    11701 gacggactta gattgggatg acatgcttga tgtagcgacg aactacaccc cggcgtttga
    11761 aacaatcaaa caagaccagt tgcaaggcga aggacaaatg atcaatgata tctactatca
    11821 aattttagga aaaaatgatc ttctttcgat ccaaaatgaa ctgaagaaac aattaaatat
    11881 aaaaacaagc gatacattac ctaatttgaa aaatgagaat gcttcaatga tgttctttga
    11941 tgattcggaa gatggcgatt cgacaaacgg tgatggatcg aatagtgaag ggtacaccga
    12001 ttattcttcc tattcagaaa acacatatga tcagtaccaa gatccgaata cctattatca
    12061 gcaaacagaa gatacaagtc agtactatac accaagtcaa gatactcaaa gttatgatcc
    12121 ttacgctgga caggaacaaa atggttatgg ttatgaccag aataatgagt atggaacaac
    12181 aagtgggtac tagaaaaaaa tagtttggtt aataaagagt gccacctaaa ttagacaaaa
    12241 aat
//
LOCUS       DAZULG010000082        12144 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000082 DAZULG010000000
VERSION     DAZULG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.084"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACG9CY_002050"
     CDS             <1..566
                     /locus_tag="ACG9CY_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS4938552.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /gene="fic"
                     /locus_tag="ACG9CY_002051"
     CDS             967..1572
                     /gene="fic"
                     /locus_tag="ACG9CY_002051"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS4938553.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="ACG9CY_002052"
     CDS             1588..2160
                     /locus_tag="ACG9CY_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4938554.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="ACG9CY_002053"
     CDS             complement(2412..2669)
                     /locus_tag="ACG9CY_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938555.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="ACG9CY_002054"
     CDS             complement(2795..3610)
                     /locus_tag="ACG9CY_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938556.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="ACG9CY_002055"
     CDS             4127..5452
                     /locus_tag="ACG9CY_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4938557.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="ACG9CY_002056"
     CDS             5445..5795
                     /locus_tag="ACG9CY_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938558.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5777..5962
                     /locus_tag="ACG9CY_002057"
     CDS             5777..5962
                     /locus_tag="ACG9CY_002057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938559.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACG9CY_002058"
     CDS             complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACG9CY_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFS4938560.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="ACG9CY_002059"
     CDS             complement(7113..8066)
                     /locus_tag="ACG9CY_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4938561.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="ACG9CY_002060"
     CDS             8678..10171
                     /locus_tag="ACG9CY_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFS4938562.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="ACG9CY_002061"
     CDS             10306..10602
                     /locus_tag="ACG9CY_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS4938563.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="ACG9CY_002062"
     CDS             10703..11554
                     /locus_tag="ACG9CY_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938564.1"
                     /translation="MKSDDWIEHNWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11787
                     /locus_tag="ACG9CY_002063"
     CDS             11575..11787
                     /locus_tag="ACG9CY_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938565.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4172 a         1863 c         2268 g         3841 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca actggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaa
//
LOCUS       DAZULG010000083        12121 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000083 DAZULG010000000
VERSION     DAZULG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.085"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..568)
                     /gene="frr"
                     /locus_tag="ACG9CY_002064"
     CDS             complement(11..568)
                     /gene="frr"
                     /locus_tag="ACG9CY_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS4938566.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(571..1293)
                     /gene="pyrH"
                     /locus_tag="ACG9CY_002065"
     CDS             complement(571..1293)
                     /gene="pyrH"
                     /locus_tag="ACG9CY_002065"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS4938567.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1429..2310)
                     /gene="tsf"
                     /locus_tag="ACG9CY_002066"
     CDS             complement(1429..2310)
                     /gene="tsf"
                     /locus_tag="ACG9CY_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS4938568.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2409..3191)
                     /gene="rpsB"
                     /locus_tag="ACG9CY_002067"
     CDS             complement(2409..3191)
                     /gene="rpsB"
                     /locus_tag="ACG9CY_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS4938569.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3550..4029
                     /locus_tag="ACG9CY_002068"
     CDS             3550..4029
                     /locus_tag="ACG9CY_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4938570.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4246..5337
                     /gene="phnW"
                     /locus_tag="ACG9CY_002069"
     CDS             4246..5337
                     /gene="phnW"
                     /locus_tag="ACG9CY_002069"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS4938571.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5330..5458
                     /locus_tag="ACG9CY_002070"
     CDS             5330..5458
                     /locus_tag="ACG9CY_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS4938572.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5462..6007
                     /locus_tag="ACG9CY_002071"
     CDS             5462..6007
                     /locus_tag="ACG9CY_002071"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFS4938573.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6461..8152)
                     /gene="argS"
                     /locus_tag="ACG9CY_002072"
     CDS             complement(6461..8152)
                     /gene="argS"
                     /locus_tag="ACG9CY_002072"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS4938574.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8572..9519)
                     /gene="arcC"
                     /locus_tag="ACG9CY_002073"
     CDS             complement(8572..9519)
                     /gene="arcC"
                     /locus_tag="ACG9CY_002073"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4938575.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9634..10653)
                     /gene="argF"
                     /locus_tag="ACG9CY_002074"
     CDS             complement(9634..10653)
                     /gene="argF"
                     /locus_tag="ACG9CY_002074"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS4938576.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10744..11973)
                     /gene="arcA"
                     /locus_tag="ACG9CY_002075"
     CDS             complement(10744..11973)
                     /gene="arcA"
                     /locus_tag="ACG9CY_002075"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS4938577.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3311 a         2518 c         2193 g         4099 t
ORIGIN      
        1 tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc ttatctactt
       61 ctttgatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc aaatcatctt
      121 cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca cgacggatgt
      181 tacgaatcgc gatttttgca ttttctgctt cttttttaac atctttagct aattcttttc
      241 tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca ttggttggac
      301 tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa
      361 aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt tggttcaatg
      421 gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc
      481 ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc attttttctt
      541 ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg ttccgatatt
      601 ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga caagcagtgg
      661 aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta atcctttggc
      721 aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta ctttcggatc
      781 agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg cgccgatttc
      841 tgccgcgcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc
      901 aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa tatatggctc
      961 tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa
     1021 cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt aatcagcttg
     1081 agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc cgccgcccac
     1141 aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga tgatttcttt
     1201 gattactggt ggtttgattc caaatcctgc gtccccagct aaagcttctc cactcaattt
     1261 taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat tttctattgc
     1321 cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg ttttgcttaa
     1381 aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt attttttcac
     1441 ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt cgccaacttc
     1501 gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt caacagtcat
     1561 atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga atttcttcaa
     1621 gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca atgcttgttc
     1681 tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt cgtttacata
     1741 acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt
     1801 tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc caccaaatgc
     1861 agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt ctccgataac
     1921 ttgagttgct tcgatcaagt cagattcaat tgtaccttta tctgttttga ttttcattgc
     1981 agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt ttactaaatc
     2041 ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa cgattgctgc
     2101 agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt catttttctt
     2161 cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca tgtcgccttc
     2221 tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt cgcgtagttc
     2281 ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt
     2341 aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa cagctccatc
     2401 attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat ttcttcgatt
     2461 gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc accttggttt
     2521 ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg aattgcatcg
     2581 tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc aaccatagct
     2641 acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca
     2701 acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa gaatttttca
     2761 agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac ttcaaaagtt
     2821 ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg gatagtatcc
     2881 cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc tgcacgagtt
     2941 gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg
     3001 ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt aactgttttt
     3061 tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt catttttgga
     3121 ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg tttcatagaa
     3181 attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt atcttctacc
     3241 ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg atttgctgat
     3301 gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga gcaaatatac
     3361 ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag aaaaacagta
     3421 gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt ataaaagacg
     3481 aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct ttcgtaaagg
     3541 agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat tgaagaattc
     3601 ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt taccgccacg
     3661 caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat
     3721 ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag gactgaggaa
     3781 gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg agtccacttt
     3841 ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt tttagatgag
     3901 atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat ggtcatctcg
     3961 gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac tgaagtatca
     4021 cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat tttcaatatt
     4081 ttccttattg aataaacctt tacactcttt acaaaaatac atcatttttt attcaaattt
     4141 ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa caagagcagc
     4201 ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta taaattatta
     4261 acttctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt
     4321 acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt attggatctt
     4381 gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcag ctccttcggg
     4441 gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat ttgttcaaac
     4501 ggcgcatata gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt
     4561 tatgaggtat cggaacatca aatcctccaa gctcatgtga ttacacagct gattgaagag
     4621 aatccgtcta tctccgcact agcaatggtc catagcgaga ctacttccgg tattttgaat
     4681 aatttggaag agattgctgc tgtcacacaa gaaagagatc ttttgtttat agtggacgcg
     4741 atgtcaagct ttggtgggat accgatttgt gtagccgaac tagggattga ttttttgatt
     4801 tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact tgctaataaa
     4861 gcggaactgg aaaaacgaaa aaatgttagc cgaagtttgt cacttgatct atatgaccag
     4921 taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac ccatacggtt
     4981 ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga aactcgatat
     5041 tcacgttatc tcgccaataa tcatgtgcta cgacaaaaaa tgacagagat ggggttccag
     5101 cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc taaggactgg
     5161 cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa atggatttgt gatctatccc
     5221 gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat ctatccggaa
     5281 gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa tgattaaaac
     5341 tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc ctgtccacgc
     5401 ttttagaaat gcttttctag aaaaaggaat ccaactgact gacaaagaaa tccgctaacc
     5461 gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt ctgtcaaaaa
     5521 acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg aattgtatca
     5581 acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga aacccgacac
     5641 attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca cgaatggatt
     5701 taatgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaaaggt attcaccaga
     5761 cttcatggca acttttgaag acgtcgaagg gtatggtcga ccgtttcctt atatgatcca
     5821 taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac
     5881 ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg taattgaagg
     5941 tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg ggaaaaagat
     6001 cgtttaaaac agtctgttgc tcttagattc caacaagccg gcgctgatat ttgtgtaaac
     6061 aatttaaaag agttaacaga atacattttg tacgctgaca tgaatccaga atgactttta
     6121 tacaaaataa gaggttgtga aaaagctgtc ctgtatcaag taataaaaac agccaagcaa
     6181 aaatagctcc tcatattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa
     6241 caccgtttat tcgaaaaaaa aaagggagct gtgacacgac ttttgtcaca gtccccttat
     6301 ttgcaaacaa acagtgttta agagacaacc tctccactaa acggtgttca ttcagctgac
     6361 tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt ttgtttctct
     6421 aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta tctggtgcat
     6481 gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa actagagcta
     6541 agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag tatttgttga
     6601 atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat ttttccccag
     6661 cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag ctggtatcgt
     6721 cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc aacaggctga
     6781 ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat cgaacaactt
     6841 cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg aaaatgactg
     6901 ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg tttttctcgc
     6961 tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat aagacgatct
     7021 ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat ggaatatggt
     7081 gcatgtcgtc agaccagtca aagcccattt ccttcaagac agctttcaac tgtttaaaat
     7141 gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa gtacgtttac
     7201 ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa
     7261 tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct ttatcctcgt
     7321 tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca ttgtagaaag
     7381 cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta ttgaattctt
     7441 tcatcgattc atcacggaac cattgccata attgaatagc ctctttatcg ccttcttcca
     7501 gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca gctacttcat
     7561 ggaattggac gtataaacga agcaattcat tgattggttc tgctttaaca gcttcttctg
     7621 taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag tcacctaaat
     7681 ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc gaattaccga
     7741 taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga gaagacatgt
     7801 cgattggtac tgttccttga ttcccgatgt tgctatcccc ataatgttct ttttctttca
     7861 ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag ttcaagtaag
     7921 gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct aaatctgcag
     7981 cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga aatgcaacat
     8041 ctccgtgttc agctgatttt ggattttcta gtaattgctc gacttgttcc agtgttaaat
     8101 catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc atctttattc
     8161 ctcctaaagt gttcccttga ttgttaaaca aaaaaaatct ctagcgacgg catacttagt
     8221 atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta aaaaaggacc
     8281 agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt
     8341 cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa gaatgataca
     8401 agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta tatcagaaaa
     8461 aaaaacgaaa ggctagtcgt ttcataaaaa aacaatccag atggcctaaa ggagaagaaa
     8521 taccacctgg atagctataa atcatttcgt aaatacgaaa gacttaacaa attaatcagc
     8581 tacgataatc gttccttctt cagaagctaa aagattctcg atattttcta aagaagtgat
     8641 gattgcctta gcgtttggtt ttgcttcaac aaatgcaata gctgcttcta ctttcggaag
     8701 catgcttcct ggagcgaatt gcttttcatc aatgtattgt ttcatttctg aaacagtaac
     8761 agtttctaat ttcttttgat ctggtttttg gtaattgaca tacacattgt ctactcctgt
     8821 caatacgatc agcaaatccg catcgatgat ttcggctaat ttttctgaag caaaatcttt
     8881 atcgattacg gcttctcgtc cttctaatcc agtagctgtt tctacaacag gaatcccacc
     8941 gccaccaaca gagactgtga taactccttg atcgaccagc gtttcgatga cacgtgcttc
     9001 tttaatcgag atgggtttag gggaagcgac tacttttctc catccacgtc cagcatcttc
     9061 tttgaatgtc acagtggagt cagcattcat ttgttctttg gcttcttctt ccgtgtagaa
     9121 tggaccgact ggtttacttg gatttttgaa tgaaggatca ttttcatcca caataacttg
     9181 agtgactaaa gaaaccacat ctttatcgat ccctttttcc ttcagtactt cgcccatggc
     9241 gttttgtaac cagtaaccga tagatccttc tgtcattgca acacatgtat ctaaaggcat
     9301 tgccggtgtt ttctcagaat cagcagcttg ctgctgaatc aataggttac cgacttgcgg
     9361 accatttccg tgggagatga tcaattcatc tccttgttcg ataaatttca ctaaatactt
     9421 tgctgtttcc attaacgctt cttgttgggc ttttgcactt gcatccgtag acaagatggc
     9481 attgccccct aaagcgacaa ctacttttcg tttggccatt tcattcgtcc tttcttcagt
     9541 tctaccgaat tttctagata acaaaacgag ccagcgtttt gtgattttcc ctcaatgctg
     9601 actcgttttt tcgtgagatg aatgtggtta tgcttaaacg cgagggataa acaagttacc
     9661 taatgttgct gccatgatcg ctttgattga gtgcatacga ttttctgctt ggtcaaattg
     9721 acgagcatat ttgctgcgga agacttcatc tgttacttcc atttcagtga tgccaaagcg
     9781 ttctttcatt tgttcaccgt aaacagtgtt tgtatcatgg aaagctggca agcagtgcaa
     9841 gaagatcaaa cgatctgtgt tgtgtgtttt ttctaccatt tccatattga tttggtaagg
     9901 tttcaacaat ttgatacgtt cttcgaattt atcttcttca cccattgata cccatacatc
     9961 agaataaagg acgtctgctc cgtctacacc tttatcaaca tcgtcggtaa tcatcaattg
    10021 tgcaccggat ttttcagcaa agccttctgc cattttgacg atttcttctt caggttgtaa
    10081 ttcttttggt gcaacgatac gcatattcgc tcctaaaatt gcacctgtga ctaataaaga
    10141 gttagccata ttgtttcgac catctccgca gtaagcaaca gtgatacctt ctactgttcc
    10201 aaagttttct tggatagtta aaaagtcagc gatcatttgt gttggatgcc attcatcagt
    10261 caagccattc caaactggaa caccagagaa ttctgctagt tcctctacca ttttttgact
    10321 gaatccacgg aattcgattc cgtcaaacat acgtcctagt actttagctg tatcttctgt
    10381 ggattctttt ttacctaatt ggatatcatt tgcacctaga tattcaggat gtgcacctag
    10441 atcgatcgct gcagtagtaa atgcagaacg agtacgtgta gatgtttttt caaataaaag
    10501 cgcaatattt tttccttcta gatagtgatg tgggatacca cgttttttca agtctttcaa
    10561 gtgttctgaa aaatcgatca aatattgtaa ttcttctttt gtaaagtctt tttctgctaa
    10621 taagcttctt ccttggaata ctgattcttt catattaaac attctccttt agattgtttt
    10681 tatttaagat taaagaggaa atcccgacgg cagctgacag taagactgcc gtcaggaaac
    10741 aaattacttc aagtcttcac ggacaagtgg ttggctcatg cagcgtggac caccacgacc
    10801 tcgtgacaat tcacttgaat tgatttctaa cactttgatg ccatagctac gaagcaattc
    10861 gttcgataca taatttcggt tataagtgac tacaacacct ggagcgattg ccaatgtgtt
    10921 tgatccatca ttccattgtt ctcgaggtgc aacgatctca tctccattac cagttgggat
    10981 cagtacaaga tcatctaatc caagagcagc tttcatcgtt tcgtgtaagt tatctgaatg
    11041 agtaatcttg atatcatcac catctggttc gattgtaaag acatccatct taccgtcttt
    11101 actttgaatt gctggatgga tagtgaattt atcgtgatct accattgtaa atacggtatc
    11161 caaatgcatc attgcacggt tgttcggaat tttgattgcc aacacttttt caaatcctga
    11221 attttttgcg aataggtggc gtgctaattt ttctaaagct ttcgcattag ttcgttgaga
    11281 aatcccaaca gctactactt tatcgcttaa gatcaattcg tctccgcctt cgatatgatc
    11341 tggattttca cgatccaacc atacttcgat tcctttattt gcaaatcttg gatgatgttt
    11401 caaaatatat tctgtaaaca tcgattcacg acgacgtgct tcaaaagtca tcttattgac
    11461 tgtcatccca tttcccattg atgcggaagg gtcgcgtgta aagtacaagt ttggcattgg
    11521 gtccatgtaa aatggatagt catcgtctgc tgacagatca tatagactgc tagaacgtac
    11581 ttcgatatct tttgtacgaa ccccagccat gattttatct accatttctt gcgtttccat
    11641 tgatagaagg aactcatgaa gaccctctct tacagcattc gatgcaatat gagattcatc
    11701 caacatttta ttcaagaatt gttctttgac attgcctgcg tcaatcgctt ctgccgctaa
    11761 tttttccaag taaagagtct cgacgccttc attttgcaat gtcttcgcaa agttgtcatg
    11821 ttcttcttga gcaatcggaa gatatggaat atcatcgaac aacagacgat ccataatatc
    11881 tggtgtcaaa ttttccactt cttgtccagg ccgtttcagt aaaactgtct ttaatttccc
    11941 tatttcagag aaaacgtgaa taggtttatc catgttgtgt tgcctccttc tttttgatta
    12001 catgtatagg atatcctcct tttgtaatca ctttcataat tttgtctcat tcaaaatgag
    12061 acattcctca cattaatatt cgataattaa ataattaaac gtttttctat ttttaagcgt
    12121 a
//
LOCUS       DAZULG010000084        12020 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000084 DAZULG010000000
VERSION     DAZULG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.086"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..976)
                     /locus_tag="ACG9CY_002076"
     CDS             complement(65..976)
                     /locus_tag="ACG9CY_002076"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4938578.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1353..1991)
                     /locus_tag="ACG9CY_002077"
     CDS             complement(1353..1991)
                     /locus_tag="ACG9CY_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938579.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1995..3977)
                     /locus_tag="ACG9CY_002078"
                     /pseudo
     CDS             complement(1995..3977)
                     /locus_tag="ACG9CY_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4157..4822
                     /locus_tag="ACG9CY_002079"
     CDS             4157..4822
                     /locus_tag="ACG9CY_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938580.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5219..7252)
                     /gene="brxL"
                     /locus_tag="ACG9CY_002080"
     CDS             complement(5219..7252)
                     /gene="brxL"
                     /locus_tag="ACG9CY_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFS4938581.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7370..9892)
                     /gene="pglZ"
                     /locus_tag="ACG9CY_002081"
     CDS             complement(7370..9892)
                     /gene="pglZ"
                     /locus_tag="ACG9CY_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFS4938582.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(10008..>12020)
                     /gene="pglX"
                     /locus_tag="ACG9CY_002082"
     CDS             complement(10008..>12020)
                     /gene="pglX"
                     /locus_tag="ACG9CY_002082"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFS4938583.1"
                     /translation="SVFIKLNSQMLIDNGYASFMTPFVWMFISSYEKLRSFLINERNI
                     SSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFKGGMEVQKIKTLEAIHQHG
                     ECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTNDVADAKQGLATGNNNKFL
                     RYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKWYGNNEYVVNWENDGDEIK
                     NYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQNQAIFETKGSVLFSKDITD
                     FEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVTSERKLKKIREKVDENIDI
                     ARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLVNQFNTLKSNEEELNDIFI
                     NHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCIFGRYSLDEDGLIFAGGEW
                     DPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVEITFGKETLEENLQFIADT
                     LGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGKQNGFKALMYLHRYTPDQL
                     GKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTKEIVKIQKQLKEVREYDEK
                     LGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKGVKEPKK"
BASE COUNT         3800 a         2305 c         1731 g         4184 t
ORIGIN      
        1 aataaaaaga tttagatgaa cagttttatt gatttaaata gttcatattt tttatttata
       61 ccgcttattt actacttgct gtactagaat ctgaagtgaa tgtactcaaa acattttcaa
      121 agtctttgtc tttgattttt acattagcgt ctttcaatac ttcgccaatt actttattct
      181 ggaaggtact gtcacttaat tttgtgtctg ttgcaatttc tttcaattct tttttgtatt
      241 tatccatgtc gttgcctttg ttttggtttt tcaccatttt aaccacataa tattccgtag
      301 tataagttga agcattggta gaagtgatca catcagaaac ttgaccatct ttcaacttaa
      361 aggctgcttc tttgacttct gccggaaccg ttgtagtagt ggaatcaaat ttcacttttc
      421 cgccatcttc ttttgtcgta gtatcagttg acttgtcttt ggctaattta gagaaatcgt
      481 ctcccttatc agcagctttc ttcacatctt ttgcatcatc ttcacttgct acttggataa
      541 tttgagcttc cacttctgga tggaaagatt cccaagccgt ttttaaatct tcatcggtca
      601 atttgatatg tttctttaga ccttcttgaa aggctagact ttgtttgata ctgtctttga
      661 atgtttgttc tgtgtaacct gcagatttca attgtgaatc aaatgaatca cccaattgtt
      721 tctttgtttg atcatattgg gaatcgattt ctttggttga tacgtcatct ccatatttat
      781 cttcaaatac ttgataaacg atcaactgtt gcacagcgga atctgctcct tgcatttttt
      841 tcaagctatc cattacttct tgttcgctga ttgtaccgcc tttataacta acggctgttt
      901 tttcaccggc attcgtacag cctgacaata agccagtgcc tagtacgatg gcaagtgcta
      961 aaaatctttt tttcatttat caattctcct acttttcgtt ttttgcgaat tgctaaaaac
     1021 aaaggtgttc aagatgaaaa tcatcaagaa cacctttact aatctgctcg atacgtcctg
     1081 aatagatata aggctacaca atccagaaag ttcgctcagc agaataaatg tcaagtagca
     1141 catcacacct taatatacta cttcttttgt taccgttttt caagtctatc ttgcgttccg
     1201 tatttaacgt aactaattct tagaacaaat gttatcgatt ttgtttttct tcttccgctg
     1261 ttgggatatt caaatcagac acttttatgt tcgattttcc agacattttt ttgttgtttt
     1321 tattagaaaa agtttgtttt ttcatttgtt ttctaaagca tgatcttctt gtttcctatt
     1381 ttggtcagat atggatcatg cgtgaccacg ataactaacg taccttggga agcctgttgc
     1441 gataagattt caacgacttg ctccttattt cgtttatcta atgatccagt tggttcatcg
     1501 gctaaaatga ttttgctatt cttcaataag actctcgcac aggcgattct ttgcttctca
     1561 ccaccagata atgtgtagat aggcgtactt agcttcttgt ttatatttac ttttttcaat
     1621 gcctcatact ttgctgttcg cttatctgaa acatttaaat tcgaaaactt caatccaata
     1681 tctaaattaa aatcgattgt ttcatattca atcaacccaa aatcttgaaa aagaaaagag
     1741 agggcatttc tttttatcaa gcgcttctct cttccagtta aatgttttgt ttcttgatta
     1801 aataaataaa attgccctga actgaattga tcattcaatc ctaatatatt caataaagta
     1861 cttttccctg ctccgctctc tccaactaca gtcacaaaat ccccagcatc cgcagtgaaa
     1921 cgatccaaat gaagaatcgt tttgtttcga atgatttttt tcgcaccttt tatcactatc
     1981 aacggctcca attgttaacc tccattcatc agcaataact gacttttctt ttctgtagac
     2041 aaaataaaaa caactgaaag aataaaatca atgaagctaa agaaaaaaat attaaaaaga
     2101 tatttaggct ctctttctat aagaaaaagg aagattacac ttgtcgttcc ccatttgatg
     2161 aggttcatca tgaagaaatc tttatacgtt tcaaagagcg aataaccact tgaacgaaag
     2221 atagcgattt tccgacaatt gtagacaaaa tatatgcata acatgacata gctcaacaag
     2281 aagaaagaaa agaaagtgag taaaaatatc tgtatcaagt aatgaaatga tctgcttaac
     2341 actttgacag ctatctcttc agtctgagta tacggaaatg ataactgcaa gttatcacgt
     2401 aactgacttt cttcaatcag ctcaaataac ctttctttcg acaaagagcc tatcttgatc
     2461 tttaacggtg gatagatttc tccgctgaga atatttcttt cccaatacgt actgtttttc
     2521 aatgtcagta tttcaacaac cggataatgt tcgatccagg ggatagaagg aataaatgta
     2581 taaatgggtt gattgttttt tataaataga aatttcggtt ctttttcact gatttcttga
     2641 taatattctg ttatttgcgc caggtcattt tttaaatctg tatgtctata tcgttcaggg
     2701 atcaatataa cagggtgcgt ttctttttcg gttatttgga tcaaccttcc agagacatca
     2761 atcaaaggaa atttcttaag ataattcaga tttattttta tccctctgcc aaactgttca
     2821 ttttcttcat tcaagtaatc tgatacattg actaatatac ttccgttttt attcaaagtt
     2881 tcatataatt cctgatcggc tgctgatgca aaacgattgg aataaataaa atccacgtgg
     2941 tttttcccga tggttactgg ataaaataca tgatagtcct caccagcgat ttcttttggt
     3001 gtttcatggg aaacagctaa ctgtgaagaa gaaaaaacga tagttgctaa ttcgacattg
     3061 gcaacggtca aaagaagaga aaacaacaat ccaaatgtaa aaagaatgta ttggatatag
     3121 ttgaacgctt ctcttaacga agcgattttt tcaactaata aaagagaaat caaagagata
     3181 ccctatattc cgctgaacaa gaaaacgtac atctggaaca aataaagaaa ttgctcctta
     3241 taatacataa aaataaaacc accaatgatt cctgcaatta tgaatgtaat aatgattttc
     3301 tttttcccca ataaacacca caatacttga tgtaatgaaa agccattatt caacaataaa
     3361 ctgatttctt tattcatcaa taagaaaaag gatgataaca aaaggaataa aagaatcaat
     3421 ccaatagtta aatacactcg ataattatat acgtctgtac ttgaaataac cgaatacgaa
     3481 tagctatttt caaagtcttc aaaaatatta gtggcataat cagtgtgaaa tgcttgattg
     3541 aatcgttcca caaatagtaa aatgaaccgt tcataggctt ctttttctct cgtcatcaag
     3601 aaaaattcac cttcaaaatc ttttattttg atcgattctt caatggacgt tacgcgaaag
     3661 gaggtattag aatagggtac cttctcgtga gaagatttga catccccttg ataatacaat
     3721 gtcatttcaa gctttggctg tatcttgaag ttccaagatg tatctacttc attggattca
     3781 tttactgttt ttttcatata agaaagctca gtttctgatg agacattttc aaataccatg
     3841 attagctgct catattcttc ttgcccaata aaatgttgtg gcaatcgaat cggagtataa
     3901 gtatctgttt gatggaaaaa ttcatttgtc cgatagagta cctgtttatt tataataacc
     3961 aaggcaaaac cactcatgat accggtcact atgattgtga gtaaaactgt gaactttttt
     4021 ttgattatca ccactctccc ttacttaaat gattctttga taaaatcata ccgtttttca
     4081 tcaaaaaaac gtatctatca aatgtatagg tcatgtagaa taaatctata tactgtagaa
     4141 tcgaaaggag aagaagatga acatcggaga aaaaatacga acttaccgca gaaattgtaa
     4201 gcttacgcaa aaagaattag gcgaaaaaat cggtgtttcc gataaaacaa tctctagttg
     4261 ggaaaattct cgtacaatgc cagaccttga aatgctgtca cttttacatc agactttagg
     4321 tctgccgatt gattttcctt ctattgaatc agctaccgaa tcaatagaac caacattgcc
     4381 ggatgaacct cttgttatca agaaatctta ttcatcaaaa caacccttct cactcaaaaa
     4441 gacactatct ttctttctgc tcgtcttttg tttggtctat ctttttctta tcaattatca
     4501 atataaaaat gaaacggttg atcgattcaa tccttttttg aaaccacaaa tcggctatac
     4561 cacactccct gtaggatacg tggaaaattc catcgaacat tgggtatgta atgatatctt
     4621 tgttcatggt acatggctgt cttttcacgc tggagaggta gaaaaaggta agaatcatgc
     4681 tgtaatcaaa cacaaaggaa aacacgtcat gaacgttcgc ctgctcactt gggaagccat
     4741 tccctcagat taccaagaaa tcctcatcca agactatgac gatgccttaa aaagaagccc
     4801 tcaggagcca gataggcatt aagtaggaga tgaacgtaga gatagaaaaa atcaatgcac
     4861 atgatcttat caaaaaaaaa caggggtaca aattaattga aaaagctttg agatgaaagt
     4921 tcctaaataa aaaagttcag aatgataggt aaaaaaatga ctaactattc tagtattagg
     4981 ctatttgtat tattcttctg ttatttttat atcaaaaaat ccagaaataa ctaaatatag
     5041 gtatttctgg cttatagtat aatatttacg ccacagcccc caatgattct ttttggaatc
     5101 atgatcaagg acggacatac acaaaaatat tttgcccatt cgatccgaat agattaacga
     5161 ctaatctagt aagtaatata gattggtacg tagtcaaacc agcaaatgga aaagttgtct
     5221 attccacccc aagggcttta aagacagcat cttccggcgt ttgataaaaa atcaaattaa
     5281 aggtacccat taattctggg ggtacagttc caatatcagc agcgcttatc ataggtacaa
     5341 gtactttttt cgctccggca tcttttgcta cttgtaaaca gttagctaaa ttatctacct
     5401 taatcataga accaccaatt gtaatatccc ctaaaataac taaacttggt aatacaggtt
     5461 tatttaagga aactgagcat aaagctatta tagttgataa cgataatgtt tttgtcatac
     5521 caatgccatt catatcttga acatgaacga caaaatcttt tgtcgtcaaa ctaatagact
     5581 gactaacact tccataatta gctcttaaat atcggtatgc cgaatccatc gcttctcttg
     5641 ctccagcatc tgaattgatc ccagtacgtt caaactttcc atttcccgaa gtcatttgcg
     5701 tttccaattt ataggtacct aattgcccag ttttgccata agataccgtg tacaaatgcc
     5761 ctggcttccc taacccttct ggaatcaaag ttcctccacc ttgttcagga acagaaacat
     5821 actcttctcg ctcttcttct aaatcaatgt atgaaaaatt gacatcataa aattccattc
     5881 caccaatttt ctttaattgt tcttttacac gacgacgcat ttcaagagca aatcttagta
     5941 tttcttcgac atcacttttt tcaaatctac catcaggata caacagtttc atcatcccag
     6001 atacaatccg acgaactgca atcgtatccc gctgatttaa ttgagaacca agtttgaaat
     6061 atttatcaaa actatcgcca aaactaactt ttcgaagctc acgcatgact tcagaaaaat
     6121 aatctgtaat gaagccatag tcatttgtaa aaaaatctgg tcgatattta ggaatttccc
     6181 aacctggtaa ataacaatgc atccgatcca aaaaggctga atccgtcccc ataacctccg
     6241 gaaatggctc aaataaattc gaagttttca atattacatc aacgctttga ttaatatttc
     6301 ctacaaaagc cattgatgct gaagcattct tttcttcttt gcctctagca aaagatcctg
     6361 aagccatgta atctttcatg atttgaacgc catcattatc cttaaattta atcccagcaa
     6421 cttcgtcaaa agcaacacag tcccacatac ctacaagtcc cattgtacga ctagccatat
     6481 tatagaaaag gtttgctact gttgtttgtc cacctgaaac aaggattgag tttggtgaaa
     6541 tttctttata tacatgcgat ttacctgtac cacgtggtcc tagttcacag agattaaaat
     6601 tattttcaat taatggaata agtcttgcta aatgaagcca ttttaccctt ttattaaatt
     6661 gtgtaggttc cataccaatg gaccgtagaa gtaagtcaat ccattcattt gtagtaaact
     6721 ccttgcgagc catcttgtaa gcctcaatgt ctaaaccagg catttggatt ggatcaacct
     6781 tttttgcaat aaaagggcta ttccaacgat cattctcatc ataccaatat tccatcgaaa
     6841 caatacacca gatcccccca cctaataatc gatcaaattt tttcggatac tcttcactaa
     6901 tttgaacatt ttttaatccc aggttagaga attctgcttc ataacaatct ttcttttcat
     6961 ttaaatgtac agttacttta tcaataactg tataaccacc caattcacga atttttgctt
     7021 ttactttttc tgcttcatca ggacgcacat agtttcgagc aagaatattt ttaacatttt
     7081 ccacaccttt ttcaaccaat gtttcatcca atgacgaagc atacatacca agtaaatatt
     7141 ccaatacata aacaggtaca ttcgcacctt ctttaatttt ctttgttaaa tcttttctga
     7201 caatctttcc gggaaagaat tcagataact ttaaaatttc ctgttgttcc actagaattc
     7261 ctccatgtgt tcagtcatta taatagcaat taaagtatat catacttaac cagtaggctt
     7321 gataatacaa aagaaaaagt atccacaaaa aattgtggat acctcgtttt tatatatcaa
     7381 aatcaaatga ttctgcaaaa ggattatcta taatgaattt tattcgctcg gtcaaatcat
     7441 ttttttgatt ttttatcact aaataataag tatcttcaac tgtatatcga cgatttacaa
     7501 attcaaaaac aaatttagta aaccgctgat ttgcatcttc tgtttttgaa tccgcaacat
     7561 agatttcttc atttgagata cgttcaccat attcatcttc aaaataaagc cagaaagatg
     7621 cggggtcata aacatcacta attgcatttt tttgatagaa ttctaacgtg gttgataatc
     7681 ctgtaattct gcggttgcta gtcattaatt caattgctac tggttccttt gcattgctac
     7741 ctttggctac cgtaatttct agaacaggga ctagcatttc ttgaatagat gaccctccat
     7801 gaacgtagct ttgtcccccg ccagctttct taaaaatact tgaagtaatc gggacacaaa
     7861 tgtttctagt gtcatcattt cctaaagaat ctcccaaaga gtaatttttc gtaccaatta
     7921 tttcgtattc ttcttcacta agaataaaac gacgttctac acgatctttt tcatttctag
     7981 atggattttc aatcttatct acttcttcaa cgtggcttct tttataaata aatccatgat
     8041 cagccgtaac tataaaacga tagatactgc catcattatg caagcgatta ataagtcggg
     8101 aaatttcgct tatcgctaaa tcacaggcct caaaaactcg attttcattc gagtgatcac
     8161 ctgttctatc cacttgatcg tgataaatat aaatgactga tttgccatta aatacttctc
     8221 gtacctcagc cttattcatc ttaaccacat catcataatt taaagctaca gcatttgcat
     8281 ctcgttgctg gagaatcgca tttcgattgg ataaaccatt cgtttttttc ccatcaatca
     8341 gtacttctgt tccttcattg taagttagtt gattgtttgg taataaggcg gctttaccga
     8401 attcagtaac tgaaggtaat acagaaagaa agctagccat gtttgttgaa tacttctttt
     8461 ctctaactaa cttacgttct aaattcatcc ctaactcata tcgaaaagca tctgaaataa
     8521 taactacagt tttaacagag cgtttcatta cagcttgatt ataaaatttt cgaatggatg
     8581 gacgattctt aaattcaaaa gtttgattcc aaatttgtcc tgattgatca agaaacttcg
     8641 aataatttgc ttcaactaat cgatacaatt ggccatattt ttgcttttct tctatatgat
     8701 cactattcca aataaatttc cgataatgcg tatcaaccca atacatttcc ttctcatatt
     8761 gagttagcaa attatccatg ttcatcggca agcacaatct gtttttatcc acaagataat
     8821 aagcatgttt caataattga tactcataaa tataatccga tgcgaaatgc attttttcgc
     8881 gctgctttaa actatcaaaa attgtttgtc ctgcaatttt cgttgtagtg tcagaagcct
     8941 ttaatcgttc tgcataaaac attaatattt ttgcatgaat ctcagaaaat acgtctgctt
     9001 caattagatc atcaatttct atactgtcta ataatccatc accatcaata gcctgatata
     9061 cttttttaga tagctgttca aagctttcca tgtaacgtgt atcattcata aactgatcca
     9121 tgaaagtaat aatgtttgct ttcaaatgta gtatttcaaa tcctttgaac ttcttcggca
     9181 tctcttgatc aatttgataa taaaaatagt tcaaataaag acacatcgtc agcttttcca
     9241 atgttggctg gctatccgta aagccaaatt tcatttgaat aaattcccaa aatttagctg
     9301 ttaactgata cttatcaagt tcccttaaag gattctccct actttctgta aagcttttta
     9361 gaactacacg cagtaaatca tttaatcgaa tacttttcga tttcaacaaa acagccatta
     9421 tcattaattc aatcgtttct gatttattaa ttgtcatatg cgtactaaat tcaccaaaac
     9481 ttctcatgcg atcctttgca ttaaagaaat tacgatactg tttaactgtc tgatgaaatc
     9541 taagtggaat agccaattca ttcatgataa tcgctaagcg gtctgcacta aattcctctg
     9601 aataattcaa tacactcaac aaatgattat cttcatcttt atcatcccaa cgaggaaatg
     9661 gcgcatagat taaatagtta gtttcgggat cttcatattc caataaacgc ttagttttaa
     9721 attgtgcgtc cttttctaat cgatagattt ttgcattttg aatgaccaat gagtcaattt
     9781 cttcaagaaa atcctgattt gaatcaaacc aaaaaatgat tttcctacat ccataattag
     9841 gaaaacgttg atttaatttt tcttcaattt gttgcgtatt tatatctgtc atcaatctca
     9901 cctaatcgtt ctctttctat accccttata ttgacataat ttggataatt tcgcacgcaa
     9961 tttaagaata aaaaagaaag ttgttagaaa acaactttct tagataacta tttttttggt
    10021 tctttgaccc cttttgcaaa aatttggaat ttctcgccgg ttttaggatc acgctgcaac
    10081 ttatcataat tgacaattac accatcatct aagtctaatt caattcgttt taatgctaag
    10141 tgacccaact tctcgtcata ctcacgaact tcttttaatt gcttctgaat ctttacaatt
    10201 tcttttgtca ttgcatttct ttcttttggg ttatcacttg ttgaaatttg ttgctgacgc
    10261 aattcgattc gattgtcata cgctttttgt agttcgtgta ggtattcagt acgtactttt
    10321 cctaattgat ctggtgtata gcgatgcaag tacattaacg ctttaaagcc attttgtttt
    10381 cctgagtcca attgccaata aattggtcga ttggagtatg tttttaaatg gtctttgtaa
    10441 aaatcttgta cgaagtaacg acgaataatt tctcgacttg tccctttgcc acctaaagtg
    10501 tcagcgataa actggagatt ttcttcaagg gtttctttac caaaagtaat ctcaacgaaa
    10561 tcaatgaaac gattgacaat gtcaatttgg ctatccttga aatactcatc atcggtgatg
    10621 agtaaaacat tatcagtatc aggtttaaat ttttgatagc gggaaggatc ccattcgccg
    10681 ccagcgaaga tgagaccatc ttcgtctagg gagtaacgac cgaagataca acctattgaa
    10741 taagaaataa aatccttaat ttccctcgcc ttatcaggta accttgcaga gatatattca
    10801 tcttgaacct cttttgttaa ttctttttca agagaataat gattaatgaa tatgtcgttc
    10861 agttcctctt cattcgattt tagagtatta aattgattaa ccaactgcct acttagacag
    10921 ttaaatgcct ctgagagatc ttttttaaac atataactta acaaaatgtt tcttctaaac
    10981 tcgtatgacg tttcaagagc attatagtct tttctagcta tatcgatatt ttcatccact
    11041 ttttctctta ttttttttaa ttttctctca gatgtaactt taagaaaagg tagttttccc
    11101 attggtcctt catggtaatc caaggtggga gaaataaaag ttaaaaagtg attaactaat
    11161 ttagagttta catagccaaa tatttcttca aagtcagtta tatcctttga gaacagaaca
    11221 gatcctttag tttcaaaaat tgcctgattc tgcaatctaa aagatacttg tccagatgaa
    11281 agtgtcgacc aagtaattcc ctttttaaaa tagtaattaa gattttgtgg tctagatttt
    11341 aacttcccat tttcattaac ataatttttt atttcatctc catcattctc ccagttaacc
    11401 acgtattcat tgtttccata ccattttcta aaacgaccac ctttagttgt aggaaaccat
    11461 ttttttctag actttttagc gtcctctaat gttttagcat caaactttat tttgttaaat
    11521 tctacttcaa accaataccg caaaaattta ttgttatttc cggttgccaa cccttgtttt
    11581 gcatctgcaa catcgttagt taattttcct tgagcaaaat cagtgattac gttatcgctt
    11641 gcccagtaag caatcggcat tcctgggatt tttttgaagt tgtcttgatt agtactataa
    11701 aaataatcgc attcaccatg ctgatgaatt gcttctaacg ttttaatttt ttgaacttcc
    11761 attccacctt taaaatctga taattttata tagcttccat cttgttcatt tgaatttttt
    11821 atcgtaaacg tacaaattgg aacggttgct tcttcaaaag ctgaatactc catttgaatc
    11881 aaactagaaa tgtttctttc attaattaaa aatgagcgta acttttcata gctagaaatg
    11941 aacatccaaa caaatggcgt cataaaggaa gcatatccat tatcaatcag catttggcta
    12001 ttcaatttaa taaacacaga
//
LOCUS       DAZULG010000085        11430 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000085 DAZULG010000000
VERSION     DAZULG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.087"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..290)
                     /locus_tag="ACG9CY_002083"
     CDS             complement(66..290)
                     /locus_tag="ACG9CY_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFS4938584.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(488..2068)
                     /locus_tag="ACG9CY_002084"
     CDS             complement(488..2068)
                     /locus_tag="ACG9CY_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS4938585.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2469..3845)
                     /locus_tag="ACG9CY_002085"
     CDS             complement(2469..3845)
                     /locus_tag="ACG9CY_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4938586.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3973..5061)
                     /locus_tag="ACG9CY_002086"
     CDS             complement(3973..5061)
                     /locus_tag="ACG9CY_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4938587.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5233..5667
                     /locus_tag="ACG9CY_002087"
     CDS             5233..5667
                     /locus_tag="ACG9CY_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4938588.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5745..6278)
                     /locus_tag="ACG9CY_002088"
     CDS             complement(5745..6278)
                     /locus_tag="ACG9CY_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS4938589.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="ACG9CY_002089"
     CDS             complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="ACG9CY_002089"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS4938590.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7543..8340)
                     /gene="recX"
                     /locus_tag="ACG9CY_002090"
     CDS             complement(7543..8340)
                     /gene="recX"
                     /locus_tag="ACG9CY_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS4938591.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8447..9829
                     /gene="rlmD"
                     /locus_tag="ACG9CY_002091"
     CDS             8447..9829
                     /gene="rlmD"
                     /locus_tag="ACG9CY_002091"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4938592.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10003..10581)
                     /locus_tag="ACG9CY_002092"
     CDS             complement(10003..10581)
                     /locus_tag="ACG9CY_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS4938593.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10903..11373
                     /locus_tag="ACG9CY_002093"
     CDS             10903..11373
                     /locus_tag="ACG9CY_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS4938594.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         3363 a         2293 c         1949 g         3825 t
ORIGIN      
        1 aaatttttgt gaattccagc ttatttcttg gagttaaacg cttctgtctc agcctctttt
       61 tctttctagc tttttgattt ttctcttttc ttttgtactt ttctcttttc attttcagtt
      121 ctacgcgtgt ctttattttt aactttttcc tctttatgga tgattgatcg tactttttgt
      181 gcaatttgct cgtttttgtc ccagtctttt acatctaata atcgacataa tcgtaacatc
      241 tcttcttttt tctgtttttt tctttcacgt ctttgccatt ctgtttccat gtgtcacact
      301 ctcctttctt taatcatatg tacgcaaaat aatttttata tttttttgat tagacatttc
      361 tatgatttta atatagtaaa aagagccgtc acggaaaatc cgtcacggct ctaaacaaaa
      421 tatgagaggc tgtttttgtt tggctattct tattacttga tgcagggcag cttgtgtcaa
      481 gacttcttta catcaaactt tttaatttta cattgggata tttatccgca aaccagcgtt
      541 ctgcaaattg gttctcgaat aagaacaacg gttggtcgaa acggtcacga gccaagatat
      601 tgcgacttga actcatcttc tcatccaaat cttctggatc gatccagcga gcgattttgt
      661 gtcccattgg agacatgatt acttcggcgt tatactcatt taacatacga tattggaata
      721 cttcgaattg tagctgacca acagctccaa tgatatattc ttccgttaga tatgttttat
      781 aaagctgaat cgctccttct tgaactaatt gatagattcc tttgtggaag gatttttgtt
      841 tcatgacatt ttttgcagtc actttcataa acagctctgg agtaaatgaa gggagttctt
      901 cataagcaac ttttagtttt ccttcgaaca atgtatcccc aatctgataa ttgcctgtat
      961 catatacacc gatgatatcg cctgctactg ctttttcgac gttttctctc gaatctgcca
     1021 taaattgtgt gacattgctt aatttcagtt ttttatttgt acgttccaac cagacatcca
     1081 ttcctcgttc gaatgttcca gaacagattc ggacaaaagc gattcggtca cgatgggctg
     1141 gattcatatt tgcttggatc ttgaatacga aacctgaaaa ttcttcctca taaggactta
     1201 ccatctgatc atcttgtgtt ttgtgtccat aaggagctgg tgccaattca acgaaagtct
     1261 ctaaaaaagt ctgtacaccg aagttagtca atgcagaacc aaaaaatact ggtgtctgat
     1321 cgccacgagc aattttttct tgattaaatg catctccagc ctctttgatt agttctactt
     1381 cttctaagac ttgttcatat aaggaatttt cagttagagg atgattttcg ggaagcagtc
     1441 catcttcatc caattctact aatcgttcat cctgataatt ttccggacga taaaactcta
     1501 cacgattatg gtagatatca taaagacctt ctagcccttt acccataccg attggccaat
     1561 tcatcggata agattcgata tctaatagct cttctaattc ttctaacaat tccaatggtt
     1621 cacgaccatc acgatctagt ttgttgataa aagtaaatat cgggatgcca cgttttttta
     1681 caacttggaa taatttcttt gtctgtgctt cgatcccttt tgcactatcg attaccatga
     1741 ccgcactatc cacggccatc aaagtacgat aagtatcttc agaaaaatct tcatgtcctg
     1801 gtgtatccaa gatatttact cgtttccctt gataatcaaa ctgcataaca gaactagtaa
     1861 cagaaatccc acgttgtttc tcgatttcca tccagtctga tttggcaaaa ttacctgttt
     1921 ttttgccttt tacggtcccg gcttgacgga tagccccacc aaacaatagt aattgttctg
     1981 taatcgttgt tttcccagca tccggatggg aaatgatcgc aaaagttctg cgattgtcca
     2041 cttgttgctt taattttgga ttattcataa cctaactcca tttcttccat tcaattattt
     2101 atattttctc taggcacaga ttgtccgatc tcttaaagaa agacaatttc atatagggct
     2161 gtgacaaaag ccgtgtagca gtctcttttt ccgagtaaac ggcgttctag agacagccta
     2221 tcagcaataa gcccacccaa gccaaaaata cgaaaagctg tttttgcttg gctgctctta
     2281 ttacttaatg caggatagct ttttcacaac ctttgtcaac tactacaact gaatcagtat
     2341 agcatggatt cagcgaatga aaaagccacc aaaccataag agtcaaatat ttttttgtca
     2401 gctgactaaa agaaaagacg tgttgcttgt ttattcaagc aacacgtctt tcataaccaa
     2461 gtttccaatt atcttcttgg ctcgtcatct tcgaattctt ttctgaagta tcttcgttct
     2521 tcttcaggtt ccgcttctgt ttcttccgga tcatgaaaaa tgacacggat tttaaggatt
     2581 cgagaacctt ccatttcttc agaaaccagc gtaatattat cgacatcaaa cgataacttt
     2641 tcgccttcat ccggaatcat gcctaatgct gtaatcaaat aaccagccat cgtatctaca
     2701 tcactcatat gcagatcact gtcaaaagct tcattgaact catcaatcag catacgtcct
     2761 tggatgatat attcgtgttc atcgattttt tcataaagat tctcaacttc atctgattca
     2821 tcatcgattt ctccgacgat ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg
     2881 ccgccatatt catctaataa aatagccatc tgattctgcg tctttttcat ttcatataac
     2941 agatcatcaa taaagaccgt ttcaggaacg aataaaggct cctgcatgat tttcttgata
     3001 tctaaattat caaagccgaa tttatgggca gcttttagta aattctttgt atgaaggatt
     3061 ccgactactt tatctttgtc ttcgttataa acagggattc ttgaatagtt ttcggataaa
     3121 acttcattca cattttcttc cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt
     3181 gggaccatga cttctcgggc aactttcgta tcaagagaaa aaaccccttg gagcatttct
     3241 aattcttcat tattcaatac gccttcgttt tcaagcatat aacgcatttc atctctggtc
     3301 atttttgaat cttcatcatc aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa
     3361 gcagaaagca gccagacgaa gggcttagct actacaccaa tcaagcgtat cagccctgaa
     3421 gtgaactttg cgacttcttc tgatttattt aaagcaatac gtttagggta caattctcca
     3481 aaaacaatcg atacataggt caaaatagcc agcacaatga tatttgcaat attttttgcc
     3541 gctgtgccgc cacctaatac cggagctaac cgttccgata aagtattagc tagtgaagca
     3601 ccagacaaaa tgttgaccaa agtgattcct acttggattg tcgataggaa attatttgga
     3661 ttctgcaata tttttaaaag cttttgagat ttcacatctc cttcatcagc tttttgctcg
     3721 attcgattct tattgatcga aacaacagca atttcggaag ctgctaaaaa agcattgatc
     3781 aatgtcaaaa cgatcaataa taaaatctgc gctaacagcg actgactctc agggtcagca
     3841 ttcataatcg cattctctcc ttataaaatt aatttttagt ttacgataga aaaaaacgtg
     3901 gcactatagc cacgttaaaa tggtatcaca aagcaatttt ctttgcaata aaaacaagtg
     3961 tactaaaact acctacgagg tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc
     4021 tttgacgatt ttgattatat aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc
     4081 taagaagata ccaagcaatc cggcaatatt tccagctact agaagaacga taatgatcgt
     4141 taatggatgg attttcagtg atttcccaat tacattagga taaatgatat tcccatccaa
     4201 ctgttggacg attagcacaa ccacacaaca aagtaatgct tttaccggat cattgaagac
     4261 tgttactaga aatgcaggtg ctagtcctag ataaggtcct aaatatggaa ttaggtttgt
     4321 caatcccgca atgaccccaa ataagaaagc ataatccaca ccaattacca tatatcctaa
     4381 aaatgtgaag gtaccaacga ataaacattc gattgcttgt ccgctaatgt aattagccaa
     4441 tgttttattc agttgcccca ataactccac aatttggttt cttctttttt ctgggaaaaa
     4501 ctgttggatg tttggcacta gtttttctcc gtctttcaac atatagaaca agatgaatgg
     4561 tgctgtaatg attactacgg ttgccgaagc gatcgttcca acgatcgaac ctaaactatt
     4621 ggatactcca ctaaggaatt gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa
     4681 atatttattg atatctactt ccttgaacaa aggcaattcg gctacctctt ttaaccagct
     4741 ttctacctgt ttgatgaaat caggcatatt agatgccaat gaggaaattt gctgtactag
     4801 acttgggatg acactaagta aaatccaaat aattgctgca actaaaagga gcagcacaat
     4861 tgcgacagcc catattcttt tgatttttgc tttcatcaac agcttgacaa taggattcaa
     4921 taaatagtaa agaaagcctg caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc
     4981 gatcggttcg aaaataaagt ttatttctga tgaaatcatg atcaatgcag ctatgatcaa
     5041 taattcaagc gaccaaaaca ttagtttaga ctgtctaagt ttttcaaaca atagatttcc
     5101 tcctttcttt cttactagta tatattatat tcgttaaaaa ttcttttatt ctttttttat
     5161 ttttgaaaac ttgccatact ttttatttgt tttattttcc agtatactat acgaaaggaa
     5221 ggtggctaga ccatgaaagt tgtacaaaca aaagatacaa tgagcaatat ttacttagat
     5281 gctgtacgga tcaggcatca agtttttgta gtcgaacagg gtgtgccttt gtctcgagaa
     5341 atagacaaag atgaagccca ttgtatccac ttcgtcctgt actcagataa aaaagaaccg
     5401 caaggaactg tacgtctttt gcctttagaa aacggaaaaa tgaaattaca gcggatggcg
     5461 attctttctg aatacagaca ccaagggtta ggaaaaatat tgattgaaga agcagaaaac
     5521 tttgctaaga atcaaggcta taatacgatt ttattagggg cacagtccac agctgaaact
     5581 ttttacgaaa aactaggata tacagcttac ggcgatcctt ttgaagatgc tggcatgcct
     5641 catatttata tgaaaaagac tttataaaaa tttatttcga aaaaagcaaa aaacagcaag
     5701 cttttgagaa aaatctctca ctggcttact gttctttttt gtgtttactt acgcgacaat
     5761 tgcttgtaac ggttatacca aatatcaata tagccttccg agaacgggcc atctccatta
     5821 ttgatccagt caaccagaat cttcacattt tcttttagga tgaagtcaat atcgttcggg
     5881 taattcatca ttttgctgtg catctcatat tcatcgacat ccaatagtcg tttctcgccg
     5941 tcaggaaata ccttgatatc caagtcatag tcgatatatt tcaaggcctc atcgtccagc
     6001 aaaaaaggtg aagctagatt acaataatag gaaaccccct tttctctgat cattgctatt
     6061 acattgaacc agtatttttt atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg
     6121 cgtccatcag attcagtgac taatgtgtga tcattcacgc cgatcataga gtgttcgctt
     6181 gtttttaata ccatggtatc tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt
     6241 tggatcgtca caaactctcc ttcttttgga actcccatag caacccttcc ttaagcagca
     6301 ttttgtgctt tgtgtttggg tgttcattac accgacatca taattattat atcaataaaa
     6361 atgagctttg cctagaaagg attcctattg tttcgatttt tctctaactg atctaccagt
     6421 ttcatttgga ccttaggaaa aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa
     6481 gttttgttct cggtaaattc ttgttctgct ccctcggttg cccgtccgta aaaaaggaga
     6541 acatgccatt tcagatgact aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa
     6601 acaaagagtt cctgcttttc aaatgggaga ttttgctctt cttctgctac taggtcatca
     6661 aacagatcga tttctggctt tgtttcccat tcttttttca atcgctcata ctcctcttgt
     6721 gtcacttcca tcattggaaa tgtccacata ttcgccagca atttttggct atctctttct
     6781 tcaaaataat acgctccaga atgattttga agagctgcac tgatataata aacgtctttt
     6841 ggctttgctt tttttgtttt tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa
     6901 caaaaggctt ggatcggaca agtctcacac tttggagaag ttggtgtaca gatagctgaa
     6961 cctagatcca tcattgcttg gttgaattca cctggatgct tttcatcaat gattttccgc
     7021 atcgcttcat caaagatttt cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac
     7081 aatctgctga ctactcgcat cacgttgccg tcaactgcag gttctggaag tccaaatgcg
     7141 atacttgcaa tcgcgcctgt cgtatatggt cctattccct tcaatgagct aatttcttca
     7201 ggcgtttgcg gcatttcccc atcaaattct gacatgatct gtttagcagc agcttggatg
     7261 ttccgggctc ttgagtaata accaaggccc tcccatgctt ttaaaagctt ttcttctgga
     7321 gcatttgcta gctcttcaat tgtaggaaac cattccatga aacgataaaa ataatcgatc
     7381 actgtgtcta ctctggtttg ctgaagcatg atctcagaga tccagatacg ataaggatca
     7441 cgattatagc gccaaggcag gtttcttttt tcttgttcat accattgaat gaactgatct
     7501 tgaaattctt ttgtttcttt atctgtccat ttttcccatt ctttattcac tttcctcttc
     7561 cttttccgta atgaatcgct ggatcatatc caaatcaaat cctttttgat ataagctttg
     7621 ctttactttt agatttcttt ttttaggttc aaaccttaaa tttcttcgcc atagtttatc
     7681 tccttgtttg actaaataat catattctat ttcttgatct gcttcttgcg gaagatcagc
     7741 tatcgcttgt tggatcactt cttgagaaaa gcctttcgtc atcaatgttt gttggatttt
     7801 tctcaataat tctttttggc tttttccgtg attttttcca aacgcttttt cggctgtctg
     7861 atgtgcattt tgaacttggt cttcaaatgt atattggctg agcgcttgtt caatgatttc
     7921 aggtttcaaa cctttttgct gcatctgctg cgctaatttc tttggtcctt tgtcacttaa
     7981 gcgcatattc gtgcgcacat aactttcccc atagaccaaa tcatcgacaa ggtccaattc
     8041 ttttaagcgg gctacgattt tatttcgatc ttcttgaagg atttcctttt cttttaagta
     8101 tgttcggatt tctttttccg atcgcaactg ataacttaag taattcattg ctaggtgaaa
     8161 accgaaatca gctccgctac tcttcttgat ctcttggata gtttcttcgt ctaattcccg
     8221 tccttttaat aatcgatagc gtactaatac atcttcagat acttgtaaag cttctccttc
     8281 agataaatct acgcgataga aagggccttt gcctttactg attctcgtaa cggttaacat
     8341 aaacatcctc ctcgaacata tattctctat cataccacag aaaatccccg taatcaggta
     8401 ttatatgtta gaataaacca cagaaaaaag aaaaagggag atacttatgg cagaaacaat
     8461 tgtcaaaact ggacaaacgg ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg
     8521 aatcggttat ttcaaacggt tgatcatatt tgttccatat gcactaccaa aagaagaagt
     8581 actagtgaaa atcacgaaag caactcctcg ctatgcagaa ggagaattaa taaaagtcaa
     8641 aaaaacaagc aaagatcgcg tagtggcacc atgtcctgtt tactatgaat gcggtggctg
     8701 tcaattacaa cacttagctt atcaagctca attggatttt aaaaaggatc tgcttttgca
     8761 agcattggaa aaattcaaac cagcaggctt tcgatcttat caacttttgc cgacgatcgg
     8821 gatggatgag ccttggcatt atagaaacaa agcacaattt cagttacgaa aaaataagca
     8881 gactcaaaaa attgaagctg gtttatacga agcaaactct catcagttag tccctataac
     8941 agactgcttg gttcaagaac ctcgaacaca aaaagtaatg aatactgttg ttcaactatt
     9001 gaataaattt gatgcaccca tctatgatga acggacaaac agtggaatct tccgcacaat
     9061 catggttaga atcggtgtta aaactgggga gttacaagtt gtttttatta ctcgcagtaa
     9121 aaaattcccg caaaaaaacg caatgatccg tgagatcaat gaacgactgc ccgaagttgt
     9181 ttcaatcatg caaaatgtcc aggcacaaaa aacgtctctc gtgatggggg atgaaacgat
     9241 ccacttatgg ggaaaagaag ccattgaaga acaaatcaat gaagtgactt tcgatctttc
     9301 tcctcgtgcc ttctttcaac tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat
     9361 caaagcatta gatgtccagc ctaatgaaac gatcgtggat gcttattgcg gagttgggac
     9421 gatcggctta agtgtagcaa aacaagccaa agaagtacgg ggaatggata ccgttcctca
     9481 agcaatcgct gatgcaaaaa aaaatgcgga aagattagga tatcacaaca ctcgatatga
     9541 agtcgggaca gcagagcaag ttctgcctaa atggttgaat gaaggttttc agcctgatgg
     9601 tatcatcgta gatccaccac gtacgggttt ggataacgca ttgatccaat cattattgaa
     9661 ataccctcca aaaaaacttg tttatatctc ctgtaatgtt tctacacttg ccagagatct
     9721 ggttaattta agtaaaaagt tcaagattga atatttacag tccgtcgaca tgtttcctca
     9781 gactgcacgt tgtgaagttg tcgtgaagat gacgagactc gctgactaaa tgaaagaggt
     9841 tgtgaaaaag tctgccctgt atcaagtaat aagaacagcc aaacaaaaat agctcttcat
     9901 gttttatgaa tactgtactt gtgaccgaag agttaacagg aggacactat ttatttaaaa
     9961 aaagggttgt gacatagttt ttgtcacagc cctttttgat tattattgga tattcacgtc
    10021 tatcacttga taaaatgcat ttgtcgtatc gctgatattc catactgcta aaatcacatt
    10081 atatcctttt cgatcctgcg gaagtgtgat cgtttggttg actgtttttg gtggaacagt
    10141 tgctttgtcg tctatttttg tgatcaattc aaaatcacta aatttcaaag gttgattagg
    10201 attccaagtt ggtttggtaa tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa
    10261 attccaagta atatttaatg ttcctggatt aaccatgttt ttatgccatc tgttagatgt
    10321 ctgttcatct aaaggctcaa agccgctgac tcctgcacta gcaatttttc catcaatgaa
    10381 tgtatttttg gtagcttcga tactttgcgg ttcccattgt gctcttccga cattttgatt
    10441 taagtttcct gctgctgatg ttcctaggta tgcacggctt cctggagaag taatataacc
    10501 atgcgcaaaa cctggaatgc taaaaccgaa caaccctaaa ctaactaacc caataaacaa
    10561 taacgtacta atctttttca tgtagaaacc tccttcattt gataaccaca ttgtaaacgc
    10621 tttattgtat taacacaaac attatgcttt tataatttta tgttgaagta aaaaaggtga
    10681 aaaaagtaag atgcagatta taacagaagt agacgagaaa agtacaaata gaaaacatga
    10741 gaatcaaatc atatgatctt tttcaaaaac atcattcata taaacaaatg ttcaactttt
    10801 taataacaac aattatcaat ttttttctta cttcacaaac taaatacagg tcttgctgtg
    10861 agccttcatg gtaaactaat attatagaaa ggagttagta aaatgaagat gataaaagat
    10921 attcgttttt gggtttgtgt tataggaatt gtcatattag gctttttatc agggttgctt
    10981 agcggaaatc ccggaaaata ttactattct ttgcaacttc caccatttgc accacctagc
    11041 tggatatttg ggccgatgtg gaccttactg tatatattga tgggtatttc tctttatttg
    11101 ttacttaatc acaacaacaa aaaacagcga aacaatcttg tcggtctttt tgtgattcag
    11161 ttcattttta attttatatg gtctgctcta ttttttaatt taagaaatat tttcattgca
    11221 gcaatcgata ttacactttt agttattttc ttgtcagtgc ttatgtatca gttgtggctg
    11281 catcatcgcc tggcaatgtg gctcatgatt ccttattatt tatgggtact attcgcaact
    11341 ctattgaatt attcaattta ttttttaaac taagcgctaa aaagagacgt cctgtaattt
    11401 aacacataga aaaagtgcca cctaagttag
//
LOCUS       DAZULG010000086        11269 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000086 DAZULG010000000
VERSION     DAZULG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.088"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1076)
                     /locus_tag="ACG9CY_002094"
     CDS             complement(33..1076)
                     /locus_tag="ACG9CY_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4938595.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="ACG9CY_002095"
     CDS             complement(1237..1920)
                     /locus_tag="ACG9CY_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFS4938596.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="ACG9CY_002096"
     CDS             2115..3044
                     /locus_tag="ACG9CY_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4938597.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="ACG9CY_002097"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="ACG9CY_002097"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFS4938598.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="ACG9CY_002098"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="ACG9CY_002098"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS4938599.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="ACG9CY_002099"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="ACG9CY_002099"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS4938600.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="ACG9CY_002100"
     CDS             5429..6232
                     /locus_tag="ACG9CY_002100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4938601.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="ACG9CY_002101"
     CDS             6316..7863
                     /locus_tag="ACG9CY_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFS4938602.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="ACG9CY_002102"
     CDS             complement(8160..8357)
                     /locus_tag="ACG9CY_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938603.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="ACG9CY_002103"
     CDS             complement(8433..8633)
                     /locus_tag="ACG9CY_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4938604.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="ACG9CY_002104"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="ACG9CY_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..11111)
                     /locus_tag="ACG9CY_002105"
     CDS             complement(join(9985..10883,10883..11111))
                     /locus_tag="ACG9CY_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4938605.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT         3281 a         2204 c         2245 g         3539 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta ataaccgaca
    10801 taacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa gcgaatttag
    10861 gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc atttaaacga
    10921 gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga ctgatcaggt
    10981 aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc tttcgctaac
    11041 cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa attttcttca
    11101 tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt gtctctacga
    11161 aaagctgtct aagatttgat actaacacca agttatcttt agaaccattt cagatacata
    11221 tgaaacaatt acatatgatt ttttcataaa aaaacagtac tgtctagta
//
LOCUS       DAZULG010000087        11203 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000087 DAZULG010000000
VERSION     DAZULG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.089"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(191..571)
                     /gene="yajC"
                     /locus_tag="ACG9CY_002106"
     CDS             complement(191..571)
                     /gene="yajC"
                     /locus_tag="ACG9CY_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS4938606.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(668..1813)
                     /gene="tgt"
                     /locus_tag="ACG9CY_002107"
     CDS             complement(668..1813)
                     /gene="tgt"
                     /locus_tag="ACG9CY_002107"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS4938607.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            2031..2729
                     /locus_tag="ACG9CY_002108"
     CDS             2031..2729
                     /locus_tag="ACG9CY_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4938608.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2897..5536)
                     /locus_tag="ACG9CY_002109"
     CDS             complement(2897..5536)
                     /locus_tag="ACG9CY_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4938609.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6155..7186)
                     /gene="queA"
                     /locus_tag="ACG9CY_002110"
     CDS             complement(6155..7186)
                     /gene="queA"
                     /locus_tag="ACG9CY_002110"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS4938610.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7379..8554
                     /locus_tag="ACG9CY_002111"
     CDS             7379..8554
                     /locus_tag="ACG9CY_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4938611.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8558..9199
                     /locus_tag="ACG9CY_002112"
     CDS             8558..9199
                     /locus_tag="ACG9CY_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4938612.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9196..10113
                     /locus_tag="ACG9CY_002113"
     CDS             9196..10113
                     /locus_tag="ACG9CY_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4938613.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10110..10781
                     /locus_tag="ACG9CY_002114"
     CDS             10110..10781
                     /locus_tag="ACG9CY_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4938614.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
BASE COUNT         3176 a         2386 c         2136 g         3505 t
ORIGIN      
        1 atataaaatt gctttattta caggtgtttg tatagaaaaa tatataaagt tatttattat
       61 tatttatatg acacacccga ttattcaata ttgagcaata tatcccatgc tcttatctct
      121 ttcttttttg aataaaaaat gaggtaggac atctttctgc cctacctcgt ctttaaataa
      181 gctttgatac ttatttttct tccttctctt ctgtctcttc cagcgtttct gttgtttcag
      241 tttttacttc aactgtctca gctggttctt ctgttgtttc aacagtagtc gttgcatcat
      301 ttgttacgac agtccctggt ttgaccgttt tgattgctgc acgatcgtac tcaaggaaga
      361 tcccttcgca gtctaaaaca actgtccgtt tgtcattgtc gatttctgaa acaactccat
      421 gtaatccgcc gatcgttacg actgaatcac ctggtttcat gctatctaac aagttttgac
      481 gttcttgctg ttgtttcttt tgcgaacgtg acataaaaaa ccacattgct aatagcgcga
      541 taaacatcac aagcattggt aatgttccca tttttttcac ctcactaatt ctgtatctac
      601 aaaaaacact ttagcagaaa aatgctgata acactagttt tttacttaat ttttaaaaga
      661 ttttccttta gaagcttttt gcgttttctt tattgaaccc gtattcttca aaaaaggctt
      721 ctctaaattc aagcaaatta tcatccatga ttgcttgacg tacttgtttc attagattca
      781 acaagaagta taaattgtgg taagaagtca aacgaatccc aaatgtttca tcacatttga
      841 tcaaatggcg gatataggca cgtgtatagt ttttacatgt ataacaatca catttctcat
      901 caattggacg gaaatcatgg gcatatttcg catttttcac tactagacgg ccttgagaag
      961 tcatacatgt gccatttcga gcgattcgag taggcaagac acagtcaaac atgtctactc
     1021 cacggataac accatcgatc aatgaatcag cagcaccgac acccatcaag taacgtggtt
     1081 tgttttcggg gatcaacggt gtagtgaatt ctaatacacg attcatttct gatttaggtt
     1141 ccccgacaga taacccgccg attgagtaac cagggaaatc catcccgact agatctttcg
     1201 cgctttgacg acgaagatct tcaaaccctg ctccttggat gattccaaat aatccttgac
     1261 ggtctggatt agcatgagct ttcaatcctc gttctgccca tcgagacgta cgttctactg
     1321 attttttcac gtagtcgtaa ctttcatcga atggtggaca ttcgtcaaaa ctcatcatga
     1381 tatcagagcc caatttattt tggatgttga ttgctttttc tggtgataaa aacatacgcg
     1441 aaccgttcaa gtgatttttg aaatgcacgc cttcttcttc gatattccgc atatcactta
     1501 atgaaaaaac ttgaaagccg cctgaatctg ttaaaatcgg ttgatcccag ttcataaatt
     1561 tatgcagtcc gccagcttct tctactaagt cttcaccagg gcgtagccat aaatgatatg
     1621 tattacttaa aataacgcca gcacccattt ctttcaactc ttctggggac attgttttta
     1681 ctgtagctaa tgttcctact ggcatgaaca ttggtgtggg gaaagttccg tgtggcgtga
     1741 tcaattcccc taaacgcgcg ccagtatgtt tttctttctt gattaaacga tagcgaatgg
     1801 caggttgcgt cattcatttc cctactttct atataaaata tcttgacaac tcccaccatc
     1861 ataatcgttt caagtgcttt ttgcaagttc cctcggcttc cttctacttt atttaatatt
     1921 ctttttttta tccttttttt atgaataaca aaaaaaagaa aaacattatg acagaaatat
     1981 tttttgatac atggtatatt ggtgttatta aagaaaagga gggaaaaatc atgcaatctt
     2041 ctgcagaatt aaaacgccaa gcaaaagaga gtctaaaagg acgttgggga caagctgttt
     2101 tactaaatct aatccctaca ttgattggta ttgctactat tcttcttatt atgattccta
     2161 ttatcttgtt tgccaccacc atatatgatg gatcagcgag cgtctcagaa gcgacaactt
     2221 caggaggcgg tagttcagga ggcggtggcg gaattatttc cagcatcatt agcgctttgt
     2281 tcatgagcgg tatttcttgg acttatcttg atattttacg tggacaaaaa gatcagatcg
     2341 aaccattcaa agatgcattt cgtggattca aaggtttgtt tatcggcgga gtgatcagct
     2401 tagctgtgct gatctcgatc tttacaacat tatggggttt gctattagtt attcctggaa
     2461 tcattaaaag ctattcttat tctcaaagct attttattta ctacgatatc gtccaagaaa
     2521 caggacaaac acctaaagta ctggatacga tcactgcaag tcgaaaattg atggatggtt
     2581 ataaaggaca acttttctgg ttagatcttt catttatcgg ctggcatatc ttagcattag
     2641 caactgctgg tattggttat ctatggctca gcccatacat ttctgcaaca aaagcagcat
     2701 tttatgaaca attgccaaaa gatatttaaa agagagtgtg acttgtgacc gagggggtgg
     2761 caggagaaca ccgtttatcg agaggcagta aaagaaacat tcagcccatt agcaaaaagg
     2821 aggtgtgaca acattcatgt cacacctcct ttttgtatag ttccagtaaa aagaaaatca
     2881 acttagaaac agttaatcat tcctcttctt ctgcttccgg taatcgatgg aacttgatca
     2941 gttcattcac cgcgattggt acgatagata aaatccctac caagaaccag tgattcaaac
     3001 tgatgtgtac taggccgaat agttcttgcg taaatggcag caaagcaaca gccgttgtaa
     3061 tagctaatgc cagtaacgcc atttctacca aagctttatt ggtcgctaaa ttgactttga
     3121 agatcgattg gctgctgcgt acattgaaca catgcagaat cgatgaataa gctaagatca
     3181 agaatgcaac agtttgaccg acatgctgac ttgcgtctac ccatgggctg atagtatcga
     3241 cataactacc taaataataa ccaaacaagg ttacgatggt gaagacccct gcatttatgg
     3301 caattttttg caataaacct cgagcaaaga tcccttcatt tttcggaatg ggtggctgat
     3361 ccatgatgcc gtgttccgct ggctctcttc ctaaagcaaa tcctggaagt ccatcagcaa
     3421 ctacattgac aaacagtagt tgcacagctg taaatggtgc tccccatcct aacagcatcg
     3481 caatcaaaac aataaagatc tccgaaatat tacagcttag caaaaagttg atagctttac
     3541 gaatattctg ataaaccgaa cgtccttgac tgacagcgtc tacgatcgtc gcaaagttgt
     3601 catccgtcaa aatcatatct gcggcacttt gcgccacatc tgttcctgtg atccccatgg
     3661 cacacccaac gttacttgct ttcaacgctg gtgcatcatt cacaccatcc ccagtcatcg
     3721 ctacgactgc acccgtacgt tgccaagatt tgacgatccg gatcttgtct tctggcgtta
     3781 cccgagcata gacagagaga gctttgacac gcgcatctaa ttcttcgtct gataattgat
     3841 gcagttctga tcctgaaagt gcttctgacg gatcattcaa gatacctaat tctttcgcta
     3901 tggcactggc agtcaccaca tgatctccag tgatcatgac tgttttgatg ccagcctttt
     3961 tcgctcgctt gattgctcct ttactttctg gacgtggtgg atcgatcatt ccgatcagac
     4021 caatcaaacg taagtctttt tccagtgctt cagaagaaat atcttgcggt tcttcgtcat
     4081 aagtagcata accaacagca atcactctca gtgcccgttt accaaagcga tcattaacga
     4141 tcgccgcttg ttctacatcg cctgaactaa agcgcggagc aagaacatcg aatgctcctt
     4201 tggtgattga gatgtatttt tcgcccattt ggtgaactgt tgtcatcatt tttcgttcag
     4261 aatcaaaagg aagctcgttt actcgtggat atttttcttc taattctgcc tttgtatgat
     4321 aattctcttc aacagccctt acgatggcta cttctgttgg attacctttg ataacaaggt
     4381 cgtcatcttc ttgctcgacc gtcacatctg tacatagtgc agccattttc aatatttcca
     4441 tcgcgctgtt cgtcattgaa tcttcgatat tcgttacttc atcttctttg ctccataccc
     4501 ggcggacgcg catttcattt tgtgtcagcg ttcctgtttt gtctgagcag ataacattag
     4561 cggttcctag cgtttcaact gctggcaact gtcggatgat cgcatgtttc ctcgccatct
     4621 tctgcacacc gtatgcaagt gtcaacgtaa caataacagt caaggtttcc ggtacagctg
     4681 caacagccaa ggaaatcgaa gtcatgaaca tctcaaggag tggttctcct tgcagttcac
     4741 caatcacaaa tactaaagcc gcagccgcta aagcgatcat actgattcgt ttgcctagct
     4801 gattcaatcg gacctgtaaa ggtgtttttt gcatagtatt ttcgttcaaa agatccgcga
     4861 tcttacccat ttctgtagcc atgccaatgg cagtgacgat cgctttccca cgacctgcag
     4921 caaccgtaca tcctttaaat accatattaa gttggtcacc taatgactca tcttcttcag
     4981 caataaatgc tgcatctttt tcaactgctt cactttctcc agtcaacgcc gattcttcta
     5041 cacgtaattg tgatgcttcg atcaaacggg catcggcaga aatagcatca ccggactcta
     5101 aaacaagaat atcacccact accagttgct ctgactcaac agtggtcact ttgccgtctc
     5161 gtatgacagt tgccgtttgt ttattcatat cttgcaagga ttccagtgct ttttctgcgt
     5221 ttccttcttg gacaatcgcc aataccgagt tgatcacaac gatagcgatg atcagcagtc
     5281 cttcaaataa atccccatgt tctgtagcaa atgcggtata aaaggaaatc accgctgcta
     5341 caagaaggat gatcgttgta aaatcggaca agcttcgaaa gaattttttg atcatcgatt
     5401 ctttcggcgc ctcatcgaat ttattcaatc cctgcttttt tcgccgttca tccacttctt
     5461 gtgaagacaa cccagtatct ggatctgctt ctgttacctt ctttacttct tctattgtct
     5521 gcttgtattc ttccatccta ttcctccttt aaaatcttcg tacaaaaaaa ggtacttatt
     5581 ttactataac atcctttttt tatttacttc aaataaaggg cttatcgatt atttgttaag
     5641 aaagcgtaaa atttaaagtg cttatatttg caaacgctac caaaggatgc tattatatag
     5701 atgtaaggaa ggaggatacc atcaaataga cagaagaatt tctcgataga acagtgtaag
     5761 caaacaaagt tgttagaagt acaggtttca cccagacatg cgtgtagagt acaaacctat
     5821 aaatagtgaa tgcgtatgga tgtttggatc acgaagtatc cgaaacaaga agtaatgcga
     5881 tacgtaacaa ttgaataacg taaacgtctg aaaattgaat aggaacaaga gatgtttcaa
     5941 atgttgaaga tgtaacgaaa acaaaatttt tgtttaacat gaagaaattc aacaccgtgc
     6001 tttgcatgta tgatggaaag ttacaatcat ttctgtttat ttgaatcctt ccgaggtaca
     6061 agaaagccga gcttatcgag agctcggctt tttctttgtc ataaaatctt tgtaaagctt
     6121 ttttgttatt caaaactagc ggcttttcat aaccttattt cacgaacatc gcatctccaa
     6181 aactaaagaa acgatagcgc tcttcaatcg catgctggta agcagataaa gtcaattctc
     6241 ttcctgcaaa ggcactaact aacatgacta aagtcgattt tggcaaatgg aaattagttg
     6301 agaaagcttc taccactttg aattcatagc caggcgtaat gaaaatatca gtccatccgc
     6361 tgtctgcttt gatttctcca ttaaatttcg ttccaatcgt ttctaatgtt cgaattgaag
     6421 ttgttccgac agccacgatt ttcccgccat ttttacgtac ttggttcaat cgctcagccg
     6481 cttcttccgt caaacggtag aattcactat gcatatggtg ttcttcaatt ttatctacgc
     6541 tgacaggtcg gaatgtacct aaccctacat gtagtgtcag atagactaat tcaacaccct
     6601 ttgcttcgat ctcatcaagt aattctttcg taaaatgcaa tccagctgtt ggcgctgcag
     6661 cagaaccatt ttctttcgca tagacagttt gatagcgatc tgggtcttcc agacgttctt
     6721 tgatatatgg aggcaatggc atttctccca atgattcaag aatctctaga aaaattcctt
     6781 catactcaaa gtcaatgatc cgtccgccgt gttccaattc ttctttcact actgcttgca
     6841 atctgccatc accaaaagag attcttgtcc caactttggc acgtttcgca ggtttgatca
     6901 atgtctccca cgtatctcct tgtgtattcg ttagaagaag gacttctaaa tgcgcaccag
     6961 tatctggttt ttctccatat aaacgagcag gcagaactcg tgtattattc ataactagcg
     7021 catcaccagg ctgtaactct tcaatgattt catggaaatg tttatcttcc atctctccag
     7081 ttttccgatc caaaacaagt aaacgagaat gatcgcggtc ttttaatggt gtttgtgcga
     7141 ttaattcttc cggcaagtca aaatcaaaat cttctgttgt taacatatcg tcactcactt
     7201 tctatcatgt gcttcatcat tctatcatgt ttttcgttct gttggcaaaa tcagcacttt
     7261 ttcacttcta ttttttgttt tcctaaataa aaaaactacc ctatttgtat tttttcattg
     7321 ttttaggcat actagtaata gatagttctt cattgaaaat agaaaaagag gtggcaccat
     7381 gattgaattt caaaatgtgt caaaaattta taaaggtgga aaagtcgctg tagaggacgt
     7441 caatctttca tttgaaaagg gtgagttcat ttgttttatc ggtacgagcg gaagcggaaa
     7501 aacgacgacg atgcggatga tcaaccgaat gactgatcca acacaaggga aaattttgat
     7561 tgatggaaaa tccatccaag aaattgatcc agtggaattg cgacgtcaga tcggctatgt
     7621 tatccaaagt attggattga tgcctcacat gacgatccgt gaaaatatca ctcttgtcca
     7681 aaaattgttg aaagtcagtc aagaagaacg gaataaaaca gctgaaaaaa tgattgatct
     7741 tgtggaattg ccccgtgaga tgttagatcg ctacccacat gaattatctg gtggtcagca
     7801 gcagcgtatt ggagtcgttc gtgcccttgc agcgaatcaa gatatcatct tgatggacga
     7861 gccattcggt gcgcttgatc caatcactcg tgattccttg caagaccttg tcaaagattt
     7921 acaagagcgt ttgggaaaaa cgatcgtatt tgtaacccat gacatggatg aagcgattaa
     7981 actagcaagc cgtatcgcca tcatgagtga aggccgtgtg atccaatttg atacaccaga
     8041 aaatatttta cgtcatcctg caaacgattt cgtagaagag ttgattggag aagatcgcct
     8101 actacaagcc aagccaaatg cgacaagagt cggcgaagtt atgctaaata cagcaatcac
     8161 gattacaccg gaaaaatcat tacaagaagc aatcaaactg atgagggaaa aacgtgtaga
     8221 tacgttgtta gttaccgata attcgaatgt attaaaagga tttatcgatg tagaaacgtt
     8281 gaataaaaaa cgaggaaaag tatccagtgt cggcgacatt ttgaataaac atgtttttta
     8341 cgtaaaagaa tccgcctatc ttcgtgatac cttgcaacgc attttaaaac gcgggctgaa
     8401 atacgtgccg gtcgttgatg aacagaacaa agtcgtaggt atcctgactc gtgcatcatt
     8461 agtggatatc gtctacgatg tgatttgggg agaagatccc gcaactactg atgttgcaga
     8521 gtcagctact cctgaagaat ctaaggaggt ctgacctatg cagcagtttt ttcatcagca
     8581 cggttcagaa attctctcaa aaggttttga acatctttat atttcaggac ttgctttgat
     8641 cattggtatc atcattgctg taccgcttgg tgtcttactg acacgttttc caaaagctgc
     8701 cacgattgtg attggcttaa ccagtgcctt gcagacaatc ccttctcttg ctttattggc
     8761 attgatgatc ccgctatttg gcgtaggtaa attacctgcg attatcgcac tctttattta
     8821 ttcattattg ccgattttac gtaatacgta cattgggatg aaaaatgtcg attggaatta
     8881 tcgggatgtg gcaaaaggca tggggatgac tgagttccaa tccatttttt ccgttgaatt
     8941 gcccattgct atgccaacga tcatggctgg tattcgttta gctgccgttt acgtgattgc
     9001 atgggctaca cttgcctctt atatcggcgc tggtggttta ggcgatttga tttttagtgg
     9061 actgaataat taccaaccag atctcatttt ggcaggtacg atccctgtaa caattttagc
     9121 actattggct gattggcttc taggtctctt ggaacaccgg ctgacaccga tcgcacttag
     9181 agaggagaat gaagaatgaa acgtatacta aaaatgacca tggttctttt ctctcttctt
     9241 atacttgcga gttgttcctt ccctggtctt gccagcaata cagatgaaga caccatttcg
     9301 atcacaggtg gaatcacttc agaagcacag atcttagcaa gtctagttgc cggaatggtt
     9361 gaacattata cagataaaaa taccgcaatc atcaacaatc tggctactac aactatcaac
     9421 catcaagcga tgatgaacgg agatgcttca atttcagcgg ctcgctacac aggaacagat
     9481 ttaacgacta cattgaacct tcctcctgaa aaagatccga aaaaagcgtt tgctaccgtg
     9541 aaagatgaat ttgagaaaag atacggtcaa acgtggttcc cgtcctatag ttttgaaaat
     9601 acgtatgtct ttcttgttcg aaaagacacc gcacaaaaat accatctatc taaagtcagc
     9661 gatctgaaaa atgtagcgga tgaattagta gctggtgtag atacttcttg gatcaatcgt
     9721 aaaggagacg gttatgacgg cttccaagaa acttatggct tctccttcaa ttcgatatta
     9781 cctatgcaga ttggacttgt ttatgacgca gtcgaagcag gaaaaatgga tattgttctt
     9841 ggctactcta cggatggacg gatcgccagt tatgatctag tcatgttgga agatgatcgt
     9901 caattcttcc caccatatga tgctgctcca gttgtagaca acaagatatt agctgatacg
     9961 cctcatttaa aagaagcctt aaataagtta ggtcatacca tcagtacgga aaaaatgcag
    10021 gaattgaact acgaagcaga taataatcta gtggaaccat cagtagtagc acaacgtttc
    10081 ttacaagaaa accactattt tgaagggaag tgaagcaatt ggataaaatg aatgtctttc
    10141 aacaattttt ctattatttc caggaaaatg gctcctatat ctttgcacaa tttatccgtc
    10201 acttcctgat ttctatctat ggtgtcctct ttgcagcagt tgtcgggatt ccggttggga
    10261 tcatgatttc taggagacgt aaattagcaa actgggtgat tcgtttggcg aatatcatcc
    10321 agacgatccc ttctctagcc atgatctcca ttttaatcat tggattaggc ctgggagtca
    10381 atgtcgtgat tgttactgta tttttgtatt ccctcttacc gatcatcaag aatacgtata
    10441 ccgggatgat ccaagtagac aaaaacatcc ttgatgtcgg caaaggcatg gggatgactg
    10501 ctagacaacg actattcatg gtagagttac cactctctgt ttctgttatc atggcaggca
    10561 taagaaacgc gttagttgtt gccatcggga taaccgcgat cggtgcattc gtcggtgctg
    10621 gtggtcttgg agatatcatt atccgaggaa ccaatgcaac agagggcaca tcgattattt
    10681 tagcaggtgc attgccaact gcattaatgg cgatcatcac ggactggctt ttaggtattt
    10741 tggaaagacg gctagatcca gccagccgta catcacgctg atgttattgt aaaaaacaaa
    10801 agcttctgcg accatttgtt ggtcgcaaag gcttttttag ttctgtcatt tttaaggcaa
    10861 taatccactc tgattcttcc ctccttatgg gcaaagagtt gcttttctta gctatttatc
    10921 cattccgtat actccaactt catactgcca acgggttcat atctgcatcc accgactaag
    10981 tttttttctt atccgctaag gcattttgcc aatccatttt cccatcactc gtcatggaat
    11041 tcataccaca tgatctctaa atactcgtct gcttgttctt tgaattgggc atcaattctt
    11101 acatcctttg gcgtggcttt catttaagcc ttttccatca gtcctcgatt gttcgatgcc
    11161 ttcagctgca tcatcaaaac tggtgactgc cgtatcggta act
//
LOCUS       DAZULG010000088        11201 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000088 DAZULG010000000
VERSION     DAZULG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.090"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG9CY_002115"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG9CY_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS4938615.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG9CY_002116"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG9CY_002116"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS4938616.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACG9CY_002117"
     CDS             complement(2169..2906)
                     /locus_tag="ACG9CY_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4938617.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACG9CY_002118"
     CDS             complement(2997..3524)
                     /locus_tag="ACG9CY_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938618.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACG9CY_002119"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACG9CY_002119"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS4938619.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACG9CY_002120"
     CDS             complement(4401..4886)
                     /locus_tag="ACG9CY_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS4938620.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACG9CY_002121"
     CDS             complement(5039..5236)
                     /locus_tag="ACG9CY_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938621.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACG9CY_002122"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACG9CY_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS4938622.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACG9CY_002123"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACG9CY_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS4938623.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="ACG9CY_002124"
     CDS             9211..9423
                     /locus_tag="ACG9CY_002124"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS4938624.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="ACG9CY_002125"
     CDS             9425..11104
                     /locus_tag="ACG9CY_002125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4938625.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3248 a         2420 c         1966 g         3567 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac a
//
LOCUS       DAZULG010000089        11118 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000089 DAZULG010000000
VERSION     DAZULG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.091"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..1339
                     /gene="lysA"
                     /locus_tag="ACG9CY_002126"
     CDS             80..1339
                     /gene="lysA"
                     /locus_tag="ACG9CY_002126"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS4938626.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1326..2126
                     /gene="dapF"
                     /locus_tag="ACG9CY_002127"
     CDS             1326..2126
                     /gene="dapF"
                     /locus_tag="ACG9CY_002127"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS4938627.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2129..3010
                     /gene="dapA"
                     /locus_tag="ACG9CY_002128"
     CDS             2129..3010
                     /gene="dapA"
                     /locus_tag="ACG9CY_002128"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS4938628.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3007..3723
                     /gene="dapB"
                     /locus_tag="ACG9CY_002129"
     CDS             3007..3723
                     /gene="dapB"
                     /locus_tag="ACG9CY_002129"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS4938629.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3729..4424
                     /gene="dapD"
                     /locus_tag="ACG9CY_002130"
     CDS             3729..4424
                     /gene="dapD"
                     /locus_tag="ACG9CY_002130"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS4938630.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4440..5441
                     /locus_tag="ACG9CY_002131"
     CDS             4440..5441
                     /locus_tag="ACG9CY_002131"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS4938631.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5438..6799
                     /locus_tag="ACG9CY_002132"
     CDS             5438..6799
                     /locus_tag="ACG9CY_002132"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS4938632.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6759..7910
                     /locus_tag="ACG9CY_002133"
     CDS             6759..7910
                     /locus_tag="ACG9CY_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4938633.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7885..9054
                     /locus_tag="ACG9CY_002134"
     CDS             7885..9054
                     /locus_tag="ACG9CY_002134"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4938634.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9229..10608
                     /locus_tag="ACG9CY_002135"
     CDS             9229..10608
                     /locus_tag="ACG9CY_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4938635.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10889..11118
                     /locus_tag="ACG9CY_002136"
                     /pseudo
     CDS             10889..11118
                     /locus_tag="ACG9CY_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3700 a         1945 c         2493 g         2980 t
ORIGIN      
        1 attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac ggaaaaaaag
       61 tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag caagcacatt
      121 ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga gacaaacgat
      181 gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc tttacgcctc
      241 taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat tcgggctaga
      301 tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta gagatactat
      361 ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg aaaatgattt
      421 gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat taagccaacg
      481 ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag cacatactca
      541 tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg aaagtaaaga
      601 gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag aaggatttca
      661 tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa tcgacaaatt
      721 gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg gagggtttgg
      781 gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa aaaaactgat
      841 tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat tactgatcga
      901 accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg gtttcatcaa
      961 aaagacacca cataaaaaat attactttgt agatggcggg atgacagata atatccgtcc
     1021 agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg aagaaaaaac
     1081 agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa ttaaagaagc
     1141 ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg gagcttatgg
     1201 atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt ttgtcaataa
     1261 tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa ggggggaagt
     1321 ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta ttagattacg
     1381 cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa aaatatgata
     1441 tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac aacaaagatg
     1501 gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac gtaaatgatc
     1561 agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca agaaaaatta
     1621 cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca gatgattctc
     1681 cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata gagatcaatg
     1741 gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt gtttttgttg
     1801 aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat catcctttgt
     1861 tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa ttgatcgtcc
     1921 gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc tgtgcctctt
     1981 atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt cacttggaac
     2041 aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca gcagttcatg
     2101 aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg cgctgatcac
     2161 gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga tccaattcca
     2221 tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat cttctacgct
     2281 cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg ctggccgtgt
     2341 gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa aagcgaagca
     2401 ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca acaaaacaag
     2461 tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga ttccgcttat
     2521 tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat tagtaaattt
     2581 agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg cttatgtgat
     2641 ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg atgatttgat
     2701 cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga atatccaacc
     2761 taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc aagcaaaaga
     2821 aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca atcctattcc
     2881 tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc tgcctttagt
     2941 ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag aaaggacggt
     3001 tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt cgaagaagtt
     3061 gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga agcaactacg
     3121 cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga taatttagag
     3181 aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac gacaggttat
     3241 acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt gatgtatagt
     3301 gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc agctgcaatg
     3361 ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa agatgcacca
     3421 tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct gcagcccgta
     3481 tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag tattcgtgca
     3541 ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat atttacgatc
     3601 aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc agcggactgg
     3661 ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga caaaggggtg
     3721 taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct aaaaagacgc
     3781 ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt aaagtttttg
     3841 gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg gttttagaag
     3901 ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac agcgcagttc
     3961 ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt atcagagatc
     4021 aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat attggtgcag
     4081 taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga gcgacagtgg
     4141 gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa ccagcatcgg
     4201 ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg attgttgaag
     4261 gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta gaagatgtgg
     4321 cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt gatgatcaga
     4381 caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga tggagaaaaa
     4441 tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa acgatgctca
     4501 aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct tcaaaacgtt
     4561 cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa ttgacagaaa
     4621 cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta tccaaagagt
     4681 ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca gcatggcggg
     4741 aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat acattgaatc
     4801 ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt aaagctttac
     4861 aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt tctggttccg
     4921 ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca gttttgtatc
     4981 cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa aatgggtata
     5041 caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag cctgctttgc
     5101 cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc tctgtggcag
     5161 ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc cagtttccag
     5221 ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt cttgctagag
     5281 gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag aacggattgt
     5341 tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc gtacagattg
     5401 ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc tgaaagtagc
     5461 gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc gtacaattgt
     5521 tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac gtacgtcaga
     5581 tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc atcatgttga
     5641 ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag atgttcttgc
     5701 cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg aagacgaaaa
     5761 gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac tgatggctga
     5821 gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact atgatgggaa
     5881 aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat atccgaaaat
     5941 ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat tgggccgtgg
     6001 gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa gttatgagaa
     6061 ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc atcctaaaat
     6121 catcgaggaa atctctttta gagaactgag agagatggct tatatggggg ctaacgttct
     6181 tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa taaaaaatac
     6241 caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa aggaaaatgg
     6301 actgacagga atagctggac gcaaggattt tctttccatc actttattca aacgtcatat
     6361 gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac atgagatatc
     6421 aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag cagaagcgat
     6481 tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg tggaagaaat
     6541 cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata aagaattgat
     6601 tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca catcgatctt
     6661 atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc aatatgaaac
     6721 agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa tatcgaatgg
     6781 cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa tatttatatg
     6841 aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga ctactccttt
     6901 attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat gcactgccag
     6961 tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg catgcatgtg
     7021 gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact aaacacattg
     7081 aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt tccggtggcg
     7141 cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca atatttggct
     7201 ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga cctttgatgg
     7261 cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt gcttctagtg
     7321 aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt tatgattttg
     7381 accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc caaggtggaa
     7441 cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt tcttacagtt
     7501 cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag tttcactcta
     7561 cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac catagaggct
     7621 tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca gttcttcaag
     7681 ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt ttaggtgtcg
     7741 gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt ttagaaaatg
     7801 gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa atcgatagca
     7861 gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag tcctaggctt
     7921 tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc gataccagat
     7981 gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt caagcaagca
     8041 ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg attagaggaa
     8101 ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta tgatccgcaa
     8161 acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt gttatctgtt
     8221 ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata tcagccatta
     8281 gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg gtttgtctgt
     8341 cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa agcagtcatt
     8401 ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt gaaagcaatc
     8461 gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata tgctgaattg
     8521 acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac aatcgtgata
     8581 agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat ttttgctcaa
     8641 aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc cgctacaacg
     8701 atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag cgagaatatg
     8761 aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc tttaggattt
     8821 gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc tgccattcaa
     8881 actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc gtgtatccca
     8941 ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc tagcagcatg
     9001 gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca gtgattcata
     9061 gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag acgattgttt
     9121 tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta atgttaagtt
     9181 taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat ggaaaaaatc
     9241 aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga actattgaca
     9301 cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat ttccttcatg
     9361 tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga tgtacgtaat
     9421 ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca cgaagtaaaa
     9481 acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa cgaaacaaaa
     9541 gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa tctgccagta
     9601 ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc aaccaaaatc
     9661 aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc gggttacatt
     9721 gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt gcttgatgtg
     9781 atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt agaacaacac
     9841 ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt tactggtaac
     9901 ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct agtcattcaa
     9961 gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt agatgaacgt
    10021 ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta tgcagtagga
    10081 gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc tttagcgact
    10141 gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt accaaacaaa
    10201 ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca ttttgcagca
    10261 agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt gtcttattat
    10321 gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa agtgtccatc
    10381 caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt atctacctat
    10441 gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact gactttagaa
    10501 gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg gagcatgttg
    10561 aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga aaacgaaata
    10621 tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag ccaagtaaaa
    10681 acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca
    10741 ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc aaaagttaga
    10801 ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact tttgaactta
    10861 aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta aaaatccaga
    10921 tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt atggctgttt
    10981 acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga aagttcagcg
    11041 cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac gtcgctacaa
    11101 ttcttatcga ggaaaagt
//
LOCUS       DAZULG010000090        10565 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000090 DAZULG010000000
VERSION     DAZULG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.092"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG9CY_002137"
     CDS             33..656
                     /locus_tag="ACG9CY_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938636.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG9CY_002138"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG9CY_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4938637.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG9CY_002139"
     CDS             2152..3033
                     /locus_tag="ACG9CY_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4938638.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG9CY_002140"
     CDS             3036..3806
                     /locus_tag="ACG9CY_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938639.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG9CY_002141"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG9CY_002141"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS4938640.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG9CY_002142"
     CDS             5117..6280
                     /locus_tag="ACG9CY_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4938641.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG9CY_002143"
     CDS             6397..6807
                     /locus_tag="ACG9CY_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4938642.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG9CY_002144"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG9CY_002144"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4938643.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG9CY_002145"
     CDS             9380..9664
                     /locus_tag="ACG9CY_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938644.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG9CY_002146"
     CDS             9824..10060
                     /locus_tag="ACG9CY_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4938645.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG9CY_002147"
     CDS             10057..10455
                     /locus_tag="ACG9CY_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4938646.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3688 a         1770 c         2232 g         2875 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaata
//
LOCUS       DAZULG010000091        10483 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000091 DAZULG010000000
VERSION     DAZULG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.093"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(222..707)
                     /locus_tag="ACG9CY_002148"
     CDS             complement(222..707)
                     /locus_tag="ACG9CY_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS4938647.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    797..1142
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1187..1828
                     /locus_tag="ACG9CY_002149"
     CDS             1187..1828
                     /locus_tag="ACG9CY_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4938648.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1847..2482
                     /locus_tag="ACG9CY_002150"
     CDS             1847..2482
                     /locus_tag="ACG9CY_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4938649.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2500..3315
                     /locus_tag="ACG9CY_002151"
     CDS             2500..3315
                     /locus_tag="ACG9CY_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4938650.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3634..5070
                     /locus_tag="ACG9CY_002152"
     CDS             3634..5070
                     /locus_tag="ACG9CY_002152"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4938651.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5259..7706
                     /locus_tag="ACG9CY_002153"
     CDS             5259..7706
                     /locus_tag="ACG9CY_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4938652.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7936..9003
                     /gene="rlmN"
                     /locus_tag="ACG9CY_002154"
     CDS             7936..9003
                     /gene="rlmN"
                     /locus_tag="ACG9CY_002154"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS4938653.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8993..9199
                     /locus_tag="ACG9CY_002155"
     CDS             8993..9199
                     /locus_tag="ACG9CY_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938654.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9196..10299)
                     /locus_tag="ACG9CY_002156"
     CDS             complement(9196..10299)
                     /locus_tag="ACG9CY_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4938655.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3462 a         1794 c         2282 g         2945 t
ORIGIN      
        1 ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt cgtagaatca
       61 ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact
      121 agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga
      181 aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact
      241 ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta
      301 gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc
      361 acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct
      421 ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt
      481 aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag
      541 atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa
      601 aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc
      661 agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct
      721 ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt
      781 acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag
      841 agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt
      901 tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg
      961 accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta
     1021 acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg
     1081 tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct
     1141 tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat
     1201 tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac
     1261 cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa
     1321 gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt
     1381 gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga
     1441 agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg
     1501 agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc
     1561 ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt
     1621 agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg
     1681 tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct
     1741 aggcgtggca atcatctatc ttacattgac tggtattcta acaatcgtaa tgaaacaagt
     1801 tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa
     1861 gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc
     1921 agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg
     1981 tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt
     2041 caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca
     2101 gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa
     2161 acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag
     2221 tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagcgat
     2281 tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt
     2341 agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt
     2401 aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact
     2461 aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta
     2521 ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca
     2581 gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt
     2641 gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc
     2701 aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg
     2761 aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg
     2821 ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg
     2881 atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt
     2941 gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag
     3001 atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg
     3061 aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa
     3121 caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt
     3181 gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat
     3241 caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt
     3301 acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc
     3361 gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa
     3421 cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat ttatgcaaaa
     3481 atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac
     3541 aacctccttt acataatttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg
     3601 aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt
     3661 ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa
     3721 gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt
     3781 aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct
     3841 ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt
     3901 cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac
     3961 gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa
     4021 gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat
     4081 tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat
     4141 gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca
     4201 gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc
     4261 aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca
     4321 aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg
     4381 atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg
     4441 ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa
     4501 tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat
     4561 ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa
     4621 gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat
     4681 tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt
     4741 taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa
     4801 gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc
     4861 aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat
     4921 ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac
     4981 gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa
     5041 ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct
     5101 ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata
     5161 agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt
     5221 ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta
     5281 ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc
     5341 tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag
     5401 gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa
     5461 tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt
     5521 caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat
     5581 tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga
     5641 catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg
     5701 ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt
     5761 atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat ctggaaggct
     5821 atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg
     5881 atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag
     5941 aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat
     6001 atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac
     6061 cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata
     6121 tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag
     6181 aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag
     6241 cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa
     6301 ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa
     6361 ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca
     6421 caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag
     6481 atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta
     6541 aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa
     6601 tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg
     6661 tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt
     6721 cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg
     6781 ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt
     6841 acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag
     6901 ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag
     6961 taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga
     7021 aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg
     7081 tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg
     7141 ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca
     7201 aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga
     7261 tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa
     7321 tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac
     7381 gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga
     7441 cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag
     7501 caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc
     7561 ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg
     7621 gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa
     7681 cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac
     7741 caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct
     7801 aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat
     7861 ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa
     7921 aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc
     7981 aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg
     8041 tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa
     8101 aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg
     8161 gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg
     8221 atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc
     8281 tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt
     8341 gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt
     8401 tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac
     8461 tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt
     8521 tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat
     8581 ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac
     8641 cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac
     8701 cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg
     8761 caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca
     8821 tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga
     8881 ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca
     8941 gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa
     9001 taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga
     9061 actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac
     9121 aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg
     9181 ggttttagca attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg
     9241 ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac
     9301 atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac
     9361 attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat
     9421 tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac
     9481 agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga
     9541 gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac
     9601 aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt
     9661 caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc
     9721 taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt
     9781 ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc
     9841 tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc
     9901 ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg
     9961 aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg
    10021 gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg
    10081 ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc
    10141 ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat
    10201 cagcactact tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt
    10261 tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg
    10321 ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga
    10381 ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa
    10441 taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cga
//
LOCUS       DAZULG010000092        10390 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000092 DAZULG010000000
VERSION     DAZULG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.094"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG9CY_002157"
     CDS             6..305
                     /locus_tag="ACG9CY_002157"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4938656.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG9CY_002158"
     CDS             378..1739
                     /locus_tag="ACG9CY_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS4938657.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG9CY_002159"
     CDS             1761..2021
                     /locus_tag="ACG9CY_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938658.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG9CY_002160"
     CDS             2035..2787
                     /locus_tag="ACG9CY_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS4938659.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG9CY_002161"
     CDS             complement(2881..3486)
                     /locus_tag="ACG9CY_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4938660.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG9CY_002162"
     CDS             complement(3539..4513)
                     /locus_tag="ACG9CY_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS4938661.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG9CY_002163"
     CDS             complement(4891..5610)
                     /locus_tag="ACG9CY_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4938662.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG9CY_002164"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG9CY_002164"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4938663.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG9CY_002165"
     CDS             7161..8558
                     /locus_tag="ACG9CY_002165"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4938664.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG9CY_002166"
     CDS             8639..9079
                     /locus_tag="ACG9CY_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4938665.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG9CY_002167"
     CDS             9227..9553
                     /locus_tag="ACG9CY_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4938666.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG9CY_002168"
     CDS             9554..10303
                     /locus_tag="ACG9CY_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS4938667.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3293 a         1832 c         2151 g         3114 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat
//
LOCUS       DAZULG010000093        10318 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000093 DAZULG010000000
VERSION     DAZULG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.095"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..904)
                     /locus_tag="ACG9CY_002169"
     CDS             complement(71..904)
                     /locus_tag="ACG9CY_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS4938668.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1208..3373)
                     /locus_tag="ACG9CY_002170"
     CDS             complement(1208..3373)
                     /locus_tag="ACG9CY_002170"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS4938669.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3633..5927
                     /locus_tag="ACG9CY_002171"
     CDS             3633..5927
                     /locus_tag="ACG9CY_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS4938670.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5920..6585
                     /gene="pgmB"
                     /locus_tag="ACG9CY_002172"
     CDS             5920..6585
                     /gene="pgmB"
                     /locus_tag="ACG9CY_002172"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4938671.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6578..7585
                     /locus_tag="ACG9CY_002173"
     CDS             6578..7585
                     /locus_tag="ACG9CY_002173"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4938672.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7607..8626
                     /locus_tag="ACG9CY_002174"
     CDS             7607..8626
                     /locus_tag="ACG9CY_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4938673.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACG9CY_002175"
     CDS             complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACG9CY_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4938674.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         3198 a         2242 c         1909 g         2969 t
ORIGIN      
        1 ctctccaaac ggtataatcg tttggagagt tttattttaa tcagtttttt tatgttatat
       61 gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga tcactgatga
      121 ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt ccttcaggga
      181 cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct attttctttt
      241 ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga agaagaaggc
      301 aactttctct tacagtataa taaccttgag agtctactgg attattgaaa tcacctagga
      361 aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac tcaaaataaa
      421 agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa atgtcctgat
      481 catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct ttcttttgaa
      541 attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt gcatttcctt
      601 cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac tcttttccgt
      661 gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata ggtacttgat
      721 tctttaccgg acagaacttc attagcttcg caggttctac ttcttcagat tccagtaatt
      781 ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta tctgctaatt
      841 cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt gtcatgaatt
      901 tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa atttttgtca
      961 caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct caatggacca
     1021 ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt ttcacgacct
     1081 ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca aacgaaaaat
     1141 aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa
     1201 cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta cctaattgct
     1261 tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt ggatcttttt
     1321 cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt cctacttctt
     1381 ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta tcgattccca
     1441 tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt ttgctaggga
     1501 aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt ggaataacag
     1561 caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca gataaaggaa
     1621 tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat tgcttactac
     1681 ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct cctgattgca
     1741 gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact gcttggactc
     1801 cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct tcagtcccta
     1861 ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg acatctttga
     1921 tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct gctgttcctg
     1981 ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg ttacgtcccg
     2041 gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta acaacaccaa
     2101 aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct ttgatagcta
     2161 acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat ggcaagatat
     2221 ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat ggcacagcag
     2281 caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca gccaacgctg
     2341 ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta cgatacatcg
     2401 caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact ttgaaacgag
     2461 ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca cctttaagat
     2521 tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact tgtgtccccg
     2581 cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg ataggaatcg
     2641 tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca aataaaaacg
     2701 gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa ttattgattc
     2761 cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt gaccataaga
     2821 taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc ggcaattttc
     2881 tataattata gtacttatta taagccattg ccccgacaaa tccagcgatg atccctacga
     2941 atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct ttaaccatga
     3001 tcttcgtacc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg tcacttgtga
     3061 caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca gctgcaaatg
     3121 ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat aaaatatgaa
     3181 gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg acaagcggcg
     3241 tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac ccagctgccg
     3301 gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaac tcaaaactaa
     3361 atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac gtattatagc
     3421 gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac cattctataa
     3481 aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt tttttaagct
     3541 tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc gtttattatg
     3601 aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga ctatttgcta
     3661 ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga ctgcaagaat
     3721 ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa gaaacttact
     3781 caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat aagacacgtg
     3841 tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac gccttgaact
     3901 ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct ttggaaccag
     3961 aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca ttcacaatca
     4021 ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt gtcaaaaaag
     4081 aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca gtcaaactcg
     4141 tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa catttttgga
     4201 atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa actattccaa
     4261 acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc gttaatggaa
     4321 aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc ttgtctgcag
     4381 aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt actagtagag
     4441 atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac cagacaaaca
     4501 attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg aagcttgccg
     4561 atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat ttgttccaac
     4621 tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa ggattcacag
     4681 gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc cctctttatc
     4741 ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat aatcaactgc
     4801 cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat cctatggtca
     4861 ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag attcaccgaa
     4921 atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat acggaatatt
     4981 tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca gaccgtgtcc
     5041 atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca aacgaatatg
     5101 agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc ttgcgttaca
     5161 cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc gaagaagaac
     5221 tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa gaattaggaa
     5281 tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc gatattccca
     5341 caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga tcttgcttca
     5401 tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt acaaaagaag
     5461 aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct tccctttcgc
     5521 caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct gtagaaatgt
     5581 accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa gacggcttgc
     5641 acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct caaatgaaaa
     5701 ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa gcttattctt
     5761 tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa caagttactt
     5821 tcgagttatt agaaggttct cctctagaaa tttacgtcta tgaacagtct tattctttag
     5881 cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg ggtattgttt
     5941 gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg gcgaaagctt
     6001 ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa aggcgtcagc
     6061 cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc tttttcaaag
     6121 gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat gatccagacg
     6181 atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt aaaagaagaa
     6241 aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact tgaaaaaatg
     6301 ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa cggaaaacct
     6361 gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga agaatgtatc
     6421 gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc tcaaccaatt
     6481 ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga tacgactttt
     6541 cttacgatcg attacttgaa agaaaagtgg catgaccatg gataatccag agatctccgt
     6601 cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact acttagaagt
     6661 cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca aagaaggaaa
     6721 atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag acaaaagctt
     6781 ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt ggggtgagca
     6841 tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag gctggtcttt
     6901 ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg tcttttactt
     6961 aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt accgtttaac
     7021 aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca tctgcaatcc
     7081 gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct atgaacatca
     7141 gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc ctactggaaa
     7201 ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac tgcaagagat
     7261 ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc ctaggctttc
     7321 caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta tagcaacttc
     7381 taataaaagt atggtattat tttctacgac tggattcgaa gctgatttct ctgtcaatgg
     7441 aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc ctgatatcgt
     7501 tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga tcagtcagac
     7561 gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg ctattaccgt
     7621 aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg tcatcaacga
     7681 tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg atgaactagg
     7741 ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg ccatcggcgt
     7801 aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc ttgagatcat
     7861 ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg ctactgctaa
     7921 aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac aagtagatgg
     7981 ttttattcta gtgtattcag atagtcaaga tactgtcatt gattatttat accaaaacca
     8041 catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg tttatgtaga
     8101 taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga atggacataa
     8161 gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac gatatttcgg
     8221 ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag tactagagca
     8281 tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca cagcattggt
     8341 ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt atggctatgt
     8401 cattccagag acactctccg tcattagctt caacaactcg atcttctcaa cattaacaca
     8461 cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg ctatgcaaaa
     8521 gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca tccctcatcg
     8581 cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag ctgactcact
     8641 aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat actacttcta
     8701 acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
     8761 gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt
     8821 cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag ttaaacgctt
     8881 ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt caataaaaaa
     8941 ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg caagtaatgc
     9001 aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca cggtacgaga
     9061 gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt tgataagcca
     9121 acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag atactgttct
     9181 ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa acgagaaggt
     9241 cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata tttgatcagt
     9301 aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat actcatgatc
     9361 ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac ataaccattc
     9421 atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa agaaagaacg
     9481 agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt gattgccccc
     9541 ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct cgctttcatg
     9601 ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg tcctaatatc
     9661 gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat accagcgata
     9721 tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc tgataaaggc
     9781 gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat tgcatcattg
     9841 aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat cgttgtaaat
     9901 gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg taaaaagaaa
     9961 cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc agataaaatc
    10021 catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat ccctggagtg
    10081 attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa tctgccataa
    10141 aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg gaatccgatg
    10201 atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc ttttaaagaa
    10261 atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca tgaaaaaa
//
LOCUS       DAZULG010000094        10192 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000094 DAZULG010000000
VERSION     DAZULG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.096"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..464)
                     /locus_tag="ACG9CY_002176"
     CDS             complement(144..464)
                     /locus_tag="ACG9CY_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4938675.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(612..2846)
                     /locus_tag="ACG9CY_002177"
     CDS             complement(612..2846)
                     /locus_tag="ACG9CY_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4938676.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3325..3591
                     /locus_tag="ACG9CY_002178"
     CDS             3325..3591
                     /locus_tag="ACG9CY_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS4938677.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3591..5318
                     /gene="ptsP"
                     /locus_tag="ACG9CY_002179"
     CDS             3591..5318
                     /gene="ptsP"
                     /locus_tag="ACG9CY_002179"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4938678.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            5482..6528
                     /locus_tag="ACG9CY_002180"
     CDS             5482..6528
                     /locus_tag="ACG9CY_002180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4938679.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6545..7768
                     /locus_tag="ACG9CY_002181"
     CDS             6545..7768
                     /locus_tag="ACG9CY_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4938680.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7857..8744
                     /locus_tag="ACG9CY_002182"
     CDS             7857..8744
                     /locus_tag="ACG9CY_002182"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4938681.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8830..9060
                     /locus_tag="ACG9CY_002183"
     CDS             8830..9060
                     /locus_tag="ACG9CY_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS4938682.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9117..10082)
                     /locus_tag="ACG9CY_002184"
     CDS             complement(9117..10082)
                     /locus_tag="ACG9CY_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4938683.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3239 a         1801 c         2059 g         3093 t
ORIGIN      
        1 atttgaaaag caattttcgt gaatttcagc ttatttccga aggagttgct tctgctttcg
       61 ccgtttatac gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt
      121 taattattat gttcaaaatt tgtttacacg gctaatgtag cgcctaattc atgttttgga
      181 atcgagattt caacaaggcc tgccaattcg attactttga tttcatcctc gtggtaaacg
      241 gcgtctttga ttttatctaa gaaataattt ttagcatctt cgatttcttt cggctggatt
      301 ttacgtttgt cattgatcag cagaatttca ttatgttcag gggcttcagt gtagacaata
      361 gtggtatttc cggctgtgta gaccttgact agatgggcat cagtgttttc aagctggttt
      421 aatacaagac gggaatggct gtttgttaca tttaccaatt tcatggcgct cacctctttt
      481 atgtattttt aattgcttgt ttctcttatt ataatttctt ttttataaaa tgaacagtat
      541 tttgactttt ctgaaaaaag aatgagacgg catttctcaa agaaataccg tctcacgcta
      601 taaaactgga tttattcagc cgtttcttcg gctgattctt tagccaaacg ttctggttta
      661 gaagtaacga tgatttttcc atctttatcc agttttgctt tcaactcatg gatagaaggg
      721 tgatcaagat aaaattcagc gataccatct tcgatttgtt cttggatcgt gcgtcgaaga
      781 ggacgagcac ccatagatgg atcatagcct aagtcaacta atttttcttt gacattcgta
      841 ggaacttcga tgtgaagttg ctgtgctgcc agcatttggt tgacatcgtc tagcattagt
      901 gtgacgattg tcatcaagtt ttcttttgat aatgcactga attcgatgat cccgtcaaaa
      961 cggttcagaa attctggtgt aaagtagttg tttaattggt tcaatacaga acgagtaaca
     1021 ccttcgcgtg ccgcaccaaa accaacgttt gcttctactt taccagttcc ggcattactt
     1081 gtcataatga tgatcgtatc tttaaagctg accgttcttc cttgtgcatc tgtcaatcga
     1141 ccatcatcta agatttgcaa gaacatatgc aaaacatctg gatgagcttt ttctacttca
     1201 tctaacaaga caagactgta aggatttctc cgtacttttt ctgttaattg accagcttct
     1261 tcgtatccga cataacctgg aggagaacca atcaatttag agacactatg tttttccata
     1321 tattcagaca tatcgaaacg aatcattgaa tcttgtgaac cgaatagttc gtaggcaagt
     1381 tgtttcgcaa gctctgtttt acctacacca gttggtccaa caaataagaa ggagccgatt
     1441 ggacgattct gtttgtttaa tccaacacgg tttcggcgaa tcgctttcgc tactcgatct
     1501 acagcgttat cttgtccgat gacatgagct ttcaagtcat ctgctaagtt tttcagttgt
     1561 gtctgttctt tttcttttaa ttcacccact ggaatacctg tccgttgttc aacgattttt
     1621 tccatatctt tttcggtaat cacaggtgtt tcctcttcag tgagctgacg ttctttcatt
     1681 ttttgaagtt tattgatctg atcgcgataa taagctgctt tttcgaaatc ttcttctcga
     1741 gaagctaaaa ctttttgttc ttctgcttct tgcagttttt tctcgattgt tttaggatca
     1801 acgatttgga tcgtcaagtt tttcttagaa cctgtttcat ctagcaaatc gatggcttta
     1861 tccggcaaga agcgatcttg gacgtaacga ttggataaag ttgcggctgc ttcgatggcc
     1921 tcatcagtat attttacatg atgatagtct tcataacgtt tttgcaaacc tttcaaaata
     1981 ctgatcgttt cttcgacagt tggttcatct acccgtacag gttgcatacg acgttctaaa
     2041 gcagcgtctt tttcgatgat gcggtactca tttaaggtag tagcaccaac catttgcaat
     2101 tctcctcgag ctaaagctgg ttttaaaata ttaccagcat ccatatttcc atcaccagca
     2161 gaaccagcac cgacgatttc gtgaacttca tctatgaata ggatcacatt ttctgcttga
     2221 cggatctctt caatcaattt ttgcatacgt tcttcaaatt gtcctcgtat accagtacct
     2281 tggactaaag atacgacatc taaacgaatg acttctttgt ccattaattt ttgcggtaca
     2341 tcaccatcta cgattttttg tgctaaacct tcaacaactg ctgttttacc aacacctggt
     2401 tctccaatca agacaggatt gttcttggtt cggcgattta aaatttcaat gactcgtttg
     2461 atttcgtcat cgcgtccgat cacaggatcg atatcacctt gtcgtgcttg ttctgtgata
     2521 ttgattccgt attctcccaa taaaccttct gtattgcctt gaggtggctg accaccattg
     2581 aagttgttgt ttccgccaaa ttgtgttggt ggtgtttgct cataatttcc ttgctgttgc
     2641 atttggcgag acatggaacg gaaaagatcg tccaaactgc caaatccaaa tggatcattt
     2701 tgggccatat tagtcagacc tccattttgt tgatttttga ttttttgata acagctctga
     2761 cagtaatcga gttgtgtccg ctgaccattg acagtggcat acaagtgaat cgttgcctca
     2821 ttcttgccgc agttttgaca aagcatgaat cattcacgtc ctttcacaaa aatgggtaca
     2881 tccatatttt acatctcgtt tttcatgagg taaaagaata acccctagtt ttttaagaga
     2941 tttatgaggt agaaaaggtg aaatctctat tgaccattcc tgacctttga ctcttattat
     3001 actaaaaata aaaagggaag caagtaattc gtttcatatg aaaacaaaga gggtcaaagg
     3061 aaagaatgat aaacgtcagg agaagagaaa gcgacaagaa aaaggaaata aacacgtttg
     3121 ttataaaatg tgaagaaaag gatgaaaaag aatccggaat aatttgaata cttttatctg
     3181 aaattgaatg aaaactgttg tatctaacga aaaaaaatgt taatctttac agatgaaagg
     3241 gtggcatttg ttaggattct ctgaattact aaaaaagaaa accctcacat aaaaacaaca
     3301 ctcacttaaa ggagaccgat taatatggaa aagaaagaat ttcacgtagt agcagaaact
     3361 gggatccacg ctcgtccagc tacactatta gtacaaacag caagcaaatt taactctgat
     3421 gtaaacttag agtataaagg taaatcagta aacttgaaat caatcatggg cgttatgtct
     3481 ttaggtgtag gtcaaggttc tgatgttact attactgcag aaggtgctga cgaagctgat
     3541 gcaatggcag caatcgttga aacaatgaag aaagaaggct tatctgaata atggttgaaa
     3601 tgctaaaagg gattgccgct agtgacggtg tagccgttgc aaaagcttac ctgctagttc
     3661 aaccggattt aacattcagt aaggcaacag tagaagatac tgcggctgaa gaagctcgtt
     3721 tggatgctgc attagctaaa tctactgaag aattgcagca aattcgtgaa aaagcagcgc
     3781 aaagcttagg tgaagcagaa gcgcaagtat ttgatgccca tttgatggtt ctttcagatc
     3841 cagaaatggt aggtcaaatc aagcaaaata ttaaagacaa cagcgtaaat gctgaatcag
     3901 cgctaaaaga agtaacggat atgtatatcg gtatgttcga agcaatggaa gataatgctt
     3961 atatgcaaga gcgtgctgca gatattcgtg acgttgctaa acgtatttta gcgcacttat
     4021 taggcgttac tttaccaaat ccatcaatga tcaacgaaga agtggtcgtt gttgcacatg
     4081 acttgactcc aagtgataca gcacaattag atcgcaattt tgtaaaagcg tttgtaacag
     4141 acattggtgg acgtacttca cattctgcta tcatggcacg ttctttagaa attccagcaa
     4201 tcgttgggac aaaagaaatt actgctaaag tcaaagaagg cgatattttg gctgtcaacg
     4261 ggatcgaagg cgatgtaatc atcgatccta cagatgaaca aaaagctgaa tttgaaaaag
     4321 caggtgcaga ttacgcagca caaaaagcag aatgggaaaa attaaaaaat gcagaaactg
     4381 tcacagctga cggcaagcat tttgaattag ctgcaaacat cggaacacca aaagatctag
     4441 ttggtgtcca taataatggt ggagaagcag ttggtctgta ccgtactgaa ttcctgtata
     4501 tggattctcc agacttccca acagaagatg accaatatga agcttacaaa gctgtattag
     4561 aaggtatgga aggaaaacct gtagttgttc gtacaatgga catcggtgga gataaagaac
     4621 taccatatct gcaattacct catgaaatga atccattctt aggatatcgt gcattgcgta
     4681 tcagtttatc tgaacaaggc gacgaaatgt tccgtacaca aatgcgtgca ttgcttcgtg
     4741 catctgttca tggtaattta cgtatcatgt tccctatggt agctacactc aaagaattcc
     4801 gcgcagcaaa agcgatcttt gaagaagaaa aacaaaaatt gataagtgaa ggcaaggaag
     4861 tatctgatac gatccaagtc ggcatcatga tcgagattcc agcagcagca gtcttagcag
     4921 acaagtttgc aaaagaagta gatttcttct ctgtaggtac gaacgatttg attcaataca
     4981 caatggcagc agaccgtatg aacgaacgcg tttcttactt gtaccaacca tataacccat
     5041 caattttacg tttgatcaaa aatgtaattg atgcagcaca cgctgaagga aaatgggcag
     5101 gtatgtgtgg agaaatggct ggagatcaaa cagctgttcc attgctagta ggtatgggtc
     5161 tagatgagtt ctcaatgagt gcgacatcta ttcttaaaac gcgtagttta atgaaacgct
     5221 tggacacagc gaaaatggct gaacttgctg atcgtgcatt gaaagaatgc gatatgatgg
     5281 aagaagtcgt tgaacttgtt catgaatatg taaaataaac aacgtatcaa aagctggtgt
     5341 ggcttgtaaa agagctacgc cagttttttt gttctttttt aagaaaacgt ttttgtcagt
     5401 aagaatagac aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat
     5461 ataagtgaag ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt
     5521 gcaaaatctg gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt
     5581 atctcttata cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga
     5641 atcaatagtc ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat
     5701 gcgcattcta cagaggaaga tttccgaaat tcctttatca gatcgaacca gctttctcct
     5761 atcgtaaaaa aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca
     5821 ccttattcaa aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc
     5881 aatgggatcg acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa
     5941 tatttcaaac tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt
     6001 aaaggaatca ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg
     6061 tttggtcatg ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat
     6121 catcctgaaa atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat
     6181 tcaggtgcag atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta
     6241 gaagctttag caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg
     6301 ttagaagata gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc
     6361 caacagatta ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa
     6421 gagcgaagta tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat
     6481 gaaaaagtct ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa
     6541 aaacatgaaa atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac
     6601 atcgatcaaa acattaaaac aagaattaga aagacatggg catgaagtct atatttttac
     6661 aacgaccgat cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc
     6721 atttatttct ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt
     6781 tgccaaagaa ctagaactag atcttattca tacccataca gaattcggag cagggatact
     6841 tgggaagatg gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta
     6901 tgaagactac cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata
     6961 cttgtctaga ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat
     7021 tgatacatta cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga
     7081 cattgaaaaa ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc
     7141 actaggtctt caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa
     7201 aaatatccaa gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag
     7261 attagttatc gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact
     7321 gcagttagaa gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta
     7381 ttataaagcg gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta
     7441 tacagaagcg atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg
     7501 attgttcgat catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc
     7561 acttattgat tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact
     7621 ttatgatatt tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt
     7681 tgtttatcat gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa
     7741 aataaaatta aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt
     7801 ttctagcgta tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga
     7861 aacttggttt tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag
     7921 caggacacga agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag
     7981 aaggagctat ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta
     8041 caatggtagg ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt
     8101 ctgcagatat ctcaggacat attttagttg atttgactac aagtacacca agcctggcag
     8161 aaaaaattgc aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg
     8221 gtggtgactt aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg
     8281 tttatgatca agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt
     8341 ctgctgggaa aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga
     8401 caggaatgac ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga
     8461 ttgagacgct ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta
     8521 ttttaaaaga agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga
     8581 ttgcattaga agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga
     8641 aactatatga agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca
     8701 aactttggtg gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta
     8761 gagaaaatca atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga
     8821 ggacctccga tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc
     8881 gagtcagcag ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt
     8941 acagtaactt atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag
     9001 caatacgaat ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa
     9061 attttaggat aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa
     9121 tttagttgct tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct
     9181 aattttttgc ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga
     9241 cgtttcaaag ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca
     9301 gaggtaatca ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc
     9361 tcatctgctg ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt
     9421 tcctggatcc gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct
     9481 ttttcgaaat tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc
     9541 tttttatttc ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt
     9601 tcctgtaggt caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc
     9661 atttgataag tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa
     9721 ggcccgtatg ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt
     9781 acataattat aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg
     9841 atcaattgac actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa
     9901 tctgttatat gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa
     9961 tagctgctta ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct
    10021 cggtctttca taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac
    10081 ataggatctc cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca
    10141 agttttaggg aattctatag ttctgttcac agaaaatact ttgataatca aa
//
LOCUS       DAZULG010000095        10005 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000095 DAZULG010000000
VERSION     DAZULG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.098"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(469..693)
                     /locus_tag="ACG9CY_002185"
     CDS             complement(469..693)
                     /locus_tag="ACG9CY_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938684.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            884..3661
                     /locus_tag="ACG9CY_002186"
     CDS             884..3661
                     /locus_tag="ACG9CY_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4938685.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3742..4161
                     /locus_tag="ACG9CY_002187"
     CDS             3742..4161
                     /locus_tag="ACG9CY_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4938686.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4173..4643
                     /locus_tag="ACG9CY_002188"
     CDS             4173..4643
                     /locus_tag="ACG9CY_002188"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4938687.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4660..5439
                     /locus_tag="ACG9CY_002189"
     CDS             4660..5439
                     /locus_tag="ACG9CY_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4938688.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5426..6247
                     /locus_tag="ACG9CY_002190"
     CDS             5426..6247
                     /locus_tag="ACG9CY_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4938689.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6264..7313
                     /locus_tag="ACG9CY_002191"
     CDS             6264..7313
                     /locus_tag="ACG9CY_002191"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4938690.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7326..8330
                     /locus_tag="ACG9CY_002192"
     CDS             7326..8330
                     /locus_tag="ACG9CY_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4938691.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8963..9982
                     /locus_tag="ACG9CY_002193"
     CDS             8963..9982
                     /locus_tag="ACG9CY_002193"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4938692.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3358 a         1650 c         2092 g         2905 t
ORIGIN      
        1 cttttttgct acgtaatttt tgtaaaaaag tgggttgaga gaaatctaat tgattttcaa
       61 agaaggagta tgaaaatgag aaaaatattt attcgtttat tgtgggtact atcttagagg
      121 aataaataat agtggagaaa actatggttt ggcgcagatt ctatcaggtt tgatggaacg
      181 aagacaacct cccgacatca agtcaaaagc cacttgagat tttgatctga aaataaaaaa
      241 agaaaacaag gtaggaacat caatgtttct accttgtttt cgtaatcaat gggaaaaaac
      301 taagagtggt gtacgtaatc aataaaaagg atggtgacaa cagtacctct ttactctctg
      361 aaagcatcat aactcgtttt tcctcattat gccaataaat tgttgttata ccacatataa
      421 ggatttgtaa tagctaaaaa cagacaggaa agttgaaatc tgtgaatctt attttgtatg
      481 gaaggattta tagaacgcaa aaatcaagat aaagatgaat aaaaatcctg tttcggctat
      541 atattgcgta tctacgccaa taaaacgagt ttgttctgtc atagccatat aaagcaaagg
      601 caaaaataca actacagcga gataagatcc gatgatttct cgaaaagttt tccaggaagt
      661 atgtttcctg tttttatatt tatccgattt cattgaatag tcatagcctc cttattcttt
      721 catatgttga aattccagta taaaaactgg aaggaactgt acgtttttta gaataaaaat
      781 tataagggat aagtaggctg ctgtctataa taagatatat ttttcaattc tttattaaag
      841 attttattga caaaatgaat ccgttttcta tgatggagga ggggtgaata agttgttgaa
      901 gaaggaaatt ttatgttact tagagaacca gacagccttt ttcgatttag aacatatgaa
      961 cgaggtcttt acagcacaaa ggttagctga tcattttggg gtaaaaagaa acacagtcag
     1021 tcattatttg aaccagttaa atgatgagca actactagtc aaaatcaatt ctcgaccagt
     1081 gatttatttc cacaaagaag cttttgaaaa acaatttttt aagttacgca cgaattttta
     1141 tttttctatc gatgatctca aagaggaaca gccttttttc gcgcaaccaa aagacttatt
     1201 ctcttttatg atcggatacg acgccagttt gaaagaaagt atcgaacaaa tcaaaacagc
     1261 tctttactat ccagatggcg gattaccact tttgattaca ggcgaaagtg gcacaggtaa
     1321 aagttattta gttaatttgg tctatcagta ttgtctgctt catgatctgc tagaagattc
     1381 tgcaccattc atcactttta attgtgcaca atatgcagat aatcctgaac ttttgactag
     1441 caatttgttt ggacatgtta agggagcgta cactggtgca gaagaaaata aaaaaggggc
     1501 ttttgaagca gcagatggtg ggatcttgtt tttagacgag gtacatcgct taacaccaga
     1561 aggtcaggaa aaactgttca cctatcttga tcaaggtgtg atttatcgta tgggagaacc
     1621 taatttgtca cgccggatca aaacacgtct cttttttgct acgactgaag agattactag
     1681 tcatttctta acaactttca ttcgaagaat acctatccga atcgaactac ctgcacttaa
     1741 tcagcgtagt cgtaatgaac ggctagaact gatttactct ttttttctcg aagaacaaag
     1801 aaaaatgggg agccctttgt cagtcagtgg accagctttg tcacttttaa caagtcagaa
     1861 attatccgga aacataggag agctgaaaaa tatcgtcaaa gtttcagcag caaaagcata
     1921 tgctgaacag cgtgaacagc ctgaaatcca tgtcacgatc catcatttgc aaaaagaact
     1981 acttgcgcag cctattacta aaaatacagc acagcaagga atctatatta cgcaagacca
     2041 gaccttagag caattaatgg agaagcaaca gcccgaacag cagcgaatcg ttaaaagttt
     2101 tgagcaaatc ctaattgatt tcaagaaaaa taactgtctg ctatattctt gcgaaggaaa
     2161 attaaagcaa gaagtgattc aattatttga ttttttgctg tttgagacca gtagacaaga
     2221 aaaacacgag ctgctggttt attggacaca atctatccga gagacctttc gacaaatgga
     2281 aactgcctat caaatcaaat tcgatggaaa cagtgtctat gccttgagtt actatctcta
     2341 tcagcgaagc actatcaaat ggttaccgga agaacatgat attatccaac taatcaaaga
     2401 attagaacaa caagtttctc agtcttatcc atctagttat cactatgtcc aacgaattct
     2461 ggaattaagt aagatcaaat tagatattga agacacaagt atggatcgga ttttactgac
     2521 gatctatcta aaaaaagcga aatggtcaaa agaaaaacga ctgccaaaag cgatgattgc
     2581 tgctcacggt tatgcaactg ctagcagtat ggcaaatgta gtaaatcgtt tattacaaga
     2641 ggatctattt gaatcatttg atatgccact agatgtcaca ccacagcaga ttgcttcaga
     2701 aatccttgat tattgcgaaa acagcgatgt ttcaaatgga ttgatcatat tagtagacat
     2761 gggatctctg aaagaaatcc atcagttttt taaaaaacag ttatccgtac cgcttttgat
     2821 tttgaataat gtgacgacac ctttagcaat cacagtaggg gaatgcctgc aaaaaaatgt
     2881 atcattagaa gagatagcag aagagactgt tcgacagatc cagccagaat ggcgactgct
     2941 ctatccagag gaaaacaaac ccaaagcact cattactact tgcttcactg gaatcggtac
     3001 ggcggtccac ttaagtgaac tgctagaaaa aagcttacct acggcttgtc agttgaaaat
     3061 catcccatat gaatatcagc aattaaaaga taagaaaaac agtgacccat tattttccat
     3121 atacgaagtc gtaggaatga ttggcaccac agatcctgat atcaccaata tcgactatct
     3181 ttcattagaa gacctcatct cgggggaaaa gatgtctgta ctagctgaat ggttggacac
     3241 tactatgaat gcttcagaga aggaaatatt caatcagcgc atcattcgta atttttcatt
     3301 ggaaaaagta ctggattccg tgactattct agatacagag aaggtgatgg gagaaatcga
     3361 tcattttatg agagaattag aggtgcagtt gaatcgaagc atcagcaatc ccaaaaaatt
     3421 agcgttgtat gtccatgtga gctgtctcat tgaacgattg atccgacaaa tgccaatcga
     3481 aacctatcaa ggattagaga aatggaagca gtgtcaaaaa gaagggttat ctgctattaa
     3541 atctgcgttt agtgtcattg aagagcatta tagtgtcatg atccccaatt ctgagattgc
     3601 ctatatctat gatattttga gtgaagatac agagtttctc tcgatagaag aagaattttg
     3661 aacataagca agttgttgcc actaattttg gcatgcaact tgctttttta gtatgtgtaa
     3721 gtgaaaagag gagatgaaaa agtggataga gaaattatct tagcttccca tggaaaattt
     3781 gcttcaggga tattagattc attagagctg atctatggga aaaatcattc tattactgta
     3841 ttcgattgct atactgatga gaattttgat ttagcggaaa cagtaaggca gctttttatg
     3901 aggtacaaag ataaagaaat cattgttctg acggatcttt ttggcggaag tgtcaacaac
     3961 gaattcttac aatatatcaa tcgggatcat gtctatctcg ttgccggatt gaatttgcca
     4021 ctattgattg agctagtatc tcggttggac gctgaagtag agacaactgt tttgatccag
     4081 caggtgctaa ctgattcaaa aaaaatgatc cagttttgta acgagcggct caatcagaaa
     4141 atagaagaag acgaatttta ggaggaagaa aaatgattgt attgacaaga gtcgaccatc
     4201 ggttattaca cggacaagta gctttttcat ggacacaaac aataggcgca gactgtattt
     4261 tgattgctaa tgatgatgta cctacgaatg agatacgaaa gacaacgatc aaattagcca
     4321 agccacaagg agtcaaacta gtgatcaaat caattgatga ctcgattgcc gcgttgaaaa
     4381 gcggggtgac agataaatat aagttattca tcgtagtgga atcgatcgag gatgcatata
     4441 agctggcaga agcgtatcca gaaatcaaaa ccatcaactt aggagggatc aaggcaaaac
     4501 ctggtactcg cagcatcgga aaagcggtaa atattttacc agaagaggag gaacgcttga
     4561 aacgattaat tgacaaacaa atagaagtag aaatccgtca agtaccagca gataaaaaag
     4621 tggatgtaag agaagttttg taagaaagga agaacctcaa tgattgtaca agcagtctta
     4681 ttaggaattg ttgcatttgt cgcacaatca gaatatgcac tagggacatc gttgctttct
     4741 cgtccgattg tgacggggct gtttacagga atcgtgttag gtgatgtaaa aactgggatc
     4801 attatgggcg caactttaga gttagcattt atcggatcgt tctcagtagg tgcttcgatt
     4861 ccacctgatg ttgtgaccgg tgggatttta gggacggctt ttgctattac agctggagcc
     4921 ggtacagaaa ccgcgctttt actaggtttg ccgattgcta cgttgacatt gatattgaaa
     4981 aatatttact tagggctttt gattccaatt atgaaccata aagcagacaa ttatgcagaa
     5041 gaaggaaatt ataaaggagt cgagcatatg cacttgcttg ccggtatcgg tctttccttg
     5101 atgcttggtg ttgtggtaat ggtttcttat atggtgggaa gcaatacgat cggaaatcta
     5161 ttaaatatga ttcctgactt cgttcaaaag gggttgtcag tagctacagg gttgattcca
     5221 gctcttggtt ttgcgatgct tgctcgtttg ctgatcaaca aacaagttgc accatatttt
     5281 ttcttaggtt ttgctatagc ttcttactta gagattcctg tgaccggagt agctattttt
     5341 ggtgcgattt tagcagttgt ggtagtcaat attatgaatg tccgtcaact accacttcaa
     5401 acaactgcag gggaggtaga agaagatgaa gaattctgag atcaaaaaca gcaaaataac
     5461 gaaaaaagag ctaaatcacg tattttggcg ttcttttcaa atggagtttt cttggaatta
     5521 tgaacgtcag atgaatatgg cttatgttta tgcaatgatt ccgattttga aaaagttata
     5581 tcagtcgaaa gaagaaatgg cagcagcttt gaaacggcat ctagagtttt ttaacacgac
     5641 cccacatatc gttacactga ttttaggaat cacggctgca atggaggaag aaaataagga
     5701 tgatcctgaa tttgatgtga ctgcgatcga tagtatcaaa acatctttga tgggacctct
     5761 tgcagggtta ggagattctt tcttttgggg aactttgcga ttgattgcca caggtgtggg
     5821 tacctcttta gccatgcaag gaaatatttt aggccctatc ttgttcattt tgattttcaa
     5881 tattccgcac attctgtttc gctatctagc aacaggttgg ggatatcgct taggaacggg
     5941 ttttttgaaa aagatccagg caaacggtat gatggaaagt ttgacattag gcgcgtctat
     6001 tattggactg atggtcgttg gagcaatgac tgccacgatg atcaccatca atattccttt
     6061 aaaaatcggt tcaggagaaa atgctgtgac tgttcaaagt atttttgatg atatcgttcc
     6121 aaatatctta tgtcttggag catttggcat agtgttttat ctattgaaga aagaagtgaa
     6181 accattaacg atcttgatcg gactagcttt ctttggaatt tttggttcgt ggatagggat
     6241 tttttaaaaa aggagcagag aaaatgttta cgatgcaaga ttatatctat gaagaaaagg
     6301 aagtactaag tacgatcttg aaaaagaatg atttttcgac aagagaacat atgaaaaaaa
     6361 cagtgaattt actgatctta gcaacgggtt catcttataa cgcttgttta gcagcgaagc
     6421 cagcattgga atcttatggt gatcttactg tcgatattca agaacctttt tattttttca
     6481 attatggaaa actttctcct tcaatcgata ctgtgattgc tgtgtcacaa agcgggaaaa
     6541 gtgcttctac tgttgaagca gtaaaaatga tacaaaaaca agggttacct gttgttgcta
     6601 tcacaaatga cgtgcaaagt ccacttgctt tagaagctgc acaaattatt gatttaggtg
     6661 ccggggtaga atcagtaggc ttcgttacaa aaggctattc tgcgactgtt ttacagttac
     6721 tactcttagg gataggaatt ggaataagta aaaataaaat cagcaaaaaa atagaacaag
     6781 actacatgca acagttgcgc aaaataatca accatttacc agcgattatt caaaaaacgg
     6841 aagaattttt tgatggatat caaagtttat ttcgtttagc acagcgtttc gtcaccattg
     6901 gatatggtcc aaactgggga acagctaaag aagcagagac gaaattgact gaaacaatcc
     6961 gcgttccgtc acaaggattt gagttagaag cttatatgca cggcccatac ctggaggcag
     7021 atgcttcgca tttgctattc tttattgaag gggaaagtgt aaataaggaa cgttcgcaaa
     7081 agttgcaaag atatatgagt cggtacgttg gagagactct caccattacc acaaaaaaag
     7141 caagaaacga gaaaacactt ggattagcaa tagaatgtga tgaatatctg tctgtattag
     7201 cgcttgtcgt tccgtttcaa ctattcgctt ataaaaccgc agtagctaaa ggtattgact
     7261 tgaacaaaaa gatttttgaa gactttgata cagtcttgaa aagtaaatta taagaggtgg
     7321 aaatcatgct gaaattcaat gaagaagaac aaatcaaagc aataaaaggc gcactagctt
     7381 tacgtccgca agtagaagaa atcatcgata aactttatgc ggaaaaattt gacgctgtct
     7441 attatctagg aatcggtgga acgtatgctt cctcgatgca agctgtcact tatatgaatg
     7501 ggaaaagcaa cttgcctgtc tttgtacagc atgctgcaga atattacact actggaaaca
     7561 aaagattaac aaaagattct atcgttattt tgtcctctgt taccggtaca actcaagaag
     7621 tagtgaaagc agtagaacaa atcaagaaag taggagcgac actgattgga tttatcgata
     7681 aagcgaacag taagttgtct cagctatgcg attttgtggt cacttatcca gctcctggaa
     7741 cagaacaaat caaatttttt atggcagcag atcgattgat gtatttgaat ggagaattcg
     7801 aagcttattc agaatactat gaacagctcg aacgttattt accaacaggg ttagtggaag
     7861 cagagaaaaa agcagatgca tttggcttat catttgcaga aaaacatcga cgtgatagta
     7921 tgcattattt tattggagca gggaatcaat ggggagctgt ttattcatat gccatgtgtt
     7981 actgggaaga acaaagctgg cttccatcga aatctatcca tgctgccgag tttcttcacg
     8041 ggacgttaga gatcgtagaa gaaacaaccc cagtcacatt atttttaggc gaagatgaac
     8101 agagagtgtt atctgaacgt gtggctaaac ttttgccaaa aatttgcagc aattatacat
     8161 tgatagacac aaaagactat ccagtagaag gtatcagtga aaaatatcgc ggacgtgttt
     8221 tgtctttctt attgatgcat gtagtgacac aacgcatcga tgctcatgtg gagcagttga
     8281 actgccatcc attagagata cgccgctact atcgtcagtt tgattattag gatagactgg
     8341 ataaaaagaa tataaaaaat aaagagagca gcgataactg ctccctttat ttttttaaaa
     8401 tattggcatc aattacgtta taaaacacat tttttgcatt atctacagtt caacttataa
     8461 tctgtttaat gggacatgtg ggtgccaacc attttttgtt atgcaatagt actatttagt
     8521 tgttgcatgg atccgtagta aaagtccaag tacaacgagt tgcgccagtt tacatagcgt
     8581 tttgtatcca gaatgttgac taaaacaaaa gttcctatgt caaccgtcaa cagagacaat
     8641 ctgtccgttg caggacctga cagatttaga aatgttcaat ctgtctctta tgtattttaa
     8701 atcaatactc actaggagaa gtttcctaac ctcctaatag taatgctggt agtaacattg
     8761 tcaaaactgc ttcctcttag gaacaagcaa agcataacga gtgtaaaaat aaaatgtaga
     8821 ctattgtaac atatcattga ttatttcaag taaaaacgat aaaagaagta aacaaacact
     8881 taaaaacaat ggaaattagt cccaacttta agaaatgtat actataatgg aaattatcaa
     8941 ttatttattt ggaggaatat ccatgaaaca aaaaatcgca gttcttggtc ctggatcttg
     9001 gggcactgct ttagcacaaa cattggcaga aaacgggcat gatgtccgta tttggggaaa
     9061 tgtacctgaa cagattgatg aaatcaataa gtatcataca aatcagcatt ttttacctga
     9121 tttgacgatc ccagaatcga ttatcggata taaagaattg gcagaagcag tggataacgc
     9181 agatgctatt ttatttgttg ttccaacaaa agcgattcgt tcagtcgcac aggaacttat
     9241 tggtaaaatg aatactaagc caatcattat acatgcaagt aaaggactag aacaagatac
     9301 gcataaacga atctcagaag ttttggctga agaaattcca gaagaaaaaa gacaggcaat
     9361 tgttgtatta tctggtccta gccatgctga ggaagtggca gtacatgata tcacaacaat
     9421 tacagctgca agtattgatc aaaaagcagc agcctatgta caaaaactgt tcatgaatga
     9481 ttatttcagg atttatacaa ataatgatgt gattggcgta gaaactggtg cagcactgaa
     9541 aaatatcatt gcaattggtg ccggcgctat ccatggatta ggattcggag atgatgcaaa
     9601 agcagcaatc atgactcgag gattggcaga aatcagccgt cttggtgtcg cgatgggtgc
     9661 caatcctctg acatttatcg gtttgagtgg tgtgggggac ttgatcgtta cttgtacaag
     9721 tgttcattct cgcaattggc gtgccggtaa cttgttagga cagggacata agctggaaga
     9781 agttctagaa aacatgggaa tggtcgttga gggtgtggca acgacaaaag cagcagtaga
     9841 gctagctcaa cagctaggtg ttgagatgcc aattactcaa acaatctata atgttttgta
     9901 taatggagaa gatatcaaaa aagcagcaaa agaaatcatg ttacgtgacg gaaaaatgga
     9961 gaatgaattt tctcttcgtt aaatagatga tcaatagtta atcgt
//
LOCUS       DAZULG010000096         9591 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000096 DAZULG010000000
VERSION     DAZULG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.099"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..417
                     /locus_tag="ACG9CY_002194"
                     /pseudo
     CDS             1..417
                     /locus_tag="ACG9CY_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(421..1140)
                     /locus_tag="ACG9CY_002195"
                     /pseudo
     CDS             complement(421..1140)
                     /locus_tag="ACG9CY_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(1091..1300)
                     /locus_tag="ACG9CY_002196"
     CDS             complement(1091..1300)
                     /locus_tag="ACG9CY_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938693.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(1282..1350)
                     /locus_tag="ACG9CY_002197"
                     /pseudo
     CDS             complement(1282..1350)
                     /locus_tag="ACG9CY_002197"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            1577..2850
                     /locus_tag="ACG9CY_002198"
                     /pseudo
     CDS             1577..2850
                     /locus_tag="ACG9CY_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2957..3988)
                     /locus_tag="ACG9CY_002199"
     CDS             complement(2957..3988)
                     /locus_tag="ACG9CY_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938694.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3995..4825)
                     /locus_tag="ACG9CY_002200"
     CDS             complement(3995..4825)
                     /locus_tag="ACG9CY_002200"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4938695.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4822..5637)
                     /locus_tag="ACG9CY_002201"
     CDS             complement(4822..5637)
                     /locus_tag="ACG9CY_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4938696.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5634..6458)
                     /locus_tag="ACG9CY_002202"
     CDS             complement(5634..6458)
                     /locus_tag="ACG9CY_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFS4938697.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6448..7548)
                     /locus_tag="ACG9CY_002203"
     CDS             complement(6448..7548)
                     /locus_tag="ACG9CY_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4938698.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7726..8511
                     /locus_tag="ACG9CY_002204"
     CDS             7726..8511
                     /locus_tag="ACG9CY_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS4938699.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8544..8927
                     /locus_tag="ACG9CY_002205"
     CDS             8544..8927
                     /locus_tag="ACG9CY_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4938700.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9003..9134
                     /locus_tag="ACG9CY_002206"
     CDS             9003..9134
                     /locus_tag="ACG9CY_002206"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4938701.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9103..9339
                     /locus_tag="ACG9CY_002207"
     CDS             9103..9339
                     /locus_tag="ACG9CY_002207"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4938702.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            9351..9437
                     /locus_tag="ACG9CY_002208"
                     /pseudo
     CDS             9351..9437
                     /locus_tag="ACG9CY_002208"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(9417..9557)
                     /locus_tag="ACG9CY_002209"
                     /pseudo
     CDS             complement(9417..9557)
                     /locus_tag="ACG9CY_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
BASE COUNT         2807 a         2272 c         1859 g         2653 t
ORIGIN      
        1 gttccatgag ttttatttgt gttgatggga caacccatca gctatttacg atccttgaag
       61 atcgcagact ttacaaacta actcaatact tcctgcactt tcctagaaaa gcccgtttaa
      121 aggtcaagta cctagaatgc cagctattgt cagctgctta aaacggtctt tccaaatgcg
      181 gagatcgtaa ctgatcgctt tcatattgtc caacatatca atcgcagctt caatcaattg
      241 cgtatccaga tcatgaatcg ttttcgagcc tcgcactctg aaaatcagaa gaagtaccgt
      301 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
      361 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
      421 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
      481 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
      541 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
      601 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
      661 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
      721 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
      781 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
      841 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
      901 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
      961 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
     1021 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
     1081 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
     1141 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
     1201 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     1261 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     1321 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     1381 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     1441 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     1501 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     1561 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     1621 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     1681 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     1741 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     1801 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     1861 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     1921 ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt ctaaacattg
     1981 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     2041 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     2101 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     2161 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     2221 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     2281 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     2341 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     2401 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     2461 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     2521 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     2581 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     2641 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     2701 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     2761 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     2821 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     2881 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     2941 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     3001 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     3061 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     3121 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     3181 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     3241 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     3301 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     3361 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     3421 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     3481 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     3541 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     3601 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     3661 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     3721 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     3781 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     3841 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     3901 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     3961 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     4021 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     4081 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     4141 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     4201 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     4261 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     4321 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     4381 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     4441 acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc gttaccaaaa
     4501 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     4561 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     4621 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     4681 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     4741 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     4801 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     4861 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     4921 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     4981 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     5041 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     5101 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     5161 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     5221 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     5281 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     5341 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     5401 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     5461 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     5521 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     5581 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     5641 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     5701 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     5761 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     5821 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     5881 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     5941 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     6001 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     6061 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     6121 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     6181 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     6241 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     6301 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     6361 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     6421 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     6481 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     6541 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     6601 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     6661 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     6721 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     6781 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     6841 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     6901 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     6961 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     7021 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     7081 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     7141 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     7201 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     7261 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     7321 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     7381 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     7441 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     7501 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     7561 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
     7621 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
     7681 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
     7741 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
     7801 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
     7861 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
     7921 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
     7981 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
     8041 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
     8101 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
     8161 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
     8221 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
     8281 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
     8341 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
     8401 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
     8461 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
     8521 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
     8581 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
     8641 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
     8701 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
     8761 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
     8821 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
     8881 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
     8941 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
     9001 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
     9061 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
     9121 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
     9181 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
     9241 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
     9301 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
     9361 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
     9421 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
     9481 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
     9541 aaaagctcta ttctggctct ttgtcaaatg atgttggtaa agagaaatct t
//
LOCUS       DAZULG010000097         9527 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000097 DAZULG010000000
VERSION     DAZULG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.100"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG9CY_002210"
     CDS             168..362
                     /locus_tag="ACG9CY_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938703.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG9CY_002211"
     CDS             631..930
                     /locus_tag="ACG9CY_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938704.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG9CY_002212"
     CDS             complement(1027..1461)
                     /locus_tag="ACG9CY_002212"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4938705.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG9CY_002213"
     CDS             complement(1693..2403)
                     /locus_tag="ACG9CY_002213"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4938706.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG9CY_002214"
     CDS             complement(2393..3253)
                     /locus_tag="ACG9CY_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS4938707.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG9CY_002215"
     CDS             complement(3258..3896)
                     /locus_tag="ACG9CY_002215"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS4938708.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG9CY_002216"
     CDS             complement(3911..4210)
                     /locus_tag="ACG9CY_002216"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4938709.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG9CY_002217"
     CDS             complement(4253..5737)
                     /locus_tag="ACG9CY_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4938710.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG9CY_002218"
     CDS             complement(5757..6218)
                     /locus_tag="ACG9CY_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4938711.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG9CY_002219"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG9CY_002219"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS4938712.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG9CY_002220"
     CDS             7521..8291
                     /locus_tag="ACG9CY_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4938713.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG9CY_002221"
     CDS             complement(8564..9391)
                     /locus_tag="ACG9CY_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938714.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2978 a         1942 c         1488 g         3119 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtat
//
LOCUS       DAZULG010000098         9495 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000098 DAZULG010000000
VERSION     DAZULG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.101"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..2835
                     /locus_tag="ACG9CY_002222"
     CDS             160..2835
                     /locus_tag="ACG9CY_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFS4938715.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGIAKIEATFVEAQKKIWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3017..3256
                     /locus_tag="ACG9CY_002223"
     CDS             3017..3256
                     /locus_tag="ACG9CY_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938716.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLAFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNGRVAKEWYLVHS"
     gene            3272..4645
                     /locus_tag="ACG9CY_002224"
     CDS             3272..4645
                     /locus_tag="ACG9CY_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4938717.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGGKADRPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLAYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEAHEAKVVRHIFQLYLTKKYGYKKLCQRLTQQKFFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKTIFLQAQEIRQSRCTAKKDTYPYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKHKVIQQKKQEAQWLKAFEEGNCSLEELKQQLQALQ
                     KPRSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDHVCRIEVTKEKTVCGIYFRQMTQ
                     NILEGNE"
     gene            4649..6286
                     /locus_tag="ACG9CY_002225"
     CDS             4649..6286
                     /locus_tag="ACG9CY_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4938718.1"
                     /translation="METTPTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMSYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPKPILVKGTHEPIISEEDY
                     QKVQERLVLEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQQGEPIQVKDVQRILTSLDRFLAQSEKKQVKALYRTFIEKITFDPNYKEILEITM
                     KFTPAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6449..7381
                     /locus_tag="ACG9CY_002226"
     CDS             6449..7381
                     /locus_tag="ACG9CY_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938719.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNI
                     NYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
     gene            7497..7817
                     /locus_tag="ACG9CY_002227"
                     /pseudo
     CDS             7497..7817
                     /locus_tag="ACG9CY_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            7995..8354
                     /locus_tag="ACG9CY_002228"
                     /pseudo
     CDS             7995..8354
                     /locus_tag="ACG9CY_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            8418..>9495
                     /locus_tag="ACG9CY_002229"
     CDS             8418..>9495
                     /locus_tag="ACG9CY_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4938720.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNG"
BASE COUNT         3358 a         1467 c         1869 g         2801 t
ORIGIN      
        1 aaaagaaaaa agttgtgatt ttggaaatca caactttttt cttttgtcaa gattttgagt
       61 gggatatatt tttgtagaac tgaagatcat gaaattatga gaggttggag gtaatgtagt
      121 catacaaata aaaatttttt agaaagtgag tggtaattaa tgaaaatgaa tcacacttca
      181 attcagaagc atttacaact gttaaataca ctggggattt cttatgttaa tttgagaact
      241 ttttctacat taaacaagaa aaccgtttct catatttttt ctactgcaga ttctcaagca
      301 attattaact atttagaaaa acagaaattg tctgaaggta cagtaaacat aacttataat
      361 cctattaaac aagaggtgat tacacaaaaa aagcattctg tgagagacaa tgatattgaa
      421 aaaattcggt ttgtatttat tgatattgac cctaaaagga aagagggaag tgcaacagat
      481 agcgaaaaga aaaaggcaga aaatgttatg gaacaagtag aacgttattt gaaagaaaag
      541 agaatagaat catttgtaaa agttgatagt gggaatggct accatatttt tcttcccata
      601 aatgaacaac caaataatca cgagacgatt cttactattc agaattttct tcaattactt
      661 caccgaagat ttggagtaga agatgaagtt gatattgata cttctgttta taatccttca
      721 agattatgca aattagttgg tacaccagcg gtcaaaggaa agtccacaaa agagcgtccg
      781 catcgtttat ctagtttcct ttctattccc gataacatag atagaagtaa ttgttttaaa
      841 attcttgaac agatagttga agaaaattcc atgatgtcaa cctctagagt aaaaagccat
      901 ccatctttta gagaagtgtt accttttatt caagcagact ataaacaatg gttagctcac
      961 tatcctgaat taacttatac cataaaagag ggcgatcata aaggagttac cttacttata
     1021 tttgacgaat gtccattaag aaaacacacg aataattcta atggttcttg tttgtctgtt
     1081 acggctgata ataagattcg atttcattgt ctgcatgctt cggataaaaa taaagatatt
     1141 caagactttg taaaaaaata tccgttgcca aaacctgtaa atccttctgt agaatcatta
     1201 gaatccaatc aagaatatta ttttgaccag tttaaactga atcaagaagg agtatttttg
     1261 gtgaaagaag gtcaagaaga aaaagttgct tgtccgatgt ttattaagca aatacgcaaa
     1321 aatatcgaac aaaaagtcat tacacttgtt cttgcctatt atgtcgatca ttgttgggat
     1381 gaaatagaaa tagaaggtgg atgtttacaa actaataatt ttaaacaatt agcaaaacat
     1441 ggcttaatca ttaaagctag aagagaagct gaagtcgttg actttttaca gattcaacgg
     1501 gagaatgctc caatcttttt tgaacatgaa tctcttggct ggaaaaaaga aaatgaagca
     1561 atgatttttc agttacaaga ttcttttgga cgtgaaattc cttctgtatt aactaaaaac
     1621 tcatgctacc aattaacaac taatggtaca gaagcggaat ttgacgcatt ggttcataag
     1681 tatgttttag gaacgaactt agaacttgct tggtcgattg gtattgcttc tattgtgtta
     1741 ggctatttaa acgttacgaa ggctaatact tttttgagtt tactaattaa cctacagggc
     1801 aagagcacaa cgggtaaaac cactacgtta cactttattg cctctttatt tggaaatcca
     1861 actacggttc ttagaaatat gaatgctacc aataaagcca ttataaagct agcatcaaac
     1921 aatcagggag gtgttccact aatactagat gagttaggag cagcaactca aaataacttt
     1981 agctcgttga tctatcaatt agcttcagga gaagaaaggc taagattaga ttctaattgt
     2041 cagcttatgc cacaagagac tttttctgtt ttgaccctta cctcttctga agagcgtttg
     2101 gaaaattatt tcaatgagaa attaactggt ttgaaagttc gatatatcga attttctgat
     2161 atcaagttta caaaggatgc gaatcacgca gagaaagtta aaagtctttc ttctaatact
     2221 tttggttggg gtataaagtt atttcttttg aaactctttg aacaagggat tgcaaaaatt
     2281 gaagcaactt ttgtcgaagc tcaaaaaaaa atatgggctg ttttacctga acattctttg
     2341 aagcaacgca ttggtaataa tctagctttg atttatagtg gagctctcct aattcaagat
     2401 atactgggct atcctttacg attagagcat atacagacgg tgcttattaa ttcgtacgaa
     2461 cagttaattg tagatactag tttaggaagt gagcaaacgg tatttgaccg agtgaaaacg
     2521 ttgttggtga tgaatagcca taaattttta acaaacagtg acaacaaaca tacgcccagt
     2581 acgatttggg gaaaagtatc aattcaaaaa aatggggata ttaaggtaaa tatttttcct
     2641 atcccgtttg aggacttgtt aaggcgagaa tttcaagtaa aggatctaac ctatcttttt
     2701 agacaattgc ttgattcggg taatatgtgt gcagaaaaag ggagaagaac gaaacgtatt
     2761 cacatgaatc gtcaagttct tccaacatac gagattctat taccttcttc actcaaatcg
     2821 tattttcgat tgtaatttag aataaaccaa agtatgaaag tgaggcacca tgttatggaa
     2881 atatttctta ttgatttagc aaaacaactg attacttatc tgtatgatga cgagcaaacc
     2941 caaacggaag taaatgaagc ttgatctatg cttcatttac ttcattcaat caaacattta
     3001 agaaagggga attctaatga ctaaaaataa aatgacacta aaagcagagg ttcttctgta
     3061 tattcaagaa catttttcta atcaagcctt ttttacaaaa ccaatttact tggctttcga
     3121 aataagaggg gtatctgctg gatctattgg aggtactctt caagcgttga agaacgaagg
     3181 atatttagaa aatcactttg tgcaacgttc gttcaatgga agagtcgcga aagaatggta
     3241 tttagttcat tcatagagag gtgattcaag catgcgtgtg gcttgttatg atcgaacgtc
     3301 tgtttatgaa gaagcgaaaa tcagtaatga cgctcaatat gatgcagtga aagagtatgt
     3361 tttagagaaa tatgaacaaa ttcctcaacg attttcagac aaaggagaat caggtggaaa
     3421 agcggatcgt ccagcttttc gacagcttag agcttgggtt tgtgcgtcag aagaagaggg
     3481 ggtgatctat gtctggcgtt atgatcgatt ggcacgtcaa gccaaaatag ccttagaatt
     3541 tattgagttg tgccaacaac atcaggttca agtttattcg atttctgaac ctcttcctgc
     3601 ttctttctcg cacgcgtcaa cgaataaact atttgtgcaa ttattgttca cttttgcaga
     3661 acaacaacga aacgtcatta ttgaaaatgt tcgtgccgga cttgcctata aaaagcaaca
     3721 acggcaatat ttgtcttctg cggttccata tgggtatcgt ttagtgaatg ggaaaatcca
     3781 acaagaagca catgaagcaa aagttgtccg ccatatcttt caactttatt tgactaaaaa
     3841 atatggctac aaaaagcttt gtcaacgatt gacgcaacaa aagttcttct tccgtgaacg
     3901 tccttttcag ccctatcata tttatagtat cctaaaaaat ccattgtact atggcgaaat
     3961 caaaggagga tctttgggga agtatctagg aacctttgaa ccgattctct cgaaaacaat
     4021 ctttttacaa gcccaagaga ttcgccaatc aaggtgtaca gctaaaaaag atacgtatcc
     4081 gtacttatta cgtcaaaaaa ttcggtgtcc cttctgtggt cgtcatctga gttcgaagta
     4141 tcaatggaat acaaagaaga cgaagacgct tcattattat cattgtaccg accgaagctg
     4201 ccaaggaatc tttcttcggg cagaaagggt tgagtcagaa gtattggaaa agatacaggc
     4261 tttttttcaa tacgaaggcg tctatcaagc cttgattgcc caattgacgc aacaattagc
     4321 cgaaaagcga aaagatgaaa aacacaaagt aatccaacaa aagaaacaag aagcacaatg
     4381 gctcaaggca tttgaagagg gcaattgttc actggaagaa ctcaagcaac agctgcaagc
     4441 tcttcaaaag ccgaggtcta cttcttcgtt tctttcactc caacagtgtg aagcaaaagt
     4501 gaaagaatgg cttcaactaa aagacctctc tagtcgacaa ttgttatgtg accatgtttg
     4561 tcggatcgag gtaacaaagg aaaaaacagt ctgtgggatt tacttccgac aaatgacaca
     4621 aaatatttta gaagggaacg aatgatctat ggaaacgaca ccaaccaaac gagtcgcatt
     4681 gtatattcgg gtgagtaccc aagaacaagc tttagaagga catagtattc aagcacaaga
     4741 acaaaatgga cgacagtttg ccgaacggat gagctatgaa gtggtagaag tttatgccga
     4801 tgaagggatt agtgggaaat ccacaaaaaa tcggttcgcc tatcaacgaa tgatgaacga
     4861 tgctcggaac catcgttttg atctactagt gatttggaaa ttgacccgtc tcggacgcaa
     4921 tatgttagat gtcttacaaa ccgtcgagga actgttgcga tatgatgtgg ggttacattc
     4981 gatctcggaa caatttgacg tgactacttc gacaggaaaa ctaatgttac aactccttgg
     5041 gagtttcggt gaatttgaac gcaaccaaat tgcggagaat gttcagatgt caatgcaaag
     5101 cttagtgaaa gataaaaagc gttatgcagg tggacgacgt ttaggctatg tcagtggtat
     5161 tgacacgaat ggtcaaaaac aattggtcat tgaaccagaa gaggcgaaaa ttgttcgtct
     5221 gatctatgca aaatatttag gcggagcagg gtatcgagcg attgccaatt ttttgaatcg
     5281 tcaaggatat caaacagtca aaaagaatgc tttttctacc acggcagtca aagatatctt
     5341 acgcaataaa atttatgcag ggtatttaga atatgcacgc tatatcaatt gggaaaccaa
     5401 acgacgaaag ggcaagaacc caaaaccgat tttagtgaaa ggtacccatg aaccgattat
     5461 ttcggaagaa gattatcaaa aagtgcaaga acgccttgtt ttagaaggca aacagcccaa
     5521 atggaaccat acaggtgaaa atgtattgac aggcttgtta cgttgtccag aatgtggggc
     5581 accgatggca gcgagtaatg tcacaaatac tctaaaggat ggcacaaaga aacgaattcg
     5641 gtattattct tgttcagtct ttcggaataa aggagcaagt gtgtgtcatg ccaattcgat
     5701 tcgtgccgaa caagcggaaa catttgtcgc cgaccgatta aaggagatcg ttcagctacc
     5761 agaagttctt ccccgattgg tggcagcgtt aaatgaagaa atagtgcgac aaagccaacc
     5821 attggaacaa gaactcgtgg tgttactgga acgaaaggaa gaattgaaga ctaaaattga
     5881 aaaatgggaa gctgctttgg aagatagtcc tgaattgttt ccgatgttaa aagaccgctt
     5941 agacgaattg actgaaaaac gacgacagtt gcacattcga gaaaatgaaa tcttagggat
     6001 ctttcaacaa caaggagagc cgattcaagt caaagatgtc caacggatat tgactagttt
     6061 ggatcgtttt cttgcccaga gtgagaagaa gcaagtcaaa gccttgtatc gtacctttat
     6121 tgagaaaata acctttgatc ctaactataa ggagatattg gagattacaa tgaaattcac
     6181 gcctgccgtg gtacaacaat tgaatgagca atatcaaata gccgtatcca aaacaaagga
     6241 tacggctatt tttgtgttaa agacaccctt tacgttaacg atctagtcta ggttatatca
     6301 aacaacgtta gaggggcgct aacgcttctc tagcgatttg aaatcgttag atgagtcgtt
     6361 agggaggtcc ttatcataaa tagggatatt tattaatgaa tatttgaaat ataattgtat
     6421 ataagaatta aaaagagtgg ggatagatat gaaaaagatg ggtaagtgta ttttatgtga
     6481 aaaatacact gaacttacta aagaacatgt accacctaaa aaatcaggga atactggagg
     6541 taaaaagacc agaactggta atttagatga tttccttaag agcgatttta caaaaggtga
     6601 ttttccgaag gggattaaaa ggaaacctca aggaaatgtt tattatacgt tatgtagtaa
     6661 atgtaatagt ttttttggta gtgagtatgt tgaggaatat attagatttg cagaagacaa
     6721 taaaaatttc ctttataaca atacaagttt aaagaatggt aagtctgatt taacacattc
     6781 aataaagaaa atgaattcac ttagagttgc aaaagcaatt gtagcaatgt tttttagttt
     6841 aaatggggat gaagattcaa tggataagcc attcttagat tcggtacgag aatatcttag
     6901 caatcctaaa agtactttat ttcctcatga agactacaaa attataatga attattatct
     6961 tccaataggt gctccacaga attctaaaat caaaattcct aatgacattg taaatgatgg
     7021 tgacactcac aatgtaataa aaggttttat gccccttgat ctatcaactg ggagaaaaat
     7081 tcgattttca gaaattcagt atgaaggaat tggttttact cttatagatt taaaaaattc
     7141 aacttttgaa ataaaagaag gattagattt aaaaaatctc ctttgtgcag atgataaaat
     7201 caaaaaaata caattgtaca aagtccctat attaacacca acagtactta caatgttgga
     7261 gatggaaaat aatattaatt atattgactc aggttcagat ttgctgatta ggtggcaggt
     7321 aataactaat tttcttatgg aattaaaaaa atacagtaat actcaaacga agttctcttg
     7381 aagttataaa gaatacaata aatataaatt tcctttagac tatattaaaa tcagcaatcg
     7441 aaggaaaata aacgaagaaa atggtacgca aaatctaact attagagcaa aaacaggaag
     7501 ctgctttgga agatagtccc gaattgtttc cgatgttaaa agatcgttta aatgaattga
     7561 ctgaaaaacg aagacagtta caaattcgag agaatgaaat cttagggatc tttcaacaac
     7621 aaggggaacc gattcaagtc aaagatgtcc aacagatatt gactagtttg gattgctttc
     7681 ttatccagag tgagaagaag caagttaaag ctttgtatca cacctttatt gagaaaatca
     7741 cctttgatcc taatcagaag tagatattgc cgatcccgat gagattcaca cctactgtga
     7801 tttgataatt gaatgagtaa tatcaaatag ccgtatccaa accaaaggat atggctattt
     7861 ttgtgttagg gatatacgtc acgtagcttg ttatcaagaa taagtttctt tatttttttg
     7921 ctttttctaa aactgagtat gctaaaataa gagggtatat agcaacttta aataagagat
     7981 aggagtgtat ctttttgggt gcagaattaa atcagaagtt atttagtgcg gcagataatc
     8041 tccgtagtaa aatggatgcg tcagaatata aaaattattt attggggctg attttctata
     8101 aatatttatc cgaccgtttg ttggaacaag tggttctttt agcagatgaa tccttagagg
     8161 aatatgacac agtaagcaaa caaacgatgt tgtatcgtga attactttca gatgaagaat
     8221 caaaagaaga tctgattgca acaatcgttg atattttggg gtatgcgatt tcgccagagt
     8281 atcttttcaa cgtcttagcg gatcaagcaa aacaagcaac gttccaattg aatgatctaa
     8341 acaaagcctt tttagcaagt catatttatg gaaaaatact gtcggcaaaa ttcgaatagt
     8401 gtcgaggagg tgctgtgatg gaagaattaa aagaacgcat tgtcaattat gtcatagaga
     8461 actctgagat taaaaataaa tgggggaaca atacagcaca gaaaatatct gaaaaattaa
     8521 atgttagtcg taatattgtc tctcaatatt tgaatgaatt cgtaaaagat ggagaatttg
     8581 gaaaaatcaa tactcgtcca gtaatcttct tttctttacc ggagaaagtt ccaaatcaac
     8641 aaatttttag ctcttcagag gaagcggaaa aatggtatga tagctctttt agggttggaa
     8701 tagaaagggt tatcggaagt caaggaagtc tgcgaaatgt aattgctcaa attaaagcag
     8761 caatctacta tccgccaaat ggcttaccca ttttacttca tggcgagact ggaactggaa
     8821 aaagctattt agcaaattat gtgtataagg atttgttggc caataattat atagaagaga
     8881 aggcatcttt catttcagtt aattgtagtg aatatgccaa taatccagaa cttttcttgt
     8941 ccaatttttt tgggcatgta aaaggtgcat atacaggagc cgaagatgga agaaagggtt
     9001 tagcagaatt agctaataat ggagtcttat ttttagatga aattcatgca ttgagcaatg
     9061 aatgtcagga gaaacttttt cagtttatgg atacgaggaa atttcatcgg atgggtgata
     9121 atgaaaactg gtacacatca agttgctgga ttatatttgc aacatcagaa ccacctgagg
     9181 ataaactatt atcaactttg ttgagaagaa tacctgtaaa gctaacactt ccaacattag
     9241 acagtcgttc tctttatgaa agaaaacagt taatcaagat attttttaaa tcagaagaag
     9301 aaagactagc aaagaaaata ctgatatctc aacaattatt agatttttta ttaaactatc
     9361 gctttagtga aaatgttggc aatttgaaaa atatcattca attgacctgt gccaatgcct
     9421 tttatttctt agatgatttg gaagaacttt atattcagat gtatcattta ccacaatggg
     9481 tgaatttcaa cggca
//
LOCUS       DAZULG010000099         9414 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000099 DAZULG010000000
VERSION     DAZULG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.102"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(212..1264)
                     /locus_tag="ACG9CY_002230"
     CDS             complement(212..1264)
                     /locus_tag="ACG9CY_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4938721.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1277..2215)
                     /locus_tag="ACG9CY_002231"
     CDS             complement(1277..2215)
                     /locus_tag="ACG9CY_002231"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4938722.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2666..3316)
                     /locus_tag="ACG9CY_002232"
     CDS             complement(2666..3316)
                     /locus_tag="ACG9CY_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938723.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(3416..3925)
                     /locus_tag="ACG9CY_002233"
     CDS             complement(3416..3925)
                     /locus_tag="ACG9CY_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS4938724.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(4126..4995)
                     /gene="scrK"
                     /locus_tag="ACG9CY_002234"
     CDS             complement(4126..4995)
                     /gene="scrK"
                     /locus_tag="ACG9CY_002234"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4938725.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(5015..6136)
                     /locus_tag="ACG9CY_002235"
     CDS             complement(5015..6136)
                     /locus_tag="ACG9CY_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4938726.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(6138..6533)
                     /locus_tag="ACG9CY_002236"
     CDS             complement(6138..6533)
                     /locus_tag="ACG9CY_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4938727.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(6552..7349)
                     /locus_tag="ACG9CY_002237"
     CDS             complement(6552..7349)
                     /locus_tag="ACG9CY_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4938728.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(7351..8139)
                     /locus_tag="ACG9CY_002238"
     CDS             complement(7351..8139)
                     /locus_tag="ACG9CY_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4938729.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(8153..8629)
                     /locus_tag="ACG9CY_002239"
     CDS             complement(8153..8629)
                     /locus_tag="ACG9CY_002239"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4938730.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(8701..>9414)
                     /locus_tag="ACG9CY_002240"
     CDS             complement(8701..>9414)
                     /locus_tag="ACG9CY_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4938731.1"
                     /translation="NQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKKILEDSFPRDT
                     DLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIEDMILGMNISKF
                     SNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDAIYILQNDLGI
                     SFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYY
                     SVDIPIHEVKYVYDYVKRA"
BASE COUNT         2973 a         2004 c         1349 g         3088 t
ORIGIN      
        1 ctcattttaa cacatatttt tattctagtt gccttttgaa caatcaataa cataaaggtg
       61 agtttcttct acattttaaa aaatcgaatg gcactgaaga gttcagttgt cagcatcaat
      121 ctcctactca aaaaggcaaa aaaacgtaag tcagatgttg ttgacatctc acttacgcat
      181 ccattgattg aaaatttctt agtaaaatct actagcctaa aaatttgaag acttcttctc
      241 gatcatacag tttaaatact ttctcgcata aaaattgagt aattagtgcc gcaaaaacag
      301 ggatgactaa ccaacaaaga acaacaagaa agccatttag ccctgcatct aaagaagcca
      361 acggaccaac aagtccgacc acgccgaaac cagcagaagc aggcgtacct gaaatagaaa
      421 atagagcgac aggaatagct gtaatcactg cactaactaa acaaggaata agaatcacag
      481 gttttctaaa tagattaggc atcatcatct tcatagcgcc taaagcaatc gcaattgtca
      541 cacctgactt attaaccttc caagagttca ctactaaaac aaaagtggtt gcagctacgc
      601 ccatcgcagc cgcaccagca gaaacaccat ctagttggat tgccattccg attgctactg
      661 ttgaaatagg agagataatc aaaaaggcaa aggaacaacc aatcaagata gacattaaca
      721 gcggttgtaa ggtagtgaag ttattaatca atgatccaat tgcagaagta attgctgtta
      781 caaaagggaa taaataaaaa ccaagtaaac ctgcacctac acctacaaca atcggtgtta
      841 acacaatcga cactgaccca aaactatttc caatcagtaa aatcagcccg acagcaatcg
      901 atgctgtcag catcgtatta ataatatcgc ctgtaccagc accaacgtaa acacccgttt
      961 cacccaaatc aggtgtgaat ttgactacac cagaaccaac aaaagaagct ccggctacaa
     1021 ccatcattct catcggatca aatccaaatt gtaaggcaat caaaccacca atgatcaaag
     1081 gtgttgctag ttgaaaaata actgctaact gtgcgatcgt taccgcaaag ggatacgcac
     1141 taaaatattt taaaatggca cctaatactg cattaggaat caatccaata atgattcctg
     1201 ttgctgtacc agcgaggact ttattcagaa aaattttcgg tgatattttt tgtgaagttt
     1261 ccattcaatc tctcctttat tttacgttca aactatcttc ttttgcatgg acaagctcag
     1321 tcaagaaatc acaataaggc gtcgcaacac catatttctt cccttttcta gaaattgcgc
     1381 cattgatata atcgatttct gttaatcgat ggttcttaat caagtcttga tacattgaag
     1441 gataatgtaa cccaattgtt tccgggtcaa aacaagattc acaatgtgca atgacttctg
     1501 ggacatctag ttcaatcttc tctactgctg ctactttggc aaactcattg acaatcgtcg
     1561 ccaccatttt atgagcagtc gatgtttttc ctagctcagc catattaacg tctaagattg
     1621 tgcatagtcc gttcattgtt ccgttcacac atgctttgcg ataaatagaa tagtgaatat
     1681 tgtcagaaaa atgagcattt aatcctgatt cagacaattt gtctgctaat ttttttgcag
     1741 ctgcttcttt tccatcacct aaattttgta attctactga accacttcca aataatttga
     1801 cctgaccagg accttcaaga cctgccgtcc acatggtatt tccaatatag atattttcca
     1861 ttggtacaaa tttctcaata atatcttcat gcccgatacc atttaataga cataagacct
     1921 ctgtatcttt tttgattaat gattggatat cctgcagcat tttttccagc tgcatcgctt
     1981 tggtaaatag aataatcaga tcaacttgat cttctttttc tacttcggat tgaagaacga
     2041 ttggtagttt tgcttctacc tctttaccat taaaatttgc ttgtaatcca tgctctttga
     2101 tttgttggac atgttctgcc cagccatcga ttaataatac ttcgttccca ctttggtgaa
     2161 gcattaaccc aaagcgactt cccattgcgc cagcgcctgc aattgctatt ttcattgatt
     2221 cttcctcctt tttctttcta ttatattcat ttgagaatca tttcacaacc ttgttttgtt
     2281 attcttctct tatttatcat gtttatatcc accattcaag ttattttatc agcaaatcaa
     2341 taaaaactgg aatagaatcc caatttcatc gtaagagaaa ctagactagc aataagaaaa
     2401 tagaaacatt catagaaaaa acctcactcc tctgattata aaagaggggg cattaacctc
     2461 gtgtatacta aaaccttcca ttcattcgat ggatgatttt ctttttgtga aaaaatattc
     2521 attgtagtac gatgttgcgc aacacagacg gatggtattg cttattattt attaaaatcg
     2581 aacaatacac ttatttaaaa acttctttcc cagattttct tccattttta cagttataac
     2641 tagtattatc atgaaaaata gccttttatt gtctatggga ataataattt aaaattggta
     2701 cttttttatt aattagttca tcgcccaata atctctccag tttcttagca tctcctgact
     2761 caaccggaat tatccaagcc cagtccaaat aattttttaa aatctcatca tattttgcaa
     2821 atatttcatt gccatattcc gtccttaaaa ttatattttc agtgccagac aagtgatttg
     2881 tattgagttg ataaaaaaga tctccatcta aaaaaatatc attagaagaa gatttcccca
     2941 cagtaatgac cattccatca gctctatgtg ttacatacaa ataattgtat gacctcattt
     3001 cacctaatga atcaattatt tcttgccctt tagctctaat atattgatct ctattaccta
     3061 tctcactctt atttaacaaa agttttttca tgtcaaattt aatcggctgt tttatttttg
     3121 taaattcgct tctactacta atccaatccg agattaattt ttcaacatgt cccagagtaa
     3181 actctgattt attcgctaaa tacttttttt gttgtattaa agacatcttt ccccgaagct
     3241 caaaattgag cctacatcga ttctctatct cttcaaaaag catcttatct tcatcattct
     3301 tatattcatc attcataaaa ccaatccagc tcctcgatta agataatatt ttaatctcat
     3361 acaaaaacta gctctcatca aaacaaccat tgttttgatt tctcatgaaa tttctttata
     3421 cttttctccg attataaatt cctaacccct tgagaaaatt acgcatatat ttatctccgc
     3481 aaggcttaaa atttcgttgg ccttcttttc tcaaaaatgc cccgatttcg ccttttgatg
     3541 gatagatttc tgcctcagca aaaacgtcta aaatatcatc agtggtcata gacaaggcga
     3601 tttttaattt ttttagcaac acattgttga tatatttgat ttccttgctt ttttgcggcg
     3661 tgtctactat cggcttatct ttttgcggac cacgcgccaa gagaatcagg ccatttagga
     3721 atgcgtcaaa aacttggttg ttgatggttt tttcatacac attattttct gcattatctt
     3781 tgttcttatc ctgtactttg gtcagtaccg cctgagcttc ttctttagtc agtggtacac
     3841 cgcctagctc aaaagcttta atcaaatccg tatctttcaa atcaatcgca taacgcaatc
     3901 gcaataacct atcattgtta ttcatttgtt cctccgaagt atttagtctt tactttctta
     3961 cgtacttttt gtcttgctaa gatttttagg atcaatgata aaaaccgcat cacttatgat
     4021 aaggcgctgg gtatcatgag tgaatgatat tgctgaatac aaatagtccg accacattaa
     4081 gttgatcgga cttcataagc attttattga ctaatacttt tttgcttaag aattgattct
     4141 aacatctttt gctaaagtta agcatcctat agttcctgca ttatctgcta acttaggtgt
     4201 gacaatgtat tcttcgatat ttggcgtatc gacatagccg ttaattagtt ctaaaaaacg
     4261 tctttgtact ttctttttca gatgttcttg ttgcatgaca ccaccaccaa aaattataat
     4321 atctggtgaa agcatcaggg tcgtattaca ggcacattgt gcaatataat ccgcctcgat
     4381 ttcccagaac ggatcttcta tagaaagatc ttgtcccttg gttcccgtac gcatctcgat
     4441 agcgggtcca gatgccaacc cttcgagaca atcatgatga aaaggacaag taccagagaa
     4501 agtatctcct gaatgtctct taactaacgt atgccccatt tctggatggc taaacccttc
     4561 aacaaaaatc ccatcttgta tagctcctcc accaattcca gttccaacag tgtaataaac
     4621 aacactggat aaaccttttc catgacctga aacatactcc ccgtaacaag cagcattcac
     4681 atcggttgtc caggcaattg gaatttcgaa atgcttattc aacgttccaa caaaatcaaa
     4741 atttttccag gcagtttttg gagtagaggt gatatgtcca taggtctttg attcacgatg
     4801 gatatcaatc ggtccaaatg accctactcc aatgctttct aattgttctt tatagtgatt
     4861 aaagaaatca attaccaaac tcattgtttc attcggtgta gttgtaggaa aactcactct
     4921 ttctaaaacc tttaaatcat cattgccaat ggcacaaaca aatttagtcc cacctgcttc
     4981 tattgagcca tacatttctt actccaccgt ccttttatct aggaattcgt tcttccctgt
     5041 ctaatagttt ttcgaaaaac tcatgtggtt ccttttggta ccaataggca acagaagaaa
     5101 tatcgtcttg acgctcataa agcttgatgt catcatttcc tagggtttgg aaagtaacac
     5161 gtaaattttc tttgaaacta atggggtcgg gtaaatgcca gcggtacaac ccatgcatcg
     5221 gtaaagcatc atcgccaaaa ccatgaactg gatttggatt aggttttcca gcactaaaat
     5281 aatcccgggt gtgatctctt ttagattgaa aaggatagcc cataaatagc gtattaaaat
     5341 tttgagcata tgccctttct ccaaagttct ttttatggaa agcccaagcg ccgccaaaat
     5401 aatcttctgc tcctgtagat gttattgtag ggaaatcttc atctccatct atataaaact
     5461 taaattctcc ttccccccac cagtatcttt ccaatgcgca caaagcaaag taggttccta
     5521 cataataccc agtgcctttg attccatcaa taagggtgta atcttttcct tttgtcgtga
     5581 tgggttcacg gttccattta gcatgaaaat acatcgcatc atccggtact tcagtcaatg
     5641 cataattaaa tgtataaaaa atgtgcttaa taaactcagg atgttcattc gttaaagtaa
     5701 tcttagcttt ttttcgaaat ggcatctcaa aatagcaatt catccctgat gttggattca
     5761 ctacgattgg taaggaattc acttcgcatc ttgtcccaaa cccattacaa aaaaaatctc
     5821 ctaatggact ttctacagaa ggagtggatt catcatccca ataaatacgc aaaattaaat
     5881 cacgtaagac gaaactccca gcgattgttt tgtctgtcac tgtcatccaa aaatgacgta
     5941 aaattccagg gccttccatt tctgctaaag taattgtctc tccagcattt atcggaacag
     6001 aaggtgaccc ttttctccca attcctagat gactcgctgt cattgccgct ttcccttttt
     6061 caccattttt attttctggt gaaatagttc tactttcctc tcccttgtgg agccatatat
     6121 tatttaaaca attcatttta ttcgtcctct atcctttctt cacttttttg attattcata
     6181 tagatgattt gttgttgtgc ctccgcaata attgctccaa tatctaaatt atcatttcct
     6241 aactgcaaag cgattgacat tgccaaggga agattcattc ctgcaattac gtgcactctg
     6301 tcagaaatat aggtaaaaaa cttttgattt acacttcctc cagccatgtc agttaaaaca
     6361 attatttcgt ctgtagtatc cactaactct ttaatttgtt catctatatc atcaatttct
     6421 tcgacatatg cacataagct actaatttga ttatgcatac ctgttaaaaa ctctagtgtc
     6481 tctttaaaac cctcagctag tattgaatgg ctagcaatga tcaatcttct cataattcaa
     6541 atccttttcc tttacgctaa tattccaaat gcagccataa ccatcgcaaa aacaataacg
     6601 agtagaatca aaatggtcat actgattttt ttcttactga gtagataata tgctgcagca
     6661 gtaataatta ctggaattaa tgttggtaaa attttgtcca acattggttg gattttcata
     6721 ctaacttcac caaaagtgaa tttaagtggc gttgaaacac tgataactga agcaattaat
     6781 gatccaacta ctgttagccc caatactgaa gcagcttcag ttaaataatt aatcttttca
     6841 ccgtattcag taacaatctt cattccctgc ttataaccaa attcaaagcc tctcattcgg
     6901 aaaaagaaga ttaccatgat taaaatgaac cataaaaaga ttcctacagg attattttct
     6961 aaagccatat aagccgcaat tgatcccatg atggttggga tcatcgtcat caaaagagag
     7021 tccccaatac cagcaattgg acccatcgta ccaactttaa agtcattaat cgcatctttt
     7081 gcttctaatc cttgcttttc ctccattgca acacaagcac ctaaaataat ggctgccagc
     7141 caaggctgtg tattgtaata ctgaaagtga ttatttaaag cttcgatgta ttcatcatca
     7201 tctttgtata attttcttaa aataggtgac aatgcgtaag tcacagatgc accttgctgt
     7261 gtttgataat taaaggtaca aactgacatc aaccaacgat agttcgcttt cctaagatcc
     7321 tttttcgtta cttgatattt ttcgctcatt ttattcatcc tccccaataa acccaacatc
     7381 agaagctccc gcagaagcag tgatttgtgc atctttttca gctctatgat acacattaaa
     7441 tgctaaagcg aatcctatga tcgccgctcc aataataggt accttcaaaa aagcggctaa
     7501 gaaaaatcca acaagtaaat aactaatgta tcgacctgta ggcataaagc gtaacaacat
     7561 cgcaatacca acagcaggca acatttttcc ggctaatgtt agcccatcca taaaccaatt
     7621 aggtaaaaca tccaaaacgt aattaacagc agactgtcct aaagtaacgg caataaatac
     7681 tggaattgct gcagataaac caaagaaaat tggccccaac cacaaaacac tattcatttt
     7741 cttaaattct cgtttattcg catattcctg tgatttgcga acaacaaaac catttaagat
     7801 tttgacaatt acatctagtt gcacccctaa catacctaca ggtagtccta ttgctaatcc
     7861 ggcttctact ccaagctggc taccaaatgc cgttgcaata attgcgcacg ttccatagtc
     7921 cggtacagat gagccaccaa aaccggcaac tcctaatgac atcaattgaa ctgtaccgcc
     7981 aactatcaaa cctgttttta tatcacccat caaaattcct gtgaataatc cccaaaaaac
     8041 tgcgtaattt acaaagatca tcggaatccc ataataatca acatgtttaa taaatgctaa
     8101 tatagtcagc ataataattt gaaaaatagt aaaatgcatt atccttactc ctttatccta
     8161 attttgattg aatttcctgt gccttatccg ccggaacaaa ttgtgtaaca atttctacac
     8221 cagaatccaa caatttttta tacgtgctga tattttcatc tgatgcatag gctctagaag
     8281 atagctgtaa tgcattatct ttaacaacag taccgccaac aattagtcgg tcaattctaa
     8341 taccgttatc tactaaatga aggattgttt ccggctcttt cacgatgaca aatacttttt
     8401 cctgatcgta tttccccttt ttaaaattcg caatcgctgt ttcttcagtg ataattgata
     8461 cagccattcc agcaggtcta gctaaacgca tacttgattt ttggatgtcg ttatttgcga
     8521 cagaatcatc gatcaccatc actctgtttg ctttagacat tggtgcccat tgcgtgacaa
     8581 taatcccatg caataaacgg ttatcaactc tcgacataac aatactcata aatacttcct
     8641 cctttatctt tcattttctt acaagcaatt accatgccaa aacaaaaaga acttgataaa
     8701 tcaagctctt tttacataat cataaacgta tttaacctca tgaatcggga tgtcgacact
     8761 ataataatgt tcaacgacac tgaaagattg cttaaaatgt gtctgaaatt tttgaaattc
     8821 ctcactcgtt tcattgaaat aaatttcatc ttccaaagta ttttgtttta ctaatcgttc
     8881 aattaagcaa ctaatgtgaa catataatcc gaaacacgta ttattatcaa atgaaattcc
     8941 tagatcattt tgtaagatgt atatagcatc tgaaacccgt tctaataatt tagtcggatt
     9001 taatatggtc aattgctcca ttaaattcgt tagactaaaa ttcttcagaa tattattgtt
     9061 caaaactttc agttcattat cattaaagtg tctattcaat tgattggaga atttacttat
     9121 attcattcct aaaatcatat cttcaattga aaagaattct acatcttcta gatgtggatt
     9181 tagtgttcca acaataaaaa gaatattgtt ctctttacga agctccgata aatagttttc
     9241 attcactaaa gagtaatagt cacacgtttc tatgacaaga tcagtatccc ttggaaagct
     9301 atcttcaagt atctttttta atttaaacga agttcctaat ccgcttgcac aagaacagac
     9361 aatcatatcc ttcttaatat ttgatgattc ataaatataa tccacatttt gatt
//
LOCUS       DAZULG010000100         9397 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000100 DAZULG010000000
VERSION     DAZULG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.103"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            187..2022
                     /gene="tdc"
                     /locus_tag="ACG9CY_002241"
     CDS             187..2022
                     /gene="tdc"
                     /locus_tag="ACG9CY_002241"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4938732.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2327..3805
                     /locus_tag="ACG9CY_002242"
     CDS             2327..3805
                     /locus_tag="ACG9CY_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4938733.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3771..5102
                     /locus_tag="ACG9CY_002243"
     CDS             3771..5102
                     /locus_tag="ACG9CY_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4938734.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5216..7768
                     /locus_tag="ACG9CY_002244"
     CDS             5216..7768
                     /locus_tag="ACG9CY_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS4938735.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7775..9214)
                     /locus_tag="ACG9CY_002245"
     CDS             complement(7775..9214)
                     /locus_tag="ACG9CY_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938736.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9189..9371
                     /locus_tag="ACG9CY_002246"
     CDS             9189..9371
                     /locus_tag="ACG9CY_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938737.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
BASE COUNT         2953 a         1567 c         2005 g         2872 t
ORIGIN      
        1 attgttcgct tttttgcgtt gaattatcag aaaaatccga ctttatttat ttttgtttgt
       61 aaaaatctac gtggatttga tgttataaaa aaaaaattct catttttggc gctatagtga
      121 agaggtgtta cagataaaca catgtatctg caagacattg accaacaaaa tatggaggga
      181 agaaaaatga aagatatgga tatcaaggcc gtctttatag gcgacaaagc tgaaaatgga
      241 ccagtttata aaatgttgtt gaacaagatg gtagatgaac atcttggatg gagagaaaat
      301 tatattcctt cagatatgcc tgcaattagc gaaggagata agctcactcc agattatcta
      361 gccacccgcg accatatgat agaggtcttg gatgaagtca gtcagcgatt acgcgcagga
      421 tcgatcccat ggcattcagc aggccgttac tggggacaaa tgaatgctga aacattgatg
      481 cctgctttat tagcttacaa ctatgcgatg ctgtggaacc caaataatgt tgcattggaa
      541 tcttctatgg caacttcgca aatggaagca gaagtaggac aggattttgc ttcattattc
      601 aatatgacag acggatgggg acacattgca gcagatggtt ctattgctaa tcttgaagga
      661 ttatggtacg cccgttgcat caaatcgatt cctttagctg tcaaggaagt gctgcctgaa
      721 aaagtgaaaa aaatgtctga gtgggaactt ttgaatttat ctgttgaaga aattttggaa
      781 atgaccgaaa gctttacgga cgaagaaatg gatgaggtca aagcagcttc ttcccgtagc
      841 ggaaaaaata tccaaagatt agggaaatgg ctggtccctc aaacaaaaca ctattcttgg
      901 atgaaggcgt tagatatttg cggagtcggc ttagatcaga tggttgctat cccagttcaa
      961 gaggattatc gaatggatat taatgctttg gaaaaaacaa ttcgtgaatt agctggacaa
     1021 aaaatcccaa ttctaggtgt ggttgctgtc gtaggaacga cagaagaagg tcaagtagac
     1081 agtgtagata aaatcgtcca attaagagaa aggttaaaag atgaagggat ctatttctat
     1141 ttacatgttg atgccgcata tggcggctat gcgcgttcac tgtttctaaa cgaagcagga
     1201 gaatttgtgc cgtatgcttc cttagctgaa ttctttgaag aacatcatgt tttccaccac
     1261 tgcgtaacga ttgacaaaga ggtatatgaa gggttcagag ctatttcaga agcggattct
     1321 gtcacgatag atcctcataa gatgggctat gtcccttatg ctgctggagg aatcgtaatc
     1381 aaacataaaa atatgagaaa tatcatttct tatttcgcgc catatgtgtt tgaaaaatca
     1441 gtaaaggctc cagatatgtt aggagcttat attttggaag gatcaaaagc tggagcaacc
     1501 gccgccgcag tctggacagc acatcgtgta ttgccattga atgtgaccgg atatggtcaa
     1561 ttgattgggg cttcaatcga ggcagctcaa agattcagag aatttctcga tcatctgact
     1621 tttactgtta aaggaaaaac aatcgaagtc tatccattga atcatcctga ttttaatatg
     1681 gtcaattggg tattcaaaga acaaggctgt acagatttga acgctatcaa tgaattaaat
     1741 gaaaaaatgt tcgatcggtc ttcttatatg gatggcgatg tttatggtga acggtttatt
     1801 acttctcata ctacatttac acaagaagat tacggtgatt ctccgattcg ttttgttgaa
     1861 agaatgggtc taacgaaaga agaatggaaa aaagaacaga aaatcactct gctacgtgcg
     1921 gctatcatga ctccttattt gaatgatgat cgaattttca atttttatac aaaaaaaatc
     1981 gccaaagcaa tggaaaagaa actaaacgaa atcatccaat agaactgatt tttgtgtgaa
     2041 agaataacaa gagacatatg gagaacagac aattatttgc ttcatcaaaa caattgtatt
     2101 tccccctgtt tttcatatat tcctcgccaa gatattcttt tttataaaac gttctataag
     2161 ttggatcgtt cgtttcatgt atcaaaccca gttcactaag atcgttcaat ggatgtttcg
     2221 gtgatactgg gttttgtact gaatcgatga aaccttgtca tttctgttct tgaacaaaaa
     2281 taaaagaaaa caaaaacata tgtcgctatt gaaagaagga ataaagatga aagatactgg
     2341 ttcatcaaag caaatcacga ccatgacctt tatcggaatg acctgcgccc ttgtagcaag
     2401 tgtacgaaat attccagatg tagcagcaac gagctggacg atgatttttt acatgctggt
     2461 tgcagtatta ttgtacgctt ttccaatctc attgatctct ggtgaatttg ctggaatgtt
     2521 tccccaaaaa ggtggtccag aactttgggt atcgaatgct ctaggaaaaa aatgggggtt
     2581 cgttgtctca tggctgttat gggtccaaat gtttcctggg atggtgatgg tcgcttcagc
     2641 actcgctccg ttgtttggta atattatcga taatgtgccg cttggtttga acagcaaatt
     2701 tacattagtt gtcattttag tcgtttattg gatcatcact tttttgaatt tgaaatttga
     2761 tatggcaaaa atcggcggaa aagtcggtgt ttggttagga ttgtacgtgc ctttgacgat
     2821 gatgctctta ttagggtttg ctgcgtggat caaagttggg atcgtaccaa ccggaacatt
     2881 aggcagtttt tcatgggaca aattaatacc agatactacg actgcatcct catttgttta
     2941 ttttgcgccg atcatgttta tttttactgg tatcgaaatg tcctctgtgt atatcactcg
     3001 attggaaaat ccagttaaaa cgtatattcg cggagtattt gccgcgttaa tttttatatt
     3061 tttcgtcaat attttgaatg ctttagttgt agcaaatgtc gtaccaaaag gacaaatgga
     3121 attgaataat atcgcgcaat ctatttcgat ttattgccaa attcttggtt tgccacatct
     3181 tattgtgaat ctgtttagtt tacttgtctt cattggtgta gcagtccaat tatctgcttg
     3241 ggcctcagga ccagcaaaga cagtgacaga aagtgctaga aaaggtgcgt atccaccaaa
     3301 attcaatttt tggaagacga atcaatttga cgtatccaaa tccgttattt tgactcagtc
     3361 tgtcattatc tcaatctttg cactttttta tttgctgatt cctggcgtca atcaagcatt
     3421 tttgatgctc gtaaattcaa ctactgttat ctactgtatc gtctatgtca tcatggggat
     3481 cgcagttttg agattgcgtt atacacatgc aaagttgaat cgaccattta gaattggaaa
     3541 aaaagagaaa aagagcaatc ttggagtttg gactgtcgtt attgttttat ttgcggcaat
     3601 cggattttct gtcggactga cgatgaaagc tggaacatgg atcaatctaa ttgcagtcac
     3661 agccatcagt gtcattttgt ttgtagtacc actctgtatt gaaaaaataa agaaaccttc
     3721 ttgggaacag gaagtcgcca tgctgcttga aaaagaaaaa ggtgaacttg atggaaaaac
     3781 cggcaatgaa acggactcta actaatcgaa atatccagtt aatcgcatta ggtggcgcaa
     3841 ttggaacagg attattttta ggttcagcaa aggcaattgc atcagcaggg ccagctgtta
     3901 ttctcgttta tttgatatgc ggtttggcta tttatggttt gatgcgggcc atgggtgatt
     3961 tatttttagc aaatagtaca tttaactcta tcagtgattt cgttgactat tatttaggca
     4021 aaaagtgggc attttttgtc aattggactt attggctttg ctggcttgga gctggacttg
     4081 cggaattaac agcaatagga ttgtatatcc atttctggtt tgtacatgta ccagctttga
     4141 taagcggagg tagcgcacta atgatcttaa tggcagtcaa ccttgttgca gtcaagtggt
     4201 ttggtgaatt ggaatctttt ttctcaatca tcaagatctt ggggattttc ctttttatct
     4261 tattgggtat aggattgagt gtctcagatt ttcataccca gtctatcgga atcaaagaaa
     4321 attttggaca gatcgatgaa ttactaccgt ttggctttgc tggaatggta gtctcatttc
     4381 ctatggtttt attcagtttt gctggaattg aaatgatcgg cttaaccgtt ggcgagacaa
     4441 gtgatccgca aaagaatttg aaaaaagcaa tcaactgtct gccttggcgt attctctttt
     4501 tctatatagg aacgattttg tcgattttat ttgttgttcc atggcattgt ttagatttaa
     4561 aagaaagtcc tttagtgact atgctgcaag tgatccattg ccaaggcgga gcagcaattg
     4621 tcaatctaat gattttggtt gcttctttgt cctcagcaaa tagtgcagtc tacagtacta
     4681 gcaggatttt atatcaaaca gcaaaagaaa aaagtttgcc ccattggttt tgtgcgcttt
     4741 ctaagaaagg cgtaccttta cacggtattt taattacagg aatgttattt tttgccggtc
     4801 tttgtctaca tttttttgta gctgatagtc gattgttttt tcagcttttg gcaggcatga
     4861 caacgagttg ttttctattt gtgtggtctt cgatcttgtg tgcacatgtt caatttatta
     4921 aagaaaagaa acggaacact acacaaagat ataaagatct tgctttgttg gtgtttttta
     4981 cttcaattgc tttcagcttg ttattcgaaa gcacgacacg ctttattcct tttttattgg
     5041 ttatctggtt tgtattctta aattttgtat ataaaagaat aagcaataga aatatcagtt
     5101 aggcccagat acattttgca aaacgaatta tttcagaccc ccctgaaata atcgatacgc
     5161 ctgaaaaaac ttccctgcgt ataataacag tatcaattaa acaggagggg ataggatgaa
     5221 ctggtacaga ttgacggtag aagatgtatt gaaaaactat caaacatcaa cagaaggctt
     5281 gtcgcaagaa gaacgacaga aacgcctgac tgatcaagga tataatgaaa taaaagcaag
     5341 gcagcagaca aaacgatgga aaaaaatagc aaagcatttt actgatctgc tgatgatcgt
     5401 gttgatcatt gcctcttttt tgaaatttgc ttctagtgaa tatattgaag gaagtattat
     5461 tttattcgta gtgatcgtca atggactcgt tagttactgg caagaaagaa aagcggaaga
     5521 atcattgaat ggtttgaagc agctgatggg acaagaagcg atagtactat caaacgggat
     5581 gcaagaaaaa atccctgcga aagaactggt aaaaggagat gtcattgtct tacgtgccgg
     5641 agatgtcgtt ccagcagata tccgattaat cgaagcacat gatttattag tggaagaatc
     5701 gattttgacg ggagaatcag aagcatctga aaaaagtcat gccgttttga cagaagaaga
     5761 atcgatcggc gaccagaaaa atatgggttt ttcaggaacg cttgtacaat ctggttctgc
     5821 agtcggtgtc gttgtagaaa caggaaacac gacagaaatc ggtaaaatca atcaagcatt
     5881 gcaatcggtc gaacaacaaa cgacgcctct gattaaaaaa atcaaccgat taaacaaaca
     5941 aatttttcaa ggcattcttt gtttgagcct attcttagtt atttttacga catttcgata
     6001 tggtatggat tggcatattc ttctttcggc tacgattgct ttggtggtgt caatggtacc
     6061 cgaaggattg ccagctgttt tgaccatgat tctatccgtt ggagtacatg aaatggcaaa
     6121 agaaaaggcg atcatcaaag gacttccttc ggtagaaaca ttaggttcta tgacagtgat
     6181 ctgttcagat aagacaggaa cattgacaaa aaatgaaatg acagttgtgg atgtagctgc
     6241 aaaagaagaa gcctgcgtgt tgtccattat gaaaaactgt caggaattaa aaacaaaaga
     6301 agaacagaaa acagaaaatt taagtggcaa tccgacagaa gttgcgctga ttcgacacac
     6361 agaaacagcc aatctacctt tgaaaaaaat agaagcaaag attccgttca gttctgaata
     6421 caaatatatg gcaacgatgc atgcggagga aaaaggagcg gtgatttacg tcaaaggtgc
     6481 accagaagta ttatttgcta aatcaactct ttcttctgca gaacaagaag catggagtca
     6541 gacagctgca gaatttgcga gaaaaggtca gcgtgtttta ggatttgctt ataaaaaagt
     6601 agattcgaaa caggaactca cgcatgagac tttaacccaa ttaacttttg cagggatcgc
     6661 cggattgatc gatccgccaa aagaaagtgc agtcaaagct gtaaaggaat gccaacaagc
     6721 agggatctca gtgaaaatga ttacagggga tcacaaagac acagcaaaag cgattgcgga
     6781 acaaattggc ttaaaacata ctgccaaagt tttagaaggg atcgatttgg atcttatgtc
     6841 tgatgaagag ttgattcagc aagtgccaat agtcgatgtc tttgctcgaa cgacaccaga
     6901 acataaactg cgaattgtta aagctttgca gaaaaatgga gaaatcgtcg gaatgaccgg
     6961 agacggcgta aatgatgcac ctgctttgaa aagatccgat gtaggaattg ctatgggaat
     7021 caaaggtagt gaagtaagca aacaggcagc tgatatggtt ttaggagatg ataatttcca
     7081 tacgattgca aaagccgtaa aagagggccg gcggattttg gataatttgc aaaaaaccat
     7141 caatttcttc ttgccaactg cattagctca aggattgatc ttgatttggg cacttatgct
     7201 gaatcgcccg ctacctttgt ccccagtaca gattttatgg gtcaatatgg taacgacaat
     7261 cactttatct tatgcactag ggttcgagtc gccagatcca gaaatcatga agcgcccacc
     7321 aagagatcca aagcaaggaa tcctatctgg ttatcatttg tttcggatca tctatgtctc
     7381 cctactgatc atggttcctg cctatttatt ggctatgcag tttgaagggc aaagcttaca
     7441 gcaaaccatt cttctgcaaa atatcgtctt agctcaagct gtttacatga tcaattgcag
     7501 agagttgtcc aagccgtcat tgaaccaagg tcttttgcaa aacaaagcgc tttttctttc
     7561 attaggtatc cttgctttat tgcaagggat ggttattttt ctaccagtcg gccagcagct
     7621 gatcggtaca gtaagtttaa cgatgacaca acagttattg attggagcaa atgtgattct
     7681 tctattctta gttgtagaaa tagaaaaagg gatcatgaac aaactgtttc gcaaaaaaga
     7741 aaaaacagct agagaataca gtcgctagcc gttactataa aaaagttttg aaagtgagtt
     7801 cagtcaaacg attgagctca tttttcgttg gaatagaaga aatgtataca atagggatat
     7861 ctagagaatc tagagggaaa ttggaaatca agatatctgt tttttcaaaa gaaatagtgg
     7921 aaagatcggt tagttcgata ggtaagaagc agacacgttt tcctaagtaa tcttttaatt
     7981 cctgctgcaa gaacgccttc catgaaggtt ctccttggaa aataaaatag acattgaaca
     8041 aattcggttt gatagaaaaa atcgtttgtt ggatcagtag agacaagagt tggagaaagt
     8101 aatttggttc attgatttct atttcttttt ttagatcgtt cagtaaattc tctttctgtt
     8161 gatacaagtg tgcatattgc tgatcatatg tttctaaaaa acgatggtat tgacggtcca
     8221 atgcttcaat caatatcggc gattcatgat attttaatat gaggagagcc aagttatcag
     8281 tcagagatgt tttttttagt tgatcaagag acagctctga agctagttcc tctacaaacg
     8341 tttcgaggac ctctaaaaat ggagaatgat gcaggcagcg agcggtttct tgtccatagt
     8401 tattgttgta attccaactt tccagaaagc agaaagaagc aaaagaaagc tcagtgtctt
     8461 taagataaat accttccgtt aaatacaatt ttttcaatgc agagtcatac agggaggaga
     8521 gtgtgctgtt ggtatggata gaagaatcta atcggcaatt cgcttttata cggatcgtat
     8581 tgataaagaa ccaacatgtt ccgaaaatct cttctgtttc aaccgctagt tcagtttgac
     8641 tgaggtttct taaaaattga aaataatggg attcatggat cgaataatcc tcgaaaaaat
     8701 agttgttatg agtgaaagga atcagcaatc gatgataaaa agtacgaatc gcacgctctt
     8761 ttccaagaat acaagctgta tatttcgtca aatcgatctg caagtcgtaa cttgctaaac
     8821 gctgattgat tttagtcaca tgtcttttca atgtcccata agaaagccca tggtcggcta
     8881 gaaaatcagg gacagcaact ttttttgtaa aaagcagatt tttcagcagt tcgacactga
     8941 ctgattgttt gaaaaaaatc tcccaaaggt catttgttaa taaattttgc tggttttgca
     9001 aactgattcc tgcttgatcg ctgatgatgt cccagccttc gggcagctga gagcgaatag
     9061 attgaaggtc agaaaaaatc gttcgttcgg ttgtccctat gatttcagct aattcattta
     9121 cggttatctt tgtatgattc agcaattgtt ctactaaaga aacatgacgt cgaatatctt
     9181 tttcggtaat gatttttttc attagtgaat acatcaatca gactcccttc tattctaaga
     9241 atacggagaa aataaacggt ggtcaagttt caactgattt ttcaatcatt gtttattttt
     9301 ctggagcatt tactgatgat aagaatttca tcatgaactt gaaaaatgat accactaaaa
     9361 tcaaaaaata ggtgggcaaa caataataaa catttta
//
LOCUS       DAZULG010000101         9311 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000101 DAZULG010000000
VERSION     DAZULG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.104"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..710)
                     /locus_tag="ACG9CY_002247"
     CDS             complement(42..710)
                     /locus_tag="ACG9CY_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4938738.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            844..1131
                     /locus_tag="ACG9CY_002248"
     CDS             844..1131
                     /locus_tag="ACG9CY_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938739.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1547..3085
                     /locus_tag="ACG9CY_002249"
     CDS             1547..3085
                     /locus_tag="ACG9CY_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS4938740.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3082..4047
                     /locus_tag="ACG9CY_002250"
     CDS             3082..4047
                     /locus_tag="ACG9CY_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938741.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4034..5356
                     /locus_tag="ACG9CY_002251"
     CDS             4034..5356
                     /locus_tag="ACG9CY_002251"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4938742.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5419..5523
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5597..6340
                     /locus_tag="ACG9CY_002252"
     CDS             5597..6340
                     /locus_tag="ACG9CY_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4938743.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6355..7347
                     /locus_tag="ACG9CY_002253"
     CDS             6355..7347
                     /locus_tag="ACG9CY_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4938744.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7359..8096
                     /locus_tag="ACG9CY_002254"
     CDS             7359..8096
                     /locus_tag="ACG9CY_002254"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938745.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8086..8808
                     /locus_tag="ACG9CY_002255"
     CDS             8086..8808
                     /locus_tag="ACG9CY_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4938746.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8892..>9311)
                     /locus_tag="ACG9CY_002256"
     CDS             complement(8892..>9311)
                     /locus_tag="ACG9CY_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4938747.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3050 a         1604 c         1967 g         2690 t
ORIGIN      
        1 tgatatttct gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca
       61 agaggatcaa gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca
      121 gatccaattt ccagatactt cactttattc aatgtataag atagaataga acagctaagt
      181 aatgtataca gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa
      241 gtaatcaaga aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa
      301 atgatatctg ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca
      361 gaaagaatac ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga
      421 aggctctccc ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga
      481 aaagcgtgtt ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt
      541 gaagtgacag aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc
      601 ccaccatctg ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct
      661 gcgcctaaaa tgattccgca caattctgta aataaattta attttttcat ttctagcctc
      721 ttttcttttg aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag
      781 aggaaaatga aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga
      841 aaaatgcgag cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat
      901 caaggagttt ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat
      961 aaagcaaatc aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac
     1021 atctttcttt ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa
     1081 ctcattcctc tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata
     1141 agaaaacaag acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg
     1201 tcatgataca gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt
     1261 tctactttat tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata
     1321 gctgattcca agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga
     1381 accaaaaagt tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt
     1441 atttttttct tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa
     1501 aggaaagaaa tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat
     1561 acaaacatta agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat
     1621 ttctctcatt tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc
     1681 caatcataaa aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc
     1741 ttcacttgcc ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt
     1801 gattgcactt gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc
     1861 tgaaattgac ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg
     1921 cttgctgaaa gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc
     1981 cttagtttta atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg
     2041 gacatttatt ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat
     2101 cagttctgcc ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa
     2161 acctcgatat tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt
     2221 atatcagtct gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga
     2281 acctgaaatc ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt
     2341 tgaattaaaa ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca
     2401 aatcgatatc tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga
     2461 agcaatgaaa agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt
     2521 gaataagcaa ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt
     2581 ggcagtcaag agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc
     2641 tatctctgat cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata
     2701 taaaggaatc caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag
     2761 tttgcgttac caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga
     2821 tagccagctc aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc
     2881 ttatcaaggc gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa
     2941 agcactgaat aaattaagcg gaaaaataac agaggatcaa atgattgata tgaattacca
     3001 agtcaatgtc gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg
     3061 attagtaaag gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag
     3121 tatgatggaa agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt
     3181 gttttggttg gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta
     3241 tctaaaccat cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg
     3301 cagaaaatgc ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg
     3361 acagtcaagc aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga
     3421 gcagaccgtg tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat
     3481 cgtatgccta gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt
     3541 gctgcaagcc cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca
     3601 agaaactcat tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt
     3661 ttcgttacgc acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag
     3721 gatggagaaa ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac
     3781 tacgttcggc atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta
     3841 gtcgtcaatg gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt
     3901 gatacgccta tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta
     3961 gaaaagcaaa gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga
     4021 gaggaggaag gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt
     4081 aagtgctgct tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct
     4141 atggggcggg acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt
     4201 agaggcacgt aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc
     4261 caactgggaa gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg
     4321 aaaaaaacaa ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga
     4381 tgattcatct attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac
     4441 cgggcaaaga cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga
     4501 ctttttatca ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc
     4561 ttttgagcta gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa
     4621 tcaacggcca ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga
     4681 agcatctggc attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac
     4741 tcgctatcgt ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac
     4801 tgggagacag ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa
     4861 acacggaatt gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg
     4921 agatattgtt tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta
     4981 tgtagctaag cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac
     5041 gatcgtctac gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc
     5101 gtcagatcaa gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga
     5161 accagttgct aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt
     5221 gattagcgag caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt
     5281 gactaaaaaa gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc
     5341 ctatttattg aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc
     5401 tacactttaa aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt
     5461 gtgaacttag acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac
     5521 gcttgaagcg ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa
     5581 gaaaggaggt aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt
     5641 aattatttgg cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc
     5701 tgctgctgta ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac
     5761 gacattaaat gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc
     5821 cttggcaatg gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc
     5881 acaaatgttg ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat
     5941 gcttccaaaa gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc
     6001 ggatacattg aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga
     6061 tacagggaag atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg
     6121 tttaaaagtg gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc
     6181 tcaagcaggc ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt
     6241 ttttgctgcg atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt
     6301 attgaaaaaa gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa
     6361 aaattatggc ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa
     6421 acaagtagca aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta
     6481 ccgaatacta atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct
     6541 ggtttaaatg tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt
     6601 gcggacaaag cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat
     6661 ggacagaata taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc
     6721 gccaccctat cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca
     6781 ggttggcaaa gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg
     6841 gacttttcaa aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa
     6901 tcctcagata tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt
     6961 attgaactaa actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg
     7021 gtaattatta cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg
     7081 gatgccacga aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg
     7141 ttgcaaaaat atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg
     7201 tcaaaaaatt atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc
     7261 aattatacag cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa
     7321 ctattcacta atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact
     7381 tgaatgtagt cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag
     7441 atacctttac atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt
     7501 taacaggagt ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg
     7561 gtctccatac agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg
     7621 tctatcaaaa cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag
     7681 taagggatta tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat
     7741 tatctggcgg gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt
     7801 acttgcttct tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag
     7861 actggatgtt gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata
     7921 tcgaagaagc aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg
     7981 tgattgctga gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat
     8041 cagagttgaa gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct
     8101 cattgtcatt tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg
     8161 acgatcatca attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt
     8221 aagactcaag ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa
     8281 caagttgagc cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt
     8341 tggacgaatg taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca
     8401 gccataaatg agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat
     8461 tatatccaag cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa
     8521 caagactata ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca
     8581 gcagtctggc aactagctaa agcagtccca atcgaccact tacttattga aacggatgga
     8641 ctacatgcag cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg
     8701 cttgaagaac agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa
     8761 aaaaatactt atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg
     8821 acacaactag tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta
     8881 ataaatgaat cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa
     8941 ccataacgtg aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca
     9001 ccatttaagt aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg
     9061 taagaaccgc ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga
     9121 gtagcagcag tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt
     9181 ttttccgttt gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga
     9241 acggctgctt gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt
     9301 tgtcctgagt a
//
LOCUS       DAZULG010000102         9037 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000102 DAZULG010000000
VERSION     DAZULG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.105"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..1505)
                     /gene="menA"
                     /locus_tag="ACG9CY_002257"
     CDS             complement(552..1505)
                     /gene="menA"
                     /locus_tag="ACG9CY_002257"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS4938748.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1518..2621)
                     /locus_tag="ACG9CY_002258"
     CDS             complement(1518..2621)
                     /locus_tag="ACG9CY_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS4938749.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2711..3646)
                     /gene="pplA"
                     /locus_tag="ACG9CY_002259"
     CDS             complement(2711..3646)
                     /gene="pplA"
                     /locus_tag="ACG9CY_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFS4938750.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3948..5882
                     /locus_tag="ACG9CY_002260"
     CDS             3948..5882
                     /locus_tag="ACG9CY_002260"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4938751.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            6339..6755
                     /locus_tag="ACG9CY_002261"
     CDS             6339..6755
                     /locus_tag="ACG9CY_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS4938752.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6769..7335
                     /locus_tag="ACG9CY_002262"
     CDS             6769..7335
                     /locus_tag="ACG9CY_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS4938753.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            7358..8344
                     /locus_tag="ACG9CY_002263"
     CDS             7358..8344
                     /locus_tag="ACG9CY_002263"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4938754.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8499..>9037)
                     /gene="scm"
                     /locus_tag="ACG9CY_002264"
     CDS             complement(8499..>9037)
                     /gene="scm"
                     /locus_tag="ACG9CY_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4938755.1"
                     /translation="DSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSS
                     TTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVI
                     LLVIGLRITFMSYKKSKR"
BASE COUNT         2686 a         1733 c         1824 g         2794 t
ORIGIN      
        1 agatgtttaa aaaagtgtga ctttgtataa ataaattaga agaagcaaac gaggatgtta
       61 aaagaatacc atatagttac caaaccaaga aagttctcgt caagttcgct ggtacgaatc
      121 aaatcaagtg aaaaaaggga ctgtgaaaaa aattgtgtca tagttagtca ctttttccga
      181 acaaatagtg ttcaatcagc tgtccttcgg tattagctca aaaaacggca aaatatgagg
      241 aactattttt ccatttgttt ctctaatact caggacaaaa cactgatttt acaacctctg
      301 ataaacggtg ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtgga
      361 aagccatttt cacaaaattt ttcttaatac tcgaaactaa gcgacttttt cacaacctcc
      421 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
      481 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      541 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      601 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      661 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      721 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      781 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      841 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      901 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      961 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
     1021 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
     1081 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
     1141 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
     1201 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
     1261 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
     1321 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
     1381 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1441 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1501 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1561 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1621 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1681 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1741 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1801 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1861 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1921 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1981 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     2041 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     2101 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     2161 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     2221 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     2281 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     2341 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     2401 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2461 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2521 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2581 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2641 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2701 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2761 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2821 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2881 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2941 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     3001 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     3061 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     3121 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     3181 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     3241 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     3301 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     3361 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3421 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3481 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3541 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3601 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3661 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3721 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3781 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3841 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3901 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3961 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     4021 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     4081 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     4141 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     4201 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     4261 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     4321 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     4381 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4441 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4501 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4561 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4621 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4681 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4741 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4801 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4861 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4921 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4981 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     5041 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     5101 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     5161 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     5221 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     5281 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     5341 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     5401 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5461 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5521 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5581 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5641 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5701 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5761 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5821 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5881 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5941 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     6001 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     6061 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     6121 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     6181 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     6241 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     6301 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     6361 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6421 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6481 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6541 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6601 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6661 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6721 cagatggagg tgacgaagac agtctgattt attagacctt tacacaccat gagtaaatta
     6781 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6841 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6901 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6961 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     7021 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     7081 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     7141 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     7201 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     7261 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     7321 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     7381 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7441 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7501 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7561 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7621 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7681 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7741 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7801 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7861 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7921 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7981 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     8041 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     8101 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     8161 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     8221 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     8281 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     8341 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     8401 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8461 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8521 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8581 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8641 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8701 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8761 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8821 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8881 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgtggt agaggtagta
     8941 tctgtactgt cacttgaagc tgatgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     9001 gtactgtcgc ttgaagctga cgtgtccgtg ctgtcac
//
LOCUS       DAZULG010000103         8827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000103 DAZULG010000000
VERSION     DAZULG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.106"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..786
                     /locus_tag="ACG9CY_002265"
                     /pseudo
     CDS             278..786
                     /locus_tag="ACG9CY_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            879..2041
                     /locus_tag="ACG9CY_002266"
     CDS             join(879..1155,1155..2041)
                     /locus_tag="ACG9CY_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4938756.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2321..3301
                     /locus_tag="ACG9CY_002267"
     CDS             2321..3301
                     /locus_tag="ACG9CY_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4938757.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3423..4841
                     /locus_tag="ACG9CY_002268"
     CDS             3423..4841
                     /locus_tag="ACG9CY_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4938758.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4912..6381
                     /locus_tag="ACG9CY_002269"
     CDS             4912..6381
                     /locus_tag="ACG9CY_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4938759.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6391..6858
                     /locus_tag="ACG9CY_002270"
     CDS             6391..6858
                     /locus_tag="ACG9CY_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4938760.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6894..7766
                     /gene="scrK"
                     /locus_tag="ACG9CY_002271"
     CDS             6894..7766
                     /gene="scrK"
                     /locus_tag="ACG9CY_002271"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4938761.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7847..>8827
                     /locus_tag="ACG9CY_002272"
     CDS             7847..>8827
                     /locus_tag="ACG9CY_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4938762.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDK"
BASE COUNT         3036 a         1316 c         1779 g         2696 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaggaaata agaaaactaa acttgtatcg
      601 ttaacaaaat atggtcgaaa ggtgtacgaa tcacataatt atttacatga agagctttat
      661 agctcaataa atgaagcgtt agaatcattc tcaccagacc aaatttcaac cattaaacag
      721 attctagaaa aagtatctta tacaatcaat gaatatcaag taagtctgac aagaaaggaa
      781 gattgacatg aaaaaaatga tttttgaatc ggctacacta gactagacag aaaaaataag
      841 gtgtgtaaaa taaatgaaaa cacactggag gaatttttat gtcaagacgt caacgtagaa
      901 cctattcaaa agaattcaaa caacaagtcg tcgatcttta tctcgcagga aagccccgag
      961 ctgaaatcat tcgagattat gagctgacta cctcggcctt tgataagtgg gtcaagcaag
     1021 cgcaaactac tggctcgttt caggaaaaag ataacttgat ccctgaacaa gtagaactaa
     1081 ttgcattacg aaagaaaaat aaacagcttg aaatggagaa tgacatttta aagcaagcgg
     1141 cgctgatatt gggacgaaaa gacaagtaat tgataccaat aagcacaaat actcaatatc
     1201 agcgatgtgt aaagtgttaa acatctcttg ccaaacctat tattatcaag ccaaaccagt
     1261 tgaagatgaa tcagaactgg aagaactcgt tcaagaagaa tttattcgca atcgaaaagc
     1321 ttatggtacc aggaagctga agcagtgctt ggccaatcta ggtcttcaag tgagtcaacg
     1381 cagaatcggc cgtatgatga aacagcgtgg cttgcgttca acatacacat tggctcattt
     1441 caaattccat tcttctactt gtaacgaagc caagaccgcc aatattctag acggaacctt
     1501 cctacaggaa aagtcattag aagcgattgt aaccgacctt acctatgttc gtgttgggaa
     1561 gaaatggcag tatatctgcc tgattcttga tttgtttaat cgagaaatca tcggctattc
     1621 atgcggggag aagaaagatg cgacgttagt caaggaagct tttgcaaaga ttccgtactc
     1681 gttgaataat gtacaacttt ttcacacgga tcgtggaaaa gaattcgata accagacgat
     1741 tgacgaaatt ttatgggcct ttaatatcac gcgttcctta agcaaaaaag gctgccccta
     1801 tgataacgcc gttgcagaat ccacctataa atctgtcaaa gtagaattcg ttcatcaata
     1861 ccgctttgag acattggcta atttgcgatt agagctattt gattacgtcc attggtggaa
     1921 taccatccgg ctgcacggca cgttaggcta tgagacaccg atcagttttc gacagcagag
     1981 attggcgcag cgaatccttg ataatgagct cggatgtgat accggtggcg aggcacctta
     2041 attatttgaa tgagcttctg ccttagagaa tcagattcga ggaggctcgt tgtcaagggc
     2101 agtcggaaca ccagcagcaa gcttacgcaa taccttataa tttttgtcaa aaaaagtgtt
     2161 gccattccac atatatcttt aaacagctat tgccagataa aaataaatga tattatatat
     2221 ataaatactt atccgaaaag gggaatgaga gagtcaaggt tcttggaatg tcgaatagtc
     2281 gagtaggtag attttctatc tacaaccttg ttgtttaaaa atgaaaaaaa ttggaattag
     2341 agaagttgcg aaagaggcaa atgtttctcc tacgactgtg tcgagggtat tgaatgatcg
     2401 tggatatttg agtgaggaaa caaaaagaaa agtttttgat gccatgaaaa aattagaata
     2461 ctatcctaat gaactggcga gagcactttt taaaaacgaa acatataccg taggtttgat
     2521 ttttcctaca attactaatc catttcacgc tgagttaatt caagatatcg agttcgagct
     2581 ttccagcagg ggatataaag tgttgctgtg caatagctta aacaatccaa caaaagaaaa
     2641 agagtacttg acgatgttga gaaagaatca ggttgatgga attatagtag gaacgcacaa
     2701 taaagatatt gcggattatg atattccagg gctacctatt gtagcgattg atagaagtct
     2761 tggtgaaaat acagttacag tatcttgtga taattacagg ggtggggaat tagctactca
     2821 actacttgta aatcatggct gtcagaatat actgtgtatt cggggagata gtaagataaa
     2881 gctacccgcg aatagtagga ctttagcata taaagatgtt ttgaataaat tcatgctgga
     2941 accgttagtc ctagaagtac catttgtaaa agaaatcaat gaaaaaaatg aaataattaa
     3001 gacatatctg gaaactcatc caaatattga cggggttttt gcaggagatg atttattggc
     3061 ttctttggcg attaacaact taggaagttc gggcaaaaga gttccaaaag aggttaaagt
     3121 gattggattt gatggagctc aacaaacatt gatttataat ccagaattat caactattca
     3181 acagcctata gatcaaatat ctaaagtagc agtaaaaaaa ttattgaata tgattaaggg
     3241 cgaaaaggag acggatgaac tttcacttcc agtaaagctc ataaaaaaaa atacagcata
     3301 agttaaatcg gcgaaaaatt ccttcgtcga tttaaaaata tttttatgaa acgggttgac
     3361 atatgaaacg ggttgacata tattagttgt gaagataaaa accttttcac tggaggaaga
     3421 aaatggagaa attattaatt aaaagcaata atcatcaact ttactaccaa cctaaggatg
     3481 tttgggtcgg agatgttatg ccttacggga aagaaggaaa attctttatt tatcatcaac
     3541 gtgatactag aaatccagtt ccttttggag aaccgtttgg ctgggcttta gcaactacga
     3601 aagattttgt tcattttgaa gactttgggg aatctctttt aagaggggat gacaatgaga
     3661 gagaccagtt tatctacgct ggttctgtat ttgaggcaaa tggaaaggtt cacgcatttt
     3721 atacagggta taatcgtgaa ttcttagcgc agggaaagac atctcaagtg ttgcttcatg
     3781 ctgagagtga tgattttgtt cattgggaaa aatcagaaac agcattggag ttagaaccac
     3841 aagaagggta tgatattcga aactggagag atccatttgt cgtttgggat gatgaaaaag
     3901 aagaatattt gctaatttta ggggcgcgaa agggtcagga taaacatcta caaaccggac
     3961 gtttagtgga atttacatca aaagatttaa ctaattggga attcaaaggt gatttttggg
     4021 ctccgggatt gtataccatg tttgaaatgc cagaactttt caagattggt gactggtggt
     4081 atcttgtcta ttcagaatac tcagatggaa ataaaatact ctatcgaatg agtcaaagtt
     4141 tggaaggtcc ttggattaag cctaaagatg aagcttttga tggacgggct tattatgcag
     4201 gtagaacagc ttatgatgga aaacgtcgag tcctatttgg atgggtacca acaaaagatc
     4261 aagaaaatga taaaaacaat tatttatggg gaggtacttt ggttcctcat gaagtctatc
     4321 aaaaagagga tttttcactt ggggtaaaac ccatagacga agtgtggaat gcattcataa
     4381 atttacatga tattgccgat gtagagatag aaacagcaga cacaagagaa gaagtaacgc
     4441 ttgtcaaaaa tagtggaact ttgtttaatt tagaaacaac aatttcattt gatgagaatc
     4501 ttagtgactt ttcactgaga ttatataaaa accttgagac agatgaatct tacgaatatc
     4561 gcttcaattt agatgagggg cgtcttacat ttgataaaaa cccttgttac ccttggtttc
     4621 aatatatgga taaaggactt attagaccta tatctttaaa gcctaaccaa tcttataaac
     4681 taaaagtgat tgttgatgac aatataatga cactttatat tgatggaatc gctcttaacg
     4741 ctcgtgtaca tggttgtttt ggtcaagcaa ttactattac agcaacaaat ggatcaatga
     4801 ctgcaataaa taccaagttt tcaaatgaaa taaagaaata ataaacgtca atcatgtggc
     4861 aggtctgaaa atcaaacatg acctgtcaca tttaaaaggg aggataaatg aatggactac
     4921 aaaaaaattg ccgaagatgt attggaaaac gttggaggaa aagagaatat ctcaagcgct
     4981 acacactgca ttactcgttt acgtttaatt cttaaagaca aaagcaaagc gaatatggaa
     5041 atgcttgagg aaatagaagg tgcaaaaggt gttcttttca atagtggaca gttacaagta
     5101 attttcggaa caggtataat tgaaaaggtt tacaaagagt tcgttgaatt gacaggagct
     5161 aaggaagtat ccgtttctga aatgaaagac gaaggagtca ataattcagg aaatagactt
     5221 caacgagctt ttaaagtttt ctcggatatc tttattccga tcattccagc ttttattggg
     5281 gcagcaatgg ttttaggaat tcgttcgttg attaccactc ctggaatgtt tggaatggat
     5341 ggctcattcg ctgatcacaa tgtattccta aatgatttat ccaaatttct gggtattatt
     5401 gcaaccacat ttgattattt accagtttta gttatgtatt ctgcaacaaa acgttttggc
     5461 ggaaacccta ttttaggatt acttgtagga tttgttatga ttcatccagg actagccaat
     5521 cggaatgatt tcgtcatggg aactataaag cctgattatt ggcatttatt tggattaagc
     5581 attccagcag ttgcgtttca agggggtgtt tttccagcaa ttttgacttc ttggttttta
     5641 gcaaaagttg agaaaattac aaataaatat tctcctcaag tacttgctta cattttagtc
     5701 ccaactgtga caattgttct tgccaacttt gcactatttt tagtgtttgg tccattgggg
     5761 aattttatcg gtaatgtttt aggtggaatc attgatttcc tatatatgaa gatgggtgcc
     5821 tttggtgctt ttgtttttgc aggcgcgtta caaccattag ttgtaacagg aacacaacat
     5881 gctattcaag gtattgaggc aaacttagtc gcaacaactg gattcaatta tatccaacct
     5941 atttggtcag tttcaattat tgcccaaggt ggcggttgta ttggtatgta tttactcttt
     6001 aagaaaaaat ctaaagatag agatattgcg atttcaagct ttattccaac actggtaggt
     6061 attactgaac cggctatctt tgctgttaat ttgaaatatt caatcgttcc ttttgtttgc
     6121 tcgtttattg gtgctggttt tggtggagca tttatgaaaa tttttgatgt aaaagccatt
     6181 ggacaaggat tgactgtatt gccaggtctg actattgcta atccagttct tccttatata
     6241 tttggtaatc tcatagcatt cattttacca atcattttta ttctcgctta tgataaatat
     6301 aaagtgttta tccctgaagc taaacaccgt aaatttgcag aaaaaactga ctcaaaaaaa
     6361 ttgccagttc aagtaaagtg agatgagagc atgttaaatt tttttagaaa aaatgaaata
     6421 agcgttgtag cacctgcaac cggtgttttt gaaaaactgg agaaattaag tgatccagtc
     6481 ttttcaaaag gaatgatggg acaaggattt gcaattgatg caaaaaacga aacgattgta
     6541 tctccaatcg atggttttgt ttcaagtatt tttcctacaa aacatgcctt aggaataaaa
     6601 acaaaatcag gaattgagat acttgttcat gtaggaatag atacagttga gctaaatggt
     6661 gagggatttg atattaaagt tcaagaagga caaaaagtta aggctgggga tattcttatg
     6721 accattaatt ttgtagtttt tgaaaagaat cacaagacta aagatgtcat tgttgttttt
     6781 ccagactacc aagacgaaat tttcattccg aaacttgaag aagtaaaaca aggacaagaa
     6841 ttgtttgttc ttggctaatg ataagtaatt aataatagaa tcgaggaaaa tgtatggttt
     6901 ttggggctat tgaagctggt ggaacaaaat ttgtttgtgc cattggagat aaagatttga
     6961 acatatttga aaaagtcagc tttcccacga ctacacccaa agaaacaatg aatttagttt
     7021 tagatttttt taatcatcat aaagatgatt tagaaggtat cggagtggga tcgttcggtc
     7081 ctattgatat tcattgtgaa tctaaaacgt atggcttcat taaatcaacg ccaaaacttg
     7141 cttggcagga ttttgatttt attggaagtt taaaaaagca acttaatatt ccaactgctt
     7201 ggacgactga tgtaaatgcc tcttgctatg gtgaatatgt ttctggaaat ggcagaggcc
     7261 tttcaagtgt tgtttactat accgtaggaa cgggcatagg aggtggcgct atccaaaacg
     7321 gaaacttcat tgaaggtttt agtcatccag aaatgggaca tatgcttgta aaacgacaca
     7381 ataaagacaa cttttctggg acatgtccat tccacggaga ctgtttagag ggcttagctg
     7441 ctggtccggc aatcgaaaga agatcaggga aaaagggaca agaagtgact gaaaatgacg
     7501 ttacttggga aatagaagct tattacattg ctcagtgtgc atataataca acattgatgt
     7561 tatcacctga tgtcattata tttggtggcg gagtaatgaa acaaaaacac ttaaaaaaga
     7621 aagtacaaga taagtttatt gagttgatca acggatatgt cgctattccc gatgttgaca
     7681 attatattat gacaccaaaa ttgttagata attctggtat tgtaggttgt ttagctttag
     7741 caagagacgt taaagaaaaa tcttaatttg gaaaaaagat cgtctcgact atttgtttgg
     7801 ttgatgcgat ctttttttca attagtaatt gaagggacgc ctaaaagtga aaaaaaaaat
     7861 gaaagatgaa gtattagagt atatcaaaaa taatttaaag tattacgatt tttctgctca
     7921 agatattgca atgaaatttt gtattaaaag aaatgtcgct tcccactatt tgaatcagtt
     7981 attttcagat ggaaaattac taaagaatga ttctgttaga ccagtaatgt tcaagtataa
     8041 tcaacaaaag ccaaaagact gcttttctaa gtttattgga gcagacatat ctttgaaatc
     8101 cactatagat aaatgcaaag ctactgtaat gtatccccct aatggcctcc cattaataat
     8161 aaaaggaaac agtggcgttg gaaaaagttt tttagcaagt ttgatctatc agtatgctct
     8221 tgatagaaaa gtaattcata atgacgcaaa atttgttgtt gtgaattgtg cagattatgc
     8281 aaataatcca gagctattgt cagcagtttt atttggatat aaaaaaggag cctttactgg
     8341 ggctgaaaaa gatactgcag ggatgctatc ttctgctaat ggaggatacc tttttttaga
     8401 tgaggttcac aatctaagtg cagaaaacca agagaaattg tttcttctta tggattctca
     8461 aaagtatcgt aaacttggtg aaagtgtgca atgggagtat gcaaatgttc gccttatctt
     8521 agccacaaca gaagataaaa atacctcttt gttagcaact tttagaagac gcatcccagc
     8581 tgaaatcact ctgccagatt acaataatag aagtacatct gaaaaaattc aattattatt
     8641 tgaatttctt aaagatgaag caattacgat tgattcaaaa atattttgtt cgattgaact
     8701 ttttactgat cttttatcga aaacatttga aggaaatgtt ggagaattga ggaatgaaat
     8761 caagcttctt tgtgcagaag gttatttagg aaataagaga aagtcttcaa tttatttagg
     8821 ggacaaa
//
LOCUS       DAZULG010000104         8731 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000104 DAZULG010000000
VERSION     DAZULG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.107"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG9CY_002273"
     tRNA            2..74
                     /locus_tag="ACG9CY_002273"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG9CY_002274"
     tRNA            78..150
                     /locus_tag="ACG9CY_002274"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG9CY_002275"
     tRNA            155..225
                     /locus_tag="ACG9CY_002275"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG9CY_002276"
     tRNA            238..311
                     /locus_tag="ACG9CY_002276"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG9CY_002277"
     tRNA            323..394
                     /locus_tag="ACG9CY_002277"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG9CY_002278"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG9CY_002278"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS4938763.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG9CY_002279"
     CDS             1983..3455
                     /locus_tag="ACG9CY_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4938764.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG9CY_002280"
     CDS             3538..4326
                     /locus_tag="ACG9CY_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4938765.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG9CY_002281"
     CDS             complement(4393..5061)
                     /locus_tag="ACG9CY_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS4938766.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG9CY_002282"
     CDS             5176..6888
                     /locus_tag="ACG9CY_002282"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938767.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACG9CY_002283"
     CDS             6891..8663
                     /locus_tag="ACG9CY_002283"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938768.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2622 a         1487 c         1944 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc a
//
LOCUS       DAZULG010000105         8603 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000105 DAZULG010000000
VERSION     DAZULG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.108"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(201..2582)
                     /locus_tag="ACG9CY_002284"
     CDS             complement(201..2582)
                     /locus_tag="ACG9CY_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4938769.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2572..3219)
                     /gene="recU"
                     /locus_tag="ACG9CY_002285"
     CDS             complement(2572..3219)
                     /gene="recU"
                     /locus_tag="ACG9CY_002285"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS4938770.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3283..3822
                     /locus_tag="ACG9CY_002286"
     CDS             3283..3822
                     /locus_tag="ACG9CY_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS4938771.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3912..4331
                     /gene="gpsB"
                     /locus_tag="ACG9CY_002287"
     CDS             3912..4331
                     /gene="gpsB"
                     /locus_tag="ACG9CY_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS4938772.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4353..4720
                     /gene="rnpB"
                     /locus_tag="ACG9CY_002288"
     ncRNA           4353..4720
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG9CY_002288"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4929..6095
                     /locus_tag="ACG9CY_002289"
     CDS             4929..6095
                     /locus_tag="ACG9CY_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS4938773.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6141..7637
                     /locus_tag="ACG9CY_002290"
     CDS             6141..7637
                     /locus_tag="ACG9CY_002290"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS4938774.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7718..8401
                     /locus_tag="ACG9CY_002291"
     CDS             7718..8401
                     /locus_tag="ACG9CY_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4938775.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8410..>8603
                     /locus_tag="ACG9CY_002292"
     CDS             8410..>8603
                     /locus_tag="ACG9CY_002292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938776.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2699 a         1565 c         1830 g         2509 t
ORIGIN      
        1 gtaaatggat aaagtatatg taatgataaa agaaaaatgg attttctaat ttttaagcta
       61 aatgttagat cacacatgta tatgctggat agtcttataa aaaatcatgt aatacagctt
      121 ggtacatgat ttttatttgt agctgttttt tacatagaca caaaaaagaa accacgcttg
      181 tagacgtggt ttctttttta ctatctgttt ccagaagctg aggtgctgct ggatggtgta
      241 tggtcatttg cagcatccgc tccgctagtt gctggctcac tggaagaagc aggaggagca
      301 gatgaagacg gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt tgaagacgct
      361 ggctcgcttg tttctgctgt cgtgctttct tttgaggact cagcagttga ttcgctttga
      421 ctagtagagc tagagctttg agtcgttgtc tctgttgttg agcttccagg agtctggaca
      481 tagctggaag atggaatagt tgttgatggt gtaattgcat tcgatctagc tgtatattga
      541 tctttgtagt acagttcacc gcccacacga attaatccgc ttggcatctc ccagtctgta
      601 tttgtcacgt tagctgaaac atactgcata agctcgcgat aaacatcaga ggctacgtgt
      661 gacgattctg aagtgacagg cgtcattttt ttattgtaac ctgtccacac ggaaatcgaa
      721 taatttggcg tatagcccgc aaataggata tctggataca ccccagaaga aataccaagt
      781 tttgcatact cgtcatctgt gtaattggat gttccggtct taccggcttg atataaccca
      841 gcgatttgcg cattcgtacc agttccttca gtgatcgtat cttttaaaat gtctgtgatc
      901 atataagctg tttctggaga catggctgtt tttccatctg gctcataagt ttcttctgtt
      961 ccgtcttgga agacgatttt attaacatat tgaggtttgt aataagtccc tccattggcg
     1021 aaagccgcat aggctgctgc catttttaag gaagaagctc cgtattttgt cccatcttgt
     1081 tcttccgtat tactagagat cgcattcgat tgatggatcg tactgtattc gatcccaaga
     1141 ttttttaaga aagaagccac tttatctgaa ccgacttcat tgaataattt gactgctgga
     1201 acattacggg ataagtaaag tgcttggcgt aaagtaatgg tgcccatata ttgattatcc
     1261 cagtttccta caggagtcga agtcccttca tagttatacg gtgcgtcagt aatagttttt
     1321 ccagtagaat atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg tttcatcgta
     1381 gaaccaaaat cacgggaagt attcactgct aagttgttgc ctaaagttac gtcttctgcg
     1441 atgtgacgtc cgccaatctg ggcttttact ttcccggtat tggtgtcgat aagggtggag
     1501 gcaacttgca tctcgtcgtc tgggtaagag acatactgat ccgtgttgac gatatcatat
     1561 agtttctttt gggcgtccag atccaaattc gtataaatct ctaaaccgtc tgtgtagaca
     1621 ttcttatctg ttttttcttg tacttcattg atgacttctt tgacataatt atcgacgatc
     1681 ttagtattgt catcggattg agttagttct tgcagaccat ccgttaccgg tacattaaca
     1741 gcttgatcat attctgtttg agagattttt tcgttttgaa gcatcgtata caaaacagta
     1801 tctcgtcttt tcttcgcctg atctggataa acataaggat cataggcgga aggggcttga
     1861 ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt ctgataattt cttaccaaag
     1921 tacatctcag aagctgtctc catcccgtac aagccgtttg acatatacac tttatttacg
     1981 tagtaggtca agatctcttg tttcgatttt ttttgttcca atcttacagc catccacgct
     2041 tcttgggcct tgcgtttcag agtctgatcc tcggcactgg tggagaagaa ggaaagtttg
     2101 atcaattgct gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa gttagagagg
     2161 gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg gcgatcttct
     2221 accgacacga tcgcatcttc taatgttttc ggtaattcat tcgcagaaat tttttcacgc
     2281 ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg tatatagttt ggaagatacg
     2341 gtagcgtcta attttttatc cgtcaattcg ggcgcatctt ttgcgtaata ccagaaaagt
     2401 cctacacctg ctaaaaagag gatacagaaa aatgagagta ttcctaaaag gatcttgata
     2461 aataatcctt ttttatgtgt tccagaggac tttccagaac ctttgccaga attcttcttc
     2521 acactctttt taggagtagg ctgtttctgt cgtctagagc ttctagtttg ttcatttgcc
     2581 attcttgctt tctccttcaa tagataatgt tgataaatag aagtcaaccg cgtccaaata
     2641 aggaatccgc ggagaaatac cagttttgat ctctattcct agttcttcga tcaaagataa
     2701 aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag taagcaatca aatgaacact
     2761 attcaaataa aaacagcgct tcaaagacga aaaccataac aagacaaagc agaccccatt
     2821 ttgttcgagg catgctttca tatgttcgat ttgatgggca tggaagtttt taaagggaaa
     2881 agatgttttg ttcttggttt cttttgcttc aaagtccaag taaaagcctc gatagacgcc
     2941 attatagtct gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag cagcacttct
     3001 cttaggatag tctactttca cgatttggat aggggtaggc tttttatgga taaccgccaa
     3061 cccgcgggaa agataatatt tattactttc attgattgct tcttcaaagc gcatcccccg
     3121 attgccgaat tcagtgatct gatttctttt ttcttgaatt ttttcgacgg gagaggcatt
     3181 cttataataa ggttggccat ttggatagcg caataccatc tttaatcaca ctcctaggcc
     3241 acattatagc aaaaaagcgg atagaaagaa aaggaatctg agatggataa gtcaaaagta
     3301 atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa tgatcctaaa
     3361 atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga agaagggctg
     3421 gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca aatagtagcg
     3481 gaattgaaag aagaatatcc ggaactatct cttggtattt tgtatccttt ttctgatttt
     3541 ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca attagcagat
     3601 tatgtcgaag ctgttagcca tcaaccctac caatcaccta gtcagctaaa gaatcacact
     3661 cgttttatac ttgagcatac agtgggctgt ctgctgatct atgatgaaga attccctgga
     3721 aaaacgaagt atttttttga agatgcacag aattaccaag cagatcatcc ctacgaaatt
     3781 cggctgatca gtatggacga tcttcaaaat tttagtgaat aatcgatatt gtgtttgatt
     3841 tatcaacttt tttcctatac aatgaaaaag gtagaaatgt tattacgtaa atgaaaacta
     3901 gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga cattttacaa caagaattca
     3961 aaacaaaaat gcgcggctat gatcccgttg aagttgatga attcttagac aatatcatca
     4021 aagattatga aacttacagc aaagaactcc ttgctttaca agaagaaaac gatcgattga
     4081 gtgcaaaagt agctcaatta tctaaaactc aaggagcagc tcaaacacgc gtgcaacaaa
     4141 cagaggcacc aaagagcgca gcagtgacca attttgatat cttaaaacgc ttgtcaaatc
     4201 ttgaacgtga agtatttggt aaaaagcttg atcaacaagc aagtgcagtc aaaccagcac
     4261 aacctaatcc aaataactac acaaatgcag acacaagctt agatgataat gaaaaaacac
     4321 gtcaatttta atttttgatt aatgaatttg cggttttcgg gtaatcgcgg tctgacttga
     4381 tcaggctgag gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt cgtgcttagc
     4441 gaaaccataa gctaaggtac tctttggagt aacggcagga aaacaagcta aggcttcggc
     4501 tatgcttaag tatccttgaa agtgccacag tgacgaagcg gctggggaaa cccatccggt
     4561 ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc tttttgtctg
     4621 gaagaagaac aggacaaaaa ggcagtataa ctgcagatag atggttaccg ctttgttttt
     4681 tctccctgga aaagcaaagt acagaacatg gcttatagaa aaccgcaaac atcagggaat
     4741 taggtcgtga caaaagtaat gcgttataag aaacaagaaa aacatttgaa agctattttt
     4801 caatacttca gatgaatcgt tcttattaca aaacaaactt ttgtatcacc ggttattcgg
     4861 aatttgagta aaagtgtggg gtaggactga tgtcccacct ccttttttta taaataagga
     4921 gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc aactgcggca agcgggctag
     4981 aagcactggt tggtaaagaa ttaagagatc ttggaatcga atgccaagtt gagaacggac
     5041 gtgcacgttt tgaaggaaca atggaaacga tcgctacagc taatttatgg ttacgtacag
     5101 cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat gaattgtttg
     5161 aaaacgtaaa agcattgcct tgggaagatt ttcttccact agatgcagct tttcctgtag
     5221 ctggaaaatc gatcaagtcg aaactatata gcacacctga ttgccaagcg atcacaaaaa
     5281 aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc agcttctgta ccactgacgg
     5341 aaactggcgc acattttcag ttggaagtag ctttgctgaa agaccatgtg atggtaactc
     5401 tggatacgac aggaccaagt ttattcaaac gtggttatcg cttagaaaaa ggtggagcac
     5461 cattgaaaga aaacatggca gcagcactta ttatgttgac aaattggcga aaagaccgtc
     5521 cattttatga tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc ttaataggcc
     5581 ataatatcgc accaggattc aatcgtgagt ttgcttgcga atcttgggat tggttcagtc
     5641 aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat gatattgaat
     5701 tagatattac tggttcagat atcaatggac ggatgattga aattgccaaa gccaatgcag
     5761 aagaaatcgg attaggctct tcgattacct ttaagcaaca agcagtcaaa gacttcaaaa
     5821 cggataaaga atacggggtc atcgtggcta atccgcctta cggagaacgt ctaggagaag
     5881 aagaaactgt tcggaaatta tataaagaaa tgggcgatgt attccgtcca ttaaaaacat
     5941 ggagtaaata tatcttgact agcgacttag cgtttgagga attttacgga caaaaagcaa
     6001 ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga tctattccag tattggggaa
     6061 ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta ctaattgatt cattcaattg
     6121 gatgtttgga ggaataggga atgaaagaaa aagagttttt acaagaagta aaagaaatca
     6181 atcttctaca acaagcttta ggaatcctcg attgggatac acaaacaggt atgcctgaaa
     6241 aagctagtgc ataccgaagt gaagtagaca gctatcttta cagcatttat ttttcaaaaa
     6301 aaatcggtcc aaaaatcaaa gaagccattc gctatttttc tgaacatccg gaagaattgt
     6361 cggaagttgg aactgccgtt tttgccaaag taaaagaaga gtatgatttg gaacatcgcg
     6421 taccggaaga tcttatgaga aaatatacag ctgccgtttc ttcagcacac ggacaatggc
     6481 aaaaagctag agaaacacaa caattttctg attttcaaca cgcattgaat caaaatattg
     6541 aattaacaaa acagttgata ccttactgga gaaaagatga aaagacgaat tatgatgttt
     6601 tgctgaatca atacgaaccc gggatgactg tcgaaatcct tgataaggta tttgatcagc
     6661 ttaaagaagg aattctttcg atccgccgta cgctccaaga aaaagggact gaacctgaaa
     6721 cagacttttt aaatcggaga atgacgaaaa aacaacagaa gagatttgta gtgaaagtaa
     6781 tcgaacaact tggttatgat tttagtcgtg gtcgtttaga tgacactgtc cacccattta
     6841 tgactggaat taatccgaat gatgcacgaa tcacgacacg atggaacgaa acagatttca
     6901 aaatggctgt ttttggagtg atccacgagg ctggtcatgg tatgtatgaa caagacatcg
     6961 atgataaata tgcgtataca ccggtttatg aaggtacatc aatgggtatc catgaatcac
     7021 aatcgttgtt taacgagatc attattggaa gtagtcgcag cttctggaaa aagcaatatc
     7081 ctttctttca agaatgtgca gaaggaacat tcgatgatat tccttttgac aaattttatc
     7141 gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt ttgacttacc
     7201 ctctccatat catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa ggggtagatg
     7261 ttgctgaact acctgaaata tggaatcaaa aatatgaaga gtatctggga atacgaccaa
     7321 caaatgatgt ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc tttggttatt
     7381 ttccctctta tgcattaggg tacatgtatg cggcacaatt acttcattct ttgcagcaag
     7441 aaatagatgt ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa aactggatgt
     7501 cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc atggatgcaa
     7561 cacatgaacc gcttaatccg caatacctga tcgactatat gaaagacctg tattttaatg
     7621 tatatcaggt aaaataaaat acaatttaca aagatagctg aaattcatct aatctataga
     7681 tgttttcagc tatttactta agaaagaagt ggaacgaatg gataaattac aagtgatcgc
     7741 tgattatagt atggaccaat tagcgcatga tcaaacagga catgggagcg atcataccaa
     7801 aagagttgtc aagttagctg aaaaaatttt ggagacggaa ccgcaggcag accgctttgt
     7861 gacgcttgca gcagcttacc tacatgatac gattgatgat aaagtcgtta aaaatgagaa
     7921 cgaggcaaag caacagcttc gtgtattcct tcgctctttg ccaatagtag aggaacagat
     7981 ctcgatgatt ttttccatta tcgaaaatat gtccttttct aaaaatctct ctgaagcggc
     8041 cgaactatct ctggaaggta agatcgttca agacgctgat cgtatcgaag cattaggagc
     8101 aatcggcatt ttgcgtactg cttatttcgg cggtggacat gggcacccga tttttgatcc
     8161 tgaactgtat ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca cgacagtgat
     8221 caatcatttt tatgaaaaac tgttcttgct gtctgataaa atgaatactg attacggata
     8281 tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc ttagaagaat tttttctgga
     8341 atggtctatt gacgatgaaa attttactcc agacaattgg atcaaaaata acaaaaaatg
     8401 atggaggttg tgacagaagt ggtcagctcc aagatatttc ggatcatttt ccgtaaaagt
     8461 tacttctgga acactgttta ccaagaggtc gtgagcctgc cgttcagctc cgagtcacaa
     8521 ggaacaattt caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg tgattcggaa
     8581 ttggcaggag aacaccgttt att
//
LOCUS       DAZULG010000106         8493 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000106 DAZULG010000000
VERSION     DAZULG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.109"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG9CY_002293"
     CDS             complement(<1..149)
                     /locus_tag="ACG9CY_002293"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4938777.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG9CY_002294"
     CDS             complement(593..1714)
                     /locus_tag="ACG9CY_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFS4938778.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG9CY_002295"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG9CY_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFS4938779.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG9CY_002296"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG9CY_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFS4938780.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG9CY_002297"
     CDS             complement(4304..5215)
                     /locus_tag="ACG9CY_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4938781.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG9CY_002298"
     CDS             complement(5232..6239)
                     /locus_tag="ACG9CY_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS4938782.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSIPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG9CY_002299"
     CDS             complement(6236..8365)
                     /locus_tag="ACG9CY_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4938783.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG9CY_002300"
     CDS             complement(8370..8480)
                     /locus_tag="ACG9CY_002300"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938784.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1727 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggaattga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DAZULG010000107         8416 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000107 DAZULG010000000
VERSION     DAZULG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.110"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            512..1075
                     /locus_tag="ACG9CY_002301"
     CDS             512..1075
                     /locus_tag="ACG9CY_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938785.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1136..1501)
                     /locus_tag="ACG9CY_002302"
     CDS             complement(1136..1501)
                     /locus_tag="ACG9CY_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS4938786.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1577..1822)
                     /locus_tag="ACG9CY_002303"
     CDS             complement(1577..1822)
                     /locus_tag="ACG9CY_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4938787.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1892..2146)
                     /locus_tag="ACG9CY_002304"
     CDS             complement(1892..2146)
                     /locus_tag="ACG9CY_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938788.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2310..2633)
                     /locus_tag="ACG9CY_002305"
     CDS             complement(2310..2633)
                     /locus_tag="ACG9CY_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938789.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2820..2893
                     /locus_tag="ACG9CY_002306"
     tRNA            2820..2893
                     /locus_tag="ACG9CY_002306"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2854..2856,aa:Ile,seq:cat)
     gene            complement(3278..3775)
                     /locus_tag="ACG9CY_002307"
     CDS             complement(3278..3775)
                     /locus_tag="ACG9CY_002307"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS4938790.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3815..4624)
                     /locus_tag="ACG9CY_002308"
     CDS             complement(3815..4624)
                     /locus_tag="ACG9CY_002308"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4938791.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4958..5815
                     /locus_tag="ACG9CY_002309"
     CDS             4958..5815
                     /locus_tag="ACG9CY_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4938792.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5808..6710
                     /locus_tag="ACG9CY_002310"
     CDS             5808..6710
                     /locus_tag="ACG9CY_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4938793.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6743..7093
                     /locus_tag="ACG9CY_002311"
     CDS             6743..7093
                     /locus_tag="ACG9CY_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4938794.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7142..8065
                     /locus_tag="ACG9CY_002312"
     CDS             7142..8065
                     /locus_tag="ACG9CY_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4938795.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8143..>8416
                     /locus_tag="ACG9CY_002313"
     CDS             8143..>8416
                     /locus_tag="ACG9CY_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent dehydrogenase"
                     /protein_id="HFS4938796.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAE"
BASE COUNT         2955 a         1395 c         1555 g         2511 t
ORIGIN      
        1 aaattcattg gtcgttgaat gggctgaata gtagtgtata aaacaatttt gtggagcgtg
       61 aagcttttat ggtagaggaa aatacgaata aagtgatgtt gataaaaaga ttcctatctt
      121 gaatatgaat cgagatgtct tgaaaatgaa tgagtttgac caatcattat cctatcgaaa
      181 tttacagttt cgttcgattg gtctgtcaat taatgaaaaa gtaaaaaaga atatgttcta
      241 ggaagagggc ggcacatcta aatttttaag taacggacag ttcaaatatc aagcattgaa
      301 agaggctatc ctactgtttg aaggaatacg aatatataaa aacgacaaag aggaaaaagt
      361 agttcattaa atcccttcaa gtgatgaaag aatgagatct aataaaacct gatctttcat
      421 ctgttttttt aataacgtaa taattttttt attttttgtg ctttcgccgt tattgtagag
      481 tgatttagca ttcttatttt taaaaataac tgtgttatat attaaaaaag gcataatagt
      541 tataagcttt tttaaagaat atttagaaat tattaatttt cgtctgaaaa caaaaaaaga
      601 aagcgttatt attttagtct attttcaggg ggggaacctt atgataaaaa aactattttt
      661 actatcaata ttaacgctag caatggcagg ttgttctgac tctggcttag caactgaact
      721 gatcacaaaa tcaactgaag ccacgacaga ggagttaaaa tgtggaaacg atcacagtga
      781 ctacagaggc ttaaatcgac gatgcatggg aatattaggc agaagcaatt gtaagcatcg
      841 atgtagtaat cgaagaaggt gtagaagaag caacagaaaa atcaaaagaa agatcaaaag
      901 aagcaatctg tgctgtgcat cgtatacaga cgtcgaagat gcggtacttt taaaccacgt
      961 caggagatgt tcatttatgt ctagggaagt tggcgatgaa ccaaaactag agtacgcttt
     1021 tagttttgca cgtgaggatg aagataaccc taaaacttac gacattttag cttgatctaa
     1081 gatggttaaa gctttttaat gaagtaagag gagatagaag gcttttctag ttatattagg
     1141 taagataaat tcctttttgc tccaaatttg ctaaacattc tgctagataa acttcatcgt
     1201 gttccggata aatcggcacg ttgcgctcta cagtaggtaa atcaaaaaat gcttcgcatg
     1261 atttgccacg atacatagga tttttccact ctggtgctgg gctgtaacca cgtttttcca
     1321 tttcatccat gacaagctga tgatattgga ataatctata aggagaatag tgaaaaacat
     1381 aatccacagt agcatgtttc tttccccagc catttcctct taatgcacag cattcccgat
     1441 gttgtcctaa aagctgttga cgaggaagct ttgaaatcaa tttttcatgc cataatcgca
     1501 tagaaatgtc tcctttatat atagattgtt aaccttagac gttaaatgaa aaaaccagca
     1561 ccaaggctgg aaaaaatcac ttcagtttgc ttgctgtttc tgagcgacca attaggtaat
     1621 caacggaaat atcgtaaaag tcagcgatat agattaattc aatgatgctt ggaatttctt
     1681 ctccttcttc gtacttacga tatttgaata aaggaatact taacgcgcta gaaacagcta
     1741 cttgagataa agatcggcca gttcttaaga aattcagacg acaacgtaaa gtatccagta
     1801 aaatagcttc ttcgatactc atttaatcac ctttcattat agtagaatag ttataatgac
     1861 tatagaaagc tacacatgca atcaatagta ctcaatgttt tttatttaaa cgtatcaaat
     1921 aaagaatgac gatcaataaa gacaaaacca gtcctgataa ttcttctata aaaacactat
     1981 cgggttcaaa gtagttgtaa ttaaaaactt tccacataaa gtagatagcc ggaagagtca
     2041 aaatagctaa ataaaaccgt tctttgttcc aattcataag ataaacaata cttgcgacca
     2101 gtgcgaccgc aggcacccaa aacaaaactc tcaccaacgt aatcatgtaa cggactcctc
     2161 aacgattcat ataataaaag ataacatgtt attgcataca tcacaaatga aatgctagga
     2221 aaataaaaga agaatagatc aatgaaaaat gaaaaagcag aacagttttc tgctgataag
     2281 caggaactgt tccgctgaat gtgtcttatc tatcgagaaa aaatcaatgg attgatatct
     2341 aagtaattca atacatataa aaagaggacg atcgcattca ctaaaaagga aagaatgatg
     2401 atcccatagc gaatggaaac atatttaaca aatacttttg tttgaatacg ggcatatctt
     2461 tttcctttat agctgagcat catgacaaga agcaaaaggg taatcacacc ttgaaaaatc
     2521 attccaataa acagaattcc tgaggtttga taatttaatt ggagaaaata agctgctgcc
     2581 gcaactaaat cgaataaaat cgcaataaat ggcaaaataa tcactcctaa cattgagaat
     2641 aaataaaaga ataacatttt ttctaaaata agcaaataaa aattaaagta acaaaatagt
     2701 tatgacacaa gtaaaagaaa taagaataac aatattattt tacttatatt ttgaaacttg
     2761 tgagagtttg tcattcgtgc tatagttgaa aagtaacaaa aataaatttt atttatgatg
     2821 tacctttagc tcagttggtt agagcagacg gctcataacc gtccggtcgt aggttcgagt
     2881 cctacaaggt acataagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
     2941 aagtaaaaac agcttcttat gtttttactt ggctgttctt attactgcag atgcaagctt
     3001 ttgaataccg tttaaggaag aggttgtgaa atcagcgttt tgacctgagt attagcttaa
     3061 caaacggaaa aatagttcct catattttgc cgtttgttaa gctaataccg agggtcagct
     3121 gattgaacac catttaagaa agaggtcgtg agcctgccgt ttcactctgt gtcataaaaa
     3181 agccaatcta ctttgataga aaaagcacgt aggactaaaa cagttctgcg tgcttttttt
     3241 attgtgtgtt aaatatcgta aatgaagata gatattttta tgattcgacg atagagacgt
     3301 tgaaatcttt caacagctct aaagcggatt ctccgtgttt tctactggag actaacattg
     3361 gatcaacaaa aaagtctgct tcaggaaaag cactctgcag gcctatcaga ttagagagta
     3421 cacacaagtt tgtttcaatc ccgactactt cgatctcttt ttgttcttct ttgctttcaa
     3481 ataaagtttc ctggatttca gctaaagttt caggtggaat cgtaaagtaa tactttttaa
     3541 tcactcgttc attttcttgt ggagaaagaa gagggatcaa ttgcaaatct tctcgttctt
     3601 cttctttgtc cttcagttca actggtatat cccgtgtgaa gagaatatat tcattatttt
     3661 ggcgtgcttt agctaatctt tgagcaatac gttctgccaa ttgatctgac tctggaatat
     3721 aaaattcgct gtttggatca agaatatgat tttgcatatc aataataact aacattttct
     3781 ttcctcctac attcatatcg tcattttatt ctttttaata gaagtttttt aaaatataag
     3841 caacaccagc ttcttcattc gtcagtgtta cttggtttgc aatttctttt acttgtcgct
     3901 gtgcattccc catcgcaata ctcaacccgc tgtaattcaa catagcaatg tcgttttctt
     3961 gatcgccaaa agccatgatg tcactcgatt cgattccaag gtaatccgcc agaaaaacaa
     4021 gtgaatgagc tttagtgata cctgtaggcg tgatttctaa gaaatgcgga tcagagaata
     4081 ctgattggat accatctgct tgcagatctc tttgtagctt ctgtagtttt tcttcactct
     4141 tatctgttaa tgctaattta agatagctaa gagaaggatc ttccaacata tttaaaattt
     4201 gatcaaaaga atggttctgt aaattgaaat gttcataata agggtcatct ttgactagat
     4261 ttgattcatc gaatgtcagc tgatcatctt gggtttctcg gctagcaatg atggatattt
     4321 tcgcttcttg agcatagaag aaggcttttc tcagtgtatc gctatctatt tttgaacgtc
     4381 tgagaacttt cgccattttt ccatccttca tttcagcaat cagtgaacca ttggaagaaa
     4441 tcaaatagcc accgattcct aattcggaaa aaatatcttt agcagaaatg ctgttccttc
     4501 cggtagcgac ggttacgatt ccgccatttt catcaaattt ccgaagtgct tggatatttt
     4561 cttctggtat ctttccattt cgatcaagca aggtaccgtc taaatcaatc gctgctagtt
     4621 tcattttatc ttcctacttt ctttttacat atctattttc ctgttacctt aagattaaat
     4681 gagaatttaa aaaaatgaaa ctaacttgct ttgtcattat gaattttttc aaaaaacatt
     4741 cttaagaaaa aggtggctat atatagtagt ttttggtcaa tacctttgaa taaaaagcca
     4801 tgaaaaacga atagttagcg cttaccgatg cgttttgtga taaaaaaatt ttaaaatatt
     4861 caatgaaaac aaagcatata ttttgcttta aaaccaaatg aggaatattc tagatgtaga
     4921 acaggttaga aaaagattag aaaaaggagt gattttgatg gagaagagat acggcgtagt
     4981 tcctgtggta actgcttcag acgaaaatta tgctccttac ttgagcgtga tgatcgcaac
     5041 tgcattagaa aattgcaata aaacaagacg cataaaattt tatgtgattg acgatggtct
     5101 atcagaatat agtaaagaag gattggaaga aacggtaaat aaatattcaa gtaatgcaag
     5161 tatccaattc ctgacagtgg aaaaagatat ctacgaagac ttcctagtca gtgaccatat
     5221 cacaactacc gcttacttaa gaatttcttt accaaactta ttagctaaag aggattataa
     5281 gaaagtcctt tacttagatt cagatgtgtt agtactagac gatatcgtaa aactatatga
     5341 tgagccgtta aatggaaaaa caatcggcgc tattatcgat ccaggccaag tgaaagcttt
     5401 ggaaagatta ggaatcgatt ccgatgactt gtacttcaat tcaggagtga tggtgattga
     5461 cattgatcaa tggaataaaa aagaaattac agaaaaaact atccattacc tcagtgaaaa
     5521 tggcgatcga atcatctatc acgaccaaga tgctttgaat gctgtacttt atgaagattg
     5581 ggaacaactt catccaaaat ggaacatgca gacttcgctg atttttgaac gtcatccagc
     5641 accaaatgaa aaatatgaac gtcagtataa agaaggaaat gaaaaacctt cgatcgttca
     5701 ctttacagga catgataaac cttggaatac gttgaaagat catccgtata ccaatttata
     5761 cttaaaaaaa ttagcacaca gtactctaac gaaagtaggc gagatcaatg aataaaaaag
     5821 aaatagcagt agtcgcaagt tgtaacacaa aattcgtacc acatttagct gcattatttg
     5881 tctcagtatt agataattgc aacccgtcta aatttgttcg gttctatgtg attgacgatg
     5941 atatcgattt tgaaagtaaa cagcttttac gcttctcagt gaaaaacgca cgaatgaact
     6001 cagatgtgga attcttgaaa atcaataaag aattctttac aaatgtggta atcagcgatc
     6061 gtattccaga aacagcttat tatcgtattg cgattccgga attgttccgc ggcacagaag
     6121 ttgaacgtat cttatatatg gattgcgaca tgatcgcttt acaagatatc tctaaattat
     6181 ggcgtcttga cttcggtgat tcaatcgtag cagctgttga agacgctggg ttccatcaac
     6241 gactggaaaa aatgaaaatt tcagctaaat caatgcgtta tttcaactca ggtttgatgc
     6301 tgatcaatgt gaaaaaatgg ttagatgaaa atattacgca aaaagtatta gatttcatcg
     6361 aacataatcc agaaaaatta cgattccatg accaagatgc attaaatgct atcttacatg
     6421 accgttggtt accacttcat ccgcgttgga atgcacaagg ctatatcatg gcaaaagcaa
     6481 aaaaacatcc aacagcagca ggtgaacgtg aatacgaaga aactcgcaac aatccttaca
     6541 ttatccattt ttcaggacat gtgaaaccgt ggagcaagga ttttgaaggg ccaacgaaga
     6601 aatattacga aaaatatgca gggatgacgg ctttccgttg tgtagctaaa ttccctaaat
     6661 atccaaaata tgcaaaagtt caaccaaaaa cagatgtagt tgttaaataa tagaagacaa
     6721 caactgaaga gggggagtag gaatggaaat tacaatgtct aaaaatgcag tagagacgtt
     6781 aattgaaaag gttggggaaa atacaaaaat cgcactcgcg cttatcaatg attccgatcc
     6841 atttctaagg gacaaaggtg cttttgcaaa aggcagcttt tttcaaatca ttccttttgt
     6901 ttcggaattt ggagaatatg cgactaaaat cgagcatcca ttattggaca tctatacatc
     6961 taaattagag caaaattatt tcggtaaacg attgaacatg gacttcaaca aacagcttga
     7021 cagcttttca ttagaaaatg aaatcgcagt actcgattat aatatcaaat taaagaactg
     7081 cttcttcagc taatagtttc gagaaagtcc aaaaaggagg tatacattga gatactactt
     7141 tgtggatgaa gaaaagaatg taatagaaac agatgaaaaa aaatataatt ggctcgtact
     7201 tgatagtgca aagcccgaag agattgagca agcaatcgaa gaattcgagc ttcctgatga
     7261 tattttcgta ggaaccgaat atccggaaga agtttctcgg atggagcgcc tttatgagac
     7321 gaaactagag catccatttt ctttggtagt gattaatcta gatcagtcag ataatcgtat
     7381 cgaaaaaaaa ttaacgccaa tatcttttgt actttccgat aatctcttga ttacttgtat
     7441 cgatagaaaa accgaatttg tggatcggct gctgacaaag catggaaatc atttaaactc
     7501 gtttgaaaag atcatcacgt atacattatt agatatttac acccattacg tgaaagaact
     7561 aagagagatg aaaaagcgaa ttgacgcttt ggataaagca gctcgtaaaa caacggaaaa
     7621 tgaagaactg tataaacagg cagatcttga aagagacatc gtatatatcg atcatacctt
     7681 aagagatcaa cgggaaacaa tggatcaatt atggaattct gaaactttca tcaaacgttt
     7741 agacgatgaa cagctactct atgatgtcca tttgagacag cgacaaacag aaaaaatgat
     7801 cgagatctat cgagatttat tagagagtat tggtgatcta ttcaacggga tgatggacaa
     7861 taatctaaat catatcatga aatatctaga ttcagctgca ttaatcattt ccgttccggc
     7921 gttgttcgca gggatttggg gaatgaatac aggaggatta ccgggtaaaa gttctgctct
     7981 aggctttata ctcgtagtct ttggctcttt gatagcagca gtagttttag gttaccattt
     8041 ataccggaaa gattatacaa attaaagaat ctatttaaaa caggtatcaa acaaagaaat
     8101 ttgttttggc aatacaattt aaaaactagg aggaatacag caatgacaga accaaaatta
     8161 atcgacccgc gaaacttgta ccatacggag aaatttccta aacaagatca ggatacacca
     8221 gctttacagc agaatatgat tcctgttcct gattgtggag aagaatccta tgaaggcaat
     8281 aatcaactag aaaaccgccg tgtactaatc actggtggag attcagggat cggtcgtgca
     8341 gcagcgattg catttgctag agaaggggca gatatcgctc ttcaattctt cccaggagaa
     8401 gaaaaagatg cagagg
//
LOCUS       DAZULG010000108         8392 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000108 DAZULG010000000
VERSION     DAZULG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.111"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..523)
                     /locus_tag="ACG9CY_002314"
     CDS             complement(26..523)
                     /locus_tag="ACG9CY_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS4938797.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(653..1363)
                     /gene="budA"
                     /locus_tag="ACG9CY_002315"
     CDS             complement(653..1363)
                     /gene="budA"
                     /locus_tag="ACG9CY_002315"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS4938798.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1376..3040)
                     /gene="alsS"
                     /locus_tag="ACG9CY_002316"
     CDS             complement(1376..3040)
                     /gene="alsS"
                     /locus_tag="ACG9CY_002316"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS4938799.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3245..3907)
                     /locus_tag="ACG9CY_002317"
     CDS             complement(3245..3907)
                     /locus_tag="ACG9CY_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4938800.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3917..4732)
                     /locus_tag="ACG9CY_002318"
     CDS             complement(3917..4732)
                     /locus_tag="ACG9CY_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4938801.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4994..5437)
                     /locus_tag="ACG9CY_002319"
     CDS             complement(4994..5437)
                     /locus_tag="ACG9CY_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFS4938802.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5571..5909)
                     /locus_tag="ACG9CY_002320"
     CDS             complement(5571..5909)
                     /locus_tag="ACG9CY_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4938803.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5897..6274)
                     /locus_tag="ACG9CY_002321"
     CDS             complement(5897..6274)
                     /locus_tag="ACG9CY_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4938804.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6498..7691
                     /locus_tag="ACG9CY_002322"
     CDS             6498..7691
                     /locus_tag="ACG9CY_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4938805.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7857..8279)
                     /locus_tag="ACG9CY_002323"
     CDS             complement(7857..8279)
                     /locus_tag="ACG9CY_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938806.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2462 a         1693 c         1413 g         2824 t
ORIGIN      
        1 ttcttttttg tttcaataga cgattttagt tattaataaa tgaaaaagca tttatattaa
       61 aatttgctaa taaaagatct acatattttt ctagaaatgc ttgatcgatt tcatcatacc
      121 ctttcgttac tccgcaatcg atatctaaaa caccgattaa attaccttct tttaccattg
      181 gcactacgat ttctgattgt gcggctgaat cgcaggaaat gtaatttgca tgggttttga
      241 catcatcaac gatcaatgtt tcttgttttt cagctgattc tccacaaact ccttttccca
      301 tcttgatacg tgtgcaagaa actcgccctt ggaacggtcc taaaatcaat tcattaccat
      361 cgaataaata atatcctgca aagacagtct caggcaaagt ctcattcaac aatgcagatg
      421 aatttgctaa atttgctaac cagttatctt ctgcttctaa taatccttgt tgctgtaata
      481 ataaaatttc gtatgcatct tttttctgtt tttctgtcca catgataaaa accgctcctt
      541 tttgatctat ggagaagatt atagctcagt ttttttgtaa acgctaggtt ttctcctcaa
      601 aaaaacgaga tacgtcaggt taatcacctt gaacgcatct cgctgtcttt ttctattctg
      661 cttgttggat ttctgctgct aaatcagcat aatcaatcgt ttcgtgaaca aaagtcggat
      721 catcaactgg aaaatgctgt acaagggacg agatgcggct aatctcaact tttccttcag
      781 ccacttcaaa atccattaca tgtccgccaa atgtatgtgt atcatcgata aaatgcaggt
      841 gaaatcccgc tgcagcaact ccttggaata attcaggcgt aaagaacccg actaatgttc
      901 cttggacatt gctttgttgg aattctggtt gagactctga aatttttgct agtcttctgt
      961 aaggtttttc ttgcttcggc atgatacgga tgtgcatatt tttgaataat ccagttatct
     1021 tcactgccaa aaataggttt tccccggctt ctcttactaa tatgtctttt ttcaattgtt
     1081 ctccatctgc agtttttgag acagaaaaac tagtatctgg cgtaaaatct gtaattgctg
     1141 catagggggt aagttcactt ccatctaatt gggcaactga gccatctgat cttccttgat
     1201 aagctacgcc atctaaaaag atgacttcac catcaagccc atgcaatgtt cccaatccca
     1261 gagttccttt ttctaatata tgagcaatcg tttctgttcc atccatcaag ccagccatca
     1321 aagctcctaa tgtcccgtgc tggtataaga ttttttcagt catgtcgaca cttccttaat
     1381 agaattgatc tggtaagatt gtttctccca atttctcatt atcacggtaa tcaatcggaa
     1441 tatcaataat tacgggtcct tctgtcgcaa atccttcttc taaagctttt tctaattcct
     1501 ctacacttgt agctcggatt cctttagcgc caaaagcttc tgcatatttc acaaaatcaa
     1561 ctggaccaaa gtctacacct gacgcccgct gatatttcat tttttcttgg aactctacca
     1621 tattgtagtg tccatcattc caaatcaaat gaacgatatt caatttttta cgtaccgccg
     1681 tttctaaatc ctgggcagag aataaaaatc ccccatcgcc ggaaactgat acgatttgtg
     1741 tatttggacg caccaatgca gcagaaatcg cccatggcaa tgcaacacca agcgtttgca
     1801 taccattact gaataacaaa tgacgaggtt cataacttct aaagtggcgt gccatccaaa
     1861 tataatgact acctacatct acggtaacag tcatatcatc tgttactttt gactgaagcg
     1921 tgttaataac ttctaaagga tgcaagatac ctgcttctcc ttttgattga acaatgtccc
     1981 gctccgttaa ttctgcttga agagatgcta aatattcttt tgcatcttct gacacttgtt
     2041 gaggctctaa tgaacctgtc aataaatcaa gagtggctga gatatcaccg atcaattctc
     2101 tttcaggctg cataaaagga tcgatttcag ctggcgcttc atcgatcaca atgatccgcg
     2161 catctttttc cgcattccag tttcttgctt catattcgat tggatcatag ccaatagcaa
     2221 taactagatc acttcgtttc aaaagcatat ctcctggttg gtttctaaat aaacccacac
     2281 gaccaaagaa atgcgcttct aattcacgtg agatcactcc agctgcttgg aatgtctcta
     2341 caacaggcaa tgctgtcttt ccaactaatt gcctgatggc taacgtttct ttttcacttg
     2401 atcctcgcat acctactaat agtaccggta gttttgcttc tctaatgcgt tcagctaggt
     2461 aacggatatc atctgcagaa gcagaaccta atttaggatc gctcaatggc ttgatgacat
     2521 tcccttggac aggtgcatcc actacatctt gcgggatact gataaagctc gcgccttttt
     2581 ttgaactctt agccatacga taagcattgg caatcacttc tgaaattgtt tctggatctt
     2641 ggatttctgc actgtatttt gtgattggct ggaacagtgc tgcattatcc atgctttgat
     2701 gggtcaactt caatagatcg cttcgtttta cttgtccggc aattgctaaa actgggtcac
     2761 cttctgctgt cgctgttacc aaacctgtag ctaagttact agcaccagga ccacttgttg
     2821 cgataactac tcctggctct cctgtaatac gtccgattgc ttgtgccata aatgcagcat
     2881 tttgttcgtg acgagtcacg attaattcag gaccttgatc ctctaattca ttaaatacac
     2941 catcgatttt tgcacctggt ataccgaata cataatcaac atgatgatta atcaaacttt
     3001 ctactacaga tttcgaacct tgtttcattt tttcgtccat tgaacatcgt cctttttatg
     3061 ctttattttt aattattagt tagaaataat cacaaataat tgatacagtt tctaaacctg
     3121 tttcattcaa aagtatagca cgttttattt catttgttta gccatatgtt tcaaaaaatg
     3181 aaaaagaatg acagttttta cataaaaact gtcattctta attgaaagtt tcaatttata
     3241 aagtctacag atccatagtt ctcatcaatt tacgattgtt gaagtaaatc aaaccaaata
     3301 cgatagaagg acctagtaga atacaccatt ggggctttac gaaaaagaag agaaacaaac
     3361 ttgtcaatag taaaaaatct agtaaactca cataccattt tttcaacgta tagtagaacg
     3421 ctatccgtag aacttgattt ggtttcgacg ctggatttct cacctgaaaa taccagtagt
     3481 tgatgctaaa ggaaactgat aaacaagcaa gtaaaacaaa caacggaatc aaccattttc
     3541 cataagaaaa acgtataaaa aagaaacaat caaaaatcat tatcacacta actatccaag
     3601 caaatagcca ataaaaaaat ccctttagcc aaaattggcg aaacgtcttt atgaacaaac
     3661 gtactggtga aaaacttttt tcttttgcta attcttctgt catacctatc atcacgatgc
     3721 aacctggacc aataaaaagt aaagaaaaaa taaaggaaaa gacattcctt ccatctatcg
     3781 acaaaaggaa cgcagctgct atatacaaga tattgacaca ccaaaaacaa aggctacttg
     3841 ccaatgattg atacaaccaa taaaagaatt ccatataaac atttttgtca aaacgctgct
     3901 ctttcataat cacctcctat tccttcattc ctgatgaagc gatcccttct acaaaatatt
     3961 tttgcatcgc taaaaatata aaagcaacag gaagaacaga aacaactgag gcagccatga
     4021 ttaatgcgta ctctgcatca aatagcccaa taaacatttt caaccctaac tggattgttt
     4081 tattttccgt agaagacaaa taaatgaatg gccccatata atcgttccat gtattaacga
     4141 atgtgatgat cgtcaaactg gcaatcgccg gttttgtaag aggtaaaatg atcttgcgat
     4201 agattcccca ctctgataag ccatcgatcc gcgcactctc acataaagaa tctgggatac
     4261 tactgtagta ttgcttcaac aagaacacac caaaagcatt gaacgcttgt aatagcacca
     4321 acgaccacaa cgtatatgtt aaacccactt ttctcatcat aatgaactgc gggatcatat
     4381 aagactgcca agggacagca atcgtgccga tatatgctaa aaataaaata tcgcgccctt
     4441 taaaatttag tttagaaaac ccgtaagcag caaaactaga agtaaacaac tgaatcaacg
     4501 tgatcacaac gcttaaaaaa gctgtattct taaaaaatgt cactaaagga atacgagacc
     4561 aaataaccag ataattctct aaatgccaag ttttagggat ccattgaatc ggaatcgtga
     4621 acacttcatt atttgctttg aatgaagcag aaaccatcca taaaaaagga atgatcgtca
     4681 ataaagctaa tacaatcagc agaatcatac ccaaccattg aataaagggc atgttttttg
     4741 aacgaatttt tgcttttttt ctttcatttc gaacacctcc aaagttataa tcttcatttc
     4801 tttgtaatga ttacaatcca gaacaacggt tattatagct gattcatttc cgttcttctt
     4861 atgtttgtaa gcgcttattt aacaagttat gtaaatctct ttattttttt tgatttaaaa
     4921 atgaaaaaat acaaaagcaa gactgtcata cgtctcgctt ttgtattgaa atttcttcga
     4981 ttagagatac ttattactgt tcaaaagctt ctttgaaacg gtgaatcaat tcattgacta
     5041 caggtgataa aatccgattc tttttccaga ctagcatgca gttcgtttca atcattgggt
     5101 caagcggaac gaatgtcact cgctttgaaa gatgacttac agcaccttct atgactaaag
     5161 cagcacctac ctgattctca attagcgcaa atacattgaa aatcaaattg tacgttcctg
     5221 tgatcagcaa ctgctccttt tttatgccta accagtcagc aaacattcgc tgtatttctg
     5281 gacgctttga gatcaaaaga ggtacattcg caaagtcttc ttttgttatc acgtcttttt
     5341 ttgctaaaac agaattttct aaaatgagca atccccattt ttcctttctt ggtaacaaaa
     5401 tcgtttcgta tttctctgta tctactggat ctagcaaaat cgctaaatct aacaggcttt
     5461 tttctagtca ctccgtgata tcatcacttg ttccgctgtg gatatgaaat tgaacgctgg
     5521 gataatcacg gatcaacttt tctagtaata gtgccaatgt attcgaatta tcagcttcca
     5581 cacagccgat cgtaaatcgg ccgctgaata attgtttttt tctgttctcg aattcttgag
     5641 tggtttgatt cgttaaatct aaaatttctt ctgctcggct tttttagaaa ataacctgct
     5701 tctgtcaaga tcattttgcg gttttcgcgt acaaacagtt ctgttcctag ttcctcttct
     5761 aactccttca gttgtcggct taaggttggt tgtgtaagat gtagaacttc tgcggcttga
     5821 gagattgttc cttcttctgc aattgtccaa aaatatttta gtaagcgtaa ctccatagcg
     5881 atgctccttg atttttttac ttgatacatt ccattttatt tctagataac acacgatcat
     5941 atttcttgat ttttccttgt aaacgttcca aacattcttg catttcagca atttttttcg
     6001 tcaaaagttc actttcttca atcaaaagct tacgccgctc aaatacagtt gcttctcctt
     6061 cgctagatag gcgaatatat tcaatcaaag actcgatcga taatccggca tttcgcatac
     6121 agatcgtaaa gtcgacccat tttaggtctt cttctgtata atcacggatt ccgccttttg
     6181 tccgattaac tggtggaatc aacccgattc gttcataata gcgcaatgta tcggccgaaa
     6241 tgcccttttc ttcactgact tgtttgatat tcatctgatc actctttctc ttaatttctt
     6301 gccactttac ttatgtattt tatttattct acaactttaa gtgtactcca agtcaatact
     6361 catttctatt aaaactaagc tggattatct atatcggttg tttgtgtatt atttcaaata
     6421 aaaaagtctt gtttttttat tcttttttgg atatactttt aacaatttag ggaaatttta
     6481 ggaaaagggg tataaaaatg acaacgtatc agcgaaagtt attattatcg acatcggcgg
     6541 gaattgcttt agagaatatg gatattatgt ttttagcttt ttcattatct tcaatgatta
     6601 cttcttttca tattagtggt acgcaagcgg gtttcatttc aacgatcact aatttgggga
     6661 tgctcattgg aggtattttt tttggcatca tagctgataa atatggacgt gtgaaagtat
     6721 tttctcaaac ggtccttctg ttttcgattg cttctttatt gatgtatttt gcttcaaaca
     6781 tctacttggt ttatctcttt cgctttatcg caggtgtagg agcgggagga gaatacgggg
     6841 cgtgtatgtc cttgatttcc gaaagttttt caaaaaaaca aattggacga gcttcttcgg
     6901 ttgctggtat tggtgcgcag gtcggtgcgg ctttggctgc gattttagct gctgtgatca
     6961 ttccaacgtt tggttggaag atgttgtatg tcgtaggtgt actgccggtg ttgatggtct
     7021 tgttcattcg tcgcggcttg actgaaccaa aggaattcaa atctgtacaa tctgccgaaa
     7081 agaaaaccaa acttagcaga ctgtttgaat caaaacatct tgcttggcaa acatgtggcc
     7141 ttagtatcat ggtaactgtc caaattgccg gctattttgg gttaatgaat tggctgcctt
     7201 caattatgca aaatcgatta gggctttcgg tttctgggtc ttcattgtgg atggtcagta
     7261 ctattttagg catgtctttg gggatgatga cttttggcat aatcatggat aaattggggc
     7321 ctaaactagc cttttctttg tttttaattt gttcatcctt atctgtctat ctacttgtgt
     7381 tagcccatag ccagtggagt ttggtagcag ccgctgtgat agtcggctac tttatcaatg
     7441 gcatgtatgg tggatatggc gcagtgatta gtagtttgta tccaacagaa atccgcgcga
     7501 cggcgaataa tttcattatg aaccttggac gagctgttgg tggtttttct tctatcgtca
     7561 tcggtttctt gatggatcat tatagtttaa ctgccgtaat cttgtttttg agcggtattt
     7621 acctagtcag tctgctggtc ttgacaactc ttactggtgt tacagcatta agggaaagct
     7681 tgcagcattg atagttgagt ctgtaaatga caagaaagag gtgtggcaaa agcttgcatc
     7741 tgcggtaata agaccagcca aatcaaaaac atgagacact gtttccgttt ggctggtcgt
     7801 attacttgat gcagggcagc tttttcaact gctaatttcc ttctatctgg cggctactat
     7861 tctttgacgt ccgtcatttc aaagtacttt actaataact tagcataacg aaaaactgtg
     7921 gatacactcg tatattgagc gtctgcataa tcttccaaat ttttctcagg atgttgatac
     7981 atgaattcaa tcaaatgata attatatgat tttctgtaga aatgaagata aataatgtag
     8041 attgggaagt tctcctgctt ttccatagaa aagcgccgat tattctcatt cttcaaaatg
     8101 accttatcag gatatttttg gaacaattct tctaaatctg ttagataatt caagatagac
     8161 ttacttgata ccttcaattc gttttgtagt tcttctattg aacaccaacg ttctgtttca
     8221 agcagtaaat gaatcatttt aattttcttt tgagaaactt ttgttaaaaa agtctgcata
     8281 ttttctcctc ctgtatctac tttactatac tacatacaag aaaaaaaacg gatacaaata
     8341 tcatattgtc acaccgtcaa tagtgcataa acctgttatt atttctcttt tt
//
LOCUS       DAZULG010000109         8306 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000109 DAZULG010000000
VERSION     DAZULG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.112"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..343
                     /locus_tag="ACG9CY_002324"
     CDS             119..343
                     /locus_tag="ACG9CY_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS4938807.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            485..2236
                     /locus_tag="ACG9CY_002325"
     CDS             485..2236
                     /locus_tag="ACG9CY_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS4938808.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            2236..4020
                     /locus_tag="ACG9CY_002326"
     CDS             2236..4020
                     /locus_tag="ACG9CY_002326"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4938809.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            4478..5329
                     /gene="licT"
                     /locus_tag="ACG9CY_002327"
     CDS             4478..5329
                     /gene="licT"
                     /locus_tag="ACG9CY_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4938810.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            5426..7312
                     /locus_tag="ACG9CY_002328"
     CDS             5426..7312
                     /locus_tag="ACG9CY_002328"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4938811.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(7353..8255)
                     /locus_tag="ACG9CY_002329"
     CDS             complement(7353..8255)
                     /locus_tag="ACG9CY_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4938812.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
BASE COUNT         2602 a         1442 c         1797 g         2465 t
ORIGIN      
        1 ttctctaaaa aaaccactat tttttgcatg aaactaaaga atacgccttt cttttttcaa
       61 taaactttag taaaataaat cattgagagc aattcggaaa tagaaaggag agatttatat
      121 ggtatcaaca ggtattgtcg tacttattgc tattattgct ttgctaatcg gcgcagtagg
      181 tggctttttc ctagcgcgta aatatatgaa agactattta gaaaaaaatc ctcccgttaa
      241 tgaggacatg ttgcgatcaa tgatgatgtc aatggggcaa aaaccatctg agaagaaaat
      301 tcgccagatg atgcagcaaa tgaagaacca agggaaaaaa taggttctga cccaaagact
      361 ttttgcgggc tgattttttc agtccgtttt ttatttcgta atttgttaga ctattcagac
      421 tattcagcaa aaaagacaag cacagacaat cctatgctct tgataaagac aaaggaggag
      481 taaaatgtcg atttttagaa aattaggttg gttttttcga caggaaaaga aacattattt
      541 aattgggatt ttctccctat tcgcagtggc cttggtacag ttagctcctc caaaagtgat
      601 tgggatcatc attgatgaga tcgccgataa aaatttatca ataaagatca tttcctggtg
      661 gatcttatta ttgatccttg cagctctctt acaatatctt tttcgttata tctggcggac
      721 gaatatttgg ggaagtgcag ctcggttgga gaaagaactt cggcagcagc tattcaaaca
      781 ttttacagaa atggacaatc tcttttacca aaaatatcgg actggagatt tgatggctca
      841 tgcaacgaat gatttgaatg caatccaaaa tgtagcagga gctggtatct tgacctttgc
      901 agattcagtg atcacgggcg gaacaacgat cattgcgatg attttgttta ttgattggcg
      961 cttaacactg attgcgctga ttcctctgcc tttactagcc gttacttcac gagtgctggg
     1021 gtcaaaactc cacgatgctt ttcgtgattc gcaagaagct ttttctagta tcaatgataa
     1081 gacacaagaa agtattactg ggatcaaagt cattaaaaca tttggacaag aaaaagaaga
     1141 tctcgcggat tttgaagaaa agattgaaga tgcaattatt aaaaacaaac gagtcaattt
     1201 tttagactcc ttatttgatc cattcattac cttgattatc ggtctgtctt atgttgcaac
     1261 aattatttta ggtggaaagt atgtcttgga aggcacgatc tcaatcgggc agatggtctc
     1321 atttattagc tatatcggta tgcttgtttg gccgatgttt gcgattggtc gattgttcaa
     1381 cgtattggaa agaggaaatg cttcttatga tcgcgtcaat gaacttttgc atgaaaagac
     1441 gcatatcgtg gaacaactgg gtgcagtgca gactccggca aaaggcgagc tgaatgtagc
     1501 tatttcctct tttaactatc cgaaagaaga atcaccagca ttagaacaaa tcaaatttgc
     1561 gatccaagaa ggagaaacat taggtattgt tgggaaaact ggtgcgggaa aaacgacaat
     1621 catcaaatta ctgatgcgtg aatacgatga ttatcaagga aaaattcaat ttggcggatg
     1681 ttcgatcaaa gaatattcat tgaatgcttt attaggatca atcggctatg tgcctcaaga
     1741 tcacttcttg ttttcgatga ccattcgaga taatatccgt ttttctaatc caaaactttc
     1801 agaagaagaa gtagagcatg cggcttcttt agcatttatc aacaatgaaa tcaaggaatt
     1861 accaaaaggt tatgatacct tagttggaga acgtggagtc tctttatctg gcggacagaa
     1921 acaaaggatt tcgattgctc gagcattgat caaaaatcct gaattgctga tcttagatga
     1981 tgcactttct gcagtagatg ccaaaacaga agaagctatc ttgcataatt taaaagaaat
     2041 aagaaaagag aaaacaacca tcattgcagc tcaccgttta agcagcgtga tgcatgcaaa
     2101 agaaatcatt gttgtcgatc aagggaaaat cattgaacga ggaacccatc atgagctgct
     2161 tgcattaggc ggctggtatg aacgaatgtg ggagaaacag aaattagaag cgaagataga
     2221 aggaggcgag gcctgatgga tgaaaattat caatcggaat ggacaaaatc gattccactt
     2281 aaagaacaga agaagatcat caaacgactg atgaagttcg ccaaaccttt tagaagaacg
     2341 ttcatcgttg ctatcttatt tgcgtttgcc ctgtctgtga tcaatgtcct attgccaagg
     2401 atcatccaaa cttttatgga tgatcatctg gctaaacagt cggctacgac acaagttatt
     2461 ttgttttttg ctggtatgta cttgtttgga gtcatagtca aaagtatcat ttggtttttc
     2521 caatggtatt tgtattccat ggcttcattg aagacctatc agtatgttcg ggtaagatta
     2581 tttgaaaagc tgcatacact aggtatgcgt tattttgatc agacaccggc aggatcgact
     2641 gtttctcgag tgacaaatga cacagaaacg ctatttgaat tttggtatgt ctttttgatg
     2701 gtattgactg ggatctttgc agtcgtttct tccttcattg ccatgtttca aatcaatgga
     2761 gaaattgcct tgtggacttt ggtgttcttg ccgattttag gaatcgtcat ctggtattac
     2821 cagaaattca gttctcgtat ttaccggaat atgcgggaaa aattaagtca gctgaacacg
     2881 aaattgaatg aatcgatttc cggcatgcgt attatccagc agtttcgcca agaagctcgt
     2941 ttagctaaag aatttgaaca agtgaacaac gagtatttag acacgcgcta tgcgatgatc
     3001 aaaacaaatt cgattctgtt aagtccgatc atcaattttc tatatgcatt ggcgattgcg
     3061 ttggcgttga ctttgtttgg gatcgatgcg ctgcattctc ccgtagaagt tggactgatc
     3121 tatgcattta cgacctatgt ccagggattt tttaatccaa tgtctcaaat gatggacttt
     3181 ttgagcacct ttacagatgg aatggttgcc ggtagtcgta ttttgaaaat catggataca
     3241 gaagaatatg caccgcaaca aaatcctgga gcagatcaag cgattacttc aggtaagatc
     3301 gagtttcgca atgtaagctt ttcttatgat ggagagcaca atgtattgaa caatatctct
     3361 tttgtcgcta atccgggaga gaccgttgca ttagtggggc acaccggaag cggaaaaagc
     3421 tcgatcatca acgtattgat gcggttttac gaattttatg aaggccagat cttgattgat
     3481 gatcatgaca ttcgagaata tccaatggaa gagttacgta agaaaatggg attggtcttg
     3541 caagatgcct tcttgtttta tggagatatc gcaggaaata tccggatgat gaatccaact
     3601 atcacagacg aacagattcg ccatgcagct gagtttgttc aagcaaacaa gttcatcgat
     3661 gaattaccaa aaggatatca ctcaaaagtg atcgaacacg gtgccagcta ttctagcgga
     3721 caacgacagc tgatcacttt tgcgcgaaca atcgtgacag atcctaaaat actagtattg
     3781 gatgaagcga cagcaaatat cgatactgaa acagaaggat tgatccaaga aggattgaca
     3841 aaaatgcgac aaggacggac aacgattgcg attgcccatc ggctgtccac gattcgggat
     3901 gcagaattga ttttagtttt agacaaaggt catatagtag aacgtgggaa tcatgaaaca
     3961 cttctagcgc aaaatggttt gtatgcagat atgtacaaac tgcaaagcag tggtgtgtaa
     4021 aaagtggttg tgacagggct ccgttcagct ttaggtgata agaaaaaaat tgtaaaaaag
     4081 gctttccaga tttttgcata aatttggctt atcaccgata agctggtact ggaacaccgt
     4141 atattagaag ttgggacagt agcatagtag cattttgacc taagcgctag agtgaaaata
     4201 aaacagagct tcccagattc ttcatctgtg agcgttagtg tcgtaattac cagatttaaa
     4261 aaaaatcacg acagctagtc agaagtgccc aatagccgct aagaaagcgt tgacaaaagt
     4321 gcttgctttc gataatataa agaaaacgat acaacttgga tagtgattgc ctgatgcaag
     4381 ctaaacctga tgagtctccc atggttccat ggacggcttt ttgggttttt ctttttgttt
     4441 tggatacagc cctatatatc atggaaagga gtaaaacatg ctgattgaga aagtactgaa
     4501 taacaacgtc gtaataacca gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg
     4561 tttggcattc aaaaagaaag tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt
     4621 tcagttgaca aatgacggac tttcacaaca gttccaagaa ttggcacaag cgattcctct
     4681 tgagtactta gaaatttctt gtgaaatcat tgaatataca aaagaggctc tacaagtcca
     4741 gttgaatgat tcggtgtata ttgcattgac ggatcatttg tatacggcta tcgaacgtca
     4801 caaaatggga atatttgtac caaatgtatt gctgtatgat atcaaacaac tatttcctaa
     4861 agagtatgcc atcgggaaaa aaacagtgaa gaaaatctcg gataaatacc acatcaagtt
     4921 atcagaagat gaagccggat ttatcgcatt gcatttagtc aatgcacaag gcattcaaag
     4981 ccaacaagac aatacctacc tgtttactac aacaatacag gatattctaa atatcgtccg
     5041 ctattactat aagattacat ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca
     5101 cctacgtttt tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc
     5161 tgaggaattg taccaggtta ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa
     5221 aatagcagac tttttagccg agatattcaa ctaccagatg tccacggacg aaaaaatgta
     5281 tttaacgatc catattgcgc gtttagtgca tgagaatcgt tgtaaataaa taaaaaaagg
     5341 aggataaatt aacaagaacg catgaaataa gggtagtgac attcagttgg ctaaaccttc
     5401 cactaataaa ttggagggaa tagaaatggg aaaatatcaa gaattagcga gcaaaattgt
     5461 agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca cattgtatca caaggcttcg
     5521 tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata tggacggtgt
     5581 tgtgacggta atgcgagcgg gaggacagta ccaagttgtc atcgggaatc atgtgccagt
     5641 cgtgtttgaa gaggttataa aagcaggaaa ccttactttt gatgaggctg tttcaacaga
     5701 aaaaatgaga ccgtttgata tgcttattga tggaatatcg ggctgtttcc agccattcct
     5761 tgctattcta gctgctggtg ggatgattcg tggactgaca gcattacttg tcttcttagg
     5821 cgcatttgac cgcggttctg gcacatttgt tatgtttgat aatatcggtg attctgtatt
     5881 ccaatttatg cctgttatta tcggtttaac agctgcccgt aaatttaaag taaatgaatt
     5941 cgtagggatg ttgcttgggg cagcattaat gaatcctagc ttgtcgctgg aagctttgag
     6001 tggtgcagca gaagcaccac tgactaccat attttctggc actatttttg aagcacctat
     6061 ttatcaaaca gtattcggca ttccctggat tgcgcgtaac tacgcttctt ctgtcattcc
     6121 cattatcttt attgtgttgt tagctagcca aattcagaaa ccaattaaaa agttagtacc
     6181 tgagatgatc gcgaatttct tcgttccatt cttcaccgtt cttattacaa tgcccttagg
     6241 ttttttactc gttggaccag cattcacatt cgcaactgat attttaatgg ctggatttga
     6301 aacactttta gcactttcgc cagttattta tggagcaatc gtaggcttct tctggcaaat
     6361 tctagtgatg tttggtctcc attgggcaat cgtaccaatg ggattgatgc aattttctgt
     6421 gaatggttgg caaaatatca tgacacctgt tgcagtagtg tcttttggac aaactgcagc
     6481 acttactgca ctctacttta agcttagaaa cccaaaagac aaagcaatcg caataccagc
     6541 cattgtttct ggtattgtgg ggattacaga gccagctatt tatgggttta cattgccacg
     6601 taaaaaaata tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt atagtggatt
     6661 aatgaattta acttcttgga atccaggtgg attaggcatc tttacaattc ccaactatat
     6721 tagaccagat ggtgatttaa cggatgtgat taatgtattg attggaatcg ccattgcgat
     6781 ggtggtatcc tttgcgctga cttttttctt ttggaaggag gaagcaggtg aaacagatga
     6841 tagacagaaa agatcaggta aagaaattgt aaaaacaccg atacaaggac aaatcgctcc
     6901 actgaatgca gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag gaatcttgat
     6961 ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga acagtcatga ccctgttccc
     7021 gacgaaacat gcaatcggaa tggtttctga agcaggtctg gaattgctga ttcatgtcgg
     7081 attggatact gttcagttgg aagggaaata ttttgaaagc ttggtgcagc aaggagaaag
     7141 agtgaaacaa ggcgatctgt tgctccgctt cgacaaaaag aaaatcgaag aggaaggata
     7201 cagtttagaa acacctgtga tcgtcaccaa ttacacggat tatttggata ttttggaaaa
     7261 tcaaggggag acggtcgggc cagacgatgc acttatcacc gcactgacct gaacggttag
     7321 gtgaaagaat ggactaggct taattattct ttttagtcca cttctttatt ttcaaaattg
     7381 aaaggctctt tcatgatggc tgagctgata gggcgtttat caaatttttg tcctacagaa
     7441 attagatgga ctaaattctg ttgataattt tctgcaaaaa cacaactata taacacgtct
     7501 agaagataac aaatcgatgt attgatcgta aaattaccaa ttttcgaata cagtcgttcg
     7561 cgtgtcgtaa gtcgcaaagc ggctgtactc attgaagata gcgttgaatc accgatactg
     7621 gtcaaagcta aaattggaac atgattttgt ttcaatattt ctgcggtacg aaggatcaca
     7681 ttgttttcac ccgtataaga aatcagaatc ccgcacatat cactcgtgca attgtacgct
     7741 tcatagaacg attcaccaaa agtggtgaca atcgtgacat ccattttgat gcgcttcatt
     7801 ttcagcatga aatcttgaga aatcaaagta ttcgagttac tggcgaaaat cacgattttt
     7861 tttgcttgaa gcattagttg tttggctttt tgcagttggt catgatgaat gagagaaagt
     7921 gtatcttgaa tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt cataataccg
     7981 tctgtggtgg aaaaaggaag attcgcatct acgtcttgaa aattcccttc aagataggct
     8041 gtttcagcta aaaaagcatt acgaaaactg acccagccat caaaacccat tttttttgct
     8101 acacggatga gtgtggacgg gtggacataa catcgctcag caatctcttg gatggtcatt
     8161 tcgccaagtg ctggagaatc gcttaagaga aactgaatca cttcattttc tgaaggagaa
     8221 aaggagattt ctttgatttt ttcacgtaat aacatgtaga taccttcttc ttgttttttt
     8281 acgtaaatta taactttttt gaaaat
//
LOCUS       DAZULG010000110         8256 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000110 DAZULG010000000
VERSION     DAZULG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.113"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(212..1660)
                     /gene="cls"
                     /locus_tag="ACG9CY_002330"
     CDS             complement(212..1660)
                     /gene="cls"
                     /locus_tag="ACG9CY_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4938813.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1812..2696)
                     /gene="ftsX"
                     /locus_tag="ACG9CY_002331"
     CDS             complement(1812..2696)
                     /gene="ftsX"
                     /locus_tag="ACG9CY_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS4938814.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2689..3375)
                     /gene="ftsE"
                     /locus_tag="ACG9CY_002332"
     CDS             complement(2689..3375)
                     /gene="ftsE"
                     /locus_tag="ACG9CY_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS4938815.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3480..4581)
                     /gene="prfB"
                     /locus_tag="ACG9CY_002333"
     CDS             complement(join(3480..4508,4510..4581))
                     /gene="prfB"
                     /locus_tag="ACG9CY_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS4938816.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4707..7241)
                     /gene="secA"
                     /locus_tag="ACG9CY_002334"
     CDS             complement(4707..7241)
                     /gene="secA"
                     /locus_tag="ACG9CY_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS4938817.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7463..8014)
                     /gene="raiA"
                     /locus_tag="ACG9CY_002335"
     CDS             complement(7463..8014)
                     /gene="raiA"
                     /locus_tag="ACG9CY_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS4938818.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8142..>8256)
                     /locus_tag="ACG9CY_002336"
     CDS             complement(8142..>8256)
                     /locus_tag="ACG9CY_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFS4938819.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
BASE COUNT         2202 a         1819 c         1440 g         2795 t
ORIGIN      
        1 actctcatgc tatactggct ttgaaatgtg tctcttttaa tagcatatat tagcttattt
       61 cttgctttcc ccaaaaaaca agagataccc caaagttaat ttcgctcatg cgagacgctt
      121 tctccatttc tgacgtactc tcagaaatgg ccccgttaaa aaaggacacg agatctccat
      181 ctgttgatgg ttctcatgtc ctttttatgt tttaaaggag cggcgaaatc aatcttgcta
      241 atccttcttt catcttgatg attattcctc ggttttcata catttctttt gtgagatgcg
      301 tagaaatttt cgagtcttca aaaaaagctt ttcttacacg tgaagccatt cgttcatcat
      361 aaaccagtgt attgacctca aagtccaagc gaaatgaacg aacatcgata tttgctgagc
      421 caactgaaac aatgccacca tcgataatca tcgtctttgc atgaataaat ccattctcat
      481 atgtttcgat ccttgctcca tactcgatca gctctgcagc gaaagaatac gttgcccagt
      541 aaacgagaag atgatctggt ttgttaggta tttggatatg aacttttacg cctgaaagaa
      601 gagccaactt cagtgcttca tgaatcgatc catccggtat gtaataaggc gtttggatca
      661 atatctcccg tttggctaac gaaatcattt tcaaataagt cattttgatt tgttcatgtt
      721 cggtatctgg tccgcttgtc acgatttgaa cagctatatc accagtagtg tgaatggacg
      781 gaaaaaattt gggagaatag cctacttctt ttgtatgctg agaattccag tccatcaaaa
      841 aacggttttg caaggtatac accgcttctc cgtaaatacg caaatgattg tctcgccaat
      901 aaccaaattt ctttaccaat cctagatatt catttccaac attgaaccca cctgtatatc
      961 cgatttcccc atcaataact acgattttac gatgattgcg ataattgatc cgaggattaa
     1021 tatacggaac aaatagagga aagaaaaatg cgacttctcc gcctgctttt ttcaaattct
     1081 gaaaaaagct ggaagaaact tgtgtggaac cccatgcatc aagaaggact ctgactttta
     1141 cccctcgtct tgccgcctct gtcaatgcat ctcgaacttc ttcacctaat gcatcactgc
     1201 gataaatata atactggaag tggatatggt cttctgcctg cttgatatct tcaatcagcg
     1261 catcgaattt ttcacgacca tctgtaaata aagtgatttc atttttagtc gtatagagcg
     1321 tactttcaaa taccgtgagc atatagatca attgtttcac atctacttgt ccagtaggcg
     1381 gatgaggata aagccctcgc atcaatgcct gtttgtcttc ttcaatttcc aggttcttac
     1441 cgattttccc ttggatcttc aaatcaaaga ttttctcttt ggaaatccct cttcctaaaa
     1501 aaatatataa aataaatcct acaactggaa tgaacatcaa aacgagcagc catgcccatg
     1561 tttgagcagt ttcctttctt tctcgaaaaa cgatgataaa ggaaagcaaa atattcataa
     1621 tcaaaaggat cgtaaaaaaa ttgtcaacga taattttcac ggcttcacct cttcttctac
     1681 caacttaaga atacccaagt tgttttttaa agtaaaggga aaaaagccgc ttccttctat
     1741 tctcctagcc aataaaaaaa gaaagctgga acgaaactgt ccaactttct cattttattt
     1801 atattgtggt attagaattt caagaaacgt ctcatagaaa tgatcgagcc aagtgaacca
     1861 atgatcacac caatgccgac cattaaaaga ttgattttcc aaacgatatt ttgtggatga
     1921 atcaatgaat aatgggaacg taataatgat ggattgatca attggaaaaa ccatgcataa
     1981 ccaaatgtga tgatcaaaat tggaagaatt gcaccgatca gaccgatcca gccaccttca
     2041 aggaaaaacg gccagcgaat atatccattt tttgcaccga ctaaacgcat gatttgaatt
     2101 tcacgctgtc tagacaaaat cgtgatccga atcgtgttcg aaatgagaaa catcgctacg
     2161 aacagcagca atgccgctgc aactaatccc catgtccgaa ctgcaccagc gatttggaaa
     2221 atccgatctg aggaagctcc accgtaatca gaacgagaaa cattcttcaa atctgctgct
     2281 tcttctgcta cttgttttgt ataagaagga tcagtagcac ttactacata cacatcatat
     2341 aacgggttat tgtcaccttc aaacaatttc caggaatcac ccatttcgtt ttggattttt
     2401 ttcaattcct gttctttgct tgaaaattca acttttttaa catgatctaa gtcattcaat
     2461 tctttttcta gctttttcat ctcttcgtct tttgtaccga tatctacgaa tacagataca
     2521 tcaacgtttc cttctatatc ctgagctaat ttagttgcat tcatgataac tgccatgaat
     2581 acaccgacta aagtcaacgt gatcgttacc gcactgactg aagcaactgt catccagccg
     2641 ttacgtttca aactcttgat actttctaat aaatggcgga aaaaggttct aatcatcgta
     2701 tccatattcc ccttccgctt gatctcgaat aatgcggccg ttttcgatag caattactcg
     2761 gtgccgaatc gtatttacga tcgtactgtt atgtgtagcc atcacgacag tagttccttg
     2821 ggcattgatc cgatctaata atttcatgat ttcccatgag ttttctggat ccagatttcc
     2881 tgtcggttca tccgcgataa gtactttagg cgtattcacg attgcccgag caatagagac
     2941 acgttgctgt tccccaccgg ataattcgct aggaaagact cgaactttgt gtttcaaacc
     3001 aacaaggtcc agcacttcca ttactcgttt tttgatatct cgaggttttc tgccgataac
     3061 ttgcatagcg tacgcaacat tttcatatac tgtttttctt ggtaataatt tatagtcttg
     3121 gaacaccacg ccgatttctc tgcgtagata aggaacttcg cggtttttga ttgctattag
     3181 atcatgacct gcgacgttca aagttccttt tgaagctttt tcttctcgat acatcaattt
     3241 gatgaatgta gatttaccgg ctccagacgg accaaccaca tagacaaact ctccttgttc
     3301 gatgacaatc gagagattgc ggatagcggt cgtaccattt gagtacttct tcattacatc
     3361 cttcatttca atcatggcgt tctctccata tctttttcag tctttagcta ttatagcatg
     3421 gcaatgtttc aacatgcttg caaaatctta caattttatt tcaatttaac aatgggttct
     3481 tattgtagat gcatttttaa atagccgtca ataaataaat caaggtcacc gtccataact
     3541 gcttggacat ttcctgtctc tacgtttgtc cgatggtctt ttaccataga atagggatgg
     3601 aaaacataag aacggatctg tgaaccccag ccgatttctt tttgttctcc tcgaagtgca
     3661 gctgcttctt gttctttctt ttccacttct aattgataaa gcttagcttt taacatcccc
     3721 atcgcttgct cacggttttt caattgtgag cgttgtgcct gactagctac tactgttcct
     3781 gtaggcaagt gagtgatccg tacagcagat tctgttttat tgatgtgctg accacctgca
     3841 ccacttgctc ggtacgtatc gattttcaaa tcatctggat tgatttctat atcgatcgtt
     3901 tcatcaagct ctggcatcac atctaccgaa caaaaagaag tatgacgtcg tttcgctgag
     3961 tcaaaaggcg aaatccgtac aaggcgatga acaccttttt ctgattttag ataaccaaaa
     4021 gcgttatgtc ctttaatcag cagggtcaca ctcttgatcc ctgcttcatc acctgcttga
     4081 taatctagtg tttctacttg atagccatgc tgctctgccc aacgtgtata catacgtaaa
     4141 agcatgctgc cccagtcttg ggactccgtt ccgccagccc ctggatgcaa ttcgacgatc
     4201 gcattgtttc gatcataagg tccgtctaac agcatagata attcatacgt cttcattttt
     4261 tcatctaatg cattgatacg tgtttctgct tcttcttgca ttgctgcatc aggttcttct
     4321 tgcagcatct ccaccatcaa ttcgatttct tctagttctt cggcaagaga atggaattgg
     4381 tcataggttt ctttattagc gttattttcg ctgatcactt tttgtgcggc ttcgctgtca
     4441 tcccaaaaac caggttcagc catttttgct tctgcttccg cgatgtcttc ttccagctga
     4501 tctaagtcaa agagacctcc tgaaacttgt gaccttttcc tgcatttctg tcaggagatt
     4561 tcgaatttcg gatatttcca tgttttttcc ttctttcact atactttttt cattatatct
     4621 agtctaacag aatcagcaaa taacagaaag aaggtgagac gaattcttaa aaaaagaatt
     4681 tgtctcacca atcctgctgt cagaaattag tttgcatttt ttccatgaca gttcttatat
     4741 tttttcccgc ttccacaagg acaaggatca tttcttccga ccttattcac atggattggc
     4801 tgttttttcg cgctagtatt actattttct ttggcatctc cgtcttctga gtgagaagct
     4861 gttccttgag aaacctgttc acgctgtacg ttttgacgga tctcagcttt cataaagatt
     4921 cgagtgactt catactcgat ggcgcctacc atatcttcaa acatcttata gccttctgtt
     4981 tgatattcga ctaatggatt gttctgtcca taagcacgta agccgattga ctgacgtaat
     5041 tgatccattg catcaatatg gtctgtccat ttagagtcca caacacgcaa gatcacgact
     5101 ttttcaaatt ccaacagttg ctctggacca tttagttgag ccgctttggt attaaatact
     5161 tcttgtgctc gttcgtttag atactctttg atctcctgag ctgtttttcc ttcgatatct
     5221 gcaagagtaa tgctatcttc atggacaaga gtactgccgg caaaatcgat gatactttcc
     5281 aaattccaat tttctttctc taattgtgta tgcgcatcta ctacacgact gattgtccgt
     5341 ttgaccatgt tcattagtgg cttggataag cttttttctt ccatgattac ttcttgtcgc
     5401 tgtttataga ttacttcacg ttgttcacgc atcacatcat cgtattgtaa gacgttttta
     5461 cgtgtgtcat agttatttcc ttctacacgt ttttgtgcag attctacttg tttcgtcagc
     5521 atcttgcttt ggataacagc atcttcctca ccgatcttca tccgatctaa gaaagctttg
     5581 attcgttctg aaccgaatcg tttcatcaaa tcatcttcca atgacaagta gaactgtgac
     5641 ataccagggt ctccttgacg tcctgcacgt cctcgaagct ggttatcgat acgacgagat
     5701 tcatgtcgtt ctgtcccgat aactgctaga ccaccaagtt ctcttacccc aagtcctaac
     5761 ttgatatctg ttccccgacc agccatgttg gtagcaatcg tcacagcacc tttttgacca
     5821 gcattcatga tgatctctgc ttctttaaag tgatttttcg cattcagcac ttcatgagga
     5881 atacgttctt gattcagcat atctgataat aactcagatg tttcaaccgc aacagttccg
     5941 actaacacag gttgaccttt gcggtagcga tctttgatat cttgcacgac agcgtggaac
     6001 ttgctttgta atgttgggta aagcaaatct ggacgatcat cacggataac cggacgattt
     6061 gtcggaatct ggtatacttg gatgttataa atttctctga attcttcttc ttctgtttta
     6121 gctgtaccag tcattcccgc aagtttcttg tacatacgga aatagttctg gaaagtgatc
     6181 gtcgccatgg ttttcgtttc gtcttcgatc tctacgcctt ctttagcttc gattgcttgg
     6241 tgaagtccat ctgaataacg gcgtccatcc atgatccgac cagtgaactg gtcaacaatc
     6301 agtacttttc cgtcttggac aacataatcg atatctaaaa gcatgatata gttggctctc
     6361 aatgcttgat ccaagtgatg ggtcaatgca gtattttcaa tgtcgtataa gttatccaaa
     6421 ccgaatgttt cttctgcttt ttcgatccct gcttcggtta aactgattgt ttttgattgt
     6481 acatcgattt tataatcttc ttcttctttc aaacgtttca cgaagttgtc tgctcttgta
     6541 tataaagctg tggatttttc tgcttgccct gaaatgatca gcggcgttct cgcttcatca
     6601 atcaagattg agtcaacttc atcgacaatg gcataattca gcggacgttg taccatttga
     6661 tggcggtaaa cgaccatatt gtctctcaag tagtcaaatc ctaattcatt attcgtacta
     6721 taagtgatat cacaattata agcctcgcgt ttttcatccg aagattttga gttgatattc
     6781 aaccccactg ttaatcctaa gaaattataa agttcgccca tttccgtcga gtcacgggtt
     6841 gctaaatatt cattgacggt aactacgtgt accccttcgc cagtcagagc atttagatat
     6901 actggcatcg ttgcggtcaa ggttttacct tcaccagtac gcatttcagg gatatttcca
     6961 tcgtgaagca cgattccacc catcaattgt acgtggtatg gatataaacc taatacacgt
     7021 tttgctgctt ctcggacgac tgcaaaagct tccggaagta attgatctaa tgtttctcct
     7081 ttttggtatc tgcccttgaa ttcagcagtt tttgcctgta attgttcatc agttaatgct
     7141 gccatttggt cagcgtatgc ttcaactttt tttgcaatgc tatctaaacg ttttagttct
     7201 ttcttatcgt tttcgataag tgaacgaatc aaattggcca tgcgttgtgt aatctccttt
     7261 tccttctgtg tattatccag actatgtaga ctttactttt cattttatca ttagttatag
     7321 ataattgaaa tatcttaagc gtattaaagt aagaacttca taatttattc atatttcatc
     7381 taaaagagga ggaaactcaa aaaaacttgc ggcagctgtc agtagctgct gcaagttttc
     7441 gttgttattt aagtaaggct gatcagtctg tttcaatcag tccgtatttt ccatctttac
     7501 ggcggtaaac gatacttgta ccatttgttt ctgcatcttc aaaaataaag aagttatgtc
     7561 ccagcatgtt catttgtaag actgcttctt cactatccat tggtttcaat gaaaggcgtt
     7621 tcgtacgtac gatatctaat tcagaatcat tttcgttttc gtcctcttca ttgaataaag
     7681 cagcagcatc tgcagtattc atgcccgttt cacgggattt acgattaatt tttgttttga
     7741 atttgcggat ttggcgttcc aatttgtcaa caaccaaatc gatacttgcg tataaatctg
     7801 gagaagtttc ttcggcacgt aatacaagat aaggtaatgg gatcgttact tccacttttg
     7861 ctgttttttc tgtataaact tttaagttca catgcgccgt tgcatcgggt gcgtcactga
     7921 aatagcgttc tagtttgcct acttttttct caacgtagtc gcgaatcgct tcagttactt
     7981 cgatattttc gccgcgtaca ttatatctaa acataaaaat tacccctttc gtctaccaca
     8041 atcaagaagg agaaggacca ctctttccct cttcttaatt ctattatatc gaactctagg
     8101 ttatttgcaa attaaatcgc tatcaaattt ccacatctga ttcatcttgc caaagaaatg
     8161 gttcggatga ttttcggctg tgcgttctgc aagcaatctg ctgcatgaaa aagcgtctgt
     8221 cctgttgtat agacatcgtc aataatcaat attttt
//
LOCUS       DAZULG010000111         8226 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000111 DAZULG010000000
VERSION     DAZULG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.114"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG9CY_002337"
     CDS             476..1339
                     /locus_tag="ACG9CY_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4938820.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG9CY_002338"
     CDS             complement(1468..3090)
                     /locus_tag="ACG9CY_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4938821.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG9CY_002339"
     CDS             3339..3860
                     /locus_tag="ACG9CY_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4938822.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG9CY_002340"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG9CY_002340"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS4938823.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG9CY_002341"
     CDS             5941..6759
                     /locus_tag="ACG9CY_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS4938824.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG9CY_002342"
     CDS             complement(6862..7059)
                     /locus_tag="ACG9CY_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938825.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG9CY_002343"
     CDS             7228..7806
                     /locus_tag="ACG9CY_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4938826.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG9CY_002344"
     CDS             7901..8038
                     /locus_tag="ACG9CY_002344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938827.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2472 a         1481 c         1620 g         2653 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacat cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaa
//
LOCUS       DAZULG010000112         8124 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000112 DAZULG010000000
VERSION     DAZULG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.116"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..765)
                     /locus_tag="ACG9CY_002345"
     CDS             complement(13..765)
                     /locus_tag="ACG9CY_002345"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4938828.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1129..3795)
                     /locus_tag="ACG9CY_002346"
     CDS             complement(1129..3795)
                     /locus_tag="ACG9CY_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4938829.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4136..5587
                     /gene="cls"
                     /locus_tag="ACG9CY_002347"
     CDS             4136..5587
                     /gene="cls"
                     /locus_tag="ACG9CY_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4938830.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(5916..6731)
                     /gene="thiD"
                     /locus_tag="ACG9CY_002348"
     CDS             complement(5916..6731)
                     /gene="thiD"
                     /locus_tag="ACG9CY_002348"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS4938831.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            7025..7300
                     /locus_tag="ACG9CY_002349"
     CDS             7025..7300
                     /locus_tag="ACG9CY_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938832.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            7727..>8124
                     /locus_tag="ACG9CY_002350"
     CDS             7727..>8124
                     /locus_tag="ACG9CY_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4938833.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWV"
BASE COUNT         2422 a         1677 c         1373 g         2652 t
ORIGIN      
        1 aaaaagaacc cttcacgagt cgtgaaaggt tcttttttta tctgcggacc aaaggagcag
       61 tctataggtc agcttagtcg tactaaggat ttctcgtttt tcactgaata gtttgcgaat
      121 gtacctggtc acaacaggat cttttgcgta cgcgtcatag tatagcttca tcaattcttc
      181 agtaattgat tcctgtgtaa aataagtaaa atcttttttc ttcactttga atccttcatc
      241 aattaagtat gtttcaaatt gatccattac aactggcgat gtattaggat cttcttctat
      301 cattcctagt tgcttttcga taggataaaa aacatcatcg attgattcca ctactctgat
      361 tatcacgcac catttgcttg actttttcat caatttttct aattgaataa gatgtaccaa
      421 tgctggattt gctgctgtaa aaaccatttc atatgtctca tttgattgga taaattcttc
      481 taacgactgt ttgataaagg aaacatttgg caactcattt ttttctgctt ctttttttgc
      541 attttctagc attcctgaag aaaaatcgat caaatcatag cgattaattt cttttgctaa
      601 ggaaagaaat ctgccagatc ctccagcaac gtctgctgct gtttcagcag gcaggacttt
      661 ctccgttttc agataatttt ttacatcttc agcaatagtt acttgcgatt cttgctggat
      721 ttcataatat tcttcagcaa attcatccca ttctttttca ttcatttgat ttcctctaat
      781 tttttgttat atgttttcac ttttcggaat aaatcattct cctcttttat ctattctatc
      841 tcttttgatg aataataaaa tgattggctt tccgtctatc ataaataaaa aaagggactg
      901 tgaccaaagt cgtgtcaaag tccctctttc cgaataaaca gtgtccaatc agatgaccct
      961 cagtattagc tcaaaatgct gattttacaa cttcttcctt aaacggtgtt ctcctgccaa
     1021 ctcttcggtc acaagtcaca atattcaaca aacatgagaa gctgtttgtt gaaattgcac
     1081 cttgtgactc agaattaaac ggcaggctca caacctcgtt gattcgtttt atttatttcc
     1141 ttgtactcgt acaatgatca atttagtgat ttccatcaaa acaattgtca caagtgcgat
     1201 tcccatacat gtaaagactt ctgtcaatcc aaatgctgat gggatagaga agacttcttt
     1261 cagtcctggg atcattgtta agcagtaaag cgcgctacat acaactacag ctagtaagac
     1321 acttctgtta gagaagaacc ctgcttggat agctgtttgt gtgtttgaac gtgctgggaa
     1381 tgtttgcaat gtccgacacc aaatcaatgt tgaaaatgac atggctacac cgaattcagg
     1441 tgaatgctgc atgccaagcc attgagcaaa gataacagca atcccaatca gcacaccacg
     1501 atacgttaca gaaatcaatg ttttgtccgt aaagattcct gaatttggat ctcttggcgg
     1561 gcggttcatc gtagcaggtt cagctttttc tgtacctaat gcaattgctg gcagtgaatc
     1621 attgactaaa ttgatgaaca acagttgtaa tgctgtaaat ggattggacc agtcagcaac
     1681 taaggcaaag atgatcgcaa tgatcgcacc taaatttcct gcaaatagat aagaaatagc
     1741 tttcttgata ttatcaaaca cattacgtcc tacttctact gcgctgacaa tagaagcaaa
     1801 gttatcatcc gtcaatacca tcgctgctgc atcttttgcc acatctgtac ccgttcccat
     1861 agcaattccg atgtctgctt gtttcaatgc tggtgcgtca tttacaccat cacctgtcat
     1921 agctgagata tgacctttat tttgccaagc acgaacgata cgaattttat tctcaggtga
     1981 aacacgtgca taaacagtga ctttttctaa aacttcgttt agttgatctt ctgtcaattc
     2041 atctagttcc gtcccggtca aagccaggtt tccttcttca aaaatcccga tttgttttgc
     2101 gatagcaaca gctgttgttt tgtggtcacc agtaatcatg attgttttga ttcctgcact
     2161 tcttgcttct gttaccgcct gagtcacttc agcacgtgga ggatcgatca tagcaagcaa
     2221 accaacaagg atcaaatcgt cttcatcttc aaatgataaa tcaaaattat caagtggacg
     2281 gtaagcaaaa gctagtacac gtaatgcgcg tgttgaaaaa gcttcatttt gctcttggaa
     2341 ttttttcatc aattcatccg tcatcggaac gacttcacca tttagcaata cttttttact
     2401 gcgttggaag acgatatctg gtccgccttt aacaaacaag taatttttgt tatcaattgt
     2461 gtgtccagta gacattaatt ttcgatctga gtcaaaagga agttctgctt gtcttgggta
     2521 gttgtttcgg atttcctgat aaggctggtt taatttttca ctaaagtcga taaacgctac
     2581 ttccgtaggg tcgcccaatt tagtaccgtc ttcactgatc gatgcatcat tcgctaaaac
     2641 actgatttcg atcaagcgac gttcgtcagc tgtccatttt gaagggtcat ttgtgaaatc
     2701 cccagtatcg ccattaccta aaaagtagtc aacaactgtc attttgtttt gtgtcaatgt
     2761 accagtctta tctgtacaga tgatacttgt cgaaccaagt gtttcaactg ctggaagctt
     2821 ccgaataatg gcatgacgtt ttgccatttt attcgttccc aaagataaca cgattgttac
     2881 gatagactgt aatgcttctg gaatagcagc tacggctacg gctacggcaa acatcactgc
     2941 gctgacgata tctgcagtca tatctcctgt accttctcca agataaatac gaaacagttg
     3001 gatacctaaa atcaataaag acaatgccaa aatagccaaa cttagttttt tactgaattt
     3061 atccagacct cgttgtaacg gagtgacttg ctcagttgtt gattccaaaa ggccagcaac
     3121 ttgtccgatc tccgtattat tcccagttga agtcactaag aatttaccgc gtccgtaagt
     3181 tacgatcgtt ccactgaata ccatatttgt acgatctccg attggtaccg tacttgtgat
     3241 atccgttact tctttttctg cagaagtcga ctctcctgtc agcatacctt catctacttt
     3301 taatgatccc gcatctagta aacgtccatc agcaggtaca taatcgcctg cgtctaacaa
     3361 cacgatatct cctaccacaa gttcgtttgc cggaatcgtt ttttccttac catcacgaat
     3421 cactcgagca tcgggcgctg aaaggttttt caacgcttct aaagaacctt ctgctttttt
     3481 tgtttggaca acactgatta tagaattcaa catcaatact gcaaaaataa ctaagaattc
     3541 gatgtagtca cccatgacca tctgaatcag tgcgacaatc agcaatacga taaccattgc
     3601 atctttgaaa gtttctaaga acaatttcca agttggcttc ttcggctttt cttttagctg
     3661 gttcaagcca tctctttcta atttttcctt cgcttgcgtg gtgctcaatc catcggttga
     3721 gctctgcgtt tgctgcatca acgagtcgat cgtttcttta tagaccgctt gttgtgcttg
     3781 ttccttgttt tccaaatttc atcccccatt ttttgatctt ggccctagtc ctgtctgaaa
     3841 aaaaagagac ctaagacaaa tacgcatttg tcttaggtct caccatttca tacaatacca
     3901 gaaaaccatt tggttaactt actgttgata ttatcgcaac aaacgttgcc ggttactccc
     3961 ctaaggagat attcaactgt actaatatcc taccttatta acctacaaaa agcaactact
     4021 ttagcccgat ttacctacat tttcatgaaa ggagtgaaaa gtatattcac cttggtaggc
     4081 ttctttcttc tatcatgcta tactaatgtt attgtatgta aggaggaaca tttttgtggt
     4141 atctagtatt ataaccgccc tttatctatt aaatgcactt attgctttgg tggctatttt
     4201 gatcaaaccc cgagatgtag cagccatttg ggcatggctc ttagtattgt ttgcccttcc
     4261 tggggtgggc tttgtattat atttattttt cggacgtgga ttaacggata agaaaaaatt
     4321 ctatctccga caaagtgact tgaaagaatt agaaaacttt cagtctttta gaggagatac
     4381 cattgaacat tacgatcctg acatgggcga taaagacaaa cagcaatttg ttgacttctt
     4441 ctcttcatta aatcgtatgc cgctgacaag aatgaattct gtcactcttc tcacagacgg
     4501 acaagagaaa ttggattcac tgcttcaaga tctaaaaaaa gccaaacatt cgatccatat
     4561 cgaatattac gcatttgtga cagataatat cggccagcaa gtcttacatg ttttagaaga
     4621 aaaagccgca gaaggcgtgg aagttcgaat attatatgat gcatttggct ctcatggcac
     4681 aaaagcaaaa gatttcaatc gtctaatcaa aaatggtgga catgtccata catttgttac
     4741 ctcacaaagg gcattacttc gtttccgatt gaattaccat gatcaccgaa aaatcgttgt
     4801 gatcgatgga aagattagtt ataccggtgg tttcaatatt gccaatcaat atgtaaatac
     4861 aacaaaaaaa ttcggctatt ggcgcgatac gcatatacgg attttcggtg ccgcttcttc
     4921 tttgctccag cttcgcttct taacagactg gaacgtctcg gtacctgaag aaaaaaaggt
     4981 cggctatcat ttgaattatt tctttaaaaa agcagatcga gatgaatcta agcttgctga
     5041 tacatccatc cagcttgttt caagcggacc gaataacgaa agggaacaaa tcaagctttc
     5101 atttatcaaa ttgattactt ctgctaaaaa acgtgtttgg atacagacac cttaccttgt
     5161 tcctgatgaa agtgtcattg ctgctttaaa aatcgcaact gcctctggtg tagatgtgaa
     5221 aatcatgatt cccaacaaac cggatcatcc ttttatttat cgagcaacac aatattatgc
     5281 tcggcagctg atcaaggaaa atgtacaaat ccttgtctat gagaacggct tcctccatgc
     5341 aaaaacattg ataatggatg atgaaatctg catggtaggt tcagcaaatc aagatattcg
     5401 tagctaccga ttgaattttg aaacaagtgc tgtcatttac gatcctgagt ttttagaaga
     5461 acttgctact cagttcaaag aagatgagac acattgttca tccatgacaa ctgaaacagt
     5521 caaggaaatg tctaactggc tattattcaa gcaacaaatt tctcgattat tttctccaat
     5581 cctataattt ttttattcaa gtagagaaaa atatactaag attaaaggga ctgtgacaaa
     5641 aatcttgtca cagtcccttc ttccgaacaa acggtgttct cctgccaact cctcggtcac
     5701 aagtcacaat attcaacaaa catgagaagc tgttttttga acttgttcct tgtgactcgg
     5761 agctggacgg caggctcacg acctctttct taaacggtgt tcaaaagctt gcatctgcgg
     5821 taataagtcc aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac
     5881 ttgatgcagg gcaacttttt cacaacttct ttcttttact ctaattcacc agctgcccaa
     5941 tgattgatgg ggccatattt gtgccctaca tttatttctt cacgaattgc acggtttaca
     6001 tagtcttttg ccaatcggat cgcttgttca actgtttttc ctttcgctaa ctcagctgca
     6061 atacatgcag atagcgaatc gccagttccg ttgatccggt cggtaggata aaatggtgca
     6121 tttaaccaaa atgattttcc tgattcgaac aatacgtagt cattgactgt ttcttgctgc
     6181 gcatttttag catgatgtcc tttgatcata acgttttttg cacccatatc ttgcagttct
     6241 tttgctgctt gcacaaaatc ttcttcagct gctaatgtcc gtccagtcag cttttctgct
     6301 tcataaaaat taggtgtcag tacttcggcc aaagggacaa gcttttcttt caagctttgc
     6361 aaagcagatt cttctagtaa aagatttcca tgtttcgtta cgattacggg atccacgact
     6421 aatggtccaa aatcatattt ttggtaattt tttacaactg tctcaatcaa ctttgaatct
     6481 gctagcatac ctgttttagc tgcacgtatt tgaaagtcct tcgcaatcag ttcaaattct
     6541 ttgtcgataa aatctgttgg caatgtcaca cttgctccga ttccgtaaga attacctgca
     6601 acacatgcgg tcatgacaga aacaccgtaa acatttcttg taaagaatgt atgcatatct
     6661 gcttgcatcc ctgcgcttcc atcgctatct gatcctgcga tcgtcattgc ttgtacatac
     6721 tgatcttcca tcaaatcgat tcctccattt ttataaaaag atttttcata atactgttac
     6781 tgtttctatc atatgttttt caaagtatcc ctcagtaaat ataccactac cttttcttgg
     6841 taatcaaatt tatttgaaaa aacatttgca ataaatcaga aaatagtggt cgtttatttg
     6901 tattcgctat tctaataata aataaccact atttgattta tatatatttc agtatccttc
     6961 ttgttttcat ataaccaaag gcgctatgat ttccatatga aaaaaataaa gtcaggtgaa
     7021 aaaaatgaaa cttgaggata aaaaagtcat tttgaagaaa atcaaacgac taccagacca
     7081 aatagatggc tatacgatct gttctttcaa aaaagacagg agtataaaag cttatacgca
     7141 aaacacagaa aaaggaataa tctatttcct acatgaagaa ggttacgata cccaaacctt
     7201 tatgtttact gaaaaaaaag aatttcataa acagatgaaa aaactgattg aaaaagaatt
     7261 tccaagaagt cataaactct atgtgaatca gcaattttaa cttctaactg ttctttatta
     7321 attaaaaatg ttctgtaata aaataaaact ttataacaac tataacaaaa ccgaatgcaa
     7381 aacagaagtt ctataagtta taatcatagt tgttaggtga ttcatctagt aaaaacacca
     7441 gatttcaaat ttcacacaat ttaaactaga tcaatcatca accttcttca aattgttaga
     7501 accctttccc gaggagctgc agcataagca aaaaaacaaa caggaagcca aatggctaag
     7561 acattttttc tattagtcta gttgcttctc gtcgcaagtt taagacttta gaaagttatt
     7621 tctaatgatg tttgggttgt tttgatcaat gcttatgcac agctccttac ctattattta
     7681 tctcaacaga tctaacaaat taatggacta tttaaggagt ttttttatgc ttacgatcta
     7741 ttcttctcgc acacatagtt accatctgat ggcaaactgg ctgaaagacc atcaaatgcc
     7801 ttacagagaa cgaattttca cacctaaaca gcctttaaca aggaaagaga tattttattt
     7861 attgtcatta acagaaaacg gatttgacga tctattagct aaacgctcga ttgattataa
     7921 aaatttgaaa gaccaaatcg accaatgtac gacctcagag cttgtcgatc tgatcgtctc
     7981 gcattttact ttgttacaac gtcccatcat tactgacgga aagaaattat tagtcggatt
     8041 caatgaagat agcatgcgtc tttttatccc tgtacaatgg cgcagaagtc aactgaaatc
     8101 tgtctataac gattttacat gggt
//
LOCUS       DAZULG010000113         8093 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000113 DAZULG010000000
VERSION     DAZULG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.117"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            382..570
                     /locus_tag="ACG9CY_002351"
     CDS             382..570
                     /locus_tag="ACG9CY_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS4938834.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            648..1661
                     /locus_tag="ACG9CY_002352"
     CDS             648..1661
                     /locus_tag="ACG9CY_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4938835.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1728..2762)
                     /locus_tag="ACG9CY_002353"
     CDS             complement(1728..2762)
                     /locus_tag="ACG9CY_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4938836.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2877..5102
                     /locus_tag="ACG9CY_002354"
     CDS             2877..5102
                     /locus_tag="ACG9CY_002354"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4938837.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5120..7003
                     /locus_tag="ACG9CY_002355"
     CDS             5120..7003
                     /locus_tag="ACG9CY_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS4938838.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6984..7958
                     /locus_tag="ACG9CY_002356"
     CDS             6984..7958
                     /locus_tag="ACG9CY_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS4938839.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2613 a         1475 c         1708 g         2297 t
ORIGIN      
        1 gttagtctac attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa
       61 tctgggtcag aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact
      121 gattgatcaa aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg
      181 tttgacgcgg aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga
      241 cccattattt tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg
      301 ctgggagttt tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg
      361 actgtaacga aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa
      421 gcgacacgat atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca
      481 acagaagaag aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca
      541 ttgcagaaga agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac
      601 actatggtat aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa
      661 ttcttaaatt tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc
      721 ttgccttcca gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata
      781 ctgttcaaat cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa
      841 cagatttgac gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa
      901 acagcaaggg gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag
      961 ataaatcggg tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg
     1021 ttgcgatgaa ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga
     1081 atgaactgat caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac
     1141 aattattttt tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac
     1201 aaacgaatga tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc
     1261 tgaaaggaac catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt
     1321 cggatgatcg atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa
     1381 aagattggaa aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg
     1441 aatttccgcc aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag
     1501 cattagaaaa tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata
     1561 caaaaaaaga gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt
     1621 gttaccagca gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt
     1681 ttcgttttga gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt
     1741 gttcttggga taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt
     1801 tcgtcccgta taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat
     1861 ttcgcattga tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta
     1921 tcaaagcttg ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga
     1981 tagacaccaa ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca
     2041 ttttctaata gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga
     2101 taagacatat atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc
     2161 ttttcattta tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg
     2221 atataggcta ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc
     2281 cgttcaagat ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg
     2341 actatttgca tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca
     2401 acgatcaccg cacctaactg atctagatct tcccattgtt taggattatc agatagatta
     2461 tagattcgat tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa
     2521 tgtttgtaat agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc
     2581 gaaggcattc tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct
     2641 gcctctacta tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac
     2701 acacgggaaa ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc
     2761 atttttacac actcctcaaa aaaataaaaa atttttattg acaaaattgt ttagtaaaca
     2821 gtatacagga attataaaaa aaagtaaacg gtttccgttt tgttgggagt gaaatgatgg
     2881 atattacgca tagaaaaaca gataaaaatt tcatcttatt tgatgaaaag agccgagtct
     2941 tccatttacg aaatacattt ctttcttatc tgataaagat agaagaaagc aatgtattgg
     3001 cgcatattta ttttggcaaa ccagtcaagc agtacaaaga caataaaaat tatccccgaa
     3061 gagatcgtgg attttcagga aatgttccct taaatcctga ccggtctctg tcgaaagata
     3121 cactaccgca agaatactct tctcatggca gcatggactt tcgaacgccc gcatcgatca
     3181 ttcagcgcaa gaatggatca gacttactgg atctgagata tgacagccat tatattacag
     3241 atgggaagcc agagatcgaa ggcttgcctc aaacttatgt gatggacaag tcagaggcac
     3301 aaacgttggt gatttcactt aaggatagag aaacagcgat ttatttcgat ttgttctata
     3361 ccatatttac tgatagagca gtgatcacac gatctgtaaa gataagaaat gaaaccggag
     3421 aaacaatcaa actagaaaaa gcggctagtt tccagctaga ctttgcccat actaggcgat
     3481 ttgatgaagt cattgctttg ccaggagcac atgtcaatga aagacagatt tcacggcaat
     3541 ctgttttatc tggaacaaaa gtatttgaaa gccgtcgagg aacgagcagt caccatatga
     3601 ataactttat tgctttagtt catcatcata cgacagaaaa tactggagaa gcgatcggac
     3661 ttcaatttgt ttattccggc aaccattctt tcgaacttga aaaagatcag atcaatcaat
     3721 tgagagtcgt cggtgggatc aacagccatc gtttttcgtg ggaattgaat gcaggccagt
     3781 cttttcaaac acctgaaatg atcctttctt actcaagtca gggattgaac aaaatgagtc
     3841 agatccacca tgagttgcta cgtgaaagaa tcgcaagagg tcgtcatcaa tttgctgaac
     3901 ggccgattct ggtcaataac tgggaagcca cttactttga cttcaatagt gaaaaaatca
     3961 aggcgatcat tgatgaagca aaggagctag gcatcgaaat gttcgtatta gatgatggct
     4021 ggtttggaaa aagagatgcg gacaattctt cattaggaga ctggttcgag tatgaaggca
     4081 agttgacaaa tggattacgt gaaatagcag attatgctca tagcaaagga ttgaaatttg
     4141 gtttatggtt tgaacctgaa atgatctcgg ttgattctga actgtatcga acacatcctg
     4201 attttcttat gcaggagcca ggtagaatgc cttctgcttc cagatcacaa catgtactgg
     4261 attttacaag gttagatgtt cggcagacga ttgagaaaca aatgagaaag attttagata
     4321 cgattcctct tgactacatc aaatgggata tgaaccgttc gctttcagat gtctattcga
     4381 ttacccttga cccacagaga caaggagaag tagcgcatcg ttatatgctc gggctttatg
     4441 aactacttga acatttgaca actgattatc cggaaatctt atgggaaggc tgttctggtg
     4501 gaggtggacg gtttgatgcc gggtttattt attatatgcc gcagtcatgg acaagtgata
     4561 acacggatgc agtggagcgt atgaaaatcc aatatggaac aagccttgct tatccgattt
     4621 cgtccatcac agctcatgta tcagctgtac caaatcatca gaccgacaga tctacttcgt
     4681 tgaaaacaag aggagaaaca gctatgagcg ctgtttttgg ttatgaactg gatttgacaa
     4741 agctgtctcc tgaagaaaaa aagcaagtaa aagaccaaat tatatcctat caaacgattc
     4801 gtccggtgat ccagtatggc cattattatc gtttggcgag tccttttgaa gaaaatattg
     4861 ccgcatggat gtttgtgtct cctaaacaag atgaagctat tgtgttttta gggaggatac
     4921 tagcttcagc tcaaccagcg ttccatgaag tatatctgat ggggttagat gatgaggcac
     4981 tttatcagga acagacctcg aagcggatat tttcgggggc cgaattgatg acagttggac
     5041 tttacttccc cgattttcaa ggtgatttcc aaacagaact gcttcatttc aaaaagttat
     5101 gagagagaag gaaaaaagta tgaaagcaaa tataaaatgg ttagatgatc cacaagtttt
     5161 tagagtgaac caatcaaatg cacatagcga tcatgtttgt tatgcaacaa gggaagaagt
     5221 agaaaccaca tcctccctta aacagtcttt aaatggatca tggcgttttt gttattcgcc
     5281 aaatccttcg gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga
     5341 tgaaatcact gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa
     5401 tacgatgtat ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga
     5461 gaaagaggcg gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac
     5521 gacttttcga ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt
     5581 agaacaagca atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt
     5641 cactccgtct gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga
     5701 ggtttacaaa agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg
     5761 gatcttcaga gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat
     5821 caaacctcgc ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga
     5881 gcaagtagcc aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat
     5941 ctatcaacag caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa
     6001 tgtggcactg tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga
     6061 cgaagaaaaa agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat
     6121 caaagataaa atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca
     6181 tgagtggcat ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc
     6241 tatcataaaa aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac
     6301 ttggtattat ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc
     6361 ccatggctca tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt
     6421 acctcagtgg caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa
     6481 ccatccaagt attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg
     6541 acaaatgaac gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa
     6601 ttttccaaat ccagcattcg aagattcgat ttcagatgta aaaagctgca tgtatgcgcc
     6661 accagaagag atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga
     6721 atatatgcat gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga
     6781 tcgttatgaa atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt
     6841 gactgatcca atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc
     6901 aagtgactat gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc
     6961 catgcaggag gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg
     7021 aaaaagttgc aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt
     7081 atattttttc ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc
     7141 tataccggga aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg
     7201 gatttggatt taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa
     7261 tagaagtact ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat
     7321 attcaagtaa tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag
     7381 taccaaaaac ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca
     7441 atatgcatta ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca
     7501 ttatgccaac gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg
     7561 atcggatggc tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat
     7621 accttgttcc ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa
     7681 atcaaacgtt ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc
     7741 aggatacgcc atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa
     7801 cacatataga agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa
     7861 gaggagtcgg gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca
     7921 gcgaagagga ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga
     7981 aaaacggagt ttacaagaca aagctgcttt attctatatg tcatgaacaa gagagtaaaa
     8041 aagttctcag atacttttgt tcatgactat tttcttcaat aaaaccaatt gtt
//
LOCUS       DAZULG010000114         8092 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000114 DAZULG010000000
VERSION     DAZULG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.118"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2454)
                     /locus_tag="ACG9CY_002357"
     CDS             complement(<1..2454)
                     /locus_tag="ACG9CY_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938840.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTW"
     gene            complement(2438..2773)
                     /locus_tag="ACG9CY_002358"
     CDS             complement(2438..2773)
                     /locus_tag="ACG9CY_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938841.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(2787..3821)
                     /locus_tag="ACG9CY_002359"
     CDS             complement(2787..3821)
                     /locus_tag="ACG9CY_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4938842.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(3911..4591)
                     /locus_tag="ACG9CY_002360"
     CDS             complement(3911..4591)
                     /locus_tag="ACG9CY_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4938843.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(4761..5720)
                     /locus_tag="ACG9CY_002361"
     CDS             complement(4761..5720)
                     /locus_tag="ACG9CY_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4938844.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            6017..7336
                     /locus_tag="ACG9CY_002362"
     CDS             6017..7336
                     /locus_tag="ACG9CY_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFS4938845.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            7777..>8092
                     /locus_tag="ACG9CY_002363"
     CDS             7777..>8092
                     /locus_tag="ACG9CY_002363"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="malic enzyme; oxaloacetate-decarboxylating;
                     NAD-dependent; catalyzes the formation of pyruvate form
                     malate; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HFS4938846.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEI"
BASE COUNT         2364 a         1613 c         1486 g         2629 t
ORIGIN      
        1 ccatgtgacc atgccttgat acgttgcgcc aggttgagca ttcgtactgc tatttacctc
       61 taagagtaaa ccatcggtat cgggaaagtc aacgagaacc tctcctttag ctgttccatc
      121 actctcataa atcaacgtgt tttgaccttg ctgaatcaat tgctttgatc cagaatgatt
      181 gaagtataat tttgttccgc ttaattcttg accttgatca ttcttaaata aagaagtcaa
      241 tgtacctgtc agttgccaag gagacgtgct tggaagccga gtatcccgca catcaaaaga
      301 aaaatcgcct tttgattcat aataacttgt cttcaacggt ttggggagat cgttaccaaa
      361 tgtaaaatca tctggtacat taactaatgt taattcttgt ttggggacaa gaattgtttt
      421 agcagatgtt tggacaactg ggttaggaag cttagcacca tctgctaatg taatattgct
      481 ggaaagtaca gctgcctttg tcagtatgct gacggattgt aaagaagtat ctacagtatt
      541 taattgatag ttgtaagtaa gatgaatggt atctcctatt ttgctcaata gatctgctgg
      601 gacagaaact ccttgttgaa tgccggtatc tgcgatagtt gtaccattta attttaagga
      661 accattgata taagtagtat tttcaggaag tgagatcgtg atttttttgt cggttatcgc
      721 atccccagat tttgttaacg tgaatgtcac attttcttga atcgtatctc ctgatagagc
      781 agacagggga tctcctgtct caggttgtct gctaaggttt tttagctgac tatctgcact
      841 cacagatgga aaattagcca ctgttatttg aggaagagtg gtgattccag cttgttcaga
      901 ctgccatttg tacgcaaaag acggatacat gttagtaagt acatcgtcca ccaagcgata
      961 atcaaaataa tattgccctg aagccaatgc aggtagtgta gaagtagctg tatttacacc
     1021 cccgttattt gattggatcg tttggaaaag ttgatctgtc catcctgtcg ctaatttttg
     1081 ggttcctttc gaataaagcc tgtaatggat cgaacccttt tttagttcag ttgctgacca
     1141 attggccgtg acttggtacg atgcagaaga aaggagcttt ccccactgac tatcggctgt
     1201 taagtttgtt ggtaaaaccg ttccgtctag cttgtactgc tccaatacct ttaaagcaac
     1261 aggagaaagc aatccgcctt gcgcatcttt cccactgtac acgatattgg ctgtctgatt
     1321 caatccactt gtgcttgctg gagtatgagt cagtattgct ccttgtgtaa cgttttttcc
     1381 tgtaaaatag ttgactacgc gatcaaagcg actaaatggc aactgatcaa aacgtccttc
     1441 agtcagcccc atcgtcaatg tccgactatt ggtagagcct ggaaggttct ggttatttcc
     1501 atccacgtat tctgtggtat cagcaaatgt attatatttt cctgttgaat cacttggtga
     1561 gatgtaacta ttccacgtat ttatctctgc acttgcagaa aatgcatatg ctcctggatt
     1621 caagtttccg ccagttaatt taggataaaa tctacctgca gctggagcta aaatcgaatt
     1681 gaacatacga tcttggataa tattattcga atcataaaaa tcaacagaaa aaacggcctt
     1741 tttagacata gaaggttgta atttaccctt ggtcaaaatt ttgtttggac tcgtatcata
     1801 tttatctccg ttttctgtca tcttcgcgac attagctgta acatcaaatt gaagcgggat
     1861 atcgtccacg ttgaattcgc ctcgcgtaat aatagcaata atatctgtca atagagtatt
     1921 tgtatcccac gaccccatcc caaaacgcaa ggtatggctt cctgctgata ataaacttcc
     1981 gctagtaacc ttgatggggg caccggcaat atacaagtag gcgttgttcc agtcaattgc
     2041 ttttaataca tcactgcttg atacatgttc cggcaaattg atgatcgcca caaatgcaaa
     2101 tccttgtgca gtgatattca ccaaatcaag tacatttgtt agtttcacat ggctttccca
     2161 atgaaaacgg accgtgccac ctttccaagg atcatttgaa ccatcgttca aataagtagc
     2221 agtcaattta ggcaattggt tagccccagt tagttggaca agcttcccgc cattgactgc
     2281 actacgtcca ccatttggag gtacgatatt ttcatacgtg tatggatcat aagaaatggc
     2341 aggaggcgtt gctccaacag cttctgccga aggtgaaaag ataaatatac ttaaaacaaa
     2401 aagcgaagta aaaaagaagg agcgaaaaaa tgaaaattta ttttgtagca acatgttcat
     2461 cctccttttc ctttcttctc cataacaaca accacgcaag aagccataat tctataccaa
     2521 tgatcaaata aagaaaacgg atcttttgtt cattcgtctc cgggaatacg ccatgttgat
     2581 tagattcttt ataaatcggt agctgtgtct gctgttttga tgcgccattg actgaaccac
     2641 ttggatgcgg aattggactt agatcataaa atgtaatcgt acctgaactt ttggtatctg
     2701 cttggactgg ctcttgccag ctgaagaaca gcaaacttgc aataatccct cccaataaga
     2761 tcgatgtttt caacgaaatc acctcatcat gttgtttctc cttttttctt tttcttgtgg
     2821 acccacaaaa acagcaaaat caaccataac actagaactg cagcaaccaa tcctgcataa
     2881 agccatggga aagattgttc ttgtactgca aaaacggaat tttcatttac tttttgtgct
     2941 ttttcttttt tgatcgtaaa gttttgtgta aatcgccatt cattactgtc gcctgatttt
     3001 gctatgattt ctgcgcggta atcgccggct tttgctttat caccagctaa atcaagattc
     3061 aagtgaaaat tcgaatttgg tgcaactaaa aatgcattag acgtattttc aatataaagc
     3121 tgattttcat aaaagatctt gctcgtaaat atcaaattgc tgataatcct aggctgagga
     3181 ttttggaaac gaagctgaac agttggcttt gcgttgacct cgtccaaatc tcctgataaa
     3241 agtttcagat ccggttctat cgtctcttga ctttcttgta aaacgactgc gagattataa
     3301 gcaatacgat ttttgatttg tgctccttct gtagcatttt ctgtttcagc ttcttcttta
     3361 ggtgaaacat gaatacccgc taatacacgt cccttaaagc tttttgctgg gattttcact
     3421 tctataggta cagttgtttc tgcatgtgct gatacattga tttccttttt cgcaatgagc
     3481 aaaacgtcac ggacatcaaa cggcaatgtc tcatctaacg ttgggttttt ttgactataa
     3541 tcgatcgttc caccatcact cgttaccgcc ggattgacag agacctcaaa tgttcgttct
     3601 tcatctgaag aattgctgat aattacttct agtgtctgat tttgttccgg tgccactaat
     3661 aaatcaaaat aaccgattgc tttatctatt tggttctctg gaaataccgg tttgattcca
     3721 aagtctcctg ctgaatcctt tgcttggctg attgacggaa aaatgaaaaa tagacttaaa
     3781 atagaaaaaa acgaaacaaa cagtcgttta ctttgattca ttgtccacca catcctttca
     3841 acaacaactg cggtaaaaaa tccttcacct atagtatttt caccgcagtt tgttgaattt
     3901 atttcatact ctatgctact gggccagcca cgaggttcca agtcaaatca gccgtgaatg
     3961 ttccagctgc tgctttcggc actttcagtg tgatatttgc tggatcataa acagaaagcc
     4021 atgttcccag accttgatct tttgctgctg taaatattgg tgtcgctgca cttgctttac
     4081 tcaacccagt tacagtttga ctagttggtc cagtggattc gttatctgac gtacttttaa
     4141 cagtggatgt tccaaaatct aattcagcgc cttgcaaaac ttgtgaaccg tttacaaaat
     4201 ctgaaaccga aactgtcacg ttccaccctt gtgcttggcc atctgcacct acacctcgac
     4261 gatctgatac ttggagataa ggtgtatcgt tttttgaagc gtctacttga tacgtttttg
     4321 ttccgctttc aatctcgtgt gtaccaaatg gcaattcggg aactacatcc agtgttaaag
     4381 gacctgtctg gccagttgct ggattttccg gatctgctgg ctctactggt tttgaaggat
     4441 cagtagggtt aagaggctcc acggtatttg ttggttcagt aaaggtaata tctccttttg
     4501 aagtcgcttg accctcagca aaagctggtg ttgccaagcc gcctagaaga agtgttgaaa
     4561 gtaataatgt cccaatcatg ttctttttca taataaatca ctcctttttc ttagtgacct
     4621 aagtatagta gagacagtat gcaaaaaatc ctcatgaatt catccgctta cttgaaatta
     4681 tgaccctttt tgtataaaag agaaaaaaac aataaaatcg tttacatgaa tattcttcct
     4741 tttttattaa gtttcgtttg tcaaattaag ttagacaaat catcttttgt gacgtaactc
     4801 aaaaatactt ccattggtgt ccgatagtgt aatgattttc taggaatatg atttcgtttc
     4861 gatgccaccg attggataaa ggtttcagat acaccattaa aatccatagc tttatacaaa
     4921 ccatctttac gtaataagcc atttgaatgt tcattcaatc ctcgttgaga aggtgttcct
     4981 ggatccgcaa aataaattga aatatcgttg gtattactaa tctttttcca atttgagaat
     5041 tcttttccac aatcaaatgt gatggattta aataagtttt ttgggacaca acgaaaccat
     5101 tgattcagcc tattttcaat gtctttagcc tgtctgcctt cgggtttcaa ggtaatgatg
     5161 actttagata gccgttcaac taatgtgatg accgcgcttt tatgttttcc acccacgata
     5221 gtgtcacctt ccaggtgacc aaattcatga tcaaactggg tatagtctgt ctttctgtct
     5281 cggattgacc ggcgaaaccc ttgttttcct cttttttctt tatgaccgtt tggttttctt
     5341 tttcccttca ttggtaaatc gtgagtgtca aaggttttat ctttaaacat tcgatacagt
     5401 gtgcgtaccg aacaagcaat aggaaactct gctcgaccaa taaggacatc aggagcccac
     5461 ccttgaacaa cctttttttg aatgtattcc tgttgctcat caggtaaaac aatcggatgt
     5521 cttccgcagt tacgtttgtt tttcttatat tgtttatagt agctcattgc gcttccacca
     5581 gatttaagaa aacgatagac cttataaata gtttgtcgag aacgttgtaa cagattggct
     5641 acttttgaaa caggttgtga ttgattgaaa taagcttcta tcattacgag ttcagttggt
     5701 gtaagatggt tataggtcat ttgtactcac tccttataat tctttcgtcg gaactatttt
     5761 gagtgtagca caaatgactt tttttagttg tctagcttaa ttttacaatc ggcgctatta
     5821 aaagtaaata tccattcact aataataaag atatatttac acctgaataa ttcatacttt
     5881 taattaaaac catgtgaaac tgcctcacgg cagcttacaa ttactttttc atcttcaccc
     5941 gttaagtgat gaaaaaagaa cccctttctg ctatggttaa agtgactaaa ccaaccaaaa
     6001 gaaaggagtt cttttcatga ctagcttaca caaaaaccaa gtaaaattca attcaaatat
     6061 cactatttct catacaggtg gtcaattatc gagtgattcg ggtctcgtcc tagtgaaaga
     6121 gctgatgaac atctttggtt tttctgaact ggctaagcaa cacattcaca ttgaagacga
     6181 acgagcgtat tttactcatg acaacttatc catacttgag cagttcatta tgcaattaat
     6241 tgctggttac tcagctgatt ctgcagctaa tctcctgaga caagatcctg tgtttaaagc
     6301 tgttttagat aggaaagaac tcgcttctca atcttcactt tcccgatttt tagatcgact
     6361 atctgaggag aatatccatg aacttcaagc tttgaaccaa gaacttatcg ataaagcacg
     6421 cctcatccgt aatgatacgg aattaatcat tgatttagat tcgacccatt cagatacatt
     6481 tggtcaccaa gaacaaacga attataatac acactaccaa acctatggtt accatccatt
     6541 ggtagctttt gacggattga ctggtgactt cttaaaagct gaactacgtt caggaaatca
     6601 atatacatca aaaggcgtaa aggagtttct cacaccttta ttagagcact ataatcactc
     6661 tctaccaaac actgacatct tggttcgtgg agacagcggg ttcgctacac ctggtgtgta
     6721 tgattcgtgt gaatcaaaaa agagtcatta tgttattcga ctgaagaata atcgtagact
     6781 aggtcaaata gctgagaaat cagtacttta tggcgataat caaaagtggg aagaacgaga
     6841 agttcagtac ttctcgacca cttatcaagc acaatcctgg tcacaaagtc gtcgcgtgtg
     6901 tatacgctca acacgtgaag cgggcgaact actctttcga catgaattta tcgtgacgaa
     6961 tctatcagaa aatgtttctc ctgataccat cttttctatc tatgccaaac gtggcacaat
     7021 ggagaacttc attaaagaag cgaaagctgg cttttacttt gacaaaacag acagtcctcg
     7081 ctttttggag aatcatgtcc gaatgatgct cagcttgata gcttacaact tagtcaactt
     7141 cttaagaacg attggctttg aagaagtcca aaagggaatg accattcatt ctattcgatt
     7201 gaagtttctg aaagttgctg ggaaattagt ccaaacgggt agacgagtct atctcaaatt
     7261 atctagttat catgtgtatc agaatgaatt ctacagggtc tttgctcgcc tgaggcgagc
     7321 cagtcaatgg atctaatcca acaatctaac gattttttta ctgggaagtt atccagggag
     7381 aagtatgctc aaaatgcctt acacccaaaa tttattcctc aatatttttg aacagtgaat
     7441 gtttgaagta actttttagt caaaatcagt tgatagggat attagtgata catatttcaa
     7501 taaaatgtgc caaagaatta aagctatgaa ttattcagga ttctatttat ctatctaaca
     7561 tatcctttat tcttctactt ttctttcttt agcatataag agatacgcat agtaatcaag
     7621 caaggattgt tgatataaaa agaacaaata ccgctatttt gatttctttt gactctatta
     7681 ctcatataaa taaaatttat tatacctgct ctttttgact atatcctcat ctgactccta
     7741 tacttcgttt gtattaaaac ttaggaggaa tcattcatgc ttacagggct aaacttatta
     7801 aataatcctt ttctaaataa aggaacagct tttacaaaag aagaacgggc aaaatacgga
     7861 atcactggta tgcttccaag tactgtccaa acattggaac aacaatctgt ccaagcttac
     7921 ggtcaatact taagcaaaca gaccgatttg gaaaaacgta tcttcttaat gaatctattc
     7981 aatacgaacc ggacattgtt ttataaattg atgggcgaac atttagttga attcatgcct
     8041 gtcgtttatg atcctgtcgt tgcggattca atcgaacaat acaacgaaat tt
//
LOCUS       DAZULG010000115         8036 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000115 DAZULG010000000
VERSION     DAZULG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.119"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..351
                     /locus_tag="ACG9CY_002364"
     CDS             <1..351
                     /locus_tag="ACG9CY_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFS4938847.1"
                     /translation="EQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKIKKMLLGKTIE
                     NYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQFIAADYDADLA
                     DQFINGNNFMPTTN"
     gene            complement(497..973)
                     /locus_tag="ACG9CY_002365"
     CDS             complement(497..973)
                     /locus_tag="ACG9CY_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS4938848.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(933..1289)
                     /locus_tag="ACG9CY_002366"
     CDS             complement(933..1289)
                     /locus_tag="ACG9CY_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938849.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(1267..1452)
                     /locus_tag="ACG9CY_002367"
     CDS             complement(1267..1452)
                     /locus_tag="ACG9CY_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938850.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(1453..1725)
                     /locus_tag="ACG9CY_002368"
     CDS             complement(1453..1725)
                     /locus_tag="ACG9CY_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFS4938851.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(1722..1922)
                     /locus_tag="ACG9CY_002369"
     CDS             complement(1722..1922)
                     /locus_tag="ACG9CY_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938852.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            2050..2262
                     /locus_tag="ACG9CY_002370"
     CDS             2050..2262
                     /locus_tag="ACG9CY_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938853.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(2295..2489)
                     /locus_tag="ACG9CY_002371"
     CDS             complement(2295..2489)
                     /locus_tag="ACG9CY_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938854.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(2502..2684)
                     /locus_tag="ACG9CY_002372"
     CDS             complement(2502..2684)
                     /locus_tag="ACG9CY_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938855.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(2681..2821)
                     /locus_tag="ACG9CY_002373"
     CDS             complement(2681..2821)
                     /locus_tag="ACG9CY_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938856.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(3311..3676)
                     /locus_tag="ACG9CY_002374"
     CDS             complement(3311..3676)
                     /locus_tag="ACG9CY_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938857.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(3716..4018)
                     /locus_tag="ACG9CY_002375"
     CDS             complement(3716..4018)
                     /locus_tag="ACG9CY_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS4938858.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(4018..4317)
                     /locus_tag="ACG9CY_002376"
     CDS             complement(4018..4317)
                     /locus_tag="ACG9CY_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938859.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(4310..4474)
                     /locus_tag="ACG9CY_002377"
     CDS             complement(4310..4474)
                     /locus_tag="ACG9CY_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938860.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(4480..4749)
                     /locus_tag="ACG9CY_002378"
     CDS             complement(4480..4749)
                     /locus_tag="ACG9CY_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938861.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(4761..5510)
                     /locus_tag="ACG9CY_002379"
     CDS             complement(4761..5510)
                     /locus_tag="ACG9CY_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938862.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(5507..6199)
                     /locus_tag="ACG9CY_002380"
     CDS             complement(5507..6199)
                     /locus_tag="ACG9CY_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4938863.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(6205..6876)
                     /locus_tag="ACG9CY_002381"
     CDS             complement(6205..6876)
                     /locus_tag="ACG9CY_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4938864.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(6869..7210)
                     /locus_tag="ACG9CY_002382"
     CDS             complement(6869..7210)
                     /locus_tag="ACG9CY_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938865.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(7388..>8036)
                     /locus_tag="ACG9CY_002383"
     CDS             complement(7388..>8036)
                     /locus_tag="ACG9CY_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFS4938866.1"
                     /translation="QVAESFEKKHQHVLRDIDALKKDVSNFGQMFVEGNEPDSYGRNR
                     RVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVPQTIEDMMIYQLE
                     EMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGKESRAYEEISKKVFSKFW
                     SEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQLNRQQRLFGDDNE"
BASE COUNT         2195 a         1656 c         1228 g         2957 t
ORIGIN      
        1 gagcaattgg aacaaattgc tggagttggt tatagaaaag ctctagaatt tctaattaaa
       61 gattatgcaa tatctaaaaa ttcagaggat gaagataaaa taaaaaaaat gttattaggt
      121 aaaacaattg aaaattattt gaatgatttt cctaaactcc aaagattagc caaagctgct
      181 acttggattg gtaatgatga aacgcactat gttcgtagac atactaataa agatgttcat
      241 gatatggatg catttattaa atcagcagcg caatttatcg cggctgatta tgatgctgat
      301 ttagctgatc aatttataaa tggaaataac tttatgccaa ctactaatta aaagaataaa
      361 agagccacag ataccaatta ataaaacggt tgggatatct tggctctttc atattttttt
      421 gacactaata gaatatcatg ctgctaacaa tgtttacata gctacaatga ccgaacattt
      481 agtgaacagt gtaattttag aataaagaga gttgttcttt gtacttctcg ctattgtaaa
      541 aatcaatttc gttttctttt ttcaatcggt cagcttgtct gtgttcatac tcatctaaaa
      601 agttcaatgt ctttctaatt tctgcatgtc gctgtctgac atacgatgag ctatacccaa
      661 cagattcagc aatatcttct aagctcattt gatcaacata tttcatctta acaatttgat
      721 tatcgatacc agaaaagcta tctatcagca ataacatttc ttctttttgc tcgatcaaca
      781 attccaattc attttctatc ttttggatat tctcttctaa agaagaagtt ctcgaattct
      841 tttcgatgcg gacgtttgct aaatcaccac agacccatcg attcaattca agcttacttt
      901 tattaagatt ccactttaag taaagaatct gctcatctag ttcttggtaa tcttttaacc
      961 aatgaaatct cacaaacgcc accccttatg gtagaatagt attgcgctgc tatctcgaaa
     1021 ggggtagctt ttttattttt tcttagccgt tccattctgg attgcctttt gttccaaacg
     1081 tcgttttttc ttcttaatct ttgattttgt ttttcccata caattctcct ttccgaaaac
     1141 acattttgtt cgatttttaa tactaaataa aagttggcca gaatttttcg ttcgtcttta
     1201 ctcatctttt tttgaataag attactagca taaaaatagt tagtaccacg aaaatgaaca
     1261 ggtaaatcaa ttacgttcac cttcttcatg ccagttatca gccactagaa tctttgcgtt
     1321 agtgccgcca ccgttttctt ttttgtattt ttcaaatgct ttataagcag ctgtttttga
     1381 tttatagatt tttccggaac cgactgggaa catccatgtg ccctctgtga tagctaagat
     1441 gtatactttc atttaattca cccctttaat ccccaacact acatatccgg acttctgcat
     1501 gtaatcagta atataagtga tctctgcttt gtatgagtca ccagtgtaac taaatgttgg
     1561 tctcgttagt ccaggtaatt catgtttaat gtatttttta tattcacgta gtattaactg
     1621 atcaccaact ttgaagtcac gatcattctt gcggatctcg aattgtttgc gtccgctcgt
     1681 cactgcttca aaatattctg gtagtatttt tagttcgtgg atcattctgt ttcctccttc
     1741 aaaattcgct catcatcaaa aagtgaatct ccacaaaatc cttctcccaa atatttatcg
     1801 ttcatgtata agtcaaccaa atatttgtga tctgcttctc gatattcatt aatattaacg
     1861 atctttattt tgtaaacatt accttcgtcg ctcactatct tgaattgttc tctgactttc
     1921 accaccgttc ctcctctttt tgaaaaaatt ttagtgtttt tgtaagaccc cctaaatcag
     1981 cttttttatt tgatataata cttgtatcga ttaatcaaat cgaaattaaa tttaaaaagg
     2041 tgatttatca tgaatatcaa caatttagta aaactcgttg aacaacgttt taaagaatca
     2101 ggattagaac aattaaaaaa agataattct ccagaattat tttctaattt taccgaagac
     2161 gacattccta ctcttaaaca attaaaatat tattctgatt attggacgca aactcgtgag
     2221 aatgaattaa atttagaaaa actcattatt aaaaagaaat agcaactcca gcccacacgg
     2281 ctggtttttt agtttcaact tccctcctta cgggtcaaaa tcatcggttc cggcataatc
     2341 tgatcatcct ttagcaacaa atcaggatat tttttcctta gcttcttatt gttcgtgtaa
     2401 gttttcaata gccattggtt ggcttcgctt ttgctttctc cagaaaaata aattttccct
     2461 gtttggatgc ctgcaagttg aatcatcatt ttcacctcaa atcaaatttt ttctgctaga
     2521 tggagcaaaa gcaatccgac atcaagaatc ttttgaccca ttatttcagc tactttgctc
     2581 ggcttaactc cttgagaaaa gagcaagcgt gcttgctcta tctcttcgtg agtccaaata
     2641 aatttggctt cctcaaggat aattactgga tcagttgctc tcattgttcc tctggaaaaa
     2701 tgatgtcatt cttatgcttg gaccaggtat ctgcatatgg aacaaagtat tgtctagcta
     2761 attcgatttg atcaattaaa gcagtttctg ataattcatg gtctgcagca atttcattca
     2821 tactatcgcc ttcatctaaa cagataagac cgttgacgtt atcgattttt acatttgtga
     2881 ctgcttgact ttctccacgc tcataatatt ctacagttga tactgataca tcagcttcgg
     2941 agtttaattg cagaatctgt tactgccggg ataatttaat attcttttca taattcacct
     3001 tttttcctac tgacatcgaa tatttttcat gttatgatta gccgtgaaag gagagctata
     3061 ttatgtgttt acacatgatg tccttagctc attcggttct ttggaagctt ttttggaaat
     3121 aactctcagg ttcttgacgc aaatctcatt tattctttga aacaaaaaac ttccattttc
     3181 cgagagcttt ggatcacttc aaaagagatt tgcatttata tttctgtgac cttgagtcgc
     3241 gtaaagaacc ttacagcaat ggcggcaatt ccactttcag gaaaccgcct tttttcattg
     3301 atattggtgg ttagcggact acccattttc tttcggcagt gccaaaccac aaatgatctg
     3361 tgttgatgtt taggtagttg tataccagat aagctaaatc caaagtgcct gcatcttcaa
     3421 gacgacaata ttccaaaaat tctcccatag ctggacataa aaatcctgga gctatcatat
     3481 cccaacagtt aatagtgcaa agaaattcgt ttagcttctg aacgttttct ttggtttcac
     3541 gaattacaac atactccaat tcttctccgc tggcagagca ttccaaaact tcaaattgat
     3601 taagaaatgg tagaacatat gcttcaaaca tcaacattcc atctttctgt ttttgcactt
     3661 gttttgcatc aatcatgtct atcctcctat tcagttattt cttccattaa tggaattatt
     3721 tcaggtcact ggacttaacg aaaacaccat tgaccatctt tccagtccga cctttgattt
     3781 cgtcataagc aaaatccaag cactcgtata aatccatatc attttgcatt gccaagatga
     3841 tcaaggttac tactacatcg ccaattccgt ctcttaaacc atgttcatct tttctagcta
     3901 gagaagcggc aacttccccg atttcttcga ccgtttttaa catttgcttg ctgaaatccg
     3961 cttgatccaa tcccttatct tttgcccact gctctacttt cgcgattagt tcgtccatta
     4021 tttctcctcc acatacctaa actgtcttcc ttttgaatca atccataagc tcctagctct
     4081 atcccaaata atatttttgc tcagaccagt aatttcagat aactgtttag cggtacccgt
     4141 tactagaatt cgatcaccat gccagattgc aatctttctc ggcgttctcc gtttgggctt
     4201 ttcagtccac attgatttac cgagcttttg gacttctgca actatttttt tatcttcctg
     4261 ccaatcttct gaataagtca attcgataat tcgctgcatt gccgctttct tatccatccc
     4321 gacattctcc tttcagtaat ttgagtactt gatcaagtgc gctctcacgt ccgccatgaa
     4381 atgtgttgag ccacttgtct tcgtacgaca cactttgtct taaagcttct tgatgcatta
     4441 attcgatctg tgctgtaaat gtctttaggt ccatcttgtt tacacctgct caagttcatt
     4501 aagatgtttt tgcaagcctt taacacaatc aatgaatagt aatttggtat atgctaaatt
     4561 tcttaattgc gttccatcga tatagagtgc gaaatagtat ctgagtttac tccaactcga
     4621 acgatcattc ttaattcctt caattccagc ttcttcgagt tgatcatata cgtctctcag
     4681 gacgtctaat tcctcgccct ttttataatt cgctatttca ttaattagtt ctagataatc
     4741 gatcttcatt tttccacctc ttagaatggt gcttttgatt gtctattagc tcgttctagc
     4801 gcttttttct tgagataggc ttcttggtcg attgcccatt caggaagctt ctctcgtctt
     4861 cctgtgcgct tgtatccact gcttgcgttc ttaggttcac ttttttcttt ccttgcccaa
     4921 cttcgaatag ttgccaaata gtttttataa gtcttaccag atgattcaca atactctgac
     4981 agtcgttcga ttcgctcttg gtaatcatta gggaattctg ttttgagttt ctccatctgc
     5041 tcatctgaca aaagaacatt tttatactct ccgtatttat gacggacggg cttagccttc
     5101 gattttttcg aaggcggtaa ctctcttata tattcttttg tattattaaa tgtattatta
     5161 atagatgtat tattatcttt gactttttcg tcaatagggg tattgcgttt ttcgtcaata
     5221 gggtattgat taattcgtag gtacctattg attatttgat tggtaccctc tttgtaaatg
     5281 atttcccgat tcaagtatcc aaacttaatc aaatcactta cccatcgcga tatggtctct
     5341 ttattcacac catataaatc tgcaaagtac tcattgcctg cccaacaaaa gcctctttca
     5401 ttacacaagg ccgttatctc tccgtataac aacttagtat ttggtttaag tcttttgtcg
     5461 taccttacgt tggctggtat aatcgcataa taacttcgat gttctgtcat ttttcccctc
     5521 caatatttaa ctttttgatt gttttctggt ttaatttgat ccctttgatt tgatatttat
     5581 ttttgaaatt aatcacacct attttgtgct tctctgtgtg atggattctg cagagtgctg
     5641 caaatgtgta ctctgcatga tcaacttctt tgcgctttcg tcttcctagc gctttgtcaa
     5701 agtgatcgat gtcagctcct gttttgccac agatgcagca gactcttttt gtgatgcatt
     5761 tgtagaagta atattcttga ttcgcaggta aaatctcata gccttctttg aaaggaatat
     5821 gatgttcaaa gatgaaatct aagatgatat ttgctaagac attggcatca ctcacagttg
     5881 tattcgattc gtctttcagg cttattttgc gccctgtgac accttcaaaa cgaaagtaga
     5941 agaattcctt ccagaagtcc gttggcatgc ctgtatcgat aaaaatatcg cctatcagcg
     6001 catagatgaa gtttcgttgc tgtacggtga aacgtctagg atcaataaaa cgaatttcaa
     6061 tgactcgatc accatcgtag ccgtcataca tcgtctttag tcgatcaatg ttcacttcct
     6121 cattgatggt tgcgcttatg tcttttcctt tgaacttttt cagaaccgct gaatatgaat
     6181 cgattaatgg tttaaacact catatcactt cttatctaat tcttttctct tagctgctat
     6241 tgctcgctcc atcaaggcac attgctcata gcttaactgt tcaatagttt caacgttatc
     6301 agctaagagc cctaatttat ctgtctgctc attaacatat tcgattaagg ttttggtcat
     6361 atctttaccc atctgctcat tgaaagcttc tagaatcgtc tctagcatgt ttaatttctt
     6421 tgtatcgatt ctaggtggtg ttggaatatc ttccccttga aatacatata atcccagtcc
     6481 gtgtagagcc aatgctttca caaagcatcg cttcaatgag ttattgattt gcattgcatt
     6541 tggtttaaca actggttggt ttcgataatc taaaacagga aataattcgg tttccgtgtg
     6601 tcctttaacc gttactgaga cagatacata agtcccagtt tcatccataa gaaaaggttt
     6661 atattcctca acaagaaagt cttgatgagt tccagaaaca accctgtagt gtttgtactc
     6721 attaatagtt accgttgcct gtggatcatt ctttttcata atctcccacg cgtgagccca
     6781 agataaataa tcaaaatttc cttttttctt gagaatttta tttaacttac gactaaaaag
     6841 tttttcaaag ttcgttgtcc ctttgatttc actcatcaaa ttctgcctcc atttcagcaa
     6901 tgtatttctt acctggtccg taataagaga tatcaatcaa gttatctctg tcgtactctt
     6961 ctagcgcatc aatcaagcca tcttcgatga cgtaaatata ttcaggttta ttcgaatgct
     7021 tcgatagatg gataagataa acatgatccc aaatagttac atggttgcct aaatcatctt
     7081 gatcccaagc tagttcttca ttcgtcaaaa gatttcgtct gatttttcga ccacttgttt
     7141 cctcaatttt cggcttgccc caatcaggat caatcaaata ttgatctaga gtggaaagtt
     7201 ctttttccat atgctaaaat ctccttatga tgtgttttct ttgtgactct ttgcttgccg
     7261 gcggagtcac ttttttattt gttgccatgc tttttgcttg tcaatatgtt gttggcttag
     7321 gatgattggt ttatagtatt tccaccagca attagcaatt gccgtcccta ttcttagcgc
     7381 ttcagctcta ttcattgtca tcaccaaaaa gtctctgttg tctgttcagt tgatcaattt
     7441 ccatacggat cgcagtttct ggcaaccaca tttcaataaa tgaaacagca tcatcgaatc
     7501 tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat tcagaccaaa
     7561 attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt ccccctagaa
     7621 ctcccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt ccgctaattc
     7681 gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct tctaattgat
     7741 agatcatcat atcttcaatt gtttgaggaa cagtattctt gcgaataaca tcttccattt
     7801 cgttgaatgc ttcaatgtat ttttgtttga agtagatagc tttctttcct gtaaacccca
     7861 tagccagcaa gaaaaatcca tctctactaa tgaagaaaac tcgtcgattt ctgccatatg
     7921 aatctggttc attaccttct acaaacatct gtccaaaatt ggacacatct ttttttagtg
     7981 catcaatatc tcttaaaaca tgttgatgtt ttttctcgaa gctttctgcc acttgt
//
LOCUS       DAZULG010000116         8002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000116 DAZULG010000000
VERSION     DAZULG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.120"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1448
                     /locus_tag="ACG9CY_002384"
     CDS             <1..1448
                     /locus_tag="ACG9CY_002384"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFS4938867.1"
                     /translation="FLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAETAEA
                     VKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGEVQR
                     GIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWIFPM
                     ALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPLVKG
                     ISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFGSAG
                     ERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTFDYI
                     QKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVKDLM
                     AAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGWKHS
                     FFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1524..2684
                     /locus_tag="ACG9CY_002385"
     CDS             1524..2684
                     /locus_tag="ACG9CY_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFS4938868.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2797..3804
                     /locus_tag="ACG9CY_002386"
     CDS             2797..3804
                     /locus_tag="ACG9CY_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4938869.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3828..4853
                     /gene="iolG"
                     /locus_tag="ACG9CY_002387"
     CDS             3828..4853
                     /gene="iolG"
                     /locus_tag="ACG9CY_002387"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFS4938870.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4870..5760
                     /gene="iolE"
                     /locus_tag="ACG9CY_002388"
     CDS             4870..5760
                     /gene="iolE"
                     /locus_tag="ACG9CY_002388"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFS4938871.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5783..7378
                     /locus_tag="ACG9CY_002389"
     CDS             5783..7378
                     /locus_tag="ACG9CY_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFS4938872.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7394..7708
                     /locus_tag="ACG9CY_002390"
     CDS             7394..7708
                     /locus_tag="ACG9CY_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4938873.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
BASE COUNT         2367 a         1520 c         1932 g         2183 t
ORIGIN      
        1 gatttttaag aaattatatt gatggtcgtt gggtcgctag tcgctcaaca gagtttgaag
       61 aagtaatgaa tccggctaca gaagaagcgc tggcgaatgt accgttgtct agcaaagcag
      121 agacagccga agcagttaaa gctgccgatg ctgcgtttca aacttggaaa aaagttcctg
      181 ttcctgttcg cggacgttat ctgttcaagc tgcatcagct tttaacagat cataaaagtg
      241 aattggcgcg tttgatcacg ctggaaaatg ggaaatcatt gtccgagtca ctgggagaag
      301 ttcaacgagg aatcgaaaat gtggagcacg cagcgggaat caccaattta atgatgggag
      361 attctttatc aacagcggcg actgatattg agatcacgaa ttatcgttac ccaatcggag
      421 taatcggagg gatcacaccg tttaattttc caatgatggt gcccttctgg atcttcccga
      481 tggctctggc ggcaggaaat acggtggtgc tgaagccttc agaaaaaaca ccattgttga
      541 tggagaaaat tgttgagctg gttgaaaaag caggctttcc tagcggtgtt tttaatgttg
      601 tctatggtgc ccacgatgtt gtcaacacat tgctgcaaga tccactggta aaaggcatca
      661 gctttgtagg atcaaaaaat gtcggtgaat acgtttaccg tgaaggaaca aaaaatctaa
      721 aacgggtaca agcgttgact ggagcgaaaa atcatgttgt tgtgctgaaa gatgccaata
      781 ttgatcgggc agtcaaggac cttattggcg gagcttttgg ttctgctggt gaacgctgca
      841 tggcagcctc cgtcattgtt ttggaagagg aaatcgcgga tcagtttgtt gaaaaattta
      901 aggctgcggc aaaagcgatc aagatcggca atggcttgga cgaaggggtc tttttaggac
      961 cagtgattcg aaaagatgca cagcagcgga cctttgacta tatccaaaaa ggggtcgatg
     1021 aaggagcgaa actggtacta gatggtcgcg aaaacattcc agaaaaaggc tattttgttg
     1081 gaccaacgat ttttgataat gtcacgatgg atatgaagat ctggcaggat gagatttttg
     1141 ccccggtcgc atcaatcgtg cgggtaaaag atttgatggc agccgttgag ctgacgaatg
     1201 aatctgaatt tgcgaatggt tcgtgtctgt ataccaacaa cgcatcagcg attcgctact
     1261 tcagagaaaa tgttgatgct gggatgctgg gggtcaatct tggggtgcct gcaccgatgg
     1321 cttggtttgc cttttcaggc tggaagcact ctttctttgg tgatttgcat gccaatggca
     1381 aagacagtgt tgagttttat acccatcgga aagttgttac agcacgttat gcgcaaagtg
     1441 agatttaaag atttgcttcg aagctctgtt aaagagcttc gaggattctg ttataggcta
     1501 taagccagag agaggttttt tctgtgaata ttcaactgaa aatgcctgcc aatttaagaa
     1561 ttggcggcgg aacaatccaa tttcttatgg aagaatcaga acaactaggc aacaagccgt
     1621 taatcattag tgatacggtt atggaacaga ccgggcatct tcaaaagatc aaggagctgt
     1681 tgggaaaaac agatgatcaa ttagcggagt atacagacat tcaagccgaa ccgacgcaag
     1741 aaaatgttta tgatgcgctg aagctttttc agaagcatca atgtgatcat atcattgctt
     1801 ttggtggcgg cagctgtctg gatacagcca aggtggtagc cgttttggct gctaatcaat
     1861 taagtctcga agcgattcat gcaagaaatt ttgatcagct gcaagcgggc attccgctga
     1921 ttgcagtgtc aacaactgct ggaactggtt cggaagtaac cgatgtggca gttattacca
     1981 atacaaaaac agcagtcaaa atgatgatga aggatctcgt tttcatgcca cagatctcaa
     2041 tcgttgactc tgattttacg aaaactgtac caccaaaagt aaccgcggca acgggagtcg
     2101 atgcgctatg tcacgctttg gaatcttatg tttcagtcaa agccaatgaa gtaacgcaac
     2161 tattttcact ggaagcgatg aagaaaattt tgaacacgct ttctcaggca tatgaaaata
     2221 gcgacgacac tgaagccaga gaacaaatga gcttagcttc aacggaggcg gggattgcct
     2281 tttcgaatgc gtcagtcaca ttgattcatg gaatgagtcg accggtgggg gcactttttc
     2341 acattcctca tgggatatca aatgcgatgt tattgccggt cttttttgaa ttctcaaagg
     2401 atgctattca gactgagtta gcgacgatcg ccagatttat ttatccggca gccgaagatt
     2461 tatcgctgga agaggctgcg gattatctga ttaaaaaaat ccaagagctc tgtgagcagc
     2521 tgtcgatccc aacgattcga gattatggta ttgatcagca agcgtttatg gcagccttag
     2581 acaaaatggc cgcggatgcg ttgaccagcg gaagtccgca aaataataaa aaggtgccta
     2641 gtcatagtca aattaaggaa ttatacgtac aagcttatgg gtagtaaaag gcggagcaaa
     2701 gagagggctc taccttttaa gaataaaagc tacacaggaa taattttgta agcgcatact
     2761 atgtgtgctt aaacaacaga acagggggat aagtaattga gagtaaaagt aggcgttatc
     2821 ggaacaggtg caatcggcat cgagcatatc gacaggatca atcagaaaac acaaggggct
     2881 tatgtgacag cagtctcaga tatcaatgta aaagctgcta aagaggttgc caaacgtgtc
     2941 gatgctaaat tctttgaaac cggagaagca ttgatcgctt ctgaagatgt ggatgtcgtt
     3001 gttgtgactt cttgggaccc gacccatgag cagtatgtgt tagaagcaat caagcatggg
     3061 aaatatgttt tctgcgaaaa gccattagcg atggatgcag caggctgccg ccggatcgtt
     3121 gatgcggaag ttgcttatgg gaagaaattg gttcaggttg gcttcatgcg ccgctatgac
     3181 cgcggctaca ttgaactgaa agaagcaatc gaatcgaagc aatacggaga agcattaatg
     3241 atgcattgtg cccatagaaa tcctagcgca gatgaaaact acacaacacc gatggcgatc
     3301 agcaacaccg cgattcatga gattgatgtg cttcgctggt tgctaaatga agattatgaa
     3361 tctgttcaaa tgatcttgcc gaaaaaaacc agcaactccc atgccgattt gcatgatccg
     3421 cagctgctga tctttcaaac aaaatccggt gtaacgattg atttggaaat ctttgtgaat
     3481 tgccgctttg gctacgatat taaatgtgat gtggttttcg aaacggcaga agtaggaatg
     3541 acggacccag cctatacacg aattaaatcg gaagaaaaaa attacacacc tgtttcacca
     3601 gattggcaga cccgcttttt ggatgcctat gattatgaat tcaagctgtg gatcgattcg
     3661 atcaaaaatg atgcggttgt cggtcctacc gcttgggatg ggtatgtagc agcgatcacc
     3721 atgatggctt gtcatgagtc tcgtgaaaca aaaacaaaag taatgattca aatggatgaa
     3781 cgaccagcac tatacaatga gtaattctca gaaaacggag gtatttcatg aacaacatta
     3841 gagtaggcat cgtcggttta ggaagattag gaaaagtaca tgcacaaaat ttagcaacag
     3901 gtgtcagttg ttgtgagctt acggctgcgt gcagtttagt tgaaacagaa ttagcattcg
     3961 cacagaagaa attaggcgtc aagcatactt tttccagcta tgaagaaatg gtcaaaagtt
     4021 ccgtgatcga tgcagtcttt attgtttcac catcaggctt tcactgtgac caagtccgct
     4081 tggcgatgga aaatggcaag catgtcttta ctgaaaaacc attaggcctt gaagttgaag
     4141 atattaaaaa gacccaaaaa gtcatcgaag agcatcctga acaggtcttc atgttaggct
     4201 tcatgcgccg ctatgatgag tcgtatcagt atgcgaagaa aatggtagaa gatggcgaaa
     4261 tcggcgaaat cacactgatt cgttgttatg ggatcgatcc gagcgaaggc ttggaaagct
     4321 ttgtcaaatt tgccatgaat aacgacagcg gcggcttgtt cttagatatg gcgatccatg
     4381 atattgattt ggttcgctgg tttaccggcc aagaagtcga tcaagtgtgg gcgattggga
     4441 aaaatgcggc gtatccagaa ttggatcagg ctggtgagct tgaaactgga gccgcaatga
     4501 tgagcctgag cgataaaaca atagctttac tagttgccgg cagaaacgca gcccatggct
     4561 atcatgtcga aacagagatc atgggaacca aaggaatgct gcgggtagcg caagcgcctg
     4621 agaaaaatct tgtgacgatc atgaacgatc aagggatcat ccgtccatgt tcacaaaact
     4681 tcccagaacg attcagagaa gccttcattc gtgaagccca agaatttgtc tcatgtattc
     4741 aagaaaaacg tcaaccagcg gttaatgcag aagatggatt gcaatcaact aagatcgcgt
     4801 tggcttgtca agaatcattc gatacgaaga gtttagtaca actaaaaaaa taagcaggag
     4861 tgtgagaata tgaccaaaca aattcaatta gccatcgcac caatcgcgtg gaccaatgat
     4921 gatatgcccg agcttggtag tgaaaatacc tttgaacaat gtatcagtga aatggccctt
     4981 gctgggttca ccggaacaga gattggcaac aaatatccaa aagatccaga ggtgttggaa
     5041 caatatttga atattcgcgg cttaaaggtt gccagtgcat ggttcagtgc gtttctgaca
     5101 actaaacctt ttgaagaaac tgccgaagcc ttcaaagcac atcgggattt cttacatgcg
     5161 atgggcgcta aagtcatcgt agtagctgag cagggtcata gtatccaagg gattatgaca
     5221 gcgccagtct ttgatgataa accagttttc actgatgaag agtggaaaaa actggcggaa
     5281 ggattagaaa aattagggga tttggcccac gaaaaggata tggagatcgt ttaccatcat
     5341 cacatgggta ccggcgttca aacgacggaa gaaatcgatc gtttaatgga aatgactgat
     5401 ccagacaaag ttaaattatt gttcgatacg ggacatttgg tcttttctgg tgaaaatcca
     5461 atcgcggttt acaaccgtca tcaagatcgt atcaaacaca tccatttcaa agatattcgt
     5521 caagaaacgg ctgaagaagt agaaaaggaa aaagacagct tcttaaacgg cgtgaagaaa
     5581 ggcgtcttca cggttcctgg tgatgggatg attgatttca ctccaatctg gacagcaatc
     5641 catgccagcg actatgaagg ctggatcgta gttgaagcag aacaggaccc ggctaaagcc
     5701 aatccatttg aatatgcagt caaagccaga agctacattc gagaggtcac cggtctttaa
     5761 tctattgaaa aggagctaga cgatgaatct tttttctata atcagtttcc tagtgattgt
     5821 catctgtgta tggttatttg cctattctag atccagaagt gtcgatactt ctggatcaga
     5881 agggttcttc atgggagggc ggagcttaac tgctttgcca atcgccggca cgatcatcat
     5941 gacgaacctg tccaccgaac aaatagtcgg acaaaatggt caaagttatc atgcagggat
     6001 ggaagtaatg gcctgggaag taactgctac gattgcgatc gtagctttgg ctgtgatatt
     6061 cttgccaaaa tattttaaat acggcatcaa tacaatctca gatttcatcg agattcgtta
     6121 tgatacggtt accaaacgaa ttatttcgat cttatttatt gtgacctata tgacttcttt
     6181 cttacccgtg gttctttatt ctggatcgct ggtgttcaat aagatcttcc atattgatga
     6241 gctgctgggc gtccagccga tcgtcgcgat cataattgta gcgatggcaa ttggtattgt
     6301 cggcatcctc tatctgctga tcggcggctt gtcgttaagt gccaatagtg actccattta
     6361 tggagtcggt ctgctggctt gtggtctgct ggtcccaatt ttaggcttaa tgaagcttgg
     6421 tgacggcagc tttgttggcg ggatcgaaca cgttgtggta aacacgccat ggctgcttaa
     6481 ctctgtggga tcgatcgact cggctgttgt tccatggccg acactattta cagggatgct
     6541 gtttaacaat ctgtatttct ggtgtaccaa tcaaatgatc gtccaaaaag cattgtccgg
     6601 caagaattta gctgaggcac aaaagggcgc tttcctagtc ggcgtcttca aaatcttagg
     6661 tgcgttattc cttgtctttc cagggatcgt cgctcgcaat atttttggcg atagtttaat
     6721 gtcaaatcca gataacgcgt atcctttttt agtcagtgaa gtattgccta aaatgatctt
     6781 tggtattttc gccgctgtta tctttggcgc gatcttatca tcatttgcgg gagctttgaa
     6841 cgcgacatcg accttgtttt cactggattt ttataaaccg cttatcaata agaaagcaaa
     6901 tgataagcaa atcgctcgtg ctggtaaaac agtaacggtc gttgtgggga ttatctccat
     6961 tatcgtagcg ccattcattt cttttgcacc ggctggattg tatcagttcg ttcaagaatt
     7021 caatggacta tacaacatgc cgctgctcgt gatcattctc tttgcctttt attcaaaaaa
     7081 agcaactgct tttggggcaa aaacgacgat tgccgtgcat atcgtcttgt atgcactatc
     7141 aaaaatgctt ttccaaaata tccatttcct ttatgttttg acggcattat tcgttctgga
     7201 cattatcatt ttgatggtgg tttccaaatt gaaacctgat ggagaatttg tgttgaactc
     7261 attcgataca aaggtcgata tcgagccttg gaaatacacc aaggtgactg ctatagcatt
     7321 ggtagtcatt gttattttaa cttatgtggc attttcacca attggctttg caggctaaaa
     7381 acaggagggt attatgcata aggatctatt gattatttgc gagaccggaa tcagtgcgtc
     7441 gctgctcgta tcaaaaatgc ttgaaacgat cagagccaaa aatctatcgt atgagataga
     7501 ttatgcaacg atccgaaacg ttgaagaaaa actgaattat aaagactatg acgttttact
     7561 tttaacgccg caggtcgcac gttctgagga aaaagtaaaa gaaatcatca aaaaggaaaa
     7621 atgtaccgct cagatcgttt atatcaaaca agaagatttt cgttatatga atattgaaaa
     7681 catcattgca agcatcgaag aagattaacg agaatctttg attattgagt gtcgaggagg
     7741 tggtatatga aaagatggca tgttcgcccc gatcacccca atatgaaggg cggacttatt
     7801 agaaataccc aatcttgtat ttattcaata gggtaagcgg acctcaatag ccagctcatc
     7861 cccaaaaagt cgaatggaga cacttaaatg agtgtaccta ttcgactttt tgagactcta
     7921 agtcaaatcg tgttgttctc attaaatcac tgaaaggaaa tgcttcggcg tttccttttt
     7981 tctttttgtt tttcttcgtg tt
//
LOCUS       DAZULG010000117         7932 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000117 DAZULG010000000
VERSION     DAZULG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.121"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2671)
                     /locus_tag="ACG9CY_002391"
     CDS             complement(<1..2671)
                     /locus_tag="ACG9CY_002391"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4938874.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            3239..4459
                     /gene="sstT"
                     /locus_tag="ACG9CY_002392"
     CDS             3239..4459
                     /gene="sstT"
                     /locus_tag="ACG9CY_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS4938875.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4606..5241
                     /locus_tag="ACG9CY_002393"
     CDS             4606..5241
                     /locus_tag="ACG9CY_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4938876.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5432..6172
                     /gene="truA"
                     /locus_tag="ACG9CY_002394"
     CDS             5432..6172
                     /gene="truA"
                     /locus_tag="ACG9CY_002394"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4938877.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6317..7930)
                     /locus_tag="ACG9CY_002395"
     CDS             complement(6317..7930)
                     /locus_tag="ACG9CY_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938878.1"
                     /translation="MDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLSRIIGLDNV
                     WSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVS
                     YFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYI
                     RDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVL
                     TFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLG
                     LIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSL
                     FIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAV
                     AIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNT
                     VLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYL
                     SIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2625 a         1234 c         1489 g         2584 t
ORIGIN      
        1 ttttttgtgt cgcccatgtt gcactgtact ggttacctgc aacaagatca tattgtatca
       61 gacgataatc ttctagaatt tctttttgtt tctcagttaa atttttttct tggataattt
      121 gactatcctc tgtaacaaag acctgttttc cattaaaacg attagacaag ctttctgtag
      181 gatcaattgt taatgcaggt agatgattag atacttctgt caacaacgca tagtaaggag
      241 ttactttgat attcgcatgc tcaagagcca tagcaccaaa gttattagga gcaacaactt
      301 ctttatttaa cgtagctgct tgttcacgac tatacttgtt gttataaatg aaataatctg
      361 tctggtgctg ttctaatcca tattttttcg aatcattccc actatagatt gcgggtaaat
      421 ggtcaccata aaaaacaaag gtaatcggtt tatcgatttt gtcgatttct tggatgaatt
      481 tttttactgc tgtatcggta tagtgaaggc cttgcatata agtttgtaat tcttttcttc
      541 tattaggaat gacagcagtt ccttcagcag tgtagttcaa ttcactatag taatcatcat
      601 aaggcatatg gttttgcata gtggataatt ggatgaattg tgacgtttct gtattgcttt
      661 gtaaagcttt caaggtttct ttgtatgctg aatcatccga tatgcgtggg ctttgttcta
      721 ttttatccgt ataagttaat ttatccggac tgtctaaata atagaacctg tcaaaaccaa
      781 atttatcaaa cacttgtttt ctgcgataaa gagcagctgt aaaaggatgg atggctattt
      841 tttcatcgaa tagatcggta atatttggac ttattttctg acgctccact agctgggtat
      901 aaggtgtaac taacgaagca gacaggttac tgatactcaa gcctgtcagt ccttcccatt
      961 ccatgttagc tgttccgcca ccataaccga cggataacat caatcctcct gttccttctg
     1021 ttataatgct tttgatgtaa ggaatagggt ctgtaggtac tgtcagatta gggacacgtg
     1081 caggatcact gaagctttca cttaaattga gaacgatcgt ctgattgttt aaccagtcac
     1141 ttcttgtttg gttgatttca tctgcctttg tgttgtactt tttcattatt ccattaattt
     1201 tttctttcga atagccagca ggttcttcca tgattttaat gtcgatattg tttaggaatt
     1261 gaatgactgg tccgttatct tttacggcgc cttgctggtt aaagaaagtc ttgtttactt
     1321 taaataaatt aaacagcaag aaagaaggcg aatttttgtg attaatgaaa aagacacccg
     1381 aaaaacatcc taataataca gcgatactga taaatcgacg tttccaatga atcttcaagg
     1441 aatactgttt ttgtagtcta cgttgtacaa taatactagt aatcaataat aggagaagga
     1501 tcaatccgcc cacgattaat aagactgggc taaccatcga taatagttct gacaatccgg
     1561 tcagtatttt taaatctgaa ggaaagacag gctcttcccg catttccatt ttgattactg
     1621 tcgaaatagt gagcagtaag ttaatgatca aggtagcagt caaggcgtac caaaaacgat
     1681 tggaaatcaa caatagtaag aagaaaaatc ccataataat gatgattgtc aggatcatgg
     1741 gtgcttgtcg ctgagtaaat atttttaagg caacctggat agtttctttc caaccttctt
     1801 ttggttctaa aacgaaaatc tgcacgaagg taaagcagta aaaaaatgct gcaactaaaa
     1861 aataactttt atgaacaaaa agccaatgaa ttcttttttt ccaacgctga gttaattgtt
     1921 caatcgaatc aaaagcgaag tattcgatga aacgatgaat gaaacggatt ttttgtatat
     1981 aaaataaaaa gataactagg agggacactg ctaataaata gcaaataaaa aactgcccga
     2041 tatttatcag ccatgccttc cctgagtttg gaaattcacg tttcataaat gtagatacgc
     2101 gataagaaac taaagggaaa ttacatataa cttgagtgac aatcaaaaat acagcaagaa
     2161 aatttcctct tccttttata cctaatttat cagatagcat ttctaaaaat aaaaacgaag
     2221 agaaagtata atacattgcg aagagagaat atggtttaga aaacctcagc gctgtatctg
     2281 tatttcctct gatgatgagt gaaatttgtg tcatcaaaat aatcaaaccg aataaaacaa
     2341 tcaagctgat aaatacttga ggaagcttat agcggacctt ttctttccag ttccattctt
     2401 tcaaaccata acccattaaa aataaataaa aaatccataa aatactctgt ccattctgta
     2461 atgcaaacgg gtctttatta aatagagtag agcagccaac taatccaatt gttaaaaata
     2521 atagtagata tttaattgtt gttttcgata atttatttaa aaatccaact gtataaggaa
     2581 tactacaaaa aagcaaagta caagaaacag cataagtaaa gtaattctga ctgataggtg
     2641 ttaggataag ccaataatca cgaatattca agctgccagc ttgtacgtaa aaaaaagata
     2701 ttgcaaacta gtgtggctat taggaatata aaggcaaaac gacttaaata atgtaaaact
     2761 tttaaacggg cgattttttg atattggtaa cccatgtaaa gtgggatcaa atttgcacca
     2821 ataacaacgg aagtttgtaa aaatgtatag atgtagaatc gaccagggtt tgttgcatat
     2881 gtaggaatag tgactgctga aatcaatata cgctgtaaaa ccatcattat acaagcgaca
     2941 aagattgtca ggaagccata actctccttc tttatttttt tcaagaataa acacctcaca
     3001 aaaaaattgg acaaaaacat aagaaaacgt ttcctgataa gttgcccatt aataacatcc
     3061 taacttagaa tatctcaaaa aacaacgtgt agacagaaaa agtacaaaat tagcaagaaa
     3121 tggttgtttg tattgttaga atttttagaa aattatacct ttagtttctt tgcataaaat
     3181 gatacaatga ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt ttttggatat
     3241 gtcgttagtt caacgtattg ttgttggtat tttagtcgga atattgtttg gtatcttttt
     3301 ccccacatgg acatttattt caatattggg aacgttgttt gtcagttgct tgaaggcaat
     3361 cgcacctatt ctggtttttt tcttgacgat ggcgtcaatt gccaagcata aaattgggaa
     3421 taagacattc gtgaagccta ttttgatctt atatttagtg ggaacattgc tttctgcact
     3481 tgtagcagtt attgtaagtt caatttttac tgttcctatt actttacaag aaacagtatc
     3541 tgaaaaagca ccgcaaagtt tggaaagtgt tttatctact atgttgacga atgtcacaca
     3601 aaatcctgtt caatctctta ttgatgcgaa ttaccttggc gtcttattct gggctgtatt
     3661 gttgggacta gcttttcgag cacgttcaga atcgacaaaa gagttggtgg atcagatatc
     3721 tatagcgttg tcacaagtcg tacaagtgat tattgcattt gctccgatag ggattttagg
     3781 attggtttat caatcaattg ctacgactgg aatagctgga ttggcagagt atctgcagct
     3841 tttacttgtt ttgatcggta cgatgctgtt tgtcgcatta gttgtgtacc cattcctcac
     3901 gtttttgttt attaaagaaa atccctatcc attgattttc ttttgtttga aagaaagcgc
     3961 acttcccgct ttctttaccc gaagttctgc cgcaaatatc ccgataaaca tgatgttggc
     4021 tgaaagattg aaattgacaa aagagtctta ttccatatcg atcccgttag gtgctacgat
     4081 caatatgggc ggtgccgcga ttaccatttc cttgatgacg ttgactgctg tacattcttt
     4141 agggattgaa gcaccttttg ttttgaaagt ccttttatgt attttgtctg ctttagctgc
     4201 ttgtggagct tcaggtgttg cgggcggttc actgttattg attccattag catgtagttt
     4261 gtttggtatc tctaatgatg tcgcgatgca aatcgtcggt attggattta ttatcggtgt
     4321 tatccaggat tcaatggaaa ctgccttaaa ttcgtcaagt gatctgttgt ttactgcaat
     4381 aggagagcta ggcgcgcgta aaagaagtgg ggaaaaaatc aaattgaaag attgtttaga
     4441 tggattaagt gaacgttgaa cgttagatat ttagtatttt taaaatgatt cgagttttta
     4501 atgagttata tagatgttcg tgtaaataga ggtttctgtt aaatcaagat aaagtcgctt
     4561 ataataacaa ttaaaagcat aggtaattgt tattggaggc gtatcatgga ttttttacgt
     4621 aaatttggtt ttttcttttt attattaggc gtgctgagtg attttttgac tccgtacgta
     4681 ttaggattgt attatcctga attagatcaa atgaagagcg tgatcagtgt atttggagag
     4741 gtttccagtc ctgtaagaaa agcgtttctt atttggtctg tggtttcagg gatctttttc
     4801 atcctgtcat tgcctgcatt gtatgctgca tttgtaagta catctcgtgt gttagctacc
     4861 ctagttacag cagccatcgc gttctatggt ataggtgatt gtatctttac aggtttgttc
     4921 agtatcaata cccatgaatc ttcgtggaat gtctcgacat gggtccataa tatcggatca
     4981 ggtttaggtt acagtggttt tttattgttt ccagttcttc ttttcttgat ctatcgaaaa
     5041 aatgggcaaa tggacgtaag ccacttatac cttggactgt ctgtgatcag tttgcttttt
     5101 gcggcgattt atggactggc aagaattcct tctataaatt cttggccatt actgaataaa
     5161 ataggttttt gccaaagaat cagttttttc tttaattatg tgcccattgc ctggttgagt
     5221 atcttgcatc tcaaacagta aaacttgagc cgtcaaattg aacagtcagc ggatacttca
     5281 tccggtggct gttttttgtt tttctgaaat atatatttcc acagttttct tgtatagctt
     5341 ctgttttaga gaagaaagga gctatatttt gtgctacaat cttaaaaagg tagagagaag
     5401 accaaatgga agacagagga gccactaaaa tatgagaaac atcaagttga caatcgaata
     5461 tgacggaaaa agatattcag gatggcaaag acttggagat gacgataaaa ccattcaagg
     5521 aaaaatcgaa aaagtattgc atcagatgac aaatgaagag atagaaatta ttggatcagg
     5581 aagaacagat gctggcaccc atgcgcgagg acaagtagca aattttaaaa caaacacaga
     5641 aattggactg gcagaaatga tcgactttat gaatcgttac cttccgcggg atattgtcat
     5701 caaaagaatc gaagaaatgc ctgagcgctt ccattcaaga tataatgcgg tgggcaaaaa
     5761 atacagctat tatgtttgga acaacgtgat tccttcagca tttgagcgaa accacagctt
     5821 ttatttccct caagagttgg atatggacaa gctgaatgca gcttgcgaaa aattagtggg
     5881 tacacatgat ttcattggtt tttctgcatt gaaaaaatca aaaaaatcca cagtgcgtac
     5941 gattgatgaa ttatctatcg aacgaaaggg agaaatgctt cattttactt ttgtcggaga
     6001 cggattctta tataaaatgg tgcggattat catggggaca ttactagaaa tcggcacagg
     6061 tgccatgccg atagaaacaa tcgatgaaat atttgaaagc aaagtgcggc aacatgcagg
     6121 agaaaccgtc cctgcacaag gtctgttttt ggatgaagtt tattatcgat aaaaatactt
     6181 tgataatgga tgaaacgcat gtttcacagt ataaatatcg atgttaaata atgtatgaag
     6241 caaattaaag aaatataaaa aaagactttt tagcctgttt atgtgtacag ctaaaaagtc
     6301 tttcttaaat aaaaatctag tgagaatgtt gtacttctct tctcggttgt ttttgtttag
     6361 aaaaagtaaa tcgataaagt aaaaataaaa tcaaagtaac gatagataaa tatcttgaag
     6421 caattgctag ttttgtgtac tgataagtaa taacgacttt atttatacct ggaggaatag
     6481 ttaattcagt tgtgccaaac ttagataatt ttgtttgtac tagtttgcca ttaatagaag
     6541 ccacttgccc tttataacga taaataggtg tcgtcaagac agtattttta ttattcgaat
     6601 tatctaattc aattgtaagt ttgttactca taaaatgagt tgttggattt atttcctggt
     6661 tacctagtaa atattgatca ttattaatca tatcaggatg atagattgac tctttgtttg
     6721 cataatcagt atgattgtaa ttttcagcta tagcaacagc gtcttcaggt gctataagtg
     6781 ttaatgaatc tttgttagca tggatagaat tgcttactcc tgagagatgc aatattagtg
     6841 caagaaacgt aaagaccatt attttccatg aattattttt agtagaaaaa atataataga
     6901 tcactatact catcgtatag gctataaata aagaagaaaa accatttaac cgccagacaa
     6961 attgtagact cttaactggt gtatggttaa aaagttgcca aggaaataaa ttggttgaac
     7021 aaaaaagaac aaatacacca agaaagaaaa taaataaatc atcagcagtt aattttttta
     7081 taaaaataaa agcaccaatc aatccgagaa ataagaataa tccaatagta tagttgttat
     7141 agttattatt taaaatgtgt gtcagcatat cactaggagc cataccactt aattcttttc
     7201 ccagtgggac tttcaattct tgtgctctag attgttgaat aaaaggaatt aagaatcccg
     7261 cacttaaaaa aaaggtgagg acggtagctt ttaaaagact taagagtcta gcgatttttt
     7321 ggtcccaaaa ataaaaagaa agaaacaacg ttcctccaat aaatacagaa accatagcga
     7381 cagaaagtaa atgagtatac actactagcg tcatgccaat agacaaccag taccattttt
     7441 ggtaatctct aatataaatt tcgtaacacc ccattagtat caaaggcaaa aatgttaaag
     7501 cgacagcttc tcccaatgaa gcacgatgga atatgtcaat ggcacgatag gcggaaaatg
     7561 tataaataat cgaaaataac agcgaaatat aacgattgtt ttttatttgt ttcatagaaa
     7621 aataagaaac gaccattgtt aaaaaagtga taaaaaaata gtagatatta taacttaaaa
     7681 caagatttcc catcatttta taaaataaaa aagctggata aagagtaagc catggataaa
     7741 aaatattcat cattgttcca tgatgatcga agttattgaa gtttactgga gaactccata
     7801 cattatctag tcctatgatt cgagataaat gaaagtaagt atcctgattg acactatgat
     7861 agattcccat agacatgaat agagggatga cgtaagcaaa acttagaaat aacataaaaa
     7921 tgatatctat ta
//
LOCUS       DAZULG010000118         7815 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000118 DAZULG010000000
VERSION     DAZULG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.122"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            359..640
                     /locus_tag="ACG9CY_002396"
     CDS             359..640
                     /locus_tag="ACG9CY_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938879.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            642..1058
                     /locus_tag="ACG9CY_002397"
     CDS             642..1058
                     /locus_tag="ACG9CY_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938880.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1680..2834
                     /locus_tag="ACG9CY_002398"
     CDS             1680..2834
                     /locus_tag="ACG9CY_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFS4938881.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2807..3250
                     /locus_tag="ACG9CY_002399"
     CDS             2807..3250
                     /locus_tag="ACG9CY_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938882.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4420..5388
                     /locus_tag="ACG9CY_002400"
     CDS             4420..5388
                     /locus_tag="ACG9CY_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4938883.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            5405..5929
                     /locus_tag="ACG9CY_002401"
     CDS             5405..5929
                     /locus_tag="ACG9CY_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4938884.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            complement(6100..7248)
                     /gene="tnpB"
                     /locus_tag="ACG9CY_002402"
     CDS             complement(6100..7248)
                     /gene="tnpB"
                     /locus_tag="ACG9CY_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFS4938885.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(7265..7669)
                     /gene="tnpA"
                     /locus_tag="ACG9CY_002403"
     CDS             complement(7265..7669)
                     /gene="tnpA"
                     /locus_tag="ACG9CY_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS4938886.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT         2810 a         1354 c         1265 g         2386 t
ORIGIN      
        1 aataaaaatt gctggtttta ttcttctttt tgcttttgcg agcagagcaa catggaccca
       61 tgttgcgata ttttggtggc gccccaatcc ccgttttttc gttgaatctt actcggactg
      121 cactcgtttt acagctgatt tttctgaatt ttgaaagagc ataaggaaac gtggtatcac
      181 tttcttacga aaggtgctag ataggctgtg cctatcacgt ttctcagaaa aaaatttgct
      241 atcacttttt tgccattgtg gctaggctat gtatagtgtt ttggcgaaaa atagctatac
      301 aaatactata tttgtatggt atacataaat tatacgctga tacgaaagga aaatttccat
      361 ggacatttta gttcgaaata tcaatcccaa aattattgca agaatagata aacgttgtca
      421 ggaactcaca accaaaacgg gcaaaaaatg gagtcgaaat aaatatctaa aagttctaat
      481 cgaaagtgat tttgaccgtc aacttatgaa ttacaaaaaa gatcaattcg atttattact
      541 cgaaaaattt attgccattc aaacagaaaa tacgaacata ctgaatgact atgttcacac
      601 aaacaaccaa ctcattgaaa cacttatcgg aggaatataa catgagaaaa acttctgttc
      661 aaaaacacgt aaccctttat cccagtcacg ttgacgtttt agattccatc atcgaaaaaa
      721 atccagacgt caacaattat tcagaagcca ttcgtttttt atgtatgagt tatgaacctg
      781 ataccaatac aaccacaacc agtaaccaag caaaactcaa tgccatggga aaagaaattt
      841 ctatcatgac agaaatcatg gcagaatttg ctgatttata tctaaaagat actggtgttc
      901 ttatcggtac aaattctaaa gtgtatcaag aagtgaaaga aaaaattgaa aatcaactca
      961 atccttcccg acgaaatcaa cccaaaaatc gtcaaaaaga aaccaatcaa acctttactt
     1021 ctggaaattt tgacccaaca aactttctaa aaagataaaa aaagaaaccg atccctaaaa
     1081 aaattcctcg atttcttttt agggatcagt cttccagcaa gatttgcaag ttggtaccaa
     1141 cttgcgaaaa aaaagaaaaa tccgcttatg caacttcttc tttttgtatt ttgaggcgct
     1201 accccaatcc ccaagtatcg gttatttaat agcgcggaaa tccaaagaat aggacgtacc
     1261 ctgaaacaaa aaaagactgc tctcacgagc agcccaaaat gagattatcc tattcatcaa
     1321 cataaaaata ttacaaagtg aagaactaga gaaagacaaa aaaagcatat caaatcctct
     1381 caaaaataac aagtctttaa aaaatgacag ataaaaatgc acaaagaact aaaaaagaga
     1441 aatcgcgtaa aatcaagcat tagaagctag taactagcta gaccaattaa aaatcacaaa
     1501 gtgagcgtct ctatccattg gtagaggggc tacttggcat agtcgacatt atgttaacta
     1561 acgctataaa taacgattat gttcccaaaa caatatttta tgaactataa caaaaatttg
     1621 ttatcgcata ttgtatattt taattatagg tgtataccac ttaaatttaa cacaaaggcg
     1681 tgattatttt gaaacccaaa aagaaaattt atctaggtct cctcttttcc ttgatgattg
     1741 gaatttttag tataggaatt acaaatacct atgcttcaga aaattctaat ttcaatatca
     1801 actcccaacc aattgaagaa caagcaatta attttgcgaa aactgatttt gaacactcca
     1861 taacttcaat tcttttaatg gaaaataaca ccgaaactga tattaactat actcttggtt
     1921 cgccatttaa aatgctcgat ggagaaaata cggcaaatag ttctattttc tttccagtat
     1981 tttcagacgc tgataatacg attaaatata tttataccgt caaaaaaaat acaaatggag
     2041 aatattcagg aagtatttca caatttttag caaaagaact tcaaaatcta gtagatagta
     2101 atataacaga tacaacctta ttttttcaag ataatagcat ttattattcc gataatggac
     2161 aaattaaact actttggaaa tcacctttaa gtacagaaaa aaatggtgtt tcttccttta
     2221 cagaaaaaaa tgatcttata tccgtaaata taaaaaataa aattaaaaat ttctctaaaa
     2281 aacaaaattt tcctgcggtt aaacgtgtaa aaagtgtctc ctccggagat aactttatta
     2341 tgattaattg gcgtatagca gaaacccaaa caagttttcc gtggtgtgct gcttacgtag
     2401 aagctgctat cttatgtaac aaaacagatg tacgtgctac ttcagctaga gccattatgc
     2461 attataccta tcctaattta aacaacagtc aaattgaatc aaaaagtctt aatcaagatc
     2521 aaataataaa atatgcaaat tactgtggtg tttatcctaa aaaagtagca cgaatactaa
     2581 acctttcaga ggtccaaaca caactaagaa aaggaaatgc catttatatg ggaactgctg
     2641 gttatggaaa ttatagtaac agccgtcatg cacttgcatt atttgggtgg gtcaatcaag
     2701 gtggttatca aacttactat gtgtggaatc cttggtataa ctacccaact gtaataagtg
     2761 caaatgcagg accagttaca attcctgtac ctggaggtgg ttttacttgg gacaatacca
     2821 ttacaaattg gtaaataaac ccattgcttt agttatgggt ctagtgttag ttcttttctt
     2881 aactagttgt tcaaaaaaac aagaaataca aaagactgat ctgattcctt ttctaggaac
     2941 ttggcaaact aaagatcatt cagaacgtct agtaatcact aatttaaata cttcaaaaaa
     3001 tatagaacaa tataccatct tagtaaaaga gaacaatcat aaaattcaaa tcattgataa
     3061 aaatttaaat ataaatgaac aagttttcat ttacaaagca acaaataatt tactaacact
     3121 gcgctctaat aaaaaacaat tagattacac ttttagtttg gattctccaa aaaagctcgt
     3181 atttattttg acaacgaatg atgggacaga agggacagct aaaccaattg aattttttaa
     3241 agctgattga tgaatagttg tttgatatta caaaacaata atttcaccga aggtgaaaca
     3301 agcgaaactt tacaaaaagt ggctagcatt tgctagcctt ttttgtattg tggagcgtgg
     3361 gttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcaccaa
     3421 atacgtaggt atttggctag caaatataga gcttgccaaa cactttagtg agttttgtag
     3481 aaagtacctt taaaggtact ttcacattac gaacgatacg tccgtaggac gtactgaaag
     3541 ggtgggattt atgagcgaac taatgcaaaa tttggctagc aaaaaaagtt aaaaaatata
     3601 tgctaataat cgagcgaaat gaacactttt tcaagtgagc gaatccgcca tgacaaagct
     3661 gaagcagttt atttcagctt tgtcatggcg tgtgcctaga cttttaaaaa agtcggcaca
     3721 cgagcaaata tctttatcaa gctctaattt gcgttttaag acgtttgaaa cgtctaagta
     3781 tataaatata taagagtagg tttaagagtg cttaaatcga attttagagg gcattcagac
     3841 aactttaggg ttgttagggc ttgccctgac cgcctgtaag gcgctttaag aacaagataa
     3901 gggatttata ctaagactgt cttaaaacgt ctcaaacgaa ggctcatagc ttctcaaaga
     3961 gttccttatg aacattttta gcaaaggagc cggaagcgac tgcgcagtat aatgtgagag
     4021 cagagcgatc acaccttttt aagcgactgt agggagtaag attcccagtt gagaaaaatg
     4081 cgcgaatctg tttggagaaa aaagaagtgg ttttttaggc gggaaatttt gtccccctct
     4141 tttataatct ctttttaaac ctctttttaa acctctttac cgtcggctat atccttactc
     4201 tcccaaggga atgacgcttt tatctacatc gttttgtctg tttatctaca tcgttttgtc
     4261 tgtttatcta catcgttttg tctgtttatc tacatcgttt tgtctgtttg tacattgaat
     4321 tagtacatcg acgatgttaa tataagatgt agataaacag gcattttgta tttcataacg
     4381 tgtatctctt gtgcgagtaa gaaaggagct attttaaata tgtctaatga aatcgtaaag
     4441 tatcatcatg aattaaatac aattccgttg cgaaaattta caccagtcga aatgaatttg
     4501 ttcttttcta tcgtatcaag aatgcgtgat gtaggtgata aaaaagtaca atttacattc
     4561 gagcaattaa aagatttaag caattataaa gcaactgcaa atgttcgatt catagatgat
     4621 ttagaaacaa cgtatgacaa attaatggat ttacgttttg gtagaagaag tgctgacggt
     4681 ttgcaacgtg aacgttttgt tttatttaat caatttaaaa tcgacggaaa atctgatatt
     4741 ccttttgctg aaatacaagt tcatgaaaaa gccttaccct tacttaataa tcttgaagaa
     4801 tgggtgcgct attctctcca acaattcaat gaattagaaa gcagttattc aaaaacaatg
     4861 tttcgattac taaaacaata cagaaccaaa ggtttcgctt atttttcaaa agaagacttt
     4921 catgaattac ttgatattcc taaaagttac aaccaaggta cgattgatca acgtgtgttc
     4981 aatccaatac gacaagaact aaccgcaatc tttaaaggtt taacaatcaa aaagaaatac
     5041 ggaaaaggac ggggaaagcc tgtcattggc tatcaattta catttaaacc tgagataaaa
     5101 aaatcagacg actttaacaa acatgcccca acaaaagaag aacaaaacaa agcattagaa
     5161 ctagctaacc aacaagttca atctgaaaaa gcacctactt cattacaaga aaaaatcaaa
     5221 gaacaaaagc gaaaattgga acttttacaa cagttagaaa agttagaaag agaacaaaat
     5281 acccaaaata aagaaaataa tgcacaaaaa tcagagataa attccgatat tccttcagaa
     5341 ctaaaaaaag acttattaga caatcttcaa aacttattca aagattgagg ggtttttaga
     5401 ttttatgaca gaaaaaacaa tcaaagaact tgctgaagaa ttaggagtta gtaaaacagc
     5461 aattaacaaa aaagtttctg acgaaaatcg aaaactttgg ttttcgaaaa tcggaaacaa
     5521 gtttgtgatt aatgaaaccg gacaaaaggc tataaaatca atgtttttta acgaaaacga
     5581 aaactcaaat caacaactag ttggcgaaaa aacgaaaacc gaaaaccaca aaaacgccaa
     5641 ccaagttccc gaattagaag tcctttctct cctagaacaa cagttaaatc aaaaagatat
     5701 gcaactacaa gaaaaagacg agcaaataaa acaaatgcaa aaattactcg accaccaaca
     5761 acaactcacc ctgcaagcca accaacaaat cgagaaactg caggaacaac tacagttgac
     5821 ttatacggaa gaatcttcgg aaaataaatc aacgactctt tctgaagaaa cagaatcgat
     5881 agaaaaacaa tcaaaggcgg agaaaaaatg gtggcaattc tggcaataaa accagcaatg
     5941 tgaactactc accacttagc taaacctgac ggttcttaac gcttgaagtg ggagcttctt
     6001 gggaatagag catacttgat agcgtatttc tttactaaca gcggtgtctc ttatttacca
     6061 agctatcccc gtagttccta cggttcttga tgattggatc taagccaatc ccatagaatg
     6121 tagtcttaga ccttcggtca gaatattgat actagcattg atatcacgat catgatgagt
     6181 atgacaaaca ggacaagtcc attctcgaat ttcaagagat ttcttgccat ctttatgtcc
     6241 gcactctgaa caaatctgac tagacggaaa ccatttatct actttgatga tttttcgtcc
     6301 ataccagtca gccttgtatt gtaatttagt cacaaacttt gaccaagaaa catcagaaat
     6361 acttttagct aatttatgat ttcgcaacat accttttgtg tttaagtctt caatacagat
     6421 aatatcgtga tttttgatca tttctgtact caacttattc agaaaatcag tacgttgatt
     6481 cattactttt tcatgcaatc tagctacttt gcgtttttgt ttttgataat ttttagcttc
     6541 aaatagattg gtacctttat ttttagccaa taaagcacgc ctagacaatt tgcgttgttc
     6601 acgctttagt ttcttttcca ttttggacgt gaatttatta ttatcaattt tttgtccatc
     6661 agaaagaatc gcaaagtcca taatacctaa gtcaatacca attgtggaat tagttttagg
     6721 taattcgata atttcttctt tacacaacaa agaaatatag tatttaccgc tagaatgacg
     6781 tgatattgta gcagatttga taatcccttt tggttgtcta tgaagcttaa ttcttactaa
     6841 tgacttcaat ttaggaactt tgatgaattt actatcaatc aaagcaactg taccattttg
     6901 attattcgtt gtataactct gaacaggatt tttcttactt ttgaaacgtg gaaatccaac
     6961 ggatttatcc cgaaaaaaat tcttgtatgc tttatctaaa ttaagttggg cattggctaa
     7021 agcaaggcta tcaatttctt tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg
     7081 aaaagtcatt tttttagaag aatcattttt aacttcttca tacgcctttt ttcggtcatc
     7141 aagcattaga ttgtagacct tacggacaca accaaaagat ttggcaaaga agatttcttg
     7201 ttcctctgtt ggatagattc tgaatttgta tgcttttagt tgttccataa agttcaccta
     7261 ctttctattt atgattgttt ccttgatttt ggatatactt cttaatcaca tcaattggtg
     7321 cgccaccagt tgtcagaaga caaaaacttt tagaccagaa catttctttc caaagaaatt
     7381 gtttgactct tggaaagtca cgttttatca atcttgagct ggcactttta taggcattga
     7441 tgaattttgt caattctgtt ttaggttgag ctttgaacat aatatgaacg tggtctttat
     7501 catgattcca ctccactaaa gtaatgtgat acgattctga aattctttca aaagtagttt
     7561 tggcataatc agatatttca tcatcaatca cttgtctacg atatttcgtt actaaaacaa
     7621 ggtggtaata aagaagaaat actgaatgat tatttgtatc taattccatg ttatatcaac
     7681 tcatttcttg tatagactga ttgtaacatg gaacaaaata aaaaggcaat acgccttagt
     7741 tgtcggtctt cgagttacca acggtaaccc tcataccgaa cggcattcat cacccaccta
     7801 tagaggatgg gcgac
//
LOCUS       DAZULG010000119         7782 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000119 DAZULG010000000
VERSION     DAZULG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.123"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(211..909)
                     /locus_tag="ACG9CY_002404"
                     /pseudo
     CDS             complement(211..909)
                     /locus_tag="ACG9CY_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            988..1164
                     /locus_tag="ACG9CY_002405"
                     /pseudo
     CDS             988..1164
                     /locus_tag="ACG9CY_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1340..1909)
                     /locus_tag="ACG9CY_002406"
     CDS             complement(1340..1909)
                     /locus_tag="ACG9CY_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4938887.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1983..2306)
                     /locus_tag="ACG9CY_002407"
     CDS             complement(1983..2306)
                     /locus_tag="ACG9CY_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938888.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2460..3272)
                     /locus_tag="ACG9CY_002408"
     CDS             complement(2460..3272)
                     /locus_tag="ACG9CY_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938889.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3599..4474
                     /locus_tag="ACG9CY_002409"
     CDS             3599..4474
                     /locus_tag="ACG9CY_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4938890.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4488..4811
                     /locus_tag="ACG9CY_002410"
     CDS             4488..4811
                     /locus_tag="ACG9CY_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938891.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4808..5560
                     /locus_tag="ACG9CY_002411"
     CDS             4808..5560
                     /locus_tag="ACG9CY_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS4938892.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVCLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5550..7721
                     /locus_tag="ACG9CY_002412"
                     /pseudo
     CDS             5550..7721
                     /locus_tag="ACG9CY_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2322 a         1534 c         1399 g         2527 t
ORIGIN      
        1 atttgtttac ttctgtagaa tatatacttt gttgttagat aagttgcaca tgtttataga
       61 atctaaataa taaattttta tgtaccgccc ctcaaagtga actgaacccc aaaagttgaa
      121 ctatttaatg gactatctcc agtagaatat cggaaacagt ccattaaata gttcaacttt
      181 tggggttcag ttcaaagagg gggggatttt ttatcgttgt ggaatattta aataccaacg
      241 cttgtcatga aaatcttgtg ctccaccctt agtatttcct tctggatcgt ttgtcgcacg
      301 catcatgacg tatactttct tattagggaa atcacgcata ttgaaagata catgatagcc
      361 aacatttcct aaagtattat aagcttgatt tacgtctggt ctataaatcc catcagctct
      421 tactctagct agttcttttc cagtattgta gtccataatg aaaatatact cgtatttata
      481 attagcaatg tgccatccag ctacatgcaa gtttgcgttt tcgatttctc cgaactgatc
      541 aatgtgggcg taatttgttc catctgtcag cgtagaattt gcagcacctg ctcgagttgg
      601 atcaattact ggtttatcct ccgaagtagt tggattatca tccgtaaaac catgagccaa
      661 atcataggct agtttttctt tacttacacc catttcagaa agataaccgt aaggatctgt
      721 atgatcgccc caaatatttt gtgttaccca taaatgcgat ttgattcctg gttggttata
      781 aggagtgtct aatgttaatg gaataccata tttcattgct gaatctcttg ccaattcaac
      841 gtatacttta tagttctttt caaaagtttc tttatcatgt gtatgttgta actcgatttg
      901 cacaggactt ttggctcacg aggttgtcct actctacatc ttgctgatag atatgctgaa
      961 aggaaaacac caacattatg ttttgctttt tcagctagaa cgagacaagc aggaatacct
     1021 ttagaagata tcaaaggttt tttaggtcat aaagatgttt ccactacaca agtctatgcc
     1081 catatttctc ccgaagtgaa aaaaagatca atgaatcaac tagaaaacta tatagaagag
     1141 caaataaaaa agcactcaaa ttgagtgctt ttttaagctc actatcacta aaactatcac
     1201 taattttatt tataagcgaa catgtttcta tcactgattt tttaaatcta gcgaaaaacc
     1261 cttatcttaa aggcgttcgt tcaattcttt ggcaaggtct tcaaagcctg gcttgcctag
     1321 aagtgcgaac atggttttct tatagctcat gtagaaaaat tttcttgatg atgattttat
     1381 ccatcaattg agattgactg ttttgctctc ctatgatttc agctatgatt gtccgatttt
     1441 gcggttttct tcttaactgg tagttgattt tcccagtttc catcaactcg ctaagcatat
     1501 ccacgactcc gccaaatccg gtctttttcg taataaaacc tagtgctacc attcgttcat
     1561 ctagattttt tgctgtaatc gtcacaccag taatgagcga gtgctgtaat gtttgggcca
     1621 gctcttccat cccttcagca ttggatacga cggaagctgt tttgttaata ggttcttcgt
     1681 ttatttcttt tggttctttc ctctccactt tcactgtttg acgaaggaca tctagttctt
     1741 gcaagagcgt ttcgtattgt tcttgggtca gcatcacgcc agctactttt tcacgattaa
     1801 atatatatac gccagttccc gcgtctcgcg cttgagcaaa tacatccatc ggcgatcgtt
     1861 tcacttctgt gatcgaagat gtgggaactt ctaatttctt taattccaat ggatttcctc
     1921 ctttactaat cgctttttat tatagcacag actgacttgt tttgatcttt tttccaaagg
     1981 ttttatcggt ttttgcctaa ccagtaattg atatcggtaa gaagtctttc gataccaaat
     2041 gaaggatggt acagatacgt tttttgatct tcgttatggt ctagtggaaa aaaatgatcg
     2101 atgatcacta gatctgcata ccgaggcggc ttatttcgag tacatacatg tatagagatc
     2161 cgttttcgat gtcttagcgt tttgttcagt accccagcaa gtcttcttgc ttcttggtgt
     2221 tcatagcgaa aaaaaaagac gattttcact tcaggagggt ggacatctaa ataccctcgt
     2281 tcctggagtg ttttcagtaa aagcatcccc cactctactg cggggtgtgc aagtgcttgt
     2341 tggatcgcta gccgaaattg atatagttta tctttggaat tgggaaattt ttctaatgtt
     2401 tcctgataat aacgaacttc ttctatcagc cggtcatgga taccaattac gtaacgaagt
     2461 cattcttggt aaagtacttt tgtcagcttt gatttgcttt cgctctcaaa ttcgctattc
     2521 agccaaaaca gcgaatcaag cttttcagtc acaatcgtca atttccgata gtcttgtgtc
     2581 tcttctatcc gtttccaccg cttttcagta atctggttac agctcaagcg caaaattgct
     2641 agataatttt tttcttttat tggcaaaatc tgctcctctc taaatagcaa ttcaggaaac
     2701 aattcaaaga atagtgttcg actgtctcct aaaatctcga tttctgccgg atcctttctt
     2761 tgcttcaata attggatgaa catctcagaa aaggactgct ctccatcatc tgcaaaaaaa
     2821 tgcggaaaat ctttcaaaaa gcaaaacagc agatatctta cttttctttc tgaaccaaag
     2881 atctgtgctt ttttataaga aaaagaaagg tggtactcgc caaaccactt tctcaatcgc
     2941 tcttgcgtag aacgatcgta cttctgctcg taaggttcat tcaatagcag atttttcagt
     3001 aaacaaaacg ggggtgattg gatcagaaaa acaaacaaga gataaggttc gctatatcct
     3061 tgatcataaa ctaactgaac ggttttctta tgaagaatca gctgaatggg acagtcgctt
     3121 tccgccaaac gtttcagatg ccgatccact gttttaacat cgattttcag ttcttcagct
     3181 aaatcagctt ttcgccagat caaatctttc attagattga ttagtgaaaa taaattctgt
     3241 gcattttttg ataatagttt ttgccatctc atcgatcatt tctcacctga tttctaaaca
     3301 tattgtttga tcaaaatggg ttcactccta aaaagataaa aaactccctt tctatttatt
     3361 taaatacgca aatcttcctc attatttttt attttactca atttaatcgt attctcattt
     3421 caaaacgaat gtactttaaa aaaacataat aagatcactt tgcttgtagg atgtattccc
     3481 ttccattaca atgaaataaa tagcgatttt atgagaaggt gatgtgaaaa tggaagaaaa
     3541 aatagaagaa tccaaacaaa aaatcgatca gttgatataa gaattagaaa aagcggtcat
     3601 cggcaaacgt tcaactatcc aattgatgat cgtggcccta ttagcaggtg gtcatgtctt
     3661 attcgaagat attcccggtg taggtaaaac gttactggtc aaagcactag caaaagctgt
     3721 acagggaacc ttcagcagag tccaatgtac tcctgatctg ctgcctagtg atattcttgg
     3781 tttttccgta tacaattcac aatcaaaaga gtttgaattt cgtccgggac cgatctttac
     3841 cacgatcttg ttagcagatg agatcaatcg tacgactcct cgtactcagt cagcactttt
     3901 agaagcaatg gccgagaatc atgcaacgat cgacaacaat acataccctc tagacaatca
     3961 tttttttgtc ttagctacgc aaaatccaat cgagtacgaa ggaacatatc ctttgccgga
     4021 agcacagctc gatcgttttt tattccgact acaaatcggt tatccttctt ttgatgacga
     4081 acttttgcta cttttggata aattcgaaaa agtgttggat aacttaaatg aagtattgaa
     4141 cagttatgaa atagaagaaa taaaaacaaa tgtggcgaat gtctttgtca gtcccgaagt
     4201 ggcatcttat gccttgcagt tagtgtcagc aacaagaagt catgctgcca tccagcttgg
     4261 catcagtcca cgaggaagcc tttcctttat tcaggcagca aaagcttatg ctttaatcca
     4321 tgggagaaat tacgtcacgc ccaaagattt gcaggattta gttccttacg tgttttccca
     4381 tcgcctgatt ttttacgatc gttcattgaa ggaagacgaa aagaaacaaa tcattcatac
     4441 tcttgttgca caaattccta ttcctgtgag gtagattgag gtagacaatg gaaaaaatca
     4501 aagattattt cattaatttt gtgttatttc tggtatattt catcattgtg ctttttgctc
     4561 ttgtattcaa caattcttta ggctggttct tgttggcctt ttttactctt tatgtatcat
     4621 ggagcttgat ttcattaagc agctttctaa agaaactttc ggttacttgt acattgcagc
     4681 cactcgttta tcgcaaaaaa agtatgcagg ttattttttc catagcaaaa aagagaactt
     4741 tttctttacc tctccctcgt ttacaaatcg gcttcccagc aatctttgca gaaaaaaaca
     4801 agagattttg attcttacaa ataaaccgca agaagtccaa ttgctttggc agccaaaaga
     4861 aagaggatgc catgaaacat taactgtcat ttttacagcc tatgatagtc taggactttt
     4921 cagaaaaagt gcaaaaagag agatctcttt tcctgtgact gttctgcctg ctttttgtaa
     4981 gattcaagca gaaaaattac agagagttct ggaaaaaaaa cagcagctga acgcgtacca
     5041 aagaggtctt gattttcggg aacatcgcgc ttatcgtcca ggtgattctt tcaaccgaat
     5101 cgattggaaa ttgtctgctc atactcaaga gtggttatac cgtgaatacg attatcaaga
     5161 agaagaatgt tctcctcttt tttgcttttg gggttcgccc agtccaaaat ttgaacctct
     5221 gctagacttg tatttttctt tatggcattt gagaaaagaa aagcagccgg aacttctgat
     5281 attaggcaac cgcgcctttc atggcaaaga ccccggtcat acctattttg cttctttgga
     5341 agctggcgat gagaaagctt ttttcgcaca cttgaaaaaa tatgcaaaaa aacaaatcgt
     5401 cctctttgtt cctgagaatt ctcctgaagt ctcagaagct attgaaacat tgtcaaaaga
     5461 tcgaacggtt tgtcttgttt atttcgtaga aaaacaactc atggtccaag aaaaagacaa
     5521 gatatgttca ttgccaggag gtgaatggat tgacgattaa acgggaaaaa tggctgcgtg
     5581 gcgtttactc ctttgtgata ctaagtgtag gtgctccgat atttgtagta gctaatcaat
     5641 tgcaaaacag ctaccttctt atcctaacta ttggtgtcct atgccttatt gtctgctgta
     5701 ttcctaaaaa atgggtacgt ttgctgcttt ctttgccagt aatgataggt tgtttgtttt
     5761 tatattttcc ttcgaagaca ttttccatat tatggatctt acagtttttt tcgtcagtca
     5821 cagaggaatt cttccatcta tcccaaggta aactttatta tttgccagaa aagacagctt
     5881 tttttctcat aatgttgctg atctatttat ttattacatt gaccgtggat tacgactatt
     5941 ggtacgcacc ctttctttct ttgatggctt acttcatgat gcttaccgtt tttcggaaaa
     6001 aagatttact gtacgaaatg attgcggtag taactgtttt gttcgtgtat ctggcagcaa
     6061 ggcaattttt ctccatccgg ttgttgtcgg gaaacactcg attccagtca ttgactacag
     6121 ttctgcttat ttgtttctta acttttagcg ctatcttgcc tttatatctt ccgaaagtac
     6181 accaatctct cgaggaaacg agtgaaccga tccgagaata tcttaatgat gaagggcttt
     6241 atgacacact ccatgagtat caattaaccg gaagtgcaag aacaggcttc agtgagaatg
     6301 atgaacagct tggtggacct ttatatgata atggcgaagt tgtttttaca gcgaaccaaa
     6361 aaggaaatgg ctattggaag atcgaaaata aaaatcatta caccggttct ggatggatag
     6421 aagatcagga cgcacccatc caaccaatcc aagaattccc tgttgacatc acgagctacc
     6481 aaaccgttat agaagacaaa gaaaacatta cattgaccct aaaaaaacaa gttttcttac
     6541 cttatcctta tggaaatact accttgactg atttttcatt agctagtgga caaacgcttt
     6601 actatttgcc tgaaagccag cgattcattc ctgtcccgaa tgaatcgaca cgttctgcaa
     6661 ctttacaaac agaaaggtta gtttttacga gagaagaatt aaaaagcagt cagttaaaag
     6721 ctgatgattt agaaaatcag cagacaaatc ttcaattgcc aaaaagtttg cctaaacgta
     6781 ttgaggactt ggcggtcgaa ttgaccaaaa atgaagcaac tttaattcgt aaagtcgaag
     6841 ctgtctaaca gtatttaaaa acaagcggaa atttccgcta ttccaaaacg gataccgggc
     6901 atactccaaa agaacacgat tatgtggatt atttcctctt tgattcaaaa gtcggctatt
     6961 gtgacaactt ttcaacttct atggtcgtcc ttcttcgaac acttggtatc ccagctcgct
     7021 ggacaaaagg ttttacaaat gggctcagaa caggcacaga taatcaaggg aatgaggaat
     7081 acagcattct caatagtcat gctcattctt ggccagaggt atactttcct ggatttggct
     7141 ggcttccatt tgaaccaacc ccttcatttg ccaatccaat gggttcacaa ccagttgctg
     7201 aaacaaccga aaccgtccag cctgctatcg aacaaccaaa tgaaagcgaa aatatggcag
     7261 aaactatttc cagtgagaga tctgaggtca ttccatcaga agaaacaaca ctcacaccag
     7321 cttataaaat cgtcttattc agccttggag tgtgtttatc cgtcttgttg ttctttctat
     7381 tgttattcag aaaatctctg cgctggacaa gttatcttat ctatcttcat ttttttgctt
     7441 ctactaatac gattgcatat caaaaacttc ttaacccttt ggaaacattg gtaccgcgca
     7501 aacccgcaga atctttgcag gaatatagcc tgcgaataga acaagaattc aagctagatc
     7561 tgcgtttcat tcgattaact actcaatatg aagcagaaat atatggacga caagcagatc
     7621 cggtaaaatt cgataaaaaa acctatttag aaataacgca tataataaga aaaatggtat
     7681 acaaaaaaat tctgacaaaa gggagccgct tcaaaaaata agataaaatt tctggagaat
     7741 ctacttctgg aatgccgaga accagctgat tgagtatcga tt
//
LOCUS       DAZULG010000120         7728 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000120 DAZULG010000000
VERSION     DAZULG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.124"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..343
                     /locus_tag="ACG9CY_002413"
     CDS             <1..343
                     /locus_tag="ACG9CY_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4938893.1"
                     /translation="PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSA
                     LGIPLAAKTGTAEIKEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRAS
                     ELLQYLNQNYQ"
     gene            complement(452..1603)
                     /locus_tag="ACG9CY_002414"
     CDS             complement(452..1603)
                     /locus_tag="ACG9CY_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4938894.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(1757..2287)
                     /locus_tag="ACG9CY_002415"
     CDS             complement(1757..2287)
                     /locus_tag="ACG9CY_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4938895.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(2386..2736)
                     /locus_tag="ACG9CY_002416"
     CDS             complement(2386..2736)
                     /locus_tag="ACG9CY_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS4938896.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(2893..4422)
                     /gene="ahpF"
                     /locus_tag="ACG9CY_002417"
     CDS             complement(2893..4422)
                     /gene="ahpF"
                     /locus_tag="ACG9CY_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS4938897.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(4432..4995)
                     /gene="ahpC"
                     /locus_tag="ACG9CY_002418"
     CDS             complement(4432..4995)
                     /gene="ahpC"
                     /locus_tag="ACG9CY_002418"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS4938898.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(5139..5801)
                     /locus_tag="ACG9CY_002419"
     CDS             complement(5139..5801)
                     /locus_tag="ACG9CY_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4938899.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            5985..7631
                     /locus_tag="ACG9CY_002420"
     CDS             5985..7631
                     /locus_tag="ACG9CY_002420"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4938900.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            7622..>7728
                     /locus_tag="ACG9CY_002421"
     CDS             7622..>7728
                     /locus_tag="ACG9CY_002421"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4938901.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMD"
BASE COUNT         2548 a         1494 c         1361 g         2325 t
ORIGIN      
        1 tcctaaattg attgcagata aagagacaaa agataagaag aatgtcatca gcgaaacagc
       61 agtacaaacg atcgtgccag atctgagaga agttgtgcaa gatgtaaatg gtacagcaca
      121 ttctctttct gctttaggga ttccgttggc agcgaaaact ggtacagcgg aaatcaaaga
      181 aaaacaggat gtaaaggggc aagagaacag tttcttgttt gctttcaatc ttgataacca
      241 aggttatatg atggtcagca tgttggaaaa taaagaagat gatgattcag caaccaaacg
      301 agcatcggaa ctattgcaat acctcaacca aaattatcaa taaaaaattg ctttttaagt
      361 acagtaaaag gactgtggcg ttatcgtcac agtccttttt tcaaataaac ggttttcacg
      421 atgtctcttt cacaacttct ttttgattta ctcacacttc ataaactttt tttgtaaaat
      481 atttgagaac aagtggagaa ataagtgtac ttaataaaat cacgataact aaaggtgaat
      541 agtactgtgg ttcgatcaat tgactttgct gtccaatttg taaaatgatc aaagccattt
      601 ctccacgcga aatcatacca gcaccgacca tcaatgaact atgttgagaa aaaccggcaa
      661 tctgagcacc aaaataacct ccaagcaatt tggtcaaaat cgctacaagc gtcaatatag
      721 aaataaagag tagttgttca ctaaagtcag aaaaatcaac ttctaatcct acactgacaa
      781 aaaatactgg tataaatact gcatatccta aagcctctac attgtggtat acttcttctt
      841 taacctttgt ctgactgaca gctatcccag caaaaaaagc accaatgaca gagctaagcc
      901 caacaagatc cgctacgtaa gacattccta aacaaataac tagcgatacg atgataatag
      961 aagaatttgc gtacactttt tctgcaaatg aaagtaggaa aggcgcaatc catttgacta
     1021 ataagaaaat aaaaacaaaa taaattagct gttcaatcaa aacaagcggt aaagaagcat
     1081 tgctagaagc ttcccccgtt agaaaagata ggctgaaact tagaactaaa acgactaaaa
     1141 tgtcatctac cacagaagca cccaaaattg ttgatccttc ttttgtattc actacgttta
     1201 gttcttttaa tacttctact gaaatactga cagaagttgc cgcaagaata atcccaaaga
     1261 aaatcgcttc atttcccgaa acttggaatg ctttgcctgt caaccagcca aaaacaaccg
     1321 ggaagatgat cccaagcaaa gcaacgaaca taccaggtcg gaaatatttt ttcaaaagag
     1381 ataaatcact ttccagacca gctaaaaaca ttaataagat cacaccaatc tccgaaaagt
     1441 catggactag aatatcaggt ttgacccaat tcaatccagc ttggcctagc aaaacaccaa
     1501 caagtaattg tccaatcact gctgggatac ctaatctccg cgagatatgt gagccaatcg
     1561 ttgttgaaat caatatcagg cataaaatac caataaattc cattctcatc attcctttgt
     1621 ataaaatata gcccgaactt agcataacat acattattga aaaaatagga aaatatactc
     1681 acaaaaaaac caacaatcca aaagattttt ttggattgtt ggtttttata cggtctattt
     1741 taataactaa aaatgattaa cgtatagtca tttttcttat tctgatgaaa ttgataatct
     1801 cctggatgcc catgaaaatc aaaataaagc cgaatatttg gaatagaagc atcaaacttt
     1861 tgaatggatt gaacaataag acgaatccgc caatcaacaa cagaatacta ctataaacac
     1921 tccaactttt agcttttgta ttcatgctga agaacaactg gaacaagctg ttgatgataa
     1981 cagctaatcc aagaagaatc ggcaggatag aaacgattgc aggtgcaaaa gcatagacga
     2041 tcagagctaa gatcaggaaa aaaagaccac tgaacagacc gatactccaa gaacctgttt
     2101 tccttttaaa acggtaagcc tcaaaaagat tcaacaaccc aagcaaaaca agataggcgg
     2161 tgatcagata accgataaaa tgaaaaacag cagaagggtt tagcacaatc gcaatcccag
     2221 aaataatata aacagcagct cttaataagg cgtatttttg aatattttgt ttaatcgtct
     2281 caaacatata agaaaacctc ctttgactta ttttaacgtg aactagtcat tttaccaaat
     2341 taaatgttac aaaatgagcg aaggagaaga atatctctgg aaaaatcaac ttctgaggat
     2401 cttttccatt tttttgcctt ttgctagctc atcgatcatt ttatctaaat aacgaatttc
     2461 tttcatcaag ttatcctcga tgtcttctac tcgtacacca caaacaacac ctgtaatcaa
     2521 ggaacgtgaa ggattgggga caggggcttc cgcaaaaaat tgttcgaaag tcacttgttt
     2581 ttgtagttga tattctagtt ctgtttggga ataacccgtc agccaacaaa tgatttcatc
     2641 tacttcctct tttatacgat cttttctctc cgcttttgct acatacatag gataaacgct
     2701 tgcaaaactt gtcgtaaaaa tacgattttg cgccataatc agtcaccttt ctttttttca
     2761 aattatttac tacttatagt atataagaaa aagagaagaa aaagcgatta ttcaaaaaaa
     2821 caaaaaggat tgtgacaaaa ttcttgtcac catccttttt tccgctctct gactttttac
     2881 aatcttcata gctcaagtgc gcatcagata atcgaatgct cctaaagctg cagtagctcc
     2941 tgaacccatt gaaacaatga tttgtttgta tggactatct gtacaatcac cagctgcgaa
     3001 aacgccgggg atattcgttg cgccattttt ctcggtcact atttcatttc ttggattcat
     3061 ttcgatcgtt ctatctagcc aatccgtatt agggagtaag ccgactagaa tgaatactcc
     3121 tgaaacagct agtgtatgca tctccatcgt acggcgatcg atataggaga gagattctac
     3181 ctgattcgta ccgctgattt ctttcgtcgc tgcatgtttg ataacagtga cattcgcaag
     3241 agagactagt ttatcctgta agatttgatc tgcttttaat tctggtaaga attctagaac
     3301 atatacatgt tttgctaacc cagccaaatc gattgctgct tcaacaccag aattaccacc
     3361 accgataaca gcaatctctt ttcctttaaa taaagggccg tcacagtgtg ggcaataagc
     3421 aattccttta tttttaaact ctttttcgcc tggtacattg atagaacgcc agtgcgcacc
     3481 tgtcgcgatg atggcggttt ttgctttaag tgatataccg ttagctaagc caatattaat
     3541 aaaatgttgc tctttattta aggaaacggc ctgttgattc atcataatgt cgatagggta
     3601 actacgcaca tgactttcta cttgctgcat caattttgga ccttctgtgt aagaggtacc
     3661 aatcatattt tcaatcccta gtgtttcaac gacttgtccg ccgaaagaat cagcgacaag
     3721 tcctgtacgg attcctttac gtgctgcata gatagctgca ctactagctg ctggtccacc
     3781 gccaatcact aatacatcat aaggttcttt ttcaaacaac tcttcttcaa tcaaagggcc
     3841 agtgattttt tcaacaattt gttcgatcgt catgcgtccg ctactaaatt cttctccatt
     3901 taaaaacaca gttggcactg ccatgatttt tttatcatcg atttcttttt ggaacatccc
     3961 accttcaatc atcgtatgag taatatttgg atttaataca gctaatatat tcaatgcctg
     4021 tacaacgtcg gggcaattat gacaagtcaa gctaacataa ctgtcaaaat gcaatgtttg
     4081 tgttattttt ttgattttaa gactcaaaga ttcactgatt ttcggtgccc gtccactaat
     4141 ttgtaacata gccagtaaaa aagaggaaaa ttcatgaccg agggggatac cagcaaaaac
     4201 gattccactt tctttgccaa ctggattgat agaaaaacta ggtgtgcgca tcgaatctct
     4261 tttttcccaa gagattttag gagacatttc tgctatttcc ttcgtaaatt cagctagttt
     4321 ttgactattt tcactattat ccaaactaac agaaaaaaca ataggagatt caatcagtgc
     4381 taaatattgt tcgagatttt gtttagtttc tttgtctaac atatccacca cctaaatctt
     4441 tccaacaaga tcaaaacttg gtttcaatgt ttcgccactt tctttccatt ttgccggaca
     4501 aacttcacct gggtgggtac gtacatattg agcagcgcga atcttatcaa tcagtgcact
     4561 agcgtcacga ccgattccat ctgcattgat ttccatagct tgaacgattc cgtctggatc
     4621 aataataaat gttcctcgct gagctaatcc ttgctcttca tccaaaacat caaaaatacg
     4681 agaaatctga tgagacggat caccgatcat cacgtattcg attttaccga tagcatctga
     4741 tgtatcatgc catgctttgt gagtaaagtg ggtatcggta gagcaagaaa agacttcaac
     4801 acctaaagat ttcaatgtac tgtattgttc ttgtaagtct tctaattcag taggacaaac
     4861 aaaagtaaaa tcagctggat agaagcaaag aatactccag ttccccataa gatctttttc
     4921 actgacttct aaaaattctc cttgatgata agcatgtgca ttaaattctt cgattttttt
     4981 cccaatcaat gacatagtaa aacctccaat aataattatt tatttataat tattgtaatt
     5041 tagttggagt tgcaaagtca atggatattt tgttatttaa taaagattac tcaaaaacga
     5101 aggttaatta taaaaataga agaggtcgca agaaaatgct actgctggaa atactcggga
     5161 taacgttgag taacagtttc ctgttctttc agcatcatgt gtaagtgcaa tcccgtttga
     5221 aatcgagctt cttctggttt tttatcaact aaagccttga gtaaatcata atgttgttcc
     5281 atgatcgttt cccatggaag cccctgaatg gtcactcgta accagcggaa acgttctaaa
     5341 tgggtattat ggtcatctaa ccagttccaa acttcttttc ttcctgcaat ttcgaaacaa
     5401 gcttcatgaa ataaattatc tgcacggaaa aaagcgcgtt cgtcatttat tttgactgct
     5461 ttttcttgtt cttctaaaat cgtttctagc actattcgtg tttgtgtgtc caatttggaa
     5521 caacattcaa tcatgatttg ctcttccaac tgttctcgga caaagcgagc attttctgca
     5581 gaaacaagat taattttgga aacatacgta ccactttgag gtatcgtata cacgagttct
     5641 tgctgacgta gttgaatcaa tgcttcgcga atgggagtcc ggccgatagc aagatgttcc
     5701 tccagtgcct tttcagatat tttccttcca ggttctaagt cagcatagat aatttgttta
     5761 cgaatcgctt gataagcttg tgattgcaag ctgcttttca tctttattcc tccaaaatca
     5821 tttctatgaa cgtagtataa cagaagaggt ctgaaaaaca atctggtatg tcacattaac
     5881 tagtaaattt gtataaatag taaaattttg gttgacatag cgatttttat tgcataaaat
     5941 tcacttgaat acaaaaacta atatgttagt ttaggagggg aaaaatgatt gatttaaaca
     6001 actggaggaa ctttatccct gaattatctc aaaaagaagt agaaatacct cattttgacc
     6061 gtgcatcatt aagagaaaat ggacaaaaaa atcctgtctg gatccatttc ggtggcggaa
     6121 atctttatcg aggatttcat gcagaaatcg ctcaacgttt agcggatcta ggcgaattgt
     6181 ccactggtgt cattgtttgt gaaacattcg atgaagaaat aattgaaagg gcttaccaag
     6241 attttgatga tttgattctt gaagtggtca tgccaagcag tggagagttc gtcaaacgta
     6301 tattagctgc gacagctgac agtctgtatt gtcatccaga ttgttctgaa cactttgata
     6361 agatgattcg acactttgaa aatcctagtt tacaattagt tacttttgct attactgaaa
     6421 aaggttatcg attgaaagat acaaataata aatatatagg accgattgca gtcgatataa
     6481 aaaatggtcc aaagaagcca tcacatacaa tgagtattct tgctgtgcta ctttataaac
     6541 gttttttatc tggagcttgg ccgattgcac ttgtctctac agataatttt tcacaaaacg
     6601 gtagtcaatt acgtacttct ttgctaacga tcgcaaaaga atggcaaaag gaaggttatg
     6661 tacaaaatac atttatccaa tacttatcga atgaagaaat tgttgcgttt ccatggacga
     6721 tgattgatcg tatcaccccg aatccttcct tgtcagttag caaaatgttg gcaaaagagg
     6781 gcttttcaaa tatgtcgatt ttccaaaccg caaaaggaaa taatattgct ccatttgtca
     6841 atacagagga catccattat ttaatcatgg aagatcattt tcccaatgga cgtccgaaat
     6901 tcgaaaaagc tggggtattc ttgactgatc gtgcaacggt agataaagca gatacgatga
     6961 aggtgacgac ttgccttaat cctttgcata cgtctttagc tattatgggt tgtttactgg
     7021 ggtataggag catagctgag gagatgaaag atccagattt atcgctgcta gtttctgaaa
     7081 tcgggtacaa tgaaggcctc ccagttgtgg agtctccagg tatcattgat ccaaagatct
     7141 ttctggaaga agtaattact gtgcgctttt ctaatccaaa tattcctaat atgcctcagc
     7201 gaatcgccac ggatacctct caaaaggtgg cgattcgtta tggtgagacg attaaaaaat
     7261 atatggaaat agaagttaaa gacccgcaga cacttgtatt cataccattg actctcgctg
     7321 cttggcttcg ataccttatt ggcattgatg atcaaggaaa accattcatc ccgagtccag
     7381 acccactatt gacagacctt cagcataagc tttccgatat tcaactgggt acgcaagata
     7441 catcacatat tcatgagaaa atggctccta ttttgtcaaa taaagaaatt tttggtgtag
     7501 atctttacca agcagagctt ggcagaaaaa tagaagaaat gttcatcaag atggtagcag
     7561 gaccaaatgc cgtacgaaaa acattacagg attacttaca agaacatgga ggaaatttat
     7621 catggaaatg acttttagat ggtacggatc acaggaagaa aagattacct tagatgaaat
     7681 cagacagatt cctgcggtca aagggattgt aggcacattg atggatat
//
LOCUS       DAZULG010000121         7615 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000121 DAZULG010000000
VERSION     DAZULG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.125"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..2340
                     /locus_tag="ACG9CY_002422"
     CDS             13..2340
                     /locus_tag="ACG9CY_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFS4938902.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2337..3251
                     /locus_tag="ACG9CY_002423"
                     /pseudo
     CDS             2337..3251
                     /locus_tag="ACG9CY_002423"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3324..3839
                     /locus_tag="ACG9CY_002424"
     CDS             3324..3839
                     /locus_tag="ACG9CY_002424"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFS4938903.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3967..5265
                     /locus_tag="ACG9CY_002425"
     CDS             3967..5265
                     /locus_tag="ACG9CY_002425"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4938904.1"
                     /translation="MHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYP
                     SKDTAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLS
                     LPFYNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQ
                     FVTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQ
                     KETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHF
                     IKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKL
                     ISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQ
                     PKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5471..6643
                     /locus_tag="ACG9CY_002426"
     CDS             5471..6643
                     /locus_tag="ACG9CY_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4938905.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6805..7479)
                     /locus_tag="ACG9CY_002427"
     CDS             complement(6805..7479)
                     /locus_tag="ACG9CY_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938906.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
BASE COUNT         2573 a         1439 c         1519 g         2084 t
ORIGIN      
        1 ttgagaggag ggatgaaaat gattaagaaa actcaaaata aaatccaacg tatatgtcaa
       61 aaacattttg tttctagact caaagctgta tctaaaagaa aatacttaca tcccattatg
      121 atttcgatca tcttgatttt tactttctta gggagcttat caagtattta tgtaaacgtt
      181 tatacagaaa cgtcaaattt tgctaaccaa acgacagagg agacaaatag tccaccctat
      241 tctgaaatac agtccataga aagcaccaat cagtctagca acaacgagac aacctcaacc
      301 acatcctcga ctcaactgat tgatccgaca gaaaaagaaa tcacagcagc aaaagcagca
      361 ggggagaaag cttattcaga aacaggtaga agccagacat ttagtactgt tgcggccact
      421 gcgaatttca acggacatac cataccagat ggatatacag tgatatatgc caaagatgga
      481 gtcattgttg cggttaacgg atcgatgagt gcggataaaa ctgcgttaca aggcgtaacc
      541 aaagcaatcg cttttattga taaccgtcca gatatccata atctgtttat tggctccaca
      601 gtaaatggta ctactatcac attcccaaaa agtatctata tgggggtggc taccgctgga
      661 aatacgatcg attcagttac acccaatgcg gataaaagtt attcttttac accctcaaca
      721 tggggagggc ttcctgccgg aggaggtatg agtacttttt caatcggtat acagttagca
      781 aatgatattc cattcacggg gtactcgatc aaaaatgggc agatcgttga tggtaaggga
      841 aatgtttcaa attttccaac ctatcaagca gcaacggttc aggtcaatgc gaatcggaca
      901 ggaacttttt cttatacagt caatggtgga gcagcaaaaa tcggaacgta cgggactaca
      961 ttatctggaa tccatgtggg tgacaaagtg gttatcactc caacagcagt aacgggctat
     1021 tcttttgcac aaacaaacgg ttctttcaca gccctagaaa gtaacgtcga tacggtgacc
     1081 tatacaggca aacaaacacc aatcactatt aagtatcaag actattttgg acagatgatt
     1141 gctccttcta aaataatgaa tcttacttac ggaagtgctg cacaagacct gacgactaat
     1201 acgcctacta tcaacggata tacctttaca gctgtttcag cgacagaaac aaagaaccaa
     1261 tcagcaatga gtgttttagc tagtttagat acaaatggaa atgtaatcgt aaaggacgca
     1321 aatggaaaac agatttctga agtcatccta tattataaga caaatgcaac agtatcaatc
     1381 aatgcaaatg gcagtaagta ctatgatggt ctttctgttg taccgatcgt taaatatgcc
     1441 tttaatgata aaacagaatc aacatccctt ttaaacaatg atctcagtat tgatcaaatc
     1501 acatggaatc caactgattt caaagcgatg aatgaaaagg gagttgaagt aactgcgcct
     1561 actgaaattg gaacctatac gtcttggcaa ttgacaaaaa gcggactagc taaactagct
     1621 gccagaacaa actatttatt tactatcgtg cagacatctg atgtctatac gattaagcaa
     1681 atcagcggtg cggtagagct cagtggcagt aagacatatg atggaaaagc aggtgtcccg
     1741 tcaatccatg tcaagctagc agagggtgtc actagttcgc ttactccagt acaagtggca
     1801 cttagcagta tggattacac agcagatgca caatcagcaa aaaatatggt taatgctggc
     1861 agctatacaa ttaaactaac gaatagcgga atagacaaag tcaaagcagc aaatcccaag
     1921 ttgtcattta ctgatatagc caacacatca ggaacttata ccatctataa agctaacgca
     1981 gtgattactg tcgatgacgc tgcctttaat tatgatgccc aaagtcactc gatttcggtt
     2041 ggaaatctcc acgttaccgg tgttgtctta ggcgaatcat tggattatac cctaacagtt
     2101 aacagccgga cagatattgg atcacaaata gtaacgatta gtttaggcga aggaagtgta
     2161 aacaataatt atacgattat cgcaacagat accgctaagt tgacgatcaa tccgactctc
     2221 ttgcttcctt cgacaggtgg gataggtcta ctgccctttg tctttttagg actgatcttt
     2281 attttttctg gcttctttta ttttatccat cgtaagaaaa aagcaggtga acagcgatga
     2341 aaaaaacaaa aaatgtacgt gtggatatat ttgctgttct cttttttatc ctactggtct
     2401 tttttatagg ttctccgact gtgtcagcta gagaatcacc tattacattt actttgcata
     2461 atcgaatgtt cgaaagcgat aagatgccaa gtatcagtca aaacgacgga ataaatatcc
     2521 cggataactt tcaaggagta aaagggttga acggcgttac tttcgctata tatgatgtct
     2581 cggatgaatt ttacaagctg cggtcagaag gaagtagtgt ggaagacgca caaagaaaac
     2641 tagcacaaaa aagtgatagt gaaaaaatac tggcagaaaa tgtgacaaaa acagtagacg
     2701 aggaagaggg cattgcttct tttagtgcat cggacaaaga tgagcaaggt cgtgacgctg
     2761 tttaccgatt tgcggaaata aagacttcag attaagtaaa agaaaaatca gcaccatttg
     2821 tcgttgtgct tcctgtgaca gattcatcta atcataaatt gacgaccatc cacttgtatc
     2881 caaagagcga acaaaaaata cctgctgcac tcacattaac aaaaacagta gagaataagc
     2941 agacagattt tgcagatggg gataaagttc cttatctcat caccgtaaca attcctgaga
     3001 acatcaacga gataggaacc tatacgatca aagatacagc tgatcctcag ttatggctag
     3061 aaccagaaac gatcgatttt ttgattggtg gagacaagat tcatacattt cacacacaaa
     3121 aaacaaaaca tggttttgtc ttatcagatt ctggaaaaga tcttagttcg tttgctggga
     3181 aaaaattaac gatccgttac caaatgacat tgaaagaaaa cagtgaaaaa acgacatttg
     3241 ataacgatgt acgtttgacg actgacaatc aaacagtaga aacacatctt gccatccaaa
     3301 caggaggcaa acaatttgtc aaagtggatc ggcaaacgaa acaaaaacta gcagatgctc
     3361 aatttctagt caaaaaaaat gaccaatact tagcgcaaga aaacgggata aatcattggg
     3421 tagccaaaga tgattcaaga gcaacgattt ttacttccag taaagatgga acattttctg
     3481 tccatggatt gtcttttgga agttacgaac tagtggagat taaaccgcct aaaggatata
     3541 tattgagtca atcaaccatt ctctttgaag ttgaaaaagg cagttatgat ccgcttcatc
     3601 ctataccatt ggaaatcatc aacgaaccaa aagcaaatcc gacgaataat ggaaaaacgc
     3661 cagttcagat gcagacaaat ggtccatcaa atgaaaaaaa tggaggaagc tttccaaaaa
     3721 caagcgaaga gatgcttgga ggtttctcaa ttttagggtt gttgctagtc ctttcaacag
     3781 ggacagcttg gttttataaa aaacaacaaa aaggaaacaa taggagggaa ataaaatgaa
     3841 aaacatcaaa acaaaaatat ggacgttttt aggaactgct atcatgctgc ttcctttcgt
     3901 tctcggccta ggaactgcag aggtttctag gccgtatcac ctacaccaga aaatgttacg
     3961 gttaatttgc ataaattgaa attcacttca gcaccagaaa atcaaataaa caacggaacg
     4021 gaattgacat ttccgaatag cgaaccgttg aacggcgttg aattcaacgt atatgacatc
     4081 actgctacgt actatccaag taaagacaca gcagttccag cagacgctac acccttcgca
     4141 agtgtaacga cttctggtga aggattagct aacctgactc ttccaggtaa atctgatggg
     4201 aaagatgctg tatacgtctt tgtagaaaca ccaaagcccg gtgtcgaaac gtcgccaaat
     4261 atcgtgttaa gcttaccctt ttataacccg aattcatcag accctgacaa acctttagac
     4321 acgattcacc tatatgctaa aaatgtcatc aaagaaagca atatcaaaat ccagaaagct
     4381 gggaactttt caggggtaga taagttagca ggggcgcaat tccagatcaa aaattcagag
     4441 aatcaattcg tgaccggttt tgatgaaaac actggtgtcg ctctctatga tcctgctgag
     4501 tcagcgttga cattcacaac tgatactgaa ggatccatca gtgtcaatgg attaatagac
     4561 ggcaattata ctttagttga aacaaaagca ccagatggtt atcaattaac caatccacaa
     4621 aaagaaacta cttttatagt aaaaaacgga aaattgcaac aaacaaccgg tatcatccat
     4681 ggcgcagatg aagccaatct tgggaatcca aacggtgtag tcaatcggga aaatacaact
     4741 acggataaca cgattactgt agaaaatgtc caaaactacg gtgatttcca cttcatcaaa
     4801 caagatgtga acacgcaaga aaaattagcc gatgcagaat tttctattct aagagcaaaa
     4861 gatccaactg ttaaattaat gaaaaaaata gcaaatcttg cctcaggaga atacccatat
     4921 gcttggtcag atgaagtcac aggttctgac tgggaagaag tgaaattgat ttctgattca
     4981 gaagggaaat tttcaatcga agggctgaaa caaggggcat actgccttca agaaacaaaa
     5041 gcaccagaag gctacgcatt acctaagaat gaagctgcgt acactgaatt tacagtagat
     5101 gatgatccaa caacttctga tgaaacaacg atcgataacc aacctaaagg aatcttgcct
     5161 tcaactggtg gtatggggat catcgtattc atcttggtag gtactgcttt agttggagga
     5221 gcagtgatct acttcaaaaa acgccatcaa gaaacagaag cttaaactat tggaaacaaa
     5281 aatcatacga taaataaacg atagtgattc aaatggggag aaaggttgtg acaagtggtc
     5341 ggtctcttgg tattcgctca acaaacggca aaataggagg aactattttg ccgtttgttg
     5401 agcgaatact caggtcaagg cgctgatttc acaacctctt catatacata ttagaaaata
     5461 ggtggacatg atgaataaaa gcagaataag aatactggac atcttcatgg caatcatttt
     5521 agtcgttggg atcgggatct tttcttatcc ttttgtagaa gattcactga atgattttct
     5581 tgcccagcaa atgatcatcc attatcaaaa gcaagcatcg aataagaatt cagaggaaat
     5641 aaaaaaacag caggaaaaaa tgacaaagaa aaaccaacag ttggcagagc aaaacgtatc
     5701 accagggata ggcagtttca accaagcagt tgatgcaaaa gcattaaaag atttacctag
     5761 taatgccttt tttatggaac atatgcttgg agtcatcgaa atcccaaaaa tcaatgtcag
     5821 cttacctata ttcgatcaga caacggaaat atttttgcaa aaaggaacgt cattattgga
     5881 agaaagttct tatccgactg gcgggaaaaa cacccatgcc gttctttctg ggcatcgtgg
     5941 actcccagaa gcaaaattat ttacagattt gcctaagttg aaaaaaggag accagttttt
     6001 catccagatc aatggaaaga cattggcgta tcaagtagaa aaaatccaag tggtcttacc
     6061 tgatgaagta gattctttgg gtatccaaaa aggcagagat ctcgtgactt tgcttacctg
     6121 tactccttat atggtcaata cacatcgact gcttgtgacg gggcatcgta ttccttatca
     6181 agcaaaagag gcaaaaaaag cgatccaagg aattgatcaa tggaaaaaat ggaaattttt
     6241 tatttggttt atcggtatac tacttggatc aatcggctta gtttggctac tcatcgctta
     6301 tttagattct ttggccatcg ctaagcgaaa ctatccttta tctttttatg taaagaacac
     6361 aaacggacga ccaatcgaag gaatggtctt ttctgtaaaa acgctcaacg gcaaacacta
     6421 tattactaga gaaaaagttc catttgtgaa agcttctgat gaatacggac tagtgagatt
     6481 ttctgattta aaaggcagga actatcgctt gcaacatgag gagctcctgc ttaaaatcca
     6541 tgtaaaacat aaacattcga aacagttttc aatgaaattg aaaaaaggaa gatataaact
     6601 gcgaaaagaa aaagaggtgt actatttgat tgagaaagaa tgaatgaaga tgtccaaaaa
     6661 agcagaggga acagactaaa tgaaaaaaga gagaagctga cgaaatggac gtgtaaactg
     6721 ctatttttgg tttactatct ggaacgccag cttctatttt tttatgtttc agaagaattt
     6781 tattattata gtataacttt tgaattagtt tccataacat ttggagcaag gggttaaacc
     6841 acgagcttga gcttcgctta aagtggcagg ggtatacgta ccatttccac atttatgtgt
     6901 atggtatttt gaaccggtcg gtgtgacata gacagtttgt tcattatttt gctcaggagc
     6961 tgcggcttgt gcctgctgag aggcctgttc agctgctgct tgctgtcgcg cctgttctgc
     7021 ttgttgctgt gcgattgctt cttgttgcgc ttgttctgct tgttgtcgtg caatttcttc
     7081 ttgctttttc cgttcttctt ctgcttcttt tgctttgatt gtggaatcaa ttgcagcaag
     7141 acggcttgtc aactcttgtt tgttttcagg tagcgcttgg atagctgctg aagcagttgc
     7201 atagttttct tttgtaggat ttgcttcagc ttgtgacaaa gcagattccg cttctgtcac
     7261 ttttttttct agtgcagctt tttccgcttt ttgtttctct tcttcttttt tcttttcaat
     7321 ttgttcttgt tctttcgatg aatctgccgt actttcggag atcgtggtac tgctgctgga
     7381 agcctgagct gaacttgtgc tttgagtagt agacgtagta gcaccacacc cactcaacaa
     7441 acttcctgcg accaaaaaca atgcaactaa ttttttcact aaatttcctc cttaagtaaa
     7501 aaacaaccgt tttcaataaa tgtccttttc aattgtttac ttacttgtta taataattgt
     7561 gttatgaata gtgattgagg aaacttaatc taatcccgtt ttgatacacg ggatt
//
LOCUS       DAZULG010000122         7566 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000122 DAZULG010000000
VERSION     DAZULG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.126"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1569
                     /locus_tag="ACG9CY_002428"
     CDS             <1..1569
                     /locus_tag="ACG9CY_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HFS4938907.1"
                     /translation="VHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQGIFPYRENV
                     ITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHTLPWGSKDP
                     DREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEVDPAFGDKK
                     LLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHIRFFPVDSY
                     QMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDIDGWRLDVA
                     NEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFTDSIKDYFA
                     KKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKELMKSVMAFM
                     FLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSLRKQLSKII
                     SEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILSQNCQLLED
                     GKAEIRQYGFLIMC"
     gene            1671..2930
                     /locus_tag="ACG9CY_002429"
     CDS             1671..2930
                     /locus_tag="ACG9CY_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4938908.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3077..4378
                     /locus_tag="ACG9CY_002430"
     CDS             3077..4378
                     /locus_tag="ACG9CY_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4938909.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4381..5232
                     /locus_tag="ACG9CY_002431"
     CDS             4381..5232
                     /locus_tag="ACG9CY_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS4938910.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5245..6060
                     /locus_tag="ACG9CY_002432"
     CDS             5245..6060
                     /locus_tag="ACG9CY_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS4938911.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6211..7377
                     /locus_tag="ACG9CY_002433"
     CDS             6211..7377
                     /locus_tag="ACG9CY_002433"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4938912.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2429 a         1315 c         1525 g         2297 t
ORIGIN      
        1 gttcatgatt attgggaaat tgaagtgacg agcgaaacaa gtcgcttaca gtatgcgttc
       61 catgttgttg gggaagataa cacggactgt ttttacggtg atcaaggaat ttttccttat
      121 cgagaaaatg tgattactga accaaatttt tattttcgaa taccttattt tcatcaaatc
      181 gatcgattca ctattccaga atgggtaaaa gaaacagtat ggtatcaaat ttttcccgaa
      241 agatttgcaa acggggacaa agcaaatgac cccgaacaca ctttaccttg gggaagtaaa
      301 gatccagacc gagaagactt cttcggggga gatttgcaag gtgtgaggga tcatttggat
      361 tacttgacgg accttggcgt caatggcatc tatttttgtc ccatatttaa agccacatcc
      421 aatcataaat acgatacgat tgattattat gaggtggatc ccgcttttgg cgataaaaaa
      481 cttttaaaaa acttgattga tgaagcacat aaaagaggga tacgtatcat gttcgatgct
      541 gtattcaatc atatgggtgt ccattcacca cagtggcaag acgttctcaa aaacggagaa
      601 aaatcgatct ataaagactg gttccacatc cgttttttcc cagtagattc ttatcagatg
      661 actgacttac cagaaactgc tgaaaatatc ccatatgata cttttgcttt tactccattc
      721 atgccgaaac taaatacagc aaatccagaa gtgcaaaaat atttgctgga tatcgcaact
      781 tattggataa aagaattcga tattgatgga tggcgtcttg atgttgctaa tgaagtcgat
      841 catcattttt ggaaaaaatt cagagaagca gttacagaaa tcaaaccaga catctatatc
      901 cttggagaga tttggcattc ctctcaagct tggctgcaag gggatgaatt ccatgcagtg
      961 atgaattatg cattcacaga ttcgattaaa gattattttg caaaaaagaa aatcacagct
     1021 tcacaaatgg tcagtggaat gaatcatcag caaatgcttt acagagatca ggtgaacgaa
     1081 ggaacattca acttattaga ttctcatgat actgccagga tcttgaccct ttgccaaggt
     1141 aacaaagaat tgatgaagtc tgtgatggcc tttatgtttc ttcaaaaagg gtctccttgt
     1201 atctattacg ggacagaaat agggatgact ggtgaagatg acccggattg ccgcaaatgt
     1261 atgatatggg agaaggagga acaagatcta gagctattcg gttttataaa agaacttgtt
     1321 tctctaagga aacagttgtc taagattatt tctgaaggtt ctactcagtg gctaatagta
     1381 tatgatcgtg aagataagct ctatttcaca cgggaactag agggacagat catctatgtt
     1441 tactttaatc agtcaaaaga accttgggta gtagaacagg aaaatgaggt gattcttagt
     1501 caaaattgtc agttgttaga agacgggaaa gcagagatac gacaatacgg atttttgatc
     1561 atgtgttgaa aactgttacc ggtaacaaca agaaagcgtt tgcaaatgaa gatttgtgcc
     1621 cattataata agggtgtaac agcacgttga atacacagga ggaaatgaag atgaaaaaaa
     1681 gaagtgtgaa acagttgggt ttagggttaa tctctttagg aatcttaggc ggattagcgg
     1741 cttgtggcaa tggtaactct tcagcagaag gggaagccga tgaaggaaaa acgttaacag
     1801 tttccgttga ttctggatat aaagattata taaacgaaat aaaaaacact ttcgaaaaag
     1861 aaaacgatgt gaaaatcaag ctagtagaaa aggacatgtt cgatcagttg gaatcattag
     1921 cgttagatgg gcctgctgga aagggacctg acgttatgat ggcagcatat gaccggatcg
     1981 gtgcgttagg acagcaagga catctggcgg aagtaaaact gggaaatgaa gcagcttacg
     2041 atgaaacaga caaagcccaa gtgacttatg atggaaaaat ctacggagaa cctgcagtaa
     2101 tcgagaccct tgttttgtac tataacaaag atttagtcga tacagcacca tcaacgttta
     2161 aagatttgga gaatctatcc aaagacagtc gttttgcttt tgagtcagaa gcaggaaaaa
     2221 atacaggatt tttagctaaa tggactgact tttattattc ctatggttta attgcaggtt
     2281 acggtggcta tgtatttggt gatgatggca caaatccttc agatatcggt ttgaataatg
     2341 caggtgctgt agaaggaatc tcttatgcaa cagattggtt caaaaatgtt tggccaaagg
     2401 gaatgcaaga cataaaaagt gcaggagact tcgccagcca gcaattcatg tccaataaaa
     2461 ctgcagcaat cattgacggt ccatggcaag cgcaaacata caaagaaaac aatatcaatt
     2521 atggtgtagc caaaattcct actctaaata atggacagcc ttatcaaccg tttggcggag
     2581 gaaaaggatg ggtagtcagc aattatgcta aaaacaaaga cctctctcaa aaatggcttg
     2641 attatgtaac gaatcaggaa aatcaagaaa aattctttga aatggtaaat gaaatccctg
     2701 caaaccaaca agctagggaa acagcaaagg ctaaaaacga tgaattaaca acagctgtga
     2761 ttgaacaata tgaaacagct caagcgatgc caaacatccc agaaatgggc gaagtttgga
     2821 caggtgcaga aaacttgatg ttcgatgcag catcaggttc aaaaacacca aaagaatccg
     2881 cagatgaagc agtaaaaaca atcagtgaag ccatagaaca aaaatactga ttaaaatgat
     2941 aaccgtccat ttttataaaa aatggaacag gtgcttcaga taaaagtggc aagagcgaga
     3001 tggaaaatta tcttgctctt gttgccatta tcttaaaagc agtttgcggt ttagcaagtt
     3061 tagagggggc attcagatgc agcaagataa aacaagcaat atcaacgaaa aaaaaatcac
     3121 gctttgttca cttattccag ggatggggca gtttgtaaat ggccaagctt ttaaaggtat
     3181 cgtttttttc gccatattcg tcctattcat tggtcagatg cttctgggcg gatttcatgc
     3241 attggtagga ttggttactt tgggaagtat accaatggaa gatcattcat tatttatttt
     3301 gattgaaggc actttgcaat tcatcattac aatcattttc ttgatttttt acggtttgaa
     3361 tcttttagac gcttatcgag tagcgaagat gcgtcaagag ggcaaaaaaa tcaatcggac
     3421 gatgaaggaa gtcatacata atataggtga tgaaggtttt ccttatctat tgacacttcc
     3481 cgcttatctt ctcatgattt ttacgatcat tttcccagtc ctagttacat tgttcactgc
     3541 atttactaat tatgacttca atcatattcc accagccagt ttaattgatt gggtaggggc
     3601 taaaaacttt tttagtatct tctttttaag ctcatatcgt aatacttttt ttgcagtctt
     3661 ttcgtggacc gtgatttgga ctttatgtgc aacaactttg caaatcaccc ttggtattct
     3721 cacagcggtt gttgctcacc agaaatttat taaattcaaa cgtattttcg gtatcatttt
     3781 cttattacca tgggcggtcc cagcttttat tactatcatg agtttttcga atatattcaa
     3841 cgatagcgta ggtgccatca atactcaagt gattcctttc ataaaccagc ttccttttgt
     3901 agatatcagt gcaatctctt ggaaaacgga cccgaactgg acgaaagtag caattatctt
     3961 agtgcagggc tggttaggat ttccctatat ctatgtgatg gttacaggaa tcttacaatc
     4021 gatttcagaa gacttatatg aagcagcaaa aatcgacgga gcaaatgcat ggcagcgctt
     4081 ttcaaacatc acgcttcctg ctgtcttcct agtggcagcg ccaacattta ttacacagta
     4141 tacggggaat ttcaataatt tctccatgat ctatctgttt aacaatggtg gtcccggaag
     4201 cgtaggcgga ggagctggtt ctactgatat tcttatttcc tggatctata aacttacgac
     4261 tggaacttct ccgcagtatt ctatggcttc agcaatcacg ttgattatct ccgtcatcgt
     4321 tatctgtgtg tcattgctgg tatttaaaaa gacaaatgcg ttcaatatgg gggattaaca
     4381 atgaaaaaat ttcattcgag tgctcgattc caaaaacgac tttctcactt ttttacttac
     4441 ttttttcttt taatactgtc agtcattatc atctatccgc ttttgatcac agcaagtact
     4501 gccttcaagt caggaaatat tgcagctttt agtttagatc tttcaagtga ctggacttta
     4561 ggaaacttta ctcggctatt cagtgaaaca ctttacggga cttggtataa aaatacattg
     4621 atcatttctg tcctaacaat gatcgtacaa gtgactgtag taactttggc gggctatact
     4681 tatagtcgct atcgttttat cggccgcaaa tcaagtttga tgttcttttt agtgattcag
     4741 atggtaccga ctatggcagc attgactgcc ttttatgtca tggctttctt attagaagcg
     4801 ctcgataagt actggttctt gacaatgatc tatataggcg gtggaattcc aatgaatact
     4861 tggctgatga aaggatactt tgacactgta ccaatagatt tagatgaatc tgctaaactt
     4921 gatggggcag ggcatttcag gatttttttc cagattgtat taccgcttgt caaaccgatg
     4981 ctcgccgttc aagcattatg ggcttttatg ggaccttttg gggatttcat gttagctaag
     5041 ttcctgcttc gaacacctga aaatcagact gttgctgtcg gcttacaaac atttatttct
     5101 gatgtgacaa accaaagagt ctctcttttt gctgctgggg caattttgat tgcactaccg
     5161 atttgtctat tattcttctt tttacaaaag aactttgtta ccggcctgac atctggcgga
     5221 acaaaaggat aggagcagag tatcatgcaa acatttccgt taaactattt ttccgcatta
     5281 cgtacgccaa ctcaagtgtt tgcgggacgc aaactattat catggcctaa atttttcttg
     5341 atatttgtat ttttagtttc cttgatggtc atgcctgtga ctctttttta tgccaatcag
     5401 atccaagcga ttccattaga acaatttcta tccgttcatt cgctgatcga tgaacaagga
     5461 acccaaaaat tttctgaact tgaattatct gaaactgggt tacaagcatc gcaacaaacg
     5521 atagctgtga cccctgaaat tttggtaggt gtgtctttat ctgagaaaca acaatccgat
     5581 catggaactt ttattgattt tgagaaggaa caatgggtga tccaacaaaa ggataaatct
     5641 ggtatacgcc gctatacgat gaattatagc ccatctttcc aaccagattc tgtacgtacg
     5701 cccgaagatt tccaacgatt tttagaaaga gaattttatg ccagtaatcg tccaacaatc
     5761 attttatctt atagtttaag tcttggtctt gtcctatttg tcatgacgag tctcatttta
     5821 ttcggggctt cctttttctt gtggatgaca agaaaaagcc aactttcatc cattcataca
     5881 ttcaaagaga gtgcaaattt gatgctgaat gttatgggaa taggttccat ggcagcagca
     5941 gttgtagggt ttatccactt tgactttatc ttgatgctaa gtgtccaaac aacgataact
     6001 gtcttgctgc ttttatgggt ttttgtcaaa acaagattca aagataagcg tgtcgaatga
     6061 caagtttaag cgatttatgc gacaatgaat gtgaaaaaaa tgaaaaataa agtagttttt
     6121 gaaaaatttc tgaaaaaatt aaacttctta gttgattctt ttctttttta gtattaacgt
     6181 aatagggtag tttttttaag gaggaaaatc atgttagaga aaactttata caatcaatta
     6241 ttttcacgtt ctttctcgct ccctgtcgaa gtaaattatt gggacggtac aacgaaacaa
     6301 tacggagata cggacactcc tccccaaatc aaaatcaata tccatgaaga aatcccaatg
     6361 aaagagatta caagtaacgc ttctttggca ctaggagaag cctatatgga tcatcgaatc
     6421 gagatcgagg gaagtatcca agcattgatc aacgatgctt atcagcaagc aaacagcttt
     6481 ttacgaggaa aagattatct aaaatggctg ccaaagaaag aaaaacatac gaaacaaaaa
     6541 aacaaagaag atatccatgc gcattatgac ttaggtaacg acttttataa actttggcta
     6601 gatccaactt tgacttattc ttgtgcttat ttcaatacac ctgaagatac gttggaacaa
     6661 gcacaaatca ataaagtcca tcatatctta gacaaactct ttatcaaaga aggcgaaacg
     6721 ttgcttgata tcggttgtgg ctggggaaca ttgatgttca ctgctaccaa agagtataat
     6781 gtcaaggcaa ctgggatcac actaagtgaa gaacaatacg atttgattac caaaagaata
     6841 aaagaagaac atttagaaga taaatgccgt gtattattaa tggattaccg cgaactaaaa
     6901 ggcgaaacgt ttgatcatat cacaagtgta ggtatgttcg aacatgtagg ttctgaaaat
     6961 cttgaaggtt atttcaaagt ggtaaaagat ttattgaaac caaaaggaac agctttgatc
     7021 catggaatca gccgacaaca aggcggtgcg acaaatgctt ggatcaataa atatattttc
     7081 cctggcggtt atgtccctgg tgttactgaa ttagttgata atattacaaa aaacgatttg
     7141 caactgattg atttggaaag cttacgtcgt gactaccaat tgacattaga acattggaca
     7201 aagaattttc atcaagtcca agatcaagtc acgaaagaaa tgggtgattc cttctatcga
     7261 atgtgggatc tatatttaca agcttgtgca gcttcatttg aatcaagtaa tatcgatgtg
     7321 attcaatatt tgcttgttca acctggaaac aatgacatcc caatgcatcg tgcataattg
     7381 aggcaaaagt actgtttaaa gactatttaa aaagaagcta agttttatga tgctccagaa
     7441 gtgaactttt ttgtctaact tctaggattg tctctaaagc ttagtttctt tttctttata
     7501 ttttcataat aaaagtttgg ttctttaatt tttaaacatg acagaaaatt ttttcttcgt
     7561 tatact
//
LOCUS       DAZULG010000123         7238 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000123 DAZULG010000000
VERSION     DAZULG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.127"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..728)
                     /locus_tag="ACG9CY_002434"
     CDS             complement(<1..728)
                     /locus_tag="ACG9CY_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4938913.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLS"
     gene            complement(848..1495)
                     /locus_tag="ACG9CY_002435"
     CDS             complement(848..1495)
                     /locus_tag="ACG9CY_002435"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFS4938914.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1579..3006)
                     /gene="uxaC"
                     /locus_tag="ACG9CY_002436"
     CDS             complement(1579..3006)
                     /gene="uxaC"
                     /locus_tag="ACG9CY_002436"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4938915.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3080..4159)
                     /gene="uxuA"
                     /locus_tag="ACG9CY_002437"
     CDS             complement(3080..4159)
                     /gene="uxuA"
                     /locus_tag="ACG9CY_002437"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4938916.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPACEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4162..5793)
                     /locus_tag="ACG9CY_002438"
     CDS             complement(4162..5793)
                     /locus_tag="ACG9CY_002438"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4938917.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6097..6768
                     /locus_tag="ACG9CY_002439"
     CDS             6097..6768
                     /locus_tag="ACG9CY_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4938918.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(6928..7119)
                     /locus_tag="ACG9CY_002440"
                     /pseudo
     CDS             complement(6928..7119)
                     /locus_tag="ACG9CY_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         2328 a         1428 c          985 g         2497 t
ORIGIN      
        1 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
       61 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
      121 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
      181 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
      241 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
      301 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
      361 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
      421 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
      481 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
      541 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
      601 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
      661 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
      721 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
      781 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
      841 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
      901 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
      961 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     1021 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     1081 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
     1141 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
     1201 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
     1261 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
     1321 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
     1381 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
     1441 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
     1501 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
     1561 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
     1621 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
     1681 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
     1741 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
     1801 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
     1861 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
     1921 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
     1981 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
     2041 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
     2101 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
     2161 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
     2221 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
     2281 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
     2341 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
     2401 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
     2461 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
     2521 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
     2581 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
     2641 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
     2701 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
     2761 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
     2821 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
     2881 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
     2941 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
     3001 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
     3061 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
     3121 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
     3181 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
     3241 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
     3301 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
     3361 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
     3421 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
     3481 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
     3541 ggtgaactgc cattttaatc ccaacttctt cacatgccgg gataattgct ttgagaaaat
     3601 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
     3661 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
     3721 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
     3781 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
     3841 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
     3901 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
     3961 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
     4021 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
     4081 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
     4141 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
     4201 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
     4261 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
     4321 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
     4381 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
     4441 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
     4501 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
     4561 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
     4621 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
     4681 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
     4741 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
     4801 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
     4861 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
     4921 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
     4981 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
     5041 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
     5101 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
     5161 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
     5221 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
     5281 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
     5341 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
     5401 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
     5461 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
     5521 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
     5581 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
     5641 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
     5701 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
     5761 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
     5821 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
     5881 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
     5941 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
     6001 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
     6061 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
     6121 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
     6181 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
     6241 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
     6301 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
     6361 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
     6421 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
     6481 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
     6541 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
     6601 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
     6661 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
     6721 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactctt
     6781 tttaaattaa atggttgttc tgatcagaaa aatatcgctt gttttcttat tatgtaatat
     6841 atttcataaa ctattctgca taacaactat tgtttgtact ttttacattg gataactaat
     6901 tatgactgct gataactttt gatattaaac tagtatctcc aatacaaata aaaaatagat
     6961 atcttgttca tcaaaattag ttcgtggata cttaccttca tttacatcat tcagcggttt
     7021 cccattttct tttattctat tatttcattt tctaatagac ttggcttcat ttcgcaatac
     7081 tttttttcaa taggcatcaa attggtgttg tttagatatt tagttgtagt tttgtgacat
     7141 gataagtatg ctcctttttc tcttttccac taatacaacg atcaaagggt gcttttttca
     7201 aaaaactaga aaaatcaaaa taagattcgt attttgat
//
LOCUS       DAZULG010000124         7201 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000124 DAZULG010000000
VERSION     DAZULG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.128"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(219..1565)
                     /gene="gor"
                     /locus_tag="ACG9CY_002441"
     CDS             complement(219..1565)
                     /gene="gor"
                     /locus_tag="ACG9CY_002441"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS4938919.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1699..2952)
                     /locus_tag="ACG9CY_002442"
     CDS             complement(1699..2952)
                     /locus_tag="ACG9CY_002442"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS4938920.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(2958..3881)
                     /locus_tag="ACG9CY_002443"
     CDS             complement(2958..3881)
                     /locus_tag="ACG9CY_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS4938921.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4102..6594)
                     /locus_tag="ACG9CY_002444"
     CDS             complement(4102..6594)
                     /locus_tag="ACG9CY_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS4938922.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6601..7068)
                     /locus_tag="ACG9CY_002445"
     CDS             complement(6601..7068)
                     /locus_tag="ACG9CY_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS4938923.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         1979 a         1578 c         1161 g         2483 t
ORIGIN      
        1 ttttgaactt aagagtgcat accaaatgga ttcatttact cttcgtcagt tacgaagata
       61 atcaaaatct ccaacttccc tacttatttc gttttgagaa ttatcgaagc aatcttacat
      121 aaataactaa aaaacaaaaa accagaaaat cagtatactg attttctggt tttacatggt
      181 ttgttcaata aaaagctgtt ggaactgtta atattaaatt atcgcatcgt tacgaattct
      241 tccgctcctg ttggatggat ggctactgta ttgtcaaagt cttctttagt tgcacccatc
      301 ttgatagcca cggcaaaacc ttgcagcatc tcatcgacac caacaccaat agcatgtaag
      361 ccgacgattc tttcttcttt tccgacacag atcaatttca tttcgcattt ttggcgataa
      421 tcatttaatg caaagtacat tggggtaaag cgtgaacgat aaactttcaa ttcattttcg
      481 ccatattttt ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg tggatgcgta
      541 aagacgactg tcggaacaag tttatagtca agataagaat ctttctttcc attaaataaa
      601 cgttctgata aacgtctacc agctgcgatt gcaacaggag tcagatctaa tttaccgatc
      661 acatcaccaa cagcaaaaat atgttcatcc gttgtatttt ggtatttatc tactttgata
      721 aacccttttt tgtccaattc cacatttgta ttttccagac ctaatttttc ggtattcggt
      781 acacgtccac cggcaaaaag tacacaatca ccagttaagg tttcaccatt ttcaaatagc
      841 accgtatatt cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc gtaagttttt
      901 attccagctt cttggtacat ttggatcaaa ttgtccgaaa gcattttatc aaacgttcta
      961 agtggtcgtt cttttcgata tgcccacatg acttcactac ctaaaccacg aaatacacct
     1021 gaaagttcag ccgcaatgta tccagcacct aaaacgattg ctctcttagg taactctgtt
     1081 aattcaaaaa atccatttga atcaattgcg tattctccac caggaatggt cattttactt
     1141 gcacggcctc cagtagcgat caagatttta ggagcacgat aggtttcacc gtttacttcg
     1201 atcgtttgac tatcgataaa agtagcgtaa ccttttatag cctcgacatg attgctatct
     1261 aatcctcttt gataagcacc gtgtagaaaa tcaatatatt tttctcgtct ttctactaga
     1321 ccggcgaaat caacattttt taaatctgct tggataccgt aacttgaggc atctcgttca
     1381 attgtttcca aaatcgtact tgcttgccac ataacttttt taggtacaca accgacgttg
     1441 acacatgttc cgcccaattc attcgcttca atcagcaaaa cttttgcgcc atgcatccct
     1501 gctctgtttg ctgaagcaat accgccagag ccgccaccaa ttacgatgta atcatatatt
     1561 tgcattttgt tcctccttat tgaagagatc aatcaaaacc tttatttaac gatccttttt
     1621 ctacagaaaa caagtgtaac acttttcttg aattttgtat gcgaagatgc ttaaaacagg
     1681 gatatatcag ctttttcctt atttacgttt tcggatcata tctccaggtt tcaccggctg
     1741 tgcgactggc atagttaaaa tcatcattgg atttggtgct cggtcaatgg attcgccttc
     1801 ttcattatgc ataacttcaa cggtttgact gaaatgactg aatcctggac cataaaattc
     1861 gatttcatct cctacagaaa acaagtttct ctgacgtatc gtagcaatct gtgttgcctc
     1921 atcataagat aatacttcac cgacaaattt atatactggg attttacgac gttcaccgaa
     1981 taattgttcg ttttcagttg gtgtattgta gtaaaaacct gtagctaatt ctcgttgtgc
     2041 tactttccat aactcgtcga tccattcttg cttgcagaca tagttttcag gatcttccat
     2101 ataactgtct acagccgctt tatagacatt tgacaccgta gatacataat ggatcgattt
     2161 cattcttcct tcgatcttga agctgtctac gccgttttca atcaattctg gaatatgttc
     2221 gatcattgac atatccactg cactcatcga gaattcttct tccacttcgc ctgtatcagt
     2281 taaactgttt cgttcttgac caaaaggcat atcgaacaat ccatacttcc agcgacaaga
     2341 ttgagaacat ccgccacggt ttgcatcccg catcgacata tggttggaca atgtgcatcg
     2401 accagaataa gagatacaca tggcaccatg gataaatgct tcgatttcgg tatccgtatt
     2461 tttacgaatc tctgccactt cctccattga aacttcacga gctaaaacga cacgttccaa
     2521 cccttcttct ttccagaatt ccaatgtctc aaaattagtt gctgaagctt gagtggataa
     2581 atggatcggc agacctgggg cttctgtcgc acaaatttcg atcaaagctg gatcagaaac
     2641 aatcactgca gaaataccga catcacgcaa actgcggaaa aattcccctg ctccatcttc
     2701 atttccttca tgggtgacca tattagcagc aacgtatacc ttcgcatcat gttcttttgc
     2761 aaaagcaact ccttcagcca tttcttcata agtaaaatta cctgcacggc ttcttaaacc
     2821 atatgcattc ccgccaatat atactgcatc tgctccataa tggatcgcgg tttttaactt
     2881 ttccagtgtt cctgctggcg ctaggatttc tggtcgtttc aattttcttt ttactgtcat
     2941 ttctttcatc atactcctta tttgatagca gaaggatcaa tataaaagaa tcctgtatct
     3001 aattcacggt tttcaggatg caatttactg atttcgctag tcattttttc cgctttttct
     3061 tgatcccagc taccttctaa aatttgtttt tttgcttcgg cgaatagtcg agtgatgttt
     3121 acgaattttt cgcctggagt aaataatccg tccaatttcc atgtacagta atgatgctga
     3181 taaagttcat tcaattcatt cgagagattc agatcattgc tggcaaaaat atgtgtgcca
     3241 tgactatctt cataaataga ataatgtgtc tcttcttttt taggttccga gataaaaaga
     3301 cctctttcac gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt ctgtagcagt
     3361 ggacgtttgg attgatggat gcatgtagca ccatacacaa gggtttctac aggaatatcg
     3421 agtttttctt ccatcgcctt catctcttca aaaggaactt cacgagcaag tactgctcca
     3481 actgcgcctt ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa tgtttcagca
     3541 tcataaatat acggtatacg caattcagga ttttttttca tcgtataaat aatacctgta
     3601 tcaccaactg tgatcacatc tacgtttatt tcttttaaaa aagccaaata ttcaggtaca
     3661 tttttcattt tttcaggatg aatgatccca tttacagcaa tattcgcttt tttaccatac
     3721 ttgtgtgcta attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc aggcagacgc
     3781 aatccaaatt gctcttcacc gaaatatagt gtatctgcac ctgcagcaag caactgttct
     3841 gcttgttcaa tagtttctac tgtggttatg agttcgatca taattttctc ctcctacaac
     3901 ttcataatag tagcatagct ctaaaaactt tccaatactt cttctattta aagtcggatt
     3961 tttgaaaatt atttcacaaa aaatcaaaat aaagttcgat atattctgtc caaatataaa
     4021 aagagattgt gacaaaaatc ttgtcacaac ctcttaatca agtcttacta ttataaaaat
     4081 tcagctacac aggccaagta actataggga acctactgtt tcctttttag gtccgcgaac
     4141 attcaatgtg atcttacctt ttgaagcacc tactgttacg tggtcaccta aacgaatttc
     4201 tccgccaagc aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg cgcgacggat
     4261 tggacgtgca ccatattcag gatcaaatcc agctttcccg atcacatcga ttgcagatgg
     4321 tgtgattttc acttgaatat cttgttcttt caatcgtttg ataatcgctt tgctcatgat
     4381 ttggacgatt gtatggattt catctttact caatgaatgg aagacgacag tctcatcgat
     4441 tcgattcaag aattcaggtc ggaaagcttt ctttaactct tctaagatac gtttttgcat
     4501 ggctttgtga tctttagtaa catcttggac attgaatccg acattttttt cttctcgaat
     4561 ttgtgtcgct ccgatatttg aagtcataat catgattgta tttctaaagt ccacttttct
     4621 acctttagaa tctgttaaat gaccatcatc caatacttgc agaagtaaat tgaaaacgtc
     4681 tggatgtgct ttctccactt catctaagag aatcactgaa tatggttttt gacgaatttt
     4741 ttctgtcaac tgtccacctt catcataccc tacgtatcct ggaggagacc cgattaatcg
     4801 gcttgtactg tatttttcca taaattctga catatcaaca cggatcaggg catcttcact
     4861 tccaaacatg acttctgaaa gtgctttggc caattctgtt tttccgacac cagttggtcc
     4921 aaggaacatg aatgaaccaa ttggacgatc aggatctttc aatccgcttc gtgcacggcg
     4981 gatcgcacgt gaaactgctt tgacagcctc atcttgacca acgacacgtt catgcagaag
     5041 tccttccagt tctaacaagc gttcgctttc ttttttctcc aactgctgta gagggacacc
     5101 agtccactcc gatacaactg tagcaacatc ttcttctgtt acacgatcag aataaccttg
     5161 ctcctgtttt gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg ctttttcttg
     5221 tctgcgtaat tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt ccttttcttg
     5281 tactagttca tcgatttcta gtttgatcac agttgatttt gttaagtgat cggtttgatc
     5341 caaacggact ttcgctgctg attcatcgat caaatcaatt gctttatcgg gtagttgacg
     5401 agaagtgata taacgaacag aaagattgac tgctgctcgt acagcttcat ctgtgatctc
     5461 cactccatgg tgctcttcat atcttgtacg taatccttga agaatcactt ctgcttcttc
     5521 aggagttggc tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag aatctttttc
     5581 gatatatttt tgatattcat ctagggtcgt tgctccgatc gtttgtaatt ctcctcgagc
     5641 taacgccggt ttcaagatat tcgaagcatc aattgctcct tctgctccac ctgcaccaat
     5701 taaggtatgc aattcatcaa tgaataagat tacttgacca tcattataga tttcgtcgat
     5761 gacctttttc attcgatctt caaattcacc gcgatatttc gtaccagcaa ccaaagatcc
     5821 catatcaagc atcatcaggc gcttttcttg catgtcttct ggcacttcac ctaaaatgat
     5881 tttttgagct aacccttcag caatggctgt tttgcctaca ccaggttcac ccaccagtac
     5941 agggttattc tttgtccgtc ggctaagaat ctggataagc cgtttcactt ctctcgaacg
     6001 accaacaact gggtccagct tgttttcttt tgctaatttc gtcaaatcac gtgctaatga
     6061 atctagcgta ggtgttcctt gcggtgtttg ccgtttatct tgtacattta catttctacg
     6121 acgattcatt ccttttccag cagaatcacc agctagcccc attttttgtt tcagaacttg
     6181 acgggctttt gaaaggctga tacctaagtt gatcatgatt cgagtagcca tgatactttc
     6241 atcttttaat aagctcatca aaatatgttc tgttccaact ttttgagaac cagttttctt
     6301 cacttcgctg ctagctaaag caaaaacttg tttcattctt ggtgaatatg gaagaaagac
     6361 tcctttaggg taagaacgga taccaccata accaatcaga tgttcaattt cttcataaat
     6421 ttccgtttca ttcaaatcca tttcacgtaa tacttttcca gcaattccat tttgctcgac
     6481 cactaaggct agtaaaacat gttctgaact aactgattga tgtttcaacc gttttgcttc
     6541 ttcttgtgca atttctaata ctgcttgggc tctttcagta aaaagttctt ccatctaatc
     6601 tcacttccca tcttcataac ttaaacgttc cagaaatgct cggagaatcc gagcacgtgt
     6661 atgatcttca tattcattga aattcaatgt gttttttgca attgcactca acatcaagtt
     6721 tccttctttt tttgtaatta tttggtcttc ataaagtttt tgaataatag caaaagcatt
     6781 tttctcactg attgtatcat caaataactg atttatctgc tcaagtaatt gttttttatc
     6841 tgaaatcctt acttttgcaa ttcgtatata accaccacca ccacgtttgc tttctactag
     6901 ataaccacgt tggatggtaa aacgggtatt aatcacatag ttgatttgtg aaggaacaca
     6961 gttaaagagg tctgccattt caatccttcg aatctcgatc atttcactat tttccagaat
     7021 tttctttata tatgattcaa ttaaatccga ggtattctga tgtgccatct tattcattcc
     7081 ctttgactaa tattgacctt aattataacg aatttgataa tagaaagaaa gaagaacgct
     7141 ttttgttaaa taaatccttt agaatcaaca aaaaaaaata cggaatatac tattccgtat
     7201 c
//
LOCUS       DAZULG010000125         6987 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000125 DAZULG010000000
VERSION     DAZULG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.129"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            336..692
                     /locus_tag="ACG9CY_002446"
     CDS             336..692
                     /locus_tag="ACG9CY_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4938924.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            702..1601
                     /locus_tag="ACG9CY_002447"
     CDS             702..1601
                     /locus_tag="ACG9CY_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4938925.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2121..2849
                     /locus_tag="ACG9CY_002448"
     CDS             2121..2849
                     /locus_tag="ACG9CY_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4938926.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2994..5021
                     /locus_tag="ACG9CY_002449"
     CDS             2994..5021
                     /locus_tag="ACG9CY_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS4938927.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            5129..6226
                     /locus_tag="ACG9CY_002450"
     CDS             5129..6226
                     /locus_tag="ACG9CY_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4938928.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6533..>6987
                     /locus_tag="ACG9CY_002451"
     CDS             6533..>6987
                     /locus_tag="ACG9CY_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938929.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTRV"
BASE COUNT         2452 a         1116 c         1411 g         2008 t
ORIGIN      
        1 tcttttattt gataagttta ctctaccgaa gagttgttaa taatccgtga acaaaaagta
       61 attctttttc caatagttgt taaaggtttt ttatttttta tagtattttt tgttatttaa
      121 gaaaggaata aggttaaaca aataacaggt tgctttttgg gtctttttcc gttcaagaaa
      181 ctgaacgaag aaaagggaaa gttgtctctt ttgtgataag agaaatcata ataaacgaat
      241 gacttacttt ccaataaatt gagaacagtc agaaccagac aaattagttg catctgatga
      301 ataaagtgat tattctatga catgaggtga tacagatgaa attagaagtg acaaaagaag
      361 cacaagaagt attgaagaat aaattagaag cagacgatca acttttatta gatatcgaaa
      421 acggtgacgg tccatttgca gaaagccaag tcagttgcca attggatacg gcatttcgat
      481 tgatcattgt caaaaaagat acacaaacag atctaagtct ttattctgta gtggctgata
      541 caccagttgg accaatcaaa atcaaagaca gtgcaatact ttacatggat gatccatcca
      601 cacttgcttt agaaccaact tataatagtt tacaattaaa aggaccaagc ggcttgagaa
      661 aaggcaactt gcaagttgtt cgaaaagact agagggataa aatgggaaaa ttggcgatca
      721 tcagcgacct acatgtggac atcaataaat tcggcgaatc agagctgatg cagctttggc
      781 aagttttgca aaagcacaag atcactcggc ttcatctagc cggagatacg gctaataaag
      841 tagaccgttg cctagctgtt gtcgaatttt tcgtcaataa aggattatct acgacatttc
      901 atttcggcaa tcacgagttg gcagatgtgc ccaatgaaca gatgatggac cattttccag
      961 acccacattt tttaaatgaa cggtatattg ctttaaatga gcagaccgtt cttttaggga
     1021 tgaatggctg gtacgactat cagttttccg aattgaaaga ttacgaggca atcgtacgaa
     1081 tgaagcgttt gtattggtat gatcggttca tccagcgtgg gaaaacagat ctcgaggtca
     1141 atgcagaaat gcttgcagct gcaaaaaatt tgctagacca gctggaagca aaaaaacttg
     1201 aggtgatttt atccactcat ttcgttccta aacgggcatt tatcatgtat caatctgctc
     1261 cttatgagcg ttggaacaaa ttaaatgcat ttttaggatc agaatcattc ggagaattac
     1321 ttgaccgtta ttcaaatatc aaacaagtag tttttggcca tacgcatcgt cgctttgaag
     1381 ataaatccat cggtcacacg atttacagct gcagaccttt tggttattat tatgaatggc
     1441 agctgacccg agattttgtc ttgaaagaac agttgctaga cagctatgat cctatgaaat
     1501 tacgtgtcct cttgcgtgcc catcacccag cttttcaggc ctatcaaaca caacatttgc
     1561 aagaggaatt tgaacaagcg atgacaatcg ttccgtattg agacagcatt agtaatgtgt
     1621 aaaagaaaat accttgtaaa acataaggat aagtaagagc agtagatcca tttatcgtgg
     1681 atatgctgct ttttttgcgg aaaataatca gcgaatgttt atttgatcgg atgatttgta
     1741 actcgcagag tactagatgc aagcgtgtat tctttttttg aacgataatc acaaaaaaaa
     1801 atataatata cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag
     1861 tcacattttg atagatagga gggggactta cgaattattt taaaggctat gacagctgta
     1921 tttctagatt gtgtttgcat caagttgcaa taaaaactag aaatcaaatc gggctgataa
     1981 tcatttataa ctaatcgata cataggacca aatataggtc tatgttcttt tatttcataa
     2041 gtatcgtaaa acagaatttt gctatggatt aatccaatcg actgatatgt ttagtaaata
     2101 aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt aacagcagca atgatgttat
     2161 tcagcatggt agcaagtacg acttttgttt ctgcaactga agtataccca aaggaatata
     2221 acacagaagg aacgattaca tttgaagcag gagatgaggg tgtaactcct cctgttgatc
     2281 cagagaatcc tgatcccaat aaacctgtcg atccatcaga tcctccaagc cctggaacag
     2341 gaggggcatt atcgattgat tatggatcaa aattcaaatt tgggactcaa aaaatttcta
     2401 ctgcggataa aacgtattat gcagctgctg atgtgatgaa cgatgggtct agaaaaccga
     2461 catatgtcca agtgacagat aggagaagta ccttatctgg gtggaaatta agtgtctctc
     2521 aaccagaaca gtttaaaact gccagtgggg atgaactagt tggagcacag ctgaaattta
     2581 cgaaagggca ggctgtttct ttggttgatc caacatatac gccgcaaacg gtgaattctg
     2641 aattaacgtt gacaccggga ggaaacaata cgttagcaat taatgctaaa tcaggaacag
     2701 gtgtggggac ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac
     2761 agctttcggt acccggtaaa tctgtcaagt tagcgcaaca atattccact aaattagttt
     2821 ggacattaga agatacacct aacaactagt ttactgaaaa gaactgttaa aaataaatac
     2881 gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt caaaggctag gctaaatttg
     2941 tttggatacg aatagttcat ctaggaattt aacgatcagg aggaaaagat atattgaaaa
     3001 agaggaggat cgtgctcatt ttaagcagtc tcttagtgac taatatcttt ccgatcatag
     3061 ggtactcttt ggaattgaat cagttggagc atggaaatag aagagacgaa gaaagcaaac
     3121 tcacttcgac attcgacagt ttaatagaat ccaaccagac acaagaaagc agtgaggatt
     3181 taagtatttc gagtagtgga gagaccactg acaacgtaga agctgagggt tcggacaaac
     3241 aaggactaga agatgcacca atttctgcgg ttgagctgcc tgacgatccc aatattattt
     3301 ctatcgataa aatttttcaa acacctatcg gtactagcac gtccatattg gaaaatggga
     3361 agttactgca gttgaaccca gcagaaaggt ctcaaagagg agctatttgg tcaaataaga
     3421 agattagttt actctcggat tttacattta aatcttattt atacttagga aatcaacgag
     3481 atagagcggg agatggcatg acctttacat tgactggcga tcctagaatg gctagtaatc
     3541 ccagtcaagt tattggatcg ccaggtatgg gaattggctc atattctact cgttctggcc
     3601 aaccttacat tgcgaatgcg ctctcgattg agtttgatac gtataaaaat agtggttctt
     3661 ctgatcgaat ggatcgagag ataactgctg ataatagagg gcatggacat gtcgcttttg
     3721 tcacgcctaa accaaataat aataattatt ctggagagca tactggcgtg acggtagctc
     3781 ctacatttct ttctaatggc acatggcgct tgttaataat taaatgggac gcggagcaaa
     3841 gaagtcttac ttatgatttg gaaggtgtcg gtcgctcttc tcggattata cctgatctca
     3901 ttaggcagtt tggttctaca gaagtaaatt gggggtttac ttcttcaaca ggtacttttt
     3961 ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca acagctgatg
     4021 taaaagtcaa cgatagtgat tatggggcca tcgctgaagc tggaggaaac gataagataa
     4081 ctatcagaaa tacattgaag gtcgaggggg attttcttga atataagaaa atgcagacta
     4141 gtatcgacct cccacaagaa ttaactattc ccgagcatat cttgatcaat ggaaatcaaa
     4201 ttgatgccaa agatatcttg ataaccggaa atcgagtcac gctaaatctg acagattatt
     4261 ttattgctgg agatacgtta ataatcgaat tggagacaac tttgcaggaa gtaattcctg
     4321 aaaaagtact cactatgcat tttgaatacc ttgaaaacgg gaatttgatt gagcaatcga
     4381 atgaaataaa cctaaagatc gcaaagctga aggagataga tatccatgtt tattataaag
     4441 ataaagacag tcagattgat attgctgaaa tgaaaacaat cactgggaaa ataggagaga
     4501 agtataaaga aactcctatt gatattgaag ggtatgtttt tgactcggat tcagcgaata
     4561 cagaaggcat cttctccgaa aatacagagg atatctattt ttattacaga aaaggcaaac
     4621 tttatctctc aactgctcct caatatgtca attttggaaa acataaaata tccagtagac
     4681 cattaagtga attcggacag ttttcaggtg ggttgaaaat tatagatgaa cgggcttttg
     4741 gaagttggaa attacaattg aaacaagaga aagttcttac aaatggagat tttgagcttc
     4801 cagaagctct ttcctttgtt acctctgaaa atacaacggt tatagggaca tccgctgtta
     4861 cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta gattctacag
     4921 ctcatcgtgg aatcagaata aatattcctg tagaaaacca aagaatcgga acctttgagg
     4981 ggaaactatc ttggatactt gcagatgtcc caggaaatta acaggaagag aagaatataa
     5041 caaaattatt gagtttcaag aagggcacag gtaccatagg ctcttgtgtc ccacagagac
     5101 aagattttgt tatagtcttc tcttacatat gattaaagga gcgattttct tgagaccaaa
     5161 acaacaatta tttataacac tcctagttat tttaggggca ctttttaaca atatatcagt
     5221 gtcagctagt gcatttaatt tttcagtgac gccgataact tcagagaatc aaatcgacaa
     5281 gagaaaaact tacttcgatc ttcaacttga tccggatcag gaagtagaag taaaagcgga
     5341 attgagaaat gatacagaaa aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac
     5401 caattcaaat gtgatggttg aatacggaaa aaacgagatt gaaaaagaca tgagcctgat
     5461 ctttgatctg gtagattatg tgagctatcc acagatagtg acgctaaaac ctaaaagtgt
     5521 ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga tttgatggtg tattggctgg
     5581 tgggatcaca ttccaagaaa tccagacgga aaaggaccaa acagaaacta aggatcaaag
     5641 cttatcgatt gagaatgaat acacctatat tattgctttg ttgatgcaac agacactgaa
     5701 cgaagttgct ccgaacctta ctcttcatga agttaaacca gatcaaatca atgcaagaaa
     5761 tgtcatctta gcaaacgttc aaaatgatca aaaaacctat atcaatcaag tagttattga
     5821 aacaaaaatt acaaaaaaag gacattctga agttttatat caagaggaaa aagaaggatt
     5881 gcagattgcg ccgaatacaa atttttcttt tccaactgct ttaaatggtc aaccgctaac
     5941 cccaggtgaa tatcacctta caatgactgt tttaggaaat gaaaatgaaa atggaaaatt
     6001 ttcgagaaaa aaaggaaata ctacgatcaa ctataccaat caatgggtat ttgaaaaaga
     6061 ttttacaata gacggaaaag tagccaagga attgaacacg acggatgtta cgattaaaaa
     6121 agacaataat tggatttata tcttagtagc ttcacttttc ttattgttta ttttattatg
     6181 catacttttg tttgttttga gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa
     6241 atagtaaatt tttattattg taggatggtt gggctctttt tataacaata taaaatatac
     6301 cattttatta ataaaaatgc atactagtaa atgatataat tctaataaca ggtttgatcg
     6361 cgagaaaggc caaataaaac gctctccaaa atgttaaaaa aaagacagga cgatgtatat
     6421 tatacaaaca agaatcgctg acaacagtga ctcggatcca ggaaatcagt tttatcccca
     6481 gctagttggt gaatgcatgg aaagtaatat acgtagaaag agctgatgac taatgaatta
     6541 tgagtttttt ttaagcaaag aagatcacag aagttattca ttacttaaat atttagaagc
     6601 aagctcaaat ttatcagagt caatatcgaa tgttcaagaa gagttgtcgc tatcgacatt
     6661 tctcttgaaa aaaacgatcg ataagctgaa acatgattta caaaagttcg atctagagaa
     6721 aaatttgcga gtaaccgtca gtgaattgga tatatgtctg gaaattaatg gaaaatgttc
     6781 tagcaaagct ctgttatctc gttatataac ggattcttta tctatgaaaa tggtattggc
     6841 tttctttcag gaaaaatata tctcagtaga aaattttgct aaacagcatc acgtgagtta
     6901 ttctgtggct tataaagtat tacaaagact aaaaagaaat ctaaaaaaat atcaaatttt
     6961 ttttgaaaag agaaagttga cgagagt
//
LOCUS       DAZULG010000126         6869 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000126 DAZULG010000000
VERSION     DAZULG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.130"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(195..653)
                     /locus_tag="ACG9CY_002452"
     CDS             complement(195..653)
                     /locus_tag="ACG9CY_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4938930.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(666..1997)
                     /locus_tag="ACG9CY_002453"
     CDS             complement(666..1997)
                     /locus_tag="ACG9CY_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS4938931.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(2008..3450)
                     /locus_tag="ACG9CY_002454"
     CDS             complement(2008..3450)
                     /locus_tag="ACG9CY_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4938932.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3437..4201)
                     /locus_tag="ACG9CY_002455"
     CDS             complement(3437..4201)
                     /locus_tag="ACG9CY_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS4938933.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4305..5180)
                     /locus_tag="ACG9CY_002456"
     CDS             complement(4305..5180)
                     /locus_tag="ACG9CY_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4938934.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5435..5519)
                     /locus_tag="ACG9CY_002457"
     tRNA            complement(5435..5519)
                     /locus_tag="ACG9CY_002457"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5483..5485),aa:Leu,seq:caa)
     gene            complement(5537..5608)
                     /locus_tag="ACG9CY_002458"
     tRNA            complement(5537..5608)
                     /locus_tag="ACG9CY_002458"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5574..5576),aa:Gln,seq:ttg)
     gene            complement(5618..5690)
                     /locus_tag="ACG9CY_002459"
     tRNA            complement(5618..5690)
                     /locus_tag="ACG9CY_002459"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5655..5657),aa:His,seq:gtg)
     gene            complement(5752..5836)
                     /locus_tag="ACG9CY_002460"
     tRNA            complement(5752..5836)
                     /locus_tag="ACG9CY_002460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5800..5802),aa:Leu,seq:caa)
     gene            complement(5857..5927)
                     /locus_tag="ACG9CY_002461"
     tRNA            complement(5857..5927)
                     /locus_tag="ACG9CY_002461"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5893..5895),aa:Cys,seq:gca)
     gene            complement(5973..6044)
                     /locus_tag="ACG9CY_002462"
     tRNA            complement(5973..6044)
                     /locus_tag="ACG9CY_002462"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6010..6012),aa:Gln,seq:ttg)
     gene            complement(6056..6128)
                     /locus_tag="ACG9CY_002463"
     tRNA            complement(6056..6128)
                     /locus_tag="ACG9CY_002463"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6093..6095),aa:His,seq:gtg)
     gene            complement(6131..6204)
                     /locus_tag="ACG9CY_002464"
     tRNA            complement(6131..6204)
                     /locus_tag="ACG9CY_002464"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6170..6172),aa:Trp,seq:cca)
     gene            complement(6211..6291)
                     /locus_tag="ACG9CY_002465"
     tRNA            complement(6211..6291)
                     /locus_tag="ACG9CY_002465"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6255..6257),aa:Tyr,seq:gta)
     gene            complement(6293..6368)
                     /locus_tag="ACG9CY_002466"
     tRNA            complement(6293..6368)
                     /locus_tag="ACG9CY_002466"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6333..6335),aa:Phe,seq:gaa)
     gene            complement(6404..6476)
                     /locus_tag="ACG9CY_002467"
     tRNA            complement(6404..6476)
                     /locus_tag="ACG9CY_002467"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6441..6443),aa:Asp,seq:gtc)
     gene            complement(6486..6558)
                     /locus_tag="ACG9CY_002468"
     tRNA            complement(6486..6558)
                     /locus_tag="ACG9CY_002468"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6523..6525),aa:Val,seq:tac)
     gene            complement(6574..6645)
                     /locus_tag="ACG9CY_002469"
     tRNA            complement(6574..6645)
                     /locus_tag="ACG9CY_002469"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6610..6612),aa:Glu,seq:ttc)
     gene            complement(6660..6749)
                     /locus_tag="ACG9CY_002470"
     tRNA            complement(6660..6749)
                     /locus_tag="ACG9CY_002470"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6713..6715),aa:Ser,seq:gga)
     gene            complement(6789..6862)
                     /locus_tag="ACG9CY_002471"
     tRNA            complement(6789..6862)
                     /locus_tag="ACG9CY_002471"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6826..6828),aa:Asn,seq:gtt)
BASE COUNT         2087 a         1508 c         1111 g         2163 t
ORIGIN      
        1 tatattctct taaataatca aatttatttt taagaatcct gaaaataagt tttctaaatc
       61 ataggagcaa caaaaaaatt cgataattaa caaaaatcaa aatgattaaa tctagtgatt
      121 gaaacatatc atttcaaaaa aattttgtaa attcacccat aaatatcacc ctatttaact
      181 tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa tctttacttc ttggttttgt
      241 tttaataaat ttccttcaaa aatgcctgga gaaactacaa tgatcaccgt atctttttgt
      301 gcttgctcca acttcttcaa atctataata gctaacagtg tcccttcttt tacctcctgg
      361 ttactttcaa cccgtatctg aaacggactt ccttcgagtt caactgtatc taaacccata
      421 tggatcagta tttttctgcc agattcactt tcgatcgtta tggcatgtcg ggtaggaaat
      481 atattatcga ttattccttt aactggcgca tggatatatc catcatgatt cgttatagcg
      541 tatccaactc ctatcatttt tgaagaaaag acctcgtctt ttactgctgt taatggaata
      601 atttgaccac tagctacagc ttttagaatg tttcttgttt taaagaaatg cactttttat
      661 cacctctact ttaatgctgg ccaatatttt ttgtttactt tgatcatttc atctagtatt
      721 ttttttgcga ctctggcatt tggtacggtt tgattcaatg taaatgcttg taaagctttt
      781 tgataagaac cttcaaagta agcatctact agtaacttct ctactgcaac ttgtgcctcc
      841 atcaatcctt tatgaaaatc actgattggt tttcttagtg taattggctc cacaccagtt
      901 gcaccaacat atgccggtat ttcaacaact gcatctgacc gtagattcgg aatcgcccct
      961 tgattaggaa cgattagcat aaagcgacga cgatcatcat tcaacaagga aatagctata
     1021 tcgacgatat attgtccatg ctccccaaaa ttaaagttca atacttcttc cacaataccg
     1081 cggcgtattt tatcagccat ttctttggta tttttctctc gtccatccat gaccatatta
     1141 gcacgtgtat actctttgtc agtatgctca acgaccatat caggatataa gtaatactct
     1201 aagtaattat tcgggatatt acttggaaaa tttttcgtga tgactgacat catttgaaag
     1261 gttttttgcc aagttttatc tcctatatta aaatcggcga cttgcatttc ttttgttatt
     1321 agtttttcaa taatctctgg cataatttct cgttgcaatt ctttatcata aatcgatgta
     1381 taccagccaa agtggtttaa accataatac gtaacgatcc aattcttacg atcataaccg
     1441 taattcactg caatcgtttc ttcaattgaa atagtcatat cacaggcatt aatcattttt
     1501 acattcggat atttacgacg aacagcttca gaaacgatcg actctggatt tgtataattc
     1561 aaaatccatg ctttgggggc atagtcttgt atatgatcga caatttccaa taaacctttg
     1621 attgagcgca tcccataagc aaatcctccg agtccacacg tttcttgtcc tactaatcca
     1681 tgtttaagtg gaatcttttc gtccttttct cgcatctcca atcctccaac acgaatttgt
     1741 gagaaaacaa aatcgcatcc tgtaaaagca agctttggat catccgttgc acgaatgcga
     1801 atatttgaaa aaccttttct tttcagcata aatttaacaa tcaagtacat atccatattt
     1861 ctttcttcat caatatcata taaacgtatt tcagataatg gcaaccgttt ttcattgttc
     1921 aaaatggctt gaacgattcc tggtgtatac gtactcccgc ctccagctat agtgataatc
     1981 tgttttttca tcatcatctt ttcctttcta ttcttctaat aatttattga attctgccat
     2041 aacataagtc acttctaagc cgatgataat ttgataattt ttttcagttg gtttaatgat
     2101 cccgcttatc cctaattgct tgattcgctg ttcctcgacc atggtgggat ccactacctc
     2161 tacccgtagg cgtgtattac aactatagac atccaggata ttggcttttc ctccaaacgc
     2221 ttgtagcatt tttgttgcca tatattcata atttttactt gctaacccta actctgtttc
     2281 ttctaaggtt acttcattgc caaactctgc cttagcttca cgtcctaatg tctgaatgtt
     2341 tctcttattg atcaaataat aaaaagtaac gtaatacaag gcaaaaaaga aaattccgat
     2401 tggaagaatc aaaatagcat tggtagcgat tcgaaaattg atgagataat caacaataca
     2461 tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa cagacaatcc ctgctaaacc
     2521 ggcaaaaagt gcatggatca cgtacaacat aggagaaaga aacatgaaac taaattcaac
     2581 aggctcagtg atatttgcaa taaatgaagt tcctgcaccg cttcccatga ttcctttgac
     2641 catctcttta ttttctttat aagctgcttt atagatagct agacatatag ctggtatacc
     2701 aaacatcatt actacaaaaa aaactgataa aaacagtcca gcctgaggat ctccgttgat
     2761 aaagcgtgtc atctctcctc tgaaaacttc cttgctagga gatgtatagc ttcctaaatc
     2821 gtaataaata tacgtattca acacatggtg cagtccaaag gggatcagca aacgattcaa
     2881 aaacataaat atccccacac ctaatgctcc catatcaacg agcagttttg caaaattatc
     2941 aatcccaatt tggatgagcg gccagataaa gcccaaaaaa acagctgcga ttgtttgtag
     3001 aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa taagaaaaaa ctactggtaa
     3061 tttttgttct ttaaagcgat tatacgtcca tgccgctact aaaccggaaa taattccacc
     3121 aaaaatcccc atattcactt ctgggtccat aatttctaag ccttttttca aggtagcaat
     3181 cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta tttttgacaa aaccgattgc
     3241 aactcctata gcaaataaga tatccaagtt tccaaaaacg gcatttccag ctgcaccaat
     3301 tactgggatg ttcagcatat cgtcagctga taaacgcatg atcaatcccg ctaatggcat
     3361 cgctgcaact ggaagtagca tcgctcttcc aagcttctgc acatttgatt taacattcat
     3421 ttttattcct cctctattaa atatcctcat atgttactaa ctcaatcaaa ggatcatttt
     3481 tcactttttg agcaaatgct gaatgaatta aaaattcgac ttggtgggta cgaggataca
     3541 caaaacttga accatttaac aattcctgat ccaaatacgc tgcgtgacac atgacttcga
     3601 tcgttttatc ttctctcaat atcatgagta aacttgccaa atattcttcc acactcgtat
     3661 tgatttgtcg ttttttttca tcagtttctc ctacgacatc gaaacaaggc tcaaaataat
     3721 cgacatgacg aacttcatct tttcgttcaa agttgctccg aaccggcaaa tcatattttt
     3781 ttgctaatgt aacaactacc tcctgatttt catccaaagt atgagtatga tgatgactgt
     3841 ctaaatgagt tggtatgatc cccgcatgat aaattttttg aatctgagca ttccattcct
     3901 gatacagttg atctttgtca atgttaaatg gatgctgata ataagataag gatctgaact
     3961 ctccgctttc gataagagta tcgacagttt tgaggagtgg tttgccacac gtcaatgtta
     4021 aatggactcc tacacctagc ctaggcattt cctttcttag tttcacagca tgctcgaaac
     4081 ccggcatatt tgccatcaat gtagttgatg tcaaaattcc tcgctgataa gcatccataa
     4141 tcccataatt cacaccatgg ctatagccaa aatcatccga attaaaaatc acttttccca
     4201 tttcattctc cttctttttt ctactaaaat aggcgcaacc aaataatcat agtccattta
     4261 ctatgtgtca tttggttgcg cctatagata gttacgccct tattttaata gattcttgct
     4321 agatgaattg ccaaatagat gatttcattt tcttgaatgg caagttgaaa ttgctgttcg
     4381 atgatatctt taatctttag tgctgtacaa tacgctttgc tatgtttaaa tgtttcagta
     4441 aaaaaaccaa tattttctac tggcgtttct tcctgataaa tcaagcgttt aaccaggaat
     4501 ttgatatgaa ccattaagcg atgataagtc agtgattctt tatcataaac gtttgaatag
     4561 gatttttcta gatgggacaa aatcgtggtc gtcatttcta ctatttttaa gctctttaaa
     4621 ctattaggct ctcctagctc agcattaatg atatgcatag tgatgaatcc tacttcattt
     4681 gaccctaact cggtttgata tcgatgattg attcgctcga ccacttctac tgctatatca
     4741 tattcatttg gataaaggaa tctcatttct ggcaacataa ttgctgtgac atctctatct
     4801 tctctttttc gttgaatagc aaaataaata tgatctgtca atgtaatata gatatttgaa
     4861 tctaatggag aggagatcct agctgttatc agttcaatca cttcttccgc aaaatccata
     4921 aattcgattg gaatctgcga gactatctca ctgaatcgtt tgatcttttc tttagaatct
     4981 aatacaaata atttgtcagc atcctcaaca tcaaatggct tactcacttt agcagaaaag
     5041 ccaatccctt tccccatcgc aattttttct attccatcgt catcgatcaa tacagcatta
     5101 tgattcagct ttttcatcac atacaccgtt aattcacctc ctctatatga taaaacaaaa
     5161 caacttgcag actccttcat acatattttt gtctccaaac aatttttaaa gacaaaaaac
     5221 gacctaagaa agaacccttt cttaggtcgt gcctaattga atagtagcac cctacataat
     5281 ttacaaaaat agtttaacac ataataaaag cgttatcaat atctttttta ctcgttttat
     5341 gataatactt gtatcgaatt gatttggagg taattacata aaaacaaaaa aaatatagca
     5401 actctctata agaaagttgc tatattgata attataccgg cggccggggt cgaaccggca
     5461 cgtcctcaac ggacactgga ttttgagtcc agcgcgtctg ccaattccgc cacgccggca
     5521 aatttaaaaa taataactgg ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc
     5581 ccttgcctta ccgcttggct ataccccaag aataaagggc gaatgatggg aatcgaaccc
     5641 acgaatgccg gagccacaat ccggtgcgtt aaccacttcg ccacattcgc catcataaaa
     5701 gatgttattt aatttctcaa taaaaaacta agatgaagac atcttagtca ataccggcgg
     5761 ccggggtcga accggcacgc cctcaacggg cactggattt tgagtccagc gcgtctgcca
     5821 attccgccac gccggcaagt tgatttatta gtactaaggc ggtaaccgga tttgaaccgg
     5881 tgatgaaggt tttgcagacc tctgccttac cacttggcta taccgccatt atactattat
     5941 caatctataa aataaagaat aaattataga aactggggta gctggattcg aaccaacgca
     6001 tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata aaaagggcga
     6061 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
     6121 acattcgcca tggcaggggc agcaggaatt gaacccacac taacggtttt ggagaccgct
     6181 gttctacctt taaactatgc ccctataaaa tggaggagag tggattcgaa ccactgaacc
     6241 ctaaggaacg gatttacagt ccgtcgcgtt tagccacttc gctactcctc catggtggcg
     6301 cgggacagaa tcgaactgcc gacacacgga gcttcaatcc gttgctctac caactgagct
     6361 accgcgccat attttaaaat tattaattga ataacaagaa cgacggttcc gacgggaatc
     6421 gaacccgcga tctcctgcgt gacaggcagg catgttaacc cctacaccac ggaaccagtt
     6481 cattatggag gttaacggga tcgaaccgct gaccctctgc ttgtaaggca gatgctctcc
     6541 cagctgagct aaacctccaa cattcaatta aaatgacccg tacgggattc gaacccgtgt
     6601 taccgccgtg aaagggcggt gtcttaaccg cttgaccaac gggccaggta aatttgctac
     6661 ggagagtaag ggattcgaac ccttgagaca gtttggcacc atctacatga tttccaatca
     6721 tgctccttcg gcctctcgga caactctcca gagtgaaaga agctcaatac tctttctcaa
     6781 aatgagaact ccggcagtag gactcgaacc tacgacatca tgattaacag tcatgcgcta
     6841 ctaccaactg agctatgccg gaataacat
//
LOCUS       DAZULG010000127         6644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000127 DAZULG010000000
VERSION     DAZULG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.131"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG9CY_002472"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG9CY_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG9CY_002473"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG9CY_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS4938935.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG9CY_002474"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG9CY_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG9CY_002475"
     CDS             complement(2143..2373)
                     /locus_tag="ACG9CY_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS4938936.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG9CY_002476"
     CDS             complement(2603..3421)
                     /locus_tag="ACG9CY_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4938937.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG9CY_002477"
     CDS             3582..4271
                     /locus_tag="ACG9CY_002477"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4938938.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG9CY_002478"
     CDS             4285..5787
                     /locus_tag="ACG9CY_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4938939.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG9CY_002479"
     CDS             5800..6276
                     /locus_tag="ACG9CY_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4938940.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG9CY_002480"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG9CY_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZULG010000128         6352 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000128 DAZULG010000000
VERSION     DAZULG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.132"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG9CY_002481"
     CDS             complement(99..1229)
                     /locus_tag="ACG9CY_002481"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS4938941.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG9CY_002482"
     CDS             complement(1878..3080)
                     /locus_tag="ACG9CY_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4938942.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG9CY_002483"
     CDS             complement(3140..3343)
                     /locus_tag="ACG9CY_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4938943.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4549)
                     /locus_tag="ACG9CY_002484"
     CDS             complement(3485..4549)
                     /locus_tag="ACG9CY_002484"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4938944.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG9CY_002485"
     CDS             complement(4610..4717)
                     /locus_tag="ACG9CY_002485"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938945.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG9CY_002486"
     CDS             4880..5692
                     /locus_tag="ACG9CY_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4938946.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG9CY_002487"
     CDS             complement(6066..6257)
                     /locus_tag="ACG9CY_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938947.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1898 a         1302 c         1002 g         2150 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac ag
//
LOCUS       DAZULG010000129         6230 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000129 DAZULG010000000
VERSION     DAZULG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.133"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..633
                     /locus_tag="ACG9CY_002488"
     CDS             133..633
                     /locus_tag="ACG9CY_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4938948.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="ACG9CY_002489"
     CDS             730..2577
                     /locus_tag="ACG9CY_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS4938949.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="ACG9CY_002490"
     CDS             2580..3476
                     /locus_tag="ACG9CY_002490"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4938950.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="ACG9CY_002491"
     CDS             3526..3915
                     /locus_tag="ACG9CY_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4938951.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..>6230
                     /locus_tag="ACG9CY_002492"
     CDS             3902..>6230
                     /locus_tag="ACG9CY_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4938952.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2106 a          925 c         1336 g         1863 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DAZULG010000130         6175 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000130 DAZULG010000000
VERSION     DAZULG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.134"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG9CY_002493"
     CDS             352..1041
                     /locus_tag="ACG9CY_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4938953.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG9CY_002494"
     CDS             1028..2236
                     /locus_tag="ACG9CY_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4938954.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG9CY_002495"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG9CY_002495"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS4938955.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG9CY_002496"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG9CY_002496"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS4938956.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1895 a         1160 c         1201 g         1919 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgcggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattg
//
LOCUS       DAZULG010000131         6174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000131 DAZULG010000000
VERSION     DAZULG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.135"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG9CY_002497"
     CDS             725..811
                     /locus_tag="ACG9CY_002497"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4938957.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1622)
                     /locus_tag="ACG9CY_002498"
                     /pseudo
     CDS             complement(932..1622)
                     /locus_tag="ACG9CY_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1867..2781)
                     /locus_tag="ACG9CY_002499"
     CDS             complement(1867..2781)
                     /locus_tag="ACG9CY_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4938958.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2763..3143)
                     /locus_tag="ACG9CY_002500"
     CDS             complement(2763..3143)
                     /locus_tag="ACG9CY_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4938959.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3593..3880)
                     /locus_tag="ACG9CY_002501"
     CDS             complement(3593..3880)
                     /locus_tag="ACG9CY_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4938960.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3900..4124)
                     /locus_tag="ACG9CY_002502"
     CDS             complement(3900..4124)
                     /locus_tag="ACG9CY_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4938961.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4579..4749)
                     /locus_tag="ACG9CY_002503"
     CDS             complement(4579..4749)
                     /locus_tag="ACG9CY_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938962.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4764..5297)
                     /locus_tag="ACG9CY_002504"
     CDS             complement(4764..5297)
                     /locus_tag="ACG9CY_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4938963.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5318..>6174)
                     /locus_tag="ACG9CY_002505"
     CDS             complement(5318..>6174)
                     /locus_tag="ACG9CY_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4938964.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1634 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa atataacccc tggcaataaa
     1501 aacctcttaa atcgaatttt agagcctttt aatgaatatc ttgtttgata agccttatag
     1561 ctaagccaaa taattcctaa aatactggca aaaatgagta gatagggtaa atagaatgcc
     1621 atttctattt attctcccca taccagccaa aaggctaaca gtgtcatgat taagcaacat
     1681 aagggggcaa gaatatccat tgatcgctgc ataattactt ttttctgttc tttatttcgc
     1741 tggtttaata gcgcatggtg gaattttttg acttgtgaat caattgtagt ttgtattctt
     1801 tctttggtat aaggtgcaag aaaagcacgt tgtttcttta tttcattttc tatgtcttgt
     1861 aacgttttag aacgataatt cttgttggta gtctgctcct ctttcaacgt gttggtcatg
     1921 gtttcttcga gtcggtctaa tttccggttg ttcagtcgta agagttcgtt gttctcttcg
     1981 tacttctttt gcagtttcgc gaatgctagc tcgagctctt cgtagctcgt tggttcgttg
     2041 ttgattgtag gctcgttctc gtctgtccag ctcatgtaaa atcgtctcct tttcgtaatc
     2101 ctttcctagt ttgttggatc gtactttttt ctttttgtgg atcaattcgt aagtaaaatt
     2161 tttcccacga ttcaagcaat gaatgccgaa tgttttaaga gaatcacaaa attggttata
     2221 cgttcgagtt tttttgtttt tcattgtttg atcaatcgca aaccgaattt cgtctttcca
     2281 aacaggttca tttttttctt ttaatttgat ttcagacatg gttcgttttt cgggtttctt
     2341 ccctcgttca gtgattgtta agtcatgttc aagacaaatc tcgtcattta ggtcataaca
     2401 tttgtttaaa aatgcttgac catgggcttg aaatttgttc ccagtttcca gattgacgga
     2461 gttaatgaca atgtggttat gaatatggtc tttgtctgta tgtgtgtaaa tcgcgatttg
     2521 gtggtcaggg gctatttttt tcgcatactc tgcccctagt tgattggctt tttcagcggt
     2581 aatttctcct ggtcgaaaag actggatcaa taagtgtgct tgtacacgat cgtcttttcc
     2641 atacagcatg cgaatttgtt tcatttcgct tttggcataa tcaatgtcac agtttatccc
     2701 tgttttgacg gttgctcgtg gttcggcgta attgatcgct cggctacaag aagtggttcg
     2761 gcttacttta actgttgcca tatcttttgt agctcctttt gcatgatttt taggttttcg
     2821 gataagcgtt gggcgtcgcc aggatcaacg tcgtgttggt gtgtgttgca ccacttggca
     2881 atttgattga tattcccgcc agctttggct aaatcgcgcg cgattgcctg agcaatgtct
     2941 ggcgccactt tcggtgtcac cagacgcgcg ttttgtgcct tggctttcac gaaagccggc
     3001 acactcattt gcaaagcttc agctgactgc ttcagctttg caaattcgga ttcgctcact
     3061 cgaaaactga tttgttttgg ctcggatcga taggtatatt ttttagaatg attgctagcc
     3121 aaatattgat tttgttcgct cataagtccc accctttcag tacgtcctac ggacgtatcg
     3181 ttcgcaatac cattgtacct aaaaaggtac aataagtaaa gctcactaaa gctcactaaa
     3241 gggcttggca agctctatat ttgctagcca aatacctacg tatttggtgc aaataaccgc
     3301 ttgtcaaagg ggcgcaagct cccccttgaa tccccctccc ccccactctc cacaatacaa
     3361 aaaagactag caaatgctag ccactttttg taaagtttcg cttgtttcac cttcggtgaa
     3421 attgttgcaa atcaataatt aggtagagct acattttttt cgttgttcaa agattcttat
     3481 gccatttact attaataaat ctataaatat gataattttt cggctctata atttatggga
     3541 ctgatgaatc agaattgatt cttcagtccc attttcattt tattccaaag aattaactcc
     3601 aaataccaat agaagcccat cctctatata cttgagattc cttactaaca atttcttgta
     3661 attctattaa agatttaggt aatccaaatt tatgttttaa aatatatctt ccaagaaaac
     3721 tacttaattt catactaact tcaccaacac tttttccctc actgatttct ttagaataaa
     3781 gaaagagctt ttcttttaag tcaggatagt catctatagt tgcttcattg tatactttac
     3841 taattgcatt taaaagtttt tcttctttag taaaatccat tataataacc tccctaccac
     3901 taccttctag gtgcccatgg accatgatta acccaaccat taacaataat ttttccaatt
     3961 tctcctttag cttgattcca atctacccaa catttttctt tgttacaata aagaccattt
     4021 ccatagtaag tagctgcatc aacttttatt cctgtaccga tgccagctag acaagttcct
     4081 aatattccta gaataacaca atgttttaat actttctttt tcatgattgt ttttcctttt
     4141 cgttttttta aaattgcttg ttatattaaa taatcttagt aattttatta tataacattt
     4201 tattatataa caatgattaa cgtcataaaa agtagttttg aattcataat cgtttaaata
     4261 aaattataag atcagttatt taaacagcta gtagacataa tgtgtatact accaagtaac
     4321 gcctgtacca agggatccag gcgctgattt tgtgaatttt tattggtcta ggttaaaagc
     4381 tctttttaag gcttggtttt ctgcggttgt ttctttctaa ttctttgtga atattgatcg
     4441 gtcatttttt aaatacttgt tatttttgtt tgtgtttgtt atgcttttta catatctcat
     4501 attcatttat tattcttttc taaggttaat aataggataa tctctttcag aaagactgct
     4561 cgtacgagca gtcttttctt atttatcaag tttttctaca aatacttcct cattttgact
     4621 gttgtagtaa ttaggattga gttttagttc ccctttttta tttcctttgt tggccactga
     4681 taataacatt gggctaaagc ttaatattcc tagtaggata gaaccgaata gcaagtgttt
     4741 ttttgtcata agtatttcct tcttcatttc catagctgcc accatttttt ccaattcttt
     4801 gtttgttcgt ccattgtttc tgttgcttca gaaagagtcg ttgaactatt ctctgaagac
     4861 tcttccgtat aagtcaactg cagttgctcc tgaagttttt cgatttgttg gttggcttgc
     4921 agggtgagtt gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctttg
     4981 agggcaagtt gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt
     5041 gcaaccacgt ttttgtttgg tatataagca tttttatcgt tgttttgttc atgtttatgc
     5101 aacttatcta gcaacctttg attgtctaag ttgcgagcaa gttgcaatac tccagaacgc
     5161 aatacaacct tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata
     5221 tgtttcgata ttgcttgcct actcatctta aaaagatctg acaattcctt tattgtaaag
     5281 gtttcttgtg gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat
     5341 cagataataa actttttttc tctttttccg aaagtgcgga ctctgtttgt ttttcaagag
     5401 cttttttctc tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct
     5461 gtttgatttt ttcgttaatt gttgtagcgg gttcttcgta ttctttttgt ttatcagcta
     5521 aagccaatgc tttattttgg tcttctttgg ttggtgcatg tttgttaaag tcgtctgctt
     5581 ttttgatctc aggtttgaac gtaaattgat agccaataac aggttttccc cgtccttttc
     5641 cgtatttctt tttgattgtt agtcctttga agattgcagt tagttcttga cgtataggtt
     5701 ttataactcg tttatctata tcaggttgct tataactttt aggaatatct aataattcaa
     5761 gaaagtcttc tttggagaaa tatgcgaaac ctttggttct gtattgcttt aacagacgaa
     5821 acatagtctt tgcatagcca ctttgtagct cgttgaattg ttgtaaagaa tagcgtaccc
     5881 actcgtctaa attatttaac aaaggcagtg ctttttcgta aattcgtata tctacgtaag
     5941 gattttctac gtccccgttg attttaaatt ctgtaaacat tacaaacata tcgtaagaaa
     6001 gcccagactc acttctacga ccaaagcgta aagataagat tttttcataa gttgctttta
     6061 aatcttctac aaatcgtaca tttgcagttg ctttgtagtt acttaaatct tttagttggt
     6121 caaacgtaaa acgaactgtc ttatcccctt tttcacgcat tctagacacg atgg
//
LOCUS       DAZULG010000132         6173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000132 DAZULG010000000
VERSION     DAZULG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.136"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..116)
                     /locus_tag="ACG9CY_002506"
     CDS             complement(<1..116)
                     /locus_tag="ACG9CY_002506"
                     /inference="COORDINATES: protein motif:HMM:NF045046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HFS4938965.1"
                     /translation="MNILILSCGRRCELVNAFKTALNNNEMEGKIIGADCSEL"
     gene            complement(128..1945)
                     /locus_tag="ACG9CY_002507"
     CDS             complement(128..1945)
                     /locus_tag="ACG9CY_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFS4938966.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(1959..2723)
                     /locus_tag="ACG9CY_002508"
     CDS             complement(1959..2723)
                     /locus_tag="ACG9CY_002508"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS4938967.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            complement(2751..3449)
                     /locus_tag="ACG9CY_002509"
     CDS             complement(2751..3449)
                     /locus_tag="ACG9CY_002509"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS4938968.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3461..4240)
                     /locus_tag="ACG9CY_002510"
     CDS             complement(3461..4240)
                     /locus_tag="ACG9CY_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS4938969.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4256..5200)
                     /locus_tag="ACG9CY_002511"
     CDS             complement(4256..5200)
                     /locus_tag="ACG9CY_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4938970.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5246..6025)
                     /locus_tag="ACG9CY_002512"
     CDS             complement(5246..6025)
                     /locus_tag="ACG9CY_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938971.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1614 a         1292 c         1115 g         2152 t
ORIGIN      
        1 agttcagagc aatctgctcc aatgattttt ccttccattt cattattgtt taatgctgtt
       61 ttaaatgcat tcactagttc acaacgacgt ccacaactta atattaaaat attcaatttt
      121 taatctccta tctattcgaa gcatttgcaa aagtgacaat gtctttagcc aattgttcat
      181 cgtcttgcgg caggctcttg acaaaatcca tcactttttg tattgagtaa cctttgatgt
      241 ttcctacaaa gattttctca taaactgctt catcatttct ttccttatcc aacaatagtt
      301 cttcatatag tttttcccct ggacggatac ctgtttcaat gatttcgatt tcgtcttcgc
      361 tatacccgct caatcggatc atgtttttcg ctaggtctac aatcttcact ggttcactca
      421 tatctagaat aaaaatttcg ccaccttttg ctaatgcgcc agattgaata actaaacgac
      481 tagcttcagg aatcgtcata aagtatcgag tcatacgaaa gtctgttaca gtgattggac
      541 caccttgagc aatttgttct ctgaatactg gaatcacact accgcgagaa ccaaggacat
      601 tcccaaatct gacagctgag aatttcgtac agccttcttc attcaatcca gtcacaatca
      661 tttcagcgat tcgttttgtt gaacccatga catttggagg attgttagct ttatctgtcg
      721 ataccatgac gaagtttttc acttttgctg ctttagctgc ttctgctaca ttttttgtac
      781 cgaaaatatt gtttttgact gcttcttttg gattatattc catcagtggt acatgtttat
      841 gagcagctgc atggtagacg atatctggat gatattgttc catgatctca ttgattttct
      901 cgcgatcttg gatatctgcg atgatcggta cgatttctgt aggacaattt tgatgatgcg
      961 tgcgtaactc acggtcaatc aagtagatcg agttctcccc atgtcctaaa agcaatagct
     1021 ttgcaggatt gaattgaatg atttgccgac agatttctga accgatcgat cctcctgcgc
     1081 cggtgacaag aatgactttg tctgtgatct gatctttgat ggattcaata tccagcttta
     1141 cctcatctct tcccagtaaa tccactacgt caattgtttt taattttgat acgctaattt
     1201 tccctgaagc aagttcttct atagaaggca tcgtgttgac tttcacacga gcggattcga
     1261 ctaactcgaa gattcgtcgt aatttttttc ttgataagga agggatagcg atcgttacca
     1321 tttgaatgtc atatttttca atcaattccg gaatatcttt taatgcacct aatacttttt
     1381 ttcctgaaag atacgtattt cttttatttg gatcatcatc tacaaacccg acaacgtgga
     1441 tatcttgagc cgttttggat ccaagaaagc tgttgtataa aatacgcccg ccttcgccag
     1501 caccaacaat caatgtattt ttagcagaat ctgctgatac gtatctcata ttttttgttt
     1561 cgatgtagat acgccagatc aatcggctac cgatgatcaa taagagcgaa agcagataag
     1621 caaagataac taaccgtaaa ctgtatcgcc gattaatgaa cagcagtatg aggatacttg
     1681 aggcagcact tgcagatagt gaactaaaaa ttgcaatgat ctctcttaaa tttgtatagc
     1741 gattgatccg agtgaaaact ttgaataaac taccataaaa taaataaaaa ccaatcgaca
     1801 aaccgataga gattaaaata agatccattg gtatagatac aaacggcttc atgaatttag
     1861 ctgctgcaat atttgcagca attaataaca agctgtccac aaaaaccaat gtggcaattt
     1921 ttcttcttct tgttaatata aacacttttt aattccccct aaaataaacc aaacttcttc
     1981 ttttttacct cttcatagtc tggataactg atttcatccc cgttgatcag atcctttgca
     2041 acttgcttca tctgtactac tttttcctta ccaaagtcct tggcaatctg ctcatagcac
     2101 tcttttaagt aaaaggtgcg cttagagact ccgtgtgcat cagaagcagt catctgcacg
     2161 agattatgtt tgaccattgt tttagctgtt ttctgaatat ctttgccaaa taccccaaca
     2221 taacttggcg cagtaagttg ggccaaacac cccatatcta ggaacggaag caaatgattc
     2281 gggtcttcgc gaaaatgcgc gttgcgttct ggatggacga tcactggtgt ctttcctagt
     2341 tctaacaact caaaaaaaag ccgttctgca tataacggca cttccagtgt cggaaattcg
     2401 atcagaagat atgtatcatc taaatctgta aataaaattt catctcggtg gatgtcttcg
     2461 atgagcgttc cggtaatacg tacttcttgt ccttccaata acgtcaaagg caactgtctt
     2521 ttctctaatt cggcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg
     2581 tattttccat tattgtggtg tggtgtgcat aaaatatgcg tgatgccttg ctgaatcgct
     2641 ttttcagcca ttgcaatact tgcttctaag ttttctgccc cgtcatcgat tcctggtagg
     2701 atatggcaat gtagatcgat catttctctt gtctcctctc ttcttattcc ttaatttccg
     2761 tagtaataac cagcatcttt gctatgttct gctccgttgt atacaacacc taaaactcgt
     2821 gcttgtacca tctctagcag ttcttttgct ttattcaatg attctttacg tgatgtattt
     2881 tcacgtacga ccaataaagt gccatccgtt tttgaagaca tgatctgtgc atctgttacg
     2941 gctactactg gtggcatatc gaatatcacc acgtcaaata actgtcttgc ttcgtctagg
     3001 atctgatcca ttcttggcga acttaacaat tctgatgggt tgggtggctt aggtccacta
     3061 ggcaaaatcg acaagttctc gactggcgtt cgttggatta catctgctac acttcctgat
     3121 gaactaagtg ctgtacttaa tcccctggca ttgttcagct ggaatgtctt atagacgacg
     3181 ggtttacgta aatctgcatc gactagtaga actcgttgac cggatttagc aaaaacgact
     3241 gcaatgtttg ctgcagtggt ggattttcct tctccaggac ctgatgaagt cacgacaatc
     3301 gttttgattt gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat
     3361 tgttcggcga ttggtgaaga tgggtttgtt aatgtgatca aactgactgc atgagtttgt
     3421 gctgcttttt gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc
     3481 tgcgtgatgg ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gttccttttt
     3541 tacggatacc agcgttcaat tctttcgctg acatttctgg aactgttcct aagattggga
     3601 atcctaatgc atccgtcaca tatttttcat ctttcactgt ccgatcaagt aattcgaata
     3661 agaatgccag acctacgcct accatcattc ctaatactaa gccaatcact agattcaatt
     3721 tattatttgg cgaaacaggt gtcgtattgg caactgcacc tgaaataatc gagattttat
     3781 cgacattcat cacgtctttt gcgttctctt ggaaaacttg tgaagttgtg ttagcgatct
     3841 gttgagcagt cactgcattc gtgcttattg cctggatcga gaacatttgt gaattttgtg
     3901 actgattgac ggagatcgca tctttgattt gtccagcagt cattttcaca ttgtcttctg
     3961 tttctaaacg atctttgact tcatttagta ctaaatcact gacgatcata tctttatacg
     4021 tattgatcat ttgcaggttc gtattgacat cattgacatt tgctgtgtct gattgtggca
     4081 gtgtcacgat caattgtgct tgtgaactgt attttggtgt caagatgaaa aatgttacga
     4141 ttcctgagat ggccaagcca gcaaacattg agatgatgat tgttaccaag tgtttgcgta
     4201 atgtttcaaa aatttctttc aagctgattg tctcttccat tttttctcca ctttcctatt
     4261 tattctctag attcatttga tcttttaatt cttttttcac acgatcaagt tcttgatcgc
     4321 tgactttttg ataggagata ttatcttgca tgaagccttc tccttgcagc tgatcttgct
     4381 tgatcgtacc aaatgcctcc cgataatcta aagcgatttt tttcatttca tcaaagctca
     4441 tgtctgtttt catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt
     4501 ttaaactcaa tacttttttt gctacacctt ctacgatctt tctttgacgc tcttgtctac
     4561 catagtcacc atttggatct tcataacgca tacgagaata agataatgcc tgatccccgt
     4621 tcaatgtaat tttaccgatt ggaaagtcat agccatcttg tgagaaagtg aggtcattat
     4681 tgacttcgat accaccaact gcatcaacga gttcctttag ccctttcata ttgatagaga
     4741 catagtgatc gattgggatg tctacatatt tttgtagggt atccatcgcc atggcagctc
     4801 cgccgaatgc gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat
     4861 acgtatctcg aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actgtggcaa
     4921 ccatcatcgt atctgatctt ccttgatcga cccgacctaa atctccagta tcaatgccca
     4981 tcaatagaac agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt
     5041 gggttcgttc cactgattcg taagttttct caatagaatg tgagagatcc agatataatt
     5101 ttgctcctac tcctacaatt gctaatacta atacgataag aactgctaaa ataatcagaa
     5161 atattttttt ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac
     5221 cttgcttgaa tcaacgtttt aactgtcaat aacttatatt gctatagccc atttgctgag
     5281 caacagtttg tgcttttgtt gcgtatgctg cgcttatttt ttcattccgg atctgctcta
     5341 tttgactgag cgccgtcaaa tattgatcgt aagtagcggt ggcagtcact tgaccatcct
     5401 tcaacatcgt atcgaataac tgatctagtt gcgtgacttg cgcgatctga gtatcagcga
     5461 atcctagata ttgcataaca accgttttcc aaggactatc tttaaaaaag ctcatatttt
     5521 cacgtacacg agcgacatct gcacttgtca ccttttcttt gatgacgaca tcgtctacag
     5581 cttgctgcca gtttgataca tttttagtaa ataaatcgtt gacagctgtc tggatcttca
     5641 gttttgattc tgcactatct aactgtgtca cgattgcttt cttctcatct tggattgcaa
     5701 cagcttcttt aggcaattct gattctttga tgtcgtaatc atctgctgaa agtttgacag
     5761 aggatagctt atttgacaaa cttgtgattg tttcttctgt tatttcaggg tttagaaaat
     5821 ttttttcttt tgtactatac agcttgtcta cttcttttga taaagaagtc aaggtgtctt
     5881 tttgattggt gatggcagat tctgcataat tgatccgttg ctgatgttgc cgctgattat
     5941 tgatatggta accaaacaaa cagatcgcaa tagcacctgc acctaccgcc acagcagtca
     6001 atgacttgac gaatggcttt tccatagatt cactcctaag tattgatgat agataaatta
     6061 agatataatt cacatacata aaaatgacag ctttttattt taaaaaacat aattaaatta
     6121 atatattcac agaaatattt gtaaacaagt tttccctata taatcatacc aca
//
LOCUS       DAZULG010000133         6068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000133 DAZULG010000000
VERSION     DAZULG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.137"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACG9CY_002513"
     CDS             832..1566
                     /locus_tag="ACG9CY_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4938972.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACG9CY_002514"
     CDS             1582..2751
                     /locus_tag="ACG9CY_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4938973.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACG9CY_002515"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACG9CY_002515"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4938974.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACG9CY_002516"
     CDS             3841..4767
                     /locus_tag="ACG9CY_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS4938975.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACG9CY_002517"
     CDS             4786..6036
                     /locus_tag="ACG9CY_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4938976.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAZULG010000134         6051 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000134 DAZULG010000000
VERSION     DAZULG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.138"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(277..612)
                     /locus_tag="ACG9CY_002518"
     CDS             complement(277..612)
                     /locus_tag="ACG9CY_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4938977.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(599..883)
                     /locus_tag="ACG9CY_002519"
     CDS             complement(599..883)
                     /locus_tag="ACG9CY_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4938978.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(939..2462)
                     /locus_tag="ACG9CY_002520"
     CDS             complement(939..2462)
                     /locus_tag="ACG9CY_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4938979.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(2455..3630)
                     /locus_tag="ACG9CY_002521"
     CDS             complement(2455..3630)
                     /locus_tag="ACG9CY_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4938980.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(3634..3819)
                     /locus_tag="ACG9CY_002522"
     CDS             complement(3634..3819)
                     /locus_tag="ACG9CY_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938981.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(3785..5479)
                     /locus_tag="ACG9CY_002523"
     CDS             complement(3785..5479)
                     /locus_tag="ACG9CY_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4938982.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(5476..5949)
                     /locus_tag="ACG9CY_002524"
     CDS             complement(5476..5949)
                     /locus_tag="ACG9CY_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS4938983.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
BASE COUNT         1639 a         1289 c         1001 g         2122 t
ORIGIN      
        1 ttataacaaa aaaaacacgt ccacaacaga cgtgttcttt gatttctaca caatcagctt
       61 gccaaaacca tccaatcagt cctagcatgc ctgtattaaa attgaacgtt catcatctat
      121 catctttctt agtccagcat gtttgcaacc acacctacaa ttttcagtcc aacaatagat
      181 ttcgactagc taagtcttgc aaaattcagg cagctaaccc ttcatttact accttaatta
      241 tactaaatac ccacaacgtt tacaagtgta atcattctaa gaaatctgtt tagctatgat
      301 cgttgtataa tctttaaacg ccgttccttc attgattgaa ataattttat aactcttgtt
      361 tttccaagct actttcatat cgtttgttag ttcataatct tgatcgtatc gaataataaa
      421 cgtcaacgtt ccttcaagaa tggtacctac actagcaagc acgtctttta gcatttgtga
      481 acgcacatta gcccaacatt caaatacagt ttcttcacta tccaccatct gcccatcttc
      541 atcttttacc cgttttgtct ttttaaactt gactctctca gtcaattcat ttacattctt
      601 aatgattggc ataataagca cctctcattt gttgtactaa tgcttgtacc gttactggta
      661 tccgttcgct gctggcttct tgacggttca aataccaatg ttgagtaagt aatgacaccg
      721 ccaaatcaaa ttgctgataa ttatccatat ctttatccgt tgcgctacta tcaatagcac
      781 tttttatata ttcgcctgct gtatcaatca attgttgaat aaagtcatca tcaatagtgt
      841 gatcgatccg catacttttt tttatgctat ccaaaattac cattttatct ctcctaatga
      901 aaaaaaggca gtgggataaa ccgccgccca atatttttct atacttttgg tgttacaact
      961 ggcgtaaact caatatacac tgaagcattt tgatcaattt tcttataatc gttgcgaacg
     1021 atcactgcaa gtccttgtga gtagtaatcg aatttttccc attgagtcgt tacttgattt
     1081 ctacgagcaa caaagataga ttgtgcaata tcccccataa tcatagggaa tgttccagct
     1141 ttaggattgg caaacaatgt atctgcaatc aatactactg gcataccgaa taatgattta
     1201 ccactaggtg ctgtcacatc gggttgtaaa atgtaacgtc catctgaatc tttcaatgta
     1261 tctaggtggt aatatccgct ttggtttaga attaccattt tatttaatgc tgggtctaat
     1321 gccacattgt atagttgttt caaatcgtcc aacgtagcag ccgttttctt agtgaatgtt
     1381 tttaataaat ccatgatatg cttattgtca gtgttttcta ccaatttcgc taactgatct
     1441 ttgacctctt gtacaatatt cactgctgaa tcttccacaa cctcatttga taaggcaatc
     1501 ttaccagcac gagtttctac tttataatca actgaagtaa acatttcagc gtcaatatca
     1561 ccaatttcag caagttcagc ttttgttgct aacactgctt gttggttagt tgctactgga
     1621 tatttacctt gtccatttga tactgttttt actgttgcat attgagctaa gttataattt
     1681 gaacgtttca aatcaaagac ttcaccgatt acttcttcgg gaacaactgc cgctgcattt
     1741 acagtagtga ctccatcacg cacttcgcct tgtgaacgga tatattcttc atacccacgc
     1801 aattctgttt tttcgttatc gattaaagtt ttttccatgt tattactctc cttttgtgct
     1861 ttagaagtgg attctaagct attgtttgtt tgttttgctt ggttgttact cataaacgat
     1921 tcgtaagaac gcttatttac ttggacattt gatgaatcat aagcgggaac ggtcacgaca
     1981 ctaatttcat taagtgcttt gattttatct attgatcgtt caattgtgcc atcttctttt
     2041 ttgtcgaatg aatctaatcc aagcacgaac ccaaaggaca tagaatcaac aacccctttt
     2101 gagatattct catacacatc atttgaatag gtcgtgtcgg ttagctctgc ttcaaaatgt
     2161 aatcctgtat catcgacatt caatttcaac gtacctgctt tgacactagc tagtggcttt
     2221 gaataatcgt gtccatataa taagaacaca tttgataaat ccacctcttt taatgcttcg
     2281 ggtgtaatca cttctacaaa gccgcctaaa tcttttgacg gttgcccgaa tttcaacgca
     2341 tagcctgaaa tagttttgcc gtctgttggt tgtttttcat tttctttctc tactttggga
     2401 gaatcggctg ttagctcagc ttcttctgtc agccgttttt ccttttcttc ttcattattc
     2461 aactggtgta actccttcct gttcttttaa ttgatatttc tccaacgtat ctaaatacgt
     2521 aaagttcaaa cttgctagta aacgatcgcc accatccatg gggggtagcc ccattttcga
     2581 ccgcccctca ttaatcgtga gaagtgaccc tttgacctga tccaatacat tttttacttt
     2641 tgtttcagga tcagtctcta gtaactgatc ggaattaaag cggatattgg tttcaagttt
     2701 cgtatctaat tcactcacaa acacattaaa gtaatgaatc agcgtacttt ggatatactg
     2761 gagattactt tgaacggtgc tagaatgttc attttcaacg cctaaacgct caatcggtac
     2821 accaaacgcc ttggcaattt gttttgtgtt ccaatcattg gaattgataa gcttcaaaac
     2881 atcggtgttt atttctaatg ttttgtagtc cattgtttca tcaagaatga tcgttctaag
     2941 agcattatct ccactacttg aaccgttggc ttcttcaaac ttttcacgaa ttgcgttctt
     3001 tgctgatccg tctaaatctg acttatgaac cttcaaaata cctgacccac tgacaccacg
     3061 agtaaagaaa ttgtgcaatg tgcggttgcc agcttgttga attttcagtt catcacgtaa
     3121 cgcataaagt ggtggtaatc ctgttaaacc atcttgggtg aaatatttga agtgcaaaat
     3181 atcgctagac ttcacacgtc tttttttatc accaatctga taagacaacg taccattatc
     3241 taattgagta accgttacac ttgagttagg taataagtga atttcttcta ccttttcacc
     3301 gttgcgttta atctctgcaa agctattacc gtttagcaac atattgaccg ccaaagcaaa
     3361 ctttaaatgc caaccatcca tttctgaatt aggtttctcg tttagtaact tcaccagttc
     3421 attatcagct tgtggcatat tgtttttgac caattgaatc ggacttgaag caatatcact
     3481 agcaataatt cgaacggctg caaacacatc actatttctt aatgcacgaa cgcttgtaaa
     3541 gctggtggtg taatcatccg attggattga taccacatgt tgtaggaaag gatctcccgt
     3601 tgtattttgt ctcgctttaa aaaatgccat cttttaacct cccttctgtt ctgtatttag
     3661 catgaccgct accccaataa gcactatgcc cagcgctaac aagccatagt agacatttgt
     3721 tagtaaggtg atagcaacca acacacacac caatccaaga agtaaaagaa cggtatgaat
     3781 gtaattagaa agaaaattct tcacttgtat aaaattcatt gtctgctttc gctccttccg
     3841 ttgtggtgaa gtaatccatt gcaaacacat aggcattgat taatgcagct attggatcaa
     3901 ttttgttact gttctttgat ttattaatct gaatgccgtt gttatccgtt tttaaaatag
     3961 cgttgttcac tgcatgggtg agaatcgtat ttttattgtg aataacgttg ccttcataaa
     4021 cttgttctct aaaagttcgg gtcggaacgt ttagagtaat cgttccttgt cttacttcta
     4081 acattgggta gttggctttt tcagctttac tgataagcga attagcgtta tacgggtcat
     4141 aacaaatccc catacattcc aaatcatttt cttgaatcaa atcttgaata aactgaaata
     4201 cttggtcata gtccacgatt ccactttcta attgagtaat ggaacactca ccagcttttt
     4261 ctagttctcg ataaggcaaa ccgtcgcgct tttctttgtc ttgaagccca tatttggtgg
     4321 ctacaaagct atgtgaatca caataaagtt cgccgttatc tagcggtacg atccatgaaa
     4381 cgctagttaa atcatctgtt ttagataaat ccactccgat ataaacgggc ttgcctgtaa
     4441 tatcttgtgg ttctacttcg gttgcctgcc agtcttcagc aatcatatag ctgtcctcac
     4501 tcgcttgtct ccataaattg aagtttttca ccaatacaga attaaggtta ttttgtttca
     4561 aagcaaggtt cacatcatct tgaatggttg gaatcattac ttttttgatt tcttcacttt
     4621 caaaaatcgg attggctttg atccatttct cttgatcgtg aatttcttcg ggatcgtcca
     4681 attcccatat cgctataaag tacctgtcgg cttgttcttc gccttttagc acacgatcta
     4741 acaataagta ttcctcgtac ataggaacgt ttaaatcaag cccagcggta ctaataacac
     4801 acaataagcc gttcttttgt tgggtcattc ctgacttaat gacgttgtac gttttacgag
     4861 tttttgcttc gtgccactcg tcaagaattg cggttgttgg tgcaaaccca tctagcgtgc
     4921 tggtttcact agccagtgcc atagcgaacg aattagaggg caagtgctga atcttagagt
     4981 tcataatttt cagttgtggt cttaaaaact tactagactt gaccacgttt ctaagtgaat
     5041 tagacagcat atcatagccc aatttagctt gtttcaaagc gttacttaca aacaatactt
     5101 gccttgcttc tgctggttct tgttccataa tcaacgcatt ggcagccatg ccacttgcga
     5161 tatacgtttt cccattctta cggctcatac tggtaaacgc tcgattgaat cgcctgtaat
     5221 tcccttcttt agtacgccag ccataaagac tgcctagaat gaatttttga aatcctaaca
     5281 tcttgattgc tttaccgtcc gtagatggta gcaactcaat gaatttaatc gctttggtgg
     5341 cttgttcttc atcgaacaca taagggaagc tatctgactt agaacgctcc aaatcgctta
     5401 tatgacgctc acacgcttgg ataattttct tgggtgcaat cagcttacct gataggactt
     5461 tctcaatgta atcattcatg aattcatcaa ctctttgaac gggtcttcgg gttcatcatt
     5521 tttaaccttg ttcaaagcta gttttgatcg tgattctaag gtcataccta gtgaggtggc
     5581 ttgttttttc aaatcactca tagcttgtga ttgaacccga accgctggat tggctttctt
     5641 tactccgttt tcgcatgtga taaacgtacc tgattttctt atctcggctt cggctgtttt
     5701 cattcgagca aaggctaaac aataggctac caatgaacca taatctgctt cactcaatgg
     5761 ataatctttt tttaatgttg gtacgatacg attccattca cttctagcgc tcgctgccat
     5821 ccagtcaggg ggcgtgatat ttattaatgg ttgctgcttg aacaattctt ctctagcgtc
     5881 cacacggatt gctttttctt cacttgatat attgcctttt gtatcgtgta atattttagg
     5941 gtgtcccatg ttgtcctcct ttcagacaaa agaaaaagga acgtaagcaa ttaagctcac
     6001 attccttcgt ttttcgatca gaatttttct ttatctaatt tatctatata a
//
LOCUS       DAZULG010000135         5972 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000135 DAZULG010000000
VERSION     DAZULG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.139"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(184..669)
                     /locus_tag="ACG9CY_002525"
     CDS             complement(184..669)
                     /locus_tag="ACG9CY_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938984.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(808..1023)
                     /locus_tag="ACG9CY_002526"
     CDS             complement(808..1023)
                     /locus_tag="ACG9CY_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS4938985.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1247..2047
                     /locus_tag="ACG9CY_002527"
     CDS             1247..2047
                     /locus_tag="ACG9CY_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4938986.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2066..3934
                     /locus_tag="ACG9CY_002528"
     CDS             2066..3934
                     /locus_tag="ACG9CY_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4938987.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3927..4418
                     /locus_tag="ACG9CY_002529"
     CDS             3927..4418
                     /locus_tag="ACG9CY_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS4938988.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4406..4960
                     /locus_tag="ACG9CY_002530"
     CDS             4406..4960
                     /locus_tag="ACG9CY_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS4938989.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4978..>5972
                     /locus_tag="ACG9CY_002531"
     CDS             4978..>5972
                     /locus_tag="ACG9CY_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS4938990.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2094 a          950 c         1205 g         1723 t
ORIGIN      
        1 tgaagtagat accaaaaatt tatcaagaga gcaagtagag aagtgagtat attcataaga
       61 gaaatagctg taacgaatta ttaggttaat gcaaatgctt atttatactc cgtataactt
      121 tctcagaaaa aatgatacca ataatgactt gctagtactg taaatccgta taatatcatc
      181 taatcaaaat ggtgttccat ttgcatctgt catcgtaatc gatccttcgg gtgacactcg
      241 ataaaaacca acgcttccag atcctccttg ggaacggatt tgtttgctgg aagctgtgaa
      301 caaataatct cgatcaattt tttgataaaa gctaataaca atatcgtcac tttgtaattc
      361 ggaacggtgg gcaataagaa atagaagtgc tttgtcagag ttttctaatg gaagagaagg
      421 tacagtgctt tcttgcattt cagatgttga agaaatgcta aacgactgaa tacttgattg
      481 gctatgttgc gaatctacat taccagtaga agtattagta atggaagtag tagaagtact
      541 tttagttgaa ccaacctcag ttgttgaaat ggctgctgaa gattctttaa cagaattctt
      601 tgttttatat ccacaggcag aaaaaagtat tacagttccc aacaagcctg cgtaaatccc
      661 tttatgcatg atagctcctc ctaaaataga ttatctttat tcagtatatc acttaaacaa
      721 agaaaagcag atggtttaaa atgaaaaaac cttcccaata aagggaaggt tcatgaagat
      781 aataactttt ttaataatta tagctttcta atatctttca tcaacttttc tagtaatcat
      841 tgggaaaaat ttcaagcagt agatcgcaca aatacctacg acgatataaa tgatcttcgc
      901 aaaaatagtt gttgaaccac cagcaatcat agctactaaa tcaagttcga acaagcctac
      961 caacagccag ttcaaccctc ccacaatcaa taaagttaat gcaatagcat ctaaagtttt
     1021 cattagaatt ctcctttctt aaatgataaa acaattatat atcaaaaaga gaaacattgc
     1081 ttatataacg ctcaggaatt ttagcaatct atgcaaaaag atgaaaattt tttttgctct
     1141 tccattagta cacatttttt agctgaagct gtaaatttca tgtcttaatt ttttttagtg
     1201 agcttttata ctggatttgt aagaaaaaag aaggagagga tcacatatgt cggattggtt
     1261 ggatattgca ggaaaagtag taattgtaac tggaggttcc tcaggaattg gaagaagtat
     1321 cgtcgaaaat ttgttgaagc agaatgcaca agtagcgaat tttgatgtaa ctgaatgccg
     1381 tatacagcat gaaaacctac tatctttaaa agtcgatgtg tcttctaaaa cggacataga
     1441 agaagggata tataaagtaa tgaaacattt caagacgatc gatggtctag tcaataatgc
     1501 aggaataaat attccttctc ttctaataga tagaaatcat ccaaaaagca agtatgaatt
     1561 atcagaacaa gtttttgata agatgatcgc tgtcaatcaa aaaagtgtat atttgatgag
     1621 ccaagcagtt ggaagaatct tggtgcaaaa gggatcagga gtgatcgtga atctttcttc
     1681 tgaatcagga ttggaaggat cagaaggaca gagctgttat gctgctacaa aagcagcgat
     1741 gaacagtttt actcgttctt gggcaaaaga gttaggaaag aggaatgttc gtgtggtagg
     1801 tgttgcacca gggatactgg aagaaaccgg tttgagaaca caagaatatg aagaagctct
     1861 ttcttatacg agaggtataa gcgtcgaaca gctgagaaac ggttacagtc gaacaagtac
     1921 gatccctcta ggaaggagtg gtaagctgca agaagtggcg gatctagtct gctattatct
     1981 ttcagaaaga tcaagttata tcactggagt cacgactaat atttcaggag gtaaaacaag
     2041 aggataaagg atagggggga cgcacatgac gctagtaaac agatggtacc aattattgaa
     2101 attactagtc actcacaaga aagtttcaat ccaggaagtg caaaaagaaa tcaaaaccag
     2161 taatcaaacc ataaaaaaag atattgggga gttgaatgaa gaaattgccg gtattgcaaa
     2221 aatcatccaa aaaaacaaac attttgaact tgaaatttat gattttgatt tttttgatga
     2281 gatcatgcaa ggaaaattga aaacagccag tgatttcaat tcggcatcca aaagaatcgc
     2341 ttatttacta aataggctga tcgaaacaga ggatttctta cgtatggatg atttgtctga
     2401 agaagtcggt gtcagcagag gaacagtggt caaggatcta aagacattaa aagagatgat
     2461 caatgatttt gatgtaaaga ttacaggcac accaaataaa ggactaagaa taataggaga
     2521 tgagctagaa ttacgactac ttttatttta ttttgtttct ccctactttt cagagacgaa
     2581 aacagaacag tttctagaag aaaaacaagc aatcaaacat tttcagaaga agacgaatgc
     2641 tagtaaacaa gaaatcaccc ttttaacgaa agttatcggt atttctttga atagaatctc
     2701 tagtaactat ctgttgaaga agcccatcaa tcgttacagt gggtgttttg aatatatcca
     2761 agaattcaat gaactgatca atcagttgga agaaatctat gaactaactt tgagccaata
     2821 tgaaaaagat tttatcagtt ttcctttgaa catcatcaac acaagtataa cgatgataag
     2881 gaaagctaac tctgatttgc gcttatattt cgaccccatg atgatgcgta ttcgacgatt
     2941 agatctcatt gatctggatg agtccttctt atatcaagag atgaaacagc acctctcttt
     3001 attgataaat cgggtaattt ttcgttatcg attgactgat ttattttatg gtgagataga
     3061 aaaaaagtat cctttttctt accaaatcgc tagcgaatgc atacaagcat tagaagctat
     3121 cttgcaacat aagatctcta aagtagagat cagctaccta acgttgtatt tggagttagc
     3181 atttcgatat caaaaagagc atttttcctc taaaaaagta gccattgttc atacgacagg
     3241 aaaaggaaca gcattaatga tccaaagaca aatcaaaagg atattgggga gggatataca
     3301 aataacacat gtttcagaag aacaatatga aaaagtagat ctaaacaaat atttcgctgt
     3361 ttttacaacg attccgttaa aaaatgtaaa tgaagagaca ccggtgatcc atttgactaa
     3421 tctttttgat gactcttggt taagggcaca atggcaaaaa gcaaaaaaaa agcgattagc
     3481 cgggtttcat tctactgttt ttttctatca aatatggaaa gaaaaaaaga cgtatcaaga
     3541 atatttaaac aaattagtcg ctcctttgat cagaggctat tatgtggatg aagaatttga
     3601 aaaaaggatc ttgaaaagag aatatcagca aagcacattt gttaacaaag aatttgcttt
     3661 tcctcacaca accaaccagc tatctcaaca cattgtttta tcagtagggc taataactga
     3721 tcaaggttta gcagaaacag gaggaaatgg agttattttt ttatttgcca tacctgaaga
     3781 aatgacggct gaagttgaga atgaattatt tgagctatac gatatcctat ttgaactcat
     3841 aaaaatggga atagatgagt ttattgaatt agcaccgaca caagaatcat ttttaaaact
     3901 aatcaaaagt aaaggagaga gacttcatga atgatatgta tctattcata gttttcgcag
     3961 cttcagctta tgtgctgcaa gcagttatca gttggaagca actaaagcat ttcaatcaaa
     4021 catatagcga actgcgaaga aaaggaaaag tagcgattgg taaacgacct gggaaaatcc
     4081 gttcaggcac gattgttatg ctagctatag acgatcatga tcaagtttta gaagtcagaa
     4141 aaatgcaagg ggttacatct ttggcaaaat ttcattcatt acctgtctat gcgggtgaag
     4201 atatccatta catcgatcgt taccatccct cagttcgagc ggaagatcgt ttgacacaag
     4261 cagctttaga aaatgccaga gaagtatact tacgaataga ggcaggaaat gaaaaaagtg
     4321 aacaaccttc attgcctttt attgctatgc tcaataattt tcggatatgg aaaagtacga
     4381 tcaaagaaaa aaggagtgac aaacgatgga tatcataaca aaatttgctg agggatttat
     4441 gaggctgttc caaacaggtg cagaaacatt cataagctgg atgacaggaa tcgtaccagt
     4501 tgtcttgatg ctgatggttg caatgaatac gctgattgct tttttaggtg aagaccgagt
     4561 aacgaaagta gccaaattat cagcgaaaaa tccgcttact cgttaccttg tgttaccgtt
     4621 cttatctgct tttatgttgg gaaatcctat gtcatttaca atggctcgtt tccttccaga
     4681 atactacaaa ccaagctatt atgctgctca agctcaattt tgtcatacga gcaatggtgt
     4741 attcccccat attaatcctg gagaattgtt tgtatggatg gggattgctc aagggatcga
     4801 aaccttaggg ctaaattcga tggagctggc tatccgatac ttgcttgtgg gactgctgat
     4861 gaactttatt ggtggttgga taactgattt tactactgga tttgtatgca gacagcaagg
     4921 aatcgttctt agcaaaaaag tagagctttc agttgattag agaaaggaga gttttaaatg
     4981 tatggtgata ttaagatcgt aagaggaaac ggcgggtatg gaggtccttt gattattagg
     5041 ccgacaaaag ataaacacaa gttcatctat gtcacaggtg gaggtgagag gccaatgatc
     5101 gtagataaaa tagaagaact gacaggtatg gagtctatca atgggttcaa aacatcaata
     5161 cctgatagtg aaatcgcgct tgcaatcgtt gactgtggag ggactcttag atgtggaatt
     5221 tatcctaaaa aagggatacc aaccatcaat atcgtttcca caggaaaaag tggcccatta
     5281 gcacaatata tcactgaaga tatttatgtt tcagcagtag ggttagaaca aatacagcca
     5341 ctaatggaaa ctgatgataa caaagaaaca gtaaatactg catctccaga taaaaagtac
     5401 aaatatgata cttctaaaaa gataaccgag caaaaagcag agcagcaaaa ctggatagca
     5461 aaaattggaa tgggcgcggg aaaagtagtt gctacattca atcaatcagc aagagaagca
     5521 gtagatacga tgatccggac agtgatccct tttatggctt tcgtttccct tttgattggt
     5581 gtgatacaag gttctgggat cggtaatgta tttgctaaca ttatggcacc tttagcagga
     5641 aatgcagttg gattaacaat cattggcttt atttgttcac tgccattttt atctcctttg
     5701 cttggtccag gggcagttat tagccaagtg attggaacat tgatcggtgt agagattggg
     5761 aaaggaaata ttcctcccca attagcatta ccggcactat ttgctatcaa tactcaaaat
     5821 gcatgtgatt ttattccagt cggtctaggt ttggaagaag caaaagctga gacagtagaa
     5881 gtaggtgtac catcagttct ttattcaaga tttcttaacg gcatcccacg agttatcatt
     5941 gcttgggtag caagttttgg attatatagc tg
//
LOCUS       DAZULG010000136         5967 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000136 DAZULG010000000
VERSION     DAZULG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.140"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..1176)
                     /gene="xerS"
                     /locus_tag="ACG9CY_002532"
     CDS             complement(91..1176)
                     /gene="xerS"
                     /locus_tag="ACG9CY_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS4938991.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1525..1720
                     /locus_tag="ACG9CY_002533"
                     /pseudo
     CDS             1525..1720
                     /locus_tag="ACG9CY_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3252..3770)
                     /locus_tag="ACG9CY_002534"
     CDS             complement(3252..3770)
                     /locus_tag="ACG9CY_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS4938992.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4137..4385
                     /locus_tag="ACG9CY_002535"
     CDS             4137..4385
                     /locus_tag="ACG9CY_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4938993.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            4888..>5967
                     /locus_tag="ACG9CY_002536"
     CDS             join(4888..5164,5164..>5967)
                     /locus_tag="ACG9CY_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4938994.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSSSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT         2014 a          982 c          950 g         2021 t
ORIGIN      
        1 tataaaaatg ataatgaagc ttgcctttaa agttgggctt ttatctaatt ttagagacag
       61 gcttcattat tatactattt tttactacat ttacaaagcg ttcaaagcat caattgtagc
      121 tgcattgtct atatgagtat ataaagttgt tgtatttgta cttgtatgtc ccaattgttg
      181 ggcagttaat accaatgaat ttgtctgttg atagagtcta gtagccaaag tatgacgtaa
      241 tttatgtgga ctgacttgga ctctgaatcc ttttgaatat tttgctacca tccgttcgat
      301 tgcaataccg gatatacgct tggcttctcc ttgataaatt gctagaaata aagctttttc
      361 gttttctgtt actttgtatc gttctttccg aatagctaga taattagcta aatattccat
      421 tgcaaatgct gctatattta ctgcgtctct cttatttcct tttctagtta ctaccacatt
      481 attcttttct aaagataggt cctctaaatt aatatttaca acctcggaga gtcttaatcc
      541 agatcctaaa atcaatgcat tgatcgccag gtctctttct acatctcgtt gataatagat
      601 caaagctcgt ttgctcaaag tttttttata ttcattggca atataatcta gataattttg
      661 tgtatcttcg tctagaaata attttttctc gattgcgttt gcacgggcag ctaacgtagt
      721 tttatctgtg atagttgcta cttgatccat aggattattt tctaagtatg gttttcctaa
      781 aggattattg gaacttttcg aaagatattt aaatagaacc tttaatgctg tgatatttct
      841 attgatagta gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt
      901 ctcttttttt acattttcct ttgtgcgacg tttcaaatga gatttgtaca agtttagatc
      961 ataggcagtt aactgtctta tcgaggtgat tgaagtatcg ggaacgatag actcactcag
     1021 aagccattca taaaagagct tataattttt tagatattct aaagtagttc gaggtgattg
     1081 atctttatcc tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat
     1141 tgtatctatt ttttgatata tttctgtatt tttcatgtta ttcatccttt cgataagtag
     1201 gggagaggag tatctctata attggactat accaattata gagtgttttg tcaattggta
     1261 tactctgaaa tttattaatt tatttcaaaa aagcagataa ataggtatag atttaataat
     1321 ttaaaataaa tggtatacat gaataggaaa ggaaacagaa ggaggcaatc ttaatttttt
     1381 aaattgtcta aattatatag aaagaacaat ctttctatat aattagacat aagagagact
     1441 acggttgatt tgatattaac aagcgtatat gaatgtatat acaaataaaa aaggggaggg
     1501 agaagttaaa gaaaattcag agaagtgggg cactcagtag tattaacaat ttaaaaggaa
     1561 tttcttaaag aaaatgggtt gcatcctgga gatacaattt ttttagataa agaaaaaatt
     1621 caagcaacaa ttcaaaaaaa tcttaactgc tgagattgat gtagcaatat ctcaatcatc
     1681 aacaaagtac gatcctgcct ttcaaaagct tattgataaa tgtttattca ttataaaaat
     1741 aaaagaaaaa tttatgtcag cttttttcat caaagtggta atgataattc gtctattaat
     1801 tgtatttttt aggaaatatg gtataattgt tttaacgaaa aggtaattca tgatacacca
     1861 aaaaccacta gctggcgggc tagtggtttt cttttacgtt ctattcagat taacgggtag
     1921 gcttactgtc ttttttggca ataattttgc aatcaagcca tttacagatg taatgcgcgg
     1981 atacttctgc taagattgca aaaaacatat cgaaaaggta attcataata caacctcctc
     2041 cccttgccat aataggccgg ggtagcagac agcataaata ttataccata actgaaaatt
     2101 tcattttagt acatttcaat atattgatga accagcacaa taaaacatgc catgactaac
     2161 agaaaaaaac ttattaaacc agatataact tgtaaacatc caactatata gaaaacgtaa
     2221 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     2281 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     2341 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     2401 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     2461 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     2521 cttatgaaat gaaagacctt ttacatcttg cagaaatcgt tactgggatt aaactaagca
     2581 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     2641 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     2701 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     2761 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     2821 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     2881 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     2941 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3001 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3061 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     3121 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     3181 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     3241 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     3301 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     3361 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     3421 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     3481 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     3541 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     3601 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     3661 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     3721 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     3781 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     3841 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     3901 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     3961 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4021 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4081 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     4141 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     4201 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     4261 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     4321 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     4381 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     4441 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     4501 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     4561 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     4621 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     4681 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     4741 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     4801 ccccacttag cttggatagg ctacaattaa ctagacagaa aaattaaggt gtgtagacta
     4861 gaagaaaaca taccaggagg aatttttatg tctaagagaa cacgaagaac tttttcacaa
     4921 gaattcaagc aacaaatcgt caatctttac ttagctggaa agccacgtgt agaaatcatt
     4981 cgagaatatg aactaacggc ttcagcattt gacaaatggg taaagcaatc taaaacgagt
     5041 agttcattca aagaaaaaga taatcttacg cctgaacaaa aagaattgtt agaactacgt
     5101 aaaagaaacc agcaattaga aatggaaaat gatattttaa agcaagcagc gctgatattc
     5161 ggacgaagag acaagtaatc gatgcgaata agcatcttta ccctatatca gcgatgtgca
     5221 gaatattagg tctatcacgt cagtcctatt attatcaatc aaaaccaaag aaagacgaat
     5281 cagaacttga agaagtagtc gctgaagaat ttatccgcag ccgaaaggcc tacggctcaa
     5341 gaaaaataaa aaaagcctta tcaaaacgag gcattcagat cagccgacga aaaattagta
     5401 gaatcatgaa aaatagagga ttaaaatcga gctatactgt tgcttatttt aaagtacatc
     5461 attctacttg caatgaagcc aaaacgacaa acgtattgaa tcgtaaattc ttaagagaca
     5521 acccattaga agcgatcgta acagacttga cttatgtacg agtcgggaaa aaatggaatt
     5581 atgtctgttt cattttggat ctgttcaatc gagaaattct cggctattct tgtggagaac
     5641 ataaagatgc cgttctagta aaaaaagcat ttagccgtat caaacaacct ctgacagagg
     5701 ttgagatttt tcatactgat cgtggaaaag agtttgataa ccaagctatt gatgaattat
     5761 taacaacttt tgacatcaat cgatcattga gtcataaagg ctgtcctttt gataatgccg
     5821 tagctgaatc aacttataag tcgttgaaag tagaatttgt ctatcaatac acatttgaaa
     5881 ccttacaaca attggatttg gagttatttg actatgtcaa ttggtggaac caccttcggt
     5941 tgcacggtac acttggctac gagacac
//
LOCUS       DAZULG010000137         5811 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000137 DAZULG010000000
VERSION     DAZULG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.141"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACG9CY_002537"
     CDS             complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACG9CY_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS4938995.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2240..4426)
                     /locus_tag="ACG9CY_002538"
     CDS             complement(2240..4426)
                     /locus_tag="ACG9CY_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4938996.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACG9CY_002539"
     CDS             complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACG9CY_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS4938997.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4648..5088)
                     /locus_tag="ACG9CY_002540"
     CDS             complement(4648..5088)
                     /locus_tag="ACG9CY_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS4938998.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5162..5701)
                     /locus_tag="ACG9CY_002541"
     CDS             complement(5162..5701)
                     /locus_tag="ACG9CY_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4938999.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1505 a         1305 c         1015 g         1986 t
ORIGIN      
        1 tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt tttcattatt atctgatttt
       61 caatgtcaaa gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac
      121 tgctggactt aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag
      181 aatattgtaa cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc
      241 tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga
      301 atcgattgcg atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact
      361 tggcgcatca ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt
      421 ctggacaaca gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa
      481 ataatctgct actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat
      541 ttttctttct ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa
      601 agcaacgatt gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc
      661 ttggtaaggt ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc
      721 attaactatt ttcacttgtt gttttttgac tgttgcctct agcccgactc ctgaaagatt
      781 ttttagattt tcagcttgat aaggcttgat ttcatgttct ttcaaataat tcataatgcc
      841 aacagccaat ggatgattgg cattcgcttc caaggctcct agatattgaa ggatttcatt
      901 tttattgaac gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag
      961 tgttcctgtt ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg
     1021 gtttttcaaa agtaatccgt ttttagctgc tattgatgtg gaacgagcaa caactaatgg
     1081 gatagctaaa cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa
     1141 tgcattagga agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa
     1201 tgcgacataa aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc
     1261 tccttgtgct tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc
     1321 agttacttcg atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt
     1381 ggcttgtttt ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc
     1441 acctttcaag attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc
     1501 accttcgttt acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt
     1561 cactgattct ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc
     1621 attcatttca atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa
     1681 gaaatccatg acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata
     1741 aatatatgca actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt
     1801 caattccatc ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc
     1861 taagatcaat acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc
     1921 catcattggt gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg
     1981 tttgaaattc cccatgtgca tggaatgatc catgccgccg tgttcgtgat ccatgtgcat
     2041 cgaatggtcc atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga
     2101 atgatccatc tgactatggt catgtttcat ctccatctgt ttttcatgag attctgtact
     2161 ttctttgttt actacttctt ttttatgctc atgttttttc tccatatcgt tcccattcat
     2221 ttttactcca tcccttctct tactttttca tgtgtcgatt taagcacagg gagttcaaca
     2281 atacactgac ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta
     2341 gtgcggcaaa aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga
     2401 tttttctcaa tgtcaaccta gacaactcga ttgtttgtgc aatggatgcc aaactgctat
     2461 tcatcagcgt cacatcggct gtttccatcg caatgtccgt tccacttccc attgccatac
     2521 cgacatcagc taatgctaag gccggtgcat cattgattcc atcgcctgcc ataccgactt
     2581 ttttgccatc tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag
     2641 caaagatatg ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt
     2701 tgtctcctgt cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt
     2761 tgacttctag tttgatttga tcagccacgg caatcaaccc gatcacttgc ttttcatcag
     2821 ctaaaaacat gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat
     2881 caaatgatag gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt
     2941 taatcgtacc gctgattcca gcacctgggt gggctgtaaa atcaatcata tcgtatgttg
     3001 ctgcttcttg cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt
     3061 gttctaaagt ataaaaaata gataggactt ctttagaacc gactagatct gtaactttgg
     3121 gctttccttc tgtaatcgtt ccagttttat ccaaaacaat gctgtctaag tgagcagctg
     3181 cttctaatgc ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta
     3241 ccataatagc tgttggagtc gctaaaccta atgcacatgg acacgcaatg actaatacag
     3301 agacactatg gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg
     3361 tgataaacgc gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt
     3421 tttggattgg ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata
     3481 ctgtctgctt gccgacttgt gtaacttcca tgtgaagtag accattcgta ttgattgtcc
     3541 cgccaaagag cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac
     3601 tttcatctat cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag
     3661 gacggatgac tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc
     3721 cgtttcttat cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac
     3781 ctgttttcgt ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca
     3841 tactgctgct ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc
     3901 tcaatgcaaa ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg
     3961 tcttgattgc atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag
     4021 cagacagcac taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc
     4081 ctaacatcat agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa
     4141 gatcgaatag catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg
     4201 cttcatcgta caaaattgca ccatagccga tgttttctac gctttggatt aatttttcag
     4261 tggtcgtccc ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga
     4321 ccacaccagg ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca
     4381 tacctgtaat gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc
     4441 tttataaccc aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc
     4501 aaacttgacc gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag
     4561 tgcgcttact gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga
     4621 aaattgttgt ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg
     4681 gatacagtta cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc
     4741 attgatatct tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat
     4801 cctttttgca cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc
     4861 cgagacagct ggataataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt
     4921 ttttaccaaa cgacccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc
     4981 taagatctgc gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat
     5041 ctcccactct gaatcactga tctttactgg cgttttgact ttttccattt atttcacacc
     5101 tttcgtttac aaatgtaatc attatcttat gaatacagat tacacttgta aacaaaaact
     5161 gtcaattatt tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg
     5221 ttttaaggat gatcagttta tttcttgcat tagctacttg gatcagctgc tgtattttcg
     5281 gttcattgtt ttcttcaaaa tgccagataa aagataaaaa ttctaagtat tccctgttta
     5341 gtttctccga aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta
     5401 gagaacgcca aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct
     5461 tatctgcttc tttaatcctt tcctctaatg tagaagtata atttccttca acgatccatg
     5521 acggattaga taccataaac aattgctgtt cttgcaaaaa ccactttttt gcctgaatac
     5581 tgtagtctgt tgtatgccat aaaaggtcta gcggcagtag cggaaagtcg gtgatttctt
     5641 tgatcttcct tgcgagggtc gattttcccg acccactttg tccaatcacg atgatcttca
     5701 ttcttctccc tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa
     5761 aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaata a
//
LOCUS       DAZULG010000138         5759 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000138 DAZULG010000000
VERSION     DAZULG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.142"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG9CY_002542"
     CDS             complement(<1..300)
                     /locus_tag="ACG9CY_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS4939000.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG9CY_002543"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG9CY_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG9CY_002544"
     CDS             complement(1014..1304)
                     /locus_tag="ACG9CY_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939001.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG9CY_002545"
     CDS             1706..1942
                     /locus_tag="ACG9CY_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939002.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG9CY_002546"
     CDS             1949..3901
                     /locus_tag="ACG9CY_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4939003.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG9CY_002547"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG9CY_002547"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4939004.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACG9CY_002548"
     CDS             complement(4797..5489)
                     /locus_tag="ACG9CY_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4939005.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1887 a          994 c          916 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatt
//
LOCUS       DAZULG010000139         5716 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000139 DAZULG010000000
VERSION     DAZULG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.143"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..168)
                     /locus_tag="ACG9CY_002549"
     CDS             complement(<1..168)
                     /locus_tag="ACG9CY_002549"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4939006.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFV"
     gene            complement(162..563)
                     /locus_tag="ACG9CY_002550"
     CDS             complement(162..563)
                     /locus_tag="ACG9CY_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4939007.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(750..2558)
                     /gene="uidA"
                     /locus_tag="ACG9CY_002551"
     CDS             complement(750..2558)
                     /gene="uidA"
                     /locus_tag="ACG9CY_002551"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS4939008.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2581..4347)
                     /locus_tag="ACG9CY_002552"
     CDS             complement(2581..4347)
                     /locus_tag="ACG9CY_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFS4939009.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(4360..5154)
                     /locus_tag="ACG9CY_002553"
     CDS             complement(4360..5154)
                     /locus_tag="ACG9CY_002553"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4939010.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(5164..>5716)
                     /locus_tag="ACG9CY_002554"
     CDS             complement(5164..>5716)
                     /locus_tag="ACG9CY_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4939011.1"
                     /translation="VLMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYL
                     ILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVY
                     SFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLIL
                     FFLYPLSKDKVLENCELLKQKHK"
BASE COUNT         1829 a         1133 c          695 g         2059 t
ORIGIN      
        1 aacgaattga cctacctcgt taaatccatt tgtcaaacat aacttagcca tataagaatg
       61 ataatgtttg agtatcatct tgatcgaaat tggatctcct tctagagcag ataagataat
      121 ggatagtgga actaataggt actgccgctt tttagattta tctaccatag gtcatacccc
      181 attttcaatt gttcaactgc cttctttcgt tgataccaga taccacttgt gctcatattg
      241 agaattttac cgatttcagc atcattaaaa cctgcaaaat aataaagtag gataatcttt
      301 cttcttatct ccgataactg atcaattgct gctgataact tttgatctga aattaatatt
      361 tccattccaa ataagaaata gacatcttgt tcatcaaaat gagtttggga atacctgcct
      421 tcatttgaat ctgccaatgg ttcctcatta tcctttatgt tagcgtttcg ttttcgaata
      481 gacttagctt cgtttcgtaa tactttttta cagtaggcat caaattggtg ttgtatagat
      541 atgtcattgt aatgttgttg catgataata tgctcctttt tccccctttc cactaataca
      601 acaatcagaa agcccctttt tttcgaaaaa actagaaaaa aaacataaaa atatttatca
      661 ttctttaaac agaaaatctt tctataaaaa acaaaaaaaa acgattaggc taggctaatc
      721 gttttttgaa cattcttaaa aaatccaaat tattttttat aactgaaatt gggtattgat
      781 gaccatcgtt tctgtagata gtaagcaatc attttaggtt ttctatctct agtaaagatt
      841 cctttcttat ttccttgcac tcttaaaatc ccttgacttg ttgcaaaatc tgcaaaattc
      901 cacacttgtt caccaacaaa attttcacat gtatctatta cttcgtgatt tgcttcgtaa
      961 taagccactt gatactcttc tgtaaacatt gttggtactg tatcatgaaa tccagctact
     1021 gtatctgctc catattctgt aaacattatt ggcgtatcag gacatctttc catccatcca
     1081 tctagctctt ttttcaacag atattttgca tgtgccagat cccctcccga aacataccaa
     1141 ccataataac gatttaaaca taaaacatcg acaatatctc ctaccttaca aacatctggc
     1201 gttgatttta gataagtaac gattgttaca ggtcttttct gcggatcaag ttctttagtt
     1261 aattcaacta aaggttcaaa atattcttta gcaccttcgg aatccgaatc tggttcattt
     1321 gcaatagacc acattacaac acttgggtgg ttcttgtcgc gttctatcaa ttccttgatc
     1381 accgttttat gagaattttc tgttcgtatc tctttccacg tattatgttt ttccacatta
     1441 tcatctaata aagtggccat gaaatttaaa tgtaacccta ctgcaggtac ttcatcaata
     1501 actacaatac cttctcgatc tgctaatcgc ataatttctt ctgagtaagg ataatgtgat
     1561 gttctaaaag aattagcacc tatccatttc attaaattaa aatctttaat atttacagcc
     1621 tcattcaaac cacgtccatt aacatacgaa tcctcatgct taccaaatcc tttaaaataa
     1681 aaaggtttat tattaatcaa aaatttgcca ttggtaacct gaacactgcg aataccaaat
     1741 tcttctgtat aggtatcgat cactttatta tcatcaatta aactaatttt catactatat
     1801 aaataagcgt ctagaggttc ccaaaaaatt ggattcttaa gctgaatcat tcctttcttt
     1861 ccttcagctt cagctacaag ttgagcctct tcgtcatata tcgctattaa gatttttccg
     1921 gtccattctg tatcgatatt gaattctaca cttgcatcca aatcagttat tcttgtatta
     1981 atagtaatat cacttatata gtctttcgga gttgtatata acttaacagg tctatgaata
     2041 cctgcataat tgaagaaatc aaaattaact aagttttcct tttttatatc cccattttcc
     2101 tgtactgttt ccttcataat ccccacaggt aaagtactat cgtcaataat attgtttaca
     2161 gcaactgtaa ttcgattatt cccgtctttt acaaaccgat taatttcaga ctcaaatggt
     2221 gtaaacccac ctttgtgttg agtaataaac tttccattca aaaaaacttt tgcttcatgt
     2281 gttgcagatc caaaacgaag aactaatctt tcttctaata atcttttagg aagagtcact
     2341 tctttttcat accacaccca tccaacatga tttctaatgt tctctgtttc aaataaatca
     2401 ttataagaag agggaactga aatcggataa ctctcttttt catttattgg ttctaaagca
     2461 agttcttcct gtaagtcatc atttgtatct aatttaaatc tccagagacc gcttaagtca
     2521 attacttgcc tcgtctctgt cataatggga tacaacatat aaaccactcc tatctaaata
     2581 ttaaataaat gtatccttta aaccacaata agcatctatt ggacttactc ccttaaattc
     2641 agaaagaccc attagtttgt ccaccaatat ttctaaagtt tcttgttttc catcataagt
     2701 attaatataa gtctttactt gaggtacatc agccaaatga aacggacact gtacagatac
     2761 aaaaattgtt ggaatttcat gaatgtaaaa cggtatatcc ggagttcctt tacttgctgg
     2821 ccagacaatt cgttgaactg tgcctggatt aacattagct atattaatga tcaagtcata
     2881 ttttttagtt aaatttttaa ttggtctctt agcagcatat attccagcta tatttttaat
     2941 tttttcttct tgcgataatc ctttcaattc ttcttcaggt gaaatgtatt tttcaacttc
     3001 aaatcctttt gctgacaaaa tctgtttcaa taaatcaatg gcttgaggtc tatttccagc
     3061 aactacttca ccaaacccac cttttacccc atgtacatct actagtaaaa tccttttata
     3121 tttttctggt acaataggcc aaatatcttg tttatcttta actaatgtaa ttgcttggtc
     3181 agccacctct ttaaaaattg atttattttc aattaaacca attttttcca gagcttcttg
     3241 cttaggtaat aaaatttgtt cttttgcttt tttgtgtaat cccaatttag cttttaaccc
     3301 taaaattctt tttaaagcat ctaataaccg atcatcagtg ataattcctt tacgatagcc
     3361 ttccatcata taattaaagt cttcatctgg atcattaaaa aatagaaata gatcacatcc
     3421 agctgcaata gaagtgggca ataattctga tcgtttcatt gccccagtca ttcctaacat
     3481 atgactagca tcagtaacta ctaatccatt aaatccgagt tttcctctaa gtaagtcagt
     3541 gattaattct tttgataaag ttgcaggaag taaatcttca tctttcagat caggattaaa
     3601 atgttttgaa taagctggca tatggatatg acctgccata attgaaggta atccagcttc
     3661 tattaagcct gagtatactt ttccaaaaga attatcccat tcttccgtac ctaaactatt
     3721 aatactaaaa gatagatgct gatctcgttc gtcaataccg tcaccaggga aatgtttagc
     3781 tgcaggcaaa attccactct cctgaatacc tttcatataa gctaaactaa gttctaaaac
     3841 tttatcaggg ttttgtccaa aagatcgact agaaatgata ggatttctcc aatttctatt
     3901 aatatctaca ataggagcaa aactccagtt acatccaata gccgatgctt caatcccaga
     3961 aactctccct aattcataag catatttcgc atcatttgtt gcaccaattt ttacttctac
     4021 accaacttca gtaccatctg tacaagctcc gttaccacct gcttccgtat tagcagcaat
     4081 taatagagga attttacttt ctgtttgtaa gatatagttt tgatcatata cctcatcagc
     4141 agttcctgga ttatatcgta cagctccaat atggtactga ttgaccatat tactcaagta
     4201 atttttatca cgactagatc ccatattaac aaacaattga cctatttttt cttccaaagt
     4261 catagaatca atcgtctctt caatccaaga aatagcatct tcatctaaat tatatggttt
     4321 ttttcgtaaa tctactttta tagtcatttt ctttctcctc tacttattag taagattttt
     4381 ccaaaaagta tctatatatt tttgttcaat atctacataa tctcccgata aacatttaaa
     4441 taaaacatca gttcttaagt tttcccatga ttctttttct ttattgacca aaataggata
     4501 aaagaaaact ttatttttat ttgcagcttc tagatcacca ggagaatcac caatcattaa
     4561 tatcttatcc aaatcacaac cattttcaat tacacttcga ataatatctt ctttttttcc
     4621 tttatcttga caacacagtt catcaactaa tgataataat tcatgtcttt cccattcttc
     4681 ttgaacagct tccttattag ctgagcttac aataattatc ttcccatatt ttttcagagc
     4741 ttttatagta tctctagcac ttaaaaaggg tttatctaat ccttttgctt gactaatctg
     4801 ttgattgact tcttccgacc attttaaagc ttttaataaa tcctctgact gagaatattc
     4861 tatttccctc ttcaaagatg gattagacaa ttcttttgtt agagataccc agtgctttaa
     4921 tcgattaatc gatgttacat catttttttc taatgtttta actaaaccta aaaatctgtt
     4981 tattccacga gtctctgaat ataaattaat attattccaa tcctgtagga atttttctct
     5041 atgatgaaca tcaaaaatct ctgcagcaat tggtccgaaa aagagattgt gtttataagt
     5101 catcgtatcc ataacacagc catctgaatc aatacaaaat atccaattat tcatatttct
     5161 ctcctattta tgtttttgtt ttaaaagctc gcaattttct aagactttat ctttagataa
     5221 tggataaagg aaaaataaaa ttaatcctac aatcagaaaa caaattgatg gaattaatgt
     5281 tgaagcgtta tataactttt ctaaaacatc tcccgtttgt acatttgcta aagagtcata
     5341 gcctataata gaaagagtcc aacctccgac gcctccagct agagcttgac ctatttttct
     5401 agcaaaagaa tataccgcat atacggtccc atcttctctt tgatcagact ttacttcttg
     5461 atcatcaata acatcagtaa tatttgccca aataacagta ttaaacacac ccaaacccat
     5521 atagccaacc gaactaagaa ttaagaatac atacatatta ctagctttta tcaaaaataa
     5581 gatcaaatat actattgctg aaaagaacat tcctactgat gccagttctt tttttccata
     5641 ttttttactt aaaaacaaag ttaaaggcga aacaactatt aaaaccaaaa taggattgat
     5701 aaaattcatt aaaact
//
LOCUS       DAZULG010000140         5675 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000140 DAZULG010000000
VERSION     DAZULG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.144"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            664..2487
                     /locus_tag="ACG9CY_002555"
     CDS             664..2487
                     /locus_tag="ACG9CY_002555"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4939012.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            2501..3910
                     /locus_tag="ACG9CY_002556"
     CDS             2501..3910
                     /locus_tag="ACG9CY_002556"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4939013.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            3928..4764
                     /locus_tag="ACG9CY_002557"
     CDS             3928..4764
                     /locus_tag="ACG9CY_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4939014.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            4829..5611
                     /locus_tag="ACG9CY_002558"
     CDS             4829..5611
                     /locus_tag="ACG9CY_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939015.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
BASE COUNT         1779 a          932 c         1233 g         1731 t
ORIGIN      
        1 aaaattatta tggatatgtt tggtattttt attgataatt ggtggaattg aaatacttac
       61 tacatgtaga ttagaaataa atcagtttaa acatttgcta gttaattttt aagaatacta
      121 gcccaaatca ttctatcgat acctttatat ctcggaatga ttgaaattgg ctattgctta
      181 ctgattttta ccaaaaaaac aagtatcact attgttgcct atattgttgg aatgctgttt
      241 attccatcct ttactcacca aattcatcaa tttttcccaa gagcagaatt gttgaagttg
      301 tgtgatccgt tatatgcatt tttgatgctt tcacgatttt gggaatttcc cgctggttat
      361 gtaggaatgg ttttactggt atagttaata ttagatatac ttattttagc agtaagtata
      421 cgcagatata ccctagttga tgtgatctaa ctgttatatt accctatacg gtcaaacgaa
      481 aagataaaaa attaaattcc ctgttgattg cgctatcata atgtgctatt attggattac
      541 agcaaaataa cgttattggc atgttactga ttatgcaggc aagaccaaga caaactaaca
      601 cttcaatgtt gattggggcg tggtttgact tggtcttttt tactgcttta aggagagaga
      661 ataatggatt acagagaagt aggaagaaat gtattgcaac aggttggggg aaaagaaaat
      721 gtggtcagct taacccattg tgccaccaga ctgcgttttg aactcaaaga taaaaggaaa
      781 gccgatacga aagccttaga aaaaacactt ggtgtaatta gtgtggtgga tagcggtgga
      841 caataccaag tggtgattgg caatgaagtc caaacagcct tcaaggaaat tcaaaaggaa
      901 gtgggaactt caattaaaaa gggcccctct tcagacaatc aagaaagtat tatttcaaga
      961 ttgattagtg tcatttctac cacctttaca ccgatgattc cagctataac aggtgccggg
     1021 atggtaaaag ccattttggc cgtcttgact ttgactggtg tactatcaga cagtagtcaa
     1081 acttacatca ttttgaatac tattgctgat gcagcctttt attttatgcc agtactatta
     1141 gcctatgggg cagccataaa gtttgatgtc aacccaattt tggcgattac tgttgctggc
     1201 attttgcttc atccaaatat ttcacagctt ttttctactg gcgatgccgt ttcttttcta
     1261 ggaattccgg tattagctgc agattatgcg ggatcagttt taccgattat ctttactgtt
     1321 tggatcatgt cctatgtaga gccctttgcg gatcgaattt cgccttcgtt tattaaattc
     1381 tttactaagc cgatgctggt atttttaatt atggggcctt tagcgttggt tgtcattgga
     1441 ccgtttggaa cgttgctgaa cgatcttata gcaatggggg ctgaatggat taacggcaaa
     1501 gcagcttggt taattccatt tttgatgggt ggtttgcagc catttttggt ggttacgggt
     1561 acagcttggg caatgacgcc aatcgctact atgcaattga gcagtacggg ttctgaaatg
     1621 atcaatgggc caggaatgct ggcttctaat attgctcaat caggtgctac ctttgctgta
     1681 gccgcgaaaa ctaaaaacaa agagttgaag cagctagctg tgtccactgg gatcactgct
     1741 ttaatgggaa ttactgagcc ttcactctat ggagtaacct tgaagctgaa aaaaccatta
     1801 gttgcttcca tgattggtgg tgcaattgct ggtttgtatg ctggcttgag cggtttagtc
     1861 agatatgcct ttgtttcccc tggcttagca gccttaccag cctttattgg agaaaatcca
     1921 atgaatattg ttcacgcaat tattacgtgt gtaatcgcct ttgttgcaac ctttatcatt
     1981 gcttatctaa ttggatttga cgatacttct gatgaatcag agcaaacggc aatagccgaa
     2041 gcagtcattg ttgaaaatcc ggtaaaagga accgtgtgtc ctatttctga agtgaacgat
     2101 gctgtctttt ctaagggact attgggaaag ggcgtagcca ttcatccaga agaaaacagc
     2161 atttattcac ctgttgaggg agaagtcatt actattttag aatctaaaca tgcgattggt
     2221 ctacgttcta aagaaggtat tgaattattg atccatgtgg ggatcgacac cgtgaattta
     2281 aaaggggaac cctttgaagt atttgtaaaa gcaggagatc aggtgtcagc aggacaatta
     2341 ttaatcaacg ccgatttcgc tgccattaga aacgctggct acgatgatac agtgattgtg
     2401 gtagtgacca acagtgaaaa ttacgcagga attagtgaag caagcggaag cttaaaggaa
     2461 cacgaaccat tgatgcaaat tagttaagga gaggattttt gtgcaataca aacagctttc
     2521 agcatttcca gagaagtttt tatggggtgc ctccattagt gcctaccagg cagagggtgc
     2581 ctatcaggag gatgggaaac aaccgtctat tattgacaag tatcagcata cagaaggggt
     2641 agctaatttt gaagttgcct cggattttta ccatcactat aaagaagata tacagctgtt
     2701 taaagaatta ggcttaaaag cttttcgctt ttccatcgct tggacgcgaa ttatggatat
     2761 tgaaaataat aaaaccaatc aaaagggaat ttctttttat catcaagtaa ttgatgaatg
     2821 cctgaatcag ggaatcgaac cgattgtcac catgtaccat ttcgatttac cttattttct
     2881 agaggagcag ggcggttggt taaatcgagc aactatcgat tattttgtgg actatgtaca
     2941 ggtattgctt actgagtatg gtgacaaggt gaactattgg ctgacaatta atgagcaaaa
     3001 cacgatgatc cttcatcctg gagcgattgg tttgccacca agtggagagc tgccttcaaa
     3061 aaaagaactc tttcaaatta atcaccatgt tttattagct caagctagag taattcactt
     3121 gtatcatcag ttgaatctta aaggaaaaat cggtccagca atcaatttaa cggctatgta
     3181 tcaagctacc tccagtccag aagatgcaat tgcagcccat aattgggaaa cattgagagg
     3241 ctggtctttc ttagatgtag cagttcgggg aaaatacaat tatttgtttg aaaactattt
     3301 gaatgatcgt ggattatatc ctaaaattga ggcagaggat caggcaattt tatcctccgg
     3361 taaacccgat tttattgcga ttaattatta ttcaactgcc accatcgctg ccagcaaaaa
     3421 tgacggatca gatgtttccg ctagagcggg ggatcaacag atcatgttgg gagaagaagg
     3481 tgtctaccgg gcagctgaaa atccttatgt agataaaacg ccatatggct gggtagttga
     3541 ccctactgga ctacgattaa cattgaggaa attgtacgat cgctatgatt tacctatttt
     3601 aattactgaa aatggctatg gggcacccga catagttgag gaagatagac gaattaatga
     3661 ccaggatcga attgattatc tagccaaaca tattctggct attcaagcag ctttaacaga
     3721 tggtgtagat gtttttggtt atttaccttg gtcagcgatt gatgttgtta gtacccatca
     3781 aggcttcaat aagcgttatg gttttatata tgttgaccga accgatgaga atcttaaaga
     3841 attaaaacga ataaaaaaag aaagcttctt ttggtatcaa aaagtaatcg caagaaatgg
     3901 cttagcttag agagaacggt gaatctaatg agagtaatta aaacgttaaa caacagttta
     3961 attctttgct tagataagca aggcaaagaa gtaattttga tggggaaagg gattggattt
     4021 caactaaaga aaggcgattt ggttccggct gacaaaatcc aaaaagtatt taaattagat
     4081 tcactttctg atgctctaaa aaaggattac attaaactgt ttgaggctgc tccggatgat
     4141 ctaattattt gcgtcaagcg aattatcgat tatgccaacg ccaagtttga ctttaagctg
     4201 aataaaagtc tattttttac cttggttgat cacttgaagc atgccattga acgcagtcaa
     4261 aaaggcattg tttttcaaaa tcgcctgctg attgaaattc aaaagtatta cccaaaagaa
     4321 tttgagatcg gctgctatgc tatccaattg atcaacaaaa aaatgaatgt tcagttgcca
     4381 gaagaggagg ctggaaacat tgcgtttcac attgtaaatg cccaaagtga acaagcaaat
     4441 atggaagaaa ccatgctctc ggtaagagca ctaaaggata tcttggctat gctaggatat
     4501 ctgtttcccg aaaaaaaggt tgataaagat tcgatgcttt acttaagatt tgtgacgcat
     4561 ttacagtttt ttattgcccg aatgattaag aaggaagagt tgccggagaa ggatgatttt
     4621 ttgctggctt cagtgaagca gcgttttccg aaagagtatc gggcggcctt gaaaatcaag
     4681 gagtacgttc aagaagagat gggtgcctca gcaaatcagg acgaactgct ttatctaaca
     4741 ctgcatattt caagggtgta ctgaatgaag aaaatatcgg ctgcacaata tataaagact
     4801 tgttggttgt gctaaggctc ctgattcctt ggaaaattct tctaacagtg cggaggtgaa
     4861 tgtttcgacc tctaccagtg aagtggtaaa gtgttcacaa gaaactacta gcttgccaga
     4921 agagctactt ggagattggc agattaataa taaggatgct ggtatagaga tatcagacag
     4981 taaagctgta ttccgtatag gaaggtctca agtagagtca aaggattatt atgtagatgt
     5041 aaacaacaaa atgcttttac atgtctattt ttcttttgag tatgatagtg tgaaatacat
     5101 tgaagtctct attgaaagaa ctggttatag tgaatttatt agtaaaagcg tgggaatttt
     5161 aactatgcac actaaagatg gacaatatat aagtttgggt gtgaagagag tttccggtga
     5221 gaataatgag acagaagcag ttacggagca gacgatggat gaaagaatcg attcatctaa
     5281 gagtaatgag acagaagtag atactgggaa gacgatagat gaaagaattg attcattcat
     5341 cgaaacatac acagctatac cacctacttg gaagcttgta aatgaagata aaggaatcac
     5401 gataaatact gatacagaga tttctacaga tgctcaatct ttatcagatt tattagctaa
     5461 tctaacaggt atcagtaatg cagtgacaga gcaagttggt aaagacattc cacttcggtt
     5521 acttgaaaat gggaaaaaag attattctgt ggtatttaag aacggacagg taactgatta
     5581 caacgaagta tttaaaaaag ttattgaata atgattcttc catctttttc atttatttac
     5641 cctaccccgt tgttatatga ccgtacttat acatc
//
LOCUS       DAZULG010000141         5608 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000141 DAZULG010000000
VERSION     DAZULG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.145"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1115
                     /locus_tag="ACG9CY_002559"
     CDS             33..1115
                     /locus_tag="ACG9CY_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS4939016.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1115..1918
                     /locus_tag="ACG9CY_002560"
     CDS             1115..1918
                     /locus_tag="ACG9CY_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4939017.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            1988..2728
                     /locus_tag="ACG9CY_002561"
     CDS             1988..2728
                     /locus_tag="ACG9CY_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4939018.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            2739..3743
                     /locus_tag="ACG9CY_002562"
     CDS             2739..3743
                     /locus_tag="ACG9CY_002562"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS4939019.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(3788..>5608)
                     /locus_tag="ACG9CY_002563"
     CDS             complement(3788..>5608)
                     /locus_tag="ACG9CY_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939020.1"
                     /translation="NKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKE
                     IKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWI
                     TVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVY
                     LGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFAL
                     ALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTF
                     QLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPW
                     IVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVD
                     FLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEE
                     AKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHA
                     LEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQER
                     FDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         1804 a          927 c         1140 g         1737 t
ORIGIN      
        1 cacaatatga atatagaaat gggggaaaac atttggatac aaaagaagaa cagctactta
       61 ttgatttaac ttctgcaaaa ggagtacctg gaaatgaaga agaggtaaga gaagtattca
      121 gagaatatgc caaaccattc gctgatgata ttttttatga cggtttaggc agtgtgattg
      181 ccaaacatgg tgctggcgga ggccccaagg tctttatttc tggacatatg gacgaagttg
      241 gttttatggt aacaaaaata accgaaaaag gattcttgga attccaaact cttggaggtt
      301 ggtgggggca agtcatgctt gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg
      361 tccacggtgt gattggttcc aaaccaccac atgttttaac acctcaagta agaaataaac
      421 cttatgaaat aaaggatatg ttcattgata ttggcgcatc aagccaagaa gaagccaaag
      481 agtggggcat acgtcctgga gatatggtca ctccatatat tgaatacaaa cgaatgaatg
      541 gcagtaaata tttactggca aaagcatggg ataatcgtat cgggacagct gtttctctgc
      601 gagtattaga gaatctatct aaagaagcac acccaaatgt tttattcgca ggaagcgatg
      661 tgcaagaaga agtaggcttg cgaggtgctc gaacaagtac acaccttgta aatcccgata
      721 ttgcttttgc tttagatact ggtacagcag gagacactcc aggaatgaca ccaaaagaag
      781 cagattcgat tttaggaaaa ggcccacaga ttttgatttt tgatgcatct atgatcccgc
      841 ataaaaaatt attgaatttt gttattgatg tagcagagga aatggatatt ccattccaat
      901 atacagtgat ttctggcgga ggaactgatg caggacaaat gcatttgaca agagatggtg
      961 ttccgtctgt agccattacc gttcctgttc gttatctgca ttcccataca tcagtgattc
     1021 atgaagatga ttatttgaat acagtgaaac tagtcacaga attagtgaaa cggcttgata
     1081 gtgaaacagt tgaaaaaatt cggagttatg aataatgaat aatagaatac ctatttctga
     1141 atatcggaaa aaagcacgag tttctttgga aaatcaatgg ggaatcaata cttggcttgt
     1201 tttcttatct ataatattta cgagcgtcat ccagaacttt tttgacggtt tcagtccttt
     1261 tccggaagaa agcatgcagc agcacttatt gagttttcta gtcgataatt tacttttatt
     1321 cgcattcaac tatgcattgt tttacattgc gctagtagtt gtacggggtg gcagaccaaa
     1381 aagtaacctt ttattttcag ttttccaaag aaagtatttt ggttcgatac ttggcatcaa
     1441 tttgatcaat tcagtcatca actggctact caacagttta atctttctgc ccatgttttt
     1501 aatcagcgga gtgagtactt atatgcagct gcttttcagt tcaaatgcgg tacctacggg
     1561 tgtcatgcca gaagattttc ttgatatcag ctttgtttta tcgatgatag ggatgatttt
     1621 tgtttcagca ctcatcatga gtattgttgg cggtttattc caatttgctg catggacaaa
     1681 gttcgactat cctgatttgt cggttattca atgtttgaaa tatggctggt ttttattgaa
     1741 agatagaatt ggtacgtata ttttgttgca gctgtctttt atcggctggt atttgctagg
     1801 agcaatcgct ttgttcatcg gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc
     1861 agaattttat gaacaagcac ggattgaaaa aggaggtcca gacgcctatt ttacttagca
     1921 tgtaagcagt ttattcatca aaaaaataac ttttgtggta ggataaatct ttgaggggga
     1981 atgtacgatg aaattaactg ttttaggatg cttaggagcg tatccttata aaaatcaagg
     2041 gacaacaggt tatttgcttc aatctgacgg gttcaatttg ttgatcgatg caggtagtgc
     2101 tacgctgatc aaattacaag aacaccttga tccacttgat ctagatgctg tgatccttag
     2161 ccattatcat catgatcata ttgctgacct tggtgtatta cagtattact ggcaacttca
     2221 tcctaagaga aatccggaga cattgctttc aatttatgga cacacaaaag attccattca
     2281 ctttgaagaa ttgacgttgg aaaatgtctc aaaaggacat ccttattttg aagctgaaga
     2341 attaaaaatt ggtccttttc tgattacttt tatggaaaca atccatccag tagtatgtta
     2401 cgccatgcgt atcgtagaag aaacaacggg gaaagtattc gtatttacag gtgattcagg
     2461 ttatttggaa agtttcgctg attttgcgaa agatgctgat ttgtttctag cagataccta
     2521 tctttttgaa ggaaatgaac atcatcatgc ccatttcact tcaaaagaag ctggagaact
     2581 tgcgaaatca gctaacgtaa agaaacttgt acttacacac ctacctcagt ttggtgattt
     2641 agaacaattg aaaaatgaag cgcaaaaggc agcaggcaaa gggattgata tagaacttgc
     2701 tgcagttgac aaagtgttta acatttagga ggaagaacat gatttttgta ccaaatgaaa
     2761 accacgaccc aagaatcaat ttagcaattg aacaatattt gctgcaagaa atgcctttag
     2821 atgaacctat tttgctcttt tatatcaatg aaccttcaat catcatcggc agaaaccaaa
     2881 atacgcttga agaaatcaat cgagattatg tggaagaaaa tggcattcat gttgtccgtc
     2941 gcttaagtgg aggcggagct gtctaccatg attttggaaa cctgaacttt agttttatca
     3001 tgcctgatga tggggattca ttccgtgact ttgcgaaagt aacacagcca atcatccaag
     3061 cacttcatga attgggtgtt gctggggcgg aattgaaagg ccgaaatgat ctagtcattg
     3121 atggcatgaa attttctggc aacgcgatgt atgctaccaa cggtcggatg tttgctcatg
     3181 gaacaattat gttcgactct gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag
     3241 acaaaatcga atcaaaagga atcaaatcga tccgttctcg tgtaacgaat atcaaaccat
     3301 ttcttccaga agaaaagcaa aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga
     3361 aaattttcgg cgtagagtca gtagaagaag taaaaacgta cgaattgact gatgaagatt
     3421 ggaaaaaaat caatcgaatc tctgaggaat attatcgaaa ttgggattgg aattacggga
     3481 agtctccaga tttcaatttc tctcgccaaa aacgtttttc tatcggttcg attgaagtcc
     3541 atttgaatgt ctcagaagga atgatcaaag aagcaaaaat ctttggcgat ttcttcggac
     3601 taggagatat ctctgacgtg gaaaaaaaat tggttggaca gaaatatgat aaagcatctc
     3661 tatcaaaaat agtcagccaa attgatgtga agaaatattt tggtgcgata gaaccagaag
     3721 aattgcttac cttactttat tgaaaataaa aaatagcgga atccacatat atggattccg
     3781 ctatttttta tacaggattc gggtaatttc gagagagttg aacagtgatc cgatcaatga
     3841 aagtatcttt ttcctgcgtt agtgttcctg tgtaacgatc cgctacgtct ttcatcatca
     3901 ttaagtcaaa acgttcctga tcatctaatt cttctgtgat gcctcgacta aataggtttg
     3961 tatcgtaatg tgcactatgt tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg
     4021 acaaataaat ttttcgatac agtggatttt cgatggcatg agtttctcta aaggaatgct
     4081 ctaaagcgtg gatcaaaagt tgagccagat cgtcatcgtt gataggaagt tcttcgggaa
     4141 gatctgcttg gaaggttatc tgaatattcc gtttggcagc taacttctgg taacgcgcta
     4201 agataagatt tgtcacctgg tggttgcaaa aagaacacag catttcgaaa ttttttgctt
     4261 cttctaatat cttttccaca tagtttttag cttttgtttg gtccgtatgc attaaatctt
     4321 gcagattctc catctcttgg acaaattcca ggcgaagtgt atttacttct tggatatgct
     4381 gtcgaagctg ctcataatgt tgctccaaca aatcattttt gatttttaag aaatctactt
     4441 gtctccgatc tttgtcggta tttaagatgt gatcgattag attgtagctg ataataacga
     4501 ttagaatgat aatatataaa atcgtagacc aattgatcgt tgtttgatag atacctaatg
     4561 ccgtttggat gtaaatcaag caatggataa tggctaccaa gacgatccaa ggtgtacaag
     4621 ctacataaaa acggttattc ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag
     4681 tgcttataac actagcaata ttagcaatcg gcattaattc aggaagttct gcaggagtaa
     4741 aagttgcata aagcagaacg ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt
     4801 accatttttt atagtaaaac attcggctcc aatagtagca gatgatgaaa gctgacgtta
     4861 agataataaa taaatgagat aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta
     4921 aaatatcttc tgatacagct tcaaatccta atgccaaagc aaagcaactt aagagaataa
     4981 gtgagtattc aagacgtttt tgtttataca tcaagtagat cgtatagaaa aaggtcccta
     5041 tagcgataaa aacagcgatt atcatcgtca atacttgcgg cactgattgt gaataaataa
     5101 aactaatcaa tggtccagtt gttcctagat aaactttcgg tggcagtcca gcatagttct
     5161 gataaggaga tgagactgta agggtcaatt tttttccgat ataatttgct ggtaaatcta
     5221 tcacagccaa tgagagtccg ggattgcttt cttcctgata atctttccgt tggtataaaa
     5281 tcttttcgcc tagatataca gtaatccatt gatggtttcc tttgattagt aaatttgctt
     5341 cgtccaacgg tttttcaagt gtcgtttcca taatgaatgt ttcgtcttta tccacggtag
     5401 gaatcgtacg agtgtacttg ctgtcaaact taaaatcagg atcatctttt gtatagaaaa
     5461 cccacgaatc ttttatttct ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa
     5521 agaaataagc aattcctgtt aaaaataaca atgataatat aatgattatg ctccacttta
     5581 tacgtaaata aaaagattct tttttatt
//
LOCUS       DAZULG010000142         5571 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000142 DAZULG010000000
VERSION     DAZULG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.146"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..457
                     /locus_tag="ACG9CY_002564"
     CDS             146..457
                     /locus_tag="ACG9CY_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939021.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            454..1078
                     /locus_tag="ACG9CY_002565"
                     /pseudo
     CDS             454..1078
                     /locus_tag="ACG9CY_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            1219..1377
                     /locus_tag="ACG9CY_002566"
     CDS             1219..1377
                     /locus_tag="ACG9CY_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939022.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            1378..1740
                     /locus_tag="ACG9CY_002567"
     CDS             1378..1740
                     /locus_tag="ACG9CY_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939023.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            1777..2625
                     /locus_tag="ACG9CY_002568"
     CDS             1777..2625
                     /locus_tag="ACG9CY_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFS4939024.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            2615..4090
                     /locus_tag="ACG9CY_002569"
     CDS             2615..4090
                     /locus_tag="ACG9CY_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFS4939025.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            4356..4763
                     /locus_tag="ACG9CY_002570"
     CDS             4356..4763
                     /locus_tag="ACG9CY_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFS4939026.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            4766..4981
                     /locus_tag="ACG9CY_002571"
     CDS             4766..4981
                     /locus_tag="ACG9CY_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939027.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            4985..5365
                     /locus_tag="ACG9CY_002572"
     CDS             4985..5365
                     /locus_tag="ACG9CY_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4939028.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
BASE COUNT         2045 a          852 c         1183 g         1491 t
ORIGIN      
        1 cagaaatatg ttcctaacgg tttaaagttg attatagttg atggagtcgt aagaatttca
       61 caagatgaat gtgatcgctt tatggaagaa taccaaaaat aaacgcaagc cgtgggggca
      121 aagggattaa taggaggaaa taataatgga atcgacacaa aataaagcaa tcgaaaaagt
      181 attatcaaca attcttacag aagacacagc ggcagaatta gcaaacttgg aaggcaaagc
      241 gctagaagaa acgtttgaat gtttgtacga acaaatggac tatcaaaaat tgctaccaca
      301 agaaccaacg gtaagcggag tattgcgtgg cttgaacgat ttagtacaag cagagtttaa
      361 agaacgactt tctattgaag agtatcaaga aattttgtat cagcaagttg agttgttagc
      421 gagcttgcta gggcttgaac tggaggacgt tgaatgaaaa ctgaaatttg gaacggacac
      481 attataagat ttgtcgatat taatgatgaa tggtgggcag tggcgaaaga tgttgcggaa
      541 gcgttaggtc ttaaacaggt tactagagcc attcactctt tgcctaaaga tggggttact
      601 actagtaagg tcatcgattc attgggcaga acacaagatg taaacatcat caacgaaaaa
      661 aaatatttac cgcatggcat tcaaaagccg taaaaaagaa gccgaggcat ttcaagactg
      721 gattttcgac atcatcaaag aactacgcca gtccacagga cttgaaggct ttcaagtatt
      781 ccgcatgcta gacaaagaac accaaaaaga agcaatgact aaattaagcc acgctattac
      841 tgaaccaaaa ccagtagact acattaaagc gaatgtgata gccaataaag ctgtatcaac
      901 tatttatggt cattccaaaa tggtaaaaaa gaaagatatg actcctgaaa tgttggttga
      961 tcgtgaacct attttagatg agacagtaga attaatgact gttaaagaaa aatatgggtt
     1021 gcagttttca gtgagtgaga aaatttataa tcgttctgct gaattgcaga caacttagga
     1081 gcattaacat gatcgagaac aaagaaaaag ccttatccat aacagtcttg gcggacagga
     1141 taaaggcaac ttaattggga taaaacccga tattctttgt tccgattata ccatattaaa
     1201 aaatcggagg gaaccattat gaaacgaaac ttaattatag gaagcatttt aatcggcgga
     1261 attttcggct tattaccatt aattgcaaaa atagctatct ttgcaggatt agcgttatat
     1321 ctcttcactg cttatgacga gtacgactat caattaagaa ctggagggca taagtagatg
     1381 tacaaaacaa aactattaaa ccaactcgat agccttgaat tagaggaaat taatcaaggg
     1441 atcgctgaac tagaaaataa tatcgggaaa acctactttg gaaattcatt caatgaaaaa
     1501 ctaacagttt tgtatgtact caaaaaacat gcagaacaca aaataatttg tcgagaaatc
     1561 aacgagctaa aaaatcagat tttaaccgca tggttaaaca ttactgatat gcaagaagca
     1621 agggtgaaaa catttaatac atgggtaaag tatcagaatc aattaaaagg tgctgaattt
     1681 gttcgggatg ggttgaaata cgaacttgag cagttgaagc tcatggaggt gtctgaatga
     1741 tttataccag tattttgaaa aaggaggaga ggtttattgg caaataatta tcaagcagca
     1801 acggaattac aaaagtcggg atggtcaatt tatcccttat ctcctggaac taaaattcca
     1861 ataaaaggct ctagtggttg gaatgatgca actaacgatt ctaatcaaat taatgaatgg
     1921 tggacggaag atgcaactag aaatattggg ttaatgcttg aagatcatca aatggtggta
     1981 gttgacctag atcaacattc tgaaacacga aacggcgttg agaattataa aaaacttgct
     2041 tcaatgtatg atccatttcc ttctacttat acggaaatca caccaggtaa aggaatccat
     2101 ttcttcttta aaattcctga tggtgtaatt atccaacaag aaacaggtgc atttgctgat
     2161 atcttcgggc gtgatgataa aggtaaaagt ttaagcggga ttgatattat cactaaagga
     2221 gttccaatag cgccaactat gattatttca agaagtgttg ggagagcata tgaacctatt
     2281 gaaaacgtat ctctaaataa tattgcacca gctcccgatt ggctagtgaa cctactgcta
     2341 aaaaaaccgc aagaaataaa gactcaatac cgacccgtag caaaaacagg gacagcaaaa
     2401 aaattagata tgattgttca aggcgctggc gaaggtagta gaaacgatca tttaactaag
     2461 ttatgcggtt ggttactatg gcacggtgta gataatcaaa ctttagcaga attgatttat
     2521 acagcaaatg cgtacaatga accaccgcta aaagataaag aagtgaatca aattattcga
     2581 tcgatgatta aaaaagattt gagggggaaa gctcatggct cataagcata aattagatag
     2641 tattttagac tttcccgaag ccagcgagcg tgaagacaat atcattgaat tgaaaacctg
     2701 gatgtcacga ttaagatgca ataaagacga tcagattaaa agtaacagtg ttgtgaatgc
     2761 agaattgatt ttaaccaatg atagcaattt agctggaaca attgcttata acgaatttag
     2821 cggatacatt cacctattaa aagattctcc ttggatcaat cgtaccgctg gagaatggga
     2881 agacagtttt gaggatgcct taacggcata tattgaagaa aactacaatg tagtttttga
     2941 tgacaacaaa atacacaaag cagtagtaaa tgtggctaga aagaatgttt tcaacccagt
     3001 aaaagaacgt atcgaaaaag ttaaatggga tcaacagcct agactggaaa cactgtttat
     3061 tgacctttta ggggtagacg acaaccttta cacacgagaa gtaacaaaac gttggatagt
     3121 cggatcagtc gcacgtatct ataaaccagg aataaagttt gagatcgttc cagttttgga
     3181 tggaccgcaa gggattggta agagtaccgt tccagcatta ctttacacgg atgatttttt
     3241 caccgattca ttagattcgc taggtgaaaa gaaagatgat tatatgcagc ttcaaggcaa
     3301 tgtaattatt gaacttggag aattatcttc aatgaacaaa actaaaattg aacagataaa
     3361 aaacttcatc agtgccaaga tcgataaaat tagacctcct tatggtcgga atgtcatagc
     3421 ttgggcaagg caatgtgtat ttattggaac gtcgaatgat gggcagtatt tgaaagatga
     3481 tacaggaaac cgccgttttt atcctttgcc ttgtaagaat aagccaaaga tgaatccgtt
     3541 taaaacaaag gatgaatatt ttcttcaagt gttagctgaa gcaaaggtgc tttttgataa
     3601 aggacaacga atctatttta gtcccgatga agataaagaa gtcttagaaa ttgcaaaaga
     3661 ctatcaagag gatgccaaga tggagaatcc aattaaagaa gcaatcacta aatatttgga
     3721 aatggaaatc ccgtataaat gggaagaagc tccagcgtgg gcgagacgtt cttattatca
     3781 gaactatcct gacagtaaat gtaatgagaa aaatttacag catttctata atggagctac
     3841 tatgcaacaa gagttttatt tagttgatag cgtactcacc gctgatattt tggaagctgt
     3901 ttttgataaa caagcaaagg atttattgaa tggtcgatca gatgcagaaa ctaaaaagat
     3961 tgccttgatt attagcagta ttcctggttg ggaacggaag caactgccaa aaagaaataa
     4021 acgacgagga ttttacaata agtcaaatgc ctatgaaaat aaaaatcgag ctggaaagta
     4081 caaaaagtaa cgggtagtaa cgggtcagta acacctagcc cgttactagt ttacccttac
     4141 tgccccaagg gatacagcaa tttggtaacg gggtaacacc ttatttcctt aaaacttttg
     4201 aaatagttag ctaatatgct ggggaataat gttactggat tttacccgtt acctgttacc
     4261 aaaacgctag aacccttgtc cccgttggga ttgagtggta acgggtacct gttacgtagg
     4321 tgttaccacg tgttacgaat caaggaggaa agtaaatgct caatattatt tcaacaaaca
     4381 aagcaccgaa ctttcaatat acagatgaaa tggatcggtt tctaatgaac acgctggcat
     4441 ttagtgtggg attggtaaca gaagattatt ccacatttga tccagaagta ttaaagataa
     4501 tggaagaaga acctgattgg ctgcaggagt cagtagcgtg gtgtcaatcg ctagtagtgg
     4561 gatcattggt agatagtggc aactatgatg atacaggtga gctaatggat gaatttaatt
     4621 gtctgcttaa cctatatgat cgagcgagac aacgagagct tacatcgaat gaggataatt
     4681 tgtttttaaa catccatgat aagttcttgg ctttgctact aacagatgat gaattgataa
     4741 ctaatttatt ggaggtagaa taattatgca tatggaaagt attagatact tcgatggtga
     4801 gaaacacatt gagtattcaa acactcaaga agatgtagac tttattagtt ttgaggaaga
     4861 taaaatcact aaagttaatt ttaaagacgg aacgtgcttg aagatagttt cgccttatat
     4921 tgaatacaaa tcaaaatggg aacaagatga ttctgacgaa cgagatcgct ttggttggta
     4981 atctatggca gttaaaaaac aatgtaatca tgctggatgt aaaacattga ttgattatag
     5041 gcagaagtat tgtgggaagc ataaagcaaa gcaaacggca attaagcgtg aagaaagaaa
     5101 gcaaagcgaa ggtaaatatt ttcaattcta tcaaagtaga acatggcgaa aggcttcata
     5161 cttgtatcgg ttaaatcatc cagtatgtga ggattgccta gaagaaggct tgataaggaa
     5221 agctgatgtt gtagatcaca aaattgaact aaaagatgat tggaatagac ggttagatga
     5281 aactaatttt cgttctttat gccatgcaca ccataattcc aagacagcca atgaaagaca
     5341 aagacgagaa aagagtaccc tttgagttaa gggggcatag tgtgaaactt actgacaatc
     5401 gatgcctact cattttggta caaataaccg ttgtgaaaag gcataagggt aattacaaat
     5461 aacgattaca cttgtaatca caagtgaaaa agtatataat gaaagtagga gatttattga
     5521 aaaatacaat tgttattact gataaaacag ggaaacagcg caccattgag t
//
LOCUS       DAZULG010000143         5442 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000143 DAZULG010000000
VERSION     DAZULG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.147"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..569)
                     /locus_tag="ACG9CY_002573"
     CDS             complement(<1..569)
                     /locus_tag="ACG9CY_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS4939029.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
     gene            complement(903..990)
                     /locus_tag="ACG9CY_002574"
     tRNA            complement(903..990)
                     /locus_tag="ACG9CY_002574"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(956..958),aa:Ser,seq:cga)
     gene            complement(1135..1701)
                     /locus_tag="ACG9CY_002575"
     CDS             complement(1135..1701)
                     /locus_tag="ACG9CY_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4939030.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            1956..3287
                     /locus_tag="ACG9CY_002576"
     CDS             1956..3287
                     /locus_tag="ACG9CY_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFS4939031.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3253..3603
                     /locus_tag="ACG9CY_002577"
     CDS             3253..3603
                     /locus_tag="ACG9CY_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939032.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(3695..4114)
                     /locus_tag="ACG9CY_002578"
     CDS             complement(3695..4114)
                     /locus_tag="ACG9CY_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS4939033.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(4115..4459)
                     /locus_tag="ACG9CY_002579"
     CDS             complement(4115..4459)
                     /locus_tag="ACG9CY_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS4939034.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            4809..5405
                     /locus_tag="ACG9CY_002580"
     CDS             4809..5405
                     /locus_tag="ACG9CY_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4939035.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1513 a         1008 c          930 g         1991 t
ORIGIN      
        1 ccatttgata gcgagcgata attgttcaga ttaaataatt gataatcaaa tggcggatta
       61 tttgtcaaca caccgacagg attatcatat aaatgaagtc catctttcat actttcaatt
      121 actatcgatt tttctttatc agctaataac cagtgtaaag gtgacaaggg tagtttttca
      181 ctaaaatcga tattgactag attgatagta gccaataatt ctttagcttc gtctatggtt
      241 gagcattgcc ctagtatcca tggaataaac tcaaatggtg caacgttatc ttttccttct
      301 tgtaattctt tataatcagc attgccagga aaattcagtc ctgccatact caaccctttt
      361 tcatttgtag cctcataata gagtggatag ttggataccc cagctgctat tcctatcatt
      421 gcataatgtt tatctaaatt ttttaccttt ctaaaatcga aacgataatt tcttggagta
      481 atagtgacca cttcattata agacatctca taatcaaaat tccttccaaa ataatggtct
      541 ttcgttgcat aagtgattgc ggtacacatc gtttcccctc ctaaaacaaa tcgtttttac
      601 acttttatct tacatctagt atttttttta tcaaaaaaag gccaaagaag attgatttac
      661 tagcatttat aaaaaatagt actcaggggg aatcaaaaaa tattgtaggt taaaagtaca
      721 acaataaggg gctgtgacaa aagtcttgtc acagcccctt attccgaaca aacggtgttc
      781 aaaagcttgc atctgcggta ataagcccaa tcaaacgaaa aaataggaaa agctattttt
      841 gtttggccgt tcttattact tgatgcagga cagcttcttc acaacctcta atcgttgata
      901 taaggaaggt acaggatttg aacctgcgcg ccggtattag ccggttcgcc ggatttcgag
      961 tccggtgcat taccactctg ccaaccttcc gtaagtacta tttttaaact tttttcacga
     1021 aatgtcaagc ctatttctaa tagataaata acagaaatcg ctttttttag caaaaaagtc
     1081 cattgacagt ttgcagccaa cggacttacc gatcaaattc atactgatag aacactatct
     1141 gattatttca ggattatcta atgctaaaat cgagtcaatt gtccgtttga tcagtgcaat
     1201 gatttgttct tctgttggat gcatgatgtt tgatgacatc aatgacgcta agccagtcac
     1261 tgctacccat gtaccagtat gcagagcatc gacatattca tccgataatt gattgtatct
     1321 ttcatcttct ttaactattt cacggaaata attgtatgaa aaatcatgca ttttctttcc
     1381 gtccccatgt tcatcaatgt acaatgccat gtaaagacta tgatgctttt ttgcaaagta
     1441 gatatagttc aaaccgagat caatgatttt atcaccagta cgttcaatag ggaaaacatc
     1501 ttcttttagt tgttcaaaga tagaatccag caacgtattt tttaaatctt ccatgttttt
     1561 gaattccaga taaatcggct gtgtcgaaat ccccatcttt ttagcaatat ttctcgcagt
     1621 aaatccttta aacccttcct ttgcaacaac atcataagca gcttttagaa tttgatcctt
     1681 ggtatagact tttcttctca ttaaaactcc cccacttctt tactgcagta tatgttgttt
     1741 tattgtaccc tatttttagc attttttgca aacattttac tcaaaacgtt atattttaaa
     1801 tttttttcag tcttctttaa cttttttttg caaaattaaa aaaatttggt taaattcaat
     1861 gggttccctt acatttttag ttttgaccta aatgatagtt gtccaaatct ttcatttcaa
     1921 gttatagtta aagaaaatga aaaagaaggt gtgtaatgga aaacgaaact gacgttattt
     1981 atattcatcc tcaaaaacga atcgtttcac agaaaagaaa gtatttttat cttggtttta
     2041 caggtgtttt ttttcttttt attggcttgc tttctaatac tcctactgat aattggagtg
     2101 gattgctaac tattcttacc tcgccaagca atcttttgac agattatttt gctttaggtg
     2161 ttttcggaag cgcattcata aatgtaggga ttttgacctt actttctgtg cttttagctt
     2221 atcggcacaa ggtgatttta aacggtccct tatttgcttc gattctaaca gttaccggtt
     2281 tctctttttt tggcaaaaac ctttataatt ctatctcaat cattttaggt gtgtatctat
     2341 acgctgtatt tgtcaataaa cctttttctc agtacatcat gatcggactg tttggtagcg
     2401 tattatcacc ggtcgttagt tatatcactt ttggtatgcg ttttccttta ctgataggta
     2461 ttttattggg gaatttagct ggcatagcaa ttggtatact tcttccgccc ttagctgctc
     2521 aaactttagt tttccatcgt ggattcacac tttataatat tggattcacc tctgggttga
     2581 ttgcgatgac ttttactgct gttttgaggt tattcagcta ttcaatcgtc gaaaatacat
     2641 tggttttcaa tgaatatcac tttcctctca tttggattat ttttggcttt ttttcgctga
     2701 cagtcggaat tggtttctat tataattcct ttcggctttc ggggattcga gaaattttcg
     2761 attcatctgg aaaattgaca actgatttca ttgctaattc tggtattggt gctactttga
     2821 tcaacatggg cttagttggt ctgatgctgt cttcttatgt tttgctggtt ggcggtcagt
     2881 taaatggacc tgtcatcggt gctattttat ctgcggtggg tttcagtgct tttggttgtc
     2941 atttaaaaaa cagttttccg atactagtcg gaatatttat tgcttctttg tttggaactt
     3001 ttcatgagat caccagtact ggcatgcttg ttgcagctgt gtttgggaca ggtcttgcgc
     3061 ccataagcgg tttttacggc agtttttacg gtgtaatagc tggtgtgcta catattgctc
     3121 ttgtccataa cgtcagcact ttgcacggag gactaaatct atataacagt ggtttttcaa
     3181 ctggtttcgt tgcaggtatc cttgtaccga tactagataa ctttaccgct gtcagaaagg
     3241 aaaaaaagac acttggaaaa agaattatca aaaagaatca tcgatgagct tcttacttat
     3301 ttatattccc atgaaataac tgaaatacgt attggagtag aatttgctga ggaagggctt
     3361 ttcatagaga tacagggtac ggcagaaact cagccagaag atttagatgt acttgctgat
     3421 ttgctgaaca atcctcggga ccctagtatg gaaatctact atcaagaact attaggtaat
     3481 ccccgacatg atcgggaaga ttatcatctg ttaggtagtt tgattgatga cgtggaaatc
     3541 atgtatgccc agccgattat caatattaag atctttcgac gtgcttctca ttcctattat
     3601 taaccctatt ttctatttat aaaagactga atcaaaaaag cagctcctga aagttcgatt
     3661 aaaatctact ttcaggagct gttcaagctt gacattattg attttccaat ccttcataca
     3721 cttcatctgt tacaggttct aaaaactctg aattaccagc agtgatcgcc aaatgttcaa
     3781 accaactgtc ttttgtcgca ccatgccaat gtttaatccc tttgtgcgtt acgacgacat
     3841 ctccagccat taatcgtctt gctggctttc cttcttcctg ataccagcct tctcctccgg
     3901 tcacaagtaa gatttgatag ccgtctgtat ggacatgcca gtgattgcga caacctggtt
     3961 caaaagttac attacctaca ccaacagaaa cttcgggatc attcactaac atattcagat
     4021 agctttgccc ggtaaatact tgagcgtaag cttcgttttt ttcacctgct ggaaaaatca
     4081 cgccattgtg tatttcttcg tgttttgtca tcatttacgc ctctttctta tcttcgctat
     4141 tttcttctaa atagatttct tttgctaaac taaaagctga ccaagctttt ggccattcta
     4201 cataaaaagc taaatgggta atgatttctg ctatttcttc actagtaatg ccattttgct
     4261 tgccaatctt cagatgagct ggcaattgtt caaaatttcc tcctgaaatt aatgcagata
     4321 tggtgatgat actacgctga tgagcagata attcttctct ggaccatact tgtccaaata
     4381 aaacatcatc atttaattct gcaaattttg gtgcaaaatc tcctaattga tctcgtcctg
     4441 cggtttgttt ttctgccatt tctcttattc tcccttcacg actgttaggt gattcaacta
     4501 tggttctaag gcaactttat tttctattgt ttcttcgcat ttcattttaa atgtagtctt
     4561 tcagcctgtc caatgcttag tttattcagt cgatatgctt tttaagcata gttgctaacg
     4621 ctttttttat tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga
     4681 gatcaatctt ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa
     4741 aatggcgcag ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt
     4801 gatgaataat gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat
     4861 taggcctatc aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga
     4921 atgcgttaag aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca
     4981 tccaaatcct gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg
     5041 tcctgatatt tggcccttat gctggattta tctataacta tattggaatc tgcattggtt
     5101 cgattatcat ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta
     5161 gcgataagac atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc
     5221 tgtttgctct agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg
     5281 gattgacaaa catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt
     5341 cgattttctt atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg
     5401 gataatattt attttgtaaa attagtcatt gattgacaat gt
//
LOCUS       DAZULG010000144         5339 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000144 DAZULG010000000
VERSION     DAZULG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.148"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..578)
                     /locus_tag="ACG9CY_002581"
     CDS             complement(39..578)
                     /locus_tag="ACG9CY_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939036.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            complement(718..1398)
                     /locus_tag="ACG9CY_002582"
                     /pseudo
     CDS             complement(718..1398)
                     /locus_tag="ACG9CY_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(1720..1941)
                     /locus_tag="ACG9CY_002583"
     CDS             complement(1720..1941)
                     /locus_tag="ACG9CY_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939037.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(2039..2284)
                     /locus_tag="ACG9CY_002584"
     CDS             complement(2039..2284)
                     /locus_tag="ACG9CY_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939038.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            2524..3138
                     /locus_tag="ACG9CY_002585"
                     /pseudo
     CDS             2524..3138
                     /locus_tag="ACG9CY_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(3508..4509)
                     /locus_tag="ACG9CY_002586"
     CDS             complement(3508..4509)
                     /locus_tag="ACG9CY_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFS4939039.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(4668..4934)
                     /locus_tag="ACG9CY_002587"
     CDS             complement(4668..4934)
                     /locus_tag="ACG9CY_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4939040.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(4924..5280)
                     /locus_tag="ACG9CY_002588"
     CDS             complement(4924..5280)
                     /locus_tag="ACG9CY_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4939041.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1659 a          988 c          739 g         1953 t
ORIGIN      
        1 tgctatgaaa atagaatcaa catatttata atatctatct aatctatttt catttcaccg
       61 cagcatctta ttaaataacg tgtaaataag aatataaaag ttattgaact aattaataaa
      121 gatagaatca tcgtagttgg atacgtagat gaaacctcaa ctatagttct tttccccgta
      181 cctttaatag acactaaacc attgtcattt tctatagtgc tctttttccc atttactttc
      241 acaactgttc cccaataatt gaatacaggt aatgtgatat tttgctcatc ggcatcttct
      301 actgtataga ctattttgga attatatgct ttatgagatg cattctccca ccgatcacca
      361 acttttactt catgtttctt taaatgatct ttatatttca atgcatctaa tggtgcatag
      421 tcatataggc ctccagttct tgaggatgta taacgtgtaa catctttttt ggtcataact
      481 aaacgacctt tatacgactg aattttatgc gaataatttg cttctgttgc tgcagtcaca
      541 gtaaaaataa taagaactat tcctacaagg ctccacatac tttttttgtt gcttgttttc
      601 cactttaaaa acactatggt taagattaat gaaccaaaaa gcacttgaaa acccaaggtt
      661 ctcggttctg ttgcgaagtt tgaaaatcaa acgcgccctc tctgaactcc aaatatctta
      721 ggctgttatt cccattaata ccttgatttc agtagacacc gaaaagccga agagcgttcc
      781 atttcttcgg ttctttttat atattcctcg aatggtctcc atgcccttaa tcgtggaaga
      841 ggctgtacgg agactttgat aaaatttatt tcgtcgttta ataggtcgat ggtcttgttc
      901 tattaaattg ttaagatact tcacagttcg gtgctctgtc ttagtatata aacccacact
      961 ctgtaacttt ctaaaggcgg agccaagaga aggtgcttta tcggtcacaa ttgctttcgg
     1021 ctcaccaaac tgtttatgga gtcgttttaa gaaagcatag gctgcttgcg tatcccgttt
     1081 ctttcgtaac cagatatcta aggttaagcc gtccgcatca attgcacgat aaagataatg
     1141 ccaacgtccc ttaattttga tataggtttc gtccattttc catgaataga aggattgtct
     1201 atttttcttc ttccaaagat aatagaggac tttgctgtac tcttataccc aacgataaat
     1261 cgtagtatga caaacattta ttccacgatc atataacaat tcctgaactt cacgatagct
     1321 tagattgtaa cgcaggtagt aaccaacagc gacaataatg acgtcttttt tgaattgttt
     1381 gcctttaaaa tgattcatta ctctgtcctc tctgtctttt ttctcaattt tacactaaaa
     1441 tagatttttt ggaaaacttt gcaacagaac cgtttttact atatacttct tcccagaact
     1501 tgtccgtctc cttcaaatta tctctacctc tcgagttcat tgggtggttt gcccatttat
     1561 taaacatgga atcacctttc tcatactcat atatttatgt cctatttcaa ttatatttat
     1621 atatgcttaa ataaatttgt atcctgaaat ttattgtaat atgaagctgt attttacata
     1681 tagtaaccaa agagttaata aaataaaaag agcgaaccat tatagatcag ctcttttttc
     1741 attgctgttt ttaatcttat ctagaatcac ttcaaatgtt tcaaaggtat ttacatctaa
     1801 ctgacataac caaagcaagt acttcttttc tttgtaagtc caatcaatat tacggtaaga
     1861 ggacattaga ttatggtacc ttgctctttg ttcaactgaa gccaattccc agcgtttttc
     1921 aaatattttt tcttcttcca ttattgatcc ctcgcaagac gctacctcaa acttaaataa
     1981 tgggcagcga aagtctaatt ccttagatct aagtatagca aacaaatatg aaaaaaactt
     2041 agaaggtagt aaaagttctt ccgtcttttg ctttgcttct attcttaatg tgtttcgtta
     2101 tcggtgccct acaaagattt tcattaatta cacactcatt tattgttgtg tattttctga
     2161 catttctctc ttgatcaatt attttaattc catgggcatc ctgatagttc ataggagagg
     2221 agcggctatc agaaatagtt gcacctatct cttgaacttc ttttacaact tctggaaaat
     2281 ccatccatcc tgcttcagct gtcaaaagta ataatctata atatttatct ttccttttca
     2341 atctaagaaa tcgtcacccc attctttttc ttctattata aactatttaa cttttagaat
     2401 tcaaaaaccg tttgtctatt tagagtttac cctaaataga caaaaatata acattgaaaa
     2461 atttagagtt ctaacgtatg attagggtgt attataaacg ttatagaaaa gtagggaata
     2521 aatttgaaaa ttgcttatat tagagtttca tccatcgatc aaaatgaaca acgtcaaatt
     2581 gaagaaatga aaaagtttgg cgctgaaaaa atttttatcg aaaaacaatc tggagcgacg
     2641 attagtcatc ggccgatttt ccaggaagct ttagattttg tcagagagca agatattttt
     2701 attgtagaag ccattgatcg acttggacgc aattatgacg agattatcga ttcagtgaat
     2761 tatctaaaga agaagaatgt gcgacttatt atcaccagtt taccgatcat ggctgaagca
     2821 attggaaatc ctctcctaga taaattcatc aaagacttaa tcatttaaat ctcagccatg
     2881 attgctgaac aagaacgtac tgaatcgaaa cgacgccagg cacaaggaat taaaatcgct
     2941 aaagcaaacg gcgtctataa aggtcgtcct aagttataca gtgcagacac caaagatcct
     3001 caacgtcgcc ttgtctatag gagtatcgtt caggatcttg agaatggggt tgctatctca
     3061 aagattgcaa cagactacaa tgtgactagg caaacaattt accgaataaa aaaagaaatc
     3121 gaccaactaa ttgtatgatt tcttttttga atcacgtaat tattaattag ctttgtcatc
     3181 aatacagaca atcattttag attcatctag ctaagttgat tacatttatg ttaaaaagaa
     3241 tttagttaac ataaatataa ttgatgcata aatcgtcctt atttaaaggc atttaacatt
     3301 gacaacttag tataatgtat cttatgtaaa ctaataagat ttgaatttta ttttatacac
     3361 actcgataat agatcaattt tatggtcccc cgcatttacg attgcacaag agaaacataa
     3421 agtcgaccta ttagcatttc ttttcaattc aaacattaaa aaagtttata cctctgctac
     3481 taaaaaatga aattatctat ttaaaattta aaacggtaaa tctgattcat ccaaattgga
     3541 aaatggatca gcctgtattg gagctttgta tttctccata ataacagata tctttttttc
     3601 attttttaaa aaataccttt taaatttctt atagtcctct gaaatcaaaa tatagcgtga
     3661 tggatactca tcaaaagaag aatcgtattc ttcctttaaa attcccgatt tacttaattt
     3721 ttctagtaca tcaaatactc tatcactcaa gtaactcctt aaattgttat cactttccca
     3781 ttgaactact tttctataag ttgaattatc agttgaaaag atagaatttt catccataaa
     3841 cgcatatatt agcagcaatc tttcatcatt gtcaaattct attggatcaa aaatatctaa
     3901 gttgtcatca aaatctttat cagttccggt aacttttttg ttaattactg aaggtgtctt
     3961 gtaagaaata gaatctaaat caaagcgttt tgattctaaa ttattttgtg ctttctcaat
     4021 taaataattc actttttttg tgagaaacct tctgatattg tctttgctat atgttccata
     4081 aaaaccctta tcttctaaag agtccctgta cttcttaact tctatatact cttcagccaa
     4141 aacttgtgga actggttcat ctaagaaaaa aacccctacc tcgcctttgc tatttttata
     4201 aaagtaatca atttctgcta tagttcccga tctataatta tcaactggag aacccaactt
     4261 atggccaaaa atcgctaata atatatcact attttctaca atttgtttat taataacact
     4321 ttgcccagag ctattattta tataagtagg aatcgcattc gtttcccatc gaattggcat
     4381 caaaattact tttgtattat ctgtattttc tatattccat ctatatatgg tttcttctac
     4441 ttcattcctc tttgctgcta tatcacctgg tgatgctata aatactttta ttagatttat
     4501 ggttttcatt tattgtacct ttctctttat gtctctttaa agtaattttt gtataaaatt
     4561 tattgacttt tacactatag gacaactttt atctatttag tacaaaaagg cccactaaac
     4621 taattgatta gttaggagcc tattacaggt atattttcaa ctcgctttta agcaaattct
     4681 actaccagct ttttgcctaa tgccctagcg atatcatcaa gtttatcgaa agatacgtta
     4741 tcccctctct caattcgagc aatagtagat tgagttgtat gtgctttttg tgcaagctcc
     4801 gcttgagtta ggcctgcttc ctcccgtgca tgataaagag caaccgcagt ttcgagtttt
     4861 cttccttcac ggtcatatgc ttctgcaaaa tctttgtctt tttgcttttc ttcaatcaat
     4921 tttttaatca tcatttgtga accactcctc tctaagttct tttgcatgtt taagttcact
     4981 tattggtgtt ttctccgttt ttttagtaaa tccatgggta atgacatacc ttccattgac
     5041 cacatggaaa tagatagctc tttggatgtt tgatcctacc ttacttctta attcgtacaa
     5101 attagtgtct aattttttca cccatttcaa ccgttgagct actagcatcc cattcttttc
     5161 tgtttcttct attgtacgta aaagcttcgc actatctttt tttggtaatg tttccaacca
     5221 ttctaaaaat tcatcatgac cattaggtct tttaaatgtt tcaaaagata ttttttccat
     5281 acccaattat atcatatatt gattttatat tatagtatat aacataaaaa ttcgaatta
//
LOCUS       DAZULG010000145         5320 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000145 DAZULG010000000
VERSION     DAZULG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.149"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..1127
                     /locus_tag="ACG9CY_002589"
     CDS             120..1127
                     /locus_tag="ACG9CY_002589"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFS4939042.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1139..2143
                     /gene="neuB"
                     /locus_tag="ACG9CY_002590"
     CDS             1139..2143
                     /gene="neuB"
                     /locus_tag="ACG9CY_002590"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFS4939043.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2140..3288
                     /gene="neuC"
                     /locus_tag="ACG9CY_002591"
     CDS             2140..3288
                     /gene="neuC"
                     /locus_tag="ACG9CY_002591"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS4939044.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3261..3938
                     /locus_tag="ACG9CY_002592"
     CDS             3261..3938
                     /locus_tag="ACG9CY_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS4939045.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3889..5070
                     /locus_tag="ACG9CY_002593"
     CDS             3889..5070
                     /locus_tag="ACG9CY_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS4939046.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5083..>5320
                     /locus_tag="ACG9CY_002594"
     CDS             5083..>5320
                     /locus_tag="ACG9CY_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4939047.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIK"
BASE COUNT         1971 a          685 c         1041 g         1623 t
ORIGIN      
        1 tcaatcaaat gttgattcgt tacgttttga catcattatt gataattagt ttagttatat
       61 attttttaaa gattaagaat cgaattatag atgactataa ttcataggag gagatataag
      121 tgtttaaagg aaaaacaata ttagtaactg gcggaactgg ttccattgga agtgaaattg
      181 ttcgtcagct tttaaaatat gaaccaaaag ctatcagaat ttatagtaga ggtgaagaaa
      241 aacaatttta tatgcaacaa gagctctcaa atcacactaa tttgagattt ttaattggtg
      301 atatcagaga ctctcaaagg attgattatg catgtcgtga tgtggatatt atttttcatg
      361 ctgccgcaat gaaacacgtt ccagcaagtg aatataatcc tatggaagct gtaaaaacaa
      421 atgtgatggg aactcaaaat gtaatagatg cagcgatcaa aaataatgta aaacattttg
      481 taggtatcag tacagataaa gtagtgagtc ctactaacac catgggtgcg acaaagttat
      541 tgagtgaaaa attaataata gctgccaatc agtataaagg aaaaacagaa acaatatttt
      601 cttgtgtaag gtttggaaat gtcatggggt caagtggatc tgttattcct ctttttagta
      661 aacaaataaa aaacaataaa gatattactt tgacagatgt tgatatgaca cgatttatga
      721 tgtcaatacc ggatgctgca agattagttc tgtctgctgc tatgaattca aaatttggag
      781 aaacatatgt cttaaaaatg ccggcaatca atattttaac tttagctcaa tcattaataa
      841 aaatatataa tgatacacat gatgaaaaat atagaggtga aataaaagtt gtaggtatca
      901 ggccaggaga aaaattatat gaagagctaa tgactacaga cgaaattgaa cgctcatatg
      961 aaaatgataa aatgtatatc attccaccac actatcatgc tactgacgaa ctgtacaaag
     1021 attatgaaaa aattaaaatc actgcatact cttcagatgc aacagaatta atgagtggac
     1081 aggaatttat tagaatcgca aaagagaata atgtcgtgac ttggtaagga gttttatgat
     1141 gaaatcagaa gtatatgtaa ttgcagaaat tggtgtaaac cataatggaa gtctagattt
     1201 agctttagaa agtattgata aagctaaaga atgtggagct aacgcggtta aatttcaaac
     1261 atttaaaacg aataagctag tttccaaaaa ggctccaatg gcaaaatatc agaaggataa
     1321 tcttaaagag gacagttctc aatttgaaat gctaaaaaaa ttagaattat ctcaatctga
     1381 ttttattaga ataaaagatt attgtataca aaataaaatt gattttttgt ctactccttt
     1441 tgatgaagaa agtgcagact ttctaaaaca aattggtgta caaggattta aaattggctc
     1501 aggagattta acaaatttac cttttcttag aaaaatagat atgtatgggt tgccagtttt
     1561 attatctaca ggtatggctg atattcatga ggtcagtgag gctgtagatt gtttcataga
     1621 tagtccagtt actattttac attgtacttc aaattatccg gctagttctg aagatattaa
     1681 tttattggcg attaacacat taaaagaaat gttcgggata cccgttgggt actccgatca
     1741 ttcattggga tatgatgttg gtatttgtgc tgttgcgatg ggtgctaagg taattgagaa
     1801 acattttacc ttggataagg aactacctgg tccagatcat aaagcttctt tagatcctca
     1861 ggaatttgca gattttgtaa agcatattag aaacactgaa atatttttag gtgatggtgt
     1921 taagcgagca atgccttctg aacttagtac aaaagaagta tcaagaaaaa gcctagtggt
     1981 cactgagaat ttaaatgtgg gagatattct tacagagaaa tctcttacaa taaaacgtcc
     2041 tggaaatgga atagctccta agtttttata tcaatatatt ggcaaaaaag ttaaaagacc
     2101 aataagtgca gatagtgttc tgcttgagga agatgttgaa tgaaaaaaat acttatagta
     2161 agtggaacac gagctgatta tggaatatat aaacctgttg ctgagaattt actacaagat
     2221 aattgggatg tatcatttgt tgtttctgga atgcatttat cccataaata tggatttact
     2281 aaagaattga tcttagctga cggatttaga attagtggag aagttcaaag tttgttttta
     2341 gagaacaata agggaaacat ggctcggtct gtggcattag agataaatgg tttcacaaat
     2401 atatttgaac aaactagccc tgattttgta ttattactag gggatagagg agaaatgttg
     2461 gcagctgcga tagcttgttt gtatcttgga atcaaaacag cacatattca cggaggcgaa
     2521 gtgtctggta ctgtagatga atcaataaga catgccataa cgaaaatagc atctgttcat
     2581 tttgctgcta cggaaaaaag caaagagcgt ttaataaaaa tgggggaaga ttcttggaga
     2641 gtttttcccg tcggagctcc tagaatagat actattttaa ataatagatt acctagtttt
     2701 agagatataa aagaaaaata tcatctaaaa tttgaaaaga aaaaatacgt tttgcaagta
     2761 tttcatccag ttgtaaccga actagaaaat atagaaaaac aaattttagc tttgatcaat
     2821 tcaatggaac aacaatctca accaattatt tgcattttac ctaatagtga tgctggtagc
     2881 gaaattatca gaaaaatgta ccagaaatat ttaaaaacgg atattattta tattacgaat
     2941 ttagagccgg aggaatacct gactatatta aaagagtgca aatttatgat cggaaattct
     3001 tcttctggga taattgaagc tgcatcattc aaaataccag taataaattt aggaaccaga
     3061 caaaatggac gagaacaatc taaaaatatt ataaatgcaa atctaaatga aaatgaaatt
     3121 gaaactgctt tgaataaaat acaagatcct cagtttataa aaagcttgaa aaatgttaag
     3181 aacatctacg gtgacggcaa cagttctaga agaataacaa aaacattgaa tcaaatattg
     3241 aatgatgggg aagatattaa gtggattcaa aaacaaatag cttattaaaa tatactagtg
     3301 atccatacat tattattggg gctggcggac acgcaaaagt cattattgaa accttaagat
     3361 tagaaaataa gttaatatat ggattttttg atgataagca aaataaaatg caagatctga
     3421 aatatcttgg gaaaataaac gaattgaata gctgggcgga tagcaaaaaa aaaatctata
     3481 aatttattgt agctattggt gactgtgtag taagaaaaaa gattataaaa aaattgaata
     3541 tgccaagtga gcaatatgga atagctgttc atccaagtgc tatagtggca aaagaaattt
     3601 caattggtta tggaactgtt atttttgcga atgctgtaat taacccagat gctgtaattg
     3661 gagagcatgc tataataaac acaggtagca ttgttgagca tgattgtaga attaataact
     3721 atgttcattt atctccaggt gtatgcttag cgggtggagt acatgtaggt gtaggaactc
     3781 aaataggaat tggttctcaa tgtatccaga tgaagaaaat tggatcttgg tgtatgatag
     3841 gcgcagggag cactattgtt aaggatattc cctcacattc cttagcttat ggcaatccag
     3901 ctaaaattaa gaaagaagga attaattttg aattctagaa ttttattatc aagtcctcat
     3961 atgggaggaa cagaagaaaa atatgttgct aaggctttta aagataattg gatagcgcca
     4021 ttaggtgaaa atgtggataa atttgaagaa agtattacaa aatatactgg ttctaagggc
     4081 ggagcttctg cgttaagttc aggtacagct gccatacatc ttgccttaag actgcttaat
     4141 gttggaaaaa acgatattgt ctttgctcct tcatttactt ttatagctac agtaaatcct
     4201 attttatatc aaggggctgt accagtgttc attgactcag aacctaatac atggaatatg
     4261 agccctgttg cactagagag cgcattagaa gaagctgaaa gaaacaacca attacctaag
     4321 gcaatcatcg tagtgaattt atatggtcaa atggctgatt atgaacgaat tttagctatt
     4381 gcaaaccaat ttaatgttcc agtgatagaa gatgcagcgg aatcgttagg ttctacttat
     4441 aagggaattt cgtcaggctt atttgggaca attgggatct attcatttaa cggtaataag
     4501 atcatcacaa cctcaggtgg aggagctcta gtgtctagtg ataaaaaata tgttgagcat
     4561 tcaagatttt tagcaacaca agcaagagat caagcaccgt attatcaaca tagcgaaata
     4621 ggttataatt accgtctaag caatatttct gccggtatcg gacgtggaca aatggaagta
     4681 ttgaatgata gagtagctaa acgaagagaa attttcaatc gatataagga agctttttct
     4741 ggaattgata gaattgaaat gatgccagaa gtggatggca tggtgccaaa tcgttggttg
     4801 tctactttaa cgatcaatga ttataattct attgatcaag tagttaacgt aataaataaa
     4861 ctaaatgaaa aaaacattga aacaagagct ttgtggaaac cactacattt gcaaccagta
     4921 tttcaagggg taaaaatgta ttaccatgat aataatctta gtgtgtcaga aaagttattt
     4981 aatagcggct tatgcttacc ttctggaagt aatatgacag atagtgaaca agaatttgtt
     5041 attgatgagt ttaaaaaaat attcaagtag gtgtgatatt ttatgaacat aggaattatt
     5101 ggtttaggtt ccatggggaa aagaagactt agactcattc aagaacactt taatgatatt
     5161 ttactatgtg gtgttgatac taatgaggcg agacgaaagg aagttgaaaa ggaatttgga
     5221 ataccaactt ataatagtct aacagaggca aaaaatttgt ttgaaataaa ttcagttttt
     5281 gtttgtacat cccctataac ccatgaattt attataaaag
//
LOCUS       DAZULG010000146         5312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000146 DAZULG010000000
VERSION     DAZULG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.150"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG9CY_002595"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG9CY_002595"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS4939048.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG9CY_002596"
     CDS             complement(1297..2133)
                     /locus_tag="ACG9CY_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4939049.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG9CY_002597"
     CDS             complement(2317..3534)
                     /locus_tag="ACG9CY_002597"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4939050.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG9CY_002598"
     CDS             complement(3683..3844)
                     /locus_tag="ACG9CY_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939051.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG9CY_002599"
     tRNA            3917..4000
                     /locus_tag="ACG9CY_002599"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5312)
                     /locus_tag="ACG9CY_002600"
                     /pseudo
     CDS             complement(4240..5312)
                     /locus_tag="ACG9CY_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DAZULG010000147         5198 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000147 DAZULG010000000
VERSION     DAZULG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.151"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..695
                     /locus_tag="ACG9CY_002601"
     CDS             24..695
                     /locus_tag="ACG9CY_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4939052.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            754..1194
                     /locus_tag="ACG9CY_002602"
     CDS             754..1194
                     /locus_tag="ACG9CY_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939053.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1206..1874
                     /locus_tag="ACG9CY_002603"
     CDS             1206..1874
                     /locus_tag="ACG9CY_002603"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4939054.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1938..2561
                     /locus_tag="ACG9CY_002604"
     CDS             1938..2561
                     /locus_tag="ACG9CY_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS4939055.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2617..3003
                     /locus_tag="ACG9CY_002605"
     CDS             2617..3003
                     /locus_tag="ACG9CY_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS4939056.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3216..4607
                     /locus_tag="ACG9CY_002606"
     CDS             3216..4607
                     /locus_tag="ACG9CY_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS4939057.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1727 a          861 c         1095 g         1515 t
ORIGIN      
        1 atgatgaaaa aagggggaga aaaatgaatt ttttagttgt tttaaaagat actctgattg
       61 agcgatctac atttctatat gttcaattgc aggaaaataa aacattgctg catttttcac
      121 cagagttcca tctagagggg ctaacattag gagaaatcta tcaagcagaa gggctgtcgg
      181 ctgttttgca cttttttaag gtagaattgg aattaccagt aaaagattat atcgagcttt
      241 ctcttgaatc ttgggatcga gcagtggttg aactgtttcc agaaggttta atgatcgata
      301 caaacgatgg gaagatccat ctgactgcag cagaactgca aaaacaaatg cgttatcaaa
      361 tagatgacga aaattctttt tctattttta gacgtcagca aaagattcta aaaagctttt
      421 taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc
      481 caggtctgct tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta
      541 tacgtatgca acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag
      601 tagtcaacag agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg
      661 tccttcacca agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg
      721 tcataataaa tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta
      781 atacgatttc agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag
      841 ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc
      901 tatagaatcg gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta
      961 tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga
     1021 gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt
     1081 tatacttttt ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga
     1141 cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga
     1201 aaagaatgaa attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact
     1261 ggacaggatg gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg
     1321 gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac
     1381 gtgcgattct taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa
     1441 taaaaacatg gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata
     1501 agatgaaaga aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag
     1561 ttccgccgtt aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt
     1621 tgatcccata cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag
     1681 atcatctagc accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata
     1741 gcatccgagc actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg
     1801 atatcccaaa tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca
     1861 aaaaaatact ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg
     1921 aatggaacaa aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata
     1981 cgtatcccgc aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg
     2041 acaaaaaaga aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat
     2101 caaacttgaa tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg
     2161 aagtttcccg tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg
     2221 gtcgtcattt catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac
     2281 atcgattggc acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac
     2341 ctgatccagc atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga
     2401 tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt
     2461 gtctttacga catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc
     2521 tgaatgaaat ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga
     2581 tatgttataa ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa
     2641 gttgtagaag catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg
     2701 aaagtaggct tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc
     2761 gtaggagcac gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg
     2821 agtcagatca atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa
     2881 gaagtggacg agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg
     2941 acagagatcg ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa
     3001 tagacagtaa aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac
     3061 taattgtaag caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg
     3121 aaaaaaattt tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg
     3181 atggacttgt cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag
     3241 tggttctagt ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa
     3301 aattttcaac acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc
     3361 aattgatctt tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg
     3421 taggtcaagg atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga
     3481 tcattggtgc attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg
     3541 tattgacttc tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa
     3601 tgctgttcgg actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct
     3661 ctgaactggc aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat
     3721 cctttatccc taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg
     3781 gagtcgttat tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc
     3841 caaaagaagc agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga
     3901 tcgtgacgct tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt
     3961 tggcaacaag tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg
     4021 tagcattgct tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc
     4081 caatcaacta tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa
     4141 gtgctggagc tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg
     4201 caacagctaa tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg
     4261 tttatccagc tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt
     4321 tggaatttat tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag
     4381 tcggtggggg cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag
     4441 caatcgttgg gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa
     4501 acgtcagcgg tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc
     4561 gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt
     4621 gtgatagaag tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa
     4681 tttttgtgaa atttcggctt atttccgaga agctacttct gaaacaccgt gaataagagg
     4741 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4801 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gtttaagaaa
     4861 gaggtcgtga gcctgccgtt tcactctgag tcacaaagag caatttcaaa aaatagcttc
     4921 tcatgtttgt tgaatattgt gtcttgtgac cgaggagttg tcaggagaac accgtttacc
     4981 agaggttgtg acttgttttt agtcacgacc tcttttttta tttattccat ataaatacac
     5041 attatttatt ccgaaaagtg aaagcgcaaa taacaaaaac aaccaactta tttattatta
     5101 aagaaaagcg tatttaagta ggacgattct gttgagagct ttcgtagatt ttcccatttt
     5161 cctatggtaa actgacaaag ttgagaatgt tagaacag
//
LOCUS       DAZULG010000148         5135 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000148 DAZULG010000000
VERSION     DAZULG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.152"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(406..780)
                     /locus_tag="ACG9CY_002607"
     CDS             complement(406..780)
                     /locus_tag="ACG9CY_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4939058.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="ACG9CY_002608"
     CDS             complement(803..2203)
                     /locus_tag="ACG9CY_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFS4939059.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="ACG9CY_002609"
     CDS             complement(2207..2617)
                     /locus_tag="ACG9CY_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4939060.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="ACG9CY_002610"
     CDS             complement(2635..3126)
                     /locus_tag="ACG9CY_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4939061.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="ACG9CY_002611"
     CDS             complement(3136..3927)
                     /locus_tag="ACG9CY_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4939062.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="ACG9CY_002612"
     CDS             complement(3920..4693)
                     /locus_tag="ACG9CY_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4939063.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>5135)
                     /locus_tag="ACG9CY_002613"
     CDS             complement(4849..>5135)
                     /locus_tag="ACG9CY_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4939064.1"
                     /translation="NEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGIT
                     NEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1638 a         1038 c          674 g         1785 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt
     5101 ttcatacaaa tatagtactt tttctatctc attga
//
LOCUS       DAZULG010000149         5084 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000149 DAZULG010000000
VERSION     DAZULG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.153"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..868)
                     /gene="iolB"
                     /locus_tag="ACG9CY_002614"
     CDS             complement(44..868)
                     /gene="iolB"
                     /locus_tag="ACG9CY_002614"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFS4939065.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(907..2823)
                     /gene="iolD"
                     /locus_tag="ACG9CY_002615"
     CDS             complement(907..2823)
                     /gene="iolD"
                     /locus_tag="ACG9CY_002615"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFS4939066.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2834..3868)
                     /gene="iolC"
                     /locus_tag="ACG9CY_002616"
     CDS             complement(2834..3868)
                     /gene="iolC"
                     /locus_tag="ACG9CY_002616"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFS4939067.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4025..5041)
                     /locus_tag="ACG9CY_002617"
     CDS             complement(4025..5041)
                     /locus_tag="ACG9CY_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4939068.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
BASE COUNT         1336 a         1268 c          909 g         1571 t
ORIGIN      
        1 taactaaacc taactaaact ttatgtatct aacagcttaa taactacttg cgctgcaaga
       61 tccattcgtg tttcttttca ttgtggaatt tccaaattcg ctcaggtccc gccatcacat
      121 ttaaatagta ggaagtatac ccgtcaggta ccccaactgg atgatagccc ttaggaactt
      181 cgacgacatt ttgatgctct accgtcatcg tttcatctag tgaacgatct tccgtgtaga
      241 cccgctggaa aacaaatccc tgctctggat caagctcatg gtaataagtt tcctctaaaa
      301 aagactcatg tggcagattc tcttgatcgt gcttatgagg aggatagctg gaaaaattac
      361 cgccctcggt ataaacttcg acgaccagca ggctgctggc agtatcgaca ttatccggta
      421 aaatgttgtg aacaagtcgt ttattctgat agagtccacg ttcttcgacg gtattatcct
      481 ccgcgcgaat cacacttatc ggcaaagctt ttttcgctgg tgaatagcaa agcgccaatc
      541 ggacattgtc tgtagttgcc acaagttcaa acgaatgctc cgccggaaga taaatactgt
      601 cggttggaat tttttcaaaa acagattctc gtgttcctaa atcttcatac caagtgtctg
      661 ctgctttaac cttaattttt ccagtcaaaa tgacgataca gacctcctgt tgactgccgg
      721 tttccttgta agaagctccc ttttccagct ccaaaatctg aaattcgata taactcaaag
      781 cagaattaga ctgccgaata ttttggattt gttttactcc aggtgccagt tctttgttta
      841 atggttgatg cttcaatgtc ttttccaatc aatccactcc ttattttcaa tcacaaaaag
      901 caatatttaa tactttctga tagtggcaag tttcgcttgt ttggctttgt aagcctcctg
      961 catggctgct ttacccgata cctctgagat accgacgttc caccagctgc cgtcgtagcc
     1021 ttctgtcatg gttttaggaa gtacctttat ctcaatcaac gtcgatcgcg tttgcttctt
     1081 cgcatcctcg accgctgctt gtaattctgc aatcgagctt acacgatacg tttttgcacc
     1141 atagccttca gcaactttgg cgtaatcgat gttcatgatc tcgtccttgg tatttctgaa
     1201 ttcggttcct tggctagctc cgccattatc catctgtaaa ttattgatac agccgaaacc
     1261 agaattatcg aaaagtacaa tgttgatttt tttatcatat tgcaaactag aaaccagctc
     1321 gctatgcaac atcaagaaac tgccatcgcc tactaaggca tagacctctt gttctggtcg
     1381 agccattctt acgcccatcg caccagcgat ttcatagccc atacaagaat aaccgtattc
     1441 caaatgatag gtattctcct tcacagggtt ccataagcgc tgcatatccc ccggcaatga
     1501 accggctgaa ccgatcacga tcgcatcctc atcaacaaaa tcatttaaat gaatgaagac
     1561 ctctgtttgc gtcaaattcg tagccaacgc atcagcatat tcattcaaga tttcttgaga
     1621 aaaatgatct ttgatttctg gcgtaaaatc tttacgctta aaatgcgcgg cttttaaccg
     1681 atttcgctca ctgatccatt cttccttcag ttcatcgatc ttctcgccat aggcggtttg
     1741 gtaaccttcg atcattgggg caagctgctc caaggtcgct ttcgcatcac caaccacttg
     1801 aaaggcttcc agcttagcgg cctgttgtct gcttacgtta atattcatca ttttcgcttg
     1861 ttcaaaatta aaaatacttt tagaagctga ggtaaaatca gtgtagcgtg tgccgatccc
     1921 tatgatcaga tccgcttgct gcacagcttt attagcagcc gaagttccca aaatccccat
     1981 tccgccgaga ttgtttttaa aatcccacga aacagtcgat ttacctgcgt gcgtttcaac
     2041 tagcggaatc tgacaatctc ttgagatttg ctcaagaatc tcaccagcct ccgaatattt
     2101 gcagccgcct cctacgatga tgacaggtcg tttactggcc tttattcgtt gactcgcttc
     2161 agcaagctca cgagcggtcg gaatcctgcg atcaatataa tgtactcttt ttttgaaaaa
     2221 ttcctccgga taggcatagg cttctgcctc cacgtcctgc ggcaaacaaa tcgttgccgg
     2281 acccatcgtt gccgggttcg tcatgacttc aaaggcccgc aataacgcgg acatcaactg
     2341 ctctggtcga ttgatgcggt cccagtagcg agaaaccgct tgaaatgcat catttgtcga
     2401 aaaggctaca ctgcttgcat gttccagctg ctgcaagacc ggatcaggct gacgtgtagc
     2461 aaacgtatcc gccggtaaaa aaagtacggg gatattattt gcgaatgccg tacccgctgc
     2521 tgttaacaga ttggccgcgc ccggtccaac agatgttgat acggcaaaaa ttttcttgcg
     2581 taaattctgt ctggcaaagg cagtagccgc atgggccatt ccctgctcat tttttccgct
     2641 ataacttttc aaatgtccgg gctctaattc cagcgcctga ccgatcccca aaacatttcc
     2701 gtgaccatag acatgaaaga taccttcaac ataaggcgtt tctttgccgt cgatgctgat
     2761 gtattgttga ttcaaaaatt ttagtaatgc ttgtgctgtt gttaaacaaa ccgtcttttc
     2821 catttttttc accttaaatc cgagcgatca tttcgccgta tatttctttc tccaatgtaa
     2881 taaaatcttt tacctcctga gtcgtcggta aagaatctga acagttattg cttttgacca
     2941 tcattgatgc ttccgctgag ccaaactcta aacaatcgat gatctcccag ccttcgaata
     3001 aaccgtataa gaaacccgag ccgtaaccat ctccgccgcc aaagcctttg cgtgcttcca
     3061 ccggaaacgg cttgattgaa aactgctgct gatcggccgt ataagcagtc gatcctttca
     3121 ttccatgttt gattacgaca atctcagcac cacaactgtg ccaaaaagca gcgctttcat
     3181 tatctgacat gttaggctgg atcaacttct cagttagatc gaactcttct cttgaaccca
     3241 tgataatgtc tgattcctta gcaatcatcg aatagtaaat tgaaatatca tcgttgttct
     3301 tccagttata agcacggtaa tcaatatcaa aaataatttt gacattattc cttttcgcca
     3361 gcatcactgc cttgattacg gcttctctgg acgggctctc tgctagtgca gttcctgaga
     3421 ttaatagtgc ttttgaccgt ttcaagtagt cctcatcaat atcctcaacg ctaagctgca
     3481 ggtctgcggc ttgatttcga tacatcaaaa tattactttc ctttgatgag agcatttcgg
     3541 taaaagtcag ccccagcttt tcaccatgct gcgctttgct gatctgagac gtatcgatgc
     3601 cttgttcatt gaaataatcg atcacatatt cgccaaactg atcgtccgag actgttccga
     3661 taaaaccagc tttcatgcca tgtttagtaa cacccacagc aatattggca ggagatccgc
     3721 ccacaaattt ttcaaacata tggacctttt tcaatggttt aaactcttct ttgacttgat
     3781 cattgtaggc aggattaaaa tcaatagcga ctcttcctaa aagcagcaag tcaaattctt
     3841 ttgattggtc aaactttata taactcaata tatccacctc tttatccgtt cttctatttc
     3901 tgaggcaagt atagcacgcc aaaacaaagc gtgcaatatt tattttagaa cgcaatattg
     3961 cgtgctgttt gaaaatgacg gcaaaaaaaa cgtgaatcgc cttacatcac gattcacgcc
     4021 tagattaaat attgtatttt gtcttaataa ttatatcagt aaatagcgca tcttttgtaa
     4081 ccgttttatt attttgcagc tgattcgcta acatcagtaa tgaacgatag ccttgaacaa
     4141 atccatcttg atcgattaga aaatcaacct gatcgttttt cagcatctcg atattgttct
     4201 tagtcaaatc ataaatgatt acaaagggcc gcttgctttc atcgattttt tccaacgctc
     4261 gggcaattcc cgcttgtccg ccagaaaaga cgactacgcc atctaagttt gaataattct
     4321 ccaaggtgtt gcttaaaatc ttttcaacct cagcactcga atcaaagctg ctctgaacac
     4381 ccgtcagctc cagttcagga aacgactctt tcaattcttc aacaaagcct gccacccgta
     4441 agctgttgac gctatttcca aagtagccgg taatcgctag aaccttgccc tttccttttg
     4501 tcagcatacc taacaatccc gcagccgttt ttccgctttt gaagttgtcc atcccaacaa
     4561 aacactgtcg attcgtacca agaatatccg aattaaaggt aatgatgagg atgccttgat
     4621 cggtcaattg attaattttt tctctgatac catcactatc tactggcatg atcgcgatcc
     4681 cttttactcc aagctccacc aatcgatcca acgcctgcag ctgggccgct tcatcgaccc
     4741 cgtctacctc ttctaaaaaa caatcaatgt ttcgcttttt taaatccccg ataatagatt
     4801 ccagacctga tcggatcgga atcataaaag aagatttcgc caattgagtg acaatcccca
     4861 gcttgagcgt tgtctctttt atgaccttct ttttctttgg aacatagttc atttccttgg
     4921 cgattgctct gattctttct gcaacctcag gattgatcct tcctcgatcg ttcaatgccc
     4981 gatccactgt cccccttgac actcctgctt tctcagctat ttctttaatt gtacccgcca
     5041 tcttgcctct ccttttttcc tattgtgttt attttatcac aaaa
//
LOCUS       DAZULG010000150         4956 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000150 DAZULG010000000
VERSION     DAZULG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.154"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..828)
                     /locus_tag="ACG9CY_002618"
     CDS             complement(55..828)
                     /locus_tag="ACG9CY_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4939069.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            972..1322
                     /locus_tag="ACG9CY_002619"
     CDS             972..1322
                     /locus_tag="ACG9CY_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS4939070.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1303..2019
                     /locus_tag="ACG9CY_002620"
     CDS             1303..2019
                     /locus_tag="ACG9CY_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS4939071.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2019..3467
                     /locus_tag="ACG9CY_002621"
     CDS             2019..3467
                     /locus_tag="ACG9CY_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4939072.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3537..4046)
                     /locus_tag="ACG9CY_002622"
     CDS             complement(3537..4046)
                     /locus_tag="ACG9CY_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939073.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4036..4761)
                     /locus_tag="ACG9CY_002623"
     CDS             complement(4036..4761)
                     /locus_tag="ACG9CY_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939074.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1703 a          843 c          998 g         1412 t
ORIGIN      
        1 ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat
       61 cagatcaaaa tacttcaatg cgtggacaat tccgccttct gtatttttct ttgtgataaa
      121 ggtagaaagt tcttttaatc cgtcataggc gttgtccata gcgattttgt tgtcgcaagc
      181 ttccaacaag gcaaaatcat tcggaccatc gccaaatcca aaagtaggaa ctttttccag
      241 ctgaagttct ttttgcagac gtttgacacc tgcccctttt gagattcctt tcttcactgt
      301 atcgatcgag taaggaccat tccgataaaa agttagctca ggaaaatgtt cgtgatagta
      361 ttcgtcacct gattgagata agatcagcaa catatttact gtgttttctt taaagcctaa
      421 aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct
      481 atgaccggta caccagattt tctgatcgtt atagtaagaa agttcatgac cgttcaactg
      541 tacatgatca tacatttttt gacattcttc tgaagaaaat gtttgagaat agatttcttc
      601 gccgtctact cgaatgaaag aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc
      661 catgatagag tggatttctg cttctgtacg tcctgtagcg atgacgggga gaacattgtt
      721 ttcttttaat gcgttcatag cttttgccac ttcgggtgtg atttttgatt tcgcatctag
      781 taatgttcca tcaagatcaa agaaagtgat tgcacgataa tttgacacaa ataaaactcc
      841 ttttttattg gaataagttt atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa
      901 gaattcctga acatattggt tcagtcaaca atttagagta tactataaga agagaattta
      961 ggagggaaag aatgattttt ttcagtattg gtatcttatt cttaataatt ggcagtcttt
     1021 ttcggattct gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg cctcttgccg
     1081 caaaaacaga cgctcattgg cggctggctc aaaagacaag cggaaactgg ttttttctta
     1141 tgggcttact gatggcattg atcggctatt atttaaagac atctgggcat acgaattatt
     1201 ttctgattga aatgcttctg ctagtatttc caattatgcc tatttttatt atgacagaaa
     1261 agaaattaca gaaatatgac ctggaaactg gagggaacga taatgaatat tttaatgatt
     1321 gaagataatc aatctgtttc tgaaatgatg cagatgtttt tcttaaatga aggttgggaa
     1381 gcaactttca agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat
     1441 tgggatatga ttacactaga cctgaatttg cccacactgg acggtatgct agtaggaaga
     1501 gagatccgca aagtttcaag tactgtgccg attatcatgc tgacagctag agattctgaa
     1561 agcgatcagg tgatcggctt agagatgggg gcagatgatt atgtcacaaa acctttcagt
     1621 cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca
     1681 agagaagaag cagcagtttc agatgatcat tttgatatcg agactagtca tttcaagatg
     1741 aatacgaaaa cacgagaagc gtatctggac aaccggccaa ttgaaggact gacgcctaaa
     1801 gagttcgatc tattgtttac tttagcaaaa aaaccacgtc aagtattctc tcgggagcag
     1861 ttgcttgaat tagtctggga ttatcagtac tttggtgatg aacggacagt ggacgcccat
     1921 atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca aactgtttgg
     1981 ggagtcggct acaaattcga tgattctggt gttgcctaat gcgttatttg tatcagcaac
     2041 tcttagcttt ttgcggaatg gtcgcgttga tcttattgat cgttggagtt tcttttacac
     2101 aactgacaaa acaaacgatt gaagaaaaca attaccagca actgtttgga tatgctgaat
     2161 cagttgaaaa gacaacgcaa acctatgccg atacgctccc gcagtttagt caagatcagg
     2221 cgtttcaaaa tgcattgctg ctgacagagc aagtactgac agaacaaaat gtgaacttta
     2281 tatttatcga taaaaacgaa cgtgtcattt atccgactaa tacagcttta ctgaatttta
     2341 ccttaacagc tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga
     2401 ataaaaatat tttaggacaa gcacaggcga cctcttacgc acttgtgcca tttaatttga
     2461 atcatgaatt ctatggagca cttgtagtta gtcaacctgc gcgaaatatc gataacagcg
     2521 ttcgtccaat tacactgaac ctgttcaaag gatttatttt ttccagtatc gttgcagtta
     2581 ttgccagcta tgcttttgca gcattccaag ttaagcgtat caatcggtta aggaatgcga
     2641 cgaaagaagt gacgaatggt aattttgatg tacagctccc cgtacatgac aaagatgaat
     2701 ttgatgagtt agcagatgat ttcaataaaa tgacgaattc attgaaagaa tctcaagcag
     2761 aaattgaaga acaagaaaat cgtcgaagac aatttatggc agatgcttct cacgagatga
     2821 gaacacctct tacgacaatc aacggacttc tggaagggtt agaatacaat gcaatccctg
     2881 aaaatcagcg agaaaacgcg attaaattga tgaaaaatga aacagagcgt ctgatccgtt
     2941 tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta
     3001 aaaaattcaa tggaacagaa acattgaaaa atattgttgc acagttagaa gctaaagcag
     3061 aagcagcagg agatacattg acattgaaag ccgttgatga tattgatgtt tacgctgatt
     3121 atgaccgatt tgtccaaatc atggtcaata ttattcaaaa cgcgatacag tttactgaaa
     3181 acggccaaat cacgattact ttagaaaaag gctatcttga aacgatcatc acgattgaag
     3241 atacgggaat tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt tattataaag
     3301 tagatccttc aagaaaaaat acaaaatatg gtgaatcagg attaggttta ccaatcgtgc
     3361 agcaacttgt acgcttgcat aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa
     3421 caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta
     3481 taaaagatca tgaatcgatc ttctataccg cttgttttta ctataaaatg attgatttag
     3541 cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc atgagtaaag
     3601 agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag tagctcttgg
     3661 atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt tcgcttggaa
     3721 agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc agctgccgca
     3781 caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg atcttgtttg
     3841 atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata aggaaacatc
     3901 ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac ctcttttttc
     3961 aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt caagtgttca
     4021 gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca aatgatggat
     4081 aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc caataagagt
     4141 ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa
     4201 aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga tatcacggat
     4261 atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa gcgcttcata
     4321 aactgactgg aagctgacga tccacaataa aaccaagata caacaaagaa agagaggaaa
     4381 gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag tcaggcgcca
     4441 attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa
     4501 ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag ctaattctaa
     4561 agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat cttggacttg
     4621 attggctaaa gccgattgcg aatggtaaag agtcaggcat gctatcaata aaaaagcaga
     4681 aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta atcgagcagt
     4741 agcatagcgg attattttca tacttttcac ctctataaaa agtatagaaa atgaatatga
     4801 ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa
     4861 agcaagaggt tgtgaagaag ctgccctatt ctattaccgt agatgcaagc ttttgaacac
     4921 tgtttattcg aaaagaggag cagcgacaat aatttt
//
LOCUS       DAZULG010000151         4902 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000151 DAZULG010000000
VERSION     DAZULG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.155"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..744
                     /locus_tag="ACG9CY_002624"
     CDS             58..744
                     /locus_tag="ACG9CY_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS4939075.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            746..1690
                     /locus_tag="ACG9CY_002625"
     CDS             746..1690
                     /locus_tag="ACG9CY_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS4939076.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1889..2533
                     /gene="pcp"
                     /locus_tag="ACG9CY_002626"
     CDS             1889..2533
                     /gene="pcp"
                     /locus_tag="ACG9CY_002626"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS4939077.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2612..3889
                     /locus_tag="ACG9CY_002627"
     CDS             2612..3889
                     /locus_tag="ACG9CY_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939078.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            4147..>4902
                     /locus_tag="ACG9CY_002628"
     CDS             4147..>4902
                     /locus_tag="ACG9CY_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4939079.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         1583 a          887 c         1049 g         1383 t
ORIGIN      
        1 ttttatgtgt tttgctttat aattaaaaaa atattgtcat tttatggagg aaaatatatg
       61 gaaaattatc ttagcctttt aggcgtcttg attgttattc tcgggtttgc atttaaatta
      121 gactcgatat tgatcgtaat ggttgcttta gttgttacag ctttgactgg cggtcttggc
      181 attcaaggaa tgttagaact tttaggtaca agttttgtaa acaatcgcgg gatggcgatt
      241 tttatcatca ttatgttggc aacaggaacg cttgaaagaa atggtctgaa agaatcagcg
      301 gcttccttga tcaagcgatt caagaaagta tctgctggta tgattgtaga tatttatggg
      361 atctttcgaa tgatctttgc ggcttttaac gtgagttttg gtggagttgc tggatttgtt
      421 cgtccaatca ttttaccgat ggcattgggg accatcgaat caaaagggtt agaagtccat
      481 cctaagcatg aagaagaact aaaaggtatg gcttcagcta tggaaaatgt ctgctggttc
      541 tttggccaag tactattcat cggaggagca ggggcgttgc tagtccaatc aacactgaaa
      601 gatctaggat atgacgtaac attgggacag cttgctttag tggaagttcc agtggctatc
      661 acggctttag ttgtggcaag tgtctacttc tatcttaaag aaaagaaatt agcgaagaaa
      721 tattatccag gaaaggggca ataagatgga cttttttaca aatcctgata ttttattagg
      781 cgataaatta ctggagatcc tttatatcgt gatgggactc gtattgattt acactggagt
      841 aagaaattta acagataaaa ccaatcctca tcgttatggg tcagcctttt tctggacggt
      901 cttaggggta gttattgcag gaggaagatg gcttccggca atcgtgaacg gtggcttgat
      961 ctttgcgatg accataccag caattgctaa gaaagtcagc aaaggagaaa gccggcttcc
     1021 atcaaaagaa tatatggaaa aaatgtcaga caaactagga atgaaaattt ttattccagc
     1081 tttgagtatc ggtgtctttg cgatcttgtt tgctctattt accaatttag gtgctttagt
     1141 cggcgtgggc gtcggtgttt ttgcagccat gctaatcttg atgttctttt ctcgagataa
     1201 tcgaccaagt acatttttag atgatgcagc agatatgctg ggcacagtag ggccgttaag
     1261 catgttgcca atgcttttag cttctctagg tgcggtattt acaagcgctg gagttgggac
     1321 agtgatttcc aatgcagtag gtacgatcgt tccagaagga aatgtgaaca tcggcatcat
     1381 catttacgct cttggcatgg tgatatttac ggtaatcatg gggaatgcat ttgcggccat
     1441 caccgtgatg acggttggga tcggtgcacc attcgtattt agtcatggag cgaatccagc
     1501 gttaatagga atggttgcat tgacttgcgg tttttgtggg accctgctga caccgatggc
     1561 tgctaacttc aatatcgtgc ctgtagcgat gctggagatg aaagacaaat acggagtgat
     1621 caaaaaccag ttgtttattg cgttatttat gctagttttc caaattgctt atatgattct
     1681 atttaaataa tagaagagag gggttgtgac agaataattg tcgcaacctc ttctaacatt
     1741 gtagtggaac actatttttt tataagaata tattaagttt gatctgttat gttatttaaa
     1801 ctattgtttc ctttatttac gctgttcaag gaaaatggta tgataaaaga taaccatatg
     1861 ttaaatcaat cggacggagg aacaacagat gaaagtacta gtaacaggtt ttgatccatt
     1921 tggtggggac aaagtgaatc cagcctatga agcagtaaaa aaaatgccag atgaaatcag
     1981 cggtgctgaa atcatcaaag tggaagtacc gaccgtattc gaaaaaagca gtcaagtagt
     2041 caaagaagcc atccaacagc accaaccaga tgtcgtgatc tgtgtcggac aagcaggtgg
     2101 gcggtcagca gtctcgttcg aacgtgtcgc aatcaatcta gcagaagcaa gaatccctga
     2161 taatgaagga aatcagccat tcgatacggc tttagaagaa aacggtccag ccgcttactt
     2221 tacctcattg ccaatcaaag caatgacaaa aaatgtacaa gatcatggtc taccagcgta
     2281 catctcttat acagcaggaa catttgtctg caatgatatc atgtatcgtt tgcttcattt
     2341 gatcgaaaca caattcccgg caattcgagg tgggttcatc cacgtgccat tctcacccga
     2401 tcaagtgatc gatcgtccag tgggtacgcc ttctatgtct ttagaagaca ttgcttcttc
     2461 tttgacttat gcagttgaag cagcagtaga aaataaagaa gatatttccg gaaatgccgg
     2521 aacaactcac taatttagca agaaacactg gctggttgct aacgttatgt tagtagccag
     2581 ctttttgtaa ttagaaagga aagaaacagc cgtggaagaa tggcaatccg tctttgaaga
     2641 atggtttcca aaagaaatca gcaaaagcta tcccatcaaa atttctaaac aatacacaag
     2701 cagtcaacgt tgggagatct atgcaaaatt aacgaaaaaa cagcgggaac tagtcgataa
     2761 gcaccgccga tatctaatca gctctcgttt tatggaagaa cattatttag ctgcaacaga
     2821 ttgggtgttt tctgacttca agatcaatcc gttttttcga acaaaacgaa gccagcaaaa
     2881 attgtattgt gagtgtggta gagaattaaa agtgcaatac atcgttaaat cgccaaaaac
     2941 cggaaagatc ctaaaactag gcatcaacca ctttgccgat cacctgcatg tctctccgac
     3001 agtggcagct tcgatccatc agggaatgac aaaagttgat ctggccttag acgaattatt
     3061 atggcttaag cagaagaata tcgattttcc tgaaggacta tggcaaaaat actgttttgt
     3121 cttgtaccaa aatcgtcgga tgaaacagcc atatttaccg gatataaaat tagctcagcg
     3181 gctcgcagaa tttcgccaag tagaaatgcc gatttacata gcggattatc aagcattgga
     3241 aaatgaaatc aagaaaataa gtgaacacat caatgggcag tcaaaaaagc gacagataaa
     3301 aaaagaacta tttgacgact ttgcagaaga attagtaaag gatgtagaag aattcttgat
     3361 caactatcgc gcatttttac gtaaagattg gcaatccatc gtatatgaag aagtgccagt
     3421 acatcctaat gcttattttg aaacatttat ttccgtgttg agaaagacaa aacgtcaacg
     3481 aacaccagaa gtcactgctc agatggaata ttttgcgaaa aaccaacgat tcatccaacc
     3541 aaaaatctat ctatttattt ggaagcaata ttgtcgctat ggttttacag aagggttctt
     3601 cgacagcatt cctcggatag tcagaaatgg ttttttaaaa gtattgcgta aagaacgaga
     3661 agctatacaa tctgctgata agaaagaccg tacagtctca aaagaaaaat ggcaactagt
     3721 agtgaaagat atccaatctg gaaatgtgca agagacaatc gacaaatgga aaggaaaaca
     3781 ttatcgtttt actgaagctc agaaacaagc gttggaatat tatcagaagt tagaagaatc
     3841 actgcgtttc aatgatgagg caagaaagta tctgaaggaa ttactataaa aaacatacta
     3901 agattagagg ggaacatcat tgacgaatga tgttctcctc tattttctat ttttagatta
     3961 tagtgaagag tgaaagaaag ttcgaacaaa gccttgagga ctacgagtcc ctatcaaaaa
     4021 ccgaacttct ttactgtcat tttagaatca ggtttacgta tgttggggcc atgagcccgt
     4081 gtataggaaa ttggaatcga aatgaagcag taaagttctt tcaaaactta aaaaggagga
     4141 aaacaaatgc cttacattct tgcattagat gtttcaatgg gacacagcta cagtgtccta
     4201 tacaaagacg acacctgctt atttgaagac gagattgaac acactcaaag aggatttcat
     4261 gcattactcg aagagatcca gcacttgcct gagcgtccat tgattgtttt tgaagcaacg
     4321 gggatttatt ccagaccagt cgaaaagttc tttcaagatc atcacttttc ttactgtttg
     4381 ttaaacccgt tagaggcgaa aaaacagatg gaagaaagca ccttacgcag ttggaaaaca
     4441 gataaatcgg atgcccaccg gctcgcacag actcatctta aaaatcaacg tcagccaaaa
     4501 gaagtacaaa gcaattttta cttagagatg cgcgatttag cccgttttta tcaagaaatt
     4561 gagaagaaaa tcactcgtct gcgcatggat ttacacaact gccttcaatt gacttttcca
     4621 gagcttgaac agttcttttc aaacaggctg accccttacg cattgacatt gatccgtctt
     4681 tttccccatc cagattttgt attggcatct actcggacaa aaataaaaaa taaactgatc
     4741 aacgaaacac ggaaaaaaat ctccgcgaat cgtgccgagc aaaaggcaga tcaaatcatc
     4801 cactatgcac agtgtgccta tccagctgtt gaaaaagaca gtatccactg tcaaaaaacg
     4861 atctattacg ccgaacttct ccaagattta ctcgaacaaa aa
//
LOCUS       DAZULG010000152         4862 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000152 DAZULG010000000
VERSION     DAZULG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.156"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..2217)
                     /locus_tag="ACG9CY_002629"
     CDS             complement(64..2217)
                     /locus_tag="ACG9CY_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFS4939080.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2475..2711
                     /locus_tag="ACG9CY_002630"
     CDS             2475..2711
                     /locus_tag="ACG9CY_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS4939081.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2730..2945
                     /locus_tag="ACG9CY_002631"
     CDS             2730..2945
                     /locus_tag="ACG9CY_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFS4939082.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(3648..4400)
                     /locus_tag="ACG9CY_002632"
     CDS             complement(3648..4400)
                     /locus_tag="ACG9CY_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4939083.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(4415..>4862)
                     /locus_tag="ACG9CY_002633"
     CDS             complement(4415..>4862)
                     /locus_tag="ACG9CY_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4939084.1"
                     /translation="YRYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVP
                     IFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMK
                     KGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT         1622 a          820 c          746 g         1674 t
ORIGIN      
        1 tgtttagtat agatactaga atgttcgaac caattattat tctgcataaa ttgtttccct
       61 ctcctagatt tcccataatt tttgatagat accatcggca aaacgaagtt catcgtgcgt
      121 tcctttttct accaaatgcc cttcatggag aactaaaatt tgatcacatg cttttgctat
      181 tggtaaatga tgcgcaataa aaataatcgt tttttctttt aaattgagga gataattcat
      241 aattgcatgt tctaacaagg tatctaatcc actagttgct tcatctaaaa ttaatatgtc
      301 cggatttttt aataaagccc gtgctaaggc taatcgttgt cgttgccctc ctgaaagatt
      361 atttccacct tcttccaaca atgtctcgta acgtaatggc tgttgattaa taaattcatt
      421 tagttggact atctcacaaa cttctattat ttgttcgaaa cttggtgggc tatctaaacc
      481 gaatataaga ttctcaatga ttgttccact aaagaaaaat gactcttgag gcaaatatgt
      541 cactcgatcg cgtaatatct gaaaaggaat gtctaagtga ttaatgtttc cataatggat
      601 cgatccttct gatgcttcat aaaaacgaac aaataacttt gcaagagttg attttccaga
      661 accactaact ccaactatag ccgttttact gtgcgaagga attgtacatg aaatttcgtt
      721 caacgttggg gccttcatgc tatacgagaa tgaaacgttt tttacagtga tatcttgttg
      781 aaataattgt tcagaaagca tatattcaaa agactggttc gatttttctg aatcaatcgc
      841 gaaaatttca tttaaacgat tatttgcaac ttgagccgtt tgcattttca cttgtaaatt
      901 aataatgttt tgtaaaggat ctgtcaaaaa tactagtaaa gcattgtatg tgataagttg
      961 acctagacta attctattat ccataacaaa ataagaacct agccataaaa ttaatgcact
     1021 gctaattaat tgaatcaaat gtttaattcc ttgttgaata ttatcaagcg taaccgtacg
     1081 aaatgatttt ttcattaatt taacaaattc atggtctata cggttatata ctttctcttc
     1141 accgctatat gctttaattg tttctattcc tttaagactt tctatgatac tagaatttac
     1201 agctgcaccg gctgccattt cttccctgtt cgatttttca tagcttttca cgaaagcgaa
     1261 tattgcaact acataaaaag gcaaagagca tagagtgatt aaaaacaacg tactattctg
     1321 aatagctaac gtaaccccaa caagcaacac cataccaata tctaaaaata ttgataacgt
     1381 agcacttgct aaagcatcaa taatcttact ggcatcaaga aaacgagaaa taatttcccc
     1441 tgattttctc gtagcaaaaa aattcattgg taaagttaac acatgtttga aatactgaag
     1501 cataattgcc atactcattc gctgtcctaa aataataaga agataactac gactatattc
     1561 aaacaatacc cgaaataaat agacagcgat caagccaatg gaaattatat ttaaagttga
     1621 acgtgcttga ttagggatga aaaaatcaag aataccttga aaataatatg agcttcctat
     1681 gccaaaaaac gtaatgaaaa tcgatgctaa aacgatgtgg aatactaatc ctttttgttt
     1741 ccaaattata ggcaaaaaag aacttaatcc agctactttt tctttaatag gaatataagt
     1801 ttctctaggc ataggtaata aaagaatacc tgtccattct ttttgaaacg cttcaatcgt
     1861 ttttctattt tttccattag cgggatctgc aatccatagt acgtttccct tcgttttata
     1921 aattacaacg tagtgcatat agctgttatc aaccaatata tgtgcaatta atggtaagat
     1981 aagatctctt tctttccaga tctcactatc tgcttgaata gccatacaat caaaagacaa
     2041 tttttcaaat gtctttttca atccaaatgc tgaagtaccc tctaaatcag tcccagatag
     2101 gtcccgtaat ttatgaatcg ggatatcagt tttaaaatat tttaaaatca tcgaaaggca
     2161 agcaacacca caatcttttt catcttgttg ctgcacaaaa ggaaatgatt ttatcactta
     2221 tctaacacaa ctccttctaa aaaattttta tagaaaaatt ctcaatatag ctttaataca
     2281 gtgaaaatat tgtctcataa ttccacaaaa actttgaaaa cagttcttga acactaactt
     2341 tttcattcct gaatcaaatt tattttggaa attttactat tcaagaataa acttgataag
     2401 tttcaggaag atttttactg ttaatcaaat acgtatgtta cagtagcacc gtaaaaaata
     2461 ttatttaaag aagcgtgttt aaacatgcaa aatgtaaagg aactaagtat tgtggaaatg
     2521 cacaaactgt tggtggaggc aataatgctt ggggaaaatt ggggcaagtt gtaggtggac
     2581 ttaccacagg ggcagtaggt ggtgcaggtt taggaacagc aatttgcgga ccagcttgcg
     2641 gtgttgttgg agggttatat ggagctgtcg ctggaggtgc tgcagctggc tgggatgctc
     2701 gaaaaaaata aaaaaggaga ataagtagta tgcatattaa aaacacaaaa acaacattta
     2761 tattatcttc agaagagttg aaaaatattc aaggtgggag tgccgtggga gttttaggca
     2821 ctactttttc tggggcaaca gcaggagtaa aactttgttc agccggagga ccttatgcaa
     2881 tagctgcttg tggtgtaggt ggagctttat taggtgctgg attcagcatg tggaccggtg
     2941 cgtaaacaat taaaaaatat caaacctcaa gattggtttg tgtcgattac gaacataggt
     3001 atgttcttta tcttttcaaa agtttggaat ggacagaact ttttactaaa gcttcttgtg
     3061 ctagttttta taattatatt aaatttttat cttttattaa agtcagataa aaattctttg
     3121 gaaatgtaat ggtagagata tttttacaaa aggacttgca gcgctgtagt tggtgctgca
     3181 ggaggaccta tttcggccgt tacaggaggt attggtaatg tacttgtcgg atgtgcatta
     3241 aagcctgttc ctgcaagtta aatttataaa aacaggaaag taattatctc aaatcctatc
     3301 aagtacaatc ataggattgc acttgacagg acaaattttt taactaaaaa atgaaaaaca
     3361 ctttctaaaa attagtagaa tttgccaaat ctttcgcatc ttccaccata catttaattt
     3421 taattcccct ttatggtact taataaaaac aagaaaagaa ctattcattt ggcccttttt
     3481 tggttccaga ccatagaaat attcctccag taactaatat tgctgttatg cacgtaacta
     3541 ataacagcta taaaaattgg tacaacttta ataggattca gaaaaatttg cactagtgaa
     3601 ggaatctatt ttattagaaa aatgcaatct ccctaaagaa aaaaccacta aacagtgctt
     3661 tctttcaact tctccttaat tttttgtgct ttccctaaag taaagtttct ggccaactct
     3721 gaatcaaaat atagtgaacg ttttttaaaa tctatttctt taacgttaaa gatatttact
     3781 aaacacgctc gactaattct tgttaacata ggatatttct tttcaagctc actaattctt
     3841 ccataaaatt cgtactgccc atctttagta cacaatgata atctatgtgg taattttgaa
     3901 gcttctacaa aataaatgtc atttatatca aaagtaaatg tttgagaacc tatggaaaaa
     3961 acaaatgctt gattcgtaac ctgttttgtt gcatctatcc tttcttgcgc taataacaat
     4021 aactcaacaa tctcgtttcg atactcatct agtgtttgat cttttgttac aaatcctaac
     4081 gtttcaactc ttctttttat tgttacgggt aacatctcat catgagtagt tgtaaaaatg
     4141 atttttgctt gaacatcata ttttctgatt acttctgcta attctatacc gttaacttca
     4201 tgatttaaat caatatctaa gaaatatatt ccttgctttg gtcggaattg tttgaggtat
     4261 tttttaactt ctaacggact ttgtgttttc aatactatct taaatacttt atcgtgaaaa
     4321 agaatgaaat tatcaataat tcgttctaac tgatttaatt gtataatttg atcttcacat
     4381 aatattattg gataactcat aaatcctcct tccctcaaaa caacaaaata acttgagtag
     4441 taaattgata cggatcgttt tgatagttca caaacatatt gggattcttt ttattgattt
     4501 cctctatact agataaacct aacccttgat gtcccttttt tgttgttatt ccttttttca
     4561 ttagagtatc tatagagagt agtatctctc tacaactatt cgatatcgaa atttctatct
     4621 gtttatcatc tcggtaaata gctagagaca gttctctatc ttgactctct atagatgctt
     4681 caattgcatt atcgagaata attccaagca ccctaataca gtcaaatatt ggaacaggaa
     4741 cttcaataat agtacttgga cattcgaatt tacaacaaat cttattgtta tttgcttggt
     4801 atagcttcgc tatcaacaaa cttttcaaat attcattttt tatatttttt aaataacgat
     4861 at
//
LOCUS       DAZULG010000153         4805 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000153 DAZULG010000000
VERSION     DAZULG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.157"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ACG9CY_002634"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="ACG9CY_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS4939085.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="ACG9CY_002635"
     CDS             complement(2065..3063)
                     /locus_tag="ACG9CY_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS4939086.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="ACG9CY_002636"
     CDS             complement(3289..3942)
                     /locus_tag="ACG9CY_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS4939087.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ACG9CY_002637"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="ACG9CY_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS4939088.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1413 a         1007 c          745 g         1640 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat ttttttaaaa
     4801 aaatg
//
LOCUS       DAZULG010000154         4802 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000154 DAZULG010000000
VERSION     DAZULG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.158"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..1099)
                     /locus_tag="ACG9CY_002638"
     CDS             complement(137..1099)
                     /locus_tag="ACG9CY_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4939089.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1101..2540)
                     /locus_tag="ACG9CY_002639"
     CDS             complement(1101..2540)
                     /locus_tag="ACG9CY_002639"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS4939090.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2725..4671
                     /locus_tag="ACG9CY_002640"
     CDS             2725..4671
                     /locus_tag="ACG9CY_002640"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS4939091.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1532 a          816 c          782 g         1672 t
ORIGIN      
        1 atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt
       61 tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta
      121 aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat
      181 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
      241 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca atgaaagagg
      301 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      361 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      421 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      481 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      541 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      601 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      661 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      721 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      781 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      841 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      901 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
      961 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
     1021 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
     1081 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
     1141 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1201 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1261 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1321 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1381 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1441 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1501 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1561 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1621 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1681 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1741 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1801 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1861 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1921 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     1981 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     2041 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     2101 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     2161 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2221 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2281 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2341 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2401 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2461 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2521 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2581 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2641 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2701 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2761 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2821 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc
     2881 acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     2941 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     3001 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     3061 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     3121 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3181 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3241 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3301 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3361 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3421 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3481 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3541 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3601 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3661 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3721 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3781 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3841 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3901 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     3961 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     4021 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     4081 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     4141 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4201 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4261 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4321 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4381 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4441 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4501 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4561 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4621 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4681 cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat caaatatttg
     4741 agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta ttagaatttt
     4801 aa
//
LOCUS       DAZULG010000155         4692 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000155 DAZULG010000000
VERSION     DAZULG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.159"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACG9CY_002641"
     CDS             complement(52..522)
                     /locus_tag="ACG9CY_002641"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS4939092.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACG9CY_002642"
     CDS             complement(681..1139)
                     /locus_tag="ACG9CY_002642"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS4939093.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACG9CY_002643"
     CDS             complement(1310..2455)
                     /locus_tag="ACG9CY_002643"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS4939094.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACG9CY_002644"
     CDS             complement(2468..3385)
                     /locus_tag="ACG9CY_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS4939095.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACG9CY_002645"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACG9CY_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS4939096.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="ACG9CY_002646"
     CDS             4477..4647
                     /locus_tag="ACG9CY_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939097.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1471 a          850 c          883 g         1488 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gt
//
LOCUS       DAZULG010000156         4633 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000156 DAZULG010000000
VERSION     DAZULG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.160"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..108
                     /locus_tag="ACG9CY_002647"
     CDS             <1..108
                     /locus_tag="ACG9CY_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HFS4939098.1"
                     /translation="LRLQTVTWIVFGIVMVIGLIIYFGYGYGHSKMNEK"
     gene            complement(209..1405)
                     /locus_tag="ACG9CY_002648"
     CDS             complement(209..1405)
                     /locus_tag="ACG9CY_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS4939099.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(1529..2191)
                     /locus_tag="ACG9CY_002649"
     CDS             complement(1529..2191)
                     /locus_tag="ACG9CY_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS4939100.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            2419..2982
                     /locus_tag="ACG9CY_002650"
     CDS             2419..2982
                     /locus_tag="ACG9CY_002650"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4939101.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2995..3279
                     /gene="macP"
                     /locus_tag="ACG9CY_002651"
     CDS             2995..3279
                     /gene="macP"
                     /locus_tag="ACG9CY_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS4939102.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            3298..3990
                     /locus_tag="ACG9CY_002652"
     CDS             3298..3990
                     /locus_tag="ACG9CY_002652"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS4939103.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            4002..4547
                     /locus_tag="ACG9CY_002653"
     CDS             4002..4547
                     /locus_tag="ACG9CY_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4939104.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
BASE COUNT         1397 a          838 c          976 g         1422 t
ORIGIN      
        1 cttcgtctgc aaactgtgac atggatcgtg ttcggtatcg taatggttat tggactgatc
       61 atttattttg gctatggcta tggacacagc aagatgaatg agaaatagac tccaatggag
      121 aagttagaag aaagataaga aaaacgccca tcaacagttg taataaactg ttgttgggcg
      181 tttgttttag taacaagcga aaggtgatct attgggttaa atccaacagt ttttgtttca
      241 gatcttcttc catgtgtcca agctctactt ctgcatgacg acgtttttct tttccttctt
      301 tttggatacg caatgtctct tggattgttt cgatcaaatc attttgagtg gtttgaaggg
      361 tctcgatatc cacgattcca cgttcatttt ctttcgctgt ttcgatggct gagattttca
      421 gcatttctga atttttcttc aataagtcgt ttgttgtttc agagacttgg cgttgagcag
      481 ttactgcatc tttttgacga agcaaggtca atgcgatcac gacttggttt ttccacagcg
      541 gaatggctgt agcgatagat gcttggatct tttcagccaa ggcctgattt gtgttttgga
      601 tcaaacggat ttgtggtgct tgctggatcg tgatctgtct agctaagcga agatcatgcg
      661 tacgtttatc cagacgatct aggaattgcg tgtaatcatt ggcaatctga acatccatct
      721 gatcgccagt ttcttctgct cttttcatcg cttctggaat gatcgttgtt tgaagttctt
      781 ccattttcaa ttctccagca gcgatataaa tattcagtgc atcaaagtag tctttgtttt
      841 tctggtaaag ctgttctaac atcagattat cttttagcag accgtctttt tctttatcta
      901 atttcactga aatcttgtca atttgtgcac caatcttttg atattttgct gtcacttcat
      961 agatcgattg tttgaccttc ccgaagacac gctggaagat gttcccttct ccggcacgca
     1021 gttcgtcagg attggcttct tggagacgat acatcagttc agttaaggaa tctcctacag
     1081 gaccaatatc ttgtgcttgc acatgattca acatcgattg tgagaattca cttaattttg
     1141 cttgggcagc cgatccgtag ctgatgacag actgtgcatc attagcatcg atttttgctg
     1201 ctagctgttt agcttgttct tggcgttccg ccggtagttt gtcaattaaa cgagcagctg
     1261 tctgttgctc ttggagctga tcaatctccg cttgttgtga agtggtcagc gtatctgttg
     1321 gagttgaaaa cgggttgttc aataaatctt caagtgtatc gttaactggt gtaacatctt
     1381 ttgtttcagt cggtttatga tccataaaaa attcctcctt gttgaatctg ttctcgattc
     1441 ttacatgaat gtctctactt tagaaaaagt actcgaaaca gccagacatc atctgaatgt
     1501 tccgagtctt cttttatgaa caaaagagtt aatccttttg ttctggttca tatttattat
     1561 cacgctttaa gctctgtttc gcaacagata tctctacgtc gatatcatct agatcctcag
     1621 caacgaattt ttgataatct tgtgcgatta aagctgacag ctggtcgatg atctgcgcgc
     1681 tttcttctag tttttcatag gtctgtttgt ttttgatctc atggtcattg atctcattat
     1741 acttatttgt caaatcaact agattcggca gatgcgtata aagaaactga cttgcttcat
     1801 gcagtcggtt tggttctttc accaattctt tgaataaagc tttcgctgct ttgactggat
     1861 catggcggag atcgattgct ttaagtttgg ctgtctgctg catattcatt tgcaattgat
     1921 tgatctcatt ttttgtttga ttcattgttt gtcgaaaaaa tgaaatttca ttcgctgtca
     1981 taccgctttt ttcatagtga gtttcaagtt cgtcagtcag tgcaggaatt tcttcttgtt
     2041 ttcccttttt cctgcgcgaa ccaaagagtc cccacaaaac taaaactaca cctatcaata
     2101 aaagtcctag aacaaaagaa aaactattgt ttcggacaaa gagaaatagc aagaccaatg
     2161 ctagaagcca cggccaattt cgttttatca taataaaccc tccatgtcat ttatcgttga
     2221 gtattgaatt cttttcgtta ttcgattaca tacttatttt agcatacgga ctactagaaa
     2281 gagtatcgga ctaaagaagg attttctata tttagagaaa atctaaggtt tatctatggt
     2341 aaactaataa ggagtttttg agaaaaattg aaaaatgaaa aaaaagcagt tatgattaca
     2401 aagaaagcag gagaagatat gctgaacaat gagcaatttg aagaaaaaac aatcagtcgc
     2461 aaagaaatct atagcggaaa aattattgat gttgctgtag acgaagttcg tttgccagat
     2521 ggaggaactt cgaagagaga actggtattt catcctggcg gtgtcgggat cattgctttt
     2581 gacgaacaag atcgtctgct tcttgtcaaa caatttcgaa aacctttaga aaaagtgatt
     2641 ttggagattc cagctggaaa aatcgatcca ggagaaggac aaaatcccga aatgacagca
     2701 gctagagaat tagaagaaga aactggctat cgtgcgaagt cgctgagcca tcttacttcg
     2761 atgtatcttt caccaggttt tgcaaatgaa gtccttcata tttatcatgc acaaggggta
     2821 gagaaggtcg agaatccttt agcacaagat gaagacgaag tactggagtt gtatcatcta
     2881 actcttgaag aggcgcaaca agcgatgaaa gatcagttga tttgtgatgc aaagacgatt
     2941 tatgcgattc aatactggga actattgacc aaaggaaagt agagggatcg gcttatggcc
     3001 aaaggaccgc ttattacgcg aagcgaactt cgcaagcgac aacaagcaca agcaagcgag
     3061 tcattaaaaa aacagcgaaa agcagaaaca gcttatcagc aagaagaaaa aaagattgcc
     3121 agcttttatc gtaaagaaag caaaaagaat aaaccaatca cgaaaacaag gatcagcgaa
     3181 cgggaaaaga cgaccaaatg gaactctttt ttgatgaaat ctcttattat tgttatccta
     3241 atgttatgtg tggttttttt agcaatcgca tttatataga aagaaggaat taaaaagatg
     3301 aaaatcggta ttatcggcgc aatggaagaa gaagtaaaaa ttttgagaga gaacctaagc
     3361 gaaccattat catgggaacg cgcaggcgct ttatttattt caggttcatt aggtaaccac
     3421 gaagtgatcg tcgtacgttc cggtatagga aaagtcttag catctattac cacaagtctg
     3481 ttgatccaac aatacggcgt gaacatggtg atcaatacag gttcagctgg cggaatcggt
     3541 gaaggcttac aagtaggtga catcgttata tcagacaaag tagcttattt tgatgcagat
     3601 gcaacaggat ttggctacaa cccaggacaa ttaccaggaa tgcctctata ttatgaagca
     3661 agcacatatt tgcgcagtga aatggttaaa gcagcaaaag caacgaattt gaacgcaaaa
     3721 gaaggattga tcgttacagg tgatacattt gtcgactcgc cagataaaat caaagaaatc
     3781 ttaacgaatt tccctgaagc attagcttgc gaaatggaag gagccgctgt tggacaaaca
     3841 gctcgtcagt tcaatatccc atttttgatc gtacgtgcaa tgagcgacac agcagatcac
     3901 tcggcgacac aaagctttga cgaatttatc gaagacgcag gcaaacgatc agcagaaatg
     3961 gtgatcgaat ttgtcaaaca tctcgtataa ggaggactaa gatgaacgca ttgatctcga
     4021 ttgactacac atatgatttt gttgcagagg atggaaagct gactactggg acagcaggac
     4081 aagtaattga aaaaaagctg actgctcata caaagaagtt tattgatcaa aaggattttg
     4141 tcgtttttgc cattgatgcg catgatccaa aagattcctt tcatccggag aaccgcttat
     4201 ttcctccgca taatgtgata ggaacgggtg gacgggaatt gtacggctct ttaaaaagtt
     4261 tataccaaga ctatgagcaa gaggaaaatg tttattggat agacaaacgg cattattccg
     4321 cttttagtgg aacagatcta gacatccgtt tgcgagaacg ccagatcaca gatatttatt
     4381 tgaccggcgt atgtacagat atctgtgtgt tgcatacagc agtcgatgcg tataatctag
     4441 gatacaaact ccatattttc aaagattccg tggcaagttt cgatcccgtt ggacatgaat
     4501 gggcattgag acattttgag tccacactag gcgcagaaat tttataaaaa aacaaagtga
     4561 tttgataaaa taaaaagcac aaaataataa aagaagagca taaaacaaac gactttgttt
     4621 tatgctcttt ttt
//
LOCUS       DAZULG010000157         4598 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000157 DAZULG010000000
VERSION     DAZULG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.161"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..1311)
                     /gene="dltD"
                     /locus_tag="ACG9CY_002654"
     CDS             complement(43..1311)
                     /gene="dltD"
                     /locus_tag="ACG9CY_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS4939105.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            complement(1314..1547)
                     /gene="dltC"
                     /locus_tag="ACG9CY_002655"
     CDS             complement(1314..1547)
                     /gene="dltC"
                     /locus_tag="ACG9CY_002655"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS4939106.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            complement(1592..2797)
                     /gene="dltB"
                     /locus_tag="ACG9CY_002656"
     CDS             complement(1592..2797)
                     /gene="dltB"
                     /locus_tag="ACG9CY_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS4939107.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            complement(2794..4308)
                     /gene="dltA"
                     /locus_tag="ACG9CY_002657"
     CDS             complement(2794..4308)
                     /gene="dltA"
                     /locus_tag="ACG9CY_002657"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS4939108.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            complement(4319..4468)
                     /locus_tag="ACG9CY_002658"
     CDS             complement(4319..4468)
                     /locus_tag="ACG9CY_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS4939109.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
BASE COUNT         1443 a          931 c          766 g         1458 t
ORIGIN      
        1 caaaaataaa gaaaacaagc gagcaacact cgcctgtttt ctttatttta attgtagttt
       61 atctggtatt tgatgttgcc aagatttgct aaaaaaagca tcgtcaagat gatacttact
      121 agcagtaata tgattttctt ctaaaaacgg tctgatctgt tgatctgcag ccaaccagcc
      181 tttccagcct agatggatcg tgtcttccat aaaatagttg gtacccgctt gattgacaaa
      241 atcagctatt cgattgaatc cttggctatt taattgaaat ttgatctttt tggcaaatcc
      301 ttgtaacatt tcttgagata gtccggtata atcgctccat ttctcgttaa ccggtgggat
      361 gatgaacaat acttcgttat ggttttttgc taattgatcc aagaccagct gaaaatcaga
      421 aaattctggc gaaaaacgat aatcccaatt tttttgtgac tgtttgagct ctggctcgtg
      481 ggctcggata cgatgcgtat aaaaatcgtt tttcaacccg aatggattat tcgtcgtttc
      541 tcgttctcca atttgatttg ctaaaatgga taattctgtt tcttggtatt gttttggtaa
      601 tcgttttgac tcatgatcga ttttttgctg tcgatctttc aacccaatat tgctgaatac
      661 ttcatcctct ctcttcagca tgttccattg gctgcgcagc tgattcaaag attcaggtaa
      721 aggtagcttc ccttttttga tcgtttgcag aatcgccgtc aacgtatcat tctcttttac
      781 ttttgagaaa gttaataatc ttctagctaa atagcgatca gcaggcgtta ctttcttcat
      841 tgaaaacaac cagtcgtatg tttgaagttc agaataatac gtattaaagt aatcagtttt
      901 tacaccattt ttaacgaacc actgaggaga aataataaag acgactttct tgtttttcat
      961 agcatctcct gcagaacgca tcatcatata ttggcttaac gattgtgtcc cgggtgctcc
     1021 cagcaaaaat ggacgatagt ttcgctggta tttttctgcc agaacagacg gatgaaatgg
     1081 actgattctg cttaattcac ttgagccaaa aaaaggaata tattgttttt cagataatgc
     1141 ataatttttt atcgcatctc ctttcaacac gttcactgac atggaagaag aggcacgtct
     1201 aatcgtttct ggattagaga tatctacttg ataggaggag gtaaaaaaca caaacagtaa
     1261 gccaacagct aaaacaaccg ggccgaaaat agcaaaaagt ttctttttca tactcaaccc
     1321 ttcaatgcag ttgcttgttg aatgatttta ttcggtgtgc accaatcctc tcgctcgaat
     1381 tcagatacag ggacttggat atccagctct ccgtctagct cgactaataa ttgaacagat
     1441 gctaaagaat caagcaaccc ttcatcaaat agatcaatat cacgattttt tcttacttca
     1501 tctgttcctg ttatttcttc taagattgat aaaatttgtt cttccatttt ccttttctcc
     1561 tttgttatat aaattaatat gtcactattc gttagctaaa aaaaagttta tctaaaaatc
     1621 ctgaaaaaat caggaagcta aaacatactg cttgaaatgt caaaaatacg gctaagccat
     1681 gcgtaaattt gttagaaggc aactgctctt tatgcttctt cttaaatcgg atccatgcat
     1741 ctgtcagaca gatcagacaa gcatgataca gtccatatac gatgtagtac cacgtcaatc
     1801 catgccatat acccattaga agaaacaaag caaaataacc gatatttgac attacgatct
     1861 tgcttttaaa aaattttttc ttcatcatga agaacatcaa tcgcatatat acataatcac
     1921 ggaaccaaaa ggacagcgtc atatgccaac gattccagaa ttctttgata ttccatgata
     1981 aaaaaggttt attgaaattg attggtgtct gatatcccat catgtagctt gtaccgactg
     2041 caaataagct atagccagca aaatcaaaaa acaaatacat actgtacacg tacatataac
     2101 ctactaatgc ccatgaaata ccaccatgct gtaaggcaat gcgtcccact acaggaagca
     2161 gtacttgacc aaaataataa ccgataatga atttatataa aaaaccgaca aaaatatttt
     2221 ggacgccttt tcccaaaaga tcaacatatt tatcacgaac aggcggatgg agctgatctt
     2281 tttcaaatct cctatagcga tcgattggtc cagaagagat tgttggaaag aacagcagaa
     2341 actggatata acgaaatggc ttatatgttt tgatcatgcc atctcttatt tccatgatca
     2401 tttgtacaga tttaaaggtt aaataagaga ttcctaaaaa accaagtaac gatgcttttc
     2461 catgttcaaa aaaaggagtc actttcgtta aaaacagtgg aattatcgct aaaataacgg
     2521 cggcaaaaaa ccatcctgtc tgatttttcc tttgccgata aacaaaatag ccccatacaa
     2581 gtatcgtctg ccacaaaaca tacccaatca gcgccgctcc ctgttgccaa taattacctc
     2641 caaagctgac aaataaaaag aaaagtgtca ctgctgattg ataaactggt aatctttttt
     2701 cccatatcaa taaagataga acgatcggta caagcgcaat ggctaaaaga aaaaaataaa
     2761 acggctctgc ataagggatc atatgaggta acatcattca ggattcacct cagctgttaa
     2821 tttttttcga tcgatttttc cattaactgt ctgaggcagc agatcgacat aatggaactt
     2881 ctgaggaatc atataatcca ttacaacttc tagtaattgt tctttgatag atttagtcaa
     2941 ctgaaaatcc ggctgtttcg ttttgttttc taatacgaca aaagcaacca gctgctgtac
     3001 ttttgttcct tggtattttg gtacgacgac agcttgtttg attcggttgt cttggagcag
     3061 ataatgttcg atatccccga gttcaatccg gtatccatgt aatttgacct gctgatccat
     3121 acgtccttca taaaataaaa gcccatattc atctagtcta cctgcatccc ctgtatggta
     3181 acttgctaag tcattttgac aaaagaacgc agcagctgtc ttttcgggat tgttcaggta
     3241 accttttgat acgcttggtc ctgcaataat gatttcaccc gtttctcctt ttgaaacagg
     3301 tttaccttct tggacaatag aaactttcgt gtcttttttt acgtagccaa tcggtagtcg
     3361 gtcataacta tctagtacat gctggtcaat ctgaatactt gaaatagcta cagttgcttc
     3421 tgttggaccg taagtattat atatccgtgc atttgggaat cgatcaagta gactttctgc
     3481 agtttttttc gtcaattctt ctccgcaaaa taaaaacatg tccaattccg gcacattttc
     3541 tccattgaat gtaggttcca tcaagcaaat atccataaat gatggagtag aaacccacac
     3601 attcaacttc aaggctggca atgtcgcgaa taattgacga aagtcttgaa caactgcttt
     3661 atttaacggc ttcagcattc cgccagaaac taatgcagga taaatagaaa ataccgataa
     3721 atcaaaagaa aatggcgctt gggctaagaa atgcatacca ggcttgatcc caaagtcttc
     3781 catcgtccat tgaacaaagc tgactagatt atcatgactg atttgtacgc cttttggttc
     3841 tccagtagta ccagaagtaa aaataatata aaaattttct gtacctttta cgctatttct
     3901 gggataaaat cctactggta tcttcttgat ttcttgaaat tctttttttg ttatgacagg
     3961 aaccgaagga atatcttcta tccagtcccc gatactgatg accatagatg gtttagccac
     4021 ccgaaagatc gataggatac gctccttggg tgtatgctct tcaatcggca aataggcatg
     4081 gccagatttg acaaccccta gaaatgagac aatcatttca aactccaaat tgccaaaaac
     4141 gacaattggt ccatcgcctt ctaccttttt ttctaaataa taagcaagtg cgtctgatgc
     4201 ttgtttcaat tcaccaaaag tatgcgtctc atccgcctga taagcaactc gatcaggttc
     4261 atttattccc caatcatcga ttgcttcaat aatcgttttg atttccatac tcttcctcct
     4321 aaaattcgtt atagataaaa gttcctcctt gaatattttt atagtgatac agataaatca
     4381 ggaacagcag tactgcacta taaaaaattg ttcgaatgat gaattttccc caataattca
     4441 attttggatt agaacgccat ttgttcattg gattccctcc tcatataacc tcacacaaag
     4501 tgtaactaat tttgacaaaa agaaacatgt gtctagctta cagaaaccgt ttttttacta
     4561 acagttttgt cacatagctt ttataaaaat agcacatc
//
LOCUS       DAZULG010000158         4590 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000158 DAZULG010000000
VERSION     DAZULG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.162"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACG9CY_002659"
     CDS             186..2855
                     /locus_tag="ACG9CY_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS4939110.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACG9CY_002660"
     CDS             complement(2946..3560)
                     /locus_tag="ACG9CY_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4939111.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3679..4218
                     /locus_tag="ACG9CY_002661"
     CDS             3679..4218
                     /locus_tag="ACG9CY_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939112.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4215..4553
                     /locus_tag="ACG9CY_002662"
     CDS             4215..4553
                     /locus_tag="ACG9CY_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFS4939113.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1419 a          790 c          860 g         1521 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa ataacggtgt
     3601 acatttttaa aaggagatgt ataatcgaga tttagaaagc gttttcctca aaatttaatg
     3661 aattaggaag tgattactat gaaaaaatta tttagcgcac tttttattct gttatttggc
     3721 tttattggta ttgggacttc tggaaatata gttaaagcta gcacgatggt tgaacaaaca
     3781 agtgaaatgg caaaaacttt tagtcaatta acaccagaag aacaaagtta cttcaaatcc
     3841 aaaggattta gtgataaaga tactttttac actcaaaaga tagagcctca agatccgaaa
     3901 acacgttttt ctgcaaatgt attgcggtta accgcatcta ctaaaaaaat tactaatacg
     3961 caggggcgga cagaatatat tataacaagt cccaattcgg cgatgtatcg tatttctaca
     4021 cgcttgagtt taggtagtgc aaaatcatat actcaaacag taaatctagc taattcttat
     4081 ggttatggtg gaggaatgta ttttacattt actgggaaaa gacaatatta cagtgtaaaa
     4141 ttagctatgc aagtttataa tacatttggt gtagggtcag tctcttgcac tgcgggagga
     4201 ttgacaatag gaaaatgata atttcttact atgtcttaaa tagaagtgat gatgaaggac
     4261 tcttcgtttt tcaatcatta catgaggata cgatgctaaa agagttttat aatcggatta
     4321 agaataaaca gatagaaaca aatttatatt ttttaagtga ctctatcttg ccttttatta
     4381 gcaaccctaa aattcttaac ttgttgcaga caaaagattt gaaaagttta ccgattattg
     4441 tcattgatga ggaaattgta aaatatggcg cttatctaag cgtagaagaa ttagaaaaat
     4501 taactaacat tggaatgagt attcaggagg ataatggggt acatggaaca tagaatgaat
     4561 atgcacagtt gagaacggaa tttgacattt
//
LOCUS       DAZULG010000159         4573 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000159 DAZULG010000000
VERSION     DAZULG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.163"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..2709
                     /gene="adhE"
                     /locus_tag="ACG9CY_002663"
                     /gene_synonym="adhC"
     CDS             112..2709
                     /gene="adhE"
                     /locus_tag="ACG9CY_002663"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS4939114.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2938..4044
                     /locus_tag="ACG9CY_002664"
     CDS             2938..4044
                     /locus_tag="ACG9CY_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4939115.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4061..>4573
                     /gene="dctP"
                     /locus_tag="ACG9CY_002665"
     CDS             4061..>4573
                     /gene="dctP"
                     /locus_tag="ACG9CY_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HFS4939116.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGP"
BASE COUNT         1549 a          809 c         1001 g         1214 t
ORIGIN      
        1 aaaataactg tgaaaaaaat cacaaaaaag tgtttacata gaaaaataaa tattgtatga
       61 tatacatgtg aattatttaa caaataacca tgacccatta ggaggataat gatggctaaa
      121 gtaatagaaa aagaaaaaac gaagacaatt gacgtacaag caatgattga tgatttggca
      181 gaaaaagcaa atgcagcatt gaaagaaatg gaaagctttg atcaagaaaa agtagaccat
      241 atcgtgcatg aaatggcaat ggcagctttg gatcaacata tgccattagc gaagatggct
      301 gtagaggaaa caggccgcgg tgtttacgaa gataaagcaa tcaaaaatat gtatgcgtcc
      361 gaatatattt ggcataatat caaacatgat aaaacagttg gtgtgatcaa tgaagatgtc
      421 caaaaagggt tgattgaaat tgctgaacca gtcggtgtgg tttgcggagt aactccaaca
      481 acgaatccga cttccacaac gattttcaaa tcaatgatcg cgttgaaaac aagaaatcca
      541 atcgtttttg ctttccatcc aagtgcccaa aaatcttctg cagaagcagc acgagtggta
      601 cgtgatgctg ctatcgctgc cggagctcct gaaaattgta tccaatggat cgaacatcct
      661 tcgatcgaag cgacttctat gctaatgaac catccaggta tcgcgatcgt cttagcgaca
      721 ggtggagctg gaatggtcaa gtctgcttac tctacaggaa aaccagcttt aggtgtaggt
      781 ccaggtaacg taccagctta tattgaaaaa acagcaaaaa ttaaacgtgc agtgaacgat
      841 ttgatcgttt cgaaaacatt tgataatgga atgatttgtg cttctgaaca agctgtgatc
      901 gtggataaag aaatctatgc agcagtaaaa gcagaattcc aagcacacca agtttatatc
      961 gtaaaaccag atgaattgca aaaattagaa gatgcagtga tgaacgaagg aaaatatgcg
     1021 gtcaatccat caatcgttgg acattctgcg atggaaatcg caaaattggc aggaatcagt
     1081 gtaccaaaag gaacgaaaat gctgattgct gaattggaag gcgtaggccc agattatccg
     1141 ctatcaagag aaaaactttc tccagttctt gccatgatca aagctaacaa cacggaccat
     1201 gcgtttgatt tgtgtgaagg tatgttgaat cttggaggat taggacatac agcagttatc
     1261 cattcagaaa acgaagagtt gcatgtcaaa ttcggtcttc gaatgaaagc ttgtcgtatt
     1321 ttagtgaata caccatctgc agaaggcggc atcggtgata tctataacga aatgattccg
     1381 tcattgactt tgggatgcgg ttcttatggt aagaactctg tttctcgtaa cgtctctgct
     1441 gtaaacttaa tcaatgtcaa aactgtagcg aaacggagaa ataacatgca atggttcaaa
     1501 ttaccgccaa aaatcttctt tgaaaagaac tctctattgt acttagagaa aatggaaaac
     1561 gttgaacgtg tcatgatcgt ttgtgaccct ggtatggtcc aattcggtta ctgcgataca
     1621 gttcgtgaag tattggcacg acgtaaaaat gatgtgaaga tcgaagtctt ctcagatgtt
     1681 gaaccgaacc catcgacaaa taccgtatac gcaggaacga aactgatggc agacttcaag
     1741 ccagatacag taatcgctct tggaggcggt tctgcaatgg acgcagctaa gggaatgtgg
     1801 atgttctatg aacacccaga cacatctttc tttggtgcaa aacaaaaatt cttggatatc
     1861 cgtaaacgga catataagat cgacaaacca gaaaaaacac aattcgtatg tatcccgaca
     1921 acatcaggga ctggttctga agtaacgcca tttgcggtca tcactgacag tgaaacacat
     1981 gtgaaatatc cgttagcaga ttatgcattg acgccagatg tcgcaatcgt cgatcctcaa
     2041 ttcgtcatgt cagttccagc atctgtcaca gcagataccg ggatggatgt gttaacacat
     2101 gcaatcgaat cctatgtttc agtcatggct tcagattata cacgtggatt gagcctacaa
     2161 gcaatcaagt tagtctttga ctatttagaa aaatcagtaa aaacacctga tatggaatca
     2221 cgtgaaaaaa tgcacaacgc ttctacaatg gctggtatgg cgtttgctaa cgcattccta
     2281 ggtatttgtc actcagtcgc acataaaatc ggcggggaat acgggatccc tcatggacgg
     2341 acaaatgcga ttcttttacc gcatattatc cgctataatg caaaagatcc acaaaaacat
     2401 gcgatgttcc ctaaatatga ctatttccgt gcagatacag attacgcaga tatcgcaaaa
     2461 ttcttaggtc tgaaaggcga aacaacagag gaattagtcg aagctttagc aacggctgtt
     2521 tatgaattag gaaaatctgt cggcatcaac atgagtctaa aagctcaagg agtcacacaa
     2581 gaaacactag atacaaccgt agaccgtatg gctgaattag cttacgaaga ccaatgtacg
     2641 acagctaatc cgaaagaacc attgatctct gaattgaaac agatcatcat tgatgcttac
     2701 aacggataag caaaagaaag cgtagaaaaa agataagaaa atagatgaaa aagaacttgg
     2761 aaaacgttgt ttctccaagt tcttttttta atttccaagt aactgaaagt tacttttgtt
     2821 atttaacatt taggtcaatt ttagacagtt tttgttcaaa aagcgatatt tatagtggac
     2881 ggtattactg ctatattaag gatataaaat gaaagcgttt aaaaaggtgg cgtaaagatg
     2941 attattaaag aagagatttt tgtacctaaa gaattagtga aagaaaaaat tgacctgctc
     3001 gttgaaaacc tgacaacgat caaagacgat aatggtgaat ttttgttgga ctttgatgga
     3061 ctgaaagtag atgacaaaag ctggacagtg tggaattggc cacaaggtgt cggtctatat
     3121 ggtatttata aaaattacca gatgacaaaa aatcctcgcg cttatcaagt ggtcaatgaa
     3181 tggtttgagg atcgtatgga agaaggcgct ccgcccaaaa acgtcaatac aatggcaccg
     3241 ctattgacta tggcttactt gtatgaagac acgaaagatt cacgctattt gccttattta
     3301 gaacaatggg cagaatgggt catgaatgac atgcctcgga caaatgaaga cgggttacag
     3361 catgccacat atggaccgga aaataagaat cagttgtggg acgatacatt gatgatgaca
     3421 gtgcttcctt tggcaaaaat cgggaaactg ttgaatcgcc cagaatattt agaagaagct
     3481 cgttaccagt ttatgatcca tatcaaatac ttgatggata aacgaacagg attgtggtat
     3541 cacggctgga cgtttgaagg aaatcataac tatgccgaag cattttgggc tcgtggaaac
     3601 tgctggttga cgattgctat tccagagatc attgaaatct tagaattatc gaaagaagat
     3661 gcattgcgca aattattgat cgaaacactt gaagcacaag tgcgtgcatt aaaaacttat
     3721 cagtctgaaa gcggtttatg gcatacgttg ttggatgatc cttcttctta cgtagaatct
     3781 tctgcaactg caggatttgc ttacggtatt ttgaaagcgg tccataaacg ttatttacca
     3841 caagaatata aagaagtcgc ttatcgtgct atccaaggat tgcttgaaca aatcgatgaa
     3901 aaaggagaag ttcaaaacgt ttcaatcggg acaggaatgg gggacagttt agatttttat
     3961 cggaatatcg gaatcacagc aatgccttat ggacagtcgt taacggtgct ttgtttgaca
     4021 gaattgctcg tctcttattg ttaaaagaaa ggaaacccat atgaaaaaaa catcactgct
     4081 gtttgcagca agttcgctta gcctaggtct gctaggtcta agcggctgcg gaaataattc
     4141 tagtgtggca actgtcgcag atgcagatgt aaatgagata gttaccttgc gctttgctta
     4201 tgcaagcaac agccagccag taattgatgc aatgaatgaa tttgggcgtt tagtaaaaga
     4261 aaaaacagat ggttccgtac agatccagta tttcccagat ggacaactgg gtggagaacg
     4321 tgaactgatc gaattgacac agtcaggagc agtagatttc acaaaagtaa gtgcctcagc
     4381 gttggaaagt ttctctaaag attattcgat tttttcagtt ccttatctat tcaatgatga
     4441 agaacatttc tttaaggtga tggataatga agaaataatg gagccaatct accaatcgac
     4501 caaaaattta ggatttaccg gattgaccta ttatgattct ggtcagcgca gcttttatat
     4561 ggtagacggt ccg
//
LOCUS       DAZULG010000160         4417 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000160 DAZULG010000000
VERSION     DAZULG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.164"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..914
                     /locus_tag="ACG9CY_002666"
     CDS             60..914
                     /locus_tag="ACG9CY_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4939117.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1011..3260
                     /locus_tag="ACG9CY_002667"
     CDS             1011..3260
                     /locus_tag="ACG9CY_002667"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4939118.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3263..>4417
                     /locus_tag="ACG9CY_002668"
     CDS             3263..>4417
                     /locus_tag="ACG9CY_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4939119.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          806 g         1430 t
ORIGIN      
        1 tgtaataata taaacataat aaaacaaaaa ttatccaaat ctaacatagg agggtggata
       61 tggaaaattc tatatattta gtaacagaac ctaacactca gctgaaagat tgttacttta
      121 atttttgtgg gtattcaaaa acagaaccta gacatagttt tggaccagct attcgagaac
      181 aatacttaat acacatagtg ctagaaggtg aaggctatta ttcaattaat gatcaaaaat
      241 attttctgaa gaaaggccaa ggatttgtta tcccaccagg agtatctact ttttatcaag
      301 cagatgaaaa aaatccgtgg tcatatgtat ggttgggttt aagtggcaat cttgtagaat
      361 actatctaaa agaaattggt gttggaaaga atcatcttac ctttaaagtc gaaaatttaa
      421 attcatttaa aggattaatt tttgaaactt tatcgtatga tcaggacaat ctagtcagtg
      481 aattgtccag acaaaaacaa gtttataaat ttttagaact cctcttaaat tcagctagtt
      541 atctgaacga agggataatt actaaaaaga tgaatccttt tgttcgtcaa acaattgaaa
      601 taatacgtga gaatgcgtat caaaaattat ctgttaaaga tattgctgtc agtctatcga
      661 tacacgaaaa ttatttatca cgattgttta agaatgatat gggaatatca ataaaagagt
      721 atattagtga aatcaaaatt tctattgcaa acgatctgtt atcatcaact gaatattcaa
      781 tccagcaaat cagtgacaag ataggattct caacagtaca atctttttct aaagccttta
      841 agaaagcaaa tggtgtttca ccgggagtat atcgaaaacg tagattaggt ctaggaatca
      901 ttaatggtga ataaactaaa catttgattt tgataaatat tcgttacttt aaacgcttac
      961 tatttatgtg agcgtttttt ataatgcaat aaagaaacgg aggagagctt atgtataagg
     1021 ataatgaaat gaatattatt tatcgatcag aaaaccaaca atttcatttg tttaatcagg
     1081 agataagtta catttttaag atatcggaag atggtaaatt attgcattta tattatggtg
     1141 agaacttacc agataatgac tatagtcatc taattgagat gcatcatcgg cctatgacaa
     1201 cctatagaaa tgaaaacgat ttgctatatt cgttagagca cttaaaacaa gaatttcctg
     1261 aatatggtac aacagatttt agacatcctg ctatttcatt actacagaaa aatggctctc
     1321 gcttatcaga ttttgtctat caagggtacg aagtggaaga aggaaaacct aaactagaag
     1381 gtttacctgc tacctatgtt gataataatg atgaaagcaa aacattgaaa gtttatattt
     1441 tagattcagt tacaaatgtt caagtagaat tattatatac aatttataga gattatcctg
     1501 ttattactcg tagcgcgcga gttatgaata aaggaaaaga tattcaaata atcgagaata
     1561 ttagcagttt atcattggat ttacccgatg caaattatga atggcaacaa ttatctggtg
     1621 cttggggtag agaaagaact attaagtcgc gtactttaca acaaggaatt caatcaatcg
     1681 aatctactcg aggtatttca agccatcagc ataacccatt tgttacttta aaaagaaaaa
     1741 atactgatga aaatcaagga gaagctattg gtgcagcact agtttactca ggaaacttct
     1801 taattcaagc agaagtagat acatgggatg ttactagact acaagttggc atcaatccat
     1861 ttggatttaa ttggaaactc aatccaggag aaatatttac agcaccagaa gcgattctgg
     1921 tttattcaag taagggttta aatgccatga gtcaaacttt tcatcaacta tttagaaaac
     1981 gcttagctcg tggtaagtgg cgagaaaaag accgtcctgt cttaattaat aattgggaag
     2041 caacatattt tgattttgac gaagaaaagt tggtaaatat tgctaaaaaa gcatttgatg
     2101 ttggtattga attatttgta ttagatgatg gctggtttgg agaaagagaa aacgattgtg
     2161 caggattagg agattggcat gtaaatccta aaagactacc tagaggaatc ggcagccttt
     2221 cagaaaaaat tcgtagtttt ggtatgaaat ttggtttatg gtttgaacct gaaatggtga
     2281 ataaggatag tgagttatat cgcacacatc cgaattggat tatttctaca cctaatcgaa
     2341 aacctaatca tggtaggaac caatatgtat taaattttgg gttagatgag gttgttgaaa
     2401 atattttcga tcaaatgtgt aaaatcattg atgaatcaaa cttagattac attaagtggg
     2461 atatgaatcg tccattgact gatgtatttg acagtcactt gtcagcagat caacaaggag
     2521 aagtattcca cagatatgta ctaggcgtat atcgcttata tgaaaaactt ataacaaaat
     2581 atcctaacat actatttgag tcttgttcat caggtggtgg gcgctttgat cctggaatgc
     2641 ttcactatgc acctcaagct tgggctagtg atgattcaga tgcaattgaa aggttaaaaa
     2701 ttcaatatgg aactagtatg ctgtatcctc tttcgtctat tggtgctcat gtatcaattg
     2761 ttccaaatca tcaaacaaac cgtataacac ctataaaaac tagaggaaat gtagcttttt
     2821 ttggtgcatt tggatatgag cttgacttaa atgaattaag tgaaggcgat ttgaggattg
     2881 taaaagaaca aattgccttt tataaaaaat atagaaccat aattcataat ggtacttttt
     2941 atagactatt atcaccattc gatgatgata atcaaaccgc gtggatggtt gttagtcatg
     3001 ataagaaaac ggctattgtt gctcattata aaacattaaa tgaggtaaat gctccttacc
     3061 gccgactgaa attaaaaggg ttagataatg acggacttta taatgtagga caaaatagtt
     3121 tgcgatctgg aatggaattg atgagagcag gactagtctg ttcagatgca tctagtggac
     3181 aaataaatga tcagaatcga cgcccagaaa catttgattt tgattccaag atttgggtta
     3241 taacacagga ggaaaagtag agatgaataa ccaaaaaagc ttacttcaaa aaggagcaat
     3301 cttaagtatt tcactaatgt taacaagctc tcaagcaatt aatggtgtta ttccacaaat
     3361 gaaacagtct ttggggatat cacaaagtca aagtgaactt ttaggaactg caccaagtat
     3421 aactgttatt ttgtttatct tattatctag ttactttgcc aaaagaatag gaatgaaaaa
     3481 gacaataatt ttaggtttat tattagctgg tataggagga attattccag tatttacagc
     3541 taccttccaa accgttctta ttagtcgagt aatattaggt gccggtttag gagtatataa
     3601 ttcattggct gtgacatata tcagctcttt atactctggc aatactagag caacgctatt
     3661 aggtattaga aattcaatgg aagctttagg tcagacaatc cttatttttc ttgctggagt
     3721 tttagtgaac attaattgga cggcttcgtt tttagtctat gcaattgcgt ttcctgtagc
     3781 tctcttattc gcgctaaaag ttcctgaaat tagagatgaa aatagtgaca tacctgaaaa
     3841 tcatgtaaaa gaaaagatga atccaatggt ttttgcccta gttctatttg caatcttact
     3901 cgtaatgaat agtattgcta tttctgtacg atttgcatca attgcaactg aaattaaagg
     3961 ggtaaacttt aatgctagta actatttagc tctaatgcct atcttaggaa tactagcagg
     4021 atttatgttt ggaccaataa ataagtggat aggaaaagga accctatact tgggaattat
     4081 attctttata atttcaaatt tgttaattgc tgtgtcaaac ggtaatatga cgttcctgct
     4141 aacgggttta tttttatcaa gtataacagg atcatggtgc ttcccattta tctttagtaa
     4201 cttagataaa gtcactacaa agaatacaat taattttgct acttcattga tttttattgg
     4261 ctgtaatatt ggtaatttta tagctcctat tgctatgcaa ttagcacaat ttctaacaag
     4321 aagtacaaat ttgactgctc cattttttgt attcgcagga atatttattg tgatcctatt
     4381 tgtaacattt tttgcgacaa ataaaaaaaa tactaga
//
LOCUS       DAZULG010000161         4384 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000161 DAZULG010000000
VERSION     DAZULG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.165"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1303
                     /gene="maeA"
                     /locus_tag="ACG9CY_002669"
     CDS             <1..1303
                     /gene="maeA"
                     /locus_tag="ACG9CY_002669"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HFS4939120.1"
                     /translation="FLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNG
                     VDIAIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEF
                     IDQFVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLG
                     GLNISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLF
                     EDTKGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKE
                     MAAHTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQA
                     NNALMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQ
                     TIAETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            1457..2779
                     /locus_tag="ACG9CY_002670"
     CDS             1457..2779
                     /locus_tag="ACG9CY_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS4939121.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(2841..3272)
                     /locus_tag="ACG9CY_002671"
     CDS             complement(2841..3272)
                     /locus_tag="ACG9CY_002671"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4939122.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3336..4172)
                     /locus_tag="ACG9CY_002672"
     CDS             complement(3336..4172)
                     /locus_tag="ACG9CY_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS4939123.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
BASE COUNT         1331 a          881 c          836 g         1336 t
ORIGIN      
        1 atttctttca atcgacgaac cagaaaatat caaagcaagt ttgaaaaacg cagcagatgg
       61 acgcgacatt cgtttgatcg ttgtgacaga tgcagaagga atcttaggta tgggtgactg
      121 gggtgtgaac ggtgttgaca tcgctatcgg taaattgatg gtctacactg ccgcggctgg
      181 tatcaatcct gcacaagtct tgcctgtatc aatcgacgct ggtactaata acgaagaatt
      241 attgaataat ccattatact tagggaatcg tcatgctcgt gtagaaggcg aaacttacta
      301 cgaattcatt gatcaattcg ttcaatcagc tactgaatta ttcccagaat tgttattaca
      361 ttgggaagat tttggacgag gaaatgctgc aacgatctta gaaaaatacg aagataaaat
      421 cactactttc aatgatgata tccaaggcac aggaatcgtt gtactagctg gtgtattagg
      481 tggattgaat atatcaggtg agcaattgaa agatcaaacg atcttgacat ttggtgctgg
      541 tactgctggt gtaggtatcg caaacatcct tttagatgaa atgatccgtc aaggtgtacc
      601 tgaagaagaa gcacgccaac atttctatca agtagataaa cagggtttat tgtttgaaga
      661 tacaaaagga ttgactcctg gtcaaatccc attcgctcgt aaacgctcag aattcactaa
      721 tgctgatgaa ttgacaaact tagaagcagt tgtaaaagca atccatccaa cgatcatgat
      781 cggtacttct acccaacctg gagcattcac tgaagaaatc gtgaaagaaa tggcggctca
      841 taccccacgt ccaatcatca tgcctttgtc taatccaaca aaattggcag aagcaaaagc
      901 aaaagatttg attgaatgga cggacggaaa agctttagtt ggaacaggta tccctgctgc
      961 tgatgtggaa tataacggtg taacttacca aatcggtcaa gcaaataatg cgttgatgta
     1021 cccaggttta gggcttggtt tgattgcttc aacagctaca cgagtaaatg ctgaaatcat
     1081 ttctcaagct agtcgagcat taggcggaat cgttgatgta acaaaaccag gagcagctat
     1141 cttgcctcca gtagcaaaaa tcactgaatt ctctcaaacg attgctgaaa ctgtcgcaaa
     1201 atcagttgtt gcacaaaaat tgaatcgtga agaaatcacg gatatcaaag aagctgttga
     1261 atctgctaaa tgggttcctg aatataaatc tttagaagat taagattgtt atagtaaaag
     1321 aacatacaga aactttgtat gacgtctaaa aagaaaaaag cgactgcggc aaaacagcag
     1381 ccagccaact actgctcttt tgccgcttct ctttggcttt tttcatacga tacagaataa
     1441 gaaaggatct gtaaaaatgg cacaaaaaac aacaaccgca actgcagttg aggaaaagaa
     1501 aaaagaaggg ttttggtcaa caaaaattac cggcgtaagt ttgccccttt atttgatcat
     1561 ggtccttgtc ctgatcattg caatggcttt aggaaaatta ccgacgaata tgattggccc
     1621 aatcgctgtt ttggtcattt tcggtaattt atttcatttt atcggaacaa aaatccctat
     1681 agtcaaaagt tatttaggtg gtggctccgt ttttgcgatc tttgcctctg ccgcgattgc
     1741 tacatttggg atcttacctg agtatgtagt aaaatccaca gaaaactttg tcagcaatat
     1801 gggcttttta gacttttata ttgctgcctt gattactggt agcatccttg gtatgaaccg
     1861 taatctattg atgaaagctt ctgttcgttt tattcccgtt gcacttattt caatggtcgt
     1921 ctcctttttt gccgtaggac tcgtaggtat gcttatcgga aatggttttg caaattccgt
     1981 tttatatatc tcattgcccg caatggctgg tggtgttgga gccggagctg tacctttatc
     2041 aacgatttat gcaaatgttt taggtacgga cgcttcttct atcatttccc gcctgattcc
     2101 tgcgacagca atgacaaatg tcctagctat cattggtgca gcgttagttg ctcgtgcggg
     2161 acaatcaatg ccaaaattca acggtaatgg taaattaatg gaaaaaggcg accttggcga
     2221 tggaaaacct agaaatgtaa aaccttccat tccacaatta ggtgtcggtt tgatggtatc
     2281 attgactttc ttcatcttag gacaaatctg taactatttt gttcctaaag tccatgctta
     2341 cgcattcatg atcattatcg ttgttatctg taaaatcgca aacctattac cagaatatta
     2401 cgaagatgca gctatcatgt tcaacaactt gattgtcaaa aacttgacag cagctgttct
     2461 tgccggtatc ggtattgcgt tattgaatct aaatgtattg gcttctgctt tgacatggca
     2521 atttgttgtc ttatgtttga caagtgttat cgcgatttca gtagtttcag gatttgtcgg
     2581 tcgtttgttt ggtttatatc caatcgaatc aactattact gctggcttat gcaacaactc
     2641 gatgggtggt acaggaaatg tagctgtctt atctgcagct aatcggatgg aacttattgc
     2701 ttttgctcaa atgggcaatc ggcttggtgg tgccatcgtt ttgatcatct caggtttctt
     2761 gatgcaatta ctttcttagt caaaaaaaat acctgatcat caagtatcgg cttgatgacc
     2821 aggtattttt tgataaaaat ttacaggatt ttcaaaaatc ttttttgcat tttatcggaa
     2881 tgataaccga tgcgttcata aaatcggtgt gcttctactc tgtattctgc agaattaagt
     2941 cttattgctg atattcctcg tcttttcgct tcttctacgc cagtcatcaa ggcctttccg
     3001 attcctcttt tttcagcatg tttagacaca gcaagtgcca taatattgaa catcggatcg
     3061 ctgaaaatcg attcatagga ctccgcttgt acatagccag ctgctttttt agaagtagga
     3121 tcttcataga ccaagaaaaa atttttttct ggctccttca acagtttctt catttgtctc
     3181 accgtaacgg ttaatggtac atcatatcct aattgttccg cattgatttc tcgtagtgct
     3241 tctgcatctc ctaaacgcat ttcccgcaac atccaaatca ccagccaatt tttatttttt
     3301 taaatgaatt tttttatcac agtatactcc ttttttcact taaaaaaaga cttttcagtg
     3361 attttgactt gcttgaatcc agatccttct aatgtttgca agatgacgaa agcattgtaa
     3421 agattgatca aaagaaccac gatgacaatc gcttgaaaaa taaaggtcac tttttttgga
     3481 gacaaaaccc tgctaaatct tgctccccac aaacctccaa taacagcagc ggggataaca
     3541 taaaacaaca tagataaatc aaagcgcata aagctacttg ttaaagcaat cgttaccaat
     3601 tttgccagtt gagaaaagaa aatcgtagat agagaatata gtgttgcttc ttttattggc
     3661 aatgcaaaca tcagcatcaa caaagaaaca ttgactggtc caccaccaat acctaaaaaa
     3721 ctagctaaaa agcccagaac ccccccacat ataaggtacc aagtccatga cgtcaaatga
     3781 aattttggct gatgatgctt ggtgtagaag aacgcaaaaa ccaaggtcaa taccgtcaaa
     3841 aaaatttgga tcagctgcac atgcttctcg ttttcaaata actgtaaaaa aacttcgaat
     3901 acaatgttgc cgactactcc tccaacaaca gctccactag atacccaacc gacaatctgc
     3961 caattcagtg atttcccgct tgatacttgt cgcacagtcg aaacaatgga catcgtgaat
     4021 accgcaactg ttgagtaaaa agaaatccct gcaacagaat gggcgccgat aagatcaaga
     4081 atcggtttga tcaacacgcc tccacccatc ccggaaatag cgccaactgt gttcgccaga
     4141 acgatcacca caaaataaat cagaccaatc atcttgtatt tcctttctct tgtttatatc
     4201 ttttagcgat tgttactctt ttcttttatt atacagtaaa agaaacaaac gaggcttcga
     4261 aagcttttct actttttaag gaaatttagt gctaaacgaa aaaaccgggc tctacttttc
     4321 agagagtcca gcttttctta caccttatcg attcttttgt tctattcttc ttccgccatg
     4381 aatg
//
LOCUS       DAZULG010000162         4255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000162 DAZULG010000000
VERSION     DAZULG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.166"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1348
                     /locus_tag="ACG9CY_002673"
     CDS             47..1348
                     /locus_tag="ACG9CY_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4939124.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1375..1635
                     /locus_tag="ACG9CY_002674"
     CDS             1375..1635
                     /locus_tag="ACG9CY_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4939125.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2634..3869)
                     /locus_tag="ACG9CY_002675"
     CDS             complement(2634..3869)
                     /locus_tag="ACG9CY_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4939126.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1232 a          682 c          880 g         1461 t
ORIGIN      
        1 gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat
       61 gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt
      121 attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt
      181 tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc
      241 aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt
      301 tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc
      361 tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc
      421 tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt
      481 atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa
      541 aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc
      601 agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc
      661 cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt
      721 actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt
      781 aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc
      841 agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac
      901 ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg
      961 taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat
     1021 caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt
     1081 caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt
     1141 caatagattt actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc
     1201 agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat
     1261 tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg
     1321 tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat
     1381 tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat
     1441 atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg
     1501 atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca
     1561 tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc
     1621 acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat
     1681 aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt
     1741 ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga
     1801 ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt
     1861 tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt agaattagta
     1921 gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag
     1981 gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg
     2041 aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt
     2101 agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt
     2161 gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg
     2221 gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg
     2281 gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga
     2341 aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc
     2401 agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt
     2461 ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta
     2521 agtaactcta acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc
     2581 gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt
     2641 gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga
     2701 aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga
     2761 gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt
     2821 acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg
     2881 ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg
     2941 aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat
     3001 tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt
     3061 aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag
     3121 ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag
     3181 gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct
     3241 tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa
     3301 cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc
     3361 catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg
     3421 aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa
     3481 gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc
     3541 acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta
     3601 gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca
     3661 aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca
     3721 aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa
     3781 taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat
     3841 actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc
     3901 ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa
     3961 tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagttga
     4021 aactccaatt ttcaaaaaaa cacgcaacaa ttttttgatc gatttatatg ttgtgccaga
     4081 taaatacgag aagttgtgtc attaaaaata aacatgagaa cataaaaaaa taaaataatt
     4141 atattaattg ataggtgtgc tgtcgtcatt atacacacgt aagaaagaaa tagttatgtt
     4201 taaaaatatt cttagacttt ctcgccatga caattttcac tatgtgttat aactg
//
LOCUS       DAZULG010000163         4240 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000163 DAZULG010000000
VERSION     DAZULG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.167"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..476
                     /locus_tag="ACG9CY_002676"
     CDS             39..476
                     /locus_tag="ACG9CY_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939127.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            571..1455
                     /locus_tag="ACG9CY_002677"
     CDS             571..1455
                     /locus_tag="ACG9CY_002677"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4939128.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1677..>4240
                     /locus_tag="ACG9CY_002678"
     CDS             1677..>4240
                     /locus_tag="ACG9CY_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS4939129.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIP"
BASE COUNT         1386 a          713 c          959 g         1182 t
ORIGIN      
        1 agaaaacgat attaaaaaat gcacgtagaa gtagataaat ggagtggttt tgcaaatttc
       61 ttttatttct agaagttatt cacgtatacg gatttgtttt tctcacaaat aaaaaggcag
      121 gaagtacaat ggatcatttg tatataaaat atattcatca attttccgtt ttggtattag
      181 gcacactaat tatagcgggg attgttgcat ttattattca aggtcgtgac atattaacag
      241 tacatcaaga gtttttcagt tatgcatttg gtattcctct gatcgctata aggtctgata
      301 acaaaaagtg gaagaaacgc tattacattc tcttgctatt tttgtgtttt tatctgccac
      361 ctaagatttt tactttatta ggtatacaat gcctatcatt ttcttttatt tcctatatca
      421 ttgttttaat tttcctcagt ttcatagtat gggtatttta tcttgtcaaa aaataaatga
      481 atgtttcatg aacagtactt catcacagcc taattttttt ctttatgcta tcgttatatt
      541 aaagaaatca atcagaaggg aaagaaagtg atgaaaaaag ttttattagt ggtaaacccc
      601 agttcaggcg gtgaacaagc aaaggaattt gagcaattag cgatagcaaa attggaatcg
      661 gtgtttgatg aagtggtcgt cttgcataca aaaaaagcag gggatgcaaa aaactttact
      721 cgcgaagcgg ctacggaagg gtatcatagt gtgtttgtca tgggcggaga tggaacagtc
      781 aacgaaggaa tcagtggaat cgctgaacaa gaacatcggc caaatttcgg atttttccca
      841 ttgggtacgg tgaatgactt ggctcgagca ttagggatac cgctagaacc tgaggaagcg
      901 atcaatcatt tttcgattga atcagtcaag ccattggata ttggaaaaat aaatgacgac
      961 tattttatga atgtcgtcgc aattggctct atccctgaag caatcaatga tgtagatgcg
     1021 gagaagaaga cgaaatttgg aaaactagct tatttcatgt caggaatcaa acagttagct
     1081 tccacacaat cctactcgtt tcacgtagaa gttgatggaa agaaggaaga aattgagagc
     1141 agtatcttgc tgataggact aactaactca gtaggcggtt ttgaaacatt acttccaaat
     1201 gcaaaagtgg atgatggtaa gctccatttg gtttatttaa aagattcttc tttgatcgat
     1261 acgcttaaaa cattgccaga cctattaaaa ggagtagatg aatctacgaa aaatctagcc
     1321 tatcttactt gtgagcaatt agttgtgtca ttagctgatg aggaagaatt gacgacgaat
     1381 gttgatggag atgaagggga taaattacct gtgacaatca aagtgttacc ctcacattta
     1441 caggtttatt gttaaaatgg aggttgtgac cgaagagttg tcaggaggac atcggttgtt
     1501 cggaataagg gactgtgaca agacttttgt cacagccttt ttttgtacaa aaaagaaatt
     1561 ttcacctaac agaaaaattt gccataaaaa tacatatttt aaatatgaaa gtacaattac
     1621 aaataaatta aaacgttaaa ataaaattgt caaaacgtca gagaggatgg tggagaatga
     1681 atcaatatgt aaaaaagctg attgcgtcga ttttgtttgt cttccctctc ttgatggtga
     1741 catccgtttt tgccgatgta gagaaaacaa gcgaatcaac gcaatcagag aatcaaagtt
     1801 ttgacaagac tactttttca gaagaatcaa ttacttctga aactacagaa tcccaaagtg
     1861 aggcgcctac ttctaataca aggcaagaag aaaataagac aagtgagacc tcagaagtag
     1921 ctgaacaaac gtctgatagc agtgaagagg tacaggttag agttgtttca gcggatgggt
     1981 acagcgaagc tgccacaaca gaagaactga cgcagcttct cgcagacgaa tcagtagcta
     2041 agattcgttt aactcagcct ttgactttag acagggaatt ggagatcaag cgagatatcg
     2101 ttatcgattt tggcggactt gctcataatt tcggcacaca tcacatctat atcaatgaga
     2161 cacctcagtc gatcgagttt cagaatttta aaggaacggc agctcatcca ggtgggttga
     2221 taccaaatat cgatggaaat gccattatca ttgcttatat gtccgatttt tggggaaaca
     2281 gatatcattt tacgggagaa ataaaattta ccgggacact tgacttatat gatggttcta
     2341 aattaggcct gatttacgcc cctcgtgcaa ctgtcacatt agatggtgtt tccggtgtat
     2401 tggatgtcca accagtgaaa gaagggatga atgccgctcc gggtaaagct tatttttgta
     2461 gaagctatca gctaaatgta atcaatggta gtcagcttta tggaccatat ctaggaaaat
     2521 tctatggttt tctagattca ggtgatgaga cgggcagtgg aaaaacagca ccaggactcc
     2581 atatcttgag tggttcaaag gtctctcttg actatgatcg ggcagatggc gtcagcgatg
     2641 aaggagaggc tgtcgatacg ctacccggga atgtcacatt taaagtcagt ggctcaggaa
     2701 gtgaattttc ggtaaataca aagatcaata ttaccaacga caacaatcgc ggaattatcc
     2761 aaatgagagg ctcaggatca agagtcgctg tgtcgaatga tgggaaaatc acaatcaata
     2821 cagcgactac tggcggattt cgcctgcaag gagattctag tcaaatccat gtatcaagtg
     2881 gtggacaaat ctcggtcaaa atggctggag atggcgacca acctggaaac aatgggatgc
     2941 gttttgtagg gaaaggactt tctttgatcg tagaaggagc cggatcagtc atcgatattg
     3001 ataagcaaag tggtcgttct agcgcagttc gttttgagaa cggtacgcaa aaactaacag
     3061 taactaatgg aggctcattg aaggtaagaa atgcagggga tggcaaatct tatgtgaaca
     3121 gactcaatca aggaaaccaa gcgatccaat tttatgacgc ttctggtttt ctagaaagtc
     3181 cagggtcagc tgattttacg gttactggag aaaaatcaaa tatcgatata cgcgcagatt
     3241 ccggagctac cgtagataca actggagata ttgatttgaa ctttatggct ggcgaaaaaa
     3301 cttatttagt catggtcggg aaaactgggg aagataagac tggcatcatc agtggggatg
     3361 tgttgaatgt agacttagaa tctcctacct atttcgattt ccaaaacaaa cggatcggaa
     3421 gcgagactaa tggcataggt ggctgggtat ttcaaggaaa tggcgattct tctttgaaga
     3481 tcgctcattc agaaattgct ttatggcgaa aggcaggagt aacgttcgat aactttgaaa
     3541 aagatccgga ttatttttct gatatgacag acttatcgat caatggaacc gacttaggta
     3601 attttgtatc aagccctagt gcaggtttaa caaatgaatt tgctcagaca ggtgccggaa
     3661 tgaaaaatta taatcgaatc agtgccaata accagcttcc gatgattgaa tccttaagag
     3721 taccaacgaa tgcagataaa aaaatctatg ggcatgtcgt tgttcccgaa ggtgtcgagg
     3781 caaagccaag agatgcttgg acaggcgaag tagaagtgac tttacgtatt acttatgcag
     3841 atacgacgaa atcacctgta gaaattactg ctctcacaca aggacaaaca gatgaaaatg
     3901 aagggacagg atataatccg tggggagaag gcgaacaagg cggtttgttt gaggttcaag
     3961 tacccaacga agaaagacta acaacggggg atcaagtgca aatagttgct gctaaaaaag
     4021 taagaggcgg ccaagaagtg acagaactgg actctgccaa aacgacagtc gatgtgattc
     4081 ctcctgagcc tgctaaatta agtaccacat tgatcaatgc agcttctcgc tcaatcacag
     4141 gtacaggcga agcaggagct ttggtttcat tgaaaaaaaa tcaagctgtt ttgtccgaaa
     4201 caactattga tgatacaggt aaatttgttc tttctatccc
//
LOCUS       DAZULG010000164         4222 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000164 DAZULG010000000
VERSION     DAZULG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.168"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..466
                     /locus_tag="ACG9CY_002679"
     CDS             14..466
                     /locus_tag="ACG9CY_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939130.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            480..1169
                     /locus_tag="ACG9CY_002680"
     CDS             480..1169
                     /locus_tag="ACG9CY_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939131.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1294..3645
                     /locus_tag="ACG9CY_002681"
     CDS             1294..3645
                     /locus_tag="ACG9CY_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS4939132.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3725..4120
                     /locus_tag="ACG9CY_002682"
     CDS             3725..4120
                     /locus_tag="ACG9CY_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939133.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1487 a          532 c          857 g         1346 t
ORIGIN      
        1 cgaggtgaga atcgtgaaaa aaacgatact aagtattttt tcagtattta caatattaat
       61 tgggggctat tttcttttga atttagattc tgttaattca aacgctcaaa taaaagatca
      121 aagagtagct gaacataata ataaaaaaat gagtttgacc aaaaatatct attctatgtc
      181 agatgaagaa ttgtataaag aatttggtga tttatccggg gaacagttag aagaaagtat
      241 tccaggaatt agtgactcag ataaaattgt tgtcttgtct gctggtgggc aattaatggg
      301 agatggcaaa tccgaattag ttagttataa acaaaatcac agacagttta ttgatgttaa
      361 tactgattcc aacgctattt cgattaagga tctaaaagag cttttaaata gtaaaagaaa
      421 tgaattaaag cagataaaag aaactaattc agtggaatta gagtaaagga agattcatta
      481 tgaaaaagat ttttggtgtt tctgttgggc tacttatgat gttaacattt ggaaatgcag
      541 tatatgctgc tgagaaagaa gttatttcag atccagagca gtacgcctca cgatactttg
      601 ctgattttga agaagaagat aaagtacttg cattgccaga aggtggtttt ttaaaagggg
      661 aagctaaaat tgtatctatg gataatccag atataattca aggttcatat aattcagaaa
      721 ctgatcctgc ttcagtttca gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa
      781 atgatattga aactactcca attatgcctc gaggagcatt tccacccgat gttggtacta
      841 gcggacacct tcttgtacta gcaggaggag gaagctataa atcacagcca tttagtggtg
      901 cgggatggcg ttttggaggt atgggtgtaa aaccagctga tggtacaggc aactttttac
      961 gttggaaatc ctacattgac gatggaagag taggaaatgt aaatcaagcc aacgcaacta
     1021 aatctggttc tattcaagga atggcattgt atgctcgtga aggattcaaa tactttgcac
     1081 caaacgggcc tggttggaca atgaatatag gtatttatta cacttataat cctgttgcag
     1141 gtacgtatta tgttgttcaa aatatttaat aaaacagcac aaagagattt ctttgtgctg
     1201 ttttattatt tttagttgtt gacaaacact aaaatataaa atataatcaa cacataaacg
     1261 aacgtatgtt cggcaagaaa gcaggggatt tttatggatg agatggtttt aagtactcag
     1321 aaatggctaa acaagaaata cagtaatgtt acagggtttg ataaggttcc agaaaatggg
     1381 cgaacagggt ggcctacaat ctatggactt attgaaggac tacaagtaga acttggtatt
     1441 actaatctag tagctaattt tggacctacc actgaaaaaa tgtacgataa tcaggttact
     1501 cctaaatggg gcaaaaatct acctaaaaat attgtatttc ttattcaagg tgccttttgg
     1561 tgtaagggga ttaatccagg tggcttcgat ggggtatata ctccatattt agatactgct
     1621 gtaaaagagt tgcagactga tgcagggttt aatggaagcg ctgtaactgg agttttggat
     1681 gcgaaatggg ccaaagcgtt atttgatatg agtgcttttg ttcttgttcc cggcggagat
     1741 agtaaaatta gaaaaatgca gcaatggtta aatgtaaatt atttagaata cacgggaata
     1801 atgccttgtg atggtattta tcaaagaaat tcaaatcaag cattaatttt tgcattacaa
     1861 gctgaactag gatattctcc cagtgaagca acagggtctt atggtaatgg gacaactagt
     1921 aaaacttatc ctgtgtctga aggtaactcg ggaaactatg taagaataat tcaatatgga
     1981 ttatatgtta atggttattt tgaagacggt acattcgatg gtattttcac taaatatatg
     2041 ggtttagaag tactggcatt ccgaaaattt atggtattgc ctgaatatac tgaaatagca
     2101 gatgttgttg taataaaagg actattgtct agcgctggag atattagaag atctgcagat
     2161 ggcgcagata cttctacgca attaactaga tctcaaatcc aaacattggt tgataatgat
     2221 ataaaaatag ttggtagata tcttactggt actgttggaa ttggaaaaga tgaacgaaat
     2281 aagtatctaa ctacagagga attgaataat attttttcta aaaatttatc tgtgtttcct
     2341 atctatcaag atggaggtgc agcattagct tattttactt atgatcgtgg cttatcagat
     2401 ggtaagaagg ctatagatgc agcaaaaaat ctaaatatac cattaacaac agttatttat
     2461 tttgctgttg atctagacat gttaggggaa gaaattcttg catatgctgg agaatatttt
     2521 aagggggtat ctgctgtaat gtcatatagc gggtatcaaa cgggtgttta cggaacaaga
     2581 aatgtttgtt ctcaaataat caataacggg tatgcttcgt ttgcatttgt ttcagatatg
     2641 tcgactggtt atagtgggaa tttaggtttt cctatgccta gacaatggtc ttttgatcaa
     2701 tttgttgaat ttaccatagg aagtggcaac ggagctgttg gtatcgattt aatcgctaca
     2761 tcgggtagag atagcggatt taatgagtta tcaaatgata ctaataatga taattacatt
     2821 gccaaataca atcagaaggt tataaatcag atggtacgtg cgtatcaata tctatcacaa
     2881 gctggtcttg ataatccttg gaaccctcat ttgacatttt atagatatgt aaattattct
     2941 ggtttgtcat gggacatcat ttcttctcct gtaacagagc atgatagaaa aatttatgat
     3001 gaatatagga ataaattaac taatagcgag ggcctgtata attattttat agatcctaat
     3061 actggttcaa taataggttt gcctcactta attgtgacgt tacaatcaca gatgtttcta
     3121 gccgacacaa tcaatgatag tgtttctgac tttgcaggtt ggttaggaga tttaatgact
     3181 tgctggggtg aagttaagaa attaggaata caaatggagc aaggtgtatt tgcattagta
     3241 ggtacggctg caggtggaac atttagtcta gaagatttat accaagatgt tgacgcggta
     3301 aatgtttata ccttagcaaa aagaaattct gattcaacag ctttatcggc tttaaaaacg
     3361 tactattgtg ataagggtgc tattgataga tttaatcaat ttgttaagaa cagatttggt
     3421 tcttcagata aaatcgcttc agtaacaaat agtttattag aaaatgacga aacagttgat
     3481 atcttaggtg tagggataat ttcatttttc attgataaaa tagttaataa ttcacttggg
     3541 acagatttta cttatcttga ttttattgga gatgagggga aagttgcaaa tggattttct
     3601 gaaaaaatta ttcaatttca agaaaataga agtaaactta attaagaatc gtttactatt
     3661 taaacagtgt ccttttcctt tttaaatata tacgataaaa taagtatatg aattgtaaaa
     3721 ggacatgatg attatggaag aaatagaatg gaataatctg actcaaaata ataaaaataa
     3781 ttctggatgt ggaattatct ttatgtttgt tatagttttg ggaatagtag tacttttaat
     3841 aactgtcaag atagtagatt actttgcgaa tactcctgaa gaacaaatac agagaaaact
     3901 tcctgagttt gaaagagtac caccttatga tccaagagat aattcatttc aagtaaaaaa
     3961 attaaaagag tctccaaatg aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa
     4021 agaaaaaaag aaagaataca attatattgt aaaggcaaaa gtatcaaaaa gatttatttt
     4081 taaccctaaa ttagaaatat atagtgttgt aatagaataa tagtgttaaa aaaattgtaa
     4141 aagttaaaga agtcaaattc tgatgtgaaa aataaaatat accatggaag acataaaaga
     4201 aaaactggat aactagttta tt
//
LOCUS       DAZULG010000165         4115 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000165 DAZULG010000000
VERSION     DAZULG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.170"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            294..665
                     /locus_tag="ACG9CY_002683"
     CDS             294..665
                     /locus_tag="ACG9CY_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4939134.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(815..887)
                     /locus_tag="ACG9CY_002684"
     tRNA            complement(815..887)
                     /locus_tag="ACG9CY_002684"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(852..854),aa:Gln,seq:ctg)
     gene            complement(933..1361)
                     /locus_tag="ACG9CY_002685"
     CDS             complement(933..1361)
                     /locus_tag="ACG9CY_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939135.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(1421..2179)
                     /locus_tag="ACG9CY_002686"
     CDS             complement(1421..2179)
                     /locus_tag="ACG9CY_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4939136.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2474..2671
                     /locus_tag="ACG9CY_002687"
     CDS             2474..2671
                     /locus_tag="ACG9CY_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939137.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2777..3259
                     /locus_tag="ACG9CY_002688"
     CDS             2777..3259
                     /locus_tag="ACG9CY_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939138.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3317..3913)
                     /locus_tag="ACG9CY_002689"
     CDS             complement(3317..3913)
                     /locus_tag="ACG9CY_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4939139.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         1288 a          664 c          791 g         1372 t
ORIGIN      
        1 cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat catttccttg actttttgaa
       61 ataattcttc ttgattcatt ttgaacacat cctttgctat caacatgatt ttttacatgg
      121 acaaaacgag aaaaactgat ttatccacac gtcaataata tcaaataacg atcattttta
      181 ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa aatctccaac ttgggacgga
      241 tggaatttct ttaactgacc catatactga atatataaca aggaggtaga aagatgaaag
      301 tgattaaata tttgattgga ctggcggcga ttagtggtat tttgctattt ggactgaaat
      361 attatactga aggaagttat ggagaaatcc ctggtatact tgatcagcta aatccattag
      421 ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga ggaagtaaat gaatatggta
      481 cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa agaacgaacg gttgaattta
      541 atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat tagcaataaa ggagcacaca
      601 tagaaacgta tgaggaagtt cctagagaaa aagttccgga gaaagcatta gcaattattg
      661 ggtaaatcag tttttgtatt aagaatacgc ccgattttat tattcataat ttaattatgt
      721 aaactaaata caagtagtaa aaataataca ataatccccc tgcaaaaaga ttgttctttt
      781 acagggggtt cattttttca gcaagcacat caaattggga tggcagggtt cgaacctaca
      841 attgcttgat tcagagtcaa gtgccttacc agtttggcta catcccattt attcaacaaa
      901 ataatcatag cactttccta aaaaaacgca agttattttt cttgatcgga tagttgttgc
      961 tttactcgta aaaggcggcg tactagtttc ttttctaatc cagcaggaag ggcaatcagt
     1021 tctgctactg acaagacggt gtccaataac tgatattctt tgacaggttc attcataata
     1081 tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag tatactctgt tcgagcaatt
     1141 agaaatttca ataaatcggt ggctttctcg tactcttgat ctgcgtaata aacaagagat
     1201 tgacgataca atttgatttc cagttcattg agggtagcag gtctgcgacg ctcgttccac
     1261 tcattaatag agactttacc ttgaacattt cccttgcagt cacggacaaa aataatcgga
     1321 atttcatttg gatccatttt tttatgtgtg aatttttcca ttatctacgc tcccttcaaa
     1381 ggtttggtat cttgagtata agggattttt gtttgaatta ctagcttttc tccaaagtta
     1441 gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc gttgatcggt actgctcgat
     1501 tttttacacc atagggatcg ttgacataaa tattttcttt gtcatatcca gtgactacac
     1561 aagcatgaac aagaggagtg actcttactt ctccattatt tgtggtccaa gtcataaaat
     1621 cagtttcatc tgggacttca aaattgatag tcacagaagt ccagacagga tttcctttag
     1681 atagtgcttt tttgatatca tcgagttgag catcggtttt tggaaaagtg tgataatccg
     1741 tttggacgat ttgctgagca agggaggcaa ctgggggaac atagacgacc atagcccagt
     1801 ctccgccact gatatcacca acaaatcctt cattaggatc gccatgagtc ccatctgcat
     1861 tgaaaacagg gacgtaatcc aattgctcag ctaactggtt tttagttgtt tcataaccat
     1921 aaaaagataa aagcatagaa agcgaagtga cttcacagcc attttctaaa gaaggagaat
     1981 caaactggct ctctaatgga acatctagta aaacttctgc tttctctttt gtctttttcg
     2041 ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg ttctggagaa tggatatgta
     2101 ggtaataact tgtaccgctt aataaaagag cagctgtgcc ccctagaaac aatagttttt
     2161 tgaacgtttt tttcttcatt tgaaatctcc cttttcagct gtttatcttt cctattataa
     2221 atgtattttt attggaaatg gaagtattat attttaatat gtaggaaata attagttttt
     2281 aaggaatatg ttttaatttt ttgaaaaaaa ttcttctaaa tacaacagct gttttaattg
     2341 tgaaaacgaa aacagtacgc tataatgcct gtaaggttga tttttaactg taaataaaaa
     2401 tcaatgagtt aaatctccaa agaaatagca gtggtaatgt ggagaaaagt tccagcagtt
     2461 taggaggatt ttgatgacgg aagaaatgat taatttagga gaacaatatg catgcaaacc
     2521 aattggtttt acaaaaacgg tcattggtga agttgtatca aaaatgacaa actgcgccgt
     2581 ggtcaaagtt gcccaatgtg ctgcagagga ccaagaatta ttagatgaaa aagcaagcat
     2641 ggttgttgca aaatatgata catttgaata aagaaaacaa ttggacgtgc ggcgtgctgc
     2701 aagtccaatt tctttgagaa ggtaaatcag tattcttttc ctattttctg gagaagaaaa
     2761 agcgaggcga gttgtcatgg taacattctt tacggttcta ttgtttttat tcgttgttgt
     2821 gatgttttta tttctttggc tggttcgaaa agaaattatt tatcggactg tgcgaaatcg
     2881 atgggtctac ttggtgatac ccttcttagt gtcactagtc atttggtata cactcatcag
     2941 tcatcctaca gcagatgaat tggcaaaagg gatcttgtca gcaatgattt ttattagttt
     3001 tttgttggac agtagaggaa tcacagaaga aggacttgtt ttgaatagct ttgacaaaaa
     3061 aggtgttcct ttttctgaga tcaataaaat tgtattatat cagccaaaag gttctaaaat
     3121 cgtaaaaatg aattttttcc gaaacggatg gcgaggaccg atgctgaaat tttctgcatc
     3181 attagaagaa ttagttccat ttttgtccca acgcttaaac gatgaggcag agattgatat
     3241 tatgattgat ccagaataaa aacagaggtt gcgacaagat gtttgtcaca acctctgttt
     3301 tttatttcaa ataaaattat ccaaatacgc ggaagcgatc ggcatcgttc atgtattctt
     3361 tttcctcagc cggtgtttct ggtgaaccga atactaattg cgaacgtagt ttccagtttg
     3421 aagggatatt ccattctttt gcaactgctt cgtcaataac tgggttgtaa tgttgcaagt
     3481 ttgcgcccaa tcctttatca actaatgcta cccaagtatt agctgttgcg attccgtttg
     3541 attgttctga ccaatctggg aagttgtctg catataattc aaattgttct tgtagacctt
     3601 ttaccacgtc agtatctttg aagaataata ctgtaccata accatttttg aaaccagcta
     3661 atttgttttg tgtatttggg aaagcttctg ctggagtcaa tgggcgaagt gcttcttcag
     3721 tgatttccca aagtttttca tgtgcatctc cgaaaaggat aactgcgcgt gttgattgag
     3781 cattgaaagc agttgggctt tctttaattg cttctttgat caaagttgtt aattcttcat
     3841 tagaaagtga aacattacgt cctagatgat agattgaacg gcggtttttc aaagtgtcag
     3901 taaaagttgt cataataaaa tttctccttt gatttgcgtt ttgcattttt ttaattgatt
     3961 acgaaatgaa gtatagcacc ttacaaaaag taagtaaaca gatatgctca caaaaaataa
     4021 gcgaagttca aaaaacaatt ttaataagaa aaatcgttat gtaaggattt tcttagttat
     4081 cgctttcatt gttgtgtttt aggaattttt gctta
//
LOCUS       DAZULG010000166         4033 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000166 DAZULG010000000
VERSION     DAZULG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.171"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1648)
                     /locus_tag="ACG9CY_002690"
     CDS             complement(143..1648)
                     /locus_tag="ACG9CY_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4939140.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1645..2331)
                     /locus_tag="ACG9CY_002691"
     CDS             complement(1645..2331)
                     /locus_tag="ACG9CY_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4939141.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2524..3945)
                     /gene="gndA"
                     /locus_tag="ACG9CY_002692"
     CDS             complement(2524..3945)
                     /gene="gndA"
                     /locus_tag="ACG9CY_002692"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS4939142.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1110 a          854 c          690 g         1379 t
ORIGIN      
        1 atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt tttttataat
       61 gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt ggatatataa
      121 aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga ataaagattc
      181 taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta tagttttcaa
      241 ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta gctcttgctt
      301 tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt attccttcgc
      361 caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag atatggactt
      421 cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt aattgttcaa
      481 agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct aaagtgatca
      541 catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact tcttttcctt
      601 tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag tctagcatct
      661 cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat gattcttcta
      721 atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct tcaataattg
      781 caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt tgttcaatat
      841 acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc aattcatcat
      901 ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata gtatccattg
      961 tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa cctaaaatac
     1021 tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc ttatttcgaa
     1081 tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt tctcttgtac
     1141 ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt gtaacgatcg
     1201 ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca
     1261 agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc tcagctataa
     1321 aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg acctcatctc
     1381 cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt tcttcatccg
     1441 agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt ctttcttttg
     1501 ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta aacacaacga
     1561 atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct ttcagttctc
     1621 ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc cgcgtacagt
     1681 ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac gaatatatac
     1741 gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta gtaaaacgtc
     1801 acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt attctctttt
     1861 tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa
     1921 atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa tatcgattcg
     1981 acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt tgacgatgta
     2041 gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc ttgcagtcat
     2101 cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta aaccgttcaa
     2161 ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg ccgcatctaa
     2221 accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca actctagctc
     2281 gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca tttattattt
     2341 tatcctttct attctactgg ctttttaaaa actctatcat tataccgata atatgcttaa
     2401 aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag tctactcata
     2461 acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac agccgaaaga
     2521 ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga cacgctcata
     2581 ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca gacgttctgc
     2641 tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta cgccagcttg
     2701 gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga tttcaacgaa
     2761 gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat ccgtaatttt
     2821 ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga tttcaccaaa
     2881 tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc cttgggcata
     2941 actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt cttttttgtc
     3001 tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc
     3061 tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa gtggcacacc
     3121 taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag ctgcatcaag
     3181 gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc
     3241 agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa agatatcagc
     3301 catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt cagcaatcag
     3361 ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc cggctccatt
     3421 tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa tttgctctaa
     3481 gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg aaggtccttt
     3541 taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc cagagttggc
     3601 taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt caatcaaaat
     3661 atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg ccggtcctgc
     3721 tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt cttcgattgt
     3781 atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg ttttagcccc
     3841 tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg ctagattttt
     3901 acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa ctcctcctac
     3961 atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat tgcaaactaa
     4021 agaacaacat att
//
LOCUS       DAZULG010000167         4012 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000167 DAZULG010000000
VERSION     DAZULG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.172"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACG9CY_002693"
     CDS             85..1434
                     /locus_tag="ACG9CY_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4939143.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACG9CY_002694"
     CDS             complement(1562..2152)
                     /locus_tag="ACG9CY_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4939144.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG9CY_002695"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG9CY_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4939145.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1148 a          741 c          784 g         1339 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tc
//
LOCUS       DAZULG010000168         4000 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000168 DAZULG010000000
VERSION     DAZULG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.173"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2907)
                     /gene="brxC"
                     /locus_tag="ACG9CY_002696"
     CDS             complement(<1..2907)
                     /gene="brxC"
                     /locus_tag="ACG9CY_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFS4939146.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRD"
     gene            complement(2932..3507)
                     /locus_tag="ACG9CY_002697"
     CDS             complement(2932..3507)
                     /locus_tag="ACG9CY_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFS4939147.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(3507..>4000)
                     /locus_tag="ACG9CY_002698"
     CDS             complement(3507..>4000)
                     /locus_tag="ACG9CY_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFS4939148.1"
                     /translation="EKNEHENIFFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNI
                     DNQKLVNLISILMIDDLFNEFMTEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISK
                     WKDYTIKRLIAAYQTNLIETGLVRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALG
                     GIL"
BASE COUNT         1191 a          764 c          549 g         1496 t
ORIGIN      
        1 atcgcgatat tttttgattg tataaaaaaa gtcttcaaca tctgacgttt gaacaattac
       61 ttcaagatat ttttttattt gctcatattc ataagcacta gggtaggtat aagtgttttg
      121 taaatcaaaa taagagcgta aattgtctaa ttgacctttt aaatatttct tgaaattttc
      181 cattgtatgt tcattgttat tttcatcaac aatattacga ctttgaaaca gttctctacc
      241 gatttcgtca accaattttt ttgcttttgg cgaaatcacc gcgcttggtt caatttttat
      301 cttttcactt tctcgtcgtt tttgaataat cattacaata ctatttggat catccatccg
      361 tgaaatttgt acattgttat aaagtaaatc aattttacca gacatgaaaa gtttagcgat
      421 gcaccattcc acatcaagtt ctgtgaatcc atacggttgt tttgtttcaa aacgatcacg
      481 aattgttttc atcgtaatcg ttaggtgatt tctcttttga tcattgataa aattgaaaat
      541 ttcctgaact gcttgctcat ttgatggatc ttcaataatt tcatttttgc tttctttcac
      601 taaatataag atatcttctt tttctttagg cgcatcaatt tggtttaatt tatggtatac
      661 ttcttcagcc attttctgaa gtgctgattg gaattttgct ttaaagtcgg agcctgtcag
      721 ttgaatttct gcaccgttat agaaaaattt tgcagatttc aacagttcat ttaattgatc
      781 gttgatactt gcacttagtt cctgtaattc accagatttt tccgaacgaa tcgcgtcaaa
      841 gttctttact acactcgaat aagtcgttgt taaataaaga ttcattctag catacattgc
      901 tatatcatct ttaaattctt tatctgatgg caattctacg aataataagt tacgggaact
      961 cttaagcgca agttctgcct cttgacctgc taaatcagag tatggtgaaa taaaattaac
     1021 tgtcatttca gcatttccag cttgcttgta tggacgttga tctaacactt gtgcataagt
     1081 aaacgtatac cggtttttaa atttatttcc acctgacatg cctaattttt cataggcaaa
     1141 actatttcgg tctagaacgc cgtcaaaaat tcgtttagat agttctacga tcaaatcact
     1201 atctgtaata ttttgatttt taatctcacg agtaatttcc tgttcttcat cagtttggaa
     1261 aatataacta tctaagttct tccgcacaaa gttttcctta attaaaaggt ccaatgcttc
     1321 ttctactcgt tttttagttt ccaggcggtc ttcgttgata tttcttatca tcaagctcgt
     1381 aatattttca acatttggtt tgatggcttg atcaacatat ttaatcatga ataaaattcg
     1441 taagacattt acgttaaagt tatctatatc agttggtctt tgaatatgag aattatttgt
     1501 agcttgtgta atcactcggc taattgctgt atcaatccaa cgttctaacg agtaataaaa
     1561 ctgatctaaa gaaaccaaac tggttgtatc attttcctta tttcgttcag cggcttcttt
     1621 aaacattgct agtaatgaac gttcaccaga cgataagttt gatccttgat agctgtgatt
     1681 acgaatctcg gttagaattt gccccaataa attgaattga tagggaataa atggataaac
     1741 ttctgcgtaa ttttcagcat cttggaagag ctttaattca ggactatcaa caaagagtaa
     1801 tttatttttc aattctgttt gaacagtttc aaattgtaag gctaaagaat cattggcaaa
     1861 ctcttttttc ttcaaaatac gttctttaat tacttcatcg gcatttgctg aagataaact
     1921 cacagttgtg gcaaaacgat ccttgatctt agaaaaatca ttatcagaat tgttatcaaa
     1981 gtttgttacg tgatcaatcg cttcctgact tgtcactaat acccaagcct taccttcaca
     2041 aattcgaccc aaatcttcca caacggtttg taactgcaaa gtcaactcac gattaccgcc
     2101 gatatattgt ccgacctcat cgactagaaa ggcaatatga tggtctgctt ctttggaatc
     2161 aagatagctt ttaactagat ttgcaaagtc atcaatgctt attaaataat ttcctgatgc
     2221 tttttctaaa aaattatcaa tagcggcttc actcataaaa tcgattttaa gtgccgcttc
     2281 tttgatatca tcttggatcc aatcaaactc atggcgctct tcttgccatt ttgccccatt
     2341 aatctcttca aaagcagctt tgaatgcatc aaacttttct tctttaatta accgacgttc
     2401 aaaatctgct agatgtggaa tcgtgcttaa tccttgcatt tcgttaaata cctttaaaaa
     2461 aacattcacg attccgtttt tgtctttttt accacctgca tcactttttg aatcaatatt
     2521 aaacagaata acatctgtat ttatttgtga gcaccgttcc atcgttgcca aagtcagtgg
     2581 atctttgatt ttcccatctt ctttgaagta atcaatggct ttcttgtcat ttatttctcg
     2641 atttgcgagc aagtaagaaa taatttttaa gaaatgagat ttacctgaac caaagaaccc
     2701 ctgaatccaa acacccattt tatctgttgg tgtattgata ctccgttggt aggcatcaaa
     2761 aattgtttta aagtgttttt gtagttcttt cgtaaccacg tattcttcta attcttgttg
     2821 aacattttca tcctttgctt gaccgacctt aataactcct tgaatatttc ggtcaattgg
     2881 ttttgcaaaa atatctttaa ttagcattta atctcctacc ttccatccca actaaattct
     2941 aaatgctcga taataattat catctttatt ggtattaaga attgtcaaac ttaattcatc
     3001 atattcccct ggatacatca tcacaactgg tacgcgatca aatacctgat gcattttatt
     3061 taaaatagag tgcgctctaa ttaatggata taattcgcca attcctgtca ggaacacaac
     3121 tgatttttct ggtgtatggg catccacata ttcaaaaaat acattttctt cctcaggtaa
     3181 atccattttc aataaacgac caatgctttc cactaatttt tgtaaaccac gtcgttcttc
     3241 catcttaatc gctttttcag tgaaaccgcg tttttctaga tactctaaca taatcacata
     3301 gagattataa actttcactt cataattatc accaacagat gttccagata tatcattaat
     3361 ataatctctt acaattaact cgtctttcgg atcgtagtca aagatataat agcctacttc
     3421 attacctaaa ccacgattca ataaaaaatc ttctttttga attacttcat tcaacttttc
     3481 caaatgctct tgaatcgata atgacattat aaaattcctc ccagtgctag tacaatatct
     3541 ttacgattct tgtcaactaa ccattgaatc aatcgtttat ctaataatat cggagtcatt
     3601 cgtccatatt ctttttcccc tctgactaat cctgtttcaa ttagattcgt ttgataagcg
     3661 gcaataagcc gtttaatcgt ataatctttc cattttgaaa tatcttctga acttctccgc
     3721 ttctctgtaa aaaaagaacg gtaatcaact ggtgttaatt gcaaatttcc tttacgtaga
     3781 ttttcagcat atacctcagt cataaattca ttaaaaagat catcaatcat aaggatggaa
     3841 atcaaattca ctaacttttg attatctata tttaactgat tgaaaagttg caatccagct
     3901 tcatccagtt gactaactct ccttttcacg ttctgataga tttctttttg acggttactg
     3961 ctcactgcaa aaaaaatatt ttcatgttca tttttctctt
//
LOCUS       DAZULG010000169         3960 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000169 DAZULG010000000
VERSION     DAZULG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.174"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(90..1346)
                     /gene="tyrS"
                     /locus_tag="ACG9CY_002699"
     CDS             complement(90..1346)
                     /gene="tyrS"
                     /locus_tag="ACG9CY_002699"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS4939149.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(1458..1663)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1926..3227
                     /locus_tag="ACG9CY_002700"
     CDS             1926..3227
                     /locus_tag="ACG9CY_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4939150.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(3296..3895)
                     /locus_tag="ACG9CY_002701"
     CDS             complement(3296..3895)
                     /locus_tag="ACG9CY_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4939151.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         1180 a          791 c          684 g         1305 t
ORIGIN      
        1 cgacaaccag cgttctattt taggatgggt ttcaaaattg gaatgttgta atagtatttc
       61 cctttttcaa ggtaaaaact tactaaattc taatcaatta ctttggccaa aaagtaattt
      121 ttcttacctt ttcgaataac cacaaactta ccatcgaatt catcggatgg atttatgaca
      181 aaatttaaat cggtaacgcg gtcaccgtta atacttatag ctccgtttga aacatcttca
      241 cgtgcttgac gtttagatgg ttcaattttt gtggaaacaa gcagttccac gatattttca
      301 ggtgtgcttg aaatttcaac gttcggcatt ttaccgaatc cttgcgcgat ttcttccgca
      361 tttagttctt taatatttcc agagaacaat gcttgcgtta ttttttgcgc ttctttcaag
      421 tcttcttcac tatgcacaaa tcttgtaact tcttctgcca aacggcgttg tgcttcacgt
      481 ttctcaggtt ctgtctctac ttttttcgct aagtcttcga tttcttcttg agacaagaaa
      541 gtaaagaatt tcaagtattt cactacatcg cgatcatctt gattcaacca gaattggtag
      601 aactcaaatg gtgaagtttt cttcggatca agccaaacgg caccgcctgc agtttttccg
      661 aatttcgttc cgtcagcctt cagcatcaat gggatcgtta aaccgaacgc ttctgcttct
      721 ggtccttcta attttcggat taggtctaat ccagctgtga tatttcccca ttggtctgct
      781 ccaccgattt gcaattgcac atcgtatgtt ttatgcagat gcaaaaagtc gatagattgc
      841 aggatttggt aagtaaactc tgtaaatgag attcctactt ccaaacggct ggcaacgata
      901 tcttttgcta acatcgtgtt gatgttgaag tttttaccgt agtctctcaa aaattcaagt
      961 aaagagattt ttgacaacca gtcgtagttg ttcacaaatg tgatatttgc atctttacca
     1021 aacaattttc tcatttgatt ggaaagtgcg tccacgttat gttgcacttg ttccatcgtt
     1081 tgcaacacac gttctgtttt gcggccgctt gggtcgccga tcgttcctgt tccgccgcca
     1141 atcagaatat atggatgatg gcctgccaat tggaatcttt tcatcatcat aaatggaatc
     1201 aaatgtccga tatgcatcga atcaccagtt ggatcaacgc cgcaataaag tgaaatgctc
     1261 ttttcttcaa cgagctcgcg aagtctttct tcgtttgttt gctgattaat ggcgtcgcgc
     1321 caagttaatt catcaataat attcatttta ctgacctccg tgtagttttt ttatgaaagc
     1381 aggaacctgc tagcatgtgt aattaattat ttcttcagga taggatagga aataaaaaaa
     1441 gtcccttggc atataagcca agggacgaaa tctcgcggta ccacccaatt tgtatgtcta
     1501 gatatccaac atacctctcg aacatttaac gctgtcgcgt ggcaatcact gccaaagact
     1561 cctgaaatgt aattcgcttt aaagcatgta ctggtttgca gcgaccacca gctttctgaa
     1621 acattggctt taacaactac ttcgtttcga tcttcgtctt gttattatta atatgtgtct
     1681 atcatagagc gcaacttttt ctttgtcaac agaaaaagga tatttttttt aaattcctca
     1741 ttttttcttt tttgaatccg ctctaattcc gaacatttta ttttcaatta ttgttaaaag
     1801 aaataacttt ttctgtattt tttctgcaat ttatcgagaa gtcactttac tttgcctctt
     1861 attctttctt cgttacaata aggaatggga caagtaaaaa aatgaaaaag caagggacga
     1921 ggtatatgga aaaaaagcaa aaaattattt taggagccac ttctgcttta actatcgtta
     1981 ttttaattct aagtttgttt tttgtcatcc gatcatctac aaaaaaagaa ccaatcattc
     2041 ctgaaaaaaa aataacagaa aaagcaaaag aaccgacaga tgataagcaa caactagtta
     2101 ctcaagcaaa aaatctggct caaagctacg attatgacca agcgattgcg ttattggaaa
     2161 aagatagtaa gaacggatct actgaaacac aacagcttct tcaaacacta aaaaaggaga
     2221 aagaacaact gatcgactgg tctgatccta ctcagatctc tcatgtcttt ttccacagct
     2281 tgatcgtgga cccgcaaaaa gccttccact ctcaacaggc acaaggatac aaagattata
     2341 tggtgactgt tgaagaattc aaccgttcga tcgaacaact ttatcaaaat gattttgttt
     2401 tagttaattt gaatgatttg atccaaaaag acgaacaagg aaatctttcc tttaacgggc
     2461 ttgcgctacc tgaaggaaaa aaaccattgg ttctttcaca agatgatgtc agctattatg
     2521 aatatatgga taattccggt tttcctagcc gattggtcgt atcaaataac aatgaaatca
     2581 aaaatgtcta taaagagaaa gataaagaag aaatcggtga ctttgacatg gttcctctga
     2641 tcgatgcctt tgtcaagaaa catcctgatt tttcttatcg aggcgcaaaa ggtaccttgg
     2701 ccctgacagg ttacaacgga gttttaggtt atcgaacatc aaaatctcaa tatggagaca
     2761 atgaaaaaac aaaaaaagag attaaagaag ctaaaaaagt ggcggaacag ttgaagaaag
     2821 acggctggag ctttgcttcc catacatggg gtcacttgaa tatgactcaa gcttcactag
     2881 aagatgtaaa aaaagacaat gaattgtggc agcaagaagt tgcacctatc ctaggaaaaa
     2941 caaatattct gatttatcca tttggtgcag acatcagcga ctggcagcct tattcagcag
     3001 aaaatcaaaa atttgcttac ttgaagagtc aaggtttcga tattttctgt aatgtcgatg
     3061 cttctacacc agcttgggga caattaggta cggatttcta tcgaaatgct cggatcaata
     3121 tcgatggcat tcgttttcaa tctgatttag acggaaaaaa tcctattctt gatccattca
     3181 tcaatgtcca tgatgtctat gacaaagaag cacgcgaatc tgcttgattt tacgtcattt
     3241 atgaaccaaa aaagccgaac tgacatttta tcgtcagttt cggctttttt atgctttatt
     3301 tcttcttcgg tgcgattccc ttgatcagga cttctgtcca ttctgctgta tatggaagca
     3361 gttttgcgta tatctccggc ttagaaattc cttctaccat tgcttgaaaa tagaggatca
     3421 agaactcatc cgaatatttc aaatccactt ttccttcttt ccgccctcga tggaaaagat
     3481 caagcatgat actgaaactt tcttgcgtat acgcatccat cgcgtcagaa aattcttttt
     3541 tttctttttt tgtatagtat gtcatcaaat cgatataaaa ttgtttgttc actcgattga
     3601 gataacggat tttgttttca ctcatggcaa tcagtgtttc ttcaaatgga agattttccg
     3661 caatcaacgc tttcgcttct tgccctaaat cattcataaa tgcaaaaaat acttcataaa
     3721 tcagcccttc cttgctgcca aaatatttaa aaatcgtcgc tttacttacc tgagcgttct
     3781 cagctacctt atccattgtc actccagcgg ttcctatgtc tgtgttcatc aagtcaaaag
     3841 ctgcttgtaa aatctctttc tttttctctt gtgctctctt ttcaaaaccg ttcatttgta
     3901 gagcctcccg taattttaaa cttttataat atatatagtt caaatttatt ctgaggttta
//
LOCUS       DAZULG010000170         3931 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000170 DAZULG010000000
VERSION     DAZULG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.175"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG9CY_002702"
     CDS             <1..156
                     /locus_tag="ACG9CY_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS4939152.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="ACG9CY_002703"
     CDS             175..1452
                     /locus_tag="ACG9CY_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4939153.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="ACG9CY_002704"
     CDS             1462..2118
                     /locus_tag="ACG9CY_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4939154.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="ACG9CY_002705"
     CDS             2118..3494
                     /locus_tag="ACG9CY_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4939155.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3931
                     /locus_tag="ACG9CY_002706"
     CDS             3624..>3931
                     /locus_tag="ACG9CY_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4939156.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     "
BASE COUNT         1585 a          418 c          688 g         1240 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta a
//
LOCUS       DAZULG010000171         3874 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000171 DAZULG010000000
VERSION     DAZULG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.176"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1155)
                     /locus_tag="ACG9CY_002707"
     CDS             complement(1..1155)
                     /locus_tag="ACG9CY_002707"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS4939157.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1340..3781
                     /locus_tag="ACG9CY_002708"
     CDS             1340..3781
                     /locus_tag="ACG9CY_002708"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS4939158.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1234 a          725 c          847 g         1068 t
ORIGIN      
        1 ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga
       61 agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc
      121 gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata
      181 ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta
      241 gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt
      301 tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct
      361 ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct
      421 ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc
      481 ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat
      541 atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca
      601 gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg
      661 gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc
      721 caagctgtaa cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt
      781 gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc
      841 aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt
      901 tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt
      961 ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg
     1021 attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg
     1081 gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc
     1141 aatccctatt ttcatttcaa cacctcttta cttatgataa aaaacttact ttactaaggt
     1201 atcataaaaa gattaaaaga aatataaaaa agagaatatt taaggattgt taaaatttaa
     1261 tcttagaaaa aaataactgg aacaagagct atttgttttt acagcaaaga atggtatatt
     1321 tttgtaggag gtggcttact tgaaagaagt cgttatgata gatgctgcaa gaacgccgat
     1381 aggaaagtat cgcggaagtt taagtccatt tactgcagtt gaattaggca cacttgtgac
     1441 taaaggatta ttagataaaa caaaactgaa aaaagataaa atcgatcaag taatatttgg
     1501 aaatgtcctg caagctggta atggtcaaaa tgttgccaga cagattgctt tgaacagcgg
     1561 tctccctgta gatgtcccag caatgacgat caatgaagtt tgcggatctg gtatgaaagc
     1621 agtgatctta gcacgccagc tgatccaatt aggtgaagca gaacttgtga tagccggcgg
     1681 aacagagagt atgtctcaag cacctatgct gaaacagtat cagtcagaaa caaatgaata
     1741 tggtgaacca atttccagta tggtcaacga cggattgact gacgcatttt caaatgcaca
     1801 tatgggatta accgcagaga atgttgcaac acaattttct gtgagcagag aagagcagga
     1861 tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg gtgtattttc
     1921 tgaggagatc atcccagtca agatttctga tgaagacgtg ttatctgagg atgaagcagt
     1981 tcgtggaaat agtacattgg aaaaactggg cacgttacgt acagtattct cagaagaagg
     2041 aactgtaaca gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg tgatccttgc
     2101 atccaaagaa tacgcagaaa ataataatct gccttattta gcaaccatca aagaagtagc
     2161 ggaggtcggt attgacccaa gtatcatggg aatcgctcca atcaaagcca ttcaaaaatt
     2221 gacagataga tcaggaatga atttatcaac aatcgatctt tttgagatca atgaagcatt
     2281 tgcagcttct tctatcgtag ttagccaaga actgcagtta gatgaagaaa aggtcaatat
     2341 atatggagga gcaatcgcat taggccatcc aattggtgca agcggtgcac gtatcttgac
     2401 tacattagct tacggattgc ttcgtgaaca gaagagatac ggtattgctt cattatgtat
     2461 tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa cacataagga
     2521 tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa cggcgcagtc aattaataga
     2581 aaaaaatgta ttgacacaag aaactgcgtt gatttttcag gaacaaacac tttctgaaga
     2641 gctctccgat cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa tggggatcgc
     2701 gcagaatttt caaatcaatg gcaagaaaaa atggattccg atggcaacag aggaaccatc
     2761 tgtcattgca gctgcaagta atggtgcaaa aatctgtgga aatatctgcg cagaaacacc
     2821 tcagcggctg atgcgagggc aaatcgttct atcaggaaaa tcggagtatc aagcagtgat
     2881 caatgcagtt aatcatcgaa aagaagagct gattctttgt gcaaatgaaa gttatccttc
     2941 aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact cgggaattca tggggagttt
     3001 ccatgcatac ttatctattg attttttagt ggatgtaaaa gatgcaatgg gcgctaacat
     3061 gatcaactcg attcttgaga gtgtggcaaa caagttacgg gagtggttcc ctgaagaaga
     3121 gattctattc agtattttaa gtaattttgc tacagaatca ttggcttctg cctgttgtga
     3181 aatcccattc gaacgcttag ggagaaacaa agaaatcggt gaacaaattg ccaaaaaaat
     3241 ccaacaggca ggggaatatg cgaagcttga cccttaccgt gcagcaacgc acaacaaagg
     3301 gatcatgaat ggtatagaag ctgtagtggc agctacagga aatgatacac gtgcggttag
     3361 cgcttcgatc cacgcctacg cggcaagaaa tggattatac caaggattaa cagactggca
     3421 gatcaaaggg gataaactag ttggaaaact aacggtgcct ttggctgttg ccacagtagg
     3481 cggagcttcc aatatcctgc caaaagcaaa agcatcttta gctatgttag atattgattc
     3541 agccaaggaa ctagcacaag taattgctgc agtagggtta gctcaaaacc tggctgcttt
     3601 gcgtgcatta gttacagaag gaatccaaaa aggccacatg ggacttcaag ctcgttcatt
     3661 agcgatctca attggggcta ttggtgagga aatcgagcaa gttgcgaaaa aattacgtga
     3721 agcagaaaag atgaaccagc agacagctat acaaatattg gaaaaaatcc gggaaaaata
     3781 aaaaagcaat atctttagaa gaataaagca agatttacag ttattttatt tactgcagat
     3841 cttgcttttt tggctcttcg tcaaattgtg ttgg
//
LOCUS       DAZULG010000172         3785 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000172 DAZULG010000000
VERSION     DAZULG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.177"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..370
                     /locus_tag="ACG9CY_002709"
     CDS             62..370
                     /locus_tag="ACG9CY_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS4939159.1"
                     /translation="MFQNKGQRYVTKGVMDSLLVELQTLCWNLIDQNVQKKLPLDYLQ
                     IFEFSIEKGNQKLVHRQEEPEARKEYLISPKLRLESVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            397..696
                     /locus_tag="ACG9CY_002710"
     CDS             397..696
                     /locus_tag="ACG9CY_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFS4939160.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGTLPKNVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVCGLLVEGRLVWSQIGGTNE"
     gene            870..1415
                     /locus_tag="ACG9CY_002711"
     CDS             870..1415
                     /locus_tag="ACG9CY_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFS4939161.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEEKEKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFSRGNDEQSLE
                     ILTTIFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLSDHSLLFQ"
     gene            1571..1867
                     /locus_tag="ACG9CY_002712"
     CDS             1571..1867
                     /locus_tag="ACG9CY_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939162.1"
                     /translation="MKVDGECLTSSYKSIYEFFEEKGLDTDLLYQFYIAFRGQQLSFP
                     MKMYDRELVAQKIETMIQENHSIDSRKLTDRYGFSTRWIQSIIRQQQKEQHVHG"
     gene            1860..2750
                     /locus_tag="ACG9CY_002713"
     CDS             1860..2750
                     /locus_tag="ACG9CY_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HFS4939163.1"
                     /translation="MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGNVSDIEGLI
                     EKGPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYS
                     LRLAQEISYIYGFKDLMGETGEFSEEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQ
                     ALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLS
                     MKPMATKLQTELGKNVAYTEKNLQQDLDILEGEFEEVSSDIVSSDTVLSQLERLSHLL
                     ETNMITEEEFQQLKQELLKK"
     gene            2832..>3785
                     /locus_tag="ACG9CY_002714"
     CDS             2832..>3785
                     /locus_tag="ACG9CY_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4939164.1"
                     /translation="MKAKRLFLLGTILLLGGCSTVSQNEQKKQVDSTTYETETTRKES
                     STKEIETTTQETKTTESSSKATSSDTSDFQIKQELWDTNKASQLATFVPQWGKTLGQE
                     YKSYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGNAGFNLVAVYSDV
                     ETGEYLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFTDIINGQT
                     PQTPVVEESTQQATTTTTQSTSSSGISNWEAAKETVLNNKALWIYESPDKNEANTNII
                     ESTQYRKDLMQDENGEFYPVDISTDDIGNTVMNGGKQFRVYT"
BASE COUNT         1397 a          557 c          752 g         1079 t
ORIGIN      
        1 tgaagtccaa tcttctttat agaagattgg gctatttttt acgacaaagg agggaaaaga
       61 aatgtttcaa aataaaggac aacgttatgt cacaaaggga gtaatggaca gtttactggt
      121 ggaattacaa actttatgtt ggaatttaat cgatcaaaac gttcaaaaga agcttccttt
      181 agactatctt caaatctttg aattttcgat agaaaaagga aatcaaaagt tggttcatcg
      241 tcaagaagaa ccggaagcaa gaaaagagta tctgatttca cctaagcttc gtttggaatc
      301 agtgtctcaa aaaatatggg tcattgattc gggtgagtat caaacgatgt tattaccaga
      361 agaatactga tatctaaaag aaaggagcga aatgacttgt tagaaacggc tatcgaagta
      421 ctggaaaagt gtgcacagct agtcactact cctgaagagt ggggctatga atccgttacg
      481 atggaaaaag aggaaattga aatgggtacg cttcctaaaa atgttcatct accacgttta
      541 gtgatgacac atctttatat ttattgcgca ccagaagatg gaaaagacta tgtagtttat
      601 ttcattacag atattaccag ccagcgagaa tttgtttgtg ggttactggt agaaggacga
      661 ttggtttgga gtcagattgg aggaacgaat gaataagatc gattatttga gaatgattgc
      721 gaaatgttta gaagatatcc ttaccgcgat ttatcgaatt ctgaagaaaa aaggagaaag
      781 aatatgaaat attttcaact agtattgaca cttataacgg caatggtaat tgaattgctt
      841 gaacaaaaat tagcatagga gaaaaagaga tgattgaaaa agaattagtg accacacaaa
      901 cagaagtttg gcgagatcct catcataccc atcgctatgt tttcaaacga caatgggcaa
      961 tcaaaggaaa agaagagaaa gaaaaattag cagtagtcat taccattcgt ccaacagata
     1021 cagaaccatt tacgaatgat ttgagtatgt tattaatcga aaagaatatc cgacaattgg
     1081 gatttactgg ttttattgcc gtgaatcttt tttcgtatac aaaagcgaaa agtcctcggg
     1141 tattttctcg tggcaatgat gaacaatctt tggaaatatt aacgaccatt tttacagaaa
     1201 agaaaatcaa cactattatt tttgcttgtg gttcgattac ggcaacgagt caagtggctt
     1261 atgaacaagc aaagaccatt tacgatcaat tgagtgcgaa acaaaagaag atgaccaaag
     1321 tattgataaa tgcagaaggc aagttatctc acccattaag tattcatgtt cgtaatgaat
     1381 ggcaattgtc ggatcattct ttgttatttc aataaacgaa aataagtatt caatgtatat
     1441 gtttcttttt ctgttatttt acttgttctt ttgcagacta aacaacttgt aaaacctctt
     1501 gttatatgca ttatcaatga aggaatcaat atgatatttt taaaatgtca ggggaaatgg
     1561 aggtgagcaa gtgaaagtag atggggaatg tttgacgagt tcgtataaaa gtatctatga
     1621 attttttgag gaaaaaggac tagacacgga tttactttat cagttttata tcgcttttcg
     1681 tgggcaacag ttgtcgtttc caatgaaaat gtatgaccga gaattagtcg ctcaaaaaat
     1741 tgaaacaatg atacaagaaa accattccat tgattctagg aaattaacgg atcggtatgg
     1801 tttttcaacg agatggattc aaagtattat cagacaacaa caaaaggagc aacatgtaca
     1861 tggctaatga agaaacagta ggtttgagaa tcatagaaga agcattcaaa ttaccaatgg
     1921 tcaaagttga tcgtcgagca tttttgattc aaacatttca agggaatgtt tctgatattg
     1981 aaggactcat agaaaagggt ccacaagcat ttttatccaa agaaatgcta gatcgtgaag
     2041 cacaaaaatg tattcaatcg attaccttga aatcaagtgc ggtatcgtta gcaactggac
     2101 tgccaggagg tatcgctatg gcagcaacta ttccagcgga tctccttcag ttctttggtt
     2161 attcattacg attagcacag gaaatttcgt atatttatgg atttaaggat ttaatgggag
     2221 agactggaga gttcagtgaa gaagcaaaga atacgctgat attgtatttg ggaattatgc
     2281 ttggagtaac ttctgcaggt tctgccgtac gagcgatgtc tggaaagctg tctacacaag
     2341 cattgaaaaa gattcctcaa aaagcattga cacaaacgct gtattatcca gtaatcaaac
     2401 gagtactagg tatttttggt acaaagttaa ctaaaaatac ctttgcgaaa ggtatttcta
     2461 aagcagtacc agttgtggga ggcgttgttt caggaggcat caattacttg tctatgaaac
     2521 caatggcaac gaagcttcaa acagagttag ggaaaaatgt agcgtataca gaaaaaaatc
     2581 tccaacaaga tttagatatt ttagaaggtg aatttgaaga agtatcttct gatatcgtgt
     2641 cttctgatac agttctttct caattagaaa gactttctca cttgttggaa acgaatatga
     2701 ttacagaaga agagtttcaa caattgaagc aagagctgtt aaagaaataa ttatttagaa
     2761 aaagcaacca cttatacggg tggttctttt tcttgaatga attcaaattt attattagga
     2821 gaagaaaaag gatgaaagca aaacgattat tcctattagg cacgattctt ttacttggag
     2881 gatgttctac tgtctctcaa aatgaacaaa agaaacaagt ggattcaaca acttatgaaa
     2941 ctgaaacaac aagaaaggaa tcttctacaa aagaaataga aacaactact caagaaacta
     3001 aaacaacaga gtcttctagt aaggcaacga gttcagatac gagtgatttt caaataaaac
     3061 aagaattatg ggatacaaat aaagcatctc aattagcaac ttttgtccca caatggggaa
     3121 agacattagg tcaagaatat aaaagctaca atcctcagaa caatgtaagt ctgtatggta
     3181 cgcctttacc aagtgcagtt attgatggaa attggaaaat ggcaatcaat gaagcaccag
     3241 ttactgtaca atggtcagaa gatggaactg gaaatgcagg atttaattta gtagcagttt
     3301 attctgatgt agaaactgga gaatatttag ggcagcatgt ttactttttc ggttttcaaa
     3361 atggacaacc taaagtttat ttaacgcaac aaaatcaagg taatgaaaat aattatctgt
     3421 actttaacga aactcaaaat caacagctaa aacaaggatt tacagatatc attaatggac
     3481 aaactcccca aacaccagta gtagaagaat ctactcaaca agcaacaact acaacgacac
     3541 aatctacaag tagttcagga atttcaaatt gggaagctgc taaagaaact gtactaaata
     3601 ataaagcgtt atggatttat gagtctccag ataaaaatga agcaaatact aatattattg
     3661 aatccaccca atatagaaaa gatttaatgc aagatgaaaa tggagaattt tatccagttg
     3721 atatttctac tgatgatatt ggaaatacgg taatgaatgg aggaaaacaa ttccgtgttt
     3781 ataca
//
LOCUS       DAZULG010000173         3720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000173 DAZULG010000000
VERSION     DAZULG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.178"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..355)
                     /locus_tag="ACG9CY_002715"
     CDS             complement(<1..355)
                     /locus_tag="ACG9CY_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939165.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(615..1163)
                     /locus_tag="ACG9CY_002716"
     CDS             complement(615..1163)
                     /locus_tag="ACG9CY_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939166.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1164..2021)
                     /locus_tag="ACG9CY_002717"
     CDS             complement(1164..2021)
                     /locus_tag="ACG9CY_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4939167.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2307..2593)
                     /locus_tag="ACG9CY_002718"
                     /pseudo
     CDS             complement(2307..2593)
                     /locus_tag="ACG9CY_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2583..2912)
                     /locus_tag="ACG9CY_002719"
     CDS             complement(2583..2912)
                     /locus_tag="ACG9CY_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4939168.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2989..3261)
                     /locus_tag="ACG9CY_002720"
     CDS             complement(2989..3261)
                     /locus_tag="ACG9CY_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4939169.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3261..3524)
                     /locus_tag="ACG9CY_002721"
     CDS             complement(3261..3524)
                     /locus_tag="ACG9CY_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4939170.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1092 a          754 c          473 g         1401 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat actctatttg agagtcatct aattggctca
      241 taatcctttc tttttggtta gtatctgcag ttacttgttt agtatgagcc atgaacccaa
      301 acatactcac cccaacaacc aatcctaaat atattttctt tttaaacttt ttcatctaca
      361 tctcctccta atagttttat tttagagtta gctctattac ttatttacta actcaacaac
      421 agattatcat acacttgtta atgaaatcac aatatctcat taaaacacac cctaaaatca
      481 taaccgttcc tatgctcatt ttttggattt tttaatcacg taaccagaaa aataaatccc
      541 ctctatttca cgcaaatcaa agaagaacta agtaactacc gtttaaaggt agttacttag
      601 ttcttctttg attattatct aactttatta ttcattcccc accctttgcg cagtgagtct
      661 tcggtatctt gttttatctg actggtagtt cttattattt ctccacgatc atctaattct
      721 ggaacaattg gatatttttc tctgaacaag tctagttctt tcaccacgtc attaaacaaa
      781 tcttgcttat cttcttgcgt aagattctgt ttaatatact catctaaagc ttttctaagc
      841 aattcatctc ttgtcatatt agtctcatac ttttctacta aaactctgga agtcgcattt
      901 aacaaagtcg acagttttaa aggatgcttg gtggaataaa ccttatcaaa ctttctgatt
      961 ggtgcgccaa ctttttttgt tcttcctcta ctctgtcttt caacattgga ggaagttttt
     1021 atttcttgaa cttggtgcac ttctaattgc ttattttcag atggtgtaat tgcttgctgc
     1081 tttttctttg cttctgcttc tttttttatt ttggcgagtc ctttgttgtt cccaaaaata
     1141 tcaagtcctt tgatgtcagc caattactcc acctccgcat accaattctc gtgttcttcc
     1201 aattctttca aaatactgat aaataattcg tggcttttgc gatcccattg ttttaagaga
     1261 cctttctttg tcttctccat cgtaatacct gatccgtcgt atcgttttaa gcgcgcattt
     1321 ttgagtacaa catttttaat caaatgttcc ccataaatct cttgggcttg tttatacatt
     1381 tcctgatcta ctgcgtcacc gctttccaac atgaaaggaa taacaccagc aacatataaa
     1441 tttgtatcaa attctttcac aaaaaagtcc atatactcaa gatattgcat cgcatttctc
     1501 atcgatttta cttgggtctg taagacgacg attgcccaat ccgcaatgta catcgcggat
     1561 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
     1621 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
     1681 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
     1741 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
     1801 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
     1861 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
     1921 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
     1981 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
     2041 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
     2101 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
     2161 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
     2221 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
     2281 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
     2341 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
     2401 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
     2461 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
     2521 tctttcaatg gcttctcttt tagcttttta aatacttaat gacatgtttt gaaggaacga
     2581 tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt
     2641 gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct
     2701 acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta
     2761 aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac
     2821 tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt
     2881 gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta
     2941 attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc
     3001 gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta ccttataaac
     3061 aacccggtgt tgtctattca atcttctaga atacctacct tcatgtgtag gttttaactt
     3121 ctcaaatgat tgggtaggga gatagggatc ttcctttaaa gtctgaataa ccttctcaaa
     3181 ttgaggtttc aaattagact gtttgatttt ctttaaatca gctttagccg agtttttaat
     3241 catcacagaa tagttattca ttataagtta tcccaatcaa tttcatcaac attagtaaat
     3301 ccactatcat ctttttctcg cttacgaaca acacccaaag tgcctatttg ctcaagtaat
     3361 agcgtttctt tgattgcttt ccaatcatcc aaaccaataa gcaccgcatt attttcactg
     3421 cgctcactaa tgatttcaat ttccgtatga ttctcattta catctttcaa caacttataa
     3481 aaatttttcc gtgctgagct aggatttaag actgtttttg ccatatctac tccccctcta
     3541 atttatatct ccattctaac gtacttaatt aagtacgtaa attcgattgt tttgtttaaa
     3601 ttggagtacc aaaacagcat tgtaaagtgt tatccgtttc ctttataccc gtggtgctag
     3661 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
//
LOCUS       DAZULG010000174         3699 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000174 DAZULG010000000
VERSION     DAZULG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.179"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..1966
                     /gene="tdc"
                     /locus_tag="ACG9CY_002722"
     CDS             89..1966
                     /gene="tdc"
                     /locus_tag="ACG9CY_002722"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4939171.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            2209..3630
                     /gene="tyrP"
                     /locus_tag="ACG9CY_002723"
     CDS             2209..3630
                     /gene="tyrP"
                     /locus_tag="ACG9CY_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS4939172.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
BASE COUNT         1169 a          667 c          784 g         1079 t
ORIGIN      
        1 tcaggccaaa acttagttta cagaatagaa gaacaggtgt aatgtagggc tcgacaaaat
       61 ttaaatattt ttttaggagg gtttcactat gagtgaatca ttgtcgaaag atctaaacct
      121 aaatgcccta ttcattgggg acaaagctga gaacggccaa atctataaag ctttgttgaa
      181 tgaattggtg gatgaacatt taggctggcg tcaaaactac atgcctcaag acatgcctat
      241 tattacgcca gaagaaaaaa gcagtgctag ttttgaacac actgtgaata aaacaaagga
      301 tgttctatca gaaatttcag cgcggatgcg tacccattcc gttccttggc ataatgccgg
      361 acgttactgg ggtcatatga actctgaaac attgatgcca tctttactag cgtacaattt
      421 tgctatgcta tggaatggta ataacgttgc ctatgaatct tctcctgcaa caagccaaat
      481 ggaagaagaa gtaggaatgg aatttgcaaa attaatgagc tataaagatg gctggggcca
      541 catcgttgct gatggttctt tagcaaactt agaaggactt tggtatgccc gcaacatcaa
      601 atcattgccg cttgcaatga aagaagtgac acctgaacta gttgctggaa aaagcgattg
      661 ggaattaatg aacttgtcaa cagaagaaat catgaatctg ttagacagcg tcccagaaaa
      721 aatcgacgaa atcaaagctc actcagcacg tagcggaaaa catttagaaa aattaggaaa
      781 atggttggtt ccacaaacta aacactattc atggctaaaa gctgccgaca tcatcggtat
      841 cggtttagac caagtgattc cagtgccagt tgaccacaac tatcgcatgg acatcaacga
      901 acttgaaaaa atcgttcgtg gtctagcagc tgaaaaaaca ccaatcctag gtgtagtcgg
      961 tgttgtagga tcaacagaag aaggagcaat cgacggtatc gataaaatcg ttgcattgcg
     1021 tcgcgtatta gaaaaagacg gtatttactt ctacttacac gtagatgctg cttatggcgg
     1081 atacggccgt gcgattttct tagacgaaga caacaacttc attccatttg aagatttaaa
     1141 agatgttcat tacaaataca atgtctttac agaaaacaaa gattatatct tagaagaagt
     1201 acacagtgca tataaagcaa tcgaagaagc agaatctgta acgattgacc cacataaaat
     1261 gggctatgtt ccatattctg ccggtggtat cgtcatcaaa gatatccgca tgagagacgt
     1321 tatctcttac tttgcgacat atgtatttga aaaaggcgca gatatcccag cattacttgg
     1381 ggcttatatc ttggaaggtt caaaagcagg ggcaacagca gccagcgttt gggcagcaca
     1441 ccatgtctta cctttgaatg tcacaggata tggtaaatta atgggtgcat caatcgaagg
     1501 tgcacatcgt ttctacaact tcttgaatga cttgtcattt aaagtaggag acaaagaaat
     1561 cgaagttcat ccattaactt atccagattt caatatggta gactatgtat tcaaagaaaa
     1621 aggcaacgat gacttagttg caatgaataa attgaaccat gacgtatatg attattcttc
     1681 atacgtgaaa ggaagcattt acggtaatga gttcttaact tcacatactg actttgctat
     1741 cccagactat ggcaacagcc cattacaatt tgtgaaccaa ctaggattct cagatgaaga
     1801 atggaaccgt gccggaaaag taactgtatt gcgtgccagc gtaatgacac catacatgaa
     1861 caaagaagaa cactttgaag aatatgctga aaaaatcaaa gcagctcttc aagaaaaatt
     1921 ggaaaaaatc tacgcagacc aattattggc aagcgaagca aaataagcaa ggtaatcatg
     1981 ttagaaaaca gcttgaatac taactgtttc tgacgcatgt ttggatctga agagcaaggg
     2041 gagacctaag ctcttcgatc cctttgtttt gaggttgtct ttgaagaaac ccttcaagca
     2101 ataaactcaa agaatcccct caaatattcg ctgaattgtt tcgtttattg ctgaaactac
     2161 ttcaaagaca ccgttatttt atccaaaaaa tcaaggagag tgtcaacggt gagtaaatct
     2221 gaagtaagta aaatgggtct tgggaccttt atcggtctga caatggcact gtgtgcaacg
     2281 gttcggagta ttcctacgtt agccgcagtc ggttggacat tgatttttta ttcaattttt
     2341 gcagttttgt tttttgccgg tccgatatca atggtttccg gtgagctttc cacgatgctt
     2401 cctcaagaag gcggtcctca gttatgggta aaaacagcac tgggcagcaa atggggattt
     2461 gtcgttgcat ggctattatg ggtgcaaatg ttcccaggta tggtaatggt cgcttctact
     2521 ttaggaccat tgttaggaaa cacatttggt aatgtagaac ttggcaataa tcactggttc
     2581 gttttaggtt gtattttagt tatctactgg atcatcacga tcttgaactt gaaattcgat
     2641 atggcaaaag tcggcgggaa catcggtgta tggctagggg tatatatccc agtagtcatc
     2701 atgttcgttt taggtgtttt ggcagccttc aaagtcggac tagtctcaaa cggctatcta
     2761 ggtgactttt catggtcaaa agctttccct gacttggaac atattgattc attgaaatac
     2821 ttagctggta tcacatttat ttttgttggg atcgaaatga gttctgttta catgcctcgt
     2881 ttgaaagacg caacaaaaaa ttatacaaaa ggcgtattta tcgctttgat cggattagtt
     2941 ttattgaatg tcatcaatgc gatgcttgta gcaaatgtcg taccagatgg aaaaatggaa
     3001 ttagcaaata tcacgcagcc tatcttgatt gactgtcaaa tcctaggctt accagaagtg
     3061 atcggaaata tcttcagctt catggtattt attggtgtgc ttttacaatt atcagcttgg
     3121 gttactggac catcaaaaac aatcatccaa gtagctcgtg aaggattcct tccaccaaaa
     3181 ttcggattcc ataaagaaaa caaatacggc gtatctagaa atgtcgttct aacacaatca
     3241 atcgttattt cattatttgc tttgctttat ggtgtaatgg atgacgtaag tgctgtattc
     3301 ttgacactta caaatgctac aactgttatt tattgtatcg tgtacatttt gattgctgtt
     3361 tcattgctta aaatgcgtaa gaaacatcca gaattcgaac gtccttaccg aattggtaaa
     3421 aacggaaacg gattagcttg ggtagtcagc tgcatgctga tcttctctat catcgttgtc
     3481 gtatttgcta cattaggtac agcgacattg agtgatgcat tattagtagc agctatcaca
     3541 gtggtcatgt tcgttattcc attgatcatc aatcacttca agaaagattc ttgggggatt
     3601 gaggtagaaa aaagtttaga agaaaaataa aaaaataagt ctttcagccg acgttttgtc
     3661 gctgatttag taggaggtcc tgccaagtga atagttttt
//
LOCUS       DAZULG010000175         3656 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000175 DAZULG010000000
VERSION     DAZULG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.180"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..936
                     /locus_tag="ACG9CY_002724"
     CDS             76..936
                     /locus_tag="ACG9CY_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFS4939173.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            948..1775
                     /gene="panB"
                     /locus_tag="ACG9CY_002725"
     CDS             948..1775
                     /gene="panB"
                     /locus_tag="ACG9CY_002725"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFS4939174.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1790..2638
                     /gene="panC"
                     /locus_tag="ACG9CY_002726"
     CDS             1790..2638
                     /gene="panC"
                     /locus_tag="ACG9CY_002726"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFS4939175.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2648..3043
                     /gene="panD"
                     /locus_tag="ACG9CY_002727"
     CDS             2648..3043
                     /gene="panD"
                     /locus_tag="ACG9CY_002727"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFS4939176.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            3245..3656
                     /locus_tag="ACG9CY_002728"
                     /pseudo
     CDS             3245..3656
                     /locus_tag="ACG9CY_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1420 a          419 c          728 g         1089 t
ORIGIN      
        1 acatactgta tatttgattt aatttttatt tttaagctca tatcgtaaga atgggacttg
       61 tagtctcact tctttatgga ggtgataatt atgaacgttg gttttattgg agccggcaag
      121 gtaggttgtt cattcgggaa gtatttccag gaacataaaa tacaagtaac aggattttat
      181 agtaaaagcg aagactcgtc attagcagct tcgaatttta catctagtaa acaatattta
      241 aacttaagag aattagtaga tgaaaatgat actattttca ttacgactcc tgatggtcaa
      301 attcaggagg tatggcaaga aattaaaaat tatcaaatca aaaacaaatt aatctgccat
      361 tgcagtggtg caatatcttc agatatcttt tcaaatatac aggactatgg tgcttatggc
      421 tattctgtcc atcctatgtt ttcaatatca gataaatata attcatacaa aaaattaaaa
      481 aaatctttta taacaattga aggcgatgaa aaatatgcaa agtttttatg tgatttcttc
      541 aaaaagttag gtaatagtac tgtaattgta tctaaggaga ataaatctct ttatcatgcc
      601 gcatcagtag tatctagtaa ccttgtgcta ggacttatta atacaagcgt tacatatcta
      661 attcaatgtg gatttactga aaaaatggca attgaagctt tatatccatt aattgaattt
      721 aatattagaa atataaaaga aaaaggaatt atagaaagtt tgacagggcc agttgaacgt
      781 tgtgatattt caacaattaa aggtcactgt gaagttttat cagatgaaga cagagcttta
      841 tatatgctat tatcaaaaaa tgtattagag atagctatgt taaaaaatat taatagagat
      901 tatagtgaat tagaaaagta tttaggagag agttaggaga gaaatagatg aaaaatacag
      961 cagttacatt taaagaatct aaattaagaa atgaaaaatt aacaatgctt acagcttatg
     1021 actattcaac agcaaaaata attgatgaag ctgggataaa tggaatatta gtaggagatt
     1081 ctcttggtat ggtatgtcta ggacatgaag atacactttc agtaacaatg gaagacatga
     1141 ttcatcatac tagagcagtt acaagaggag ctaagaacac tttagttgtt gcagatatgc
     1201 catttatgtc ttatcaaact tcagtatatg attcagtagt taatgcagga agattaataa
     1261 aagaaggacg agctcaggtt gttaaattag aaggagggat agaagtatgc gataaaatag
     1321 aggctatagt aaaggcttct atacctgtta tggcgcatat aggtttaaca cctcaatctg
     1381 ttaatgcatt tggaggattt aaggtacaag gaaaggacaa ggaagctgct aaagaattaa
     1441 taagagctgc taaagctgta gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc
     1501 caactaagct tgcagagtta atttctaaag agatatcaat acctacaatt ggtattggag
     1561 caggtgcagg gtgtgatgga caaattcttg tatatcaaga tatgttgggg atgtatagtg
     1621 attttacccc taaatttgtg aagaaatatg ctaatttatc agaagagatg aataaagcat
     1681 ttacaaaata tatagaggaa gtaaaagatg gagtttttcc aggtccagaa catgggttta
     1741 aaataagtga tgatgtttta gaaaaattat attaaaaggg tcaagaaata tggttatatt
     1801 taaaacagtg aatgatgtta gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt
     1861 aggattagtt cctactatgg gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc
     1921 atcaaaagaa aatgataaag ttgttgtaag tatctttgta aatccaactc agtttgggaa
     1981 aaatgaagat ttaggtagtt atccaagaga cttagaaaga gatatagaag tatgcacaag
     2041 aggtagagca acagcaatat ttaatcctga agttgaagag atgtattgtg acaatgcaag
     2101 tacttttgtt aatataactg gattaacaga aggtttatgt ggagctagta ggccaataca
     2161 ttttagaggg gtttgcactg ttgtaagtaa attatttaac ataataccag cagatagagc
     2221 ttattttgga gaaaaggatg cacaacagtt agctgtaatt aaaagaatgg ttagggacct
     2281 taatattgat atagacgttg tagggtgtcc aattataagg gaagaggatg gactagctaa
     2341 aagttctaga aacacatact taagtttaga ggaaagatct tcagctacta ttttaaataa
     2401 atctttaact ttagctaagg aagctttaaa taatggagag agagacagct taaaaataat
     2461 tgagataata agtaaaaata taaatactta taatttggca aagattgatt atgtagaagt
     2521 tgttgattct ttatcattac aaagagtaaa ttatattgaa aaatcagttt tagttgcaat
     2581 agcagtattt ataggaaaaa caagattaat agataatttt acatttgaat tacaataggg
     2641 ggctataatg aaagttacaa tgttaaaagg aaaaatacat agagctacag ttgaacaagc
     2701 tgagttagat tatgtaggaa gtattactgt tgatatgaat ttattagaag cagcaggaat
     2761 atatgaatat gaaaaagtac aaatagttga tgttaataat ggaaatagat ttgaaactta
     2821 tacaatagct ggagagagag gaacagggat gatttgcttg aatggagctg cagctagatg
     2881 tgtatcaact ggagataaaa taattttaat ggcatattgt gaattaaatg aaaatgaggt
     2941 taaggatcat agacctaagg ttgtttttgt cgatgataat aataaagttg aaagagtaac
     3001 atcatatgaa aaacatggaa ggttatcaga tataattctt taggttttgt aatgttattt
     3061 atatatagct agactattat tgaaatattt attgattact aattggatag atgtcaataa
     3121 agtagacaca aaaagagaag atacagactg tagacaaagt ccattttatg tgaatcggct
     3181 acactagact agacagaaaa aataaggtgt gtagaataat aaaaaacaca ctggaggatt
     3241 tttcatgtca agacgtcaac gaagaaccta ttcaaaagaa ttcaaacaac aaatcgtcga
     3301 tctctatctc gctggtaagc ctcgcgcaga aattattcga gagtatgagc ttacgccttc
     3361 ttctttcgat aaatggatga agcaagcaca atcaacgggc tcattcaaaa aaagagacaa
     3421 cttaacacca gaacaagcag aattgatcgc actaagaaag aaaaataagc aactcgaaat
     3481 ggagaatgat attttaaagc aagcggcgct gatattcgga cgaaaagaca agtaattgat
     3541 gccaacaagc ataaatattc catatcagcg atgtgcaaaa ttctaaatat ttctcgtcag
     3601 acctactatt atcaagcgaa accgatcgaa aatgagtccg acttagaaga gatcgt
//
LOCUS       DAZULG010000176         3613 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000176 DAZULG010000000
VERSION     DAZULG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.181"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..1046
                     /locus_tag="ACG9CY_002729"
     CDS             165..1046
                     /locus_tag="ACG9CY_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4939177.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1059..2669
                     /locus_tag="ACG9CY_002730"
     CDS             1059..2669
                     /locus_tag="ACG9CY_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4939178.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2746..3405)
                     /locus_tag="ACG9CY_002731"
     CDS             complement(2746..3405)
                     /locus_tag="ACG9CY_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4939179.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
BASE COUNT         1011 a          689 c          709 g         1204 t
ORIGIN      
        1 caagctagca attccttgtt tatgaattca ttgacagaaa tttataaaaa gaatattatg
       61 tactaaaata ataaaaaaaa gttttataaa aaggtataaa acctttttat taaactattt
      121 aatcttgttt agtatatatt tttagaaaaa ggagtggggt ttttatggca gaaatcgtca
      181 aagtcacaca tttgaaaaag agctttggtc gtttcgaagc tttaaaagat gtctctttca
      241 ccgtacattc aggtgaagtc gttggattta tcggaccaaa tggttctgga aaatccacga
      301 cgattcggac acttttaggg attataaaaa aagacggcgg aacggcaacg atttttgggc
      361 aagatgtatg ggaagatagt ttatctatcc atgaacgtct ttcttatgtt ccaggcgata
      421 tctctttgtg gggaaatttg actggaggag cgatcatcga tctcttcatg aagcttcatg
      481 gaaaagggag tgctcaaaaa aaagatgaac tgattcaacg tttctctctt gatcctaaga
      541 aaaaagcaaa aaactattca aaaggaaatc gccaaaaagt gggcttgatt gctgctttgg
      601 ctgttgattc ggatctctat ttatttgatg agcctacttc tggtttagat cctttgatgg
      661 aaacgatttt ccaagaagaa gttgaaaaat tgaaagaaca gggcaaatcc atcctacttt
      721 cttctcatat tctcagtgaa gtcgagcgac tagcggataa agtcgtgatc atacgcgaag
      781 gaacactcga tgaagaagga acactcgatg aattgcgtca tttgactcgt tcgaccatca
      841 ctatagaaac agaagcagag aatagtcact tagcacaatt gaacggtgtc catgactttg
      901 tgcaaaaagg tcacctcgct caatttgctg ctgataacga agctttcgat gtgattttag
      961 ctgaagcgtc caaattaggt gtgaaaaaat ttgaatctat tccaccgact cttgaggatt
     1021 tattcatgag acattatgaa ggatgaggag gaagcgaaat gactgataaa ttttcacgct
     1081 ggtccaccct tttttctcaa aaccttagcc gtgactggaa aaagatcctg atatggctag
     1141 tcggtgtcac tgctttttct ggtggttttg tccctgcgtt tgaagaattg acttccggac
     1201 aaggattagc tggattatac gaaaccttgc aaaacccggc aatgattgca atggtcggac
     1261 caactccagc ttcgaatgct gctgagtata ctttaggagc gatgtatgca aatgaaatgt
     1321 tgcttttttg cagtttgttt gctgctgtga tctctgtttt acacgtggtg agccaaacga
     1381 gaaaaaaaga agaacttggt ctgattgaac ttattcgttc ctttcgagtc ggacgtcaag
     1441 caaactcttt agctgtcatc gctgaaagta ttctgatcaa tttgctttta gcaatatcga
     1501 tcagctgtac gatgattttt ttccaagcgg attcgatcac tgcagaaggt gcttggcttt
     1561 ttgggctatc tgtaggtagt atggggattt tggggagtgc cgttgcactg tttgctgcac
     1621 aactgatgcc aagttcagcc agttcgaatg gtctgtcttt agcgttttta ggtggacttt
     1681 accttctacg agcaataaca gatgtagctg attcctccct ttcgatgtgg aatcctttag
     1741 gctggtctta tttgacctat ccatttacaa ctaacaactg gattcctttg ctttatttgt
     1801 ttttgttcag tctaatcttg ataatcagtt catttgtttt agaggaacat cgagatttga
     1861 acaccggcta tatccccgaa cgtgaaggaa aaccgtatgc taaacgttct cttttatcct
     1921 tgcgtggttt attattctat ctaaataaag ggatgacaat cagttggctt atcacttttg
     1981 gtttgatggg ggcggcatac ggctctatct acgggaatat gcaacagttt ttagaaagta
     2041 acgacttaat gaaacagatg tttatccaat ctggcgtctc aattgaaaaa aactttacaa
     2101 gcacgatcat ggtagtcttg atttgtctag ctgctatttt accaattgct agcatcaatc
     2161 gtttatttac agaagaaacc cacggacgta tgaatcagct gttcgcaaca aaaatttcta
     2221 ggaaccaact ttattggacc aatatgttac tagctattct atttgctata ttggcaatcc
     2281 tgattagttc tggtgcacta gggctttctg ctttggcttc gatgaaggat gcgaaagaca
     2341 tggatctatt gatgtttctt aaagccgggt tcaatttcct gcctgccgtt ttagtgttta
     2401 tcggaattag tggctttcta ttcggatggc ttcctcgaat cggtaaactt gcttatatct
     2461 acttagctta ctcctttgca ctcaattact ttggcggaat tttggattta ccagaatggt
     2521 tttccaaaac agctgtccct agctgggttc cacggatgcc aaacgattct tttgatcttt
     2581 ccgtgttcct tacgttatca tgtatcagcc ttgcactgtt cgtccttggt tctattggtt
     2641 ataggcgaag agacttacta gaaggataat aaaaaagcat gtacaatttc atgctagcct
     2701 ccaaatctgg gtcaaaaatc caacatttgg aggtcatttt tttatttaca agtcttctaa
     2761 ctgctcttta tcaatgatcc agacttctcg attctttctt tttacataac cattctcctc
     2821 taataattta aattttctag ataaagtttc gggggtcgta ccgataaatg cagcaagttc
     2881 tttcattttc attggcagga aaaagtgatc cttccctgtc gctttagcta agtcaagcag
     2941 atagacagcg actcgttctt ctactttttc catcatcaaa aaagttgtct gctgctgtac
     3001 ttttgttaat ttctcaacgt tgattgctaa aagtttcaga ctaagggcgg gataatgcag
     3061 taagagttcc gagaaatctt tttgtctcaa tatgcaaatt tccgtttttt ctagtgcttc
     3121 gccaaataaa gactcatttg gaacgcctag caactggctc tctccctcat atccgcccgg
     3181 ctctattact cgtaaaagct gttcctttcc gctagcagat aattgataca ctttcatact
     3241 gccggaggcc acgatcacta attgaggttc tccattcggc gcaagaatct gttctccttt
     3301 ttccacatag tgatgtttga caagtgcgtg tactttttct tgatcttgct gtactaagtg
     3361 attgaacaaa ggaacaaatg aaacacataa atgatcatga ctcataatct ctcctccttc
     3421 taaacagcta tttctaataa agaaacataa cttcccttat tccaactgat aattttcttt
     3481 tatcatagca taaacgtcaa gcgaaccaga taccagcaaa tagagtggat ttctttttgt
     3541 cccttgcttt attttccttc ttttgagacc ttaagtaatt gattcctatc aattttttaa
     3601 atatttactt ttt
//
LOCUS       DAZULG010000177         3582 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000177 DAZULG010000000
VERSION     DAZULG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.182"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..269
                     /locus_tag="ACG9CY_002732"
     CDS             <1..269
                     /locus_tag="ACG9CY_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFS4939180.1"
                     /translation="PNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKN
                     YDYIGDDVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            259..879
                     /locus_tag="ACG9CY_002733"
     CDS             259..879
                     /locus_tag="ACG9CY_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4939181.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            1075..2175
                     /locus_tag="ACG9CY_002734"
     CDS             1075..2175
                     /locus_tag="ACG9CY_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4939182.1"
                     /translation="MAKYYNKKILIMANNDDGLYRFRGMLIKNLVDQGNEVIVSLPKG
                     KNIPKLIELGCQIIETPIDRRGINPKTDFKLFFNYCNILKKIKPDMVIAYTIKPNIYG
                     GLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFLYRLAAKKSKVIFFENFENMKTFQ
                     TLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFLFIGRIMKEKGVDELFEAMKNL
                     YSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGYQSDIRPFVERAHCFVLPSWHE
                     GMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLCERKNSCSLYNSMNKFLKLSYE
                     QRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            2182..2577
                     /locus_tag="ACG9CY_002735"
     CDS             2182..2577
                     /locus_tag="ACG9CY_002735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4939183.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            2574..3575
                     /locus_tag="ACG9CY_002736"
     CDS             2574..3575
                     /locus_tag="ACG9CY_002736"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4939184.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDIIQNLSDEYYTRAICKCKYKDNWKGISAKFALKNKWVFLIYIYNKLIKR"
BASE COUNT         1415 a          404 c          664 g         1099 t
ORIGIN      
        1 aaccgaatta ttggttaagt gtcataacaa taaagggaat aaatgcgaat catcttattg
       61 atgagttaga caaaaataat atagaagccc gcccagtatg gaaacctatg catttacaac
      121 ctgtttttaa aaattatgat tatattggcg atgatgtagc agaaacttta tttgacttag
      181 gagtatgttt accatcagat acaaagatga cagatgaaga tttaaaatat gtagtatcta
      241 cgattaaaag agtgatcaat gatgcgtaaa aaaactttct atcagctttt tgggaaaaga
      301 attttagata tcctattgtc aggaattgct ctcatcgttt tatcgccgat aatcttaatt
      361 gtaggttttt tagtaagaat taaacttggt tctccaatta tcttcaaaca agaacgacca
      421 ggaaaaagtg aaaaaatttt ttccatgtat aaatttcgta caatgactga cgaaagagac
      481 cacaatggag aatatttacc tgatgaaatt cgactaacta aatttgggaa aatgcttaga
      541 gcaacaagtc tggatgagct acccgaactt tggaatattt tgaaaggtga tatgtcgatc
      601 gtaggacctc gtccattatt agtggagtat ttgccactat atagtgagaa gcaacggaaa
      661 agacacaacg taagaccagg attaactgga ctggctcagg tcaatggaag aaatgctatt
      721 agttgggaag aaaagtttga tttagatgtt tattatgttg ataaaattag tttctttaat
      781 gatcttatta ttataattca gacatgtaaa aaagtaatca agaaagagaa tattaataca
      841 atcaataaca atatcccaga aaaatttaaa gggagttaaa tacagcagct taatgcaagt
      901 tttgtgactt gcattatatt taggtataaa tcaaagcgag tatattagaa ttatataaca
      961 tatgttaaaa aaacgtatag ctaataaaag tatgatttta aaaatttgtg tagagtttta
     1021 aatatactgt ttcaatacga gaaaattata aaaaagtata aggaaaagag agttatggca
     1081 aagtattata ataaaaaaat tcttataatg gcaaacaacg atgatggtct ttaccgtttt
     1141 cgaggaatgc tcataaaaaa cttagtagat caaggtaatg aagttattgt atctcttcca
     1201 aagggcaaga atattcctaa attaattgaa cttggatgtc aaattattga aactccaata
     1261 gatagaagag gaattaatcc aaaaactgac tttaaactat tttttaacta ttgtaatatt
     1321 ttgaaaaaaa taaagccaga tatggtcatt gcttatacta tcaaaccaaa tatatacggt
     1381 ggattggcat gtcggttaag aaaggtttct tatgcattaa atataaccgg attaggaacg
     1441 gcatttcaaa aaaagggggt tttgaaaagt cttattgtat ttttgtatag attagcagct
     1501 aaaaaatcta aggtcatatt tttcgaaaat tttgaaaata tgaaaacatt tcagacttta
     1561 caaattgcta aacaaagtaa atacaagcta ttaaacggtg caggagtaga tgtagaacac
     1621 tttagttatt tagaatatcc taaagatgag caagttcatt ttttatttat tggaagaatt
     1681 atgaaagaga aaggtgtaga tgaacttttt gaagcaatga aaaacttata tagtgaagag
     1741 aaaaattgtg tacttgatgt ccttgggtat tttgaagaag attacggcat aaaaattgaa
     1801 aaatacgaaa aagagggatg gttaaaatat catggatatc aatctgatat tcgtcccttt
     1861 gtagaaagag cacattgctt tgttttgcct agttggcacg aaggaatggc aaatactaac
     1921 cttgaatcag cagcatcagg gagaccaatt attacaagtg atatcccagg gtgtaaagaa
     1981 gcagttattg aatcgaagtc tggttattta tgtgaaagaa aaaatagttg tagtctctat
     2041 aattcgatga ataaatttct aaaattatca tatgagcagc gcattaacat gggaaaaatg
     2101 gggagaaatt atatgattaa aaaatttaat aagaagaaag ttgtcgatga aacaattaaa
     2161 tcattattta gttaaaaaga tatgttttgc aaagtatgtg atagaaaaga gggattctgt
     2221 ggcttcagaa tagatatcaa agaattggca aaagtttctg atattttttt atttactaca
     2281 ttacaagaag gaatgccaag atctatgatg gaagctatgg caagtggatt gccatgtata
     2341 gcaagtaaaa tccgaggtaa tgtagacctt ttagtggaag gaaaaggtgg atatcttgtt
     2401 ccagctaaag atgcagaatt agtagcagag tgtttgagag aactaatcaa taataaatct
     2461 cttagaaagt atatgggaaa tgagaacctt tccagaatta aagaattcga ggttagtatc
     2521 gtaaaaaaaa gaatcaaaga aatatatgaa gaagttatag gtgagagtgt aaaatgaaaa
     2581 atccaatgtt ttcaataata gtacctatct attgcgttga aaaatatttg aaagattgct
     2641 tagattcaat aagaaatcag acatttaaaa attttgaagc aatactagtt gatgacggta
     2701 gtcctgacga atgcccagaa atatgtgact attattccca aatagatgga cgtttaaaag
     2761 taattcataa gaaaaacggt ggaataatag atgcacggaa aacaggagtc actagtgcta
     2821 tgggagagta tatagttttt ctagatggtg atgattggct tgcaaatact tatttagaaa
     2881 aatatgcgga agtaattact aagtacaatc cagatataat atgtagtggg tattttgcat
     2941 cacgttccaa caaaattaga aaaagagaat tgtcttgctc gtttggatat tatgacaaag
     3001 ataaaattat aaatttgatt tatcctaaat taattgagga tgaacatgga gagtatttta
     3061 gaccacaatt atgggcaaaa gcttttagaa ctactctgta caaggaacaa caacaagtat
     3121 ctgggcaagt tagtgttggt gaagatcatg catgtacaaa accatgtatt taccatgcaa
     3181 attcgatata tatgatggaa aaatgtttat attattacag aataaacgaa aattctataa
     3241 caaaaaaacc acaaccatat tattgggaag gaccaaaggc tattggagag cattttgaaa
     3301 agcagattga tatgaataag tatgattttc aagatcaagt atatagaaat gttacacaca
     3361 atttatttaa tgttgctgtt tcacaattta ataaacagga caaatattta aatataaaaa
     3421 aagatattat acaaaatcta agtgacgaat actatactag agctatttgt aaatgcaaat
     3481 ataaagataa ttggaaagga attagtgcta agtttgcatt gaaaaacaaa tgggtatttt
     3541 taatttatat ttataacaaa ttgataaaga ggtaataata at
//
LOCUS       DAZULG010000178         3566 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000178 DAZULG010000000
VERSION     DAZULG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.183"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..1883
                     /gene="yfcC"
                     /locus_tag="ACG9CY_002737"
     CDS             144..1883
                     /gene="yfcC"
                     /locus_tag="ACG9CY_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS4939185.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2040..3224
                     /gene="iadA"
                     /locus_tag="ACG9CY_002738"
     CDS             2040..3224
                     /gene="iadA"
                     /locus_tag="ACG9CY_002738"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS4939186.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3335..3538
                     /locus_tag="ACG9CY_002739"
     CDS             3335..3538
                     /locus_tag="ACG9CY_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4939187.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1141 a          593 c          826 g         1006 t
ORIGIN      
        1 acataggaga aaaaaaacaa aatagaaaaa agtgattttc taataaatta ttcatgtgga
       61 aatgtcattt ttactgtata attcatttga ttatccattt tttattcatc aaaattactg
      121 aaaaaagaaa ggaagaaata aatttgaaga aaacaaattc ttcaggaaaa ccgaaacgcg
      181 acttcactaa attatcgaca ccccatacgt atgtcattat ttttggtgta gtcatctttg
      241 catggatact gacttttgtc gttccagcag gaaagttcag cacgcaagat attgaataca
      301 aagatgcaaa tggcgaaaca agtactcgta ctgttttacg tcaagattcc ttccgttatg
      361 cttacgagct ggataagtcg tatgtgtttg atcaattaga agaactgcaa gatcatccag
      421 cagaacgtga aaaactggat gttccggaaa aaggattgga aaaagtcatt gctgacggag
      481 aaaaaaatct tacgcaggaa aaactagatg aaatctcctt gacagatgat gtgctttacg
      541 atcagtacgg cgaaaatatc tatgatactt ctaaaaaatt gcataaaaca gccaaaatat
      601 ggggaacaga tgattttggc ggctttggtt tcttaaattt tgtttttgaa ggactagtat
      661 ctggagataa gtatggatct gctgtaggga tcgctgcatt gatccttgtt gttggaggtg
      721 cttttggtat tattatgcgt accggagcga tcgacgcagg tatctatgcg tttatcagta
      781 aaactaaggg actggagcgt ttagctttac cgctattatt ctttgctttt tcattcggtg
      841 gcgctacatt cgggatggca gaagaggtaa ttccattctc aatggtgatg gttccatttg
      901 tcattgcgtt aggatatgac tcgatcgtag cagtaaccgt gacttatgtc gcatctcaag
      961 tcggaaatgc gacctcttgg atgagcccgt ttagcgtagc ggtggctcaa gggattgctg
     1021 gaatccccgt gttgtctggt gcgacattcc gattgattat gtgggtagtt gttactgcat
     1081 tagccgctgg ttatatgatg atatatgcag aaaaaatccg taaaaaacca gaaagttcgt
     1141 tgacttacaa atcagatgat tatttccgtt ctcatatcaa aaagacatct gatgaaaata
     1201 aaaaattcat gcttgggcat aaattgatct tattagagat gttagttgtc cttgtctgga
     1261 tcgtttgggg cgtgactcaa aaaggctatt acattccaga aatcgcttct caattctttg
     1321 tgatgggatt agttgctgga atcattgctg ttcttttcaa attagatgga atgaaagtca
     1381 atgacatcgc ttcttctttc caatctggtg cagctgattt agctggtaca gcaatcgtag
     1441 taggtatggc caaagggatt cttcttgtct tgggaggatc tgatgcatct gttccttcag
     1501 ctttgaatac gattttgcat gggatcggga cggcgttgac cggtgttcca gctgtcattg
     1561 gtgcttgggc gatgtacatc ttccaaagtt tgttcaactt ggttgttaca tcaaattcag
     1621 gacaagcagc tttgactatg ccaatcatgg ctccactggc agatttagta ggtgtatcca
     1681 gacaagtagc agttcttgct tatcagcttg gttcaggttt tatggacgct tttacacccg
     1741 tatcagccag cttgattggt gtgctaggtg ttgcacggat cgaatgggca aaatgggcaa
     1801 gattccaaat caagatgcaa ggattcctgt ttgttttagg aactattttc atcatgattg
     1861 ccattgcaat aggtctgcag tagtactgga gacctgtaaa taaaaaagta atccaaacag
     1921 tagataagga ctccaagaac cgtcatttta cggtaaaatc aaaggagtaa agatcctatc
     1981 gtttggatta ttttttaaga tcctatcatc agttatactt aaagaaagag gaaaaatgta
     2041 tgaaaatcat tcgacaaatt gaagtgtttg caccagatta cttaggaaaa atggatgtac
     2101 tgattgccgg tgggaagatt ttggctgtag aagaacagct agaaggcggc tacgaaggag
     2161 tggaagtaga agaactttca ggtgagggaa aagtactaac cccgggtttc attgactgtc
     2221 atttccatat tttaggtgga ggaggagaag gcggttatca aaatcgaacg ccagaagtca
     2281 cattgagcca attgactaca gctggtgtaa cgacagttgt cggttgtttg ggaacagacg
     2341 gagaaggtag agatatgact gcgttgatca gcaaagcgaa aggtctggaa gcagaaggaa
     2401 tctccactta tgtatacgaa ggttcgtacc gattgccggt aaaaacagta acagattcta
     2461 tcatcaaaga ctttttgaca attgataaaa ttatcggaat cggagaaatc gcagtatccg
     2521 atcaccgttc ttctcagcca agttttgaag agttcgctcg tgcagtagca gatgctcgtg
     2581 taggaggcat gttatccgga aaagctggga tcatcaatgt ccacttaggc ggaggaaaaa
     2641 gaaagctaga gctattgaca cgaatcgtag aagaaacaga aattccgatc acacaatttt
     2701 tgcctactca tgcaaatcgc acaccagaat tatttgaagc ttgcgtggca tttgccaaac
     2761 gtggtggcac gattgacttc actgctagcg aagatcctga tttttgggag aagacagatg
     2821 gtgaagttcg cttcagcaaa gcactgaaac gattgatcga ggaagatgtt tctttagata
     2881 atttcacgat gacctcagat ggacaaggaa gcttgcctta ttttgatgaa aacaatcatt
     2941 tcctagggtt aggagtagga agtgccaagg cattgctagt agggatcaaa gaagctgtgc
     3001 aaaaagaaag tatccctttg gaaatcgcct tacgagctat aaccagtaat ccagcacgta
     3061 tattgaaatt ggataaaaaa ggaaaaatcg aaattggtgc tgatgcagat ctgtgtatct
     3121 tggataaaga aacgcttgat attgatacag ttatcgctaa aggagaaata atggtccaag
     3181 aaaaagaagt caaagtatgg gggactttcg aaaaaagttt ttaatcaaca gctctaacta
     3241 tcttttttag aaaacgagcg ttctgtttgt ctgtatctgt tgttgatgtt accctgaagg
     3301 tagatcaata aacaaactaa gaggaggaag cacaatggca gatttaaaag gacgttctaa
     3361 agatatgaaa gataaagtag tcggtgaagg aaaagaagca tacggcaaag caacagacga
     3421 caagagcaaa gaaacagaag ggaaagcaca atcactaggt gctgacatca aagaaaaagc
     3481 tcgaaattta ggcgacgaag tcaaagacaa gttttctcga aaagacgaaa aaaaataaaa
     3541 accacttatt actaattgaa tcaagc
//
LOCUS       DAZULG010000179         3534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000179 DAZULG010000000
VERSION     DAZULG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.184"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..905)
                     /gene="nth"
                     /locus_tag="ACG9CY_002740"
     CDS             complement(228..905)
                     /gene="nth"
                     /locus_tag="ACG9CY_002740"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS4939188.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(912..1610)
                     /locus_tag="ACG9CY_002741"
     CDS             complement(912..1610)
                     /locus_tag="ACG9CY_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4939189.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1878..3062
                     /locus_tag="ACG9CY_002742"
     CDS             1878..3062
                     /locus_tag="ACG9CY_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS4939190.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3068..>3534)
                     /locus_tag="ACG9CY_002743"
     CDS             complement(3068..>3534)
                     /locus_tag="ACG9CY_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4939191.1"
                     /translation="KEKEGVEMDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNE
                     LEVIEHIGKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQL
                     TPIGQKIRNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          901 a          712 c          598 g         1323 t
ORIGIN      
        1 tactggactt attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc
       61 aataaaaata acaactttta tttttatcgc aataaattgt aataagttac catcctagct
      121 actgagtatg tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat
      181 aataacaaga taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt
      241 ttctttttta gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat
      301 agcaaaggac atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa
      361 atcagtgtat gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct
      421 tctacttcca tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc
      481 acgtgggtat ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct
      541 gtctttctgc ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca
      601 tcgaaacgtt caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac
      661 aaaccaatcg tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg
      721 ggtgtcggaa aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc
      781 tgggcactta agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca
      841 tgtgcttcgg gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt
      901 aacatattcc gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt
      961 tgagctgact gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct
     1021 tccacttgtt gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg
     1081 taattaaaac tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc
     1141 ggctgataat ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc
     1201 gggcgtccaa attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt
     1261 ttttcttcgt attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg
     1321 taaagattat atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat
     1381 ccatttgtta cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc
     1441 atagcaatca tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt
     1501 agaacaaata aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga
     1561 ttagatatga ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct
     1621 ccgctcaatc acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat
     1681 ttacttcact gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt
     1741 tttgatgcgt atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag
     1801 aatgttgaca aggaaacaaa attactatac actatttttg tttccaagtt aacattattg
     1861 cataaaggag tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg
     1921 tctctgtctt attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa
     1981 cgctgcctta ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg
     2041 tttatgctct agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg
     2101 ggcgtcatcc gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg
     2161 gtttaggaaa ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag
     2221 gagaaatctc caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca
     2281 aagtttttgg atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag
     2341 ggggtctatt agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt
     2401 tcttaaatgc cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt
     2461 cagtagattt aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga
     2521 tttcatccgt tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc
     2581 ttcaatctat cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg
     2641 gtttaagctt ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc
     2701 gattgctcaa aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc
     2761 tcgcctatct attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg
     2821 ggattatctg ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat
     2881 ccaattctgt ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat
     2941 ctctatcgcg cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc
     3001 ttccgacggt attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat
     3061 agtagcttca tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg
     3121 tgtcaaattc ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt
     3181 gatggagttg ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga
     3241 tttctttttt attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt
     3301 ttgttagctt actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt
     3361 taggatcagg gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg
     3421 caaattcttt ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat
     3481 attcttctat tagcagttgt cttttatcca tttctacacc ttctttttct ttca
//
LOCUS       DAZULG010000180         3458 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000180 DAZULG010000000
VERSION     DAZULG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.185"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG9CY_002744"
     CDS             complement(<1..2232)
                     /locus_tag="ACG9CY_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4939192.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3197)
                     /locus_tag="ACG9CY_002745"
                     /pseudo
     CDS             complement(2904..3197)
                     /locus_tag="ACG9CY_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          969 a          765 c          548 g         1176 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctcccga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcact
//
LOCUS       DAZULG010000181         3269 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000181 DAZULG010000000
VERSION     DAZULG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.186"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(486..1100)
                     /locus_tag="ACG9CY_002746"
     CDS             complement(486..1100)
                     /locus_tag="ACG9CY_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4939193.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1706..2083)
                     /locus_tag="ACG9CY_002747"
     CDS             complement(1706..2083)
                     /locus_tag="ACG9CY_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939194.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2383..3264)
                     /gene="galU"
                     /locus_tag="ACG9CY_002748"
     CDS             complement(2383..3264)
                     /gene="galU"
                     /locus_tag="ACG9CY_002748"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4939195.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1001 a          668 c          499 g         1101 t
ORIGIN      
        1 aaaaaaataa taaacactta taaatatcca ttatctatcc ttcaattttt gtgtatttta
       61 tttctattag aaaaaggtat cctgttggac aattttgtta tatcaggtaa acttcattgc
      121 aataatgata acaaatgtac tataattact ttgtaaagag ttttggggaa gactctttca
      181 tcaacgtgta ttaagagaca agaaagaatc catatggatt ctttcttgtc tctttttcca
      241 ctacttttta tttttctgac actacatatt cttataactc atttctcata actgtgaaac
      301 aacttataaa aataacaaac tttaaacaaa acaagcttaa aatcaaagat gatccatctt
      361 aaaataacta aaacaaaaaa agtagtcgtt gttaatcctc attaataatc gttttctttt
      421 tttcaaacgc tccattttta tgtggaacat tattttttta aggaactttg tcaacatgtg
      481 gataactatt ttccacttgt gtcctggact agctgttttg ctgtaaaata caaaacttgc
      541 tgcagcaaaa agatcgttgc aagagaggct gttccgcaga tcgctggatc tagatttggc
      601 aaagtcaaga ctgcatcatg taaattggcg tggttgtcca aaaccaaatc agcaaattca
      661 aataatcgca aaccactgct atgttgcgat ttgatacttc gtgacaaatc aaatccagta
      721 acaatgatta atggatgacc attttctttg atccactgag ctagttcgat gattgccggg
      781 tttcttcctt cattcgaaag aagaagaaaa atgtcttctg atttgacaga aagctgatca
      841 ataattgcct gtcctgcacc ttctagcgtt tcatagacac ctccggctgg attttcaatc
      901 aatatacttg gtacaagatc cggggtttga tcccaaaatt caaatgctac accacttagt
      961 gtgcggctag catatatctg caatctacca ccttgattaa tttgtttcgc gatccaagtg
     1021 ccagcttgaa gcatcagcgg ctcttctatc cttaatttct cttccaattg gtgataatag
     1081 tcaaaataac ataaactcat ttatctctcc cgcctagcta tacttgacat gaatagaaac
     1141 agctgcctaa tctaaggaag tacataaaaa aacagtagtt agacagtaac acatgtggtc
     1201 tttgctgttt caaacacaaa aaaggcattg aacgaatacg cccaatacct tgacatagca
     1261 aataaaacaa cgtgcagacc atggaaaata tctacatagg ggtagaattt tcaccctaga
     1321 ttaggtacaa taaattaggt cgatgcactg cattgactat ggcaaaatga tcggtcaggg
     1381 tgtatgtgtg tgactggtca ttccctgatt cttagcgcca actaagggtc agggagcctt
     1441 tttttattca gctgtttcta tctgaattta ttgtaagtaa tctcttatcc aagtgcaagc
     1501 aagaaaatac aaaaaaacat tggtatgatt tatatatata taaataacat aaaaaaagga
     1561 atggcttatc ccgatacaac aagcattcct ttaaaaaaga ttcttttata taagtgaaaa
     1621 aagctttttt ccaccaatta aataaatctc tcatctcaat agtttatcca ttattttatc
     1681 tttttttgcg tatacctact aaatattacc gtcttcttct cgttcttcta ctttgttcga
     1741 gagaatctgt actagaaaaa gtagttgtat gtctaccttt tctgcctatc cgagactttc
     1801 ttttcatata tgggatataa atgattagat acagtacagc aaaaaataaa gcaaatacgc
     1861 aagcttccca actacctaac gccaattcaa ccatcaaggt agcaacccca acggatgctg
     1921 cataccagat aaaaatcaga aacgtttctt tttttctgac catgcgaaac atgataaata
     1981 gactaataag caatagtggc aacacaaata tccaattgct tccttccatc agccacttga
     2041 gtatatcaag cagcgactgt gcaacagcga ctaattttcc catagtgaac acacaccttt
     2101 ttttataatt aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat
     2161 ttctcttatg taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa
     2221 tattttctga atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg
     2281 atctagagtc aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa
     2341 taacaaatag cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag
     2401 ctttttactc aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag
     2461 tccatattca atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc
     2521 tttaaactct ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag
     2581 ctgtatctcg tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt
     2641 cagtagataa cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc
     2701 tacaaaccct tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc
     2761 gtatttagat gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc
     2821 ttcataatcc atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc
     2881 cagcatgacg acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc
     2941 cagtcctctt ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac
     3001 taaagcgagt aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc
     3061 atcaaagtga tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat
     3121 tcccgaatcc aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag
     3181 catttctttt gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg
     3241 aatgactgct tttttgacct tcacttgct
//
LOCUS       DAZULG010000182         3241 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000182 DAZULG010000000
VERSION     DAZULG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.187"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..503
                     /locus_tag="ACG9CY_002749"
     CDS             75..503
                     /locus_tag="ACG9CY_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS4939196.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACG9CY_002750"
     CDS             490..732
                     /locus_tag="ACG9CY_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4939197.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACG9CY_002751"
     CDS             complement(1032..1247)
                     /locus_tag="ACG9CY_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939198.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ACG9CY_002752"
     CDS             1354..1668
                     /locus_tag="ACG9CY_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4939199.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="ACG9CY_002753"
     CDS             1772..2950
                     /locus_tag="ACG9CY_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4939200.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT         1068 a          487 c          651 g         1035 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 g
//
LOCUS       DAZULG010000183         3224 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000183 DAZULG010000000
VERSION     DAZULG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.188"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..601
                     /locus_tag="ACG9CY_002754"
     CDS             <1..601
                     /locus_tag="ACG9CY_002754"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4939201.1"
                     /translation="TIIIPNPIKENLPYYNKTTSSKKFITACRLDKQKNLPLMIDAIG
                     KLIHEGYECELDIYGEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVIS
                     SDYEGISNSMLEALAIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRR
                     VLLDRESVVRKAQKGIKLRKELTSKNISNIWYEYLNQFF"
     gene            620..2044
                     /gene="wzy"
                     /locus_tag="ACG9CY_002755"
     CDS             620..2044
                     /gene="wzy"
                     /locus_tag="ACG9CY_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFS4939202.1"
                     /translation="MKEKINILMFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAIC
                     TWHWRKGDSIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSES
                     DMIMVKWYSCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIV
                     VPIVFVSKLLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKV
                     ITRFVFIIFALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIG
                     IYILNVITKARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNG
                     IYPFANTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNG
                     GYIGGIVYIFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIV
                     KNFVYGVLIILLINFFLQQFNKRL"
     gene            2090..>3224
                     /locus_tag="ACG9CY_002756"
     CDS             2090..>3224
                     /locus_tag="ACG9CY_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HFS4939203.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIA"
BASE COUNT         1081 a          414 c          585 g         1144 t
ORIGIN      
        1 aacaattata attcccaacc caataaagga aaatttacct tattataata aaactacaag
       61 tagtaaaaaa tttattactg catgtagatt ggataaacaa aaaaatttgc ccttaatgat
      121 tgatgcaata ggaaaactta ttcatgaagg atatgagtgt gaactggata tttatggaga
      181 agggggatta aaaaccgagc ttgaaagtta tatcaaccat aatggattaa cagaatttgt
      241 tcatctaaaa gaatttacaa ataaaataca tgaaaagatg caaaatgcgg caggatttgt
      301 aatttcttct gattatgaag gtatatctaa ttctatgtta gaagcactgg ctattggagt
      361 gccagttatc gctacagatt gtccagtagg tggcgcaaga atgtatttag gtgatagaaa
      421 tgaaataggt catcttgttg aatgtaataa tctagataag ttaacagatg caatgcgtag
      481 ggtacttttg gatagagaaa gtgtggtaag aaaagcgcaa aaaggaatta aactaagaaa
      541 agaactgaca tctaaaaata tttcaaatat atggtacgaa tatttaaacc agttctttta
      601 accaaaagga ggatattgtg tgaaagaaaa aataaatata ttgatgtttt cgttaacgtt
      661 agtcgcgttt acaatatttt gcgctttttc attaaatcag aactattggc taaattggct
      721 tggaatattg atttacttat atgcaatctg tacatggcat tggagaaaag gagattcaat
      781 attttcatta tacacgattt ttttcactct ttttttatta tttagttatg ggcaatgcat
      841 tatgtgggca ttcggtattg gaacagatcg tggaatcggt acaacagtag tagcttatgg
      901 tacggggatt attcctagtg aatcagatat gataatggta aaatggtatt cttgtatttc
      961 tatgtttgta tttcacatag gagcactctt atttacgaga aaaaccacac taagaaaagt
     1021 tagttggtat gaatctggag atgctgatgc cgatagaaag tttttattta aaatagggtg
     1081 tatcatttct ataatagtag taccgatagt ttttgtttct aaattactag aggtacgtat
     1141 agcattagtc catggttata atgcattata ttatggtgat tatgctacac aaagtggata
     1201 tttacaaatt attctatatc ttttttttcc agccttaata tgtttattaa ttggtagcaa
     1261 cttttcaaaa gtaataacga gatttgtttt tattattttt gctttatatt ctttattagg
     1321 aattatgtca ggagacagag gaagttggtt gtatagctct attatactca tatgggcata
     1381 cactcaatat aaaggaacct ttaattataa aaaattaata aagtggctta ttttagctgt
     1441 tataggaata tatattctaa acgtaataac gaaagcacgt gatggtggag gactaagtaa
     1501 acttacttta aaagatttta catctgtatt tgattcagat gattctcctt tagttgattc
     1561 tttttttgaa atggggggga ctatgagtat tataactttt ttcctaataa gtggtaatgg
     1621 aatatatccc tttgctaata cttatttgac ctcactctta ggctctatat caacaagaat
     1681 gctatcaatg tttggaataa agtttgtttt attagctgat tggttttctc aggattattt
     1741 aggaatcact tgggggactg gattctcaat gattgcagaa gcctatgtca atggtggata
     1801 cattgggggt atagtctata tttttattat tggtatagtc atcggtaaaa tcctttctaa
     1861 tagtatagac aaccaagata ttgaaaatca tcctatgaag atgtttttgg ctataactac
     1921 agcaaatatt ataatgggtt ttcctagagc tgcttcatat ttaattgtga aaaattttgt
     1981 gtatggtgtt ttaattatat tgttgataaa ctttttccta cagcaattta ataaacgtct
     2041 ttaatattta agcttaataa aaagataaaa caagtcagga gttgcaaaaa tggaaagaac
     2101 tgctcggctc aaaaaaaaca ctatttgggc tctggtgttt caaataactt ctattgtctg
     2161 tggtatgatc ttgctacgtt tgatattaac aaagtacgga tccgaggtta atggaatgat
     2221 tagctctatt aaccaatttc tacaaattat ttctttttta caattaggag taggcgctgt
     2281 aatgcaatca actctataca aaccactagc agaaaaggat aatttaatga taagtcaaat
     2341 cttagcttct ggagggaagt tttatcggca aattgcatat attctactag tatacgtggg
     2401 tttgttactt gcagtctatt atgttctcgt tcctaatgaa tttgggagat catatacaat
     2461 ggcaatgatt gcaacattaa gtattaattc atttgctcaa tattattttg gtattgttaa
     2521 tactttgtta ttaagtgctg accagaaaat ttatattacg tatattgcac agacaatagc
     2581 atttatttct aatacagttg catgtgcttt attaattagt ctgaacacag agatccttat
     2641 ggtaaaactt attactgcta taatttatct tgcttgtcca atatattata gatggtatgt
     2701 ggatcgaaac tataatataa agaggaatgt gtcatatgat aaggaaccga tccctcaaaa
     2761 atggaatggg gttgcacaac acgtagcttc tgttgtacta gatagtactg atgttgtagt
     2821 gttatctata ttttctaacc tgatatatgt atctatatat aatgtgtaca atttagtaat
     2881 atacggagta aaacagctat tcatggctat gacaaatggc gttcaggcct tactagggga
     2941 actttgggtc agaaaagaaa tagaagttct ttacaaaaca tttgcatggt tcgaatggat
     3001 tgtacatact ttgacagttt ttatttttgg ctgtaccggt atactaatcg ttccatttat
     3061 tcaagtatat acaagaggag taacagatgc taattataat caattatcat tcgcagcttt
     3121 acttgtaact gctaacgcac ttcactgtct gcgtacacct tatcatgtaa tgataaaagc
     3181 agctggtaaa tataaagaaa ctcaaaatag ttttataatt gctt
//
LOCUS       DAZULG010000184         3199 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000184 DAZULG010000000
VERSION     DAZULG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.189"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG9CY_002757"
     CDS             301..585
                     /locus_tag="ACG9CY_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939204.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG9CY_002758"
     CDS             588..1004
                     /locus_tag="ACG9CY_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939205.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG9CY_002759"
     CDS             complement(1141..1860)
                     /locus_tag="ACG9CY_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4939206.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACG9CY_002760"
     CDS             2522..2608
                     /locus_tag="ACG9CY_002760"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4939207.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..>3199)
                     /locus_tag="ACG9CY_002761"
     CDS             complement(2910..>3199)
                     /locus_tag="ACG9CY_002761"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939208.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAZULG010000185         3125 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000185 DAZULG010000000
VERSION     DAZULG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.190"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..343
                     /locus_tag="ACG9CY_002762"
                     /pseudo
     CDS             68..343
                     /locus_tag="ACG9CY_002762"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            370..669
                     /locus_tag="ACG9CY_002763"
     CDS             370..669
                     /locus_tag="ACG9CY_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFS4939209.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            843..1394
                     /locus_tag="ACG9CY_002764"
     CDS             843..1394
                     /locus_tag="ACG9CY_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFS4939210.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1540..1833
                     /locus_tag="ACG9CY_002765"
     CDS             1540..1833
                     /locus_tag="ACG9CY_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFS4939211.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1826..2122
                     /locus_tag="ACG9CY_002766"
     CDS             1826..2122
                     /locus_tag="ACG9CY_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939212.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2197..>3125
                     /locus_tag="ACG9CY_002767"
     CDS             2197..>3125
                     /locus_tag="ACG9CY_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4939213.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQ"
BASE COUNT         1164 a          436 c          637 g          888 t
ORIGIN      
        1 ttgggctatt tttatggcaa aggagggaaa agaaatgttt caaaataaag gataacgtta
       61 tgtcacaaag ggagtaatgg acagtttgcc ggtggaatta caagctttat gttggaatct
      121 aatcgatcaa aacgttcaaa agcaatttcc tttagactat cttcaaattt ttgaattttc
      181 tacggaaaaa ggaaatcaaa agttggttca tcgtcaagaa gaaccggaag aaagaaaaga
      241 gtatctgatt tcacctaagc ttcgtttgaa atcagtgtct caaaaaatat gggccattga
      301 ttcgggtgag tatcaaacga tgttattacc agaagaatac tagtatctaa aagaaaggaa
      361 cgaaaggact tgttagaaac ggctatcgaa gtactggaaa agtgtgcaca gctggtcact
      421 gcttctgaag agtggggcta tgaatccgtt acgatggaaa aagaggaaat tgaaatgggt
      481 atgcttcctg aagatgttca tctaccacgt ttagtgatga cacatcttta tatttattgc
      541 gcaccagaag atggaaaaga ctatgtagtt tatttcatta cagatattac cagccagcga
      601 gaatttgttc gtgggttact ggtagaagga cgattggttt ggagtcagat tggaggaacg
      661 gatgaataag atcgattatt tgagaatgat tgcgaaatgt ttagaagata tccttaccgc
      721 gattcatcaa attctgaaga aaaaaggaga aagaatatga aatattttca actagtattg
      781 acacttataa cggcaatggt aattgaagtg cttgaacaaa aaatagcata ggagaaaaag
      841 agatgattga aaaagaatta gtgaccacac aaacagaagt ttggcgagat cctcatcata
      901 cccatcgcta tgttttcaaa cgacaatggg cgatcaaagg aaaagaagcg gaagataaat
      961 tagcagtagt cattactatt cgtccaacag atacagaacc atttacgaat gatttgagta
     1021 tgttattaat cgaaaagaat atccgacaat taggatttac tggttttatt gctgtgaatc
     1081 ttttttcgta cacaaaagcg aaaagtcctc gggtatttct tcgaggaaat gatgaacaat
     1141 ctttggaaat attaacgact gtttttacag aaaagaaaat caataccatt atttttgctt
     1201 gtggttcgat tacagcgacg agtcaagtgg cttatgaaca agcaaagact atttacgatc
     1261 aattgagtgc gaaacaaaag aagatgacca aagtattgat aaatgcagaa ggtaagttat
     1321 cccacccact aagtattcat gttcgtaatg aatggcaatt ggcagatagt caattattat
     1381 ttcaagaaaa ataacatttt attttctcat gttacttata agtagtttta catgcttttt
     1441 gactttaaaa atacatgtga tgatacatgt tatatgaatt atcaatgtac ccagtaatat
     1501 gctattttca ttatgtaaaa aataacaggg ggtgtgtaga tggagattga tgaaacttgt
     1561 ttgagtaact cttataaaag tatgaatgac ttttttgagg aaatggaact ggattcagaa
     1621 ttgctctatc aattttatct tgcttttcga gggcaacaga tttcttttcc aatgaaaatg
     1681 tacgatcgtg agttagttag aaaaagaatc gaaaacatga tagaacagga aaaaactgta
     1741 gatatccgtc aattgacaga agtttatggt ttctctgcaa gatggattca aaaagtaatt
     1801 aaacaaaaag aaaggagacg agtacatggc taagctgatt gtattaggtg gtttaattta
     1861 cggtggatat tggttatatc aatatatgct tgcgcatccg aatatagata aaggaaaagt
     1921 aattaagaca gttggggtaa tcatagttgg actcatagct ctaactaaga aaaataatac
     1981 taatagccca aaaataatga gaacttatcg ctgtagagga tgtggaaggg aaatttctac
     2041 tagttttaat ccatctgaga gtcctcaagg aagagaccgc tgccatacgg gctatcgaca
     2101 ttattgggaa agaatcgatt aaaagattac catttagatc aattaattgt ctgatagaat
     2161 aagtacgtga gggagaaatt gaaaggggga aatataatga aaaaatggct gttattacta
     2221 ataggaatgg gaattttatt cactggttgt acatctgaga atacgaggac taaagcgact
     2281 atagaaactg atacaacaat tgagactaaa aaaagtaaaa cgcaatcaac tgaacaaact
     2341 acaacagaaa tgaagcaatc tgtggaaaca agtgcaagtt caatcactga atcttcttct
     2401 gaagaggtta agaatacttt gtgggatacg aataaagctt ccaaattaga aacttttgtc
     2461 acacaatggg gaaagacatt aggtcaagaa tataaaagct acaatcttca gaataatgta
     2521 agtctgtatg gcactccttt accacaagcc gtgattaatg gcgattggaa aatggcaatc
     2581 aatgaagcac cagttaccgt acaatggtca gaagatggaa ctggtcatgc agatttcaat
     2641 ttagtagcag tttattctga tgtagaaact ggagaatatt tagggcagca tgtttacttt
     2701 ttcggttttc aaaatggaca acctaaagtt tatttaacgc aacaaaatca aggtaatgaa
     2761 aataattatc tgtactttaa cgaaactcaa aatcaacagt taaaacaagg tttcagtgat
     2821 attgtgaatg gacaaactcc tcaaacacca gtagtagaag aatctactca acaagcaaca
     2881 actacaacga cacaatctac aagtagttca ggaatttcaa gttgggaagc tgctaaagaa
     2941 tctgtgctaa ataataaagc tttatggttg tatgattctc ctgataaaaa tgtatcaaat
     3001 actaatttaa ttagcacaaa tgaatatatg aaagatctaa tgcaagatga aaatggaagc
     3061 tactattcaa ttaatattgt aacggatgat gatggaaaca cgaccatggc tggtggaaaa
     3121 caatt
//
LOCUS       DAZULG010000186         3040 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000186 DAZULG010000000
VERSION     DAZULG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.192"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1538)
                     /locus_tag="ACG9CY_002768"
     CDS             complement(228..1538)
                     /locus_tag="ACG9CY_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS4939214.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1550..2968)
                     /locus_tag="ACG9CY_002769"
     CDS             complement(1550..2968)
                     /locus_tag="ACG9CY_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4939215.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          945 a          625 c          473 g          997 t
ORIGIN      
        1 ttgttatttg tcaagaactt ttttgaagtt tttttcgata gaatcaagct tcgcaagtgg
       61 ttctttttaa cgacttagtt atcttatcac gtctaaaaac atgtgtcaac tcttttttgg
      121 aattttcata aaatgtttca atgaagcgcg acaacgttgt ttaatatatc attaaaaaaa
      181 ataaaggtca agctaaaata ttatttaagc ttgaccttat tataatttca acgattgtca
      241 aaagtattgt ttgcagctgt gttgccagtt tctatagata aatcacagct tttgaaacat
      301 aagttttgta ctgcttcgat tcgcccgaat tcttttgcag ttatttggca atgagaaaag
      361 cagacatctt ctataggctt ttcaggaaat gctactaaat caaaagctcg ggaaggaagt
      421 gagtaacctg gagaaaggca tgcctgtacg tttttgatgt gtatgtcttt gactttcgtc
      481 atttgctttg attcaggtat ttcttcagcc actgcttgcc aactttctgg catttgagca
      541 aggtcattgc tattttttct attgtattca ttatgccagt ccattatcca agaaaaagga
      601 aattgtacat caatcatatt cagattttct accctgatat tttcgatcac tccccctctt
      661 tcttttgaag atttcattcg aataccacag tctgactgaa aaaaatcgat atcgtgaata
      721 tatacgtcac taactccagc actaacttcg ctaccaatag ttacaccgta tcctgaacgg
      781 attttacatc tagcaacctc gattcttgat gattttcgat tgactcttgc accattgcca
      841 tctctgccag acttaatagc aatacaatca tcaccacagg ccaaatcaca ttcatagaca
      901 cgcacatttg ttgaagaatc aatatcaatc ccatctgtac ttggtccatc attgttgcgg
      961 atgattactt tttctactaa tacatcgtta gaataagtga tttgcaagtt ccaaaaacct
     1021 gatttctgaa aaatcaaatc acgaacaaca acatgttctg catgatacag taaacaagcg
     1081 cgtggacgtt taatagcata atccgcaatc caacgaagtc ccttttgatc gtattcagct
     1141 cttgtcccgc ttttttgatc ttttccccaa tacagctccc accaatggtc tccttgaccg
     1201 tcgattattc cttttccttc tataagaata tctttcgccg agagaacatt caaaatcgct
     1261 gctggccatt tcatttctac gccggctact cgtgtatcga tttctggaaa tgcttcgata
     1321 tctgttctcc ccttgaggac tgcaccttct tcaaagataa gatgactctt atcttttaaa
     1381 aaaagggctc cgactaaata aatgcctttt ggaataaata caggtaagtg ttctttcgca
     1441 ccttgatcga ttgctttttg cagtattatt gtatcatctt gttgagtgtc gtttgtttta
     1501 aagttcaaag cattgataat cccttttttt gtccgcaata cgtttctccc tatctttcat
     1561 tgattttttt caattcttca ccaataaata cagtttttga acgattcagt gggtaaccta
     1621 ctagcaaaat gattgcggat acaaataagc agaatgctgg taacaagttt gccatcgtat
     1681 aaatcctttc ttgtactaaa actgattgga tggctccacc tgttgtagac gattggtaac
     1741 caatgagtgc cagcataaat ccgcctaccg ctcctgcaca tgcttgtcct acttttctcg
     1801 caaaagaatt tactccatat actgttccat cttctcttaa gcctgtctta tattgatgat
     1861 aatcaatcac gtcagtaatg aatgcccaga ccatcaaatt gaataaacca gatcctaaag
     1921 tagcgataaa taaaagaatc agatataccc atgcatttgt tatatgaagg aaatacatca
     1981 gcaagtacat acatgaagaa gcaagtaacg caaatacgct tgcttccttt ttaccaaata
     2041 attttgtcag ctttgtagca aatggtgcta aaactactac tgttccgtaa gtaaataaaa
     2101 gagcaacaga cattgcttgt ttattatgga agtaatcatt gaataaataa gtcatattcg
     2161 tcccagctaa aatctgattg atcacaatga aaatgtcaac aatgactaaa ataatcagtg
     2221 ctcgattagc taacaaacct tttaccaact gagaagcaga tactttttcg tttttgacaa
     2281 ctcttactct ttcagtagtc aatttacaag ttaacatata tgcgacaaat gctattgcag
     2341 cacatccgat tgaaatccaa aagaaatgat tccctgaaac gacttgttgt ccactcttgt
     2401 tatgggtata cattaaaatc ggaatgaaga atcctgtagt tgatccgcca atcgcagaac
     2461 caatgcttcg gaacgtggac aacgaggtgc ggtcattagg atttgagcta attgctgctg
     2521 ccattgctcc atacgggata ttgattgttg agagaaaaat cccataaaca atgtatgaaa
     2581 caaaaatata agcaatcttc accgtcattg gaagatgttg gacgaatggc aaaaacaaaa
     2641 tgacagtcac taagcaaaat ggatacttca tttttctgat ccaaggaata aatcggccag
     2701 tttctgttaa ctctgagaca tcacataatc ttccaaccat cacatcagca aacgcatcca
     2761 aaattctcga aatcaagaat aacgtaccta cgattgcccc tgaaacgcca agaacattcg
     2821 tataataaat catcaagaaa ctattgacta aacctaaaat aaaacagtta cctagatcac
     2881 cgaacatgta gcctatctta tcggaaatcc caaattcctt tttttctttt tttattgtat
     2941 ttgctgtaac agcattttgt gaaagcattt gaattcctcc tacctcttct agtaaacgtt
     3001 ttcaaaatta taactctttt tttcttgttt ttatattcac
//
LOCUS       DAZULG010000187         3029 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000187 DAZULG010000000
VERSION     DAZULG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.193"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            875..1201
                     /locus_tag="ACG9CY_002770"
     CDS             875..1201
                     /locus_tag="ACG9CY_002770"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4939216.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1209..2117
                     /locus_tag="ACG9CY_002771"
     CDS             1209..2117
                     /locus_tag="ACG9CY_002771"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4939217.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            2743..2997
                     /locus_tag="ACG9CY_002772"
     CDS             2743..2997
                     /locus_tag="ACG9CY_002772"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFS4939218.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
BASE COUNT          949 a          615 c          553 g          912 t
ORIGIN      
        1 gtgatttatt tttattccca tacttttttc gttggtcact ttcgtatacc taatctaagc
       61 gatcatcggc gacggtgctt ttacaacgaa tggcaggaca aaacaagggg cggaaaaact
      121 tttttgccta tttcataaaa aactttttcc tacagcaatc ccttgttttg tcctaccaca
      181 cgttgtgaac ccaaagcacg ccgacgaacg cttagctata cgaaaatgac ctccaaaaac
      241 aaaaaagtga ggtgaataga aacaaaaaga aattgaaatc tcccaaaatg tcgtccgcct
      301 cggagacaac ttgctgctga aaatggttgg ttaattcatt taagtattaa ataagataaa
      361 tcatcaaatt aatcggaaaa atagcatgat tggccttatc aaaaataaac tgtaaaaact
      421 tatatttcta tgagtggtgg aacaatgcta ataccaaatt aatttatcga ttacccacct
      481 gcacaaacca aagatagcat tattaggaaa cgaatcaatc agcgaatcca gcattataat
      541 gaggatacta aaaataaagt tttcataatt ataaatccgc cttcctacga aaggcaccaa
      601 tgaggtgatg aaaaccttag agcgctttct ttcttttttt attccgtgtt accctttcct
      661 tgcaggtccc tggtacacta cagaacccga ggaggtgagt ggcggggctg tatagcggca
      721 accccgcata attatatggt gtcggtcatc cgttaaggca tcaatgaatg gcgcaaaccc
      781 gtagcccgta aacttatctc ttccgaagtt gactcgattt cgccggatga ccagtcccta
      841 ccaaacctgt actatttttt tacaggaggt tgctatgttc aaaatctttc gtaaaaattg
      901 ttgtggcttg gatgtccaca aaacgtggat ctatgcgtgt atcggaatca cagatgcgaa
      961 tggtcgtaca gtgtataagc aagctcgctt ctcttccttt acaaaaggtc tcaatgaatt
     1021 agctgaatgg cttgctaaat acaactgtaa ggatgtgtgt ttggaatctt ccggtaaatt
     1081 ttggattcct gtttttaata ttcttgaaaa aacttgtttt gtcacactgg ctcatccgaa
     1141 gtacaccaaa ccccagaaag gaaataaaac agatcgcaaa gatgcaaaaa tggatttgtg
     1201 atctctttat gtgcgacatg atcaaaccca gttttattcc tggtcttgaa atccgccagc
     1261 ttcgtgatct aatgcgttat cggacgaaac tgaccaacat gaaaacaagt gaaaagaatc
     1321 gtgctttaaa ctgcttaacc gtttctaatc tgaaactaga tgatgttttt agtgatgtgt
     1381 tcggaaaatc atccacttct atcatccaat acttgttaga acatcccggt gaatcatttg
     1441 atgttcgacc atttatccat gcccgttgta aaactcccgt tgaagaaatc caagcggcag
     1501 ttgagggggc gatttctgta cctcaagcga ttaagcttcg ccaatgcctc aaccatatta
     1561 acgagattga gaagcatcga gaagagatca atctagagat tcaagctctt gcagaaccct
     1621 tctcccctgt tttggaacta cttcgtacca taccgggctt agacactcag ccaataacag
     1681 cgattgcgat tctttcagaa ataggaccgg atatgtccgt gtttccaact tcgaaacact
     1741 ttatttcttg ggctggatgc tgtccaagaa acgatcgcag tgcccgacga acaaaatcaa
     1801 cacgtatctc tcgtgcaggg cagtcgttga aaccgctact tgtacagatt gccaatgctc
     1861 ttctcagatc caaagaacat ccagaattta aaaatcgcta tcgtagaatc aaagcgaatc
     1921 gtggacacaa gaaagctatc attgctattt gtaagatgct cctgaccgct atctggaatg
     1981 tcttatcaaa ggtggaaccc tattctgcta aaggatatct ggagccaaaa cctgcgaaag
     2041 aagagaaagt tctgacgcaa tcccaagctc tggacctttt aagaaaacgc ggatatacca
     2101 ttatagatag tgcttaaacc aaagttacgt tgattcttcc taaatagact cgttgtcttc
     2161 catttcaaaa attttgaaaa cggtaggctt atttagtgta ccgttcttca ggtatcttca
     2221 aatagattta ttttcaaact tatttccttt cttcttttac tctcctatag cttagcattt
     2281 aagtgggcac tcgtgtcacc ttgctagata gctatgaatg ccgtaataag cctcctagta
     2341 tataggcaga ctgccaatta taactgattc aatccacatt atgataaaat ccaccttttc
     2401 gtttcatatg tgttgaaata tcacatatgt caaaagtgca cgagtctttt cattcagtgc
     2461 tttgtaaata ctcaaaatta ttccttttca ctttaaaaaa aagataatta gtacaaaatc
     2521 agaaatgagg aaagtattag gatggactag aaaggagttg ttgaatttgt tgcaacagat
     2581 tggtcattta tcaattaaaa attaggtgaa gttttattca tcactgcagc tgctgtttcc
     2641 cagagaataa aacgtctaga agaaactggt cagttactgt attatcaagc agcaattgac
     2701 tacgaacaag cttgattact aaccaaagcg tttatccacc tgttgattaa atcaaatcaa
     2761 aaatcaggtt tttatacgtt cataaaagca atctccagtg tactcgaatg tgactgtatc
     2821 ataggtcctt attcgatgtt aatcaaaatg gggtttcctt ctaccgaatc attggacagt
     2881 tttatcaatg atttatcaaa gtttggacga accaacaccc aaatcgtttt ctcgactctt
     2941 gtttccttaa tggtttatca gttctggaat gtttatcatg cgcttttatt aaaatagaaa
     3001 agagcttttt tcatgcgaca aagcagtga
//
LOCUS       DAZULG010000188         2948 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000188 DAZULG010000000
VERSION     DAZULG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.194"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..456
                     /locus_tag="ACG9CY_002773"
     CDS             175..456
                     /locus_tag="ACG9CY_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939219.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            458..874
                     /locus_tag="ACG9CY_002774"
     CDS             458..874
                     /locus_tag="ACG9CY_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939220.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(1211..1351)
                     /locus_tag="ACG9CY_002775"
     CDS             complement(1211..1351)
                     /locus_tag="ACG9CY_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFS4939221.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAV
                     VN"
     gene            complement(1348..2322)
                     /locus_tag="ACG9CY_002776"
     CDS             complement(1348..2322)
                     /locus_tag="ACG9CY_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4939222.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          994 a          603 c          438 g          913 t
ORIGIN      
        1 aaaaaaaagt gggtagatag gctgtgccta tcacgtttct cagaaaaaaa tttgatatca
       61 ctttttcgcc atcgtggcta gcttatgtat agtattttag caaaaaacag ctatacaaat
      121 actatattcg tatgatatac ataaattata cacaaatgcg aaaggagttt ttctatggac
      181 attttagttc ggaagatcga ttcaaaggtt attgcaaaaa tagacaaacg ttgccaagaa
      241 ctcacaaata aaactggcaa aaaatggagt cgaaatcgct acttaaaagt cctaatcgaa
      301 aacgattttg accacgcact tatgcattac aagaaagatc aattcgattt attactcgaa
      361 aaatttatcg ctattcaaga aaaaaatact gaaacgctga atgaatatgt tcgtacaaac
      421 aaccaactca ttgaaacact tatcggagga agataacatg agaaaaactt ctgttcaaaa
      481 acacgttacc ctttatccca gtcacgtgga cgttttagat tccattatcg aaagaaatcc
      541 agatatcaac aattactcgg aagccattcg ttttttatgt atgagttatg aacctgatac
      601 caacacgact actaccagca atcaagccaa actaaatgct atgggaaaag aaatttccat
      661 catgtccgaa atcattgcag aatttgctga tttatatcta aaagatacag gtattcttat
      721 tggcacaaat tctaaagtgt atcaagaaac gaaagaaaaa attgaaaaac aacttaatcc
      781 ctccaaacga aatcaaccaa aaaatcgtca aaaagaaacc aatcaaacat ttacttctaa
      841 aaatttcgac ccaacaaatt ttctaaaaag ataaaaaata aaccaggaga tttaaattcc
      901 tggtttattt tttgggtagg atcaccatct taatagcaac atttacaata agatagtaac
      961 caattgaatg aaaaaaacga atttcagaag aaataacaga aaaaaagata aaaacaaaaa
     1021 aattataaat acatgatttg aattcataat cgtaaaaaaa gctgctcgta cgagcagcta
     1081 aataagaaaa agattggtat gttttaagga taaaataagt ataacaaatt tttcacatag
     1141 agcaaagggg gcgaaaatcg cccccttttt ttcgttttaa aaccccaaaa cccaaagaca
     1201 taaatttcgg tcaattgacc actgcatttt tttctttata ctgagcaagg tcaaatcccg
     1261 aattgaccag ttgattgcgc accgtatttt tcgatacacc caattcatca gcaagttttt
     1321 ttagggtcat tttttctatc gaatcactca ttcagcacct acttccgtca catcaagtcc
     1381 ttgaagccat gtatctaatt ctcgtttcaa atcatctgcc gactccgaaa atgtttctcg
     1441 caaatgattc gtcaactcat cattttgact ttcttgcttt aacaattcac gatatttata
     1501 ttttgaattt ctatgaattt gcccccagta actatgttcc gttataagag cagtaatcat
     1561 cattctgtca gttactttca catcttctgg caaatagtga ggacttgcaa agctatctaa
     1621 actttcatga accattgcat gccaatcagt tgctttcccc cttcgcaatt gcaattctag
     1681 tcgccaccaa gtatcgattt ctttcggaac aactttccgt tttctttctt gttccaattt
     1741 tttgttatac atccgaatct gtcgttcact agcacgactt ccccaatatg ctgtttccag
     1801 tttcccagac cgaccgtaaa ttggtttgaa agaaacagga tcaaccaccc gatattgggt
     1861 aatgaaatca tctggcacgt caataatgtc acaagcaata tctgcacgtg agaaatgtgg
     1921 ttctaaaaat aaatcatgga taaagttctt catcgagccc gccagaaatt ggctaatttt
     1981 attggggtta aagtcaatcc gcccttttcc ctcttgaaac ttaagaatct caatataagc
     2041 tatattttcc tcatgttggt ctagtaattg ccagccatta tttccaaccg cttttaaata
     2101 tccgttacct tcgtttagac gcataagctg ttcaaaatct aaaatcaaca catcatgggg
     2161 agtgtgataa taaatatttt cccttaatgt accaacaatg gtaattcggt caattgacca
     2221 ttgcattttt aagggggtgc acatagtttg tgcatttaac cgcctgttag atatgcggtt
     2281 aagtttttcc cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa
     2341 gaaaaaagag atttttggaa agaatttcca gagttcacgt aactctaaaa atccatatcc
     2401 aaaaatctct agcttgaaga aacctaaaaa tattcgtata atgaatttga gttacggaac
     2461 taggtgagta gtttccgtaa ttagccgaag ggtgcttcca acaccgttcg gcttttattt
     2521 tgctcttttt tctcgttttc tttgattata gcactcctct taaaaaaata aaaagacctc
     2581 agggaatttc attccccgaa ccccttgagc ctcctcctgc cgaaattatt tcacgcttcc
     2641 gctcttcatt tcattcagtt ctcggcttgc acaatctaac acgccgtttc gctaccagtc
     2701 gccttacagg ctccattctc gcttctctta cgtgttttaa cgattgtgcc gataactaaa
     2761 cgaaatgaac gctaaaacgt cttagaacgc aaaatacgcg cttataaaat catttcatct
     2821 tgattacatg cccgtcataa tcgttcagtt tagtttcatt ttcgtaaact ttttttattt
     2881 ttacgaacag aaaaaagtcg cacatcacca ttttataaag agcataagga aacgtgtacc
     2941 cactttct
//
LOCUS       DAZULG010000189         2932 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000189 DAZULG010000000
VERSION     DAZULG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.195"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..475)
                     /locus_tag="ACG9CY_002777"
     CDS             complement(113..475)
                     /locus_tag="ACG9CY_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4939223.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            698..1450
                     /locus_tag="ACG9CY_002778"
     CDS             698..1450
                     /locus_tag="ACG9CY_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4939224.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1590..2513)
                     /locus_tag="ACG9CY_002779"
     CDS             complement(1590..2513)
                     /locus_tag="ACG9CY_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4939225.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2528..2800)
                     /locus_tag="ACG9CY_002780"
     CDS             complement(2528..2800)
                     /locus_tag="ACG9CY_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939226.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT          945 a          566 c          482 g          939 t
ORIGIN      
        1 ctgtcaatag aatggaaaca aaaatggcga gacatcaatc agtctcgcct tgttttttat
       61 tgtatacatt agatatggag caggttacca taaagatgat tattagtcgt tattaaatag
      121 aatccttcgt atttttttgt tctatcacta caatatcttt atttggacgt ttgaattgta
      181 ttccggcacg ttctagctcc tgtttcactc ctacatggac tttgacatct tctcgtgtca
      241 tttttaaaac gtgatgaatc gcatagtcga tttcttgcct tgtgattttt ttcttcgtat
      301 tgatcatcac gacttgcttt ttatcatatg tgtctaccaa tagcagttgt gttcgatcta
      361 acgaaaataa tttgtaataa ttaatagccg tattcccaaa aaagaattcc actactttag
      421 gataaaatgt cttaatattt aaattactat tcacaggtgt ttcgattagc ttcatctcat
      481 tcctcttttc tatatagtcg atttagatcc aatcttaagt tatgttacca tctttcctag
      541 cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa ggatttacct tttatcactt
      601 aatagtataa cataatttca tgagaaaaaa atacaatttt ttcttgacca tctcgttacg
      661 taaaggatta tgcttacatt tgtcaggagg aaaaataatg gagtacacaa taaagaaaat
      721 gtcagagata tcaggagtca gtccgagaac cttgcgcttt tatgatgaga ttggactgct
      781 aaaaccagct aggatcaatt cttcaggtta tcggatttat ggcaaaaaag aagtcgatcg
      841 tctgcaacac atcttatttt atcgaacaat ggcattcaag ttggatgata tccaagaggt
      901 cttagacaat ccatcttttg accaccagaa agctttaatc aagcatcagc aaatgttgct
      961 ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc caacagacac ttgatatgta
     1021 cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa gggtttaaac aacaaaagtt
     1081 gaaagaaaat gaagaatcct atggacaaga gattcgacaa aaatacggaa aaaaaaaggt
     1141 tacagcatcc aatgaaaaat ggatgaatat ggataaagaa acgtatgaag aaatgcaaac
     1201 tgttgaaaag cagatgttat ttgatctcac tctttactta aaagaaccag aagatgaaag
     1261 cttagcagac cgtatcttcc aagctcataa gaagtggtta tcttacagct ggcctgaata
     1321 tcagccaaaa gcgcataaag gactaggaat gatgtacgaa gccgatgaac ggttcactgc
     1381 atattatgac gagcgaagtg gtcatggtgc agcaaaagct ttaaatgaaa ttatccaaaa
     1441 aaaggcgtaa tcttttacat gatccaacaa aaaagtacac gtaaaatgac ctaataacaa
     1501 aacagcagac acccaagtta gatttttatg ctaacttttg gtgtctgctc cattctttca
     1561 caagtttttt catcctactt taaatatatt catcgttgaa taaacgtata aggtattttc
     1621 actttatcct ttttttctgt aagaaacaat tgaaatgcca tttctccgca agcttccaga
     1681 tggtggtcga tggttgggaa accaagaagc tcgctgatca gcaaattatc gcgtccaatc
     1741 accactggtg tcttatcttg cagatagctt tgcaagatgc ctgctgcaac catatccgaa
     1801 tttgtaaaga caccatcaac tccaagctct ttaaaaaaga agcctgcttg ttttcctttc
     1861 tctgcatcta tacaatccca aaaaacaagt gattcatcaa aagatggaaa aagttgatga
     1921 catagctgca aagtgatttt tgagttataa cttagcctgc cacttcgccc caacgtgatg
     1981 cccaattgct ttataccttg ctgcttcaaa taagtaagac cttctgtaat tgattgttct
     2041 cgatcaataa agacacagct tgcctgtttt cccttgattt cttcacaaaa aactagaggt
     2101 ccatattttt gatatgccaa taaatcatct agtgtattgg cacgagacgt aatgatcaat
     2161 ccatcgaaag ctttagctgc aaattcttca agataccttc gctctttttc tttctgatag
     2221 cccgtcggta aaagtgtcac tttgtaatca taatcaaacg ctgcttcaat gatcccactg
     2281 atcaactgat caaaatacgg atgattggta taaggcagaa tcactccaat gcttttcgtt
     2341 tttccataac ttaactgtct ggcatttgca ttagggatat aatcgacttc tttcatgacc
     2401 gcaagtattt ctttcctttt ttcctcatct acatatggat gtccattgat tacacgtgaa
     2461 atagtagaaa ctgaataacc tgtgagcttt gcgatatctc gaatgtttgc cactgctgct
     2521 tcgccaccta tacaaaaaga ttttcttgta aatagtctac cacagctgga ccgcattcaa
     2581 aaaactgata catttttcct tcatgcgaaa aactgatccg ctgcccgccc caatatatat
     2641 cttttgtcaa attttcgatt tcattcattg gtatttcttt tactatattc ctattcaacc
     2701 cttgaaaact gtacaacgtt aatacggatc ccttgaactc gacaaacgca taaaaataat
     2761 tgttcgttgt gccatctgtt atctctacat aagtattcat tgcaagccct ccttttgata
     2821 ctttgagtat agtgtatgaa acgcgtctca tagcaaggag tttgttttgc caaagaaaag
     2881 ttttattcct ctttcattat taccaaagtc tcttatttta aattgaatac at
//
LOCUS       DAZULG010000190         2889 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000190 DAZULG010000000
VERSION     DAZULG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.196"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            776..1162
                     /locus_tag="ACG9CY_002781"
     CDS             776..1162
                     /locus_tag="ACG9CY_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4939227.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1260..1451
                     /locus_tag="ACG9CY_002782"
     CDS             1260..1451
                     /locus_tag="ACG9CY_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4939228.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1965..2297
                     /locus_tag="ACG9CY_002783"
     CDS             1965..2297
                     /locus_tag="ACG9CY_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4939229.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2207..2401
                     /locus_tag="ACG9CY_002784"
     CDS             2207..2401
                     /locus_tag="ACG9CY_002784"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4939230.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            2440..2586
                     /locus_tag="ACG9CY_002785"
     CDS             2440..2586
                     /locus_tag="ACG9CY_002785"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939231.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
BASE COUNT         1060 a          475 c          447 g          907 t
ORIGIN      
        1 tgaattcgga attaaaaacc acgataggta actttaaaca aacatgaaga gactttaata
       61 agacttcttt atctgaaaac acttttgttt ctctctttcc tttctacatc attaaatagt
      121 attttatacg ttatctaaaa tactattcct ctgtcaaaat aagctaactt acttcattat
      181 aaaatatcca cttatcgctt tcatgtatat tccaccgtaa aaagtacatt ttatcaccga
      241 gaactcatca tttttcatca tgatgaatac tatttacata tatctagttc aaagacaata
      301 attattatga tccgttatta tctaagcact atacagataa taggaatttt tcgtttctaa
      361 atactccaat ttgggaatcc aataaaaatt ttcgttcaaa tacgtgtcta ttcgaactct
      421 tttctagttg ttaattatga agggggaaga aataaaaaat acaatctata cacgaataag
      481 aatattatcc aaaaggaggt tttagaaaag atttattata cgtaaaaagc tcaagacatt
      541 ttcttagcca taaggacatg tttgatattt aagaagatat atgaattttc ttttctaagt
      601 ataaagataa ctatccatta tcaactaaaa aaacattttt gaaaataaac atcagcagtc
      661 ctatcaatgc atgtgagctt atagaagtta ttcgcagcgc agcataattg cattagcttc
      721 ttttcgtgtt ttttgcctct tttcccttta aatcttaatg gaggaggtgt gcaatatgtc
      781 agataacgac gaacgcgtaa atgcccagtt gattcgattc tttgaaaagg tcattttgaa
      841 caccgctaat acttattata aaaaacaaga agaaattcaa aaacatgaac aacttgatca
      901 actcccacca tatttgttta ctaaagatac tataaaattt aaacaaccga ttaatgtttt
      961 aaatgtgact ctccttgttg aagatataaa attagcaaaa atccttcctt tgttgaaaga
     1021 acctgaacaa atttttctta tcgaaaaatt tatctttaat aaaactgata aagaaattgg
     1081 aaaggttctg ggtattacaa gacagggtgc gacaaattta aaacatcgac tttacaaaaa
     1141 aattcaaaaa atgctggttt aatatctgca taataactaa ttttgtatgc ttctttgctc
     1201 gtcctcctcc ttccccaaaa aataaataga ttataagttc atgggaagaa ggtaaattta
     1261 tgaatacttt aaaagaagat ttcattttag caaagaatgg aaatgaagaa gcagttgtgg
     1321 ccattctcaa aagatttagc gctttggtcc ataagcaatc ctggagaact ggaaaatatg
     1381 atcaagattg ttatcaagaa tgtatgcttg caatatatct agctatctcg aaattcgaaa
     1441 ttaaagaata actcaactaa gggagtctgt gacataagta aaaagatagt ttcaaatttc
     1501 gaacaataaa tttgaagcca tcttctttat aattataaag tatcgatttt acagcgttct
     1561 ataatgattc atagaatcta aaacgaatga ttttaattat taacgttttt taattcgttt
     1621 ttagcttaaa aataactttt gtcacagtct cactcctaaa attactttat ttacgtacga
     1681 agtgggcagg aggtcgaatc cagaactggg ccatggtact tcaacgagga cttctcctct
     1741 agaattgaaa agcatgcgat ttaccttccg taacgacggt agatttattc aactatgtgt
     1801 ccaatcaaaa tttgtctgat ttcaaaaata gtcaagagaa ttttggttgt taaaaactct
     1861 tatctatcaa cttccacagt ttagttgaca aaccctgata ctaagttggg ctatgataca
     1921 cttttcttga ctatcagtta cgttaccgga aggaatgaac tctattgaaa aaaacatatg
     1981 atcgtaaatt taaactaaag atttcacagg atatccttga gaaaaaagta actaccaaga
     2041 aaattgctga agaatacaat atctctcgtc caacaatttc ccgatgggtt tcagaatatc
     2101 gtcgatacgg gaaaaatgct tttgctggac aaggaaaaag attacctgat aaagcagatt
     2161 tctacattct tgaacaggaa aataaacgac taaaagagga agacgagtgt tctacgaaca
     2221 caaagatcga tacggatctc cagggatcac tcaaaaactt tacgatgaag gaatcgaaat
     2281 caataaacgt gttgtagctg ttcttatgcg tgaaatgaat ttatgcacaa gaggatttca
     2341 tcgacgcaaa tcttctcatg gtagagaaaa aacaatcaaa gaaatggtaa aaaaatgttg
     2401 aatcgataat ttgaacagaa ccaaattgat gctgtttggg tgatggatat tacttacatt
     2461 ccatgtaatg acggtcgcct ctatttgtcc acctatattg acttagcaac tcgtattcta
     2521 cgttactata tggtagattc acacatgaaa aaagagattg tcgtccgtcc attacaaatt
     2581 tattaaggag aattgcctac ggtaattcat tccgatagag gtagccagca tcggtctttt
     2641 atttatcaag agctgttgaa cgaccaccac atcacacatt cagtgagcca aactgggact
     2701 cctgttgaca atgcagtaat tgaagcgttt catcgatcaa tcaaatgaga attaatcgac
     2761 tccaacaaat ataaaagtaa aatagagatg aaggtgttaa tccaagatta tttagaagac
     2821 tactacccaa acaaaagaat ccatacaaaa tttatgatga ctccttgaca atttgaagcg
     2881 aagaaaata
//
LOCUS       DAZULG010000191         2773 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000191 DAZULG010000000
VERSION     DAZULG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.197"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..562
                     /locus_tag="ACG9CY_002786"
     CDS             116..562
                     /locus_tag="ACG9CY_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4939232.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            835..2187
                     /locus_tag="ACG9CY_002787"
     CDS             835..2187
                     /locus_tag="ACG9CY_002787"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS4939233.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2358..2645
                     /locus_tag="ACG9CY_002788"
     CDS             2358..2645
                     /locus_tag="ACG9CY_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4939234.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          975 a          453 c          546 g          799 t
ORIGIN      
        1 ttttacgtct tttttcatta aaatacaata aaattgaatt ttcttttcat gttaagtgga
       61 ttttaaaatg taaaaatagt atgataagac gagaataaca acgtggggtg tttacatgga
      121 aaataagtta gtcgaagagg ttatcgaaaa gttaaaaaaa gagaacattc gaatcacacc
      181 tcaacggtac gcggttttag aatatttgat tgaacatcat tcccatccaa cggctgatga
      241 gatttataaa gcacttgaac atcggtttcc aaatatgagt gttgcgactg tttacaataa
      301 ccttcgctta tttacttcga ttggatttgt tcaagaaatg aagtacggcg atgcttctag
      361 ccgatttgat ttcagttcaa agcgtcatta tcatgtaatt tgtgaaaact gcggtaagat
      421 tgttgatttt cattacccag ggcttgaaga tatagaaatg gctgcaagca gactgactga
      481 ttttgagatt aatgaacatc ggttggaatt gtatggactc tgtccggatt gtaaagagtc
      541 agcaaaaaag gcgcaaatgt aaaaacagtt tctacaagtg ttatacctaa aaaaagctag
      601 ctgtgtaaaa aaaacgctag cttttttatt tttttgtctt ttattcacaa tcttagatat
      661 aaaagtgaat attattaaaa taagaatctt gtagatattg ggttcttatg cttgaaaagg
      721 aatgaaacta caaacaaacc tttgtgaatt cacaagcatt aaactgttgt atccgtttta
      781 tttctgttat agtagttttg tcgaaggaca aaatgttaat cggagggacc ataaatgaaa
      841 gtagtagtaa ttggctgtac tcatgcaggt actgcagctg taaaaagtat tttaaaaaat
      901 catccagaag cagaagtgac agtatatgaa agaaatgaca atatttcttt cttatcttgc
      961 ggtatcgcac tttatgtcgg cggagtagta aaggacccag caggcttgtt ctactcaaat
     1021 cctgaagaat tatcttcatt aggagcaaca gttaaaatgg aacacgatgt aacagacgtc
     1081 gatacagaaa ataaaacagt aacggcaaaa gatctgaaaa caggagaaac agaaacggtt
     1141 tcttatgata aattagtgat gacaactggt tcatggccta ttatcccacc gattagtgga
     1201 atcgatgcga aaaatgtact cctatgcaaa aactacaatc aagcaaatga aattattgca
     1261 caagcgaaag aagcaaaacg tgtagtaatt gttggtggtg gatatatcgg tattgaatta
     1321 gtagaagctt ttgtcgaatc tggaaaacaa gtgacattga ttgatggatt agatcgcatc
     1381 ttgaataaat atctagataa accattcaca gatatcttag aaaaagaatt gacagaccgc
     1441 ggtgttactc ttgcgctagg cgaaaatgtt actgaattta tcacagatga agaaggaaaa
     1501 gtgaaacaag tagctacacc aacagataca tttgatacag atatggtcat tttatgtgtg
     1561 ggcttccgtc caaatacaaa actgatcgaa ggaaaagtag atacactgcc aaacggggca
     1621 atcaaagtga atgaatacat gcagacaagt aatccagaca tttttgcagc tggagactct
     1681 gcagtagtca actacaatcc aagtggcacg caaaactata ttcctttggc aacaaatgcc
     1741 gttcgtcaag gtttgcttgt gggaaataac ttgacagatc ataaaatggc gtatcgtggt
     1801 actcaaggaa cttcaggact ttacctattt ggctggacga tcggttcgac aggtgtgacc
     1861 aaagaaagcg caactttgaa cgatctagaa gtccaagcga ctgtatttga agataattat
     1921 cgtccagagt tcatgccaac tactgaaaaa gtaatgatgg agctagtcta tgaaaaagga
     1981 acgaatcgta tcgtaggtgc tcagttcatg tccaaatacg acattacaca atcagcaaat
     2041 accatgtctt tagctgttca aaataaaatg actgtagaag atctcgcact ttcagatttc
     2101 ttcttccaac cacattttga ccgcccatgg aattacttga acctacttgc acaagcagcg
     2161 ctaggcgaaa tcgaaactgc acaataaaga aaaagggttg tgacacaatt ttgtcacgac
     2221 cctctgtctt tttttgtgaa aagtacaatt agttgtcttt cttcgaattt tttgtcacaa
     2281 taagcagcaa agataaagct gttacataaa agaacaagca gctttatttg attttgaaaa
     2341 gaaaggtgag aaagaagatg aataaagaat attttgtcca acaagatgca tttttagaag
     2401 attataaaag tgcttcgaag aaattaacga tcaatattga tgagttggca gatcaagtac
     2461 tctctcagtt gaatgaaacg caagaggaat attacacgat gccagcaaat gacgccaaag
     2521 ataatgtttc tcatcgtttt cctttcagta gacgcatcta ctcagatgaa aatgacggca
     2581 cattgaatac agagtacaca tatgaaggtc agccatatgt gctagaaact gatgatgatc
     2641 tttaaagaca gtttttgaaa aggccgctca tccttaagtg atgacaaaaa atcatcacaa
     2701 ttaccgatga ctgtctcttt actatctttt aatcagaaaa aggggttatg acaagctttg
     2761 tcaacccctt ttt
//
LOCUS       DAZULG010000192         2732 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000192 DAZULG010000000
VERSION     DAZULG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.199"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..514
                     /locus_tag="ACG9CY_002789"
     CDS             <1..514
                     /locus_tag="ACG9CY_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939235.1"
                     /translation="MTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGMD
                     YFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVY
                     AYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLC
                     LFFKIVFHKR"
     gene            689..1522
                     /gene="msrA"
                     /locus_tag="ACG9CY_002790"
     CDS             689..1522
                     /gene="msrA"
                     /locus_tag="ACG9CY_002790"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4939236.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1602..2591
                     /locus_tag="ACG9CY_002791"
     CDS             1602..2591
                     /locus_tag="ACG9CY_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4939237.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT          999 a          434 c          529 g          770 t
ORIGIN      
        1 tatgactgtt ttgttattaa tcactttaac tgtttctagt aaaaaaaatt tcaatcaaaa
       61 aataatttct cagccgggac atattgttgt aaacaaagaa acagtagagg cgtataccac
      121 taaaggaatg gattattttt tatatgatta ttctcctatc gcttcattgg agaataagga
      181 gcatataata aaacacgaag caaagattgg cagtcaatgg gtaaaccttc cttataaaac
      241 aactggtagt tctatcatat atagagtaac taatgagaag agccaagtaa tcacacttcc
      301 tgtatatgct tatttaggaa cgaatgtaga aataaatcat caacctgtaa aggagcaggt
      361 gaatgctaat ggattggtac aaataagcgg agtcaaagga gttgctgata ttaaagtaag
      421 cacaagttat acagtaacga ctattatttc ttatcttgtt acattaactg catatttatg
      481 tttatttttt aaaatagttt tccataaaag gtaaattaac ttttcattta taaaataaat
      541 tgattaaaaa ggagaatctg tcgataagga ttctctcttt tttatacgaa aaatctgtag
      601 ggggatgaag taatcgctta tttcctcact ttatgataaa ttacagctaa agtcactagc
      661 ttgtcttctt cggaaaggag gagaatctat ggatgaggaa caaatattca ttcaaacact
      721 ttataatctg attttgaatc cgaatacacg agattgggaa cgaaaagtat tgattcagac
      781 aaagaatgac ataagagaaa atatatccgt taaagaacag ttatcaaaac tagaagcaac
      841 actacgtcca ttagctatcc gaatgaatct gacgcctgat gtaatggact tttatttatt
      901 gattacggaa ggatttgata aggagcaaaa atatgacttt tccaagcatg ccatgcaaga
      961 tgctgactat caagaacgtg cagtatttgc agggggatgt ttctggtgta tggtcgagcc
     1021 gtttgaatcg aaaaaaggga tcctatctgt tttgtcagga tacacgggcg gacatgttga
     1081 aaaaccaaat tatgatcaag taagtggtgg ttacacaggt catgtggaag ctgtggagat
     1141 catttatgac acaagagaaa taagttatag cgagttgctg acaatctatt ggcaaattac
     1201 tgatcccaca gatacatttg gacaatttca agatagaggg aagcaatatc gtccagtgat
     1261 tttttatcaa ggcgaacgac aaaaagaatt agcggaacaa tcaaaacaga aactagacag
     1321 ttcaggaact ttccaccaac caatcgtcac aaaaatcgaa ccagccggta cgttttggcc
     1381 agcagaaaac tatcatcagc aattttacaa aaaacaaccg aaacgttata aaaaaatcca
     1441 gcaagcacgt aatcaatttc tgatctatca acgagtaaaa aacaaatggc aaaaaaatat
     1501 acgaaaaaat cattttgatt aactcctttt ctttaaaatg tagtaaacta aaaatagaaa
     1561 aaagaaagcg ttttaaaaag atttgatgag gaggagaaaa attgtatatt gcagatagtg
     1621 cacgttatga aaaaatggaa tatcgtaaat ccggtcattc aggtttgaaa ttaccgattt
     1681 tatctctcgg attatggcaa aattttggtg attatgatcc tatccataat caaagagaga
     1741 ttttaagagg cgcattcgac atgggaatta cccatttcga ccttgcgaat aattatggag
     1801 gccctgctgg agctgcagag aaaaactttg ggcgtatttt tagagaagat tttcaagcct
     1861 accgtgatga actgattatt tctagcaaag caggttatca tatgtgggaa gggccatatg
     1921 gggaatgggg ctctcgtaaa tccatcattt caagttgtga ccaaagtctc caaagaatgg
     1981 ggctagatta tgttgacatc ttctatcatc atcgcccaga ccctgataca cctttggaag
     2041 aaacagcaga agctttgatg caacttgtaa gacaaggaaa agcattatat attggtatat
     2101 ccaactataa tggagaagat acaaaaaaaa tgactgaaat tttaaaaaga aaggaagcac
     2161 cgttcattat tcatcagatg cgctacaata tgttttctcg tgcactacta gaagacgatc
     2221 tatctccagt attagaagaa gaagggctag gggcaatcac gttcagtccg cttgctcaag
     2281 ggttgctgac aaatcgttat ctacatggta ttcctgaaga ttctagagca catcgtaagg
     2341 aaataccatt cctttcagaa gaacaagttg gatcaactct ggaaaaaatc aaagcactgc
     2401 aaacaatcgc cgtatcacga ggacaatcat tagcgcaaat ggcccttgca tggaatttgc
     2461 gacaaaaatc tgttacaagt gtactggtcg gagcaagtcg gttaagtcag ttacaagaaa
     2521 gtgttcgaat gatggataat ttagatttct caccagaaga agaattgaga atcgaccaaa
     2581 ttctagaata gaaacgatgt aatcaaactt tttatccata tattaaaaaa aacaaaagat
     2641 gacatcaaga atcgaatgaa gatcgtctgt tattgaacat catatttttt gtcgttcaaa
     2701 ataaaaatga tcgaaggcta ggagtgaatt tt
//
LOCUS       DAZULG010000193         2661 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000193 DAZULG010000000
VERSION     DAZULG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.200"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG9CY_002792"
     CDS             complement(<1..182)
                     /locus_tag="ACG9CY_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4939238.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG9CY_002793"
     CDS             517..951
                     /locus_tag="ACG9CY_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4939239.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG9CY_002794"
     CDS             974..1294
                     /locus_tag="ACG9CY_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939240.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG9CY_002795"
     CDS             1435..1626
                     /locus_tag="ACG9CY_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939241.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG9CY_002796"
     CDS             complement(1697..1954)
                     /locus_tag="ACG9CY_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS4939242.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG9CY_002797"
     CDS             2112..2375
                     /locus_tag="ACG9CY_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939243.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG9CY_002798"
     CDS             2399..2587
                     /locus_tag="ACG9CY_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939244.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
BASE COUNT         1017 a          382 c          548 g          714 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga a
//
LOCUS       DAZULG010000194         2509 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000194 DAZULG010000000
VERSION     DAZULG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.201"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>2509)
                     /locus_tag="ACG9CY_002799"
     rRNA            complement(<1..>2509)
                     /locus_tag="ACG9CY_002799"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          517 a          759 c          527 g          706 t
ORIGIN      
        1 gctcctctca aatttccaac gcccgcgacg gatagggacc gaactgtctc acgacgttct
       61 gaacccagct cgcgtgccgc tttaatgggc gaacagccca acccttggga ccgactacag
      121 ccccaggatg cgacgagccg acatcgaggt gccaaacctc cccgtcgatg tggactcttg
      181 ggggagataa gcctgttatc cccagggtag cttttatccg ttgagcgatg gcccttccat
      241 gcggaaccac cggatcacta agcccgactt tcgtccctgc tcgacttgtt ggtctcgcag
      301 tcaagctccc ttctgccttt acactcttcg aatgatttcc aaccattctg agggaacctt
      361 tgggcgcctc cgttaccttt taggaggcga ccgccccagt caaactgccc atctgacact
      421 gtctcccacc acgattagtg gtgcgggtta gagtggccat aacgcagggg tagtatccca
      481 ccagcgcctc catcgaaact agcgttccga tttctacggc tcctacctat cctgtacatg
      541 cggtacagac actcaatatc aaactacagt aaagctccat ggggtctttc cgtcctgtcg
      601 cgggtaacct gcatcttcac aggtactaaa atttcaccga gtctctcgtt gagacagtgc
      661 ccaaatcgtt acgcctttcg tgcgggtcgg aacttacccg acaaggaatt tcgctacctt
      721 aggaccgtta tagttacggc cgccgtttac tggggcttca attcgtacct tcgcttgcgc
      781 taagcactcc tcttaacctt ccagcaccgg gcaggcgtca gcccctatac ttcatcttac
      841 gattttgcag agacctgtgt ttttgataaa cagtcgcttg ggcctattca ctgcggctga
      901 tcgtatgatc agcacccctt ctcccgaagt tacggggtca ttttgccgag ttccttaacg
      961 agagttctct cgctcacctt aggattctct cctcgactac ctgtgtcggt ttgcggtacg
     1021 ggccgctgtt ttctcactag aagcttttct cggcagtgtg acatcaggaa cttcggtact
     1081 attatttccc tccccatcac agcttgtcct taagaaaaga agcatttgac tcctcttcag
     1141 acttactgct tggacagaca tttccgatcg tctgcattcc ttagcctcct gcgtccctcc
     1201 attgctcaaa caaaaacaac gggtacagga atatcaacct gttatccatc gcctacgcct
     1261 gtcggcctcg gcttaggtcc cgactaaccc tgggcggacg agccttcccc aggaaacctt
     1321 agtcattcgg tggacaggat tctcacctgt ctttcgctac tcataccggc attctcactt
     1381 ctaagcgctc cagcagtcct cacgatctgc cttcaacgcc cttagaacgc tctcctacca
     1441 atacacctaa aggtgtactc cacagcttcg gtaatatgtt tagccccggt acattttcgg
     1501 cgcagggtca ctcgactagt gagctattac gcactcttta aatggtggct gcttctaagc
     1561 caacatccta gttgtctgtg caaccccaca tccttttcca cttaacatat attttgggac
     1621 cttagctggt ggtctgggct gtttcccttt cgactatgga tcttatcact cacagtctga
     1681 ctcccggata tgaatgaatg gcattcggag tttatctgaa ttcggtaacc cgagatgggc
     1741 ccctagtcca aacagtgctc tacctccatc attctcaatt ccgaggctag ccctaaagct
     1801 atttcggaga gaaccagcta tctccaagtt cgtttggaat ttctccgcta cccacacctc
     1861 atccccgcac ttttcaacgt acgtgggttc ggtcctccag tgcgttttac cgcaccttca
     1921 acctggacat gggtagatca catggtttcg ggtctacgac tacatactca aacgccctat
     1981 tcagactcgc tttcgctgcg gctccgtctc ttcaacttaa cctcgcatgc aatcgtaact
     2041 cgccggttca ttctacaaaa ggcacgccat cacccattaa cgggctttga cttgttgtag
     2101 gcacacggtt tcaggttcta tttcactccc cttccggggt gcttttcacc tttccctcac
     2161 ggtactggtt cactatcggt cactagggag tatttagcct tgggagatgg tcctcccgga
     2221 ttccgacgga atttctcgtg ttccgccgta ctcaggatcc tcctaggtgt cttccacatt
     2281 tcgtctacgg ggtttttacc ctctttgact gacttttcca agtcattcga ctatctgaaa
     2341 gaactaccat attggagtcc tacaacccca acaagcaagc ttgctggttt gggcttttcc
     2401 cgtttcgctc gccgctactc agggaatcga attttctttc tcttcctgca ggtacttaga
     2461 tgtttcagtt ctctgcgtct acctcgaatg tgctatgtat tcacacaat
//
LOCUS       DAZULG010000195         2495 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000195 DAZULG010000000
VERSION     DAZULG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.202"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG9CY_002800"
     CDS             <1..963
                     /locus_tag="ACG9CY_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4939245.1"
                     /translation="PINTFAYTINNEFNLGPNEGSSQVANNQYILLHETANETATGRN
                     EAQYMKRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIYRPDVNQAYNTLGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
     gene            1267..1674
                     /locus_tag="ACG9CY_002801"
     CDS             1267..1674
                     /locus_tag="ACG9CY_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939246.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            1688..2089
                     /locus_tag="ACG9CY_002802"
     CDS             1688..2089
                     /locus_tag="ACG9CY_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939247.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            2091..2462
                     /locus_tag="ACG9CY_002803"
     CDS             2091..2462
                     /locus_tag="ACG9CY_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939248.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          913 a          373 c          463 g          746 t
ORIGIN      
        1 cccattaata cgtttgctta tacgatcaac aatgaattta atttgggccc aaacgaaggt
       61 agctctcaag tagcaaataa tcagtacatt ttactgcatg agacagctaa cgaaacagca
      121 acagggcgca atgaagcgca gtatatgaaa cgttcatgga ctagtgctta cactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc taacagtccc gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tagctagaga ttcagcaatg
      361 aaatatggta ttccacttac actagacaca ccgtataatc aaccagggat caaatcgcat
      421 ttatgggtaa cacaaaatat ttggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcctat gatttggctc atggatttac cgatgaaaat
      541 ccgacaactt cagatgataa accagtcatt gatccaactc gagcaggtgc tgcaaatcct
      601 accctgacag atggaacaaa ttacgcccac attgatcagt tcggagaaat cgaaaacgca
      661 aacttacatg tggctggttg gcacatcgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaaaagctg atggaatata tagaccagat
      781 gtaaatcaag cttataatac tttaggaaat gttggctatc atgtatcttt taacatgcgc
      841 aacttcccta ataagaaagt ctatgtaatg atgcgggcaa cgaatgatcc agaagggaac
      901 actaaaggcg gagcacaaga ttttcatgat aaacgctggt atttaaatat tccgcaacga
      961 taaaaatagc ccctcatttt tgaggggaag tacatatagt tgttgatata gttgctaata
     1021 ttgttagcta atttgttagt aaaactgtat acgaatttag atagtaaaaa aacagtttgt
     1081 tccttatata gaggcacttt tactactgat taaatgtcag atatgcttac aaatatactt
     1141 ataaatatgc ttacaaaatt agctaatatt tccgttgaca aagctttgtt actattgtta
     1201 aactaaattg actagcaaaa gtgaaacata aatttattaa ataaatgtaa aaattaggag
     1261 aaatcaatgt cttatagagt acaattaata atttcagaag atgtagaaag tcagcaattt
     1321 ggcactaatg ttattaataa agtaattaat ccagctcttt ctattaatgc gcctttaatt
     1381 cctactgctt tatcattttc tgtaacagct gtggtgtctg agatagagga tacagaaaag
     1441 ataaaaatag tagaaattga agttttaaac aagaatgaaa aacaaatttt ttctacgggt
     1501 gaagtatcgg taagcttgcc tcctcaagtt aacgatataa actttaatat aaacgccaga
     1561 aatgtcttgg ttgaagaagc aggagagcat tatgctgttg ttaaatttaa tggaactgag
     1621 attggaaggc aaatctttga tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac
     1681 gatacggatg cttgatatag taccatcaaa tacacatcgg agtggtagct taataaattt
     1741 tgctgcgatt tttgcttgcg catcggtaat tctagcatct ccagttgttg tggatgcaag
     1801 ttctactccg aaaactaaaa atgataatca acttgtaatt actacaaaaa atgagattaa
     1861 cacaatttca agtaatgatg gcaaaatttt tgatgtgata aatacggttt taaaaagaaa
     1921 agctgaaatc aatataaaat atgatgaaga tttaaacttg tatttctttg ttatcaaaac
     1981 aacatctgaa ttgttttcct cagattacga tgttttggat actctcgata atgtattatc
     2041 tgactataaa tatatgggga aatctgtagt agcaactttg ggggaataaa atgtttgact
     2101 ttaatgaata tttatctatt tccaaacaaa ttgaatcaga tgataaatat aattctaaag
     2161 aatcttatag aagaactgct gtttcaagag catactacag tgcttttaaa aaatctgatg
     2221 agtatttgaa agaaaattat gatattattt ataatggaag tagcggaaaa ggaagccacc
     2281 aaaccgtttg gaatttgttt tcaacagtta aggaattgaa taccttagga atacataata
     2341 gtgggtatag aatgttagag aaaagaaaat gtgctgacta tattcctaat gaaaccatta
     2401 cgaaaacaga tatggcgctg atgaatcgag aagcagaaaa aataataaac aaactcacct
     2461 aatgaatttc aagcgagttt gtttgttatt ttttt
//
LOCUS       DAZULG010000196         2418 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000196 DAZULG010000000
VERSION     DAZULG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.203"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..2193
                     /locus_tag="ACG9CY_002804"
     CDS             40..2193
                     /locus_tag="ACG9CY_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS4939249.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          830 a          430 c          540 g          618 t
ORIGIN      
        1 acaatgaagc atgaatgtaa ataggcggaa ggataggaaa tgatgaaaga ttttatgaaa
       61 aaaatattaa atacttcttg gttagaaaaa ataagaaaag gtgcacaaga acctaagaac
      121 aaaacgtttc gccggtccca tgttcctttt cgcttaaatt ttcttttttt cattatattt
      181 acgttatttg ttgcgttgat tgctagattg ggttatttgc agatcgtgaa cggcgaagag
      241 atggaggcgc gagtgaagtc tacttctacg attacggtcc aagaaagtac tcctcgcgga
      301 atgatctacg actccacagg aaaagcttta gttacgaata aagcgaatca ggccatcaca
      361 tatacgagag gaacacaagt tacggctaaa gatttgcttg aaatcgcaac gaagctgaac
      421 tcgctgatcg atgttccggt agatgaaaat ctgacagata gagataaaaa agatttttgg
      481 ctggctgatc ccaaacattt gaaagaagcc acaaaaagac tttcggcaaa agaaaagcaa
      541 ttagggaaca gcgaacaata tagtgcgatg gtcaataaag tcacagctga tgagattaat
      601 ttaaacgagg agcaattaaa aattgctacc atctttaaac ggatgaatag tgcatatgaa
      661 ttaaacactg tctatatcaa aaactctggt gttactgatc aagagttagc tgttgtggca
      721 gaacgcgctt ctgaacttcc aggagtttcg actggcacag actggacacg agaatataat
      781 gcagcaagtt cactgaaaag tattcttggc agtgtgacga ctgaaaagca aggacttcca
      841 gcagatgaag cagagaaata cctagcaaaa ggttattctc ggaatgaccg tgtaggacaa
      901 agttatttgg aaaaacaata tgaagatgtc ctgcaaggaa ccaaaaccca atatgaagtt
      961 tcccttgata atgaaggaaa tgtttcgaat caaaaagaaa tcttctctgg ggaaaaaggt
     1021 tctaatttaa tgttatctat gaatgcagaa ttccaatcaa aagtagaaga aattttgaaa
     1081 aggaattatc aaacattgat caacaacggc aaagcacagt attcgcctgg tgcttatgca
     1141 gtggctatga atcctcagac aggagaagtg ctagcaatga ctggtttcag tcatgaacaa
     1201 ggttccaaag aaatcaccga gaatgcttta ggcacgatta ctagcgcctt tgatccaggg
     1261 tctgtagtca aggctggtac tttgacagca ggctgggcaa gtaacgcgat cagcggaaat
     1321 caagttttga ttgatgagcc gattcgtctg caaggtgctt cagaaaaaag ctctgtcttt
     1381 aatcgaagcg gacaagttgc tcttgatgca gtcaaagcat cggaattgtc ttctaatacg
     1441 tatatgatca aagtggcgtt aaagatgtta ggactagact atacacctgg aatgggacta
     1501 cctagtttag atgaagaagc taaagcttat cagcaattga gagactcttt caaagagttt
     1561 ggtcttggta caacgactgg gatcgatttg ccgaatgaat ctcctgggat ctcacgttct
     1621 gttgattata tgaagaagtt caatgcggat aatggcaaag aatggtatgc tccaggtaac
     1681 tttaccgatt tggcatttgg acagtttgac acatatacac caatccaatt agctcaatat
     1741 gcttcaacag tcgctaatgg cgggaaacga gtacagccgc acctagtcaa agcgatctat
     1801 ggcaatgacg aaaatggaaa cttgggtgaa gtgaagaaag aaattggcac aacagtagaa
     1861 aatacggtca atatttctgc agaaaatatg gcgatcctcc gtgaaggatt ccaccaagtt
     1921 gtccatggta ccgatcctta cacaacagcc aaaccattag ccagtgcgaa aatggacctt
     1981 tccgcaaaaa cagggactgc agaaacggtt gcggaaggtc atccagatat tgcgacagtc
     2041 aatagtaata tcgtggcata cggaccaaca gacaatccgg aaattgccat cagtgttgtc
     2101 ttgccgaact tattagacga acaagaccac ataaacttga cgattgctaa agaaataatg
     2161 gacgcgtatt atgatatgtt catggcaaaa taaaaaagag gtcgtgaaaa agccgcttag
     2221 tttcgagtat gaagaaaaat tttgtgaaaa tagcttctca catttttgct taaatttgac
     2281 ttcataccga gaaactggct tttgaacacc gtttactcgg caaaaaaggt tgtgacaaaa
     2341 attgtggctc tttgtcaata aggactgatg agttgtgcaa aatcaaatct gagtcagaat
     2401 gaaccacatt ctggctca
//
LOCUS       DAZULG010000197         2410 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000197 DAZULG010000000
VERSION     DAZULG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.204"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..223
                     /locus_tag="ACG9CY_002805"
     CDS             <1..223
                     /locus_tag="ACG9CY_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4939250.1"
                     /translation="KYLKTGSRIEFPDGSTPIAYLAALLNTLLRYDSLINDSEKNTDE
                     KKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            284..1429
                     /locus_tag="ACG9CY_002806"
     CDS             284..1429
                     /locus_tag="ACG9CY_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4939251.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(1510..1815)
                     /locus_tag="ACG9CY_002807"
     CDS             complement(1510..1815)
                     /locus_tag="ACG9CY_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS4939252.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(1840..2271)
                     /gene="ruvX"
                     /locus_tag="ACG9CY_002808"
     CDS             complement(1840..2271)
                     /gene="ruvX"
                     /locus_tag="ACG9CY_002808"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS4939253.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(2268..>2410)
                     /locus_tag="ACG9CY_002809"
     CDS             complement(2268..>2410)
                     /locus_tag="ACG9CY_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS4939254.1"
                     /translation="VGYLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGV
                     NL"
BASE COUNT          792 a          483 c          417 g          718 t
ORIGIN      
        1 aaaatatctt aaaacaggat ctcgaattga atttcctgac gggagtaccc caattgctta
       61 tctcgcagct cttctaaata cgcttttacg atatgactca ttaattaatg attcagaaaa
      121 aaatacagat gagaaaaaag agtatcaaga attttatgaa aatgatcttc taaaagagtt
      181 cacagctctt gttactgagc taaataaaca ctattcaaaa taataataaa ctgcctcctc
      241 caaccgcccc cacggcttgc tacactatgg agggaaaact attatggcaa cattcaagca
      301 atatgaaact aaaaaaggta aattttggct ctatgaagcc tatcttggaa tgaacaaaat
      361 gactggcaaa cctgataagg ttcgtagacg aggttacaaa acaaaaaaag aagcccagct
      421 tgccttgtca cgcttgcaag tggactacga taaaaacggt attaaaaaag cgaacaacga
      481 aacgtttaaa gaagtttatg acttgtggct agaaacatat aaaaccactg tccgagaagc
      541 caccttcatg aaaactgaaa tcaagtttag aaagtggatt cttccaaaat atggaaatct
      601 tcgtgtgaat gaagtcacgg taaaaaaagc ccaacaaatt gtaaatagct gggctgaaac
      661 tactgaccaa tacaaggtac ttcattcaac ttctaagcgt atttttagct acgctatcaa
      721 tcttgggatt attgagacta atccactaga caacgtatta atgcctaaaa gaaccaccac
      781 taagcataaa gaaaaggtca aagtttacag taaagaacaa ctgatcacct tgtttgatta
      841 cctaaacagc aaacctgaaa cttatcgaaa cgactttgat aaaacgttac tacgctttct
      901 cttttatagc ggggttagga tcagtgaagc ccttgcattg aattggtcag acattgattt
      961 tcaagaaaat acggtcacca tcaataaaac actaagccaa tccaaaaatg gctataaaat
     1021 cagtgatcct aaaactgatt gtagtgctgg ggttcttcca ctagatgaaa gaacaatttc
     1081 tatattaaaa aaatggcaga ttaatcagcg gaaatatatg ctgacacttg gtgtaactga
     1141 tccgactatg attttttgtg gtatctataa agaactaatc acacaccacg ctatttatgc
     1201 aagattgatt actatttgtg aaaaagctat ggttccattt ctcggtgtgc atgtagcaag
     1261 acacactcat gcttcaatgc tcttggacgc tggagctact atggtagaag ttcaaactag
     1321 attacggcat acaaaaatta gtcagacgat cgattgttac ggtcacttgg caaaagaaac
     1381 gaaagaaaag actgttgaaa aattagtaca acacttaaat attgactaga tggcatacaa
     1441 tatggcatac agtaaaaaat aaaaaaagga gaaaccctat catatcaagg tttctcctag
     1501 ctttatagtc tattcttctt cttccgccat gaatgtattg aatacttctt caatcatgtc
     1561 ccattctgcg tctgtttcga tttgttctaa ctcgccttct gttccatctt cgttttcgat
     1621 gtatgcataa gcttgtaatt ctacatcttc atcttctgaa acaccagcag gatataaaag
     1681 aacatagtta cgtccaaatt cttcttgtcc gtcaactgtt agtaggattt catataatgt
     1741 ttcatttcct tggtcatcca ccaatgtgat gtgttcgtgt ccttcatggt cgtgggaatg
     1801 gttatgttct gtcataagat tacctctttt cttttattat taaaataatt gcttacctga
     1861 gccgtctaaa taattttgta agatcatcac agctgctaat ttatcgatga ctttttttcg
     1921 tttggctctt gaagtatttg cctgctctac tagcatacgt tctgcctgaa cagtggttaa
     1981 tcgttcatct tgataatcaa ctggcaagga gaatagttct ttgatctttt ctccgtatga
     2041 catggaagct tctgcccttg gtccgatgga attgttcata tttttaggta atccaactac
     2101 aaaccggtct acttcatatt gtgctactaa ttccttcaac cgatcaaagc caaactcttc
     2161 ttctgcttca ttgatccgaa tgatctctac tccttgagct gtccagccaa aggggtcact
     2221 gatagcaaca cctactgtct tagaaccgac atccaagccc attactctca taaattcact
     2281 ccatgatttg agaggtagtc cttgacaatt acttccatga tctcatcgcg ttcgtgacgg
     2341 cgaatcaaat ttcgagcatc acgataacga ggaatgtagg acgggtctcc agaaagcaag
     2401 tagccgacaa
//
LOCUS       DAZULG010000198         2400 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000198 DAZULG010000000
VERSION     DAZULG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.205"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG9CY_002810"
     CDS             207..2069
                     /locus_tag="ACG9CY_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4939255.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACG9CY_002811"
     CDS             2069..>2400
                     /locus_tag="ACG9CY_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4939256.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZULG010000199         2396 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000199 DAZULG010000000
VERSION     DAZULG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.206"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..1001
                     /locus_tag="ACG9CY_002812"
     CDS             18..1001
                     /locus_tag="ACG9CY_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4939257.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            1031..1153
                     /locus_tag="ACG9CY_002813"
     CDS             1031..1153
                     /locus_tag="ACG9CY_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4939258.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            1171..>2396
                     /locus_tag="ACG9CY_002814"
     CDS             1171..>2396
                     /locus_tag="ACG9CY_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFS4939259.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFV"
BASE COUNT          920 a          357 c          474 g          645 t
ORIGIN      
        1 ggtaaaaagg tgacaaaatg ggcataccag ttacaatcgt ttctgggttt ttaggggctg
       61 gaaaaacaac gctaatcaat caagccttgc aagtctcacc ctaccccagg gaagaaatta
      121 ttataattga aaatgagttt ggtgaagttg ggattgatca caaattatta gttcatagcg
      181 aggaacgggt gttacaacta aataatggat gtatgtgttg cagtcttcgc agtgatttat
      241 tagcaatatt gatttcttta ctggaaacaa tagaagaaca tcaacaacca atttcacaaa
      301 tcatcattga aactacaggt atagcagatc cccaaccaat cattcaaaca cttttaacag
      361 cgcctcaact aagaggaaga gtatatatcg atagtttatt gactgttatt gatagtgaca
      421 gttttgaact tattgaaaga gaaccacaag cccttaagca acttgtcatg gcagatcgca
      481 tttttgtgtc tagaaaacaa aacttgaatt tatctgcact aaaaaaatta cagagagaaa
      541 tcaaaagtat taacccttta tccgatattc gaatgttcac acatagtgaa acgattcaaa
      601 aaaaggattt ttataatttg aaaaaattta atcatccctt agtttttgaa agtgattcat
      661 cagaaaatca ctataatggc aaatactatc atcaacacca tcaagtacat gagtttaaat
      721 caattctctt aacttcagat caagacataa aaaaagattt tttactccaa tggattgact
      781 ggctgatttt aaccaaccaa gggagcctgt atcgttttaa aggattgatt gctttacaag
      841 agcaggattt tatagttgcc atgcaagagg taaacgaaca ggttaatttt caatacacca
      901 ctcgacaaaa agaccaaacc actattattc taatcggaaa aatgttggat gaagaaagga
      961 tcaaacagag ttttgataaa ttactggcgg aaagcaaata atgataagta gtgattgggg
     1021 gtaatttcta atggagaaaa cggatatatc tagtgcatat cgacgactta aaagcccgaa
     1081 tattaaaact agaaaaaggg cattaaaaat tatcaaagaa cataaacgaa ataaaatgaa
     1141 gaaattagca taattaagga aggaaaaaga atgaagaaaa tcagtgtagg acttattggg
     1201 atagcagctc taggattatt gggagcttgc tccagcacta atgatgcaaa agtatcgaat
     1261 gataaagatg gaaaattaga aattgtaaca acattctatc caatgtatga tttcaccaaa
     1321 aacatcgtcg gcgatgaagc gaacgtggat ttaatggttc cagcgggatc agaacctcat
     1381 gactatgagc cctcagctaa ggatatggca aaagctcatg atgctgatgt ttttgtatat
     1441 cataacgaaa acatggaatc atgggtacca aaagctaaag aaagttggaa gaaagctggg
     1501 cctaatgtag tagaaggaac aaaagacatg attttattgc caggtagtga agaagaagat
     1561 catgatcacg gagaagaaga tcaccatcat gaactagatc ctcatacgtg ggtttcaccc
     1621 aaaatggcaa ttaaggaagt ttcaaatatc aaggatcagt tagttaaatt gtatccgaaa
     1681 aaagcaaaag tatttgagac aaatgctgaa aagtatttga ctaaactgaa acggttggat
     1741 gctgactata ccactagtct taaagaagcg aaacaaaaaa gttttgtgac gcaacacgca
     1801 gcctttggat atttagcttt ggattatgga ttgaaacaag ttcctattgc tggactaagt
     1861 cctgaagaag agccatcttc tggtcgatta gctgaattaa aagagtatgt taaaaaaaat
     1921 aagatcaatt atatttactt tgaaaagaat gctaatgata aaattgcaaa aactttggcc
     1981 aacgaagctg ggataaaatt ggaagtactg aatcctcttg aaagtcttac aaaagaacaa
     2041 atggataatg gtgaagatta tgtttcagta atggaagaca atctaaaagc gttagaaaaa
     2101 acaactatgg ttgctggtga agaggtagtt ccagaaaaag aagcgaagga tgaaaagaca
     2161 gttgctaatg ggtactttaa agatgccgat gtgaaggatc gcgaattgag tgattataca
     2221 ggcgaatggc aatctgttta tccgttgtta aaagatggaa ttttggatga agttttcgat
     2281 tacaaagcaa aaatcaataa agacatgaca gctgccgagt acaaagatta ttatactact
     2341 ggctataaga cagatattga tactatcaac attaaagaca acaccattga ttttgt
//
LOCUS       DAZULG010000200         2385 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000200 DAZULG010000000
VERSION     DAZULG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.207"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..473
                     /gene="dctP"
                     /locus_tag="ACG9CY_002815"
     CDS             <1..473
                     /gene="dctP"
                     /locus_tag="ACG9CY_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HFS4939260.1"
                     /translation="QSETAIKMVELLGGSPVPMGSDEVYTSLQSNLINGSENNEFVLY
                     TAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAKESTVFEIEAFAK
                     AIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLYQKIQEQGA"
     gene            479..958
                     /locus_tag="ACG9CY_002816"
     CDS             479..958
                     /locus_tag="ACG9CY_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS4939261.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            974..2269
                     /locus_tag="ACG9CY_002817"
     CDS             974..2269
                     /locus_tag="ACG9CY_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS4939262.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            2280..>2385
                     /locus_tag="ACG9CY_002818"
     CDS             2280..>2385
                     /locus_tag="ACG9CY_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HFS4939263.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLA"
BASE COUNT          661 a          418 c          553 g          753 t
ORIGIN      
        1 tgcaaagcga aaccgctatc aaaatggtag agcttttagg cggttcgccc gtacctatgg
       61 gaagtgatga agtgtacacg tctcttcaat ccaatttgat caacggatca gaaaacaatg
      121 aatttgtctt gtatacagct ggacatggtg gtgtggctaa atactattcc tatgatgagc
      181 atacacgtgt gccagatatc atcatcatga atgatgccat caaagaacag ctaactgatg
      241 agcaggaaaa agccattgaa gaagcagcga aagaatcaac ggtttttgaa attgaagctt
      301 ttgcaaaagc aattgaagaa gaaaaagctt tagcacaaga agaacatgga gtccaattca
      361 atgatgtaga taatacaccg tttcgagagg cagttgcccc acttcatgat atattcaaaa
      421 acgatcctac ctacaaagac ctttatcaaa agatccaaga gcaaggagcg tagctgtgat
      481 gaaaatgatt ttaaacaagg tattagagat cctgggagct gttttgcttg ttgggatggt
      541 cgctgttgtt ttatggcaag tattttccag aacggtttta ggaaatccaa atacggtcac
      601 ggaagagctt gttcgattcg gtcttgtctg gtttgccatg ttgtcttccg cgtatgttgt
      661 gggacaaaaa gcacatttag ccgtaacggt tttaagtgaa aaattaactg gcaaaaaaag
      721 taaaatttta gaagttgttg ttcaaagttt atttctggta tttgctgcga tgatcatggt
      781 gtatggcgga tggaatgctg tcacattgac aatggggcag atctcgcctt ctttaagtat
      841 tcctatggga tatgtttatc tgtcagtacc tgtatcgggg gtactcatca ttatctatag
      901 cctgatcaat ctaatcgata cattaagcaa caagccagca attcaagaaa aaatataaaa
      961 atggaggaat aaaatggcaa tagaagccgg attagtgtta ttttttgtat ttgttgtgtt
     1021 gcttgtatta gggatgccga ttgctataag tattgcattc tcatcgatga tgactttatt
     1081 gttagttata ccgtttgatg tttctgcttt tagttcagct caaaaaatgg tgggaagtat
     1141 caatagtttt tcgctgatcg ctattccgtt tttcgttctc tcgggcatta tcatgaataa
     1201 tggagggata gctaagaaac tagtggattt tgcgatgttg ttcgtcgggc gcgtgccggg
     1261 ggctttggct catacgaatg tgttagggaa tgctttattt ggttccatct ctagctcggc
     1321 cattgccgct tctaccgcga ttggcggagt gttgatccct cagcaagtgg aagaaggata
     1381 tgaccggaag tttgcgacag cggtcaatat cgcgtcagct ccgacaggta tggtcattcc
     1441 tccaagtact gcttttatca tgtattcact tgttgctggt ggtgcttcga tctctgcttt
     1501 gtttatggga ggttatctcg taggcgcttt gtgggcgcta ggaatcatgc tagtagcatt
     1561 catcgttgct aaaaagaata aataccctgt tgtcgaaaaa ggacagatgg ctcatgctgg
     1621 aaaaataata aaagaggcga ttcctagcct tctattgatt gtcatcatta tcggtggtat
     1681 cttgacgggg atatttacag cagtagaagc ttcagcgata gctgtcattt actccctgtt
     1741 gattgctatg ttttactata aaacggtaaa actaaaagat atacctaata tgttgtttca
     1801 atcagtagca atgtcaggga cgatcatgtt cttattggct acttcttcca tgatgtcttt
     1861 cgccatggcg tttaccggta ttcctcaggc aattagttcg ttgattatgg gcgtaacgga
     1921 taataaattt attattctgt tattagtcaa tcttgtgtta ttactggtgg gtatgtttat
     1981 ggatgtggcg cctgctatct tgatttttac accaatattt ttaccaatcg tcacaagtgt
     2041 aggggttgat cctgtacatt acgggttgtt ttcaatcatg aatctgtgtg taggctccat
     2101 cacgccgcca gtaggtacag gactatatgt cggtgcaagt gtaggaggcg taaaagcaga
     2161 ggcaatgctg aaaccgcttc ttccttttta tggggttatc ttagtcgtct tgtttttgat
     2221 tacttacttc ccgcaaatcg tcatgtggct acccaatatg ctgagttagg agaatagtta
     2281 tgttgaacat tacaattaga ggacatgatc tttccgaagt ccaaacgata gaagatcttg
     2341 cagaaaaaac aaaagaacaa gggattcata cactgcagct cgcct
//
LOCUS       DAZULG010000201         2379 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000201 DAZULG010000000
VERSION     DAZULG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.208"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            213..1826
                     /locus_tag="ACG9CY_002819"
     CDS             213..1826
                     /locus_tag="ACG9CY_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4939264.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          749 a          422 c          453 g          755 t
ORIGIN      
        1 catcattttt cttcagcttt taaaaaatat acagggttat ccccttcaaa gtattatcta
       61 tccttaaaat agaaaaaaat ttttaggttt tatccacaaa ttatttctct atattttctg
      121 tctattcaaa ataaacaata attttccggc acaattattt gctgtttttt accataacat
      181 agtacgatca acgtagaaaa ggagatgttt ttatgaaagt tgttgtcatt ggcggtgtag
      241 ctggcggtcc ttcatttgcc actcgtttcc gtcgattgaa tgaagcgcac gaaatcatta
      301 tctatgaacg cggagaaaat atttcttatg caagttgtgc tttgccttat tatttaggag
      361 gtgtgatcac tgaccgtgac tcgctgatcg aacgtacacc agaaatattg aaaacaaaaa
      421 acaacatcga tgtctttact aaacacgaag taacagcaat cgatccttct actaagcgat
      481 taacagttaa agacctatcc acaaatgaag aaataaaaac agattacgat aagttgatca
      541 tctcttctgg tgctagacca gattatccgg atattcccgg agtttttgaa gcagaaaacg
      601 gctttgtcct ccgtagtgtg acggatgcgg atcgaatcaa atcgttcctt gaagaaaaaa
      661 atccacaaca tgtcgtcatt cttggtgcag gtgttatggg tctggaatta gctgagaatt
      721 tcaagcatcg cggcttagac gtgactttaa tcgatcaatt gccacaagtc gctttccctt
      781 atgatccaga aattgctaat ttagtttatg acaaattgct gaaagaagga ttagccgttc
      841 atttagaaac aagagttact gagatccgtg ataaaggtcg agaaatagta ttatcagatg
      901 gctccgtcct ttctgctgat atgctaattt ttgctgttgg tgtttctccg aagaatgaag
      961 tggtgaaagc aaccggcata caattatcag atacaggtca gatcattgtt gatgaccagt
     1021 tacaaaccaa tctgccggac atctatgcga ttggcgatat tatcgaaacg actagtgtag
     1081 tgactggtca gccgatccaa agtatgcttt ccagtgcggc caatcgtcaa ggacatatgc
     1141 tggcagatat tttaaatggt acgcctatgc gctatcgcgg atatattggt gcaggtgtcg
     1201 caaaaatctt tgatcataca gcaagttatg cgggaatgac agaacatgaa ttaaaagcat
     1261 caggcataac aaattataaa actgttttta tcactccctt tgaccatgcc tatttctatc
     1321 cagaagctac aagattaaat ctaaagctga tttttgatgc agatagcggt cgtattttag
     1381 gtggacaagc atttggagaa aaaggtgtcg ataaacggat gggagaactt tctgtagcaa
     1441 tcaccggaaa cttgacagtc tttgatttgc ctgatttgga gttgccttac tctccaccgt
     1501 attctactac ccgtgacccg ttgaatatag ctggttatgt cgcaatcaat caaatgacga
     1561 atatcgtaga aacgatcaaa gcaagtgata tacccgaaaa cgatttgaaa gaagcgttct
     1621 ttttagatat acgtgaacct aataaagcac cttctggcag tatctcggca acaaaaaata
     1681 ttccaatgaa tgagttacga gatcgaatca atgaaatccc aaaagataaa aaagtttaca
     1741 ttactttcag aagaggattg aatacttata cttctgcccg aatcttggca ggtttgggta
     1801 tcaaagcgat tttgattgaa gaataaaaaa acgaccgcgg tgggtagccg cggtctagga
     1861 gttattttta cttgaggagt aaaaatgaaa aagtatatag ggttgttggt tggtatgtat
     1921 atagaataga agataaatgt gaagaaattg tgcattattt cttcacattt attaggttat
     1981 agtttaagaa agtttaaaca ttgcgcaatt taattatttt agctgtttct tttcataaaa
     2041 aaacatgttg tgactctgag acaaatcata gtccatagtg atgtctctag agtaactacg
     2101 cctaatatta cgaggcatca tattaatcta tcagcactag tacctaaact agcatccctt
     2161 tcgaatattt tttcttatat aataaagttt gctttcactc ctctaatctc accaaatcga
     2221 ctggcttgcg ttgtgtgata ctaggaactg attggtttag aacgactttt ctattgtaat
     2281 catatttttc gccatcctcg ttgtcttcta atccttcaat gaatgatccc taatgtgtgt
     2341 attaaataat ttaatttttt tgttcctatt tatttaaca
//
LOCUS       DAZULG010000202         2293 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000202 DAZULG010000000
VERSION     DAZULG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.209"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACG9CY_002820"
     CDS             complement(51..1598)
                     /locus_tag="ACG9CY_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS4939265.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG9CY_002821"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG9CY_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4939266.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="ACG9CY_002822"
     CDS             complement(2043..2237)
                     /locus_tag="ACG9CY_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939267.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DAZULG010000203         2270 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000203 DAZULG010000000
VERSION     DAZULG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.210"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1056
                     /locus_tag="ACG9CY_002823"
                     /pseudo
     CDS             1..1056
                     /locus_tag="ACG9CY_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1182..2261)
                     /locus_tag="ACG9CY_002824"
     CDS             complement(1182..2261)
                     /locus_tag="ACG9CY_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4939268.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
BASE COUNT          921 a          428 c          396 g          525 t
ORIGIN      
        1 agaaacagcc aaatggacag aaaccaagtg ggccaacgtc aatcgaatta cctttcagga
       61 tgaattttca tccttcaatt caggagtcgc aaaattaaga aaaggacaaa cagcgaccat
      121 caaagcaaaa attgactatt cgggagactt agcacctata ctacaatcag ctgttacatt
      181 tgagaatgtt gatcccactt tgtcgattgg ctatgacgag aacactttga ctatccaaca
      241 caagcaagcg atatttacct tcaccgttac attaaatcag gacttgttaa aaccagcgct
      301 ttttactgtc aaggtatcag atggactacc gaacgatcat catttaacgc cgtatagtca
      361 gcggcagaca attgagcaag attcagccat cgactcagga ggagatcttg ttgaagaacc
      421 aacagataaa ccagaaaatg aaaataagcc agaagtaccg cctacagaaa atccagacgg
      481 agaacagaaa ccggaaatag aacctggaga agaacccgat acagaaactc aaccagagcc
      541 agacaataaa tcaaaacctg aaataacccc tggagaaaaa ccagatgtaa ctccagaacc
      601 agatacggat tctggaaacc aaacagtacc ggaaacaaat ccagacacag ataatgaaac
      661 tgaaaatcca gaaaaaccag aggtagatcc agaagaaaaa ccagatgtga ctccagaacc
      721 agatacggac gcaagagacc aaggaatacc agaaaaaata aacaaaaaaa caattcaaga
      781 ggatggaaaa aaagagagta aaaaatcaaa tctagcaatt ctaaaaataa atgaagagca
      841 gctgaataaa aaatcacgaa tatttgatag cgcccaatca gctgaaacct taaaaagtag
      901 caaggataca acctttgctg tcctgaaacg aaaaataaac agctaccaaa atccggagag
      961 tcacaaaaca aagttatact gtggagcgga atcattctat taagtatagc aacaatgctt
     1021 tctgcaaaaa gatttaaaca aaacagatct ctctaacatt tgaataatgt ctgtgaacgc
     1081 atttttcaaa taaaaagaaa tatccagaaa atgaccccaa aataccagat tgaaatctga
     1141 tatttggggt cgttcatact ttatctttct tcatatttat tttaagggat atctgtttct
     1201 tttggaagat ggttcgggat ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc
     1261 ggaatgccga caatcaagcc ccaaacgcca aagaaccgtt cgctaacaag taagaccaca
     1321 aatgtataaa aaatcggcag ttctgttttg ctggacatca acttcggatt cagcacataa
     1381 gactcaatta gatggacaat caaaatcgta atcaggatat aaacaacatc tcggatgcct
     1441 ccttgggaat aagctatgaa agatagagga atacaagaaa tgataacacc tgctactggg
     1501 atcagactta aaatgaaaat catgatcgct aaactcagca gctgatgaaa gccaaataca
     1561 gccaaggcga ttgtagtgag caacgtattt aaaagagcga caacaaattg agcttctaat
     1621 accaaaccaa atgtattgac aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa
     1681 gaaaattcac tttttaaaaa caatcttgag aatacaattg tttttttctt ttcaatcata
     1741 aaaaagaaac ttaagatgaa tgacatagca aacgccactg ccatttttcc tatatcctgt
     1801 aaatagccaa gcgcaatcga agcgccattt tgaagctgtt caaaaaaatc gtttcgctca
     1861 atataagtat ggatatattg taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa
     1921 tcaataacgg actggatcat atcaaatgtt tgatgaataa gcatcgggat atactttgtg
     1981 aatgccagat agataaggta aacgatcagt gtataaatca tgatagctaa aacacctgta
     2041 gggattcgga caaatcgctg aatgatttga acaagatgaa tcatcaaata agtaaagata
     2101 aatgtgagca aaatggttgt aatcatactt ctaactaaga aaagaacaaa aacgacaaat
     2161 aataaaacag tgagacggcg tattttttca ttcgccaaaa aacgctgata aacggtcaaa
     2221 tcgacaacct ccaaataaca aaaaataaac acgttattca ttatatgatg
//
LOCUS       DAZULG010000204         2263 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000204 DAZULG010000000
VERSION     DAZULG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.211"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2263
                     /gene="esp"
                     /locus_tag="ACG9CY_002825"
     CDS             <1..>2263
                     /gene="esp"
                     /locus_tag="ACG9CY_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS4939269.1"
                     /translation="SKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTTEL
                     VQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTGDT
                     LKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFVRI
                     NDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDNLK
                     TDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVSAK
                     YEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVPYV
                     AGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFANA
                     SPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQNTY
                     GTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGTGH
                     KAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSPGF
                     DKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSNVT
                     DSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDKPD
                     VPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQDGS
                     VGTTDLAVTVSKDI"
BASE COUNT          818 a          349 c          463 g          633 t
ORIGIN      
        1 agcaagaata ataagagagt atttctagaa aaaacaaaga aacgagtatt gaagtacagt
       61 atcaaaaaat taagtgttgg ggttgcatct gttttggttg gtgttggtct tgtcttggga
      121 acaactgaac tagttcaagc acaagatgaa atttctccaa gcaccccact tgaaacagcg
      181 ataagctccg tacaagtagg tgacaaagta gcttcgggga atacttttca ggagaatcct
      241 ggttatacga aaaattataa cttttcggat ttacaattta gtcctcaaga actaactgga
      301 gacacactaa aaggcaatac aattggattt gaagtttacg gaaaacataa tattgcagca
      361 tcaactaaaa actgggaaat ccgtcttcaa ttagatgaac gattagctaa atatgtagaa
      421 aaaattcagg tcgatccaaa gaaaggaatt ggatcttcta gacggacctt tgtaagaatt
      481 aatgattcgc tgggtagacc tacaaatatt tggaaggtca attatattcg tgcaagtgat
      541 ggattatttg ctggggctga aacaactgat acacaaactg ctcctaatgg ggtaattacg
      601 tttgaaaaga gcttagatga aatttttaaa gagataggca tagacaatct caagaccgat
      661 cgtttaatgt atcgaatcta cttggtaagt catcaagacg atgataaaat tgtaccaggt
      721 attgatagta cgggctactt tttaacagat tcagatgatt tctataatag cttagatgta
      781 tctgaaaata atcctgacca gtttaaacat ggatcagtaa gtgcaaaata tgaggaacct
      841 aatactcaaa caaaagatgg ttcaggatcc acaggtgcta atggagctat aatcttagat
      901 cataagttaa cgaaaaatta taatttctca tattcagctt ccgcaaaggg cacgccttgg
      961 tatgctaatt acaaaattga tgaacgatta gttccatatg tagcgggtat acagatgcac
     1021 atggttcagg ctgacaaagt aacatatgat gtttcttttg aatcagggaa aaaagtggct
     1081 gatttagcaa ttgaacggcg caaagatcat gaaaattatg gtgtaggttc aattactgat
     1141 aatgatctta ccaaacttat tgattttgct aatgcaagtc cacgtccagt cgttattcga
     1201 tatgttttac aattgactaa gccattagat gaaatcttag aagatatgaa agcaacagca
     1261 caagttgaag aaaataaacc atttggtgaa gatttcatct ttgattcttg gttgtcggat
     1321 acgaataaaa aattaatcca gaacacttat ggaacaggtt attattattt gcaagatatt
     1381 gatggtgatg gaaaccctga cgataaagaa gagagcggag acacgaatcc atatatcggg
     1441 aaacctgaat tagaagaagt atatgatgtt gacacaacag ttaaggggaa agtattcatc
     1501 cacgagttag cgggaacagg tcacaaagcc caacttgttg ataaagaagg tactgtatta
     1561 gcagaaaaaa ctatcgctcc aaatgaaaaa gatggggctc caatttcaga tactgtagaa
     1621 tttgaattta cgggtgtaga ttcaagtaaa ttaatcgcga aagatgaatt aaaaatccaa
     1681 atcgtttctc caggttttga taaaccagaa gaaggttcaa ccgttattaa ggaatcacca
     1741 aaagcggttg ataaacaaac cgtggtagtt ggatttaaac cagatgctaa agaatcaatt
     1801 cggaataata aaaacttacc tgaagatgca gagtattcat ggaaaacaga gcctgatact
     1861 tctaacgtta ctgatagtac gaaaggtatt gtaactgtta agatcggaaa tcgaactttc
     1921 gacgtggatg tagagtttgc tgtaaaagct tctcaagcta tggaaaatga tgcaacatac
     1981 gtacctataa caacaactcc agaaacgaca gttcaaagtg gaaaacctac atttgataaa
     2041 ccagatgttc ctttagctaa agatgccttt tcaattttag atgtctataa taaggacttc
     2101 ggtaatgcaa gtgttgatgc aaatactggt attgttacat tcactccagc taaaggtgta
     2161 ggagaatcgg agccgattac tggaacaatt cctattaaaa ttgtttacca agatggttct
     2221 gtaggcacga ccgatttagc agtaactgta agtaaagata ttt
//
LOCUS       DAZULG010000205         2148 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000205 DAZULG010000000
VERSION     DAZULG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.212"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..1239
                     /locus_tag="ACG9CY_002826"
     CDS             70..1239
                     /locus_tag="ACG9CY_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4939270.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
     gene            complement(1564..>2148)
                     /locus_tag="ACG9CY_002827"
     CDS             complement(1564..>2148)
                     /locus_tag="ACG9CY_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4939271.1"
                     /translation="PLASLKIILENMKYNIGKYKERDIYINECIEIVDSLTKNISQIL
                     SVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTALKII
                     LSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKENSNG
                     LGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          759 a          341 c          291 g          757 t
ORIGIN      
        1 gggtagaaaa aaaagtccat tctgtaaaat taaagttacc acacagaaat tttataggag
       61 gactttctca tgactaaatt tactatagaa attatgcaaa cactactcaa taaaggggat
      121 ttagatgaat tatttcagga tcatttagag cgagcgatta attcacttct acaagcagag
      181 ctaactgctt ttttagatta cgaaaaatat gatcgaaatg gattcaattc aggaaattcc
      241 cgtaacggga attattcacg tacatttaaa acagaatatg gcgagttgaa tttaactatt
      301 cctagagatc ggaatggcga attttctcaa caaacactac cagcatataa gcgaaccaat
      361 gattcattag aaacgaccat tatccaatta tttcaaaaag gcattactat ggctgaaatc
      421 tccaaattaa ttgaaaaaat gtatggtcac cactatacgc cacaaacaat ctccaacatg
      481 agtaaattgg tggctgaaga tgttttagct tttaaagaaa gaacgttaga agccaattat
      541 tccgttatat ttatggatgc gacgcatatc cctgtgaagc gacagactgt ttcgaaggaa
      601 gcagtgtata ttacgattgg tattcgtttg gatggaacca aagaagttct tgggtttact
      661 attgccccaa ctgagtctgc ttatatttgg aaagaagttc ttcaagatct tagaaaacgt
      721 gggttagaag aagttttatt agtagtgaca gacggattaa gctgtattga agaaagtatc
      781 catagtgtgt atccgaatgc ccaatttcaa caatgttgtg tgcatgtatc tagaaatatc
      841 gctcataaag ttcgtgttcg aggtcgaaaa gaaatttgtg aggatttcaa attggtttac
      901 caagcgaatt caaaagaaga ggcattggat cacatcgact ttatgactag gaaatggaaa
      961 aagcagtatc caagagtcgt caatttactc ttgaatcctg ccctattaac cttttataat
     1021 ttccctcacg ccatcagacg aacaatttat tcgacgaacc tgattgaagg ctttaataag
     1081 cagctaaaac gatatactcg aagaaaagaa caattcccta atgaagaatc tctagagaga
     1141 ttcttcattt ctcaatttaa tcaatataac caaaaatttt taggtagaat tcacaaggga
     1201 tttaaagaaa ttcaggatac attagagtcg atgatttaac tgtaattaac agaatggatt
     1261 ttccatttac acataattct tgacgctacc ataatctaaa taaccatcat attcatctac
     1321 tacttatata attgagtttt attaaacttt ttaagtttct attctatcaa atatttaaac
     1381 aatctctgca tccgaaaaga ctacctcgta tagttttact aacaatattt ttattagcgt
     1441 gttcaaatcc tgatttcttg tatcaattat tttctgttca ctttcaataa gaaaaaagtc
     1501 tttacttcca tggataataa taagtctcta tttttcaata actattgcaa aattatatct
     1561 taattaagaa aatattttta ttttaaagat aaaaaattct tcatcctgca atactttata
     1621 ttctatattg taattattca gaatattact tacaatatat aaacctaatc cattactatt
     1681 ttccttattt aaatcaaatt taacatcaaa tattttatcc atattcgaaa ttttattgtt
     1741 accgtatgaa ttctctatat ataaccaatc attaactatc ccaatattaa ttaccccatt
     1801 tacatcagta tattttaccg cattactaat caaattagat agaataatct ttaaagctgt
     1861 ttttcctata taaacattct cgtctaatat ataattattg acagttattt tcttttgatt
     1921 tgctaatatt tcatactttt ttaatatgtc ttctaatgta tcatttattt tcaaatattc
     1981 ttcatcatta ttcaaatttt ctatagaatg aactgataat atttgagaaa tattttttgt
     2041 taaactatca acgatttcaa tacattcatt aatataaata tctctttctt tatattttcc
     2101 aatattgtat ttcatgtttt ccagtattat tttaagacta gcaagagg
//
LOCUS       DAZULG010000206         2113 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000206 DAZULG010000000
VERSION     DAZULG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.213"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1002)
                     /gene="cysK"
                     /locus_tag="ACG9CY_002828"
     CDS             complement(76..1002)
                     /gene="cysK"
                     /locus_tag="ACG9CY_002828"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS4939272.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1142..2029)
                     /locus_tag="ACG9CY_002829"
     CDS             complement(1142..2029)
                     /locus_tag="ACG9CY_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4939273.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          584 a          472 c          356 g          701 t
ORIGIN      
        1 atcataaatt tttgtgtgtt cttttcatct ttaaaagttt tttacataaa cctatctgga
       61 gaaaaaccaa gtgccttata tctcttggta aagggctgta gataaataac gttcaccatt
      121 gtcgggaacc acagctaata ccttcttgcc ttttcctaat tcttttgcga cttttagcgc
      181 ggcagcaatt gcagcgccag atgagatgcc tactaaaatc ccttcttttc gcccaacttc
      241 tctagctgtt tccatcgctt cgtctccact aatagaaagg actttatcat acacagaagt
      301 atccagtgtt ttaggaacaa aacctgtacc gattccttgg attttgtgag gtcccgcttc
      361 tttcccttct aaaatagcag attctgctgg ttctaccccg attaattcga ttttcggata
      421 cacacgtttt aattctcggc cagcgccagt gatcgttcct cctgtaccga tacctgcaac
      481 aaacgcgtcc aagccattta ctccaaatgc ttgatgtatc tcaggaccag tcgtcttctc
      541 atgcacaaga ggatttgctt cattttcaaa ttgtaatggt aaaaagtagc cgttttcttt
      601 tgccaaacgt tctgcttctc tgattgatcc agaaatacca tccgcaccag gagtcaaaat
      661 caactctgcg ccgtaccctt tcatcaataa acgtctttcg atactcatcg tctcaggcat
      721 cacgatgatt actttatatc cttttgcaac tcctaccatc gacaatccga ttcctgtatt
      781 tccagaagta ggttcaatga tagtatcacc tggtttcagt agaccatcgt gttctgcttt
      841 ttctatcata cttaaagcaa tacgatcttt tacacttcct cctggattaa aaaactcaag
      901 ctttacaaaa acatctgctg aatcttctgg tacgatttta tttaatttga cgattggcgt
      961 tttgccaatc aattcggtga ctgagttata gatagctgtc atcttcaatt cctccttgtc
     1021 tttgctcttc ttacttatca taatacaatc tgttcattta tggcgagtga ttcgtctttg
     1081 actagcaaaa aaaagacaaa gatataccat atatggtaat ttctttgcct ttttatctaa
     1141 atcaaacgtt tagttcttga ccaactaata aagaataact ggacatagac atgccatttt
     1201 tcgccattag atcatcaaca gacatgccaa atttttgtgc aatatcccat agcgtatcac
     1261 ctgacacgac tgtatatgtc tgaccattat ttgtatttac atctgttgtt gaagatgatg
     1321 acgttgaagt cgtcgtagta gtcgttgttg ttgtacttgt ggttgttgta gccgaacctg
     1381 ttgatggcgt atcaaaacgg gttaaattat aagtattaat caatgagttc aatttggccc
     1441 cataatttgg atcggtagca taacgacctg tcaagtacgc tgttgcatca taaaaacttg
     1501 ttgtattgct tttccacacg ccagcataat gataatcgcc cgtggaaaaa ctggttgaac
     1561 gaagaacatt cgcatggtct tggaaggatt cccaataaga agaatatttt cggaatggct
     1621 cattcatcgt tacccattgt ccgtttacat attctttggt tgccatataa acgacctgtc
     1681 cgttatagct tcctttcaca ccgaatagat tataatttgg cgcactagct aatccggaag
     1741 tgccatatgc actttccaac aacgcttgag cgatcatgac tgaagcatac aagtcattac
     1801 ttgcagcaac ttctgacgca gaccagccga tttgatcgat aaacgcttga ggatcagcga
     1861 ctgtttgaga tccttgttca tctgcatgaa catgttgcgg taccgctgta accatcatcg
     1921 gcccaactaa aagactcgtt ccaattaaag gagcaatcgc ttttttattg atcaccattt
     1981 ttcttttagg tctatggtgc cgggcactgc gggtcttcag ctcttgcatt caaattcctc
     2041 ctcaaaataa aaaaacttaa taaaaagttc tcttcttaaa acaaacttaa ttaattaaat
     2101 tatactttta att
//
LOCUS       DAZULG010000207         2093 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000207 DAZULG010000000
VERSION     DAZULG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.214"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1528)
                     /gene="pglX"
                     /locus_tag="ACG9CY_002830"
     CDS             complement(<1..1528)
                     /gene="pglX"
                     /locus_tag="ACG9CY_002830"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFS4939274.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     A"
     gene            complement(1592..>2093)
                     /locus_tag="ACG9CY_002831"
     CDS             complement(1592..>2093)
                     /locus_tag="ACG9CY_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFS4939275.1"
                     /translation="ESQEILNKLTEEKQATFKLLEGKDFKDQFVPIVDQEFQELKAKI
                     EQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIEAANLIRETEKDEI
                     DSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAEINQKLKEQLQENT
                     EITIQF"
BASE COUNT          594 a          406 c          269 g          824 t
ORIGIN      
        1 ccgctacata tttatcaact agcatttcag ccacgtcaag aatgtgcaag tatttttctt
       61 gtatttcctt catcccataa agaatatcca tattcgtaaa ttcgttgaat tttgatatat
      121 actctcttac ttttcctaag tattctttag ttattgcctg attattttta tcgactaact
      181 taatgattga tccaagttct ttcccatttt caaattgatc aaaaattaac tctttgtatt
      241 cattccagcg ttcactatac ttatcattgt tcaattcact aatacgctct tgatactcgt
      301 ctgtcacttt aggagaatca ataaactctc ttaaattcgg cgatagactt tttttcaaaa
      361 aaagacgatc gtattgacgc cctttcatca taattgcaaa ataagacaat tgatacgctc
      421 ttttatcgat ttcaagacca tataaattgt attcaacgat tgcacgtgca gcatcacgct
      481 cagaatatcc taacgtaaga taaatttcca tcaatacatc gaaggcgtaa actaaaatat
      541 gcccagaccc cattgcgtta tcgataattt ttagttcttc tggttttaaa ttacttacag
      601 atgttgcttg ttcaaaagca tatttatctg gaattaagta tttcaactta tttcttaatt
      661 cactattggg ataatgatct aaccaatact ttcctaaact attgtcaacc atataacgaa
      721 cgacccaatc tgtggtaaat agttgagttg ctgccggaat atcttgtttt ttgacaatcc
      781 ctttataaat attcaccact tgattatgtg gctcactatt atagtactga tacaaccacc
      841 caataatctc tacctgtcca ccagcttgaa catagaaatc atcctctgga atacttaaaa
      901 gatcagcgat tacaccatct tttttaatat aagaagctgt aaacaaaagt tctgtataat
      961 cttctacttc ttcaaatagc tcaggtaagt aatcattcaa ttgattacag atttttataa
     1021 aaatcaattg atacagcttg tccattgctt ctgctgaatt atctgttttc caagttctga
     1081 cttgttgctg ttcattacta gataattctt caaagatatc ggcatcataa atatctgtaa
     1141 taatatctgg ctcaattttc ccacttattt cactagataa tacacgatac gtgtcaggta
     1201 aataatcatt tacctccata aaccgaatgg caatcaatcg gttaaaccac gtataagcaa
     1261 cctcttcgat taactcttga aaagcttctg aataagaagt gacagaagca cgttgtttta
     1321 gttctgcaat caatttgttt ctttgcgcta tttcttttcc ttgaattctt attcctgatt
     1381 tttcgatttc ttgtaaattg ctatcaattt tcttcacatc ttcaattttt gattcatcaa
     1441 ttcctaaacg ttttgcttta gtcattactt catcaattaa ttttctacgg gcattgaccg
     1501 cgaaattctt tatcgctttc ttatccattt ttctttccct tctatatgta acctttgaaa
     1561 agacaggcac ctttttatgc ctgtcttttc actagaattg aatcgtgatt tctgtatttt
     1621 cttgaagttg ttccttcagt ttttgattaa tttcagctgt taactcatca atatctgctg
     1681 ctgacttaat agttttaaca tgaacgttaa gtgttctcat tggtactgtt ttctgttgaa
     1741 tagtaggttt tactatcgct atttcttcag cttttgctgc agagtctatt tcatcttttt
     1801 cagtttcacg aatcaagttt gctgcttcaa tttgtttacg tgcttgttct tcttgacgtc
     1861 tgcggttttc ttcattatcg aaacgttgat taaagatcaa atactgttct gctacgttta
     1921 atgataagaa aacaagatca tctatagtat tcgcttgttc aatttttgct ttcagttctt
     1981 gaaattcttg gtcaactatt ggtacaaatt gatccttaaa atcttttcct tctaataatt
     2041 taaatgttgc ttgtttttct tccgttaatt tatttaatat ttcctgactt tcc
//
LOCUS       DAZULG010000208         2084 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000208 DAZULG010000000
VERSION     DAZULG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.215"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACG9CY_002832"
     CDS             complement(<1..426)
                     /locus_tag="ACG9CY_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS4939276.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACG9CY_002833"
     CDS             complement(535..1566)
                     /locus_tag="ACG9CY_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4939277.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          647 a          336 c          360 g          741 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaattata ttgagaatta atcctttttt atataagttg tttgcttatt
     2041 gatggattgg ataaattaaa ccatcacaac gagtggaaat tata
//
LOCUS       DAZULG010000209         2057 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000209 DAZULG010000000
VERSION     DAZULG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.216"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..>2057
                     /gene="acm"
                     /locus_tag="ACG9CY_002834"
     CDS             18..>2057
                     /gene="acm"
                     /locus_tag="ACG9CY_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS4939278.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQI"
BASE COUNT          808 a          333 c          407 g          509 t
ORIGIN      
        1 taggggagat agaaaaaatg aaaaagtgta gaaatattat tttatcaatg ttatttatta
       61 ttactaatat aacatcactg attcctgttc atgtctatgc ggatgcaggc agagatatca
      121 gcagtaatgt cacttcgttg accgtggacc caacgaatat tacagatgga ggaaacataa
      181 aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat tatctttgga
      241 tcaattggcc aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa atcccattaa
      301 tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa agattctgcg caggttgtgt
      361 ttaatgaaaa tattaaaaac ctagactcag tagaaggttg ggggcaattt gagatacagg
      421 ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc attcaccgtg acgagcggtg
      481 ataaaacagc tactgtaaat gtgacaaagc cagcttctgg ttcttcttct agcgtttttt
      541 attataaaac aggagacatg ctacctgaag atactaagca cattcgatgg tttttgaata
      601 ttaacaataa cggcacttat gttgaacaac cagttaaaat cagtgatgaa atccaaagtg
      661 gacaaagatt agatccaagt acgtttgaaa ttaatcaaat acacttaggt gaacagaagg
      721 tttatagagg agaagaggga atccaacaat ttttacaaga tttccccagc gctacattta
      781 atttttcagt aactgataat tacattgaaa taactatacc aaaaaacttt gtcaatctta
      841 ggaaaataat ggtgagttat aagacgatca tagaaaaccc agaacaaata aattttgaga
      901 atcattcgga agcttggttt aaagaattta ataaacctgc tgttgatgga gaaagtttca
      961 atcatacagt taaaaatatt tcagcttctg gtggagtcaa cggtacagtt agaggcgaat
     1021 tgaagatttt caaatatatc aatgacacag agattggtat cccaaatgtg actttcgaat
     1081 taagacgagc agatgaacag cctattcaag gacagtctag tatcctgttg acttcgaatg
     1141 aacagggaga agctagtatc aaaggcctgc aagtaggaga ctatgttgta aaagaaaaag
     1201 aagcacccaa ttggattgat tttgatccac taagttcaaa tgaactcaaa ttctccataa
     1261 acgaaaatga tacagaggga gtcagtttac ctatttataa caagaaaaag gttacgaaca
     1321 tcacagcaac taaaatttgg aacggtggaa ccactccaag accgagtatt tattttaaat
     1381 tattcagagc aagtacaaac aattgggaac cagttcctga tgcagaaaca aaacgattag
     1441 acaatggaat aacttcagtg acttgggaag atatccaaca gtatgatgat agtggaaacg
     1501 aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc ccttcaggtt
     1561 atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc atttcagtct
     1621 caggacaaaa aacatgggaa gacaatgaga accaagacgg caaacgaccg acgacgatca
     1681 cagtaaatct attagccgac ggtcaaccga tccaacacaa agaagtaagt gaaaaagatg
     1741 attggcgcta tcgtttcaca aatctgccaa aatacagaga cgggcaagaa atcatctata
     1801 ctgtaacgga agacaacgtg cccgaataca gtacgacgat tgacgggtat aacatcaaaa
     1861 acagttatac accggaaaaa acaaatatat ctataccagg tagccatata acaccgtgga
     1921 aaaattttcc agagaagaca gagagagaga aaattagaaa tctattctct caaaaattgc
     1981 aaatatttgc tcatacagga gaatccagcg aacaaagaag caattatatt agtaagagag
     2041 ctgtacctaa acagata
//
LOCUS       DAZULG010000210         2045 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000210 DAZULG010000000
VERSION     DAZULG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.217"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            658..990
                     /gene="traE"
                     /locus_tag="ACG9CY_002835"
     CDS             658..990
                     /gene="traE"
                     /locus_tag="ACG9CY_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS4939279.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1609..1950
                     /locus_tag="ACG9CY_002836"
     CDS             1609..1950
                     /locus_tag="ACG9CY_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS4939280.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          713 a          271 c          431 g          630 t
ORIGIN      
        1 gtgttaaaag aaataattaa tttcaaatat agaaaaggga acgtatgttt gcatactcct
       61 tttttttgag ttataataat acgcgaagag agataatttc attatttaaa caagctaaaa
      121 atcttgatat tacttctttt caggcaatac gaatattatt cgcttacatt ttttgtataa
      181 atttaattgt ctgctatgct tgtgatgaat tgattttcgt tttgcacttt tggatgataa
      241 atttggacaa ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa ggctggtaac
      301 cttcgtcagc gttcatacac ggcattaaga tttggccctt aatgcgttaa cctttcagtg
      361 ttacggtatt acccgcaatg gatttagtat aacagatatt caaaagtgat accacttttt
      421 ttggctgtgt taaggttaaa aatacgtgat attacgatga atctatgcct aagcataatg
      481 ttataaaaat gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta gagggaacgc
      541 atgtttggat gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt ctctattttt
      601 gaaaaataac aagaaaaact tgttaaaaat atttaaagaa attgggtggt ttattttatg
      661 tcatatacag gaattttgag tctcaaagat atttgtcact acggtaaacg gtgtaccgct
      721 acagagaaaa tcacgaagaa actatcaact ggccaaagta aaaccgttgt gcagtgtaag
      781 aaatatataa tccagaaaga caaagtctca gaggaaatga tttattacat tgggaaacaa
      841 aagcaaataa ttttaaaaga tcctatcccc ttaaaagagc tatatccaac aatcaaacat
      901 gtttatgacc aaaacggtgt actgattggt cgaagaaaaa atggagtctt gcgttgtacc
      961 gcaaaaggga tggggcgttt gatcggctga attgatcatt ctcataactt gtattttatc
     1021 gtttttcgct tgcttgaaaa tattgtcgag acttctaaag gaacttagag tcactagaaa
     1081 aataaggagt agacttgatc tatcaacaat tcccttcggg gagtgacggg gtaggtttta
     1141 acgattggta acgtaaaaaa ttggtcaatc gagtaaatgc gcttgtttgg atagtggtga
     1201 aatgaatttg tgtaaataat aagcgcagag taaacaagag ggaatttaga agttagacaa
     1261 gttagaatag cgtctagatt aatcatgagc agctactaag ggatctagac tttgacgcaa
     1321 actcatggaa taaggagtag gtcttctcgt aatggggagg acgcacaaga aattgtgtcg
     1381 tgtgctgcag tacatgtaga tacttgcgag gacgttgatt attggatcaa tcgagcattt
     1441 accgctgtct actagggttt ctcgtttcgg acgccataaa cgagtcgaaa ttttttatgt
     1501 atttttaagt aaacgaaaaa cgataaaata tgaacaagtc ctgaataaag tgaaaaaatt
     1561 gatataaaac atagagaaaa tgaaatttac gtaatggagg aatgacaaat gaaaaattat
     1621 gatccgaata tacgattggg cacacatact attaaggttt ctttccaacg ttgggattac
     1681 aaaggatttg tgacgtttag aaggggcgga aattgtaaag gattagatgt tttagcatta
     1741 gacgaggatg atttatacga tcaaacattg actgataatc caattggatt cggcttgcta
     1801 cctgaagatg atgaaggtaa tgaatggttt aaaatgacgc ttatgaatga taatggagac
     1861 gaattatctg ttgaagatac ttggtcatat ttgagtgatt atattgtcag ctttgaaatc
     1921 attgaatttg tagcggataa agaagaataa aaactggagg aataaatttt acagataaag
     1981 gcttagaagg agtcacgacg attaataaaa aggtagtatt accttaggca ttgagtaagg
     2041 aattt
//
LOCUS       DAZULG010000211         2027 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000211 DAZULG010000000
VERSION     DAZULG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.218"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(372..1958)
                     /locus_tag="ACG9CY_002837"
     CDS             complement(372..1958)
                     /locus_tag="ACG9CY_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS4939281.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT          663 a          417 c          377 g          570 t
ORIGIN      
        1 ttgattttgt cgctttcgtt tatgagctat ttttcttaca ccttttactt gactaatcgt
       61 aggaaaggga actgtgacaa aagtcttgtc gcagttcctt tttccgaata aacggtgctc
      121 aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacataaaaa gctgttttta
      181 cttggctgtt cttattactt gatgcaaggc agctttttca caacctctga tacacggtgt
      241 tccagaagta acttcttcgg aaataagccg aaatttcaca aaaatttgaa aagcaatttt
      301 cttaaattcc ttcttatttc ttgaagctgc ccacttctga cacaacctcc tttgtgtaaa
      361 agaatcagtt tctactcgat ttcttctatc gtttcctgca aacgctgttc atattttttg
      421 tttcctttat ataaatcacc aatcgtccat gccaacagga gaaggacaag aacaatgatt
      481 acataagcga tttgatcagc caatgctagt tgagctgagg tggcttgctc gccgaagaaa
      541 ctttcgattt gacctggtaa tcctcgtaaa ttcaagtaag tcagactaat cacagagaac
      601 cagccaagta gttttacaac aaatgaattt ttgaatcggc tccccatctc cgcttggcta
      661 ttggtcatca tcagtaaagg aatcatcgag aatggcaatg caaaagccag aaaaacttgg
      721 gattcattca tcaagttatt caaagcggta tgttcttcga tcgttccttt tgtacttgta
      781 aacaaaacac aaatcaatac aggaatcaca gaaagcaatc gtgtgactag tcttcttagc
      841 caaattggga tacgcatatg gatgaagcct tccatgatga cttgaccggt caacgtacca
      901 gtaatagtag agttctggcc agaagcaaga agtgctacag caaataaagt agacaagata
      961 ccagattttg ctacagcaat caagatacca ttgctcatag ctgatggatc agataaagct
     1021 tcatacagac caaaaaagga aggatcttgg acagaaccgg ttttgaatac agccacaccc
     1081 atgatcaata gcagtgcatt gactagaaaa gccattgaca actggatatt agaatcccaa
     1141 gtagaaaagc gtaccgcttg tgctacatct tcttcgtctt ggtgatcgat cttccgagtt
     1201 tgagaaactg ctgaatgaag atataagtta tgcggcatga ctgttgcacc aataatccct
     1261 aaagctccag tcagcggagt ttcgccgccg atgaccggtg agtctgagaa tgtgtgactg
     1321 ttagggataa atccggcaaa caaactgtgc caatcagggt tagacaagcc aacttgatag
     1381 acgaaaacga ataaaatgac gaaaatcaag cagacaacca aagcttcgat tttacgaaac
     1441 ccgacttttg tcagcaataa aaggacgaaa acatctaaca cagtgataaa tacggcaatt
     1501 accaatggaa tatcaaaaag caggtagagg gcgattgctg caccaatgac ttctgcgatg
     1561 tctgttgcca taatggctaa ttcagtcaag atccataaaa tgacacccaa tgtcttactt
     1621 gtccgtgctc ggattgcttg ggccaaatcc atttgggtca cgattcctaa ttttgctgcc
     1681 atatattgta aaagcatagc aatcagacta gacatcaaaa taactgacat caataaatat
     1741 tggaaatttt gtccaccagt gatcgaagtg gaccagttac ctggatccat ataaccaact
     1801 gctactaatg cacctggtcc agagtaagca agaagtgttt taaaaaaact ttttcccttg
     1861 ggtacatcca ctgttccatt tacttcagct aaagataaac catttgtatg ttcaataagt
     1921 cgatgcttat gaaattgttt gttggattga ttattcaaga aaaaaccacc tttcctattt
     1981 atctacgatt acattctaca cctcagaaag aaaaaattaa agaaaaa
//
LOCUS       DAZULG010000212         2022 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000212 DAZULG010000000
VERSION     DAZULG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.219"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            685..1872
                     /locus_tag="ACG9CY_002838"
     CDS             685..1872
                     /locus_tag="ACG9CY_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS4939282.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          644 a          335 c          394 g          649 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct ttttacgatt acgtccatat aattgtgtaa
      601 aagtgaagca tcaaaaaagc cattgatctc agttaggagt acaatgtttt ttgcagaaaa
      661 catactcgaa taggactgat atcaatgact caagtacatt ttacttttga aagcgaagaa
      721 attcaagcaa ttataaacga aagcggtgcg aatgataccg caaaaacact tatgactatc
      781 atgtttaatc agctgatgga agaacaacga aatcaatata ttcaggcaaa tgcctatgaa
      841 cgttcagaag aacggcaatc acaaagaaat ggttactacg atcgttcgtt tacaactcga
      901 attggtacgt tagaacttca tgttccacga accagagacg gtaagtttag tcctactatc
      961 tttgaaagat atcagcgaag cgaaaaagct ttgatcgctg ccatgattga aatggtcatt
     1021 tctggtgtct ccactcgcaa agtaactaaa acagttgaac tgctaacgga tggtgcgact
     1081 gtttctaaat cattcgtttc aaatctgatg aaacagttgg atccatttgt ttttgagtgg
     1141 agaaaccgga gcctagaagg ctcggaatat ccatttttca tgtgtgatgc cctgtatatg
     1201 aaagtaaggg aaaatcatcg tattgtctca aaaggtgtat atattggtat cggcattgat
     1261 tctgatggac gacgtacgat tcttggtttt gatgttcaag acggcgaatc ggaagataat
     1321 tgggataccg tttttcaatc gttcgttcaa cgaggtttgt tcggtgtaaa actggtcatt
     1381 tctgatgcac acaaagggtt agttaaggct gttcgcaaga actttttagg tgcgagctgg
     1441 caaagatgcc aagcccactt tttgagaaat atttttgata agctgccaaa gaaagtttct
     1501 tctgatgtaa aagacgagtt gaagagtatc tttaaagcct ctgaacttga attgacacgt
     1561 gaaagaaaag agcacttctt ggaaaaatat ggttgtgatt caaaattaag tgctgcttgc
     1621 gacattttag aaaacggctt tgaagatgct attcaaattt tgtcttttcc tgaaaatatc
     1681 cgccgaagga ttcgtacaac caatgtgttg gaacggttga acgaagagat tcgccggaga
     1741 gaacgagtga ttcggatttt tccaaatatc aattccatca ctcggataat tggaacactt
     1801 ttgatggaaa aagacactga gtggctggct tctccacgaa aatatttaga atttaattcg
     1861 aataacattt agcaccatga acctgtgcga gtctcttctt tctgaggaga gacatcaaga
     1921 gtcgctgcgc tactgctcct gacctctctc ctcagaaaga aaggcatggc agttaggttc
     1981 acaacgcaaa acattgtgct tttgatgagg ttgaactttt ac
//
LOCUS       DAZULG010000213         1995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000213 DAZULG010000000
VERSION     DAZULG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.220"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(367..1158)
                     /gene="msrA"
                     /locus_tag="ACG9CY_002839"
     CDS             complement(367..1158)
                     /gene="msrA"
                     /locus_tag="ACG9CY_002839"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4939283.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1349..1984)
                     /locus_tag="ACG9CY_002840"
     CDS             complement(1349..1984)
                     /locus_tag="ACG9CY_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4939284.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
BASE COUNT          644 a          415 c          315 g          621 t
ORIGIN      
        1 tatagttatt gtttgcgctt ttatacggaa tttagttcat cattaatgtg gaattcgatt
       61 aggtgtctat taattatcaa gatgattctc tacaaaattc catagaaaaa tcaagattct
      121 acaattcgaa ttctctttca catggaaata aaggctagta aaaaaacagg agttgtgaca
      181 aaagacttgt catacctcct gtttccttgc gacttgaagc tgaatgcttt gcgccacaac
      241 cttttcctcg gtgttctcaa agcaactcct cgaaataatt cataactgtc caaaaatatg
      301 aaaagctatt tttgtcagtt gctcattatt ttgtcggagc tgaacgcttt gcgccacaac
      361 cttcctctat ctatgtcgct gaaaaaataa atgaagctga tgcgaatgct tgatcttttt
      421 atatctttta ggttgcttct gataaaattg ttgatgatga ttttcagctg gccaaaaagt
      481 ctttgcttgg cgaatctctg taacgatagg acggctaaat cggttagatt gaatcagttg
      541 ttctcttgat ttttcagcca attgcctttg cttttcatta acaacaaata gaattgaacg
      601 atattgtttc ccccgatctt gaaattgacc aaaggcatct gtagggtctg aaatttgcca
      661 ataaatctcc aacagctctt cataggttat ttgacgtgta tcaaatatga tctcaacagc
      721 ttcaacatgc ccagtatatc caccactgac ttgatcgtaa tttggttttt tggtctctcc
      781 acctgtataa ccagataaca ctgagattac acctggtttt gtttcaaaag gctcaaccat
      841 acaccagaaa cagccaccgg caaaaatcgc ccgttcttgg tataagggtg aagttatctt
      901 atgtttggca taatcataag cctttgcact cgctgactcc cctgtgattc ttaaataaaa
      961 atccatcgca tctggtgtca aattatctcg tagtgctagc ggacgcaagt tgacctctag
     1021 tttttctatc acctgcttta tcgacatttt tgctaggtct tcttttgcct cttcaaattg
     1081 tgttctttcc cattctcttg tatttcgatt taaaataata ttataaaggt ctatttccat
     1141 ctgctccaca tctaacatga agtcaccctc tttcaagttt tcttcttatt cattcaaaaa
     1201 atgactacct gaaatttagc acaacgcata acaagttaag actaacttgc ttttcgctct
     1261 cctcatctct attcttagtc gaacacatcc acacgaatgg ttacccatag agaaattctt
     1321 ttacttaatc agcttcacac tgctttttct actttcgtag ttgaaaaaaa cttaaacaaa
     1381 tcatcggcaa atagttaaag aaaaaactta agcgttgaaa gaagcccaat tgcttaaaga
     1441 aggcaaactg actaatcgac ggaatccgtg ctaatccgta aagacctgca attagtaaac
     1501 taataactgt taaaacaaga taaaaatggc taagcgtacc ttgaccactt tgacgataaa
     1561 gcagaacaag taacaatggg aaaagtagaa agcctgcata gcctagccca gaacctatat
     1621 tatggatcca agtcgataga ttccaagagc tttcattcgt attgatactg aataacccag
     1681 taaaaataca atcacctatc ccatataaac caactgttgc aacagcaagt attgcaagtg
     1741 tttttgatgt tccaataaat aggtgataca acgcaggcaa cgaaagaaca aaaaaaagtc
     1801 cagatacaac tgaccagacc aagaaagcac gtctgactgg actatctacg tcaccaaaca
     1861 cactaattac ggtagtcatt tgattcagtt tgggataaaa aaggccaagg atatacggtg
     1921 tcgataaatc actaatcaca cctaataata aaaaataaaa accatatttt tgcaaaaaaa
     1981 tcattttctt ctcct
//
LOCUS       DAZULG010000214         1990 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000214 DAZULG010000000
VERSION     DAZULG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.221"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1657)
                     /locus_tag="ACG9CY_002841"
     CDS             complement(365..1657)
                     /locus_tag="ACG9CY_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4939285.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          639 a          388 c          323 g          640 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa
//
LOCUS       DAZULG010000215         1979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000215 DAZULG010000000
VERSION     DAZULG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.222"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..367
                     /locus_tag="ACG9CY_002842"
     CDS             <1..367
                     /locus_tag="ACG9CY_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS4939286.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..749
                     /locus_tag="ACG9CY_002843"
                     /pseudo
     CDS             567..749
                     /locus_tag="ACG9CY_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1330..>1979
                     /locus_tag="ACG9CY_002844"
     CDS             1330..>1979
                     /locus_tag="ACG9CY_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4939287.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVDDK
                     VHPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQ
                     LFDQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIA
                     CDILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          657 a          305 c          461 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggtttaact
      661 tggggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcgttaaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacga tcgtgttaga ttgtgaaaac cgatagctga atgaaataaa cagcgggtgc
     1021 gccctttcca tttcggcagg aggaggctca aggggttcgg ggaatgaaat tccctgaggt
     1081 ctttttattt ttttaggagg agtgctataa tcaaagaaaa cgagaaaaaa gagcaaaata
     1141 aaagccgagc agtgcgctaa cactctcgac tatttacgga aactacttca cctagctccg
     1201 taactcaggt tcattatacg aatattttta ggtttcttca agctagagat ttttggatat
     1261 gaatttttag ggttacgtga actctggaaa ttctttccaa aagtctcttt tttcgtctgg
     1321 aggacgaaaa tggcaaatga aaatcagttg agtgaaattc aatcaaaaat tgaaggtcga
     1381 ggggcagaaa aaagaacaac gataaatcag ctcgaaaaac ttaaccgtat ttctaacagg
     1441 cggttaagtg ttgacgataa ggtacacccc ctaaaattaa gttggtcaat cgatcgaatt
     1501 acaatcgtcg gaaaactgaa tgataccata gcttattgga cgcaaaatgg agttgtgttt
     1561 gtcgattttg agcaattaat gcgtttgaat gaaaatcaag ggtatttgaa gtcggttggt
     1621 acaaatggtt ggcaattatt tgaccaatat ggagaagata tcgcctatat cgaaatgtta
     1681 aaatttcacc ctgacaaagg tcgtattgat tttaatccaa ataaaattgg cgagttttta
     1741 aaggggtcga tgaaacaatt cattcatgat ttgtttctgg atgcgcattt ctcacgtgca
     1801 gatattgctt gcgatattct cggtgttcct gatgatttta ttacccagta tcgaatcgtt
     1861 gaccctgtgt cttttaagcc aatttatggc cgttctggca aattagaaac agcgtactgg
     1921 ggtagtcgtg ccagcgagcg acaagtgcgg atgtacaaca aaaagcttga acaggaacg
//
LOCUS       DAZULG010000216         1978 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000216 DAZULG010000000
VERSION     DAZULG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.223"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..575
                     /locus_tag="ACG9CY_002845"
     CDS             93..575
                     /locus_tag="ACG9CY_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939288.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            772..1662
                     /locus_tag="ACG9CY_002846"
     CDS             772..1662
                     /locus_tag="ACG9CY_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFS4939289.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          648 a          337 c          407 g          586 t
ORIGIN      
        1 attatctcta aaaaaaaagt ttttccccct aattttttta gatagaaaat tgtatagtat
       61 gatgagagaa aaagtagttt gggggaacgt taatgagaag aaaagcagta atcgatcggc
      121 tttatttatt tttttggttt tttcctatcg ttttgctacc ttttacatgg gtaaatgctg
      181 ttgtacaagg tgcggagata ttaggcctga tccaaggaag cggatttgtg gtttctagga
      241 attttccagc tttgattgtt ttctatttag tgataagtgt aggtctctta ttaccaaaat
      301 ctctgatcaa tcactacaat tggcagctgc tttacttttc ttgcttatta gtatttacat
      361 ctacgttttc tttgctgcct cgcttgatta tcggccctaa tggtttgagc gagagtggat
      421 tgaaggattt agcttattcc tccatgtatt tcttgacgat caaaccatgg ttctatttag
      481 taattgcctc atttattctt gcaacatttt tttacttctg gacagaaatc aggataaaaa
      541 gtgaacgatc tgtatctata aaaaataact attgatggtg gatcatcagt aaaaaaacat
      601 atgagctgcg taaaatgcat gtttctcata tgcttttttt attattatgt aaagaatgcg
      661 aaaaacgttt acatgatttt tacataactt aactatattt ttacagcaac catacgaggg
      721 atttacagtc gactgataaa ctttagttgt aaataagaaa ggagcaataa aatgaaaaaa
      781 gtattgttta ctctaggtat gatgggaatt cttttagccg gatgcggctc aggatcagga
      841 aatacagatg gctctgccac acaaaataat tcaagtgact caaatgaaca agtaaaaatc
      901 gttgcggtag gttcgacagc attacagccg ttagtcgatg cagcacaaga aagctttgta
      961 caagaaaatc caaattatca aatctctgta caaggaggag gaagcggaac tggtttgagc
     1021 caagtcgaag ctggagcagt tactatcgga aactctgatg tatttgcaga agaaagagac
     1081 ggagttgatg cttcaaaatt agtggatcat aaagtagcag tggtcgggat ggcaccaatc
     1141 gtcaataaag atacagatgt caaagatatc accaaacagg aattgattga tatctttaca
     1201 ggaaaaatca ctaactggaa ggaagttggc gggaaagatc aaaaaatcaa cgtcgtgaac
     1261 cgtgcaaacg gcagcggtac ccgcgcgaca ttcgaaaaat ggggactgga tggtgctacc
     1321 cctgtgcagt cccaagaaca agattcatca ggaacagttc ggcagctggt aagtcaaaca
     1381 ccaggagcaa tcagctattt agctttctca tatctcgatg attccacaca agcactaagt
     1441 atcgacggtg tagaaccaaa agaagagaat gtagcagata acagctgggg aatctggtca
     1501 tatgaacata tgtacacgaa cggaaaacct tcccctgaag tccaaaaatt cttagactac
     1561 atgatgacag aagaaatcca agaaggacct gtcaaagaat tgggctattt accaatcaca
     1621 atgatggaag tagaacgtga tcacgaagga aatatcaaat aactagcaga taaacatgcc
     1681 acggtagtcg ttactattaa ggaaaaatca actatgtttc cccacatata catttttcct
     1741 ttaattgtga aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
     1801 ctgattgaac accgtttatt cgaaaaaaag agctacgaca tagaactgtc gtaacttttt
     1861 ttggctcttc gtcaaatcgt gttggtgaag acaaatttga ataaaactta gaccgatatg
     1921 cggcgcgttc tgtgcgccgc atattttttt agttttcaaa gatcagacct tgttgtaa
//
LOCUS       DAZULG010000217         1936 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000217 DAZULG010000000
VERSION     DAZULG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.224"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..564
                     /locus_tag="ACG9CY_002847"
     CDS             94..564
                     /locus_tag="ACG9CY_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS4939290.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            786..1889
                     /locus_tag="ACG9CY_002848"
     CDS             786..1889
                     /locus_tag="ACG9CY_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS4939291.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          651 a          319 c          451 g          515 t
ORIGIN      
        1 ctgaagaata aatcacgtga tcgtgacgat aaaatacact tacgtcaggt gataaaaaat
       61 acataagtag aaaactaaga ggaggaacag aaaatgaaag aagttcaatt gatcagaaaa
      121 agcgaactat cagaaggtgg ttgcaatgca tgcggcgtag tagaagcaac aagctataca
      181 ttaaagcttg gtgccaacaa agcgataatc tctgagttga cagtaggcgg tcttgtggac
      241 agtctggcgc ttgctgaagg ttttattgga gaagatattt atgagatgtt ttcagaaatc
      301 cgtcagttaa aaaaagggga aaactgcata gaggttcatc atgagagccc gaatgtgaga
      361 ttcaaacgtg gtgataatga gatgatcttc aataatcatg tgtccgatca caccgagcta
      421 tatggaatcg tgaatcaaat actaactgag ctttttggat tgggacctta tgcgttcaag
      481 gaagaaaacg gtaatccaaa attaaacgaa gaatggcaag aaacgattga aatacaaaga
      541 aacaacccgc acttatttca ataggcatcc acagtatttc gtaaaaggga ctttgacacg
      601 acttttgtca aagtcccatc gttttttatc tatttgtgtt ctaaacattt ctttcagagt
      661 agtaacaact ggaatgactg aaatgtaaac agattatttg cctgtatcaa tacggaagag
      721 taatataaag tgtgtaaacg ctatcatatt attcattcaa ttttttatag agggaaggca
      781 ttgaaatgga aaaaacgtat caagcaggta caaatgaagg aatagtggat tttatcaata
      841 tggaagattt agaaatagcc gcttcacaag tgattccagc tggtggttat ggttacatca
      901 gcagtggtgc tggcgactta tttacttatc aagaaaatga acgggcattc aatcatcagc
      961 tgattattcc tcatgtatta aaagatgtag aattacccga tacaactaca cattttgaca
     1021 aagaaacgct gactgcacca atcatcatgg caccagttgc tgctcatggc ttagctcatg
     1081 tgaaggcgga gaaagcttct gcaaaaggtg tagcagattt tgggacgatc tatacagcaa
     1141 gttcttacgc ttcttgtaca ttagaagaga taagagaagc aggcggggag aaagcaccgc
     1201 aatggtttca attttatatg agcaaagatg atgggatcaa tttggatatt cttgaagtgg
     1261 ccaagcgtaa tggagcaaaa gcaatcgtat tgacagcgga tgcaacagtt ggcggaaacc
     1321 gtgaaacaga ccgcagaaat gggtttacct tcccactgcc aatgccgatc gttcaggctt
     1381 atcaatcagg agtaggccaa acgatggatg ctgtgtacaa atcttccaaa cagaagctta
     1441 gtccaaaaga tgtagaattt attgctgctc attctgattt gccagtctat gtgaaaggtg
     1501 tccagtcaga agaagatgtc tatcgttcat tagaatctgg tgctggcgga atctgggtat
     1561 ctaatcatgg tggccgtcaa ctagatggtg gtccagcagc gtttgattcg ttgcaatatg
     1621 tggcagaagc agtagataaa cgtgtaccaa tcgtgtttga cagcggtgta cgacgcggac
     1681 agcatgtttt caaagcaatc gcatcaggtg cagatcttgt tgctattggc agacccgtta
     1741 tttatggatt gtctttagga ggaagtacag gagtccatca agtgtttgat ttctttaaaa
     1801 cagaattgga aatggtcatg caattagctg gaacacaaac agtagaagat atcaaaaaaa
     1861 taaaattgcg tgagaatcgt tttatttaag aaacaaatga cgaaaaaaat aggagtgcgg
     1921 cagatgatgc cgtact
//
LOCUS       DAZULG010000218         1932 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000218 DAZULG010000000
VERSION     DAZULG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.225"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..539
                     /locus_tag="ACG9CY_002849"
     CDS             <1..539
                     /locus_tag="ACG9CY_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFS4939292.1"
                     /translation="VFIKPLNGSSSVNTFIVNNEKELLFFSEYIDNPLIQEYILGEEY
                     TIDVCLNFDSTPVSIVPRKRIATRGGEILKGQIIKDRELIETTKKMLEKLNAIGHITI
                     QCIKNEKGIFYLEINARYGGGAPISIKSGANSPEYIIKMIDSKKNLRYRENYLDKMIA
                     LRYDQAIYLDEKGNVYND"
     gene            532..1227
                     /locus_tag="ACG9CY_002850"
     CDS             532..1227
                     /locus_tag="ACG9CY_002850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4939293.1"
                     /translation="MIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIFPATFDEI
                     YRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLENADTLLTE
                     LRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPISYLLLLE
                     KFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFYQADKKIR
                     SLWEIKNFLGVIS"
     gene            1224..>1932
                     /locus_tag="ACG9CY_002851"
     CDS             1224..>1932
                     /locus_tag="ACG9CY_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFS4939294.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMS"
BASE COUNT          733 a          237 c          380 g          582 t
ORIGIN      
        1 ctgtatttat aaaacctctg aatggtagtt catctgttaa cacatttatt gtaaataatg
       61 aaaaagaatt gttgtttttt agtgaatata tagataatcc tcttattcaa gagtatattt
      121 taggtgaaga atatactata gatgtttgct taaactttga ttcgacacca gtaagtattg
      181 ttcctagaaa aagaatagca actagaggtg gggaaatact gaagggtcag attattaaag
      241 atagagaact aattgagact acgaaaaaga tgttggaaaa attaaatgca atcggacata
      301 ttactattca atgtataaaa aacgaaaagg gaatatttta tttagaaata aatgcaagat
      361 acggtggagg agcccccatt agcattaaat caggagctaa ttcccctgaa tatattataa
      421 agatgataga ttcaaaaaaa aatttgagat acagagaaaa ttatttagat aaaatgatag
      481 cactgagata tgatcaagcg atttatttag atgaaaaagg aaatgtatat aatgattaaa
      541 gcggtgatct ttgatttaga tgacactctt gtgtctgaat atgattatgt aaaaagtgga
      601 tacaaatgtg tagctagaag attagcagtg caaaaaattt tcccggctac ttttgacgaa
      661 atatatagag tccttttaga tctttttgct gaagatagta aaaatgtatt taatcgtcta
      721 tatgactatt ttgaaattcc ttatagtaaa aaagaaatta aagaactggt aaatgtatat
      781 cgagaacaca aaccagacat aaatttttta gaaaatgcag atactttatt aacggaatta
      841 agaagtagag gatacaagct ggggattatt acagatgggt atgctataac ccaacgaaac
      901 aaattggatg ctttgaaagt agactatttg gtagattata tttgtattac tgacgaatta
      961 ggccgtgaat tgtggaaacc acaccctatc tcatatcttt tattgttaga aaagtttaat
     1021 ttaaaggcac aagagtgtat atatattggc gataatgagt tgaaagattt tataactgcc
     1081 aaatcaatag gaatgcaaac aattaaaata gaaagagtaa atggtattta cagagaaagc
     1141 agtttgaagt tcaaaagatt ttaccaagct gataaaaaaa tacgttcgtt atgggagata
     1201 aaaaattttt tgggagtgat ttcatgaaaa gtaaaagaat tctattagct tctccacata
     1261 tgagtgaaga aggatatgaa aaagagtatg taaaagaagc atttgataca aattgggttg
     1321 ctcctttagg tgcgaatgtg aatggatttg agaaagaatt agcagaatac gtgggagcta
     1381 gtcatgctgc cgcattatct tctggaacag cagctataca tttagctttg aaagcggctg
     1441 gggtcggtaa agatgacata gtgatttgtc agtccttgac gttctcagct actgctaatc
     1501 caattctcta tcaaggagct atacctgtat ttgttgatag tgattacgaa acttggaata
     1561 tgaaacctga acatttgcga acagcactag aaaaatatcc taacacaaag gctgtattag
     1621 tagtacattt gtacggttta tcagctaatt tagacgaaat tactaaaata tgcaaagaat
     1681 acgatgttac tctaattgaa gatgctgcag aatcattagg aacaacatat aaaggaaaat
     1741 atactggtac atttggcgac tatggaattt attcttttaa tggaaataag ataatcacaa
     1801 cttcaggtgg cggaatgctt gtatccaatg acaaagaaaa ggtggaaaaa gtgagatttt
     1861 gggctacgca atctcgtgag aaggcacgcc attatcaaca caatgaaatt ggctataatt
     1921 atcgaatgag ca
//
LOCUS       DAZULG010000219         1880 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000219 DAZULG010000000
VERSION     DAZULG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.226"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACG9CY_002852"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACG9CY_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACG9CY_002853"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACG9CY_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          657 a          265 c          248 g          710 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt
//
LOCUS       DAZULG010000220         1847 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000220 DAZULG010000000
VERSION     DAZULG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.227"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..>1847
                     /gene="ltrA"
                     /locus_tag="ACG9CY_002854"
     CDS             700..>1847
                     /gene="ltrA"
                     /locus_tag="ACG9CY_002854"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4939295.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
BASE COUNT          730 a          281 c          384 g          452 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaaga
//
LOCUS       DAZULG010000221         1842 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000221 DAZULG010000000
VERSION     DAZULG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.228"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="ACG9CY_002855"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="ACG9CY_002855"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..279
                     /locus_tag="ACG9CY_002856"
     tRNA            207..279
                     /locus_tag="ACG9CY_002856"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Val,seq:tac)
     gene            283..355
                     /locus_tag="ACG9CY_002857"
     tRNA            283..355
                     /locus_tag="ACG9CY_002857"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Lys,seq:ttt)
     gene            363..444
                     /locus_tag="ACG9CY_002858"
     tRNA            363..444
                     /locus_tag="ACG9CY_002858"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:397..399,aa:Leu,seq:tag)
     gene            462..534
                     /locus_tag="ACG9CY_002859"
     tRNA            462..534
                     /locus_tag="ACG9CY_002859"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:495..497,aa:Thr,seq:tgt)
     gene            546..617
                     /locus_tag="ACG9CY_002860"
     tRNA            546..617
                     /locus_tag="ACG9CY_002860"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Gly,seq:gcc)
     gene            634..719
                     /locus_tag="ACG9CY_002861"
     tRNA            634..719
                     /locus_tag="ACG9CY_002861"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:668..670,aa:Leu,seq:taa)
     gene            730..803
                     /locus_tag="ACG9CY_002862"
     tRNA            730..803
                     /locus_tag="ACG9CY_002862"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:764..766,aa:Arg,seq:acg)
     gene            813..886
                     /locus_tag="ACG9CY_002863"
     tRNA            813..886
                     /locus_tag="ACG9CY_002863"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:847..849,aa:Pro,seq:tgg)
     gene            906..978
                     /locus_tag="ACG9CY_002864"
     tRNA            906..978
                     /locus_tag="ACG9CY_002864"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:939..941,aa:Ala,seq:tgc)
     gene            1006..1079
                     /locus_tag="ACG9CY_002865"
     tRNA            1006..1079
                     /locus_tag="ACG9CY_002865"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1040..1042,aa:Met,seq:cat)
     gene            1093..1166
                     /locus_tag="ACG9CY_002866"
     tRNA            1093..1166
                     /locus_tag="ACG9CY_002866"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1127..1129,aa:Ile,seq:cat)
     gene            1190..1279
                     /locus_tag="ACG9CY_002867"
     tRNA            1190..1279
                     /locus_tag="ACG9CY_002867"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1226..1228,aa:Ser,seq:tga)
     gene            1292..1365
                     /locus_tag="ACG9CY_002868"
     tRNA            1292..1365
                     /locus_tag="ACG9CY_002868"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Met,seq:cat)
     gene            1384..1459
                     /locus_tag="ACG9CY_002869"
     tRNA            1384..1459
                     /locus_tag="ACG9CY_002869"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1417..1419,aa:Asp,seq:gtc)
     gene            1461..1531
                     /locus_tag="ACG9CY_002870"
     tRNA            1461..1531
                     /locus_tag="ACG9CY_002870"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1493..1495,aa:Gly,seq:tcc)
     gene            1545..1618
                     /locus_tag="ACG9CY_002871"
     tRNA            1545..1618
                     /locus_tag="ACG9CY_002871"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1579..1581,aa:Ile,seq:gat)
     gene            1643..1730
                     /locus_tag="ACG9CY_002872"
     tRNA            1643..1730
                     /locus_tag="ACG9CY_002872"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1677..1679,aa:Ser,seq:gct)
     gene            1752..1823
                     /locus_tag="ACG9CY_002873"
     tRNA            1752..1823
                     /locus_tag="ACG9CY_002873"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1785..1787,aa:Glu,seq:ttc)
BASE COUNT          420 a          431 c          507 g          484 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgcttaagtt atccatggag gtttagctca gctgggagag catctgcctt
      241 acaagcagag ggtcagcggt tcgatcccgt taacctccat atgagccgtt agctcagttg
      301 gtagagcatc tgacttttaa tcagagggtc acaggttcga gccctgtacg gctcatattt
      361 ttgcgggtgt ggcggaattg gcagacgcac tagatttagg atctagcgcc ttacggcgtg
      421 ggggttcaag tcccttcacc cgcataattt aagttttaca tgccggctta gctcagttgg
      481 tagagcatct gatttgtaat cagagggtcg agggttcaag tcctttagcc ggcatcttta
      541 aacacgcgga aatagctcag tggtagagca ccaccttgcc aaggtggggg tcgcgggttc
      601 gaaccccgtt ttccgctttg ccaaagcaat cgcgccgggg tggcggaact ggcagacgca
      661 caggacttaa aatcctgcgg tgagtgatca ccgtaccggt tcgattccgg tcctcggcat
      721 ttcaaatatg cgcccatagc tcaactggat agagtgtttg actacggatc aaaaggttag
      781 gggttcgact cctcttgggc gcgttaatta aacgggaagt agctcagctt ggtagagcac
      841 ttggtttggg accaaggggt cgcaggttcg aatcctgtct tcccgataat tataaattta
      901 aacacggggc cttagctcag ctgggagagc gcctgctttg cacgcaggag gtcagcggtt
      961 cgatcccgct aggctccatt tcatttaaat aatgaatatc tttttcgcgg tgtagctcag
     1021 ctggctagag cgtccggttc atacccggga ggtcgggggt tcgatcccct ccgccgcgat
     1081 ttttaagagc ctggaccttt agctcagttg gttagagcag acggctcata accgtccggt
     1141 cgtaggttcg agtcctacaa ggtccattgt ggcgttgtaa gtttcatatg gaggattacc
     1201 caagtccggc tgaagggaac ggtcttgaaa accgtcaggc gggtaaaacc gtgcaagggt
     1261 tcgaatccct tatcctcctt tttcatatta tcgcgggatg gagcagtctg gtagctcgtc
     1321 gggctcataa cccgaaggtc gtaggttcaa atcctgctcc cgcaattaaa aactaaaaac
     1381 actggttccg tggtgtaggg gttaacatgc ctgcctgtca cgcaggagat cgcgggttcg
     1441 attcccgtcg gaaccgccat gcgggtgtag tttagtggta aaaccacagc cttccaagct
     1501 gttgtcgcga gttcgattct cgtcacccgc ttttagtttt ttatgggcct atagctcagc
     1561 tggttagagc gcacgcctga taagcgtgag gtcgatggtt cgagtccatt taggcccatt
     1621 agtacaaaat cttattggtt ttggggaagt actcaagtgg ctgaagaggc gcccctgcta
     1681 agggtgtagg tcgggaaacc ggcgcgaggg ttcaaatccc tccttctccg tttcaatatt
     1741 ttgttttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg gcggtaacac
     1801 gggttcgaat cccgtacggg tcattaattt aatatagttt ta
//
LOCUS       DAZULG010000222         1808 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000222 DAZULG010000000
VERSION     DAZULG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.229"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(382..1551)
                     /locus_tag="ACG9CY_002874"
     CDS             complement(382..1551)
                     /locus_tag="ACG9CY_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4939296.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          609 a          283 c          253 g          663 t
ORIGIN      
        1 gaagaaaatc aaggggttta gtttatgatt ttatatcatt tcagtaactc agaatattcc
       61 gacttttttg tacaaaaaaa ttaaccttac ttatatgaag attctaagct cttatttaaa
      121 ttttagacgg gcagttatcc gtagaagttc ttagaatagc taatttacat aattaaatta
      181 tatgaaattt aagtttgaac aatatactaa gaactcccct tactataaag tgaaataaat
      241 tagcatatga ataaccgtgc taccaagctt ttcattaata aaatattttt attaatattt
      301 tataacttta cgcaagatca taattttgtc gggagcgtca ataattttgt gtaaatggaa
      361 aagcccattc tgttgatcaa gttaaaacat tgattctaat gtatcatgta tttctttaaa
      421 acctttatga actcggcata gaaacttttg attatagttg ttgaattgag atactagaaa
      481 tctctccaga gattcttcat taggaaattg ctcttttctt ttggtatatt tctttaattg
      541 tttgttaaaa ccttcaatca aattagttga ataaatagtt cttctgattg acggagggaa
      601 attataaaag gttaatatag caggattcat gagcaattta accactcgtg gatactgctt
      661 tttccattta tctatcataa accttatttg attatttgct tcttcttttg aacttgcttg
      721 ataaactaat ttgaaatcgc tacagatttc ttgtcgatca ctaacacgaa ccttatgagc
      781 gatatttcta gatatatgga cacaacattg ttgaaattga gcatttggat aaacactatg
      841 aatactatcg ttaatgccac ttaaaccatc agttacgact aataaaacct cttttaaacc
      901 acggtctttt aggtcttgaa gaatctcttt ccaaacataa gcagattcag ttggagcaag
      961 actaaaccca agaacctctt tggttccatc taatcgaatg ccaattacaa tataaactgc
     1021 ttcttttgaa acagtttgtc tttttaaagg aatgtgagta gcatccataa aaatgacaga
     1081 gtatctggat tctaaggacc tttctttaaa ggcaataata tcttcagaaa ctattttagt
     1141 catatttgaa atggtttgtg gcgtatagtg atgcccgtac attttttcaa tcaattcaga
     1201 gatttcagac atagtaattc ctttttgaaa tagctgaata atagtggttt ctaaagagtc
     1261 attggttctt ttataagcag gtaaagtttg ttgagaaaac ttaccatttc tatctctagg
     1321 aattaccaaa tttaattctc catactctgt tctgaatgaa cgtgaataat tcccattacg
     1381 ggaattacct gaattgaacc cagcacgatc atatttttca taatcaagaa aagctgttaa
     1441 ttcagcctgc aataatgaat taacagctaa ttctaaatga catcgaaata attcatctaa
     1501 atcaccttta ttgattagtg tttccataat ttctgtagta aaattgggca tgagaaagtc
     1561 ctcctataaa atttcggttg tggtaacttt aattttacag aatggacttt ctttttttct
     1621 accctaaata tctatttaca caaaatattt tacgctatca ttttgtctgt gtagatattt
     1681 cgttatatca ctaaagataa taagatttaa tgacctcatt aatattgttt gctaggtcca
     1741 aactcttatt aaactttctt tgctataaaa cccaaacata gtaataaaag aataacttat
     1801 taccttta
//
LOCUS       DAZULG010000223         1795 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000223 DAZULG010000000
VERSION     DAZULG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.230"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG9CY_002875"
     CDS             131..505
                     /locus_tag="ACG9CY_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4939297.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG9CY_002876"
     CDS             complement(619..1248)
                     /locus_tag="ACG9CY_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4939298.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG9CY_002877"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG9CY_002877"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACG9CY_002878"
     tRNA            1668..1741
                     /locus_tag="ACG9CY_002878"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
BASE COUNT          621 a          264 c          313 g          597 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttct
//
LOCUS       DAZULG010000224         1773 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000224 DAZULG010000000
VERSION     DAZULG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.231"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACG9CY_002879"
     CDS             <1..1604
                     /locus_tag="ACG9CY_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFS4939299.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          641 a          285 c          359 g          488 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct
     1681 ttttacttgt tcacatttta taacggagta tttaagaaag ctattgaata atgattcttc
     1741 catctttttc attcatttac cggttctgtt gca
//
LOCUS       DAZULG010000225         1751 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000225 DAZULG010000000
VERSION     DAZULG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.232"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..576)
                     /locus_tag="ACG9CY_002880"
     CDS             complement(<1..576)
                     /locus_tag="ACG9CY_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4939300.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIEL"
     gene            complement(907..1140)
                     /locus_tag="ACG9CY_002881"
     CDS             complement(907..1140)
                     /locus_tag="ACG9CY_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4939301.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1155..>1751)
                     /locus_tag="ACG9CY_002882"
     CDS             complement(1155..>1751)
                     /locus_tag="ACG9CY_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFS4939302.1"
                     /translation="NKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNIE
                     KQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFSK
                     AYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTNI
                     PIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
BASE COUNT          544 a          310 c          224 g          673 t
ORIGIN      
        1 taattcaatc cacccaggaa cagaaaattg atacaatctt ctggttaata gcatagtctt
       61 ttcttttgaa tagctatgct tcaatatatc atagaatgta gcgtagaatg agtaaatgta
      121 gaagtggatt tcttcttcat taaagactaa attttgattg atcattccga tagtctttaa
      181 cttatctaga caacagactg tctttttcgt cacaaatgat gtaatttttt caacaatatc
      241 ttgatatttg gttccagctg attggaataa aatcaaatcg aatatacgtt tattctcaaa
      301 aatataatta ataaatggat ctaattcatt gaagttctct ctccaacttt tctctatatc
      361 tatattttct aagtttaaga ggtatttggt aaactcttca ttatacatat taataagcgc
      421 attaatatgc ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa aatgtttata
      481 aaaagcaccc gtagttacgc ctgcggtcag gcacagttgc cttatcttag tttgctgaaa
      541 tccttctttt tcaaaatggc ttagtccact ttctattaac ttttggtgag ttataatata
      601 atctctggtt tctatgggta cttcctccta ataagatgaa ttaactttat tatcttacac
      661 aggacttgaa gtttcaatcg ttaacgttta ttactgaact cattccttag agctaaacta
      721 atgagaaaga gcagaataac tgatacaaaa agcaatccta caagaattaa tagactcatc
      781 taaatcattc accttttcta aaataatccg agaaagattt aaaaaaatta tatccagcta
      841 ttaggataag tcctatcata atcactccaa tagccatttt cttatcctcc ctaaaaataa
      901 agtcttttac ttaaaacctt ttttgtaatc gtcaataaat tttttccctt tatcagttaa
      961 ttgaaatcct atatctaaat agtcctcata tctaagttta gtcagcatct cacttacaga
     1021 tgctggacta acacttagaa cttttgctaa atcattattt tttaatttct tttttctttt
     1081 tgataattcc tgaattgcta gtaaataaat tagtatagta gttgttttaa tttgtaccat
     1141 acctaccctc caaattattt cgaaaggcct tcagcaattt tatccaggtt ccatttcatc
     1201 atcgcatagt aactatcacc atcttgtcct ttttctgcaa ttgaatcagt aaaaatcgtt
     1261 gaatagatag gaatattggt atcttttgat actgttttca ttggtctatc gtccacactg
     1321 ctttctacga ataaggaggg aacttttgtt gtgcgtaatt tttcaactaa gtgttttatt
     1381 tgatctggtg ttccttcttc ttcagtgttg atttcccaaa tgtaagcaga aggcacatta
     1441 tacgctttcg agaaatattt aaagcatcct tcacttgtaa caatcatttt tttatcattc
     1501 ggaattgaag caaatttaga tttagcttct ttgtctagag aatccaattt ttcaatatac
     1561 ttatctagat tttctttata gaatttttta ttatcaggat ctttttccgc taactgtttt
     1621 tcaatatttt tagcgtaaat aataccgttt tctaaattta accaagcatg aggatcttcc
     1681 ttccctttct cactctgacc ctctaagtaa ataacatcta tgccatcact tgctgcaaaa
     1741 tagtctttgt t
//
LOCUS       DAZULG010000226         1715 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000226 DAZULG010000000
VERSION     DAZULG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.233"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..808
                     /locus_tag="ACG9CY_002883"
     CDS             <1..808
                     /locus_tag="ACG9CY_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS4939303.1"
                     /translation="LFKNKQNFKEEPGDMRLLPFTSFIIFFLSTFGVSDFAIGTVLYP
                     KLKWVSMKKLPGTLNTQCVVPVAVMALSYITAINVGIKTLAVCIICQIIGAYLGPRFV
                     VKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSNGTASELYGGKLILAGFLLFVYGAL
                     NNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACTFSVPIGSVQFIKFNEYSRKITLFT
                     STFGVLGVLVAVFLVKSLDTYILKWIVILVLLYSAYTMLGSQLKKVTAAN"
     gene            821..>1715
                     /locus_tag="ACG9CY_002884"
     CDS             821..>1715
                     /locus_tag="ACG9CY_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS4939304.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKT"
BASE COUNT          530 a          327 c          380 g          478 t
ORIGIN      
        1 ccttttcaaa aacaaacaaa atttcaaaga agaaccaggg gatatgcgtc tgttgccctt
       61 cacgtcattt atcatcttct ttctttcaac atttggcgtt tccgactttg ctatcggaac
      121 cgtcctttat ccaaagttga aatgggtcag catgaaaaaa ctacccggaa cgttgaatac
      181 ccaatgtgta gtaccggtag ccgtcatggc attgtcttac atcactgcca tcaacgtagg
      241 gatcaagaca ctagctgttt gtattatctg ccaaattatc ggtgcatatt taggaccaag
      301 atttgtcgtg aagttaccag agaaaacgat caaactgttt gtcggcatag gattggtcat
      361 tgctgcattg ttgatttttg caggacaaat gaactggatt ccatcaaacg gaacagccag
      421 tgaattgtat ggcggaaaat taatcttggc aggattttta ctctttgtct atggtgcatt
      481 gaacaatatc ggcattggtt cttatgcgtt aacgatggta actgtctact tgctaggctt
      541 gaatccagtt gcagcctttc ctattatgat gggggcttgt accttctcag taccgatcgg
      601 cagtgttcaa ttcattaaat ttaatgaata cagcagaaaa atcacacttt ttacttcaac
      661 cttcggtgta ttgggggtat tggtcgctgt attcctagtc aaatctttag atacctatat
      721 cctgaaatgg atcgtgattt tggtcttact atacagtgca tatacgatgt tgggcagtca
      781 attaaaaaaa gttacagcag ctaattaagg agcgattcaa atgttatttc ttaaaaaaga
      841 agatattatc aaaagttttt caatgagaga agccatcgat gcggacaaaa aagcactgtc
      901 attgtattca gcaggaaagg caagtgtgcc tcttcgcacc aatatcgatg tgccaaaaag
      961 caacggacaa agcttatata tgcctgctta tgtagaaggt ggcgaaggcg cgctaggtgt
     1021 caaaatcgtt tccgtgtatc ctgaaaacat caagaaaaac ttaccaagtg taccagcaac
     1081 gatgatcgta ttggatccag aaacgggtat ggtatccgct tgtttggatg gtacttattt
     1141 gacacaatta agaacaggcg ctgttcaagg agcagcaaca gaacttcttg caagagaaga
     1201 tgctaaaatt ggcgcgctga ttggaaccgg cggacaagca cagtcacaat tagaagcaat
     1261 gttgaccgtt cgtaaattag aagaagttcg gatttttgat attgattttg agcgtgccag
     1321 ccaatttgct gaaaaaatga tgcaacaatt ttctgtaacg atgcgcccaa caaaaaccag
     1381 tcaagaatgc gttgaaggag cagacatcat tacaagtgtg accacttcaa aacgtgcgac
     1441 attctctgct gagtgggtca aaaaaggtgc gcacatcaat ggagtcggtg cttacacacc
     1501 ggaaatgtgt gaaattccac gagaaataat caaagccgca gatgttgtta tttttgatac
     1561 catggacggt gttctaaaag aagctgggga cttcatttca ccgctgcaag atgggtatat
     1621 tcaaagagac agctatcatg gggaactagg ccaactgatc aatgaagaat tgatcggtcg
     1681 gacaagtgga gaacaaatca cgatcttcaa aacag
//
LOCUS       DAZULG010000227         1712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000227 DAZULG010000000
VERSION     DAZULG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.234"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..521)
                     /locus_tag="ACG9CY_002885"
     CDS             complement(<1..521)
                     /locus_tag="ACG9CY_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4939305.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIM"
     gene            1069..1371
                     /locus_tag="ACG9CY_002886"
     CDS             1069..1371
                     /locus_tag="ACG9CY_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4939306.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            1443..>1712
                     /locus_tag="ACG9CY_002887"
     CDS             1443..>1712
                     /locus_tag="ACG9CY_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4939307.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTI"
BASE COUNT          620 a          270 c          252 g          570 t
ORIGIN      
        1 tccataatta ttcgttcact aactaagtct atccctacaa atggttcatc taaaaatatg
       61 aaatccgcat tttgagcaag acatcttgct aaaagtacac gttgaaattg tcctccagat
      121 aattctccta tttgatgatt agaatatttt cccatatcga ccttttccaa agcctttgaa
      181 actctttgcc attctgcatt tttaattctt tgaaaaactt tcattccggc atatgttccc
      241 atagatacac attcttttac ttttattgga aaagtaaaat caatatcagc tttttgctct
      301 acatatgcaa tcttcaccag ttttttattg atatcttcat tattaatgga gacctctccc
      361 tgatgaggga taatatttaa cattgcttta agcaatgtgg attttccagc accatttgga
      421 ccaataatac ctgttaaagc accttcttgt atttttaaag aaatctcgtt caatgctaaa
      481 aaatcgttat aacaaacatt aagctttttc acttctaaca taactttctc cttctaaatg
      541 ataatgatta tcacgatcat atggtataca aaaattatat tttagtcaac cctaaatttt
      601 atagtttaga tttttttctg ttgctgaaga ttagaatgac aaaactattt gaaactacgc
      661 gtatagtaag tagctttcaa aatatataaa aaaggagcgt tctcaaatga gaacatgctc
      721 ctaaaactac tttattggcg tatggaaaaa ttatacttga agtggatagc gggttgaata
      781 agttgataag catcaaaaca tttataaaaa ttattcaaga ctatgtctgt tccatataca
      841 aatatgaaaa tcagtaaact ccatttaact tttttcgtag tgtgtctttt tatacatgtt
      901 tatcgttatt aatagtgaat aggaaaaaaa ctatttttga tcattgacaa ttaaatacaa
      961 tttttgaata acgatccctg ctattcgagc tagaaactca agtacgccat gacctgatta
     1021 gcgacaaata cttagagatt tctttttgtt ctgggaaaac aaaatgccat gacaatagca
     1081 gcaaataatg atacttccgt aaggctcgcc gtaaactggg gagaggtgaa attcctatgt
     1141 gcttacccaa agcaattcaa aaatttcata actactattt taggagatga ttttatagaa
     1201 tccagagtct acctgaataa aaaacaaacc tgtgcatttc taggaatcga taataatact
     1261 ctagataagt ggattcgtat atataatttc ccagtaataa gagttggtgg tgtagttcgc
     1321 tttgacgtag aagatattaa agactttatg aatacattta aaataacttg aaattttctt
     1381 ctcgccgcat atactaaaat tagtgaaata tactgcctta ctttttttaa ggagtgagga
     1441 ttttgcctac aaagctttca aatgggaaat ataaaactaa tctgagatat ccaaaaaggt
     1501 ttaaagaaat aacaggaatt gcatcagaaa agtatcaaaa aacatttcca aataggcaat
     1561 tggcgattaa agccgaaaat gatatgaaga agaaaataga aaaagttttg agagaggaga
     1621 atgcaaactc attagaatta aaaggaaaaa taacgtttaa aaaattttac gaatctaaat
     1681 ggcttcctag atatgaatta ggtcaaacaa tt
//
LOCUS       DAZULG010000228         1654 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000228 DAZULG010000000
VERSION     DAZULG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.236"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1604
                     /locus_tag="ACG9CY_002888"
     CDS             138..1604
                     /locus_tag="ACG9CY_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS4939308.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          338 g          558 t
ORIGIN      
        1 tttttatgat attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt
       61 aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa
      121 gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg
      181 gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg
      241 cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag
      301 caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg
      361 gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt
      421 tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt
      481 cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg
      541 gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg
      601 gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata
      661 actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca
      721 tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa
      781 ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag
      841 tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca
      901 gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg
      961 taactgctga agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca
     1021 ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta
     1081 cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa
     1141 ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc
     1201 aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc
     1261 tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt
     1321 tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg
     1381 tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag
     1441 ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag
     1501 aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta
     1561 ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa
     1621 caggaattgc ttatttgcag cagttcctgt tttt
//
LOCUS       DAZULG010000229         1642 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000229 DAZULG010000000
VERSION     DAZULG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.237"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..240
                     /locus_tag="ACG9CY_002889"
     CDS             <1..240
                     /locus_tag="ACG9CY_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939309.1"
                     /translation="KKLLKQFAGIGNANVKFICLSCGEKESIPMDVVKFLDDADIAND
                     PNNPPQFTCEKCGKEMYPEYYRNALGIEFKISDVQ"
     gene            394..>1642
                     /locus_tag="ACG9CY_002890"
     CDS             394..>1642
                     /locus_tag="ACG9CY_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4939310.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEK"
BASE COUNT          590 a          250 c          367 g          435 t
ORIGIN      
        1 aaaaaacttt taaagcaatt cgctgggatt gggaacgcca acgtgaaatt tatatgtcta
       61 agttgtggtg aaaaagaatc aatcccaatg gatgtagtaa aatttttaga tgatgcagat
      121 attgcaaatg atccgaacaa tcctccccaa tttacctgtg aaaaatgtgg gaaggagatg
      181 tatccagaat attatagaaa cgcccttgga atagagttta agatcagtga tgttcaataa
      241 gaaggttaat catacaaaaa gtccaccgga gaattcattc tccggtggtt ttcttaaaca
      301 gtaaagaaag tataaaaagt taagggctaa aaaggctgca ctaccagtct tttagccctt
      361 ttcattttgt ttttgaacaa gtaaaatatt actatgaaag cgaatattct cgaagcaatc
      421 ttttttgacg aacaccataa ttgggataac tttgttgaga aatacgggcg aaagattcgg
      481 ctagtcgtca aacgggaagt aaacaagttt agatattgtg gagatattca aaaaggatac
      541 cgactgtttg tctgcgaggg atgtcatcaa acaaagtttg ttcccttgaa atgtcgtgga
      601 aagttctgtc caacgtgtgc aacaggtgaa agccaaaggt ggtcagcggt tgtggcgaat
      661 gatatgttta aagtgacaca tcggcacgta atctttacga tcgatgaggg gttacgggaa
      721 atctttatga tggaaaacta tcgagattcc ttactgaaag gactaatgga tgaagccgca
      781 aaaattattc ttgcctattt tgagaaaaga aaggtgaagg caggtatagt agctacaatc
      841 cacacctttg gatcaaaact agaatttaat ccgcatgttc atatggttgt tactatgggt
      901 ggcttaacaa gagatggaag ttgggaagaa tacgattatt tacctttttc tatgttaaga
      961 aaatactgga aaaatgctgt attgaaacta attagacgaa cgttaggtga gtgggacaag
     1021 caaagagttc aatcaagatt acaagctgcg tacaagaaca atggagaagg gttttacgta
     1081 aatgctccta aaaggagcag gacaaatcta aagtcactac tccaatacat cagtcgttat
     1141 atgaagcgtg gcccgatcgc attaagccga atcttaatgt atgatggcga cactgtcatg
     1201 ttcaactaca tggacaaacg aacgaatact aaagagacga tgaccatgag tatagacgaa
     1261 ttcatagctg cactgattcg ccatattcaa aataagaatt tcaaaacgat tcgtcgctat
     1321 ggaatctata gtcgtagaat caaaacatta atgaaaaagg tcatgaagga gtatcagaaa
     1381 cgagtaaaaa agctgtttat caatctgaat gaggccttaa gaccaaaatc ctgggaagaa
     1441 agaataaccg aagaatttgg tgaaaatccc ttagaatgtt cttgttgcgg tgagtactat
     1501 gaattcgttg ggatgagtgt gcgcaaaaat ggacggttag ttattaaata tgcgaaagat
     1561 aaccatgcaa agcggtttat gcgagaggag aatcgaaaaa ttgaccaaaa agaattcagg
     1621 caacagtatg aagaagaaaa ag
//
LOCUS       DAZULG010000230         1639 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000230 DAZULG010000000
VERSION     DAZULG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.238"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            415..>1639
                     /gene="ltrA"
                     /locus_tag="ACG9CY_002891"
     CDS             415..>1639
                     /gene="ltrA"
                     /locus_tag="ACG9CY_002891"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4939311.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRL"
BASE COUNT          585 a          299 c          388 g          367 t
ORIGIN      
        1 acttggtggt gaaagtccac tacaggcttg gcagtaggaa ctgttagcga aagacaaggc
       61 ggcaatcgtg aggttgtggc tgaaggaagt cggacgcaaa ctcctgaact gacgaacaga
      121 aactagatac aaggctgaat taggtcggat aagtcttcat cacaagacaa agtccaatac
      181 tgcccgaaac ctatacagta aatctagcag ttacatggga ggaaagtttt cactcttacc
      241 gggggagatc tcatgagggt gttgtgggcg ttgaatgaaa acaaaccagc acaacgatca
      301 tggcgacatg atggtgaatc atgagaagtc agccgaggtc atagtagtaa cgacactgac
      361 cgttacgaag gactgaacaa taagaatctt gaataattta ggaggtgtga gaagatgcga
      421 cgaccgcaga aaacaagaaa acttggctat catcaaagag ctagtgtgga acacgaaggg
      481 tatgatgaag tgcgcagcac ttcttgcggt gaacagagga aacaagatgg tgtagaaaat
      541 ctgtttggga gaattctaga tcggaataat ctcaatcaag cgtatcttca agtagtcaga
      601 aataaagggg cggctggtgc agatgggatg accgtcgatc aactgcttcc ttacttgaaa
      661 gagcaccgcg aggaattact atccaattta cgccaaggga actataaacc tcagcctgtt
      721 ttgcgagtag aaataccgaa accaaacggc ggtgtaagaa aactaggaat tcccacggtt
      781 attgatcgta tgcttcaaca ggcaatcaat caagtgttac aaccgctatt tgaaccagaa
      841 ttctcagata atagttatgg gtttcgccca aatcgcagcg cacatgacgc agtgaagaaa
      901 gcaaaatctt attatgaaca aggttaccga tatgtggtgg acattgatat gaaagcctac
      961 tttgacaccg taaatcacga taagctgatg tatttcattg agcggaaagt aaaggacaaa
     1021 caagtcctaa aactgatccg tcaatactta atgagtggtg taatggaaaa tggactcatc
     1081 acaacaactg aagaaggaac gccccaagga ggcaatctct ctcccctgtt aagtaacatc
     1141 tacctgaacg aatttgatca agtgctagaa aatctaggac ataaatttgt tcgatacgcc
     1201 gatgactgca atatctatgt tagaagcaaa cgagcaggac agcgtgtgat gaagaaatcc
     1261 attcaatttc tcgaagaaca actaaagttg acggtaaatc gagaaaagag tgcggtagga
     1321 agtccattga aacgaaagtt tcttggattc tgcattctgc caacaaagaa aggtattaaa
     1381 attcgccctc acgcaaaagc aaaacagcgt gtgaaaagca agctcaaaca actcactaaa
     1441 agaaaccgtg gaagaagtat ccaacttatt ttaaaagaaa tcaagcaact aatgactggt
     1501 tggatcaact actatggaat tggtgagatg aaagaattca tgagggagtt aaacggttgg
     1561 ttgaagcgaa gaatcagaca atatatttgg aaacaatgga agaatccaag aaccaaaaga
     1621 aaaaatctga ttcgtttag
//
LOCUS       DAZULG010000231         1638 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000231 DAZULG010000000
VERSION     DAZULG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.239"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..400
                     /locus_tag="ACG9CY_002892"
     CDS             2..400
                     /locus_tag="ACG9CY_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4939312.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="ACG9CY_002893"
     CDS             384..1079
                     /locus_tag="ACG9CY_002893"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS4939313.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          619 a          238 c          323 g          458 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1501 acttttttaa attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat
     1561 aaaaaaaggc cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt
     1621 tccaaaaatc acttctca
//
LOCUS       DAZULG010000232         1634 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000232 DAZULG010000000
VERSION     DAZULG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.240"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1398
                     /locus_tag="ACG9CY_002894"
     CDS             <1..1398
                     /locus_tag="ACG9CY_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4939314.1"
                     /translation="IFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKW
                     PEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDASEGIKNKEDL
                     PKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG
                     EKVEVGGTVDLTDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKD
                     TVKVPVEVTDNRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVD
                     VSTAGNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNA
                     EVTVTLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNE
                     KDSEKAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLG
                     TLGLLGYKNRKKENE"
BASE COUNT          649 a          239 c          353 g          393 t
ORIGIN      
        1 atcttcaccg caggggaagg aacaagtatt gaaagtggaa caacggtctt tgcagtgaaa
       61 gacggcgtaa gcttaccaga agataaactt ccagtgttga aagcaaaaga tggttataca
      121 gatgcgaaat ggccagaaga agcaactcaa ccaattacag cagatgatac agaatttgta
      181 tcaagtgcaa caaaactgga tgataaatct gacgctgaca aatataatcc tgaaggtcaa
      241 aaagtgacta cagaattgaa taaggaacca gacgcatctg agggaattaa gaacaaagaa
      301 gatttaccaa aagatactaa gtatacttgg aaagaaaaag tagatgttag cgcagctgga
      361 aataaaaaag ggactgttgt agtgacatat ccagatggat catctgatga agttgaagta
      421 gatgttacag tgacagacaa tcgttctgac gccgataaat atgagccaac agtagaaggc
      481 gaaaaagtag aagttggagg cacagtagat ctaacagata atgttactaa cttgccaaca
      541 ttaccagaag gaacaacagt aacagatgtc actcctgatg gtacgattga taccaacaca
      601 ccaggtaatt acgaaggggt tatcgaagta acgtatccag atggtacgaa agatacagtg
      661 aaagttccag tagaagtgac agacaatcgt tccgatgccg ataaatacaa tcctgaagga
      721 caaaaagtga ctacagactt aaataaagag ccagacgcat ctgagggaat taagaacaaa
      781 gaagatctac caaaaggaac tacttatact tggaaagaga aagtagatgt tagtacagct
      841 ggaaacaaaa aagggattgt agtagtaaca tatccagatg gatctaaaga agaagtagag
      901 gttactattt ctgtagaaga taaaaaagca ccgaataaac ctcaagttga tcctattaca
      961 gagggtgacc aaattgttac tggtaaaact gaaccaaatg cagaggtgac agttacatta
     1021 ccaaacggga gtcaatacca tgggacagct gataaaaatg gtaattttac agttaaagtt
     1081 cctaaattag aggcaggaac aaaagtgata gtaactgcaa ctgatgaatc tggtaatact
     1141 agtgaaccta ctaatgtagt tgtttcttca aacgaaaaag acagtgaaaa agccgttagt
     1201 aaagataata agactgacaa tcaaggtagc aagcaaaaca aaaatagagg caaaagcagt
     1261 cctcaaaagc agtcctcaaa agcttatcct aagacgggag agattgatag taatatcttt
     1321 actattagtg gtggtttaat cctgttagga actttagggt tattagggta taaaaatcga
     1381 aaaaaagaga atgaatagaa aatcaatttc ttaaatcttc tctgaaaaga ggaagaatct
     1441 ttttcttcct ctttttgttt tttattttgg gagataggtt gttagttaaa aatgaagata
     1501 cttttcatag aagggaaaag aatgtgatta agtgaaaaaa atagtaatta ttggatcaaa
     1561 ccattcgggg ctagctgcag ctcgatttct aaacttatgt gattcaaatt atgaagttat
     1621 tctgatagat aaaa
//
LOCUS       DAZULG010000233         1585 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000233 DAZULG010000000
VERSION     DAZULG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.242"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..242
                     /locus_tag="ACG9CY_002895"
     CDS             <1..242
                     /locus_tag="ACG9CY_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939315.1"
                     /translation="LGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHDVDRNEIL
                     EKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(604..843)
                     /locus_tag="ACG9CY_002896"
     CDS             complement(604..843)
                     /locus_tag="ACG9CY_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939316.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1129..1443
                     /locus_tag="ACG9CY_002897"
     CDS             1129..1443
                     /locus_tag="ACG9CY_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939317.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          540 a          216 c          279 g          550 t
ORIGIN      
        1 ttttaggaaa aaaatatatt tgccaaccta ttggcttgaa acaaaaagtg attggagaag
       61 taatcagtaa attagaaaat tgtgttgtga tctgcgtaga cgaatatcat gacgtcgatc
      121 gcaatgaaat attagaaaaa tgcggaaaag tagtggcaaa atatactcac gtttatgagt
      181 tggaaacaac agaatatcta tataactata gcaacaaata tgaacatact ttagtttcat
      241 aaagatttaa ataaagtgcc ttcaaaagcg agaagaccaa gcttttgaag gcactttatt
      301 tagttggcag tgattgttca tgatttttga tttattcaaa aggttcgcct agagaatctg
      361 tctctgtaac gaggcctggt ggagaaactc gataaaaacc tatactgcca gaactgtcct
      421 attttcggat agacaaacta gtagttttaa aaagatcatc cgatccaatc atttcaaaaa
      481 atgcaaaact gatatcagga tcatttagct caggcttgtt atcgtaaagc atttgtaaag
      541 gttgcattta gttaggagta aacgcgaaac tatcatcatt atttgctgaa gaagatgaag
      601 aagctaattc agagtttgtt tgaaataaag gagacgtgct gacagtttct ttaccctgtt
      661 ttttgcagag gtagaaactt ttatatcgga agtttctacc ggagaatttt tctcatttgt
      721 agaaattgtt gaatcgatac tattcgaaat ttgcgtatct gaaggtatgt tctttgatgc
      781 agaagtccca caagcagtaa gtaacaataa atttgataaa ctgataaaca gaattctttt
      841 cattatgtac acactcctta tagtatcgtt tagcatacac ctatgctcag tattaggcaa
      901 aatcaatgca aaactatcaa gtcttttata gtatctatta gtcttagtaa tacatatatg
      961 gagagggtga tatcccagtt caaaccagat acattggtta ccatctgtat taacgattat
     1021 gcctctaaac gcttttttta tgaaaaaatt tataagtgaa aacgtaggta tgatattatc
     1081 tagaggtaat ttttttgggt atttaaatag ttgctaggag gatagctttt ggatttttac
     1141 agtaatttta tcttaataat agcaatatta ttacttctaa atatttggtt ttttgataag
     1201 agtagaaatg cagggatagg ttttagaacc aaacgaagta cgtcttctga aaaaaagtgg
     1261 gtatattcac agactatttt ttacggaggg gttatttcaa tcagtttact ttcttcaaca
     1321 ctttattctt ttaatgttat agacgtttcc atgtcaaatt ttatatctat tattggaatc
     1381 cttatttctg caattattac acaactactt cttgtgtttg aagagaagtc aaaaaataat
     1441 taaagcaatt atgcctttta gtacaggaat tattacttct tacaaatgta tagcgaatgt
     1501 gaagcctgct gttttaattg ttctagaaaa acttttgagt gaacaattat tttatgttag
     1561 gatgaattaa tttctctaga gttta
//
LOCUS       DAZULG010000234         1579 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000234 DAZULG010000000
VERSION     DAZULG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.243"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACG9CY_002898"
     CDS             complement(138..428)
                     /locus_tag="ACG9CY_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939318.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACG9CY_002899"
     CDS             complement(546..806)
                     /locus_tag="ACG9CY_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939319.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACG9CY_002900"
     CDS             complement(943..1356)
                     /locus_tag="ACG9CY_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS4939320.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          594 a          251 c          231 g          503 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaa
//
LOCUS       DAZULG010000235         1558 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000235 DAZULG010000000
VERSION     DAZULG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.244"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..841)
                     /locus_tag="ACG9CY_002901"
     CDS             complement(<1..841)
                     /locus_tag="ACG9CY_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS4939321.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRI"
BASE COUNT          500 a          312 c          266 g          480 t
ORIGIN      
        1 ctattcttga gctggctgca gctagtgagt gtctttcatt tatccataag ataaagcttg
       61 gattcaataa ataaataaat gttttggtta aagctgtgac cagcaaatga ggaaccgtgc
      121 gagccagttg atctgccaat gcaaacattt ctttaggtga aggcgctggc gtgtctgcca
      181 acggatactc cagtcccaat gcttggtaac cgtgcatacc atagcagtgg tacggtagta
      241 attctaacgg tacgaaccga tcaagatctg ccacaaaaca agcaatcgtc agcagctcgg
      301 tcaggttcat attgatacct ggaaccacag gaactcgaat tcgcacttcg cagttaggat
      361 actcaattaa aaagcgacga atattcttca aaatttgttg gttatctaag ccggtccatt
      421 gtttgtggag cttgctggta aacattttta catccacaaa caatgtatcc acatagggag
      481 ctaccttttg gatttcatta taagcaccac aaaagctggt ttccagtacg gtattgattc
      541 caatgtattt cgatttttgc aaaatttctt tagcaaaatc cgcctgaacc agtgcctcgc
      601 ctccgctaat ggtcactccg ccatccgagt gaaagtaaaa aacagcatcc ttctcaatct
      661 catccattac ctcttcagcg gtcatggtct ggccaaatcc tttttcaatc tgcttgcttt
      721 gtgattcggg aatcgaacac caggcacagc gtaatgggca gccctttaaa taaacaacgg
      781 tccgtaaacc agcaccatca tgtagagaac cctgttctat tcttaatact gttccttcca
      841 tatagatttt tgaattacac actagtttaa actaaaacac acaaggaaaa acttaccatt
      901 taatcataaa attagctcat ataaatatac taggatgtaa aatagactgg tcaaaatctg
      961 gtcaaaaaag aggactaata caggtagtcc tcttttttgt tattttaata ttctaaatca
     1021 ttttttattg gtttattccc ttattcgatg tagactcaaa tcactatatc actagatatc
     1081 aaacggcgtc atacggaatc aaaaacatca aatttgacct gttaaatggt cattgaataa
     1141 cattcaaaat caataaaacg gagtcagaca cggagtcaaa aacgctgaga gtaaaaaata
     1201 tatacagcct tgtaggtaaa gtaccacagg gctgtatatt ttttatttca gattacctta
     1261 aatgattcca tttacttttt atctcatttt ttcctactag ttagtatcaa atactctcct
     1321 attttctcaa taaactattt ttggatacat ggtatctgaa acagctaaaa ccttgtcatt
     1381 acagcaaaaa atcacgttat ccagactgta gacaaagtcc attttatgga tgatgtctac
     1441 agtcttttct tttataatag atttatgaaa acgaatgcaa ggagtgatag ctatgatgtc
     1501 taaaaacaat acgaatggta gaaatcaatt tgcgatgctt acgattgatg atctagtt
//
LOCUS       DAZULG010000236         1543 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000236 DAZULG010000000
VERSION     DAZULG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.245"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1163
                     /locus_tag="ACG9CY_002902"
     CDS             <1..1163
                     /locus_tag="ACG9CY_002902"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4939322.1"
                     /translation="GKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQIVA
                     HAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQ
                     PNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYM
                     RSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYE
                     KLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTG
                     NISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNID
                     SFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          554 a          271 c          250 g          468 t
ORIGIN      
        1 aaggaaaaca tgctaaaaaa agaaaatatt catttttaat gatcgttgtc gtgttgttta
       61 gccttagtgc gttctctaca ggaactattc ttggatggca attttacaat aatgaacact
      121 ctcagatagt tgcccatgct caatcagctc aaaaaaagtt gatactgact accaatgaac
      181 aacagctcgt tgaaaaaatc gatcaactat taagaaaaaa tgattatatt ggctcaattt
      241 acgtaaagaa aaataatcgc atcatcatgg aaaaaggcta tggctatgcg aataaactca
      301 cgcaacaacc aaatgatcct tctctttatt atcagattgg ttcggttcaa aaagcattga
      361 ctactctttt gattttaaaa caagtcgagc gtgggcacct ttctttagat actaagctgg
      421 ctcaattcta tccacaaatt gctggcagcc agcaaattac tatccgtgac ttgctgtata
      481 tgcgctcagg cttgcaccga actgcttctc caaccatacc gatgactgat gaagaagtcg
      541 ttcaatttgc gattcagcac ttaaaattta cagattatca gacctctcat tatgattcat
      601 tgaactttac catattgact ggaattttga tcaaattaac aaatcaaccg tatgaaaaat
      661 tattgaccga tgatatcatc gaacctttag gattacagca tacaaaattt tatgaccaag
      721 tcaaaaattc atccaatcat gcactatcct atcaaatgtc taaagaggat gattacttta
      781 aaccactgac agaatctgaa acagagatcc ataacgaatt agggactggt aatatcagta
      841 tgtccgtcta tgatttaaat acgctgttta cacaagtttt atctggaaaa ctagtttcca
      901 aagacttact cttctcatta tggcgtgaaa atgcgaatgg acatccatat agtggtgggg
      961 tatatagcgg agataattat atcctgtcgc aaggaaatat cgactctttt gatacatctg
     1021 ctatatttag caaagattca caggatgcta tcatcatgga aagcaatata aaagctgaca
     1081 aaactatcaa actgcctact gctgatctaa gaaaccaaat ctatgacctg atggaaggaa
     1141 ctactaagtt ggcaaatagc tgatatgatg aaaagatagc aaatactact tctttgaata
     1201 gcatttgctt aaaaaaagta ctcgactaaa cgtaggaaag agtgtgggac aaatctaaaa
     1261 atcaattttt gctacagact ttaattccaa ataaacagaa cagaaagagg tgttcttctt
     1321 tctgttctgt ccaaattatt ctaagatttc tctttaccaa cattatttga caaagggcct
     1381 attaaatcta aaatagtatc tttagattgc taaaatatgt gctagcaaat cattcatggc
     1441 ttgttcttat ttttaatttt atgcaataaa aaagaagaac aattagcgtg attatagaaa
     1501 taaataatga gattttagaa aaaaatgtgt atgaatacct gat
//
LOCUS       DAZULG010000237         1530 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000237 DAZULG010000000
VERSION     DAZULG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.246"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1445
                     /locus_tag="ACG9CY_002903"
     CDS             <1..1445
                     /locus_tag="ACG9CY_002903"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4939323.1"
                     /translation="NLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFF
                     PFISVMLLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETI
                     ELQQEINSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILV
                     GVHLLLTGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQ
                     EGSDSPLKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLV
                     SRLYDYDEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDE
                     EVKKAARLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDE
                     ITASLDAENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNE
                     LYRRSEIYKNLIKKTKLSEKFVYEKEAQSR"
BASE COUNT          524 a          226 c          287 g          493 t
ORIGIN      
        1 caaatttacc attatcctat ttttcaaaac ataatttatc agatttatct caaactatca
       61 tgtctgacgt tgaaggtatt gagcatgcga tgagtcatgc aatacctaaa gccggcggta
      121 tggctctgtt tttccctttt atttcagtga tgcttttggt tggtaatgtc aaaatgggat
      181 tagctgttat tttgccaacg ttatttagtt ttgtcttaat cttgttatca aagaaatccc
      241 aaacgaaagc caatactaaa tattacgata ctttgagaga aaactcggaa gaatttcaag
      301 aaactataga attgcagcaa gagattaata gctttaatct atctaaaaaa gttcaagaca
      361 gacttttcaa aaaaatggaa gagagtgaaa ggattcattt aaaggtagaa ttaagtactt
      421 tttcagtcat ggccttatcc tctattttct catatgttag tttagcagta gtcattctag
      481 taggtgttca cttactgtta acgggtgaag tgactatact ctacgtcgtt ggttacttac
      541 tagccgcaat aaaaataaag gattccttcg attctatgaa agaagcagtg cttgaaatat
      601 tttatttagc tcctaaaata cagcgaatta gagctatgaa ggaaaccagt atccaagagg
      661 gatcagatag tcctctgaaa tcttttgaca ttgagttgcg agatgtctct ttctcatatg
      721 ataacaatac cccaatttta gatcatatct cttttacggc taaacaagga gaagtgactg
      781 cgttagtagg tgctagtggt tccgggaaaa ctagtatctt gaaattagtg tctagactgt
      841 atgattatga tgaaggctgt attttaattg atgggtatga tattaaacgt gtatctccag
      901 cttctttgtt tagcaaaatc gctattgttt ttcaagaggt aactttgttt aatacaagta
      961 ttttggaaaa tatacgtatt ggaaatagtc aagcgtcaga tgaggaagtg aaaaaagcag
     1021 ctcgtttagc taactgcgaa gactttattg aaaaactacc tgacggttat catacattgg
     1081 tcggagaaaa cggaagttca ctttctggag gagagcgaca acgcctatcc atagcacggg
     1141 cttttttgaa aaatgcgcca attttaattt tagatgaaat tactgccagt ttagatgcgg
     1201 aaaatgaaaa acgtattcag gaaagtttaa atcgtttaat acaagataag acagtactaa
     1261 ttatatcgca tcgcttgaag tctattgaaa aggtaaataa aattgtagtg atggatcaag
     1321 gaaaactagt tgatcaaggt actcataatg agttgtatcg aaggtcagaa atatacaaaa
     1381 atctcataaa gaaaactaaa ctatctgaga aattcgtcta tgaaaaagaa gctcaatcac
     1441 gatgatgaaa taggctttaa attaacctac aatttttggg atatagttat aattcttttt
     1501 caaaaagaat aattttatta aacagcatct
//
LOCUS       DAZULG010000238         1526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000238 DAZULG010000000
VERSION     DAZULG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.247"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACG9CY_002904"
     CDS             191..445
                     /locus_tag="ACG9CY_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4939324.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACG9CY_002905"
     CDS             446..763
                     /locus_tag="ACG9CY_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4939325.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..>1526
                     /locus_tag="ACG9CY_002906"
     CDS             1322..>1526
                     /locus_tag="ACG9CY_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939326.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFR"
BASE COUNT          539 a          219 c          292 g          476 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaa
//
LOCUS       DAZULG010000239         1518 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000239 DAZULG010000000
VERSION     DAZULG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.248"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG9CY_002907"
     CDS             <1..1087
                     /locus_tag="ACG9CY_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4939327.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1294..>1518)
                     /locus_tag="ACG9CY_002908"
     CDS             complement(1294..>1518)
                     /locus_tag="ACG9CY_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4939328.1"
                     /translation="SRIKKLAEIAGVKPIHMHGLRHSHVAFLIEHNKNIYAISKRLGH
                     ASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          530 a          267 c          283 g          438 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa tccatgtaaa catggtttat
     1141 tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc gtccattttc
     1201 gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa aaataggcta
     1261 tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg taatttcatt
     1321 tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag tagttttaat
     1381 agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt caattaaaaa
     1441 ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg caatttcagc
     1501 tagttttttt attctgct
//
LOCUS       DAZULG010000240         1482 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000240 DAZULG010000000
VERSION     DAZULG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.249"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..1460
                     /locus_tag="ACG9CY_002909"
     CDS             join(121..562,562..1460)
                     /locus_tag="ACG9CY_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS4939329.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          609 a          198 c          259 g          416 t
ORIGIN      
        1 ttcccaatga aactcgagac acttatttta aagaggaaac aatgtggtat ttaaatagcc
       61 ctcttgttga tgtgcacccc aaaagttaga ctagaaatct aattaaaagg ggtgcttttt
      121 ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt aaataacgaa
      181 ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt tagacattgg
      241 gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa aagctacact
      301 acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc tatccaagaa
      361 actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat agagcaatat
      421 gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat tagtatgaat
      481 tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga agttattcgt
      541 ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga acaagatttg
      601 agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt aaattgaatc
      661 tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta aaaaaattag
      721 ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta aaagaatcaa
      781 gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga tatacaatca
      841 atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa atccgaataa
      901 agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg ttaaaacgaa
      961 atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa ttcaagataa
     1021 aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa ataattagtt
     1081 atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa caagcaatta
     1141 aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg cagtatcaga
     1201 tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg tctagaaaag
     1261 gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa tcagagtttt
     1321 tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat gagtatatta
     1381 ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca gtagaatatc
     1441 ggaaacagtc cattaaatag ttcaactttt ggggttcagt tc
//
LOCUS       DAZULG010000241         1474 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000241 DAZULG010000000
VERSION     DAZULG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.250"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..499
                     /locus_tag="ACG9CY_002910"
     CDS             89..499
                     /locus_tag="ACG9CY_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4939330.1"
                     /translation="MIKVYTSPSCTSCRKARAWLKANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            513..869
                     /locus_tag="ACG9CY_002911"
     CDS             513..869
                     /locus_tag="ACG9CY_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4939331.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          542 a          209 c          272 g          451 t
ORIGIN      
        1 tgattttttt tatattttat tagctaataa ttggtatttt aaaagaaatt actgtagaat
       61 tggattataa ttaaaaagga gtgagaaaat gataaaagta tatacttcgc ctagctgtac
      121 ttcctgtaga aaagcaagag catggttgaa agcgaatcaa cttgaatttg aagagaagaa
      181 tatttttgcc gaaccgttaa cagaaagtga gatcaaaaag atcttggcag cgactgaagg
      241 aggagttcaa gatattattt ccactcggtc gaaaatttac gaaaagctgg gcatagattt
      301 taacgaatta actttaaagc aaatgatcgt cttattcaaa gaatatcctt cattattgag
      361 acgacctatt ttgattgatg aaaaaagaat actaatcgga tttaatgaag atgagatccg
      421 gtcatttgtc cctcgtgaaa ttcgatgtgt tgccagaaat ctgatctatt tacaaaacaa
      481 taacatctta aattcatagg gagggagcct tcgtggaagt gaaagtctat ggatcagcgc
      541 actgtgtaac aagtctgaag gcgaaacaat ggttagaaca ccatcaattg ccttatcaat
      601 ttgttgactt agaagaacgc gatttaagta cagaagaagc agaagaatta tgcagttttg
      661 aagaagtgca agcagaacag ctatttgcga cttggtctga ggcttttcac aacattgcga
      721 ttgacctttc ctgtgtagga aaaaaacaac tgattttttt atgcacacaa cataccaatc
      781 tcctcagacg tcctttgatt attatcgatg aagttctatt tattggatac aatgagcagt
      841 tgatggaaca gcttatctta aataaataga ctttaactgt tttaatcaag tcactgagca
      901 tttcatacag ttgtaacagt cgaacactta gcatcaagcg tgttatgtgt tctttttttg
      961 actttatttt tctactatag agacatgctc aatttatgtg gtcaaaattc aaatctaggt
     1021 ggggatatag agttaaaaat atattgacat gtgtaaattt acatgatata ttcatttctg
     1081 ttgttttaag tagcaaagca ataaaatagt attttaaaaa acatataaaa tagttgttga
     1141 catgttagaa atgacatgat aagataactg agttgttaaa aaagaaccac ttgtgaagct
     1201 tgattctatc gaaaaaaact tcaaaaaagt tcttgacaaa taacaaatga taagttatga
     1261 taaatgagtt gctgtaagat ttcgaaaaac gaaatcaaaa aaacttttaa aaaagagttg
     1321 acaattactt gtctctctga tatgatataa aagttgctac ggcagccaaa aacgaatcgg
     1381 ggaacactcc tgatgaagta gacctttgaa aactgaacaa agtaagacaa accaaatgtg
     1441 tagggcgtct tgattcaatt caagacaaca aaca
//
LOCUS       DAZULG010000242         1459 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000242 DAZULG010000000
VERSION     DAZULG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.251"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..>1459
                     /locus_tag="ACG9CY_002912"
     CDS             148..>1459
                     /locus_tag="ACG9CY_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4939332.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIE"
BASE COUNT          534 a          199 c          268 g          458 t
ORIGIN      
        1 ttgcaatatt ctcaaaaaaa agataaaaaa tgagaataac tgatgaaaaa acaagcataa
       61 tccatcataa aatcagtata ggtgaaaaga tgaactttca tctgcatatc aagtaattgg
      121 tatgaggcat tttttgaggt gtaggatatg aagttggatt accttataga agaggaagat
      181 agagaaaaac tgctgctagc tagatattta gaatttgaga aaaagaatta tgtagtgctg
      241 cctaagttat atgaaacatt aggtttgtct aaatttaaaa caagaaatta tatcatagaa
      301 ttaaataaag aatttactga attcaaaaca aagccagaga tccaaatttc agaagaagca
      361 gagatccatg tcgaacgtct gaatttgacg gatatcaaaa atttaagaag aatctacttt
      421 caacgatcaa aaattgctcg actgttaacg gagatcgtgg attcaaaagt aacgatcgaa
      481 aaatttgcag agaatcattt tttaagcata tcaagagcct atgtaaaaag aagagaatta
      541 gtctcttttc ttgaacagca tcaaataaag ttaaagaaaa acatattagt tggggaagaa
      601 ataaacatac ggaacgtatt atttggggta tatttcgggg tctttaatgg tttttctatg
      661 ccatttagag aaagcgtcat gaatgaagca aagagtatcg tgcaatattt tgaatatctg
      721 ttcaatttaa aactgtctgc aacaaaacga atcaaattca atttgctaac cactattagt
      781 ttgttgaggg cctatgggaa tcaaccaatc ggaaaacgtt tcttttcaga tgaatacttt
      841 aagagctcag aaggaatgaa gatcatcaag gatttatcac atatttcttt tcttcatatc
      901 cctaaagagc agatcattga tgagatttct tatatcatgt tgtttttgat cacggaagaa
      961 gaatttgaat tagatggcgc atttggactt gcggaagctc attttcatga tatccacatg
     1021 attagtcaaa aaatgtcagt ggaattgatt gatcaattac cattttcgat tgaaatctct
     1081 gaagaagaaa gactatatat acgaaaagaa cttcgtatgg gtatgatgca tgtcaatcga
     1141 aaaaattgga tattcgactt tgagatgact agctttacgt ctaaaaagca gctacagttt
     1201 ttcttcgaga cgtatccaat attcagtaca gcaatccaaa aggtcataca agagaataaa
     1261 cgctatttct tgggaaaaga tgaacgtttc atgattcgct tattttatga ttatctcttt
     1321 ttattggtcg gtatttgtcc ggtatcatat tttgaagcac cgattcatgt ctgtattgat
     1381 ttttcgcaag gatctgctta taccgattac attatttctc aagtgaaagg atttaaaaat
     1441 tacaatattg tgattgaga
//
LOCUS       DAZULG010000243         1450 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000243 DAZULG010000000
VERSION     DAZULG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.252"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..970)
                     /locus_tag="ACG9CY_002913"
     CDS             complement(<1..970)
                     /locus_tag="ACG9CY_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4939333.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYV"
     gene            1153..>1450
                     /locus_tag="ACG9CY_002914"
     CDS             1153..>1450
                     /locus_tag="ACG9CY_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4939334.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQ"
BASE COUNT          433 a          278 c          213 g          526 t
ORIGIN      
        1 ggacataaaa acttgatcct cctgatactg caccttttcc tggattttct tttgaaaaag
       61 gaatcagtgg agccatgcgc catttgtcag atgcatcagg aaaattcaaa gacatattcg
      121 tagcatacca gccgccagca acaaacattg cagattcttg aaatgctttc tcagcatctt
      181 gtgcaccgta gaaatgtaca atatctgagt cgatcatttc tttgattaac tttgcagcat
      241 gtttcgcttc tttagtattc agacggatat ttccttcttc atcaagcaaa gattgattct
      301 gctgggctag catgttgata aacaattctt ggtccccaga ttctggcaag gcaattccaa
      361 aactaccagt tttttctttt aatgcttttg ccacctggat atattcttcc caagaactga
      421 tttcttcaaa atctacacct atttcagcaa atttttttgg ttgataataa caaagtgctg
      481 gggtaaaatc actagcccaa ggaataatcg tttcattact cgtttgttga cgcaaaatat
      541 tcaatttttg atcataaaac tgatcgccat atttgtcata aaatccatat tcatcagcac
      601 tataaaaagc atcttgaaat ttttccagca cacctgctgc tgaagtgtct gccatggaga
      661 taatatccgg catatcttcg tgtgttacca attttggggt gatcatttgt actgttgctg
      721 ttcccgaagc tacattcaca atttttaagt caatatttgc tccagttttg cggttataga
      781 tttttgcagc attttcaaca tagtttgtca gtttgccgtt tgcgacatat acttctaatg
      841 tagtggcttt ttttatcatt ggatctgcga tgtttgctgt tacttcttcg ttagtttttc
      901 cacatgcact taagctgaat aatagggtaa tgtttagcaa agctatcacc aatttgtatc
      961 tttttttcat gtctatccct tctttctttt caagttctta tataaaacgc tttcacttat
     1021 gtatattaac ggaattagta acacaaatat ttatataaaa aaatatttta cttgtcgttt
     1081 tggcttttca tttttgttat aatagtcatt aattataaat agaaagcttt ttatataaaa
     1141 agagaaagga aagtggttct tttgttaaca aacaaccaat cctccaatct tgtcaaaaaa
     1201 acactaacct attccataga tacccatctt catttatctg ctgctcccta ttttcgacta
     1261 ctaataaatg aaagcgctaa tttatatgct gaatttgcac ataaatctat agaactagat
     1321 aatccttccc ttttattcat tgataaacag caagaagtcc aaatcttttc tactcaaaag
     1381 gaaacacaaa cttttatcat tatccagttt tattttgatg agcaattatt agcacatatt
     1441 acgggacaat
//
LOCUS       DAZULG010000244         1424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000244 DAZULG010000000
VERSION     DAZULG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.253"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            313..855
                     /locus_tag="ACG9CY_002915"
     CDS             313..855
                     /locus_tag="ACG9CY_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939335.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1011..>1424
                     /locus_tag="ACG9CY_002916"
     CDS             1011..>1424
                     /locus_tag="ACG9CY_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS4939336.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMA"
BASE COUNT          543 a          173 c          278 g          430 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcaa gttcttcgtg ttctcttaga
      181 cataaaaatt cctcctggta tgttttcttc tagtctacac accttaattt ttctgtctag
      241 ttaattgtag cctatccagt ttggaaaaat aaaaaattat atttcatcaa cgtttcataa
      301 aaggtgtgta gaatgttatt gaaagtagct ataaaatgct attgtgtaaa aaataaagga
      361 ggaataattt atgaaaaaat agtaccaaga ggagcatgcc tattacttgc attatgtttg
      421 agcttaagcg cagttgggtg tcaaaaaagt aatgaaaaca ataaaataaa agaaggacaa
      481 tccataagtt ctgaagagga aggcatgtca attaatgcaa atggcaaaaa tgaaactttt
      541 aagccatcag attatacatt ggaggcaaag aaagaatatg tttatgaata tttagggctt
      601 aaatttaaac tctcagacaa atttagaaat tatatagctg ataaaaagat agcgatgtta
      661 gatgatcaaa gtcctataga taaagaacta aagtatgcta ttttgacatt tgagaaaatg
      721 acagaagaac aaaagaatgc agttatagaa aagatgggcg atggatataa aaattggcaa
      781 aatgagcttg aaagaattgg gactattggt atatttgaaa aaaatacatc agaagaaaaa
      841 aatctaaaat tataaaatgt gatactcata ctaaaatagg agtttcaagt gatggaaaat
      901 acgattgcta tttaagcaca aatagcggag ctgaaagtaa tcttctggat gaattaaaaa
      961 gaactgagat tcaaattata gataaaaaag aacgtcctaa aaatggtttt gtgctatcag
     1021 aaaaaactga tttagaaaat acagaagcat ttaacaaaga atcggttaaa gatttacgta
     1081 aactaccaac aaaggacata aatggcaagg attttacaag taaagatttt gagaagtatg
     1141 atcttactat ggtaaatgta tttgcaactt ggtgtacagc ttgtgtaaag gaaattcctg
     1201 atttggttga ggttcagaat gaaatgaaaa gcaagggtgt aaatatcgtt ggagttgtta
     1261 cagatcctgt tgatgataac ggtgaaaaca aggaagcaat tgaaaagtca aagttaatac
     1321 atgaaaagac aaaggcttca tatcctttct taatgccaga tggaatggca acactttttt
     1381 tgacaaaaat tataaggtgt tgtgaatgct gcggtgttat ggct
//
LOCUS       DAZULG010000245         1414 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000245 DAZULG010000000
VERSION     DAZULG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.254"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1283)
                     /locus_tag="ACG9CY_002917"
     CDS             complement(<1..1283)
                     /locus_tag="ACG9CY_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4939337.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          390 a          277 c          306 g          441 t
ORIGIN      
        1 ttcttagttt tcttgaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga
       61 aagtttctaa agccataggc aattcgtttt agtactttga tatggttgtt caaacattct
      121 atagggccgt tggaataggg aactcgcaac gctcgtttga tttctgttcg atatttcttg
      181 aaggtcgtga acactggttg atagtgctct ggcaactcct tgtagtcttc ttttaggagc
      241 gcgacgaatt caggaacatc ttgtcttctg atagcggaaa gaaaaacctg gtagacttcg
      301 tagcctcgct ttaaggagtc atcataatat aatagccgat cgactatctc cgcttctgtc
      361 agatgatctc gaaatgacgg acgccagtag cgatgctgac cattcaattc ccacgcattc
      421 ttttgaagta gtctccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag
      481 gtcttcatta cttgaattcg gtggttggta aaggcccgac cgatgtgttg gctaatatga
      541 aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt
      601 gtatagaaat ctgaaaccac tagacgtact tgttcacgga cttttctagg gtagcgcaag
      661 aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc
      721 ttgccgtcca tcatgacaaa gctcatggct cccgagacgt ttttcaccga tttgaattca
      781 tcgaaacaca ccacttctgg taacggctgg ttcaaagaaa cggttttagg ccgaaggctg
      841 cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcaat caaagacata
      901 gaaataggct cacttaatag ctcaagaatg gcttgtttga ctctgcgggc aatggagcag
      961 cgacgatcgc taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag
     1021 cgctgtttgt taatccgcac aatggtcttg tagttactga catcattcaa taagatggat
     1081 acttttttcc agccccattt aatgatcgtt tgcttatttt gatagtggca atggaagcat
     1141 tcctctggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attaatgcgc
     1201 tctttgcgaa aggcatcttc atgaaaggta agatttaggt ctagaatatc tagtaactct
     1261 ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta
     1321 ttctaaagga tttcaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa
     1381 cagaaggcgt caccaacact agatattata gatc
//
LOCUS       DAZULG010000246         1390 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000246 DAZULG010000000
VERSION     DAZULG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.255"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..459)
                     /locus_tag="ACG9CY_002918"
     CDS             complement(25..459)
                     /locus_tag="ACG9CY_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4939338.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(559..>1390)
                     /locus_tag="ACG9CY_002919"
     CDS             complement(559..>1390)
                     /locus_tag="ACG9CY_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4939339.1"
                     /translation="FIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGILLEAVL
                     GFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITSDRLLSV
                     IFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSLNLFVF
                     GIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIGRGLTLL
                     EAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFTYHPIE
                     "
BASE COUNT          483 a          279 c          218 g          410 t
ORIGIN      
        1 caaaaactaa tctcatcata aacattacat atatttctca aaaagttgtt ttctttcttc
       61 aaaacttact tcacgatgga cctgatgaag catccataaa taaccaaaag gatctctgaa
      121 cattgagtta gtcacaccaa agtccatcat ttctgtcatt tcttgaatct ctacacagcc
      181 atttcgtaat gctttattat gtgtgtcctc gatatcagag acgatgatat tgaaccagat
      241 cgtatttgga tgttcagcag taggagcagt caaaccaaga gacgtgtttt catccagcat
      301 atggaatctc gtgccataca aggtaaaaat gacttcatta ttgcctctag ggtaatctgt
      361 tgcctcaatc agttgtattt catcaaagat tgtttgatac agttctagtg ctttcaaact
      421 atcatttaat acaaaatcta tttccgttcc tttatgcata aagatctcct ctccttttca
      481 gtcgaacagt tactagattc ttttattata ccagttaaag aaaacgcatt caaaaaaata
      541 gcggatttac ttaccaactc attcgattgg atgataagta aatccgcgtc ccattacttc
      601 tgctacgctg ctgacaatga caaaggcact tggatcgatt tcttctatca atggtttcat
      661 tcgggaaaat tcatgatcag tcacaacgat caataaaacc tcttttgact ttcgattata
      721 cccgcctttt gcctctagca gggtaaggcc acgtccaatc tcagcactta agcgaaggcg
      781 gatgtcttcc aaataatgtt cactcatgac cataacagat ttctttcgat tgatacctgt
      841 ttcgatataa gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat aaagaaactc
      901 ttctattcca aaaacaaaca gattaagaga aacaacgaat gcgtctgtaa aaaataagcc
      961 aatcgaagta ttcaagtgga aatatttttt aaaaatgagg ggagggatcg tcgtaccgcc
     1021 gctagaagag ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg cacttccaaa
     1081 aatgacagac aacaaccgat ctgaagtaat cattgtttct ggaactattg caatgattaa
     1141 aggaaaaaac aagcttccga aaagtacttt aaaaaagggt tttttaccta aaaatagaag
     1201 agctagtacc aacataacga tattcaacac taaaataact atagctcggt caaatcctaa
     1261 aacagcttcc agtaatattc ctagaccgct gacacctcca gcagcaatat gatgaggccc
     1321 taaaaacata ttgatagcga ctcctaatat gaagagggca aaaataatct taatgtatgt
     1381 ccatataaat
//
LOCUS       DAZULG010000247         1389 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000247 DAZULG010000000
VERSION     DAZULG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.256"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..137)
                     /locus_tag="ACG9CY_002920"
     CDS             complement(<1..137)
                     /locus_tag="ACG9CY_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4939340.1"
                     /translation="MKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVPAFIIMMLI
                     D"
     gene            complement(121..>1389)
                     /locus_tag="ACG9CY_002921"
     CDS             complement(121..>1389)
                     /locus_tag="ACG9CY_002921"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4939341.1"
                     /translation="IAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQV
                     IKIFGAKVTSFKALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFI
                     TSLTTPKILAVDLLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQ
                     LKYGNETQIKNCDIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKL
                     ISGFYNVDKGQIKIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPA
                     SKVFDALRLAGCESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMD
                     EASASIDPDNEYELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHN
                     ELMAKHQVYKQLVTNYENANDWRLDNEKFL"
BASE COUNT          448 a          255 c          190 g          496 t
ORIGIN      
        1 tcgtctatca gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaag
       61 ctagaaatag atgctttaac caaatcttct gcgccctgat tagttaaagc gaatttgttt
      121 ttataaaaac ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa
      181 ttgtttatac acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt
      241 cccttctgac aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat
      301 cataataact gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt catactcatt
      361 atctggatca atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat
      421 tgctctggca atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc
      481 aattaaggta tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct
      541 tagcgcgtca aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta
      601 aatactctga ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt
      661 gtcttttgaa tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt
      721 gtagaaacct gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa
      781 tgcataaatt cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt
      841 gtatgaaaag ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc
      901 atattttagt tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata
      961 tcttgcttca aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat
     1021 tccacttaaa aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga
     1081 agttataaaa aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg
     1141 aaaaagaaca aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc
     1201 gttaattgct ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac
     1261 ttgaattccg cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata
     1321 tttttgaata aaatttgtat tctctgtcat tttctttaac aagaatacac caattactaa
     1381 catggcgat
//
LOCUS       DAZULG010000248         1386 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000248 DAZULG010000000
VERSION     DAZULG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.257"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..652
                     /locus_tag="ACG9CY_002922"
     CDS             5..652
                     /locus_tag="ACG9CY_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4939342.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            736..>1386
                     /locus_tag="ACG9CY_002923"
     CDS             736..>1386
                     /locus_tag="ACG9CY_002923"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS4939343.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEG"
BASE COUNT          477 a          243 c          285 g          381 t
ORIGIN      
        1 atcaatgaaa aaaactgtta tttatactag tttatttgca gctttgtttt tagctgcttg
       61 tcaatcaaat accaatcaag gcacatctgg gaatacgtct tccagtagcg aacagcgctc
      121 ttcacaggta tcggactcct caacaaaagc aagcacatct tcgtctagcc agacaaaaag
      181 ttctagtgaa gcaagtactc agtcagatat caaagtgtct gcagctcgtg cgatcgaatt
      241 gttccaagat aaatacccag atactgcaat tactagtttg gaattagata gtgattgggg
      301 atcttacttc tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag caagaattga
      361 tgctcaaaat gaaaaaatgg agacagagaa tccagaaact ctagatcgag atgaacaaaa
      421 tggtcaaaag aaaaaagaag atggtctgga tgttgataaa ttgatcacga ttgaggaagc
      481 agcaaaaatc gccgtcgaca aagtaggaag cggtacggca acagattggg atttggataa
      541 agatttaggt actacttatt gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt
      601 caagatcaat agccaaacag gcgaagtact gtcaactgaa caagatgatt aagaatttct
      661 tcggaaaaca agagttgatt tttcttttag tcatgataca ctagacaaaa taacttgttt
      721 gaaggagcga tttacttgag tttagaaacg acctttgcca atcatatctt tgccaatcct
      781 ttgatgaatg catctggtgt ccactgcatg actacccagg aattagatga actggctcat
      841 tcagaagcag gagcatttat cactaagagc tgtacgatca atgaacgaaa aggaaatccc
      901 gaaccacgct attttgatgt tcctttaggg agtatcaatt ctatgggact tccaaactta
      961 ggtttttctt attatctaga gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat
     1021 caaccgttat ttttttccat tgccgggatg agtgtccagg aaaacttgga aatgcttgag
     1081 aaaatagaaa aaagtgggtt taatgggatc actgaattaa atttatcttg tcccaatgtg
     1141 cctggaaaac cacaactagc ttacgatttt gaggccactt atgaaacact gaaagaagta
     1201 ttcagcatct tttctaagcc gctaggaatc aaattgcctc cttattttga ttttgctcat
     1261 tttgatcaga tggctgatat cttgaatcag tttccattga cctatgtcaa tgctatcaac
     1321 agtgtgggca atggtttgta tatcgataca gaacaagaag cagtagtcat caaaccaaaa
     1381 gaaggg
//
LOCUS       DAZULG010000249         1369 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000249 DAZULG010000000
VERSION     DAZULG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.258"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1367
                     /locus_tag="ACG9CY_002924"
     CDS             <1..1367
                     /locus_tag="ACG9CY_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939344.1"
                     /translation="FYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALT
                     IIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQ
                     IKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSI
                     IQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLF
                     QKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAA
                     KENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGES
                     WEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDV
                     NAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKK
                     NENK"
BASE COUNT          482 a          200 c          244 g          443 t
ORIGIN      
        1 gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt
       61 tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag
      121 caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata
      181 gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct
      241 taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa
      301 gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt
      361 tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag
      421 aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta
      481 tcattcaagc gttaaataac gatattacaa catacactat ggggttatta ggattggttg
      541 cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg
      601 ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa
      661 aaacaccgat aagcttgctc cagtttcctt ggcgtatctt aggtatccaa atcttttttt
      721 cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt
      781 taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt
      841 acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac
      901 gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag
      961 aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt
     1021 ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga
     1081 cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg
     1141 actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag
     1201 aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt
     1261 attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc
     1321 gatatgtgac taaaaaggag ataaacaaga agaatgaaaa taaataaaa
//
LOCUS       DAZULG010000250         1357 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000250 DAZULG010000000
VERSION     DAZULG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.259"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..>1357
                     /locus_tag="ACG9CY_002925"
     CDS             102..>1357
                     /locus_tag="ACG9CY_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4939345.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLR"
BASE COUNT          426 a          270 c          259 g          402 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      721 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      781 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      841 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      901 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      961 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
     1021 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
     1081 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag
     1141 atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata
     1201 caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca
     1261 gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg
     1321 cgtacggctt tcgcaacttt tacaatttca aactacg
//
LOCUS       DAZULG010000251         1342 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000251 DAZULG010000000
VERSION     DAZULG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.260"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..774
                     /locus_tag="ACG9CY_002926"
     CDS             91..774
                     /locus_tag="ACG9CY_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS4939346.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(811..1308)
                     /locus_tag="ACG9CY_002927"
     CDS             complement(811..1308)
                     /locus_tag="ACG9CY_002927"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4939347.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          422 a          236 c          226 g          458 t
ORIGIN      
        1 acatgttggt aaatcaacaa aaaaacgcat atagtagtct caaaactccc aagaaatgag
       61 gactatttaa caaactacga tggttacgtc gtgacaactg aacattttga cttagccaat
      121 cctgttacta aagtcgatga tatccccgat tatgaaatgt acagtcaaac aatcgatagc
      181 ttaaataagc gttttggcaa tcgtgtttta aaaggcatca aaatcggtta catcgcttct
      241 gaaaaggatc ggatcatcga ctatttagcc gataaagatt acgatttaaa attactcagt
      301 gtgcatcata atgggcaatt tgattattta gatgacgaag tcaaagatat ggatcctgct
      361 attgtgattc ctcagtattt tgcacaactt tcagaagcac tagttgttat agaagcagat
      421 gtctttgctc attttgacta tggttttcgg gtatttggtc tatccgttgc tgaatttaaa
      481 caatatgaag cgcagttttt accgattcta gatcaagtaa tcaaaaataa attggcattc
      541 gaattaaatg ctaagagcgc ttacttgtat gacaatttag cgttatatga atatgtcatc
      601 gatttatatc tttcacgtgg tggtaccttg tttagtgttg gatcagatgg gcattacctt
      661 gaacatttcc gtttccattt cgatgatctt tttgctctgt taaaagcaaa tggtgtgacg
      721 gaattagcga tttatcaaaa aggaaagcga attatggttc cattaccagt ctaaaaaaac
      781 gtcctcagca tcaaaagtga ggacgttttt ttagatttct ttttctaaaa tcggacaaat
      841 acgatcttgt tttcctaaag gaaaatgttt gatcccgatt tttttaaatc catgacgttc
      901 ataaaaacga atacctgcat gatttttttc ataaacagtc aaacgtaaat aacgtttttg
      961 tttcgtttta gcataacgat tcgcttcatc gagtaaccgt tgccctaacc ctttgttttg
     1021 ataatctttg agcagataga gccgttgaat ctctatagca tctttttcat cattgacttt
     1081 catataacca atgagtattt gatgatcctt aataccaata aatttactgg ttgactgtgc
     1141 caattctact gccaatattt cagttgtcaa ttgtgtctgt aaatatcctt gcatatcaag
     1201 tggatctgcg ccatcgataa acgattcata aaaactgaca acacttacaa cttgtaaagc
     1261 atctagatca tcataatcta aatacgctag tgtgatttga ttgttcatct aactcctcct
     1321 tttgacatag tatactataa at
//
LOCUS       DAZULG010000252         1323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000252 DAZULG010000000
VERSION     DAZULG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.261"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..1323
                     /locus_tag="ACG9CY_002928"
                     /pseudo
     CDS             41..1323
                     /locus_tag="ACG9CY_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          421 a          284 c          259 g          359 t
ORIGIN      
        1 gaataaagtc gacgaaaacc attcaaagga gtgtttcaag atgtcctatt cagagtttac
       61 caaagagtta ctagatattc tagacctaaa tcttatcttt catgaagatg cctttcgcaa
      121 agagcgaatc aatgacgaaa catgttttgt ttttgatggc acattgacct atcaaccgga
      181 ggaatgcttt catcgccact atcaaaatga acaaacgatc attaaatagg gctggaaaaa
      241 agtatccatc ttattgaatg atgtcagtaa ctacaagacc attctgcgga ttaacaaaca
      301 gcgcttcaaa tgtaaacact gtggaaagac ttttttagca gaagattcag ttagtgatcg
      361 tcgctgctcc attgctcgca gagtcaaaca agccattctt gagctattaa gcgaacctct
      421 ttctatgtct ttgatcgcac gtatgaagca catttctccg accactgtca ttcgaatact
      481 tcgcagcctt cggcctaaaa ccatttcttt gagccagctg ttaccagaag tggtgtgttt
      541 cgatgaattc aaatcagtga aaaatgtctc gggagccatg agctttgtca tgatggacgg
      601 caagacccat caactgattg atattgtgga aaatcggcaa ctcaatcctt tacgcgacta
      661 tttcttgcgc taccctataa aagtccgtga acaagtgcgt ctagtggttt cagatttcta
      721 tacaccctat cgcacattgg tgaaagagtg ttttccgaat gcccgaatcg tcgccgatcg
      781 ctttcatatt agccaacaca ttggtcgggc ttttaccaac caccgaattc aagtaatgaa
      841 gacctttaaa aaaggggatc gtcggtcgaa gcacttaaag aaatactgga aactacttca
      901 aaagaatgcg tgggaattga aaggtcagca tcgctactgg cgtccgtcat ttcgagatca
      961 tctgacagaa gcagagatag tcgatcggct attatcttat gatgattcct taaagcgagg
     1021 ctacgaagtc taccaggatt ttctttccgt tatcagaaga caagatgttc ctgaattcgt
     1081 cgcgctccta aaagaagact acaaggagtt gccagagcac taccaaccag tgttcacgac
     1141 cttcaagaaa taccaaacag aaatcaaacg agcgttgcgc gttccctatt ccaacggccc
     1201 tatagaatgt ttgaataacc atatcaaagt actaaaacga attgcctatg gctatagaaa
     1261 ctttcaaaac taccgagaac gaatcttttt atatcgagga aaatatttta agaaaactaa
     1321 gaa
//
LOCUS       DAZULG010000253         1314 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000253 DAZULG010000000
VERSION     DAZULG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.262"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1314
                     /locus_tag="ACG9CY_002929"
     CDS             82..>1314
                     /locus_tag="ACG9CY_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS4939348.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGF"
BASE COUNT          444 a          257 c          251 g          362 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg attt
//
LOCUS       DAZULG010000254         1311 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000254 DAZULG010000000
VERSION     DAZULG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.263"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..710
                     /locus_tag="ACG9CY_002930"
     CDS             186..710
                     /locus_tag="ACG9CY_002930"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4939349.1"
                     /translation="MCNDLFDTEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLT
                     HSIHGRPKSADDRSDFGHWKADIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYV
                     SEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKI
                     RIDSFENTCQNRKR"
     gene            complement(903..1142)
                     /locus_tag="ACG9CY_002931"
     CDS             complement(903..1142)
                     /locus_tag="ACG9CY_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4939350.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(1166..>1311)
                     /locus_tag="ACG9CY_002932"
     CDS             complement(1166..>1311)
                     /locus_tag="ACG9CY_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFS4939351.1"
                     /translation="APSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEM
                     MAH"
BASE COUNT          445 a          246 c          234 g          386 t
ORIGIN      
        1 cggaaaaaaa ttatgcttat cgaagaaaaa aatgcggacg ttatcacatt ctaaatgatc
       61 cgtgagtttg gtcaattgtt cgtcatttat ttgtcgattt tcagtggtcg ccagaggaaa
      121 tctcgaatcg tttgaagcat gaacatatgg acttttcaat tagttttatc acgatctaac
      181 gtgcaatttg caatgatctt ttcgatacag aacccctctc acacggcaat aaaggcctga
      241 tccgttcatt gcaccatcga ggaaaaacaa gacatacaaa aaatcatgtc gagtctcgtg
      301 gaaagatttg ccttactcat tcgatccatg gccgtccaaa atccgcagat gatagaagtg
      361 attttggtca ttggaaagcc gatattgtag ctggcaaaac tggtggtact tgtttagtga
      421 ctttgacaga cagaaaatca tgcttccttt tagcaggaaa gaaggtcccc agaaagaaag
      481 ccgcttatgt gagcgagaaa atgattgaac ttttaagcag tattccaaat gaggcggtca
      541 aaacgatcac tccagataga ggaaaagaat tctccaatca tttcgcagtt acagaagcat
      601 tgaataatat gcccttttat tttccagatc ctcatgccct tggcaacaat gaacaaatta
      661 aaatacgaat agactcattc gagaatactt gccaaaatcg aaagagatag ctacggtaga
      721 taactcaacg ataaatcaga tgattgaaaa actgaataac agacctagaa agtgtttaga
      781 ttggagaaca ccttatgaag tcttctttga taaagtgttg cgcttgattt gacaattcaa
      841 gacataataa aaagcagatc aaatccaaat aagggaaaaa tgatctactc tggtattatc
      901 aattaattgg ccaagctatt atgccgtttt gctaaacgtc tacttcttcc agtattgacc
      961 ttgataaaac aacaatcagg atcccgtttt aatactctat gaactgtgtc aatactaaca
     1021 tgtaaagttg cagcaatatc agctaatgta ctcccattta atctcatttg ctttgcaaat
     1081 cgataacgcc gataagcatc aatattcttg gaatccatag acaataatac ttttcttacc
     1141 atgtatttcc tccaaaaatt gaattttaat gagccatcat ttcttgagca atttcatgtt
     1201 tagacaatga agctggatag aaagtaggcc aattatgcaa ttcattatag agtttttctt
     1261 gactttctcc accaaagaaa atatggaaat gctctgcctt acttggtgca a
//
LOCUS       DAZULG010000255         1299 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000255 DAZULG010000000
VERSION     DAZULG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.264"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..860)
                     /locus_tag="ACG9CY_002933"
     CDS             complement(<1..860)
                     /locus_tag="ACG9CY_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFS4939352.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIG"
     gene            complement(865..1050)
                     /locus_tag="ACG9CY_002934"
     CDS             complement(865..1050)
                     /locus_tag="ACG9CY_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4939353.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          400 a          251 c          156 g          492 t
ORIGIN      
        1 ttcccaattg attgttgagt ttttttccat gtttttaaat cttcaacggt ttgtctgtct
       61 aacgaaatta tccgaatact tgatgtattt tttgtttcag caaattcata atcttgtctt
      121 gtacggatgt aaattgactt atttacgcta catctgccat ttattaagtc aaagtctttc
      181 cattgtaacg ctaagagctc ctcactcctt acacctgtaa caaaaataaa acgcatcaaa
      241 cgtttataaa attcttcata aaaatccccg tgataaacac tattatatac tttcttaaat
      301 tcatcaactg tccaaaattc tacttgagga cgctgagttt ttattttacc tatagtatct
      361 acaggatttt cttcaataat ttcaagtaca attgcccttt taaaaactac tctcaactta
      421 ttgaatatct gtttaatata atttggtgat aatggttgct caatgccctt ttttacaaca
      481 tggattttcg ataaaaactg ttgaaattcc tgtatatgaa ttggtcgaat ctttacaacc
      541 tgcatttctc caaaaaaatc caatgcacgt tgtaaaatat gatctgtttt catatatgtt
      601 ttttctacag ttcccaactt ataccaagga aaaaaatact ctcgataaaa atcatgaaac
      661 gttattcctt gcagataaat tccttgtgaa tcagaaaaac tttgttttaa ctcttcgagt
      721 ccccttctag cttctccttg agttttgaag cctcttctgg tcacttgaat tctttttcca
      781 aagcgatctt gtcctaaagt tgcagaaaaa aaccaagtgc ctctttttct gtctttataa
      841 acattcgcaa ttttcctcat tttactattc ctcccaagaa atatggagaa cttcttctaa
      901 aattgaaata ggaattcttt ttatttttga attacgataa aaaaagtatc ctttctcaac
      961 taaaagttct ttacattcct tgaagatttt attagcatag tactgagaaa aacctagatt
     1021 aatcaaatct tttcgagtag ctgttttcat tatagcacct tccttacttt ttttcattta
     1081 tttctatcag ctgcttttgt caaaagcaca taggaatttc acctctcccc aagtttacgg
     1141 cgagccttac ggaagtatca ttatcccaac tttaaatcat cgctttttat tgactataaa
     1201 aattatttta gaatatttat cgctccttct ttacagcaag aaatcatggc gcaactaaaa
     1261 tatgctcctt aaagtgttaa cgtactgata gaatctatt
//
LOCUS       DAZULG010000256         1282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000256 DAZULG010000000
VERSION     DAZULG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.265"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..935
                     /gene="scrK"
                     /locus_tag="ACG9CY_002935"
     CDS             63..935
                     /gene="scrK"
                     /locus_tag="ACG9CY_002935"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4939354.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          387 a          231 c          305 g          359 t
ORIGIN      
        1 ttaaatatga tatatagtca tttatttagt gatttcattc taaaataaag ggagaaatgt
       61 ctatgctata cggtgcaatt gaagcaggcg gcacaaaatt tgtgtgtgcc attggcgatg
      121 aagaaatgac gatcaaggaa agagtgagct ttccgacaac gacacctgag gaaacaatgc
      181 ctttagtcat tgacttcttc aaacaatacc aagcagattt agcagggatt gggattggct
      241 catttgggcc tatcgatatc catcgcgatt ccgccactta tgggtacatt acctcgaccc
      301 cgaaattggc atggcaaaat tttgatttca tcggtaccat gaaaaaagaa tttccgatcc
      361 cgatttcttg gacaacagat gtcaatgcgg ctgcctatgg tgagtatgtc ttcggcagtg
      421 gcaaaggatt gagcagtgtc gtttactata cgatcggtac gggtgttggc ggcggtgcct
      481 tacaagaagg gcgctttatc gaagggttta gtcatccaga aatggggcat atgctcgtcg
      541 tccctcatcc aaaagatgac tttgctggca gctgtccatt tcatggcaac tgtttagaag
      601 ggatggccgc tggacccgcc atcgaaaacc gcttagggaa aaaaggacaa gaggttgcag
      661 aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc tacaatacaa
      721 ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa caacgccata
      781 tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc gaaacacctg
      841 agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg ctcggttgtt
      901 tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct tcgacattta
      961 gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc agagtgctta
     1021 actggttttt gttttacttg gaagatcaag ccttgaatga gataaattct tgctcgaaag
     1081 ttatggaaat ttcgatagcc gtaggccact tgtattgtgc aatagttttt tacaaaaaaa
     1141 agggggtgcg gatatttttt tggacctttt taacaaatag gttctgttgc aaagttttaa
     1201 atctactatc agataaggta gaataataga aaaagatagc aggaggaatg acgatgagtc
     1261 attttaaagg aaagcaattt ca
//
LOCUS       DAZULG010000257         1268 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000257 DAZULG010000000
VERSION     DAZULG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.266"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..>1268
                     /locus_tag="ACG9CY_002936"
     CDS             109..>1268
                     /locus_tag="ACG9CY_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4939355.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRK"
BASE COUNT          439 a          178 c          247 g          404 t
ORIGIN      
        1 ccttctagga gtgttctcga caaacttata ctagttggat atcgattatg actcattttt
       61 atgtgttgca cttaaagtgt aaattcaagg tatagaaaga ggttccctat gaaaaaaata
      121 cctattacaa tcttaactgg ctatcttggt tcaggaaaaa cgatacttct aaatcatatt
      181 ctgtacggtg atcacggaaa ggaaattgct gtcattgtaa atgacatagg ggaagtaaat
      241 gttgatgggg tattagtcga caagaaaggt tttaaaagaa ctggagaaaa acttgtttcg
      301 atgccgaatg gatgcatttg ttgtacatta cgcgaagatt tgattgttga actagatcaa
      361 ttagtgcaat tttctgactt gaatcaaatt gttattgaag cttcaggaat cagcaaacca
      421 attccaatta ttcaagcaat tatattagct aaaaaccctt tagacattga tttgacccaa
      481 cgattagaga ttggtgccat tattatagta gtggatgtaa aacgtttttg ggataatttc
      541 gaaacgggtg aaaagattca ggaaagaaaa gctgaaggta atgtagttga agaagaacag
      601 gatattcgtg gcttgttaat tgatcaattc gagtgttgta acatattact tttaaataat
      661 agtgacttag tgtcaaccaa gacgatttac aaaattaacg gtctaattgc taagttacag
      721 tccaatgcca aaataattag gacaattaaa ggtgaagtag acttatcgaa aattctaaat
      781 acaaatcttt ttaatataga tgagacttcc tattcagtgg gctggttcaa agagttagag
      841 cttggctatc aaaatcatat tcctgaaaca gaagaatatg gtatctcaag ttttatctat
      901 cgaaatcggt tactccttga cgcacaaaaa tttgcccatc ttacggataa aaattttcct
      961 ggcaatatta caagagcaaa agggtttctt tggtttgccc aacatagtga tttttcgacg
     1021 atctttgaaa catcgggtag ctataaaagt attgaatcat taaattattg ggtagctagt
     1081 ttacctaaaa ttgagtgtca aatgattata caaaaaaatc ccgaattgaa ggaagcctgg
     1141 catccgttgt ttggtgaacg cattaataaa ctagttttta ttggtatgaa gatggatcaa
     1201 caatgtatta aggaacaact tgagggttgt ttaactgatt ctcttaatgc agataatttc
     1261 cgaaaaat
//
LOCUS       DAZULG010000258         1247 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000258 DAZULG010000000
VERSION     DAZULG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.267"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..1129)
                     /locus_tag="ACG9CY_002937"
     CDS             complement(35..1129)
                     /locus_tag="ACG9CY_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS4939356.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          358 a          266 c          193 g          430 t
ORIGIN      
        1 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
       61 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      121 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      181 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      241 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      301 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      361 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      421 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      481 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      541 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      601 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      661 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      721 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
      781 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
      841 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
      901 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
      961 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1021 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1081 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1141 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1201 tacacctttt ctttttcttt taaaataaat gataaattcc aataatt
//
LOCUS       DAZULG010000259         1204 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000259 DAZULG010000000
VERSION     DAZULG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.269"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            220..630
                     /locus_tag="ACG9CY_002938"
     CDS             220..630
                     /locus_tag="ACG9CY_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4939357.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            687..1127
                     /locus_tag="ACG9CY_002939"
     CDS             687..1127
                     /locus_tag="ACG9CY_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4939358.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
BASE COUNT          396 a          201 c          232 g          375 t
ORIGIN      
        1 ttttgaagaa agaaaacaac tttttgagaa atatatgtaa tgtttatgat gagattattt
       61 tttgtctctg atattttttg tatatgtttg ctattcgaga aagcgcgtct gtttcatgtg
      121 ctttctctta tttatcttat gccaatccat ttctttgctt tccacaatta tattgtgtaa
      181 aattcaattg tataaaatat aattaaaggt ggtaatttta tgaaaaaaat gtattctacg
      241 aaaatgatca acacaggcgg acgctctgga gaagttcata atcccgatcg tacattcgaa
      301 ttgaatattg cagctcctgg taaacgcgtc gaaaatgcaa ccaaccctga acaattgttt
      361 gcagctggtt tcagtgcatg ttttaacagt gcattagaat tgatcaaaca gcaaaaaaat
      421 atctctgggg cttctactgt cagtgcacaa gtttcccttt attcagaaag tgaaacgagc
      481 tttgtcttag gtgtagagat tgaaggatct atcgaaggtc tttctctgga agaaacgcaa
      541 gaattattag aggcagcgca taaagtgtgt ccgtattcaa aagctacagc tggaaatata
      601 gaagttactc tgaaagcagt tagtgaatag acttttttgt gtaaatccgc tatgatacac
      661 tatagtaaaa cagcggaggt ggaaaaatgg aagatctata tttagttaat caagtatgtt
      721 ttcctttata tgccgcctct aaggaaatca ttagacacta cacttccctc ctcaagccgc
      781 tgaatcttac ctatacccag tatcttgtga tgttagtttt atgggaaaag aaaaagactt
      841 cagtgaaaga gctaggaaat gatttgtgtc tggattctgg tacgctgact cccttactaa
      901 aaaagttagc agaaaaaaat tatattaatc gtcagcgtga aacgggtgat gaaagaatcg
      961 taaatgttac gctgactcct caaggtgctg cgttgcaaaa acaagcagaa ggtattccac
     1021 aaaagatcaa gtcttatctt ccacttgaac aggaagaatt ggatacactg caacatctga
     1081 cccgaaaaat gctagataat tttcatcaag accagaaaaa gggctgaaac tgctagatga
     1141 acgattttat gaggaaaacc aactcataag ataacgtcat ttagcggttt ttctttttgc
     1201 tctt
//
LOCUS       DAZULG010000260         1197 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000260 DAZULG010000000
VERSION     DAZULG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.270"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            192..461
                     /gene="rpsN"
                     /locus_tag="ACG9CY_002940"
     CDS             192..461
                     /gene="rpsN"
                     /locus_tag="ACG9CY_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4939359.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            476..655
                     /gene="rpmF"
                     /locus_tag="ACG9CY_002941"
     CDS             476..655
                     /gene="rpmF"
                     /locus_tag="ACG9CY_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4939360.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            687..1064
                     /locus_tag="ACG9CY_002942"
     CDS             687..1064
                     /locus_tag="ACG9CY_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939361.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1085..>1197
                     /gene="rpmG"
                     /locus_tag="ACG9CY_002943"
     CDS             1085..>1197
                     /gene="rpmG"
                     /locus_tag="ACG9CY_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4939362.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSP"
BASE COUNT          439 a          167 c          251 g          340 t
ORIGIN      
        1 attcttttaa atgatttgat tatctctatg taccgataaa ttcgttttca aatagttatc
       61 tattcatatg agttttattt catgttattt attgttgaca agagcaactc ttgttggtaa
      121 agtatcagtg tcaaaacgta acgattacga tttgaggtgt ctaaatcaaa ataaaaggag
      181 tataaaggta tatggcaaaa aagtcgaaaa ttgctaaagc aaagaaacaa atggcgatga
      241 ttgaaaagta tgcagataaa cgtcaggaat taaaagccgc aggagatcgc accgctctcg
      301 ctaaattgcc aagagattca aatccgaatc gtctgaggct tcgtgatcag acggatggtc
      361 gtccaagagg gtatatgcga aagttcggta tgtcccgtat taagtttaga gaacttgccc
      421 atcaagggtt gattcctgga gtaaaaaaag caagttggta aaaggaggaa atatcatggc
      481 agtaccagca agaaaaacat ctaaggcgaa gaaacggtta agaagaactc atcaaactgt
      541 agtaaaacca gaaatttcat ttgacgaagc gaatggtgat tatagacgta gccatcatgt
      601 atctttaaaa gggtactata atggaaagaa agtaattaaa ggagcttcaa aatagtttaa
      661 tatataactt ttaggaggga atcaaaatgg aaattattta cccaccttta gttgaagaag
      721 gattaaagta ttatcttgaa acgacccagc aatcgctaga taaatgcact ttttatcgtt
      781 cgatggtaga gagaggaatt attactgaaa cgggtttacc gacccaacag gcaattgaaa
      841 atggattagt caaagattac tatgaggacc aaggcttatc atttgatgat tttttaagaa
      901 tctatccaat ttttgaagag tatgacgagg agttatttca atgtatcgat ggttattggg
      961 agatacctgt tgatatgaaa gaaaatttag tttcacaatt ggaatctggc gagttaactt
     1021 ttgaagatgc acaacaaatt caagcatatc ttgaagatcg ataagtattg aaggaggaag
     1081 atttatgcgt caaaatatta ttttggaatg tgtcgaaact ggagaacgtc tgtatctcac
     1141 cagcaaaaat aaacggaata accctgaacg gttagaatta aaaaagtatt cgccaaa
//
LOCUS       DAZULG010000261         1195 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000261 DAZULG010000000
VERSION     DAZULG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.271"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG9CY_002944"
     CDS             <1..1076
                     /locus_tag="ACG9CY_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4939363.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZULG010000262         1177 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000262 DAZULG010000000
VERSION     DAZULG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.272"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1177)
                     /locus_tag="ACG9CY_002945"
     CDS             complement(<1..>1177)
                     /locus_tag="ACG9CY_002945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939364.1"
                     /translation="LTKFGFIDDINESHSTRAQSLKEFLDPYIIVCTPKIKRNHEQAR
                     NYYQGDDLELIPKPKSEVFKNLIKENESLVPIPVFSINNSDSVIKDETSFENQLLNGK
                     LLGKIINFSKDNEDYPLNVFWENTDGSRVLFGEIIGQNNSMYGVAYRIEKDNFYKKFI
                     DEGSKNYVGYDDFINQTKDIIFVPYLLLQKIIETKKDINEGIENISDSKISPVSSIIT
                     EQTQIDISELEDKHAIQQNDHFSEHNEFIERFNQLTIRSKLHYSKRDLWNFHTAMLGD
                     SLVVLAGLSGTGKSQLVTSYAKALQLSDTQIKFISVRPFWEDDSDLLGYADTVNSVYR
                     PGDSGLIDALLEASRHKENIYMVCFDEMNLARVEHYFSQFLSVLEMEPNTRKIKLYNE
                     "
BASE COUNT          370 a          189 c          167 g          451 t
ORIGIN      
        1 cttcgttata cagtttgatt ttcctagtat ttggctccat ttctaaaact gaaagaaatt
       61 gggagaaata atgttcaacc cgagctaaat tcatctcatc aaaacaaacc atataaatat
      121 tttctttatg tcttgatgct tctaatagtg catcaattag accgctatct cctggacgat
      181 agacagaatt tacagtatca gcatagccca ataaatcaga atcatcctcc caaaagggtc
      241 tcacagaaat aaactttatt tgtgtatctg ataattgaag tgcttttgca taggatgtta
      301 ctaattgtga tttaccggta ccacttagtc cagctaatac caccaaacta tcacccaaca
      361 tagcagtatg aaaattccat aagtctcgtt ttgagtaatg taatttacta cgaattgtga
      421 gctgattaaa tctttctata aattcattat gttcagagaa gtgatcattt tgttgaatag
      481 cgtgtttatc ttctaactca gatatatcta tttgcgtttg ttcagtaata attgaactta
      541 ctggagatat tttggaatca gagatatttt ctatgccttc gttaatatct tttttagttt
      601 ctataatttt ttgaagtaat agatagggca caaaaattat atcttttgtt tgatttataa
      661 aatcatcgta acctacatag tttttactac cttcatcaat aaactttttg taaaaattat
      721 ctttttctat cctatatgct acaccgtaca ttgaattgtt ttgtccgatt atttcaccaa
      781 ataaaactct tgatccatca gtgttctccc aaaaaacatt taaagggtaa tcttcattgt
      841 cttttgagaa atttataatt tttccgagta gtttgccgtt tagtaattgg ttttcaaaac
      901 ttgtctcgtc tttgattaca gaatcgctgt tatttataga aaagaccggt ataggtacta
      961 atgattcatt ctcttttatt aaatttttaa aaacttcaga ctttggtttg gggattaatt
     1021 ctaaatcatc accttgatag taatttcttg cttgttcatg atttctttta atttttggtg
     1081 tacaaacgat aatataagga tctaaaaatt ctttaagtga ttgggcacga gtactatgag
     1141 attcgtttat atcatcaata aacccaaatt tagtaag
//
LOCUS       DAZULG010000263         1164 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000263 DAZULG010000000
VERSION     DAZULG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.273"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..601
                     /locus_tag="ACG9CY_002946"
     CDS             47..601
                     /locus_tag="ACG9CY_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4939365.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            657..>1164
                     /locus_tag="ACG9CY_002947"
     CDS             657..>1164
                     /locus_tag="ACG9CY_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939366.1"
                     /translation="MKITEFSEVFKTFSNIYNRIPEIEQNVVVDNDYYIENDFNKPRK
                     YDNLYNFIHNRTTINNNSNTRVLINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAI
                     NNGNLPSNKKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIF
                     ASIHDYIGL"
BASE COUNT          476 a          118 c          213 g          357 t
ORIGIN      
        1 tatatataat catgaactca gaatattttt aagaagggaa acaatagtga gctataatgt
       61 acaaccgtta aggacaaatg aagagataca agattttcag ttttggctaa gaagaacaag
      121 taatccagaa agagatagtt ttttattttt gtttggaatc aataatgggt tacgtatgtc
      181 agatattata ggattaaaag ttggagacat acgaggtaaa agtaagccta ttattgttga
      241 acataaaaca ggtaagcgaa aaccgatttt tatagataat ttgagagaag agattttatt
      301 atatactgaa ggtaaagaag aaaatgattg gttatttcca agtcggcaac aaggtcgaca
      361 tataacaagg gatcgtgtct atcagatata tgcagatata gcagaaaaat taggaagaga
      421 tgatatagga acacatacat taagaaaaac ttttgggtat cactactaca agaaaactag
      481 agacattgct accttaatgt ttatttttaa tcatagctcg caagcaatta caaaaaggta
      541 cattggtata actgaagatg aaataggggc atcgttacgt ggttttaaat tgggaggcta
      601 attgagatat taggatatat tttttgtgta taatgaaaac ttagaaggtg agattaatga
      661 agataactga attttcggaa gtatttaaaa cgtttagtaa catttacaat agaataccag
      721 agatagaaca aaatgttgta gttgataatg attattatat agagaatgat tttaataaac
      781 caagaaaata tgacaaccta tacaatttta tacacaatag aacaacaata aataacaaca
      841 gtaacactcg agttttgatt aattcagcat attttcatat taaatattta aagaaatatg
      901 agtatgaaga agttgtatta gattatgggc taaatagaca gtcattttta atttatgcaa
      961 taaataacgg aaatttgcca agcaataaaa agaacataat tattgaaaca tatgcctatc
     1021 tggagggggg atctaaaaaa ctaattaagt catgtaatat agagtatcaa ttatcagtag
     1081 gagaaattaa atgtgttttc gaagaagatt tagaacttga cttaaaaaaa atttttgcca
     1141 gcattcatga ttatataggt ttga
//
LOCUS       DAZULG010000264         1153 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000264 DAZULG010000000
VERSION     DAZULG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.274"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..114
                     /locus_tag="ACG9CY_002948"
     CDS             <1..114
                     /locus_tag="ACG9CY_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4939367.1"
                     /translation="ILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            119..1066
                     /locus_tag="ACG9CY_002949"
     CDS             119..1066
                     /locus_tag="ACG9CY_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4939368.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          371 a          170 c          245 g          367 t
ORIGIN      
        1 attttagata ttgatagtga agaagatttt aaatggttag aatttttgtt tcctaaattt
       61 ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag gtaaaaggat
      121 gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag ccaatcattt
      181 aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag tagaaaatct
      241 tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat ccttgatgga
      301 agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag ttgctagtgt
      361 ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa gtgtattaat
      421 gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc
      481 agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg tgattcgccc
      541 attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag attattgtca
      601 tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa
      661 tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata agaaacaaat
      721 cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta gagactttgt
      781 ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag ctgcactggg
      841 gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga ttcatgcaat
      901 caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt cgggtgatat
      961 ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac cgaaatatgg
     1021 tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca tactaagata
     1081 agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt gtaaaatcaa
     1141 atctgggata gaa
//
LOCUS       DAZULG010000265         1139 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000265 DAZULG010000000
VERSION     DAZULG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.275"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..125
                     /locus_tag="ACG9CY_002950"
     tRNA            55..125
                     /locus_tag="ACG9CY_002950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87..89,aa:Gly,seq:ccc)
     gene            486..>1139
                     /locus_tag="ACG9CY_002951"
     CDS             486..>1139
                     /locus_tag="ACG9CY_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4939369.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     "
BASE COUNT          385 a          189 c          247 g          318 t
ORIGIN      
        1 aagaataaaa aaacttgagg aaggaataaa agaggcgtat actgtttttg tattgcgggt
       61 gtagtttagt ggtaaaatcc cagcttccca agctgatgtc gcgggttcga ttcccgtcac
      121 ccgcttaaaa taggttgtga aaaagctgct ctgcaacaag taataagaac agccaagcaa
      181 aaacaacttc ttatgttttt aacttggatg gacttattac cgcagatgca agcttttgcg
      241 caccgtttac caagcggttg tgaaatcagt gtattgtctt gagtatgaag aaaatttttg
      301 aataataata aataccttac agatagagtg gtgactgatt agagacgagg aatccgtctc
      361 ttttttgtat ttttaagcca tcttaaaaca ggttttattt tttgctgttt ctatttcttt
      421 ctagtattta tttatagggg aacatatgtt aagatttagg gagctgttag taaaggagta
      481 aagaaatgag caaaaaaaca accaaggcga tcgtggggat cgtcgctgct gtgattgcag
      541 gggcattcgg tgtttccacc acacaaaaag attcagatat ctttcagcag attacaacta
      601 tttttgattc cacaccacaa gcttctcaag tagcggatgt gccagaatat gatggaaaac
      661 atcaagaaat agaaatcaat gggaatcagc cggaatttac aaaagaggat cttagccttt
      721 ccaaaggaac atgggaaagc tattcaaata ttgataaatt gaacagagta ggacaagcca
      781 atgctatgct tggaaaagat ttgttcccta ctgaaaagag agagcctttg tatattgacc
      841 cgacaggttg gaaacaaaag aaattaagtg acgggcaatg gttatataat cgcagccatt
      901 taattggttt ccagctgact ggtcaaaata acaacattaa aaacttgatg acagggacac
      961 gttcgttgaa tgctccatac atgcttgctc acgaaaatga tattgcggca tatatcaaag
     1021 aaacaaatca tcatgtacgc taccgtgtca ctcctcattt tgaggggaat gaactggtag
     1081 ccaggggagt gcaattggaa gcagaaagca ttgaagacaa aaaaattgaa ttcaatgtc
//
LOCUS       DAZULG010000266         1117 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000266 DAZULG010000000
VERSION     DAZULG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.276"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..1079
                     /locus_tag="ACG9CY_002952"
     CDS             39..1079
                     /locus_tag="ACG9CY_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4939370.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          459 a          168 c          190 g          300 t
ORIGIN      
        1 ttacataaat aaaacaacgt gaaagtagag gatctacaat gactgatttt aaatttttta
       61 aggcggatag agtttacaac gaattatttt atcaatttcc aaaagtcttt attgtttctg
      121 acgaatacaa aaaaatgaaa gattcaacta agattgccta tatgctttta aaagcaagat
      181 tagagatcgc aatcagcaaa cggcaaatcg atgaagaagg taatgtttat tttacttata
      241 cgacaaatga actatgtaga gtattaaact gccaaaaaca aaaagcgata gcaatcaaaa
      301 aagagttgga atcctttggt ttattattac aaaagcagat gggatttaac aaacagttag
      361 ggaaaaataa tcctaataga ctatatctag cagaattaaa agtctcagaa aatgatatct
      421 acttactcga aaaatttgat agagagaata gggaaaacgt tgataaatca gagggtatga
      481 aaatcatacc caccctcgac gaaaaatcag acgctgaatc ccttggggct caagagggta
      541 tgaaaatcat accgtgccaa aacgttgata aatcagaggg tatgaaaatc ataccagaac
      601 ttaataataa tatattagac actaatagac acaatataga cactgaaaaa gaccgcctac
      661 aagatcaatt gttgttagac aattttgaga caattatgac aaacgacagc attgctacgt
      721 ttgtccctga acgagtatta aatttgataa aaacattttc ttcaagttac agtgaagctc
      781 aaaaaaccgt ccagactatt cataatgcaa agaaaaaagc tgaaatagaa agtgatattt
      841 cgatagtttt tgaagaactc gatagttatt atgtcaatgc agaacaagaa ttatacacga
      901 cactgttaaa agcctatcaa aaattaaaaa ccgaaaaagt cgaaaatatc cagaacctga
      961 tttttgtcta tgtaaaaaat tggtttatcg aaaaaccaat agctgctaaa gtatcaagtg
     1021 aaaaacgttt gaattatgca agctccccaa gcactattac gaaagactgg ttagagtgaa
     1081 actttactga caaagtgatt gttgcttcat ggagtaa
//
LOCUS       DAZULG010000267         1110 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000267 DAZULG010000000
VERSION     DAZULG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.277"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG9CY_002953"
     CDS             complement(54..788)
                     /locus_tag="ACG9CY_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4939371.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          392 a          218 c          201 g          299 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg
//
LOCUS       DAZULG010000268         1102 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000268 DAZULG010000000
VERSION     DAZULG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.278"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(257..961)
                     /locus_tag="ACG9CY_002954"
     CDS             complement(257..961)
                     /locus_tag="ACG9CY_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939372.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          321 a          191 c          199 g          391 t
ORIGIN      
        1 tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa
       61 tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata
      121 ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt
      181 tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt
      241 atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag
      301 gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat
      361 tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa
      421 taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg
      481 aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt
      541 ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg
      601 tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca
      661 cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa
      721 ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat
      781 ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt
      841 gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc
      901 ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca
      961 tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag
     1021 gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc
     1081 cgttttttga aagaaaaata aa
//
LOCUS       DAZULG010000269         1097 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000269 DAZULG010000000
VERSION     DAZULG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.279"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(72..>1097)
                     /locus_tag="ACG9CY_002955"
     CDS             complement(72..>1097)
                     /locus_tag="ACG9CY_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS4939373.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          357 a          181 c          164 g          395 t
ORIGIN      
        1 aagttttgtc tcgcttaatt tgacaaataa tatttttaga aaaagcagtc ggactttcta
       61 aaagaagttc actattcgat cctgtcgatg atttcttcag gaaaatcgtc tatttgcttg
      121 tcaattttgc tgtctttgta tttctcttct tggatagtca acaaaatctt tgtaagattc
      181 ccccagtcta ctgctcttgg cggatcgagc caaaccacta cttgagatgc actttcttca
      241 tatagattat tcctattcgt gattataaca tccgggaact caggctttcc tgcctctatc
      301 acttccgtgt tcagattaat gtacaagctt aaatttttct tgataaattg attataggca
      361 gcaccataag aaaaatcgat gatgatcttt actggttcag aaatcagttg aatgggtatg
      421 tgattgatca ataataataa ataactgaaa aaaagaaaaa atttatcttg aagcaactct
      481 ttgcgttttg acgttagatc atttatatac tctatcaaat agcaataaaa tgctggatag
      541 ttttctctaa aaaaagaaac atcaagtgga aattcatcag ctttattaat agtgtaatgc
      601 aaaagtttaa aatgtatttt ggttaactct cttcggatca attggctcgt ctcttctcgc
      661 aacagattaa actgattttc aaaactttca atgaatcgtt cattcaacca ttcgattgtt
      721 tcatcttcat tttttctcaa gtatttttga tccaatttat catgaagata aagccaagag
      781 agtgtcttat tgataaaaat aacggatttt tctcctaata ctccttttaa gtggtaaaat
      841 cctatatccg aaaaaaatag cgactgattg ctagcaatat ctaaagaatc agcttgttca
      901 gccaacaggt tgattgctaa aaagtggaat aatttctttt tttcataagc agacagagga
      961 taatactgtt ttaaatcttt taacattttt tctgttttta taattatagg tagcgtatac
     1021 aattccacaa aacttatctc tgttgagtta aaaagattat ataaaaaaag ccgaatgttt
     1081 ttaggattcc ctgtcaa
//
LOCUS       DAZULG010000270         1081 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000270 DAZULG010000000
VERSION     DAZULG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.280"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..>1081)
                     /locus_tag="ACG9CY_002956"
     CDS             complement(64..>1081)
                     /locus_tag="ACG9CY_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS4939374.1"
                     /translation="AALISIYQFIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNV
                     GAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVL
                     FLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKV
                     EVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFY
                     GNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSL
                     LKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYL
                     KKEN"
BASE COUNT          422 a          182 c          135 g          342 t
ORIGIN      
        1 tgataaacat ttaatatatt aatcaaatgt aaaggagtcc aagcaatgaa agctgtcttc
       61 atattaattc tcctttttta aatacaagta agaaatgctt cccaaataat accagaaaac
      121 aattgaaatg acatcgcgct tattgaaaag tacatgagcc tccaccattt catctataaa
      181 aatagatagt ataaaaatta aaacaatacc aagaaacgca taattcactt tacttctaaa
      241 aaatgttttg agtaaactaa agatatttaa cacatagaaa gcatagatac atataaaagc
      301 aattatacca cttgatacaa gtacttgaag aaatccatta tgcatgtttc catgtgctct
      361 ttttagttca ttcacatctg tagtatcaag atctactttt ttcaaaaatt ttgcatcgct
      421 attcccatag aaatcggtct ctccataacc aaataacaaa gttggtctta taactttaag
      481 cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg gagttatcac tagaatgctg
      541 taatattact tctttttctg gaagtggagt tttatctgat tcatttgact ctataacttc
      601 tacttttcta tcaatcgata caggaattgt tgctaaaaaa tccttagcat aattagtacc
      661 aaaagaaaaa tataccaata gaactaataa tatgcttacc atccatttga tttttacagg
      721 tagtacttct ctaatgctct ttctaaattt tgaattggca aacaacaaaa gaaataatac
      781 taaaaaagaa caaattacta cctgggcacc tctagacgaa gaaagtgtaa tataaaatat
      841 ttgtacgata aaattcatca tataaaaaac acgaatcgtt tttgttattt ttgaaattaa
      901 tacactaatt aaacttaaaa taatagaagt atatccaaac atagctccaa catttggact
      961 tgtatagaca ccaaataacc gatgttcaat aaaaccttgt cttgcaagta atcctggcgt
     1021 tcctgttggg acatgataag aaatcaaaaa aataaattga tagatagata ttagcgcagc
     1081 t
//
LOCUS       DAZULG010000271         1043 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000271 DAZULG010000000
VERSION     DAZULG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.281"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACG9CY_002957"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACG9CY_002957"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS4939375.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          374 a          132 c          182 g          355 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg taggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aac
//
LOCUS       DAZULG010000272         1041 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000272 DAZULG010000000
VERSION     DAZULG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.282"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..354
                     /locus_tag="ACG9CY_002958"
     CDS             16..354
                     /locus_tag="ACG9CY_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS4939376.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            341..520
                     /locus_tag="ACG9CY_002959"
     CDS             341..520
                     /locus_tag="ACG9CY_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939377.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(563..>1041)
                     /locus_tag="ACG9CY_002960"
     CDS             complement(563..>1041)
                     /locus_tag="ACG9CY_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939378.1"
                     /translation="QWMSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKV
                     IISNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPK
                     SAISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT          355 a          143 c          211 g          332 t
ORIGIN      
        1 agggaggaac agctaatgca atatctagaa gcaaaaatcc caattccaga aggctatgta
       61 attatctccc aagtggatta tgaggagtta aaaaaagctg atgatactgg tagatggatg
      121 acgttgccag aagtgctaga acggattaac agaaaatatg attggtttac ttctagagtt
      181 ttaaagaacc caagatatag aaatattatc gatatcgaga aaaacaaaaa tggatttgtc
      241 tattatccag ttgaaggaag agacacatat ctatttttaa gaagtaaaac acttgaattt
      301 ttagaaacaa atttttcgga aatcttaagg aggcaagcgg atgggaaaat ttaatagagc
      361 gctagtgttc agcgcaccgc taatcatcta cgctttagga ctttggggaa gcaggcaagc
      421 gttgatagga acgattgttt acatggtttg gatttttatg gggcttgatg aagctgaagc
      481 tgagtacaga gcgaaaaagc caaccgaggg ggctgactaa aaatgaatag aaaagagaaa
      541 ctagaatgta tattactatt actcagttta attctttcac taatttctct attgggaagt
      601 ttgtattttt gatatcaaca attttgtgga tttgggttct atttagagta aatatcattt
      661 ttgtttggtg attaaggagt ttattaaaat taattttccc tacttcaaat gcaaggtaac
      721 aagatatagc acttttagga ggaatattta gcggtacagc ttcagagtat attctattat
      781 catcgactga tcctccttta gcaattaatt tcttaaatgg agtagctgac catctgcggt
      841 ttaagttagc ggatgaaagt tctaaattaa ctaacatagc tggttctgtt gaatagttag
      901 aaatgattac ttttgtataa atcatatctg cagcgaagta agaagcatta agttcaactt
      961 ctagttgagg gcgcattcct tttattttta tccctgttaa aaacgtactc aatataaaac
     1021 ttattattga catccattga a
//
LOCUS       DAZULG010000273         1018 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000273 DAZULG010000000
VERSION     DAZULG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.283"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..937
                     /locus_tag="ACG9CY_002961"
     CDS             161..937
                     /locus_tag="ACG9CY_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4939379.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
BASE COUNT          400 a          155 c          190 g          273 t
ORIGIN      
        1 tgatatgtaa atgcagaccg ccaagaaagc tttagaatca atgtttttat gtcttattta
       61 attatgctct tagtatagag tattactcag atactgtcaa ttgaatttag aaaaaagttg
      121 ttaaatttgt ttacgaatat aaaagaaagg aatgagaaat atgaacacac cgcaaatttt
      181 taatttcgaa caaaacgaag ttcggacaat tttagtaaat gatgaaccat attttgtagg
      241 caaagacgtt gcgagtgttt tgggttattc aaacactaaa gatgctttgt cgcggcatgt
      301 agatttggaa gataagatgg ggtcgcgaat cacgacctca ggtcaatcaa gagagatgac
      361 aatcatcaac gaatctggtt tgtacagttt aatcttaaaa tcaaaacttc cctctgccaa
      421 aaaatttaaa cgttgggtaa caagtgaagt gttgccagca attagaaaac atggaggtta
      481 tctaactcca gaaaaagtag aagaagcttt gcttaatcca gatacaatca ttcaattagc
      541 aactcaacta aaagaagaaa gaactggaag attaatcgca gaacaaaaga ttgcagagta
      601 cgaacctaaa atctcctatt tagatagcat attatcttct acagattcag taacaattag
      661 tcagattgca gcagattatg ggatgtctcc acaacagatg aataaattac ttcataaact
      721 aggtattcag aaaaaagtcg gtaaccaatg gttattgtgc aaaaaacaca tgaaccaagg
      781 atacacaaaa tctcatacaa ctgagatccc gaaagccgat ggtggcacta aaattgtaat
      841 gaataccaaa tggacacaga aagggcgtct atttatctac gaattactaa aaaaagaagg
      901 atattaccct caaatggatt tagaggaaat tggttagaaa ggagttttag tatgactgac
      961 attgcagaaa tcactcaacg agatagagaa aaaatcaaag aatatgtcga aagttcaa
//
LOCUS       DAZULG010000274         1011 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000274 DAZULG010000000
VERSION     DAZULG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.285"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..>1011
                     /locus_tag="ACG9CY_002962"
     CDS             154..>1011
                     /locus_tag="ACG9CY_002962"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFS4939380.1"
                     /translation="MTFFPAVMLYWMTKNLIVSYIIYVWLLNLCTTLVAYHYGERFLK
                     QKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFYAYQIISGKEEKYPSV
                     KLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFIAIFKKLFSAAGMTLV
                     LTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDLTTYSMGLVGLLSL
                     CIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLLQFPWRILSLQIIF
                     SSLILTMIFF"
BASE COUNT          303 a          167 c          164 g          377 t
ORIGIN      
        1 atctttttca aaaatacaca gagtatttat accactggag atctttcttt tcacttatct
       61 cgaatcaagg ggttatcgac tatttttacc agtccgatta attatgagac attcaattat
      121 actggatatg gtgtaaatta tttttatccc tttctgactt tttttccagc tgtaatgtta
      181 tattggatga caaaaaattt gattgtaagc tatattattt acgtttggtt attgaacttg
      241 tgtacaacac tcgtagctta tcattatggc gagcggtttt taaaacaaaa aaaagcggca
      301 tttttattta gttgtctcta tattttctct gcttatcgga cagtagacat ctactatcgt
      361 tccgcaattg ccgaagctat cgcaataaca cttgttatcc ctgtattatt ttatgcttat
      421 caaatcatct ctggaaaaga agaaaagtat ccgtctgtaa agctagcatt gagtatgagt
      481 ttgctagtgt acagccatgt actatcaaca ttaatgagta cagcattaat tatcattttt
      541 atttttattc gtttggtttc taaaggtttc aagaatgcag attttattgc tatatttaaa
      601 aaattattta gtgcggcagg aatgacattg gttctgacga gtgctttttg gtatccgatg
      661 tttgaacaaa tgctctacca aaaaatcaac aaacctagcg tgacaaattt atatgctcat
      721 gcatctaatg tgttcgatag tctgactgaa gctatgaaca acgacttaac tacttactca
      781 atgggactag ttggtctgtt atctttatgt attcccctga ttttatttaa aaaattaaca
      841 aacatagaaa aaaagattta ttatggtacg tgtttgactt ggctagctac aactagtttg
      901 gtgccatggt atcttctgca aaatacgcca gccaaattgt tacaatttcc ttggcggatc
      961 ttaagcttgc aaataatatt ctcttctctg atattaacga tgatattttt c
//
LOCUS       DAZULG010000275         1003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000275 DAZULG010000000
VERSION     DAZULG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.286"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACG9CY_002963"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACG9CY_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAZULG010000276          980 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000276 DAZULG010000000
VERSION     DAZULG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.287"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..940
                     /locus_tag="ACG9CY_002964"
     CDS             287..940
                     /locus_tag="ACG9CY_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4939381.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          396 a           97 c          181 g          306 t
ORIGIN      
        1 gagcgactcc tttggtaggg tgatgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc gccttcgatt ttccaacaac
      121 acaaaatatt atagaaccct ttattctaaa ggatttcaag gtgtctgttt gcatttatta
      181 gaaaaaagaa aaagtattgg caacagaagg tgtcaccaac actagatatt atagagccat
      241 aatatgctgt ataatatcta gtgtataggc ttaagtggag gtatatatga aaatattaac
      301 tgttgaagat gataatatga taagagaggg aataagtgaa tatctttcag aatttggata
      361 tactgttatt caagctaaag atggaagaga agccttgtca aaatttaata gcgatataaa
      421 tttggttatc ttagacattc agataccttt tataaatggt ttagaagtgt tgaaagaaat
      481 tagaaagaaa agcaatttac caattctaat cttgactgca tttagtgatg aagaatataa
      541 aattgatgca tttactaatt tagtagacgg atatgtagaa aagccatttt cattgccggt
      601 cttaaaagct agaatagatt ctttaattaa gaagaattat ggacatttag agaagtttga
      661 atataaggat ctatcggtta attttaatag ctatacagct aaaataaatg gtgaagaaat
      721 agatgtaaat gcaaaagaac tagaagtatt aaaatgttta ttggataata atggacaagt
      781 gttaacaaga atgcaaatta ttgattatgt atggaaagat agcgaagaaa ctccttatga
      841 tcgagtaata gatgtatata taaaagaact tagaaaaaaa ttacaattag actgtataac
      901 tactataaga aatgtaggat ataaattgga gagaaaatga aaaaattaaa gatatttcca
      961 aaaatgttca tacaaatatt
//
LOCUS       DAZULG010000277          940 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000277 DAZULG010000000
VERSION     DAZULG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.288"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG9CY_002965"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG9CY_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4939382.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          277 a          193 c          141 g          329 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa
//
LOCUS       DAZULG010000278          934 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000278 DAZULG010000000
VERSION     DAZULG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.289"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..270)
                     /locus_tag="ACG9CY_002966"
     CDS             complement(<1..270)
                     /locus_tag="ACG9CY_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4939383.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEK"
     gene            complement(270..>934)
                     /locus_tag="ACG9CY_002967"
     CDS             complement(270..>934)
                     /locus_tag="ACG9CY_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4939384.1"
                     /translation="AKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLP
                     DWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSD
                     SFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMT
                     DIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIE
                     VN"
BASE COUNT          311 a          159 c          102 g          362 t
ORIGIN      
        1 tttttctaaa aatatgtaat caactttttc aacaattaca tttagtgttt ccagatgctt
       61 aaaagtcgga tttgtaataa aagctatatc ataatgtgta tccaaatcgt ctctattttg
      121 aatatacgtt atttcttcaa actctttttt tccactgcta ctataagctg ttatatcaaa
      181 attaatagaa ttcttttttc catagcttat tagatttttt agatgtctct ttccaattga
      241 ccctaaccca aaaaaaatta ctttcatcat taatttacct caatgtcgtt aattaagtta
      301 ataatatact tatctttttc aaaagtatat tggggttcca ttttcttttc caaaagcgtg
      361 aaaaattctt taatctcctc taaatatgca tcttcaataa tagtatgtga ataattatta
      421 tcatgcataa cctcatcgta caattgaata tctgtcatac tagaagtttc ttcattccat
      481 gaaaatagag aatccgggtt accttcccat tctattaaaa tcttttcacc aattatagtt
      541 aaatttcttt tagctactct tgaaacaaca tctacactta aagttccaac tataccagtt
      601 tcatgctcaa taataagagt gaaactgtcc gaataatcaa tatcaagatt ggataatttc
      661 tgggtcatta cagtaaacga tttaactttc ccaaatgtat ctataatcca aggtaattca
      721 atagcgaata tttctctaca cccattagtt tttttgtttg aaacaaaaaa attgcgataa
      781 tcttcccaag gatgccagtc gggtaaatac tgtcctacat ggtattgata agaaattttt
      841 ttattatgcg tcactttaga cttaatatat tgaatttctt ttctcttcaa aaatgtagaa
      901 gatagataaa gtaatctatc tttcttttta gcta
//
LOCUS       DAZULG010000279          926 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000279 DAZULG010000000
VERSION     DAZULG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.290"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            430..>926
                     /locus_tag="ACG9CY_002968"
     CDS             430..>926
                     /locus_tag="ACG9CY_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFS4939385.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          357 a          156 c          128 g          285 t
ORIGIN      
        1 ttcattgtat ttctgctttc aaaaagttct aatattgaaa gtataattcc acaaatgcct
       61 ataataagta tgggaataag attagaaaat aacaccccga gccaatctgt gaaaattact
      121 atatttacta atatccctat taacatacct atcatattta ttaaatcaat ttttttcatt
      181 agatatttct cctttattaa atctgaagta cttatattaa aataattcta aactatttac
      241 ctcaaaaagt gcaataaatt atttctgaac aataaaatga tagtcaaaac aaaaaaatat
      301 tgttaatcgt tcagagccat ccgagagtgt aaaatatttt gtgtaaatga aaaaatccat
      361 acaaaaaagg aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag
      421 gacttcccta tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcgat
      481 ttaaatgaat tattccgttc gcacttagaa aaagcgataa acacactcct acggactgaa
      541 ttaacggctt ttttagatta cgaaaaatat gatcgcactg gttttaattc aggtaattcg
      601 agaaacggtt cttactttcg atcaatcaaa accgaatatg gtgaattaac attggaaata
      661 cctagagatc gtaatggtga gtttaaacaa caaactttac cagcctacaa aagaacaaac
      721 gatacattgg aaaccactat tatccattta ttcgaaaaag gtgttacgat gtctgaaatt
      781 gctgatttga tcgaaaaaat gtacggtcat cactatactc cacaaaccat gtccaacatg
      841 actaaagttc tgactgaaga agtaaatgcc tttaaatcca gagccttaaa tgataagtat
      901 gtcgctattt ttatggacgc tactta
//
LOCUS       DAZULG010000280          906 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000280 DAZULG010000000
VERSION     DAZULG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.292"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..720
                     /locus_tag="ACG9CY_002969"
     CDS             <1..720
                     /locus_tag="ACG9CY_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4939386.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
BASE COUNT          339 a          143 c          178 g          246 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatcag gaacttacct
      841 atccatatat caatggtttt taaccaatgc tcaatcttta gtcttactag cgattgtggt
      901 cattgg
//
LOCUS       DAZULG010000281          846 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000281 DAZULG010000000
VERSION     DAZULG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.293"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..237)
                     /locus_tag="ACG9CY_002970"
     CDS             complement(37..237)
                     /locus_tag="ACG9CY_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4939387.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            633..782
                     /locus_tag="ACG9CY_002971"
     CDS             633..782
                     /locus_tag="ACG9CY_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939388.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          272 a          144 c          134 g          296 t
ORIGIN      
        1 ttttctttcg aaaagacggt taaaagtcaa tttttattaa gctttaacaa tgtttgttgc
       61 ttgagggcca cgttgaccat cttcaacatc gtaagacact gcttggcctt cttctaaagt
      121 tttgaagccg tcaccttgga tagctgaaaa atgtgcaaat acatcatttc catcttcacc
      181 agtgataaag ccaaaacctt tgtctgcgtt gaaccatttt actgtaccgt tattcataaa
      241 taaattcctc ctgatacgta tcaatgtacg ttttttcgtt gcaataaata tttgataggc
      301 aaaaggagtt ttatctatgt agatatatcg ctcaaattac gtttcaaaat agattactca
      361 tttaatatat catgcttttt ctaataacac aaggataatg caatgaaata taaaattcat
      421 tgtcttctat tgtcttcgaa tcgaagaaaa agttgaataa ccgcgattga ttcacttttt
      481 gttttcttgt ttatttgctt ttatctgcaa cttttgactt taaaatttct taactatgaa
      541 aacctttcaa ttggtggtat acttataaat ggaatttcgt tatcaacgcg ttcgttgcct
      601 ttcagcaagt atttaaagaa aggagaatgg ctatgacatt ctataacaaa gatatacaat
      661 atcgagtaac cttggataca gatttgaatc tatttgtcgt ttttgacaaa gaaaatacca
      721 atcatgtagc gacaggtgtt acgatcgaac aagcagtgca agaattaaaa aaatctgcat
      781 aaggagtgga aaccctcata tggacgattc tcgttcttct ctttaaacgc atctactggt
      841 agataa
//
LOCUS       DAZULG010000282          842 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000282 DAZULG010000000
VERSION     DAZULG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.294"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            249..395
                     /locus_tag="ACG9CY_002972"
                     /pseudo
     CDS             249..395
                     /locus_tag="ACG9CY_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(483..776)
                     /locus_tag="ACG9CY_002973"
     CDS             complement(483..776)
                     /locus_tag="ACG9CY_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS4939389.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          253 a          149 c          120 g          320 t
ORIGIN      
        1 cgaataaacg gtgttcaatt agctgatctt gtattcactt aaaaaatggt aaagtacgag
       61 ttgctattcc ccattttcaa ttttctaacg ctcaggacaa aatactgttt tcacaacctc
      121 attggaataa aagcatatat attgtttcaa gaacgaaatc ttgttatctt tttaaagggc
      181 atctgtccaa aatctaattt taggaggtac gttttggtat ttattcgtac aaataaaaga
      241 gaattatctt tattcatgta acgcatccaa ttgatttttt catgtacgct cctggaacga
      301 ttttactgac gattgcttat ttaactgcga atcgctcatt agcttttatc atagctatcc
      361 atattttgaa taacgttctt ggctttgtat tatagataca gatgacaaaa aaagttcaag
      421 tatgcggtgt agaaccaata tcgcatactt gaactttttt caatgagtat aaattaaaac
      481 ttctaatttt caattcccat catctgatat gcccgaccgt ctgaacttct tttgaaaaag
      541 ccaaatgtga ttagataacg tctttgttcg acataatcgc cactagaaaa tacgttcagc
      601 agtatcgaat taacctctgt ttctgtataa gtcactccat ggtcaaagcg tttcaagact
      661 tctttcaata ggatgtattt cttttttgtt gaacttggta cttgttttac ttttccatca
      721 ttcatgaatg tgtctaaaac tttcatcttt tcttgctccg tataagtata aaccatcttc
      781 tttctccttt tgattctaag aatactccat ttatgttaga atattctgga ttttttatta
      841 aa
//
LOCUS       DAZULG010000283          821 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000283 DAZULG010000000
VERSION     DAZULG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.295"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>821
                     /locus_tag="ACG9CY_002974"
     CDS             <1..>821
                     /locus_tag="ACG9CY_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939390.1"
                     /translation="YMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHIN
                     RALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVT
                     LITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILL
                     GFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLII
                     KLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS"
BASE COUNT          261 a          109 c          120 g          331 t
ORIGIN      
        1 ggtatatgaa tttgaaaaaa attttttctg taaaaatatt tatagtttat gcatttgtta
       61 gtttattata tttatttcca gcatttaaaa acatggaaat atttggcttt tatgatacgg
      121 catttcacat taatcgtgcg ttaagtttag aatctatttt tagctcccct attaattttg
      181 aaacgtttag atcatatggc atgcaagtaa ataattttta cccatggtta actctatatc
      241 ctttattttt attgatcaag tttactaatt tagcaatagg ctataacctt tttctttata
      301 ttgttacgct cattactcta tttatatgtc attatgtaat gtatgaaatt accaaaaaac
      361 atgttactag ctcctttttc gctattatat atacaacatc ttcttttagg agtgtagaaa
      421 tatttcttcg aggggcaatg ggggaattac tcgcaatgtc aatacttcct cttattttac
      481 tcgggtttat aaagctttac gactcaaaaa aagaatcttg ggttatgttg gcgattagca
      541 tgactttatt aatttacact catgttcttt ctgtagctat gactatggta atgattataa
      601 ttgctttaat tattcaattt tatcggaaaa agaaaataga aatcttctta attattaaat
      661 taataaaagc tgctatagtt actttacttc tttcactagc gtttataggt cctatgattg
      721 agcagacatt gtatcaagat ctgaatagac cttatgtgga tatattacaa aaaagagcaa
      781 tttatctggg taaagagttt ttgattggaa gtatagattc a
//
LOCUS       DAZULG010000284          820 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000284 DAZULG010000000
VERSION     DAZULG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.296"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..438)
                     /locus_tag="ACG9CY_002975"
     CDS             complement(139..438)
                     /locus_tag="ACG9CY_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4939391.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          270 a          139 c          106 g          305 t
ORIGIN      
        1 aggataaaat acaatataaa gaaacatctg tttctttatt tagaataaaa aaatacagac
       61 tctgttcaaa taaatgaacc tagcctgcat tatgagattg actatacttt ttaattcatt
      121 gagatgaata actcgttttt ataaatgccc aaaagtaatc ggtgccgaag ccattcctcg
      181 acctaattct ttttcaaatt ttcctattgt tgatatctcc atataaaacg gtgatactgg
      241 tttagacaat tctcccctga ttgctaacgg acgtaaacta cgttccaaat tatttatgac
      301 tttaggataa tactgacctt tttctaagcc tgtctttgcg tctatcaaaa gtatacgttc
      361 cttttctttg atctctggat ttaaaacgag atcatataat tttagtacca tgttttctat
      421 ttcttcactt ttagacatca cgatttcctc ccaaaaatat atttcttccc actacattct
      481 ataaacaaag tatacgctac atctagattt ttttaaatca ttactggatt gttaaaacct
      541 ttcaatacac tgttatggca acgattttta ttttaaaaac attctaaatt aaatcagtaa
      601 aaatttttat tctaagattt ttattagaat tacgcctaat ttgtgtctct cagggcaatt
      661 ctaagaacgt ttcaagagat agcgacacta tcgttatcga aaaaggcgaa aacctatctc
      721 attttctgct aatgtaagtt ggtttagctt ttgttaatat gactttatga aaatggaggt
      781 aattaaatat gatttttcct aaagttaatg aagcaagctt
//
LOCUS       DAZULG010000285          801 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000285 DAZULG010000000
VERSION     DAZULG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.298"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..569)
                     /locus_tag="ACG9CY_002976"
                     /pseudo
     CDS             complement(1..569)
                     /locus_tag="ACG9CY_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          287 a          121 c          123 g          270 t
ORIGIN      
        1 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
       61 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
      121 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
      181 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
      241 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
      301 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
      361 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
      421 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
      481 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
      541 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
      601 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
      661 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
      721 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
      781 agacagtcca cacagaggag a
//
LOCUS       DAZULG010000286          792 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000286 DAZULG010000000
VERSION     DAZULG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.299"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..717
                     /locus_tag="ACG9CY_002977"
     CDS             1..717
                     /locus_tag="ACG9CY_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4939392.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          200 t
ORIGIN      
        1 ttgaaaaaaa ggattttagt aacaattggt ctagcttttt tgttagctgg ttgtaccaac
       61 gggaatgaat cgaaaaaaga atcaacaaaa gaaacagcga aagcaacagt tgcaactact
      121 acaacaacta gcacagagag caagtcaact gcagcaagta cagaaacagc agatacctct
      181 acttcgtcag agtcttccaa aacgactgaa acaactcaaa catctacttc aagttcttct
      241 gtagaggctt catcttcctc ttcagaaagc agcgaacaag ttgaacaaac actgtggaat
      301 gcagacaaat ccgcacaatt agaggcattt atgaccaaat ggggccaaac aatgggacaa
      361 caatacaaaa gttatacgaa tcaaatgagt gtggacttat atggtcttaa agtccctcaa
      421 gctattttga acggggagtg gaagatggct attggtggag tgcctgtttc tgcagaatgg
      481 tcagaaagtg ggacaggtca agcagactat caaatcacag ccgtctattc cgatgcagag
      541 acagaaccat atctgaaaaa gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa
      601 gtattggtga cacaacaaaa tcaggggaat cccgataatt atctttactt caacgagaca
      661 gcgaataatg aattgaaaaa tggctttaat caaatcgttt atggacaagc aaattaatga
      721 gataaacaag gggttgtgac atgtctttta tcacaacccc ttgctcagtt tatttgttag
      781 tcttttattt tt
//
LOCUS       DAZULG010000287          783 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000287 DAZULG010000000
VERSION     DAZULG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.300"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..>783
                     /locus_tag="ACG9CY_002978"
     CDS             19..>783
                     /locus_tag="ACG9CY_002978"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFS4939393.1"
                     /translation="MEKVDEVFNINELIEYLYQTWKKDTDKQQLFEKYDRYFRQKNVD
                     IQNNSALTYPTYQEIRFRQYVKEIERDFQIEFDYYYLQITSIFLNSWIYQIQLTSDDK
                     LYSFINDINSWCSREVQLVNRFIQIGDIYLTKIQKNVLVILFKLMCSKEFQQKRLALI
                     LAHGDLTATCIASTANRIVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIILL
                     VDMGSLEKIYLNLELDAQKKFGLLNNVSTRMAIDVAN"
BASE COUNT          292 a           99 c          135 g          257 t
ORIGIN      
        1 tatattcaaa tggatgattt ggaaaaagta gacgaagttt ttaatatcaa cgaacttata
       61 gagtatcttt atcaaacgtg gaagaaagac actgataagc agcagctttt tgagaaatat
      121 gatagatatt ttagacaaaa gaatgttgat atccaaaata attcagcttt aacatatccc
      181 acctatcaag agattcgatt tagacagtat gttaaggaaa tagagagaga ttttcaaata
      241 gaatttgact attattatct tcaaataacg tctatctttt tgaattcttg gatttatcaa
      301 atccaattaa catcggacga taaattatat tccttcatca atgatattaa tagttggtgt
      361 agtcgagaag ttcaattagt caatcgtttt attcaaatag gggatattta tttaacaaaa
      421 attcaaaaaa atgttttggt tattttattt aagttaatgt gtagtaaaga atttcagcaa
      481 aaacgtttag cactgatttt ggctcatgga gatctaacgg caacctgtat agcaagtact
      541 gctaatagga tcgttgagag atatatatac gacgccattg atatgccact aatgacttca
      601 agtagtgaga ttgcagacaa aattaataga tacttacaag agtgtaccgg atatgaagaa
      661 attattttac ttgttgatat gggttcactg gagaaaattt atctaaattt ggaactagac
      721 gctcaaaaaa aattcggact actaaacaat gtttcaacaa gaatggctat tgatgttgca
      781 aat
//
LOCUS       DAZULG010000288          777 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000288 DAZULG010000000
VERSION     DAZULG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.301"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>777)
                     /locus_tag="ACG9CY_002979"
     CDS             complement(<1..>777)
                     /locus_tag="ACG9CY_002979"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4939394.1"
                     /translation="PGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYIGVEI
                     AEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKK
                     IEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKDIYAI
                     GDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFNYNFY
                     AAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRIS"
BASE COUNT          254 a          135 c           97 g          291 t
ORIGIN      
        1 gctaattctt aattttacac gactattttt tctcaaaaaa acaggtctaa tgaaatcttc
       61 tatttctata taatctatag actcacttaa taatcgtgct gtttttaaat tcaaacctgc
      121 tgcgtaaaaa ttgtaattaa atatagaaaa gccatttgta aattgggaac cagaaggtgt
      181 aaaatcttga ttgtcaatat gttgagttgc aatataagct gttcttagag cattagaaat
      241 agaaaaatca acactacttc tattaatgga cgtaaaaaaa gttgtagcac aatccccaat
      301 agcatatata tcttttatat ttgtttgctg gtattcatta gttagatatg ctccactaac
      361 atctctcaag agttcctttt tgccaattgg actattagat agaaatccta tagcttgtac
      421 gaccatatcc atagagtatt gggtaccact tgtagtgact attttttcaa tcttttttct
      481 tcctaaatat tttttaggac tttctgatag acaaagatgt attccatttt ttgatagtat
      541 ttcttcaact ttatctgaaa aactacggtc gtaataagtc gacaacactc tatctgcaac
      601 atcgaaaaga tatacttctt tccctctttt tcttatggct tcagcaattt caactccaat
      661 atatcctgct ccaataattc ctactttttt aatagtatta tcatctaaag ctttaaaaat
      721 ctctttagca tttggaaaat tctttatcga aaaaatatta gctaaattat tccccgg
//
LOCUS       DAZULG010000289          770 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000289 DAZULG010000000
VERSION     DAZULG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.302"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..482
                     /gene="ssrA"
                     /locus_tag="ACG9CY_002980"
     tmRNA           116..482
                     /gene="ssrA"
                     /locus_tag="ACG9CY_002980"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          250 a          126 c          150 g          244 t
ORIGIN      
        1 aaaggggcag ttattatata aataatggga tatgattcgc ttttttattg aatttatccc
       61 aatatatgct atactaattt tgtatgaaaa atacacgtaa cccttgttag ctaacgggga
      121 cgttacggat tcgacaggca cagtcgagct tgaattgcgt ttcgtaggtt acgtctacgt
      181 aaaaacgtta cagttaaata taactgctaa aaacgaaaac aactcttacg ctttagctgc
      241 ctaaaaacag ttagcgtaga tcctctcggc atcgcccatg tgctcgagta agggtcctaa
      301 ctttagtggg atacgtttca actttccgtc tgtaagttga aaaagagaac atcagactag
      361 cgatacagaa tgcctgtcac tcggcaagct gtaaagtgaa tccttaaatg agttgactat
      421 gaacgtagat ttttaagtgg cgatgtgttt ggacgcgggt tcgactcccg ccgtctccat
      481 atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt
      541 attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc
      601 aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg
      661 tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa
      721 ggaaaaacac aattatggtt taagcatgtt ccttcaaata agttttttta
//
LOCUS       DAZULG010000290          764 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000290 DAZULG010000000
VERSION     DAZULG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.303"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(237..>764)
                     /locus_tag="ACG9CY_002981"
     CDS             complement(237..>764)
                     /locus_tag="ACG9CY_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939395.1"
                     /translation="GLIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLV
                     ELDVNNYRELYVFNSLDVEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKNIKKCDK
                     CFESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIK
                     KAFKIKTIGTAPNFK"
BASE COUNT          261 a          103 c           85 g          315 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttatattt
      481 ttacctttgt aaaaatattc gattgaatat tctatttggc acgactcact tggcagtata
      541 tgtatgtcaa tatttttcat ctgtttcata ggtatttcaa catctaaact attaaaaaca
      601 tataattccc tgtaattatt tacatctaac tctactaaat ttatctttgg aacaatagat
      661 cctccaccca ttccaccata ataaaaaaaa tcttgttctc tatcaaaata gcacatatta
      721 agctcagttt gtttatcatc ctcaataatt ttataaatca aacc
//
LOCUS       DAZULG010000291          762 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000291 DAZULG010000000
VERSION     DAZULG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.304"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>762)
                     /locus_tag="ACG9CY_002982"
     CDS             complement(<1..>762)
                     /locus_tag="ACG9CY_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4939396.1"
                     /translation="GKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPEL
                     EEWQARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTE
                     SKNRWMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRY
                     LPSKDYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFD
                     YGSAIRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
BASE COUNT          233 a          162 c          143 g          224 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acgtactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac ct
//
LOCUS       DAZULG010000292          742 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000292 DAZULG010000000
VERSION     DAZULG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.306"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACG9CY_002983"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACG9CY_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>742)
                     /locus_tag="ACG9CY_002984"
     CDS             complement(570..>742)
                     /locus_tag="ACG9CY_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4939397.1"
                     /translation="NKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQ
                     QVTDTLVSMFTE"
BASE COUNT          228 a          136 c          116 g          262 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt ga
//
LOCUS       DAZULG010000293          731 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000293 DAZULG010000000
VERSION     DAZULG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.308"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>731
                     /locus_tag="ACG9CY_002985"
     CDS             <1..>731
                     /locus_tag="ACG9CY_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4939398.1"
                     /translation="LCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDW
                     IEAVNNDLNNHLLTLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKY
                     QRKTHTKGWKDTVIQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEV
                     NCPVHADYLNYRINAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDI
                     STTKIYVNTPDVVDKSVFEAFQKGL"
BASE COUNT          277 a          132 c          121 g          201 t
ORIGIN      
        1 actatgttat atcgaattca accgaactac caaaattatt caatgtatta ccgcgcctaa
       61 gaaaatggct ttagaagaaa aaaggataag ggaagggaat caagcattat catcagaaga
      121 gctgactgat tggatagaag cagtaaacaa tgacttaaat aatcacttgc ttacacttca
      181 cgattacaca cttttcatgc ttaccttata tctaggtgat agaaaaagtg agacttatgc
      241 tctgcaatgg aaatatattg attttaataa aaatacggtt cgtctcaaat atgctttaga
      301 taaatatcaa agaaagacac atacaaaagg ttggaaagac accgttattc aggcaccaga
      361 agttgtgatg acattattaa gagaatggaa atcagctcag gctgaacaat tgttgaaact
      421 aaaaattata caaacacctg accagtacct ctttacctat tccaaacctt ctggagaagt
      481 aaattgtcct gttcacgctg actacttaaa ctatcgaatc aatgcaataa aaaaaaggca
      541 tcctgaattg gctcaattat ctccacacaa acttcgtcat acctatgcaa ctatagctag
      601 ggaaggtgga gcaaatatga atcaaatctc taatgcttta acgcactcgg atatttctac
      661 tacaaaaata tacgtgaaca cacctgacgt agttgataaa tctgtttttg aagcatttca
      721 aaaaggattg a
//
LOCUS       DAZULG010000294          724 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000294 DAZULG010000000
VERSION     DAZULG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.310"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..719
                     /locus_tag="ACG9CY_002986"
     CDS             150..719
                     /locus_tag="ACG9CY_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS4939399.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT          231 a           90 c          144 g          259 t
ORIGIN      
        1 gatccggaat ttctttataa aacatgcaat agaattattt atttaaaaaa tggtggaata
       61 aaaaaagata taaaacttag agtagaagat tcaactttga tagaacagat ttttaatgac
      121 ataacgaaat agaattgagg agaatatata tgaaaacaaa agattatatt tttattggtt
      181 tagctacagt tatttcccta gtaatttact ttttttcaat aatggttagt agtattggtg
      241 gggcgtttgg tcacagcata tcccccggga tttttggatt attatctgga atcatatttg
      301 tttatattag ctataactac cctagaaaag gaatttttac tatttacaca attgttttat
      361 tgttattttt tacgttaatg ggcggggcgt acttaccttg gtttatcagt tcgattagta
      421 tggcgatctt agctgatatt attctatctg tatttggata tgatcgagca attcctcaag
      481 ttgcgtcttg ggctctaatg caattaggca gtgctgccgg acagtggatt cctatatggt
      541 tctttacaga tcgttttcgt caagattgga tagataaagg acaatctgct gcaacaatgg
      601 atgcaatgat ccactatgcg gtaggtatat ggggaatcat ttcagtgtta gttgtagcaa
      661 gtttatctat gattggtgta ttgattggaa gaaaagtctt aaaaaaatac aaaaaataga
      721 acgt
//
LOCUS       DAZULG010000295          717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000295 DAZULG010000000
VERSION     DAZULG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.311"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..270
                     /locus_tag="ACG9CY_002987"
     CDS             <1..270
                     /locus_tag="ACG9CY_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939400.1"
                     /translation="FIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLASKKTNSFGL
                     ILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          253 a          111 c          141 g          212 t
ORIGIN      
        1 tttatcgaag aaacacaaaa tatcgagggt tcttatattg aagtaaaaac actctttgtc
       61 aatgaagata aaaccaaagc attggatatt tataaaaaat tagcttcaaa aaaaacgaat
      121 agttttggat tgatattaag cgagtataaa atcaaagcag aagagagtta tttctatcaa
      181 ttactaaaac ggtggagtaa gcttcccgcc gatttttatc gaaaaatgca gatcatcaac
      241 tatcaaccgc ttgctgagac acatgcctaa atagaggttg tgaaatcagt gctttgagct
      301 aataccgaag gtcagctgat tgaacattgt ttagaagaga ttgtgacaac tgcgatttgc
      361 tttgagtaat aagaacgctg gacagaaaat agcttctcat attttctagt ctagtgggct
      421 tattgccgag aagcagcaga tggaacatcg tttagaagag attgtgacaa ctgcgatttg
      481 ttttgagtaa taagaacgct ggacagaaaa tagcttctca tattttctag tctagtggac
      541 ttattgccga gaagcagcag atggaacatc gtttaaagaa gagatcggta caaaataaaa
      601 atatctaaat ttaagtttta tctaagtttc gctttgtacc tttgattcaa atcaaaggag
      661 gcgatcttga tgctgaaaaa atggatcaca ctattttttg ccactcccca ttctaga
//
LOCUS       DAZULG010000296          711 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000296 DAZULG010000000
VERSION     DAZULG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.313"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..>711)
                     /locus_tag="ACG9CY_002988"
     CDS             complement(57..>711)
                     /locus_tag="ACG9CY_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939401.1"
                     /translation="SRSISEYLKGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIAS
                     GNQGNMNITSNKTSRVLLALMGYGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQN
                     LQLENMRLEDLFRLAEDDKYRFEVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIP
                     KRFRGKINAGDQVKVLNRNSTDVVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          216 a          133 c           87 g          275 t
ORIGIN      
        1 tatgcgtttt tttgttgatt taccaacatg tttagacatt tttctctagg aaaaaattaa
       61 ctagcaccac gggttccttt atttttatta acaagtttta tcacaggctt catatatttg
      121 attatatctt catcttctac atagatatct gttatcatta cgacatctgt actatttcga
      181 ttgagtactt tcacttgatc tccagcattg attttccctc taaatctttt aggaatgaac
      241 caactataca tatgattagc atcaccaaaa tgtgctttta tcgcattctc aggatataac
      301 ttattcaata gttcattctt ttgaattttc aaaacttcaa atctatactt atcatcttct
      361 gctaatctaa ataaatcttc taaacgcatg ttttctaact gtaagttttg aaaatcttcc
      421 ggtataaatt ttctatattt cattaataat gaacgcccag cccgatttat tagaatctgg
      481 ccattttggt catctctata aagaattccg taacccatca acgccaatag cacacgtgac
      541 gttttgttac ttgtaatatt catatttcct tgattacctg aggcaatgaa agacttatct
      601 acttgaatta gggtgaaatt ctcattcaaa tatggtagta aaattaatat atgtgaaaat
      661 cgaatcttga gatagctatt ccccttgaga tattcactga tacttctact c
//
LOCUS       DAZULG010000297          705 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000297 DAZULG010000000
VERSION     DAZULG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.314"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>705
                     /locus_tag="ACG9CY_002989"
     CDS             <1..>705
                     /locus_tag="ACG9CY_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4939402.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYE"
BASE COUNT          239 a          135 c          148 g          183 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgag
//
LOCUS       DAZULG010000298          701 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000298 DAZULG010000000
VERSION     DAZULG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.315"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..358)
                     /locus_tag="ACG9CY_002990"
                     /pseudo
     CDS             complement(245..358)
                     /locus_tag="ACG9CY_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          258 a          105 c          120 g          218 t
ORIGIN      
        1 aaagagtata cgaacaaacg ttctttttgt aaaggtggaa tttattatac taaataaata
       61 ggtgaacaag tacttgtgcc aaattatgtg ccaaaaaaaa tcgaatttaa taaaacacaa
      121 acaaaaagaa atcctattat gataacattt cttataataa caaacacaac agaagacgtg
      181 taatagttag tcaggaacgt acaaataacc cctgtatcct ttgcggtaca gggggttatt
      241 ttgtataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa
      301 gtatatttac tccttgataa agtaaaatag atgcatgcgt atgtcttagt ccatcaataa
      361 acagtcgaag agaaaatagg gaaaaattga ttactaaaga aaaacttact aggtcgacgg
      421 tgtagttgat tttcatactt caatagattc gaattaaacg atttcgattg gcttatacca
      481 gtcctatcca acgaaagata tatcgttctt aactctagcg gtataacttg aagctttacg
      541 taaagccaat gggaatgaac tggagatgac tttttattat ttttagtaat agctgatggg
      601 ttaatactta caactttcct tttcaaagta ccatataaaa aaacggtggg gttgtttagc
      661 gaaataaagg atctaagcca atagcttaga tcctttattc t
//
LOCUS       DAZULG010000299          683 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000299 DAZULG010000000
VERSION     DAZULG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.316"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..124
                     /locus_tag="ACG9CY_002991"
     CDS             <1..124
                     /locus_tag="ACG9CY_002991"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4939403.1"
                     /translation="RMIWNETGRPGYGLYDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(232..>683)
                     /locus_tag="ACG9CY_002992"
     CDS             complement(232..>683)
                     /locus_tag="ACG9CY_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS4939404.1"
                     /translation="QTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKK
                     QPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSH
                     IKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT          217 a           98 c          122 g          246 t
ORIGIN      
        1 aagaatgatc tggaatgaga caggaagacc aggatacggc ttatatgatc gagcattagg
       61 tgctgtttat ctaaatggat tatgggaagc aatcgaaaaa ggaaacaaaa gtaaagataa
      121 ttagtgaaaa aaactgtaat tttattatgt aaataagaag gatgttcaaa ttcttgatac
      181 ataaatccgg aggttgttaa taagcattcg gatttatgta tcttatttgg tctaggaagt
      241 aacttgattt tcttttttcg attgatccat gagtgcttct tcttttaatt caaagatata
      301 ttctgagatc ctcataatca cttttttcca gtcttctggt gtgatgattc cttttaaaat
      361 atagattttt ttgatatgag aaggcaattg tttcaaaggg cagtcgctga taacgatgtt
      421 gcagttctcc ataaaatcat tttgtatttt gatcgattca tttgaaaaaa tttgttctat
      481 cttaacgatc agatctttac tgatataaaa atcacgcgta tagttcacac caatcacaat
      541 tcgcggctgt tttttgtagc gatcatacaa atgtactagg tcttcaacca tccattgcac
      601 attttccttt tctaaaataa ctggtaacaa gtgttgattt tgttctctga aatccatata
      661 aaattgttca atggcggttt gga
//
LOCUS       DAZULG010000300          628 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000300 DAZULG010000000
VERSION     DAZULG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.318"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            378..608
                     /locus_tag="ACG9CY_002993"
     CDS             378..608
                     /locus_tag="ACG9CY_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939405.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          224 a          103 c          102 g          199 t
ORIGIN      
        1 aacagcggaa gcagaagcaa ctccttcgga aataagctga aattcacaaa aatttgaaaa
       61 gcaatttccg tgaatttctt cttatttctt ggagttaaac gcttctgttc cagcctcttt
      121 ttatctgtct ttcactaatg atcagttgta gataagcaag ttgctttttc ttacatcata
      181 ccgcccatca agacaaacgt ttttagcttg tacgtactgg acaaaaatcc cattatatca
      241 agaaaaaaca acgttgataa tacttaaaat tattaggcta gaacggacta gtaagagttt
      301 tttgcccctt ttttgcccct caaatatcgc tttacagcca gaacataagt tcgtataatc
      361 gttttgaggt gatttttatg atggaagact ttattagaaa aaatatcagt gatgaatatg
      421 cagactttta tgagcaaagc agcaaaaaag ataaattcca gatggatgtt tcaattttag
      481 ctatattagc tttttccgaa aataaacaac ctgtaactgc aaaaaaagaa acagtattct
      541 ctgaaggcaa aataaaaact cgatatatat tagaggtaga aactaatttt aaaaacagat
      601 cggagtaatg gttatgcttt ttaatgag
//
LOCUS       DAZULG010000301          601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000301 DAZULG010000000
VERSION     DAZULG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.321"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(191..264)
                     /locus_tag="ACG9CY_002994"
     tRNA            complement(191..264)
                     /locus_tag="ACG9CY_002994"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Ile,seq:cat)
     gene            complement(453..>601)
                     /locus_tag="ACG9CY_002995"
     CDS             complement(453..>601)
                     /locus_tag="ACG9CY_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4939406.1"
                     /translation="YHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
BASE COUNT          198 a          111 c          108 g          184 t
ORIGIN      
        1 ctttctattt aaaaatcaaa gtaaaacttt tcaaatatac agaaatataa ctatgtgaaa
       61 catcctttca ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga
      121 agaaaagctt tagcttgtat aatactcttc aaaaaattct aacacagaac gattcaaatg
      181 gctacgttaa tgtaccctgt aggactcgaa cctacgaccg gacggttatg agccgtctgc
      241 tctgaccaac tgagctaagg gtactggttg ttgccacata aagccataaa caatcaacca
      301 gtagaatgtg tggcaacaaa cctgttatcg catagcttgg agtgtgacta ttatgggtga
      361 tagtgaagat atgcgataac atcactattt tatcgaatga tttttatagt tgtcaatata
      421 gttatgtaat gctcctcaac gaggagctat ttttatcgtt taggaatatt taaataccaa
      481 cgtttgtcat ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc attcgttgcc
      541 cgcatcatga catagacttt cttattaggg aagttacgca tgttaaaaga tacatgatag
      601 c
//
LOCUS       DAZULG010000302          587 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000302 DAZULG010000000
VERSION     DAZULG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.322"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..414
                     /locus_tag="ACG9CY_002996"
     CDS             <1..414
                     /locus_tag="ACG9CY_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS4939407.1"
                     /translation="SLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTED
                     AIKYAIVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIA
                     VRTGWISKPGQTSICLPHKLVISIEKKESTDYYIY"
BASE COUNT          209 a           77 c          123 g          178 t
ORIGIN      
        1 tcgttaatga aatttttaag aaaagctggt gtcaagatag gcgatgtcat aattattggc
       61 attttgatta ttacctcttt tattccctta tttgtattct ctatagaaaa ggagaatgaa
      121 acaactgaag atgctatcaa atatgccatc gttcgaattg atggcaagga aatggatcgc
      181 ttcgatttgg ataagatcaa tcataaactc gtgaattatc atcctgcgaa gggacaatat
      241 aatattgttg agattaaaga tggaagaatt cgagttaaag aagataatag tcctgatcaa
      301 atcgcggtta gaactggatg gatctcaaaa cccggtcaaa cgagtatttg tttaccccat
      361 aagttagtta ttagtattga gaaaaaagaa agcacagatt actacattta ctaatctgga
      421 aatagatgag gaggttttta ataagaaaaa gatattatta ttaataatag caggggcttt
      481 attaagtgtg ttaagcgctt gtggacaaga aaaagaagcg gtgacaacag gcacaagtat
      541 ctttaacggg acctgcaatg gtttagttaa cagtcggtta gattact
//
LOCUS       DAZULG010000303          584 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000303 DAZULG010000000
VERSION     DAZULG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.323"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..>584)
                     /locus_tag="ACG9CY_002997"
     CDS             complement(39..>584)
                     /locus_tag="ACG9CY_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4939408.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          167 a          110 c          101 g          206 t
ORIGIN      
        1 gaaatccact ccttttttca tgctctaaaa tgatattttt acacaactgt tacggttgtt
       61 ttacgaaatt ggacgggact ttgccagttt aatttctgtt ttatccgttc attattgtag
      121 taatagatat agctatcgat ggctgatttt aattcctcga aacagttata aactttcccg
      181 tgaaaaatct cttgttttaa taagccaaaa aaattctcca taagtgaatt atctaagcag
      241 ttccctttcc gagacatgct ttgaaagatt ttgtactcct tcaatttgtt tctataagct
      301 ttcatttgat acgcccaacc ttgatctgag tgaaacgtac gacgataggg acaatcgtta
      361 gtggtctgga tagcttcctc caaaccttcc attaccgcta atgcattggg cttttcagag
      421 atacgatagg ataaaatctc actattgaac atatccataa aaggatctag atacaatttc
      481 ttttgacgga taatgccttt ggcatccact tcatggtatt taaattcagt tgtatccgtc
      541 gtgattttct gatggcagat actggtgtag aaacgacgat ggat
//
LOCUS       DAZULG010000304          576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000304 DAZULG010000000
VERSION     DAZULG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.325"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>576
                     /locus_tag="ACG9CY_002998"
     CDS             <1..>576
                     /locus_tag="ACG9CY_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4939409.1"
                     /translation="KTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKD
                     INFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDY
                     QFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASL
                     SKTNLFLFDEPTSGLDYINMERIADLIKDLK"
BASE COUNT          231 a           65 c          109 g          171 t
ORIGIN      
        1 aaaaactgaa aacaataacg cattaaggaa atttgacatt tttaatagtt cgattgaagt
       61 tccctatatc gaaaaggtaa gtgcagatgt attaaaagtt gaagaaatta cttatgaaaa
      121 tattctaaaa gacataaatt ttgatgtcaa agttggagat gttattggtt tggtagggaa
      181 aaacggtgtg gggaaaacaa ccttactaag aatactcatg aagattattc gccctaccaa
      241 aggaaagata atcgaaaata aaaaaaattt gtcatcgccg tttttagtaa tgcaagaaat
      301 ggattaccaa tttttcacag aatcagtgag gagcgagtta gccctgggga atgaaatagt
      361 tagtaaagat cagcaagaaa aaattttaaa aaaaatggaa ctttataaaa tgaaagatca
      421 attaccgttt gatttatccg gtggagaaaa acaaagatta ttggtttcta tagcttcatt
      481 gtcaaaaact aatttatttc tatttgatga acctacaagt gggttggatt atattaatat
      541 ggaaaggatt gctgacctta ttaaagactt aaaaga
//
LOCUS       DAZULG010000305          575 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000305 DAZULG010000000
VERSION     DAZULG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.326"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..575
                     /locus_tag="ACG9CY_002999"
                     /pseudo
     CDS             258..575
                     /locus_tag="ACG9CY_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          195 a          105 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DAZULG010000306          572 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000306 DAZULG010000000
VERSION     DAZULG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.327"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..221
                     /locus_tag="ACG9CY_003000"
     CDS             <1..221
                     /locus_tag="ACG9CY_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4939410.1"
                     /translation="KFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKY
                     FFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            222..>572
                     /locus_tag="ACG9CY_003001"
     CDS             222..>572
                     /locus_tag="ACG9CY_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4939411.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPL"
BASE COUNT          221 a           63 c          107 g          181 t
ORIGIN      
        1 gaaaatttca attgtttact ttagatgaat gtatcaatgt tgacttatta aataataaaa
       61 taagttttga agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat atgtatgaga
      121 aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg aatgatttat
      181 atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt ttatttacta
      241 tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat ttggcaggaa
      301 agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa catcctgaac
      361 ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg acgactaagt
      421 taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat actgtaggga
      481 aaattgaagt gatacgtgat tcattcttac aactagaaag agaagataat atatttgatt
      541 taattataga tttagatttg acttcacctt ta
//
LOCUS       DAZULG010000307          569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000307 DAZULG010000000
VERSION     DAZULG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.328"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>569
                     /locus_tag="ACG9CY_003002"
     rRNA            <1..>569
                     /locus_tag="ACG9CY_003002"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          139 a          131 c          173 g          126 t
ORIGIN      
        1 gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccagtggc
       61 gaaggcggct ctctggtctg taactgacgc tgaggctcga aagcgtgggg agcaaacagg
      121 attagatacc ctggtagtcc acgccgtaaa cgatgagtgc taagtgttgg agggtttccg
      181 cccttcagtg ctgcagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt
      241 gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa
      301 gcaacgcgaa gaaccttacc aggtcttgac atcctttgac cactctagag atagagcttc
      361 cccttcgggg gcaaagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt
      421 tgggttaagt cccgcaacga gcgcaaccct tattgttagt tgccatcatt cagttgggca
      481 ctctagcaag actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg
      541 ccccttatga cctgggctac acacgtgct
//
LOCUS       DAZULG010000308          567 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000308 DAZULG010000000
VERSION     DAZULG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.329"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..125)
                     /locus_tag="ACG9CY_003003"
     CDS             complement(<1..125)
                     /locus_tag="ACG9CY_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFS4939412.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="ACG9CY_003004"
     CDS             complement(143..463)
                     /locus_tag="ACG9CY_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4939413.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT          185 a          111 c           68 g          203 t
ORIGIN      
        1 ccgtatagat tttcattcaa aaaaatgatt ctgggttttg gatagtagaa accaggctct
       61 ttcatctcca tataaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaaa ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt
      241 ctgataaatc cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag
      301 agacatcgaa atagtcagca atccgactta attcatctga attaggagta ttatttttcc
      361 actttgctag ataaccgtta gaatatccaa aattaatttc taactgccgt atagatatct
      421 ttttcctctt cgccaagtct tttattattt cataggtatt catcgataaa acaacctttc
      481 taaatgttta caaaaaaagt ttagaaaaat acgcagaaat aatttgacta attctgagta
      541 atgagctata ctatatcttg taaacag
//
LOCUS       DAZULG010000309          559 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000309 DAZULG010000000
VERSION     DAZULG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.330"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..509)
                     /locus_tag="ACG9CY_003005"
     CDS             complement(144..509)
                     /locus_tag="ACG9CY_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939414.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          220 a           73 c          108 g          158 t
ORIGIN      
        1 ttattacaca actgttttta gtgtttggag agaagaaaag aagtaaaaaa tagaaaaact
       61 agtgatagtt ttggtgatat aagaaaaaca tatatgtata aaaagaagaa agtagcttct
      121 ctttgtacat atatggtttg tttttatgga tgccgagctt ttttataaga taaggctacc
      181 gcccataacg acccaaggat ttgtggaact aagtaaatac ctgcaagaag caagatcagc
      241 tgggtcggtt ttaaaggatg atagaagtaa cgccattcag taaaattggc gcaaaataaa
      301 taggaagtga cagtagatat aatattggca atgaataaag atataatacg cctgttcctt
      361 tgaaaataat atccagcaaa aagagataaa aatatcaatg tgatgaaccc gcttacggaa
      421 tgataacgaa aatcaagcag taaggcgaaa tgcacataag gaacaaaaga taaaagcaac
      481 ggaaggtttt tccagcaagt ttttatcatc tttcatcacc acctaacaag tgttattatt
      541 ttgttagttt tattgtaaa
//
LOCUS       DAZULG010000310          538 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000310 DAZULG010000000
VERSION     DAZULG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.333"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..370)
                     /locus_tag="ACG9CY_003006"
     CDS             complement(<1..370)
                     /locus_tag="ACG9CY_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS4939415.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHI"
     gene            complement(372..>538)
                     /locus_tag="ACG9CY_003007"
     CDS             complement(372..>538)
                     /locus_tag="ACG9CY_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4939416.1"
                     /translation="VLIVHHDLSKVKKYFDNIILLNRNLIAHGSVESVFNEENLKKAY
                     GDTIFIGEGE"
BASE COUNT          216 a          100 c           59 g          163 t
ORIGIN      
        1 aaatatgaaa taaatccgtt gagctatttg caactcctat aaggataact cctagtgcta
       61 aaaaagaact gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta
      121 taaaagtaat aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa
      181 tacctaaaat ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca
      241 taagagacat tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa
      301 ctacagatgt aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat
      361 tttgaagcat attattcccc ctctccaata aatatagtat ctccataggc tttttttaaa
      421 ttttcttcat taaatacaga ttctacactt ccgtgagcaa ttagatttcg attcaaaaga
      481 ataatattat caaaatactt ctttaccttg cttaaatcat gatgcacaat tagcactg
//
LOCUS       DAZULG010000311          537 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000311 DAZULG010000000
VERSION     DAZULG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.334"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..465)
                     /locus_tag="ACG9CY_003008"
     CDS             complement(<1..465)
                     /locus_tag="ACG9CY_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4939417.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFIN"
BASE COUNT          193 a          100 c           76 g          168 t
ORIGIN      
        1 gttaatgaaa aagcctaagg caactgataa aacaggtgtt actatggctt gagcactatc
       61 tggaatcaag tgagcaattg ccatatgtgt tttagccgcg ttgtcatcaa ttgtctttcg
      121 aatatatccc gaagaattta aatcaaaaaa acgaaaactt gcttctgtta aaccgctaat
      181 tccctttttt ctcaaattag tctctaagcg aaacccaagg atatgagaaa ataagccaga
      241 aaaaatataa aataaagctc ctaaagttaa ccaagcaaca gtttgaaccg cataactctt
      301 agctaaatat tcatctccta caataattag agactgaaga aatttgtaaa tactgtaata
      361 tccatagact gttaatacga cagaaaaatc agaaaataaa gtggctaacc atccgtagta
      421 tttcatttta gggacataac taaataattt tttgtatact ttcatttttt cctcccccgc
      481 attcaatttc ttctcttatt cgtttacaac tgttaatgct ttccgtaata gcgtaaa
//
LOCUS       DAZULG010000312          519 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000312 DAZULG010000000
VERSION     DAZULG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.335"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..>519)
                     /locus_tag="ACG9CY_003009"
     CDS             complement(67..>519)
                     /locus_tag="ACG9CY_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4939418.1"
                     /translation="VQDIDKWITIGVSVLVLVIVVLFFKELLITSFDPLMAKAIGMNV
                     SFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGAS
                     SSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERSL"
BASE COUNT          191 a          103 c           63 g          162 t
ORIGIN      
        1 atgccccgaa tataaaaaga gcaatcaaaa tccccagact tcccagtact gctttccatt
       61 ttctcatcat aacgatctct ccttctttct taaaaatttt tgttttggtg agattagaaa
      121 actaattaca aataagaatg ccgctgttaa aacaatactt gatcctgctg ctatgttaaa
      181 actataacca ataaacaatc caagcaatga tgaacttgct ccgactgttg cagaaataaa
      241 tatcatagtt ttcaagctat ttgcatataa atatgctgta gctgctggtg taattaacat
      301 tgcaacaatt aatatagttc ccacactttg catagctgtt actgctacta aagttaatag
      361 aaccatcaat agataatgat aaaaactgac attcatccct attgcttttg ccattaatgg
      421 atcaaaagaa gtgattaata attctttaaa aaatagaact actataacca acactaaaac
      481 cgaaacgcca attgtaatcc acttatcaat atcttgtac
//
LOCUS       DAZULG010000313          514 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000313 DAZULG010000000
VERSION     DAZULG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.336"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG9CY_003010"
     CDS             complement(<1..132)
                     /locus_tag="ACG9CY_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4939419.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZULG010000314          478 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000314 DAZULG010000000
VERSION     DAZULG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.340"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(312..>478)
                     /locus_tag="ACG9CY_003011"
     CDS             complement(312..>478)
                     /locus_tag="ACG9CY_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4939420.1"
                     /translation="SNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKY
                     TFVDPERYKD"
BASE COUNT          165 a           78 c           71 g          164 t
ORIGIN      
        1 attgtgagat aataataaag aagagtttaa agcgttcccc aaaaaccact gccccataag
       61 tgtgttacgc tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga
      121 aaaaaagtga gaaaaaaggc ttataattgg agtggtagtt aattagtgac ttatttttga
      181 ttttatagca ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa
      241 cttaaaaaat atctcctttc ataagtatgg tgataaaatc cgttccgggc taccttttta
      301 ggtagcctac tttaatcttt gtatctttct ggatcaacga aagtatactt tatatagtca
      361 taacgccgat gatcgcttcg agcgtctagc acatcagtca cgatatcaaa caaaaaatat
      421 acgtctttct tcattctagt tttcgcagca gggattttaa agtagttctt attagaat
//
LOCUS       DAZULG010000315          468 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000315 DAZULG010000000
VERSION     DAZULG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.341"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACG9CY_003012"
     CDS             <1..>468
                     /locus_tag="ACG9CY_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFS4939421.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZULG010000316          362 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000316 DAZULG010000000
VERSION     DAZULG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.347"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>362
                     /locus_tag="ACG9CY_003013"
     CDS             <1..>362
                     /locus_tag="ACG9CY_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFS4939422.1"
                     /translation="VIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIV
                     MVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVA
                     SAIVNILLNMIFIPRLGI"
BASE COUNT          117 a           42 c           69 g          134 t
ORIGIN      
        1 cgtaatatgg atgtctacta ataacgcctg ggttccatgg ttctatggtg agatgagtaa
       61 aaataatgaa aaaaaagtga agaaagttag taactattat ttagtagtct ttactttgat
      121 tacgatgatt gtaatggtag tttcgattga tttagcaaaa tttatggctc cgaaagacta
      181 ttattctggg ttatctttag tggttccgat aaccttaggc tatttctttc agtttttata
      241 tagtttacca gttaatgctg aattttatga gaaaaaaaca tcgtttattg cgattggtac
      301 agtagcaagt gctatagtta atatactatt aaacatgatt ttcataccta ggttaggaat
      361 aa
//
LOCUS       DAZULG010000317          336 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000317 DAZULG010000000
VERSION     DAZULG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.351"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..327
                     /locus_tag="ACG9CY_003014"
     CDS             121..327
                     /locus_tag="ACG9CY_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS4939423.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          120 a           43 c           81 g           92 t
ORIGIN      
        1 ataaaattga acaactggac gggcaaattg atgccttgct ttcagaaaga gaaatattga
       61 tagaagaaaa caagcgattg aaaaagaaag tagcccaacc aatgaaggga ggtggatagt
      121 atgaacaata gaacctttga tattttgaaa tggatcgcta tagtagttat ccccgcttta
      181 gcgacttttg tcggtcttgt cggaaaaggt gttaactggc ggtacacaga tataacggtg
      241 atgattatta caggttttgg agcatttcta gggagtattt taggtgtatc aaatcgaacg
      301 tataagaaaa tttcatcaga taaataggag gataga
//
LOCUS       DAZULG010000318          321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000318 DAZULG010000000
VERSION     DAZULG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.354"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG9CY_003015"
     CDS             complement(<1..>321)
                     /locus_tag="ACG9CY_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4939424.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZULG010000319          302 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000319 DAZULG010000000
VERSION     DAZULG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.355"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>302
                     /locus_tag="ACG9CY_003016"
     CDS             <1..>302
                     /locus_tag="ACG9CY_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS4939425.1"
                     /translation="NLPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHV
                     LYSREMVESLCTVFDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQ"
BASE COUNT          100 a           60 c           44 g           98 t
ORIGIN      
        1 taatctgcca atttgtcgcg aacaacgcat cttagtgaat attttagctc gactttctat
       61 cacgaaaatc gaatcggaag aaacaagctt ggagcaatac aaacttttaa aaaaagcaaa
      121 cgttcctcat gttctttatt caagagaaat ggtggagtcc ctttgtactg tatttgattt
      181 gtttatacct gaaaaagaaa agattcttta ctcattggca ttcgcacgaa ataaacttta
      241 taatggattt cttaacttca atcaaccaaa tacgcccctt cctactttca tgctttatca
      301 ag
//
LOCUS       DAZULG010000320          302 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000320 DAZULG010000000
VERSION     DAZULG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.356"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>302
                     /locus_tag="ACG9CY_003017"
     CDS             <1..>302
                     /locus_tag="ACG9CY_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFS4939426.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          117 a           48 c           68 g           69 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 ag
//
LOCUS       DAZULG010000321          289 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000321 DAZULG010000000
VERSION     DAZULG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.361"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>289
                     /locus_tag="ACG9CY_003018"
     CDS             <1..>289
                     /locus_tag="ACG9CY_003018"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4939427.1"
                     /translation="KEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNL
                     AEYGIEVICYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARI"
BASE COUNT           94 a           54 c           61 g           80 t
ORIGIN      
        1 aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt caatgtgcat
       61 gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta taagcagaca
      121 atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat gcctgtattt
      181 gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac gttagctttt
      241 atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcg
//
LOCUS       DAZULG010000322          281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000322 DAZULG010000000
VERSION     DAZULG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.denovo.363"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>281
                     /locus_tag="ACG9CY_003019"
     CDS             <1..>281
                     /locus_tag="ACG9CY_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFS4939428.1"
                     /translation="VGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEYGKLLYQI
                     WKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDT"
BASE COUNT          100 a           43 c           54 g           84 t
ORIGIN      
        1 gtgggctact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat cttatatgat
       61 cgtggcatta acgtttctca tacgacgatt tatcgttggg tgcaagaata tggcaaacta
      121 ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt attcatggaa aatggatgaa
      181 acgtacatca aaattaaagg aaaatggcat tatttgtatc gagccatcga tgcagatggt
      241 ttaaccttgg atatttggtt acgtaaaaaa cgggacacac a
//
LOCUS       DAZULG010000323        21896 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.guided.044, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000323 DAZULG010000000
VERSION     DAZULG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.guided.044"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1693
                     /locus_tag="ACG9CY_003020"
     CDS             <1..1693
                     /locus_tag="ACG9CY_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4939429.1"
                     /translation="PDLQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAK
                     TVLHFDKQDKGPPSLKPNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQ
                     AGKAIRSTIRHHRLKPYWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFW
                     QKQQIKRQYAKAARTAGQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKG
                     ALAVLVLGLLMLLIIGGLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAEL
                     QDYLDNYEATHDYDEYHFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQ
                     YILTENVVVETRYRTETDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIM
                     DEETLSFYSLYMSTLGNRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEA
                     EKYIGYPYVWGGSSPSTSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKP
                     GDLVFFKGTYDTPGVSHVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            1716..1967
                     /locus_tag="ACG9CY_003021"
     CDS             1716..1967
                     /locus_tag="ACG9CY_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFS4939430.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            1957..3186
                     /locus_tag="ACG9CY_003022"
     CDS             1957..3186
                     /locus_tag="ACG9CY_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFS4939431.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            3183..5264
                     /locus_tag="ACG9CY_003023"
     CDS             3183..5264
                     /locus_tag="ACG9CY_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS4939432.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            5413..9333
                     /locus_tag="ACG9CY_003024"
     CDS             5413..9333
                     /locus_tag="ACG9CY_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFS4939433.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            9334..10278
                     /locus_tag="ACG9CY_003025"
     CDS             9334..10278
                     /locus_tag="ACG9CY_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939434.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            10282..10485
                     /locus_tag="ACG9CY_003026"
     CDS             10282..10485
                     /locus_tag="ACG9CY_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFS4939435.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(10785..11021)
                     /locus_tag="ACG9CY_003027"
     CDS             complement(10785..11021)
                     /locus_tag="ACG9CY_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4939436.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(11204..11575)
                     /locus_tag="ACG9CY_003028"
     CDS             complement(11204..11575)
                     /locus_tag="ACG9CY_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS4939437.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(11572..12900)
                     /locus_tag="ACG9CY_003029"
     CDS             complement(11572..12900)
                     /locus_tag="ACG9CY_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4939438.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(12861..13190)
                     /gene="mobC"
                     /locus_tag="ACG9CY_003030"
     CDS             complement(12861..13190)
                     /gene="mobC"
                     /locus_tag="ACG9CY_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4939439.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(13448..13819)
                     /locus_tag="ACG9CY_003031"
     CDS             complement(13448..13819)
                     /locus_tag="ACG9CY_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4939440.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            13964..14149
                     /locus_tag="ACG9CY_003032"
     CDS             13964..14149
                     /locus_tag="ACG9CY_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4939441.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            14307..14474
                     /locus_tag="ACG9CY_003033"
     CDS             14307..14474
                     /locus_tag="ACG9CY_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939442.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            15092..15751
                     /gene="vanR-B"
                     /locus_tag="ACG9CY_003034"
     CDS             15092..15751
                     /gene="vanR-B"
                     /locus_tag="ACG9CY_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFS4939443.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            15754..17097
                     /gene="vanS-B"
                     /locus_tag="ACG9CY_003035"
     CDS             15754..17097
                     /gene="vanS-B"
                     /locus_tag="ACG9CY_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS4939444.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            17268..18074
                     /gene="vanY"
                     /locus_tag="ACG9CY_003036"
     CDS             17268..18074
                     /gene="vanY"
                     /locus_tag="ACG9CY_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS4939445.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            18092..18919
                     /gene="vanW-B"
                     /locus_tag="ACG9CY_003037"
     CDS             18092..18919
                     /gene="vanW-B"
                     /locus_tag="ACG9CY_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS4939446.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            18916..19887
                     /locus_tag="ACG9CY_003038"
     CDS             18916..19887
                     /locus_tag="ACG9CY_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS4939447.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            19880..20908
                     /gene="vanB"
                     /locus_tag="ACG9CY_003039"
     CDS             19880..20908
                     /gene="vanB"
                     /locus_tag="ACG9CY_003039"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS4939448.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            20914..21522
                     /locus_tag="ACG9CY_003040"
     CDS             20914..21522
                     /locus_tag="ACG9CY_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS4939449.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(21588..21761)
                     /locus_tag="ACG9CY_003041"
     CDS             complement(21588..21761)
                     /locus_tag="ACG9CY_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939450.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         5504 a         5584 c         5976 g         4832 t
ORIGIN      
        1 cccggattta cagccctata tccgaaaatc ggacaaacgg gccgataagc tggataaggc
       61 ccgcgccgcc atccccaaga agcgcgtccc ggtcaaggaa accgtctttg acgaggcaag
      121 cggcaaagca aagaccgtcc tgcactttga caaacaggac aaggggcctc cctccctgaa
      181 acccaatccg gcaggccgtc ctgtttccga ggtttttcta ttcggccacg ggaaaatcca
      241 tgaagtcgag catgaaaacg tgggtgtgga gggcggccat aagggggagg aacttgccga
      301 gcggcaggcc ggaaaagcca tccgcagcac catccggcac cacaggctga aaccctactg
      361 ggacgcggaa aaggccgagc gcaggctatt gtctgccaac gcggagtatt tctaccagaa
      421 atctttgcgg gataacccgg agatcgcaca ggcggccagc aaccccattt cccggttctg
      481 gcagaaacag cagatcaagc gccagtacgc caaggccgcc cggacagcgg gccagaccgc
      541 aggcgcggcg gcacagggcg cggccaagac agccggggcc tccacgaaag cggccaaggc
      601 cgcagccaaa gaaagtgaac gggcggcctc ctttgtggcc cgccactgga aaggggcgct
      661 ggccgtcctt gtgttggggc ttttgatgct gcttatcatc ggcggcttac agtcctgcac
      721 cgccatgttc ggcagcgccg gaagcggcat cacggcgtcc tcttactttt ccgaggatag
      781 cgatatgttg gccgccgagg acgcctacgc ggagctggaa gccgagctgc aagactatct
      841 ggacaactac gaggccaccc atgactatga cgaataccat tttgaccttg acgagatcag
      901 ccatgacccc tatgtgctga tttccattct gtcggcgctc catgacggga tatttaccat
      961 cggggaggta cagggtgatt tgtccatgct ctttgacaag caatatatcc tgacagaaaa
     1021 cgtcgtggta gaaacccgtt accgcaccga aaccgataca tggacggacg cggagggcaa
     1081 cacccataca gacacctacc aagtgccgta tgactattac atctgctacg tcacgctgga
     1141 aaacttcgac ctgtcccatg tgccagtcta tatcatggac gaggaaacgc taagtttcta
     1201 ttccctgtat atgtccacgc taggaaaccg gcctgatctg ttttccggca gccagtaccc
     1261 caacgcctcc accctgacgc cgccgaccta ctacgacgta cccccggagg ctttggagga
     1321 tgccaccttt gccgccatgc tcaaagaggc ggaaaaatat atcggttatc cctatgtgtg
     1381 gggcggcagc tctcccagca ccagctttga ttgcagcggc ttcatttcgt gggtaatcaa
     1441 tcatagtgga tggaacgtgg gacggctggg ggcgcagggc ctatataaca tctgcacccc
     1501 tgtttccagt tcgcaggcca agcccggcga ccttgtattt ttcaagggaa cctacgacac
     1561 ccccggcgtg tcccacgtcg gcctgtatgt gggcaacaat gtgatgctgc attgcgggaa
     1621 cccgatttcc tacacgaacc ttacttcaaa ctactggcaa tcccattttt acagttacgg
     1681 gcgtttacct taacgccaga aaggagcttt caattatggc aaccaaactt gaccgcatcg
     1741 aaaaggacat ccagaaaacc aagtctaaga tcgcggagtt tcagaaacag cttcgggagc
     1801 tggaaacgca gaaaaccgaa caggagaatt tgcagatcat ccagcttgtg cgcggcatga
     1861 acatgaagcc ggaggaattt gccgcgttct tacgcagcgg cgcaatgcag gccgcccctg
     1921 ccgcaacccc ttaccatgaa caggaggaca atgcccatga agaataagac cagacggatt
     1981 tcccgtattc tccccgcact tctggccgtg atgctctgcg tcacggcctt tcccgttacc
     2041 gcccttgcag gcggcaatga cccggcaccg gccccgctgc cggaggctac aaccgaaccc
     2101 gctacgggcg gtttggagcc tgaaacagac gaaaccggca ccgccacgga cgggacagaa
     2161 accgaggccg aaaaagatac cggccctctg accggcaagg acatcaccga cctgttttcc
     2221 gcgctgttcg gttccaaggt gagcattgcc gccacggacg acggcatcca gatcaccacc
     2281 ggcaccgaca aaaaagaacc tgaacagacc ggcaccgtca ccaccaacgg cggcaggctg
     2341 aatgtccgca ccggcgcggg gcttgacaag accgcgttca cccagcttcc caatgggacg
     2401 acggttgagg tagtcggcac agacggcgac tggattaaaa ttctcttgcc ggagcgcatc
     2461 ggctatgtcc attccgacta tatgaccgtc agcgaaaagg aagtagcggc taccggcgag
     2521 ggcggttttt ccctctccat tgacccggag gaaattgctt ctctgctgga actgttcaat
     2581 ggcggcgggg ccagcgccgc cctgtccccg gacgggaacc tgtcactgat tgacgacatc
     2641 ggcagcgccg cgaaaaatgg caagcagttt atcaccgtgg aaaccaaaaa cggcaatgtg
     2701 ttttatctga ttattgaccg ggacgacgag ggaaaagaaa ctgtccattt cttaaatcag
     2761 gtggatgaag ccgatctgct gacgctgatg gaggacgggg aaaccgtcac ccccgctgct
     2821 gtctgctcct gtaccacgaa atgcaaggcc ggggctgtga acacgaattg cccggtatgt
     2881 aaaaccaacc tgaccgagtg cagcggcccg gagccgcagg aaccccagcc ggaggaaccc
     2941 gaagccccgg aggaagaacc caagggcggc gcgggcggcc tgatcgtgtt cctgctggtg
     3001 gcgctggcgg gcggcggtgc ggccctttac tatttcaagt tcaaaaagcc caaggctgaa
     3061 accaccggca gcgacgattt gggcgagtat gattttgggg aggacgagga tttggaggac
     3121 gaggaacccg aaaccgagtt tgacccggaa gctgattttg tgagtgaaca ggaggacgag
     3181 gaatgacata taaacttgta atcgcggaaa aacccagcgt ggcccgcagc attgcgggcg
     3241 tgatcggggc cacggagaaa cacgacggct atttggaggg gaacggctat ctggtgagct
     3301 ggtgcatcgg acatctggtg tcctttgccg acgccggacg ctatgacgag cgtttcaaaa
     3361 aatggcgcta tgaggatttg cccattctcc cggaaacatg gcagtacatc gtccccgacg
     3421 acaagaaaca gcagtttgac gttctctgtt cgctgatgca gcgccccgac gtgaccggcc
     3481 ttgtgtgcgc caccgacgcc gggcgcgagg gggaattgat tttccgtttc gtctaccaga
     3541 tggcgggatg ccaaaagccc tttgagcgcc tttggatttc ctctatggag gacagtgcta
     3601 ttaaggacgg ctttgcccac ctgaaaccgg gcggcgacta tgatccgctg tatcaatcgg
     3661 ccctgtgccg ggccaaggcc gactggctga tcggcattaa cgccacccgg cttttttccg
     3721 tcctgtacca caagaacctg actgtgggcc gcgtccagac gccgacctta aaaatgctgg
     3781 tggacaggga cgccaagatt accgatttca agaaagagaa atatcacatc gtccatatca
     3841 cggcgggcgg cgcggacgcg gtaagcagcc gcttttccga cgctgccgag gcaaacaccg
     3901 tcaaggcggc ttgtgcaaaa gcgcaggccg tttgcgtttc cgtgacgcgg gagaaaaaga
     3961 cggaacagcc gcccaagctc tatgacctta ccaccttgca gcgggaggct aaccggcttt
     4021 tcggctatac cgccaagcag acccttgact atgcccaggc actgtatcta aagaagctgc
     4081 tgacctatcc gagaacggat agtcaatatc ttaccgacga tatgctgccc gccgtggaaa
     4141 gccttgtgtc cggcctgtgg gagctggtgc cgtttgccaa gggcctgaac ctttccccgc
     4201 agtttgaccg catcctgaac agcaaaaagg tgtccgacca ccacgccatt atccccaccg
     4261 ctgaatttgt gaagcagggc tttggcactc tggccgagag tgaaaaaaag ctgctgtccc
     4321 tgatctgctg caagctctta tgtgccgtgg ccgggccgca tatctatgag gccgtcaccg
     4381 ccacatttac ctgtgccggg caggagttta ccgccaaggg caagaccgtc ctttcccccg
     4441 gctggaaaga gattgaacgc cggttccgtt cttccttaaa gaccgacgcc gacgaggacg
     4501 ccgaggcggt gcgggagctg ccggagcttt gcgagggcca gacctttgca gacgtggccg
     4561 cctctgtcac cgagcatttc accacgccgc ccaagcccta caccgaagat acgcttttgt
     4621 ccgctatgga acgggccggg gccgaggata tgccggagga tgccgagcgc aagggattag
     4681 gcacaccggc cacccgcgcc gccattctgg aaaagctggt gcagatgggc tttgtccaga
     4741 gaaaaggcaa gcagctcatt cccaccaagg acggcatcaa tctggctgtg gtgctgccgg
     4801 aggcgcttac ttctccccag cttactgccg aatgggaaaa ccggctgacc gagatcgcca
     4861 aggggcaggc cgatcctgac gaatttatgg acggcatcga gacgcaggcg cgggagctgg
     4921 tgcagaccta ttcctgtatc agtgaggaaa agcagaaatt gtttcaggcc gagcgtgttc
     4981 ctatcgggac gtgtccccgc tgtggtgagg ctgtctatga gggcaagaaa aactactact
     5041 gcggaaaccg ggcctgccag ttcgtcatgt ggaagaatga ccgatttttt gaggaacgca
     5101 agaaagcgtt caccccgaag atcgccgctg cgctgctcaa aaccggcaaa gtcaaggtaa
     5161 agggcctcta ttccgtaaag accggcaaaa cctacgacgg gacggtgctt ttggctgata
     5221 ccgggggcaa gtacgtcaac taccgcattg aacacaggag ctaaaggaca gtaacacagc
     5281 aagcataggg agtgtggcta cactcccggt aaaatccccc ggccgcgtcc ctgccgggcc
     5341 ggggattttt gcttgttggg gaagtcccca accccctatt ttttgaaaca gaacagagaa
     5401 aggagtgacc gcgtgaacga agtattttct atccaagtcc ataaccggca gtttttggaa
     5461 cagggcaaac aaggcgtctg gctggatttg cccaccacaa ccgaaacgct gcaagccgcc
     5521 ctgcgggatg ttggcattac tgccgacaat ccgcaggatt tttttatcaa cgggtattcc
     5581 tgcccggagg acagacacct tgccattccc tatgacatgg tgctggccgc cgacgtggac
     5641 gagctgaatt tccttgccgc ccggctgggc cagcttgacg ccgccgagat cgcagagctg
     5701 aacgccgccc tgcaaaatcc ccgtggcggc tttgagaata tcggccagat catcgactac
     5761 accgaaaatg tggattacta tgtgcatctt cccgacgtta ggggggatgg acagttgggc
     5821 gactactacc tgaaccgttc cggcatggtg gatatgccgg aggaatggaa agccgggatt
     5881 gatacggcgc agtttggacg ccacattgcc cagcaggaac aaggcgtttt tacccagtac
     5941 ggctatctgg tgagaagcgg cgacgaatgg cagcgcgtcc atgagggcca gccggtgccg
     6001 gaggaatacc gggtgatgca ctacccccag ccggaagctg accgggagga accgcaggcc
     6061 cagcagccgg aggcatccga aagccccgac aaagcgccgc ccccggttat cccgatcatg
     6121 ctggacggca tgaacagcaa agaacgcatg aaagtgatta ctgaccgtct ggaaaccggc
     6181 gtacaggagc tttttgaaag tagccgctat atggcttact tatccgctat gtccaagatg
     6241 cacgattaca gtctgaacaa tacgctgctg atcgccatgc agggcggttc gattgtggcg
     6301 ggctataaca ggtggcagga tgaatttgac cgccatgtga agcggggcga gaaaggcatt
     6361 aagatactgg ccccggcccc gttcaaggtt aagcagcaga tggagaaaat cgacccggcc
     6421 acccagcagc ccgtgatcgg cgcggacggc aagcccgtga ccgaagaaca ggaagtgacg
     6481 gttccggcct ttaaggtggt gacagtcttt gacatcagcc agaccgaggg caaggatttt
     6541 cccatgccct atatcagtga acttaccggc gacgtgcagc agtatcagga tttctttgcc
     6601 gcgctggaac ggacatcacc ctatcccata agcattgagc cgatttcaga cggggccaag
     6661 ggccgctgct tctacgcaaa acagcgcatt gccatcaacg agggcatgag cgaattgcag
     6721 accctaaaga ccgccatcca tgagattgcc catgccaagc tccacgccat tgaccgggag
     6781 gcccccgccg aggaacaggc ggcccggcca gaccgcagca cccgcgaggt acaggccgaa
     6841 agcgtggcct ataccgtctg ccagtattac gggctggaca cgtcggacta ttcttttggc
     6901 tatgtggccg attggagcag cggcagggag ttatccgagc tgaaagcctc ccttgaaacc
     6961 atccgggcca cggccagcga attgataacc gagatcgacg gccattttgc ccagcttcag
     7021 caggagcgcg aggcccggca ggaacaggag gccgtcacgg ttgacgttac cccggaggca
     7081 gcggccccgg cgcaggccga ggaagaacag gccccggccc ttgatccggc agccgagccg
     7141 cttgtgactg tgctatggag cgaaagcccc cagctaaagg acggccagca aatgccgttg
     7201 catgaggccg acgcgctgtt tgcccgtctg gatgcagcat atccggcaga ggcaggctat
     7261 gacaaaaccg cgttccgcat tgactgtatc tttcagggcg agccggacag ctacgagggg
     7321 cggcaggatt ttggagatag ggacggttcc ctgatcgaac atatccagag ctaccaccaa
     7381 ttttacgcgc aggacgaaaa ctggaaaaag catgtaatcg ctacaaccgg catagagggc
     7441 tgggagcagg acaaggccca tcgggatatg ctgttaggcg agtttgtccc ctatatgaag
     7501 ctgcattgca gccttgccca aatggaacag acggcgcagg gccgcttaca gtccggggaa
     7561 accctgacgc cggaggaaac ggcctatttg ggggcgcttc tctcttatgt gcaggagtgc
     7621 cgccccatgc tcaatcaggg ccagtatcag ttgccggagc cgccgaaact ggctgacttc
     7681 gaccagagct tgcaggacta caaagcacag gtacaggccg agatcgcgca ggaggccgcc
     7741 gcagccggta tgaccgtgga ggaatacgcg gcggctggct atgaagcacc ccaacaggac
     7801 accttttcca tctaccagct tcgaggcggg gaggaaaccc gcgactatcg gtttgagcca
     7861 tacgagcgtt tacaggccgc agggctggcc gtggacagtg ccaactatga ccttgtttat
     7921 acggcaccgc tggataaaag cacgtctttg gaggatattt accgcacatt caacatcgac
     7981 cacccggcag attttagggg acacagcctt tccgtttccg acgtggtggt gctgcaccag
     8041 aatggacagg acaccgccca ctatgtagac agcttcggct atatggaagt gccggagttt
     8101 ttgcgggaaa atcccctgcg taccgccgaa ctgtctacgg agcagaacga aaacatgatt
     8161 gacggcatta tcaacaatac gccgcccgaa ccgcagccgg aggaacccga acagctatct
     8221 tttgaggatg tggcagttgc ttccggccag acacaggagg aacccccgga ggctgcgccc
     8281 ccgcaaacgg ctgtccgcta ttatcccatc aatgagggag ccgcccgccg cgccaaggaa
     8341 gccatgagct tttctgacta ccggcccggc agcgccacgg cggaataccg gcactatgtc
     8401 gatcaggccg tggaaattgc ggagcgccag aaaaagcgtg tggagccaga gtatcacgaa
     8461 aagatagaca gcctccttga cacctacgcc cgcaagctgg ccgagaacat gaataagggc
     8521 tatgagatta ccgcccgcgt cccctctgtc ctgattgccg ggccgtccaa tttcccggta
     8581 cgggcgaaag aaaagcagaa cgccgccagt gaccgcaaca tggaggaatt tcagtacatt
     8641 cagggcttgc ttgacaaaat ccgcagcacc ggcatgggtg gtatcagcgc cgacgatccg
     8701 caggccgttt ccaagctgga aaagaaactg gaaaagctgg aagcgtcgca ggagttgatg
     8761 aaggccgtca atgcctacta ccgcaagcat gggacgctgg acggatgccc ccatctgacc
     8821 gaaagaggca ttgaaaacct gaaagcggat atggcgtcgg gctggcacta tgagaaaaaa
     8881 ccgttccagt cctggcagct ctccaacaat aacgccgaga tacgccggtt aaagggccgc
     8941 attgaggaat tgacccgcca aaaggaggcg gcctatgtgg gatgggagtt tgacggcggc
     9001 actgtggaga tcaaccgggt ggccaaccgc ctgcaaatct tctttgaggg gaagccggat
     9061 gctgctgtac gcgacgagct gaaaagcaac ggtttccgct ggtcgcccaa ggccggggca
     9121 tggcagcggc agttaaacga caccacaatc cgcgtcgctg accgtatcaa gtgcatccag
     9181 cccctttccg gcgaaaagcc cagcgcctta cagcttgcgg cccgcaggga gaaagaaaag
     9241 ccctctatcc gggcgcagct caacgcggcc aagaccgaac aggccaagca gccaccggcg
     9301 cgggaaaaat caaagggatt ggaggtagag taaatgcaga agtttgaaaa tgtggacgtt
     9361 ctggccgcgc tggaacagat catgcggcag aacaccgcgt tttatcaaag tgactttggc
     9421 attgacaaag acattatcag gcgggatgcc gccagcgatc aggccgcaga caagaccctg
     9481 ctctggatgt cccggccctc cgggacgtac tgtttccggg aacgtgatgt tttcctgaaa
     9541 gacacccgac agtacaacac atggaaattt tacggggagc agacccgtga caagatactg
     9601 gcctacgcgg tggagctgac cggcacacag ggcggcacta tccggggcaa tctctatgag
     9661 ctggactatc cgcagcattt ccggcacgtc attgaaaccg cccagccggt gagtgtgaac
     9721 cggctctttt atgagcatgg cacccgtgac atcccggaga gccagcgttt tgacggaagc
     9781 cctgaccggg tattgggaaa ctttttgtac tatgaggccc agccccatga cccggctgtg
     9841 ctgcaggagg cgctacggac ggagcagcac agccgggggc gggccgtccc cggcgatttc
     9901 aaagtccacg tcaccgcttt gcatgacagc cggattgaaa ccgaggcccg gcgtattgtg
     9961 gacagcctga aagatctgtc cgcgcctaac agccccaata aaacgcattt catggtggag
    10021 atctccccgc actttgcggc gattgcttcc accaaggata atgaccggct ctttgccatg
    10081 ctgccctata aatccctgtg ttttaccggg atgaaaggca ggcatggcgt gtatgccgtg
    10141 attaaccggg acgagcgccg ggatgtgagt attcgcaggc cccggccctc tatccgcaag
    10201 cagctatccg acagcaaaca ggccgccagc ccaaaaaagg cggcggccag aaccaaaaag
    10261 aatgaattgg aggtataagg aatgatgcac tttaccgtgg aggaagaaaa cctgatctgt
    10321 atgtaccaca atgcagaccg gcgcagaacg ataaacaggc tcaccgccgt catgctggtt
    10381 atggatgggg atatgcgggc gcaggcccag cagacccttt ccaagctgga acgcatgacg
    10441 gatgccgact atgacggcca gaagttccac ttcacagacg aataacgaac agcagacgcc
    10501 gggcgcggcg gccatgtggt cgcttgcgtc cggcgtcttt tctttttgcc tattcctacg
    10561 catttagaac ccggtattgt cggggatagg atgaagtatt cgcttgcccc tgcaaacatg
    10621 cgggagaagc cttgcagcgg gcggttttca atcccttaaa tgcgtaggtt gcagctataa
    10681 ttagacccct gaaacagata tagccgcgca gcgggtgtat ctgtttcagg ggtgtgcagg
    10741 tgatagccgc aaagcggcgt aagagggtgc agccctcttg ccgtctatct ctgttccagc
    10801 gggaagctcg gcaatgcttc cagcagtttc agggaaatgc gctgccgtat atcttcgtca
    10861 atgactttct tctttttgcc gtttgcctgt ctgatctcca ccgtggaaag ctcgttgatg
    10921 tagtcagcat aatgggccaa cactttttcc gtggcccacc tctcaccggc aacggcggct
    10981 ttgatcgttt catagtccag cagcttttct tgtccgtcca tcctgatacc cccgtcagac
    11041 tgattttttc tatgatacca tattcggaag catggaacca gagaaaacgg cgcttttctg
    11101 ctggtaaatt ctacgcattt agaacagcac ttcggcgggg ataggataaa acacccacat
    11161 accccgctga ccatgccgga aaagccttga aatataaggg cttttacagc tctaagtgcg
    11221 taggtttcca gtatcttttc cgctggcgct cggcctcttt gcggcggcgc atccgtttgg
    11281 agcaggccgc gcagtatttg gcccggttag acttcggcgt gaacgtggcc ccgcactcac
    11341 agcagcgttt cacttcgtcc cggtctttgg tgatctcggc atacagggcc gcatccagcg
    11401 ggaggacagc ggccttaaac cagcggcaca gaagcgaata ggaaatgctc tgtacgcaaa
    11461 tacattcttc gccgtcgtcc agcaacaggc agttgccgtc acaatagttg cagcactcat
    11521 gcgtcagctt ccgggccttg cgatattgag ggtaattcat tcgtggaata ctcatagctc
    11581 ttgcgtccat tcctttcccg gcgtcatacg caaaatgccg tccacattct gcttgatgat
    11641 gccgtattcc tgcatctgct ttttcgcttc gccgtacttc tcatacagcc gttccttttc
    11701 ctcggccaat cgcttgtact cggctttcag ggcgtacaga tcggggagct ttttcacacc
    11761 ctgtcccttt agggcctttg ccgcagcttc atacaaaatc aacgtgcttt catgctcacg
    11821 ccggaatttt gccttatccg ctgcattgcg gtattccata gccacgggcc agagctgctt
    11881 gtaggtagaa atattcttta tcagcaccgc catatccgca agccggtgtt ccatagcttt
    11941 gagggtagcg gcggcctcgt cgctggcggc ggtgatctcg gccactttgc tttccaaatc
    12001 cgggtaggtg tctatcccat gttcagtcag gaaattcaaa gtctttgcgg cctgcttcaa
    12061 attgtggagc ttcgcccacc tctcatagcc tgccgactgc tgggccttaa tgttgttttc
    12121 cagatcaatc cgcaaagaaa tccccttgcg ctctttgggc gctctggtga taacgcggcc
    12181 tttgatacgc tctttgatgg cttcctcggt gtaggcttcc cccaatgtct tgcagcgggt
    12241 aaagcgttcc tgtccgggag cgcggaaaga aataaacttt cccggcttga tctcatagcc
    12301 ttgcgcctgc atgagccgca ggaaatcatc aaaatctttt gcctgcggga tagcggcgtc
    12361 aatggctgct ttgagcttcg ctttccagct cgtccctttc cgctgggcgt cccactcggc
    12421 atagctcttg cccttactct ggccgggctt caccacggac aatccgtttt ccttgcacag
    12481 cctgtcgctg gtgcgccgga tataggcata gctctgtttg ttggaaatgt acttgcgctg
    12541 atttaccata tccacggcac agaaaataat gtgattgtgg acgtggccct tatcaatatg
    12601 ggtagtcagc acatagggat atttcccttg cagcacttcc tcggcaagct gcttcccgat
    12661 ctcatgggcc tgttccggcg tggcttcgcc gggggcaaag gattgtatca ggtggtggcc
    12721 cagattattg cccttttggt atgcctgcgc cagcagcatt tcaaattcaa tgtccgctgt
    12781 ttcataagag caggcaaagg aggccaccag cagcttgccg tcagttttgt ccgggttctc
    12841 gatgtagtca agggcttttt tcagcgttga cttgatagga tgtatctttg taactgccat
    12901 atctgcgcca gcgccccctt tatatcctcg atgtcctgtg cgtacaccgg ccccataccg
    12961 ttcagcctct ttgcaatctg attgacgttg acgccgatct tctgtatctc ggcggtctgc
    13021 gcccggatgt cgctggtgtc catgttgacg atatagccgt caatgagcat cttccgggcg
    13081 taggctgaaa agttcttcgt atgcagcagc gccattttct gttcaatcat agcccgttcc
    13141 tccggcgtca cagggacgcg cagcacgatt tttcgctttc tgtttgccat gcttcaccgt
    13201 cctttccatt caggggtttg gggatttccc caacaagcaa aacggacgcg gcccggcagg
    13261 gacggggcag gccgtttttc cgggagtggc tacactccct atgcttgcta cgttactgtt
    13321 tctccactaa caagacgttt ccaaagcccc aaactacccg caaaatagcc ctctggaaag
    13381 aaaaaagtgc aaaaaaatag aagctacgct gtgcagctcc cgctaaaagt aaaacagagg
    13441 atgccagtta cacatcctcc gtttcattct tcttcggctt ttcaattagt atggcgctgt
    13501 caatcgcgcc ctcgatgatc ggcaggtatt cttcggggca gagtttcagc ttgtggctga
    13561 ccctttgccg cagttcgctt tcctcccgca tgatctccgg gttaaagtag cgttccacag
    13621 gaagcccgca ggctttaata agctgtatga caaccggcag actgggaatg gccccgtcgt
    13681 tttcgatgtt ggcaagatag cgccagtcaa tatttatttt ctctgccatt gctttgcgcg
    13741 aaacgcgctt tgcttgccgg gccgctttca catccgcgcc gaaagtttca aagccgggac
    13801 aatcttcaat attcgccata tcgcacctcc cacttacatt gtatatttca tactctgccc
    13861 gtggaatgtt ggtatatgca cctttcttaa agtttatatt tcacattttg tttcttttat
    13921 tcttcgagca aaagaattat aatgtaccct gtggaggtgt actatggact acatgacgtt
    13981 gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg ataaactact attgtgctgc
    14041 gggacgcatc cccggcgctg taaagatggc gacgatctgg ctcattccca aaactgcgga
    14101 aaagccgatt gacaggcgta caaagaaagg aaaggcggtt actgaatagt ataatgaaac
    14161 cggcgcattt tggatatggt tttgaagaga gagaaagcat ggtattttgt cacggaaaaa
    14221 ctataagatg atagatggca ggctgctcca gaccgataaa aagtattctc atctgaaaca
    14281 gaaagaaaag atttacacat ggatgtatga agaagcgaaa ctttatcatg acagaaatgg
    14341 aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc agccgtatat ggtagaattg
    14401 aaaaggccgg catctggatt ctttatggtg aagtctccag acattggata actgctggtc
    14461 taatgcgcct ttgagtatgg ggtgattgat tttaataccg gggaggtacg gctttttgag
    14521 gaaaatattc tgggatttac atttggcgat tattacattt cctgtaactg caaggacacg
    14581 gatgatgaag atatgaggaa tatagacttg ctgattttct actgtcttga acggtattag
    14641 gactgaatct gctgcgcgga gtgcggcaag agcgaccgga gggagtacac acaggccctg
    14701 cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa agaggccgcg cattttgctg
    14761 aggcttgggg tgtccccaac aagcaaaacg agaacgccgc aggcgggcaa gtggtggatc
    14821 agaatatccg taaaggatat tctgatccat tgcttgccag tattatcggg atgttttgac
    14881 aaccgctgta atcgaaacat cccaaacatt aagaaggtgt taattattat tatcttgatt
    14941 taattcgttt aaaccaataa tatagataca tacaccatga aaagaagcga gtatcccaat
    15001 gacggatgat taaaatccga aaagaaatcg ctttgaaact acagggaaac tacagactgt
    15061 tatgttatct tcttaaaatg gagggatttt tatgtcgata cgaattctac ttgtcgagga
    15121 tgatgatcat atctgtaata cagtaagggc gtttctggct gaggcaggat atcaggtgga
    15181 tgcctgcaca gatggaaatg aggcatacac caagttttac gaaaacactt atcaactggt
    15241 tattcttgat attatgctgc ccggtatgaa cgggcatgaa cttttgcgtg aatttcgtgc
    15301 gaaaaatgat actcccattc tgatgatgac agccctgtcg gatgacgaaa accaaatccg
    15361 ggcgtttgat gcagaggcag acgactatgt aacaaagcca ttcaagatgc agattttact
    15421 aaagcgggtg gaagccctgc tgcggcgcag cggtgcgctg gcaaaggaaa tccgtgtcgg
    15481 caggctgaca cttctgccgg aggatttcac ggtactttgt gacggtacag agctgcccct
    15541 gacacgaaaa gaatttgaaa tccttttgct gctggtgcag aacaaaggca gaaccttaac
    15601 gcatgaaatc attttgtcac gtatatgggg atatgacttt gagggtgatg gcagcacagt
    15661 ccacactcat atcaaaaatt tgcgggccaa gctgccggaa aatatcatca aaaccattcg
    15721 cggtgtagga taccgattgg aggaatcata ataatggaaa gaaaagggat tttcattaag
    15781 gttttttcct atacgatcat tgccctgcta ctgcttgtcg gtgtaacagc aacactgttt
    15841 gcacagcaat ttgtgtctta tttcagagtg atggaattac agcaaacagt aaaatcctat
    15901 cagccattgg tggaactgat tcagaatagc gataggcttg atattcaaga ggtggcaggg
    15961 ctgtttcact acaataacca atcctttgag ttttatattg aagataaaga gggaagcgta
    16021 ctctatgcca caccaaatgc caatacatca aatagtttta gacccgactt tctttatgtg
    16081 gtacatagag atgataatat ttcgattgtt gctcaaagca aggcaggtgt gggattgctt
    16141 tatcaagggc tgacaattcg gggaattgtt atgattgcga taatggttgt attcagcctt
    16201 ttatgcgcgt atatctttgc gcggcaaatg acaacgccga tcaaagcctt agcggacagt
    16261 gcgaataaaa tggcaaacct gaaagatgta ccgccgccgc tggagcgaaa ggatgagctt
    16321 ggcgcactgg ctcatgatat gcattccatg tatgtcaggc tgaaagaaac catcgcaagg
    16381 ctggaggatg aaatcgcaag ggaacatgag ttggaggaaa cacagcgata tttctttgcg
    16441 gcagcttctc atgagctaaa aacgcccatc gcggctacaa gcgttctgtt ggagggaatg
    16501 cttgaaaata tcggtgacta caaagatcat tctaagtatc tgcgcgaatg catcaaaatg
    16561 atggataggc agggcaaaat catttccgaa atactggagc ttgtcagcct gaatgatggg
    16621 agaatcgtac ccatagctga accgttggac atagggcgca cggttgccga gttgctgccc
    16681 gattttcaaa ccttggcaga ggcaaacaac cagcggttcg tcacagatat tccagccggg
    16741 caaattgtcc tgtccgatcc gaggctgctc caaaaggcac tatccaatgt catattgaat
    16801 gcagttcaga acacgccgca gggaggcgag gtacggatat ggagtgagcc tggtgctgaa
    16861 aaatgccgcc tttttgtttt gaacatgggc gttcacattg atgatactgc gcttccaagg
    16921 ctgttcaccc cattctatcg cattgatcag gcgcaaagca gaaaaagtgg gcgaagcggt
    16981 ttaggacttg ccatcgtaca aaaaacgctg gatgccatga gcctccagta tgcgctggaa
    17041 aacacctcgg atggcgtttt gttctggctg gatttaccgc tcacatcaac attgtaaata
    17101 tttaaaactt aaatgatttt gaccgacagg tataaccctg ccggtctttt tttgctgcta
    17161 caggaaaact acagactggc tacagggaaa gtacagatgc gcttgccata ataacaatcg
    17221 taccagccac aaatcgtagt tttattgcaa aggaggcatt cgatcaaatg gaaaaaagca
    17281 actatcattc caatgcggat catcacaaac ggcatatgaa acaatccgtg gaaaagcggg
    17341 cttttctatg tgcgttcatt atttcgttca cagtctgcac gctgtttttg gggcggagac
    17401 tggcttccgt attggaggca acacagatac cgcccatccc tgcaactcat acaggcagca
    17461 gcactgacgt agtggagaat ttggaggaaa acgctcttgc caccgccaaa gaacagggag
    17521 atgaacagga atggagcctg attttagtga acaggcagaa ccccatcccc gcacagtacg
    17581 atgtggagct tgagcaacta tcaaatggtg agcggataga tattcggatt tctccctatc
    17641 ttcaagattt gtttgatgcc gcaagaactg atggagttta cccgattgtc gcatccggat
    17701 accgaacaac agaaaaacag caagaaatta tggatgaaaa aattgccgaa tataaggcga
    17761 aaggctacac ctctgcacag gctaaagcgg aagcagaaac ttgggtggcc gtgccgggaa
    17821 cgagcgagca tcagcttggt cttgctgtgg atatcaatgc ggacggaatt cattcaacag
    17881 gcaacgaggt ttatagatgg ctggatgaaa acagctatcg ttttggtttt attcgtcgct
    17941 acccaccaga caagacagag ataaccggtg tgagcaacga gccgtggcat taccgatatg
    18001 ttggcatcga agctgccaca gagatgtaca accaaggggt ttgccttgag gaatatttaa
    18061 aaccagaaaa atgagaaaag gatgttatgt tatggacaga aaaagattga cacagcgttt
    18121 cccgttcctg cttccaatga gacgggcgca gagaaaaatg tgcttttatg cgggaatgag
    18181 atttgacggc tgtcgctatg cgcagacgat aggggaaaaa tcgctttccc atttgctctt
    18241 tgaaacggat tgcgcattat acaaccacaa taccggattt gacatgatat accaggaaaa
    18301 caaggtgttc aacttaaagc tggcagcaaa gaccttaaac ggcttattga taagaccggg
    18361 ggaacccttt tctttctggt ggctggtacg ccatgcggac aaagataccc cctataaaga
    18421 cggccttacg gtgaccaatg gtaaactcac caccatgtcg ggcggcggta tgtgccagat
    18481 gagcaattta ctattttgga tgttcctgca tacgccattg acaattatcc agcgcagagg
    18541 tcacgaagta aaggagtttc cagagccaaa cagtgacgag attaaagggg tggatgcaac
    18601 catttcagag ggctggattg atttaaaagt gcgaaacgat accgactgca cctaccaaat
    18661 atgggtgacc ttagatgatg agaaaatcat cggtcaggtg tccgccgaca aagagcccca
    18721 agcattatac aaaattacaa atggcagtat ccagtatgtc cgtgaaagtg gcgggattta
    18781 tgaatatgcc caggttaaac ggatgcaagt tgccttaggt accggggaaa taatagattg
    18841 caagctgctt tatacaaaca aatgcaaaat ctgttatccc ctcccggaaa gtgtggatat
    18901 tcaggaggag aaccaatgag aaaaagtgtg ggcattactg tttttggatg cgaacaggat
    18961 gaggcaaatg ctttccgcgc tttatcgccg gattttcata ttatccctac gctgattagc
    19021 gatgcgatat cggcagacaa cgcaaaattg gccgctggca atcaatgcgt tagcgtaggc
    19081 cataagtccg aggtttccga ggcgacaatt cttgcgctga gaaaggtcgg ggtaaaatac
    19141 atttctaccc gcagcatcgg ctgcgatcac attgatacga ctgccgccga gagaatggga
    19201 atctcggttg gcacggttgc gtattcgccg gacagcgttg cggattatgc tttgatgctg
    19261 atgctgatgg ccatacgggg tgcaaaaccc accatgcacg ccgtggcgca acaagatttc
    19321 agattggatc gtatccgggg gaaagaactg ggggatatga ctgtgggagt tattggaacc
    19381 ggccatatcg ggcaagcggt cgtcaaaagg ctgcggggat ttggatgcca tgtgctggcc
    19441 tatgataaca gccgaaaaat ggatgcagat tatgtccagc ttgatgagct tctaaaaaac
    19501 agcgatattg ttacgctcca tgtgccgctt tgtgcggata cccgccatct gatcggtcag
    19561 aagcaaattg gagagatgaa gcaaggcgca tttttaatca acaccgggcg cggagcgctt
    19621 gtcgataccg gggcgctggt ggaggcgttg gaaagcggaa agctgggtgg tgcggcactg
    19681 gatgtgttgg agggcgagga tcagtttgtt tataccgatt gctcgcagaa agtgcttgac
    19741 catccctttt tgtcgcagct tttaaggatg ccgaatgtga tcatcacacc ccatacggcg
    19801 tactacactg agcgtgtgct gcaggatact acagaaaaaa caatcaggaa ttgtctcaat
    19861 tttgaaagga gtttacagca tgaatagaat aaaagtcgca atcatcttcg gcggttgctc
    19921 ggaggaacat gatgtgtcgg taaaatccgc aatagaaatt gctgcgaaca ttgatacgga
    19981 aaaattcgat ccgcactaca tcggaattac aaaaaacggt gtatggaagc tatgcaagaa
    20041 gccatgtacg gaatgggaag ccgacagtct ccccgccata ctctccccgg ataggaaaac
    20101 gcatgggctg cttgtcatga aagaaagcga atacgaaaca cggcgtattg atgtggcttt
    20161 cccggttttg catggcaaat gcggggagga tggtgcgata caggggctgt ttgtattgtc
    20221 tggtatcccc tatgtgggct gtgatattca aagctccgca gcttgcatgg acaaatcact
    20281 ggcctacatt cttacaaaaa atgcgggcat cgccgttccc gaatttcaaa tgattgataa
    20341 aggtgacaag ccggaggcgg gtgcgcttac ctaccctgtc tttgtgaagc cggcacggtc
    20401 aggttcgtcc tttggcgtaa ccaaagtaaa cggtacggaa gaacttaacg ctgcgataga
    20461 agcggcagga caatatgatg gaaaaatctt aattgagcaa gcgatttcgg gctgtgaggt
    20521 cgggtgtgcg gtcatgggga acgaggatga tttgattgtc ggcgaagtgg atcaaatccg
    20581 gctgagccac ggtaccttcc gcatccatca ggaaaacgag ccggaaaaag gctcagaaaa
    20641 tgcgatgatt acagttcccg cagacattcc ggtcgaggaa cgaaatcggg tgcaggaaac
    20701 ggcaaagaaa gtatatcggg tgcttggatg cagagggctt gcccgtgttg atcttttttt
    20761 gcaggaggat ggcggcatcg ttctaaatga ggtcaatacc ctgcccggtt ttacatcgta
    20821 cagccgctac ccacgtatga tggccgccgc aggaatcacg cttcctgcac tgattgacag
    20881 cctgattaca ttggcgttaa agaggtgata agtatggaaa atggtttttt gtttttagat
    20941 gaaatgttgc atggcgtccg ttgggatgcc aagtacgcca catgggataa cttcacagga
    21001 aaaccggtgg atgggtatga ggtgaatcgc atcatcggca caaaggccgt ggcgtttgct
    21061 ctgcgcgagg cacaaatcca tgcggctgcg cttggctatg gcttgctttt atgggatgga
    21121 tatcggccaa gaactgcggt ggactgcttc ctgcgttggg cagcgcaacc ggaggacaat
    21181 ctcacaaaag aaaaatttta ccccaatata gagcgagccg agttgattac aaagggttat
    21241 gtggcctcac aatccagcca tagccgtgga agcgcaattg atcttacgct ctaccacctg
    21301 gatacagggg aacttgtttc aatgggaagt aacttcgatt ttatggacga acggtcgcat
    21361 catacagcaa aagggatagg ggatgcagag gcacaaaatc gaagatgctt gcgtaaaatc
    21421 atggaaagca gcggatttca gtcttatcgc tttgaatggt ggcactataa gttgattgat
    21481 gagccatacc ccgataccta ttttaatttt gccgtttcat aatgaaagta ttggttttgt
    21541 atcagatttt ggatttgata aaatctatgc tggtcatgga caatctttta ctggaaagtg
    21601 gttaaagcat cttttgtaat acattgcaaa ttatagtatt caatctgccc agcggcagaa
    21661 aagaaaaaaa ccgctgttgg gcagaccttt cccacgccca ttttgtattt tgctggcggc
    21721 ggttttgaga aatcaaggcc gccgttcttt tctggcagca aaaggtgtgg cggcccatag
    21781 gaggatgccg ctatgctgtt ttgctattgc catgctctaa tccacttcca ccactcaaaa
    21841 aaagccttgc ccctccatga actgaacccc aaaagttgaa ctatttaatg gactgt
//
LOCUS       DAZULG010000324        16917 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.guided.062, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000324 DAZULG010000000
VERSION     DAZULG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.guided.062"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /gene="rfbC"
                     /locus_tag="ACG9CY_003042"
     CDS             complement(<1..446)
                     /gene="rfbC"
                     /locus_tag="ACG9CY_003042"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS4939451.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAF"
     gene            complement(460..1326)
                     /gene="rfbA"
                     /locus_tag="ACG9CY_003043"
     CDS             complement(460..1326)
                     /gene="rfbA"
                     /locus_tag="ACG9CY_003043"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4939452.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(1426..2133)
                     /locus_tag="ACG9CY_003044"
     CDS             complement(1426..2133)
                     /locus_tag="ACG9CY_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4939453.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            complement(2138..2965)
                     /locus_tag="ACG9CY_003045"
     CDS             complement(2138..2965)
                     /locus_tag="ACG9CY_003045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4939454.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(2965..3765)
                     /locus_tag="ACG9CY_003046"
     CDS             complement(2965..3765)
                     /locus_tag="ACG9CY_003046"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4939455.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(3758..4897)
                     /locus_tag="ACG9CY_003047"
     CDS             complement(3758..4897)
                     /locus_tag="ACG9CY_003047"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS4939456.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(5102..6295)
                     /locus_tag="ACG9CY_003048"
     CDS             complement(5102..6295)
                     /locus_tag="ACG9CY_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4939457.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(6374..6580)
                     /locus_tag="ACG9CY_003049"
     CDS             complement(6374..6580)
                     /locus_tag="ACG9CY_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4939458.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(6573..7715)
                     /locus_tag="ACG9CY_003050"
     CDS             complement(6573..7715)
                     /locus_tag="ACG9CY_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4939459.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            8227..8862
                     /locus_tag="ACG9CY_003051"
     CDS             8227..8862
                     /locus_tag="ACG9CY_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4939460.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            8998..9090
                     /locus_tag="ACG9CY_003052"
                     /pseudo
     CDS             8998..9090
                     /locus_tag="ACG9CY_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            9204..9839
                     /locus_tag="ACG9CY_003053"
     CDS             9204..9839
                     /locus_tag="ACG9CY_003053"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4939461.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            9946..11184
                     /gene="mef(H)"
                     /locus_tag="ACG9CY_003054"
                     /gene_synonym="mefH"
     CDS             9946..11184
                     /gene="mef(H)"
                     /locus_tag="ACG9CY_003054"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFS4939462.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            11411..11830
                     /locus_tag="ACG9CY_003055"
     CDS             11411..11830
                     /locus_tag="ACG9CY_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4939463.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            11835..12074
                     /locus_tag="ACG9CY_003056"
     CDS             11835..12074
                     /locus_tag="ACG9CY_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4939464.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(12299..13222)
                     /locus_tag="ACG9CY_003057"
     CDS             complement(12299..13222)
                     /locus_tag="ACG9CY_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS4939465.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(13254..14018)
                     /gene="map"
                     /locus_tag="ACG9CY_003058"
     CDS             complement(13254..14018)
                     /gene="map"
                     /locus_tag="ACG9CY_003058"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4939466.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            14176..14616
                     /locus_tag="ACG9CY_003059"
     CDS             14176..14616
                     /locus_tag="ACG9CY_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4939467.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(14794..15363)
                     /locus_tag="ACG9CY_003060"
     CDS             complement(14794..15363)
                     /locus_tag="ACG9CY_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4939468.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            15618..16850
                     /locus_tag="ACG9CY_003061"
     CDS             15618..16850
                     /locus_tag="ACG9CY_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4939469.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         5206 a         3240 c         3074 g         5397 t
ORIGIN      
        1 tcaaatgcga tgcccccatc tgcttctgca ttatagtaat tatcacactt ataaaggaag
       61 ttcacatttg gtgtcaaagt aacgaatcca tgggcatatc ctttaggtac aagcaactgt
      121 cgatgattat gttcagataa gataaatccg tcccattgac cataagtcgg gctacctttg
      181 cggatatcca ctactacatc taaaactgct ccagtgacta cccgaatcaa ttttgtctgt
      241 gcagcttctc ctttttggaa atgaagcccc cgaaggacac cagcttcagc tgatagtgaa
      301 tggttgtctt gaacaaagtc atatttcaat ccatgttctg caaattttgc ttttgaataa
      361 ctttctgtga agaaaccgcg atgatcgcca aagacgtcca tttctacgat tttgacatct
      421 gttaattttg tatctgttac tttcatctgt aaggactcct tattcagcgg ccaaattcaa
      481 taaatattga ccgtattgat tttttttcaa tggctgtgca agggccagca attgttcctt
      541 atcgatgtag cccatacggt aagcaatttc ttccaaacaa gccactttta agttctgacg
      601 tttttcaatt gtttcgataa aggtagatgc ttcaagcaat gattcatgtg ttcctgtatc
      661 aagccaagca aatccgcgtc ccataagttc tacggatagt tttcctttat ccaaatatgc
      721 tttattgaca tccgtaatct ctagttctcc acgttctgaa ggtttgatac ctttagcgat
      781 agaaattacc tcattgtcat aaaaataaag gccagttaca gcgtaatttg attttggctg
      841 agcaggcttt tcttcaatcg acaaagcttg catattctca tcaaattcta ctacaccaaa
      901 gcgttctgga tcattgacat gatagccaaa tactgtcgcg cctgtatctt ttgaagctgc
      961 tcgttgcaac attttagaca gaccgccacc atagtaaata ttgtccccta ataccaagca
     1021 gacactatca tctccaataa attcttctcc aatgataaac gcttgtgcca atccatctgg
     1081 actttcttgt actgcatatt cgatctgcag tcctaaatca tttccgtttc caaataattc
     1141 cttgaaacgt ggtgtatctt gtggcgttga aataatcaga atttctttga ttcccgccaa
     1201 catcagagta gacattggat aataaatcat tggtttgtca taaatcggca tcaattgttt
     1261 cgatgttgcc tttgttaaag ggtacagacg tgttccgctg cccccagcaa gaatgattcc
     1321 cttcatggat atctgctctc ctttttgatt tcaaatttca ctttgttatt atctcatcaa
     1381 ttgttatatt tgtcatggaa atattaagga tttaaacatc ttttatcaac gtttaaacca
     1441 ttttcgtttg atcaacgccg taatcagatg aacacgccaa gaaatttttt gccgtaattt
     1501 agaatgtgtt tcaatctcac tcgcgtttaa atcatgtctt cggtacagag ttaacggttc
     1561 tcttaggaac ccagtcttct ttcctcccag caaaccaatc cacatatcat gcatcggcac
     1621 ttctgacggg atcggcaaaa tatcgggttt taaagaagac cggaaagcca taccagcacc
     1681 aatatagtga cttcttatca cattattaag ccatccttct ttggaatttc gaaacgaaaa
     1741 atacgaatca tggatctctt gcagctcgct gtccacaatg attaaatcag aaactactac
     1801 tagctgatcc ggattctgct caaaatagtc agtgatcttt ttcacttttg tctccaacca
     1861 aacatcatct tggtctgcca aaaagattag atctcctttc gtctgctgga tcgcataacc
     1921 aaaattagcg atcaatcctt gacgaggccc cgaaaaaagc tggattcgct tatctttttc
     1981 tgcgtattca gttaatatat cccatgtatc atctactgac ccatcatcag aaatgactac
     2041 ttcatcttct gctgaaagct gagataaaat actatctaac tgctctctta aatatctgcc
     2101 tccattaaag gtagctatac aaaccgaacg cacgaattca caccttccaa ttcttaacat
     2161 atgagcgcaa agtcatccgc cagatatgcc ggtttttgac ggtcaagaac tgtttagctg
     2221 ttcttaacaa taattgtcgt cggtgactgg ataaatgcct ttgatcatat tcatgataaa
     2281 atcgattttc tgcttgtaaa atagaacggt atcgattact tgaaactttt tcatagtcta
     2341 aaaccgaaag atcatgttcc attcgtacag gaaggatctc cacagttttt tccaactgat
     2401 ccactttcca aaacagccaa tgatccaaaa aatctaaaga aaaagcagga ttaaacccgc
     2461 caatcttttt tagaaatttg acagaccagg caacacctga attgattgcc atgattcgtt
     2521 cagctgttgg gccttcaact tctactgctt gagctgtacg attgatataa cgatctgaat
     2581 ataggggaga aacttgtcgg ctcccagaaa aaatcatcgg aactgcagca acacattcat
     2641 ctgtaaaggt tactttacgc aatagttcaa aataatcgtc gactagcctc gtatcctgat
     2701 ccagagtgac gagacaacgt gttccttcat ctgcttgaga caacgcaaaa ttataagcca
     2761 ccgctaaacc aggattttgc ggatcatgat gataaacgat attttctttt tcaaacagct
     2821 cattttgctg cctttgagga ctattatcgt aaataataag ctgaatttgc ttttcgctaa
     2881 gtgctttgct taatggtcca taagctggag attgactgaa tagttgctga taaagcacaa
     2941 cgactactaa tatttctggt ttcatcagaa aaaacgccac ccccacttat tgaaaaattt
     3001 catcatagaa gtcaaaaaca cgcgaaacag ttttgcattt cgatgcgcac ccttcccgta
     3061 aaaatggata actgcataaa agggtgtgta caataacttg taacctgctt tttcaaaact
     3121 caaacacaag tcgttgtctt caaagtacat gaagaaacgc tcatcaaacc cctttatctc
     3181 tttaaattta tcaatatcag ctaccataaa actacctgag atcatccgta cataactatt
     3241 agcatcagta ggcaatcctc ggcattcaaa cacagccagt cgtttgtcaa actttttttt
     3301 gacccatttg aaaggcacaa agcgcaaaac gtaatcaaaa acagccagct gctcacggat
     3361 gaggtgctga gttgttccat cttcattaag aatcttaggt gctaacatcg cacattccgg
     3421 atgttctttc aataacaaga ccaatgctgt aactgtctct tcatccacta aaatatctgg
     3481 gttacaaata atgccatagc gagaatcgct agctatcaga ttgtgattat gtccataacc
     3541 aaatccggaa ttttcatcat gaaaatagat atcgatgtag ggatatttcc tatactctgt
     3601 tagtcttttt ttgtactcgg gaagtgaatt gttgtcaaaa attcggattt ttactgctgt
     3661 ttcatctttt acagttgaaa taaaattatc tagtgtccga aaaattttat cactgtttgc
     3721 cgtcacaata ctaataagga tcggctggtt taatttgtta ttcatctata tgacggttcc
     3781 atttcttcaa atatttctgt tttacttgcg tacggtactc tttattccct aaaaatcgca
     3841 atgtttttaa aataaactga tgtttctcat tgatcagacc aatcaattca aataataaaa
     3901 ttgcagcaaa caacatggca ataatcaata tgatcgttcc gattcttcca gtataattca
     3961 tgaccatagc tgtgaaagaa aacatcaaag ccatcgcata aatcgtcagg acagcacctt
     4021 tatgagtaaa gcctaaactg attagtcggt gatgaagatg ttttttatct gctgtagaaa
     4081 aagaaacacg gtttgctttt ctcctaatga tagcaaaaaa cgtgtctgtt accggcactc
     4141 ccatgacgat cagcaaagaa atcgatgaaa caaaagtcac gttcttcagc ccttgtagtg
     4201 aaaggacggc catcataaac cctaggaata gagctcctgt atcgcctaaa taaatctttg
     4261 ccggataaaa attgtaagga aaaaaaccga taatacttgc tacaagacag aaaataacaa
     4321 ctggaatata cactgtagaa gcatggagga aaaaatatcc aatgattcct atcgtaatta
     4381 aaccgatcaa tgaaatccct gccgccagcc catccaaacc gtcaatcaga ttgaccgcat
     4441 tcgtgatacc gaagatccaa aaaatcgtta cgggaaaact aagccagcct agttggatat
     4501 atccaataaa aggaagcgtg aaggaatcaa tacgtatatc tgctacaaaa taaataataa
     4561 gagaagcaat caagatccct atactttttt gtttaggtgt cagctcatat ttatcatcga
     4621 gcactcctgt gactatgatt actaaagaag caatcaagat cggtacaata tacgattgag
     4681 ggataatttt acggaataag aataaagtcg atataacaaa tgaaataaag ataggcagtc
     4741 cgcccgctga aggcattgga actttattta ttcttctttc atttggtttg tcgaccatac
     4801 ctgtttgtac tgaaatgatt ttgaatacag gtgttaatat cagtgataat aaaattgtca
     4861 gaaaaaatct tataaccata ctaaaagaaa caagcatatg ggcacctact ttaattaaga
     4921 gtcataatta ttataaatta tatatgtctt tttgactatc tttttatatc ttctcaaatg
     4981 tccgcaaagc cttattttat cggctctacg ggcattttgc ttttgtggta aacctcgcat
     5041 atctaggtct atcttcttat attttccctg tcaatcgtgg ttaaaatcgt ggtaaatact
     5101 tctatgcgat tagacgctga acctctgatt ttgcggtatc tatggacgca tgagcgtacc
     5161 agttcattgt gatactgatg tttgaatgcc ccatgatata ctgtaaatct ttcgggttca
     5221 tgttcttgct tgccagcctt gtgcagaatg tatggcgtag cgtatgcggt gtaatatgtg
     5281 gcaagggatt gtccttgtgg tgcttgttgt atttctttac catacggata aataaggcgt
     5341 tataatcaat ggcaactttt ggattacctt tcggattgat aaacacaaaa ccgctgtgtc
     5401 cgtctacctc tatcgacttt atctttggtc ggctgttgat aacccgttga aatgcctgta
     5461 tcgtttcttt gcttaatggc acttgcctta tcccgctttt cgtcttaggc gtttcaatat
     5521 aatagccctg ttccttactc tttagtaact ggtggtcgat aatcacaact tcattcttga
     5581 aatcaatgtc ggctactgtc agtccgcaca gttccgagat acgaagtcct gtctttaaca
     5641 gtatcagcac atcatcataa tgcttgtgat acacgttgtc cgtcttaata aatgacaata
     5701 aggcttgctc ctgttcctct gtcaatgcga ctttctcttt ggtatcattt tctatgactt
     5761 cacttagctt gaaatcaaaa gggtttttcc tcacataatc atcttgtatg gctatgtaga
     5821 atgacgcttt taacgagcgt ttatggttgt taatggtatt gtaggaaaag cctttgtctt
     5881 tcatgcgtaa cgcccattct ttagcgtcag agggttttat cgtatcaatg ctcctagcac
     5941 ctaacttgtc ctcttttaat aaccgcatga gttgttcccg ttgtttctgt gtgcttttct
     6001 tcacatttgt cctctgtgcg ttctgtttgg catagagttg gcaaagcgtc attttcttgc
     6061 ctgtgctgtc gataccgtcc tcaatatccc gtcttatctg ctgttccagt tctcgaagtg
     6121 agggtttttc ccgttttccc tttggtgtcg ggtctgtggg tgtcaatctc caagcataca
     6181 catattttgt gtttcccaat gcgtctacat atttatataa gtattttccg tctgttcgtt
     6241 ggctctctcc agtatgcagg atacgacctt tgttatcccg tcttttttct ttcatggtgt
     6301 ctgctccttt ccttgttgga aagagccttg atatgacttg tgttcatcat aacacataca
     6361 aggctcattt gcattagatt gcgtccaatt tatcaataac cttttcaaac tgtcggcgtt
     6421 taatctgtat gcggttgcca ttcataatga gccagccagc gtccttgttt tcctctgcca
     6481 atcgtcttaa cttgttttct ccaatgcgga aatactttga cgcttcttca atggtaaggg
     6541 tgtatttttc ccatacggga atatcctgtg tattactcat aaaacacccc ctcactttcg
     6601 attacgtctg atatgtccgt acacatctgt tccacaagca tttcctgttt ttcagaaagg
     6661 tgggtacatt catcaatcat tttcatgtag tttgaacctg tcagcctgtc gatttcctct
     6721 atcatcacga ttgacggcat gacctgtctt tccagccatt gcagagtgcg ttccatattg
     6781 acaggtacag ggttcatttc aaaaggtaaa ctgtcatggg aaatgaatat ctcccacaat
     6841 ggatagtcag gaaattccac aaaatcggta atgagataaa acaccagccc atgagggtta
     6901 tacgtcaata acagttcttc aactgcctgt accgcctttt tgtcacgcag acgaatttca
     6961 aaacggttga taatatcggt atgtttcttt ttcgttgcct gttccttttg tttttcatac
     7021 aggcagaaat attttgtgct tgcctttgag ccgatataaa gggtacttgc taaattacgg
     7081 ttttttccgc ttaattttcc accttgtaca ttttcatagt ttttacagcg acattccgca
     7141 ccaccatttc tgtatttttc agatagcaca ggaatatcca gcagaccgca catatcattg
     7201 attgccaagt caaaacgtcg gataacaccg ccacagtcaa gacagcggtt taagaatgaa
     7261 taccagtccc tgttctgtgc ctgtaagaca tattccatgt tgcgtgagcc tgcccctttt
     7321 aattctaaaa atacgcccat gtgttcatca tcagaagcca tgaccttgat ttccccgtaa
     7381 gcataatgct ccttatagcc ataataccca tagtcataat ggataaaata atctgctttc
     7441 atgccaagca cctttcggat aacttccaat gcgtcagtcg ttggaaaacg gacagacaga
     7501 taatcaacga gcagataaaa aggcttatcg cagatatagt aattcagaca acgcaggata
     7561 atgttctgtg tttccttgct ggcgttagct tttccgttct caaagcggtt gatactatat
     7621 cttgatacat gggtaagact tgcaagttct ccttgtgaaa gaccgctggc aagacgtttg
     7681 ttcttcattt caagtgcaaa ttctaaattg ttcattgtat tcctccaatc ccacaatagc
     7741 cttgaaatac ctgtaaacca cctttcaaaa agtgtgatat gtactatctt cttaaaacct
     7801 ctcattacta ctcacaggtt tgggggtgta tggtttttga cttgtctttt gtgaaatagt
     7861 gcccccctgt tagataacgg gggcacagac aaggctcgca ggctcgccag ccgacaaagc
     7921 atttcttccc cttgacacaa ggtgtcatgg cggtaagcct gagaggcttc cgccaccgtg
     7981 tcagaaatac tttgtcagca agttctgttt tgccttttct aacaagttcc atttcaaccg
     8041 atattcatat ctacggattt aatcagatag gtatttcttt ttgaatgttt ttatatttca
     8101 ttctattgtg cttattcgat tacaagaaca tagtaattct tatatgctta tttgtcaata
     8161 tctttttttg cctttacaaa tcatattaag catgatagac ttataatagc gaagatataa
     8221 cgagatatgg agggttatgc aatgaaaata agccatttag gaaataatat acagaccata
     8281 agaaaattta ggggaatgaa acaacaggaa cttgcggaca aaatcggtat caatatgcag
     8341 agcctttcca agattgaaag agggttgaac tatcctgcct atgaaacgct ggaaaagata
     8401 atggaagtgc tggacgttac gcccaatgaa ttgttgtcgg gagaatggaa gtatgtcaat
     8461 caatctgaaa aggaagtctg tcagttttta agagccgaag aacgtctaaa tgcagaactg
     8521 aaacatggac actatgataa cttctttgac agcgaggaag aatggctgga atatgagtta
     8581 gaaaagttac gggaatatat tacagactat atcaacggga aaagcattga agcgtccgac
     8641 ctttacccaa tcaaagaatt tatccagcat ttgaagtttc aaaaattatt agaccgctat
     8701 gatgatttat acagtatgga tatgtttggg gaaagcatag agggacataa gtataagaca
     8761 ccttatcaag tcgtgaaaat gataaacccg aactcaaaag aagatatgga actgttacga
     8821 gaagtattaa ggaataacaa ttttgatgat gaggacgagt aacttttttc ctcttgcgag
     8881 aagatacagc aaacaaaaag cccgatcaag agtgcaaagc accaccaatg gtggcaaagc
     8941 tcttgacagg gctttttctt ttctgttgtg ggcaatcaag aggaagaaag ggataaaatc
     9001 tgatttgtgg taaatcaggc aaaggcaacg caggtatcac agtatgaatt gacaccgcat
     9061 aaggaaagca actggaagat tgtagaataa atgaaaaagg cttgcattct gcatttttat
     9121 aaaattgcaa gtctttatat tgacaagcat aaaatactaa gatatacttt aatcagacca
     9181 acggtttgta ttggaggtaa gttatggcac gaaataaata tccagaagaa acagtaaaac
     9241 tcattttaga tgtttctaca aaaatgtttt gtgaaaaagg atatgataac acatcattgc
     9301 aagatattat caatgaaaca aaactttcta aaggtgctat ttatcatcat ttcgattcaa
     9361 aggaagatat tttaaaagca atttttcaaa gaattggaaa tgaaaatgca gtaatatttg
     9421 ctaaaattag agataacgat agtttaaacg gtttagaaaa gttaagagaa atatttaaga
     9481 cagcggtatt caattctaac caaagcgttt tattgacagt ttctcctcaa ctattaaaca
     9541 atcctcgttt tcttgctatg caaatagaac aaatgtatga aattgtagta cctgaatttg
     9601 ttgaacccgt tttagaacaa ggtatcaagg ataagtcttt ggagattgaa aattcacatg
     9661 aaattgcaga agttatcatg gtattaagta atgtatggct caacccatta gttgaaatga
     9721 cggataaaga gggtatggaa aaacgttgca atacattcaa tgccctttta aatggaatag
     9781 gcatagacac attattagac gaagaaacaa tttctgcatt tatacagttt tgtaaataac
     9841 tgtatttgtt atccctatac gatagactgc tctataaaag agcagtttat ttaaaccatt
     9901 atataaaccg acggtctgtt tgattaaata aaaaggagag attttatgca agcagaaaaa
     9961 aaacttttta atcgtgattt tatacttgtt gttataggac agataatttc tttatttgga
    10021 aacgctatcc tacgatttgc attaccgtac tatttgttaa aagaaactgg ttcatcagct
    10081 ttgtttggta ttgctacagc ctgctctttt ttacctatgg ttattttgtc gctggtaggt
    10141 ggtattattg ctgacaggac aaataaaaga aacattatgg tcatactaga ttttgtaaca
    10201 gcaatcataa ttttagtatt ttatttgtca ttgggaaaaa ttcctatcat acctttgttt
    10261 attgttgctt tgatgttact ttatggtatt tctggtgcat atcaaccagc ggtacaagca
    10321 agtattcctc tattggtatc atcagataaa cttaccgtcg gtaatgctat aataaatcaa
    10381 gtaaatactt tagcaaactt tatcggacca gctataagcg gagtagtttt tcaattttat
    10441 ggtatcaatc ccattttaat tgttagtatt atatgtttta ctttttcagc cattatggaa
    10501 atttttatcc atattccaca ccaaaaaaga cctcgtgaag taggtgtaat tgcagttgct
    10561 aaaaacgatt taaaagatag ttataccttt ttgaaaaagg agaaacccat ttttatatcc
    10621 gtaatctttt tggtttgcat tttcaatttg gttttaagtg ctgttttaat tgttggtctg
    10681 cctatcttaa tttctgaagt tttaaaaatg ccagatacac tatatggttt ttcacaatca
    10741 gcattatcat taggtggttt atgtggtggt attttaacag ctcttgttgc ttcaaagtta
    10801 aaactaaaaa acctttatac cttgttactt atttgttctg tttctgtttt acttatggga
    10861 atttcgctat ttttagaatt gcctgcaata gtttcctact gggtagttac agtgatgtgc
    10921 tttgtggcta tgggtgtatc tactatgttt gttgttcaaa tttacactct tgttcagaca
    10981 caaacaccac cgcaattagt aggaaagata atggctacgc tggtagcagt tgctatgtgc
    11041 ggtcaaccta ttggacaaat ggtttacggt attttattcg atgtatttat acaagaaaca
    11101 tgggttgtta tgtttgtagc ttctattgct tcaatcatga tttcaatata ttcaaaaaga
    11161 atttttcaaa agttagagat ataatacaag gggttaaata gtatgttagg ctaaaaacaa
    11221 tatataaaag aaaatttgaa atctgctaaa aggatagtgt tataaaagaa agagtaatat
    11281 tgcagtattt aaggctcgca caaatatatg ttttgtgtgg gcttttttct taccattttt
    11341 ctgtatttct aaagggtata aacggtattc cgtttatata tttcagcatt agcagaaagg
    11401 gggtgagatt atgaggacat cttctttcga gcatatcgtc agattacagt tcaattcttt
    11461 aatgctgatt gtcattaaga acaaagtgaa aagtcgaaac agacagcttg caagacattc
    11521 taaacgtgaa gtattgtttt gtgaaatagc cgaaacgaaa caagttgaac gtggaacgat
    11581 tgatacctgt ttttatgatt gtgtatcatt taaagtcttg cactttacaa tttatgtatc
    11641 tgatgaaaca cttggtacag ttttacatag actgtcagaa aaacagcgta gtgcgatttt
    11701 attacgttat tttcaaggaa tgaacgacag agaaatttca gaactgtacc atgtatcacg
    11761 ttctgctatt tgcagtagaa gaactagagg actaaggaag ttaaaaatgc ttttgagcga
    11821 aaggaagtaa agaaatgagt aaagattatg gctatccctc ttttgaattg atttgcaaag
    11881 cgtcaagcgg agatgaaatg gcagttaaag agattttgaa gttttatgac ggttatattt
    11941 ctaaattatg cttgcgtcct ttttaccact ctgaaagtgg taaaatcatt atgcaggttg
    12001 atgaagaact aaaaggagaa attcatacag acatgatgaa agcaatattg aaatttgaaa
    12061 tcggagtgaa gtaatcaact gtataaaaaa atcgggagat acacagtttc gtgctatctc
    12121 ccttagtagt tttacggtca tatcaaggct cattcaatcg tggtaaattc gtggtaagat
    12181 gagtgttttt ctatacttca aaatgcttga atgtatagtg tttatgcgga taattgaaaa
    12241 ttagatataa aatttcaaat aaattactga tataaattta caataagaac tcttgtttct
    12301 actttttttt atgagaaaat ttagttttca atttattgaa ccattttcgg cttggatctc
    12361 gacgtttttt gatttgatga cccgtcacgt attcttgaac aactgcattg atgattccgc
    12421 ccaaaatgat gatcgtagca gcaaaattaa gccaaagcat gagtacaata aaacttccta
    12481 tgatctgata accgctgatc ctcgttgcaa aatacctagc atagatacta aacacttgtg
    12541 ataacagcat ccagccgaca gtagcgaaga tcgtgcccgg aatgatcgtc ttcaacttca
    12601 actgagcatt agggacaatt aaataaatca agaacatgat gacaaataaa gctaccaatg
    12661 tgattggcca cttcaaagtt tggaactgat cgataaagtt cacgggtatt tgtacgattg
    12721 gctgaatagc atccaaaatc agctgtccca acccaagaac gatagttacc ccactaatag
    12781 caatcatgaa caagacaatg acaattagtg agaagaaacg gacgatcaaa aaattttttc
    12841 tattttctac gccatacgct ttattcatcg caatttgcaa agcattgata ctttgacttg
    12901 cagaccataa agccgctaat gcagaaacgg ataacaagcc tcctgaactt tgtgtcaaaa
    12961 ggtctttgat tgcaggaccg ataaactggt aaacggtctc aggaatcact tctcgtaaat
    13021 aggtcagtac agtctcttga tcgatgtgga gaaacggtaa aatattacca aaagcaataa
    13081 tcaatggaaa taaagacaag agtaaataat aagcaacgac gacagaggtc gtactcattt
    13141 ctgaatcagt gatgcgcttt tgagtcgtct caataaagcg catcagattt tcattttgtt
    13201 ttattttgtt ccaaactttc atgtacctct cccgtaccta ttgactggat agatcaataa
    13261 gtgccttctt ctccttgtga tgtcaaaatt cttggaccat ctttagtaat agccaatgta
    13321 tgttcatatt ggcaagaaag tccgccgtct cttgtatacg cagtccagcc gttaggatcc
    13381 attttcattt tccaagttcc ggtattaacc ataggttcga tcgtgataac catgccttct
    13441 ttcaaccgca acccttttcc agcttctccg taatgaggaa ctgctggttc ttcatgaatc
    13501 gtaggaccaa cgccatgacc aataaaatca cgaacgatcg ctagattttc gccttctaca
    13561 taagtttgga tagcatgacc gatgtcacca atccgattcc ctacttgtgc ttgctcaata
    13621 ccgatgtaca aagctttctt cgttacttcc atcaatcggt cgatctcagg tgtagattct
    13681 ccaactacat aggcccaaca agaatcagaa atgccgcctt tcaaatcgat acacatatcg
    13741 acttttacca aatcaccgtt tttcaaaggc tttttacgag ggaatccatg acaaatttca
    13801 tcatttatac tgatacaagt cgcatattca tacccttcaa agccaatttg cgcagcaatt
    13861 ccgccatgac tctcgatata gtttctgaca aatacctcaa tatcccaact tgtgattcct
    13921 ggttttataa aatcacgcag atttttatgt acatctgcta ataaagctcc agattctgcc
    13981 atcatttcaa tttcacgtgc tgattttaaa gtaatcatat tttcgtctcc tttttacaaa
    14041 tccctctcat tttaacatag cggcatacat ttagccaaat gacattcccc aggtgtctct
    14101 aggttttatt ttcttttcaa tgatgttttg gtataattca taattgttaa ataattataa
    14161 acgaggaggc acatcatggc tttagcaaaa atcgtatatg ctagcatgac aggaaacact
    14221 gaagaaatcg ccgatattgt tgcagaagca tttgaaaatc tggatatcga agtcgaaatc
    14281 aacgaatgta cccaagtaga tgcagatgaa ttcgaggagg cagatatttg cgtagtagct
    14341 acttatacat acgatgatgg cgatctgcct gatgagatcg tagactttta tgaagacttg
    14401 caagaattgg atctattggg aaaaatttac ggtgtctgcg gatctggtga tactttttac
    14461 gatgaattct gcaaatcggt ggatgatttc gatgctgctt ttgcaaaaac aggcgcatca
    14521 aaaggagccg aaaatgttaa agtggatctg aatgctgaag aagaagatat cgaaaattta
    14581 gaagctttcg ctaaagaact agtcgcaaaa atttaatata cttgcaataa aagagccatc
    14641 cagatttatt caatctgtat ggctctttta ttcattgcat tcatggaaga gttcttcggg
    14701 agttaaaaat gcatacgctg taagaaaaac gaacttttcc ttattttgac acatctcttg
    14761 ttacctactg atttgtggaa cttttgagta tattcaatcg atttcgattg gttctttgaa
    14821 tttcaagata gcatctatac tgtcttggat catcttttca atctgttcat cagttggatg
    14881 gatgatgctt gaagacatca aagctgcgat cccagtagct atgatccatg tcccattata
    14941 caaagattcg atctgctctt ctgaaagatt cacataagct ggatcgcgtt tcactgtatt
    15001 cacaaagtaa gtataagaga aattctgcat ctcttttcct ccaccatatt cttctaagta
    15061 cagtgaacga tacaacttct tctctcttct tgcaaaatgg atataattaa aagcaagatc
    15121 gataatcgta ttccctgtat gctcaacagt gaacacttca tattgtaaat gatcatagat
    15181 ctgctcaaaa agagcttgtc tcaagtcatc catattttta aactcaagat aaatcggttg
    15241 cgtggaacaa ttcattttgt tcgcgatgtt tctcgcagta aatcgtgtaa atccatcggt
    15301 ggcagctact tcataagcag ctgccaaaat ctgttctcta gtaatcgtct tttttcttgc
    15361 catatttttt aatctcctta atttttttat aacgcttcca ccattgatta atttcattgt
    15421 aacatgtttt ttttgatatg caaaattagt ttttgatttt cttcacaagc tgaaatagca
    15481 agtagtaaca tatatcatga aacttaacct taaaaaaata acctcctttt tttatttaaa
    15541 gcctcattgt ttgaatcata attaaatggt atcatgctaa tagttaccag aaaaaatggt
    15601 gaggaggaat ttttattatg ttatctgatt ttaatgaact tctaaaaaaa tatgcacgct
    15661 taattgcaga aactggcgta gccacagaaa aaggacacac agttgtttta cagatcagcg
    15721 tggaacaggc accccttgct cgattgatca cacaagaagc ttataaacta ggagccgctg
    15781 aggttatcgt tcaatggact gacgaccaga tccaaagaga atttctttta catgctgcaa
    15841 cggatcgtat tgagaatgta ccacaatcta aaatcgatca agcggatgaa tggcttgaaa
    15901 aaggagcaag caggatcagc gttgtttctg ccgatcccga tgcatttgct ggtgtagata
    15961 gccatcgagt agcgtcttat caatcagcga atggcaaagc actaatgaat ttacgcaagg
    16021 caactcaagc aaataaagtc agttggactg tcgtggctgc ggctggtaaa caatgggcag
    16081 caaaagtctt cccagaactg ccagaagaaa aacaagttga ggcattatgg gatcagattt
    16141 tcaaaacgac tcgtgtctat gaagaagacc ctgtcttagc atggaagaag catgatgaaa
    16201 aattagcaaa aaaagcagaa gaattgaatc gagaacaatt ttctgccctt cactatactg
    16261 ctcccggtac agatatcatt atcggattgc ctaaaaacca tctttgggaa ggcgctggaa
    16321 gctataatgc tcgtggagaa aaattcatgg caaatatgcc gacggaagaa gtgttcacag
    16381 cacctgacag tcatcgagta gatggttaca tttcaagtac aaagccactg agttatgctg
    16441 gcaccatcat ctcgggtatg aaatttactt ttaaagatgg aaaagttgtt gatttttctg
    16501 ccgaacaagg agaagatgtc ttagcaaaac tgcttgatac agatgaaggt gcgcgtcgtt
    16561 taggagaagt cgcacttgta cctgaccctt cacctatttc tcaatctggc atcatctttt
    16621 tcaatacatt atttgatgaa aacgcatcga atcacttagc actaggttct gcttatgcct
    16681 tcagtgtgaa aggcggtaca gaaatgtccg atgaagaatt agcagaagct ggattgaata
    16741 gaagtcaaac ccacgtagat ttcatggtag gatctgacaa gatggatatc gacggaatcc
    16801 gtgaagatgg ctctacagta ccgattttcc gaaatggcga ttgggcttga gtatactttc
    16861 gcacacatgc aaaaaataat ggctctttgt caataaggac tgatgagttg tgcaaaa
//
LOCUS       DAZULG010000325        10087 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.guided.097, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000325 DAZULG010000000
VERSION     DAZULG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.guided.097"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            353..523
                     /locus_tag="ACG9CY_003062"
     CDS             353..523
                     /locus_tag="ACG9CY_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939470.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            537..1139
                     /locus_tag="ACG9CY_003063"
     CDS             537..1139
                     /locus_tag="ACG9CY_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4939471.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1155..1832
                     /locus_tag="ACG9CY_003064"
                     /pseudo
     CDS             1155..1832
                     /locus_tag="ACG9CY_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2143..2640
                     /locus_tag="ACG9CY_003065"
     CDS             2143..2640
                     /locus_tag="ACG9CY_003065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFS4939472.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2641..3057
                     /locus_tag="ACG9CY_003066"
     CDS             2641..3057
                     /locus_tag="ACG9CY_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFS4939473.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3077..4621
                     /locus_tag="ACG9CY_003067"
     CDS             3077..4621
                     /locus_tag="ACG9CY_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4939474.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4764..4988
                     /locus_tag="ACG9CY_003068"
     CDS             4764..4988
                     /locus_tag="ACG9CY_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4939475.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5003..5872
                     /locus_tag="ACG9CY_003069"
     CDS             5003..5872
                     /locus_tag="ACG9CY_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4939476.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5853..6587
                     /locus_tag="ACG9CY_003070"
     CDS             5853..6587
                     /locus_tag="ACG9CY_003070"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4939477.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6620..7528
                     /locus_tag="ACG9CY_003071"
     CDS             6620..7528
                     /locus_tag="ACG9CY_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS4939478.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7525..8067
                     /gene="sat4"
                     /locus_tag="ACG9CY_003072"
     CDS             7525..8067
                     /gene="sat4"
                     /locus_tag="ACG9CY_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS4939479.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8160..8954
                     /locus_tag="ACG9CY_003073"
                     /note="aph(3')-IIIa"
     CDS             8160..8954
                     /locus_tag="ACG9CY_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4939480.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9230..9361
                     /locus_tag="ACG9CY_003074"
                     /pseudo
     CDS             9230..9361
                     /locus_tag="ACG9CY_003074"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9437..9930
                     /locus_tag="ACG9CY_003075"
                     /pseudo
     CDS             9437..9930
                     /locus_tag="ACG9CY_003075"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         3548 a         1572 c         2207 g         2760 t
ORIGIN      
        1 acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt
       61 tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta attaattatt
      121 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
      181 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag
      241 ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta
      301 taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa
      361 atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata
      421 tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa
      481 agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg
      541 ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag
      601 cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc
      661 cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat
      721 tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta
      781 agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt
      841 taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac
      901 gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat
      961 tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt
     1021 tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa
     1081 cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa
     1141 aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct
     1201 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     1261 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac
     1321 caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg
     1381 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac
     1441 ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata
     1501 tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat
     1561 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     1621 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1681 tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta
     1741 cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag
     1801 caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt
     1861 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
     1921 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
     1981 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
     2041 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
     2101 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
     2161 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
     2221 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga
     2281 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
     2341 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
     2401 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
     2461 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
     2521 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
     2581 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
     2641 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
     2701 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
     2761 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
     2821 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     2881 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     2941 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     3001 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     3061 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     3121 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     3181 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     3241 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     3301 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     3361 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     3421 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     3481 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     3541 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     3601 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     3661 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     3721 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     3781 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     3841 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     3901 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     3961 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     4021 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     4081 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     4141 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     4201 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     4261 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     4321 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     4381 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     4441 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     4501 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     4561 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     4621 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     4681 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     4741 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     4801 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     4861 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     4921 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     4981 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     5041 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     5101 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     5161 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
     5221 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
     5281 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
     5341 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
     5401 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     5461 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     5521 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
     5581 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
     5641 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
     5701 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
     5761 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     5821 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     5881 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     5941 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     6001 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     6061 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     6121 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     6181 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     6241 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     6301 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     6361 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     6421 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     6481 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     6541 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     6601 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     6661 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     6721 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     6781 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     6841 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     6901 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     6961 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     7021 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     7081 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     7141 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     7201 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     7261 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     7321 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     7381 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     7441 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     7501 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     7561 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     7621 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     7681 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     7741 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     7801 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     7861 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     7921 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     7981 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     8041 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     8101 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     8161 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     8221 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     8281 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     8341 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     8401 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     8461 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     8521 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     8581 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     8641 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     8701 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     8761 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     8821 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     8881 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     8941 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     9001 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     9061 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     9121 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     9181 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     9241 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     9301 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     9361 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     9421 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     9481 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     9541 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     9601 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     9661 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     9721 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     9781 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     9841 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     9901 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     9961 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
    10021 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
    10081 ttgtgat
//
LOCUS       DAZULG010000326         8182 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.guided.115, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000326 DAZULG010000000
VERSION     DAZULG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.guided.115"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG9CY_003076"
     CDS             complement(134..577)
                     /locus_tag="ACG9CY_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS4939481.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG9CY_003077"
     CDS             complement(795..2135)
                     /locus_tag="ACG9CY_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4939482.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG9CY_003078"
     CDS             2404..3300
                     /locus_tag="ACG9CY_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4939483.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG9CY_003079"
     CDS             complement(3450..4391)
                     /locus_tag="ACG9CY_003079"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4939484.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG9CY_003080"
     CDS             4517..5638
                     /locus_tag="ACG9CY_003080"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4939485.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG9CY_003081"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG9CY_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4939486.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG9CY_003082"
     CDS             7465..7992
                     /locus_tag="ACG9CY_003082"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4939487.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2720 a         1515 c         1611 g         2336 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataaaaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg ga
//
LOCUS       DAZULG010000327         4210 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.guided.169, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000327 DAZULG010000000
VERSION     DAZULG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.guided.169"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..1014
                     /locus_tag="ACG9CY_003083"
     CDS             322..1014
                     /locus_tag="ACG9CY_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4939488.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="ACG9CY_003084"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="ACG9CY_003084"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4939489.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="ACG9CY_003085"
     CDS             complement(1910..3862)
                     /locus_tag="ACG9CY_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4939490.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4105)
                     /locus_tag="ACG9CY_003086"
     CDS             complement(3869..4105)
                     /locus_tag="ACG9CY_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939491.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1426 a          697 c          700 g         1387 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa
//
LOCUS       DAZULG010000328         3043 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.guided.191, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000328 DAZULG010000000
VERSION     DAZULG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.guided.191"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..83
                     /locus_tag="ACG9CY_003087"
     CDS             <1..83
                     /locus_tag="ACG9CY_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4939492.1"
                     /translation="LLDKLNTQLCNITTETKEVNNNGTKK"
     gene            67..696
                     /locus_tag="ACG9CY_003088"
     CDS             67..696
                     /locus_tag="ACG9CY_003088"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4939493.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1050..2528
                     /gene="msr(C)"
                     /locus_tag="ACG9CY_003089"
     CDS             1050..2528
                     /gene="msr(C)"
                     /locus_tag="ACG9CY_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4939494.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2575..3021
                     /locus_tag="ACG9CY_003090"
     CDS             2575..3021
                     /locus_tag="ACG9CY_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4939495.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
BASE COUNT         1040 a          495 c          647 g          861 t
ORIGIN      
        1 cacttttaga taaattgaat acccaacttt gcaatataac aacagaaacg aaagaggtta
       61 ataataatgg aacaaaaaaa tgatttttca acaattatga ctggtagaag atccgttcgc
      121 gtatatgatg aaacatacaa aatcccacac gaagaaatgc tggaaatgat caaagaagcg
      181 acgactgcac cttcttctgt caatatgcaa ccatggcgct ttgtggtcgc tgaaagcgag
      241 gaagcaaaag aaatcttgcg tcctttgatc cgtttcaata cgagacaaaa tgatacttct
      301 tctgcaatgg tcatgatttt tggcgatatg caatgttacg aatatggcga agaaatctat
      361 aaccaagctt acgaatcagg gaagatgcca aaagaagtca gagatcaaca attagctgcg
      421 atcattcctt attacaagag tttttcaaga gaagaaatga acgatgtagt caaagtcgat
      481 tctagcttag cggctatgca attcatgtta gttgcgcgtg atcatggcta tgaaacaaat
      541 ccaattggtg gagtcgaagc agatcagttg gcagaggcat ttggtttgga taaagaccgt
      601 tatgttccag ttattatttt atcaatagga aaagcggtag aagaaggtta cgagtctgtt
      661 cgtttagcac cagaaaagat cactacgttc gaataaaagg ttttcagtta ctattcagta
      721 gctattgctt tttttgaatc gtgtgtggta acctaataaa aaaggaggtc ggaaaaatga
      781 ctgcatcgat gaaattacgt ttcgaacttt tgaataacaa ctaaataaat tcaaaagaca
      841 tgtataaaca acaatttcat ggatggagta tgtccatttt ttcgaacgac agtagaatag
      901 gtgaaagcta tgacagattt ggtgatttag tcatagttat ttcgttcaga gtaggaaaag
      961 atgtgatgca gtttttttgc aaacagattt ttctgctctt ttttagtatc gataattgtt
     1021 gttttacatg aaataggagt gtaaaacata tggaaaattt agcagtaaat ataacaaatc
     1081 tacaagtcag ctttggcaac cagctagaac tatctattga ttctcttcgt gtctatcagc
     1141 aagatcgtat agggatcata ggagaaaatg gtgtcggtaa atcgactttg ctcaaactaa
     1201 tagccggtga actttttccc gatcatggga aaatccaaac agagatcacc ttcaactacc
     1261 tgcctcaatt aacctatctc gctgaagcaa aggacctaaa tttggaatta gccagtcatt
     1321 tccagttaag actggaagaa acttcagagc ggaaatggag cggaggggaa gaacgaaaga
     1381 tcgagttgat acgtcttctt tcttcttatg aacaagggat gcttctagac gagccaacaa
     1441 cccatctaga tagaaaaagt attgatcgac tgattgaaga gcttcgttat tattatggta
     1501 cgctggtttt tgttagtcat gatcgctatt ttctagatga gctagcatcg aaaatctggg
     1561 aagtaaaaga cggagaaatc cgagagtttt cggggaatta tagtgcctat ctcactcaaa
     1621 aggaattgga gaaaaagact cagctacgag aagcagagtc gatcatgaaa gagaaaaaac
     1681 gattggaaaa atcgatccaa gaaaagaaaa aacaagcgga aaagttggaa aaagtgtcca
     1741 gtaaaaagaa aaagcaacaa atcagaccgg atcggttgtc ttcctctaaa caaaaagaca
     1801 gtgtacaaaa agccatccaa aagaatgcga aaacattaga gagaagactc caaaaaatag
     1861 gagaaacaac caagccgcag cagatgaaac aaatccgttt tccagtacca aaatctcttg
     1921 aactccacag ccgctatccc atcatgggac aaaatgtcca attggaaaga agtgggagaa
     1981 cattactggt aaatggtgat tttcagtttt ctttaggtaa aaaaatcgcg attgtcggtg
     2041 aaaatggttc aggtaagaca accttattag aacatatccg caaacaagga gaaggaatcc
     2101 ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa gggttatcaa atgacatctg
     2161 aagaatccat cattcgtttt gtcatgagac aaacagagtt ttcagaatcg cttgtccgta
     2221 gtttgctgaa tcacttaggg tttgctcagg aaactttgac gaaaccgtta tgtacattaa
     2281 gtgggggaga agcgacccgt ctgacgattg ctttgctttt tactaagcca agtaatgtgt
     2341 tgctgttaga tgaaccgact aattttattg atatggcaac gatcgaagct ttagagaagc
     2401 taatgcaaat atatccggga acgattttgt ttacttctca tgattcttac tttgtcgagc
     2461 gtacggctga tgaagtttat gaaataaaag ggcagaaaat aaaaaaagta cttacgagaa
     2521 atttttaaat gtgctacgat gattttcgtg acaaatttaa aggagtggta cagattgcct
     2581 acaaataaaa aagaaggaat aatttttaca acaatgatgt gttttttgat ggtcttgggg
     2641 atgagcgcgt ataatttatg gctgcatcaa gatcttcatt tatcaagttt gttgattggt
     2701 ctagtgcctg gattcgtggt ggctttcatt ttggatgtct tcatcatcgg tgtgctggcg
     2761 aaaaaaattg cctttaaact accagtaaat ccagaaagca aggtccaaat gatcttaact
     2821 atttcatgct tgatggtttt aggaatggtg acatgtatgt ctttattcgg tgtgatcatg
     2881 gaacaaggaa tacctgatca attattctct gcttatatga atgcttggaa aatgaatgtg
     2941 attgccgctt taccgttgca gttgatcatc gttggcccgc tttcaagaag tgtgctaaaa
     3001 gcaattcaaa gtaatgaatg atggaacact gttatttgaa aaa
//
LOCUS       DAZULG010000329         2772 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.guided.198, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000329 DAZULG010000000
VERSION     DAZULG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.guided.198"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACG9CY_003091"
     CDS             111..194
                     /locus_tag="ACG9CY_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS4939496.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG9CY_003092"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG9CY_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4939497.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..2182)
                     /locus_tag="ACG9CY_003093"
     CDS             complement(1319..2182)
                     /locus_tag="ACG9CY_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4939498.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2184..2456)
                     /locus_tag="ACG9CY_003094"
     CDS             complement(2184..2456)
                     /locus_tag="ACG9CY_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4939499.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2473..2682)
                     /locus_tag="ACG9CY_003095"
     CDS             complement(2473..2682)
                     /locus_tag="ACG9CY_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4939500.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          488 c          443 g          990 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattcagggg tttcttggtg ttgattttga accatttttt
     1441 gctctattct ctccaaagtc ggttgaattt ctttccctga tactttacga tttaattctc
     1501 tttctaaggt ctctttcgga ctaatggaag gcgtttctaa gcttgaatag agtttcactc
     1561 cttctctatt gtaaagcctt atatcgctaa ataagcccgt tttatgaagg gtttctaaat
     1621 tggtcggtaa gtttttgaca acaatatcat gcgcttgttt tggtgttgcc ctagctgtca
     1681 ttggatcatc tgcatacatg gtttcatatc gttcaatcgt tcctaagtat gattcaattt
     1741 tcggtactgc catgacgtat atttttgttt cataaccttt agattgaagc attgtggctg
     1801 tttgaatggg aacgtctgtt gttcgtcctg ttccttcaat cactaaatta tagccttgat
     1861 cgcttaaacg gctaataagt gcttctgtca tgcgattcga ataaggggta acgtgtttta
     1921 ctacgtcttt ttcatagagt ttcactaatt cgtcaaaatt tgggtgctgt tgtttaaagg
     1981 tatcattatc aatgacaata acattccctt gtgtttcttc aaaaattgct gatcgcaaac
     2041 tggttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgcta
     2101 gtctattttt agttagtcgt tcaacattct tttctaaacg atcttcaaat tgcttttcgg
     2161 tgaagtcagt aatattcgcc atgttaagcc actttctctt tattcaaaat ttgttttgaa
     2221 tattgattca ttgaccgcca atactcatga atagcttgta attcttcaaa tggttgttga
     2281 aataaatcaa cttcttgtgc cacttctaat tgatttaaaa gattcacttc tagtaaacga
     2341 gtatttttag tatctagttc atgattcaaa acataattta atacccgatt ataaacactt
     2401 gccgataact cattaatgat ttcaatttca aatgtttttt catacgtaac tgccatttca
     2461 ttttcactcc ttttaaagtt tgtcaggtaa atatttccgt atatattctt ctaaggcttg
     2521 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta tgatatggtg
     2581 caagtcggca cgaacacgaa ccgtcttatc ccccattata tctttttttg cgctgattgg
     2641 cgtatcattt cgttttgctt tctgtgcgcc taaatttccc acgatcgctc acttctttct
     2701 atttcttctt actatttatt ttatcatcac ctatcacaaa tcacaagtga tttgtgatta
     2761 atcacttgtg at
//
LOCUS       DAZULG010000330         1671 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.guided.235, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000330 DAZULG010000000
VERSION     DAZULG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.guided.235"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..341)
                     /locus_tag="ACG9CY_003096"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACG9CY_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACG9CY_003097"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACG9CY_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1055..1345)
                     /locus_tag="ACG9CY_003098"
     CDS             complement(1055..1345)
                     /locus_tag="ACG9CY_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4939501.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          547 a          312 c          233 g          579 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagattt aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag
     1561 tatcaaattg caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta
     1621 atttacacca caaaatgtaa gttaaaatgt atataaaaat gcgaacgtga a
//
LOCUS       DAZULG010000331          909 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5204
            SAMEA115707565-rid22576633.guided.291, whole genome shotgun
            sequence.
ACCESSION   DAZULG010000331 DAZULG010000000
VERSION     DAZULG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707565
            Sequence Read Archive: ERR13197294
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288069.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288069.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:07:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,099
            CDSs (total)                      :: 3,037
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 48 of 87
            Pseudo Genes (internal stop)      :: 24 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707565-rid22576633.guided.291"
                     /isolate="VRE5204"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            296..844
                     /gene="aac(6')"
                     /locus_tag="ACG9CY_003099"
     CDS             296..844
                     /gene="aac(6')"
                     /locus_tag="ACG9CY_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4939502.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          328 a          129 c          187 g          265 t
ORIGIN      
        1 taaaaattaa gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt
       61 ttaaagcaaa agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg
      121 gctttcctga tctttcctat tttgaataaa gtgtttctgt ataatttttt attttataag
      181 taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa
      241 catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat
      301 aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag
      361 actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa
      421 tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc
      481 aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg
      541 acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag
      601 aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca
      661 aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca
      721 tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa
      781 tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca
      841 ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga
      901 aaattactt
//
